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The human nuclear factor related to kappa-B-binding protein (NFRKB) is a 1299-residue protein that is a component of the metazoan INO80 complex involved in chromatin remodeling, transcription regulation, DNA replication and DNA repair. Although full length NFRKB is predicted to be around 65% disordered, comparative sequence analysis identified several potentially structured sections in the N-terminal region of the protein. These regions were targeted for crystallographic studies, and the structure of one of these regions spanning residues 370–495 was determined using the JCSG high-throughput structure determination pipeline. The structure reveals a novel, mostly helical domain reminiscent of the winged-helix fold typically involved in DNA binding. However, further analysis shows that this domain does not bind DNA, suggesting it may belong to a small group of winged-helix domains involved in protein-protein interactions.
Funding: This work was supported by the National Institutes of Health, National Institute of General Medical Sciences, Protein Structure Initiative grants U01 GM094614 to Robert Fletterick and U54 GM094586 to the Joint Center for Structural Genomics (JCSG). The Stanford Synchrotron Radiation Lightsource (SSRL) Structural Molecular Biology Program is supported by the DOE Office of Biological and Environmental Research, and by the National Institutes of Health, National Institute of General Medical Sciences (including P41GM103393) and the National Center for Research Resources (P41RR001209). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
The INO80 complex is a universally conserved multi-subunit protein complex anchored around the Snf2 family ATPase (INO80 protein), and is involved in several DNA-related functions including chromatin remodeling, transcription regulation, replication, and DNA repair –. The mammalian INO80 complex is composed of three protein modules , one of which consists of proteins specific to metazoa (animals), and not involved in ATP-dependent nucleosome remodeling. Nuclear factor related to kappa-B-binding protein (NFRKB) is a part of this module and modulates the deubiquitinase activity of UCHL5 in the INO80 complex . Recent genome-wide RNAi screening revealed that NFRKB has an important function in the acquisition of pluripotency of human cells . NFRKB enhances induced pluripotent stem cell generation and knockdown of the NFRKB gene affects the reprogramming process leading to a reduced number of human induced pluripotent stem (iPS) cell colonies.
The human NFKRB protein (Uniprot ID Q6P4R8), also known as subunit G of the INO80 complex, consists of 1299 amino acids. This subunit is responsible for DNA binding with a consensus sequence of 5′-GGGGAATCTCC-3′ . The structure of the full-length NFRKB has yet to be determined, most likely due to the challenges of crystallizing such a large protein with numerous domains and predicted disordered regions. NFRKB is predicted to be 65% disordered, with the longest disordered segment (650aa) spanning the entire C-terminal half of the protein. However, at least three structured domains are predicted in the N-terminal half of NFRKB, and could be amenable to X-ray structural studies . Based on PsiPred and Disopred sequence analyses, we made 16 constructs that covered various possible boundaries of these three putative domains, and one construct, residues 370–495, produced diffraction-quality crystals. The structure of this domain was determined to 2.18 Å resolution and reveals a novel helical domain (NFRKB_WHL) bearing remarkable similarity to a winged-helix domain, usually associated with DNA binding. Based on our structural findings, this domain was subsequently used to seed a new NFRKB winged-helix-like PFAM  family (PF14465).
The crystal structure of the human NFRKB_WHL domain (residues 370–495) consists of two protomers (residues 372–483 in chain A and 370–483 in chain B), one sodium ion and 25 water molecules in the crystallographic asymmetric unit. The last 12 residues of the construct (residues 484–495) were disordered and were not modeled. In addition, residues 370–371, 463–465 in chain A and 440–443, 462–463 in chain B were not modeled due to poor electron density. The Matthews' coefficient (VM)  and the estimated solvent content are 1.84 Å3/Da and 33.1% respectively. The Ramachandran plot produced by MolProbity  shows that 97.5% of the residues are in favored regions with no outliers.
The NFRKB_WHL structure adopts a fold similar to the winged-helix DNA binding fold, comprising of four α-helices, three 310-helices and a short β-sheet composed of three β-strands (Figure 1).
Figure 1. Crystal structure of the winged-helix domain of human NFRKB.
(A) Ribbon diagram of the human NFRKB_WHL domain (residues 370–495) structure is color-coded from N-terminus (blue) to C-terminus (red). Helices α1–α4, β-strands β1–β3 and 310 helixes η1–η3 are indicated. The dashed line between β3 and α4 corresponds to three disordered residues that were omitted from the model. (B) Protein sequence of the NFRKB_WHL domain annotated with the corresponding secondary structure elements. The dashed lines indicate residues that were in the construct, but are not in the refined model due to lack of interpretable electron density. Figure 1B was prepared with ESPript .
A search for structurally similar proteins using the DALI server  returned about 300 hits with Z-scores above 5.0, with a maximum score of only 7.5. Most of the matches were to DNA binding domains within large, multidomain, proteins, with a few low scoring matches to proteins involved in protein-protein interactions. The closest match was to the DNA binding domain of the virulence gene activator AphA protein from Vibrio cholerae (PDB code 1yg2 ) with a Z-score of 7.5 and RMSD of 3.0 Å over 67 residues. This structure, however, was determined without DNA and, therefore, does not provide any insights on the potential role of NFRKB _WHL in DNA binding.
In order to investigate whether NFRKB_WHL might interact with DNA, 45 of the top DALI hits that had bound DNA in their structures were superimposed onto the NFRKB_WHL structure to identify regions potentially involved in DNA binding (Figure 2). Further analysis of the superimposed structures revealed two types of DNA binding modes. The first group includes structures where the main recognition helix (α3 in NFRKB_WHL) binds in the major groove of the bound DNA (PDB codes 2d45, 1sax, 1u8r, 1xsd, 2xro, 3co7) (Figure 2). The second group includes structures with Z-DNA bound to proteins (PDB codes 1j75, 2gxb, 2heo, 3eyi) where there is limited interaction between protein and DNA (Figure 3).
Figure 2. Superposition of NFRKB_WHL domain structure with the MecI repressor structure from Staphylococcus aureus (PDB code 2d45).
Helix α3 of the NFRKB_WHL domain (magenta) is located in the major groove of the DNA upon superposition onto the MecI repressor (cyan, protein and orange, DNA backbone). Helix α4 of NFRKB_WHL does not map to any corresponding helix in MecI repressor and clashes with the DNA.
Figure 3. Superposition of NFRKB_WHL domain onto the DNA-binding domain Zα of DLM-1 (PDB code: 1j75).
The DNA binding domain Zα of DLM-1 (green) binds a left-handed Z-DNA (orange backbone) and has limited interactions with the DNA. The human NFRKB_WHL domain is shown in magenta with α3 and α4 helices labeled.
In both cases, however, the residues interacting with DNA are not conserved in NFRKB_WHL. A structure-based sequence alignment of helix α3 shows a clear lack of conserved residues (Figure 4), with the exception of two hydrophobic residues that point toward the hydrophobic core of the protein and are likely involved in stabilizing the interaction and orientation of this helix on the protein. The sequence of this helix lacks any basic residues, thereby making it unlikely to interact with DNA. Therefore, despite the structural similarity to winged-helix DNA binding domains, NFRKB_WHL is unlikely to bind DNA. The calculated isoelectric point of 4.3 of this domain is also not favorable for DNA binding. In addition, results of Differential Scanning Fluorimetry (DSF) experiments to test whether there is any change in stability of NFRKB_WHL in the presence of consensus DNA (5′-GGGGAATCTCC-3′) further support the observation that NFRKB_WHL may not bind DNA. The protein's melting temperature remains unchanged upon mixing with DNA for different ratios of protein:DNA (Figure 5, Table 1), indicating that the DNA tested does not stabilize NFRKB_WHL.
Figure 4. Structure-based sequence alignment of helix α3.
Helix α3 of NFRKB_WHL was aligned with the corresponding helices from some of the structurally similar proteins based on DALI alignment. Only two hydrophobic residues of NFKRB (427 V and 431 L; blue-grey background) exhibit some degree of conservation. Residues interacting with DNA in other structures are colored (polar and aromatic residues in pink and basic residues in blue).
Figure 5. Lack of DNA binding to the NFRKB winged helix domain.
Comparison of the DSF melting curves of NFRKB winged helix domain in the absence (solid line) and presence of different concentrations of DNA (dashed and dotted lines). The fluorescence of the dye was monitored as a function of temperature. The melting temperature of the protein correlates to amount of binding of the fluorescent dye to the protein as it unfolds. The curves have been normalized setting the maximal/minimal fluorescence response as 0% to 100% protein unfolding.
Table 1. Effect of DNA on the thermal unfolding of the NFRKB winged helix domain.
Interestingly, one of the higher scoring structural matches identified in the DALI search is a winged-helix domain from the yeast (PDB code 1ldd) and human (PDB code 1ldj) anaphase-promoting complex. NFRKB_WHL has a significant structural similarity (Dali Z-score of 6.7) to one of the winged-helix motifs within the C-terminal domain (CTD) of the cullin protein portion of the of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex. This domain follows three repeats of the cullin repeat and is involved in binding of the RING finger protein Rbx1 . NFRKB_WHL and the winged-helix subdomain of CTD superpose with a RMSD of 2.15 Å over 63 residues (Figure 6) and share a sequence identity of 10%. Thus, it is possible that NFRKB_WHL may be involved in protein-protein interactions rather than DNA binding.
Figure 6. Comparison of NFRKB_WHL domain with the Cul1 domain of the yeast anaphase-promoting complex.
Superposition of NFRKB_WHL (green) onto the cullin-homology domain (Cul1) of the anaphase-promoting complex (pdb code: 1ldd, orange) in shown in ribbon representation, with their N- and C-termini labeled. The last helix, α4, in the NFRKB_WHL does not have a counterpart in 1ldd.
Initial sequence analysis of this domain, including a sequence search of the PFAM database , did not identify any similarity to other winged-helix DNA binding domains. In fact, none of the fold prediction or distant homology recognition tools yielded any statistically significant matches to any characterized protein family. Thus, the NFRKB_WHL structure formed the basis for a new Pfam domain, PF14465. This new domain was thereafter identified in all animal genomes, as well as several single cell eukaryotes. It is always found in proteins bearing overall homology to NFRKB.
