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Home / Tax News / February 2020 / China
February 2020 / China
STA Clarifies Export Tax Refund Policies During Epidemic Period
The State Taxation Administration recently released the Circular about Export Tax Refund (Exemption) During the Prevention and Control of Coronavirus. During the epidemic period, taxpayers can handle tax affairs like making registrations for tax refund & exemption, issuing tax certificates and making tax refund (exemption) declarations through "non-contact" means. After receiving applications, tax agencies will only examine electronic documents and grant tax refund (exemption) status if there is no suspicion of tax fraud. From February 3, 2020 until the end of the epidemic period, local tax agencies shall not be considered overdue if it postpones tax refund or sends a reply later than expected due to the epidemic; tax agencies can send online notices to taxpayers about whether their tax refund (exemption) applications have been approved.
China Offers Provisional Respite to Companies in Social Security Contributions
The Ministry of Human Resources and Social Security, the Ministry of Finance and the State Taxation Administration recently issued the Circular about Provisional Reduction and Exemption of Corporate Contributions in Social Security. According to the circular, in provinces other than Hubei, micro, small and medium-sized enterprises will be eligible for a waiver of pension fund, unemployment and workplace safety insurance contributions from February to June 2020. Large companies will see their contributions halved for three months. Companies of all types in Hubei who have participated in these insurance schemes will be exempted from the above contributions from February to June. These policies are estimated to reduce enterprises' cost by about 500 billion yuan. The circular also said that the central government's reallocation ratio of basic endowment insurance funds will increase to 4% in 2020 to offer stronger support to hard-hit regions.
Enterprises Can Negotiate with Employees to Suspend Contributions to Housing Provident Fund
In order to support companies to resume production and operation and facilitate tax declarations by corporate taxpayers, the State Taxation Administration recently issued a circular to extend the deadline for tax declaration in February 2020 to the end of the month. According to the circular, for taxpayers that make tax declarations on a monthly basis, except those based in Hubei province, the deadline for tax declaration will be further extended to February 28, 2020 (Friday). If taxpayers are still unable to declare tax before that date, they can give legitimate reasons to the tax bureau and make an application to defer tax declarations. The tax bureau will not impose surcharges for overdue tax payment, not impose administrative penalties and not adjust its tax credit rating.
STA Says Hard-hit Enterprises Must Submit Statement for Extending Period of Loss Carryover
The State Taxation Administration recently issued the Announcement about Tax Collections during the Coronavirus Epidemic. According to the announcement, if an enterprise hit-hard by the epidemic wants to extend the period for loss carryover, it should submit an electronic version of the Statement of Eligibility for Extending the Period of Losses Carryover when it pays corporate income tax for 2020; if a taxpayer is eligible for privileges on value-added tax and consumption tax, it can make tax-free declarations and keep relevant tax documents for future examinations.
Ministry of Commerce PRC Urges More Efforts to Serve Foreign Enterprises and Attract Investments
The Ministry of Commerce issued on February 10, 2020 the Circular about Strengthening Services to Foreign Enterprises and Attracting Investments During the Coronavirus Epidemic. The circular pointed out that local commerce departments should take swift actions to help foreign companies resume normal production and business operations, use Internet platforms for staff recruitment and meet their labor needs. Officials should closely monitor the progress of large foreign investment projects, increase communications with foreign investors and strive to sign project contracts as early as possible.
The State Council decided to implement 20 reform measures nationwide or in eight comprehensive innovation reform pilot zones
The eight areas refer to the Beijing-Tianjin-Hebei region, Shanghai municipality, Guangdong province (Pearl River Delta), Anhui province (Hefei, Wuhu and Bengbu cities), Sichuan province (Chengdu, Deyang and Mianyang cities), Wuhan of Hubei province, Xi'an of Shaanxi province, and Shenyang of Liaoning province, where 36 reform measures were carried out in 2017 and 2018. Seven reforms will help further innovation of technological finance. For example, banks and investment institutions will be encouraged to support innovation-driven technology companies by establishing a long-term market-based cooperation mechanism, and a financing evaluation system will be built for small and medium-sized technology companies. And to promote innovation of technology management, six measures will be adopted, including one to pool strengths to create a shareholding alliance among cutting-edge technology industrial chains, and another to promote the mixed-ownership reform in related State-owned enterprises in old industrial bases. In addition, to protect intellectual property rights, two measures will be implemented to establish platforms providing inter-regional lawsuits and notary services. And finally, one measure will be applied to forge a new vocational education development mechanism and four to further civil-military integration. The circular also required efforts to promote the newly issued 20 reform measures while consolidating those released in 2017 and 2018. The systematic integration of the measures in the same areas and the collaboration among the measures in different areas will both be strengthened. Local authorities should be clear about their responsibilities and take actions in line with specific conditions, and related departments of the State Council should strengthen support and guidance. The National Development and Reform Commission and the Ministry of Science and Technology should take the lead in the reforms, especially in overall planning and coordination, strengthening policy interpretation, summarizing and publicizing typical experiences, it said.
State Council Adjusts Regulations in Pilot Free Trade Zones
The State Council recently issued the Circular of Temporarily Adjusting Some Administrative Regulations in Pilot Free Trade Zones, in order to ensure measures of reform and opening-up are implemented well in accordance with the law. According to the announcement, three administrative rules will be adjusted in the pilot free trade zones. Under the Regulation on the Administration of Commercial Performances, foreign investors and Taiwan investors can establish wholly-owned performance brokerage agencies, joint ventures in art performing business can be established as long as the Chinese partners have controlling stakes. The Provisions on the Administration of Foreign-funded Telecommunications Enterprises have expanded coverage from Shanghai free trade zone (28.8 square kilometers) to all free trade zones in China. Under the Regulations on the Administration of Printing Industry, wholly foreign-owned printing companies can be established in China.


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Abstract: This review focuses on the last decade´s development of the computational stereopsis for recovering three-dimensional information. The main components of the stereo analysis are exposed: image acquisition and camera modeling, feature selection, feature matching and disparity interpretation. A brief survey is given of the well known feature selection approaches and the estimation parameters for this selection are mentioned. The difficulties in identifying correspondent locations in the two images are explained. Methods as to how effectively to constrain the search for correct solution of the correspondence problem are discussed, as are strategies for the whole matching process. Reasons for the occurrence of matching errors are considered. Some recently proposed approaches, employing new ideas in the modeling of stereo matching in terms of energy minimization, are described. Acknowledging the importance of computation time for real-time applications, special attention is paid to parallelism as a way to achieve the required level of performance. The development of trinocular stereo analysis as an alternative to the conventional binocular one, is described. Finally a classification based on the test images for verification of the stereo matching algorithms, is supplied.
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Associations arrange Field Days for Members to gain more experience in beekeeping – working on a Top-bar hive.
Members of the Knysna Beekeepers Association, a small group of local enthusiasts share their knowledge and experience with other caring beekeepers in this small community. What country beekeeping is all about!!


