I first encountered him in the Autumn 3 anni fa.
I am truly glad to have met her. It was difficult playing Misato Katsuragi even during the TV series — because she is a person who doesn't easily speak her true feelings. During scenes where her feelings exploded or she poured out her heart, I also became a bit over-emotional and afterwards couldn't remember exactly what kind of performance I had given (- not a good thing). My hands shook and it took all my might to keep the script I was holding from rustling and making noise. (Times like these make me feel that voice acting is a bit restricting.) I have focused exclusively on Misato for so long — wanting to know her, to get close to her — concentrating all of my five senses on her. That's the way I am, so I am unable to objectively look at "Eva" right now after finishing the voice-over work. My perspective is still on the same level as Misato, but I feel that's fine. In episode 25′ "Aria" she was strong, brave and a woman. The sole survivor of Second Impact 15 years earlier, her cross necklace is the keepsake of her father. I wonder if it's just me who feels that she alone survived in order to give that cross to Shinji?
But it is not futile or thankless work. Trying to understand and become closer to others is a wonderful thing. I think that the "desire" to understand is different from just "feeling" that you want to understand. For this reason there is no need to do it "bene". The more unstable you are, the more you worry about other people, and how they view you. But that's okay, because you are you. Whatever you see, hear, are recommended, or eat, you are free to decide what you take into and expel from your body. Whether a person lives in a grandiose manner or simply seeks pleasure depends on that person. Intense happiness and quiet happiness also depends on that person. Deep in my heart I have a still, unwavering, indescribable and invisible "place" where I face myself and reaffirm that "I want to live." But that doesn't mean that I want to promote this way of life or say it's wonderful at all. I am only facing myself. I am different from other people, but that is why I sometimes want to be or do like others. (like the Instrumentality Project?) Sometimes I even try to force my will onto others, saying that something should be a certain way. I feel that wanting to do so is fine, but actually doing it is not. Dopotutto, that is coercion — compulsion. Further, it feels good to want to be like each other. It is very comfortable when you are with another person whom you have come to know well and have this feeling with. I want to meet many people like this. Finally I want to say that I gained many things from Eva.
Grazie, good night, and remember – the things you can see aren't everything.
Evangelion has finally reached its conclusion…. Congratulations, everyone, on a job well done. A, veramente — Thank you very much. 24 years ago as I gave my birthing cries in Kobe, surely not even Admiral Isoroku Yamamoto of the combined fleet or Nostradamus could have predicted that I would participate in the project called Evangelion. Evangelion's popularity is unstoppable — like the raid on Pearl Harbor. I'm sure that every Eva fan with a Japanese Spirit will feel like singing none other than "Off to Sea" a partire dal "Sally Forth" as they watch this conclusion. If so, then I'd like to send everyone to the theater with a big cheer of "Banzai!" In case you couldn't tell, I think I had a "Kamikaze" feeling during the voice-over. (heart) Haha… (heart) Well done everyone.
A younger voice actress whose first regular appearance was in Evangelion. Before that her main activities were puppet plays and other stage productions. Her other roles include Mikemi in "Food by the Bucket" and Kachusha in "Wedding Peach", eccetera.
Ritsuko fades away with her final word, "Liar." But what was this "Bugiardo" in reference to? The script for this last scene only has Gendo saying: "Ritsuko Akagi, Io veramente…." followed by Ritsuko saying: "Bugiardo (gets shot)". I can imagine many words that might follow "Io veramente….", but I can't decide on any in particular. That is the complexity of Gendou and Ritsuko's relationship.
Feeling unsatisfied with this, I looked for a way to accept her death at the hands of Ikari. This made the interpretation of "Bugiardo" very important. But the voice-over grew nearer and nearer….
Director Anno must have noticed how I felt. When it came time to do the voice-over, he showed me a single, hidden hint at the last moment. With that one incredible hint, io, and Ritsuko Akagi, were utterly defeated. It hardly needs saying, but Director Anno is incredible. Truly awesome — a genius. I am truly grateful for the two and a half years that I have been involved with this project, and I would like to take this chance to thank from the bottom of my heart everyone in the staff and cast who helped and supported me when I needed it.
Active as a narrator for TV commercials, Fumihiko Tatsuki specializes in 'funky' ruoli. Representative appearances include the reporter Yanagishita in "Miracle Giants – Doumu-kun" and Uncle Bob in "HARELUYA II BØY". He is also active as a singer.
From the TV series to the spring theater release (D&R), and now to this conclusion — normally this would be like plodding along toward the peak of a steep mountain. Inside of me, però, il "days of maturation" over this period declined against my will, leading me to confirm anew the fickleness of my soul.
Motomu Kiyokawa has worked on numerous anime and foreign TV dramas, and is also active as a stage actor. He has also played numerous monster roles in SFX productions. Other appearances in GAINAX works include Gargoyle in "Nadia – Il segreto di acqua blu", and he is also the voice of Professor Mikamura in "Mobile Suit G Gundam".
I was riding the subway about the time of the spring theater release (D&R), and overheard three junior high school aged kids discussing Evangelion. The discussion basically took the course of: "That part means such and such," "A, I disagree," and so on. There haven't been very many anime works that people have really discussed, and I think this is one of the great things about Eva.
I feel that even when acting on stage, the type of drama that makes the audience feel and think various things is interesting drama, and also good drama. Ovviamente, the audience won't imagine anything if the drama is devoid of content. Director Anno created many such places in Evangelion where the audience can imagine things. That's why I think it is great.
The voice-over for the cinema edition is finished, and so is my work as an Evangelion voice actor. All that is left now is to view the finished product. I look back on the first episode of the TV series with fond recollection. We actors seem fated to approach each work with an attitude of extreme indifference. As soon as one job finishes, we must turn our thoughts to a different job. Tuttavia, I was been able to be involved with Evangelion for a long period from the TV series to the first and now the second movie, although there was some interval between each. This has led me to develop a deeper attachment to my character. Makoto's confession of affection toward his superior… that scene left a deep impression. Inside myself I ruminated over the different things that he must have thought, must have felt…. I think that because I could be involved for so long, I was able to let my heart roam freely. Personally, I gained many things from the experience, although I'm not sure whether these appeared in my acting.
Tuttavia, isn't all progress and invention ultimately made by people who deviate from society and are thus not "normal"?
Tuttavia, the boundary between "normal" e "abnormal" in the anime industry is currently changing.
Fertile imaginations and somewhat "abnormal" pensiero.
Representative works include Maria in "(Yuusha Shirei) Dakuon" and Palla Sith in "Mobile Suit Gundam X". Many of her roles are as active young girls. Recently she has been active as a personality on the radio program "Ayakashi radio kunoichiban", eccetera.
Well known as the voice of younger characters in foreign TV dramas such as Wesley in "Star Trek The Next Generation" and Lucas in "Sea Quest", he has also appeared as Fish Eye in "Bishoujou Senshi Sailor Moon SS", eccetera.
Luckily, però, this time I was able to associate myself with Evangelion as Kaworu to the very end. Was Kaworu's choice correct? Did Kaworu really "keep on living"? While this answer appears to have been entrusted to Shinji, I am honestly happy to have been lucky enough to see it through to the end with my own eyes.


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text:
Home/News & Gossip/Cardi B Pleads Guilty To Strip Club Assault Charges – Says She's 'Growing Up'
Cardi B Pleads Guilty To Strip Club Assault Charges – Says She's 'Growing Up'
Cardi B has pleaded guilty in her long-standing assault case — and she says the whole experience has helped her mature.
As you may remember, the rapper got in a fight with Offset's rumored side chick at Angels Gentleman's Club in New York City way back in 2018. It was reported she ordered a beatdown on TWO employees, who were bartenders and NOT dancers! Before the attack in question, Jade — one of the bartenders — says Cardi was threatening her for months because the musician believed she slept with her husband. After getting a warning the I Like It rapper was targeting her, the bartender says five people associated with the star grabbed her hair, punched her face, and attacked her with an ashtray.
Related: Erika Jayne CLAPS BACK At Jennifer Lawrence For Calling Her 'Evil'!
In 2019, the Up rapper was hit with a misdemeanor summons. She chose to go to court rather than accept a plea deal back then, but it would seem she's changed her mind as of Thursday. According to WNBC, the 29-year-old pleaded guilty to third-degree assault and second-degree reckless endangerment. She admitted to offering a friend $5,000 to beat up the victim on her behalf and throwing bottles in the direction of the bar worker and her sister. Because of this, she will have to do 15 days of community service, but will avoid jail time.
The Bodak Yellow musician doesn't seem to have an issue with her time in court, though. In a statement to E! News following her guilty plea, she admits she's learned a lot from the situation and that she's "growing up":
"Part of growing up and maturing is being accountable for your actions."
Continuing, the rapper says she wants to set a good example for her children:
"As a mother, it's a practice that I am trying to instill in my children, but the example starts with me. I've made some bad decisions in my past that I am not afraid to face and own up to."
It's wonderful she's always trying to be the best person she can be for her kids! The 29-year-old says her past actions "don't define her" as she moves into the future:
"These moments don't define me and they are not reflective of who I am now. I'm looking forward to moving past this situation with my family and friends and getting back to the things I love the most—the music and my fans."
We're glad Cardi seems to be in a much better place now and is taking responsibility for her actions. What do U think about her guilty plea, Perezcious readers? Sound OFF in the comments (below).
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'Law and Order: SVU' Recap: Did Benson and Stabler Kiss? Season 24

redpajama_set_name: RedPajamaCommonCrawl


text:
- Szeretnénk tankolni. Hol a legolcsóbb a benzin környéken?