We have determined the structure of a predicted ordered domain from the human NFRKB protein at 2.18 Å resolution. The identification of this domain was based on PsiPred and Disopred sequence analysis that indicated the existence of a structurally ordered region bordered by disordered/low complexity regions. The crystal structure of this domain unexpectedly revealed similarity to winged-helix DNA binding domains in structures, such as MecI repressor (PDB code 2d45; ) and Zα domain of DLM-1 (PDB code 1j75; ). However, the lack of sequence similarity between this domain and other winged-helix DNA binding domains, the absence of any observable protein-DNA interaction in a DSF experiment, and the lack of positively charged residues in the putative DNA binding helix α3, indicate that this domain likely does not bind DNA. However, similarity to the C-terminal domain in cullins, which is involved in binding of other members of the Skp, Cullin, F-box containing (SCF) complex, suggest a possible role in protein-protein interactions. The NFRKB_WHL domain is the founding member of a new Pfam winged-helix-like family, (NFRKB_winged; PF14465; http://pfam.sanger.ac.uk/family/PF14465), which currently contains 39 sequences from 33 species.
The protein encoded by the human NFRKB gene (Uniprot id: Q6P4R8) did not have any domain annotation in the PfamA database (release 25.0) , and fold recognition algorithms (HHPred  and FFAS ) did not yield any significant domain predictions. However, secondary structure and structural disorder predictions calculated with PsiPred  and Disopred , respectively, suggested that the N-terminal half of this protein likely contained up to three domains with well-defined, three-dimensional structure. Since these predictions in general do not provide reliable domain boundaries, 16 constructs corresponding to predicted ordered regions were selected (1–208, 1–225, 1–275, 1–307, 1–486, 1–495, 1–651, 1–710, 335–495, 335–585, 357–485, 366–485, 370–495, 370–585, 503–651, 503–710) for expression and crystallization trials. The construct consisting of residues 370–495 yielded diffracting crystals.
Clones were generated using the Polymerase Incomplete Primer Extension (PIPE) cloning method . The gene encoding RF2003A.NFRKB (UniProt: Q6P4R8) was amplified by polymerase chain reaction (PCR) using a Homo sapiens cDNA from the Mammalian Gene Collection (MGC) as template, PfuTurbo DNA polymerase (Stratagene) and I-PIPE (Insert) primers (forward primer, 5′-ctgtacttccagggcCTTGGAATCAATGAAATATCTTCCAGC -3′; reverse primer, 5′-aattaagtcgcgttaTGAGCTGTCTTCATTTTCTTGCTTACAG-3′, target sequence in upper case) that included sequences for the predicted 5′ and 3′ ends of the full length construct. The expression vector, pSpeedET, which encodes an amino-terminal tobacco etch virus (TEV) protease-cleavable expression and purification tag (MGSDKIHHHHHHENLYFQ/G), was PCR amplified with V-PIPE (Vector) primers (forward primer: 5′-taacgcgacttaattaactcgtttaaacggtctccagc-3′, reverse primer: 5′-gccctggaagtacaggttttcgtgatgatgatgatgatg-3′). V-PIPE and I-PIPE PCR products were mixed to anneal the amplified DNA fragments together. Escherichia coli GeneHogs (Invitrogen) competent cells were transformed with the I-PIPE/V-PIPE mixture and dispensed on selective LB-agar plates. The cloning junctions were confirmed by DNA sequencing. Using the PIPE method, the gene segment encoding residues M1-C369 and D496-Q1299 were deleted. Expression was performed in a selenomethionine-containing medium at 37°C. Selenomethionine was incorporated via inhibition of methionine biosynthesis , which does not require a methionine auxotrophic strain. At the end of fermentation, lysozyme was added to the culture to a final concentration of 250 µg/ml, and the cells were harvested and frozen. After one freeze/thaw cycle, the cells were homogenized and sonicated in lysis buffer [40 mM Tris, 300 mM NaCl, 10 mM imidazole, 1 mM Tris(2-carboxyethyl)phosphine-HCl (TCEP), pH 8.0]. The lysate was clarified by centrifugation at 32,500× g for 30 minutes. The soluble fraction was passed over nickel-chelating resin (GE Healthcare) pre-equilibrated with lysis buffer, the resin washed with wash buffer [40 mM Tris, 300 mM NaCl, 40 mM imidazole, 10% (v/v) glycerol, 1 mM TCEP, pH 8.0], and the protein was eluted with elution buffer [20 mM Tris, 300 mM imidazole, 10% (v/v) glycerol, 150 mM NaCl, 1 mM TCEP, pH 8.0]. The eluate was buffer-exchanged with TEV buffer [20 mM Tris, 150 mM NaCl, 30 mM imidazole, 1 mM TCEP, pH 8.0] using a PD-10 column (GE Healthcare), and incubated with 1 mg of TEV protease per 15 mg of eluted protein for 2 hr at ambient temperature followed by overnight incubation at 4°C. The protease-treated eluate was passed over nickel-chelating resin (GE Healthcare) pre-equilibrated with crystallization buffer [20 mM Tris, 150 mM NaCl, 30 mM imidazole, 1 mM TCEP, pH 8.0] and the resin was washed with the same buffer. The flow-through and wash fractions were combined and concentrated to 21.3 mg/ml by centrifugal ultrafiltration (Millipore) for crystallization trials. Lysine residues were reductively methylated by adding 40 µl 0.98 M dimethylaminoborane and 80 µl 3.26% by weight formaldehyde, per milliliter of protein, over 2 hours in the presence of crystallization buffer at 277 K . Methylation reagents were subsequently removed using a PD-10 column and the protein was concentrated to 26.7 mg/ml using ultrafiltration. The NFRKB_WHL construct was crystallized using the nanodroplet vapor diffusion method  with standard JCSG crystallization protocols . Sitting drops composed of 100 nl protein solution mixed with 100 nl crystallization solution were equilibrated against a 50 µl reservoir at 277 K for 22 days prior to harvest. The crystallization solution consisted of 0.09 M HEPES pH 7.5, 10% glycerol, 1.26 M tri-sodium citrate. Ethylene glycol was added to a final concentration of 10% (v/v) as a cryoprotectant. Initial screening for diffraction was carried out using the Stanford Automated Mounting system (SAM)  at the Stanford Synchrotron Radiation Lightsource (SSRL, Menlo Park, CA). The complementary DNA from Homo sapiens (MGC Number 71524) was obtained from Invitrogen (Mammalian Gene Collection).
Multi-wavelength anomalous diffraction (MAD) data were collected to 2.18 Å resolution at wavelengths corresponding to inflection (0.97936 Å), peak (0.97915 Å), and high energy remote (0.91837 Å) of the Selenium edge at beam line BL9-2 at SSRL. The data sets were collected at −173°C using a MAR325 CCD detector and the BLU-ICE data collection environment . The data were processed with XDS  and scaled with XSCALE  in space group P43212. Phasing was performed with SHELXD  and autoSHARP  which resulted in a mean figure of merit of 0.18 with one selenium site per protein chain. Automatic model building was performed with RESOLVE . Model completion and refinement were performed with COOT  and REFMAC 5.6.0116  using the high energy remote wavelength data. The refinement included experimental phase restraints in the form of Hendrickson-Lattman coefficients from SHARP, NCS restraints, and TLS refinement with two TLS groups per chain. Data collection and refinement statistics are summarized in Table 2.
Table 2. Crystallographic data and refinement statistics for NFRKB (PDB code 3u21).
The Differential Scanning Fluorimetry (DSF) experiment used to measure the effect of DNA binding on protein stability was performed at room temperature using a MxPro3005P PCR instrument (Stratagene). The optimized reaction mixture contained 10 µM of NFRKB (aa 370–495), 20 µM, 80 µM or 160 µM DNA (GGGGAATCTCC; the consensus sequence for human NFRKB, Uniprot Id Q6P4R8) and 1× SYPRO Orange protein gel stain (Invitrogen) in the assay buffer (20 mM Tris-HCl pH 8.0, 10% glycerol, 5 mM DTT, 150 mM NaCl). The DNA oligonucleotides were purchased from Integrated DNA technologies. Each experiment was performed in triplicate in 96 well polypropylene plates (Agilent Technologies) by adding 20 µl of protein/DNA mixture to 30 µl of dye/buffer mixture. The reactions were mixed, centrifuged and incubated for 30 min at 4°C. For thermal stability measurements of the protein, the fluorescence of the dye was followed as a function of time using a FRROX filter set with an excitation wavelength of 492 nm and an emission wavelength of 610 nm. Data were collected from 25° to 95°C at 1°C/30 s intervals, and plotted to calculate the melting temperature of the protein (Figure 5, Table 1).
The quality of the crystal structure was analyzed using the JCSG Quality Control server (http://smb.slac.stanford.edu/jcsg/QC/). This server verifies the stereochemical quality of the model using AutoDepInputTool , MolProbity , WHATIF , RESOLVE , as well as several in-house scripts, and summarizes the outputs. Protein quaternary structure analysis was carried out using the PISA server . Figures were prepared with PyMOL . Atomic coordinates and experimental structure factors have been deposited in the PDB and are accessible under the code 3u21.
We thank the members of the JCSG high-throughput structural biology pipeline for their contribution to this work. Portions of this research were carried out at the Stanford Synchrotron Radiation Lightsource (SSRL), a Directorate of SLAC National Accelerator Laboratory and an Office of Science User Facility operated for the U.S. Department of Energy Office of Science by Stanford University. The contents of this publication are solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences, the National Center for Research Resources, or the National Institutes of Health.
Conceived and designed the experiments: AK SM FY LJ AMD AG SAL BRC RF IAW. Performed the experiments: AK CF FY PN CW. Analyzed the data: AK LJ CF CW AG. Wrote the paper: AK AMD FY MAE. Performed key roles in the Joint Center for Structural Genomics (JCSG) structure genomics pipeline, including bioinformatics, protein cloning and purification, crystallization, diffraction screening, and structure validation and deposition: LJ HJC CF MAE HEK. JCSG Core leaders: AMD AG SAL. Principal Investigator of the JCSG: IAW. PI and co-PI on the PSI:Biology "Structures of Protein Complexes Regulating Transcription in Embryonic Stem Cells" project, respectively: RF BRC.
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34. Emsley P, Lohkamp B, Scott WG, Cowtan K (2010) Features and development of Coot. Acta Crystallogr Sect D Biol Crystallogr 66: 486–501.
35. Winn MD, Murshudov GN, Papiz MZ (2003) Macromolecular TLS refinement in REFMAC at moderate resolutions. Methods Enzymol 374: 300–321.
36. Yang H, Guranovic V, Dutta S, Feng Z, Berman HM, et al. (2004) Automated and accurate deposition of structures solved by X-ray diffraction to the Protein Data Bank. Acta Crystallogr Sect D Biol Crystallogr 60: 1833–1839.
37. Vriend G (1990) WHAT IF: a molecular modeling and drug design program. J Mol Graph 8: 52–56, 29.
38. Krissinel E, Henrick K (2007) Inference of macromolecular assemblies from crystalline state. J Mol Biol 372: 774–797.
39. Schrodinger LLC (2010) The PyMOL Molecular Graphics System, Version 1.3r1.
40. Gouet P, Courcelle E, Stuart DI, Metoz F (1999) ESPript: analysis of multiple sequence alignments in PostScript. Bioinformatics 15: 305–308.
41. Diederichs K, Karplus PA (1997) Improved R-factors for diffraction data analysis in macromolecular crystallography. Nat Struct Biol 4: 269–275.
42. Collaborative Computational Project Number 4 (1994) The CCP4 suite: programs for protein crystallography. Acta Crystallogr Sect D Biol Crystallogr 50: 760–763.
43. Cruickshank DW (1999) Remarks about protein structure precision. Acta Crystallogr Sect D Biol Crystallogr 55: 583–601.


