We're way into the holidays and the trendy stockholmers in their summergear leave every thought of time behind but wear their wristwatch as a stylish accessory. Something eye catching to jazz up your shorts and tunic! Swedish designstudio Form Us With Love bring their new watch for Tid straight into the contemporary by exploring a classic Scandinavian design value, eg transparency. And, as the trend goes, leaving leather behind. Their TID N0 3 is both a qualitative tool for urban life and a trend marker.
"Tid" is the Swedish word for "time". And a Swedish brand for watches. TID No 3 watch by Form Us With Love for Tid (2017) is made of light weight, durable plastic and comes in a range of colours. Photo copyright Tid.
TID No 3 watch by Form Us With Love for Tid (2017). Silicone wristband comes in colours black, grey, green, blur, pink and orange. One size: 38 mm. Photo copyright Tid.
TID Nylon Wristband by Form Us With Love for Tid (2017) comes in several colours and is available with black, gold or silver buckle. Photo copyright Tid.
Plank is a sound absorbing, fire retardant wood/wool tile by Form Us With Love for Baux (2016). During a visit to the Träullit factory where the tile is manufactured FUWL learned that the factory turns off their machines on weekends in order to be economical and environmental friendly. When production start again on Mondays, with cold machines, the first tiles comes out in a light colour. And then, for each day of the week, the tiles comes out slightly darker due to accumulated warmth in the machine. The subtle, natural colours actually depends on the temperature of the machines! From this unexpected aesthetical value Form Us With Love decided to work with the natural colours in different combinations, creating interesting surfaces. How handsome! And very contemporary!
Baux Plank Reception, acoustic tile by Form Us With Love for Baux (2016). Photo copyright Form Us With Love.
Baux Plank Stairs, acoustic tile by Form Us With Love for Baux (2016). Photo copyright Form Us With Love.
Baux Plank Study, acoustic tile by Form Us With Love for Baux (2016). Photo copyright Form Us With Love.
With less than a week to go before Stockholm Design Week kicks off, interesting previews of news are piling high. During the 2016 Stockholm Furniture & Light Fair I'll be especially curious of what the Scandi brands are up to!
The most hyped launch must be Pause for Harmony by Issey Miyake for Iittala. The master of pleating and plissé goes Finnish!
Another top notch collaboration is fab Ingegerd Råman for Ikea. Viktigt collection will be presented on February 11th. Same date as the release of "It's nothing but it's still something", a book about the designer and her work.
Swedese with news by Claesson, Koivisto, Rune, Broberg & Ridderstråle, Roger Persson and…Barber & Osgerby, the brilliant Brittish design duo who're "2016 Guests of Honour" at the Fair.
Claesson, Koivisto, Rune/ Smaller Objects and their everyday objects designed by a range of well known international designers.
Gärsnäs with news by David Ericsson (I'm still stunned by the beautiful Madonna Chair he created for Gärsnäs last year), and Färg & Blanche, to name a few.
Form Us With Us celebrates 10 years as a design studio with an exhibition at Konstakademien.
Fogia goes Seth Andersson; Carina Seth Andersson is a strong addition to the Fogia team, where we also find gifted designers like Andreas Engesvik (Tiki sofa, 2015). Furthermore, Fogia is launching a life style concept under the creative direction of Note Design Studio.
And Note Design Studio themselves exhibit at ArkDes, the Architechture and Design center at Skeppsholmen in Stockholm.
There'll be no lack of good, environmental friendly and visually sustainable design. As usual, Sanna and I will attend the Fair as well as events in town. We'll walk and talk and mingle…and report back to you!
The Forest Project – BAUX Acoustic Panels from BAUX on Vimeo.
Swedish design team Form Us With Love has an appealing easy-going approach to interiors and design; they stay ground with their credo on focusing on the actual person who shall use their design. FUWL don´t hesitate to use words like "practical","simplicity" or "everyday life" when describing their design. These new products for Danish brand Menu was launched at Maison et Objet in Paris. LOVEly!
The Cage Collection by Form Us With Love for Menu, coat painted steel and ash wood, first shown in september 2014.
The Form Us With Love team outside their studio/showroom in Stockholm.


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RIP Lil Bub: Mike Bridavsky bids adieu to 'purest, kindest and most magical living force'
The puss garnered over 3 million followers on Facebook and 2.3 million-strong fanbases in Instagram while around 3,40,000 subscribers on Youtube viewed updates about Lil Bub's life.
By Safvana Yasmine
December 3, 2019 11:25 +08
TOP 10 ALL TIME POP STARS
Lil Bub, the Bloomington celebrity cat who took over the internet in late 2011 and won a place in the hearts of zillion netizens, passed away at the tender age of 8 at her home in Indiana. According to the Instagram update by her owner Mike Bridawsky, the little feline sensation left this world on Sunday morning. "... we lost the purest, kindest and most magical living force on our planet," Bridawsky mourned in a post with a picture collage containing the first and last photo of Bub and him.
The internet-famous puss garnered over 3 million followers on Facebook and 2.3 million-strong fanbases in Instagram while around 3,40,000 subscribers on Youtube pinned on to view updates about Lil Bub's life.
Bridawsky mourns Lil Bub
The peewee cat, who died in her sleep peacefully, left a lasting impression in many of lives. Bridavsky, in his lengthy post-cum-eulogy, remembered the best of the memories the pet-owner duo shared. Even though Bub had been "battling a persistent and aggressive bone infection," her death came in as an uninvited guest, Bridavsky wrote.
In an attempt to come to terms with the loss, he added: "I truly believe that she willingly made the decision to leave her failing body so that our family would not have to make that difficult decision ourselves."
Bridavsky, who calls himself Bub's caretaker, thanked his little magic fur ball for her unconditional love and the happiness she brought into the lives of many across the globe. "I am forever honored and humbled that you chose me as your caretaker," he wrote in his parting words to his fondest pet.
"And even though my heart is absolutely crushed by her graceful departure from planet Earth, I know that her sprit, magic, and overwhelming energy are still with us, reminding us every day to be better," he added fondly.
Lil Bub for a cause, Lil Bub a hero
This year marked a few big events for Bub and the cause she represented. On April 7, she made a special appearance at the inaugural function of the Catsbury Park Cat Convention organized in Asbury Park of New Jersey. The most common face at Catcon in California, Bub, hosted her last fundraiser for Tails from the City in Cleveland earlier last month.
Bub and music were long-time pals. From releasing her own album Science & Magic: A Soundtrack To The Universe and performing alongside Andrew WK to hosting celebrities at her talk show, Bub left a large imprint beyond geographical lines.
Poster animal for PETA
Bub, who loves a hearty meal after a long day of meet-greet with fans, became the poster animal for PETA's spay and neuter initiative for pets. Various shelters across the U.S. received donations and a percentage of Bub's merchandise sales over a period of 8 years.
As per the post by Bridavsky, along with her personal challenges, she took up the cause of other animals in need and helped raise more than $700,000. Along with becoming an inspiration to a national fund meant for the special requirements of pets, Bub became the subject of a breakthrough genetic and biological research.
Apart from this, the Tree House Humane Society received a donation of $1,800 raised at the screening of Lil Bub & Friendz at Metro Chicago. On September 3, 2013, Books-A-Million published Lil Bub's Lil Book: The Extraordinary Life of the Most Amazing Cat on the Planet. Bub also has her own online merchandise store and a weather app!
Eight long years of Bub-story
Bridavsky found the little feral near his home in Bloomington in the year 2011. Born with various genetic mutations, Lil Bub was a "perma kitten" suffering from extreme feline dwarfism. Bub's tongue hung out her mouth due to toothlessness and recessive lower jaw. According to scientists, Lil Bub has a mutation in gene RANK/TNFRSF11A. Apart from these health issues, the feline midget was also diagnosed with osteopetrosis in late 2012. Bub was battling a "passive-aggressive bone infection" before she bid adieu to this world.
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This is my first photo with BUB next our last photo together. On the morning of Sunday, December 1st 2019 we lost the purest, kindest and most magical living force on our planet. BUB was cheerful and full of love laying in our bed with us Saturday night, but unexpectedly passed away peacefully in her sleep.. I have always been fully transparent about BUB's health, and it was no secret that she was battling a persistent and aggressive bone infection. Even knowing this, we weren't expecting her to pass so soon or so abruptly without warning. I truly believe that she willingly made the decision to leave her failing body so that our family would not have to make that difficult decision ourselves. It is impossible to put into words the profound effect that BUB has had on my life, on the lives of thousands of homeless pets, and on the lives of those of you that have cared for her as if she were your own family. She taught me everything that I know about unconditional love, she brought my wife Stacy and I together, she's the reason we have our beautiful children Rosco and Lula, and she has been a constant source of warmth and love in our lives for the past 8 years. To say that our family is devastated would be an understatement. But most importantly, BUB has made a huge difference in the world of animal welfare, and in the lives of millions of people worldwide. She has literally saved thousands of lives (both pets and humans), she started the first national fund for special needs pets, she was the subject of groundbreaking genetic and biological research, she's helped raise over $700,000 for animals in need, and has spread a message of determination, positivity, and perseverance to people all over the world. And even though my heart is absolutely crushed by her graceful departure from planet Earth, I know that her sprit, magic, and overwhelming energy are still with us, reminding us every day to be better. Dearest BUB, I will never forget your generosity, your limitless supply of love, or your uncanny ability to bring so much magic and joy to the world. I am forever honored and humbled that you chose me as your caretaker. Please visit all of us in our dreams o
A post shared by Lil BUB (@iamlilbub) on Dec 2, 2019 at 9:25am PST
Mike Bridavsky's post announcing Lil Bub's death on December 1, 2019.
Lil BUB & Friendz is an award-winning documentary about BUB's unexpected climb to fame, and the popularity of cats on the Internet. Filmed during the fall of 2012, it takes place when BUB was only 1 year old just as she was gaining popularity. It won an award at the 2013 Tribeca Film Festival, but was never officially released or screened. Now, 6+ years later, you can watch the whole thing on BUB's YouTube channel at the link in our bio. #goodjobbub #lilbub
A post shared by Lil BUB (@iamlilbub) on Sep 9, 2019 at 12:42pm PDT
As you may know, BUB has an astounding appetite. She weighs 3.5 pounds and eats 1.5 cans of Halo pet food a day (8 ounces). So that would be like me eating 70 cans, or 24 pounds of Halo pet food... A DAY. At least she's getting the good stuff. #wowbub #goodjobbub
A post shared by Lil BUB (@iamlilbub) on Jul 21, 2019 at 12:01pm PDT
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redpajama_set_name: RedPajamaCommonCrawl


