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This is a Campbell 412 HO DeWitt's Depository. This craftsman kit consists of scale lumber (sugar pine) and is ready for paint and weatherizing. Complete instructions, drawings, and templates are included.
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The acquisition of multidrug resistance by Plasmodium falciparum underscores the need to understand the underlying molecular mechanisms so as to counter their impact on malaria control. For the many antimalarials whose mode of action relates to inhibition of heme detoxification inside infected erythrocytes, the digestive vacuole transporters PfCRT and PfMDR1 constitute primary resistance determinants.
Using gene expression microarrays over the course of the parasite intra-erythrocytic developmental cycle, we compared the transcriptomic profiles between P. falciparum strains displaying mutant or wild-type pfcrt or varying in pfcrt or pfmdr1 expression levels. To account for differences in the time of sampling, we developed a computational method termed Hypergeometric Analysis of Time Series, which combines Fast Fourier Transform with a modified Gene Set Enrichment Analysis. Our analysis revealed coordinated changes in genes involved in protein catabolism, translation initiation and DNA/RNA metabolism. We also observed differential expression of genes with a role in transport or coding for components of the digestive vacuole. Interestingly, a global comparison of all profiled transcriptomes uncovered a tight correlation between the transcript levels of pfcrt and pfmdr1, extending to dozens of other genes, suggesting an intricate regulatory balance in order to maintain optimal physiological processes.
This study provides insight into the mechanisms by which P. falciparum adjusts to the acquisition of mutations or gene amplification in key transporter loci that mediate drug resistance. Our results implicate several biological pathways that may be differentially regulated to compensate for impaired transporter function and alterations in parasite vacuole physiology.
With an estimated 214 million clinical cases and 438,000 deaths in 2015  malaria remains one of the deadliest infectious diseases. Clinical manifestations are due to successive rounds of Plasmodium parasite invasion, replication within host erythrocytes, egress and re-invasion. Present efforts to reduce mortality and morbidity focus on the use of curative and preventive drug treatments as well as on vector control strategies . Those include first-line artemisinin-based combination therapies (ACTs) that pair a highly potent artemisinin derivative with a longer-acting partner drug . ACTs currently constitute the best regimens to treat 4-aminoquinoline- and antifolate-resistant Plasmodium falciparum parasites that have swept across the world. While 20 years were necessary for chloroquine (CQ) resistance to spread from its origins in Southeast Asia to Africa , resistance to the antifolate combination of sulfadoxine-pyrimethamine disseminated at a much faster pace . The recent emergence of delayed parasite clearance following artesunate or ACT administration in Western Cambodia and Thailand [6–8] reemphasizes the need to develop therapeutic strategies to treat drug-resistant P. falciparum malaria and define the molecular basis of resistance.
Two key genetic determinants of P. falciparum resistance to antimalarial compounds are pfcrt  and pfmdr1 [10, 11], which encode transporter proteins that localize to the digestive vacuole membrane of intra-erythrocytic parasites. While their native biological functions remain unknown, the inability to genetically disrupt pfcrt or pfmdr1 provides evidence for their essentiality [12, 13]. Laboratory and field-based studies have demonstrated that PfCRT mutations constitute the primary determinant of in vitro CQ resistance and are associated with a substantially increased risk of CQ treatment failure in patients [14–16]. Studies with transgenic parasites or heterologous expression systems have attributed PfCRT-mediated CQ resistance to drug efflux out of the low-pH digestive vacuole, where CQ becomes diprotonated, concentrates up to 1,000-fold and acts by binding to heme products and preventing heme detoxification [17–20]. PfCRT mutations can also mediate cross-resistance to amodiaquine and increase susceptibility to lumefantrine, mefloquine and artesunate [15, 21, 22]. pfmdr1 mutations as well as copy number variations have also been shown to impact P. falciparum susceptibility to several antimalarials [13, 22–26] presumably via drug transport across the digestive vacuole .
For PfCRT and PfMDR1, acquired resistance mutations, or in the case of pfmdr1 gene amplifications, are thought to generally impart a fitness cost [28–31], presumably via altering their endogenous transport properties to the detriment of normal parasite development and replication. In endemic settings, this fitness cost manifests as a reduced prevalence of mutant pfcrt or pfmdr1 following the release of selective drug pressure [32–36]. However, additional mutations elsewhere in an organism can compensate for the cost of acquiring resistance [37, 38] and restore fitness, as shown for P. falciparum resistance to antifolates [39–41]. Furthermore, the long-term propagation of drug-resistant strains even in the absence of selective pressure suggests adaptive mechanisms that counteract the detrimental effects of resistance-conferring genetic changes .
Intriguingly, the question of how parasites transcriptionally adjust in the long-term to the acquisition of mutations or gene amplifications in drug resistance transporters has scarcely been addressed. An earlier comparison of the transcriptional profiles of pfcrt mutant strains generated by CQ selection from a drug-sensitive parasite line suggested a limited number of changes in the parasite transcriptome . That study, however, was restricted to a single time point of P. falciparum development within the host erythrocytes and as such it could not correct for temporal differences in the intra-erythrocytic developmental cycle (IDC) between parasite lines. Indeed, while transcriptome studies have suggested an inability of the parasite to transcriptionally respond to short-term drug pressure [44, 45], conceivably parasites may be able to evolve over multiple generations to states of optimal growth that adjust for physiological impacts of altered sequence or expression of drug/solute transporters. This is a testable hypothesis for PfCRT and PfMDR1, whose expression kinetics are closely tied to the formation of the parasite digestive vacuole during the trophozoite stage. Changes in the transcriptome profile of these mutant lines can be assessed in comparison with the carefully documented timeline of tightly regulated gene expression patterns that characterize the P. falciparum IDC [46–48]. These expression patterns underpin the biological processes of intracellular parasite development, replication and egress with accompanying morphological changes as parasites progress from rings to trophozoites to schizonts.
Here, we investigated how parasites adjusted transcriptionally to genetic modification of pfcrt or pfmdr1, resulting in altered drug resistance phenotypes following long-term culture. Using DNA microarrays, we conducted a genome-wide transcriptional profiling of genetically modified isogenic P. falciparum strains with either distinct PfCRT haplotypes or pfmdr1 functional copy numbers throughout their 48-hour IDC. These previously engineered lines either display a loss of CQ resistance following the removal of the K76T mutation  (used epidemiologically as the primary molecular marker of CQ resistance) or show increased susceptibility to multiple antimalarial drugs (including mefloquine, lumefantrine, quinine and artemisinin) following genetic disruption of one of the two pfmdr1 copies present in the multidrug resistant FCB line . To analyze the data we developed a computational method that combines Fast Fourier Transform (FFT) and Gene Set Enrichment Analysis (GSEA) to correct for temporal differences between samples and compare microarray data between time series. We also used a reference pool of 11 profiled parasite lines to distinguish between stochastically variant genes and gene expression differences of biological significance, including those of subtle amplitude. Our results revealed a striking and previously unreported correlation between the expression levels of pfcrt and pfmdr1, and identified several pathways whose regulation was altered in response to changes in either gene, reinforcing the hypothesis of their functional interplay and the existence of coordinated functional networks.
To assess the genomic impact of P. falciparum acquiring genetic changes in transporters that mediate antimalarial drug resistance, we profiled the transcriptome of multiple isogenic lines characterized by altered pfcrt and pfmdr1 gene expression levels and/or corresponding protein haplotypes (Table 1). We also transcriptionally profiled the parental strains and performed both gene-specific and combined comparative analyses of the data sets.