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Why Everything Old Is New Again
It's hard to tell what year / decade we're currently in. Roger Federer won the Australian Open and is number one in the world, there's Nokia phones in the market and people playing Snake, Star Wars topped the box office and shows like Will & Grace, Roseanne, Murphy Brown are back on TV screens or planning to be.
For those old enough, you might remember the controversy in 1992 that surrounded Murphy Brown when the lead female, 40+ character fell pregnant causing outrage to the then Vice President of the United States who publically condemned her and her morals for deciding to raise the child as a single parent. I mean, could you see in 2018 a leader in a position of power condemning minority group(s) and doing so publically, say on Twitter? Doubt it will happen but regardless, the trend towards nostalgia marketing is evident and is appealing to the masses.
There's a number of reasons for its success. Nostalgia marketing relies on the memories of people and their younger self – a time without the children, the work responsibilities, the mortgage repayments, the day to day grind. Life was simple, it was easier, and you can think back to these as fond times. We can be more optimistic when we remember the 'good old days' and the products, brands or services that mattered to us. It reconnects us emotionally – which brings us closer to the brand.
It also provides functional benefits as demonstrates the brands making these products or services have longevity and we can celebrate that what they did in the past still applies in the modern world. We believe that history repeats itself so can applaud a brand for recognising how to reinvent the wheel without deviating too far from what we originally valued. There's a sense of pride that our children are now the Star Wars fans and we can share it with them.
From a brand perspective, nostalgia can also encourage conversation and advocacy … customers are caught up on their emotions and more likely to talk to each other about the nostalgia they're feeling – anyone been recommended to watch Stranger Things recently? Think about the pledge to 'Make America Great Again' – it infers there was a past time where things were better in America and gets people talking about finding ways to return to such a time.
How Murphy Brown 2.0 will go against 2018 politicians in the White House remains to be seen but next time you find yourself all nostalgic – you might know why.
Don't Change The Channel (Or The Message)
Stumped to Find A Solution To Bail Australia Out

