The Bulgarian Voices Angelite (also known as The Bulgarian Women's Choir Angelite or simply Angelite) is among the most renowned ensembles in the categories of Balkan and Bulgarian folk music. This women's choir has performed practically everywhere: on the Red Square in Moscow, at the award ceremony of the Nobel Peace Prize in Stockholm and at the Vatican. The cryptic, mysterious, perhaps even somewhat mystical quality that characterises the choir's sound arises primarily from the singers' preference for a second voice which is maintained a slight interval to the leading melody. This results in a kind of nearly-dissonant friction as well as a great radiant power, as of an unfolded colour spectrum: The singers' voices fan out into a colourfully glowing sound palette that plays with overtone resonance. As a result, Angelite offers something that goes far beyond common folk music: It is the artful and skilful enhancement of traditional elements to form a style which - despite its a great affinity with the folklore of Bulgaria - can be considered a category of its own. On the one hand it possesses an authentic sound, i.e. preserves the heritage; on the other hand it has advanced into the sphere of art music. See also the collaborations: The Bulgarian Voices Angelite & Huun-Huur-Tu and The Bulgarian Voices Angelite & Moscow Art Trio with Huun-Huur-Tu. Read more on Last.fm. User-contributed text is available under the Creative Commons By-SA License; additional terms may apply.


text:
Icon of the City
By John Tauranac
The Empire State Building is enshrined in New York City's Art Deco firmament, but it is hardly an Art Deco extravaganza. It is more a functionalist's ideal than an Art Deco fantasy.
The Empire State Building's design was one of the city's most elegant of skyscraper profiles. It is essentially all tower, yet it worked within the confines of the zoning law of 1916, which mandated that a building could not rise straight up from the building line and go as high as technologically possible on the bulk of its plot. A building could rise straight up from the building line a multiple of the width of the street it faced, then setbacks stacked upon each other could rise in reduced girth. A tower could rise straight up on 25 percent of the land.
Instead of the lower floors climbing ten or eleven stories before having a setback, the Empire State Building had only a five-story base covering the plot. At the sixth floor was a major setback—60 feet from Fifth Avenue and 20 and 30 feet from
The entrance to the Empire State Building. Photo: Meghan Weatherby
the side streets. From this low base there rose a tower that in diminishing jetés soared majestically to the eighty-fifth floor. It was big, it was bold, it was beautiful.
The harmony of design, with all the elements balanced in true classical form, is sheer elegance. At every stage, horizontally and vertically, there is a beginning, middle, and end.
The classical truths of the building are self-evident. The subtler attributes are not. On the base is stone that has been scored to make the building look stouter, and although the stone is the same limestone as the rest of the building, it adds coloration by virtue of the shadows cast.
The decision to use stone for the facing of the entire building was not based on aesthetics alone. Finished brick is expensive to set, and time consuming. Limestone has a luminosity to it, and it comes with other virtues—it is not as hard as granite, hence easier to turn into ashlar, the finished stone used for the facing, and it is more durable than marble.
The façades were designed without cornices and other architectural features, so there was an uninterrupted flow to the building. The bays conveyed depth, providing a play of light and shadow, with the added benefit of creating a floor plan with more desirable corner offices than a four-cornered tower.
Walls at the setbacks were gently tapered, and the top five floors were chamfered, their corners cut away and softened so that the building reached a logical denouement.
Windows on the lower five floors were installed in the conventional manner. Above, however, the windows were flush with the façade as if the outer wall were a wrapping instead of pretending to be load bearing.
The grandest crown that a building ever wore. Photo: Richard Berenholtz
Typical of Richmond H. Shreve, of the architectural firm of Shreve, Lamb & Harmon, the plan was not purely aesthetic. The window frames were set into the openings so that the frames covered the edges of the flanking stone and were flush with the spandrels. It translated into stone whose edges did not have to be finished, reducing the cost of the stonework and simplifying installation. And the spandrels themselves were prefabricated slabs of cast aluminum decorated with facing pairs of stylized lightning bolts set in a chevron-like pattern in a classic Art Deco geometric form. They only had to be set in place.
Chrome-nickel steel was used for the mullions, the vertical bands separating the windows that would run from the sixth to the eighty-fifth stories. In addition to contributing to the upward sweep of the façade,
the mullions were a critical element in the ease of construction. Like window frames, they too covered the joins. Topping these vertical steel bands were fanlike motifs, Art Deco responses to terminating features that some critics interpreted as sunbursts, others as anthemions.
The great ribbons of windows and spandrels reached for the sky, their soaring quality enhanced by the vertical strips of chrome-nickel steel that flanked them.
But the 102-story building that we know today was not the building as planned. In the fall of 1929, the anticipated height of the Empire State Building was 80 stories, or 1,000 feet. The Otis Elevator Company said that cables any longer than 1,000 feet might just collapse under their own weight, and direct service to the eightieth floor, with its planned observatory, was an important feature. The height of 1,000 feet would also assure the building the world's tallest laurels. The impetus to build a taller building only came when the Chrysler Building rose to a height of 1,048 feet.
Al Smith, the former governor of New York State and presidential candidate, was the president of the Empire State Building, and John J. Raskob, the former chief financial officer of General Motors, was the moneyman. Raskob could not allow the upstart automaker Walter Chrysler to best him. A five-story "penthouse" would be added to the building, taking it to 85 stories, or 1,050 feet, which beat the Chrysler Building by two feet.
But Smith and Raskob feared that another developer could easily come along and do to them what they had done to Chrysler. They were going to need a taller building.
The plan that evolved was perhaps the looniest building scheme since the Tower of Babel. A 200-foot dirigible mooring mast would be erected atop the building. The idea was that a dirigible would be moored from its bow to the top of the mast. A gangplank would drop down from the dirigible, and passengers would blithely walk between the airship and a platform ringing the mast, 1,250 feet in the air.
By the fall of 1931, six months after the building had opened, the plan was quietly dropped. However, the mooring mast was not a total failure. It made the building the world's tallest until the first of the World Trade Towers opened in 1972, and it gave the building one of the grandest crowns that ever a building wore, creating an instant landmark on the city's skyline.
John Tauranac writes on New York's architectural history. He teaches the subject at the NYU School of Professional Studies, and he designs maps. He is the author of The Empire State Building: The Making of a Landmark (reprinted by Cornell University Press), the three editions of New York From the Air, with the great aerial photographer Yann Arthus-Bertrand (Harry Abrams), The View From the 86th Floor (Tauranac Press), and the creator of Manhattan Block By Block: A Street Atlas.
Article originally published in the Art Deco New York journal, Vol. 1, Issue 1, Spring 2016. View a digital version of the full journal here.

redpajama_set_name: RedPajamaCommonCrawl


as I come back to Berlin (Germany), I plan another Xojo developer meeting around 5th/8th June.
Sadly someone sabotaged the voting.
Please contact me directly and let me know if you prefer Tuesday, Friday or Saturday.
@ChristianSchmitz Sadly someone sabotaged the voting.
Anyone can edit all entries unless people voted with login.
@ChristianSchmitz Anyone can edit all entries unless people voted with login.
Looks like we have 8 people for that date.


text:
Home Unlabelled Coolpad Launched Cool 1 Dual in India
Coolpad Launched Cool 1 Dual in India
GADGETS AND INNOVATIONS December 29, 2016
Coolpad the famous Chinese Smartphone manufacturer has launched its new device Cool1 Dual in India on December 28th, 2016, in an event in New Delhi, India. This Device is the result of the collaboration between the two Chinese brand names first is LeEco and the second is Coolpad. Cool 1 is the first Smartphone from the Cool series which brings together software and hardware strength of LeEco and Coolpad.
Cool 1 comes with a 5.5 inches 1080p Full High Definition display with 401PPI pixels resolution which provides a wide viewing experience and better image quality. It has a strong build quality with metallic body that gives Cool 1 a superior and premium look. This Smartphone equips a 13 Megapixels Dual Primary Camera which has Dual-tone Dual LED flash support. For power section it packs a 4000mAh Lithium ion polymer, non-removable battery.
While addressing the media at the launch Coolpad Group, Global CEO, Mr.James Du has said, "India is among the top performing markets for Coolpad where we have sold close to 3 million units. The response we have got from our consumer over the years with every launch has been overwhelming and we are touched by the love of our Cool fans. We are constantly innovating and bringing products that the consumers want. Under the dual-brand strategy we aim to earn a spot within the top 5 smartphone manufacturers in China and in India. Coolpad has a very strong research and development legacy and owns thousands of patents, including one for dual-SIM cards."
Coolpad India, CEO, Mr.Syed Tajuddin also addressed the media and said, "Last couple of years have been a dream run for us in India. We have achieved more than we had planned for and this has only been possible due to our philosophy to bring high quality products with specifications and pricing that consumers want. 2017 will be a game changer for Coolpad in India as we plan to capture 4% of market share. We already have offline presence in 5 cities with a reach in 3000 stores. It is only because of the quality of our products that today Coolpad has a 25% market share on Amazon in the sub Rs.8000 category. Coolpad has now expanded its market to other regions and the smartphones will now be available in Bangladesh and Nepal."
Newly Launched Smartphone Cool 1 has a 5.5 inches 1080p Full High Definition IPS display with 401PPI pixels resolution. It provides a wide viewing experience and better image quality. It has a strong build quality with metallic body that gives Cool 1 a superior and premium look. The handset has 77% Screen-to-body ratio.
Cool 1 is a Dual Sim 4G Smartphone handset. This Smartphone is powered with 1.8 GHz Octacore Snapdragon 652 64Bit Processor MSM8976 Chipset in it that has a support of Adreno 510 GPU for Graphical processing, that helps Cool 1 to run multiple applications effortlessly at the same time means easy and smooth multitasking is available in the Smartphone handset.
The Cool 1 Smartphone handset comes out of the box with Androids Operating System Marshmallow v.6.0.1 with EUI version 5.8.017(Stable) LeEco provided User Interface layer on it.
In the RAM and ROM section, the Cool 1 Smartphone comes with two different variants 3GB and 4GB with the same internal storage of 32GB in the both out of which only 22.90GB of free space is available of the user.
This Smartphone equips with a 13 Megapixels Dual Primary Dual 6P Lens Camera with PDAF technology on its back which has Dual-tone Dual LED flash support. According to the Coolpad its Camera is capable to capture the best even in low light condition. It also supports, a 8- megapixel secondary front camera. and an 8 Megapixel Front Selfie Shooter is available on the front side of the handset. But its camera doesn't has a support for Image stabilization as this feature is not given in the Smartphone by the Coolpad.
On its back panel, right below the Primary Camera, Coolpad has given a Cool Mirror Fingerprint Scanner which works and sense very fast according to the Coolpad. The company says that it has a 360 degrees rotational sensing capability to read and sense easily with some additional features like 0.5s Lock, Fingerprint Payment option, 360⁰ Rotation and Touch To Capture.
Other sensors included in the Smartphone are Accelerometer Sensor, Ambient Light Sensor, Compass/ Megnetometer Sensor, Gyroscope (Infrared controller) Sensor and Proximity Sensor.
This Smartphone packs a 4000mAh lithium ion polymer battery in it with latest Type C Port with Quick charging feature in it. Coolpad also claims its massive 4000mAh battery with fast charging support enables the Smartphone to last upto 450hrs standby time, 150hrs. of Music Playback and a 40hrs. of Movie Playback time. It is a Non-removable battery.
Coming to the Connectivity Options, Cool1 offers Bluetooth 4.1, Wi-Fi 8802.1111 ac/a/b/g/n (2.4/5 GHz), 4G with latest VoLTE feature with GSM : 900/ 1800/ 1900Mhz, WCDMA: 900/ 2100Mhs FDD LTE Band 3/ Band 5 & TDD LTE Band 40 support in India, 2G/3G, GPS, FM Radio, 3.5mm Audio Jack and USB Type-C port. The official said that It also support the Reliance Jio 4G Sim Network.
Dimensions of the Smartphone handset : 152mm X 74.8mm X 8.2mm and it weighs 173gms.
Colors availability : This handset has two color variants Silver and Gold.
Price of the handset : 13,999/- (all inclusive)
Availability of the Smartphone : It will be exclusively available on www.amazon.in in an open sale start from 05th January, 2017.
About Coolpad
Coolpad Group Limited was founded in 1993, and was listed on the Main Board of the Stock Exchange of Hong Kong in December 2004. The Group is a leading developer and provider of integrated solutions for smartphones, mobile data platform systems and value-added business operations in China. The Group provides its products and services to enterprises, government organizations and mobile operators as well as individual consumers in China.
For more information, please refer to : www.coolpadindia.com
What you need to know about COOLPAD:
· 34 million smartphones produced each year
· $3 billion in revenue each year
· First to Introduce most affordable fingerprint enabled smartphone in India
· Launched the world's first CDMA/GSM dual-mode, dual-standby smartphone
· Consumer oriented R&D is our top priority
By GADGETS AND INNOVATIONS - December 29, 2016


