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Extracellular Electron Transfer: Respiratory or nutrient homeostasis?
Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens like Geobacter sulfurreducens and Shewanella oneindensis are Gram-negative, but more recently it has been reported that Gram-positive bacteria like Listeria monocytogenes and Enterococcus faecalis also exhibit the ability to transfer...
Bacillus subtilis Fur is a transcriptional activator for the PerR-repressed pfeT gene encoding an iron efflux pump
The physiological relevance of bacterial iron efflux has only recently been appreciated. The Bacillus subtilis P1B4-type ATPase PfeT (peroxide-induced ferrous efflux transporter) was one of the first iron efflux pumps to be characterized, and cells lacking pfeT accumulate high levels of intracellular iron. The pfeT promoter region has binding sites for both PerR, a peroxide sensing Fur-family...
Diverse and Flexible Transmission of fosA3 Associated with Heterogeneous Multidrug Resistance Regions in Salmonella enterica Serovar Typhimurium and Indiana Isolates
We identified fosA3 at a rate of 2.6% in 310 Salmonella isolates from food animals in Guangdong province, China. The fosA3 gene was genetically linked to diverse antibiotic resistance genes (ARGs), including mcr-1, blaCTX-M-14/55, oqxAB, and rmtB. These gene combinations were embedded in heterogeneous fosA3-containing multidrug resistance regions on the transferable ST3-IncHI2 and F33:A–:B...
In Vitro Activity of Plazomicin Compared to Amikacin, Gentamicin, and Tobramycin against Multidrug-Resistant Aerobic Gram-Negative Bacilli
The worldwide spread of multidrug-resistant Enterobacterales is a serious threat to public health. Here, we compared the MICs of plazomicin, amikacin, gentamicin, and tobramycin against 303 multinational multidrug-resistant Gram-negative bacilli. We followed Clinical and Laboratory Standards Institute (CLSI) guidelines and applied CLSI breakpoints as well as those of the European Committee on...
High Heterogeneity of Multidrug-Resistant Enterobacteriaceae Fecal Levels in Hospitalized Patients Is Partially Driven by Intravenous {beta}-Lactams
Multidrug-resistant Enterobacteriaceae (MRE) colonize the intestine asymptomatically from where they can breach into the bloodstream and cause life-threatening infections, especially in heavily colonized patients. Despite the clinical relevance of MRE colonization levels, we know little about how they vary in hospitalized patients and the clinical factors that determine those levels. Here, we...
In Vitro Susceptibility Testing of GSK656 against Mycobacterium Species
In this study, we aimed to assess the in vitro susceptibility to GSK656 among multiple mycobacterial species and to investigate the correlation between leucyl-tRNA synthetase (LeuRS) sequence variations and in vitro susceptibility to GSK656 among mycobacterial species. A total of 187 mycobacterial isolates, comprising 105 Mycobacterium tuberculosis isolates and 82 nontuberculous mycobacteria (NTM...
Evaluation of chlorine dioxide gas release rates from dry precursors intended for applied technologies under disparate conditions and their effects on Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes
Innovative Food Science & Emerging Technologies
Author(s): David Buckley, Bassam A. Annous, Bryan VinyardAbstractThe diverse food safety issues facing developed countries require innovations to current food safety intervention technologies and chemistries. Several innovations have been made to highly efficacious treatments, such as chlorine dioxide (ClO2). However, many of the innovations stop short of evaluating actual yield, practical...
Prevalence and Antibiotic Resistance Profiles of Staphylococcus Spp Isolated From Food, Food Contact Surfaces and Food Handlers in a Moroccan Hospital Kitchen
Letters in Applied Microbiology
Food poisoning risk related to the consumption of contaminated food with known foodborne pathogens or antibiotic‐resistant bacteria is currently a serious threat for public health. Thus, pathogenic Methicillin‐resistant Staphylococcus strains are considered as one of the major cause of food‐borne diseases in hospitals. The present study aims to determine the prevalence and the antibiotic...
