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vice.com Go back Open original
FBI Shows Arrest Video Of Dark Web Kingpin Who Died By Suicide in Police Custody
In July 2017, the FBI shut down AlphaBay, one of the largest illegal dark web marketplaces in the world, and arrested its administrator, Alexandre Cazes. He died while in custody of Thai police days later by suspected suicide.
At a cybersecurity conference in Manhattan Tuesday, an FBI agent involved in the case showed a video of Cazes's arrest to journalists and law enforcement, and joked about how the arrest went down.
"See if you can spot the moment when he realizes he's about to be arrested," FBI special agent Nicholas Phirippidis said as he played a few seconds of the surveillance footage of the arrest at Fordham University's International Conference on Cyber Security; the audience laughed as the video played.
Cazes died eight days after the arrest. He was 26, and never got a trial.
Read more: These Are the Two Forgotten Architects of Silk Road
Phirippidis told the audience that the bureau managed to corner Cazes and arrest him while he was still logged in as the admin of AlphaBay by ramming a car through the front gate of his home in Thailand.
This footage has yet to be publicly released and the FBI declined to send us the actual video file, but as I realized what the video was, I filmed some of it on my phone. You can see a brief segment of the video and Phirippidis's comments below. The FBI declined to send us any of the materials used in the briefing and would not make an agent available for an interview.
On the right, you can see Cazes' house, and in the middle, Thai agents capture him after luring him out.
It is not unusual for the FBI to give talks at security conferences about closed investigations, but in this case, the FBI is promoting the arrest of a man who wasn't given a trial because he died while in the custody of law enforcement.
The FBI's plan was to crash the undercover police car onto Cazes' front gate and get him to come out. This wasn't just to get their hands on him, but also to get his computer before he managed to encrypt his data, something FBI directors have been warning against for years.
The idea was to get Cazes' computer unlocked and unencrypted to avoid having to potentially crack into it by breaking its encryption, Phirippidis said. Increasingly, police and federal agents try to seize computers while they are on and unlocked to avoid the Apple vs, FBI scenario, where feds initially couldn't access data on an encrypted iPhone.
Got a tip? You can contact this reporter securely on Signal at +1 917 257 1382, OTR chat at lorenzo@jabber.ccc.de, or email lorenzo@motherboard.tv
After crashing the car at the front gate, the agents played dumb, acting like they screwed up a three-point turn. After a minute that "seemed like an eternity," Phirippidis said, Cazes came downstairs, where the police took his phone and arrested him.
"When we get to his bedroom we see that he's actually logged in as admin on AlphaBay, which you're not going to get much better evidence than that," Phirippidis said, showing a screenshot of Cazes's laptop. "We got lucky."
The arrest was the dramatic end to an investigation that started two years earlier thanks to a Hotmail account that linked Cazes to his online, criminal persona on AlphaBay. With that first piece of evidence, Phirippidis and his colleagues at the bureau then "followed the money," or in this case, his Bitcoin transactions, payments, and cashouts—something that can be done in part with open source tools.
Phirippides closed his talk on another light-hearted note, explaining that the operation to get Cazes was called "Bayonet," because they wanted to make a "triple-pun" using the word bay, the word net for internet, and for catching "bad guys."
"It sounded like something," he said. "We Googled it and there wasn't one already, so we were 'alright!'"
The National Suicide Prevention Hotline is toll-free in the US and available 24/7 at 1-800-273-8255, while suicide.org has a list of international suicide hotlines , including Canada and the UK.


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Siah Armajani, Ceaselessly Imaginative Artist with a Belief in the Power of Public Art, Is Dead at 81
He had a 2018 retrospective at the Walker Art Center in Minneapolis.
Aug 28, 2020 1:13 pm
Minneapolis-based artist Siah Armajani's first retrospective in the United States, "Follow This Line," takes its title from a childhood memory: in prerevolutionary Iran, Armajani and his friends…
Michael McCanne
9 Art Events in New York This Week: Siah Armajani, Sarah Charlesworth, Cruising Pavilion, and More
A guide to the next seven days.
Armory Show Names Artist List for 2019 Curated 'Platform' Section
Organized by Sally Tallant, the presentation will include large-scale works by Siah Armajani, Tania Candiani, Xaviera Simmons, and others.
Claire Selvin
Siah Armajani at Walker Art Center, Minneapolis
See images from one notable show every weekday.
Fall Preview: The Most Promising Museum Shows and Biennials Around the World
Behold, 41 exhibitions to see—or at least read about—this season.
Sep 5, 2018 10:39 am
Have At It: A Post-Trump New York Looks at How Images, Ideas, and Resources Circulate
On MoMA's collection rehang, CAPTC, Mary Beth Edelson, Vikky Alexander, Lynn Hershman Leeson, Graham Anderson, Jason Loebs, Raymond Pettibon, A. K. Burns, and more
Liz Hirsch
Siah Armajani at the Kemper Museum of Contemporary Art, Kansas City, Missouri
Pictures at an Exhibition presents images of one notable show every weekday