redpajama_set_name: RedPajamaCommonCrawl


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text:
Fired Arizona Social Services Chief Had Own Police Force, Armory
Tim Jeffries had to be disarmed (without incident) before he was walked out of the building.
Wait until you read about this Arizona Republican who until recently was the head of the Arizona Department of Economic Security.
Timothy Jeffries allegedly did the following:
Engaged in mass firings at DES, calling managers he didn't like "bullies" and encouraging other people in his department to "exit" staff that didn't toe his line.
Fired those employees only after "persuading" them to relinquish their state job protections.
Created a 'no-hire list' to keep his enemies from getting other state jobs.
Held a staff party at which Jeffries used state money to pay for alcohol for employees, allegedly during work hours.
Gave a no-bid consulting contract to a personal friend without review.
AZ Central adds:
Critics also said Jeffries pushed the limits by having his public-relations staff produce "mojo" videos of him that he sent frequently via email to DES employees, whose computers were programmed to not function until the entire video was watched, according to DES employees and a contractor who said he programmed the video player. He also sent out emails to DES staff that promoted his faith and political views.
Here's more on the pushing his religion thing:
And then... there's the GUNS AND AMMO:
State troopers confiscated about 50 handguns and 80,000 rounds of ammunition stored in the Arizona Department of Economic Security's basement shortly after Director Tim Jeffries — who had expanded the agency's armed police force — was forced to resign and escorted from the building...
Also ejected from their jobs and the building were three of Jeffries's managers, all of whom had state-purchased semiautomatic handguns.
Jeffries claims his use of state funds to purchase guns and arm a personal police force for his office -- which, by the way, is also the welfare and food stamp office -- was inspired by the attack on a social service agency in San Bernadino, and that he, Jeffries, was well within his rights to take it into his own hands to protect his office from "jihadists."
And finally, the Governor had to protect his office from him.
The state of Arizona is lucky they ejected this guy from office (and disarmed him and his team) before a tragedy occurred.
I didn't mention what political party Jeffries belongs to because I don't have to, do I? Both sides don't.
PS. The Governor has opened a hotline for employees fired by Jeffries to appeal to have their jobs back. The Office of Administration reported that since 4pm Monday they have received SEVENTY ONE phone calls from people fired by Jeffries.
Arizona, Guns, Timothy Jeffries
He Wanted 'Any Reason To Shoot Us': Cop Holds Latino Ex-state Senator At Gunpoint For Honking
A former Arizona state senator explained this week how a police officer threatened to kill him after he honked at an unmarked van to avoid a traffic accident.
Arkansas School Arms Teachers
When the new school year begins in Clarksville, Arkansas, some teachers will carry a slightly heavier workload - 9mm handguns. The school district is making use of a state law that allows teachers to carry concealed handguns on campus.
By Diane Sweet
With DHS Position, Clarke Would Be The First 'Patriot' Leader To Hold A Federal Post
Milwaukee sheriff's continuing links with extremist 'constitutionalists' raises concerns about his potential role as Trump's federal liaison to local law enforcement.
By David Neiwert
Dear Liberty U Lawyers: Guns On Campus Will Cost You Money
Liberty University will be allowing handguns in residence halls next fall. Are their insurance companies on board with this? When a gun accident happens they have to pay.


text:
Nel 1986 ha preso parte alla missione STS-61-C a bordo dello Space Shuttle Columbia come specialista del carico di bordo.

Note

Altri progetti

Collegamenti esterni

redpajama_set_name: RedPajamaWikipedia


text:
Google to launch Pixel smartwatch in 2022
Renders show the Pixel watch as having a round face with no bezel, as well as a heart rate monitor and step counting.
US-based tech giant Google is reportedly working on its own in-house smartwatch that's set to launch in 2022.
According to a new report from Insider, the Pixel watch code-named Rohan is in the works. Renders show the Pixel watch as having a round face with no bezel, as well as a heart rate monitor and step counting.
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The device is expected to cost more than Fitbit's line of products and could compete with the Apple Watch line of devices.
Google apparently wants to ensure that the wearable would be comfortable to wear for the majority of the population, which means it might come in various sizes.
Also Read : Here's everything about the Huawei GT 2
The watch will have basic fitness tracking features, including step counting and a heart rate monitor, with Google also reportedly working on debuting a Fitbit integration into Wear OS with the new watch when it launches.
Google was earlier expected to unveil its first smartwatch alongside the Pixel 6, but the launch was reportedly delayed.
Google apparently wants to ensure that the wearable would be comfortable to wear for the majority of the population, which means it might come in various sizes.Unsplash
Meanwhile, Google has started rolling out its Camera app version 8.4 to older Pixel phones, bringing camera features from Pixel 6 and 6 Pro.
The update includes a few new features, including a Timer light, a manual white balance toggle, and a manual exposure toggle. The new version of the App has added a gear icon next to the "V" icon in the top drop-down menu. (IANS/SP)
(Keywords : Google, tech, smartwatch, pixel, device, costly, comfortable, fitness tracker, steps, heart rate.)
Realme Unveils New "6 Series" Smartphones in India Starting From ... ›
Smartwatches | Shop Fitbit ›
Best smartwatch 2021: the top wearables you should buy today ... ›
This Smartwatch-based Monitoring System May Help Patients With Parkinson's Disease
Parkinson's disease is marked by a breakdown of involuntary movement (dyskinesia) and the appearance of tremors, which severely detract from a patient's quality of life. Unsplash
A monitoring system based on commercial smartwatches has been developed that can detect movement issues and tremors in patients with Parkinson's disease, researchers say.
The findings, published in the journal Science Translational Medicine, suggest the platform could allow clinicians to remotely monitor the progression of a patient's condition and adjust medication plans accordingly to improve outcomes.
Parkinson's disease is marked by a breakdown of involuntary movement (dyskinesia) and the appearance of tremors, which severely detract from a patient's quality of life.
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"These symptoms can be treated with medications, but patients respond best if clinicians can precisely titrate and change drug regimens to match the severity of symptoms," said the researchers, including one of Indian-origin Adeeti V. Ullal, Senior Manager, Motion Health Technologies, Apple.
"However, clinicians currently base their assessments of patients on infrequent clinical visits that fail to capture subtle changes in symptoms," they added.
The team tackled this issue with their Motor fluctuations Monitor for Parkinson's Disease (MM4PD) — a collection of algorithms that uses smartwatch sensors to capture daily fluctuations in the wearer's movement patterns.
A monitoring system based on commercial smartwatches has been developed that can detect movement issues and tremors in patients with Parkinson's disease, researchers say. Unsplash
The system identified patterns in the severity of tremors and dyskinesia over six months when worn by 225 patients and detected changes in symptoms that clinicians noted might be missed in traditional evaluations.
Furthermore, MM4PD recorded signs of emerging tremors and impairments, which may require changes in medication schedules to treat properly, the team said.
ALSO READ: Transforming The Face Of Slums In Delhi
For the study, the system was tested in a study involving 343 patients — including 225 who the researchers followed for six months.
The system gave evaluations that matched a clinician's estimates in 94 percent of the subjects. (IANS)
Tech Review (Realme Watch S Pro): Convenient and Affordable Smartwatch Under 10K
The Realme Watch S is priced at Rs 4,999 in India, while the Realme Watch S Pro's price is set at Rs 9,999. Pinterest
The year 2020 witnessed the launch of several new smartwatches from various players, ranging from high-end wearables to the affordable Realme devices, in India.
Realme watch was the most shipped wearable watch device/model in both Q2 and Q3 in India, according to the IDC. Realme watch captured a 21.4 percent market share in Q2 and a 24.2 percent market share in Q3.
To gain a fresh start in the smartwatch segment in the new year, Realme has announced two new smartwatches — Watch S and S Pro — with a perfect mix of sport and style. This series marks the first time Realme has come up with smartwatches having round dials.
The Realme Watch S is priced at Rs 4,999 in India, while the Realme Watch S Pro's price is set at Rs 9,999. Let us see if Realme Watch S Pro justifies its arrival in a price segment dominated by the likes of Mi Watch Revolve, Huami Amazfit GTS, Honor Watch Magic, Huawei Watch GT and Fossil Sport Unisex, among others.
The smartwatch comes with a circular dial circular stainless-steel dial featuring analog markings around the bezel. In terms of dimensions, the Realme Watch S features a 47mm case, which is 12mm thick and weighs 48g.
The watch also comes with a variety of straps to change the look according to users' preferences and these straps are soft on the wrist and offer a comfortable wearing experience.
The smartwatch comes with a circular dial circular stainless-steel dial featuring analog markings around the bezel. Pinterest
The watch features dual physical buttons simply placed for convenient access on either hand.
The buttons are used to go back to the home from any screen. While the top crown also functions as a power on or off button, the bottom crown gives direct access to the workout modes.
The Watch S Pro also features a large 1.39-inch AMOLED touchscreen with 450 nits of brightness and 326 ppi.
It also gets an always-on display and an auto-brightness feature, one can also manually set brightness levels, but the 'auto display' feature works well.
The display also looked good in different light conditions and has access to a total of 108 watch faces, with the option to create your own watch face.
In addition, a 2.5D Corning Gorilla Glass layer prevents the AMOLED screen from scratches and damages.
The watch runs on a Realme's in-house wearable OS that offers several useful features. One would need the Realme Link app to enable all features and to view complete health data on a smartphone.
It allows the user to control music set a timer, check weather data, enable a flashlight, uses a stopwatch, set an alarm and more.
The device comes equipped with a 6-axis accelerometer sensor and a Heart rate sensor that provides 24-Hour heart rate measurement and Automated heart rate measurement.
The sensor also measures Resting heart rate, Exercise heart rate, offers Heart Rate Alert, and also takes Blood Oxygen measurement.
The smartwatch offers automatic sleep monitoring and can also track heart rate while you sleep. It can also track sleep duration as well as the different sleep stages, including deep, light and REM.
There are 16 sports modes in total – Outdoor Run, Indoor Run, Walk, Outdoor Cycle, Strength Training, Football, Basketball, Yoga, Cricket, Aerobic Capacity, Badminton, Indoor Cycle, Stationary Bike, Elliptical, Rowing Machine and Table Tennis.
The Watch S pro offers a 5ATM water-resistance rating, which makes it water-resistant for swimming sessions.
To gain a fresh start in the smartwatch segment in the new year, Realme has announced two new smartwatches — Watch S and S Pro — with a perfect mix of sport and style. Pinterest
It comes with a 420mAh battery which claims to offer up to 14 days of battery life. It also comes with a magnetic charging case that charges the watch to 100 per cent in just 2 hours.
Conclusion: At under Rs 10,000 category, the Realme Watch S Pro is the best budget smartwatch to buy in 2021. It looks highly similar to the Mi Watch Revolve and will definitely challenge it in the fast-growing wearable market.
ALSO READ: Here's Why Battery Cells Used in Vapes, E-Cigarettes, Flashlights and Toys Can Kill You
As health becomes the topmost priority for millions in the social distancing times, wearing a health buddy 24/7 makes perfect sense to go for Realme Watch S Pro. (IANS)
Scientists Develop Smartwatch App for Deaf People
Smartwatches offer people a private method for getting notifications about their surroundings. Unsplash
Scientists, including one of Indian-origin, have developed 'SoundWatch', a smartwatch app for the deaf and hard-of-hearing people who want to be aware of nearby sounds.
Smartwatches offer people a private method for getting notifications about their surroundings — such as a phone call, health alerts, or an upcoming package delivery.
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When the smartwatch picks up a sound the user is interested in — examples include a siren, a microwave beeping, or a bird chirping — SoundWatch will identify it and send the user a friendly buzz along with information about the sound.
"This technology provides people with a way to experience sounds that require an action — such as getting food from the microwave when it beeps," said study author Dhruv Jain from the University of Washington in the US.
"But these devices can also enhance people's experiences and help them feel more connected to the world," Jain added.
"I use the watch prototype to notice birds chirping and waterfall sounds when I am hiking. It makes me feel present in nature. My hope is that other deaf and hard-of-hearing people who are interested in sounds will also find SoundWatch helpful," he added.
The study was presented on Wednesday at the ACM conference on computing and accessibility. Unsplash
The team started this project by designing a system for deaf and hard-of-hearing people who wanted to be able to know what was going on around their homes.
The first system, called HomeSound, uses Microsoft Surface tablets scattered throughout the home which act as a network of interconnected displays.
Each display provides a basic floor plan of the house and alerts a user to a sound and its source.
The displays also show the sound's waveforms, to help users identify the sound, and store a history of all the sounds a user might have missed when they were not home.
The researchers tested HomeSound in the Seattle-area homes of six deaf or hard-of-hearing participants for three weeks.
Participants were instructed to go about their lives as normal and complete weekly surveys. Based on feedback, a second prototype used machine learning to classify sounds in real-time.
The first system, called HomeSound, uses Microsoft Surface tablets scattered throughout the home which act as a network of interconnected displays. Unsplash
The researchers created a dataset of over 31 hours of 19 common home-related sounds — such as a dog bark or a cat meow, a baby crying and a door knock.
The researchers then pivoted to a smartwatch system, which allows users to get sound alerts wherever they are, even in places they might not have their phones, such as at the gym.
Want to read more in Hindi? Checkout: परदे पर सभी महिला एवेंजर्स आएँगी एक साथ
Because smartwatches have limited storage and processing abilities, the team needed a system that didn't eat the watch's battery and was also fast and accurate.
First, the researchers compared a compressed version of the HomeSound classifier against three other available sound classifiers.
Also Read: 7 In 10 Urban Indians Are Hooked In Playing Mobile Games
Then, the researchers tested the SoundWatch app and found the app was useful for letting them know if there was something that they should pay attention to.
The study was presented on Wednesday at the ACM conference on computing and accessibility. (IANS)