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Gadgets | September 16, 2020 10:47 am
Deal: These Are Your Best Apple Watch and iPad Discounts
If you're fine with an older or refurbished model, there are several current sales
Previous versions of the Apple Watch and iPad Air are on sale
By Kirk Miller
Please note: If you buy through the links in this article, we may earn a small share of the profits.
Apple threw a big event yesterday, touting their new versions of the Apple Watch and iPad.
One thing we noticed: Some of the new variations are affordable! And some, not so much.
If you can handle a previous generation's version of either device, there are some great sales going on right now. To wit: The Apple Watch Series 3 is down to $169-$199 on Amazon (depending on whether you go for the 38 or 42mm). That's tied with the lowest price we've ever seen. And it's still a really good activity tracker — even for swimming — that'll pair nicely with your iPhone.
BUY IT HERE: $169
If you can handle refurbished, Woot is hosting a one-day blowout on the iPad Air 2, which'll run you about $220-$280 depending on your memory needs. It's still a recent enough machine to run the new iOS 14, and the Retina display is outstanding (hint: If you need more time to decide, you can find similar deals on a refurbished iPad Air 2 at Back Market.)
BUY IT HERE: $220-$280
Prepare for Spring With Patagonia's Very Rare Sale on Baggies
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Spotify and Hulu have teamed up to offer a new bundle deal that includes Spotify's premium ad-free tier, and Hulu's with limited commercials. The deal will be available for $4.99 per month to US undergraduate students age 18 or older at an accredited college or university. This is a $13 discount, and the two companies plan to split the subscription fee, according to Business Insider.
The new bundle deal may be indicating a future of subscription services partnering with each other to have a wider audience. In fact, there are already some partnerships preceding Spotify and Hulu: AT&T has DirecTV and HBO, Verizon has the NFL and Sprint is pushing Tidal. T-Mobile just recently announced a new offer for free Netflix. These competing partnerships may be the driving force behind Spotify and Hulu's joint deal.
Though this may be true, the teaming up of Spotify and Hulu can help the other stand out. The bundle better positions Spotify to retain its leadership among music streaming services, with over 60 million paid subscribers as of July 2017, while its closest competitor, Apple Music, had 27 million subscribers as of June 2017. Adding access to video content through Hulu could help Spotify stay in the lead.
Hulu, on the other hand, can shore up to competitors in the subscription video on demand realm. They need a larger amount of video subscribers to keep up with Netflix, and Spotify's music streaming catalog gives Hulu just that — consumers who purchase the bundle will have a music streaming option in addition to video programming. This is especially enticing to college students that are deciding between video streaming services. Nonetheless, Hulu still may have a way to go to inch up to the insanely popular Netflix, with over 100 million subscribers globally, while Hulu last reported having 12 million in May 2016.
Meanwhile, this partnership can also help advertisers reach younger demographics like college students. "In bringing Spotify and Hulu together, we're now able to offer students – both the millions already on Spotify Premium, and those who are new to Spotify – access to the world's best music, TV and movie content in the simplest possible way," said Alex Norstrom, Spotify's chief premium business officer in a statement.
Will this quickly become the default college streaming pair, or will Netflix continue to prevail over binge-watching students everywhere? Only time will tell. Go to https://www.spotify. com/us/student in order to sign up for the student-discounted Spotify and Hulu bundle.


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Govt invites suggestions for inclusion of hydrogen-CNG as automative fuel
The suggestions or comments in this respect can be sent to the Joint Secretary (MVL), Ministry of Road Transport and Highways, within 30 days from the date of notification i.e, July 22, it said
New Delhi: In another step towards promoting green fuel, the government on Thursday said it has invited suggestions from stakeholders for inclusion of hydrogen-enriched CNG as an automative fuel. The Ministry of Road Transport and Highways has issued a draft notification seeking comments and suggestions from the public and all stakeholders for amendment to the Central Motor Vehicles Rules 1979 for inclusion of hydrogen-enriched CNG as an automotive fuel.
"This is another step by the Ministry for promotion of Green fuels for automobiles in the country," a statement from the Ministry said.
The suggestions or comments in this respect can be sent to the Joint Secretary (MVL), Ministry of Road Transport and Highways, within 30 days from the date of notification i.e, July 22, it said.
Earlier this month, the Ministry issued a draft notification to include motor vehicles of Categories M and N, running on compressed gaseous hydrogen fuel cell, to be in accordance with AIS (automative industry standard) 157:2020.
Further, the hydrogen fuel specifications for fuel cell vehicles will be in accordance with ISO 14687 till the corresponding BIS specification is notified under the Bureau of Indian Standard Act, 2016, it had noted. NAM
Bureau of Indian Standards
the ministry of road transport
the bureau of indian standard act
International Organization for Standardization
gaseous hydrogen fuel cell


Why is everyone crowing about kale? Because it's really awesome! Check out the following health benefits of eating kale and some delicious kale recipes!
Kale is a sturdy, leafy green plant related to broccoli, Brussels sprouts, cauliflower, and cabbage. It's rich in vitamins and cancer-preventing phytochemicals called glucosinolates. And because kale is deep green in color, it also contains lutein and zeaxanthin, two more powerful antioxidants that are related to vitamin A.
We recommend that you eat kale two or three times per week, either raw (as a green in salads, for example) or cooked.
Kale is very rich in vitamin K and vitamin A (as the precursor of beta carotene, which is also an antioxidant). Vitamin K is important for strong bones and normal blood clotting, and vitamin A is necessary for vision, cell reproduction, and fighting infections. Kale is also a good source of potassium, folate, and magnesium, so kale is good for a healthy heart.
Managing your weight: Kale is nutrient dense, which means it has lots of nutritional value and is low in calories. Kale has more fiber than other greens, so it keeps you full longer and you're comfortable eating less food.
Preventing cancer: Cruciferous vegetables like kale contain the glucosinolates that help to prevent cancer. The International Journal of Cancer reported in 2007 that eating a diet rich in kaempferol (one of the gluconinolates found in kale) is associated with a decreased risk of ovarian cancer.
Building stronger bones: The vitamin K in kale helps to regulate osteocalcin, a hormone involved in bone formation. Kale also contains magnesium and calcium, although the absorption of the calcium is inhabited by the presence of oxalic acid.
Feeling more energetic: Kale is a good source of iron, which is needed for healthy blood cells that can carry plenty of oxygen and glucose (the fuel your body needs for energy) to all your cells.
Waging war against infections: Kale contains a full day's worth of vitamin C and a lot of beta carotene. These nutrients help your immune system function properly by regulating the white blood cells that fight infections.
Protecting your eyesight: Lutein and vitamin C were shown by the Age Related Eye Disease Study in 2001 to slow down the effects of macular degeneration, the leading cause of blindness in the elderly.
Try these delicious kale recipes!
In a small bowl, whisk together sugar, olive oil, balsamic vinegar, and Worcestershire sauce.
In a large bowl, combine the strawberries, spinach, almonds, and sesame seeds. Just before serving, add the dressing to the salad, and toss to combine.
Preheat oven to 375 degrees Fahrenheit. Rinse the kale under running water and shake to dry. Tear the leaves into smaller pieces, and remove and discard tough rib sections.
In a small bowl, whisk together the olive oil, garlic, salt and pepper.
In a large bowl, toss the kale leaves with the olive oil mixture. Spread leaves on a baking sheet. Bake for 15 to 20 minutes, turning the kale every 7 to 8 minutes. It's done when the leaves are crispy and bright green with just a little brown around the edges.
Tip: Roasted kale keeps well in an airtight container for two days.
Per serving: Calories 132 (From Fat 70); Fat 8g (Saturated 1g); Cholesterol 0mg; Sodium 346mg; Carbohydrate 15g (Dietary Fiber 5g); Protein 5g.