redpajama_set_name: RedPajamaCommonCrawl


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Home » Norton Education Series » 2021 NES IFFGD 30th Anniversary Event
Current and Novel Treatment Options for Pelvic Floor Disorders
Presented by Satish Rao, MD, PhD, FRCP, FACG, AGAF
Dr. Satish Rao
Click here to watch presentation
Presentation Overview:
Anorectal and Pelvic floor disorders affect 25% of the population and cause significant distress including effects in quality of life. This includes many conditions such as dyssynergic defecation, and fecal incontinence, and rarely descending perineum syndrome, rectal mucosal intussusception/prolapse, solitary rectal ulcer syndrome and levator ani syndrome. First step is to take a detailed history. Because of the irregularity of bowel function, new digital Phone APPs such as Constipation Stool diary, My GI App and Fecal Incontinence stool diary can be very useful tools for patients and clinicians for accurate record and monitoring of bowel symptoms. Diagnosis of these problems remains largely clinical supported by radiology and/or manometric tools. Today there are excellent novel tools for diagnostic evaluation, including high resolution anorectal manometry, high definition 3-D anorectal manometry that uses TachArray and 256 pressure sensor probe. Additional tools including translumbosacral anorectal magnetic test for neurophysiology and balloon expulsion and colon transit studies.
Randomized Control Trials (RCTs) have shown that biofeedback therapy is effective for treatment of dyssynergic defecation, and a recent study showed that home biofeedback therapy is as effective as office biofeedback therapy and is cost-effective. In a recent RCT, both syringe and barostat assisted sensory training were useful in treating rectal hyposensitivity associated with dyssynergic defecation, but barostat training was more convenient and superior. Although biofeedback therapy remains the mainstay for treatment of fecal incontinence, newer treatments such as Dextranomer beads when injected into the anal region has been shown to be more efficacious than placebo. Sacral nerve stimulation has also been shown to be useful in fecal incontinence and home biofeedback with a new device has been shown to be as useful as office biofeedback. Most recently a novel treatment using repetitive magnetic stimulation holds promise and in a randomized controlled trail, translumbosacral neuromodulation therapy was shown to be effective and safe and improve both neuropathy and symptoms.
In conclusion, there is a new ray of hope for patients with these disorders as new tools for diagnosis are evolving, and efforts are being made to better characterize symptoms and phenotypes, and novel approaches of treatment are being developed some of whom are undergoing RCTs.
About Dr. Rao:
Professor Satish Rao is the J. Harold Harrison, MD, Distinguished University Chair in Gastroenterology and Founding Director of the Digestive Health Center and Digestive Health Clinical Research Center and Director, Neurogastroenterology/Motility and tenured Professor of Medicine at Augusta University, USA. He served as the Chief, Division of Gastroenterology/Hepatology, Director, GI Service line and GI Fellowship Program Director. Previously, he was Professor of Medicine & Director, Neurogastroenterology/motility at University of Iowa Carver College of Medicine, Iowa.
Satish Rao received his MBBS from Osmania Medical College, Hyderabad, India, his PhD from the University of Sheffield, UK, and Fellowship of the Royal College of Physicians (FRCP), UK. He completed his internal medicine training at several centers and a clinical research fellowship at the University of Liverpool, UK. He is American Board certified in Medicine and Gastroenterology. He is past President, American Neurogastroenterology/Motility Society.
His research interests in the field of Neurogastroenterology/Motility have focused on gaining mechanistic insights, developing novel diagnostic tools and pioneering innovative treatments for common disorders especially constipation, dyssynergic defecation, fecal incontinence, IBS, food intolerance, gas and bloating and small intestinal bacterial and fungal overgrowth (SIBO/SIFO) and visceral pain. He is credited with discovering dyssynergic defecation, a problem that affects 1/3rd of constipated patients and its remedy, biofeedback therapy as well as novel treatments, sensory adaptation training for rectal hypersensitivity. He has pioneered several novel tests, translumbosacral anorectal magnetic stimulation for pelvic floor neuropathy, esophageal balloon distension for chest pain, fructose and fructan breath tests. He developed the first method for examining bi-directional gut and brain interactions in humans. His latest invention translumbosacral neuromodulation therapy (TNT) is revolutionizing treatment for fecal incontinence. He has been awarded 4 patents.
Dr. Rao is a National Institutes of Health funded principal investigator for 20 years and currently holds $ 10.6 million NIH UO-1 and 3.8 million RO-1 grants. Dr. Rao has edited 10 books, including Handbook of GI Motility (2015), and Clinical and Basic Neurogastroenterology & Motility (2020). He has published over 500 peer reviewed articles with 22,300 citations, a H index of 87 and i10 index of 235. An astute clinician, Dr. Rao has been selected as one of the "Best Doctors in America" and as Americas' Top Doctors for over 25 years. He has mentored over 100 Physicians from across the world, many of whom are leaders and professors in their own countries. Thirty of his mentees have received national/ international research awards- a testament of his dedication as a distinguished mentor. He has been invited to lecture in 35 countries as visiting professor. These include live demonstrations of motility procedures and novel TNT procedures, and to train gastroenterologists and fellows globally and at multiple institutes in India and all 5 continents.
Dr. Satish Rao has received the 3 highest meritorious honors from the American Gastroenterological Association (AGA), the AGA Distinguished Clinician Award, AGA Masters Award for Outstanding Clinical Research and AGA Distinguished Educator Award. He received the American College of Gastroenterology Auxiliary Research Award, the International Foundation for Functional GI Disorders Senior Clinical Investigator Award, University of Iowa Regents Distinguished Award for Faculty Excellence, "Dr. PN Chuttani Oration", the highest honor from Indian Society of Gastroenterology, and the Augusta University Distinguished Research Award and the Medical College of Georgia Distinguished Faculty Award and Mexican Society Gastroenterology Honorary Diplomate/Fellow. Dr. Rao is one of a rare breed of academicians referred to as a tripled threat, and has excelled as an internationally renowned researcher, distinguished educator and outstanding physician.
Topics of this article
Introduction: Overview of the Gastrointestinal (GI) System by Shanti Eswaran, MD
Disorders of the Throat and Esophagus by John Pandolfino, MD
Disorders of the Stomach by Jan Tack, MD, PhD
Disorders of the Small Bowel by Lucinda Harris, MD
Disorders of the Colon and Rectum – Dr. Baharak Moshiree
Physician Introduction on Short Bowel Syndrome (SBS) and Intestinal Failure Adam Stein, MD
Surgeon Introduction on Short Bowel Syndrome (SBS) and Intestinal Failure by David Mercer, MD, PhD, FRCSC, FACS
Patient Perspective on Short Bowel Syndrome (SBS) by Swapna Kakani
Patient Perspective on Intestinal Failure John Mahalchak
Physician Introduction on Irritable Bowel Syndrome (IBS) Lin Chang, MD
Patient Perspective Irritable Bowel Syndrome with Constipation (IBS-C) – Deb Caton
Patient Perspective on Irritable Bowel Syndrome with Diarrhea (IBS-D) – Lisa Strykowski
History of FGIMDs and IFFGD with Dr. Drossman – Dr. Douglas Drossman
Physician Introduction on Small Intestinal Bacterial Overgrowth (SIBO) Darren Brenner, MD
Physician Introduction on Helicobacter Pylori (H. Pylori) Presented by William Chey, MD
Patient Perspective on Small Intestinal Bacterial Overgrowth (SIBO) Tina Aswani Omprakash
Pharmacologic and Surgical Treatment for Esophageal Disorders by Ronnie Fass, MD
Pharmacologic and Surgical Treatment for Disorders of the Stomach Michael Camilleri, MD
Pharmacologic and Surgical Treatment for Small and Large Intestine, Including Bloating – Dr. Brian Lacy
Current and Novel Treatment Options for Pelvic Floor Disorders – Dr. Satish Rao
Behavioral Treatments for Gastrointestinal Disorders – Laurie Keefer, PhD
Dietary Interventions for Gastrointestinal Disorders by Emily Haller, MS, RDN
Complementary and Alternative Medicine (CAM) for Gastrointestinal Disorders by Anthony Lembo, MD
Physician Introduction on Cyclic Vomiting Syndrome-Cannabis Hyperemesis Syndrome by Thangam Venkatesan, MD
Physician Introduction on Functional Dyspepsia-Gastroparesis Braden Kuo, MD
Patient Perspective on Cyclic Vomiting Syndrome (CVS) James O'Reilly
Patient Perspective on Gastroparesis – Trisha Bundy
Physician Introduction on Constipation Anthony Lembo, MD
Physician Introduction on Diarrhea – Dr. Brooks Cash
Patient Perspective on Constipation – Erin Slater
Physician Introduction on Heartburn and Reflux by John Pandolfino, MD
Physician Introduction on Eosinophilic Esophagitis (EoE) Evan Dellon, MD, MPH
Patient Perspective on Gastroesophageal Reflux Disease (GERD) Luiz T. Yamashita Jr.
IFFGD is a nonprofit education and research organization. Our mission is to inform, assist, and support people affected by gastrointestinal disorders.
Our original content is authored specifically for IFFGD readers, in response to your questions and concerns.
If you found this article helpful, please consider supporting IFFGD with a small tax-deductible donation.


At Dumpster Centre, we strive to bring you the best service at the best price available. We have a wide selection of dumpster sizes in stock in Callands, VA, with flexible & timley pickup and delivery. Give us a call today for all your dumpster rental and roll off needs.
Plastic bottles, disposable discs, glasses and also other sort of big and small garbage gets heaped after you clean up a garage or perhaps store bedroom. Here is a one stop solution for all you garbage relevant problems.
You could possibly did not much like the master bedroom after you moved in your home. You and your spouse bought the property knowing that you should remodel the main bedroom. The time has come to get the remodeling occupation taken care of.
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we're in major sale mode at the shop!
loads of xmas and non xmas merch marked to 50% off.
definitely worth perusing if you find yourself downtown.
we will be open all weekend with normal hours but will be closing monday, new years eve at 4. closed new years day, reopening wednesday january 2nd.


Here are more than 10 free sewing patterns for your kitchen - cute, useful and free sewing projects for you to start and complete really fast. They include free sewing patterns and will give your home a fresh feel of a makeover while only requiring a little fabric. These sewing ideas for kitchen make super handy DIY gifts for friends, for housewarming parties, and for your own home decoration.
No back to the easy and free patterns for the kitchen! Follow a few of these simple tutorials and grab a few free patterns to give your kitchen a makeover on a budget. You'll see that a lot of these sewing ideas are inexpensive scrap busters (napkins, patchwork, coasters), but very handy for your household (think your own handmade kitchen potholders, grocery bag holders, aprons!) Some of them are so cute I could simply not resist them - and trust me, you won't be able to, either!
Make your own customized fabric covers with this easy and quick bowl cover tutorial. So cute!
These easy felt coasters will be an eye-catcher at any party, and make a perfect homemade present! A tutorial with step-by-step directions at THIS link.
If you've ever wanted to make a mini quilt, this cute little stylish potholder is an excellent chance to start. Give it a try!
Aprons are back in style! Sew this simple half-apron in one hour, it's a fun and easy project.
Or, if you're really, really short on time, try out this beginner-friendly tutorial and make one in ten minutes. Worth trying out!
Have fun creating your unique tea cosy with your fabrics and colours!
These tea towels would be fantastic for gifts for your loved ones, neighbors, or friends. Make a few, and show them off in your kitchen!
A great combination: a table runner that doubles as a giant hotpad. Perfect for large family gatherings!
Every table needs cute napkins with cute napkin rings on them! One of the most useful projects for the home I've ever tried out. Saves tons of money, and you only need scraps of cotton fabric. Check it out.
Sew a simple, clear vinyl lunch bag. It is so practical to have one at hand in your kitchen for when you need to grab a snack and head outdoors - it also makes a great homemade present!
How cute is this spring table runner! A burst of color for your kitchen table.
I hope you've enjoyed the sewing projects showcased here. Craft ideas like these make super handy DIY gifts for friends, for housewarming parties, and for your own home decoration. These items are easy to make, functional, and might turn out to be the perfect way to spruce up your kitchen and make it feel like home. Your home.
Details make your home what it is. Make people wonder how you manage to make an ordinary spot on a kitchen counter so beautiful! Get your own pattern here.
Want something larger, or simply more variety? Add one more beautiful item to your mix: this 3-size Round Storage Basket Pattern. So sweet, and you can make one for every room in your home!