The first data set (focusing on pfmdr1) included FCB pfmdr1_3′KD , which was engineered via single-site crossover-based disruption of one of the two pfmdr1 copies within the duplicated 100-kb genomic region of the multidrug-resistant parasite strain FCB [26, 49]. This resulted in a 50 % decrease in pfmdr1 gene expression and a reduction of approximately 40 % in its protein level (Additional file 1: Figure S1A). In addition to FCB pfmdr1_3′KD and its parental line FCB, our transcriptome analysis included 106/1, a parasite strain that genetically is nearly identical to FCB . FCB and FCB pfmdr1_3′KD display the same PfCRT haplotype (including the K76T mutation that is required for CQ resistance), whereas 106/1 has a key difference at position 76 that expresses a lysine (K) and confers CQ susceptibility [9, 51].
The second data set (pfcrt) included 7G8 pfcrt_T76K and 7G8 pfcrt_CTL parasite lines that were generated by allelic exchange via single crossover recombination targeting codon 76 of the mutant pfcrt allele in the CQ-resistant strain 7G8 . The encoded threonine residue was back mutated to the wild-type lysine in 7G8 pfcrt_T76K , resulting in a complete loss of CQ resistance, whereas it remained identical to 7G8 in the recombinant control 7G8 pfcrt_CTL . Additionally, the two recombinant lines are characterized by a two-fold reduction in pfcrt mRNA and protein expression, allowing us to assess the impact of both the acquisition/loss of a point mutation and alteration in gene expression (Additional file 1: Figure S1B). Of note, 7G8 pfcrt_CTL is only moderately CQ-resistant, with a mean CQ IC50 value (109 nM) that is close to half that of the parental 7G8 line (251 nM) by virtue of the reduced PfCRT expression .
To conduct our comparative analysis, tightly synchronous parasites were cultured for the length of a complete IDC (~48 h). RNA was extracted from samples collected every 6 h for 48 h and assayed on a 70-mer DNA microarray, representing more than 5,350 P. falciparum genes . Alignment of each profiled time series to the transcriptome of the reference strain 3D7 showed small but significant differences in the IDC duration. Indeed, the transgenic strains displayed a shorter life cycle than their respective parents (Fig. 1a).
Given the resulting slight time shifts between the time series, we developed a computational method to align the microarray expression data and to ensure that our comparative analysis would not be skewed by temporal differences in the developmental stage of the different parasite lines at the time of harvesting. We used FFT to impute gene expression levels at 376 unassayed time points to obtain a 7.5-minute resolution of each time course (384 time points in total; see Methods, Additional file 2: Table S1). To validate this approach, we first performed a simulation using published transcriptome data sets that constituted of hourly time course data for the strains HB3, Dd2 and 3D7 . For each strain we selected 8 time points spread 6 h apart, from which we generated a curve of 376 imputed points. Using Pearson correlation values for gene expression, we then compared each actual hourly time point to the imputed curve to identify the closest matching time point. We observed that actual time points from the original time courses matched imputed time points that were fairly evenly spread along the imputation-derived curve, with correlations of average 83–91 % across the range of time points for each strain. This suggests that the imputation method can work on datasets that comprise only eight time points, such as ours.
Using our imputed data sets, we then applied a dynamic time warping/Pearson Correlation Coefficient (PCC) method to determine for each profiled parasite line the optimal post-invasion time point (corresponding to the first sampling time point) along the IDC (see Methods). All time courses were then realigned to the 3D7 transcriptome and to each other when performing pairwise comparisons (Additional file 3: Figure S2).
Phaseograms representing the transcriptome of each strain were assembled by hierarchically clustering the mean-centered log2 expression ratios of the genes that passed the different filters (~3,500 genes), according to their phase of expression calculated as described above. Transcriptomes of all strains reproduced the cascade of expression typical of P. falciparum, in which genes are expressed in a highly periodic mode  (Fig. 1b, c). Furthermore, all profiled strains demonstrated excellent synchronicity, with tight clustering of the expression data as observed using Principal Component Analysis (PCA), thereby validating the alignment of the time series (Fig. 1d).
To identify genes that were differentially expressed between parasite time series within each data set, steady state transcript levels were analyzed across the IDC for each profiled strain and compared to each other. We computed gene expression fold differences for each pairwise comparison as the difference between the areas under the curves, which were extracted from absolute temporal gene expression plots derived from the full 384 time point data.
We then generated chromosome maps in which fold changes in expression were plotted as a function of the chromosomal position of each gene. These maps revealed clusters of differential expression in telomeric regions of several chromosomes, possibly indicating hypervariable transcription of genes within these regions, as previously reported  (Additional file 4: Figure S3). Surprisingly, an entire set of genes all located on chromosome 5 and overlapping with the 100-kb amplicon containing pfmdr1 (PF3D7_0523000) appeared to be differentially expressed between FCB and both FCB pfmdr1_3′KD and 106/1 (Fig. 2a, b). All genes within this region except PF3D7_0521300, PF3D7_0521800, PF3D7_0522900, and PF3D7_0523100 were expressed two- to four-fold less in FCB pfmdr1_3′KD and 106/1 as compared to FCB (Fig. 2a, b; Additional file 5: Table S2). Interestingly, global gene expression profiles within the amplicon region on chromosome 5 were extremely similar between FCB pfmdr1_3′KD and 106/1 (Fig. 2a, b). Given the overlap with genes that constitute the amplicon in the FCB strain (Additional file 6: Figure S4), we performed quantitative real time PCR on parasite genomic DNA to assess whether the observed transcriptional differences between FCB and both FCB pfmdr1_3′KD and 106/1 originated from different copy numbers within the pfmdr1 region on chromosome 5. Quantitative PCR results indicated that the FCB pfmdr1_3′KD strain has a single copy of the genes that are found duplicated in FCB, including pfmdr1. Furthermore, although our microarray data showed a similar down-regulation of these genes in 106/1, the parasite strain harbored two physical copies of these. These results with 106/1 indicate a different mechanism for reduced gene expression in that chromosomal region, potentially involving silencing of one of the two amplicons (Additional file 7: Table S3).
The same chromosomal mapping of gene expression fold changes revealed the down-regulation of a cluster of genes on chromosome 2 in the back-mutant strain 7G8 pfcrt_T76K in comparison to the background strain 7G8 (Fig. 2c; Additional file 8: Table S4). This down-regulation was specific to the back-mutant parasites, as no gene expression difference was observed in that region when comparing the transgenic control strain 7G8 pfcrt_CTL to 7G8 (Fig. 2d). Interestingly, many of the affected genes encode proteins involved in parasite invasion of host erythrocytes, including Merozoite Surface Proteins (PF3D7_0206800 and PF3D7_0207000) and Serine Repeat Antigen (SERA) gene products (PF3D7_0207400, PF3D7_0207500, PF3D7_0207600, PF3D7_0207700, and PF3D7_0207800) (Fig. 2c, Additional file 8: Table S4).
To assess the transcriptional variations between the profiled strains, we performed gene expression and gene set enrichment analyses, considering both absolute and normalized gene expression fold changes (Methods). We also conducted pairwise comparisons of the transcriptome data sets for three developmental stage windows, corresponding to rings (12 to 16 h post-invasion), trophozoites (24 to 28 h post-invasion) and schizonts (32 to 36 h post-invasion. These comparisons enabled us to detect changes in genes/gene sets within a short window of expression or genes/gene sets whose peak of expression occurred at different times between related parasite lines (Methods; Additional file 9: Figure S5). We observed that more than 70 % of all genes peaked within the same 8-hour window in the pfmdr1 dataset while about 80 % of the genes peaked within the same 5-hour window in the pfcrt dataset (Additional file 10: Table S5). Overall, the great majority of the observed changes was driven by changes in gene expression levels as opposed to altered timing of expression.