Aerobic industrial processing of Empeltre cv. natural black olives and product characterisation
International Journal of Food Science & Technology
Industrial aerobic fermentation of natural black olives. Summary The fermentation of natural black olives is currently carried out under anaerobic conditions. This work investigates the aerobic fermentation of this product in tanks containing 16 000 kg of Empeltre olives during two seasons. The microorganisms in brines were yeasts in all cases, whereas lactic acid bacteria growth was only...
Quantitative detection of viable but nonculturable state Escherichia coli O157:H7 by ddPCR combined with propidium monoazide
Author(s): Hanxu Pan, Kai Dong, Lei Rao, Liang Zhao, Xiaomeng Wu, Yongtao Wang, Xiaojun LiaoAbstractEscherichia coli O157:H7, the causative agent of haemorrhagic colitis and haemolytic uremic syndrome in humans, has been implicated in large food-borne outbreaks all over the world. When confronted with harsh environmental stresses, it can enter into a viable but nonculturable (VBNC) state, which...
Shotgun Proteomic Analysis of ESBL-Producing and Non-ESBL-Producing Klebsiella Pneumoniae Clinical Isolates
Author(s): Shymaa Enany, Samira Zakeer, Ahmed A. SayedAbstractKlebsiella pneumoniae is a pathogenic bacterium that is responsible for a wide range of infections in humans. An increased rate of infections caused by multi-drug-resistant K. pneumoniae has been noted in the last two decades. The association between antimicrobial resistance and virulence is an important topic of study. Genomic tools...
Whole genome sequencing in drug susceptibility testing of Mycobacterium tuberculosis in routine practice in Lyon, France
International Journal of Antimicrobial Agents
Author(s): Charlotte Genestet, Elisabeth Hodille, Jean-Luc Berland, Christophe Ginevra, Juliet E. Bryant, Florence Ader, Gérard Lina, Oana Dumitrescu, Lyon TB study groupAbstractObjectivesRapid and correct determination of Mycobacterium tuberculosis (MTB) drug-susceptibility is a challenge for tuberculosis management. Phenotypic drug-susceptibility testing (DST) remains the reference method but...
Antimicrobial production by strictly anaerobic Clostridium species
Author(s): Amila Srilal Nawarathna Weligala Pahalagedara, Steve Flint, Jon Palmer, Gale Brightwell, Tanushree Barua GuptaAbstractAntimicrobial resistance continues to grow on a global scale affecting environment, humans, animals and food systems. The use of natural antimicrobials has been favoured over synthetic molecules in food preservation due to concerns over adverse health effects of...
Polyamine depletion has global effects on stress and virulence gene expression and affects HilA translation in Salmonella enterica serovar Typhimurium
Research in Microbiology
Author(s): Priscila R. Guerra, Gang Liu, Sebastien Lemire, Arkadiusz Nawrockic, Egle Kudirkiene, Jakob Møller-Jensen, John E. Olsen, Lotte JelsbakAbstractPolyamines are small cationic amines required for modulating multiple cell process, including cell growth and DNA and RNA stability. In Salmonella polyamines are primarily synthesized from L-arginine or L-ornithine. Based on a previous study,...
Chitosan-starch film containing pomegranate peel extract and Thymus kotschyanus essential oil can prolong the shelf life of beef
Meat Science
Author(s): Tooraj Mehdizadeh, Hossein Tajik, Ali Mojaddar Langroodi, Rahim Molaei, Alireza MahmoudianAbstractThe aim of the current study was to investigate the effects of pomegranate peel extract (PPE) and chitosan-starch (CH-S) composite film incorporated with Thymus kotschyanus essential oil (TEO) on the shelf-life of beef during storage period of 21 days at 4 °C. The physico-mechanical...
Q fever in Greece: Findings of a 13 years surveillance study
Comparative Immunology, Microbiology and Infectious Diseases
Author(s): Anna PsaroulakiAbstractQ fever is an endemic disease in different parts of Greece. The current study aimed to investigate the prevalence of acute Q fever disease in Greece through the operation of the national reference centre for Q fever.A total of 5397 sera were received from febrile patients under the suspicion of Q fever infection during a 13 years period (2001-20013). A...
Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese
International Dairy Journal
Author(s): Mahmoud Ge. Zayda, Yoshimitsu Masuda, Ahmed M. Hammad, Ken-ichi Honjoh, Abdelrahman M. Elbagory, Takahisa MiyamotoAbstractWe investigated the characteristics of S. aureus isolates causing bovine subclinical mastitis (SCM), and their genetic relatedness with the isolates obtained from Egyptian cheese. Twenty-five S. aureus isolates were identified from 150 SCM milk and 75 cheese samples...
Expression of the hybrid bacteriocin Ent35-MccV in Lactococcus lactis and its use for controlling Listeria monocytogenes and Escherichia coli in milk
Author(s): Leonardo Acuña, Natalia Corbalan, Marcos Quintela-Baluja, Jorge Barros-Velázquez, Augusto BellomioAbstractBacteriocins are antimicrobial peptides synthesised ribosomally. One of the main drawbacks of bacteriocins as food biopreservative agents is their restricted action spectrum. To obtain a broad-spectrum bacteriocin-producing lactic acid bacterium, we engineered a fusion protein...
Antimicrobial resistance phenotypes and genotypes of methicillin-resistant Staphylococcus aureus CC398 isolates from Spanish hospitals
Author(s): Sara Ceballos, Carmen Aspiroz, Laura Ruiz-Ripa, Myriam Zarazaga, Carmen Torres, Spanish study group on clinical LA-MRSAAbstractLivestock-associated (LA) methicillin-resistant Staphyloccoccus aureus (MRSA) of lineage CC398 is an emerging clone causing human infections, although they are most commonly found in pigs. The aim of this study was to characterize the antimicrobial resistance...
Non-S. aureus staphylococci (NAS) in milk samples: Infection or contamination?
Veterinary Microbiology
Author(s): Johannes Hamel, Yanchao Zhang, Nicole Wente, Volker KrömkerAbstractNon-S. aureus staphylococci (NAS) are the most frequently isolated pathogens from bovine milk and can cause intramammary infections (IMI). They can also be found in teat canals, on bovine skin and in cows' environment, which may lead to unnoticed contamination of milk samples. The aim of this study was to investigate...
YERSINIA ENTEROCOLITICA-SPECIFIC MODULATION OF INNATE IMMUNE RESPONSES IN JEJUNAL EPITHELIAL CELLS
Author(s): Elisabetta Razzuoli, Walter Vencia, Paola Modesto, Giulia Franzoni, Silvia Dei Giudici, Erica Parisi, Angelo Ferrari, Massimo AmadoriAbstractGut is often subject to infection by different pathogens like Y. enterocolitica. To date, biotypes (BTs) 1A have been considered as non-pathogenic, because they do not express plasmid of virulence pYV; however, BTs 1A strains present other...
Effectiveness of a peracetic acid solution on Escherichia coli reduction during fresh-cut lettuce processing at the laboratory and industrial scales
International Journal of Food Microbiology
Author(s): J.L. Banach, H. van Bokhorst-van de Veen, L.S. van Overbeek, P.S. van der Zouwen, M.H. Zwietering, H.J. van der Fels-KlerxAbstractFresh leafy greens like lettuce can be consumed raw and are susceptible to food-borne pathogens if they become contaminated. Recently, the number of reported pathogenic foodborne outbreaks related to leafy greens has increased. Therefore, it is important to...
Microbial safety of cheese in Canada
Author(s): Kyle Ganz, Etsuko Yamamoto, Kate Hardie, Christine Hum, Hussein Hussein, Annie Locas, Marina SteeleAbstractA profile of the microbial safety of cheese in Canada was established based on the analysis of 2955 pasteurized and raw-milk cheeses tested under Canada's National Microbiological Monitoring Program (NMMP) and 2009 raw-milk cheeses tested under the Targeted Survey Program. 97.8%...