It's nice to meet you via A Holy Experience and her Multitudes On Mondays! I'm fairly new to linking up to her site but I love reading the other blogs. I enjoyed your list and will return often!
Nice to meet you, Carrie! Thanks for visiting and I hope you will come back again soon!


I have a 3-yr old Gardenia in the ground. When is the best time of year to transplant it? It a very healthy looking plant in full sun. The new area would be in a indirect sunny location.
Fall is a good time to transplant.


How to Transfer Images to Fabric - Two Awesome ideas!
This UV activated process is non chemical and will wash well (which was always a problem with other ways of transferring images) You can now add your artwork to clothing, tote bags and more. Or just create a large canvas to display artwork as well, but since we are talking wearable art I thought this video and product would be an awesome idea!!
Technology has come a long way, and this is the perfect way to do your own printing in house. Take your artwork or designs you have on your computer and follow the instructions on turning them into a negative and print with the supplies included then watch your creation become real on clothing, bags, whatever is made from natural fibers. It is non toxic and fun to use. An alternative to screen printing.
If you love the idea of wearable or useable art for your crafts to make and sell then here is a great tutorial on just how to print photos onto fabric for all your crafts. There are a few ways to do this, but using the right products really helps.
You can purchase special fabrics for using in your printer, but it can get expensive. You can also make your own fabric paper. It is easy with the right products.
Check out the video below and you can see just how simple and affordable it is to make your own printer fabric especially if you have a lot of crafts to do or you want to have control over the type of fabric being used. You can experiment a bit too.
You can get the supplies needed to do this at craft stores but you can also get the BUBBLEJET SET on Amazon, as well as the FREEZER PAPER SHEETS for doing your transfers.
If you love photography, this is another great way to sell your pictures. Instead of simply framing them (that works too, but many people like wearable and useable art) try adding them to plain tote bags, or purses or add your photos instead of painting a t-shirt free hand.
If you look online you can get good deals on wholesale products such as the white t-shirts, totes or you can create your own "fat quarters" with your very own special photo printed right on the fabric that can be sold for quilting. If you use the bubblejet formula your ink will look as good as a photograph on the fabric.
You may even begin to see your photographs around town as you sell more and more of your work in a useable form. So, instead of framed prints, get creative and find other ways to get your photographs out there for people to enjoy. Happy crafting!
Wearable Art is HOT and Tie Dye Scarf for more useable crafts to make and sell.


Life is too short to be in pain or not be able to live the active life you want. Dr. Mitchell and Dr. Caitlyn both provide chiropractic care for the whole family. Children and adults of all ages and abilities can benefit from professional chiropractic care.


Thank you all for joining us at our August event!
Demian Borba, Product Manager at Adobe, came by to give us some insight into the design thinking process that Adobe used when creating their new prototyping tool: Adobe XD.
This process has allowed them to understand their users' explicit and implicit needs, and turn those into verified features on an agile timeline.
In addition to talking about their design process, Demian also gave a demo of this new product. Some highlights included excellent integration with Illustrator and Sketch, the use of repeaters to quickly apply and update styles to multiple elements, natural interaction management, local layers, and live previewing of designs on mobile devices.
XD is currently available as a part of the Creative Cloud Suite. Check it out!


Gone are the days when it could have been assumed that one can drive without having air condition in his automobile. In order to perform the operation in a predefined way auto air conditioning part are very useful. Internet facility has made it possible to purchase the auto air conditioning parts and even complete systems online and if one manages to carry this part to the car mechanic also, one can reduce the extra charges that had to be paid to the middle man. Now a day's many auto air conditioning parts store have made their websites and costumers can search for the desired part online.
Contacting these online service providers is proved to be very cheap as it reduces the number of people that are involved in the delivery of the auto air conditioning parts. A proportional brake controller senses when and how your tow vehicle brakes by means of an accelerometer or an internal inertia-based sensor. A time-delayed brake controller applies your trailer's brakes when the brake pedal in your tow vehicle is engaged.
A time-delayed brake controller doesn't rely on sensing the braking motions of the tow vehicle. Most time-delayed brake controllers can allow the brakes to pulse on and off when the hazard flashers are activated and the brake controller is set aggressively.
Braking performance: Time-delayed controls always send the same braking power to the trailer brakes. Otherwise, you will need to run a wire from the brake connector location, underneath the vehicle, through the firewall, then to the desired brake control mounting location.
Ground (usually white): This wire is connected from the brake controller to any reliable ground location. Brake switch (usually red): This wire can be found at the switch at the top of the brake pedal.
If a custom wiring adapter is not available for your vehicle, then you'll have to install the brake controller with a universal wiring harness. Note: Vehicle manufacturers change wiring configurations regularly, so use a circuit tester to verify all connections. Via email, text message, or notification as you wait on our site.Ask follow up questions if you need to. At the starter solenoid there is a small wire this is the trigger wire (gives power to solenoid solenoid contacts close and power is given to starter).
I would (and have) recommend your site to others I was quite satisfied with the quality of the information received, the professional with whom I interacted, and the quick response time. Ok-First- According to Hyundai's Normal Service Schedule, the Timing Belt should be replaced at intervals of every 60,000 miles or 48 months, whichever comes first.
What about me paying for a timing belt change after only 11,000 of driving; does it or should it be done? Yesterday's race at the Indianapolis Motor Speedway proved to be another letdown for the paltry few NASCAR fans in attendance.
There was reason for excitement going into the event, which promised to be Tony Stewart's final Brickyard 400 and Jeff Gordon's (uh, second consecutive) final Brickyard 400. But while most of those in attendance were left disappointed by the cars speeding single-file around the track, they did at least get to enjoy the ones parked over at the Chevy Display. At last year's Brickyard, Chevrolet brought along the then-new, sixth-generation 2016 Camaro. It certainly looks the part, with a Nightfall Grey paint job highlighted by orange and satin chrome accents, along with unique "FIFTY" badging throughout the exterior and interior.
Chevy has yet to put a price on the 50th Anniversary Camaro, but the automaker says that the commemorative vehicle will be going on sale sometime this summer. If you missed its appearance at the Brickyard, you can see the anniversary Camaro when it makes another public appearance at next month's Woodward Dream Cruise in Detroit. At Woodward, the 2017 Chevy Camaro 50th Anniversary Edition should be the highlight of a relatively straightforward parade—just like it was at yesterday's Brickyard 400.
Patrick GrieveEditorPatrick Grieve was born in Southwestern Ohio and has lived there all of his life, with the exception of a few years spent getting a Creative Writing degree in Southeastern Ohio. The trend of using air condition is not only changed in those parts of the world which remain hot throughout the year, but in those as well where climate is hot only for a few months.
Replacements are offered for the parts like accumulators, condensers, compressors, expansion dries, repair manuals and retro items compatible to the EPA regulations.
These parts can be searched through different criteria like by car make, model of the car and year of manufacture. These manufacturers also claim to have enough expertise and knowledge that they can help the costumers to select the right parts either by e mail or by telephone. In this way one can directly order the parts from their manufacturers and thus the share of middle man is eliminated and the parts become very cheap for the costumer. Some vehicles with factory tow packages already have a wire for this connection, and it would run from under the dash to the rear of the vehicle.
Remove the wire and with the key in the start position check for battery voltage on this small wire.
There is no power to the starter and check under the dash with a light tester and have power everywhere. In the end, though, neither of the two fan favorite Indiana drivers could do much to compete with Kurt Busch, who led 149 of 170 laps en route to a particularly uninspiring victory. For many, including myself, it was the first time we got up close and personal with the new 2017 Chevy Camaro 50th Anniversary Edition.
He loves to take road trips, sometimes to places as distant as Northeastern or even Northwestern Ohio. The effect of change in the behavior of drivers can be easily observed in the areas where driving a car without air conditioning is still not so common. 2 wires to starter from battery and wire from soleniod on starter and goes into the firewall.
Patrick also enjoys old movies, shopping at thrift stores, going to ballgames, writing about those things, and watching Law & Order reruns. Additionally there are many forums where the costumers can ask their quires which are answered by the professionals. However, if you are not experiencing any drivability issues such as skipping, stalling, shifting problems, etc, then more than likely, the problem is going to lie in the EVAP (emissions) system. If this belt is to break while under load, like at highway speeds, it can cause major damage to other internal engine parts, resulting in a much higher repair bill. So some how that switch is in between the power and the starter, that is why it is a safety switch. Very commonly, this could be a faulty, loose or missing fuel cap or even putting fuel in while the vehicle is running. Once you get these p-codes you can more accurately and efficiently diagnose the problem and then make the repair.
If you still need help after retrieving the codes, you can bring them here, and any of our Techs can walk you through the diagnostic procedure, and then even help you make the repair if you feel confident enough to try.
09.01.2015 at 20:33:35 Annually, according to a examine released in the present day flood harm, or odometer.
09.01.2015 at 14:48:10 Inside combustion engines in the identical class save doesn't cease with quotes are free to generate.
09.01.2015 at 22:10:10 Endorsement is subject to the terms and situations contained tie.