Analysis of the pfmdr1 data set (FCB vs. FCB pfmdr1_3′KD and FCB vs. 106/1) showed that many of the genes with significant differences in expression level encoded proteins of unknown function. This analysis also revealed decreased expression of multiple genes involved in RNA and protein metabolism upon deamplification of the pfmdr1-containing amplicon region on chromosome 5 (Fig. 3a, b). Among these were genes coding for RNA-binding proteins, splicing/exosome elements and protein chaperones and modifiers (Additional file 11: Table S6A,B). In contrast, numerous genes encoding ribosomal protein subunits, or involved in RNA translation initiation or protein turnover showed a more than two-fold higher expression level in both strains with lower pfmdr1 expression (Fig. 3c, d). Furthermore, genes mainly involved in transcriptional control, including several elements of the basal transcription machinery, were 2 to 3.5-fold up-regulated in FCB pfmdr1_3′KD and 106/1 in comparison to FCB. For instance, those encoding a putative RNA polymerase II (PF3D7_1027400), and the AP2-containing transcription factors PF3D7_0730300 and PF3D7_0613800 [54, 55] displayed higher expression in FCB pfmdr1_3′KD (Additional file 11: Tables S6C). Genes encoding chromatin constituents such as the components of the nucleosome core histone H3 (PF3D7_0610400) and the histone variant H2B (PF3D7_0714000) were 2 to 2.5–fold less expressed in FCB in comparison to 106/1 (Additional file 11: Table S6D). These results suggest an alteration of the global transcriptional and translational activities in both strains with reduced pfmdr1 expression in comparison to FCB.
Multiple genes implicated in cellular metabolism, parasite remodeling and invasion also displayed higher transcript levels in FCB pfmdr1_3′KD and 106/1 compared to FCB (Fig. 3c, d, Additional file 11: Table S6C,D). These observations were confirmed by applying a statistical approach that we termed Hypergeometric Analysis of Time Series (HATS), which combined Fast Fourier Transform and GSEA, to further assess the functional significance of the variations in gene expression observed between the parasite strains (see Methods). HATS, although based on GSEA, imputes a gene set enrichment score from the hypergeometric distribution of all gene sets calculated following 1,000 random permutations, instead of using a weighted Kolmogorov-Smirnov-like test . HATS demonstrated that genes more highly expressed in FCB in comparison to FCB pfmdr1_3′KD and 106/1 were enriched mainly for metabolic and enzymatic activities such as purine/pyrimidine and pyruvate/glutamate metabolic pathways (Fig. 4a, b). In contrast, several genes and gene sets associated with transcription regulation, translation initiation including ribosome biogenesis, and proteasome-mediated protein catabolism/turnover, were depleted in FCB in comparison to both strains with lower pfmdr1 expression (Fig. 4c, d). Stage-enrichment analysis (Methods) revealed that these differences were detectable mainly during the trophozoite and schizont stages (Fig. 4e, f, Additional files 12 and 13: Tables S7 and S8 respectively). Some of these alterations, including those affecting protein synthesis and protein turnover via proteasome degradation have also been observed in the transcriptional profiles of artemisinin-resistant patient isolates carrying causal mutations in the kelch gene K13, suggesting that these pathways might contribute to general mechanisms adopted by multi-drug resistant parasites .
Further stage-specific analysis showed that gene categories comprising transporters or components localized to the parasite digestive vacuole were significantly enriched (>2-fold) in FCB in comparison to FCB pfmdr1_3′KD in mature asexual blood stages, i.e. when the digestive vacuole is functioning. These genes included two V-type ATPases (PF3D7_1354400, PF3D7_0519200), an AAA family ATPase (PF3D7_0707400), an ABC transporter (PF3D7_0810200), and drug/metabolite and amino acid transporters (PF3D7_1428200 and PF3D7_1132500), (Fig. 4e, Additional file 12: Table S7A,C,G). These genes sometimes also displayed a higher expression in FCB in comparison to 106/1 (Fig. 4f, Additional file 13: Table S8A).
Overall, the global transcriptional response to the deamplification of the pfmdr1-containing amplicon was highly similar between FCB pfmdr1_3′KD and 106/1. For instance, expression analysis of individual genes revealed that both FCB pfmdr1_3′KD and 106/1 strains in comparison to FCB showed a 2- to 3-fold down-regulation of the genes coding for an ATP-dependent RNA helicase (PF3D7_0521700) and the H2 ribonuclease (PF3D7_0623900) (Additional file 11: Table S6E). These two genes were among the very few that were differentially expressed between the strains across all time points throughout the IDC, as demonstrated by stage-specific analysis (Additional files 12 and 13: Tables S7K and S8K respectively). HATS also identified several gene sets that were enriched or depleted in both FCB pfmdr1_3′KD and 106/1 in comparison to FCB, which included parasite transporters, components of the proteasome degradation machinery and ribosome constituents (Additional file 11: Table S6G,H). Overall, we observed an overlap of greater than 50 % in the gene sets enriched or depleted in both FCB pfmdr1_3′KD and 106/1 in comparison to FCB (Fig. 4g, h, Additional file 11: Table S6E,F). Interestingly, we also detected enrichment for gene sets associated with mitochondrial activities such as energy production and electron transport in the FCB versus 106/1 pairwise comparison, but not when comparing FCB with FCB pfmdr1_3′KD (Fig. 4a, b). These gene sets in addition to those related to aminoacyl-tRNA biosynthesis, redox regulation or associated with the parasite digestive vacuole were differentially enriched between both pfmdr1 knock-down strains (Additional file 14: Figure S6). The contrasting nature of the PfCRT haplotypes between the two strains might account for these differences. This aspect was further addressed when analyzing the pfcrt data set.
We applied analytical approaches similar to those described above to the pfcrt data set (Additional file 15: Table S9) and performed pairwise comparisons of 7G8, 7G8 pfcrt_CTL and 7G8 pfcrt_T76K transcriptomes. Expression analysis for individual genes showed that several genes encoding proteins involved in transcriptional regulation, such as histone deacetylases (PF3D7_1472200) and specific transcription factors (PF3D7_1222400, PF3D7_1222600) from the ApiAP2 family were globally less expressed in the transgenic back-mutant 7G8 pfcrt_T76K and control 7G8 pfcrt_CTL strains in comparison to the parental line 7G8 (Fig. 5a, b, Additional file 15: Table S9A,B). Additionally, genes involved in the maintenance of mitochondrial integrity and activity, such as the mitochondrial import inner membrane translocase subunits TIM13 and TIM44 (PF3D7_1242900 and PF3D7_1125400, respectively) and one of the cytochrome c1 precursors (PF3D7_1462700), showed 2- to 3.5-fold lower transcript levels in 7G8 pfcrt_CTL and 7G8 pfcrt_T76K in comparison to 7G8 (Fig. 5a, b, Additional file 15: Table S9A,B). Stage-specific analysis revealed that down-regulation of the genes encoding the TIM subunits was most apparent during the schizont stage for both transgenic strains (Additional files 16 and 17: Tables S10E and S11E respectively), which shared many of the genes displaying a decreased expression when compared to 7G8 (Fig. 5c, Additional file 15: Table S9C). On the other hand PF3D7_0609400, which encodes a cardiolipin synthase equally central to the mitochondrial inner membrane, was among the limited number of genes with higher transcript levels in both transgenic strains (Fig. 5c), particularly at the trophozoite stage (Additional files 16 and 17: Tables S10D and S11D). These observations suggest that decreased pfcrt expression, regardless of its haplotype in the 7G8 background, might affect mitochondrial integrity and functions.
When examining the pfcrt-modified transgenic strains, we observed increased expression in several genes encoding putative proteins involved in protein folding, trafficking and turnover (Fig. 5d, e). These include heat shock and DnaJ proteins, which were on average 2-fold more highly expressed in comparison to 7G8 (Additional file 15: Table S9D-F). Increased expression was also observed with pfhrp3 (PF3D7_1372200), whose product is hypothesized to be involved in hemozoin formation in the parasite digestive vacuole . These observations were confirmed by HATS (Fig. 6), which provided evidence for higher expression of genes in the categories of chaperones and protein catabolism in both 7G8 pfcrt_CTL and 7G8 pfcrt_T76K (Fig. 6a, b). Interestingly, there was a substantial overlap between the gene sets depleted in 7G8 in comparison to either 7G8 pfcrt_CTL or 7G8 pfcrt_T76K (Fig. 6c). Altogether these results suggest that pfcrt transcript levels can influence both parasite-specific processes and general biological pathways irrespective of this transporter's drug resistance phenotype (see Discussion).