Challenges in the management of anticoagulated patients with focal spasticity
Toxicon
Author(s): Chris Boulias, Farooq Ismail, Chetan P. PhadkeAbstractIntroductionIn patients taking warfarin, the level of anticoagulation status using the International Normalized Ratio (INR) is one consideration before botulinum toxin type-A (BoNTA) injections; however, there is wide diversity in physician approaches and preferences in using INR status and the INR cut-off values to determine...
Strategy for Starting BoNT Treatment (written consent, initial dose and subsequent doses, interval, follow-up)
Author(s): Joseph K.C. TsuiAbstractBotulinum toxin (BoNT) has become a therapeutic agent for a large variety of medical conditions. It is a symptomatic treatment and in most cases requires repeat injections at regular intervals. The first session is crucial in establishing rapport between the physician and the patient to ensure a continuity of treatment on a long term basis. Since the clinical...
HtrA‐dependent adherence and invasion of Campylobacter jejuni in human vs. avian cells
The aim of this study was to investigate if HtrA is responsible for differences in adherence and invasion of Campylobacter jejuni towards human and chicken cell lines. Gentamicin protection assays were performed with either human Caco‐2 or chicken 2G4 cells using C. jejuni strain NCTC11168 to compare the adhesion and invasion rates towards these two cell types. The results revealed significant...
Detection of antibiotic resistance and virulence genes of Vibrio strains isolated from ready‐to‐eat shrimps in Delta and Edo States, Nigeria
Journal of Applied Microbiology
Aim The current study was conducted to determine the incidence, antibiotic resistance and virulence genes of Vibrio strains isolated from ready‐to‐eat shrimps in Edo and Delta States, Nigeria. Methods and Results A total of 1440 ready‐to‐eat shrimp's samples were obtained from open markets from November 2016 to October 2017 and analysed using standard culture‐based procedures. Overall, our result...
Validation Software Tool (ValT) for Predictive Microbiology Based on the Acceptable Prediction Zones Method
A study was undertaken to develop a user‐friendly, software tool (ValT) for validation of predictive models based on the acceptable prediction zones (APZ) method. ValT was developed in Excel and was demonstrated using a newly developed growth model for Salmonella and chicken skin. The model provided acceptable predictions when the proportion (p) of residuals in the APZ was ≥ 0.70 and there were...
SRL pathogenicity island contributes to the metabolism of D-aspartate via an aspartate racemase in Shigella flexneri YSH6000
by Tania Henríquez, Juan Carlos Salazar, Massimiliano Marvasi, Ajit Shah, Gino Corsini, Cecilia S. Toro In recent years, multidrug resistance of Shigella strains associated with genetic elements like pathogenicity islands, have become a public health problem. The Shigella resistance locus pathogenicity island (SRL PAI) of S. flexneri 2a harbors a 16Kbp region that contributes to the multidrug...
Dominant negative effects by inactive Spa47 mutants inhibit T3SS function and Shigella virulence
by Jamie L. Burgess, Heather B. Case, R. Alan Burgess, Nicholas E. Dickenson Type three secretion systems (T3SS) are complex nano-machines that evolved to inject bacterial effector proteins directly into the cytoplasm of eukaryotic cells. Many high-priority human pathogens rely on one or more T3SSs to cause disease and evade host immune responses, underscoring the need to better understand the...
Susceptibility of foodborne pathogens to sanitizers in produce rinse water and potential induction of viable but non-culturable state
Author(s): Ganyu Gu, Samantha Bolten, Joseph Mowery, Yaguang Luo, Connor Gulbronson, Xiangwu NouAbstractChemical sanitizers are commonly used for fresh produce washing to reduce potential presence of foodborne pathogens and spoilage bacteria, and to prevent cross-contamination. In this study, we evaluated the susceptibility of foodborne pathogens, including Shiga-toxigenic Escherichia coli,...
Analysis of Neutral Electrolyzed Water anti-bacterial activity on contaminated eggshells with Salmonella enterica or Escherichia coli
Author(s): Jocelyn Medina-Gudiño, Andres Rivera-Garcia, Liliana Santos-Ferro, Juan C. Ramirez-Orejel, Lourdes T. Agredano-Moreno, Luis F. Jimenez-Garcia, David Paez-Esquiliano, Sandra Martinez-Vidal, Eduardo Andrade-Esquivel, Jose A. Cano-BuendiaAbstractNeutral Electrolyzed Water (NEW) was tested in vitro and on artificially contaminated eggs against Salmonella enterica subsp. enterica or...