## Screenshot
![screenshot](http://i.imgur.com/tsaKuLE.jpg)

## Details
- 5 km-en belül a 3 legolcsóbb felsorolva, a harmadikkal kezdve, majd második, végül az első helyezett (legolcsóbb).

	- Kúton a benzin ára
	- Kút "márka" neve (pl: OMV, Magán, Shell, ..)
	- Kút távolsága és annak pontossága.
	- Kút pontos címe. (Város, kerület, utca, házszám).

	- Lehet egy gombbal frissíteni az adatokat.
	- A "frissítés" gomb ad visszajelzést, ha megnyomódott.
	- Az adatok frissítésének idejét is ki írja, másodperc pontosan.

![screenshot](http://i.imgur.com/6P3mf4o.png)

## Licence
- Rapcsák Dénes
- Istenes Imre
[MIT license](https://tldrlegal.com/license/mit-license).

redpajama_set_name: RedPajamaGithub


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text:
Fidel Castro quotes
Fidel Alejandro Castro Ruz was a Cuban politician and communist revolutionary who governed the Republic of Cuba as Prime Minister from 1959 to 1976 and then as President from 1976 to 2008. Politically a Marxist–Leninist and Cuban nationalist, Castro also served as the First Secretary of the Communist Party of Cuba from 1961 until 2011. Under his administration, Cuba became a one-party communist state, while industry and business were nationalized and state socialist reforms were implemented throughout the society.
Nickname: Fidel Alejandro Castro Ruz
Profession: Lawyer
governed
Even Gaddafi's adversaries assure us that he stood out for his intelligence as a student; he was expelled from high-school for his anti-monarchic activities. He managed to enroll in another high-school and later graduated in law at the University of Benghazi at the age of 21.
I think that a man should not live beyond the age when he begins to deteriorate, when the flame that lighted the brightest moment of his life has weakened.
Atal Bihari Vajpayee is an Indian politician who was the 10th Prime Minister of India, first for 13 days in 1996 and then from 1998 to 2004. A leader of the Bharatiya Janata Party , he is the first Prime Minister from outside the Indian National Congress party to serve a full five-year term. At age 93, Vajpayee is currently the oldest living former Indian Prime Minister.
Anthony Carmona
Anthony Thomas Aquinas Carmona, SC, ORTT is the fifth President of Trinidad and Tobago, in office since 2013. Previously he was High Court Judge at the Supreme Court of Trinidad and Tobago, and he served as a Judge of the International Criminal Court from 2012 to 2013.
Starlet Marie Jones is an American lawyer, journalist, television personality, fashion designer, author, and women's and diversity advocate. She is best known as one of the original co-hosts on the ABC morning talk show The View, on which she appeared from 1997 to 2006. She was also one of sixteen contestants of the fourth installment of The Celebrity Apprentice in 2011, coming in fifth place.
Randy Neugebauer
Robert Randolph "Randy" Neugebauer is an American politician who was the U.S. Representative for Texas's 19th congressional district, having served from a special election in 2003 to 2017. He is a member of the Republican Party. The district includes a large swath of West Texas, including Lubbock and Abilene. According to a 2011 survey by the National Journal, Neugebauer is "the most conservative" member of the House.
Golda Meir was an Israeli teacher, kibbutznik, stateswoman, politician and the fourth Prime Minister of Israel.


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With one baby in the house and another on the way, Jason Aldean's life is rapidly changing these days—in ways he never could have anticipated back in the younger days of his career.
Aldean told Taste of Country Nights back in May that he appreciates being able to take his little boy out on the road with him, something that wasn't possible when his two older children were younger. "Back when [my daughters] were born, when I was touring I was on one bus with my whole band—there was 12 of us on a bus," he explained. "So I wasn't in a position to be able to carry them out a whole lot back then."
Aldean and wife Brittany are expecting their second child together, a daughter—so Memphis (who turns 1 in December) will soon have a buddy to play with out on the road.
Aldean's remarks were prior to his performance at the 2018 iHeartMusic Festival in Las Vegas Friday (Sept. 21). This marks almost a year since he played the city Oct. 1, 2017—the night a shooter opened fire on the crowd during his set, killing 58 people.