Other genes whose expression was higher in the transgenic 7G8 strains in comparison to the parental line were mostly involved in parasite invasion and encoded merozoite surface proteins as well as glideosome and rhoptry components (Figs. 5d, e, 6a, b). Interestingly, these effects were accentuated in the back-mutant 7G8 pfcrt_T76K at both the individual gene as well as gene set levels, during most of the parasite IDC (Fig. 7a-e, Additional files 15 and 16: Table S9 and S10 respectively).
We also compared the transcriptional profiles of 7G8 pfcrt_T76K and 7G8 pfcrt_CTL to assess the impact of the CQ resistance phenotype defined by PfCRT K76T. Genes differentially expressed between these lines were enriched for functions associated with DNA/RNA metabolism and protein catabolism (Fig. 7a, b). For instance, multiple genes encoding proteins possibly involved in DNA replication, such as mini-chromosome maintenance proteins (PF3D7_1355100, PF3D7_1417800), DNA helicases (PF3D7_0918600, PF3D7_1227100, PF3D7_1010200), a nucleotide excision repair enzyme (PF3D7_0710400) and the DNA polymerase α subunit (PF3D7_1463300) were ~2-fold more highly transcribed in 7G8 pfcrt_CTL (Fig. 7a, Additional file 15: Table S9G). Higher expression of some of these genes was observed throughout the IDC as shown by stage-specific analysis (Additional file 18: Table S12). Transcripts involved in RNA processing such as components of the exosome complex (PF3D7_0720000, PF3D7_0209200) showed ~1.5 to 3-fold higher levels in 7G8 pfcrt_CTL , mainly during the ring and trophozoite stages (Additional file 18: Table S12A,C,G). These observations were confirmed by HATS analysis (Fig. 7c, d).
Expression analysis of individual genes and gene sets indicated that genes involved in translation and protein stability, trafficking and turnover, such as those encoding ribosomal subunits and protein chaperones, respectively were also more highly transcribed in the transgenic control line 7G8 pfcrt_CTL (Fig. 7a, c, Additional file 15: Table S9G). Stage-specific analysis demonstrated that higher expression of some of these genes was detected throughout the entire IDC (Additional file 18: Table S12K). Furthermore, HATS revealed that the categories of amino-acyl-tRNA ligases and synthetases were depleted in the back-mutant strain in comparison to both parental and control lines (Figs. 6b and 7c), although several individual genes within these categories, including some encoding a glutamate-tRNA ligase (PF3D7_1349200 and PF3D7_1357200), displayed higher expression in 7G8 pfcrt_T76K at either ring or schizont stages (Additional file 18: Table S12B,F). Interestingly, HATS showed that gene sets linked to tRNA aminoacyl biosynthesis were also enriched in 7G8 pfcrt_CTL in comparison to the parental strain (Fig. 6b). Several tRNA-ligases (PF3D7_0509600, PF3D7_1349200) and ribosyltransferases (PF3D7_1434100) also displayed higher transcript levels in a stage-specific manner (Additional file 16: Table S10B,J), suggesting that translation may also be affected when mutant pfcrt expression is lowered (Additional file 19: Figure S7). Pathways linked to proteasome-mediated degradation and ribosome biogenesis were among the multiple genes and gene sets depleted in both control and parental strains in comparison to the back-mutant 7G8 pfcrt_T76K (Figs. 5e, 6b and 7b, d, Additional files 15: Tables S9K,L). Altogether these observations indicate that the acquisition of drug-resistance mutations in pfcrt may impact protein synthesis and turnover pathways.
Expression analysis for individual genes identified several transporters in addition to pfcrt that were differentially expressed between the two transgenic strains (Fig. 7a, b, Additional file 15: Table S9I,J), mostly in a stage-specific manner (Additional file 18: Table S12). This finding suggests a possible crosstalk between pfcrt and other transporters. For example, ~2-fold higher transcript levels of the ABC transporter PF3D7_0813700 and the folate transporter FT1 (PF3D7_0828600) were detected in 7G8 pfcrt_CTL (Additional file 18: Table S12A,G), while genes encoding amino acid (PF3D7_0629500) and ABC transporters (PF3D7_1229100 and PF3D7_1352100) were ~1.5-fold more expressed in the back-mutant during either the ring or trophozoite stages (Additional file 18: Table S12D,F).
Our comparative analysis of the transcriptome profiles of the FCB- and 7G8-derived parasite lines demonstrated that mutations and/or altered transcript levels in pfmdr1 or pfcrt impacted the expression of similar gene categories. We therefore assessed whether a transcriptional association between pfcrt and pfmdr1 might account for the similarities we observed between the transcriptome data sets.
Using the publicly available transcriptome data sets of the P. falciparum strains 3D7, Dd2, and HB3 [46, 48] that we added to our own data sets, we first performed an expression-based hierarchical clustering of the data, using PCC distances between gene pairs computed across all possible pairwise comparisons of transcriptomic profiles. pfcrt and pfmdr1 ranked closely to each other, and formed two distinguishable clusters with high scores for gene pair correlations (Fig. 8a). One of these clusters included a V-type ATPase localized in the parasite digestive vacuole (PF3D7_1354400, PCC ~0.7–0.8), a DnaJ chaperone protein (PF3D7_1307200, PCC ~0.7–0.8) and a transcription factor of the ApiAP2 family (PF3D7_0516800) (Additional file 20: Table S13). The other cluster contained the RNA Polymerase I responsible for rRNA transcription (PF3D7_1134700, PCC 0.8) (Additional file 21: Table S14). Interestingly, the two clusters also contained PF3D7_0823300 that encodes the histone acetyltransferase GCN5 and PF3D7_0303700, a gene involved in lipoamide synthesis, which is in concordance with a recent study reporting the co-expression of pfcrt with lipoamide synthesis genes . Altogether these observations suggest an association between pfmdr1 and pfcrt expression and that of genes with specialized functions in the digestive vacuole, in addition to genes having more general functions such as rRNA transcription and protein translation.
To further investigate the transcriptional association between pfcrt and pfmdr1, we conducted an additional hierarchical clustering of gene expression, this time, by ordering genes based on the level of correlation they displayed with either pfcrt or pfmdr1 expression (Additional file 22: Figure S8). While more than a hundred genes showed a high transcriptional correlation (PCC >0.7) with each gene, we also detected a large number of genes that were strongly anti-correlated (PCC <−0.7) (Additional file 23: Table S15). Interestingly, many genes (~70 %) with strong positive or negative transcriptional correlation with pfcrt were additionally strongly correlated with pfmdr1 expression (Fig. 8b). A dozen of those genes encode transporters, components of the digestive vacuole, or redox enzymes (Table 2). Others were linked to DNA/RNA-dependent processes, translation, and protein trafficking (such as components of the PTEX parasite translocon of exported proteins) (Additional file 24: Table S16A). On the other hand, several genes implicated in either RNA processing or protein catabolism, such as those encoding splicing factors and elements of the proteasome complex, respectively, showed a strong anti-correlation with both pfcrt and pfmdr1 expression (Table 2, Additional file 24: Table S16B). These findings suggest the existence of a transcriptional network that encompasses both genes and evoke a possible functional association between these two drug resistance transporters.