Inhibition of germination and outgrowth of Clostridium perfringens spores by buffered calcium, potassium and sodium citrates in cured and non-cured injected pork during cooling
Author(s): Padmanabha Reddy Velugoti, Saurabh Kumar, Lalit Kumar Bohra, Vijay K. Juneja, Harshavardhan ThippareddiAbstractInhibition of Clostridium perfringens spore germination and outgrowth by buffered calcium, potassium, and sodium citrates (Ca-Cit, K-Cit, and Na-Cit, respectively) during exponential cooling of non-cured and cured pork was evaluated. Antimicrobials were added to a base...
Gut metabolite, Urolithin A decreases Actin Polymerization and Migration in Cancer Cells
Molecular Nutrition & Food Research
Scope Urolithin A (UA) is a gut‐derived metabolite from ellagic acid found in pomegranates, berries and nuts can down‐regulate cell proliferation and migration. Cell proliferation and cell motility require actin reorganization which is under control of ras‐related C3 botulinum toxin substrate 1 (Rac1) and p21 protein‐activated kinase 1 (PAK1). The present study explored whether Urolithin A can...
Feeling the heat at the millennium: thermosensors playing with fire
Many pathogenic bacteria use different types of thermosensors to sense increased temperature once inside the host. In this review, we are describing the identification of protein, DNA and RNA thermosensors that took place around 20 years ago. In particular, we describe the events leading to the discovery of the prfA thermosensor in Listeria monocytogenes (depicted). Abstract An outstanding...
Structure and function of Listeria teichoic acids and their implications
Teichoic acids are a diverse class of molecules on the surface of Gram‐positive bacteria known to play important roles in fitness, virulence and bacteriophage susceptibility. A significant level of structural and constitutional heterogeneity exists within the Listeria genus, and it is this variation which serves as a basis for serotyping. Here, we elaborate on recent findings in the Listeria...
Dissemination and conservation of cadmium and arsenic resistance determinants in Listeria and other Gram‐positive bacteria
While exposure to high levels of toxic metals such as cadmium and arsenic is only experienced in a handful of special habitats and ecosystems, Listeria and other Gram‐positive bacteria have evolved an impressively diverse array of genetic tools for acquiring enhanced tolerance to such metals. Here we summarize this fascinating collection of resistance determinants in Listeria monocytogenes and...
Development of a Sensitive Escherichia coli Bioreporter Without Antibiotic Markers for Detecting Bioavailable Copper in Water Environments
Frontiers in Microbiology
The whole-cell bioreporters based on the cop-operon sensing elements have been proven specifically useful in the assessment of bioavailable copper ions in water environments. In this study, a series of experiments was conducted to further improve the sensitivity and robustness of bioreporters. First, an Escherichia coli △copA△cueO△cusA mutant with three copper transport genes knocked out was...
Salmonella enterica Serovar Typhimurium 14028s Genomic Regions Required for Colonization of Lettuce Leaves
Contamination of edible produce leaves with human bacterial pathogens has been associated with serious disease outbreaks and has become a major public health concern affecting all aspects of the market, from farmers to consumers. While pathogen populations residing on the surface of ready-to-eat produce can be potentially removed through thorough washing, there is no disinfection technology...
Adult Intestinal Toxemia Botulism
Intoxication with botulinum neurotoxin can occur through various routes. Foodborne botulism results after consumption of food in which botulinum neurotoxin-producing clostridia (i.e., Clostridium botulinum or strains of Clostridium butyricum type E or Clostridium baratii type F) have replicated and produced botulinum neurotoxin. Infection of a wound with C. botulinum and in situ production of...