text:
About West Parish Garden Cemetery
The Chapel at West Parish
Children's Garden Area
Cemetery Grounds
Children's Garden
Cremation / Columbarium
Memorialization
Perpetual Care
Samuel Blanchard, 1707, April 22— The oldest epitaph and burial in the cemetery. Here lyes ye body of Samuel Blanchard who departed this Life on ye 22, of April 1707 in ye 78th year of his age. Ye memory of ye just is blessed. Samuel Blanchard's home was on the site of 36 Haggetts Pond Road, 2 ½ miles from the cemetery. Samuel was a Proprietor and house holder before 1681, Selectman in 1687.
Deacon Soloman Holt, 1888, April 3, age 84— Deacon and founding member of West Parish Church, 1826, July 11, Solomon Holt sells to the Proprietors of the Meeting House westerly side of the Shawsheen River 1 acre of land to build the church. Solomon Holt's home and farm was 111 Reservation Road. A plan of the farm Holt by Nathan Abbott 3rd, July 14, 1847 shows 131 acres. Currently all the land contained within the cemetery with the exception of the original 1 acre burying ground and all the land that now comprises the West Parish Meadow AVIS Reservation east of Reservation Road was once part of Holt's farm.
John Smith, 1886, Feb. 25 age 89— Co-Founder of Smith & Dove Manufacturing Company 1836 first flax mill in United States, philanthropist, abolitionist, established Free Church in Andover, founder of Andover Memorial Hall Public Library.
Peter Smith, 1880, July 6, age 77— Co-Founder of Smith & Dove Manufacturing Company 1836 first flax mill in United States, philanthropist, Deacon of West Parish Church.
Peter Dove Smith, 1911, Jan. 16 age 69— son of Peter Smith, President of Smith & Dove Manufacturing Co. Selectman, Deacon of West Parish Church and cemetery board treasurer. Civil War veteran, wounded at the battle of Spottsylvania in 1864.
Benjamin Franklin Smith, 1908, Feb. 27 age 29— grandson to Peter Smith, Treasurer of Wood Mill & Assistant Treasurer to American Woolen Company. Memorial Arch at entrance of the cemetery is dedicated to his memory.
Susanna Smith Purdon, 1882–1974, age 92— granddaughter of Peter Smith, West Parish Church member, philanthropist. Bequests established Smith Purdon Fund of West Parish Church and the research library and achieves of the Andover Historical Society.
Mary Byers Smith, 1983, Feb 1, age 97— daughter of Joseph W. Smith and grand daughter of John Smith, 1st woman to serve on the Andover school committee, Graduate of Smith College 1908. trustee of Memorial Hall Library, founded the Hampshire Bookshop in North Hampton 1916, trustee of New England Hospital for Women and Children, treasurer of Orchard Home for Girls in Boston. Volunteer with the state Department of Social Services as well as well as helping to set up libraries in hospitals and prisons.
Esther W. Smith, 1952, Oct. 15, age 81— daughter of Peter Dove Smith, Policewoman in Andover for 40 years, and excellent marksman with a rifle, active in town affairs, member of West Parish Church and the Andover Garden Club.
William Madison Wood, 1926, Feb. 2, age 68— President of American Woolen Company, the largest woolen company in the world, philanthropist. Enlarged and redesigned West Parish Cemetery 1908, created Shawsheen Village 1918–1924 model community, Andover Country Club and Arden Trust.
Ellen Wheeton (Ayer) Wood, 1951, July 6 age 91— wife of William M. Wood, daughter of Frederick and Cornelia (Wheeton) Ayer of Lowell, MA. philanthropist.
William Madison Wood Jr., 1922, August 15 age 30,son of William Wood, Vice President of American Woolen Co. WWI Navy Veteran. Founder and President of Edington & Co.
Cornelius A. Wood, 1972, May 13, age 78— son of William M. Wood, 2nd and 1st Vice President & President of American Woolen Company. President and director of the Park Square Building Co., Boston, president of Shawsheen Realty Co. Veteran of WWI & WWII.
William Madison Wood III, 2005, March 19— Grandson of William M. Wood, veteran of W.W. II
Rev. Cornelius Ayer Wood Jr., 2005, June 5, age 84— National Institute of Public Affairs, (fellow) Washington, executive secretary to Federal Security Administrator 1947-1951, work with the Episcopal Bishop of Washington, and affiliations with American Arbitration Assoc. of New York, Penn Community Services Inc. St Helena Island, SC. New York Society of Security Analysts, Boston Society of Security Analysts, Washington Society of Investments Analysts & the Financial Analysts Federation.
John Franklin, 1942, May age 74— Civil engineer, Chief engineer of the American Woolen Company, Designer of the layout of West Parish Garden Cemetery 1908, the stone bridge and all the landscaping. Designer of Shawsheen Village 1918–1924.
George C. Shattuck, 1923, Sept. 23— Andover native, Architect, designed the Arch, Chapel, Tomb and Stone cottage of West Parish Cemetery while draftsman at Shepley, Rutan & Coolidge of Boston. In 1915 he became a partner in Coolidge and Shattuck after the deaths of Shepley and Rutan. Received medal of the Boston Society of Architects for the Boston Lying-In Hospital.
Frank H. Hardy, 1946, Jan 30, age 75— Co-founder of the Hardy Brush Company, town Selectman, member of West Parish Church, instrumental in development of the cemetery and building of the American Woolen Company mills in and construction of Shawsheen Village.
Charles A. Hardy, 1913, Dec. 23, age 72— Father of Frank H. Hardy, Co-founder of Hardy Brush Company. Veteran of the Civil War 13th District of Columbia Volunteers.
Perley F. Gilbert, 1956, May 5, age 87— Architect and head of firm Perley F. Gilbert Associates of Lowell, MA. , graduate of Punchard 1887, Phillips Academy, MIT, designer of Andover Junior High and Memorial Auditorium (now Andover School Administration buildings), Orlando Cottage – The Lanam Club, and West Parish vestry 1926.
Granville K. Cutler, 1933 May 24, age 67— Purchasing Agent for American Woolen Company for 35 years, member of School Committee and town finance board, overseer of cemetery expansion and Chairman of the cemetery board 1908–1933.
William A. Trow, 1947, Nov. 20 age 80— Established Armstrong Knitting Mills in Boston, School Committee and Deacon of West Parish Church. Cemetery board member and past President of Andover Historical Society and Punchard School board of Trustees.
Frederick S. Boutwell, 1939, Feb. 16. age 74— Treasurer and Vice President of the Andover Savings Bank, Director of Memorial Hall Library, member of West Parish Church and instrumental in the 1908 expansion of the cemetery. Cemetery board member and treasurer 1910–1930.
Arthur T. Boutwell, 1940, Sept. 1, age 72— Chemist with Smith & Dove Company and the Ludlow Association, Chairman of the cemetery board 1933– 1940.
Edward Francis Abbott, 1913, April age 61 —Member of West parish, Deacon from 1894–1913, also served as sexton, collector for the parish and Superintendent of the cemetery.
Albert B. Burtt, 1923, May 3, age 53— 1st Superintendent of West Parish Cemetery 1916–1923.
Robert Scobie, 1952, May, 6, age 75— 3rd Superintendent of West Parish Cemetery 1928–1952. Born in Dundee, Scotland came to Andover in 1923, Member of West Parish Church, and St. Mathews Lodge A.F. & A.M.
Arthur R. Lewis, 1981, Nov. 10, age 86— 4th Superintendent of West Parish Cemetery 1953–1978, Andover School committee 12 years, Veteran of W.W.I. and Deacon and Sexton of West Parish Church.
Frances L. Dalton— Artist, teacher and Andover native, listed in Who's Who of American Women Artists.
Charles Upman Bell, 1922, Nov. 11, age 79— Justice of Massachusetts Superior Court 1898–1922.
Rev. James G. Merrill D.D. Minister— Editor of Christian Mirror, President of Fisk University. Son of Rev. James H. Merrill.
Henry Flint Chandler, 1835–1906 – age 71— Medal of Honor – Civil War Sergeant Co E 59 Mass. Infantry.
Willie Carleton Richardson, died October 23, 1909 age 10y. 11m.— son of A.P. Richardson 73 Park Street. Interment was in West Parish Cemetery, he being the first one carried under the new arch since its completion. Willie was a pupil at Stowe school, member of the Intermediate department at South Church and was a member of the Christ Church choir under the leadership of John Bachelder. He died of Peritonitis a result from a fall seven weeks earlier.
Fred H. Henry, 1910 May 25— of Wakefield, formerly of Andover, age 56 died in Philadelphia. Funeral service was held Wednesday afternoon in the chapel in the West Andover cemetery. Mr. Henry was the inventor of a type of car seat generally used in street railway cars. This is the first written account of the chapel being used.
Telemachus "Mike" Demoulas, 2003, May, age 82— Co-founder of the Demoulas Market Basket grocery chain stores and philanthropist. Established the Demoulas Foundation.
Jacob Warren Barnard, 1833–1903— Shoe manufacturing, investment and Real Estate agent, largest land holder in Andover in 1903, Chief Director of the Andover Electric Company, Andover Home for the Aged. Established the Barnard Prize for Composition at the high school.
Arthur W. Cole, 1895–1981— Director of Andover Public Assistance 1936–1961, Trustee of South Church Ministerial Fund and Punchard Free School, Cornell Fund and past President of Andover Home for the Aged. Veteran of WWI.
Walter E. Pike, 1958, Dec. age 82— Treasurer of W.N. Pike and Sons of Lawrence, the company hired in the construction of the cemetery.
Cynthia E.Pike— Teacher and educator, Founder of the Pike School in 1926. Wife of Walter E. Pike.
Rev. James H. Merrill 3rd— minister of West Parish Church April 30, 1856 – Dec. 1, 1879, 23 years and first parish minister buried at the cemetery. Born in Lyndeboro, NH October 16, 1814, Graduate of Dartmouth College 1834, Principal of Academy of Fryeberg, Maine 1834- 36, Graduate of Andover Theological Seminary 1839, Pastor Montague, MA 1839–1856, Married to Lucia Wadsworth Griswold of Fryeberg. Children, Rev. James G., William F, Sara E., Lucia G, & George C. Died October 28, 1886 age 72.
Rev. Donald H. Savage— 11th Minister of West Parish Church Sept. 1, 1937 – March 6, 1945. Born in Pennsylvania September 1, 1907. Graduate of Wesleyan 1929, Boston University School of Theology master's degree in1931 and S.T.B. degree in 1932. Pastor of Trinitarian Congregational Church in Norton, MA prior to West Parish. He died in the service of the Parish on March 6, 1945 at the Middleton sanatorium of Tuberculosis. Age 37. Married to Adele Brodhead, two children Robert and Jane.
Rev. Newman Mathews— 10th Minister of West Parish Church July 1913 – June 1937 and Minister emeritus 1937–1951. Born in Drayton, Ontario. Graduate of Phillips Academy 1894, Harvard College 1897, University of Toronto and Bangor Theological Seminary. Ordained in Scranton, PA, Pastor in Randolph, NY, Kane, PA and St Petersburg, FL before West Parish. Married to Elizabeth Tomlinson. one son Medwin Mathews. Died November 7, 1951 age 82.
Alexander Ezekiel Cohen— died April 14, 1888. born July 4, 1817, Lipol Poland, son of David & Minda age 74y 11m. and his wife Ann Cohenwho died July 10, 1872. The Cohens are the first Jewish family to be buried at West Parish.
Veterans of the Armed Services— Veterans of all major wars and branches of service are interred at West Parish Garden Cemetery. They are notable for their service to their country and their sacrifice to defend our freedom and liberty. Their graves are marked with American flags on Memorial Day and Veterans Day in tribute to their service and as a visual reminder to all who come to pay respect to loved ones lost.