Aberrant DNA methylation of regulatory genes has frequently been found in human breast cancers and correlated to clinical outcome. In the present study we investigate stage specific changes in the DNA methylation patterns in order to identify valuable markers to understand how these changes affect breast cancer progression.
Quantitative DNA methylation analyses of 12 candidate genes ABCB1, BRCCA1, CDKN2A, ESR1, GSTP1, IGF2, MGMT, HMLH1, PPP2R2B, PTEN, RASSF1A and FOXC1 was performed by pyrosequencing a series of 238 breast cancer tissue samples from DCIS to invasive tumors stage I to IV.
Significant differences in methylation levels between the DCIS and invasive stage II tumors were observed for six genes RASSF1A, CDKN2A, MGMT, ABCB1, GSTP1 and FOXC1. RASSF1A, ABCB1 and GSTP1 showed significantly higher methylation levels in late stage compared to the early stage breast carcinoma. Z-score analysis revealed significantly lower methylation levels in DCIS and stage I tumors compared with stage II, III and IV tumors. Methylation levels of PTEN, PPP2R2B, FOXC1, ABCB1 and BRCA1 were lower in tumors harboring TP53 mutations then in tumors with wild type TP53. Z-score analysis showed that TP53 mutated tumors had significantly lower overall methylation levels compared to tumors with wild type TP53. Methylation levels of RASSF1A, PPP2R2B, GSTP1 and FOXC1 were higher in ER positive vs. ER negative tumors and methylation levels of PTEN and CDKN2A were higher in HER2 positive vs. HER2 negative tumors. Z-score analysis also showed that HER2 positive tumors had significantly higher z-scores of methylation compared to the HER2 negative tumors. Univariate survival analysis identifies methylation status of PPP2R2B as significant predictor of overall survival and breast cancer specific survival.
In the present study we report that the level of aberrant DNA methylation is higher in late stage compared with early stage of invasive breast cancers and DCIS for genes mentioned above.
Breast cancer is the most common form of malignant disease in women worldwide and also the principal cause of death from cancer among women globally. In Norwegian women breast cancer accounts for approximately 23% of all cancers and ~800 women die as a result of the disease every year. Breast cancer is a heterogeneous disease with distinct histopathological, genetic and epigenetic characteristics. Epigenetic regulation is critical for normal growth and development and provides a layer of transcriptional control. Epigenetic alterations which occur in transformed cells involve changes in DNA methylation including global hypomethylation and focal hypermethylation, histone modifications and nucleosomal remodeling . Epigenetic changes are considered to be an early event in tumor development and one of the hallmarks of cancer . The degree of DNA methylation in the promoter region of tumor suppressor genes, transcription factors and drug response genes may play a role in the initiation of cancer, tumor progression and response to treatment. Identification of early epigenetic changes in breast cancer might give valuable markers for early detection and contribute to the understanding of how these changes affect the progression of the disease and prognosis for the patient. We previously described treatment-specific DNA methylation patterns in 432 CpGs in the promoter regions of 14 genes in samples from 75 patients with locally advanced breast cancer treated with doxorubicin . Further, we identified four novel genes (ABCB1, FOXC1, PPP2R2B and PTEN) that were found to be already aberrantly methylated in DCIS (Ductal carcinoma in situ), a pre-invasive stage of breast cancer  and that were also found to be methylated in the locally advanced breast cancers . These findings raised the question if DNA methylation patterns evolve between the different stages of breast cancers and pre-neoplastic lesions or are similar and independent of tumor stage. In the present study, using the same candidate gene approach as previously described [3, 4], we performed a large-scale analysis of 12 candidate genes and determined quantitatively the DNA methylation patterns of the 12 genes in 238 breast cancer patients of all stages from early premalignant DCIS to advanced metastatic disease. In addition to the analysis of stage dependent DNA methylation patterns, associations between additional clinico-pathological factors of breast cancer such as grade and ER status and DNA methylation patterns of these 12 genes were assessed.
A total of 238 samples were included in present study: 75 patients with locally advanced breast cancer that were enrolled in a prospective study evaluating predictive factors for response to doxorubicin  and 35 patients treated with 5-FU and Mitomycin for locally advanced breast cancer , patients in both cohorts were admitted to the Haukeland University Hospital in Norway between 1991 and 2001; 57 samples from a series of 212 breast cancers samples collected from Ullevål University Hospital (Norway) between 1990 and 1994 ; 71 tumors from a population-based cohort including 854 women diagnosed between 1986 and 2004 in Uppsala, Sweden with either: a) pure ductal carcinoma in situ of the breast (DCIS), b) an invasive breast cancer, 15 mm or less, without an in situ component or c) a mixed lesion, i.e., a lesion with both an invasive- and an in situ component . Clinical and molecular characteristics of the tumors are given in Table 1. DNA from six normal breast tissues was included to identify the DNA methylation baseline in normal tissues. Normal breast tissue was obtained from women who underwent a biopsy of the mammary gland because of mammographic screening and for whom histology confirmed the presence of only normal tissue. All patients had given informed consent, and the project was approved by the local ethical committee.
Unknown data was excluded from total when the percentage was calculated.
DNA concentrations were determined using the Quant-iT™ dsDNA broad range assay kit (Invitrogen, Cergy Pontoise, France) and normalized to a concentration of 50 ng/μl. One μg of DNA was bisulphite converted using the MethylEasy™ HT Kit for Centrifuge (Human Genetic Signatures, North Ryde, Australia) according to the manufacturer's instructions. Quantitative DNA methylation analysis of the bisulphite treated DNA was performed by pyrosequencing or - in case of several sequencing primers - by serial pyrosequencing . Oligonucleotides for PCR amplification and pyrosequencing (Additional file 1) were synthesized by Biotez (Buch, Germany) . In the present study, same candidate gene approach was used as previously described [3, 4] with the difference in number of covered CpGs (205 in our case) because of absence of variability. These genes were initially selected on the following basis: previous reports of DNA methylation in breast tumors or at least breast cancer cell lines (ABCB1, BRCA1, CDKN2A, ESR1, GSTP1, IGF2, MGMT, MLH1, PPP2R2B, PTEN, RASSF1A) and genes displaying variation in breast cancer gene expression profiles (FOXC1).
The average value of methylation for all CpGs in a target region was calculated for each sample and each gene. Although there was some stochastic variation between different CpG positions, the overall methylation level was quite constant and CpG positions were highly correlated in the analyzed regions. A sample was considered 1) hypermethylated if the percentage of DNA methylation was higher than the sum of two times the standard deviation and mean of the normal samples, 2) normal-like methylation if the% DNA methylation was in range of two times the standard deviation +/− mean of the normal sample and 3) hypomethylated if% DNA methylation was lower than two times standard deviation – mean of normal sample. The aggregated quantitative DNA methylation data is presented in Table 2. Differences in the presence of methylation were determined by a two-sided Fisher test (for variables with two categories) and Chi- squared tests for variables with three or more categories. Odds ratio and 95% confidence intervals were calculated for two-categorical variables. Differences in the distribution of methylation were assessed by the non-parametric Mann–Whitney test (on parameters with 2 categories) or the Kruskal-Wallis test analysis on parameters with more than two categories. All obtained p-values were corrected with the Bonferroni correction method in which the p-values are multiplied by the number of comparisons. The methylation index of samples (Z-score) was calculated as: (methylation level of each sample – mean value of methylation levels)/SD of methylation levels. Then the sum for the 12 genes was calculated giving one single value (Z-score) for each sample. The false discovery rate was not considered in this study due to small number of genes which were tested. All calculations were performed using Statistical Package for Science version 18.
Univariate, Kaplan-Meier analyses and the log-rank test for each parameter for single gene was performed to investigate which genes and parameters affect survival. Further, multivariate, the Cox proportional hazard model was used to identify independent prognostic markers for all genes and from all clinical parameters: age, stage, tumor size and grade, lymph node status, TP53 mutation status, ER, PR status, T status. Methylation status of all genes was treated as continuous and categorical. We constructed possible model candidates using all combinations of given variables. To select the best-fitted model from all candidates, we evaluated the Akaike Information Criterion (AIC) . AIC gives an evaluation for model selection, which is modified by a penalty increasing with the number of variables of the model. Analyzing all combinations of given variables we selected the model that fitted best to the data indicated by a minimal value for the AIC.
A total of 48790 epigenotypes were generated through analyses of 205 CpGs in 12 genes (ABCB1 (20 CpGs), BRCA1 (19 CpGs), CDKN2A (28 CpGs), ESR1 (21 CpGs), FOXC1 (9 CpGs), GSTP1 (21 CpGs), IGF2 (17 CpGs), MGMT (9 CpGs), MLH1 (16 CpGs), PPP2R2B (14 CpGs), PTEN (19 CpGs) and RASSF1A (12 CpGs)). Six normal samples were used to estimate the normal-like methylation levels for all analyzed genes. Our analysis showed that five genes ABCB1, FOXC1, GSTP1, PPP2R2B and RASSF1A were the most frequently hypermethylated genes in all invasive samples as well as in the DCIS samples. PTEN was hypermethylated in invasive cancer of stage II, III and IV and MGMT was hypomethylated in invasive tumors of stage II, III and IV. CDKN2A had a normal-like methylation level in a high percentage of the DCIS samples and early stage tumors. In late stage tumors CDKN2A showed higher percentage of hypermethylated samples compared to the early stage tumors and the DCIS. The methylation levels of ESR1 and MLH1 were normal-like both in the DCIS and the invasive tumors and BRCA1 had a normal-like methylation level in almost all the DCIS and all invasive tumors (Table 3.).
Samples were considered as hypermethylated if the% DNA methylation was higher than the sum of two times the standard deviation and mean of the normal samples and hypomethylated if% DNA methylation was lower than two times the standard deviation - mean of the normal samples.
Significant differences in methylation levels between the DCIS and invasive stage II tumors were observed for six genes RASSF1A, CDKN2A, MGMT, ABCB1, GSTP1 and FOXC1 (p = 0.008, p = 0.005, p = 0.003, p = 0.006, p = 0.010, p = 0.010 respectively). RASSF1A, ABCB1 and GSTP1 showed significantly higher quantitative methylation levels in late stage compared to the early stage breast carcinoma. The most significant differences in methylation levels for these three genes were between stage I and III (p = 0.001, p = 0.022, p = 0.019 respectively) and between stage I and IV (p = 3.3e-6, p = 0.030 and p = 0.014 respectively). PTEN and CDKN2A methylation levels were low and increased in late stage III and IV (p = 0.003, p = 0.004 between stage I and III, p = 0.018 and p = 0.003 between stage I and IV), while MGMT methylation levels were low and appear to decrease with tumor stage (p = 4.5e-4 between stage I and IV) (Figure 1). After correction for multiple testing (Bonferroni correction), differences in methylation levels for RASSF1A between stage I and III and stage I and IV remained significant. Absolute differences in mean methylation levels for RASSF1A were higher than 10%. For PTEN, CDKN2A, MGMT methylation levels between stage I and III and stage I and IV remained significant after correction. Differences in methylation levels between the DCIS and invasive stage II tumors for MGMT also reached statistical significance after correction. Absolute differences in mean methylation levels between different stages for PTEN, CDKN2A, MGMT were less than 3% even though they remained significant after Bonferroni correction. We next combined all the methylation data into a single variable, the methylation index, to investigate further, how methylation levels change during progression of breast cancer. At the same time we wanted to investigate the presence of general pattern which might be more robust than single genes. As shown in Figure 2, we observed significantly lower methylation levels in DCIS and stage I samples compared to stage II, III and IV samples (p = 3e-7). Significant differences in methylation levels were observed between the normal breast tissue and stage II, III and IV tumors (p = 0.001, p = 0.006, p = 0.009 respectively). Normal breast tissue showed lower levels of methylation compared with tumors mentioned above. There was no significant difference observed between the normal samples and the DCIS and stage I tumors. Also grade 2 and 3 tumors had significantly higher Z-scores than grade 1 tumors (p = 0.022). Methylation index analysis showed that Luminal A and Luminal B tumors had significantly higher Z-scores than Basal-like tumors (p = 0.007).
Average percentage of DNA methylation levels for all CpGs between normal, DCIS and invasive tumor samples (stage I, II, III and IV).
Boxplots illustrating significant association between methylation status and tumor stage and grade. (a) Significantly lower methylation levels in DCIS and stage I samples compared to stage II, III and IV samples was observed. (b) Also grade 1 tumors had significantly lower methylation index compared with grade 2 and grade 3 tumors.
We compared the DNA methylation profiles with the TP53 mutations status and found that tumors with TP53 mutations had significantly lower DNA methylation levels then tumors with TP53 wild type in RASSF1A, PTEN, PPP2R2B, FOXC1, ABCB1 and BRCA1 (p = 0.028, p = 0.031, p = 0.002, p = 0.017, p = 0.010, p = 0.001 respectively). After Bonferroni correction DNA methylation levels in PPP2R2B and BRCA1 were still significantly lower in tumors with TP53 mutations. Significant associations with (ER) estrogen receptor status were observed for RASSF1A, PPP2R2B, GSTP1 and FOXC1 methylation levels (p = 0.004, p = 0.012, p = 0.012, p = 0.032, respectively). HER2 receptor status was associated with RASSF1A, PTEN, MGMT, CDKN2A and ESR1 (p = 0.023, p = 3.6e-7, p = 1.1e-8, p = 5.8e-9, p = 0.017) and after Bonferroni correction PTEN, MGMT and CDKN2A remained significant. No significant association with (PR) progesterone receptor status was observed (Table 4). Mann–Whitney test revealed that ER and HER2 negative tumors had lower methylation levels compared with ER and HER2 positive tumors for all studied genes. Interestingly HER2 negative tumors had higher methylation levels of ESR1 compared with HER2 positive tumors (p = 0.017). Z-score analysis showed that TP53 mutated tumors had significantly lower overall methylation levels compared to tumors with wild type TP53. Also HER2 positive tumors had significantly higher z-scores of methylation compared to the HER2 negative tumors (Figure 3). There was no significant association with PR and ER status.
Indicated are % methylation within the studied subgroups together with p-values for differences in DNA methylation levels between genes in connection with ER, HER2 and TP53 mutation status. Mann–Whitney test.
Boxplots illustrating significant association between methylation status and HER2 and TP53 status. (a) TP53 mutated tumors had significantly lower overall methylation levels compared to tumors with wild type TP53. (b) HER2 positive tumors had significantly higher methylation index compared to the HER2 negative tumors.
To investigate which parameters contribute to differences in survival we applied: 1) univariate analysis using Kaplan-Meier modeling and the log-rank test for each gene and each clinical parameter and 2) multivariate analysis using Cox hazard proportional model to all variables. To select the best-fitted model from all model candidates, we evaluated the Akaike Information Criterion .
Univariate survival analysis identified methylation status of PPP2R2B as significant predictor of overall survival. As expected, grade, estrogen receptor status, TP53 status and stage also appeared as significant predictors of survival. The Kaplan-Meier plot (Figure 4) showed a significant difference in survival between hypermethylated and normal-like samples for PPP2R2B (p = 0.012) indicating that patients with hypermethylated genes had better survival. Breast cancer specific survival was significantly improved in patients with hyper-methylated promoters for PPP2R2B (p = 0.012).
Kaplan-Meier plots of overall survival for patients with normal-like or hyper methylated PPP2R2B promoter. The p value was calculated using a long-rank test.
Further, multivariate survival analysis was performed in order to investigate if any of the methylation markers were independent prognostic markers, using both, categorical and continuous methylation data. We constructed possible model candidates using all relevant parameters and methylation data. Using calculated AIC, the best model that fits to the survival data was assessed. For categorical methylation data AIC identified a model explaining survival, which included the methylation status of IGF2, GSTP1, estrogen receptor status, TP53, N status and stage. The estimated coefficients, the hazard ratio, the p-values, and the 95% confidence intervals of the hazard ratio were summarized in Table 5. For continuous methylation data the best model explaining survival included TP53, T status, N status, estrogen receptor status and methylation status of IGF2 and GSTP1. The estimated coefficients, the hazard ratio, the p-values, and the 95% confidence intervals of the hazard ratio were summarized in Table 6. For both, categorical and continuous methylation data IGF2 and GSTP1 (p = 0.009 and p = 0.014) were significant together with ER status (p = 0.008), TP53 status (p = 6.6e-5) and N status (p = 0.047). For these models, we also described statistical significance for the likelihood ratio, Wald and log-rank tests in the Tables 5 and 6.
The variables that the minimum AIC selected. ( p-values: 3.4e-7 for likelihood ratio test, 3.4e-4 for Wald test, 3.2e-6 for log-rank test).
Positive hazard ratios indicate an increased risk of dying from breast cancer and are calculated for the different covariates.
The variables that the minimum AIC selected. ( p-values: 1.2e-7 for likelihood ratio test, 5.8e-4 for Wald test, 6.7e-6 for log-rank test).
The aim of this study was to quantitatively determine the methylation levels in the promoter region of 12 genes in breast cancer patients of all stages and to investigate stage specific changes in tumors. In addition, we wanted to evaluate the association between clinico-pathological factors (ER, HER2 status, grade) including survival and methylation levels of these genes.
In the present study, five genes, ABCB1, FOXC1, GSTP1, PPP2R2B and RASSF1A were hypermethylated already in early stage breast cancer (stage I and II). For all five genes hypermethylation was also detected in DCIS suggesting that inactivation of these genes is a frequent event in the process of mammary tumorigenesis. We found that RASSF1A was hypermethylated in approximately 85% of all invasive tumors and DCIS, and our results are in agreement with such a high incidence of RASSF1A methylation [18–20]. RASSF1A is a putative tumor-suppressor gene. It belongs to an increasing list of tumor suppressor genes that are frequently inactivated by promoter methylation rather than by somatic mutations . Since we detected a constant hypermethylation of RASSF1A in all of the different stages of the breast carcinomas we can suggest that hypermethylation of RASSF1A is an early event during breast cancer pathogenesis and also the main mechanism of inactivation. In our study, GSTP1 was found to be hypermethylated in different stages of breast carcinomas, for early stages (I and II) our results are in agreement with previous reports [22, 23]. The frequency of GSTP1 promoter hypermethylation in stage III and IV (around 70%) was found to be higher than reported previously [23, 24]. It is known that GSTP1 plays a role in detoxification of potential carcinogens and that loss of the expression of GSTP1 will lead to DNA damage of breast cells and they will be more easily exposed to carcinogens . Loss of GSTP1 expression and its potential role in breast carcinogenesis was observed in high proportion of breast tumors . It appears that promoter hypermethylation is associated with loss of GSTP1 expression . Important question is when the promoter hypermethylation of the GSTP1 gene starts to play a role in tumor progression? We found that already in DCIS there is a high proportion of hypermethylated GSTP1 (58%), which indicates that GSTP1 promoter hypermethylation is an early event in breast carcinogenesis. From our analysis we observed that hypermethylation of ABCB1, PPP2R2B and FOXC1 is also an early event in breast carcinogenesis since our results indicate high level of hypermethylation of these genes already in DCIS which was reported before . We found frequencies of methylation for CDKN2A MGMT and MLH1 similar to previously published reports [27, 28]. According to our results, we could suggest that hypermethylation of CDKN2A is possible event leading to its inactivation in late stage breast cancers. PTEN is a tumor suppressor gene. Its product PTEN protein works as a negative regulator of the Akt pathway, leading to suppression of apoptosis and increasing cell survival . We suggest here that epigenetic silencing of PTEN might be an early event in initiation of cancer and also the mechanism of its inactivation. Next, combining all the methylation data into a single variable, the methylation index, we investigate how methylation levels change during progression of breast cancer. Our analysis showed that the methylation pattern of all genes included in this study is changing during breast cancer progression. DCIS and stage I tumors had similar methylation levels with no significant difference. Stage II tumors showed the most significant difference in methylation levels when compared with DCIS and stage I and then in stage III and IV methylation levels were lower but still significantly higher than in DCIS and stage I. It remains unclear how this process of dramatic change in stage II is achieved and driven further during breast cancer progression. To our knowledge only one group investigate stage dependent DNA methylation in breast cancer using DCIS and all four stages of invasive tumors . They identified 33 cancer specific genes that were either highly methylated in early stage breast cancer or showed stage dependent methylation pattern, lower methylation frequency in early stage breast cancers and a higher methylation frequency in late stage breast cancers. None of our selected genes were methylated in this study. Additional studies of the relationship between DNA methylation in tumors and tumor stages are necessary.
In the present study we showed the associations between DNA methylation levels of candidate genes and the TP53 mutation status, estrogen receptor status, and HER2 status. The TP53 tumor suppressor gene has a central role in cell cycle regulation, DNA repair and apoptosis, and a large number of reports have discussed the important role of TP53 alterations in breast cancer. Also, a number of studies have shown that breast tumors with TP53 mutations are strongly associated with poor prognosis and lacking methylation in a number of regulatory genes [31, 32]. Additionally, studies on different expression subtypes in breast cancer showed that different subtypes have a different underlying biology reflected in methylation and is strongly influenced by TP53 mutation status. It was shown that basal-like tumors are TP53 mutated and unmethylated [6, 33]. In present study we have identified 26,6% of breast tumors with TP53 mutations and significantly lower levels of DNA methylation in RASSF1A, PTEN, PPP2R2B, FOXC1, ABCB1 and BRCA1 compared to tumors with wild type TP53. Since in our study we had low number of basal-like tumors (37 out of 238) we cannot confirm association between TP53 mutated and unmethylated tumors and basal-like but most of the basal-like samples were TP53 mutated and had lowest methylation levels compared to other subtypes (data not shown). The status of ER and HER2 have been recognized as important prognostic factors in patients with breast cancer, in addition to a predictive marker for the response to treatment with endocrine and trastuzumab therapy. Identification of genes with subtype-specific methylation revealed that RASSF1A and GSTP1 were highly methylated in Lum B tumors . These two genes were reported previously to be significantly more methylated in ER- positive than ER-negative tumors . In the present study we showed the same trend in ER-positive tumors compared to ER-negative for four genes and RASSF1A and GSTP1 were among them. The same group reported that HER2 positive tumors had higher methylation level for these two genes compared to HER2-negative tumors, which is again in accordance with our study. Furthermore, in a study on methylation in breast cancer and breast cancer molecular subtypes it was shown that RASSF1A is hypermethylated in HER2 positive breast tumors (ERBB2 and luminal B) . In our study RASSF1A was hypermethylated in ERBB2 and luminal B tumors (data not shown). Taken all together, these results, suggest that methylation plays a significant role in the different breast tumor phenotypes.
We report here for the first time the PPP2R2B methylation status as significant predictor for breast cancer survival as well as for overall survival. PPP2R2B is a candidate tumor suppressor gene and it was shown that changes in DNA methylation of this gene contribute to its expression . Further, multivariate analysis showed that IGF2 and GSTP1 were independent prognostic markers. Recently, it has been shown that the absence of GSTP1 protein expression correlate with promoter hypermethylation and with improved survival in invasive breast cancer samples . Hypermethylation of GSTP1 is a well established biomarker for hormone dependent cancers. Our previous analyses suggest that methylation of GSTP1 in locally advanced breast cancer patients treated with doxorubicin was associated to survival . However, GSTP1 had no effect on treatment response.
Here we report aberrant methylation levels of ABCB1, FOXC1, GSTP1, PPP2R2B and RASSF1A in DCIS and stage I-IV providing evidence that suggests that changes in methylation level is an early event and may also be important in progression to later stages of breast cancer. We also report that methylation levels of important breast cancer genes are associated to hormone receptor status and TP53 mutation status suggesting mechanisms of deactivation of tumor suppressor genes in breast cancer. Further studies are necessary to identify which methylation gene profiles are of predictive and which of prognostic value.
The authors gratefully acknowledge the women participating in this study for their contribution. This work was supported by grant from The Norwegian Cancer Society (VNK), the Norwegian Research Council (VNK) and the French-Norwegian cooperation program Aurora grant (JT,VNK). JK is a fellow of the Akershus University hospital, Division of Medicine, University of Oslo. TF is a fellow of the Faculty Division the Norwegian Radium Hospital, University of Oslo. HE is a postdoctoral fellow of the Norwegian Cancer Society.
JK performed data analyses and wrote the manuscript. ED perfomed laboratory experiments.TF, HE and HS were involved in the statistical analyses. PEL and FW were responsible for the patient cohorts and IB for the control samples. ALBD, JT and VNK initiated and designed the study and participated in writing the manuscript. All authors have read and approved the final manuscript.