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MicrobiologyOpen Open Research
Public Documents (30)
Under Review (8)
Living in a bottle: Bacteria from sediment-associated Mediterranean waste and potenti...
Àngela Vidal-Verdú
Ocean pollution is a worldwide environmental challenge that could be partially tackled through microbial applications. To shed light on the diversity and applications of the bacterial communities that inhabit the sediments trapped in artificial containers, we analyzed residues (Polyethylene terephthalate (PET) bottles and aluminum cans) collected from the Mediterranean Sea by scanning electron microscopy and Next Generation Sequencing. Moreover, we set a collection of culturable bacteria from the plastisphere that were screened for their ability to use PET as a carbon source. Our results reveal that Proteobacteria are the predominant phylum in all the samples and that Rhodobacteraceae, Woeseia, Actinomarinales, or Vibrio are also abundant in these residues. Moreover, we identified marine isolates with enhanced growth in the presence of PET: Aquimarina intermedia, Citricoccus spp., and Micrococcus spp. Our results suggest that the marine environment is a source of biotechnologically promising bacterial isolates that may use PET or PET additives as carbon sources.
Plants as a realized niche for Listeria monocytogenes
Hoai-Nam Truong
Listeria monocytogenes is a human pathogen. It is the causative agent of listeriosis, the leading cause of bacterial-linked foodborne mortality in Europe and elsewhere. Outbreaks of listeriosis have been associated with the consumption of fresh produce including vegetables and fruits. In this review we summarize current data providing direct or indirect evidence that plants can serve as habitat for L. monocytogenes, enabling this human pathogen to survive and grow. The current knowledge of the mechanisms involved in the interaction of this bacterium with plants is addressed, and whether this foodborne pathogen elicits an immune response in plants is discussed.
Transcriptomic analysis of Streptococcus agalactiae periprosthetic joint infection
Hye-Kyung Cho
Although Streptococcus agalactiae periprosthetic joint infection (PJI) is not as prevalent as staphylococcal PJI, invasive S. agalactiae infection has recently increased in incidence. Here, RNA-Seq was used to perform transcriptomic analysis of S. agalactiae PJI using fluid derived from sonication of explanted arthroplasties of subjects with S. agalactiae PJI, with results compared to those of S. agalactiae strain NEM316 grown in vitro. 227 genes with outlier expression were found (164 up-regulated and 63 down-regulated) between PJI sonicate fluid and in vitro conditions. Functional enrichment analysis showed genes involved in mobilome and inorganic ion transport and metabolism to be most enriched. Genes involved in nickel, copper, and zinc transport, were upregulated. Among known virulence factors, cyl operon genes, encoding beta-hemolysin/cytolysin, were consistently highly expressed in PJI versus in vitro. The data presented provide insight into S. agalactiae PJI pathogenesis and may be a useful resource for the identification of novel PJI therapeutics or vaccines against invasive S. agalactiae infections.
Dynamic interspecies interactions and robustness in a four-species model biofilm
Aurélie Baliarda
Interspecific interactions within biofilms determine relative species abundance, growth dynamics, community resilience, and success or failure of invasion by an extraneous organism. However, deciphering interspecific interactions and assessing their contribution to biofilm properties and function remain a challenge. Here, we describe the constitution of a model biofilm composed of four bacterial species belonging to four different genera (Rhodocyclus sp., Pseudomonas fluorescens, Kocuria varians, and Bacillus cereus), derived from a biofilm isolated from an industrial milk pasteurization unit. We demonstrate that the growth dynamics and equilibrium composition of this biofilm are highly reproducible. Based on its equilibrium composition, we show that the establishment of this 4-species biofilm is highly robust against initial, transient perturbations but less so towards continuous perturbations. By comparing biofilms formed from different numbers and combinations of the constituent species and by fitting a growth model to the experimental data, we reveal a network of dynamic, positive, and negative interactions that determine the final composition of the biofilm. Furthermore, we reveal that the molecular determinant of one negative interaction is the thiocillin I synthesized by the B. cereus strain, and demonstrate its importance for species distribution and its impact on robustness by mutational analysis of the biofilm ecosystem.
The CCAAT-binding complex mediates azole susceptibility of Aspergillus fumigatus by s...
Chi Zhang
In fungal pathogens, the transcription factor SrbA (a sterol regulatory element-binding protein, SREBP) and CBC (CCAAT binding complex) have been reported to regulate azole resistance by competitively binding the TR34 region (34 mer) in the promoter of the drug target gene, erg11A. However, current knowledge about how the SrbA and CBC coordinately mediate erg11A expression remains limited. In this study, we uncovered a novel relationship between HapB (a subunit of CBC) and SrbA in which deletion of hapB significantly prolongs the nuclear retention of SrbA by increasing its expression and cleavage under azole treatment conditions, thereby enhancing Erg11A expression for drug resistance. Furthermore, we verified that loss of HapB significantly induces the expression of the rhomboid protease RbdB, Dsc ubiquitin E3 ligase complex, and signal peptide peptidase SppA, which are required for the cleavage of SrbA, suggesting that HapB acts as a repressor for these genes which contribute to the activation of SrbA by proteolytic cleavage. Together, our study reveals that CBC functions not only to compete with SrbA for binding to erg11A promoter region but also to affect SrbA expression, cleavage, and translocation to nuclei for the function, which ultimately regulate Erg11A expression and azole resistance.
Detection of antimicrobial resistance genes in urban air
Ágnes Becsei
To understand antibiotic resistance in pathogenic bacteria, we need to monitor environmental microbes as reservoirs of antimicrobial resistance genes (ARGs). These bacteria are present in the air and can be investigated with the whole metagenome shotgun sequencing approach. This study aimed to investigate the feasibility of a method for metagenomic analysis of microbial composition and ARGs in the outdoor air. Air samples were collected with a Harvard impactor in the PM10 range at 50 m from a hospital in Budapest. From the DNA yielded from samples of PM10 fraction single-end reads were generated with an Ion Torrent sequencer. During the metagenomic analysis, reads were classified taxonomically. The core bacteriome was defined. Reads were assembled to contigs and the ARG content was analyzed. The dominant genera in the core bacteriome were Bacillus, Acinetobacter, Leclercia and Paenibacillus. Among the identified ARGs best hits were vanRA, Bla1, mphL, Escherichia coli EF-Tu mutants conferring resistance to Pulvomycin, BcI, FosB, and mphM. Despite the low DNA content of the samples of PM10 fraction, the number of detected airborne ARGs was surprisingly high.
ON-rep-seq as a rapid and cost-effective alternative to whole-genome sequencing for s...
Gunn Merethe Thomassen
Identification, source tracking, and surveillance of food pathogens is a crucial factor for the food-producing industry. Over the last decade, the techniques used for this have moved from conventional enrichment methods, through species-specific detection by PCR to sequencing-based methods, whole-genome sequencing (WGS) being the ultimate method. However, using WGS requires the right infrastructure, high computational power, and bioinformatics expertise. Therefore, there is a need for faster, more cost-effective, and more user-friendly methods. A newly developed method, ON-rep-seq, combines the classical rep-PCR method with nanopore sequencing, resulting in a highly discriminating set of sequences that can be used for species identification and also strain discrimination. This study is essentially a real industry case from a salmon processing plant. Twenty Listeria monocytogenes isolates were analyzed both by ON-rep-seq and WGS to identify and differentiate putative L. monocytogenes from a routine sampling of processing equipment and products, and finally, compare the strain-level discriminatory power of ON-rep-seq to different analyzing levels delivered from the WGS data. The analyses revealed that among the isolates tested there were three different strains. The isolates of the most frequently detected strain (n=15) were all detected in the problematic area in the processing plant. The strain level discrimination done by ON-rep-seq was in full accordance with the interpretation of WGS data. Our findings also demonstrate that ON-rep-seq may serve as a primary screening method alternative to WGS for identification and strain-level differentiation for surveillance of potential pathogens in a food-producing environment.
Shining light on multi-drug resistant Candida auris: ultraviolet-C disinfection, wave...
Richard Mariita
Background: Candida auris is an emerging fungal pathogen of worldwide interest. It is associated with high mortality rates and exhibits increased resistance to antifungals. Ultraviolet-C (UVC) light can be used to disinfect surfaces to mitigate its spread. In this study, we analyzed the performance of different UVC wavelengths against C. auris to determine its wavelength sensitivity and UVC dose requirements and evaluated biofilm prevention dose requirements on most used materials in healthcare settings. Objectives: 1. To investigate UVC disinfection performances and wavelength sensitivity of C. auris; 2. To evaluate the UVC dose required for prevention of biofilm prevention on stainless steel. Methods: C. auris was grown following standard procedures. The study utilized six different UVC LED arrays with wavelengths between 252 and 280 nm. Arrays were set at similar intensities, to obtain doses of 5-40 mJcm-2 and similar irradiation time. Disinfection performance for each array was determined using log reduction value (LRV) and percentage reduction by comparing the controls against the irradiated treatments. Evaluation of the ability of 267 nm UVC LEDs to prevent C. auris biofilm formation was investigated using stainless steel, plastic coupons, and poly-cotton fabric. Results: Peak sensitivity to UVC disinfection was between 267 and 270 nm. With 20 mJcm-2, the study obtained LRV 3. On steel coupons, 30 mJcm-2 was sufficient to prevent biofilm formation, on plastic only 10 mJcm-2. A dose of 60 mJcm-2 reduced biofilms on poly-cotton fabric significantly. Conclusions: Results revealed that C. auris was most susceptible at 267-270 nm. Additional highlights from the study allow for the design and implementation of disinfection systems.
Polyunsaturated fatty acids cause physiological and behavioral changes in Vibrio algi...
David S. Smith
Vibrio alginolyticus and Vibrio (Aliivibrio) fischeri are Gram-negative bacteria found globally in marine environments. During the past decade, studies have shown that certain Gram-negative bacteria, including Vibrio species (cholerae, parahaemolyticus, and vulnificus) are capable of using exogenous polyunsaturated fatty acids (PUFAs) to modify the phospholipids of their membrane. Moreover, exposure to exogenous PUFAs has been shown to affect certain phenotypes that are important factors of virulence. The purpose of this study was to investigate whether V. alginolyticus and V. fischeri are capable of responding to exogenous PUFAs by remodeling their membrane phospholipids and/or altering behaviors associated with virulence. Thin-layer chromatography (TLC) analyses and ultra-performance liquid chromatography-electrospray ionization mass spectrometry (UPLC/ESI-MS) confirmed incorporation of all PUFAs into membrane phosphatidylglycerol and phosphatidylethanolamine. Several growth phenotypes were identified when individual fatty acids were supplied in minimal media and as sole carbon sources. Interestingly, several PUFAs acids inhibited growth of V. fischeri. Significant alterations to membrane permeability were observed depending on fatty acid supplemented. Strikingly, arachidonic acid (20:4) reduced membrane permeability by approximately 35% in both V. alginolyticus and V. fischeri. Biofilm assays indicated that fatty acid influence was dependent on media composition and temperature. All fatty acids caused decreased swimming motility in V. alginolyticus, while only linoleic acid (18:2) significantly increased swimming motility in V. fischeri. In summary, exogenous fatty acids cause a variety of changes in V. alginolyticus and V. fischeri, thus adding these bacteria to a growing list of Gram-negatives that exhibit versatility in fatty acid utilization and highlighting the potential for environmental PUFAs to influence phenotypes associated with planktonic, beneficial, and pathogenic associations.
Expression of degQ gene and its effect on lipopeptide production as well as the forma...
Lars Lilge
Bacillus subtilis is described as a promising production strain for lipopeptides. In the case of B. subtilis strains JABs24 and DSM10T, surfactin, and plipastatin are produced. Lipopeptide formation is controlled, among others, by the DegU response regulator. The activating phospho-transfer by the DegS sensor kinase is stimulated by the pleiotropic regulator DegQ, resulting in enhanced DegU activation. In B. subtilis 168, a point mutation in the degQ promoter region leads to a reduction in gene expression. Corresponding reporter strains showed a 14-fold reduced expression. This effect on degQ expression and the associated impact on lipopeptide formation was examined for B. subtilis JABs24, a lipopeptide-producing derivative of strain 168, and B. subtilis wild-type strain DSM10T, which has a native degQ expression. Based on the stimulatory effects of the DegU regulator on secretory protease formation, the impact of degQ expression on extracellular protease activity was additionally investigated. To follow the impact of degQ, a deletion mutant was constructed for DSM10T, while a natively expressed degQ version was integrated into strain JABs24. This allowed strain-specific quantification of the stimulatory effect of degQ expression on plipastatin and the negative effect on surfactin production in strains JABs24 and DSM10T. While an unaffected degQ expression reduced surfactin production in JABs24 by about 25%, a 6-fold increase in plipastatin was observed. In contrast, degQ deletion in DSM10T increased surfactin titer by 3-fold but decreased plipastatin production by 5-fold. In addition, although significant differences in extracellular protease activity were detected, no decrease in plipastatin and surfactin produced during cultivation was observed.
Comparison of swab types for collection and analysis of microorganisms
Natalie Wise
The human microbiome has begun to emerge as a potential forensic tool, with varied applications ranging from unique identification to investigative leads that link individuals and/or locations. The relative abundance of the combined DNA of the microbiome, compared to human nuclear DNA, may expand potential sources of biological evidence, especially in cases with transfer or low-copy number DNA samples. This work sought to determine the optimal swab type for the collection and analysis of the microbiome. A bacterium (Proteus mirabilis) was deposited by pipette onto four swab types (cotton, flocked, dental applicators, and dissolvable), and extraction and real-time PCR quantitation of the bacterial DNA were performed, which allowed for absolute microbial DNA recovery and comparison of yields across the four sampling substrates. Flocked swabs had the highest yield (~1240 ng) compared to the cotton swabs (~184 ng), dental applicators (~533 ng), and dissolvable swabs (~430 ng). The collection efficiency was further evaluated for cotton and flocked swabs using dried microbial samples spotted onto non-porous surfaces (treated wood, glass, plastic, and tile). Flocked swabs performed consistently better across wood, glass, and tile, but showed decreased recovery from plastic. The cotton swabs failed in the recovery of P. mirabilis DNA across all surfaces. Knowing the appropriate sampling substrate will be useful as others continue to investigate the use of the microbiome as a forensics tool.
Characterization of the genetic switch from phage phi13 important for Staphylococcus...
Camilla Kristensen
Temperate phages are bacterial viruses that either reside integrated in a bacterial genome as lysogens or enter a lytic lifecycle. Decision between lifestyles is determined by a switch involving a phage-encoded repressor, CI, and a promoter region from which lytic and lysogenic genes are divergently transcribed. Here we investigate the switch of phage phi13 from the human pathogen Staphylococcus aureus. phi13 encodes several virulence factors and is prevalent in S. aureus strains colonizing humans. We show that the phi13 switch harbors a cI gene, a predicted mor (modulator of repression) gene, and three high-affinity operator sites binding CI. To quantify the decision between lytic and lysogenic lifestyle, we introduced reporter plasmids that carry the 1.3 kb switch region from phi13 with the lytic promoter fused to lacZ into S. aureus and B. subtilis. Analysis of beta-galactosidase expression indicated that decision frequency is independent of host factors. The white "lysogenic" phenotype, which relies on expression of cI, could be switched to a stable blue "lytic" phenotype by DNA damaging agents. We have characterized lifestyle decisions of phage phi13, and our approach may be applied to other temperate phages encoding virulence factors in S. aureus.
Binding of the kringle-2 domain of human plasminogen to streptococcal PAM-type M-prot...
Olawole Ayinuola
M-protein (PAM) largely contributes to the pathogenesis of Pattern D Group A Streptococcus pyogenes (GAS). However, the mechanism of complex formation is unknown. In a system consisting of a Class II PAM from Pattern D GAS isolate NS88.2 (PAMNS88.2), with one K2hPg binding a-repeat in its A-domain, we employed biophysical techniques to analyze the mechanism of the K2hPg/PAMNS88.2 interaction. We show that apo-PAMNS88.2 is a coiled-coil homodimer (M.Wt. ~80 kDa) at 4°C - 25°C, and is monomeric (M.Wt. ~40 kDa) at 37°C, demonstrating a temperature-dependent dissociation of PAMNS88.2 over a narrow temperature range. PAMNS88.2 displayed a single tight binding site for K2hPg at 4°C, which progressively increased at 25°C through 37°C. We isolated the K2hPg/PAMNS88.2 complexes at 4°C, 25°C, and 37°C and found molecular weights of ~50 kDa at each temperature, corresponding to a 1:1 (m:m) K2hPg/PAMNS88.2 monomer complex. hPg activation experiments by streptokinase demonstrated that the hPg/PAMNS88.2 monomer complexes are fully functional. The data show that PAM dimers dissociate into functional monomers at physiological temperatures or when presented with the active hPg module (K2hPg) showing that PAM is a functional monomer at 37°C.
Characterization of promoters in archaeal genomes based on DNA structural parameters
Gustavo Martinez
The transcription machinery of archaea can be roughly classified as a simplified version of eukaryotic organisms. The basal transcription factor machinery binds to the TATA-box found around 28 nucleotides upstream of the transcription start site; however, some transcription units lack a clear TATA-box and still have TBP/TFB binding over them. This apparent absence of conserved sequences could be a consequence of sequence divergence associated with the upstream region, operonic and gene organization. Furthermore, earlier studies have found that a structural analysis gains more information compared to a simple sequence inspection. In this work, we evaluated and coded 3630 archaeal promoter sequences of three organisms, Haloferax volcanii, Thermococcus kodakarensis, and Sulfolobus solfataricus into DNA duplex stability, enthalpy, curvature, and bendability parameters. We also split our dataset into conserved TATA and degenerated TATA promoters in order to identify differences among these two classes of promoters. The structural analysis reveals variations in archaeal promoters' architecture, i.e., a distinctive signal is observed in the TFB, TBP, and TFE binding sites independently of these being TATA-conserved or TATA-degenerated. In addition, the promoter encountering method was validated with upstream regions of 13 other archaea, suggesting that there might be promoter sequences among them. Therefore, we suggest a novel method for locating promoters within the genome of archaea based on energetic/structural features.
Magnaporthe oryzae as an expression host for the production of the unspecific peroxyg...
Stefan Jacob
The filamentous fungus Magnaporthe oryzae has the potential to be developed as an alternative platform organism for the heterologous production of industrially important enzymes. M. oryzae is easy to handle, fast-growing and unlike yeast, posttranslational modifications like N-glycosylations are similar to the human organism. Here, we established M. oryzae as a host for the expression of the unspecific peroxygenase from the basidiomycete Agrocybe aegerita (AaeUPO). UPOs are attractive biocatalysts for selective oxyfunctionalization of non-activated carbon-hydrogen bonds. To improve and simplify the isolation of AaeUPO in M. oryzae, we fused a Magnaporthe signal peptide for protein secretion and set it under control of the strong EF1-promotor. The success of the heterologous production of full-length AaeUPO in M. oryzae and the secretion of the functional enzyme was confirmed by a peroxygenase-specific enzyme assay. These results offer the possibility to establish the filamentous ascomycete M. oryzae as a broad applicable alternative expression system. This is in particular valid for proteins that cannot or not in sufficient yields produced in established systems.
Attachment on mortar surfaces by cyanobacterium Gloeocapsa PCC 73106 and sequestratio...
Tingting Zhu
Cyanobacterial carbonate precipitation induced by cells and extracellular polymeric substances (EPS) enhances mortar durability. The percentage of cell/EPS attachment regulates the effectiveness of the mortar restoration. This study investigates the cell coverage on mortar and microbially induced carbonate precipitation. Statistical analysis of results from scanning electron and fluorescence microscopy shows that the cell coverage was higher in the presence of UV-killed cells than living cells. Cells preferably attached to cement paste than sand grains, with a difference of one order of magnitude. The energy-dispersive X-ray spectroscopy analyses and Raman mapping suggest cyanobacteria used atmospheric CO2 to precipitate carbonates.
Nitrite as a causal factor for nitrate-dependent anaerobic corrosion of metallic iron...
Takao Iino
Microbially influenced corrosion (MIC) may contribute significantly to overall corrosion risks, especially in the gas and petroleum industries. In this study, we isolated four Prolixibacter strains, which belong to the phylum Bacteroidetes, and examined their nitrate-respiration- and Fe0-corroding activities, together with two previously isolated Prolixibacter strains. Four of the six Prolixibacter strains reduced nitrate under anaerobic conditions, while the other two strains did not. The anaerobic growth of the four nitrate-reducing strains was enhanced by nitrate, which was not observed in the two nitrate-non-reducing strains. When the nitrate-reducing strains were grown anaerobically in the presence of Fe0 or carbon steel, the corrosion of the materials was enhanced by more than 20-fold compared to that in aseptic controls. This enhancement was not observed in cultures of the nitrate-non-reducing strains. The oxidation of Fe0 in the anaerobic cultures of nitrate-reducing strains occurred concomitantly with the reduction of nitrite. Since nitrite chemically oxidized Fe0 under anaerobic and aseptic conditions, the corrosion of Fe0- and carbon-steel by the nitrate-reducing Prolixibacter strains was deduced to be mainly enhanced via the biological reduction of nitrate to nitrite, followed by the chemical oxidation of Fe0 to Fe2+ and Fe3+ coupled to the reduction of nitrite.
Novel nitrite reductase domain structure suggests a chimeric denitrification repertoi...
Sarah Schwartz
Denitrification plays a central role in the global nitrogen cycle, reducing and removing nitrogen from marine and terrestrial ecosystems. The flux of nitrogen species through this pathway has a widespread impact, affecting ecological carrying capacity, agriculture, and climate. Nitrite reductase (Nir) and nitric oxide reductase (NOR) are the two central enzymes in this pathway. Here we present a previously unreported Nir domain architecture in members of Phylum Chloroflexi. Phylogenetic analyses of protein domains within Nir indicate that an ancestral horizontal transfer and fusion event produced this chimeric domain architecture. We also identify an expanded genomic diversity of a rarely reported nitric oxide reductase subtype, eNOR. Together, these results suggest a greater diversity of denitrification enzyme arrangements exist than have been previously reported.