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Project acronym 3D-JOINT
Project 3D Bioprinting of JOINT Replacements
Researcher (PI) Johannes Jos Malda
Host Institution (HI) UNIVERSITAIR MEDISCH CENTRUM UTRECHT
Call Details Consolidator Grant (CoG), LS7, ERC-2014-CoG
Summary The world has a significant medical challenge in repairing injured or diseased joints. Joint degeneration and its related pain is a major socio-economic burden that will increase over the next decade and is currently addressed by implanting a metal prosthesis. For the long term, the ideal solution to joint injury is to successfully regenerate rather than replace the damaged cartilage with synthetic implants. Recent advances in key technologies are now bringing this "holy grail" within reach; regenerative approaches, based on cell therapy, are already clinically available albeit only for smaller focal cartilage defects. One of these key technologies is three-dimensional (3D) bio-printing, which provides a greatly controlled placement and organization of living constructs through the layer-by-layer deposition of materials and cells. These tissue constructs can be applied as tissue models for research and screening. However, the lack of biomechanical properties of these tissue constructs has hampered their application to the regeneration of damaged, degenerated or diseased tissue. Having established a cartilage-focussed research laboratory in the University Medical Center Utrecht, I have addressed this biomechanical limitation of hydrogels through the use of hydrogel composites. Specifically, I have pioneered a 3D bio-printing technology that combines accurately printed small diameter thermoplast filaments with cell invasive hydrogels to form strong fibre-reinforced constructs. This, in combination with bioreactor technology, is the key to the generation of larger, complex tissue constructs with cartilage-like biomechanical resilience. With 3D-JOINT I will use my in-depth bio-printing and bioreactor knowledge and experience to develop a multi-phasic 3D-printed biological replacement of the joint.
The world has a significant medical challenge in repairing injured or diseased joints. Joint degeneration and its related pain is a major socio-economic burden that will increase over the next decade and is currently addressed by implanting a metal prosthesis. For the long term, the ideal solution to joint injury is to successfully regenerate rather than replace the damaged cartilage with synthetic implants. Recent advances in key technologies are now bringing this "holy grail" within reach; regenerative approaches, based on cell therapy, are already clinically available albeit only for smaller focal cartilage defects. One of these key technologies is three-dimensional (3D) bio-printing, which provides a greatly controlled placement and organization of living constructs through the layer-by-layer deposition of materials and cells. These tissue constructs can be applied as tissue models for research and screening. However, the lack of biomechanical properties of these tissue constructs has hampered their application to the regeneration of damaged, degenerated or diseased tissue. Having established a cartilage-focussed research laboratory in the University Medical Center Utrecht, I have addressed this biomechanical limitation of hydrogels through the use of hydrogel composites. Specifically, I have pioneered a 3D bio-printing technology that combines accurately printed small diameter thermoplast filaments with cell invasive hydrogels to form strong fibre-reinforced constructs. This, in combination with bioreactor technology, is the key to the generation of larger, complex tissue constructs with cartilage-like biomechanical resilience. With 3D-JOINT I will use my in-depth bio-printing and bioreactor knowledge and experience to develop a multi-phasic 3D-printed biological replacement of the joint.
Project acronym 3D-REPAIR
Project Spatial organization of DNA repair within the nucleus
Researcher (PI) Evanthia Soutoglou
Host Institution (HI) THE UNIVERSITY OF SUSSEX
Summary Faithful repair of double stranded DNA breaks (DSBs) is essential, as they are at the origin of genome instability, chromosomal translocations and cancer. Cells repair DSBs through different pathways, which can be faithful or mutagenic, and the balance between them at a given locus must be tightly regulated to preserve genome integrity. Although, much is known about DSB repair factors, how the choice between pathways is controlled within the nuclear environment is not understood. We have shown that nuclear architecture and non-random genome organization determine the frequency of chromosomal translocations and that pathway choice is dictated by the spatial organization of DNA in the nucleus. Nevertheless, what determines which pathway is activated in response to DSBs at specific genomic locations is not understood. Furthermore, the impact of 3D-genome folding on the kinetics and efficiency of DSB repair is completely unknown. Here we aim to understand how nuclear compartmentalization, chromatin structure and genome organization impact on the efficiency of detection, signaling and repair of DSBs. We will unravel what determines the DNA repair specificity within distinct nuclear compartments using protein tethering, promiscuous biotinylation and quantitative proteomics. We will determine how DNA repair is orchestrated at different heterochromatin structures using a CRISPR/Cas9-based system that allows, for the first time robust induction of DSBs at specific heterochromatin compartments. Finally, we will investigate the role of 3D-genome folding in the kinetics of DNA repair and pathway choice using single nucleotide resolution DSB-mapping coupled to 3D-topological maps. This proposal has significant implications for understanding the mechanisms controlling DNA repair within the nuclear environment and will reveal the regions of the genome that are susceptible to genomic instability and help us understand why certain mutations and translocations are recurrent in cancer
Faithful repair of double stranded DNA breaks (DSBs) is essential, as they are at the origin of genome instability, chromosomal translocations and cancer. Cells repair DSBs through different pathways, which can be faithful or mutagenic, and the balance between them at a given locus must be tightly regulated to preserve genome integrity. Although, much is known about DSB repair factors, how the choice between pathways is controlled within the nuclear environment is not understood. We have shown that nuclear architecture and non-random genome organization determine the frequency of chromosomal translocations and that pathway choice is dictated by the spatial organization of DNA in the nucleus. Nevertheless, what determines which pathway is activated in response to DSBs at specific genomic locations is not understood. Furthermore, the impact of 3D-genome folding on the kinetics and efficiency of DSB repair is completely unknown. Here we aim to understand how nuclear compartmentalization, chromatin structure and genome organization impact on the efficiency of detection, signaling and repair of DSBs. We will unravel what determines the DNA repair specificity within distinct nuclear compartments using protein tethering, promiscuous biotinylation and quantitative proteomics. We will determine how DNA repair is orchestrated at different heterochromatin structures using a CRISPR/Cas9-based system that allows, for the first time robust induction of DSBs at specific heterochromatin compartments. Finally, we will investigate the role of 3D-genome folding in the kinetics of DNA repair and pathway choice using single nucleotide resolution DSB-mapping coupled to 3D-topological maps. This proposal has significant implications for understanding the mechanisms controlling DNA repair within the nuclear environment and will reveal the regions of the genome that are susceptible to genomic instability and help us understand why certain mutations and translocations are recurrent in cancer
Project acronym 3DSPIN
Project 3-Dimensional Maps of the Spinning Nucleon
Researcher (PI) Alessandro Bacchetta
Host Institution (HI) UNIVERSITA DEGLI STUDI DI PAVIA
Call Details Consolidator Grant (CoG), PE2, ERC-2014-CoG
Summary How does the inside of the proton look like? What generates its spin?
3DSPIN will deliver essential information to answer these questions at the frontier of subnuclear physics. At present, we have detailed maps of the distribution of quarks and gluons in the nucleon in 1D (as a function of their momentum in a single direction). We also know that quark spins account for only about 1/3 of the spin of the nucleon. 3DSPIN will lead the way into a new stage of nucleon mapping, explore the distribution of quarks in full 3D momentum space and obtain unprecedented information on orbital angular momentum. Goals 1. extract from experimental data the 3D distribution of quarks (in momentum space), as described by Transverse-Momentum Distributions (TMDs); 2. obtain from TMDs information on quark Orbital Angular Momentum (OAM). Methodology 3DSPIN will implement state-of-the-art fitting procedures to analyze relevant experimental data and extract quark TMDs, similarly to global fits of standard parton distribution functions. Information about quark angular momentum will be obtained through assumptions based on theoretical considerations. The next five years represent an ideal time window to accomplish our goals, thanks to the wealth of expected data from deep-inelastic scattering experiments (COMPASS, Jefferson Lab), hadronic colliders (Fermilab, BNL, LHC), and electron-positron colliders (BELLE, BABAR). The PI has a strong reputation in this field. The group will operate in partnership with the Italian National Institute of Nuclear Physics and in close interaction with leading experts and experimental collaborations worldwide. Impact Mapping the 3D structure of chemical compounds has revolutionized chemistry. Similarly, mapping the 3D structure of the nucleon will have a deep impact on our understanding of the fundamental constituents of matter. We will open new perspectives on the dynamics of quarks and gluons and sharpen our view of high-energy processes involving nucleons.
How does the inside of the proton look like? What generates its spin?
3DSPIN will deliver essential information to answer these questions at the frontier of subnuclear physics. At present, we have detailed maps of the distribution of quarks and gluons in the nucleon in 1D (as a function of their momentum in a single direction). We also know that quark spins account for only about 1/3 of the spin of the nucleon. 3DSPIN will lead the way into a new stage of nucleon mapping, explore the distribution of quarks in full 3D momentum space and obtain unprecedented information on orbital angular momentum. Goals 1. extract from experimental data the 3D distribution of quarks (in momentum space), as described by Transverse-Momentum Distributions (TMDs); 2. obtain from TMDs information on quark Orbital Angular Momentum (OAM). Methodology 3DSPIN will implement state-of-the-art fitting procedures to analyze relevant experimental data and extract quark TMDs, similarly to global fits of standard parton distribution functions. Information about quark angular momentum will be obtained through assumptions based on theoretical considerations. The next five years represent an ideal time window to accomplish our goals, thanks to the wealth of expected data from deep-inelastic scattering experiments (COMPASS, Jefferson Lab), hadronic colliders (Fermilab, BNL, LHC), and electron-positron colliders (BELLE, BABAR). The PI has a strong reputation in this field. The group will operate in partnership with the Italian National Institute of Nuclear Physics and in close interaction with leading experts and experimental collaborations worldwide. Impact Mapping the 3D structure of chemical compounds has revolutionized chemistry. Similarly, mapping the 3D structure of the nucleon will have a deep impact on our understanding of the fundamental constituents of matter. We will open new perspectives on the dynamics of quarks and gluons and sharpen our view of high-energy processes involving nucleons.
Project acronym 3MC
Project 3D Model Catalysts to explore new routes to sustainable fuels
Researcher (PI) Petra Elisabeth De jongh
Host Institution (HI) UNIVERSITEIT UTRECHT
Summary Currently fuels, plastics, and drugs are predominantly manufactured from oil. A transition towards renewable resources critically depends on new catalysts, for instance to convert small molecules (such as solar or biomass derived hydrogen, carbon monoxide, water and carbon dioxide) into more complex ones (such as oxygenates, containing oxygen atoms in their structure). Catalyst development now often depends on trial and error rather than rational design, as the heterogeneity of these composite systems hampers detailed understanding of the role of each of the components. I propose 3D model catalysts as a novel enabling tool to overcome this problem. Their well-defined nature allows unprecedented precision in the variation of structural parameters (morphology, spatial distribution) of the individual components, while at the same time they mimic real catalysts closely enough to allow testing under industrially relevant conditions. Using this approach I will address fundamental questions, such as: * What are the mechanisms (structural, electronic, chemical) by which non-metal promoters influence the functionality of copper-based catalysts? * Which nanoalloys can be formed, how does their composition influence the surface active sites and catalytic functionality under reaction conditions? * Which size and interface effects occur, and how can we use them to tune the actitivity and selectivity towards desired products? Our 3D model catalysts will be assembled from ordered mesoporous silica and carbon support materials and Cu-based promoted and bimetallic nanoparticles. The combination with high resolution characterization and testing under realistic conditions allows detailed insight into the role of the different components; critical for the rational design of novel catalysts for a future more sustainable production of chemicals and fuels from renewable resources.
Currently fuels, plastics, and drugs are predominantly manufactured from oil. A transition towards renewable resources critically depends on new catalysts, for instance to convert small molecules (such as solar or biomass derived hydrogen, carbon monoxide, water and carbon dioxide) into more complex ones (such as oxygenates, containing oxygen atoms in their structure). Catalyst development now often depends on trial and error rather than rational design, as the heterogeneity of these composite systems hampers detailed understanding of the role of each of the components. I propose 3D model catalysts as a novel enabling tool to overcome this problem. Their well-defined nature allows unprecedented precision in the variation of structural parameters (morphology, spatial distribution) of the individual components, while at the same time they mimic real catalysts closely enough to allow testing under industrially relevant conditions. Using this approach I will address fundamental questions, such as: * What are the mechanisms (structural, electronic, chemical) by which non-metal promoters influence the functionality of copper-based catalysts? * Which nanoalloys can be formed, how does their composition influence the surface active sites and catalytic functionality under reaction conditions? * Which size and interface effects occur, and how can we use them to tune the actitivity and selectivity towards desired products? Our 3D model catalysts will be assembled from ordered mesoporous silica and carbon support materials and Cu-based promoted and bimetallic nanoparticles. The combination with high resolution characterization and testing under realistic conditions allows detailed insight into the role of the different components; critical for the rational design of novel catalysts for a future more sustainable production of chemicals and fuels from renewable resources.
Project acronym 4DPHOTON
Project Beyond Light Imaging: High-Rate Single-Photon Detection in Four Dimensions
Researcher (PI) Massimiliano FIORINI
Host Institution (HI) ISTITUTO NAZIONALE DI FISICA NUCLEARE
Summary Goal of the 4DPHOTON project is the development and construction of a photon imaging detector with unprecedented performance. The proposed device will be capable of detecting fluxes of single-photons up to one billion photons per second, over areas of several square centimetres, and will measure - for each photon - position and time simultaneously with resolutions better than ten microns and few tens of picoseconds, respectively. These figures of merit will open many important applications allowing significant advances in particle physics, life sciences or other emerging fields where excellent timing and position resolutions are simultaneously required. Our goal will be achieved thanks to the use of an application-specific integrated circuit in 65 nm complementary metal-oxide-semiconductor (CMOS) technology, that will deliver a timing resolution of few tens of picoseconds at the pixel level, over few hundred thousand individually-active pixel channels, allowing very high rates of photons to be detected, and the corresponding information digitized and transferred to a processing unit. As a result of the 4DPHOTON project we will remove the constraints that many light imaging applications have due to the lack of precise single-photon information on four dimensions (4D): the three spatial coordinates and time simultaneously. In particular, we will prove the performance of this detector in the field of particle physics, performing the reconstruction of Cherenkov photon rings with a timing resolution of ten picoseconds. With its excellent granularity, timing resolution, rate capability and compactness, this detector will represent a new paradigm for the realisation of future Ring Imaging Cherenkov detectors, capable of achieving high efficiency particle identification in environments with very high particle multiplicities, exploiting time-association of the photon hits.
Goal of the 4DPHOTON project is the development and construction of a photon imaging detector with unprecedented performance. The proposed device will be capable of detecting fluxes of single-photons up to one billion photons per second, over areas of several square centimetres, and will measure - for each photon - position and time simultaneously with resolutions better than ten microns and few tens of picoseconds, respectively. These figures of merit will open many important applications allowing significant advances in particle physics, life sciences or other emerging fields where excellent timing and position resolutions are simultaneously required. Our goal will be achieved thanks to the use of an application-specific integrated circuit in 65 nm complementary metal-oxide-semiconductor (CMOS) technology, that will deliver a timing resolution of few tens of picoseconds at the pixel level, over few hundred thousand individually-active pixel channels, allowing very high rates of photons to be detected, and the corresponding information digitized and transferred to a processing unit. As a result of the 4DPHOTON project we will remove the constraints that many light imaging applications have due to the lack of precise single-photon information on four dimensions (4D): the three spatial coordinates and time simultaneously. In particular, we will prove the performance of this detector in the field of particle physics, performing the reconstruction of Cherenkov photon rings with a timing resolution of ten picoseconds. With its excellent granularity, timing resolution, rate capability and compactness, this detector will represent a new paradigm for the realisation of future Ring Imaging Cherenkov detectors, capable of achieving high efficiency particle identification in environments with very high particle multiplicities, exploiting time-association of the photon hits.
Project acronym 9 SALT
Project Reassessing Ninth Century Philosophy. A Synchronic Approach to the Logical Traditions
Researcher (PI) Christophe Florian Erismann
Host Institution (HI) UNIVERSITAT WIEN
Call Details Consolidator Grant (CoG), SH5, ERC-2014-CoG