Canberra centre Joseph Leilua can escape an NRL suspension with an early guilty plea after being hit with a grade-one dangerous contact charge.
Leilua hit Newcastle's Tim Glasby with an elbow to the head in the 68th minute of his side's 17-10 win over the Knights on Friday.
The NRL judiciary on Saturday charged Leilua with a grade-one offence. Given his clean record, Leilua will avoid missing any football with an early guilty plea, but could miss one match if he contests the charge and loses.


Enugu Rangers and Akwa United are the leading teams in Group A and B of Nigeria Professional Football League respectively after the impressive results they recorded in their matchday 15 games on Sunday.
Elsewhere, Akwa United put up an impressive display as they thrashed Kano Pillars 3-0 at Nest of Champions in Uyo on Sunday.
The Promise Keepers who got a 1-1 draw in the reverse fixture tore their fierce rivals apart with goals from Ndifreke Effiong, Emmanuel Charles and Kodjovi Dadzie. The victory ensured that they got maximum points that would keep them top in Group B for another weekend.
(Uche Ihuarulam 18′ PEN, 52′).


The food we eat, both what we eat and how we eat it, may be the most significant witness to creation care Christians can perform. With every bite we communicate what we think about land and water, fellow animals, fellow humans, and God as the Provider of the many gifts of nurture we daily consume.
along with its attractive display and packaging, makes us think that food will always be in plentiful and secure supply, provided we have the money. The availability, appearance, and relatively inexpensive price of foods compel us to believe that today's food production is an unmitigated success. When it comes to food, it seems there is little to worry or ruminate about other than its (still) unequal distribution. Recent books by Michael Pollan (The Omnivore's Dilemma), Barbara Kingsolver (Animal, Vegetable, Miracle), Raj Patel (Stuffed and Starved), and Paul Roberts (The End of Food), and the film Food Inc. demonstrate that our easy confidence may be misplaced.1 It is true that today's industrial agriculture is producing more food calories than we have ever seen before. Moreover, global supply lines of meats, oils, coffee, grains, fruits, and other commodities—all heavily reliant on a steady flow of cheap fossil fuels— along with the consolidation of food processing and distribution into the hands of a few giant food corporations, makes it possible for many of these calories to be inexpensively priced. But these copious and cheap calories are coming at a very high price to our soils, waters, atmosphere, animals, and agricultural workers. Around the world small landholders are being forced off their land (to make way for commodity production) and then finding themselves and their families food insecure. Meanwhile, the processing and artificial enhancement of many of our foods is proving to be a serious detriment to our health and the health of creation as a whole. The upshot of today's global, industrial food economy means that many Christians now find themselves in a position where our eating is a desecration to God. It is not as though we daily and deliberately choose to violate the land and its creatures. Rather, the food most readily available in stores, restaurants, schools, and hospitals simply is the end product of processes that have put profitability, production efficiency, marketability, and convenience above creaturely care, animal contentment, eater health, and farmer and food worker justice. To eat in a way that honors God and cares for creatures takes time, understanding, and daily work. It requires that we make the protection and nurture of our lands and waters a top priority. It also presupposes wide and detailed knowledge about the sources, life, and death that support and permeate our food, knowledge that fewer and fewer of us have.
fine, even exotic, food whenever we want it. How many of us know that among today's seven billion people, over one billion suffer from over-nourishment—that is, from eating too many unhealthy calories—and that millions more suffer from various eating disorders (like anorexia and bulimia) meant to cover up deep personal suffering and pain? Jesus is alerting us to the real possibility that we can have an unhealthy relationship to food (and to other eaters when we set ourselves up to be the Food Police). He is warning us not to become When we eat so as to remember God, which gluttons or self-righteous. Instead, he wants us to is to eat properly, we eat with an become people who "strive first for the kingdom of appreciation for how food is a blessing and God and his righteousness" gift. This is no small thing, because it means (Matthew 6:33), simply taking it for granted that when we will also relate to every other creature in we do this the food creatures need to eat will be provided. a way that honors God. By telling us not to worry or obsess about food Jesus is not saying that food is unimportant in God's kingdom. If that were the case it would be difficult to understand why Jesus spends so much time in the Gospels feeding people, eating with outcasts and sinners (cf. Luke 15:2), and celebrating the goodness of food shared with others. Jesus takes no delight in the hunger or ill-health of others. We must not forget that Jesus was derided by religious leaders as "a glutton and a drunkard" (Luke 7:34). As one commentator on the Bible has said, in Luke's Gospel Jesus is either going to a meal, at a meal, or coming from meal! In his life and ministry food clearly mattered to Jesus. It should matter to us too. The question is how.
Y We are given a clue to what a proper relationship to food looks like when we turn to one of the foundational stories in Scripture. In Genesis 3 Adam and Eve commit the sin of eating from the tree of the knowledge of good and evil. Was the sin eating? Clearly not since God had plainly said that they could eat freely from the many trees that are "pleasant to the sight and good for food" (Genesis 2:9). They were, however, forbidden to eat from the tree of the knowledge of good and evil because in eating from it they would die (2:17). There are forms of eating that lead to life, even kingdom-of-God life, and there are forms of eating that lead to death. How should we characterize the difference? A little further in the story we learn that the fruit from the tree of the knowledge of good and evil was good for food and a delight for the eyes.
the world of food is refashioned to serve and please us, its ability to feed and nurture others is diminished, precipitating needless pain and death; human eating becomes a destructive race to control and exploit the land, its creatures, and its eaters. Lest we think this a mere theological abstraction, consider how this dynamic is being worked out in today's industrial meat production system. Because of our desire to have the cheapest food possible, millions of chickens are raised in a manner that violates their being and is an insult to God. Rather than being free to roam and range, chickens are crammed into perpetually dark facilities where they are fed a steady diet of food supplements (to make them grow faster) and antibiotics (to prevent them from dying in the disease-inducing facilities). Many of these chickens have been genetically re-engineered so they will grow bigger breasts (Americans crave white meat). Large breasts combined with rapidly accelerated growth means that many of these chickens find it difficult to walk. Some break their legs under the strain of their own weight. These are highly stressful and miserable conditions for the birds. Their stress and misery, however, are the direct result of our desire to eat chicken meat on terms that satisfy our desire for cheapness, volume, and convenience. In our eating we have become slaves to a food system that systematically degrades chicken life. The degradation that is being worked out in these chicken facilities is also being worked out in almost all aspects of today's industrial food system. Soils are being systematically eroded and then poisoned with heavy applications of ever more toxic herbicides. Water is being wasted and polluted by the same poisons and by the application of synthetic, fossil-fuel derived fertilizers. Cattle, sheep, and pigs are often being raised in similarly cruel conditions as chickens. Ocean fisheries are being harvested to exhaustion and beyond the ability of fish stock to replenish themselves. Farmers around the world are seeing cancer rates go up as they work with toxic agricultural chemicals (many of which are banned in the United States), or are struggling under mounting levels of debt incurred while purchasing expensive seed and fertilizer inputs. Viewed ecologically, today's industrial food production represents a system that is unparalleled in its destructiveness.3 Food production and consumption, rather than being the means of life and communion with God and creation, has become instead the means of ill health, exploitation, suffering, and death.
1 Michael Pollan, The Omnivore's Dilemma: A Natural History of Four Meals (New York: The Penguin Press, 2006); Barbara Kingsolver, Animal, Vegetable, Miracle: A Year of Food Life (New York: HarperCollins, 2007); Raj Patel, Stuffed and Starved: The Hidden Battle for the World Food System (Brooklyn, NY: Melville House Publishing, 2007); and Paul Roberts, The End of Food (New York: Houghton Mifflin Company, 2009). The documentary Food, Inc. (2009) directed by Robert Kenner is available on DVD from Magnolia Home Entertainment; a number of experts featured in the documentary contributed essays to the study guide Food, Inc. (New York, Participant Media, 2009) edited by Karl Weber. 2 Alexander Schmemann, Great Lent: Journey to Pascha (New York: St. Vladimir's Seminary Press, 1969), 94-95 (first italics my emphasis). 3 More detail on the destructiveness of today's industrial food economy can be found in the third chapter of my book Food and Faith: A Theology of Eating (New York: Cambridge University Press, 2011). 4 William Ralph Inge, "Confessio Fidei," in Outspoken Essays, Second Series (London: Longman's Green, 1926), 1-59, here citing 56.
is Research Professor of Theology, Ecology, and Rural Life at Duke Divinity School in Durham, North Carolina.


