text:
Advances in Brief
PRL-3 and PRL-1 Promote Cell Migration, Invasion, and Metastasis
Qi Zeng, Jing-Ming Dong, Ke Guo, Jie Li, Hui-Xian Tan, Vicki Koh, Catherine J. Pallen, Edward Manser and Wanjin Hong
Qi Zeng
Jing-Ming Dong
Ke Guo
Jie Li
Hui-Xian Tan
Vicki Koh
Catherine J. Pallen
Edward Manser
Wanjin Hong
DOI: Published June 2003
We demonstrate here that Chinese hamster ovary cells stably expressing PRL-3, a Mr 20,000 prenylated protein tyrosine phosphatase, or its relative, PRL-1, exhibit enhanced motility and invasive activity. A catalytically inactive PRL-3 mutant has significantly reduced migration-promoting activity. We observe that PRL-3 is associated with diverse membrane structures involved in cell movement. Furthermore, we show that PRL-3- and -1-expressing cells, but not control cells, induce metastatic tumor formation in mice. Thus, our results deliver the first evidence for a causative role of PRL-3 and -1 in promoting cell motility, invasion activity, and metastasis.
PRL-1 4 was originally identified as an immediate early gene of which the transcript is induced during liver regeneration after partial hepatectomy (1) . Searching expression sequence tagged databases with the PRL-1 sequence led us to identify PRL-2 and -3 (2) . The three members share 76–87% amino acid sequence identity. These PRLs represent a novel class of PTP with a unique COOH-terminal prenylation motif. Although PRL-1 was originally described as a nuclear protein (1) , our analysis of heterologously expressed PRL-1, -2, and -3 has shown that these phosphatases are mainly associated with the plasma membrane and endosomal structures in a prenylation-dependent manner, and the majority of endogenous PRL-3 in intestine epithelial cells is also non-nuclear (3) . These data suggest that the major sites of action of the mature, post-translationally prenylated PRL-PTPs are at the membrane.
Many genes have been implicated in the development and progression of colorectal cancer (4 , 5) . However, the identification of consistent genetic alterations associated with the transition from the primary tumors to metastatic colorectal liver disease has proved elusive. Gene expression profiling revealed recently that among 144 up-regulated genes detected in metastatic colorectal liver samples, PRL-3 is the only gene consistently overexpressed in all 18 of the cancer metastases examined, with essentially undetectable PRL-3 expression in normal colorectal epithelia and intermediate expression in advanced primary cancers (6) . The overexpression of the PRL-3 transcript is because of gene amplification in 3 of 12 metastases examined, whereas enhanced transcriptional activity likely accounts for elevated PRL-3 transcripts in the other metastases. This study suggests the possibility that an excess of PRL-3 phosphatase is a key alteration contributing to the acquisition of metastatic properties of the tumor cells, but cell biological and mechanistic evidence supporting such a role is lacking. In this study we provide evidence to support a causal role of PRL-3 and -1 in tumor metastasis.
Generation of Stable CHO Cell Lines Expressing Myc-PRL-1, -3, and β-Gal.
To introduce the Myc epitope (10 amino acid residues) at the NH2 termini of PRL-1 and -3, a PCR-based approach was used. Forward primer A incorporating the Myc epitope (italicized; 5′-gc gaattc acc atg gag cag aag ctg atc tcc gag gag gac ctc gct cga atg aac cgc cct gct c-3′) and reverse primer B (5′-gt ggatcc tta ttg aat aca aca gtt g-3′) were used to amplify PRL-1 cDNA. Forward primer E incorporating the Myc epitope (5′-gc gaattc acc atg gag cag aag ctg atc tcc gag gag gac ctc gcc cgc atg aac cgg cct gcg cct g-3′) and reverse primer F (5′-ct ggatcc cta cat gac gca gca tct ggt c-3′) were used to amplify PRL-3 cDNA. The PCR fragments were digested with EcoRI and BamHI, and inserted into the inducible expression vector pStar (7) . CHO-K1 cells (American Type Culture Collection, Manassas, VA) were used to generate CHO cell lines stably expressing Myc-PRL-1 (clone 9), Myc-PRL-3 (clone 36), or β-gal (clone 8 and 13), as described previously (3 , 7) . Briefly, the cells were transfected with the above pSTAR-Myc-PRL-1, Myc-PRL-3, or β-gal plasmids, and cultured in RPMI 1640 supplemented with 10% FBS and selected in 1 mg/ml of neomycin. The c-Myc antibody (9E10) was from Santa Cruz Biotechnology (Santa Cruz, CA).
Generation of CHO Cells Stably Expressing EGFP-PRL-3 and EGFP-PRL-3 (C104S).
Synthetic oligonucleotides were purchased from Oligos Etc. (Wilsonville, OR). The Pyrococcus furiosus DNA polymerase was from Stratagene (La Jolla, CA). The pEGFP-C1 vector was from (Clontech). 5 To prepare a plasmid (pEGFP-PRL-3) for expression of PRL-3 NH2-terminally tagged with EGFP (EGFP-PRL-3), forward primer 1 (5′-gtg aat tct atg gcc cgc atg aac cgg-3′), and reverse primer F (above) were used to retrieve the PRL-3 coding region by PCR. The PCR reaction was resolved by agarose gel electrophoresis, and the specific fragment (530 bp) was eluted and purified. The fragment was digested with EcoRI and BamHI, and then inserted into the corresponding sites of the pEGFP-C1 vector. To construct pEGFP-PRL-3 (C104S) containing an inactivating mutation of the essential catalytic cysteine residue to serine at position 104 in the phosphatase active site, forward primer 1 in combination with reverse primer 3 incorporating the desired nucleotide (C to G, italicized) substitution (5′-cag gcc cgc cac aga gtg cac aag-3′) were used to retrieve the coding region for the NH2-terminal portion of PRL-3 (N-fragment). Forward primer 4 incorporating the desired nucleotide (G to C, italicized) substitution (5′-ctt gtg cac tct gtg gcg ggc ctg-3′) and reverse primer F (above) were used to retrieve the coding region for the COOH-terminal portion of PRL-3 (C-fragment). The gel-purified N-fragment and C-fragment were mixed and used in a PCR reaction with forward primer 1 and reverse primer F to generate the entire cDNA encoding the mutant PRL-3 (C104S). After digestion with EcoRI and BamHI, the specific fragment (530 bp) was purified and cloned into the corresponding sites of pEGFP-C1 vector. The two expression constructs were confirmed by DNA sequencing and transfected into CHO-K1 (ATCC CCL-61) cells using Lipofectamine 2000 (Invitrogen). The cells were cultured in RPMI 1640 supplemented with 10% FBS and selected in 1 mg/ml of neomycin for 20–30 days to establish stable cell pools.
Cell Motility Assay.
This was performed as described (8) using Transwells (6.5 mm diameter; 8 μm pore size polycarbonate membrane) obtained from Corning. 6 Cells (1 × 105) in 0.5 ml serum-free medium were placed in the upper chamber, whereas the lower chamber was loaded with 0.8 ml medium containing 10% FBS. The total number of cells that migrated into the lower chamber was counted after 24 h of incubation at 37°C with 5% CO2.
Cell Invasion Assay.
This was carried out essentially as described (8 , 9) . Transwells (BD Biocoat Matrigel 24-well invasion chamber) with filters coated with extracellular matrix (ECMatrix gel) on the upper surface were obtained from BD Biosciences. 7 The experiments were performed according to the manufacturer's protocol. Cells (1.84 × 105) were added to the upper chamber in serum-free medium containing 0.1% BSA, and the total invasive cells in the lower chamber were counted after 48 h of incubation at 37°C with 5% CO2.
Confocal Microscopy.
The pools of cells stably expressing EGFP-PRLs were seeded onto glass coverslips and grew for 24 h. Cells were washed twice with PBSCM and then fixed in 3% paraformaldehyde for 20 min at room temperature. After three more washes with PBSCM, the cells were permeabilized for 15 min with 0.1% saponin in the same buffer. Cells were washed three times with PBSCM and incubated with TRITC-conjugated Phalloidin (Molecular Probes) for 1 h. The cells were washed four times with PBSCM and mounted onto a glass slide with one drop of antifade reagent in PBS glycerol (Biomedia Corp., Foster City, CA), and kept at 4°C in the dark until analysis. Confocal imaging was performed using a laser scanning head (MRC 1024; Bio-Rad Laboratories, Hertfordshire, United Kingdom).
Live Cell Imaging.
Cells (5 × 105) were seeded onto each 35-mm glass bottom dish (MatTek Co., Ashland, MA) and cultured at 37°C with 5% CO2 for 24 h. The confluent monolayer of cells was wounded as described in the legend to Fig. 2 ⇓ , and the culture medium was then replaced with HEPES-buffered serum-free medium. Cell movement was monitored with a Nikon inverted microscope using a ×40 oil lens. The microscope was equipped with a homemade temperature control chamber set at 37°C. Time-lapse series of images were captured using a Bio-Rad Radiance 2000 confocal system and analyzed using LaserPix software (Bio-Rad).
Metastasis Assay.
Ten-week old female nude mice (Jackson Labs) were each injected via the tail vein with PRL-1-, -3-, or β-gal-expressing cells (5 × 105). Mice were sacrificed 25 days after the tail vein injection, and all of the tissues were examined for metastasis. Tissues with metastases were either photographed for gross morphology or analyzed by H&E staining on fresh-frozen sections.
Myc-PRL-3 and Myc-PRL-1 Enhance Cell Motility and Invasive Activity.