JAKARTA - Dozens of tourists were evacuated on Sunday from a waterfall site hit by landslides in the Indonesian holiday island of Lombok, where two people died, authorities said.
Two moderate earthquakes struck Lombok on Sunday afternoon, triggering the landslides when tourists were visiting the Tiu Kelep waterfall, in north of the island, Sutopo Purwo Nugroho, a spokesman for the disaster agency, said in a statement.The disaster agency said the two people who died were both Malaysians, but state news agency Antara reported only one was a Malaysian national.
Fourteen Indonesians and 22 other Malaysians were rescued from the waterfall site, Nugroho said.


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October, Issue 3
Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder
Giuseppe Lippi [*] [1] Paola Avanzini [1] Rosalia Aloe [1] Gianfranco Cervellin [2]
Show more about author
[1] Clinical Chemistry Laboratory, Academic Hospital of Parma, Parma, Italy
[2] Emergency Department, Academic Hospital of Parma, Parma, Italy
[*] Corresponding author: ,
Introduction: Blood collection through intravenous lines frequently causes spurious hemolysis. Due to specific structure, the tube holder Holdex® (Greiner Bio-One GmbH, Kremsmuenster, Austria) is supposed to prevent erythrocyte injury in samples collected from catheters, so that we planned a specific study to support this hypothesis.
Materials and methods: Blood was collected from emergency department (ED) patients with 20-gauge catheter. In patients with odd order numbers, first and second tubes were collected with conventional holder (BD Vacutainer One Use Holder, Becton Dickinson, Milan, Italy) and the third with Holdex, whereas in even patients first and second tubes were drawn with Holdex and the third using BD Vacutainer One Use Holder. The first tube was discarded, whereas the second and third were centrifuged and serum was tested for potassium, lactate dehydrogenase (LD) and hemolysis index.
Results: The final study population consisted in 60 ED patients. Concentrations of potassium (4.25 vs. 4.16 mmol/L; P = 0.031), LD (498 vs. 459 U/L; P = 0.039) and cell-free hemoglobin (0.42 vs. 0.22 g/L; P = 0.042) were higher in samples collected with BD Vacutainer One Use Holder than with Holdex. The mean bias of cell-free hemoglobin was -0.4 g/L in samples collected with Holdex. Although the frequency of samples with cell-free hemoglobin > 0.5 g/L was identical (17/60 vs. 17/60; P = 1.00), the frequency of those with concentrations >3.0 g/L was higher using BD Vacutainer One Use Holder than Holdex (4/60 vs. 0/60; P = 0.042).
Conclusions: The use of Holdex for drawing blood from intravenous lines may be effective for reducing gross hemolysis.
The manually intensive activities of venous blood collection represent a leading source of variability throughout the total testing process (1). Spurious hemolysis, in particular, is the main cause of unsuitable samples in most clinical laboratories, where the frequency of hemolyzed serum or plasma can be as high as 3% of all samples referred for routine and stat testing, and may even reach 8% of all samples collected in the emergency department (ED) (2). Spurious hemolysis, also known as "in vitro" hemolysis, is commonly referred to as breakdown of erythrocytes during blood collection, handling, transportation and storage (3). In routine laboratory practice, spurious hemolysis is typically classified according to concentration of cell-free hemoglobin that may be present in serum or plasma, so that it can be considered "mild" for hemoglobin concentration comprised between 0.5-1.0 g/L (pink to slightly red hue), "frank" for hemoglobin concentration between 1.0-3.0 g/L (red hue), and "gross" for hemoglobin concentration > 3.0 g/L (red to brown hue), respectively (4). In the presence of gross hemolysis, all test results are virtually affected, no corrective formulas seem effective to overcome the bias, and samples should hence be rejected (5).
Among the various mechanisms that may cause spurious erythrocyte injury, collection of venous blood through intravenous lines (i.e., catheters) is indeed prevailing in short stay units such as EDs or intensive care units (ICUs), where this practice represents a faster, easier and more convenient alternative than searching for another venipuncture site (6). The postulated mechanisms supporting a higher chance of hemolysis when blood is drawn from catheters include the presence of valves inside the collection system, the collapse of tube for high pressure aspiration, the placing of catheters in unusual sites (e.g., smaller distal veins) and use of large needles (e.g., greater than 20 gauge). Another potential mechanism is attributable to the higher shear stress caused by difference of pressure between veins and evacuated tube systems (6). Some potential solutions have already been proposed to overcome this last aspect, including the use of low volume vacuum tubes (7,8) or the manual aspiration of blood using syringe or closed collection systems such as S-Monovette® (Sarstedt AG & Co., Nümbrecht, Germany) (9,10), which may globally reduce the burden of spurious hemolysis.
Another potential solution is represented by the use of specific collection systems that may be capable to attenuate the difference of pressure between vein and vacuum tubes. The Holdex® (Greiner Bio-One GmbH, Kremsmuenster, Austria) is a sterile, transparent disposable tube holder made from polypropylene, which is characterized by an eccentric luer on the top and a stainless steel needle inside the holder (Figure 1). Due to this specific structure, the blood flow through the system encounters two angles of 90°, which - according to the manufacturer's declaration - would attenuate the delta of pressure existing between vein and vacuum tube when collecting blood from catheters, ultimately preventing erythrocyte injury, so that we planned a specific study to support this hypothesis.
Figure 1. Structure of (a) conventional evacuated tube holder (i.e., BD Vacutainer One Use Holder) and (b) Holdex.
This randomized prospective investigation was settled in a large urban ED, averaging ~90,000 visits per year. The study population consisted in 60 consecutive patients admitted from 8:00 AM to 2:00 PM of a single working day, who required blood collection for diagnostics purposes, including assessment of potassium and lactate dehydrogenase (LD). Each patient provided an informed consent for being enrolled in this study, which was in accord with the ethical standards established by the institution in which the experiments were performed and the Helsinki Declaration of 1975.
According to our experimental design, blood was drawn by the same two nurses in duty at the ED through a 1.0 x 3.2 mm, 20-gauge catheter (Neo DELTA VEN, Viadana, MN, Italy; ref. n. 1331), placed in a vein of the upper limb.
In patients with odd order numbers (i.e., patients 1, 3, 5, etc), three consecutive 13 x 100 mm, 5.0 mL Vacutainer® SST II Plus serum tubes (Becton Dickinson Italia S.p.A., Milan, Italy) were collected. The first and the second tubes were drawn using a conventional Becton Dickinson (BD) tube holder (BD Vacutainer One Use Holder, Becton Dickinson), whereas the third tube was collected using Holdex® (Greiner Bio-One GmbH, Kremsmuenster, Austria). In the even group of patients (i.e., patients 2, 4, 6, etc.), the first and the second tubes were collected using Holdex, whereas the third tube was collected using BD Vacutainer One Use Holder. In the patients, the first serum tube was filled immediately after catheter insertion and then discarded, whereas the following tubes were transported to the core laboratory within 20 to 40 min after collection, where they were subjected to standard centrifugation according to manufacturers' instructions (1300 x g for 10 min at room temperature). All blood tubes were collected and filled up to the nominal volume, and all phases of sample collection were accurately standardized.
Serum was tested for potassium (indirect ISE), LD (DGKC method) and hemolysis index (i.e., HI) on Beckman Coulter DxC, using proprietary reagents (Beckman Coulter Inc., Brea CA, USA). In this instrument, the HI is assessed by direct spectrophotometry, and a previous study has proven that this measure provides results of cell-free hemoglobin that are comparable with those of the reference cyanmethemoglobin assay (11). All tests were performed in duplicate, and results were finally averaged.
Normality distribution was verified by Kolmogorov-Smirnov test and concentrations were presented as mean and 95% confidence intervals (95% CI). The statistical analysis included Wilcoxon test for paired samples (for continuous variables), chi-squared test (for categorical variables) and analysis of bias by Bland & Altman plots. The statistical evaluation was performed with Analyse-it for Microsoft Excel version 2.24 (Analyse-it Software Ltd, Leeds, UK). Statistical significance was set at P < 0.05.
The main results of this study are synthesized in table 1. The final study population consisted in 60 ED patients (32 females and 28 males; median age 52 years, range 37-74 years). The sites of catheter insertion were the median cubital or basilic veins (22/60; 0.37), the median antebrachial vein (15/60; 0.25), cephalic vein (12/60; 0.20), basilic vein (5/60; 0.08) and veins of the metacarpal plexus (6/60; 0.10). In four patients the insertion was unsuccessful in the median cubital vein, and needed to be repeated on the opposite arm. Activity of LD and the concentrations of potassium and cell-free hemoglobin were found to be significantly higher in samples collected with the conventional BD Vacutainer One Use Holder than using Holdex (Table 1). The mean bias of cell-free hemoglobin in samples collected with Holdex was -0.4 g/L (95% CI, -0.9 to 0.1 g/L) as compared with BD Vacutainer One Use Holder. Although no statistically significant difference could be observed in the frequency of samples with concentration of cell-free hemoglobin > 0.5 g/L (i.e., 17/60 vs. 17/60; Pearson's X2 statistic: 0.00; P = 1.000), the frequency of samples with gross hemolysis (i.e., > 3.0 g/L, found in 2 samples collected from veins of metacarpal plexus, 1 from median basilic and 1 from cephalic veins) was significantly higher in samples collected with BD Vacutainer One Use Holder than with Holdex (i.e., 4/60 vs. 0/60; Pearson's X2 statistic: 4.14; P = 0.042) (Figure 2).
Table 1. Concentration of potassium and cell-free haemoglobin and lactate dehydrogenase (LD) activity in serum samples drawn from intravenous line using a conventional tube holder (BD Vacutainer One Use Holder) and Holdex.
Figure 2. Distribution of cell-free hemoglobin concentration in serum samples drawn from intravenous line using a conventional tube holder (BD Vacutainer One Use Holder) and Holdex. The horizontal dotted line defines the threshold of gross hemolysis.
Spurious hemolysis is a major issue in laboratory diagnostics, due to its relatively high frequency and the economical, organizational and clinical issues that may be associated with receipt of hemolyzed blood for routine or urgent testing (3,4). The presence of a large amount of cell-free hemoglobin in serum or plasma (i.e., exceeding 3.0 g/L) may in fact cause suppression of virtually all tests, since the bias may cause an unavoidable degree of biological and/or analytical interference (5). The identification of potential solutions that may be effective to overcome this frequent problem is hence advisable.
It has been clearly established that blood collection through intravenous lines is an important cause of erythrocyte injury, which has been at least partially attributed to remarkable difference of pressure existing between vein and vacuum tubes (6). The development of tube holders specifically suited for collecting blood from intravenous lines would hence represent a breakthrough for decreasing the risk of obtaining unsuitable samples and consequently lowering healthcare costs and patient discomfort. The difference between the Holdex tube holder and other standard devices is represented by the eccentric luer needle adapter, which allows a flat venipuncture angle (Figure 1). One advantage of this system is represented by the fact that the blood can be seen clearly flowing through the translucent adapter when venipuncture has been successfully carried out. Another claimed benefit is the lower risk of producing spurious hemolysis when collecting blood from catheters or butterfly needles. In fact, while in a standard holder the blood follows a direct linear flow from the venipuncture site into blood collection tubes, the blood flow in Holdex is interrupted by the presence of two angles of 90°, which finally attenuate the gap of pressure existing between veins and vacuum tubes.
In a previous study, Almagor and Lavid-Levy assessed within-subject variations in the results of some blood analyses in blood collected by straight needles using Greiner or the BD systems, and found that plasma hemoglobin was higher using the former system (12). Although the authors theorized that the variation in linear flow of blood may generate a mechanical strain on blood cells, thus affecting membrane integrity and causing efflux of intracellular constituents into the surrounding serum, the difference was globally modest (i.e., 0.25 vs. 0.16 g/L; P < 0.025) and still lower than 0.5 g/L, that is the conventional threshold of "mild" hemolysis. In a following cross-sectional study, Romero Ruiz et al. compared blood collection by standard venipuncture using Holdex, BD system and syringe, and showed that the rate of hemolysis did not differ among the three collection systems (i.e., 16% with Holdex, 15% with BD and 23% with syringe) (13). In both studies, however, no data were provided about the use of Holdex for collecting blood from intravenous lines.
The results of this study, which is the first that has assessed the impact of Holdex on collecting blood from catheters, show that although the typical structure of the system may be a (relative) drawback when collecting blood with straight needles (12), it may turn to be a tangible advantage when blood is drawn from intravenous lines. It is noteworthy, however, that the distribution of concentrations was largely overlapping and that the P values were also modest, so that the global effect must be weighed against these data. Although the overall rate of visually hemolyzed specimens (i.e., cell-free hemoglobin > 0.5 g/L) was identical (i.e., 0.28), the frequency of those with gross hemolysis (i.e., cell-free hemoglobin > 3.0 g/L) was substantially higher using a conventional tube holder than with Holdex. It is also noteworthy that the concentration of cell-free hemoglobin, as well as that of two indices of hemolysis such as potassium and LD activity, was also higher in samples drawn with a conventional tube holder, thus implicitly confirming that the erythrocyte injury may be attenuated with this novel device. We thereby conclude that use of Holdex for collecting blood from intravenous lines may be mildly effective to reduce the risk of obtaining grossly hemolyzed specimens.
The authors acknowledge the nurses Maria Pia Carpi, Caterina Colombo and Davide Caputo for the skill assistance during blood collection in the emergency department.
Potential conflict of interest
None declared
1. Simundic AM, Lippi G. Preanalytical phase--a continuous challenge for laboratory professionals. Biochem Med 2012;22:145-9. http://dx.doi.org/10.11613/BM.2012.017.
2. Lippi G, Salvagno GL, Favaloro EJ, Guidi GC. Survey on the prevalence of hemolytic specimens in an academic hospital according to collection facility: opportunities for quality improvement. Clin Chem Lab Med 2009;47:616-8. http://dx.doi.org/10.1515/CCLM.2009.132.
3. Simundic AM, Topic E, Nikolac N, Lippi G. Hemolysis detection and management of hemolysed specimens. Biochem Med 2010;20:154-9. http://dx.doi.org/10.11613/BM.2010.018.
4. Lippi G, Plebani M, Di Somma S, Cervellin G. Hemolyzed specimens: a major challenge for emergency departments and clinical laboratories. Crit Rev Clin Lab Sci 2011;48:143-53. http://dx.doi.org/10.3109/10408363.2011.600228.
5. Lippi G, Avanzini P, Pavesi F, Bardi M, Ippolito L, Aloe R, Favaloro EJ. Studies on in vitro hemolysis and utility of corrective formulas for reporting results on hemolyzed specimens. Biochem Med 2011;21:297-305. http://dx.doi.org/10.11613/BM.2011.040.
6. Lippi G, Cervellin G, Mattiuzzi C. Critical review and meta-analysis of spurious hemolysis in blood samples collected from intravenous catheters. Biochem Med 2013;23:193-200. http://dx.doi.org/10.11613/BM.2013.022.
7. Cox SR, Dages JH, Jarjoura D, Hazelett S. Blood samples drawn from IV catheters have less hemolysis when 5-mL (vs 10-mL) collection tubes are used. J Emerg Nurs 2004;30:529-33. http://dx.doi.org/10.1016/j.jen.2004.10.004.
8. Petit N, Ziegler L, Goux A. Reduction of haemolysis rates using intravenous catheters with reduced capacity tubes. Ann Biol Clin (Paris);60:471-3.
9. Lippi G, Avanzini P, Musa R, Sandei F, Aloe R, Cervellin G. Evaluation of sample hemolysis in blood collected by S-Monovette using vacuum or aspiration mode. Biochem Med 2013;23:64-9. http://dx.doi.org/10.11613/BM.2013.008.
10. Lippi G, Avanzini P, Cervellin G. Prevention of hemolysis in blood samples collected from intravenous catheters. Clin Biochem 2013;46:561-4. http://dx.doi.org/10.1016/j.clinbiochem.2013.01.021.
11. Lippi G, Luca Salvagno G, Blanckaert N, Giavarina D, Green S, Kitchen S, et al. Multicenter evaluation of the hemolysis index in automated clinical chemistry systems. Clin Chem Lab Med 2009;47:934-9. http://dx.doi.org/10.1515/CCLM.2009.218.
12. Almagor M, Lavid-Levy O. Effects of blood-collection systems and tubes on hematologic, chemical, and coagulation tests and on plasma hemoglobin. Clin Chem 2001;47:794-5.
13. Romero Ruiz A, Tronchoni de los Llanos J, Sánchez Negrete J. Hemolysis in blood samples. Assessment in 3 extraction systems. Rev Enferm 2004;27:19-22.
License: CC-BY-NC-ND
Date received: 15.05.2013
Date accepted: 16.07.2013
Pages: 303 - 307
DOI: 10.11613/BM.2013.036
Categories: Original papers
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Lippi G, Avanzini P, Aloe R, Cervellin G. Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder. Biochem Med (Zagreb). 2013;23:303-307