The southern double-collared sunbird is 12cm long. The adult male has a glossy, metallic green head, throat upper breast and back. It has a brilliant red band across the chest, separated from the green breast by a narrow metallic blue band. The rest of the underparts are whitish. When displaying, yellow feather tufts can be seen on the shoulders. As with other sunbirds the bill is long and decurved. The bill, legs and feet are black. The eye is dark brown. The male can be distinguished from the similar greater double-collared sunbird by its smaller size, narrower red chest band and shorter bill.
The call is a hard chee-chee, and the song is high pitched jumble of tinkling notes, rising and falling in pitch and tempo for 3-5 seconds or more.
More inforamtion about Southern Double-collared Sunbird Facts and Photos.


If for no other reason, it could be worth watching the PBS Ken Burns extravaganza on the US war on Viet Nam just to see how many hours and how much video imagery can be spent rehashing what establishment accounts have already been foisting on the public for decades.
No doubt about it, Burns, PBS and NPR are masters of what they do. They jump all over showering the viewer with imagery, factoids and interviews to make it seem like a wide range of incisive questions are being raised and all viewpoints are being aired. Rather than a basis for historical analysis, however, it's little more than ambiguity, equivocation, obfuscation and contradiction carefully kept within established parameters about US good intentions, innocent cultural misunderstanding, communist evil deeds, and sympathy for any US collaborators who were less murderous and corrupt than its first chosen partner Ngo Dinh Diem--in short, anything but the possibility that the US consciously instigated the war to maintain and expand its power in Asia.
Lying by omission is a standard political strategy, but PBS is not going to give naysayers an equivalent 18 hours to fill in what was left out. All we can hope for is getting a few words in edgewise. (For some specifics see http://vietnamfulldisclosure.org/index.php/burns-novick-documentary-brief-first-thoughts-episode-1/ ) Thus, my focus here will be on veterans.
One NPR commentator recently stated that many veterans may not watch the series because according to some "research," they will recall how unpleasantly they were treated when they got home. This idea of vicious or continuous attacks on returning veterans has been refuted many times. Veterans would be better advised to recall how their government treated them. More likely they do not want to watch and be reminded of how what they were told was at such variance with what they experienced.
Secondly, experiencing war violence as a defender of one's homeland is different from experiencing it as an invader. Of course, US GIs (and ARVN soldiers) were told that they were defenders--defenders of freedom against aggressive communists. Nevertheless, even if they were convinced, it would be very different from the experience of Vietnamese fighters who were literally defending their villages and family members from foreign invasion.
Veterans do need healing but there are few good options. They may feel better if they can be convinced that the terrible things that they did and saw were necessary, or even if unnecessary, were done in good faith. This is what they are likely to get from Ken Burns, but it is tantamount to embracing the rationalizations of their oppressors--the very ones who drafted them and lied to them--vs. confronting the violence and evil that they were forced to be part of as well as confronting those who planned and orchestrated it. The real terrorists live in Washington D.C. and wear 3-piece suits and wing-tip shoes, not black pajamas and sandals made from car tires.
For recent US wars, we now have the Support the Troops campaign, to avoid the past abuse supposedly suffered by returning vets at the hands of disrespectful war protestors. I suspect the real purpose is simply to suppress dissent. Toward the end of the VN war US soldiers like me were quite in agreement with the protestors if not ahead of them; hence the so-called "soldiers' revolt." In any case, today's Support the Troops movement forces vets and the public to suppress criticism of US wars by making criticism of war tantamount to criticism of vets or making support for vets tantamount to support for wars.
Lastly, apology could certainly hasten healing, not only to veterans and their families but also to the Vietnamese people as well, but it has not been forthcoming.
War makers have learned a lot from and since the VN war. They have learned that hiring mercenaries like the Kurds or turning cities into piles of concrete rubble from the air is preferable to American corpses coming back home in boxes to Pleasantville, America.
What the entire nation needs--civilians as much as vets--is a critical analysis of the history and cause of the war. Reporting vaguely that there were differences of opinion about the war among Vietnamese or Americans in the style of PBS pabulum is not the same as critically assessing the origins and motivations of those who initiated these policies and sent soldiers off to kill and die. The Kennedy who escalated the war by sending special forces troops to Vietnam to engage in covert warfare in May 1961 was the same Kennedy who authorized the Bay of Pigs invasion of the previous April. This tells us something, things that we won't get from politicians' speeches or press conferences"or most likely from PBS either.
These days presidents and their children do not go off to war. They are busy pursuing careers at Harvard or whatever. They will not ask the hard questions, not just about the VN war but about the patterns of US history from the Native American genocide to slavery to carpet bombing in Korea and Southeast Asia to drone killings in the Middle East. That will be up to the rest of us.
When Is Diversity Not Diversity?