We have previously established stable CHO cell lines expressing Myc-tagged PRL-1 (Myc-PRL-1, clone 9), Myc-tagged PRL-3 (Myc-PRL-3, clone 36), and as a negative control, β-gal (β-gal, clones 8 and 13). The β-gal-expressing CHO cells have no detectable endogenous PRL expression (Fig. 1, A and B) ⇓ . Heterologous protein expression was achieved by transfection of the cells with the tetracycline-responsive pStar vector containing the appropriate cDNA (3 , 7) . Tetracycline-enhanced overexpression of Myc-PRL-1 or -3 was toxic to the cells, but a significant level of Myc-PRL-1 or -3 was constitutively expressed in these stable cell lines without tetracycline induction (Fig. 1C) ⇓ . Thus, we focused on determining the effect of the constitutive levels of Myc-PRL-1 and -3 expression on cell migration and invasion. When cell motility was examined using the standard Transwell assay (8) , ∼5-fold more Myc-PRL-1- and -3-expressing cells migrated to the bottom chamber than did the control β-gal-expressing cells (Fig. 2A) ⇓ . The invasive capacities of the cells were assessed using Transwells with filters coated with extracellular matrix gel (8 , 9) , and were found to be enhanced ∼8-fold by Myc-PRL-1 or -3 expression compared with control β-gal-expression (Fig. 2B) ⇓ . To confirm these observations, cell motility was independently assessed by wound-healing assays (10) . As shown in Fig. 3A ⇓ , Myc-PRL-1- and -3-expressing cells migrated much faster than β-gal-expressing cells. The Myc-PRL-expressing cells migrated a ∼5-cell distance, whereas the β-gal control cells migrated only ∼1-cell distance during a 5-h incubation after wounding. Thus, Myc-PRL-3 and -1 cells have greatly enhanced motility and invasive ability as compared with control cells. To more exactly measure the velocity of cell motility for these cell lines, live cell time-lapse imaging analysis was performed on Myc-PRL-3 cells (Supplementary Data, video 1) and control β-gal cells (Supplementary Data, video 2) for 400 min. Images obtained at the start and end of this time period are shown in Fig. 3B ⇓ . The front line of the Myc-PRL-3 cell sheet at the wound edge moved much faster than did that of control β-gal cells. The movement of individual cells at the leading edge was tracked manually using LaserPix software (Bio-Rad). The velocities of Myc-PRL-3-expressing cells and control β-gal-expressing cells were calculated, respectively, as 14.70 ± 4.41 μm/h and 5.04 ± 2.08 μm/h (P < 0.00001, by Student's t test; Fig. 3C ⇓ ).
PRL expression in CHO-Myc-PRL-1, CHO-Myc-PRL-3, and CHO-β-gal stable cell lines. A, lysates (25 μg) from the stable cell lines indicated at the top of the figure were analyzed for PRL expression by immunoblotting with anti-PRL-1 rabbit serum and were also probed with antibody to the Cdk inhibitor p27Kip1 as a loading control. The anti-PRL-1 serum cross-reacts with PRL-3 (Lane 3). B, the lysates were independently probed with anti-PRL-3 rabbit serum, which cross-reacts with PRL-1 (Lane 2). C, cell lysates were probed with mouse anti-Myc antibody 9E10 to detect Myc-tagged PRL-1 and -3, and also probed with anti-RhoGDI1 rabbit antibody as a loading control. Endogenous PRLs were not detected in the β-gal clone 13 (Lane 1) or clone 8 (data not shown).
Myc-PRL-1 or Myc-PRL-3 expression enhances cell motility and invasiveness. A, the migration of stable CHO cell lines expressing β-gal, Myc-PRL-1, or Myc-PRL-3 was quantitated over 24 h using the Transwell assay. B, the invasive abilities of the same cell lines were examined over 48 h using Transwells with filters coated with extracellular matrix. In both A and B, the bars represent the mean of three independent experiments; bars, ±SD. Three separate Transwells were used in each independent experiment for each cell line.
Enhanced motility of Myc-PRL-1- and Myc-PRL-3-expressing cells in wound-healing assays. A, monolayers of confluent Myc-PRL-1-, Myc-PRL-3-, and β-gal-expressing cells were wounded with yellow pipette tips. After washing with warm PBS, the cells were incubated in fresh culture medium. The wounded areas were photographed at the beginning (0 h, top panels) and the end (5 h, bottom panels) of the assay. Bar, 100 μm. B. images are shown from the start (0 min, a and c) and end (400 min, b and d) of the time-lapse videos of Myc-PRL-3-expressing cells (a and b, from video 1) and β-gal-expressing cells (c and d, from video 2) monitored in wound-healing assays. The black lines demarcate the original wound edge. Tracked cells are labeled as T1 to T9. The migration paths taken by these cells are indicated with yellow lines. Bars, 50 μm. C, the velocities of migration of the tracked cells are shown. Brackets indicate the number of cells used for velocity measurements.
EGFP-PRL-3 Is Enriched at Plasma Membrane Structures Involved in Cell Movement.
Our previous study has shown that Myc-PRL-1, -2, and -3 are distributed to the plasma membrane and endosomal compartments (3) . In view of the enhanced migration and invasion properties of PRL-1- and -3-expressing cells, we more closely examined the localization of PRL-3 on the cell surface using a pool of stable-transfected CHO cells expressing EGFP-tagged PRL-3. The EGFP-PRL-3 was enriched in several membrane processes on the cell surface, including ruffles, protrusions, and some vacuolar-like membrane extensions (Fig. 4) ⇓ . These structures have been demonstrated to play a role in cell movement (11) .
EGFP-PRL-3 associates with various membrane structures involved in cell movement. CHO cells expressing EGFP-PRL-3 were processed for fluorescence microscopy without (a–c) or with labeling with TRITC-conjugated phalloidin (d–f). EGFP-PRL-3 was enriched in membrane processes such as ruffles (a, d, and e, long arrows), membrane protrusions (a and b, short arrows), and vacuolar-like and semivacuolar-like membrane extensions (c, arrowheads). These cells were also processed for labeling with TRITC-conjugated phalloidin (Molecular Probes) to detect the distribution of endogenous actin filaments. EGFP-PRL-3 (d), phalloidin-labeled actin (e), and the merged image (f) are shown. Bar, 10 μm.
Catalytically Inactive PRL-3 Has Significantly Reduced Migration-promoting Activity.
To probe the molecular basis for PRL-3-enhanced cell motility and invasion, we next examined whether its catalytic activity as a phosphatase is coupled to an ability to promote cell migration. We created a mutant form of EGPF-PRL-3, EGPF-PRL-3 (C104S), by mutating the essential Cys104 of the phosphatase active site signature motif to Ser. This active site cysteine is invariant among all of the PTPs (12) , and without exception and including PRL-3 (13) , mutation of this residue to serine or alanine abolishes PTP activity (12 , 14) . A pool of stably transfected CHO cells expressing EGFP-PRL-3 (C104S) was established. Using live cell imaging, the intracellular distribution of EGFP-PRL-3 and EGPF-PRL-3 (C104S), and the migration behavior of the cells were studied during 4-h wound-healing assays. The first and the last images of the series are shown in Fig. 5, A ⇓ (fluorescence) and B (phase contrast). Examination of EGFP-PRL-3 localization showed that it was concentrated at membrane structures (ruffles and protrusions; Fig. 5A ⇓ ) that were dynamic during cell movement (Supplementary Data, video 3). The EGFP-PRL-3 (C104S) was similarly associated with membrane structures (Fig. 5A ⇓ ; Supplementary Data, video 4); however, reduced motility was noted in EGFP-PRL3 (C104S) -expressing cells as compared with EGFP-PRL-3-expressing cells. By tracking individual cells, the average calculated velocities for EGFP-PRL-3 and EGFP-PRL-3 (C104S) cells were 24.01 ± 3.55 μm/h and 12.86 ± 4.99 μm/h, respectively (P < 0.0005, Student's t test; Fig. 5C ⇓ ).
PRL-3 motility-enhancing activity is coupled to its phosphatase active site. Equivalent numbers (5 × 105) of EGFP-PRL-3 and EGFP-PRL-3 (C104S) -expressing stable cell pools were seeded, wounded 24 h later, and then video-recorded at 1 frame per 1 min over a period of 4 h. A, images of pooled cells stably expressing EGFP-PRL-3 from the start (0 min) and end (240 min) of time lapse video 3 are shown in a and b. Images of pooled cells stably expressing inactive mutant EGFP-PRL-3 (C104S) from the start (0 min) and end (240 min) of time-lapse video 4 are shown in c and d. Note that only cells strongly expressing EGFP-PRL or its mutant form are apparent in these images. Both EGFP-PRL-3 and EGFP-PRL-3 (C104S) are associated with various membrane processes during cell migration. However, EGFP-PRL-3 is associated with more dynamic plasma membrane structures during cell migration (videos 3 and 4). Tracked cells are represented as T1 to T10. The migration paths taken by these cells are indicated with red lines. Bar, 20 μm. B, phase contrast images show equivalent densities of the plated EGFP-PRL-3 and EGFP-PRL-3 (C104S) -expressing cell pools. The black lines demarcate the edges of the cell monolayer at 0 and 240 min. Bar, 50 μm. C, the velocities of migration of the tracked cells are shown for both stable pools. Brackets indicate the number of cells used for velocity measurements.
PRL-3- and PRL-1-expressing Cells, But Not Control Cells, Induce Metastatic Tumor Formation in Nude Mice.
Key events in metastasis include the ability of tumor cells to survive in and then leave the circulation to extravagate into a tissue, begin and maintain growth in this tissue to form preangiogenic micrometastases, and then develop a blood supply that enables formation of macroscopic tumors (15, 16, 17) . We used an experimental metastasis assay to examine the abilities of the PRL-expressing cells to carry out this process in vivo. Five × 105 cells from Myc-PRL-1, -3, and control β-gal clones were respectively injected into the tail veins of 10-week-old female nude mice, thus introducing these cells directly into the circulatory blood system of the animals. One of the mice carrying PRL-3-expressing cells died on day 25 after injection and was found to have had an extensive lung metastasis. At this time, examination of all of the tissues in these mice revealed that 10 of 10 mice injected with PRL-1-expressing cells had lung tumors (Fig. 6) ⇓ . Ten of 10 mice carrying PRL-3-expressing cells had more extensive lung tumors; in addition, 2 of them had liver metastasis tumors (Fig. 6) ⇓ . In contrast, no tumors were found in 10 mice injected with β-gal-expressing cells (Fig. 6) ⇓ . Human colon cancer cells are taken by the hepatic-portal circulatory system first to the liver, the primary site of metastasis. In our experimental metastasis assay, cells injected into the tail vein initially arrive in the lung.
The expression of Myc-PRL-1 and Myc-PRL-3 promotes cell metastasis in vivo. Mice were examined 25 days after tail vein injection of (5 × 105) Myc-PRL-1-, Myc-PRL-3-, or β-gal-expressing (clone13) cells. Metastatic tumors were found in the lungs of all of the mice injected with Myc-PRL-1 and Myc-PRL-3 cells, whereas 2 of the 10 mice injected with Myc-PRL-3 cells also developed liver metastases. The top panels show the gross morphology of the respective lungs and/or liver, whereas the bottom panels show the histological morphologies of sections derived from the respective tissues and stained with H&E. T stands for areas with tumor. Bars, 2.5 mm and 100 μm for the top and bottom panels, respectively.