Summary This project aims at a better understanding of the philosophical richness of ninth century thought using the unprecedented and highly innovative method of the synchronic approach. The hypothesis directing this synchronic approach is that studying together in parallel the four main philosophical traditions of the century – i.e. Latin, Greek, Syriac and Arabic – will bring results that the traditional enquiry limited to one tradition alone can never reach. This implies pioneering a new methodology to overcome the compartmentalization of research which prevails nowadays. Using this method is only possible because the four conditions of applicability – comparable intellectual environment, common text corpus, similar methodological perspective, commensurable problems – are fulfilled. The ninth century, a time of cultural renewal in the Carolingian, Byzantine and Abbasid empires, possesses the remarkable characteristic – which ensures commensurability – that the same texts, namely the writings of Aristotelian logic (mainly Porphyry's Isagoge and Aristotle's Categories) were read and commented upon in Latin, Greek, Syriac and Arabic alike. Logic is fundamental to philosophical enquiry. The contested question is the human capacity to rationalise, analyse and describe the sensible reality, to understand the ontological structure of the world, and to define the types of entities which exist. The use of this unprecedented synchronic approach will allow us a deeper understanding of the positions, a clear identification of the a priori postulates of the philosophical debates, and a critical evaluation of the arguments used. It provides a unique opportunity to compare the different traditions and highlight the heritage which is common, to stress the specificities of each tradition when tackling philosophical issues and to discover the doctrinal results triggered by their mutual interactions, be they constructive (scholarly exchanges) or polemic (religious controversies).
This project aims at a better understanding of the philosophical richness of ninth century thought using the unprecedented and highly innovative method of the synchronic approach. The hypothesis directing this synchronic approach is that studying together in parallel the four main philosophical traditions of the century – i.e. Latin, Greek, Syriac and Arabic – will bring results that the traditional enquiry limited to one tradition alone can never reach. This implies pioneering a new methodology to overcome the compartmentalization of research which prevails nowadays. Using this method is only possible because the four conditions of applicability – comparable intellectual environment, common text corpus, similar methodological perspective, commensurable problems – are fulfilled. The ninth century, a time of cultural renewal in the Carolingian, Byzantine and Abbasid empires, possesses the remarkable characteristic – which ensures commensurability – that the same texts, namely the writings of Aristotelian logic (mainly Porphyry's Isagoge and Aristotle's Categories) were read and commented upon in Latin, Greek, Syriac and Arabic alike. Logic is fundamental to philosophical enquiry. The contested question is the human capacity to rationalise, analyse and describe the sensible reality, to understand the ontological structure of the world, and to define the types of entities which exist. The use of this unprecedented synchronic approach will allow us a deeper understanding of the positions, a clear identification of the a priori postulates of the philosophical debates, and a critical evaluation of the arguments used. It provides a unique opportunity to compare the different traditions and highlight the heritage which is common, to stress the specificities of each tradition when tackling philosophical issues and to discover the doctrinal results triggered by their mutual interactions, be they constructive (scholarly exchanges) or polemic (religious controversies).
Project acronym A-DIET
Project Metabolomics based biomarkers of dietary intake- new tools for nutrition research
Researcher (PI) Lorraine Brennan
Host Institution (HI) UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Country Ireland
Summary In todays advanced technological world, we can track the exact movement of individuals, analyse their genetic makeup and predict predisposition to certain diseases. However, we are unable to accurately assess an individual's dietary intake. This is without a doubt one of the main stumbling blocks in assessing the link between diet and disease/health. The present proposal (A-DIET) will address this issue with the overarching objective to develop novel strategies for assessment of dietary intake. Using approaches to (1) identify biomarkers of specific foods (2) classify people into dietary patterns (nutritypes) and (3) develop a tool for integration of dietary and biomarker data, A-DIET has the potential to dramatically enhance our ability to accurately assess dietary intake. The ultimate output from A-DIET will be a dietary assessment tool which can be used to obtain an accurate assessment of dietary intake by combining dietary and biomarker data which in turn will allow investigations into relationships between diet, health and disease. New biomarkers of specific foods will be identified and validated using intervention studies and metabolomic analyses. Methods will be developed to classify individuals into dietary patterns based on biomarker/metabolomic profiles thus demonstrating the novel concept of nutritypes. Strategies for integration of dietary and biomarker data will be developed and translated into a tool that will be made available to the wider scientific community. Advances made in A-DIET will enable nutrition epidemiologist's to properly examine the relationship between diet and disease and develop clear public health messages with regard to diet and health. Additionally results from A-DIET will allow researchers to accurately assess people's diet and implement health promotion strategies and enable dieticians in a clinical environment to assess compliance to therapeutic diets such as adherence to a high fibre diet or a gluten free diet.
In todays advanced technological world, we can track the exact movement of individuals, analyse their genetic makeup and predict predisposition to certain diseases. However, we are unable to accurately assess an individual's dietary intake. This is without a doubt one of the main stumbling blocks in assessing the link between diet and disease/health. The present proposal (A-DIET) will address this issue with the overarching objective to develop novel strategies for assessment of dietary intake. Using approaches to (1) identify biomarkers of specific foods (2) classify people into dietary patterns (nutritypes) and (3) develop a tool for integration of dietary and biomarker data, A-DIET has the potential to dramatically enhance our ability to accurately assess dietary intake. The ultimate output from A-DIET will be a dietary assessment tool which can be used to obtain an accurate assessment of dietary intake by combining dietary and biomarker data which in turn will allow investigations into relationships between diet, health and disease. New biomarkers of specific foods will be identified and validated using intervention studies and metabolomic analyses. Methods will be developed to classify individuals into dietary patterns based on biomarker/metabolomic profiles thus demonstrating the novel concept of nutritypes. Strategies for integration of dietary and biomarker data will be developed and translated into a tool that will be made available to the wider scientific community. Advances made in A-DIET will enable nutrition epidemiologist's to properly examine the relationship between diet and disease and develop clear public health messages with regard to diet and health. Additionally results from A-DIET will allow researchers to accurately assess people's diet and implement health promotion strategies and enable dieticians in a clinical environment to assess compliance to therapeutic diets such as adherence to a high fibre diet or a gluten free diet.
Project acronym A-FRO
Project Actively Frozen - contextual modulation of freezing and its neuronal basis
Researcher (PI) Marta de Aragao Pacheco Moita
Host Institution (HI) FUNDACAO D. ANNA SOMMER CHAMPALIMAUD E DR. CARLOS MONTEZ CHAMPALIMAUD
Summary When faced with a threat, an animal must decide whether to freeze, reducing its chances of being noticed, or to flee to the safety of a refuge. Animals from fish to primates choose between these two alternatives when confronted by an attacking predator, a choice that largely depends on the context in which the threat occurs. Recent work has made strides identifying the pre-motor circuits, and their inputs, which control freezing behavior in rodents, but how contextual information is integrated to guide this choice is still far from understood. We recently found that fruit flies in response to visual looming stimuli, simulating a large object on collision course, make rapid freeze/flee choices that depend on the social and spatial environment, and the fly's internal state. Further, identification of looming detector neurons was recently reported and we identified the descending command neurons, DNp09, responsible for freezing in the fly. Knowing the sensory input and descending output for looming-evoked freezing, two environmental factors that modulate its expression, and using a genetically tractable system affording the use of large sample sizes, places us in an unique position to understand how a information about a threat is integrated with cues from the environment to guide the choice of whether to freeze (our goal). To assess how social information impinges on the circuit for freezing, we will examine the sensory inputs and neuromodulators that mediate this process, mapping their connections to DNp09 neurons (Aim 1). We ask whether learning is required for the spatial modulation of freezing, which cues flies are using to discriminate different places and which brain circuits mediate this process (Aim 2). Finally, we will study how activity of DNp09 neurons drives freezing (Aim 3). This project will provide a comprehensive understanding of the mechanism of freezing and its modulation by the environment, from single neurons to behaviour.
When faced with a threat, an animal must decide whether to freeze, reducing its chances of being noticed, or to flee to the safety of a refuge. Animals from fish to primates choose between these two alternatives when confronted by an attacking predator, a choice that largely depends on the context in which the threat occurs. Recent work has made strides identifying the pre-motor circuits, and their inputs, which control freezing behavior in rodents, but how contextual information is integrated to guide this choice is still far from understood. We recently found that fruit flies in response to visual looming stimuli, simulating a large object on collision course, make rapid freeze/flee choices that depend on the social and spatial environment, and the fly's internal state. Further, identification of looming detector neurons was recently reported and we identified the descending command neurons, DNp09, responsible for freezing in the fly. Knowing the sensory input and descending output for looming-evoked freezing, two environmental factors that modulate its expression, and using a genetically tractable system affording the use of large sample sizes, places us in an unique position to understand how a information about a threat is integrated with cues from the environment to guide the choice of whether to freeze (our goal). To assess how social information impinges on the circuit for freezing, we will examine the sensory inputs and neuromodulators that mediate this process, mapping their connections to DNp09 neurons (Aim 1). We ask whether learning is required for the spatial modulation of freezing, which cues flies are using to discriminate different places and which brain circuits mediate this process (Aim 2). Finally, we will study how activity of DNp09 neurons drives freezing (Aim 3). This project will provide a comprehensive understanding of the mechanism of freezing and its modulation by the environment, from single neurons to behaviour.
Project acronym ACoolTouch
Project Neural mechanisms of multisensory perceptual binding
Researcher (PI) James Francis Alexander Poulet
Host Institution (HI) MAX DELBRUECK CENTRUM FUER MOLEKULARE MEDIZIN IN DER HELMHOLTZ-GEMEINSCHAFT (MDC)
Summary Sensory perception involves the discrimination and binding of multiple modalities of sensory input. This is especially evident in the somatosensory system where different modalities of sensory input, including thermal and mechanosensory, are combined to generate a unified percept. The neural mechanisms of multisensory binding are unknown, in part because sensory perception is typically studied within a single modality in a single brain region. I propose a multi-level approach to investigate thermo-tactile processing in the mouse forepaw system from the primary sensory afferent neurons to thalamo-cortical circuits and behaviour. The mouse forepaw system is the ideal system to investigate multisensory binding as the sensory afferent neurons are well investigated, cell type-specific lines are available, in vivo optogenetic manipulation is possible both in sensory afferent neurons and central circuits and we have developed high-resolution somatosensory perception behaviours. We have previously shown that mouse primary somatosensory forepaw cortical neurons respond to both tactile and thermal stimuli and are required for non-noxious cooling perception. With multimodal neurons how, then, is it possible to both discriminate and bind thermal and tactile stimuli? I propose 3 objectives to address this question. We will first, perform functional mapping of the thermal and tactile pathways to cortex; second, investigate the neural mechanisms of thermo-tactile discrimination in behaving mice; and third, compare neural processing during two thermo-tactile binding tasks, the first using passively applied stimuli, and the second, active manipulation of thermal objects. At each stage we will perform cell type-specific neural recordings and causal optogenetic manipulations in awake and behaving mice. Our multi-level approach will provide a comprehensive investigation into how the brain performs multisensory perceptual binding: a fundamental yet unsolved problem in neuroscience.
Sensory perception involves the discrimination and binding of multiple modalities of sensory input. This is especially evident in the somatosensory system where different modalities of sensory input, including thermal and mechanosensory, are combined to generate a unified percept. The neural mechanisms of multisensory binding are unknown, in part because sensory perception is typically studied within a single modality in a single brain region. I propose a multi-level approach to investigate thermo-tactile processing in the mouse forepaw system from the primary sensory afferent neurons to thalamo-cortical circuits and behaviour. The mouse forepaw system is the ideal system to investigate multisensory binding as the sensory afferent neurons are well investigated, cell type-specific lines are available, in vivo optogenetic manipulation is possible both in sensory afferent neurons and central circuits and we have developed high-resolution somatosensory perception behaviours. We have previously shown that mouse primary somatosensory forepaw cortical neurons respond to both tactile and thermal stimuli and are required for non-noxious cooling perception. With multimodal neurons how, then, is it possible to both discriminate and bind thermal and tactile stimuli? I propose 3 objectives to address this question. We will first, perform functional mapping of the thermal and tactile pathways to cortex; second, investigate the neural mechanisms of thermo-tactile discrimination in behaving mice; and third, compare neural processing during two thermo-tactile binding tasks, the first using passively applied stimuli, and the second, active manipulation of thermal objects. At each stage we will perform cell type-specific neural recordings and causal optogenetic manipulations in awake and behaving mice. Our multi-level approach will provide a comprehensive investigation into how the brain performs multisensory perceptual binding: a fundamental yet unsolved problem in neuroscience.
Project acronym ActionContraThreat
Project Action selection under threat: the complex control of human defense
Researcher (PI) Dominik BACH
Host Institution (HI) UNIVERSITY COLLEGE LONDON
Summary Run away, sidestep, duck-and-cover, watch: when under threat, humans immediately choreograph a large repertoire of defensive actions. Understanding action-selection under threat is important for anybody wanting to explain why anxiety disorders imply some of these behaviours in harmless situations. Current concepts of human defensive behaviour are largely derived from rodent research and focus on a small number of broad, cross-species, action tendencies. This is likely to underestimate the complexity of the underlying action-selection mechanisms. This research programme will take decisive steps to understand these psychological mechanisms and elucidate their neural implementation. To elicit threat-related action in the laboratory, I will use virtual reality computer games with full body motion, and track actions with motion-capture technology. Based on a cognitive-computational framework, I will systematically characterise the space of actions under threat, investigate the psychological mechanisms by which actions are selected in different scenarios, and describe them with computational algorithms that allow quantitative predictions. To independently verify their neural implementation, I will use wearable magnetoencephalography (MEG) in freely moving subjects. This proposal fills a lacuna between defence system concepts based on rodent research, emotion psychology, and clinical accounts of anxiety disorders. By combining a stringent experimental approach with the formalism of cognitive-computational psychology, it furnishes a unique opportunity to understand the mechanisms of action-selection under threat, and how these are distinct from more general-purpose action-selection systems. Beyond its immediate scope, the proposal has a potential to lead to a better understanding of anxiety disorders, and to pave the way towards improved diagnostics and therapies.
Run away, sidestep, duck-and-cover, watch: when under threat, humans immediately choreograph a large repertoire of defensive actions. Understanding action-selection under threat is important for anybody wanting to explain why anxiety disorders imply some of these behaviours in harmless situations. Current concepts of human defensive behaviour are largely derived from rodent research and focus on a small number of broad, cross-species, action tendencies. This is likely to underestimate the complexity of the underlying action-selection mechanisms. This research programme will take decisive steps to understand these psychological mechanisms and elucidate their neural implementation. To elicit threat-related action in the laboratory, I will use virtual reality computer games with full body motion, and track actions with motion-capture technology. Based on a cognitive-computational framework, I will systematically characterise the space of actions under threat, investigate the psychological mechanisms by which actions are selected in different scenarios, and describe them with computational algorithms that allow quantitative predictions. To independently verify their neural implementation, I will use wearable magnetoencephalography (MEG) in freely moving subjects. This proposal fills a lacuna between defence system concepts based on rodent research, emotion psychology, and clinical accounts of anxiety disorders. By combining a stringent experimental approach with the formalism of cognitive-computational psychology, it furnishes a unique opportunity to understand the mechanisms of action-selection under threat, and how these are distinct from more general-purpose action-selection systems. Beyond its immediate scope, the proposal has a potential to lead to a better understanding of anxiety disorders, and to pave the way towards improved diagnostics and therapies.