It's a common question that's not easy to answer. Is it better to focus on long term or short term investments? Since each strategy has its own advantages and disadvantages, the better choice tends to vary according to your unique financial situation.
Short term investments, or temporary investments, are those which are expected to be converted into cash in less than a year. There are many short term investing options available to anyone, including stocks, money market funds, treasury bills and bonds.
A major advantage of short term investing is that your money is not tied-up for an extended period of time. If there are fluctuations in the market, you have the ability to change your position.
Short term investments often carry more risk, which offers the potential to earn more substantial returns. For example, stocks on the Dow Jones Industrial Index typically outperform low-risk certificates of deposit (CDs).
While it may take years for a corporate bond to mature, a stock portfolio is much more liquid. It's possible to increase your wealth in a short span of time, if there are gains in your financial positions.
Long term investments are assets that an individual or company plans to hold onto for more than a year. They could include stocks or bonds, real estate holdings or cash earmarked for later use.
Long term investments typically offer steady growth over many years or even decades. In addition to rises in stock prices, long term portfolios often increase in value through compounding, or the ability to reinvest profits over time.
Long range investments are usually much less volatile than their short term counterparts. For example, you might invest your money in a proven company that is likely to provide modest growth over many years.
If you are using a long term investing strategy, your number of transactions will decrease significantly, cutting the fees you pay to a financial institution.
The short answer is no. In investing, Financial planning all about finding the right balance. Each strategy has different expectations, satisfies different goals and carries different risks.
If you have questions about whether investing is right for you, schedule an appointment with a local financial advisor.