Today we visited Gaderummet (The Street Room) a place for homeless people. It's a unique place that is the only place like that in Denmark. We got to talk to some of the homeless people today and it was very interesting. They talked about were they lived before they got that house, they told us they lived in a small room in a house upstairs, there were many of them staying there and there was no room for them to sleep it was a very hard struggle. It took them 10 years to get a proper home so they could sleep and eat properly. The new house they are staying in now is bigger and more open with many rooms so there is a lot of space to use.
The door is always open for people who need to sleep and get something to eat. There are a few rules in the house. There are no hard drugs, no steeling and no violence.They are allowed to smoke hash and do bongs to relax. But no hard drugs. There has been a conflict with the authorities that Gaderummet allows people to smoke hash because it is illegal in Denmark. This makes them very unique because for people living in other institutions no drugs are allowed.
In Gaderummet they are very open about hash and because of that they can smoke it everywhere in the house. They don't have to hide it and because it is allowed they don't do hard drugs. They accept the 3 rules of the house and they take care of each other when it is needed and they also take care of the place and they take turns of cleaning up.
Everybody from other countries are welcome to stay there and it wouldn't be a problem. They fell very safe there and it's a anti-racial place. There are a few conflicts in the house but nothing serious. The only big conflict there is the ones between the two male dogs in the house who are fighting over a female dog. All animals are allowed.
Every Sunday there is a meeting around the table and if there are any arguments they will sort them out. Conflicts are very easily solved here. When there is a staff meeting they are allowed to join in and have their own opinion and there is a book in the main room that they write everything into it so there is no secrets and everybody can read it and know what is going on.
There is prejudice of fear from people outside. Some of the people in the house have been throw out of shops because the look poor and they think they are thieves because there looking at the food.
If they want to help themselves getting out of homelesness it's not an easy job. If you were to get an apartment it is 3500 kr. and they only get 6000 kr. a month so its hard to live in the month because you have to buy food and other stuff.
There are 25 people living in the house at the moment but other people come in once and a while to get some sleep and food. 5 of the people go to school and they have there own room so they can get some sleep at night because they have to get up early in the morning and 7 have a part-time job.
We were talking to one of the boys there, he is 20 and has been coming there for half a year. He comes and goes when he wants. He likes it there and gets on with most of the people there. He has been homeless a few years and has been in jail for 4 years for violence and other stuff. He has family but his mother doesn't talk to him. His father is the only person that talks to him.
Another guy was thrown out by his girlfriend and therefore he had no place to stay. At first he stayed in trains, laundries and under stairs. He heard about Gaderummet from a friend from Christiania who told him that, that was a place where everybody was welcome.
The workers that work there are trusted by the people because they don't trust the authority. The age group is 18-30. There are no under 18 people there because its not allowed. The volunteers that work there have stayed there before themselves and now that there on their own 2 feet they come back to help the others.
In the house there are 4 showers and 4 toilets so it's not a crush to get showered. Most of them share room there is about 5 or 6 to a room it's very busy.
In our opinion its very sad to see people like this because we have it much better than them. It really makes you think about how good we really have it. And we shouldn't take things for granted.