Studies have previously defined the malaria parasite transcriptome as hard-wired, capable only of very limited changes at the transcript level in response to specific metabolic challenges [43, 44, 60]. Nevertheless, more recent studies have predicted gene functions and networks using genome-wide transcriptional profiling of P. falciparum growth perturbations, and have provided evidence that the parasite can display transcriptional changes even if of low amplitude in response to chemical challenges . In the present study, we have addressed how variant forms of two primary determinants of parasite drug susceptibility can affect the asexual blood stage transcriptome. This focused on a comparative analysis of the transcriptome profiles of P. falciparum parasite lines engineered to display distinct levels of expression and/or mutation in the genes coding for the drug resistance transporters PfCRT and PfMDR1. Genome-wide analysis of the transcriptomes was performed both at the gene and gene set levels using computational approaches that identified and excluded transcriptional differences due to stochastic expression. This enabled us to define a transcriptional baseline and detect genes whose expression significantly diverged, implicating them as candidate factors in a parasite transcriptional adaptation to the pfcrt and pfmdr1 changes that were genetically introduced.
Among the ~5,500 coding genes in P. falciparum genome, we surveyed the expression changes for a set of 3,000 to 3,500 genes for which the microarray data passed quality inspection across at least 6 of the 8 time points in the series. It is therefore possible that we missed additional genes with differential transcriptional profiles. Despite this limitation, we found that genetic modifications of the loci coding for these two digestive vacuole transporters notably affect the transcription of genes coding for RNA-binding proteins, transcription factors, elements of the translation initiation and elongation machinery, and components of both the spliceosome and proteasome complexes. This observation was particularly striking in the pfmdr1 data set that included the FCB isogenic strain, FCB pfmdr1_3′KD . Deamplification of a large region on chromosome 5 that encompassed pfmdr1 led to the increased expression of genes involved in translation and reduced levels of transcripts implicated in protein catabolism and modifications. This represented in total more than 10 % of the global expression changes and is concordant with expression Quantitative Trait Loci analyses that previously demonstrated an association between the pfmdr1-containing amplicon and both post-translational modifications and protein catabolic processes . In agreement with that study , we identified a large number of genes involved in proteasome-mediated degradation and protein ubiquitination that display differential expression between FCB and FCB pfmdr1_3′KD . This suggests that amplification of the pfmdr1-containing amplicon can be accompanied by a global reduction of DNA and RNA metabolic activities, and increased protein catabolism.
Our study also identified mechanisms of transcriptional down-regulation across the pfmdr1 amplicon in both 106/1 (that carries two physical copies) and FCB pfmdr1_3′KD (whose second pfmdr1 copy had been disrupted, and for which the expression of adjacent genes also became downregulated). Earlier studies of Thai field isolates had shown considerable variability in both pfmdr1 copy number and amplicon size, which would impact expression levels of both pfmdr1 and its neighboring genes . Our data highlight the need to account for expression when assessing the association between pfmdr1 copy number and drug susceptibility states, and underscore the potential for additional biological differences resulting from neighboring loci.
Differential expression of genes with a role in fundamental biological processes such as RNA translation, ubiquitin-dependent catabolic processes and protein folding/trafficking was also observed in the pfcrt data set, albeit to a lesser extent. Gene sets associated with these pathways showed a greater enrichment in both 7G8-derived transgenic strains that were characterized by a decreased pfcrt transcript level, in comparison to the parental 7G8 line. This suggests an association between the degree of pfcrt expression and the regulation of cellular protein levels, regardless of PfCRT haplotype.
Intriguingly, levels of pfcrt transcripts, irrespective of the transporter polymorphism, also impacted sets of genes implicated in maintaining mitochondrial integrity and function, as indicated by the significant decrease in expression of genes coding for multiple subunits of the translocase complexes of the mitochondria inner membrane . In contrast, the transgenic strains that differ in the nature of the residue at position 76 in PfCRT displayed a differential enrichment of gene sets associated with amino-acyl tRNA synthases and ligases. Several of the genes coding for these elements, which play a central role in RNA translation, were more highly expressed in a stage-specific manner in the transgenic control in comparison to the back-mutant strain. These findings suggest that the impairment of PfCRT transport function through the acquisition of resistance mutations might impact the cellular level of amino acids. This hypothesis is consistent with the observation that PfCRT polymorphisms affect hemoglobin catabolism and that CQ-resistant parasites such as 7G8 are characterized by a phenotype of excessive peptide accumulation, which might in turn impact the reserve of amino acids available for protein synthesis . Additionally, the differential expression of genes encoding putative transporters between the two transgenic strains suggest that PfCRT resistance mutations might more generally affect intracellular transport dynamics, possibly as a secondary effect of the peptide accumulation phenotype.
Interestingly, both pfmdr1 and pfcrt transcriptome data sets displayed differences in the level of expression of genes coding for invasion proteins. Higher transcript levels of rhoptry and merozoite surface proteins, or components of the glideosome were detected in parasite strains with either reduced pfmdr1 expression such as FCB pfmdr1_3′KD and 106/1 or with a PfCRT wild-type haplotype such as the back-mutant strain 7G8 pfcrt_T76K . This, added to the observation that both CQ-sensitive strains 106/1 and 7G8 pfcrt_T76K had a shorter IDC than the other parasites, suggests an impact of these genetic changes on growth rates and parasite fitness . Interestingly, while 106/1 and FCB have genome sequences that are almost identical  (suggesting a common lineage), our data indicate notable differences in both their PfCRT haplotype and their levels of expression from the pfmdr1 amplicon region. By comparing FCB, FCB pfmdr1_3′KD and 106/1, we could disentangle the transcriptional changes due to lower expression/gene copy variation in the chromosome 5 amplicon from the effect of different pfcrt genotypes. For instance, 106/1 appears to express higher levels of ribosomal protein genes compared to both FCB strains, suggesting a response to its particular PfCRT haplotype that lacks the CQ resistance-conferring K76T mutation. On the other hand, our data suggest that the transcriptional downregulation of the pfmdr1-containing amplicon on chromosome 5 (in 106/1 and FCB pfmdr1_3′KD ) might affect parasite physiology, notably through the reduced expression of genes coding for multiple transporters and components of the parasite digestive vacuole, such as ABC and drug/metabolite transporters and V-type ATPases. Such an observation was particularly striking when performing a comparative analysis of the transcriptomes by stage, demonstrating how such an approach can complement analyses of gene expression averaged across time points.
Our investigations across multiple lines uncovered evidence of co-regulation of pfcrt and pfmdr1, which both also positively correlated across the genome with a suite of genes coding for transporters, components of the parasite digestive vacuole that include peptidases involved in hemoglobin catabolism, or redox enzymes. Conversely, pfcrt and pfmdr1 transcription anti-correlated with a set of genes encoding proteins involved in RNA processing, including splicing factors. Broader biological processes including transcriptional regulation, translation and post-translational processing contained genes that showed either positive or negative correlations with pfcrt and pfmdr1. Overall this suggests the existence of an elaborate equilibrium in P. falciparum gene expression that maintains optimal states of parasite physiology that extend beyond the digestive vacuole.
Our study builds on the recent work from Siwo and colleagues who constructed pfcrt co-expression networks using transcriptional measurements at the early trophozoite stage for the progeny of a genetic cross between CQ-sensitive and CQ-resistant parental strains . Their analysis provided evidence that functional partners of both CQ-resistant and CQ-sensitive pfcrt include genes involved in hemoglobin catabolism, which agrees with our own observations. Additionally, the authors attributed divergence in co-expression networks between CQ-resistant and CQ-sensitive pfcrt to genes involved in DNA repair and histone acetylation. Interestingly, we found that expression of the histone acetyltransferase GCN5 (PF3D7_0823300) clustered with that of pfcrt and pfmdr1, further highlighting an association between the expression of these two transporters and general transcriptional processes.