Thigh injuries are usually acute or sudden onset injuries. Muscle injuries such as groin strain, pulled hamstring (hamstring strain) and quad strain (thigh strain) are the more common thigh injuries. Thigh pain that comes on gradually is often an overuse injury such as groin tendonitis or inflammation.


An ultimatum (Latin: the last one) is a demand whose fulfillment is requested in a specified period of time and which is backed up by a threat to be followed through in case of noncompliance. An ultimatum is generally the final demand in a series of requests. As such, the time allotted is usually short, and the request is understood not to be open to further negotiation. The threat which backs up the ultimatum can vary depending on the demand in question and on the other circumstances.
The word is used in diplomacy to signify the final terms submitted by one of the parties in negotiation for settlement of any subject of disagreement. It is accompanied by an intimation as to how refusal will be regarded. English diplomacy has devised the adroit reservation that refusal will be regarded as an "unfriendly act", a phrase which serves as a warning that the consequences of the rupture of negotiations will be considered from the point of view of forcing a settlement. This opens up a variety of possibilities, such as good offices, mediation, the appointment of a commission of inquiry, arbitration, reprisals, pacific blockade and war.
Ultimatum is an American thrash metal band from Albuquerque, New Mexico.
Ultimatum was formed in 1992 in Albuquerque, New Mexico by Robert Gutierrez, John Carroll and Greg Dingess. The band's name is taken from concept of the ultimatum given by Joshua in Joshua 24:15 which states, "But if serving the Lord seems undesirable to you, then choose for yourselves this day whom you will serve." Robert's former Angelic Force guitar player Steve Trujillo joined soon after. Vocalist Scott Waters joined the band in January 1993 and their first public performance together was at the Sonshine Festival in Albuquerque, NM in 1993.
From 1992-1995 the band performed regularly locally and recorded three demos, Fatal Delay (1993), Symphonic Extremities by Ultimatum (1994), and then Symphonic Extremities (1995). The later two demos were later combined and released on CD by indie label Juke Box Media and released on CD with a new song that was mixed by Whitecross guitarist Rex Carroll.
to your scientific claims; there are things that can't be seen.
Look around at the beauty around you.
We are not an accident, there is meaning to this life.
dismissing creation as a myth, materialism is your kick.
Cold facts distorting the truth.
Fossil records donít hold up; Darwin's theory is a farce.
I am blinding. I am jaded. I am theory masked as fact.
I am blinding. I am jaded. All live underneath my shroud.
Lost identity, results of the big lie.
Moral decay the consequence of a world that's meaningless.
Play the fool, professing to be wise.