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The New Retail and Service Exemption: DOL Revokes Outdated Lists
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Retail, Wage and Hour
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Lisa Stephanian Burton Boston Author
Charles E. McDonald, III Greenville Author
Effective with the May 19, 2020, publication in the Federal Register, the U.S. Department of Labor's (DOL) Wage and Hour Division revokes the arbitrary lists it created in 1961 identifying industries that may, or would not, qualify as retail or service in nature "for purposes of an exemption from overtime pay applicable to commission-based employees." The final rule removed the lists contained at 29 CFR 779.317 and 779.320.
Agreeing with courts interpreting the guidance over the years that the lists are "incomplete, arbitrary, and essentially [a] mindless catalog," as noted in Alvarado v. Corporate Cleaning Services, Inc., 782 F.3d 365, 371 (7th Cir. 2015), the retail and service establishments lists now are revoked allowing all industries to review the exemptions criteria and potential application based on how they operate today and in the future.
The withdrawal of the two provisions from the WHD's regulations matters because the exemption in Section 7(i) of the Fair Labor Standards Act (FLSA) provides a mechanism for retail or service establishments to exempt certain employees from overtime requirements if three conditions are met. That requirement obliges employers to pay employees one-and-one-half of their regular rate of pay for all hours worked over 40 in a given week. According to the WHD's Fact Sheet #20, for an employer to qualify for the exemption "three conditions must be me:
the employee must be employed by a retail or service establishment, and
the employee's regular rate of pay must exceed one and one-half times the applicable minimum wage for every hour worked in a workweek in which overtime hours are worked, and
more than half the employee's total earnings in a representative period must consist of commissions."
While the new final rule certainly seems helpful to employers as it streamlines the definition of retail under the FLSA and removes the prior industry lists, employers still need to make sure they meet the definition of a retail or service establishment if they want to take advantage of the Section 7(i) overtime exemption. Employers that have historically not been viewed as being a retail establishment may want to use caution if they would like to avail themselves of the 7(i) exemption.
While the removal of the list of industries lacking a retail concept contained previously in 29 CFR 779.317 may provide some employers the opportunity to take advantage of the exemption, employers could still be subject to litigation over the retail nature of their businesses. Employers have the burden of establishing all three factors of the Section 7(i) exemption—the first being that the employee is employed by a retail or service establishment. There is no guarantee that this change in the regulations has really expanded the number of retail establishments, as the retail definition has not changed. A retail establishment is an establishment 75 percent of whose annual dollar volume of sales is not for resale and is recognized as retail in the particular industry. The WHD applies the analysis in 29 CFR Part 779.24 to all establishments when determining whether an establishment qualifies as a retail establishment. And, as always, employers are also subject to the state laws in which they operate.
Seventh Circuit Preaches on the Ministerial Exception
For the first time since a 2012 decision by the Supreme Court of the United States, the Seventh Circuit Court of Appeals elaborated on and applied the Supreme Court's four-factor analysis to determine whether a position is properly considered a ministerial roles. In its recent decision in Grussgott v. Milwaukee Jewish Day School, Inc., No. 17-2332 (February 13, 2018), the Seventh Circuit broke a 2-to-2 tie among the four factors and held that the circumstances in the case of a teacher at a Jewish school supported the conclusion that the teacher's position was subject to the ministerial exception.
Healthcare Workforce Resilience Act: Is Relief On the Way for Physicians and Nurses?
On May 5, 2020, a bipartisan group of senators, including Senator David Perdue (R-GA), Todd Young (R-IN), John Cornyn (R-TX), Dick Durbin (D-IL), Chris Coons (D-DE), and Patrick Leahy (D-VT), introduced the Healthcare Workforce Resilience Act (Senate Bill 3599). The goal of the proposed legislation is to temporarily address the country's shortage of doctors and nurses, strengthen the healthcare workforce, and improve healthcare access during the COVID-19 crisis.
Does Your Health Plan Violate the New Mental Health Parity Rules?
New regulations are giving employers their first glimpse of the mental health and substance abuse benefit changes that may be needed by 2011 to ensure that their health plans do not violate the Paul Wellstone and Pete Domenici Mental Health Parity and Addiction Equity Act of 2008 ("New Mental Health Parity Act"). Published in the