This legal document is part of the collection entitled: Defense Base Closure and Realignment Commission and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 53 times . More information about this document can be viewed below.


Please consider sponsoring The 2019 Sedona Conference on Global Aspects of Patent Litigation to be held on November 19-20, 2019 at the historic Carton House estate in County Kildare, Ireland, just outside of Dublin. We will have capacity for 100 people (including faculty). For program page, please click here. For a working agenda, please click here.
We will be announcing the opening of registration in late February / early March. If you want your organization to be listed as a sponsor in this announcement, please let us know immediately.
Most importantly, your organization will be associated with this non-partisan, consensus effort to move the patent law forward in a reasoned and just way.
As markets and technologies grow increasingly interconnected around the world, patent litigants, both owners and defendants, are re-orienting their litigation strategies to reflect global realities. At the same time, changes in the legal landscape, in the U.S., Europe and Asia, are giving rise to both new risks of and new opportunities for patent litigation. Rapidly evolving case law, new guidelines from national competition authorities and other institutions, and legislative initiatives constantly shape and re-shape the ever more complex framework for both plaintiffs and defendants.
Our co-chairs and a distinguished faculty of judges, government officials, and counsel from around the world will lead the dialogue on topics identifying and explaining wide range of issues arising in international patent litigation, and comparative approaches across different international patent jurisdictions. This Conference will continue the in-depth dialogue from our 2017 Conference on Patent Litigation: Global Strategies for Managing Both Multifront Domestic and International Litigation of IP Assets and our 2018 Conference on International Patent Litigation: Aspirations Toward Attaining Effective Global IP Protection, and also from The Sedona Conference's extensive Working Group 9 and 10 (patent litigation) commentary drafting team efforts over the past five+ years which have been focused on the U.S. patent litigation space and are now expanding to a global perspective.
Please send me an email at cweinlein@sedonaconference.org if you would like to sign up as a sponsor, or if you have any additional questions.
Many thanks again for your support and involvement.
The Sedona Conference (TSC) is a nonpartisan, nonprofit research and educational institute dedicated to the advanced study of law and policy in the areas of antitrust law, complex litigation, and intellectual property rights.
The Sedona Conference Working Group Series was launched in 2002 and represents the evolution of The Sedona Conference from a forum for advanced dialogue to an open think-tank confronting some of the most challenging issues faced by our legal system today. The first Working Group (WG1) launched in 2002, and was dedicated to the development of guidelines for electronic document retention and production. Its first publication - The Sedona Principles Best Practices Recommendations and Principles Addressing Electronic Document Production - was cited in a report on Electronic Discovery by the United States Judicial Conference Advisory Committee on Civil Rules less than a month after publication of the first draft, and was cited in a seminal e-discovery decision in the Southern District of New York less than a month after that.
The Sedona Conference's Working Group 10 on Patent Litigation Best Practices (WG10) and Working Group 9 on Patent Damages and Remedies (WG9) have published numerous consensus, non-partisan documents over the past five+ years, collectively designed to move the law and practice of patent litigation forward in a reasoned and just way. Each publication reflects the collective wisdom and experience of members of all stakeholders in the patent litigation system, including the judiciary, the plaintiff and defense bars, patent prosecutors, and in-house counsel representing various industries. For the Executive Summary with links to all current publications, please click here.


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JLL is Top Real Estate Investment Advisory Firm in Asia Pacific for Sixth Year in a Row
Data from Real Capital Analytics reveals top dealmakers by volume in 2016
SINGAPORE, Mar 21, 2017 - (ACN Newswire) - JLL is the number one real estate investment advisory firm in Asia Pacific for the sixth consecutive year, based on data published by Real Capital Analytics (RCA), an independent body that analyses real estate transaction volumes worldwide.
According to the data, JLL tops the ranking in Asia Pacific, having achieved the highest value of investment deals overall in 2016 - a total of US$ 20.4 billion, which is 27.5 per cent market share in the region. The firm also scooped number one in the hotel sector with US$1.8 billion in hotel sales, a 41.8 per cent market share across the region.
Of the six real estate investment categories covered by the RCA ranking, JLL came top in three: retail and apartment, in addition to hotels.
"We are absolutely thrilled to be recognised for the sixth consecutive year in the RCA ranking," says Stuart Crow, Head of Asia Pacific Capital Markets, JLL. "This achievement is the result of great teamwork and a commitment to providing outstanding investment advisory services. We look forward to working closely with our clients to do even more great deals for them in 2017."
"Looking back to 2016, we saw a peak in real estate transaction volumes in Asia Pacific, with new investors attracted to this region, including sovereign wealth, and pension and insurance funds. These investors - whether they are in the region or outside - are allocating more capital to real estate so we expect to see more big deals and continued healthy volumes in 2017."
Scott Hetherington, CEO Asia, JLL Hotels & Hospitality, says: "We're delighted that once again we're the top ranked investment advisory firm in the hotels sector. This result really demonstrates the great talent we have in our team to be able to deliver value for our clients year after year. The hospitality sector continues its upward trend in Asia Pacific, so we are seeing a strong pipeline of deals for 2017 and beyond. In particular, we're hearing from investors that they're excited about opportunities in Japan, Greater China and the Indian Ocean."
For more information on RCA's methodology, visit http://www.rcanalytics.com.
About Jones Lang LaSalle, Inc.
JLL (NYSE: JLL) is a leading professional services firm that specializes in real estate and investment management. A Fortune 500 company, JLL helps real estate owners, occupiers and investors achieve their business ambitions. In 2016, JLL had revenue of $6.8 billion and fee revenue of $5.8 billion and, on behalf of clients, managed 4.4 billion square feet, or 409 million square meters, and completed sales acquisitions and finance transactions of approximately $136 billion. At year-end 2016, JLL had nearly 300 corporate offices, operations in over 80 countries and a global workforce of more than 77,000. As of December 31, 2016, LaSalle Investment Management has $60.1 billion of real estate under asset management. JLL is the brand name, and a registered trademark, of Jones Lang LaSalle Incorporated. For further information, visit www.jll.com.
JLL has over 50 years of experience in Asia Pacific, with 36,000 employees operating in 94 offices in 16 countries across the region. The firm won the 'World's Best' and 'Best in Asia Pacific' International Property Consultancy at the International Property Awards in 2016. www.ap.jll.com.
Vernia Lim
Email: Vernia.Lim@ap.jll.com
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redpajama_set_name: RedPajamaCommonCrawl