text:
Carousel Group to grow US presence
News, Operator
iGaming operator and B2C solutions provider Carousel Group has been awarded a sports betting licence from the Colorado Division of Gaming.
Carousel will offer its sports betting services to players via its flagship SportsBetting.com brand.
Bettors will have the opportunity to place money on sporting events both on desktop and via the SportsBetting.com mobile app.
The group is also working on expanding into other US states.
"Confident and optimistic"
SportsBetting.com will run on Carousel's in-house platform, which the group believes will enable them to "bring a unique and powerful product to the market".
Carousel Group CEO Daniel Graetzer shared his thoughts on the Colorado licence approval, which were as follows.
"Despite the ongoing and tragic global crisis, we couldn't be more confident and optimistic about the sports industry and we're very excited to be bringing our flagship brand to the American market.
"We have built our business upon a team with years of experience in the industry combined with our own technology, which gives us a huge advantage over the competition.
"It's in our DNA to be innovative and with our own technology we will offer something fresh, exciting and very customer oriented to the U.S. betting community."
SportsBetting.com will also have a dedicated affiliate programme.
"Emerging, dynamic and technologically-driven"
To launch SportsBetting.com into the Coloradan market, Carousel teamed up with Johnny Nolon's Casino – a venue based in Cripple Creek.
Managing Director David Minter commented on the partnership with Carousel and sports betting in Colorado. He said the below.
We are delighted to be partnering with such an emerging, dynamic and technologically driven company such as Carousel Group and are very excited about this partnership.
"Johnny Nolon's Casino was the first casino to open for casino gambling in Cripple Creek on Oct 1, 1991 and we are looking forward to now finally being able to offer sports betting."
On its website, Carousel said that it plans to announce expansion into other US states soon. It will also be opening new offices in both New Jersey and Colorado later this year.
Colorado is approaching the end of its first month of regulated sports betting, after last November's referendum narrowly swung in its favour.
Carousel Group, Colorado, SportsBetting.com, US iGaming
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Snow kiting combines the airfoil and technique used in kitesurfing with ski or snowboard equipment for gliding on lakes or open fields.
Kites for sporting activities including kite skiing.
Power kites for power sports.
Colorado snowkiting, kite skiing and kit snowboarding. Products sales and instruction.
Power kite specialists. Store located in Worcester, Worcestershire.
Snow and power kite manufacturers based in France.


text:
Q: Array of images is not showing proper without alert box on canvas with x , y coordinates I have got image names in loop from XML file using ajax and showing on HTML canvas with X, Y coordinates. But images is not show proper without alert box. Means If I put alert box then it showing fine but if I remove alert box from code then all images showing on last X, Y coordinates.
My HTML code is -
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redpajama_set_name: RedPajamaStackExchange


The Bears secured a commitment from Higley (Gilbert, AZ) High School quarterback Spencer Brasch during his official visit to Cal Sunday and Monday. A dual threat QB, Brasch competed 62% of his passes on the season for 33 touchdowns through the air with five intereceptions along with 898 yards and 13 touchdowns on the ground, averaging an impressive 9.9 yards per carry.
"I always wanted to be in California," said Brasch. "So it was perfect that I got an offer from Cal.
"Great school, good program and really nice area and beautiful campus. I'm really looking forward to the education I'll get at Cal and being a part of this program.
"One of the things I like a lot, too, is how nice the coaches are. Coach Tui was super-excited for me and I like all the other coaches, too. It felt like I was really welcomed right away and really a part of the family at Cal."
"I think he is a really good quarterback that also happens to be a very good athlete," said Higley head coach Eddie Zubey. "And a lot of times you see kids that are really good athletes that happen to be able to throw the ball, but he's a quarterback first, but also has the ability to beat you with his feet and just be aware. He's a great athlete."
To say Brasch is a multi-sport athlete would be more than an understatement.
"Oh yeah, I say, which sport doesn't he play," said Zubey. "As a sophomore last year, he went out for the baseball team. He was a varsity pitcher. He was clocked at 86 miles an hour as a sophomore.
"He always runs track. Track and baseball is the same season here. So he's playing both sports at the same time. In his sophomore year he made it to the state track meet in the triple jump and the open 400."
"This year he decided he didn't want to do baseball, so he still did track again for his junior year and then he decided to join our volleyball team. Our volleyball team won state. And he'd never played volleyball before, but it only took like a game or two to get it down. He's the outside hitter.
"A week before the regular season ended, he stopped playing volleyball because of the football stuff. Spring ball started picking up. It started to get more evident that, you know, college football was his calling. So no matter what he does, he's great.
"This year he made it to the state track meet in the 4x100, the 4x400 and he was the anchor on both of those, as well as the triple jump.
"He's also our punter. He averages 42 yards a punt. He was the emcee of our spring assembly, too. You know, up in front of all the students. He's just a good kid. Great family, he's a superior athlete."
The talented Brasch saw his first varsity time as a young frosh for Higley.
"After his freshman regular season," said Zubey. "He came up for the playoffs. He played a couple series as a freshman I believe. As a sophomore, Spencer was our starting varsity quarterback. He also played free safety on JV. So we pulled him up to varsity. We had three year starter on varsity. After the first game of the playoffs, Spencer was our starting free safety. He was just a ball hog. You know, he's 6-3/190 and just a great athlete. He beat the kid out at free safety in about two weeks."
The program had several high level players in Brasch's sophomore season but after they moved on, Brasch took on more of a leadership mantel last season.
"This year he's really stepped up his leadership role," said Zubey. "About two weeks after the season ended, he got all the guys together and they started organizing, going and throwing on their own. We didn't talk to him about doing any of that stuff. I'd rather him wait until January, get a little bit of time off, but he wanted to get them working right away. So he's been out there with those guys. They're throwing Saturday mornings on their own. It's been great. The kids really respect him. They respond to him.
"He's a winner. He's only lost three games in high school. His freshman year, we lost one game. He went undefeated as a sophomore. Last year as a junior, we only lost two games. Ironically enough, all three of his losses are to the same school, Saguaro, which is always one of the top programs around here, with 16 D1 athletes.
A dual threat QB, Brasch competed 62% of his passes on the season for 33 touchdowns through the air with five intereceptions along with 898 yards and 13 touchdowns on the ground, averaging an impressive 9.9 yards per carry.
"He's a legit dual threat," said Zubey. "The kid has a super strong arm. He could probably throw the ball through a brick wall. There's not a throw on the field he can't make. He's got huge hands. That's always a comment that I get when college coaches shake his hand. He's got big 'ol paws.
"Another good thing for Spencer is, he didn't throw his first interception until game ten," said Zubey. "And it was on a tipped running back's screened pass. He threw four picks on the year and only one of them were legit his fault. One was on a tipped running back screen and then he threw one late over the middle. I think he threw 34 touchdowns and four interceptions. The first one didn't come until week ten. So he really took care of the ball. I don't think he fumbled it until the playoffs. He just did a good job of taking care of the football."
Cal special teams coach Charlie Ragle played a key role in identifying and recruiting Brasch, with his extensive connections in Arizona -his recruiting territory with Cal.
"Charlie and I go back a long time," said Zubey. "I played at ASU. Then I GA'd at ASU and then we coached against each other in high school. We were head coaches, when he was at Chaparral. And then he became a GA at ASU for a year. So we've definitely crossed paths. He does a great job.
"Also, he's been on our guy Ty Robinson as well since Ty was a freshman. He was always saying, 'This kids' going to be special'" Lo and behold, he's one of the top recruits in the nation. He does a great job with the kids. He builds relationships with them and when he's there they can tell he's genuine. He doesn't pull any punches with them."
Both Ragle and offensive coordinator Beau Baldwin were there to watch Brasch throw before his offer and meet with Brasch and Zubey.
"It was my first time meeting Coach Beau (Baldwin). It was great. We got to sit down and talk a little bit before just about everything. And having Ragle in the room as well kind of lightens the mood and opens him up a little bit more as well. They were great to talk to. They were honest. We got on the board a little bit, talking a little offense and that was fun to do. So, it was good."
SMU, NC State and Vanderbilt were top contenders for Brasch before his commitment to Cal.
I appreciate 46 tds and 5 ints.
He had me at 2:11 of that hudl video.
Fantastic! Welcome QB Spencer Brasch.
Cal beat out their counterpart in Davis for two football recruits on the same day!
We should lock up Ragle for the future. This is his class.
Nice footwork setting in the pocket.
Good judgement in the RPO game.
Some nice passes while scrambling.
Nice elusiveness and speed when running.
Arm strength looks decent --- but lots of his passes were to wide open receivers behind the defense. We'll need to see how he is when he has to look off defenders and make tighter passes.
can run as well as pass!
"He could probably throw the ball threw through a brick wall." But I digress.
Spencer is an amazing athelete. What I like most about his background is the leadership position he's taken on his team, and his willingness to take on any role his coaches ask him to fulfill. He could be our first "three-way" player: QB, Safety and Punter.
Then he could pitch for the BB team, and drop in on the volleyball team for big matches. Oh, and win the Pac-12 triple-jump in track during the Spring.
All while getting top marks academically.
Our ability to successfully recruit a player this talented speaks to the huge progress our coaching staff is making in recruiting.
Just curious. Is there anything Cal can do that you would approve of?
If he ends up being very good for us, which joke will we hear more of: Brasch Balls or Bill Braschy?
Where's the genius that wants Tui gone? Lol. I love our genius commentators that specialize in buttplay and moonlight as college football coaches.
What's wrong with buttplay? Also, every single person on this site is an expert on college football and basketball, at least.
Buttplay? Well there's a well reasoned post.
Just curious. What is wrong with his post?
He's got huge hands. That's always a comment that I get when college coaches shake his hand. He's got big 'ol paws.
"Big 'ol paws." No more small hands jokes for our QBs.
Welcome to Berkeley. Go Bears!
Sweet! Way to go Bears!
Wow! Well, that's a really nice vid. He sure does make some nice throws, and his running is impressive. And even with his punting.....I get the impression he hasn't reached his potential punting yet. If he gets just a bit more extension out of that leg, he could add a yard or 2 or 3+. Seems like a terrific young man with great statements about his character. I'm jazzed.
I don't recall any previous quarterback we recruited in recent memory that was described this way.
Nothing it all. But you gotta respect the specialists. The dudes that roll up their sleeves and REALLY get in the mud. We are lucky to retain a number of said specialists.
I think Brasch is a great get. Could be great at QB, but even if he is beat out by someone else could play safetyor a situational punter on 4th down, with run, pass and punt (providing an 11th on coverage) all an option.
Ken loves our Buttplay guys, that's cool bro.
No disrespect to Spencer but I'm pretty sure that VicV, Gilliam, and McIlwain were/are all described that way.
Mark me as very impressed.
1. Strong arm on long and medium range passes.
2. Good field vision and pocket presence.
3. Strong and savvy runner.
Only negative appears that he might be a little too Colin Kapernick with the run. But I did notice a few plays where he kept his eyes down field when pocket protection broke down instead of his taking off. And his runs usually looked like designed plays.
I think Maynard was described this way or was it Ayoob?
Hey thanks for all the kind words about Spencer Brasch. I'm a proud Pappa Bear at this point. I look forward to many "spirited" conversations with all of you! GO BEARS!!
Right you are. Mea Culpa. But if we did . . .
Nothing beats seeing one of your kids do well, does it? A lot of hard work on Spencer's part.
I think for him, the best part of being at Cal may well turn out to be the opportunity to connect with outstanding faculty. It's hard to think of an area where Cal does not have world class faculty & research.
Welcome to Cal too, DB.
Congratulations and welcome! Glad to have your son on board hope you get many opportunities to visit the Bay and see your son play.