Tumor cell metastasis is responsible for most cancer deaths (15, 16, 17) . However, the specific molecular changes in a tumor cell that promote the metastatic process are largely unclear. The recent discovery that PRL-3 is the only gene consistently overexpressed in 100% of 18 colorectal cancer liver metastases examined suggests that PRL-3 is a key factor involved in metastasis of this and possibly other types of tumors (6) . This striking report prompted us to directly test whether stable expression of the protein tyrosine phosphatase PRL-3 and its relative PRL-1 in nonmetastatic CHO cells could induce the acquisition of metastasis-associated properties such as enhanced invasiveness and motility, and confer metastatic ability. Greatly enhanced motilities of Myc-PRL-3- and -1-expressing cells were indeed observed using two independent assays. Firstly, quantitative Transwell-based assays showed that PRL-3 and -1 expression enhanced cell migration by ∼5-fold. Secondly, qualitative wound-healing assays and live-cell imaging studies supported this conclusion. Furthermore, the velocity of migration of Myc-PRL-3 expressing cells was ∼3-fold higher than the β-gal-expressing control cells. In addition to increased motility, the Myc-PRL-3- and Myc-PRL-1-expressing cells exhibited an ∼8-fold increase in invasive abilities over the control β-gal-expressing CHO cells as assessed by an ECMatrix gel-membrane coated Transwell assay. As CHO cells have no detectable endogenous PRL expression (Fig. 1) ⇓ , the crucial event that induced the gain of motile and invasive properties by the CHO cells was the expression of PRL-1 or -3.
This conclusion was additionally strengthened and extended by our studies of independently and differently generated CHO cells stably expressing wild-type active PRL-3 or a catalytically inactive PRL-3 (C104S) mutant. In this system, PRL-3 was tagged with EGFP instead of Myc and expressed using the pEGFP-C1 instead of the pSTAR plasmid vector. Also, pools of these stably transfected cells were used for analysis to avoid clonal variations. Notably, EGFP-PRL-3 expression dramatically enhanced cell migration, whereas mutant EGFP-PRL-3 (C104S) expression had a greatly reduced effect on promoting cell migration, suggesting that the phosphatase activity of PRL-3 is required for optimal PRL-3-dependent migration. The CHO-EGFP-PRL-3 cell pool exhibited a higher velocity of migration than the CHO-Myc-PRL-3 cell line (24.01 ± 3.55 versus 14.70 ± 4.41 μm/h, respectively). This could be because of an inherent effect of EGFP on the cells. Alternatively, it may be because of different PRL-3 expression levels driven by different promoters (cytomegalovirus promoter of pEGFP-C1 versus the uninduced tetracycline-inducible operator mini promoter of pSTAR) or gene copy numbers. In support of this, we observed that cells with different EGFP-PRL-3 expression levels showed different morphology and motility: the higher the expression levels, the faster the cells moved. Also, the PRL-3 gene was found in multiple copies within a small amplicon in 25% of actual colorectal cancer liver metastases (6) , indicating that gene amplification is an important mechanism to effect elevated PRL-3 expression in metastases. Interestingly, our independent experiments revealed a difference in migration velocity between the CHO-EGFP-PRL-3 (C104S) cell pool and the control CHO-β-gal cell line (12.86 ± 4.99 versus 5.04 ± 2.08 μm/h, respectively), either because of an inherent EGFP effect or raising the possibility that high levels of EGFP-PRL-3 (C104S) expression could enhance cell motility in a phosphatase-independent manner. Nevertheless, both Myc-PRL-3- and EGFP-PRL-3-expressing cells demonstrated enhanced cell motility compared with their respective β-gal- or EGFP-PRL-3- (C104S) expressing control cells by 3-fold and 2-fold.
The rapid metastasis in our animal study suggests that PRL-3 and -1 can act as key players to initiate and maintain tumor cell growth in a "foreign territory." Examination of the expression levels of PRL-1, -2, and -3 in other metastatic tumors will provide insight regarding the possibility that these PRL-PTPs, and perhaps PRL-2, could be associated in general with metastatic events. The discovery that PRL-3 is overexpressed in all of the examined metastatic colorectal cancers (6) indicates that PRL-3 is a potential molecular marker for clinical estimation of tumor aggressiveness. This, together with our present study demonstrating that PRL-3 overexpression promotes cellular properties associated with metastasis as well as the end point formation of macroscopic tumors in an in vivo metastasis assay, suggest that PRL-3 may additionally present an excellent target for intervention with colorectal tumor metastasis. This could be an important new therapeutic opportunity, because most of the described genetic alterations in colorectal cancers involve the inactivation of tumor suppressor genes, which are difficult to target with drugs (18 , 19) . Because optimal PRL-3-enhanced cell migration is dependent on preservation of its catalytic function, the consensus phosphatase motif will potentially be a therapeutic target. Also, the prenylation-dependent association of the PRL-PTPs with cell membranes (3) , coupled with the present description of the association of PRL-3 with membrane structures including ruffles, protrusions, and some vacuolar-like membrane extensions could represent another opportunity for intervention. These membrane structures have been demonstrated to play a role in cell movement and invasion (10 , 11) . The expression of membrane-associated PRL-3 may induce dephosphorylation of target substrates at the cell membrane to modulate the organization of the plasma membrane in such a way to promote cell motility and invasion. No protein substrate has yet been identified for PRL-3, and classic in vitro phosphatase substrates are poorly dephosphorylated by recombinant PRL-3. 8 This latter property is similar to PTEN, a 3′-lipid phosphatase of phosphoinositides (20) . Because PRL-1, -2, and -3 share some homology with PTEN in the phosphatase active site "signature motif" and flanking regions (1) , one might speculate that PRL-3, like PTEN, acts as a lipid phosphatase at the cytoplasmic face of the plasma membrane. Our data raise the possibility that disruption of this prenylation-dependent association could effectively block the function(s) of PRL-3 in cell migration and invasion. In this regard, we have shown previously that treatment of cells with the prenyltransferase inhibitor FTI-277 (which specifically inhibits protein farnesylation; Ref. 21 ) induces the subcellular redistribution of PRLs from plasma membrane to the nucleus (3) . Notably, some reports support the potential use of farnesyltransferase inhibitors as antimetastatic agents in cancer therapy (22 , 23) . It will be of considerable interest to determine whether such inhibitors of prenylation affect PRL-3- or PRL-1-dependent cell migration, invasiveness, and metastasis in our cell model system. In conclusion, the viability of PRL-3 as an antimetastatic target warrants assessment.
We thank Drs. Bor Luen Tang, Xin-Min Cao, and Xiao Hang Yang for critical reading of the manuscript, and Drs. Yin Hwee Tan and Louis Lim for their support.
↵1 Supported by research grants from The Agency of Science, Technology and Research (A∗ STAR), Singapore.
↵2 Supplementary data for this article are available at Cancer Research Online (http://cancerres.aacrjournals.org).
↵3 To whom requests for reprints should be addressed, at Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Singapore. Phone: 65-6874-3752; Fax: 65-6779-1117; E-mail: mcbzengq{at}imcb.nus.edu.sg
↵4 The abbreviations used are: PRL, phosphatase of regenerating liver; CHO, Chinese hamster ovary; PTP, protein-tyrosine phosphatase; β-gal, β-galactosidase; FBS, fetal bovine serum; EGFP, enhanced green fluorescent protein; PBSCM, PBS with CaCl2 and MgCl2; TRITC, tetramethylrhodamine isothiocyanate; PTEN, phosphatase and tensin homolog deleted in chromosome 10.
↵5 Internet address: http://www.clontech.com/index.shtml.
↵6 Internet address: http://www.corning.com.
↵7 Internet address: http://www.bdbiosciences.com/index.shtml.
↵8 Unpublished observations.
Received January 6, 2003.
Accepted March 20, 2003.
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Vogelstein B., Lane D., Levine A. J. Surfing the p53 network. Nature (Lond.), 408: 307-310, 2000.
Maehama T., Taylor G. S., Dixon J. E. PTEN and myotubularin: novel phosphoinositide phosphatases. Annu. Rev. Biochem., 70: 247-279, 2001.
Lerner E. C., Qian Y., Blaskovich M. A., Fossum R. D., Vogt A., Sun J., Cox A. D., Der C. J., Hamilton A. D., Sebti S. M. Ras CAAX peptidomimetic FTI-277 selectively blocks oncogenic Ras signaling by inducing cytoplasmic accumulation of inactive Ras-Raf complexes. J. Biol. Chem., 270: 26802-26806, 1995.
Ohkanda J., Knowles D. B., Blaskovich M. A., Sebti S. M., Hamilton A. D. Inhibitors of protein farnesyltransferase as novel anticancer agents. Curr. Top. Med. Chem., 2: 303-323, 2002.
Andela V. B., Rosenblatt J. D., Schwarz E. M., Puzas E. J., O'Keefe R. J., Rosier R. N. Synergism of aminobisphosphonates and farnesyl transferase inhibitors on tumor metastasis. Clin. Orthop., 397: 228-239, 2002.
You are going to email the following PRL-3 and PRL-1 Promote Cell Migration, Invasion, and Metastasis
Cancer Res June 1 2003 (63) (11) 2716-2722;
Activating Mutations of the Noonan Syndrome-Associated SHP2/PTPN11 Gene in Human Solid Tumors and Adult Acute Myelogenous Leukemia
Recombinant Listeria Vaccines Containing PEST Sequences Are Potent Immune Adjuvants for the Tumor-Associated Antigen Human Papillomavirus-16 E7
2-Arachidonoylglycerol
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text:
Gabriel Michael Bateman (Turlock, Kalifornia, 2004. szeptember 10. –) amerikai színész. Leginkább a számos horrorfilmben játszott alakításairól ismert, ilyen például az Annabelle (2014), az Amikor kialszik a fény (2016), a Gyerekjáték (2019) és a Téboly (2020).