His And Her Christmas Pajamas Deals Price Comparison. Halloween Store. Incredible selection of Halloween Costumes, Halloween Decorations and more!
Fun gift for a fun couple in love! Perfect for newlyweds, anniversary or birthday gift for that special someone. Choose wine or beer graphic with matching Cheers! expression for a fun lounge set to relax in with your favorite beverage! Short-sleeve shirt is 100% Cotton Short-Sleeve crewneck Shirt; combed for softness, in black; print is white. Pants are Black/White Plaid Print, 100% Double Brushed Cotton Flannel, Soft Covered Elastic wast, Pockets, Unisex Sizing. ** All Shirt-Pant sets sold SEPARATELY - Select design and size details and add EACH set to your shopping cart separately. Note each shirt and pant set is $48.00 and you must purchase separately for each person.
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The conference's "Employer's brand development in modern conditions" insights
Author: Olena Skyba, the WUO EBA HR Committee Chair, Kredobank HR Director The conferences Employers brand development in modern conditions, which took place on October 21, 2021 at the EBA WUO initiative with the partners support of Kredobank, Ancor Ukraine and Robota.ua insights. The pandemic and its social-economic consequences have greatly affected our lives for the last 2 years. On the one hand, this challenge has forced the companies to start projects on optimization, efficiency increase, remote work and digital processes set up, on the other hand — it has highlighted the human relations and man himself importance, having strengthened the trend of business humanization. This means that: People are not indifferent to the place where they work, to whom, which values are shared by the companys staff, its management, For people, its important whom they work with, who surrounds them and how these people treat their individuality, and also feel that they are understood, valued, cared of, For people, its important what and for what they do anything, to feel pride about their activities results, to feel that they are investing their time correctly, achieving an inspiring aim, to feel autonomy while doing work. At the same time, it is easier for people to change jobs. If at the beginning of the pandemic the pace of recruitment fell sharply, everyone was waiting to return to normal, now there is a return to the pace of 2019. The competition is high, especially for professionals and high-potential workers who can think and learn quickly, easily adapt as to work and team, and to constant change. Therefore, in order to stay in trend and to be competitive, it is important for companies to: Develop the constant feedback culture in the company, using all possible formats, learn to use the employee suffrage in decision-making, provide support and timely change what has ceased to be effective and lost relevance, explain difficult decisions, clarify prospects, answer painful questions, Ensure transparent conditions of remuneration and agreements implementation, take into account the individual priorities and characteristics of key employees, Pay more, in case of extra results, do not stop thanking people for their contribution to business and team development, Review EVP and adapt, target communications to different target groups separately, Take care of society and the environment, involving the employees, Encourage the development, initiative and innovation of employees, Take care of peoples physical and mental health, help in difficult life situations, reduce stress at work, including due to transparent communications and reduction of uncertainty, To unite people using an important aim that will form a sense of unity and affiliation to something bigger and more significant. Personally, I like the importance of staying human in relation to employees and each other to be in trend. Be yourself and respect the right of others to be themselves! May your brand become stronger and your people happier! To obtain the access to the full video conference, leave a request. Please note that video for non members of the Association is being charged.
Author: Olena Skyba, the WUO EBA HR Committee Chair, Kredobank HR Director
The conference's "Employer's brand development in modern conditions", which took place on October 21, 2021 at the EBA WUO initiative with the partners' support of Kredobank, Ancor Ukraine and Robota.ua insights.
The pandemic and its social-economic consequences have greatly affected our lives for the last 2 years. On the one hand, this challenge has forced the companies to start projects on optimization, efficiency increase, remote work and digital processes set up, on the other hand — it has highlighted the human relations and man himself importance, having strengthened the trend of business' humanization.
This means that:
People are not indifferent to the place where they work, to whom, which values are shared by the company's staff, its management,
For people, it's important whom they work with, who surrounds them and how these people treat their individuality, and also feel that they are understood, valued, cared of,
For people, it's important what and for what they do anything, to feel pride about their activities results, to feel that they are investing their time correctly, achieving an inspiring aim, to feel autonomy while doing work.
At the same time, it is easier for people to change jobs. If at the beginning of the pandemic the pace of recruitment fell sharply, everyone was waiting to return to normal, now there is a return to the pace of 2019. The competition is high, especially for professionals and high-potential workers who can think and learn quickly, easily adapt as to work and team, and to constant change.
Therefore, in order to stay in trend and to be competitive, it is important for companies to:
Develop the constant feedback culture in the company, using all possible formats, learn to use the employee suffrage in decision-making, provide support and timely change what has ceased to be effective and lost relevance, explain difficult decisions, clarify prospects, answer painful questions,
Ensure transparent conditions of remuneration and agreements implementation, take into account the individual priorities and characteristics of key employees,
Pay more, in case of extra results, do not stop thanking people for their contribution to business and team development,
Review EVP and adapt, target communications to different target groups separately,
Take care of society and the environment, involving the employees,
Encourage the development, initiative and innovation of employees,
Take care of people's physical and mental health, help in difficult life situations, reduce stress at work, including due to transparent communications and reduction of uncertainty,
To unite people using an important aim that will form a sense of unity and affiliation to something bigger and more significant.
Personally, I like the importance of staying human in relation to employees and each other to be in trend. Be yourself and respect the right of others to be themselves!
May your brand become stronger and your people happier!
To obtain the access to the full video conference, leave a request. Please note that video for non members of the Association is being charged.