During the last year when meeting my friends for a glass of wine before dinner, we always ended up choosing Il Santino. The little enoteca Il Santino is a much smaller sibling of the famous restaurant Santo Bevitore, just next door. The wine bar is so tiny that it easily gets crowded during the hours between work and dinner. It's not easy to get a seat but there's also the counter and many choose to stand inside or outside while tasting a nice glass of wine. For me it's worth the wait since I enjoy the mood, wine and food.
Whether you choose to sit inside, enjoying the chatting, music and relaxed atmosphere or sit outside at one of the small tables when it's warm, Santino is always a good idea. The wine list is long and you get a tasty little nibble with your glass. Perfect to come in before dinner for an intimate glass for two or with a group of friends. Sometimes we end up staying to have something from the great, but more limited, menu and continuing to enjoy nice wines until late at night.


text:
HCC Policies and Procedures
What is Title IX
Report/Complaint Process
(current)Contacts and Resources
Contacts and Resources
Helpful Resources: Maintaining the health, safety, and well-being of the campus community is HCC's primary concern. Below is a list of campus and community resources:
HCC Department of Public Safety (Library): 443-412-2272
The Department of Public Safety office is open 24 hours a day to assist victims of sexual misconduct. If an individual is not certain whether criminal conduct is involved, a Public Safety Officer can assist in determining whether a crime has occurred. With the victim's consent, the Public Safety Officer may notify the Harford County Sheriff's Office to conduct an official criminal investigation of any allegations of sexual misconduct. With the victim's consent, the Public Safety Officer will arrange for transportation to University of Maryland Upper Chesapeake Medical Center where a SAFE exam can be performed by a trained FNE.
Harford County Sheriff's Office: 911 or 410-838-6600
Any individual who experiences sexual misconduct is strongly encouraged to contact local law enforcement.
Harford County Sheriff's Office/Victim Services: 410-836-5490
HCC Emergency Phones:
Emergency phone lines are installed in all classrooms. The phones will dial directly to Public Safety by pressing the corresponding speed dial, but 9-1-1 has to be manually dialed.
Title IX Coordinator and Deputy Coordinators:
Dr. Jacqueline Jackson
Vice President for Student Affairs and Institutional Effectiveness
Title IX Coordinator
HCC – Executive Suite, 3rd Floor of the Library
jajackson@harford.edu
Ms. Nicole Hoke-Wilson
Director for Disability and Student Intervention Services
Deputy Title IX Coordinator
HCC – Student Center
nhokewilson@harford.edu
Ms. Pamela Stell
Director for Human Resources and Employee Development
HCC – Chesapeake Center
pstell@harford.edu
Ms. Elizabeth Mosser
Associate Dean of Academic Operations
HCC – Executive Suite
emosser@harford.edu
Mr. Estevan Vasquez
HCC – Susquehanna Center
evasquez@harford.edu
Mr. Sean Wright
Fitness Center Manager
swright@harford.edu
Ms. Leslie Manning
Transfer Admissions Advisor
lmanning@harford.edu
Additional Resources and Support:
HCC Escort Service
The HCC Department of Public Safety operates an escort service at all times for use by those concerned about their safety while on campus. Call Public Safety at 443-412-2272 or ext. 2272 from a HCC phone to request a walking escort to your vehicle. Wait in a well lit or populated location for the arrival of the uniformed Public Safety Officer. Also visit the Department of Public Safety website for Crime Prevention tips.
HCC Student Intervention and Prevention Team:
Students impacted by sexual misconduct can access this service for resources and referrals that may provide support by calling 443-412-2132.
HCC Student Conduct and Intervention Specialist : 443-412-2073
Upper Chesapeake Medical Center, SAFE Coordinator: 443-843-5000, ext. 5337
Sexual Abuse/Sexual Assault Resource Center (SARC): 410-836-8431 or 410-836-8430 (24-Hour Hotline)
SARC provides confidential domestic violence and sexual assault services in Harford County. Services include: a safe house for victims of domestic violence and their children; legal services; individual and group counseling for victims and children; an abuser intervention program; crisis intervention; community outreach; education and professional training; and court and hospital accompaniment. Visit their website, www.sarc-maryland.org
Cecil County Domestic Violence/Rape Crisis Center: 410-996-0333
The Department of Social Services provides counseling to survivors and children, shelter, sexual assault services, and court and hospital accompaniment by advocates.
TurnAround, Inc.: 443-279-0379
A domestic violence and sexual assault center in Baltimore, MD, that helps women, children, and families who are victims of abuse. Visit their website, www.turnaroundinc.org
Family Crisis Center of Baltimore County: 410-828-6390
This center provides essential and life-saving services to families experiencing conflict and violence. Visit their website, www.familycrisiscenter.net
Rape, Abuse & Incest National Network (RAINN): Hotline: 1-800-656-HOPE or www.rainn.org
Safe Zone:
Support at HCC for LGBTQ+
It has been, and will continue to be, the policy of Harford Community College that all students, employees, applicants, and other persons dealing with the College will do so in an atmosphere that is free from discrimination on the basis of race, color, religion, sex, national origin, age, status as an individual with a disability, veteran, sexual orientation, gender identity or expression, marital status, genetic information or any other status protected by law. This policy includes, but is not limited to, decisions about recruitment, hiring, training, promotion, compensation, benefits, transfers, social or recreational programs, academic opportunities and enrollment.


This was developed by The Institute for Innovation and Improvement as part of their Safer Care programme, to encourage effective communication. Situation, Background, Assessment, Recommendation (SBAR) is a structured method for communicating critical information that requires immediate attention and action.
This training guide describes the SBAR approach, and how to lead an implementation of the SBAR approach in the workplace.


An imminent whirlwind tour of North and South America awaits the two Renault-engined Formula teams in Formula 1, Red Bull and Toro Rosso, with three grands prix in as many weeks – starting with the USA GP in Austin, Texas this Sunday (25 Oct).
A fortnight later comes the next race on the F1 calendar, the season's penultimate round at the terrific Interlagos circuit in Sao Paulo, Brazil.
For British fans the races will take place at the following UK times: USA 7.00pm; Mexico 7.00pm; Brazil 4.00pm.
Red Bull and Toro Rosso have suffered mixed fortunes in the most recent two grands prix, at Suzuka, Japan and Sochi, Russia. Suzuka's race saw Max Verstappen and Carlos Sainz finish in ninth and tenth places for Toro Rosso – as in the previous GP in Shanghai, both drivers again caught the attention of the fans and TV viewers by charging past a number of rivals.
Sochi's GP saw Red Bull driver Daniil Kvyat score a solid sixth place finish in front of his home crowd as Verstappen again was left to charge up the order after an early mishap. He finished tenth for the final point. Both Sainz and Kvyat's team-mate Daniel Ricciardo had to retire from the race (the latter when running fourth). Ricciardo had also been unfortunate in Japan when an opening lap puncture put him a lap behind the leaders.