Élete és karriere

Bateman a kaliforniai Turlockban nevelkedett, de Kaliforniában délre költözött, amikor színészi pályafutását elkezdte. Van egy nővére, Talitha Bateman, aki szintén színész.

Filmográfia

Filmek

Televíziós sorozatok

Videóklippek

Jegyzetek

További információk

Amerikai színészek
2004-ben született személyek
Élő személyek
Kaliforniaiak

redpajama_set_name: RedPajamaWikipedia


- When setting an avatar, it does display in the settings page, but not in the profile page. Gravatar avatars do work and overwrite previously selected avatars.
- When trying to move a forum thread (wanted to move this to Support), It says the thread is moved, but it remains solidly in place.
Other than that I will continue my quest to squash bugs. I'll list them here.
Funnily enough, when trying to move it from inside the topic, it didn't work. When adminning it from the forum page, rather than inside the topic, it did.
I did notice that the post a reply button at the bottom didn't work. The one at the top did.
Thanks for those. The CMS comes with its own avatar system, but our designers decided to go with Gravatar for our specific install so I think it's clearly confusing the system. Will flag that one up with the designers to sort out.
Will double-check on the forum issue, the forums are still very much a WIP, so we might have to try this again when the we're actually using the appropriate skin.


text:
An Overview of Extracellular and intracellular small-molecule AKT inhibitors
Furthermore, the normal osteoblast cell line hFOB1
Furthermore, the normal osteoblast cell line hFOB1.19 was employed to explore whether salidroside induced osteoblast apoptosis with the same concentrations. reported that salidroside has anti-fatigue, anti-aging, anti-oxidant, anti-inflammatory, neuroprotective and cardiovascular protective effects (9-12). A literature review revealed that salidroside exhibits antitumor effects in various tumors, including fibrosarcoma (13), bladder carcinoma (14), lung carcinoma (15), breast carcinoma (16) and renal cell carcinoma (17) and the underlying molecular mechanism. Materials and methods Cell culture and treatment Human osteosarcoma cell lines MG63 and U2OS (ZQXZBIO, Shanghai, China), were selected to assess the antitumor effects of salidroside. Cells were cultured in Dulbecco's modified Eagle's medium combined with high-glucose medium (DMEM-HG) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (all Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA), and were maintained in a 37C humidified incubator with 5% CO2. Cells were harvested with a 0.25% trypsin-0.02% EDTA solution and passaged when the cells attained ~80% confluence. Salidroside (Fig. 1A; purity 99%, MedChem Express, Monmouth Junction, NJ, USA) was dissolved in PBS at room temperature and filtered through a 0.22-(24) reported that salidroside combined with antitumor agents exerted excellent antitumor effects in colorectal cancer. Qi (25) revealed that salidroside had a direct inhibitory effect on the proliferation, migration and invasion of gastric cancer cells. In the TMB present study, we first assessed the antitumor effects of salidroside in the treatment of osteosarcoma. We demonstrated that salidroside induced the growth and invasion TMB of osteosarcoma cells, which indicated its therapeutic potential. The pharmacological mechanism of salidroside may be related to the JAK2/STAT3 signaling pathway (Fig. 7). Open in a separate window Figure 7 Diagram of the mechanism of salidroside-induced apoptosis, cell cycle arrest and suppressed invasion of osteosarcoma cells via inhibition of the JAK2/STAT3 signaling pathway. Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; JAK, Janus kinase; MMP, matrix metalloproteinase; STAT3, signal transducer and activator of transcription 3. HDAC-A Cell proliferation is an important marker for tumor development. Therefore, inhibiting tumor growth (by promoting tumor cell apoptosis) is the most important objective in preventing tumor progression (26). The MTT assay is widely used in bioactive factor activity assays, large-scale antitumor drug screening and cytotoxicity assays (27). In the present study, the results of the MTT assay revealed that salidroside significantly inhibited the viability of osteosarcoma cells in a time- and concentration-dependent manner. The results of cell morphological observations and flow cytometric apoptosis detection further indicated that the decrease in cell viability induced by salidroside was associated with cell apoptosis. We investigated whether the expression of apoptotic-related proteins via western blot analysis, and the expression of the Bcl-2 and caspase families, critical apoptosis-related proteins, were regulated by salidroside. The Bcl-2 and caspase families are specific regulatory proteins of the mitochondrial apoptosis pathway, which is one of the main pathways of apoptosis (28). Our results indicated that TMB the mitochondrial apoptosis pathway is involved in salidroside-mediated apoptosis of osteosarcoma cells. In addition, dysregulated cell cycle distribution is another feature of tumor development, and the induction of cell apoptosis is accompanied with cell cycle arrest (29). Flow cytometric cell cycle analysis is widely used for evaluating changes in cell cycle distribution (30). We reported that salidroside triggered G0/G1 phase arrest in osteosarcoma cells, which was consistent with previous reports (16,31). Then, the present study investigated the expression of cell cycle-related proteins using western blot analysis; the expression of cyclin D1 and p21 were revealed to be regulated TMB by salidroside. Therefore, we concluded that salidroside induced the apoptosis of osteosarcoma cells by inducing G0/G1 phase arrest. We suggested that salidroside may function as an agonist to induce the apoptosis and G0/G1 phase arrest of osteosarcoma cells, and may represent as an alternative therapeutic strategy for the treatment of osteosarcoma. Invasion, which generally leads to metastasis, can be used to predict tumor malignancy (32). Previous research reported that early metastasis (particularly pulmonary metastasis) remains as the main cause of mortality in ~40% of patients with osteosarcoma (33). The results of Transwell assays demonstrated that salidroside significantly.
Posted inPim-1
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(D) The 20 most frequently mutated genes in 262 CRC tumors are shown
Nat
The targets grid map was set and calculated to 606060 points with grid spacing of 0
1H-NMR (DMSO-d6) (ppm) 12
This enzyme degrades beta lactam-based antibiotics to any therapeutic effect prior

redpajama_set_name: RedPajamaCommonCrawl


While many of us trust the advice of so-called "experts" when it comes to our financial well-being, the fact is that many of these professionals either aren't objective enough or simply have an opinion that they want to push on us. This is ironic in that finance is a very subjective discipline, and many financial issues can be resolved by combining a few simple rules with a solid understanding of your own personal preferences.
I know how difficult it can be to overcome these obstacles, but I also believe that through well-rounded discussions of important financial topics, you can enhance your understanding of this field and improve your performance within it. That's why, in 2005, I started my blog as a forum for discussing commonly asked financial questions. Within months, the blog became a widely syndicated resource for both ordinary investors and industry professionals.
Now, with this book, I will show you how to navigate the many informational outlets available online and provide you with the tools you'll need to get the most out of them. Divided into four comprehensive parts—First Perspectives on Money, Building Wealth Requires Planning, Living in a Financial World, and Thinking Long-Term about Money Management—this one-of-a-kind guide may change the way you live your financial life.
Starting at the beginning of a typical personal financial journey, the book answers basic questions such as how to choose a bank and how to read a credit score. It then moves on to address the essential savings and investment issues that many of us will face during our working years—from figuring out IRAs and 401(k)s to picking stocks and mutual funds. Navigating the Financial Blogosphere concludes with an accessible analysis of long-term financial topics, such as retirement and Social Security, and a straightforward explanation of how to manage your portfolio through asset allocation and rebalancing strategies.
Composed of concise, yet comprehensive chapters that build in complexity as the book progresses, Navigating the Financial Blogosphere will help you connect with some of the best financial information possible and show you how to use it to boost your chances at success in today's ever-changing financial world.
Thanks and best of luck to all!
Please find a table of contents below.
Introduction. Why Navigate the Blogosphere?
Part One. First Perspectives on Money.
Chapter 1. How Do I Choose a Bank? Does it Really Matter?
Chapter 2. What Are Personal Financial Statements, and Why Do I Need Them?
Chapter 3. Can a Budget Really Help Me?
Chapter 4. Are Credit Cards My Enemy?
Chapter 5. Are You Scared of Your Credit Score?
Part Two. Building Wealth Requires Planning.
Chapter 6. What Is the Role of a Financial Advisor?
Chapter 7. How Does the Financial Planning Process Work?
Chapter 8. Why is Money Emotional?
Chapter 9. Why Should I Open an IRA?
Chapter 10. What's a 401k Rollover? Is that Something I Can Teach my Dog?
Chapter 11. What's the Difference between a 401k and 403b Plan?
Chapter 12. How do I Pay for College? (Part I). Borrowing Money for your Future.
Chapter 13. How do I Pay for College? (Part II). Saving in Advance.
Part Three. Living in a Financial World.
Chapter 14. Are You Ready to Pick Your Own Stocks?
Chapter 15. What are Alternative Investments?
Chapter 16. How Do I Pick Mutual Funds?
Chapter 17. Exchange-Traded Funds. What are They and Should I Buy One?
Chapter 18. Can I Dabble in Real Estate Too?
Chapter 19. How Can I Unravel the Mortgage Mystery?
Chapter 20. What Are All These Different Financial Designations?
Chapter 21. Exploring the Variable Annuity. What's all the Chatter About?
Part Four. Thinking Long-Term About Money Management.
Chapter 22. How Can I Manage My Portfolio Through Asset Allocation and Rebalancing?
Chapter 23. The Fed? Interest Rates? Inflation? Why Should I Care?
Chapter 24. Life Insurance is Boring. What do I need to Know?
Chapter 25. What Do I Need to Know About Retirement?
Chapter 26. What are my Chances of Getting Social Security?
Chapter 27. What is This Pension Crisis I Keep Hearing About, and How Will It Affect My Retirement?
Securities offered through First Allied Securities, Inc., a registered broker/dealer. Member FINRA / SIPC. Advisery services offered through: Premier Financial Advisors is a NY Registered Investment Advisor. Form ADV part II is available upon request.