Furthermore, our data extend prior observations of joint contributions of pfcrt and pfmdr1 polymorphisms to drug resistance phenotypes [22, 28, 66], including evidence of linkage disequilibrium, by now providing evidence of a strong correlation between pfcrt and pfmdr1 transcript levels. This has direct implications for drug resistance studies that measure pfmdr1 copy number as a marker of decreased parasite susceptibility to antimalarial drugs  by implying an previously unrecognized role for accompanying changes in pfcrt.
PfCRT and PfMDR1 are two key determinants of P. falciparum parasite susceptibility to antimalarial compounds. Both are transporters localized in the intra-erythrocytic digestive vacuole, whose primary functions in addition to carrying antimalarial drugs remain to date unknown. Our transcriptome profiling of various P. falciparum strains, whose differences in pfcrt or pdmr1 coding sequence or copy number contribute directly to multidrug resistance, provide insight into how the acquisition of these genetic changes impact parasite physiology beyond the digestive vacuole. These changes include peptide catabolism, digestive vacuole homeostasis, RNA metabolism and protein synthesis. Our findings, based on the development of an analytical method that we term Hypergeometric Analysis of Time Series, also reveal a closely coordinated co-regulation of pfcrt and pfmdr1 transcript levels. Of particular interest now will be to further study how these transcriptional crosstalks affect downstream processes, in particular at the level of the parasite proteome and metabolome.
All parasite lines described here were propagated under standard conditions  in RPMI-1640 medium with L-glutamine, supplemented with 50 mg/l hypoxanthine, 25 mM HEPES, 0.25 % sodium bicarbonate, 0.01 mg/ml gentamicin and 0.5 % Albumax II (Invitrogen, Carlsbad, CA, USA). Parasites were synchronized by two consecutive sorbitol treatments timed 12 h apart for three or more successive generations and allowed to recover for another parasite asexual blood stage cycle before initiating time point samplings. For each time course, cultures were progressively expanded to a volume of 250–300 mL at 3 % hematocrit with 8 to 10 % of tightly synchronous populations of late P. falciparum schizonts. Parasites were inoculated in a bioreactor (Applikon, Foster City, CA, USA) in the presence of fresh erythrocytes and allowed to reinvade for the next 2–3 h at high hematocrit (8 %). Gas and temperature conditions were monitored with a Bio Controller unit ADI 1030. The first time point of the series was determined by the peak of invasion, after which cultures were diluted to a final volume of 1,000 mL and 2 % hematocrit. At least 90 % of the parasites were in the early ring stage and in general, cultures reached a parasitemia of 10 to 20 %. Equal parasite samples were harvested every 6 h throughout the 48-hour IDC and immediately centrifuged. Pellets of parasitized erythrocytes were directly stored in Trizol® for subsequent RNA extraction and cDNA synthesis.
Total RNA was isolated and synthesis of first strand amino-allyl cDNA was performed as previously reported [69, 70] using 125U of Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA). Each reaction was concentrated on a Zymo DNA clean and concentrator-5 column (Zymo Research, Irvine, CA, USA) and labeled with Cy5 dye (GE Healthcare, Piscataway, NJ, USA). The reference RNA pool consisted of Cy3-coupled cDNA samples prepared from RNAs representing all developmental stages at 6-hour intervals of the IDC of the 3D7 line. Equal amounts of labeled samples from each time point and reference pool were subjected to array hybridization for 16–18 h at 65 °C on 70-mer P. falciparum microarray chips that contained 10,416 features and represented all 5,363 coding genes as previously reported . Data were acquired using a GenePix 4000B scanner and analyzed with the GenePix Pro 4 software (Molecular Devices, Sunnyvale, CA, USA).
Automatic definition of hybridization spots was refined by manual inspection, and acquired raw data were initially filtered to exclude poor quality spots. The Cy5 and Cy3 intensity channels were separately normalized using the mean difference between the median foreground intensity and the median background intensity of a set of representative probes. This set included probes that contained more than 50 % of the pixels with intensities higher than 2 standard deviations above the background pixel intensity for both Cy5 and Cy3 signals, displayed at least 200 expression units and a regression correlation coefficient greater than or equal to 0.75 . After background subtraction, the log2 of the (Cy5/Cy3) intensity ratio was extracted for each probe and gene expression ratios were computed using the median of the probe level values. For genes with values for at least 6 of 8 time points, missing values were imputed using linear model prediction based on PCC values and accounting for mean and standard deviation differences.
Transcriptomes of all lines were reconstituted using the FFT method (fft() function in R) to sort genes according to phase, and impute expression levels at 376 unassayed time points, one for each 7.5 min of the 48-hour IDC. To correct for variations in developmental stage speed between parasite lines, dynamic time warping  was applied to determine the optimal post-invasion time point (corresponding to the first sampling time point) along the IDC using the PCC between each pair of imputed points. Each parasite line was aligned using the 3D7 transcriptome as reference . The alignments were confirmed by applying PCA. The first two principal components, derived from approximately 3,000 dimensions of expression data, accurately recapitulated the progression of the parasite lines throughout the IDC, while succeeding components focused on differences between parasite lines.
To assess gene expression level differences between parasite lines, the area under the curve (AUC) of absolute gene expression plots was computed on the full 384 time point data and the fold change was calculated as the difference between AUCs in pairwise comparisons. OPLS-DA (Orthogonal Partial Least Square-Discriminant Analysis) was used to create disjoint PCA models for each parasite line  and exploit class-orthogonal variation, thereby permitting the identification of genes expressed out of phase. Both approaches resulted in a similar gene ranking with a Spearman correlation of 0.89. To determine significant variation between parasite lines, a cut-off of >1.5-fold difference in gene expression was assigned.
All values were normalized using a background pool constituted of 11 transcriptome data sets that constituted a baseline for gene expression fold change and gene set enrichment. In addition to that reported in the present study, the pool included two IDC transcriptome data sets generated for Dd2 parasites pressured long-term with pulses of dihydroartemisinin (the 3b1 line) and the non-pressured parent , as well as the transcriptome data set previously reported for the 3D7, Dd2 and HB3 strains [46, 48]. This background pool was used to generate 55 pairwise comparisons (or 110, if taking into account analysis in both directions) and create a distribution of fold changes for each analysed gene, which was then used as a normalizing factor. When comparing two lines, only the remaining nine were used to build the fold change distribution. The analysis was conducted using a threshold of two or three standard deviations above this mean fold change. This method, based on a large dataset of pairwise comparisons, enables us to identify even genes for which expression changes are of small amplitudes.
To analyze gene sets, we developed an algorithm that we termed Hypergeometric Analysis of Time Series (HATS). The source code and pseudocode describing HATS are provided in Additional file 25. HATS is based on GSEA  and consists in assigning a rank probability to each gene in a given gene set using GSEA and then applying hypergeometric distribution instead of a weighted Kolmogorov-Smirnov-like statistic  to evaluate the enrichment score. To this end, 1,000 random permutations of the data were generated, and on each of these permutations we calculated gene rank probabilities P for each gene set. These probability values were averaged per gene set across the 1,000 permutations and used as a scaling factor defined as -log10(P) to generate a corrected enrichment score from that originally computed by GSEA. The false discovery rate (FDR) was estimated by comparing the distributions of the actual enrichment scores to those derived from the random permutations. Noise reduction was achieved by forcing all FDR values to monotonically decrease at the tails. Finally, theses results were then normalized by comparing them to the pool of 11 transcriptome datasets, across which pairwise GSEA comparisons were conducted for all possible combinations. Means and standard deviations of the pool-derived enrichment scores for each gene set were computed and used to generate normalized enrichment scores and remove sets that were stochastically expressed. We applied a cut-off for normalized gene set ranks of 0.1 for negatively enriched gene sets and of 0.9 for positively enriched gene sets.
Results were also analyzed by parasite developmental stages, using time windows corresponding to ring, trophozoite, and schizont stages . Stage-associated enrichment was calculated by averaging gene expression across each developmental stage using the AUC derived from the imputed 384-time point curve and then performing GSEA. As before the enrichment scores were calculated using the hypergeometric distribution and the values were normalized using the background pool of transcriptome datasets.