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[Màiri Rodgers – Preseantair] Tha dragh air nochdadh am measg coimhearsnachd Bharraigh gun toir na gearraidhean air a bheil a' Chomhairle a' beachdachadh barrachd buaidh an sin na bheireadh e mun cuairt Steòrnabhaigh. Chaidh coinneamh phoblach mu na sàbhalaidhean a chumail air an eilean a-raoir, far an do thog daoine draghan mu na seirbheisean saor-thoileach agus an t-seirbheis-adhair chun nan eileanan mu thuath. Tha an aithris seo aig Doneil MacLeòid.
[Doneil MacLeòid – Neach-aithris] Nochd còrr air trì fichead duine a chuir am beachdan mu choinneamh na comhairle air na molaidhean a chuir iadsan air adhart airson sàbhalaidhean buidseit. Bha cuid anns an sgìre ag ràdh gum biodh barrachd buaidh ann am Barraigh na dheigheadh fhaireachdainn nas fhaide gu tuath.
[Betty Nic an t-Saoir – Fuireach san sgìre] Tha e a' cur barrachd uallaich oirnn, saoilidh mi, airson ar guth a thogail agus airson leigeil leis a' chomhairle fios gu bheil e draghail dhuinn. Am beagan seirbheis a tha sinn a' faighinn, gum feumar a ghleidheadh cho math 's as urrainnear agus gu bheil na buill-comhairle, gu bheil iadsan cuideachd mothachail air na sgìrean beaga iomallach air am buail na gearraidhean fada nas cruaidhe na bhuaileas iad air àiteachan meadhanach mar a tha baile Steòrnabhaigh.
[Doneil MacLeòid – Neach-aithris] Tha a' chomhairle ag ràdh nach eil càil air a sgrìobhadh ann an clach fhathast agus gun gabhadh iad sùim ri na bha daoine ag ràdh.
[An Comh. Tormod Dòmhnallach - Comhairle nan Eilean Siar] Tha mi smaoineachadh gum feum sinne na gearraidhean a tha seo a dhèanamh tarsainn nan seirbheisean againn air fad agus bidh sinn gu h-àraidh a' coimhead ri far a bheil seirbheisean, far a bheil buaidh mhòr gu bhith aca ann an coimhearsnachd shònraichte, mas e 's gun tig an gearradh, agus bhithinn an dòchas gun deigheadh sin a sheachnadh nan gabhadh e dèanamh. Chan eil teagamh ann, bha cuspairean ag èirigh leithid còmhdhail anns a' choimhearsnachd, tha sin air a bhith air a thogail aig feadhainn de na coinneamhan eile a th' air a bhith againn, agus 's e sin rud a thèid sinn air ais thuige agus chì sinn dè a' bhuaidh dha-rìribh a bhios aig an sin ann an coimhearsnachdan fa leth a rèir 's mar a tha an t-seirbheis air a lìbhrigeadh an-dràsta.
[Doneil MacLeòid – Neach-aithris] Ach bha e soilleir bho cuid gun robh dragh ann mun dòigh a bha na gearraidhean gu bhith bualadh air seirbheisean ann an diofar dhòighean ann an diofar sgìrean.
[Dòmhnall Manford - Comhairliche ionadail] 'S e seo an rud a bha mise ag ràdh o thùs, an dòigh a tha a' chomhairle a' dol mu thimcheall air an seo. Chan eil e dòigheil idir agus thàinig sin gu math follaiseach a-nochd agus tha mi uabhasach toilichte gun tàinig daoine a-mach a dh'innse dhan fheadhainn a thàinig a-nuas cà' bheil iad a' dol ceàrr.
[Doneil MacLeòid – Neach-aithris] Bidh coinneamh phoblach eile a' gabhail àite ann an Dalabrog ann an Uibhist a Deas a-nochd mun bhuaidh a ghabhas na gearraidhean an sin. Doneil MacLeòid, BBC An Là, Barraigh.
[Mairi Rodgers – Presenter] Concerns have been raised by the Barra community that the council's proposed cuts will affect them more than the communities surrounding Stornoway. A public meeting about the proposed savings was held on the island last night, where people raised concerns regarding voluntary services and the air-service to the more northerly isles. Doneil MacLeod reports.
[Doneil MacLeod – Reporter] More than sixty people came to express their views on the council's budget saving proposals. A few locals feel that the Barra community will we more badly affected than those further north.
[Betty MacIntyre – Local resident] It puts more pressure on us, I think, to make our feelings known and to let the council know that we are concerned. We need to protect the few services we have as best we can and make sure that council members are mindful of the smaller, more remote communities that will be affected by the cuts, more so than larger town such as Stornoway.
[Doneil MacLeod – Reporter] The council says that nothing is yet written in stone and that they would take heed of what people are saying.
[Cllr Norman MacDonald - Comhairle nan Eilean Siar] I think these cuts need to be made across our full range of services and we will be looking in particular at where services are, where they will affect communities the most - if cuts are in fact required - and I would hope that these would be avoided if at all possible. There's no doubt, matters, such as community transport, have been raised at other meetings we have held, and that is a matter that we will be looking at again at and we can ascertain what effect it will have in individual communities and how these services are currently being delivered.
[Doneil MacLeod – Reporter] But it was clear that some people are concerned about how the cuts could affect services in different ways in different communities.
[Donald Manford - Local Councillor] This is what I have been saying from the beginning, the way the council is dealing with this. It's far from ideal and that was quite obvious this evening and I am very pleased that people came out to let those who came down know where they are going wrong.
[Doneil MacLeod – Reporter] Another public meeting will take place in Daliburgh in South Uist this evening to discuss the impact these cuts will have there. Doneil MacLeod, BBC An Là, Barra.


text:
Home Scores Bracket Rankings Standings Stats Video Final Four History NIT
Andy Katz | NCAA.com Correspondent | February 19, 2018
Resurgent Wichita St., Baylor's Maston lead weekly honors
Terry Maston | Player of the Week
If it's Monday, then it's time for the Weekly Honors. Here's the best of the past week in the world of college basketball:
Wichita State is right back in the American race after an intense win over Cincinnati in its adopted home at Northern Kentucky University. Wichita State played the physical style that has been its trademark. The Shockers didn't back down. And they finished off a week where they also took out Temple at home, avenging a road loss to the Owls in Philadelphia.
Going into Cincinnati, No. 19 @GoShockersMBB tightened the race for the #AmericanHoops regular-season crown, knocking off No. 5 Cincinnati, 76-72, snapping the Bearcats' 39-game home court winning streak in the process.
Highlights from BB&T Arena: pic.twitter.com/XxZaociTEj
— American MBB (@American_MBB) February 19, 2018
Wichita State is tied with Houston, a game back of Cincinnati in the loss column. The Shockers get Cincinnati at home to end the regular season after playing Tulane and then at SMU and at UCF. The American wanted/needed Cincinnati and Wichita State to become a rivalry in year one. Mission accomplished.
Terry Maston, 6-8, Sr., F, Baylor: Maston scored 24 in the two-point win over Texas Tech and 26 in the double-overtime win at Texas earlier in the week. He was a combined 22-for-36 in the two games. Baylor has won five in a row, including a win over Kansas at home.
MARCH MADNESS SHOP
Maston has seen his numbers jump up from his junior to his senior year. He is yet another example of a Baylor player who developed into an effective offensive talent later in his college career. Baylor seems to always find the long, athletic wings who can be a tough matchup. Maston is helping the Bears make a late push toward an NCAA tournament berth.
The Backup Five
Fletcher McGee, 6-4, Jr., G, Wofford: McGee scored 45 in a road win at Chattanooga and 21 in a road win at Western Carolina. He made 11 threes in the win over Chattanooga and five in the win over Western Carolina. Wofford, which beat North Carolina in Chapel Hill, still needs to win the Southern Conference tournament to get into the field. But if the Terriers do, they've got a scorer who could cause problems in the first round.
MORE: How Fletcher Magee became one of the best 3-point shooters in NCAA history
Dylan Windler, 6-7, Jr., F, Belmont: Windler put up huge numbers in the win over Morehead State. He scored 36 points and grabbed 20 boards, making 14 of 17 shots, including 6 of 9 from deep. Belmont has won three in a row and is 22-7 overall. If Belmont can get in the field, Windler is another one of those players who could make himself a name in the NCAA tournament.
Matt Mobley, 6-3, Sr., G, St. Bonaventure: Jaylen Adams might end up being the A-10 player of the year, but Mobley was something special in the most important game in Olean in years. Mobley scored 26 and made five threes in the win over the previously streaking Rams. He also lit up La Salle on the road for 30 points earlier in the week. Mobley has played all 40 minutes in three straight games and four of the last five.
Watch. Rewind. Repeat. #Bonnies || #Unfurl pic.twitter.com/h2CJLXu9fk
— Bonnies MBB (@BonniesMBB) February 17, 2018
Yante Maten, 6-8, Sr., F, Georgia: Maten has given the Bulldogs a pulse in their quest to still make the NCAA tournament. He was instrumental in the two wins last week over Florida in overtime in Gainesville and against Tennessee. He scored 23 and grabbed 10 boards in the win over the Gators and had 19 and five in the win over the Vols. He has to be an anchor in the post for the Bulldogs if they're going to have a chance down the stretch.
Joel Berry II, 6-0, Sr., G North Carolina: Berry kept the Tar Heels going strong with 23 points, eight board and five assists in the road win at Louisville, and contributed 23 points, three boards and one assist in the convincing win over Notre Dame. The Tar Heels have won five in a row, including beating Duke at home and knocking off Louisville and NC State on the road.
Most impressive win
Villanova 95, Xavier 79: The Musketeers were jacked for this one. This was their chance to knock off Villanova and claim the top spot in the Big East. Not going to happen. Villanova got the sweep of Xavier and is on its way to win its fifth straight Big East title.
MORE: Predicting who's in and out of the NCAA tournament (Feb. 19)
Best win in a road environment
Arizona 77, Arizona State 70: The Wildcats' win in Tempe was extremely intense. Arizona State was desperate to beat its rivals to the south and re-assert itself as a possible Pac-12 tournament title contender. Arizona is going to win the Pac-12, but this was going to be the Sun Devils chance to prove themselves. But Arizona handled every run and got out of a hostile situation with a win and a sweep.
Most-needed road win
Syracuse over Miami: The Orange had won on the road, knocking off Georgetown, beating UConn at Madison Square Garden and taking down Pitt. But none of those teams are going to the tournament.
"Onions!" pic.twitter.com/wcNOVfBijZ
— Syracuse Basketball (@Cuse_MBB) February 18, 2018
The win at Louisville was a season-changer earlier this month, but Syracuse needed at least one more, especially with the upcoming games against North Carolina and at Duke this week. To get the win over Miami on the road and to do it by holding the Hurricanes to 55 points was a strong signal that this team can earn a spot.
Most surprising road win
Marquette 90, Creighton 86: The Golden Eagles had won at Providence. So, it's not like Marquette couldn't win on the road. But to do it at Creighton with Markus Howard (21.3 ppg) only playing 13 minutes due to an injury was surprising. Marquette made 11 3s and Sacar Anim had himself a game, making 11 of 16 shots for 26 points.
Bid-earning week
Houston beat Cincinnati at home. That was a must for the Cougars to show they belonged, getting the split of the season series. But that wasn't enough. Houston had to finish the week by winning on the road. And the Cougars did, destroying Temple, 80-59. Houston has won five in a row and seven of eight.
Most impressive road sweep
Nevada winning at Boise State and Utah State: The Wolf Pack went in front of a Taco Bell Arena crowd that was desperate to see the Broncos take down the Mountain West favorites.. But Nevada won by five and then followed that up by beating Utah State by six in Logan, the same spot where Boise State lost the previous week. Nevada is going to win the Mountain West and earn the top seed in the conference tournament. Oh, and this team should be in the NCAAs, even if it loses in Las Vegas.
Most stunning home win
What a night. #OnWisconsin pic.twitter.com/WR4zaGX13M
— Wisconsin Basketball (@BadgerMBB) February 16, 2018
Wisconsin 57, Purdue 53: The Boilermakers had lost two in a row coming into this game — in the final possession to Ohio State at home and at Michigan State. But Wisconsin had shown no sign it was capable of taking down the Boilermakers, getting blasted for a large portion of the home game against Michigan the previous Sunday before a late push to cut the final deficit to 11. Still, Wisconsin, suffering through its worst season in two decades due in large part to injuries to its two best guards, showed no quit. This team continues to find a way to fight for Greg Gard and each other.
Andy Katz is an NCAA.com correspondent. Katz worked at ESPN for 18 years as a college basketball reporter, host and anchor. He's covered every Final Four since 1992, and the sport since 1986 as a freshman at Wisconsin. He is a former president of the United States Basketball Writers Association. Katz can also be seen covering college basketball on Big Ten Network, Fox and FS1. Follow him on Twitter at @theandykatz. Follow his March Madness 365 weekly podcast here.
The views on this page do not necessarily reflect the views of the NCAA or its member institutions.
Here's how men's basketball teams with first-year head coaches are performing
Through the first three weeks of the season, teams with first-year coaches have improved defensively compared to last season.
Maryland's Mark Turgeon builds his ultimate dream player
Maryland head coach Mark Turgeon joins Andy Katz on the August 4 episode of March Madness 365. The two talk about racial injustice, the upcoming college basketball season and Turgeon's ultimate dream player.
The last undefeated men's basketball team from every season this century, ranked
Here are the final undefeated DI men's college basketball teams from every season since 2000, and a ranking of which of those 21 teams is the best.