text:
Small Portions + the Economics of Eating
By Anne Miller
SourceShutterstock
Good food and drink will forever remain one of life's greatest pleasures. Here's how to make it even better.
If you've got the dough to drop on a fancy meal, here's a tip: Don't ask for seconds. So goes the Emily Post-style etiquette set out by Thomas Keller, a colossus among chefs, who presides over some of America's most sought-after restaurant tables. (Don't even consider New York's Per Se if you're not prepare to blow about $350 per person, minus the wine.)
But don't you dare ask for seconds, even for the tiniest plates. As Keller told the author of the book Smart Chefs Stay Slim:
"Our whole menu is based on the law of diminishing returns. The most compelling portion of a dish is in the first three or four bites. With the first bite, you're getting into it; by the second bite, you start to realize it; and it is at the third or fourth bite you get the maximum appreciation and pleasure from that dish … and you keep eating because of that memory of it being really extraordinary. But was it as good [at the end] as it was at that second, third or fourth bite? No."
Keller was right. And now scientists — not just snobs — have proven it.
If you want to enjoy your wine or your meal more, take it slow and small. Forswear overindulging, and you'll take greater pleasure from what you're ingesting.
Restaurants that super-size their portions are actually harming their own business.
That's what Stanford University researchers found in a series of studies about feeling sated. In one, for example, more than 100 undergrads were given a handful of crackers of different flavors and asked to rank how much they liked the different flavors of cracker.
The smaller the portion, the more students reported liking that flavor. So the flavor of a cracker doled out 15 at a time was ranked worse by students than the flavor of cracker doled out just three at a time. Less was more when it came to what the students reported liking.
Emily Gabinsky, the study's lead author, even goes so far as to say that restaurants that super-size their portions are actually harming their own business, because people will fill up so much they won't be eager to return to buy seconds of the dish.
That's almost counterintuitive to practices in many popular, less shi-shi eateries — and even in our own homes: Think massive food-centric celebrations, whether a big fat Greek wedding or Thanksgiving dinner.
Bigger? Ain't always better.
Anne Miller, OZY Author Follow Anne Miller on Twitter Contact Anne Miller
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text:
a-, ab-, abs-
(Latin: prefix; from, away, away from)
This prefix is normally used with elements of Latin and French origins (abs- usually joins elements beginning with c, q, or t).
The form ab- is regularly used before all vowels and h; and it becomes a- before the consonants m, p, and v. The prefix apo- has similar meanings.
This list is a very small sample of the multitudes of a-, ab-, abs- prefixes that are available in dictionaries and those in this unit are only meant to present a few examples.
You can greatly expand your word knowledge in this
and in all of the other word units.
abstention (s) (noun), abstentions (pl)
1. A voluntary decision to do without something: Susan's abstention from chocolate was a choice she made because it was causing skin problems.
2. A refusal to ballot either for or against a proposal: The committee vote resulted in five ayes, ten nays, and four abstentions.
3. A deliberate rejection: The abstention by the mayor during the vote resulted in the proposal being defeated.
absterge (verb), absterges; absterged; absterging
1. To wipe or to make clean: Tom and Jerry were absterging the car before the family started on their trip.
2. To cleanse; hence, to purge: Rebecca, the nurse, absterged Manfred's wound with disinfectants and a special lotion.
abstinence (s) (noun), abstinences (pl)
1.The action or process of voluntarily refraining from some behavior or practice; self-control: While trying to lose weight, Steve's sister applied abstinence from excessive red meat and junk food, instead she ate a diet rich in fruits, vegetables, and whole grains all of which made her feel better and even look better.
2. The self-denial of something that is wanted or enjoyable: Jeremy started to drink again after a long period of total abstinence from alcohol consumption.
Abstinence may refer to a rejection of certain foods and drinks thought to be harmful to a person's health, however it can also refer to refraining from a behavior that is considered immoral.
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Go to this Word A Day Revisited Index
for a list of additional Mickey Bach illustrations.
abstinent (s) (noun), abstinents (pl)
A person who is self-disciplined and does not indulge in one's appetite for food or drink: As an abstinent, Greg, the coach, was described as a role model for the players on the football team.
abstinent (adjective); more abstinent, most abstinent
A reference to a person's character of sparing in the consumption of food or drink: The abstinent qualities of Audrey's aunt were much admired by her friends and relatives.
abstinently (adverb); more abstinently, most abstinently
A reference to how someone restrains his or her appetites or desires: Russell abstinently practiced self-denial so he could provide for his family's needs.
abstistic (adjective); more abstistic, most abstistic
1. A reference to all conversations, writings, and arguments that are based on theoretical principles, positions, political persuasions; pertaining to data that is devoid of the heart, the voice, poetry, or ideology rather than experience: The essay was an abstistic model of nothing but compassionless ideas and statistics.
2. Etymology: from abstract, meaning "disembodied, divorced from being perceived by the senses or the mind or being handled or touched or felt" + statistic, "facts that describe a situation".
abstract (s) (noun), abstracts (pl)
1. A theorisation that is not concrete and does not relate to real objects, but expressing something that can only be appreciated intellectually: The scientific abstract that was published was very academic and challenging.
2. A concept which is not easy to understand and is based on general principles or theories rather than on specific instances: The abstract about light absorption was understood by very few people and so it was read exclusively by a select group.
3. A brief statement of the essential thoughts of a book, article, speech, court record, etc.: The clerk will prepare an abstract for the judge to read.
4. A summary of a longer text, especially of an academic article: The students were instructed to write short abstracts outlining the main points in the article that was presented to them.
5. A concept or term that does not refer to a concrete object but denotes a quality, emotion, or idea, such as truth, passion, loathing, etc.: The aria from the opera emoted on the abstracts of love, hate, and revenge.
6. A presence existing only in the mind and separated from embodiment: Clarence referred to abstracts like "truth" and "justice".
7. A work of art, especially a painting, in a style that expresses the artist's ideas or feelings instead of showing the exact appearance of people or things and does not represent or imitate external reality or the objects of nature: The artist's style of painting could only be described as abstract; because it was so difficult to understand.
8. Etymology: the word abstract was formed from two Latin word parts, ab-, "off, away from" + tract, "to draw, to pull".
Abstract originally meant "drawn" or "taken from", such as part of a text taken or "abstracted" from a larger piece of writing. Then abstract came to mean "difficult to understand" or "pulled away from easy understanding".
Later in the late 1800s, the word's meaning changed to refer to a new artistic style, that is abstract art when it suggested that the artists' pulled away from reality and were more concerned with presenting forms and ideas than in representing actual people and things.
abstract (verb), abstracts; abstracted; abstracting
1. To take away; to remove without permission; to filch, to steal: While the thieves were in Dawson's house, they found and abstracted money, jewelry, and a computer from his residence.
2. To write a short summary of a speech, report, or other piece of writing: Einstein's theory of relativity is said to be abstracted from data gathered in several scientific experiments.
abstractedly (adverb); more abstractedly, most abstractedly
Pertaining to how something is said or done in an absentminded or preoccupied manner: Lorraine abstractedly answered the question as if her words were the beginning of a train of thought that were too fast to be expressed in normal speech.
abstractedness (s) (noun) (no plural)
A preoccupation with something to the exclusion of other things: Alan's abstractedness indicated a condition of deep absorption or thoughtfulness about other matters.
abstraction (s) (noun), abstractions (pl)
1. An idea or a way of thinking that is not related to real situations or practical experiences: The professor told his class that in some cases, "beauty" and "truth" were simply abstractions.
2. An emotional or mental condition that takes a person's attention away from what is happening around him or her: Mildred was looking out of the classroom window in abstraction whileD she was thinking about her sick mother during the teacher's presentation.
3. The act of obtaining or removing something from a source: Dr. Black told Sam, his patient, that the abstraction of the tumor during surgery went well and that it was not malignant!
abstractionism (s) (noun), abstractionisms (pl)
Creative or cultured content that depends more on internal form rather than pictorial likeness or illustration: The student artist was told that abstractionism was simply a representation that had no particular reference to concrete objects or specific examples.
abstractionist (s) (noun), abstractionists (pl)
1. An artist who paints or creates art that expresses ideas and emotions by using elements of colors and lines without attempting to create a realistic picture: Lawrence's sister was a well-known abstractionist who was very creative with her unique forms and the blending of colors in her artistic endeavors.
2. Those who do not represent or imitate external reality or the objects of nature: The abstractionists were criticized for not presenting realistic ideas for improving the parks, streets, and other aspects of the city that had been deteriorating for years.
abstractive (adjective); more abstractive, most abstractive
A descriptive term for anything that is concerned primarily with theories or hypotheses rather than practical considerations: There are too many abstractive definitions presented by dictionaries that are repetitious with words that include a slightly different format of the main entry and which result in definitions that are abstruse, that lack clarity, or are simply too difficult for readers to comprehend.
Showing page 11 out of 13 pages of 190 main-word entries or main-word-entry groups.