Disney's Zootopia preached a message of inclusivity, tolerance and friendship, making millions and millions of dollars in the process. Behind the scenes, however, things are not so amiable. Gary Goldman, writer of Total Recall and producer of Minority Report, has slapped the company with a lawsuit alleging that the movie was his idea.
According to The Hollywood Reporter, Goldman filed suit against Disney today through his Esplanade Productions. The complaint alleges that Disney has stolen ideas for films in the past, including The Lion King, Toy Story, Monsters Inc., Up, Inside Out and other properties.
"They did it with Zootopia, too, when they copied Gary L. Goldman's Zootopia," the complaint adds. "Twice – in 2000 and 2009 – Goldman, on behalf of Esplanade, pitched Defendants his Zootopia franchise, which included a live-action component called Looney and an animated component called Zootopia.
"He provided a treatment, a synopsis, character descriptions, character illustrations, and other materials. He even provided a title for the franchise: 'Zootopia.' Instead of lawfully acquiring Goldman's work, Defendants said they were not interested in producing it and sent him on his way. Thereafter, consistent with their culture of unauthorized copying, Defendants copied Goldman's work. They copied Goldman's themes, settings, plot, characters, and dialogue – some virtually verbatim."
The complaint includes a potentially damning quote from Zootopia director Byron Howard, who once said, "Don't worry if you feel like you're copying something, because if it comes through you, it's going to filter through you and you're going to bring your own unique perspective to it."
Since Zootopia has made over a billion dollars worldwide, there's a lot of potential damages on the table. Goldman is demanding that "Defendants be ordered to pay to Esplanade all damages, including future damages, that Esplanade has sustained, or will sustain, as a consequence of the acts complained of herein, and that Esplanade be awarded any profits derived by Defendants as a result of said acts, or as determined by said accounting."
In a statement, a Disney spokesperson denied the claims. "Mr. Goldman's lawsuit is riddled with patently false allegations," they said. "It is an unprincipled attempt to lay claim to a successful film he didn't create, and we will vigorously defend against it in court."


Ananda Yoga Therapy Training among the first ever accredited programs by IAYT.
Since 2014, Ananda YogaTM Therapy Training, a program of the Ananda School of Yoga & Meditation, has been accredited by the International Association of Yoga Therapists (IAYT). We were one of the first 12 schools to be accredited by IAYT.
IAYT is helping to bring yoga therapy into the Integrative Health model and the prevalent medical model, empowering health care providers to refer patients directly to professionally trained and certified yoga therapists. IAYT champions yoga as a healing art and science. IAYT supports research and education in yoga, and also serves as a professional organization for yoga therapists worldwide.
When someone has completed the minimum of 800 hours of training in an IAYT accredited Yoga Therapy program, they can then apply to become a C-IAYT, which means that they are an IAYT Certified Yoga Therapist.
If you have any calling or interest in pursuing Ananda Yoga Therapy Training, please click here to contact our Program Director. We'd love to have you join us in learning how to take the powerful therapeutic teachings of Ananda Yoga out to serving the world–helping people to improve their health and well-being through the application of yoga philosophy and practice.


Christmas Craft Fair– if you live near Letchworth and need ideas for Christmas presents then why not visit the Letchworth Christmas Craft Fair, it's one of the biggest and brightest craft fairs in the area. There will be work displayed by local and national artists open from Monday to Friday 10.00 am until 17.00 and Saturday from 10.00 until 15.00, until the 20 December, held at the Letchworth Arts Centre, 2 The Arcade, Letchworth Garden City, and best of all admission is free.
Real Ice Skating - Frosts Garden Centre in Willington are celebrating Christmas this year by offering a real outdoor ice-rink until the 8th of January 2013, there is a viewing area, food, treats and stalls. Prices are £8.49 for adults and £5.99 for children, for more information call 01908 583511.
Reindeer Event – the Reindeer are visiting Burwash Manor in Cambridgeshire this weekend from 2.30pm until 7.30 pm, Prices are £7 per adult, £3 per child (a child is 12 and under) with under 3's getting in for free. The Reindeer who have come all the way from Scandinavia, The ticket price includes time with the reindeer (including special photo opportunities) and a visit to see Father Christmas in his Grotto, take a (miniature) steam train to his Grotto. Also on site will be food stalls, mulled wine, live music, and fairground rides, for more info. go to their website @ http://www.burwashmanor.com/02_news_reindeer.asp but hurry as there are only a few time slots available.
The Cambridge Ice Skating rink is back – between the 19th December and the 7th of January, on Parker's Peace Cambridge. There will be a 6,500 sq ft rink, plus a decked area for spectators and a café-bar where you can get some drinks. There will also be great musical entertainment.
A very special way to meet Father Christmas - trains run approximately every 20 minutes from 11am to 3.45pm, cost £6.00 per person. Under 2's Free.
The Cambridge Arts Theatre are showing Jack and the Beanstalk from Saturday 6th December until Thursday 3rd January starring Matt Crosby as Dame Trott Rollan Bell as Sill Billy, Jonny Weldon as Jill, John Weldon and David Nellist with Emily Tierney as the fairy Godmother and Stephen Beckett as Squire Nastee.
Cambridge Corn Exchange - Roald Dahl's George's Marvellous Medicine - from Thurday the 13thof December until 3rd of January. The award-winning Birmingham Stage Company (Horrible Histories, Why The Whales Came and Danny Champion of the World) is back on tour with a brand new adaptation of one of Roald Dahl's funniest and most exciting stories.
Carol's from King's College – choral service is held at 5.30 from Monday to Saturday, and at 10.30 and 3.30 on Sunday. The doors open 15 mins before each service.


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{{> Restaurant
    name="L&L Hawaiian Barbecue"
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    info="Delicious local and Asian-American fusion cuisine served in big portions at reasonable prices!"
    menu=menu}}
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redpajama_set_name: RedPajamaGithub