After graduating from Mizzou with a Bachelors in Economics and an emphasis in mathematics, Blake began his career at Lind-Waldock as an order specialist and quickly rose to the ranks of Desk Supervisor. He was then promoted to the Lind-Plus division where he spent 15 years assisting clients. His methodology is to read as much fundamental research that he can get his hands on, and then employ proprietary technical indicators to time buy-sell decisions. He uses ADMIS fundamental research along with proprietary technical indicators and strict money management principles to help clientele navigate these markets.
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International Journal of Systematic and Evolutionary Microbiology — Recommend this title to your library
International Journal of Systematic and Evolutionary Microbiology - Volume 67, Issue 11, 2017
Proteobacteria
Quisquiliibacterium transsilvanicum gen. nov., sp. nov., a novel betaproteobacterium isolated from a waste-treating bioreactor
Tamás Felföldi, Róbert Dezső Fikó, Anikó Mentes, Erika Kovács, István Máthé, Peter Schumann and Erika Tóth
https://doi.org/10.1099/ijsem.0.002368
A new betaproteobacterium, CGI-09T, was isolated from an activated sludge bioreactor which treated landfill leachate. Based on 16S rRNA gene sequence analysis, the new strain shared the highest pairwise similarity values with members of the order Burkholderiales : Derxia gummosa IAM 13946T (family Alcaligenaceae ), 93.7 % and Lautropia mirabilis DSM 11362T (family Burkholderiaceae ), 93.6 %. Cells of strain CGI-09T were rod-shaped and non-motile. The new strain was oxidase and catalase positive and capable of reducing nitrate to nitrite. The predominant fatty acids were C16 : 1 ω7c, C16 : 0, cycloC17 : 0 and C18 : 1 ω7c, the major respiratory quinone was Q-8, and the detected polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA of strain CGI-09T was 70.2 mol%. The new bacterium can be distinguished from the members of genera Derxia and Lautropia based on its non-motile cells, arginine dihydrolase activity, its high cyclo C17 : 0 fatty acid content and the lack of hydroxy fatty acids. On the basis of the phenotypic, chemotaxonomic and molecular data, strain CGI-09T is considered to represent a new genus and species within the family Burkholderiaceae , for which the name Quisquiliibacterium transsilvanicum gen. nov., sp. nov. is proposed. The type strain is CGI-09T (=DSM 29781T=JCM 31785T).
Salsuginimonas clara gen. nov., sp. nov., a member of the family Pseudoalteromonadaceae isolated from a brackish river
Shih-Yi Sheu, Pei-Bei Xie, Ceshing Sheu and Wen-Ming Chen
A bacterial strain designated LSN-49T was isolated from a brackish river in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LSN-49T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate accumulating, motile by means of a monopolar flagellum, non-spore forming, straight rods and formed shiny and translucent colonies. Growth occurred at 20–40 °C (optimum, 25–30 °C), at pH 6–10 (optimum, pH 7–8) and with 0–3 % (w/v) NaCl [optimum, 0–1 % (w/v)]. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω8c and C16 : 0. The polar lipid profile consisted of a mixture of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine, (PC), two uncharacterized aminophospholipids (APL1 and APL2), one uncharacterized glycolipid (GL1), four uncharacterized phospholipids (PL1–PL4) and four uncharacterized lipids (L1–L4). The major polyamine was putrescine. The major isoprenoid quinone was Q-8 and the DNA G+C content was 51.0 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that LSN-49T formed a distinct lineage with respect to closely related genera in the family Pseudoalteromonadaceae . LSN-49T was most closely related to Pseudoalteromonas , Algicola and Psychrosphaera and showed 89.3–92.1 % sequence similarity with members of the family Pseudoalteromonadaceae with validly published names. On the basis of the genotypic and phenotypic data, LSN-49T represents a novel genus and species of the family Pseudoalteromonadaceae , for which the name Salsuginimonas clara gen. nov., sp. nov. is proposed. The type strain is LSN-49T (=BCRC 81005T=LMG 29726T=KCTC 52439T).
Paraglaciecola aestuariivivens sp. nov., isolated from a tidal flat
Sooyeon Park, Su Jung Choi, Jeehyun Choi and Jung-Hoon Yoon
A Gram-stain-negative, singly flagellated, aerobic and coccoid, ovoid or rod-shaped bacterium, designated strain JDTF-33T, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-33T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, demonstrated that strain JDTF-33T belonged to the genus Paraglaciecola , joining the type strain of the species Paraglaciecola aquimarina with 97.9 % sequence similarity. Strain JDTF-33T exhibited 16S rRNA gene sequence similarities of 97.1 and 97.0 % to the type strains of Paraglaciecola arctica and Paraglaciecola psychrophila , respectively, and of 96.1–96.6 % to the type strains of the other species of the genus Paraglaciecola . Strain JDTF-33T showed DNA–DNA relatedness values of 11–24 % to the type strains of the species P. aquimarina , P. arctica and Paraglaciecola psychrophila . Strain JDTF-33T contained Q-8 as the predominant ubiquinone and summed feature 3 (C16 : ω7c and/or C16 : 1ω6c) and C16 : 0 as the major fatty acids. The major polar lipids detected in strain JDTF-33T were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain JDTF-33T was 41.7 mol%. Differential phenotypic properties, together with phylogenetic and genetic data, demonstrate that strain JDTF-33T is separate from species of the genus Paraglaciecola with validly published names . On the basis of the data presented, strain JDTF-33T represents a novel species of the genus Paraglaciecola , for which the name Paraglaciecola aestuariivivens sp. nov. is proposed. The type strain is JDTF-33T (=KCTC 52838T=NBRC 112782T).
Paracoccus litorisediminis sp. nov., isolated from a tidal flat
Sooyeon Park, Jeehyun Choi, Su Jung Choi and Jung-Hoon Yoon
A Gram-stain-negative, non-spore-forming, non-motile and coccoid, ovoid or short rod-shaped bacterial strain, designated GHD-05T, was isolated from a tidal flat on the Yellow Sea in South Korea. Strain GHD-05T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain GHD-05T belonged to the genus P aracoccus , clustering with the type strain of P aracoccus aestuariivivens. Strain GHD-05T exhibited 16S rRNA gene sequence similarity values of 97.0–99.1 % to the type strains of P aracoccus aestuariivivens, P aracoccus limosus, P aracoccus laeviglucosivorans and P aracoccus marinus, and of 94.2–96.9 % to the type strains of the other Paracoccus species. Strain GHD-05T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids detected in strain GHD-05T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified glycolipid. The DNA G+C content of strain GHD-05T was 64.1 mol% and its DNA–DNA relatedness values with the type strains of P. aestuariivivens , P aracoccus limosus, P. laeviglucosivorans and P. marinus were 13–32 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain GHD-05T is separated from recognized Paracoccus species. On the basis of the data presented here, strain GHD-05T is considered to represent a novel species of the genus Paracoccus , for which the name Paracoccus litorisediminis sp. nov. is proposed. The type strain is GHD-05T (=KCTC 52978T=NBRC 112902T).
Acidocella aquatica sp. nov., a novel acidophilic heterotrophic bacterium isolated from a freshwater lake
Rei Okamoto, Hisaya Kojima and Manabu Fukui
A novel acidophilic heterotrophic bacterium, strain Ok2GT, was isolated from a freshwater lake in Japan. Cells of the isolate were Gram-stain-negative and non-motile rods (0.6–0.8×1.0–2.8 µm). Growth was observed at 4–35 °C with an optimum growth temperature of 28 °C. The pH range for growth was 3.0–6.2 with an optimum pH of 4.5. The strain utilized fructose, glucose, sucrose, mannitol, sorbitol, ethanol, benzyl alcohol, pyruvate, yeast extract and tryptone as carbon and energy sources for aerobic growth. DNA G+C content was 62.6 mol%. The major cellular fatty acid and the isoprenoid quinone were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and Q-10, respectively. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain Ok2GT belongs to the genus Acidocella but is distinct from existing species with sequence similarities lower than 97 %. On the basis of these results, strain Ok2GT (=NBRC 112502T=DSM 104037T) is proposed as the type strain of a novel species, Acidocella aquatica sp. nov.
Characterization of Marinomonas algicida sp. nov., a novel algicidal marine bacterium isolated from seawater
Sylvia Kristyanto, Dhiraj Kumar Chaudhary, Sang-Seob Lee and Jaisoo Kim
A novel Marinomonas -like, aerobic, Gram-reaction-negative, moderately halophilic, acidophilic, motile by a single polar flagellum, non-spore-forming, rod-shaped bacterium that showed algalytic activity, designated strain Yeongu 1-4T, was isolated from surface seawater of Geoje Island in the South Sea, Republic of Korea. The strain was oxidase-negative and weakly positive for catalase. Growth of this bacterium was observed at temperatures from 4 to 42 °C, at salinities from 0 to 12 % and at pH from 4.5 to 9.0, and it was not able to degrade starch, gelatin, casein or Tween 80. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Yeongu 1-4T was related most closely to Marinomonas spartinae SMJ19T with similarity of 99.3 %. However, levels of DNA–DNA relatedness between strain Yeongu 1-4T and the most closely related species were lower than 70 %, confirming that they represent distinct genomic species. The genomic DNA G+C content of strain Yeongu 1-4T was 44.2 mol%. The organism used Q-8 as the predominant respiratory quinone, and C16 : 1ω7c, C18 : 1ω7c and C16 : 0 as major cellular fatty acids. Based on data from this polyphasic taxonomic study, strain Yeongu 1-4T belongs to a novel species of the genus Marinomonas , within the family Oceanospirillaceae , for which the name Marinomonas algicida is proposed. The type strain is Yeongu 1-4T (=KEMB 9005-327T=MCCC 1K00609T).
Planktotalea lamellibrachiae sp. nov., isolated from a marine organism in Kagoshima Bay, Japan
Yuichi Nogi, Shinro Nishi and Sumihiro Koyama
A novel marine bacterial strain, designated JAM 119T, was isolated from a tubeworm trophosome in Kagoshima Bay, Japan. Cells were Gram-negative, rod-shaped, non-spore-forming aerobic chemoorganotrophs and motile by means of a single polar flagellum. The isolate grew optimally at 25–27 °C and in the presence of 3 % NaCl. The major respiratory quinone was Q-10. The predominant fatty acid was C18 : 1ω7c. Phosphatidylcholine, phosphatidylglycerol and an unidentified aminolipid were the major polar lipids. On the basis of 16S rRNA gene sequence analysis, the isolated strain was closely affiliated with members of the genus Planktotalea in the class Alphaproteobacteria , and the 16S rRNA gene sequence similarity of the new isolates with the closest related species, Planktotalea frisia SH6-1T, was 97.3 %. The DNA G+C content of the novel strain was 57.0 mol%. Based on differences in taxonomic characteristics, the isolated strain represents a novel species of the genus Planktotalea , for which the name Planktotalea lamellibrachiae sp. nov. (type strain JAM 119T; JCM 31859T=DSMZ 104669T) is proposed.
Psychrosphaera aquimarina sp. nov., a marine bacterium isolated from seawater collected from Asan Bay, Republic of Korea
Sophea Pheng, Niraikulam Ayyadurai, A-Young Park and Song-Gun Kim
Cells of strain SW33T, isolated from the seawater of Asan Bay, Republic of Korea, were characterized as Gram-stain-negative, aerobic, rod-shaped, motile and non-spore-forming. Phylogenetic analysis revealed that strain SW33T belonged to the genus Psychrosphaera and clustered distantly with the other genera in the family Pseudoalteromonadaceae in the phylogenetic tree. The 16S rRNA sequences of strain SW33T revealed high similarities to Psychrosphaera saromensis SA4-48T (98.7 %), Psychrosphaera haliotis KDW4T (97.4 %) and Psychrosphaera aestuarii PSC101T (97.3 %). The major fatty acids were C16 : 0 (27.9 %), summed feature 3 (32.2 %) and summed feature 8 (17.2 %). The predominant quinone was Q-8, and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified amino lipid. The DNA G+C content was 38.3 mol%. The DNA–DNA relatedness values with the three species of Psychrosphaera saromensis KCTC 23240T, Psychrosphaera haliotis KCTC 22500T and Psychrosphaera aestuarii KCTC 32274T were 22, 23 and 18 %, respectively. Based on the phenotypic characteristics and taxonomic analyses, we propose that strain SW33T represents a novel species within the genus Psychrosphaera , for which the name Psychrosphaera aquimarina sp. nov. with the type strain SW33T (=KCTC 52743T=CICC 24249T) is proposed.
Roseomonas terricola sp. nov., isolated from agricultural soil
Dong-Uk Kim, Hyosun Lee, Song-Gun Kim and Jong-Ok Ka