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You are here: Home » Newsmakers » News Release » Malouf Expands Sales Team
Malouf Expands Sales Team
Malouf Restructures Sales Team to Account for Growth
LOGAN, Utah—Malouf™ has evaluated new growth patterns and is restructuring their sales team to meet demand across North America. Eric Holmstead will take the lead as director of North American sales, expanding his role from national sales manager. To serve the new growth across the U.S., Malouf™ has reestablished a three-district model, with a strong central sales region led by new manager Brian Watts. The team has also added three additional territory managers: two current employees, Kristin Barker and Scott Richins, and new employee Jano Reese.
Jano Reese
Scott Richins
Kristen Barker
Brian Watts
Eric Holmstead
"We're trying to stay proactive with this restructure to ensure that our stellar customer service and consultation with our customers never wavers," said Eric Holmstead, North American sales director. "We realized that with large overall growth, especially in the Midwest, and big goals for the future, it was time to make some changes."
The Central District will include accounts west of the Mississippi River, stopping at the line of the Dakotas. Malouf™ has added hundreds of new accounts in the region this year, along with adding new categories to existing accounts. The rapid growth has led to restoring a full-time manager, which will also allow Jeff Holmstead and Jesse Gibbs to consolidate attention on the growing East and West Districts.
New Central District Manager Brian Watts has worked at Malouf™ for four years as the South Atlantic territory manager. Eric Holmstead said of Watts, "We're excited for Brian to work in this new role, and for the support he'll bring to the Central District. His work will not only relieve pressure on the other territories, but also bring a new perspective and exciting ideas, like his vision for merchandising." Holmstead finished, "Most importantly, Brian will lead his territory managers and outside sales reps in helping our retail partners to be as successful as possible."
To support growth across the country, Malouf™ has three new territory managers alongside the changes in Holmstead's and Watts's roles. Kristen Barker was previously an account manager in the Northeast. She will now manage the Midwest Central region, which includes Kansas and Missouri. Scott Richins, also a former account manager, will serve the Northeast region of Michigan, Indiana, Ohio, Kentucky and West Virginia. Jano Reese is new to the Malouf™ team and will act as the territory manager in South Atlantic area, which encompasses Virginia, North Carolina, South Carolina and Georgia. Reese is taking over for Brian Watts.
Another change to the team will come at the end of the year when Vice President of Sales Mike Douglas will be stepping down to join the executive team at Sleep Country/Dormez-vous. Douglas's move cements the growth partnership between Sleep Country and Malouf™ after their successful product launch earlier this year with the Canadian retailer.
The Malouf™ sales department consists of over 40 in-house team members and 60 outside field representatives that manage more than 6,000 retail partnerships across North America, South America, Asia, Europe and Australia. To partner with Malouf™ or learn about its products and capabilities, contact a sales representative at 800-517-7179 or [email protected]
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The vocabulary of music is vast with a unique lexicon for each genre. This quiz is focused on words within the western classical tradition. We'll be back with more music quizzes for other genres soon enough.