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A las veces, cazar pensamos do cazados quedamos.
A las veces, con tuerto hace el hombre derecho.
A las veces, cuesta más el salmorejo que el conejo.
A las veces, está la carne en el plato por falta de gato.
A las veces, la cabra bala por el cuchillo que la mata.
A las veces, la cabra por el cuchillo bala.
A las veces, lleva el hombre a su casa con que llore.
A las veces, más vale el vino que las heces; y de contino más vale el vino.
A las veces, miran más a las armas que a las barbas.
A las veces, ruin cadela roe buena correa.
A l'Ascension, la fille vaut le garçon, à la Pentecôte, elle en vaut 4 coûte à coûte.
A Lasciafare gli rubarono la moglie.
A Lasciafare glielo misero in culo.
A Lasciafare misero l'acqua in tasca.
A lasciar ci è sempre tempo.
A lass is a lad's leavings.
A lass that has many wooers oft fares the worst.
A lass that has many wooers often fares the worst.
A' lasses, but where do the ill wives come frae?
Wer müde ist, sucht Streit.
A lässt sich nich balde über a Tölpel warffen.
A late Easter, a long cold spring.
A late repentance is a dogs idea.
en] From/on the side; with confidence.
A' latrok szerzették a' jó törvényt.
A laughing ermine eats a goodly cobbler while the weasel dances to his own tune.
A laughing faced lad maks a lither servant.
A lausiger Schulze, a nisige Gemeind.
Wie die Läuse Nise zeugen, so pflegen sich die Mitglieder der Gemeinde nach dem Vorsteher derselben zu bilden und zu richten.
A lavar la testa all'asino ci rimetti il ranno e il sapone.
A lavar la testa all'asino ci si perde il ranno e il sapone.
A lavar la testa all'asino si perde il ranno e il sapone.
A lavare la testa all'asino ci si rimette acqua, spazzola e sapone.
A lavare la testa dell'asino si spreca il sapone.
A l'aventure, met-on les oeufz couver.
A laver la tête d'un âne, on perd son savon.
L'on n'y perd sa lessive.
A l'aveugle ne convient peinture, couleur, miroir, ni figure.
A l'aveugle ne duit miroir ni figure.
A l'aveugle ne duit peinture, couleur, miroir ne figure.
A lavrador descuidado os ratos comem o semeado.
A lavrador descuidado, os ratos lhe comem o semeado.
A lavrador preguiçoso levam os ratos o precioso.
A' law is no justice.
A lawsuit for a maravedi consumes a real's worth of paper.
A lawsuit is a fruit-tree planted in a lawyer's garden.
A lawyer and a cartwheel must be well greased.
A lawyer and a wagon wheel must be well greased.
A lawyer is sometimes required to search titles, and the client who thinks he has good right to an estate, puts the papers in his hands, and the attorney goes into the public records and finds everything right for three or four years back; but after a time he comes to a break in the title. So he finds that the man who supposed he owned it owns not an acre of the ground which belongs to someone else. I trace the title of this world from century to century until I find the whole right vested in God. Now to whom did he give it? To his own children. All are yours.
A lawyer looks at you with one eye, but he looks at your pocket with two.
A lawyer needs three bags: one full of papers, one full of money, and one full of patience.
A lawyer never go's to law himself.
Honesty shines with great advantage there.
A lawyer's fee and a harlot's wages are paid in advance.
A lawyer's gown 's lined with the selfishne's o' h's clients.
A lawyer's opinion is worth nothing unless paid for.
A layen hen is better than a stan'in mill.
A lazy boy and a warm bed are difficult to part.
A lazy chick that exposes itself will be a delicious meal for the eagle.
A lazy fly cannot have a taste of a fresh wound.
A lazy frost, a numbness of the mind.
A lazy hand is no argument of a contented heart.
A lazy horse is sure to stand near the chaff house.
A lazy man plants on a mad bark as an excuse for not planting.
A lazy man will be an astrologer.
A lazy man will remember he has not planted when is neighbour is harvesting.
A lazy man works twice.
A lazy man's farm is the breeding ground for snakes.
A lazy messenger is not short of excuses.
A lazy ox is little better for the goad.
A lazy ox is little the better for the goad.
A lazy person has to do a task twice.
Yiddish] A foiler tut in tsveyen.
A lazy person sweats while eating and shivers while working.
A lazy person with a nephew does not eat dry rice.
A lazy tailor finds his thread too long.
A lazy thief is better than a lazy servant.
A lazy woman is never virtuous.
A lazy youth, a lousy age.
A lazy, indolent church tends toward unbelief; an earnest, busy church, in hand-to-hand conflict with sin and misery, grows stronger in faith.
A l'è mej un cativ accordi ch'una bona sentenssa.
A leaden sword in an ivory scabbard.
A leader does not listen to rumors.
A leader does not rise for a squirrel.
A leader is he who tells his people what they must hear, not what they want to hear.
A leader is judged not by the length of his reign but by the decisions he makes.
A leader is not necessarily one who knows the way but one who thinks he knows the way.
A leader who does not take advice is not a leader.
A leader who understands proverbs reconciles difficulties.
A lean agreement is better than a fat judgement.
A lean agreement is better than a fat sentence.
A lean agreement is better than a good judgement.