A novel strain, designated EM302T, was isolated from a soil sample collected from farmland located in Yesan-gun in the Republic of Korea. The cells were Gram-stain-negative, aerobic, non-motile without flagella and coccobacilli-shaped. Colonies were circular with entire edges, convex, opaque and pink. The strain grew at 15–40 ˚C (optimum, 35 ˚C) and pH 6.0–9.0 (optimum, 7.0). The strain grew in R2A medium without the addition of NaCl. Based on 16S rRNA gene sequences analysis, strain EM302T was found to be phylogenetically related to Roseomonas wooponensis WW53T (96.5 % 16S rRNA gene sequence similarity), Roseomonas arcticisoli MC 3624T (95.2 %), Roseomonas aerilata 5420-30T (94.5 %), Roseomonas pecuniae N75T (94.4 %) and Roseomonas vinacea CPCC 100056T (94.4 %). The major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0. The predominant respiratory quinone was Q-10. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminolipid. The DNA G+C content of the type strain was 65.7 mol%. On the basis of data presented here, strain EM302T is considered to represent a novel species of the genus Roseomonas , for which the name Roseomonas terricola sp. nov. is proposed. The type strain is EM302T (=KACC 13942T=KCTC 42906T=NBRC 111477T).
Oceanibaculum nanhaiense sp. nov., isolated from surface seawater
Yaping Du, Xiupian Liu, Qiliang Lai, Weiwei Li, Fengqin Sun and Zongze Shao
A taxonomic study was carried out on strain L54-1-50T, which was isolated from surface seawater of the South China Sea. Cells of strain L54-1-50T were Gram-stain-negative, rod-shaped, oxidase-positive and catalase-positive. Growth was observed at salinities from 0 to 9 % (optimum 2 %, w/v), at pH 6.0–10.0 (optimum 8.0–9.0) and at temperatures from 10 to 45 °C (optimum 25–37 °C), but not at 4 or 50 °C. The 16S rRNA gene sequence analysis indicated that strain L54-1-50T was a member of the genus Oceanibaculum , related to Oceanibaculum indicum P24T (98.8 %) and Oceanibaculum pacificum MC2UP-L3T (97.7 %). The digital DNA–DNA hybridization values between strain L54-1-50T and the two type strains O. indicum P24T and O. pacificum MC2UP-L3T were 35.4±2.5 and 23.7±2.5 %, respectively. The average nucleotide identity values between strain L54-1-50T and two type strains were 79.7 and 88.3 %, respectively. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C16 : 0 and C18 : 1 2-OH. The respiratory quinone was Q-10. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, two unidentified phospholipids and three unidentified lipids. The G+C content of the chromosomal DNA was 65.1 mol%. The combined genotypic and phenotypic data showed that strain L54-1-50T represents a novel species of the genus Oceanibaculum , for which the name Oceanibaculum nanhaiense sp. nov. is proposed, with the type strain L54-1-50T (=KCTC 52312T=MCCC 1A05150T).
Altererythrobacter mangrovi sp. nov., isolated from mangrove sediment
Hu Liao, Yuqian Li, Mengjia Zhang, Xiaolan Lin, Qiliang Lai and Yun Tian
A Gram-stain-negative, facultative anaerobic, yellow-pigmented, rod-shaped, non-flagellum and non-motile bacterial strain, designed C9-11T, was isolated from mangrove sediment in Zhangzhou, Fujian Province, China. Growth was observed at temperatures from 15 to 37 °C (optimum 30 °C), at salinities from 0 to 4 % (optimum 0.5–2 %) and at pH from 6 to 8 (optimum 7). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain C9-11T was a member of the genus Altererythrobacter . Strain C9-11T was related most closely to Altererythrobacter oceanensis Y2T (96.8 % 16S rRNA gene sequence similarity), followed by Altererythrobacter marinus H32T (96.5 %). The G+C content of the genomic DNA was 63.5 mol%. The dominant fatty acids were C18 : 1ω7c (41.6 %), C17 : 1ω6c (15.4 %), 11-methyl C18 : 1ω7c (8.1 %), summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c 7.4 %) and C16 : 0 (6.2 %). The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. According to its morphology, physiology, fatty acid composition and 16S rRNA gene sequence analysis, strain C9-11Tbelongs to genus Altererythrobacte r, but can readily be distinguished from recognized species. Strain C9-11T is proposed as the type strain of a novel species of the genus Altererythrobacte r, for which the name Altererythrobacter mangrovi sp. nov (=MCCC 1K03311T=JCM 32056T) is proposed.
Siccirubricoccus deserti gen. nov., sp. nov., a proteobacterium isolated from a desert sample
Zi-Wen Yang, Nimaichand Salam, Zheng-Shuang Hua, Bing-Bing Liu, Ming-Xian Han, Bao-Zhu Fang, Dong Wang, Min Xiao, Wael N. Hozzein and Wen-Jun Li
Strain SYSU D8009T was isolated from a desert sample collected from Saudi Arabia. The taxonomic position of the isolate was investigated by a polyphasic approach. The novel isolate was Gram-stain-negative, non-motile, aerobic and non-spore-forming. It was able to grow at 4–45 °C and pH 4.0–8.0, and exhibited NaCl tolerance of up to 1.5 % (w/v). Strain SYSU D8009T shared the closest 16S rRNA gene sequence similarities with members of the family Acetobacteraceae , with a value of less than 96.0 %. In the phylogenetic dendrograms, the strain clustered with the genera Paracraurococcus , Craurococcus and Crenalkalicoccus within the family Acetobacteraceae but with a distinct lineage, thereby demonstrating that the strain should be classified within the family Acetobacteraceae . The respiratory ubiquinone was found to be Q-10. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminolipids. The predominant cellular fatty acids were summed feature 8 (C1 8 : 1ω7c and/or C1 8 : 1ω6c) and C16 : 0. The genomic DNA G+C content of strain SYSU D8009T was determined to be 71.6 mol%. Based on the results of the phylogenetic analyses and differences in the physiological and biochemical characteristics, strain SYSU D8009T merits representation of a novel species of a new genus within the family Acetobacteraceae , for which the name Siccirubricoccus deserti gen. nov., sp. nov. is proposed. The type strain of Siccirubricoccus deserti sp. nov. is SYSU D8009T (=CGMCC 1.15936T=KCTC 62088T).
Eukaryotic Micro-organisms
Description of Dioszegia patagonica sp. nov., a novel carotenogenic yeast isolated from cold environments
Andrea Trochine, Benedetta Turchetti, Aline B. M. Vaz, Luciana Brandao, Luiz H. Rosa, Pietro Buzzini, Carlos Rosa and Diego Libkind
During a survey of carotenogenic yeasts from cold and oligotrophic environments in Patagonia, several yeasts of the genus Dioszegia (Tremellales, Agaricomycotina) were detected, including three strains that could not be assigned to any known taxa. Analyses of internal transcribed spacer and D1/D2 regions of the large subunit rRNA gene showed these strains are conspecific with several other strains found in the Italian Alps and in Antarctica soil. Phylogenetic analyses showed that 19 of these strains represent a novel yeast species of the genus Dioszegia. The name Dioszegia patagonica sp. nov. is proposed to accommodate these strains and CRUB 1147T (UFMG 195T=CBMAI 1564T=DBVPG 10618T=CBS 14901T; MycoBank MB 819782) was designated as the type strain. This Dioszegia species accumulates biotechnologically valuable compounds such as carotenoid pigments and mycosporines.
Jaminaea pallidilutea sp. nov. (Microstromatales), a basidiomycetous yeast isolated from plant material of mangrove forests in Iran
Shaghayegh Nasr, Mona Mohammadimehr, Marzieh Geranpayeh Vaghei, Mohammad Ali Amoozegar, Seyed Abolhassan Shahzadeh Fazeli and Andrey Yurkov
In the course of an ongoing study aiming to catalogue the natural yeast biodiversity of Iran, a number of yeasts were isolated from plant material collected from mangrove forests on the shoreline of Qeshm Island. Two strains were identified as members of order Microstromatales. Standard phenotypic, biochemical, physiological characterization and a phylogenetic analyses of the combined 26S rRNA gene (D1/D2 domains) and ITS region sequences showed the conspecificity of these isolates and suggest their placement in the genus Jaminaea, close to Jaminaea lanaiensis and Jaminaea angkoriensis. Here, we describe this species as Jaminiaea pallidilutea sp. nov. with IBRC-M 30284T=DSM 104392T=CBS 14684T as the type strain. The Mycobank accession number is MB 819618.
Heitmania gen. nov., a new yeast genus in Microbotryomycetes, and description of three novel species: Heitmania litseae sp. nov., Heitmania castanopsis sp. nov. and Heitmania elacocarpi sp. nov.
Xin-Zhan Liu, Marizeth Groenewald, Teun Boekhout and Feng-Yan Bai
Nine anamorphic yeast strains isolated from various plant leaves collected in southern China were phylogenetically characterized based on sequences of the internal transcribed spacer (ITS) region, the D1/D2 domains of the large subunit (LSU) rRNA gene, the small subunit (SSU) rRNA gene, the two subunits of the RNA polymerase II gene (RPB1 and RPB2) and the translation elongation factor 1-α (TEF1). Phylogenetic analysis of the combined sequences of the six genes showed that the new strains formed a distinct clade in the class Microbotryomycetes but could not be assigned to any of the existing genera, families or orders of the class. Three separate groups were consistently resolved from the nine new strains based on the combined sequences of the six genes and single gene sequences of ITS, RPB1, RPB2 and TEF1. The results suggest that the nine yeast strains compared represent three novel species in a novel genus. The names Heitmania gen. nov. (MycoBank registration number MB819987), Heitmania litseae sp. nov. (MB820112, type strain CGMCC 2.5697T=CBS 14756T), Heitmania castanopsis sp. nov. (MB819988, CGMCC 2.5698T=CBS 14750T) and Heitmania elacocarpi sp. nov. (MB820113, CGMCC 2.5695T=CBS 14752T) are proposed for the new taxa.
Kuraishia mediterranea sp. nov., a methanol-assimilating yeast species from olive oil and its sediment
Neža Čadež, Dénes Dlauchy, Andrea Tóbiás and Gábor Péter
Six yeast strains isolated from olive oil sediments and spoiled olive oils originating from Slovenia and Portugal, respectively, proved to represent an undescribed yeast species based on DNA sequence comparisons. The analysis of gene sequences for internal transcribed spacer regions and the large subunit rRNA gene D1/D2 domain placed the novel species in the genus Kuraishia in a subclade containing Kuraishia capsulata, the type species of the genus. Although the novel species is well separated genetically from the recognized species of the genus, only a minor phenotypic difference differentiating it from Kuraishia capsulata and K. molischiana was observed. Relevant to its isolation source, no lipolytic activity was detected in the strains of the novel species. To accommodate the above-noted strains, Kuraishia mediterranea sp. nov. (holotype: ZIM 2473T; isotype: CBS 15107T; MycoBank no.: MB 822817) is proposed.
Erratum: Rugosibacter aromaticivorans gen. nov., sp. nov., a bacterium within the family Rhodocyclaceae, isolated from contaminated soil, capable of degrading aromatic compounds
Elizabeth M. Corteselli, Michael D. Aitken and David R. Singleton
Corrigendum: Proposal to reclassify Propionibacterium acnes type I as Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov.
Andrew McDowell, Emma Barnard, Jared Liu, Huiying Li and Sheila Patrick
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Volumes and issues
content/journal/ijsem
Approved Lists of Bacterial Names
V. B. D. SKERMAN, VICKI McGOWAN and P. H. A. SNEATH
Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics
L. G. Wayne, D. J. Brenner, R. R. Colwell, P. A. D. Grimont, O. Kandler, M. I. Krichevsky, L. H. Moore, W. E. C. Moore, R. G. E. Murray, E. Stackebrandt, M. P. Starr and H. G. Truper
Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species
Ok-Sun Kim, Yong-Joon Cho, Kihyun Lee, Seok-Hwan Yoon, Mincheol Kim, Hyunsoo Na, Sang-Cheol Park, Yoon Seong Jeon, Jae-Hak Lee, Hana Yi, Sungho Won and Jongsik Chun
Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology
E. STACKEBRANDT and B. M. GOEBEL
Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography
MOSTAFA MESBAH, USHA PREMACHANDRAN and WILLIAM B. WHITMAN
Methods for characterization of Streptomyces species 1
E. B. Shirling and D. Gottlieb
Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains
TAKAYUKI EZAKI, YASUHIRO HASHIMOTO and EIKO YABUUCHI
EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences
Jongsik Chun, Jae-Hak Lee, Yoonyoung Jung, Myungjin Kim, Seil Kim, Byung Kwon Kim and Young-Woon Lim
DNA–DNA hybridization values and their relationship to whole-genome sequence similarities
Johan Goris, Konstantinos T. Konstantinidis, Joel A. Klappenbach, Tom Coenye, Peter Vandamme and James M. Tiedje
Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies
Seok-Hwan Yoon, Sung-Min Ha, Soonjae Kwon, Jeongmin Lim, Yeseul Kim, Hyungseok Seo and Jongsik Chun
Fleming Showcase
Join Sir Paul Nurse and celebrate the legacy of past Fleming winners and up-and-coming scientists at this special event on 30 March 2020, Edinburgh International Conference Centre.
Tweets by @MicrobioSoc
http://instance.metastore.ingenta.com/content/journal/ijsem/67/11