Gene sets were assembled from gene ontology (GO) annotations , the Malaria Parasite Metabolic Pathway [76, 77], KEGG  and InterPro  databases, and literature searches. A summary of these gene sets is provided in Additional file 24: Table S16. We extracted the GO categories for P. falciparum from PlasmoDB (www.plasmoDB.org) such that 1,664 GO categories involving 4,415 genes were included. On average, there were 3.85 categories per gene, while each GO category contained an average of 10.2 genes. There were 16,997 GO category labels applied in total, covering 69.3 % of the genome. On the other hand, 3,888 genes were labeled with an InterPro signature, averaging 3.09 labels per gene. There were 11,997 InterPro labels applied in total, covering 61.0 % of the genome. Finally, the metabolic pathways of Plasmodium were extracted from the KEGG database, such that in total 182 metabolic pathways were detailed, averaging 36.7 genes, with pathways ranging from two genes to 342 genes. 2,938 genes were part of at least one pathway, averaging 2.3 pathways per gene. There were 6,671 metabolic pathway assignments applied in total, covering 46.1 % of the genome.
Overall, 7,682 total labels were applied, assigning 5,184 genes to 6,427 gene sets. This accounts for 81.4 % of the genome, each gene being a member on average of 9.2 sets, with the most labeled gene being represented in 45 sets. The portion of the genome that was not represented in these sets included genes having no ascribed functional annotation. Over- or under-representation of a group of functional categories in comparison to all other categories was determined using hypergeometric distribution. Any comparison with a P-value < 0.01 was retained.
Hierarchical clustering was performed using PCC values obtained from all pairwise comparisons across all 11 transcriptome data sets using ratio of log2-transformed and normalized gene expression values.
Real-time PCR was performed in a DNA Engine Opticon 2 detector (Bio-Rad, Hercules, CA, USA) to assess transcript levels and gene copy numbers using the iQ SYBR Green Supermix (Bio-Rad). Parallel amplification reactions were carried out in 96-well plates in 25 μL volume containing 0.3 to 0.5 μM of each forward and reverse primer, with all samples run in triplicates. Measurements were performed on three independent occasions. Primer sequences and real-time PCR conditions are listed in Additional file 27: Table S18.
For quantitative RT-PCR, RNA was treated with DNase I (Ambion) to remove possible traces of contaminating genomic DNA. cDNA was prepared by reverse transcription from 5 μg of total RNA using a mix of oligodT (Invitrogen) and random hexamers (Invitrogen). To assess transcript levels, cDNA templates were diluted 60- to 80-fold. Relative quantification of transcripts was performed using the standard curve method, using 10-fold dilution series of genomic DNA harvested from the parental strains 7G8 or FCB. cyp87 (PF3D7_0510200, previously PFE0505w, Forward 5′ AAACGGGAGATCCTTCAGGT, Reverse 5′ AAGGACATGGGACAGTGGTT) was used as the reference control gene.
To assess gene copy numbers within pfmdr1 amplicon, 50 ng of template genomic DNA was used to measure each gene copy number relative to the single-copy cyp87 or β-tubulin (PF3D7_1008700, previously PF10_0084) genes. Assays were normalized using standard curves that were generated by 10-fold dilution series of genomic DNA from 3D7, known to have a single copy of pfmdr1 amplicon. Copy number results were rounded to the nearest integer.
The data sets supporting the results of this article are available in the GEO (Gene Expression Omnibus) functional genomics data repository at the NCBI, under the accession number GSE75807 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE75807).
Funding for this work was provided in part by the NIH (R01 AI50234 and AI109023 to DAF, the NIH Director's New Innovators award 1DP2OD001315 to ML, and support for ML from the Center for Quantitative Biology P50 GM071508), as well as the Burroughs Wellcome Fund (New Investigators in Pathogenesis of Infectious Diseases) to DAF and ML. We thanks Michael Muhle for valuable discussions and help with the Orthogonal Partial Least Square-Discriminant Analysis.
SHA, ML and DAF designed the experiments. SHA performed the experiments. EK assisted with the DNA microarray hybridizations. SHA, DS, ML and DAF analyzed the data. SHA, DS, and DAF wrote the manuscript. All authors read and approved the final manuscript.
World Malaria Report 2015 - full report. http://www.who.int/malaria/publications/world-malaria-report-2015/en/ 2014:1–242.


text:
Cinewax is looking for volunteer writers and content creators
par CINEWAX MEDIA 12 mai 2020
Cinewax is looking for volunteers for its new collaborative media dedicated to African cinema and new talent!
Launched in 2015, the Cinewax association aims to increase the visibility of films and artists from the continent and diasporas. In addition to the exhibition of films in partnership with cinemas, last year we launched the Online African Film Festival (OAFF), the first online African film festival.
Articles, interviews, surveys, videos...We want to make African cinema even more attractive and exciting by using a variety of formats!
We are a team of passionate volunteers, most of whom are in the Paris region. But we work remotely.
For the writing, we are looking for volunteer writers and community managers.
For the video part, we are looking for volunteer editors, sound engineers, photographers, and cameramen who can accompany us.
WHY A MEDIA PLATFORM?
Images have a real impact on our vision of the world and our relationship with others.
Our audience needs to be represented on the screen and to see authentic stories.
Movies are more than just stories. They say who we are, what we want, and how we see our world. They help us to have a critical look at our society, and to build our identity.
We believe it is urgent to show the world the richness of African creativity and to allow everyone to have access to positive and critical images from the African continent.
We accept difference. We all carry within us a hybrid culture, a history, and identities as varied as they are enriching, paths that deserve to be told.
We defend "made in Africa" as a symbol of authenticity and a unique point of view on our reality.
We carry the voices of thousands of African and afro artists and creatives, from around the world, who want to share their vision. These voices are not heard today.
We are exploring a new generation of talent and the newest trends in the African film industry, along with others.
We are looking for motivated and free-spirited writers, who can defend their ideas and have a perspective on the world.
If you see yourself in our values, if you are passionate about culture, creativity, and you are eager to discover African cinemas, if you can write, if you want to embark on the adventure of the birth of a young media platform, contact us by following the instructions below!
We can't wait to meet you!
PLEASE READ BEFORE CONTACTING US
1. Please read the media presentation for more information.
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3. Please send us your CV along with a brief presentation of yourself and why you want to join us.
- For writers, present a topic that interests you and how you want to deal with it, or a film review, or interview you already completed.
- For the production (photographers, editors, cameraman/woman) you can offer us content, or share your achievements.
Contact us at media@cinewax.org
OUR CURRENT OPPORTUNITIES
(as of May 9th 2020)
Volunteer Editor in Chief
To manage the production of articles and the team of writers. Experience as a journalist or in media is required. You will need to manage and control the schedule, and manage a team.
6 hours a week, give or take.
Volunteer writers
To write the articles and carry out our interviews, through the themes of our editorial. You can work in teams or solo, there are weekly briefings, you will search for topics and meet directors and actors;)
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Retour à African stories

redpajama_set_name: RedPajamaCommonCrawl


INDIANAPOLIS — The NFL draft will feature one of the most talented and deepest group of defensive linemen in league history.
standout who played at Ohio State, has a chance to be considered the best player of them all.
"It would be a dream come true," Bosa said Saturday at the NFL scouting combine when asked about potentially being the No. 1 overall pick.
Bosa will participate in a number of football drills during the combine on Sunday, along with several defensive ends and defensive tackles who could potentially join him as first-round picks.
Alabama defensive tackle Quinnen Williams, who could also be a top-five pick, considers himself fortunate to be in the loaded defensive line group.
6 foot 2 — had a horse named Oreo he credits with helping him become fearless.
ried about no 300 pounds," Oliver said regarding opposing offensive linemen.