Setting aside the ceramics and sculptural works that have dominated his more recent output, for his first solo show at Zero …, entitled 'Il mio cuore sulla spiaggia' (My Heart on the Beach), Alessandro Pessoli returned to focus on canvas, using brushwork, screen printing and stencils. While there is no doubt about Pessoli's deep-rooted belief in the elastic possibilities of painting, here the choice of medium seemed a deliberate trip down memory lane, one possibly aimed at renegotiating the formal and theoretical framework of the artist's production to date (and, given that his oeuvre spans three decades, hopefully one made in anticipation of a much-deserved retrospective of his work).
In this new series of paintings, comprising 14 (mostly small) canvases, one recurring theme was still lifes of fish. At first sight, this subject matter may appear less psychologically and emotionally charged than others previously chosen by Pessoli – such as his visionary rendezvous with religious imagery, or his investigations of how personal histories are interwoven with the larger, national narrative. Nonetheless, of all the possible subjects for a classical still life composition, fish are perhaps the most puzzling (could it be because they evoke both religious and sexual references at the same time?) and possibly also the most melancholic (perhaps because of their eyes, always wide open and staring sadly at the onlooker). This sense of melancholia was reinforced by other motifs in the paintings on display – empty boats, tiny anchors, deserted seashores – and by the subtle resemblance to the kind of fine art reproductions one might find in a pizzeria by the sea: faded mechanical copies of Canaletto and the like. A rather comforting feeling of nostalgia was also suggested by the cartoonish approach with which the subjects are often treated, evoking Bruno Bozzetto's animated cartoon series from the 1970s, an iconography that Pessoli has frequently explored.
The artist's nimbleness of thought, enhanced by his colourful imagination, allows for such non-stop shifts between themes, motifs and genres. In fact, it has always been standard procedure for Pessoli to mix personal associations with references to popular culture and allusions to art history, from the Italian Renaissance to European pop – or, in this case, the long tradition of marine-themed still life in the work of 20th-century Italian painters such as Carlo Carrà, Filippo De Pisis and Scipione. Nonetheless, the almost complete absence of the human figure, which has been prominent throughout Pessoli's career up to this point, is certainly atypical. There are no bodies, only the occasional face – the artist's own – emerging from a pile of fish, hiding behind stylized sunglasses or peeping out of a sailing boat. Rather than being the subject of his paintings, though, Pessoli appears as a bystander, caught while yearningly observing the same scene that we are viewing, presumably of the waterfront of Cervia, the seaside resort on the Adriatic Riviera where the artist was born and brought up.


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John Bulmer (1784 - 1857) was an Independent minister. He was born and grew up in Yorkshire, England, and was  educated for the Congregational Ministry at Rotherham (Masborough) Independent College. He moved to Wales as pastor of Albany Meeting House, Haverfordwest in 1813. Whilst there he worked on and published a number of volumes of verse, sermons, and other religious material.

In 1840 he left Haverfordwest for a position in Rugeley. He held further ministerial positions in Bristol, Newbury, and (after an interval) at Langmore and Ruxton near Ross.

He died in 1857.

References

Welsh Protestant religious leaders
1784 births
1857 deaths
19th-century Welsh clergy
19th-century English clergy


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1. Praeludium
2. Open Masterclass
3. Amitie
4. Ensemble Renard
5. Tzigane
6. Family Concert
7. Danse Rustique
8. Tango del Diablo
9. Sergio Bucheli
10. Festival Finale
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Emma Roijackers
Emma has established herself as an active chamber musician, soloist and orchestral player, performing in the best halls of The Netherlands including The Concertgebouw (broadcast live on national radio) and all over Europe. Emma regularly collaborates with some of Holland's most famous musicians including Maria Milstein, Caspar Vos, Lisa Jacobs, Michael Zemtsov. She has also been invited to play at several renowned international chamber music festivals, such as the International Chamber Music Festival in Utrecht in Holland, the Francigena International Arts Festival and the Festival Internazionale di Musica (Portogruaro) in Italy, and the Festival Internacional De Música De Marvão in Portugal.
During the 2021-2022 season Emma will be giving a variety of chamber music concerts, recitals concerts and concertmaster appearances including concerts in Germany, Hungary, Corsica, Portugal and Switzerland and Emma will be returning to the major halls in The Netherlands. She will also appear as a soloists in works by Beethoven and Bruch. She has been given full financial support to create her very own education concert series, which she will develop and perform in schools all over the Netherlands this season.
Emma has received a number of awards including the 'Laberté Hoedemaker at the Peter de Groote Festival and she received numerous prizes at the National Prinses Chrsitina Competition. She has also been accepted as one of the twelve participants to Crans- Montana Classics in Switzerland, with artistic director renowned violinist Schlomo Mintz. Recent concerto appearances have included performances of works by Brahms, Bach, Mozart and Vasks.
Emma was concertmaster of the ensemble 'The String Soloists' where she has also appeared as a soloist. Their most recent CD was praised by BBC Music Magazine as being 'Crisp clean playing' and by Musicweb International 'Style and sensitivity meets the exquisite and sophisticated'. Since this year She plays with the Cologne chamber orchestra as second concertmaster. Emma is regularly asked to play with the Amsterdam Sinfonietta, the Residence Orchestra The Hague, The Noord Nederlands Orchestra and the Philharmonic Orchestra of the Netherlands.
Emma's studies began at the junior department at the Conservatory of Amsterdam. Emma continued her studies at the Conservatory of Amsterdam under Prof. Ilya Grubert and graduated with the highest mark "10". She then received full funding from the Netherlands to continue her studies with Lukas Hagen in Salzburg where she is currently finishing her post-graduate diploma. Additional inspiration has come from lessons and masterclasses by world famous violinists and musicians such as Christoph Poppen, Leonidas Kavakos, Gerhard Schultz, Schlomo Mintz, Schmuel Ashkenasi, Zakhar Bron, Ray Chen and Jaap van Zweden.
Emma plays on a violin built by Giovanni Grancino (1700-1705, Milan), generously given to her on extended loan by an anonymous benefactor.
www.emmaroijackers.com
With grateful thanks to our generous sponsors without whom this Festival would not be viable:
©2023 by Romsey Chamber Music Festival, Registered Charity No. 1182043


When I type FS (short for Forest Service) MMS changes it to SF. I don't know why.
I have added FS to the User Dictionary but the switch to SF still occurs. I'd like to find where the auto correct list is to delete SF but I can't find it.
Is there a way to edit the auto correct list that I've overlooked?
Click on Tools > Edit User Dictionary.
Open the language file you are using, most likely User_us.lxa.
Add the word FS to the dictionary by itself as opposed to FS:FS.
This doesn't appear to be an issue on Macintosh.
Click on Spell > Edit User Dictionary.
I'm on a MAC -- Tools -- Edit User Dictionary -- choose USERDICT.LXA -- make changes and nothing happens. Still auto corrects to incorrect. I can't find where I could edit the file outside of MMS.
I suppose I've developed a glitch. MMS has been on my machine for a REALLY LONG time.
It's a very annoying glitch.
cmw7 wrote: Thank you for your answer.
This is a known issue that prevents the dictionary from being edited while a file is open. Try closing all documents, then going to Tools > Edit User Dictionary and adding the word.
We have an update in the works that is almost ready for beta testing. This issue will be completely gone when that is issued.