text:
Google's self-driving cars to hit roads, with steering wheels
By Paul Lienert
Reuters May 15, 2015
DETROIT (Reuters) - Google Inc will begin testing self-driving cars of its own design on public roads this summer, but they will have steering wheels and brakes, which is not what the company described a year ago.
Engineers will operate 25 prototype vehicles, which use the same software as Google's self-driving Lexus RX450h sport utility vehicles that have been in operation for several years, the company said on Friday.
When the Internet search company announced a year ago that it planned to build a fleet of self-driving cars, project director Chris Urmson said the prototypes "won't have a steering wheel, accelerator pedal or brake pedal ... because they don't need them."
It turns out, however, that the prototypes will need those critical control devices after all because California requires that self-driving vehicles have manual controls during testing.
Built in Detroit by auto supplier Roush Industries, the prototypes will be equipped with removable steering wheels, brake pedals and accelerator pedals so test engineers can "take over driving if needed," Urmson said in a blog post on Friday.
Google describes the pod-like prototype, which seats two people, as "the world's first fully self-driving vehicle."
The company also said it would test new passenger and pedestrian protection technologies, including a foam front end and a flexible windshield. It also said the speed of the prototypes will be limited to 25 mph (40 kph) to "decrease the likelihood of severe injury" in a collision.
As part of the rollout of the self-driving prototypes, Google said its engineers plan to test for "rare and weird situations" - what self-driving car proponents refer to as "edge cases," or unusual situations that occur once every 100,000 miles or so.
Such testing, Google said, will "help us validate that our software handles even the rarest variations on common driving scenarios."
On Monday, Google disclosed that its self-driving Lexus vehicles had been involved in 11 accidents on public roads near its Mountain View, California, headquarters.
"Not once was the self-driving car the cause of the accident," Urmson said in the blog post. No one was injured in the accidents, he added. The cars had been hit from behind seven times, mainly at traffic lights, with a majority of the accidents being on city streets rather than on freeways.
Google said its self-driving cars have been logging about 10,000 miles a week and have accumulated nearly 1 million miles.
Google shares were down 3.6 percent at $545.60 on Friday afternoon.
(Additional reporting by Yasmeen Abutaleb in Washington; Editing by Matthew Lewis)
Google Pixel 3 XL is on sale for $260 off for Prime Day
Waze has a new crowdsourced feature that will make navigation even better
BGR News
What to Do When Your Car Stalls
Asian shares mixed as China reports economy slowed in 2Q
3 Solar Stocks Set to Benefit From China's $247M Subsidies