text:
Introduction: Play
(Rev. Trevor Maloney)
For good reason, our Zen tradition is known for being a little serious, perhaps even harsh. The kyosaku is meant to help practitioners stay awake—and it does—but it still looks like someone getting hit with a stick. Linji was known for his deafening shout, and Te Shan for his tendency to beat his students with a staff. Our ancestors often remind us to be single-minded in our pursuit of truth. With death as our constant companion, what room is there for play, for goofing around?
You could easily focus on the dignity, majesty, and life-or-death seriousness of our tradition, if you wanted to. But, you would be forgetting Chin Niu, who would call his monks to lunch by beating a pot, laughing, dancing, and singing, "Bodhisattvas! Come and eat!" You would be forgetting the spontaneous laughter that came about at moments of awakening. You would be ignoring the Pang family's teasing each other as they sharpened their understanding together.
While buddhas and bodhisattvas are traditionally depicted with a slight smile, or at least a rather warm expression, Bodhidharma, the first Zen ancestor in China, sits there scowling. He's a scary sight. His beard is scraggly. He wears his robe over his head, which gives me the impression that he's hiding, that he doesn't want to talk to me. He probably wouldn't want to talk to me, in fact. He brought a teaching "not dependent on words and letters." Hui-Ke had to cut off his own hand just to get into his dokusan room. Bodhidharma was so dead serious and intent on practicing zazen, the legend says, that he sliced off his eyelids to keep from dozing off. (Fortunately for us, his eyelids fell to the ground and a tea plant sprung up, so we can just have some tea to stay alert.) Google image-search "Bodhidharma" and you'll see some serious-looking faces. Our way is difficult, he seems to say, demanding everything. Don't slack off.
This image is different, however. Hanging in the AZC zendo, it shows Bodhidharma playing with a chubby little kitty, and smiling. I've never seen such a depiction of the blue-eyed barbarian. Sure, practice hard, it seems to say, but know that this is all for the sake of liberation. Dogen tells us that if you realize that your mind is unlimited, "your priorities about everything change immediately." Maybe playing with the cat, or tying a pretty red ribbon around his neck, becomes the most important thing. Devote yourself wholeheartedly to this way that directly points to the absolute. Know that your mind is unlimited. Smile. Bodhidharma wants to play.
(Visit Trevor's blog at: http://thebigoldoaktree.blogspot.com/)
Remembering Play
(Kim Mosley)
We sat two periods of zazen today, during which time there were a few kids playing in the yard next to the zendo. At first I was annoyed, because I was having my private conversations with myself, and these kids were interrupting me. Then I started to sense it was me, 55 years ago, playing, not them. I moved from the cushion to the outdoors. I realized I was existing in two "time zones" at the same time. Later, after sitting, I saw the kids through the window. They had red shirts on. No, I thought. I wasn't wearing a red shirt that day!
(more of Kim Mosley's art can be found on his blog: mrkimmosleywrite.blogspot.com)
(daigu)
nine bows
for the bodhisattva
the thus come one's
true disciple
who teaches children
by heart.
nine bows for bodhichitta,
freeze tag
and the immutable law
of hop scotch
and the fool's favorite
peek-a-boo.
with closed eyes,
I see you.
play at work, work at play
Sometimes we mistakenly believe that kids play and adults work, but one just needs to watch a toddler and see how dedicated they are to the task at hand to realize they are learning about their universe in record time and will not (or rarely, as in Jasper's case below) be led astray by any distractions.
Adults, on the other hand, with their great understanding of the world, seem to have lots of time for play—sports, movies, and candlelight dinners.
QUESTION: AT WHAT AGE DO HUMANS PLAY? WHY?
(more of Kim Mosley's art can be found on his blog: blog.kimmosley.com)
(Cristina Mauro)
Flung across their row of beds
my three boys fell asleep playing.
One lays diagonal across the mattress
chin propped up and arms outstretched.
A small blue airplane in one hand waits
to fly through the line of light beaming
from the flashlight in his other hand.
His twin brother has his head nested on the pillow
and arms down by his sides
but the covers are rumpled
and the headband circled twice around his upper arm
seems to suggest he has unusual powers.
The youngest sleeps upside down
with a red flip flop clinging to one foot.
His stuffed rabbit is not in the usual place
and his hand hangs over the mattress edge
holding a single sheet of blank paper.
The hour that vanished hangs over them and waits.
2005 Summer Intensive
One hot day during Summer Intensive 2005, four participants (Nancy Webber, Anita Swan, Lila Parrish, Pat Yingst), crazed by the relentless chirping of cicadas and the unceasing watching of their thoughts, unleashed their impulses on the building and grounds of the AZC. Their mayhem did not go un-recorded! See it here.
The Ballad of Bodhidharma
(John Grimes)
Bodhidharma went to China
Just to see what he could see.
When he got there all he said is:
"What will be is what will be.
But delusion mars your vision
Of what is and what has been,
So just sit right down and turn around
And start to practice zen."
"Once you're seated and you're settled
Then you focus on your breath.
In good time it will be obvious
That twixt now and certain death
There's no you, no I, no me, no thee,
He, she, her, it or him.
There's just us, right here, and us, right now.
There is no they or them."
"All appearance is delusion
There is nothing there at all.
But do not activate your mind
When that nothing comes to call.
In the mind, no coughing, sighing
When you hate or when you crave.
There's no gain or loss – don't laugh or cry –
Come join me in my cave!"
Bodhidharma barks and snaps and snarls
And bristles in your face,
Yet he'd love a kiss and cuddle
Any time, most any place.
He's a puzzle, is our Bodhi,
As we struggle to perceive
That the only dharm' of karma is
We get what we receive.
(This can also be sung to the tune of the final rondo of The Elixir of Love by Donizetti)
Showdown at the Genjo Corral
(Peg Syverson)
Austin Zen Center 2003 Summer Intensive
Cast: Dogen, Billy (the kid), Bartender, Wyatt, Shane
Scene: A West Texas saloon, Showdown at the Genjo Corral
The arrival:
[Wyatt, Shane and Billy, seated at the table with a go game between Wyatt and Shane]
[Bartender is wiping off the bar when the robed stranger, Dogen, enters, carrying a set of oryoki bowls and a fan.]
Bartender: What can I do for ye, stranger?
Dogen: Nothing.
Bartender [sets down a shot glass]: Well, what'll it be?
Bartender rolls his eyes and takes away the glass.
Bartender: I guess you're not from around these parts. So, then, what do you think of West Texas, pardner?
Dogen: As my great granddaddy said, "Vast emptiness, nothing holy."
The gamblers:
Wyatt: You got that right, stranger. Say, what's your handle?
Dogen: Dogen
Shane: Why don't you come along, little dogie, and set right here by me.
[Dogen moves to the table and takes a seat next to Billy]
Wyatt: We're just having a friendly game of Go with a little wager on the side. Table stakes, nothing much.
Billy [eagerly]: What are you gonna bet, Dogen?
The teachings:
On self:
Wyatt: Well, then, what have you got to say for yourself?
Dogen: To study the Buddha way is to study the self;
Billy [helpfully]: Well, if you want to know the way to Buda, it's a five day ride due east of here.
Wyatt: Hold on, Billy, let the man say his piece. [turns with interest to Dogen]
Dogen:To study the self is to forget the self.
Shane: well, now, Dogie, would that be the ontological self or the psychological self?
[chuckles]
Dogen: To forget the self is to be actualized by myriad things.
Wyatt: Hell, talkin' about myriad things, I got a lot on my plate too. My to-do list is a mile long. But what the Sam Hill are you talking about?
Dogen: When actualized by myriad things, your body and mind as well as the bodies and minds of others drop away.
Billy [excitedly]: You mean, like a lynching?
Dogen: No trace of realization remains, and this no-trace continues endlessly.
Wyatt: What??? Now you're talking like a crazy man, ain't he, Shane?
Form and emptiness:
Shane: Wait a minute, Wyatt. I think the man's actualizing some fundamental point. Lookee here. See that corral? It's form, and emptiness. Without the fence posts and rails, you got no corral, without the emptiness, you just got you a pile of lumber, and no place to put the herd.
Wyatt (triumphantly) : But supposin' you was to turn them posts and rails into firewood and burn 'em up? Then where's your dadgummed corral, eh?
Dogen: Firewood becomes ash, and it does not become firewood again.
Shane (ruefully): He's got you there, Wyatt.
Shane: Wind's kickin' up again.
Wyatt [shaking the head]: Like always. And it gets into everything. No point in even fanning yourself.
Dogen: Although you understand that the nature of the wind is permanent, you do not understand the meaning of its reaching everywhere.
Wyatt [annoyed]: Goddamned if I don't, and I've got the sand in my ass to prove it.
Billy: What do you mean, Dogen?
Dogen (fans himself with his fan)
Dogen: It's possible to illustrate this with more analogies, with birds, and fish, and boats, and so on... but I won't. The moon is on the dewdrops, farewell, bodhisattvas-mahasattva.
Wyatt [narrowing the eyes]: What did you just call us, son?
Shane: Git along, little Dogie. I'll enlighten this one.
Exit:
[Shane and Wyatt return to their go game, murmuring and shaking their heads, Billy looks on, and the bartender wipes down the bar.]
Wyatt: Another sake, Bartender!
Dogen, leaving [thoughtfully]: When buddhas are truly buddhas they do not necessarily notice that they are buddhas.
Play Full Out
(Dosho Port)
Dogen Zenji says, To listen to dharma is to cause your consciousness to play freely. This play is not a means but an end in itself. At that time you can have full commitment to play. The power to transform our life is from just playing with wholeheartedness. Naturally, you can play freely within practice. In the process of doing zazen, you can play freely with zazen and produce a new creative life. – Katagiri Roshi
I work in a school for teenagers in a large metropolitan district. Of course, like young people everywhere, they love to play. After a recent break, I asked a student how it was for him. He thought for a long moment and then said, "Well, I'd have to say good."
"What about it was good?" I asked. After another reflective pause he said in seriousness, "Well, I didn't get shot this time."
This young man is not alone. I see many young people whose lives have been shaped by violence, who have gunshot or knife wounds, some of them sport several such urban battle scars with pride. Almost all of them have close relatives in prison.
These young people reflect the larger culture as it appears through popular movies to computer games, a culture increasingly permeated by violence and its glorification. Many of us have become increasingly numb or oblivious to violence, tolerating more and more of the intolerable.
The popularity of violence might be due to how easy and cheap it is to get people's attention with violence—a lazy way that leaves us yearning for more in the hope that then we might feel alive, like potato chips and their greasy, saltiness that don't satisfy hunger but leave us wanting more.
What's this got to do with play?
In my view, the potential of Zen isn't limited to giving aging boomers something to do in their upper middle years, nor is it about meeting any individual's need for spiritual trips.
"The wind of Buddhadharma makes manifest the great earth's gold," said Dogen. In other words, Zen is about freshly addressing the key issues of our times and encouraging us to assertively make a Buddha out of a mud-ball life.
One of our primary issues, perhaps the challenge for our time, as Thich Nhat Han has long argued, is to make peace fun and interesting. Our survival may depend on it. Soto Zen, I suggest, is a practice of playing full out that offers one challenging way in which we can live a creative and deeply fulfilling life, doing what needs to be done with this precious opportunity of human birth.
Such a Zen has the potential to become a social movement, making playing together with all our hearts in all that we do the society's central organizing principle and our life's most passionate engagement.
Frequently Dogen emphasizes this point. For example, in "Fukanzazengi" he says, "If you concentrate your effort single-mindedly, that in itself is wholeheartedly engaging the Way."
And above you'll find Katagiri Roshi's comment on Dogen's word, yuge, transforming through play. "This play is not a means but an end in itself. At that time you can have full commitment to play. The power to transform our life is from just playing with wholeheartedness."
Yuge comprises the root of the name of our little practice place here in White Bear Township, Minnesota Yugeji, or Transforming Through Play Temple. Our most important guideline is to play full out.
By dropping the cowboy tendency to drift into our individual trips and our hungry ghost tendency to wallow in Zen-group-think blather, we are compelled to balance on a tight rope, on a razor's edge of dynamic aliveness. This is the life vein of vividly hopping along together in this great life.
It certainly beats getting shot.
(Editor's note: Dosho Port is a priest in Katagiri Roshi's lineage who teaches in Minnesota, author of Keep Me in Your Heart a While. Visit his blog: http://wildfoxzen.blogspot.com/)
(Jeffrey Burnaugh)
Dog Friends
(Sarah Webb)
My dog Rex, a big hound dog blue heeler mix, just one year old, loves it when I take him to the lake shore in the late afternoon. The lake is down so there are acres for him to run on, and there's water to splash through and birds to chase. He run almost out of sight, then thunders back and swerves as he gets to me, grinning with loopy joy. That's a way to be, I think, grinning back. Not the only way but a good way—charging forward, full heartedly, feeling your joy.
Rex running on the shore
Kids can be like that too—full hearted. Sometimes with joy, sometimes screaming their rage or sorrow. They run and stumble and leap right back up so they can keep on playing.
That's another way Rex is like a kid. He loves to play. About a month ago he met another dog along the shore, a furry-coated little beast about half Rex's size. He reminds me of Missy, my last dog, who died last summer, but this dog is bigger and much healthier.
After a few minutes of bristling, he realized Rex wanted to play, and they started to chase each other. We meet him almost every day now, and the two of them chase and tumble and nip and wrestle.
Kids will find the possibilities in a situation. A table? They'll sit at it, sit on it, crawl under it, tip it on its side, slide things down it, put a blanket over it, lie underneath it and look up.
That's what these dogs do too. Each day they find a new game. One will hide down in a hollow and burst out to surprise the other. They'll chew down sticks from the dried brush and tug-a-war with them. They'll steal the sticks from each other and race through the open with the other one in chase.
They leap into the ponds and splash about. They take a stick and trot together.
Yesterday we went further down the shore into heavy brush and they barreled through the brush in circles, apparently just for the joy of crashing about and following the leader. When they're exhausted—and it takes a lot to exhaust them—they'll lie on each other in a heap and pant. Then Rex will leap up and off they go again.
There's something about playing. Children play and dogs too, but so do many other creatures. Ravens do daredevil acrobats. Bear cubs wrestle and tumble. Lambs leap and run. Watching the dogs I see that it's more than delight in motion. There's a make-believe to it, as there is in children's play. I'll pretend to be angry and chase you and steal from you, but we know it's not for real. We act like we're separate and hostile, but we know we're not at all. Instead we are making something together, a game.
Are we one or are we two? the dogs ask, and their game says, one. One, and a one that is bursting out into creation, making not just this world but pretend worlds and painting worlds and hitting balls and catching them worlds, and for dogs, chasing and splashing and tumbling worlds. A one that has inherent in it, play.
Playing in Prison
(Lila Parrish)
Play shows up with surprising gifts sometimes. I volunteer at the state prison in Lockhart as part of Inside Meditation, the prison program that AZC is part of. The class I teach is on meditation and occasionally I share some circle dance as part of that. When the women come into the classroom and sit down they are carrying the energy of living in large "pods" or units and sharing a cell with another woman. There is no privacy and even smiling at someone can get them in trouble at times. As a result of that environment many of them have guarded faces. The circle dances are lively and the novelty of dancing brings down that guardedness. Their faces light up with laughter and the delight of moving safely and playfully together. When a dance ends I invite them to look around the circle and notice the difference in how their faces look. Next I ask them to share anything they would like. "I forgot I was in prison while I was dancing." "I felt like a child playing." Our dancing together can give some moments of freedom and of remembering a younger, happier self. Gifts of freedom and youthful energy for at least two women. And for me the gift of deep gratitude for their willingness to dance and play with me.
(Sarah Weintraub)
It is not sunny. Pieces of a to-do list keep bursting into my head and making me stand there thinking, instead of moving into the next yoga pose. I could've gotten up earlier today, really. And then gotten out of bed sooner, actually. We are out of milk, and everything, because my roommate didn't go grocery shopping. And she is the easiest person in the world to live with, by the way—how will I ever live with a partner? Here I am, again, on a thought, and forgetting which pose is next. And why don't more people know about the boycott against Coca-Cola for killing trade unionists in Colombia? And I really do need to sweep the house before the meeting here tonight.
And then there is the sitting still for a while, with a rakusu, cross-legged. Then I put a few dollars in my pocket and pull on my red suede Medellin boots and walk a block to the 24 Liquor Grocery, which is not actually open twenty-four hours a day, but is open on Monday at seven AM, which it is. On the walk, I am surprised by the pleasure of breathing, of breath entering and leaving, and by the pleasure of walking, of my muscles moving. It is not so cold out, and the birds go on and on and on, like it's springtime, which maybe it is.
We are not going to fail at our lives, you know.
I will lead a very interesting life. I will be very alive. I will enjoy it very much. I will keep doing my best—but my best to be kind, to all beings, starting with this one right here.
I will remember this; and I will forget it again. And I will remember and forget and remember and forget. Just like we return to our breath in zazen, I will return to this being at home in myself, to being relaxed and joyful, and to curiously relishing the unfolding of this life.
(Sarah Weintraub is the Executive Director of the Buddhist Peace Fellowship.)
Dash in Picture Frame
Introduction: Play (Rev. Trevor Maloney)
Remembering Play (Kim Mosley)
play at work, work at play (Kim Mosley)
Daylight Savings (Cristina Mauro)
The Ballad of Bodhidharma (John Grimes)
Showdown at the Genjo Corral (Peg Syverson)
Play Full Out (Dosho Port)
Playing (Jeffrey Burnaugh)
Dog Friends (Sarah Webb)
Playing in Prison (Lila Parrish)
Play (Sarah Weintraub)