Molecular Epidemiology of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus in Wild, Captive and Laboratory Rats: Effect of Habitat on the Nasal S. aureus Population
Rats are a reservoir of human- and livestock-associated methicillin-resistant Staphylococcus aureus (MRSA). However, the composition of the natural S. aureus population in wild and laboratory rats is largely unknown. Here, 144 nasal S. aureus isolates from free-living wild rats, captive wild rats and laboratory rats were genotyped and profiled for antibiotic resistances and human-specific...
Mobilizable antibiotic resistance genes are present in dust microbial communities
by Sarah Ben Maamar, Adam J. Glawe, Taylor K. Brown, Nancy Hellgeth, Jinglin Hu, Ji-Ping Wang, Curtis Huttenhower, Erica M. Hartmann The decades-long global trend of urbanization has led to a population that spends increasing amounts of time indoors. Exposure to microbes in buildings, and specifically in dust, is thus also increasing, and has been linked to various health outcomes and to...
Draft Genome Sequence of Clostridium perfringens Strain TAMU, Which Causes Necrotic Enteritis in Broiler Chickens
Clostridium perfringens causes severe gastrointestinal diseases, which include necrotic enteritis (NE) in chickens, a deadly disease worldwide. We report here the draft genome sequence of Clostridium perfringens strain TAMU, which was used in developing an NE chicken challenge model. This C. perfringens TAMU genome sequence will aid in advancing potential intervention strategies to reduce NE...
Complete Genome Sequence and Annotation of Campylobacter jejuni YH003, Isolated from Retail Chicken
The complete genome sequence of Campylobacter jejuni YH003, isolated from retail chicken, was determined using PacBio and Illumina technologies. The assembled genome is 1,743,985 bp (G+C content of 30.3%). Genome annotation revealed several genes encoding virulence and antibiotic resistance factors, including a type VI secretion system, cytolethal distending toxins, and a multidrug efflux system.
Litopenaeus vannamei sirtuin 6 homolog (LvSIRT6) is involved in immune response by modulating hemocytes ROS production and apoptosis
Fish & Shellfish Immunology
Author(s): Junjie Nie, Zhixue Yu, Defu Yao, Fan Wang, Chunhua Zhu, Kaihui Sun, Jude Juventus Aweya, Yueling ZhangAbstractThe histone deacetylase, sirtuin 6 (SIRT6), plays an essential role in the regulation of oxidative stress, mitochondrial function and inflammation in mammals. However, the specific role of SIRT6 in invertebrate immunity has not been reported. Here, we characterized for the...
Phytochemical-loaded electrospun nanofibers as novel active edible films: Characterization and antibacterial efficiency in cheese slices.
Author(s): Gülden Göksen, Maria José Fabra, H. Ibrahim Ekiz, Amparo López-RubioAbstractThe aim of this work was to develop novel active edible electrospun coatings with antimicrobial properties through the encapsulation of essential oils (EOs) from two widely used spices (Laurus nobilis and Rosmarinus officinalis) for inactivating or reducing spoilage and pathogenic microorganisms. Firstly, EOs...
Prevalence and Genetic characteristics of Cronobacter spp. from Food and Human Clinical Stool Samples in Wenzhou, China 2008-2018
Author(s): Yi Li, Leyi Zhang, Yuqin Hu, Chengji Hong, Airong Xie, Yuejin Wu, Zhihui, Shangguan, Lingling Mei, Biao Zhou, Yanjun Zhang, Lei FangAbstractPathogenic Cronobacter species are responsible for life-threatening illness in neonates. A ten-year comprehensive survey was conducted to examine the population structure and antimicrobial resistant patterns of Cronobacter isolates from food (n=78...
Protection against fowl cholera in ducks immunized with a combination vaccine containing live attenuated duck enteritis virus and recombinant outer membrane protein H of Pasteurella multocida
Avian Pathology
Culture‐independent tracking of Vibrio cholerae lineages reveals complex spatiotemporal dynamics in a natural population
Populations of the bacterium Vibrio cholerae consist of dozens of distinct lineages, with primarily (but not exclusively) members of the pandemic generating (PG) lineage capable of causing the diarrheal disease cholera. Assessing composition and temporal dynamics of such populations requires extensive isolation efforts and thus only rarely covers large geographic areas or timeframes exhaustively...