Dantdsans21 @Dantdsans posted on @Dantdsans's wall.
Dantdsans21 @Dantdsans commented on this photo.
Dantdsans21 @Dantdsans replied to this post.
Dantdsans21 commented on this photo.
"the weird part is she touched herself with me pen who the f does that XD"
"NO YURI IS BEST BEST GIRL"
"when your crush admits they love you Yuri: Guess ill die"
Dantdsans21 commented on this post.
"sayori is a character from Doki Doki Literature Club a kind of horror game for more info type in DDLC or Sayori"
Dantdsans21 commented on this drawing.
"he was going to draw mario"


California Business and Professions Code Section 6061.7(c) requires that a law school shall publicly disclose on its Internet Web site, in a readable and comprehensive manner, all of the following information on a current basis.
In compliance with California Business and Professions Code Section 6061.7, the following information about Trinity Law School's JD program is provided. The Information Report for Reporting Year 2019, in accordance with Section 6061.7(a), is compiled from the latest available information.
©2019 Trinity Law School. All rights reserved.


(#92) The Proverbs of Solomon 15:4-You Listen to–What???
Since God Created humans, only God can provide specific understanding of human behavior. God gave Solomon Divine Wisdom (1 Kings Chapters 3 and 10) to explain what and why behavior is as it is, and Proverbs 10:1-24:34 are randomly written, as if they were Solomon's judgments about individual cases brought to him, or simply God-given explanations about life. New Testament passages may help see the continuation of Wisdom offered through Jesus Christ.
Words are vehicles for ideas, and they reveal the inner heart. Words cannot conceal the real person. "If anyone among you thinks he is religious, and does not bridle his tongue but deceives his own heart, this one's religion is useless" (James 1:26). Just as the prophet Elisha used some salt to purify the water source (2 Kings 2:19-22), so "grace" can sweeten the source of a Christian's words. "Let your speech always be with grace, seasoned with salt, that you may know how you ought to answer each one" (Colossians 4:6).
We should choose to have as much "wholesome" language entering our ears and mind as we possibly can. "Apply your heart to instruction, And your ears to words of knowledge" (Proverbs 23:12). In New Testament terms, we must: "Hold fast the pattern of sound words which you have heard from me, in faith and love which are in Christ Jesus" (2 Timothy 1:13).
Other proverbs that emphasis this truth include: "A man has joy by the answer of his mouth, And a word spoken in due season, how good it is!" (Proverbs 15:23); "The heart of the righteous studies how to answer, But the mouth of the wicked pours forth evil" (Proverbs 15:28); "The heart of the wise teaches his mouth, And adds learning to his lips. Pleasant words are like a honeycomb, Sweetness to the soul and health to the bones" (Proverbs 16:23-24); "He who gives a right answer kisses the lips" (Proverbs 24:26); "A word fitly spoken is like apples of gold In settings of silver" (Proverbs 25:11).
"Perverseness" is every deviation or destruction of the absolutes of "love, joy, peace, longsuffering, kindness, goodness, faithfulness, gentleness, self-control. Against such there is no law" (Galatians 5:22-23). If our minds are flooded with "perverseness," it is by choice, becomes self-defeating, but can be overcome. God said Israelites "have corrupted themselves" and had become "A perverse and crooked generation" (Deuteronomy 32:5) who were "Children in whom is no faith" (Deuteronomy 32:20). The Gospel of Jesus Christ commanded "all the house of Israel" to "Repent, and let every one of you be baptized in the name of Jesus Christ for the remission of sins" (Acts 2:36, 38), so that they would "Be saved from this perverse generation" (Acts 2:40). In the generation awash with "media communication," deliver yourself from Generation P(erverse), and "fornication and all uncleanness or covetousness, let it not even be named among you, as is fitting for saints; neither filthiness, nor foolish talking, nor coarse jesting, which are not fitting, but rather giving of thanks" (Ephesians 5:3-4), and "See then that you walk circumspectly, not as fools but as wise, redeeming the time, because the days are evil. Therefore do not be unwise, but understand what the will of the Lord is" (Ephesians 5:15-17).
All Scriptures and comments are based upon the New King James Version, unless otherwise noted.


Emerson College has been named 2015 Private University of the Year by The Washington Center for its ongoing commitment to providing educational opportunities for students in the nation's capital.
TWC is a nonprofit organization that serves hundreds of colleges and universities in the United States and other countries by providing selected students challenging opportunities to work and learn in Washington, D.C., for academic credit.
Emerson's Washington, D.C. Program offers qualified juniors and seniors of any major the opportunity to spend a fall semester in the nation's capital, focusing on the issues, processes, and decisions surrounding government, social advocacy groups, nonprofit organizations, businesses, economics, and more.
Students may enroll for one semester of study, in the fall term only. Accepted students will take 8 credits of internship at a site chosen for their specific goals or interests, and 8 credits of classes.


text:
Praydinae és una subfamília de lepidòpters pertanyent a la família Yponomeutidae. Alguns autors la tracten com una família independent, Praydidae, de Yponomeutoidea.

Gèneres
 Atemelia Herrich-Schäffer, (1853)
 Prays Hübner, [1825]
 Distagmos Herrich-Schäffer, (1853)

Referències

 Sohn, JC, 2012: A new genus and species of Praydidae (Lepidoptera: Yponomeutoidea) from Vietnam. Tinea 22 (2): 120-124.
 Sohn, J.-C. & Alba, M.P., 2014: A New Species of Atemelia (Lepidoptera, Yponomeutoidea, Praydidae) Feeding on the Ornamental Shrub Mahonia (Ranunculales: Berberidaceae) in Chile. Annals of the Entomological Society of America, 107 (2): 339-346.
 Sohn, J.-C. & J.A. Lewis, 2012: Catalogue of the type specimens of Yponomeutoidea (Lepidoptera) in the collection of the United States National Museum of Natural History. Zootaxa 3573: 1-17. Abstract: .

Enllaços externs

 Praydinae at Australian Faunal Directory
 Praydinae at funet

Lepidòpters

redpajama_set_name: RedPajamaWikipedia


text:
Sidymella angulata är en spindelart som först beskrevs av Arthur Urquhart 1885.  Sidymella angulata ingår i släktet Sidymella och familjen krabbspindlar. Inga underarter finns listade i Catalogue of Life.

Källor

Externa länkar

Krabbspindlar
angulata


The constitutional law of July 2008 and the organic law of June 2010 gave citizens the opportunity to refer any economic, social or environmental matter to the ESEC by petition.
Petitions must be written in French and include at least 500,000 signatures by adults of French nationality or lawfully residing in France. Each signature should be accompanied by the person's name and address.
Once the 500,000 signatures have been collected, they must be submitted to the Council President by a single representative. The Bureau will then rule on whether the petition is admissible. The Council will give its verdict at a plenary assembly on the issues raised by approved petitions within one year, along with proposed follow-up.
The opinion will be addressed to the prime minister, the president of the National Assembly, the president of the Senate and the representative who submitted the petition, and will be published in the Official Journal.


text:
Q: How can I find multiple motifs(substring) in a protein sequence(string)? The following script is for finding one motif in protein sequence.
use strict;
use warnings;

my @file_data=();
my $protein_seq='';
my $h= '[VLIM]';
my $s= '[AG]';
my $x= '[ARNDCEQGHILKMFPSTWYV]';
my $regexp = "($h){4}D($x){4}D"; #motif to be searched is hhhhDxxxxD
my @locations=();

@file_data= get_file_data("seq.txt");

$protein_seq= extract_sequence(@file_data);

#searching for a motif hhhhDxxxxD in each protein sequence in the give file

foreach my $line(@file_data){
    if ($motif=~ /$regexp/){
        print "found motif \n\n";
      } else {
        print "not found \n\n";
    }
}
#recording the location/position of motif to be outputed

@locations= match_position($regexp,$seq);
if (@locations){
    print "Searching for motifs $regexp \n";
    print "Catalytic site is at location:\n";
  } else {
    print "motif not found \n\n";
}
exit;

sub get_file_data{
    my ($filename)=@_;
    use strict;
    use warnings;
    my $sequence='';

    foreach my $line(@fasta_file_data){
        if ($line=~ /^\s*(#.*)?|^>/{
            next;
          }
        else {
            $sequence.=$line;
        }
    }
    $sequence=~ s/\s//g;
    return $sequence;
}

sub(match_positions) {
    my ($regexp, $sequence)=@_;
    use strict;
    my @position=();
    while ($sequence=~ /$regexp/ig){
        push (@position, $-[0]);
    }
    return @position;
}

I am not sure how to extend this for finding multiple motifs (in a fixed order i.e motif1, motif2, motif3) in a given file containing a protein sequence.