"TODAY, THEY HAVE SOMEHOW GROWN AFRAID OF THE JEWS"
This photograph shows items used by religious Jews in prayer on display in the Auschwitz-Birkenau Museum. Physical resistance was not a realistic option for most people during the Holocaust. Nonetheless, many Jews found ways of asserting basic human dignity. One survivor, Yoysef Vaynberg, recalled what happened in Auschwitz-Birkenau on Yom Kippur, the holiest day in the Jewish calendar, in 1944. Kol Nidre is a ceremony performed as part of the Yom Kippur service.
After the evening roll call, we go to Kol Nidre.
For a long time already we have been promising each other to observe the Kol Nidre service this year. A Jewish block elder has allowed us to pray in his block. Someone has brought a tallis [prayer shawl] from the clothing warehouse. The seriousness of the moment is felt in the camp. It seems that the entire world is preparing for Kol Nidre.
In the morning the entire sky was clouded over. At midday the cloud rose and it rained. The sun hid somewhere behind the clouds. Heaven wept for an entire afternoon. Now, before Kol Nidre, it calmed itself a bit: the rain stopped. The world around lies desolate. The sun feels guilty and doesn't dare to show its face.
From every block, people assembled at the barrack of the Jewish block elder. People stretch out on the pallets, stand pressed next to one another. Everyone who feels a Jewish heart beating inside has come, even the block elders and kapos [prisoners who were chosen by the SS to oversee other prisoners]. They are always the grand aristocrats. Now they are standing among the ordinary 'prisoners'. They are possessed by dread. Even the German block elders and kapos, those terrible murderers are silent. They avoid the barrack, moving in a large semicircle around it. Today, they have somehow grown afraid of the Jews.
Some have seen this preservation of religious practices in the most unimaginable of circumstances as a form of what has been termed 'spiritual resistance', a defiant defence of the Jewish traditions which the Nazis were seeking to destroy.