In situ Treatment With Novel Microbiocide Inhibits Methicillin Resistant Staphylococcus aureus in a Murine Wound Infection Model
Increased prevalence of antibiotic resistance in skin and soft tissue infections is a concerning public health challenge currently facing medical science. A combinatory, broad spectrum biocidal antiseptic has been developed ("ASP") as a topically applied solution to potential resistant and polymicrobial infected wounds that may be encountered in this context. The ASP-105 designate was evaluated...


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Cyrus Starring in Annie Get Your Gun
Billy Ray Cyrus will star as Frank Butler in a staged concert production of Irving Berlin's Annie Get Your Gun in Toronto. He replaces Canadian country singer Paul Brandt, who dropped out for personal reasons. The show will preview on Aug. 1 and run through Aug. 20 at Massey Hall. Cyrus' acting credits include the TV series Doc and the David Lynch film Mulholland Drive.
Tags: Billy Ray CyrusPaul Brandt

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redpajama_set_name: RedPajamaGithub


text:
Rainbow Six Siege free for Xbox Live Gold members this weekend
Rival superhero teams for Captain America: Civil War revealed
What I want to see from Sony at E3 2015
Final Fantasy XV Review
An hour of Gears of War: Ultimate Edition showcased
Over an hour long footage of the upcoming Gears of War Ultimate Edition was posted up on MKIceAndFire's youtube channel. The Gears of War Ultimate Edition is a remastering of the first game alongside various new additions. This version of the game will also retain some of the original features of the originally released 2006 title including original score and slightly tweaked voices.
For the majority of things, the game has been touched up in all the right places. This includes art assets, motion capture and animations, control schemes and general textures. As the video title suggests, you can find the entire walkthrough of the game on the youtube channel as it gets posted up.
Having not actually played any of the Gears of War games, I can definitely appreciate the polished remaster job that the Ultimate Edition has provided for the game. It's definitely a great looking game and I wouldn't be opposed to giving it a go. Gears of War Ultimate Edition will be out on the Xbox One on August 25th. A Windows 10 version was also announced during E3; however there is no solid release date yet.
Gears of War: Ultimate EditionThe CoalitionThird person shooter
G'day! I'm Patrick Nguyen and have been having an affair with video gaming since the ripe old age of 4. Primarily a PC gamer, I dabble with many Nintendo titles and indie games. My utter passion is the continued growth of the gaming scene in Australia! You can reach out to me on my twitter @NguFootman


It's difficult to believe that 2018 is nearing its end, but here I sit with the end of the year staring me in the face. It's been a busy year sitting here at the keyboard. Since taking a little bit of a break writing, I've nearly worn out the seat of one pair of pants while pecking away at the keyboard. I noticed my "new" keyboard now had letters nearly rubbed off. Apparently, I use the letters "a" and "n" the most. Good thing I happen to know where they're at.
At any rate, I've mostly been involved with the series, Lifting the Veil, with the goal of completing it by the end of the year. It looks like I'll be able to complete that goal. What initially began as a two-book series quickly blossomed into four. I recently received the third book, which is now titled Lifting the Veil: Emergence, from the first editing phase. So, this next week will be spent going through those. Ugh. Have I ever mentioned that I hate editing? No, well, I do. I think I'd rather shove push pins under my fingernails. But, without it, the stories wouldn't be what they are. So, yay for this week.
The cover for Lifting the Veil: Emergence has been completed, with the exception of small details. Oh man is it a great one. Dean Samed has entered new endeavors, so I had to seek out another cover artist. Based on his recommendation, I selected J Caleb of J Caleb Design. I can't wait to show off the cover he designed. Once I finish this first round and send the manuscript off for the second round, it will take about a month to finish that process. When it's about a week from getting it back, I'll do a cover reveal.