Book pages : 16 Nos.
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Samsung Galaxy S20 Ultra Review
Samsung Galaxy S20 Plus Review
Samsung Galaxy S20 Review
Compact 2-TOUCH Display
64MP Telephoto Lens
A hybrid of a microSD Card Slot
Limited Storage
Samsung Galaxy S20, Design
The Samsung Galaxy S20 is the most affordable flagship smartphone under the Galaxy S20 range of devices. As for the other two models, the S20 is equipped with a first-class finish and build quality with a glass-to-metal sandwich design.
On the front side of the smartphone has a 6.2-inch TOUCH screen with QHD resolution, protected by 2.5 D curved Corning ® Gorilla ® Glass protection. There is a small punch-hole, or Such as-O-cut, with a higher screen-to-body ratio. In short, the phone looks premium and is light in weight. This is one of the first smartphones to support the 120Hz 2K AMOLED display.
Samsung Galaxy S20: The Performance Of The Hardware
The Samsung Galaxy S20, it is one of the first smartphones to be launched in India will be powered by the Exynos 990, SoC), based on the 7nm+ production, with 8GB of RAM and 128GB of onboard storage, with a hybrid SIM, card slot for additional expansion of the storage capacity of 1 tb.
The S 990 is a very capable flagship processor, it supports features such as 108MP camera, the native 8K video recording and much, much more. In addition to the ability to play a game that you throw at it with high-quality graphics, without any problem.
Samsung Galaxy S20: The Performance Of The Camera
The Samsung Galaxy S20 will come with a flagship camera setup, consisting of 64MP zoom lens, 12MP wide-angle lens, and a 12MP ultra-wide angle lens with support for 8K video recording at 24fps, and 4K video recording at 60fps.
When it comes to photography, the camera has a special night mode, and it features a 3x optical zoom, and may also be in the native 64MP images. For selfies, the smartphone makes use of a 10MP camera that can also record 2K videos.
Samsung Galaxy S20: The Price To Specs Ratio
The Samsung Galaxy S20 is available in India for Rs. 66,999, and it offers 8GB of LPDDR5 of RAM, a 128gb of internal storage. This device will compete against the likes of Apple's iPhone, as 11, which is also around the same price.
When it comes to price-to-specs ratio, its the Samsung Galaxy S20, it is still useful, because it provides a lot of features and does not compromise on the hardware specifications, it is compared with the high-end models.
Samsung Galaxy S20: Key Features/Highlights
The Samsung Galaxy S20's USP is that it comes with a 2K AMOLED display with a QHD resolution. This is not the first Samsung smartphone to offer a display with a 2K resolution, and it was one of the first smartphones to offer a 120Hz refresh rate which will be raise as the user of the smartphone experience.
The smartphone has a 4000 mAh battery with support for wireless charging, and a regular fast-charging. This phone also supports the reverse has an image stabilizer, which can be used for charging the battery, accessories and smartphones that support wireless charging.
Samsung Galaxy S20: Judgment
The Samsung Galaxy S20, the new age-the flagship smartphone from Samsung. This unit has everything a high-end smartphone user would expect to see. In addition, it also offers some of the industry-leading features, such as LPDDR5 of RAM, and a display unit that can provide up to 1200nits brightness, which will raise the user's smartphone experience.
If you are looking for a high-end smartphone that's compact and has all of the features, and it costs around a lakh is for the Samsung Galaxy S20 is the machine that you should consider.
Latest Samsung Galaxy S20 News
Get the latest Samsung Galaxy S20 news covering from compsmag. Daily updated Samsung Galaxy S20 phone news is written by the Compsmg team. Stay tuned for the latest information about Samsung Galaxy S20 phone, Samsung Galaxy S20 operating systems, Samsung Galaxy S20 Specs. Check out Latest News Of Samsung Galaxy S20
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UI instructors move courses online, despite campus rules
Home / News / Community
Stop the Violence event set for Saturday in Cedar Rapids
Kat Russell
CEDAR RAPIDS - It's been roughly two weeks since Cedar Rapids saw a weekend with five shots-fired reports, and Brandon Jackson, an organizer of this year's annual Stop the Violence event, said it's time to put the guns down.
'This isn't just a neighborhood problem," he said. 'It's a city problem. It's a problem that affects everyone."
Jackson, 33, is the founder of Dreem Sports, a local nonprofit youth sports organization that started in February. This is Jackson's first year organizing the Stop the Violence event, and this year he said will be a little different.
The event is scheduled to begin at noon Saturday at Redmond Park, 1545 Third Ave. SE. Jackson said the event will start with sign-making followed by a march through the city at 1 p.m. Event organizers are asking that attendees wear red.
'After the march, we'll all gather back at the park, and there will be food and activities and music and games for everyone to enjoy," Jackson said.
The goal of the event, Jackson said, is to raise awareness about the violence plaguing the community, to promote community togetherness and to remember those lost to violence.
'We want everyone to come out," he said. 'We'd like to see community members and leaders, city officials, churches and other organizations come out and get involved in working to end the violence."
Jackson said he would especially like to see organizations and agencies that are offering youth and teen summer programs come to event and let people know what they have to offer kinds in the neighborhood.
The event come roughly a week after a particularly disturbing weekend that brought five shooting incidents in the span of 55 hours.
Cedar Rapids Public Safety spokesperson Greg Buelow said four of the weekend incidents in the Wellington Heights neighborhood appeared to be related.
'In many cases, there are individuals that are engaging one another in ongoing disputes," he told The Gazette last week. 'These groups are hybrid gangs. They don't contain the traditional structure of historic criminal street gangs. These are generally loosely affiliated and often change their allegiance to one another. They are often in disputes about relationship issues or other perceived grievances. Unfortunately, some have access to firearms and are using them to attempt to settle their disputes."
A lifelong Cedar Rapids resident, Jackson said he grew up in the Wellington Heights neighborhood.
'When I was a kid, it felt safe," he said. 'We could run around and play outside or walk to the corner store to get ice cream and our families didn't have to worry about us."
Now, a father himself, Jackson said he wants better for his children.
'It's the youth who are most affected by the violence," he said. 'They're the ones getting shot and doing the shooting, and they're getting younger and younger. We have to do better for them. We have to come together as a community and deal with this issue head on."
'This event is about coming together as a community," Jackson said. 'We want to celebrate life, unity and community and we want to remember those we have lost."
l Comments: (319) 398-8238; kat.russell@thegazette.com
Jimaine Cooper of Cedar Rapids delivers hot dogs at the 2014 Stop the Violence event in Redmond Park in Cedar Rapids. This year's march is Saturday, and organizer Brandon Jackson is calling on people and organizations to come together to end the spate of shots-fired incidents plaguing the city. (The Gazette)
Antionia Bradey (right) of Cedar Rapids dances at the 2014 Stop the Violence event at Redmond Park in Cedar Rapids. This year's march is Saturday. (The Gazette)
Preview, Daily Report, Criminal Cases
Reporter, The Gazette
Public safety reporter for The Gazette.
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We bring a nice team of horses to Dundalk tonight and we run three in the seven-furlong 2-year-old Median Auction Maiden at 6 o'clock.
Niall rides Kasriyann who was unsuited by the soft ground, but he still ran a respectable race on his debut at Limerick. He has improved since and we just gave him a little chance after that.
He has been to Dundalk and he handles the surface well and despite a middle draw in 8, I think he'll run a very big race.
Total Recharge (Ross Coakley) is a horse we like a lot. He is well drawn and will improve plenty for the experience. He has been to Dundalk and handled the surface well, he is ready to start.
Libras Power gets the fillies allowance and we are taking an extra 5lbs off her back with Ben Coen and she has come forward from her first start when she was a promising fifth. She likes the surface and should run a good race.
She could have been drawn a bit better, but we can't do much about that and we've been happy with her work and she is capable of running a big race.
La Novia (Ross Coakley) gained a deserved success the last day at Dundalk and she bids to follow up in the apprentice handicap.
She likes the surface and has a reasonable draw and while she got 7lbs for her victory here the last day, that would have done her confidence the power of good and she goes there with every chance.
Finally, Jasikan which won his maiden at Dundalk recently was sold to continue his racing in America. He's a lovely colt and we wish his new connections well.