A: You could simply use alternations (delimited by |) of the sequences. That way each sequence the regex engine can match it will.
/($h{4}D$x{4}D|$x{1,4}A{1,2}$s{2})/

Then you can test this match by looking at $1.

A: If you want to find these motifs in a particular order but perhaps separated somewhat, you could use something like:
/$h{4}D$x{4}D .* $s{4}D$q{4}/x

(/x allows for whitespace in the regex, .* matches zero or more characters)

A: are you JUST looking for substrings? if that's the case a couple of regexes will probably get you where you need to go. but these kinds of problems tend to escalate quickly, most likely in next week's problem set. if the latter is the case, and you're going to need to do comparisons you probably need to start looking into dynamic alignment algorithms, minimum edit distance, viterbi alignment, hmms and the like.
also, if you are dealing with large input files, you might look into pre-compiling your regexes for a nice speed boost,
perl pre-compiled regexes

redpajama_set_name: RedPajamaStackExchange


SANTA MONICA (November 3, 2015) - "Give Where it Really Counts" are the words engraved on Third Street Promenade's Dolphin Change Program plaque, and each year, Downtown Santa Monica, Inc. makes good on this statement. This year,DTSM, Inc issued $10,945 to local nonprofit Step Up On Second during the Westside Shelter & Hunger Coalition's 20th annualCelebrating Success Breakfast at Loews Santa Monica Beach Hotel on Oct. 29. The grant was raised by the Dolphin Change Program, which gives locals an opportunity to help the homeless without giving directly to panhandlers.
This year's recipient Step Up On Second will direct the grant into three strategic efforts supporting those affected by mental illness: member-driven supportive services, employment training and placement opportunities, and permanent supportive housing.
Since its inception in 1993, the Dolphin Change Program has raised $235,000 for social service agencies in the Westside community. Designed by internationally acclaimed naturalist sculptor Peter Erlich, the gentle oceanic swimmer was chosen as the program's key element for its giving and intuitive nature. Dolphin banks located at Third Street Promenade, Santa Monica Pier, Main Street, and the Canyon Charter Business School collect change from street-walking Samaritans year-round. In the past, the program has helped Chrysalis source jobs for the homeless, Turning Point get people off the streets, and CLARE Foundation provide alcoholism and substance abuse victims a compassionate pathway to recovery.
"The greatest part about the program is the support it offers to agencies that have made a real difference in people's lives," says Kathleen Rawson, CEO of DTSM, Inc. "These agencies provide essential support to the most vulnerable in our community to stop the destructive patterns and address the issues that create homelessness."
Downtown Santa Monica, Inc. is a private non-profit 501c(3) that works with the City of Santa Monica to manage services and operations in Downtown Santa Monica that promote economic stability, growth and community life within Downtown Santa Monica. DTSM, Inc. manages the Property-based Business Assessment District (PBAD) that encompasses the area bounded by Ocean Avenue (W), Wilshire Boulevard (N), 7th Court (E) and the Santa Monica Freeway (S). For more info, please visit DowntownSM.com or visit us on Facebook at www.facebook.com/DowntownSantaMonica.


GetHuman-ashstav's next move: go to the Groupon help center.
Groupon doesn't have a customer phone number that is answered by an agent. Instead, GetHuman-ashstav will want to follow the link below to head to their help center. We will check on GetHuman-ashstav later to make sure it's all set or try to help escalate.
I ordered satin sheets on December **st and I did not receive them until january *th and now I don't need them anymore. I also need to be unsubscribed to the stupid hippie butler subscription that I did not sign up for. I received an email from Groupon saying I was subscribed to hippie butler now. It shows I redeemed one of them but I never did and don't know how to use it so I would like it refunded ASAP please. I've reached out numerous times and sent a few different emails. I need help with this ASAP please. You can contact me at **********, if u don't answer please leave a voicemail with a number I can call back to straighten this all out. Thank you!
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Paramedics at South Western Ambulance Service NHS Foundation Trust are reminding people to take care as another yellow weather warning has been issued for the region.
The trust reports it sees an increase in cases of slips, trips and falls during icy conditions as well as an increase of road traffic collisions due to black ice and people driving too fast in the cold weather.
A trust spokesperson said: "If you do need to go out, try and wear the appropriate footwear and warm clothing and make sure you take care and respect the roads.
"It is also time to stock up on any prescription medicines and visit your pharmacy so you can self-medicate. Look in on your vulnerable neighbours and relatives to check they are safe, warm and have enough food.
"NHS guidelines suggest that if you are 65 or over, or have a health condition, such as heart or lung disease it is best to heat your home to at least 18C (65F). Cold weather can be a problem when the temperature drops to below 8C, some people are more at risk of heart attack, stroke, flu, pneumonia, falls and hypothermia.


text:
Vitamin D Status and COVID-19 Clinical Outcomes in Hospitalized Patients.
Vitamin D Status and COVID-19 Clinical Outcomes in Hospitalized Patients
In this retrospective chart review there was no association between vitamin D levels within 1 year of hospital admission for COVID-19 and clinical outcomes.
Subjects with 25(OH)D < 20 ng/mL (n = 35) did not differ from those with 25(OH)D ≥ 20 ng/mL in terms of age, sex, race, BMI, or comorbidities.
Low 25(OH)D tended to be associated with younger age and lower frequency of preexisting pulmonary disease.
There were no significant between-group differences in any outcome. Results were similar in those ≥50 years, in male/female-only cohorts, and when differing 25(OH)D thresholds were used (<15 ng/mL and <30 ng/mL). There was no relationship between 25(OH)D as a continuous variable and any outcome, even after controlling for age and pulmonary disease.
Data on 93 adult patients with a positive PCR test for COVID-19 admitted to Columbia University Irving Medical Center (CUIMC) in New York City were extracted from the electronic medical record and reviewed for an available 25(OH)D level within 365 days of hospitalization.
This was a single center study of just 93 patients, had more of the patients had vitamin D levels available the data would be stronger.
The vitamin D values could have been measured within one year of admission and therefore may not be actual levels at the time of admission.
A retrospective chart review does not allow for strong conclusions.
These preliminary data do not support a relationship between prehospitalization vitamin D status and COVID-19 clinical outcomes.
This was a retrospective medical record review of 700 COVID-19 positive adults admitted to the hospital, of those 93 had vitamin D levels measured within a year prior to admission.
Vitamin D deficiency was defined as serum 25(OH)D level less than 20 ng/mL.
Total calcium level, albumin, alkaline phosphatase, BUN, and creatinine obtained within 30 days of the serum 25(OH)D level were also extracted from the records.
The primary outcomes of interest were discharge status (deceased/discharged to hospice, discharged (not death), still admitted), intubation status (ever, never), renal replacement during hospitalization (including hemodialysis and continuous veno-venous hemofiltration), and duration of stay.
For patients who were intubated during the admission, the duration of intubation was also calculated.
Other variables of interest were age, sex assigned at birth, BMI, race, and ICD-defined comorbidities, including hypertension, diabetes, chronic kidney disease, and any pulmonary disease.
Szeto B, Zucker JE, LaSota ED, Rubin MR, Walker MD, Yin MT, Cohen A. Vitamin D Status and COVID-19 Clinical Outcomes in Hospitalized Patients. Endocr Res. 2021 Feb-May;46(2):66-73. doi: 10.1080/07435800.2020.1867162. Epub 2020 Dec 30. PMID: 33380209; PMCID: PMC7784779.


text:
$(document).ready(function(){
    $( "body" ).delegate( "button.check_url_valid", "click", function() {
        var btn = this;
        var url = $(this).parent().parent().find('input:first').val();
        var type = '';
        var attr = $(this).attr('url-type');
        var thisID = $(this).attr('id');
        if (attr) {
            type = $(btn).attr('url-type');
        } else {
            if (thisID == "prototype_test") {
                type = "http";
            }
            else {
                type = $.parseJSON($("#endpoint_config").val())['endpoint_type']
            }
        }
        $(btn).parent().parent().find('.url_validate_label').remove();
        $(btn).addClass("loadingButton-small");
        $(btn).val(i18n.t('validationMsgs.validating'));

        if (url == '') {
            $(btn).parent().parent().after(' '+ i18n.t('validationMsgs.missingUrl')+'');
            var toFade = $(btn).parent().parent().parent().find('.url_validate_label');
            $(btn).removeClass("loadingButton-small");
            $(btn).val(i18n.t('validationMsgs.testUri'));
            var foo = setTimeout(function(){$(toFade).hide()},3000);
            return;
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        if (!type) {
            type = "";
        }
        jagg.post("/site/blocks/item-add/ajax/add.jag", { action:"isURLValid", type:type,url:url },
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                                    $(btn).parent().parent().after(' ' + i18n.t('validationMsgs.invalid') + '');
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' + result.response.response + '');
                                    } else {
                                        $(btn).parent().parent().after(' ' + i18n.t('validationMsgs.invalid') + '
' + result.response.statusCode + ' - ' + result.response.reasonPhrase + '');
                                    }
                              }

                          }
                          var toFade = $(btn).parent().parent().find('.url_validate_label');
                          var foo = setTimeout(function() {
                                $(toFade).hide();
                          }, 3000);
                      }
                      $(btn).removeClass("loadingButton-small");
                      $(btn).val(i18n.t('validationMsgs.testUri'));
                  }, "json");

    });
});

redpajama_set_name: RedPajamaGithub