text:
SMITH, Laura
SMITH, Laura Sue
Laura Sue Smith, age 79, passed away Tuesday, October 4, 2022. She was born March 20, 1943, in Hamilton, to the late Edward and Laura (nee Letsche) Frey. She is survived by her son Scott (Sally) Siegel; grandsons Max Siegel and Theo Siegel; brother Ed Frey and was also survived by many other family and friends. A two time graduate of Miami University, Laura taught kindergarten in the Hamilton City Schools for over 30 years, where she made an impact on countless young lives at Grant Elementary School. She was active in many community organizations in Hamilton, including AAUW, Current Events Club, and her beloved Gourmet Club, which she helped found with many of her closest friends. She was a tireless champion of animals and donations are welcome in her name to Animal Friends Humane Society of Hamilton. Online condolences may be expressed at www.weigelfuneralhome.com. A Memorial Service will be held at the family's convenience.
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text:
IT industry, startups meet Thakur to discuss data protection, tax issues
The meeting was also attended by officials from the Finance Ministry, Electronics & IT Ministry, Department of Telecommunications, CBDT and others.PTI | June 17, 2019, 08:19 IST
Ahead of the Budget, representatives of tech sector and industry bodies like Nasscom, IAMAI and MAIT met Minister of State for Finance Anurag Thakur Saturday to discuss various issues, including tax structure, and share their views on strengthening digital economy. The pre-Budget consultation meeting also focussed on data issues such as use of Big Data technology in improving the forecasting of economic, financial, climatic etc phenomena by analysing large data sets, for providing a fillip to the SME sector and for public governance, as per an official statement.
Other issues, which dominated the discussion, included digital infrastructure and role of government, regulation of digital economy especially in privacy, consumer protection and financial regulation and software as service among others, the statement said.
The meeting was also attended by officials from the Finance Ministry, Electronics & IT Ministry, Department of Telecommunications, CBDT and others.
"The representatives of digital economy and start-ups shared their views and suggestions regarding Big Data, data mining, building of digital infrastructure as the big challenge before Indian economy besides scaling-up and incentivising Research & Development (R&D) within India. While the experts discussed the issues ailing their respective fields, they also suggested a variety of solutions to the sector specific problems," the statement said.
Development of startups and micro, small and medium enterprises (MSME) ecosystem in the country also featured in various suggestions, it added.
The representatives gave suggestions related to angel tax, differential tax structure for assembled and manufactured goods, industry-friendly review of spectrum auction norms, reduction of dependence on international software service, continuation of existing tax benefits to digital companies, reduction in Corporate Tax, among others.
"We urged the government to deal with the angel tax issue urgently. In February, the government had considered Section 56 (2) of the Income Tax but notices have been served under Section 68," Internet and Mobile Association of India President Subho Ray told .
He added that the association also pointed out that a CBDT paper has proposed that for foreign digital companies, their user base also be used as a factor with high weightage among parameters like assets and revenue for profit attribution.
"This, we believe, is not only unfair to foreign digital companies operating in India but fear that the same parameter could be extended to domestic players as well. In a country like India that is rapidly adopting digital platforms, such a move would be a slippery slope for growth," he said.
IT body Nasscom has urged the government to continue the tax incentives to units in special economic zones beyond March 2020, saying that such a move will provide industry with certainty and enable them to invest in long-term strategy.
The body - which represents an industry that earns over USD 130 billion in foreign exchange - has suggested that the new tax-friendly SEZ policy should retain existing tax benefits and provide concessional rate of 9 per cent Minimum Alternative Tax (MAT).
Other participants included representatives from Telecom Equipment Manufacturers Association of India (TEMA), Broadband India Forum, Electronic and Computer Software Export Promotion Council, Manufacturers Association for Information Technology (MAIT); ELCINA (Electronic Industries Association of India), and Indian Cellular and Electronics Association.
Tags : Compliance, Internet, financial regulation, Data Protection, data mining


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'Mr Selfridge' Is Not 'Downton', Producers Say, But They'll Take Its Success: TCA
Diane Haithman is contributing to Deadline's TCA coverage.
Today's TCA panel on PBS' new Masterpiece Classic series Mr. Selfridge — a period drama starring Jeremy Piven as real-life department store entrepreneur Harry Gordon Selfridge — was haunted by the ghost of Downton Abbey. On March 31, the new drama will join the megahit Downton on Sunday nights, so it is not surprising that Masterpiece executive producer Rebecca Eaton announced at the top of the session that the premiere of Mr. Selfridge on Britain's ITV a couple of weeks ago rated higher that the premiere of "that other show."
Related: 'Mr Selfridge' Debuts To Strong Ratings On UK's ITV
Creator Andrew Davies said on the panel that no decision has been made on a second season but "I've already written the first [episodes] of Season 2. We should hear very soon. We've done such good numbers in the UK; I'm very confident." He said that in his mind, he's up for four seasons: "Are you up for it, Jeremy?" he joked. Replied Piven: "I certainly hope I have the job, yeah. To be replaced at this point would be very disappointing. And confusing. It could be like Darrin on Bewitched, I guess."
During the panel that featured Piven, Davies and co-stars Zoe Tapper and Frances O'Connor., Eaton stressed the differences between Selfridge (based on the nonfiction book by Lindy Woodhead) and Downton. She said that while both shows have "gorgeous people, money, life and death, seduction," this story is based on reality. Plus she said, the department store world is a different "precinct beside the country house and the house in town. I don't know why it hasn't been done before."
Related: TV Trailer: 'Mr. Selfridge'
After the panel, she acknowledged that Masterpiece would love to repeat Downton's success with a similar show. "We're adding another member. It's always risky and I think its inevitable that you want to do more of what works," she said. "So when there is a hit show like Downton, I think there's an enormous urge to capitalize on the attention it brings Masterpiece and to build on the audience that we've attracted, this seemed like kind of a perfect fit. But you have to vary it. It's not another country house."
For his part, Piven said after the panel that he felt no competition pressure with Downton. " I feel like I willed this job to happen because I was such a fan of that show", which he said he discovered on Netflix. "They are kind of our lead-in on PBS, our ads run on their show. So they've provided a great deal of help for us, I think there's a great deal of camaraderie there."
Piven was asked about going to period drama after the very contemporary Entourage. He said he was first and foremost attracted to Davies' script: "As I was reading it, I was willing my beard to grow." The actor said he had planned not to go into TV again, but "there was no way I could say no."
Piven grew up in Chicago, ogling the glossy window displays at Marshall Field's. He described getting a role in a Masterpiece series "like telling a Jewish mother you're going to be a doctor. You've made it." He described the real-life Selfridge's rise and fall as akin to Greek tragedy.
Masterpiece Classic