Corbitt & Sons Construction Co. Inc., 8728 Robbins Road, 46268, chapter 11 reorganization, liabilities: n/a; assets: n/a.
Allison Kay LLC, 7834 N. Michigan Road, 46268, chapter 11 reorganization, liabilities: n/a; assets: n/a.
Townsend Research Laboratories Inc., 1399 Sadlier Circle West Drive, 46239, chapter 7 liquidation, liabilities: $677,775; assets: $64,734.
March 12-14 Indiana Safety and Health Conference & Expo, Indianapolis Marriott East. Contact: Bob Dittmer at 234-3793. The event is presented by the Central Indiana Chapter of the American Society of Safety Engineers and the Indiana Chamber of Commerce and is in partnership with INSafe of the Indiana Department of Labor and the Indiana Association of Occupational Health Nurses. Contact www.INsafetyconf.com.
Indiana Members Credit Union and The Children's Museum, provided a night of fun, and interactive learning about fitness and health for students and families at IPS School 44.
Meals on Wheels to benefit through the month of March from the sale of Foodie Indy dining cards which offer $10 off a purchase of $30 at 52 participating restaurants. Meals on Wheels receives $10 of the $20 cost of the cards. Contact: 252-5558 or info@mealsonwheelsindy.org.
Janus Developmental Services to benefit from the 4th annual Create, Connect and Commit Ask Event March 23 at 7:30 a.m. at the Ritz Charles. Tickets: $1,000 for a 10-person table. Contact Joanne McDonough at 773-8781, ext. 112 or jmcdonough@janus-inc.org.
Indianapolis Senior Center to benefit from its 25th annual Senior Prom on April 13 at 7 p.m. at the Indiana Roof Ballroom. Tickets: $45-$60. Contact 263-6270 or yourcenter.org.
Assistance League of Indianapolis to benefit from its annual Star Gala on April 14 beginning at 5:30 p.m. at the Ritz Charles. Tickets: $110 for individuals, $175 for patrons. Contact 872-1010 or StarGala@alindy.org.
Indy Reads to benefit from its annual Alphabet Affair on April 21 at 6 p.m. at the Indianapolis Hyatt Regency Hotel. Tickets: start at $100 per person, $1,700 for corporate tables. Visit: indyreads.org/alphabetaffair.html for tickets and information.
Meredith Setser will receive $20,000 as the winner of an Efroymson Contemporary Arts Fellowship.
Horizon House received grants of $25,000 from the Nina Mason Pulliam Charitable Trust, $25,000 from the Richard M. Fairbanks Foundation and $5,000 from the Herbert Simon Family Fund, a fund of the Central Indiana Community Foundation.
Kiwanis Foundation, Safe Sitter, Prevent Child Abuse Johnson County, Project Concern and the Domestic Violence Network each received grants of $25,000 from Lushin & Associates, as well as a Key Management Dynamics Assessment.
Kyle Niederpruem was named The Bob Jackson Volunteer Award winner by the Indiana Debate Commission for her extraordinary service.
Forty-eight attorneys at Ice Miller were named 2012 Indiana Super Lawyers and 18 were named Rising Stars by Super Lawyers magazine.
Kathy Lucas, Bose McKinney & Evans LLP, received the 2012 Meritorious Service Award from Indiana Pork.
Citizens Energy Group is the 2012 United Way Jefferson Corporate Employee Volunteer Program of the Year recipient, Fairbanks is the Nonprofit Volunteer Program of the Year recipient and Rose Bailey, Rick Turner and Bob Skinner are recipients of Live United Volunteer Programs of the Year awards.
Jim Barnes, president and CEO of enVista, was selected as a 2012 Pro To Know by Supply & Demand Chain Executive magazine.
Eight attorneys at Harrison & Moberly LLP were named 2012 Indiana Super Lawyers and two attorneys were named Rising Stars by Indianapolis Monthly and Law & Politics magazines.
David Harris of The Mind Trust was selected by Teachers' Treasures as the recipient of its Caring Spirit Award.
Signarama in Carmel was certified as a green business by the Greater Indianapolis Chamber of Commerce.
RL Turner Corp. was named General Contractor of the Year by the Indiana Subcontractors Association.
Drew White of Axis Architecture was elected into the American Institute of Architects College of Fellows.


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Extra! Extra! Read all about it! This day at MEVO we will be talking about the B Hive Salon's –Bergen County's first organic full service boutique salon–Second Annual Earth Week Event. This event will be a Green Fair hosting, among other things: henna tattoos, homemade honey, organic hair chalk and braid bars, Bergen County's Habitat for Humanity, a new Chevy Spark (that's right a car), music from Waldwick's School of Rock, energy saving tips, Bergen S.W.A.N, the Purple Dragon Co-op, and of course, Mahwah's own MEVO! The event will be held on April 28, 2013 from 10:00am-3:00 pm at 269 Broadway at Hillsdale, NJ, the location of the B-Hive salon!
And for those interested in B-Hive's organic products check out their website www.bhivesalon.com or call 201-470-HIVE to make an appointment. In the meantime though, enjoy the fair, cause MEVO will see you there!


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8 Years Ago Today, Tyler Seguin Became A Dallas Star
July 4, 2021 ~ Shortman Hams
Not much happening in sports today because it's the Fourth of July but we do have something else to celebrate. 8 years ago today, Tyler Seguin was traded to the Dallas Stars from the Boston Bruins. In his 8 years in Texas, Tyler has played in 541 games and brought out 516 points (225G 291A) while averaging 19:40 on the ice per game. He has played in 46 playoff games with 27 points (7G 20A) while averaging 19:21 on the ice per game.
Seggy has got a career Corsi For of 59.8%. He averages 4 on ice goals for per 60 minutes, then he averages 2.5 goals against per 60 minutes. Tyler has been one of the most influential players to hit Dallas's roster. He leads the team on and off the ice, he's a solid player for them, it's a trade that is always going to be massive to Dallas's success.
Without Seguin, the Stars never make that Stanley Cup Final last year and if he wasn't injured for 95% of this year, they might have been right back in that race. It's definitely a day Stars fans should celebrate. Now, before I throw on Miracle, here's some Seggy content to keep you happy.
Lest We Never Forget:
(As a queer person, I don't think its homophobic. I think this is the best god damn sentence ever written, I want it on a t-shirt.)
Featured Image: Ronald Martinez/Getty Images
Posted in Dallas Stars, Hockey, Men's Hockey, NHL	Dallas StarsHockeyMen's HockeyNHLTyler Seguin
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