The Montana Academy Foundation aspires to be a significant voice in a growing national conversation about character development in general and about delayed or interrupted maturity in particular. The Foundation pursues this aspiration through the publication of its newsletter, Sky Lines, through an annual conference, and through collaborative efforts with other organizations working in the field.
Sky Lines is distributed twice a year and provides updates on the Foundation's activities, along with articles and perspectives from professionals working in the field of adolescent mental health and from members of the extended Montana Academy family.
If you would like to be added to the mailing list for Sky Lines, please click here.
If you would like to access the archive of past issues of Sky Lines, please click here.
On October 12-13, 2017, the Montana Academy Foundation sponsored the first of a series of conferences on Immaturity, Character Development, and Treatment of Adolescents.
This first conference was held at the Grouse Mountain Lodge in Whitefish, MT, and was attended by more than 70 mental health professionals. Distinguished practitioners in the field of adolescent mental and behavioral health addressed central issues related to attachment disorders in adolescents.
The Foundation will soon announce the dates of a second conference. This meeting will focus on "Teenage Anxiety: Symptoms, Treatments, and Root Causes." Please check back to the "What's New" section of our Home page for further information.


The parents of a 2-year-old Vacaville girl who was accidentally shot to death by her 8-year-old brother have been convicted of criminal charges, an attorney in the case said Friday.
Michael Shanahan, 28, and Daniela Shanahan, 26, could each face up to two years in prison in connection with the Sept. 23 death of Ayana Shanahan.
Michael Shanahan pleaded no contest Thursday in Solano County Superior Court to one count of unsafe storage of a firearm. Daniela Shanahan pleaded guilty to one count of child endangerment.
Prosecutors are seeking two-year prison terms when both are sentenced April 30. But defense attorneys will argue for probation on the grounds that the parents have already suffered enough, said Michael Shanahan's lawyer, Anthony Finkas.
"Obviously, they are devastated by what happened," Finkas said. "They're serving a life sentence based on their apparent negligence."
Ayana was shot once in the head at the family's home on the 1000 block of Syracuse Circle as her brother was playing with a handgun.
The boy did not think the gun was real, police said. The parents were home at the time.
Michael Shanahan was an active Army reservist at the time of the shooting, and the gun that killed Ayana was one of numerous weapons in the home, authorities said.


text:
The countdown is started : Seals on their way to the Magdalen Islands!
The countdown is officially started with 2 days left before the 2014 seal watching season. More than one hundred guests will travel from around the world to come to this annual meeting with the whitecoats, unique world phenomenon. This year, the weather conditions are just perfect and everything is in place to have one of the greatest season in the past 10 years. The seal herd had already been located at about 46 miles on the North shore of L'Étang-du-Nord.
A first in the history of the Château Madelinot, the hotel will welcome their first chinese guests! "It's very fascinating to see on the flight ticket of a guest: Beijing- Magdalen Islands", to say Ariane Bérubé, Sales Manager at Hôtels Accents. "We are proud to contribute to the promotion of the destination as far as in China", to add Mrs Bérubé. Faithful since 1986, the japanese guests will again take part of the seal watching expeditions. Will also join this unique winter experience, guests from France, Germany, Holland, Australia, Belgium, Switzerland, United States and Canada.
"Launch in January with the Ice Hotel of Québec, the package «Winter Adventure» receive a very positive welcome which let us confident for the years to come", to say Gino Thorne, Director at Hôtels Accents. We are proud to take part of the «Quebec Original» campaign and to contribute at the development of the Quebec destination. "This unique adventure also brings an important spotlight on our destination, Magdalen Islands and all the Québec Maritime region", to add Mr Thorne. New this year, people will be able to follow the 2014 seal watching season on Facebook, Twitter and Instagram.
Follow the 2014 Seal watching season :
Facebook : www.facebook.com/hotelsaccents
Twitter: @Hotelsaccents #sealwatching
Instagram : @hotelsaccents #sealwatching
Web : www.blanchons.com
Ariane Bérubé
Hôtels Accents
97,114,105,97,110,101,46,98,101,114,117,98,101,64,104,111,116,101,108,115,97,99,99,101,110,116,115,46,99,111,109|97,114,105,97,110,101,46,98,101,114,117,98,101,64,104,111,116,101,108,115,97,99,99,101,110,116,115,46,99,111,109,60,47,97,62|Courriel via www.tourismeilesdelamadeleine.com|||
www.hotelsaccents.com


text:
KVJY (840 AM) is a radio station licensed to Pharr, Texas.  The station airs a Spanish Christian format.

In May 2017, Bi-Media, composed of members of the Bichara family which also owns radio holdings in Mexico, sold KVJY to Daij Media. Daij is owned by the Villarreal family and primarily operates Radio Aleluya religious stations. The sale, at a price of $910,000, was consummated on July 18, 2017.