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Places to visit in Jamaica
Visit Montego Bay, Jamaica
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Reggae culture, slave history, dazzling-white beaches and rum distilleries, in Jamaica's second city, Montego Bay.
Set on Jamaica's north coast, Montego Bay is firmly on the map thanks to its white-sand beaches, reggae-infused bars and variety of things to do. Once a hub for the island's sugar industry, it was here that wealthy plantation barons set up home, bringing with them the people they enslaved (a few notable uprisings occurred here over the years). Earlier still, the Spanish used it as a small port for exporting lard ('manteca'), from which its name derives.
Today, you'll find a city of two halves — the glossy resort town you see in brochures and the more frenetic, characterful Mo' Bay that locals know and love. Stroll along the 'Hip Strip' to browse shops, restaurants and bars that bubble with life. Relax on the city's headline beach, Doctor's Cave, with its gently shelving sand and coral reefs. And taste jerk-seasoned barbecued chicken or fish, washed down with a locally made rum on the rocks.
Things to see and do in Montego Bay
The 'Hip Strip' and Downtown
Stretching 3 km (1.9 miles) parallel to the seafront, Gloucester Avenue has been dubbed the 'Hip Strip' by locals thanks to its excitable collection of bars, restaurants, shops and street vendors. Live reggae, hip-hop and soca music drifts from doorways, and there's generally an open, welcoming atmosphere.
You could also get a taste of the city's lively character in the Downtown area, which fans out from the cobbled Sam Sharpe Square. Here, you can drop into pattie shops to try the local delicacy of flaky pastry filled with spiced meat, and visit Gully Market to browse local wares, from bright fabrics to hand-made crafts.
Montego Bay beaches and reefs
Located at the top of the 'Hip Strip' is Doctor's Cave, a 300-m (984-ft) white-gold beach that slopes gently into turquoise waters. While you have to pay to access this beach and it's one of the area's busiest, it's undoubtedly beautiful, as well as being home to a coral reef, a century-old bathing club and various water sports options.
A slightly quieter option is nearby Cornwall Beach, which shares the same quality of sand and clear blue water, as well as being close to some excellent eateries serving fresh fried fish. Or, for a family-friendly option, Walter Fletcher Beach has a go-kart track, tennis courts and water park.
Just off Montego Bay's shore is the Montego Bay Marine Park, an expanse of rainbowed coral reefs and wildlife-rich mangroves. You can head out there to explore the marine life that thrives beneath the waves, either on a scuba-diving trip or a catamaran cruise that stops off at the reefs so you can snorkel among groupers, barracudas and sergeant majors.
Montego Bay's food scene has many culinary influences, with international dishes often infused with traditional Jamaican spices. It won't be hard to come across Jamaica's best-known dish, jerk chicken — the meat is marinaded in a hot sauce and barbecued until crisp. You'll often find jerk seasoning on fish, shrimp and beef, too.
Jamaica's just as well known for its rum, which is usually served on the rocks or as part of a tropical cocktail. You can sip rum at any bar you visit in Montego Bay, but for some context, you could also visit a local rum distillery. Many offer tours that let you look behind the scenes at the rum-making process and tell you about the history of rum making on the island. And, of course, you'll have the option of a tasting or two.
An important sugarcane port between the mid-17th and 20th centuries, Montego Bay has a long history to explore, and not all of it pretty. The wealthy sugar plantation owners enslaved hundreds of Africans, and the city was the site of several slave revolts. The last major uprising, the Christmas Rebellion or Baptist War, took place between 1831 and 1832, and resulted in the public hanging of Samuel Sharpe, the revolt's leader. The city's main square was renamed after him in 1975, and you'll find a monument dedicated to him there.
Also in the square is the Montego Bay Cultural Centre, housed in an early 19th-century building. It's home to the National Museum West and the National Gallery West, where you can browse exhibits focused on Jamaica's history, culture and art. You can also see the Freedom Monument, which pays tribute to the enslaved Africans who fought in the rebellion.
While many of the city's historic buildings have since been destroyed by fires or hurricanes, you can still pay a visit to Rose Hall Great House, an 18th-century Jamaican-Georgian plantation home. It was restored in the 1960s, and you'll see its mahogany floors, wood-panel walls and wooden ceilings. Tours take place throughout the day and tell you about the wealthy Europeans who lived there, as well as the enslaved Africans forced to work in its plantation. There's also the option to tour the house at night to learn about the 'White Witch' spirit said to haunt its halls.
Green Grotto Caves
About an hour's drive east along the coast from Montego Bay, you can descend 12 m (39 ft) into the Green Grotto Caves, a labyrinth of limestone caverns named for the green algae that covers the walls.
A tour will show you some of the unusual formations, stalactites and stalagmites formed within the caves. You'll also hear about the caves' history, with pottery fragments and other artifacts found here indicating they were once used by the indigenous Arawaks. They're also thought to have been used as a hiding place for Spaniards fleeing British invaders during the 17th century, as well as smugglers, and enslaved Africans fleeing their captors.
Look out for bats sleeping on the ceilings and pause to admire the glass-clear lake within one of the caverns, which appeared in 1973 Bond film Live and Let Die.
Best time to visit Montego Bay
Mild enough for the beach at any time of year, Montego Bay experiences warm, dry winters (mid-December to mid-April) and hot, humid summers (June to September), while in-between you'll get a mix of everything with perhaps more rain. Hurricanes are possible in September and October — the wettest months. Visit in July and you might catch reggae Sumfest, the Caribbean's largest music festival, featuring reggae artists from far and wide.
Start planning your tailor-made trip to Montego Bay by contacting one of our Jamaica specialists
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Ocho Rios 56 miles away
Port Antonio 99 miles away
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Hyatt Zilara Rose Hall
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Our specialists seek out authentic ways to get to know the places that could feature in your trip. These activities reflect some of the experiences they've most enjoyed while visiting Montego Bay, and which use the best local guides.
Organic farm tour with alfresco lunch
See a different side to Jamaica on this guided tour of a family-run farm. You'll explore the grounds on foot with your hosts, learning about the local produce, before sitting down to a seven-course plant-based menu out on the terrace.
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text:
package edu.usu.sdl.openstorefront.validation;

import edu.usu.sdl.openstorefront.common.exception.OpenStorefrontRuntimeException;
import java.lang.reflect.Field;
import java.lang.reflect.InvocationTargetException;
import javax.validation.constraints.Size;
import org.apache.commons.beanutils.BeanUtils;

/**
 *
 * @author dshurtleff
 */
public class SizeRule
		extends BaseRule
{

	@Override
	protected boolean validate(Field field, Object dataObject)
	{
		boolean valid = true;

		Size size = field.getAnnotation(Size.class);
		if (size != null) {
			try {
				String value = BeanUtils.getProperty(dataObject, field.getName());
				if (value != null) {
					if (value.length() < size.min()
							|| value.length() > size.max()) {
						valid = false;
					}
				} else {
					if (size.min() > 0) {
						valid = false;
					}
				}
			} catch (IllegalAccessException | InvocationTargetException | NoSuchMethodException ex) {
				throw new OpenStorefrontRuntimeException("Unexpected error occur trying to get value from object.", ex);
			}
		}
		return valid;
	}