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Tag Archives: David Crosby
Waging Heavy Peace (an excerpt)
Click here to read an excerpt from Waging Heavy Peace: A Hippie Dream by Neil Young:
Click to access NeilYoungWagingHeavyPeaceExcerpt.pdf
Tagged as 1953 Buick Skylark, 1970, A Hippie Dream, A Memoir, Amber Young, American Dream, an excerpt, Ben Young, blue rider press, book excerpt, book review site wordpress, Buffalo Springfield, country-rock music, Crosby, CSN, Dallas Taylor, David Crosby, Graham Nash, hardbound book release, Joseph's Reviews, Kindle Edition, Lionel Trains, memoirs, Neil Young, Nook Book, rock music, Stephen Stills, Stills & Nash, the Byrds, The Hollies, unabridged audiobook, Waging Heavy Peace, Waging Heavy Peace (an excerpt), WMG, Wordpress book review site
June 17, 2011 · 1:46 pm
It Don't Come Easy
Fire and Rain: The Beatles, Simon & Garfunkel, James Taylor, CSNY and the Lost Story of 1970 by David Browne (Da Capo, $26.00, 368 pages)
"Half the people are stoned/And the other half are waiting for the next election." Paul Simon
The year 1970, as some of us remember, was the year that Simon and Garfunkel's Bridge Over Troubled Water was both the best-selling album and single of the year. But what might not be remembered is that S&G would soon be targeted – during the very same year – as rock's ultra-conservative sell-outs. The New Yorker music critic, Ellen Willis, wrote of Mr. Simon: "I consider his soft sound a copout. And I hate most of his lyrics; his alienation, like the word itself, is an old-fashioned, sentimental, West-Side-liberal bore."
Not to be outdone, critic Miles Kingston (who claimed to be a fan) wrote: "Some people hate Simon and Garfunkel because their music has no guts, because it is a middle-class look at life, because it slips too easily from idiom to idiom." Kingston described their fans as "the left-out kids – the loners, the book-worms… (and worse)." And then there was the Time Magazine reporter, assigned to do a cover story on James Taylor, who wrote that, "…the people interested in James Taylor are those who never quite got over a fascination with Simon and Garfunkel. Upon whom it is now fashionable to dump."
Yes, David Browne has a knack for finding interesting bits and bytes of information that challenge our collective memory. This is a non-fiction account of the 1970s – and, specifically, the decade's beginning – in post Kent State America. Browne writes about the softening of rock 'n roll in a year that saw the demise of three of the world's most successful groups – The Beatles, Simon and Garfunkel, and Crosby, Stills, Nash and Young (CSNY). Yet, in a year that one publication initially termed The Year That Melody in Popular Music Had Died, it was to be a year of rebirth in music, of melody.
If the hard rock of the late 60s had just about killed melody (John Lennon had called Beatle Paul's Helter Skelter, "just noise…"), it was soon brought back to life in the form of new performers like James Taylor and Elton John. Browne's account is actually a melding of two – one, a background look at the music of the time; second, a description of the social and political environments of the late 60s/early 70s. In this it bears many similarities to Girls Like Us, an earlier-written account of the musical careers and times of Joni Mitchell, Carole King and Carly Simon.
I noted that Browne has a knack for finding interesting factoids. Here's another one… According to his research, backed by Paul Simon and Paul McCartney, two of the major songs of the decade were written not for the composer's own group/band but for the voice of Aretha Franklin. Yes, both Bridge Over Troubled Water and Let It Be were specifically written for the Queen of Soul, who – luckily for fate – rejected them. It's one reason that both songs, written within weeks of each other, share a gospel soul and structure.
If you'd like to read more fascinating things that you never knew about all of the band members and performers listed in the book's subtitle, and about others like Joni Mitchell, Jimi Hendrix, Bob Dylan, Phil Spector, Allen Klein, Mary Balin, and Billy Preston, you'll want to run and pick this one up. As James Taylor was to sing, "Hey, Mister, That's Me Up on the Jukebox!"
Notes – Girls Like Us: Carole King, Joni Mitchell, Carly Simon and the Journey of a Generation by Sheila Weller was reviewed on this site on February 2, 2011 ("Women of Heart and Mind").
Elizabeth Taylor was to say that, "People don't like sustained success." Which is perhaps why, in 1970, George Harrison sold more records than either Paul McCartney or John Lennon.
Tagged as 1970, 4 Way Street, All Things Must Pass, Allen Klein, Altamont, Aretha Franklin, Beatles break-up, Billy Preston, Bob Dylan, book review, Bridge Over Troubled Waters, Carly Simon, Carole King, CSN&Y, Da Capo, David Browne, David Crosby, Deja Vu, Dream Brother, Elizabeth Taylor, Ellen Willis, Elton John, Fire and Rain, George Harrison, Girls Like Us, Goodbye 20th Century, Graham Nash, Helter Skelter, Hey, If I Could Only Remember My Name, It Don't Come Easy, James Taylor, Jimi Hendrix, John Lennon, Joni Mtchell, Joseph Arellano, Joseph's Reviews, Kent State University, Kindle Edition, Let It Be, Los Angeles, Marty Balin, McCartney, melody, Miles Kingston, Mister, music critics, Neil Young, New Yorker, noise, Nook Book, Ohio, Our House, Paul McCartney, Paul Simon, Phil Spector, Plastic Ono Band, recommended books, Richard Nixon, Ringo Starr, rock music, Sea of Madness, Sheila Weller, Simon and Garfunkel, Songs for Beginners, Stephen Stills, Stephen Stills 2, That's Me Up on the Jukebox, The Beatles, the Lost Story of 1970, the Queen of Soul, The Troubador, Time Magazine, Topanga Canyon, Vietnam War protests, Women of Heart and Mind


This manual outlines the policies and procedures that are critical for the Van Buren County Drug Treatment Court program.
This is the participants handbook for the Gunnison Adolescent Drug Court program.
This is a form used to identify any alcohol related incidents involving DWI Court participants.
This document is an overview handbook for the Koochiching County DUI/Substance Abuse Court.
This document is the track orientation form that provides an outline of the conditions for the 4th phase of the Palm Beach County Drug Court program.


Bim Willow is coming back to the Arboretum in a tricked-out bus full of willow and you will not want to miss it! Bent willow furniture was an early American craft that is making a strong comeback. Bim is an experienced artist in this medium, and will walk you step-by-step through the willow project of your choice, resulting in a beautiful bent willow piece within a matter of a few hours--really! You will never feel more proud of the furniture building skills you didn't know you had. Prices listed are per project, and include a box lunch or dinner.
Projects are grouped by similar construction techniques; you will be working simultaneously alongside other participants regardless of which project you choose. Bim and his assistants will rotate through all participants to provide one-on-one attention as you complete each step. The amount of time to complete a project varies based on the student; the times listed are an estimate and students may leave whenever their project is completed.
These projects are best when assembled as a solo builder. Couples working on one project together will likely have one partner waiting/observing for long periods (which often leads to micromanaging your partner...nobody loves that!). We recommend couples work on their own solo projects simultaneously. Besides, what's better than than one chair? A set of chairs! See coupon offer below.
All registrations must be received no later than Friday, July 26 at 3pm in order for Bim to harvest the required willow materials for your project.
Register for 2 or more Willow Projects (either a couple working on 2 simultaneous projects OR a single completing both morning and afternoon sessions) and receive 10% off your total order!
Use coupon code TenPercent at checkout or call 612-301-1210.
Classes must be purchased at the same time in the same order, not valid on previous purchases or with other discounts. No cash value.
YES! A Birdhouse is at the top of the bench!
Approx Dimensions: 48" x 40" x 14"
Approx Dimensions: 46" 32" 28"


NeNe Leakes Lands In The Wall Street Journal!
It's easy to blame "govament" but we need to take stock of ourselves first. The epidemic of mediocrity that is sweeping this country is the biggest threat to our economic well-being. Do you see what is happening, folks? Underperformance is the new normal.
'True Blood' season finale: What was the 'bonus scene'? Find out here.
'Expendables 2' packs a punch. Viacom packs in more commercials.
KSL-TV, NBC's Mormon-owned Utah affiliate, will not air Ryan Murphy's new gay family sitcom TheNew Normal, citing rude and crude dialogue and deeming the.
When we last checked in with small but brave groups taking a stand against NBC's upcoming fall family-destroying sitcom The New Normal, the organization One.
"This program simply feels inappropriate on several dimensions."
The Salt Lake City, Utah-based television station KSL, an major NBC affiliate owned by the Mormon Church, has announced that it will not air the Peacock Network's upcoming comedy series The New Normal due to its "inappropriate content."
I suppose, the cliche "new normal" probably would be more appropriate. You know it as well as I do that Katrina changed us. And if you don't believe me, watch how we're acting now that a hurricane entered the Gulf of Mexico. Enter Isaac.
Joining us from newport beach . . . mohamed el erian, pimco's ceo and co-cio. Mohamed . . . let's talk about paul ryan . . . seems to be taking your phrase and using it. Is he using it properly? I saw him say we're here in the new normal. . . . we view it much more as a policy response that has been too cyclical, too partial, and too short term. So that's the key thing, andrew, i think, is to focus on what he said at the end of that speech. he said if we're not careful, for the first time in a very long time, our children's generation will be worse off than ours and that is a key issue that hopefully the politicians will focus on. . . . but the signal to the politicians is . . . you've got to act because our problems are getting embedded in the structure of the economy. . . .