Posted November 8th, 2018 by Sage & filed under Media, Training and Racing, Yoga.
Read: Are Your Hip Muscles out of Balance?
Posted April 8th, 2014 by Sage & filed under Coaching, Media, Training and Racing, Yoga.
Posted August 15th, 2011 by Sage & filed under Active Yogi, Recovery, Training and Racing, Yoga.
My latest post for Active Yogi, my blog at Yoga Journal, is on legs up the wall, my go-to recovery pose. Do you have a favorite?
Posted August 6th, 2011 by Sage & filed under Media, Training and Racing, Yoga.
Posted July 19th, 2011 by Sage & filed under Active Yogi, Media, Yoga.
My newest Active Yogi blog post for Yoga Journal looks at pigeon pose from many directions. When I opened this post to link to it, I remembered having written on the subject before, as part of my video series for Competitor.com. See that link here.
Posted October 15th, 2009 by Sage & filed under Media, Training and Racing, Yoga.


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Beauticians confess they have a love-hate relationship with their tools. Like other traditional professions, finding the instruments matching perfectly with your requirements is imperative. If you cannot feel comfortable in handling the scissor, obviously your job suffers. Hairdressing is a highly critical work where one cannot afford to mistake. You cannot grow back one's hair! You can only shape it by cutting. In this one-way route, you must be able to create the perfect magical frame surrounding her face. The beautician needs to understand the precise requirements of the client. The Tondeo scissors help greatly in meeting her expectations. You need to find the scissors you would love to use.
In the uni-directional hair-cutting avenue, you cannot afford to be late even. You have to work fast in shaping the head. Experts would continue conversing with the client. Talking actually helps to understand the hairstyling requirements. The secret of good styling is to shape the hair in the exact way your client wants. She would talk about many things. You need to interpret the uniqueness of her personality. Haircutting is a major occasion for women. A girl would visit the salon only when she is ready to take a major decision of her life. Hairstyling is a largely symbolic beauty regime. The framing of the hair alters her appearance! She would not choose a new appearance unless there are strong reasons to it. She would talk about it. Follow her string of thoughts in interpreting the best style giving a face to her new life.
The German-made Tondeo scissors are extremely lightweight. Their attractive design has a vintage feel. The German service has been supplying scissors to the world for more than 75 years. They retain the original authenticity in design and performance. It is quite thrilling to think the same scissor styles used in fashion salons of 1940 Paris are still available. The company made it all possible. Most beautician training schools across the world ensure keeping the Tondeo scissors at their training classes. The product diversity is very useful in growing your scissor collection.
The design aspect makes collectibles of these traditional German engineering specimens. Their amazing utility is evident from the fact that you would not hear much even when you use them furtively. The company makes them specifically for fast use. The ergonomic design snugly adjusts your fingers in a strong grip. They are precision sharp and you need to apply the lightest pressure to work them. The range of scissors allows you to handle any kind of hair issue. Whether you have a fizzy head or a straight one, get the Tondeo scissors in action to create the layers you require.
These appliances actually encourage you in giving the best at styling. Having the German-made Tondeo scissors is a sign of utmost professionalism in hairstyling. The tool set of the top professional is incomplete without a collection of these essential instruments. Find a reliable service offering the scissors at best prices. Inquire whether they have special offers and discounts.


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Q: Group and export to pdf based on an attribute field using DDP - Python Script Using Python, I want to export a map series. Each series needs to be grouped based on an attribute field which is not being used in DDP settings (page num, page index etc...).
For example: my shapefile RoadName.shp has a field called RoadType.  Now when I ask my DDP to export maps, it should group diff RoadTypes into one single (multipage) pdf.
Any quick solution please?

A: This code should achieve what you have asked (not tested):
import arcpy
import os

mxd = arcpy.mapping.MapDocument(r"C:\temp\mymap.mxd")
output_folder = r"C:\temp"
ddp_lyr = mxd.dataDrivenPages.indexLayer
pdf_field = "RoadType"

#Get a list of unique values to group by
pdf_list = set([row[0] for row in arcpy.da.SearchCursor(ddp_lyr,pdf_field)])

for pdfName in pdf_list:
    finalPdf = arcpy.mapping.PDFDocumentCreate(os.path.join(output_folder,pdfName + ".pdf"))
    for i in range(1, mxd.dataDrivenPages.pageCount + 1):
        mxd.dataDrivenPages.currentPageID = i
        cur = str(mxd.dataDrivenPages.pageRow.getValue(pdf_field))
        if cur == pdfName:
            tempPdf = os.path.join(output_folder,"temp_" + cur + str(i) + ".pdf")
            arcpy.mapping.ExportToPDF(mxd, tempPdf)
            finalPdf.appendPages(tempPdf)
            os.remove(tempPdf)
    finalPdf.saveAndClose()
    del finalPdf, tempPdf

The code is not optimised, so it loops over all the data driven pages for each unique value in the list. With some rearranging, it may be better to first export all the pdfs, then check the filenames for the unique value and group them accordingly.

A: Here's a bit of psuedo code and a bit of code rearranged from another stack exchange answer:
roadtypes = list of the unique values in RoadType field
field = "RoadType"
#Loop through the road types
for type in roadtypes:
    queryStr = str("{0}" + " = " + '{1}').format(field, type)

    #Specify the MXD project (CURRENT), dataframe (Layers)
    mxd = arcpy.mapping.MapDocument("CURRENT")
    df = arcpy.mapping.ListDataFrames(mxd, "Layers")[0]

    #Apply defintion query to specified layer group (Test)
    for lyr in arcpy.mapping.ListLayers(mxd, "Test", df)[0]:
        if lyr.supports("DEFINITIONQUERY"):
            lyr.definitionQuery = queryStr
    arcpy.RefreshActiveView()
    #Export map series
    #arcpy.exportToPDF()

redpajama_set_name: RedPajamaStackExchange


communication so can be used over longer distances.
see a common clock line is required between the transmitter & receiver.
where messages are generated at irregular intervals.
is the same then receiver will receive the correct data.
knows that the data is being transmitted.
1 then it assumes error in communication & then dumps the data.
circuit's special driver circuit are required to convert the logic levels.
1.3V, a typical hysterics of 0.5V and can operate up to + 30V input.
1. Suitable for all RS232 communications.
2. +12V power supplies required.
Section of computers, peripherals, and modems).
3. + or - 5V to + or - 10V dual charge pump voltage converter.
high forming the unused transistor output low.
5KΩ and Vcc = + or - 9 v.
and can be short circuited to ground indefinitely.
0.5V of hysterisis to improve noise rejection.
input is floating or driven between +0.8V and –30V.