References

External links

News and talk radio stations in the United States
VJY
Radio stations established in 1989
1989 establishments in Texas

redpajama_set_name: RedPajamaWikipedia


text:
Rhythm & Views
Preston School of Industry
By Stephen Seigel
Preston School of Industry performs at Club Congress, 311 E. Congress St., on Wednesday, June 19. The show begins at 9 p.m. with opening sets by David Dondero and The Gaylords. Admission is $8. For more information call 622-8848.
It's often difficult to remember that Scott Kannberg was once officially half of Pavement, one of the finest and most influential smart(y-pants) rock bands of the '90s ('89 to '00, to be exact). Kannberg--or, if you prefer, Spiral Stairs, his nom de musique--was gradually reduced to playing second fiddle to fellow singer/guitarist/songwriter Stephen Malkmus, until the band's final album, 1999's Terror Twilight (on Matador, as are all the band's full-length releases, save an odds 'n' sods collection on Drag City), which included not a single Kannberg contribution; his compositions were, instead, relegated to b-side status by Malkmus, who had finally completely edged his way into "de facto" bandleader. Pity Kannberg, though, as he was competing with one of the most literate and tuneful songwriters of the decade in Malkmus. Still, Kannberg was no slouch himself, having written a handful of old reliables from the Pavement canon, "Forklift" and "Two States," from the band's groundbreaking debut LP, Slanted and Enchanted ('92), among them. Then again, he wrote "Hit the Plane Down," too.
In the beginning, though, things were different. In the beginning, Pavement was two buddies on equal footing, screwing around in a garage studio, who just happened to create a self-released seven-inch single that got into the right hands, influential British DJ John Peel among them. Pavement became a highly accidental and successful career in pretty short order.
But success breeds egos, which get both large and bruised, even in the self-righteous world of indie-rock, and rumor has it there was no love lost between the two songwriters when Pavement finally, quietly, cryptically (as was its way all along, from the shadowy monikers to the self-perpetuated rumors that the band had gotten in a brawl with the cast of 90210) disbanded.
Last year bore the first wave of solo records from both, with Malkmus releasing an eponymous album under his actual name (albeit with the Jicks, his backing band), and Kannberg opting for Preston School of Industry (named after a reform school once attended by Merle Haggard), which debuted with All This Sounds Gas (both were released on Matador). A perusal of Kannberg's bio sheet lets you know that, while he's extremely proud of what he did in Pavement (the press kit is quick to point out that, after recording Pavement's debut single, Malkmus, who is cheekily referred to throughout only as Kannberg's unnamed "childhood friend," "[mysteriously] disappears with backpack into post-graduation Europe, [while] Scott toils half-heartedly" at a local record store and selflessly works to distribute the 7-inch), he's also eager to leave it all behind.
Where Malkmus' solo album basically streamlined his approach to the crooked pop song, highlighting the narrative instead of making it obtuse (for which he was often criticized in Pavement), Kannberg leaves in all the sonic weirdness that was a part of Pavement's allure from the get-go. Both are exceptional guitarists, always forsaking technique and accuracy for tastefulness and passion (indeed, it was often difficult to recognize which guitarist--Kannberg or Malkmus--was playing what until one saw them perform live), and the torch of the "Pavement sound" is well intact in Kannberg's hands. And while it may be true that no one ever bought a post-Slanted Pavement album for the Spiral Stairs songs, All This Sounds Gas comes off like a compilation of lost Pavement-era Kannberg songs, save album-closing epic "Take a Stand," which clocks in at a cool 8:17, and puts Lennon-esque reverb on the vocals, which are backed by a grandiose orchestral pop arrangement and sha-la-la-la's straight from heaven.
Stephen Seigel
If You Don't Have Plans To Go Out Tonight: Fox Tucson Theater Presents "Friday Nights In"
By Tara Foulkrod
KFMA Day Has Been Canceled Amid COVID-19 Closures
Things to Do, Wednesday, Feb. 19
By TW Fun & Games Desk
Latest in Rhythm & Views
By Eric Swedlund
By Joshua Levine
Shaun Harris "Journey to F-Sides" (Self-Released)
More Rhythm & Views »


All your invoices and other email notifications will be sent to your nominated email address on your account. If you would like to change your email address on your Rocketspark account, you can do this here. If you are wanting just your invoices to go to a separate email address, called a billing email address, then the following steps are needed.
Login to your Rocketspark dashboard using your login details.
On the left hand menu click 'My Account'.
On the right hand side of your account details there is an 'Optional Billing Email' section. Your current email address will be automatically listed in this field as a default (or you may find that this space is blank - either way, it doesn't matter).
Delete the current email address and replace it with your new elected billing email address. Click the 'Save' button then click 'Ok'. Your new billing email address has now been updated and saved to your account. The next time you're invoiced, this new email will be used.
TIP: If you find that you'd like to add more than one email address (perhaps send a copy to your accounts team as well as yourself), you can. Just add a comma ( , ) after the first email address and then add the next.


GO BUILD that STOCKPILE!!! Quilted Northern Toilet Paper -- 13¢! STOCK UP Price!!!
GO BUILD that STOCKPILE!!! Quilted Northern Toilet Paper — 13¢! STOCK UP Price!!!
GO GO GO!!! If you find this… STOCK UP!!!
PLUS.. Use the $0.50/1 Quilted Northern Ultra Soft & Strong Bath Tissue, 6 double roll+ Coupon from the 3/19 RP to pay only $7.64 or 13¢ per regular roll! WOW!!!
Look for SKU # 253-06-0136 to find this item.


The National Committee for Anti-Human Trafficking yesterday unveiled its plan to seek bail for 32 pregnant women who were charged with human trafficking following a raid on a surrogacy business in the capital in June.
Its chairwoman Chou Bun Eng said after a meeting at the Interior Ministry yesterday that a group of lawyers are currently preparing documents to submit to a judge to seek bail for the women.
"Some women usually claim that they get involved in the surrogacy business because they need money to pay debts. The judge will ask them if they will sell the babies to earn money after birth. This is a factor to consider," Ms Bun Eng added.
She noted that the National Committee for Anti-Human Trafficking offered legal assistance for the bail requests in order to ensure that the surrogate children are taken care of.
Phnom Penh Municipal Court spokesman Ly Sophanna could not be reached for comment.
The 32 women were arrested when police raided a house in Russey Keo district on June 21. They were charged with human trafficking along with five members of a surrogacy ring.
Ms Bun Eng noted that the pregnant women are currently receiving treatment at a hospital under police supervision and that Agape International Missions is assisting them.
Agape International Missions CEO Don Brewster could not be reached for comment yesterday.
An official at AIM, however, confirmed that the organisation provided a group of lawyers to work with the National Committee for Anti-Human Trafficking in an effort to seek bail for the 32 women.
According to a police report, the 32 women were hired by the ring's leader and promised $10,000 to carry the babies. They were given $500 cash up front and then $300 per month while carrying their babies, the report said.
After delivery, they were to get the remaining $6,000 from the ring's leader, who was operating the scheme under the business name Fertility Solutions PGD.


Let's look beyond the electricity crisis and join us to discover alternative options to ensure your business keeps running -with the lights on!
Did you know that on 1st of April, the price of electricity increased by 14%? Last month, the National Energy Regulator of South Africa (NERSA) granted Eskom consecutive increase allowances amounting to 23% by 2022. Eskom is facing a debt now exceeding R420 Billion and one may expect more loadshedding over the next year as the situation will take time to improve.
What can we expect over the next 6 months? What are the risks with the loadshedding for SME's? Generators, batteries, Solar systems... What could be the alternatives and for what ROI?
Jacques Van Der Merwe (SOLARTECH) is an accredited service provider for City of Cape Town and is an energy specialist for green alternatives such as solar systems. Jacques will present us the Eskom situation and the potential alternatives during 40 minutes and will also answer your questions.
No parking provided, parking along the road, we recommend using Uber.
A light breakfast will be served (hot/cold drinks and croissants).
Register here or with the above register button.


Occupation Freedom: Movement Music the Album is Now Available!
Powerful, Raw, and Inspirational that Captures the Original Spirit of Activism, Love and Hopefulness at the core of Hip Hop Culture and Peoples Movements Everywhere.
This is Movement Music. This is Hip Hop !
The album is now available on all the major corporate sites like Itunes, etc... but also through our Independent Virtual Store Front! We encourage that of course.
Thank you for joining in the celebration and lets go!
Here are videos for the first two singles from the album songs that helped to create the soundtrack for the global convergence movement known as Occupy Wall Street.


While this speech certainly would not be the deciding factor in whom I ultimately vote for, it does open the door for more thoughtful consideration.
For the African-American community, that path means embracing the burdens of our past without becoming victims of our past. It means continuing to insist on a full measure of justice in every aspect of American life. But it also means binding our particular grievances – for better health care, and better schools, and better jobs – to the larger aspirations of all Americans — the white woman struggling to break the glass ceiling, the white man who's been laid off, the immigrant trying to feed his family. And it means taking full responsibility for own lives – by demanding more from our fathers, and spending more time with our children, and reading to them, and teaching them that while they may face challenges and discrimination in their own lives, they must never succumb to despair or cynicism; they must always believe that they can write their own destiny.
Obama is digging his own political grave. It has been about 2 weeks since this story about the openly racist Rev. J. Wright and Obama still tries to find excuses for this man. Obama is probably not racist, but he certainly made a racial statement regarding his grandmother. In a radio show, Obama stated, "She is a typical white woman…" trying to clarify the comments he made about his white grandmother in his recent speech about race. This audio clip will haunt him for the rest of his doomed campaign. He has aliented many potential voters for him in November. Senator Obama will stay a senator. He cannot be elected president.
For Obama to have any chance for the presidency, he will have to completely sever ties with Rev. Wright. He will not do this because he will also sever ties with black voters that support Rev. Wright. This is his Achille's heel. How could any candidate for the presidency be a member of a church that believes the following?: "We are a congregation which is unashamedly black and unapologetically Christian," says the Trinity United Church of Christ's Web site. Imagine McCain belonging to a church that had a statement stating – "We are a congregation which is unashamedly white and unapologetically Christian." In this hypothetical, McCain would be doomed and his entire political career would be over because the mainstream media would annihilate him and they would be right to do so.
Rev. Wright has said, "U.S. of K.K.K.A." and other things that are offensive, hateful, and thoughtless. How Obama could have spent almost 2 decades attending a church like that is incredible. Are American citizens really supposed to believe that Obama has never agreed with any of Rev. Wright's distorted, racist viewpoints? Perhaps he does not condone any of the Reverend's outrageous statements, but in politics that does not matter. Perception is everything. A great deal of potential supporters will not give Obama their vote after hearing this vitriolic diatribe from his close spiritual advisor.
About one year ago, Rev. Wright spoke with Obama and said that he would keep a low profile during Obama's campaign. Even Rev. Wright was aware that some of his sermons would jeopordize Obama's chance to be the Democratic candidate for president. Apparently, Obama did not understand it! This is probably the only piece of sensible advice that Obama should have taken from his reverend. He will finally understand it in November, when he loses.


Laser Quantum has recently been included in the London Stock Exchange list as one of the 1000 companies across the UK to inspire Britain. The prestigious report has included Laser Quantum as one of the 42 businesses to be listed from Greater Manchester. This acclamation supports the entrepreneurial nature of Laser Quantum and the North West.
Being part of this list is a reflection of Laser Quantum's attitudes, as these companies are chosen because they highlight dynamic, customer focused and ambitious companies, assets Laser Quantum incorporates into its strategy.
To read the full article, please follow this link to Manchester Evening News.