I prepared this blog yesterday on Laura's birthday and then I thought it was probably a little self indulgent…Me as a dad flooding social media with pics of my daughter on her birthday. Melanie (wife) asked where was the blog that I prepared on Friday and I made excuses for not posting it.
But then I thought….What if more dads did that. I have some understanding of the power of a dad's love. I have a dad who loved me. And I am a dad loving my two princesses. And I have to say….I love having a dad role in some princesses' who no longer have a dad or who have a defunct dad, or don't know their dad.
And I also thought…..I don't care if no one looks at this blog, because it is posted for an audience of one. Just Laura. My Laura. The love of my life. Hey Princess, your dad loves you completely…So So proud of the way you do life. Happy Birthday.
‹ Wyndham Vacation Clubs: A Huge Ripoff or A Sound Investment?
Great stuff Peter! You are a good Dad and man of God.
You are a fantastic dad to your 2 daughters Lakeisha & Laura. Also to many other daughters that you have taken into your heart as a father figure. Be proud you deserve to brag about your daughters.


This message originally came from the Berkeley PTA Council and has been approved for sending to the entire community by Principal Beth Rhine. If you plan to attend, please also be mindful about getting your student to school on time!
Before work or school this Friday, November 18, from 7:00–8:00 am, please join in a community celebration of Berkeley's shared values in Civic Center Park at 2100 M.L. King Jr. Way, to watch the sun rise and stand together. Breakfast provided; wear your best tie-dye!
Mayor-elect Jesse Arreguín and other community leaders will address the crowd on the topics of unity, positive action and Berkeley's hallowed tradition of counterculture. Let's let all of our precious children, students and community members know that in Berkeley, we rededicate ourselves to a fair, progressive, inclusive and sustainable world.
This is a celebration! In the wake of the national election, let's join to reaffirm our deeply held values of inclusiveness, diversity, human rights, equity, environmental action, peace and love. Take part in the creation of a giant human peace sign by standing together in the park as the sun rises.
We invite all neighborhood organizations, faith communities, schools, organizations, and businesses to join in—all of Berkeley. Let's show the rest of the country that we will forward our values with renewed energy, urgency and unity.


VisitaPrint is one of my favorite online shops! I love my custom $2 t-shirt! And starting today they are offering FREE labels!
4 Days Only! – July 26-29!
140 FREE Return Address Labels! The labels are free – just pay shipping and processing! With over 7,000 of designs to choose from, these labels are extremely versatile. You are sure to find a design that suits your style. Unsure where to start? Sort by style or theme or search for a specific color or image!
I ordered labels for school supplies for my daughter on Tuesday. I received an email yesterday that they are on their way. Shipping was just $3.03!


MINGO JUNCTION — With the news Thursday of as much as a $500 million investment in the former Wheeling-Pittsburgh Steel Corp. and a possible 500 new jobs, residents of Mingo Junction are looking forward to the future and a return to the Mingo Junction of the past.
And the mayor is right, as residents at the Mingo Senior Citizens Center and the Parkview Inn had lots to say Friday.
Officials of JSW Steel, a leading steelmaker based in India, announced their plans and signed certificates with Secretary of Commerce Wilbur Ross Thursday during the SelectUSA Investment Summit at National Harbor, Md.
JSW initially will spend $250 million to restart the electric arc furnace and refurbish the hot strip mill and slab caster.
If market conditions support it, a second phase would include another investment of $250 million for a second EAF and additional hot strip mill equipment, pending the outcome of feasibility studies and permit approvals, the steelmaker said.
Local resident Fred Pernick agreed.
Village Administrator Bob Smith said a new and improved village is certainly on the way.
Smith's sentiments were shared by state Sen. Frank Hoagland, R-Mingo Junction.
"This week's announcement by JSW Steel means more jobs, more economic development and more opportunity for Southeast Ohio. We are seeing firsthand the impact the Trump administration, combined with the Ohio Senate's policies reducing regulatory red tape, are having on our state's manufacturing jobs," Hoagland said.
And, Edward "Bibo" Potenzini believes this is just what his hometown needs to bring life back into the village.
"We were down for so many years. We were scraping by for a long time," he noted.
Village resident John Buchmelter summed up the feelings of most of the community.
Thursday's announcement drew the praise of U.S. Rep. Bill Johnson, R-Marrietta, who said it was good news for the region.
During the announcement ceremony, Secretary of Commerce Wilbur Ross singled out work by JobsOhio and the Appalachian Partnership for Economic Growth for their work on the JSW project.
Ed Looman, project manager for APEG, said activity grew during the past few weeks after an initial meeting by JobsOhio with JSW a few months ago.
He said members of the JSW team met with him, Evan Scurti, executive director of the Jefferson County Port Authority, and representatives of JobsOhio met Tuesday on site at the mill.


Jardin la Nurserie/The Nursery Garden is a collaborative artwork by SUPERFLEX, made for three public hospitals in the French islands of Réunion and Mayotte located in the Indian Ocean. The project aims to facilitate an exchange of knowledge between different cultures of medicine, challenging the common boundaries between modern and traditional medicine - reintegrating plants, the 'traditional', into the confines of 'modern' hospitals.
In each hospital courtyard, a plant nursery and a surrounding garden are built, containing medicinal plants growing on each island. The nursery gardens provide a neutral setting where patients, visitors and hospital staff can meet each other out of their usual habitat. An adaptable design favours this versatility: wooden structures that can be opened and closed, transitioning from a protective intimate greenhouse to a broad public agora. A participative space, where everyone is invited to contribute as a volunteer or by bringing new plants to the collection. As the plants grow larger, they are replanted onto the outer hospital grounds. As the Living Archive grows, local culture and knowledge of the medicinal plants is reinforced and maintained for future generations. As the project grows on the two islands, a bond between the distinct cultures of Mayotte and Réunion is created.
Jardin la Nurserie/The Nursery Garden was developed with local hospital representatives acting under 'Les Nouveaux Commanditaires', a joint initiative by Fondation de France. Designed in collaboration with Rasmus Koch Studio and landscape architect Sébastien Clément. Partners of The Nursery Garden are: Directorates of Cultural Affairs of Réunion and Mayotte, the Regional Agency for Indian Ocean Health, The University Hospital Centre of Réunion and the Hospital of Mayotte, Daniel & Nina Carasso Foundation & Fondation de France.


The Get Out Game - Are You Ready?
The Get Out Game – Are You Ready?
What is The Get Out Game?
This Cyber Weekend Berghaus is doing things differently with The Get Out Game – a journey into the outdoors that will earn you lasting memories and Berghaus prizes.
Interested? Let us tell you more….
From sunrise to sunset on Saturday 26th November Berghaus crews will be hiding extraordinary things in four extraordinary locations across the UK by planting flags for you to discover.
The challenge? Find the Flag. Earn the Reward.
There'll be up to 1000 rewards hidden in the mountains including Extrem gear, for the first to find the flag, along with a mix of our favourite tees and hats and gloves. We'll also be giving a pre-loaded gift cards away to every adventurer who takes on the challenge of hunting down our crews' location. Sounds fun, right?
Cool! How do I get involved?
To get your hands on a prize all you need to do is find one of the four Berghaus flags planted across the UK. We'll have 250 prizes in each location up for grabs, so make sure you get there early as once they're gone our crew will be packing up and hiking home.
To find the flag simply sign up here, select the area closest to you and await your clue. Then you just need to suss out the location and get outside to earn your reward.
Clues will be revealed on Black Friday, so this year, cut out the Cyber Weekend chaos and join in The Get Out Game – a journey into the outdoors which could earn you unforgettable memories and Berghaus prizes.
Safely does it. Scramble with care so not to put yourself (or anyone else) in any danger to reach our crew.
Supplies are a must so take your wrappers on the journey with you to leave no trace on the trail.
Who knows what the weather will throw your way (rain, probably!). Gear up so that you're warm, dry and comfy.
When it comes to rewards – once they're gone, they're gone. So… get started on de-coding your clue as soon as it arrives.
There are 250 rewards at each location and we're dishing them out on a first come first served basis. Here's what you can expect to find – that's provided you find us, of course.
Online maps and local tourist websites may be your friend in deciphering the clue. Print maps or write down the directions as to where you are going – who knows if there'll be phone signal where we're hiding out.
You can contact us on getoutgame@berghaus.com – be warned though, we'll be keeping tight-lipped about our exact locations.
Not sure your heading to the right location? Keep one eye on our website and social feeds like facebook and twitter for helpful hints throughout the day – as we'll be sharing pictures of the crew as they get found!
The Get Out Game. Find the Flag. Earn the Reward.
So, will we see you there?
More details about the event including full terms and conditions can be found here.