Florida State defensive end Brian Burns, a former American Heritage Plantation High standout, could have benefited from a senior season with the Seminoles. But he has a unique, rangy skill set that intrigues NFL scouts and executives at the defensive end and outside linebacker positions.
Florida defensive end Jachai Polite, who is roommates at the combine with Bosa, is trying to find a balance between staying humble and promoting himself.
Clemson's trio of Clelin Ferrell, Christian Wilkins and Dexter Lawrence — who were once coach by new Miami Dolphins defensive line coach Marion Hobby at the school — are also in the mix.
Mississippi State teammates Montez Sweat and Jeffery Simmons, Boston College defensive end Zach Allen or Ohio State defensive tackle Dre'Mont Jones could be first-round selections as well.
Michigan defensive end Rashan Gary may have been the most assertive when discussing where he stands among the competition.
"I'm the best player in this draft, offensively or defensively — period," Gary said.
unique college careers out of all highly-touted prospects in the draft. He thought he was going to have a standout junior season until he suffered a bilateral core muscle injury during the third game of the year at TCU on Sept. 15.
The injury occurred in the groin/lower abdomen area, where Bosa said he had both sides surgically repaired because there was risk for future injury if only one side was fixed.
Bosa left Ohio State one month later, to preserve his health and first-round potential, instead of trying to recover from the injury and play again in college.
"When it happened and I got home from that TCU game, I was lying in bed. I could barely get up.
It was one of the darkest moments of my life so far," Bosa said.
Bosa does not want to think about how Ohio State's season, which was coach Urban Meyer's last with the Buckeyes, would have unfolded if he was in the mix.
But he is grateful for his family and support system for helping him get through the most difficult juncture in his life.
in Orange County, Calif., until the NFL season ended before moving back to Fort Lauderdale for the offseason.
Now, he has a chance to make some family and NFL draft history.
John Bosa, Nick's father, and Eric Kumerow, Nick's uncle, were both selected by the Miami Dolphins in the 1987 and 1988 NFL drafts, respectively. They played three seasons each in Miami.
Nick Bosa has a chance to be the fourth first-round pick in his family, with a chance to be drafted the highest.
Ohio State defensive lineman Nick Bosa, a star at St. Thomas Aquinas High, believes he can be the top pick in this year's NFL draft.
Ohio State's Nick Bosa arguably is the best of a deep class of defensive linemen eligible for the draft.


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For faster navigation, this Iframe is preloading the Wikiwand page for 1954 in British television.
1954 in British television
Overview of the events of 1954 in British television
List of years in British television (table)
Science +...
This is a list of British television related events from 1954.
11 January – The first weather forecast with an in-vision presenter is televised in the United Kingdom. The first weather presenter was George Cowling.[1]
9 April – The Grove Family, generally considered the first British TV soap opera, debuts on the BBC Television Service.[2][3]
5 July – First actual news bulletin, News and Newsreel, aired on the BBC Television Service, replacing Television Newsreel.[4]
30 July – The Television Act 1954 is given Royal Assent. It authorises the setting up of the infrastructure for British commercial television.
7 October – BBC Television covers a party political conference for the first time when it broadcasts from the Conservative Party Conference in Blackpool.
12 December – The BBC Television Service screens its famous, and controversial, adaptation of George Orwell's Nineteen Eighty-Four.
30 December – The first BBC Sports Personality of the Year ceremony is presented from London's Savoy Hotel. It has aired annually ever since.
The British Academy Television Awards, the most prestigious awards in the British television industry, are first awarded.
BBC Television Service
4 January – The Cabin in the Clearing (1954)
16 January – The Lost Planet (1954)
19 January – Show Case (1954–1955)
27 January – Friends and Neighbours (1954)
6 February – Clementina (1954)
30 March – The Wide, Wide World (1954)
31 March – Gravelhanger (1954)
9 April – The Grove Family (1954–1957)
21 April – Running Wild (1954)
11 May – The Windmill Family (1954)
12 May – The Dancing Bear (1954)
12 May – Fast and Loose (1954–1955)
7 June – Emney Enterprises (1954–1957)
15 June – The Gentle Falcon (1954)
10 July – Happy Holidays (1954)
13 July – Dear Dotty (1954)
14 July – The Six Proud Walkers (1954)
3 August – Paradise Island (1954)
16 August – Stage by Stage (1954–1955)
25 August – Crime on Our Hands (1954)
1 October – And So to Bentley (1954)
13 November – Fabian of the Yard (1954–1956)
21 December – Zoo Quest (1954–1963)
24 December – Carols From Kings (1953-present)
Independent productions
The Halls of Ivy (produced by ITC Entertainment)
The Vise (produced by Danziger Productions)
Continuing television shows
BBC Wimbledon (1927–1939, 1946–2019, 2021–2024)
The Boat Race (1938–1939, 1946–2019)
BBC Cricket (1939, 1946–1999, 2020–2024)
Muffin the Mule (1946–1955, 2005–2006)
Come Dancing (1949–1998)
Andy Pandy (1950–1970, 2002–2005)
Flower Pot Men (1952–1958, 2001–2002)
Watch with Mother (1952–1973)
The Appleyards (1952–1957)
All Your Own (1952–1961)
Rag, Tag and Bobtail (1953–1965)
The Good Old Days (1953–1983)
Panorama (1953–present)
Ending this year
Television Newsreel (1948–1954)
6 January – John Sparkes, comedian
20 February – Anthony Head, actor
16 March – Jimmy Nail, actor and singer
17 March – Lesley-Anne Down, actress
9 May – Nicholas Crane, geographer and TV presenter
21 June – Anne Kirkbride, actress (Deirdre Barlow in Coronation Street) (died 2015)
16 July – Nicholas Frankau, actor
26 August – Steve Wright, broadcaster
27 August – Andrew Marshall, scriptwriter
8 September – Anne Diamond, journalist and television presenter
Helen Lederer, comedian and actress
Martin Sixsmith, journalist, author and radio and television presenter
3 October – Jeff Randall, journalist and television presenter
14 October – Carole Malone, journalist and presenter[5]
19 October – Ken Stott, actor
24 November – Susan Gilmore, actress (Avril Rolfe in Howards' Way)
1 December – Alan Dedicoat, newsreader and the National Lottery's "Voice of the Balls"
5 December – Hanif Kureishi, novelist and screenwriter
22 December – Hugh Quarshie, actor
1954 in British music
1954 in the United Kingdom
List of British films of 1954
^ "First BBC television weatherman George Cowling dies". BBC News. 26 December 2009. Retrieved 27 December 2009.
^ "The Sunday Post: Soap on the Box". BBC Genome Blog. 3 July 2016. Retrieved 27 January 2019.
^ "The Grove Family: A House of Your Own". 2 April 1954. p. 44. Retrieved 27 January 2019 – via BBC Genome.
^ "BBC launches daily TV news". BBC On This Day. 1954-07-05. Retrieved 19 May 2009.
^ "Carole Malone". British Film Institute. Retrieved 21 January 2017.
Years in television: 1954


At the beginning of October, Amazon released its newest advertising format – Amazon Media Group, which is basically Amazon's own display advertising network. With over 150 million visitors to Amazon.com each month, who are all at least minimally interested in purchasing something, prepare to meet a new advertising powerhouse.
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Granted, like any major display campaign, Amazon's marketplace doesn't come cheap. But if you're a big brand, with significant media dollars to spend, I would seriously consider testing Amazon's marketplace. Shoppers go to Amazon to buy. They are in that mindset, and with perks like Amazon Prime, impulse shopping is likely at an all-time high (at least it is in my house). Amazon has mastered the online shopping experience, and continues to be the gold-standard in shopping cart best practices. Combining highly-targeted display advertising with a site where people can easily purchase your advertised products sounds like a no-brainer to me.
Learn more about Amazon's Media Group.