	@Override
	protected String getMessage()
	{
		return "Value is out of range";
	}

	@Override
	protected String getValidationRule(Field field)
	{
		StringBuilder sb = new StringBuilder();
		Size size = field.getAnnotation(Size.class);
		sb.append("Min Length: ").append(size.min()).append(" Max Length: ").append(size.max());
		return sb.toString();
	}

}

redpajama_set_name: RedPajamaGithub


text:
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Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters
Timothy J. Nott ORCID: orcid.org/0000-0002-5210-49311,
Timothy D. Craggs ORCID: orcid.org/0000-0002-7121-06092 &
Andrew J. Baldwin ORCID: orcid.org/0000-0001-7579-88441
Nature Chemistry volume 8, pages569–575(2016)Cite this article
Biophysical chemistry
Nuclear organization
Membraneless organelles are cellular compartments made from drops of liquid protein inside a cell. These compartments assemble via the phase separation of disordered regions of proteins in response to changes in the cellular environment and the cell cycle. Here we demonstrate that the solvent environment within the interior of these cellular bodies behaves more like an organic solvent than like water. One of the most-stable biological structures known, the DNA double helix, can be melted once inside the liquid droplet, and simultaneously structures formed from regulatory single-stranded nucleic acids are stabilized. Moreover, proteins are shown to have a wide range of absorption or exclusion from these bodies, and can act as importers for otherwise-excluded nucleic acids, which suggests the existence of a protein-mediated trafficking system. A common strategy in organic chemistry is to utilize different solvents to influence the behaviour of molecules and reactions. These results reveal that cells have also evolved this capability by exploiting the interiors of membraneless organelles.
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Figure 1: Membraneless organelles selectively partition oligonucleotides.
Figure 2: Ddx4N1 organelles destabilize oligonucleotide duplexes.
Figure 3: Ddx4N1 organelle partitioning depends on the stability of the nucleic acid structure.
Figure 4: Ddx4N1 organelles differentially partition proteins, which in turn can act as importers for dsDNA.
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Nizami, Z., Deryusheva, S. & Gall, J. G. The Cajal body and histone locus body. Cold Spring Harb. Perspect. Biol. 2, 653 (2010).
Anderson, P. & Kedersha, N. RNA granules post-transcriptional and epigenetic modulators of gene expression. Nature Rev. Mol. Cell Biol. 10, 430–436 (2009).
Brangwynne, C. P. et al. Germline P granules are liquid droplets that localize by controlled dissolution/condensation. Science 324, 1729–1732 (2009).
Brangwynne, C. P., Mitchison, T. J. & Hyman, A. A. Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes. Proc. Natl Acad. Sci. USA 108, 4334–4339 (2011).
Berry, J., Weber, S. C., Vaidya, N., Haataja, M. & Brangwynne, C. P. RNA transcription modulates phase transition-driven nuclear body assembly. Proc. Natl Acad. Sci. USA 112, E5237–E5245 (2015).
Nott, T. J. et al. Phase transition of a disordered nuage protein generates environmentally responsive membraneless organelles. Mol. Cell 57, 936–947 (2015).
Li, P. L. et al. Phase transitions in the assembly of multivalent signalling proteins. Nature 483, 336–129 (2012).
Elbaum-Garfinkle, S. et al. The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics. Proc. Natl Acad. Sci. USA 112, 7189–7194 (2015).
Weber, S. C. & Brangwynne, C. P. Inverse size scaling of the nucleolus by a concentration-dependent phase transition. Curr. Biol. 25, 641–646 (2015).
Patel, A. et al. A liquid-to-solid phase transition of the ALS protein FUS accelerated by disease mutation. Cell 162, 1066–1077 (2015).
Lin, Y., Protter, D. S., Rosen, M. K. & Parker, R. Formation and maturation of phase-separated liquid droplets by RNA-binding proteins. Mol. Cell 60, 208–219 (2015).
Molliex, A. et al. Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization. Cell 163, 123–133 (2015).
Jiang, H. et al. Phase transition of spindle-associated protein regulate spindle apparatus assembly. Cell 163, 108–122 (2015).
Burke, K. A., Janke, A. M., Rhine, C. L. & Fawzi, N. L. Residue-by-residue view of in vitro FUS granules that bind the C-terminal domain of RNA polymerase II. Mol. Cell 60, 231–241 (2015).
Swedlow, J. R. & Lamond, A. I. Nuclear dynamics: where genes are and how they got there. Genome Biol. 2, 2 (2001).
Meikar, O., Da Ros, M., Liljenback, H., Toppari, J. & Kotaja, N. Accumulation of piRNAs in the chromatoid bodies purified by a novel isolation protocol. Exp. Cell Res. 316, 1567–1575 (2010).
Han, T. N. W. et al. Cell-free formation of RNA granules: bound RNAs identify features and components of cellular assemblies. Cell 149, 768–779 (2012).
Kato, M. et al. Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell 149, 753–767 (2012).
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McNaughton, B. R., Cronican, J. J., Thompson, D. B. & Liu, D. R. Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins. Proc. Natl Acad. Sci. USA 106, 6111–6116 (2009).
Thiam, A. R., Farese, R. V. & Walther, T. C. The biophysics and cell biology of lipid droplets. Nature Rev. Mol. Cell Biol. 14, 775–786 (2013).
A.J.B. thanks the Biotechnology and Biological Sciences Research Council (BBSRC grant BB/J014346/1) for a David Philip's fellowship, and T.D.C. thanks the BBSRC for funding. T.J.N. thanks the BBSRC and New College for funding and O. Tkachenko, G. Hochberg, J. Hopper, S. Chandler and S. Habash for gifts of GFP-tagged proteins, E Fussner-Dupas for antibody-labelling support and B. Davis for discussion and the use of instrumentation. The authors thank L. E. Kay for discussion.
Physical and Theoretical Chemistry, University of Oxford, Oxford OX1 3QZ, UK
Timothy J. Nott
& Andrew J. Baldwin
DNA–Protein Interactions Unit, School of Biochemistry, University of Bristol, Bristol BS8 1TD, UK
Timothy D. Craggs
Search for Timothy J. Nott in:
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T.J.N., A.J.B. and T.D.C. conceived and designed the experiments; T.J.N. and A.J.B. analysed data and wrote the manuscript with input from T.D.C.; T.J.N. performed the experiments.
Corresponding authors
Correspondence to Timothy J. Nott or Andrew J. Baldwin.
The authors declare no competing financial interests.
Supplementary information (PDF 2770 kb)
Nott, T., Craggs, T. & Baldwin, A. Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters. Nature Chem 8, 569–575 (2016) doi:10.1038/nchem.2519
Issue Date: June 2016
DOI: https://doi.org/10.1038/nchem.2519
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