While waiting for the edits, I managed to get about one-third of the way through the rough draft of the fourth book, Lifting the Veil: Risen. And as Emergence is in the next editing process, I'll continue along with that. So, hopefully Risen won't be long following. I have to say that it's been an interesting series to write, as the forehead marks on my desk will attest to. At times, it has been the most frustrating book to pen, and at others, the most fun. I will both miss it and Snoopy dance with joy when the series is completed.
Once this series has reached its conclusion, I'll start back on Red Team. I'll have to read through it to get my mind in synch with the team, but I have so many notes and ideas that need put into coherent sentences. I've long thought about heading back to A New World, but I have a couple of new ideas about that one that are in a battle with one another. So, I'll let those thoughts battle with each other for a while longer. Once one settles in, I'll go back to wrap up the threads in that story.
As a last note, Lifting the Veil: Winter has been released as an audio book. Mark Gagliardi has done another wonderful job and I'm so pleased that I found him so long ago as a narrator.
As always, I really appreciate the support you've given me over the years. It really humbles me to think about where this has gone since I put the first word down on A New World. So, thank you!


I recently upgraded our network environment to McAfeee DLP 9.4.2 and successfully migrated my policies from the 9.3 version. I have created a rule to make all CD/DVD Drives read only and it is working just fine. I also have a rule to Block All USB access, with a few limited exceptions. I would like for one of those exceptions to be any local user, so that our support team will have access to the USB ports on any machine with the restricted local administrator account. Each time I add this exception I get the below error message and I am unable to apply the policy until I remove this exception. Can anyone explain why I am getting this error and if there is a way to bypass it?
That is referring to your backwards compatibility settings for your policy. You can find the setting in Menu > Server settings > Data Loss Prevention.
Thanks for the answer, that resolved the issue I was having. I was looking for where that setting had been moved to, well now I know. Thanks again!


04/05/2017 … Oh no! Not another arena-filling comedian with a quasi-academic tome about neuroscience.
04/01/2017 … Trevor Noah's rise through the international comedy circuit to become host of The Daily Show has been swift.
19/12/2016 … With A Horse Walks Into A Bar, Israeli writer David Grossman accomplishes the seemingly impossible and transposes an entire stand-up show into a novel.
08/11/2016 … Bill Bailey isn't, as it happens, the first bearded comedian to have written a bird book.
21/10/2016 … Twenty-five years years after making his debut on Radio 4's On The Hour, Alan Partridge is the comedy gift that keeps on giving.


text:
The It List: Celebrate the Fourth of July holiday with 'Stranger Things' Season 3, 'Spider-Man: Far From Home' and the best in pop culture the week of July 1, 2019
Now is the perfect time for a "Summer of George"... and, you know, Jerry, Elaine and Kramer! That's because the very first episode of Jerry Seinfeld's sitcom premiered on July 5, 1999, 30 years ago this week. The then-named Seinfeld Chronicles debuted with no Elaine and no Kramer (Michael Richards's character
"Choose Your Own Adventure."
Seinfeld at 30: How the 'Show About Nothing' Made Such a Major Impact
(Photo by NBC) It's been three decades since Seinfeld first premiered on July 5, 1989, changing the sitcom narrative forever. From the show's core characters of Jerry...
Rotten Tomatoes 2019-07-05
Seinfeld is 30 years old. Here are 5 ways it changed television.
After the "show about nothing," TV sitcoms would never be the same. Seinfeld , one of the most successful TV shows ever made, turns 30 on July 5, 2019. Its pilot, "The...
Vox 2019-07-03
30 years after debut, 'Seinfeld' remains the master of its domain
WTOP's Jason Fraley salutes 20 years of 'Seinfeld' Jason Fraley https://wtop.com/wp-content/uploads/2018/05/SEINFELD-jfray-2.mp3 Download audio See our WTOP staff picks of...
Wtop 2019-07-05
'Seinfeld Experience' inspired by iconic sitcom to launch in NYC
Coming soon: a show about nothing, that could really be something. "The Seinfeld Experience," opening in New York's Gramercy neighborhood this fall, promises an immersive,...
New York Post 2019-06-27
\'Seinfeld\' actors recall \'laugh machine\'
It's been 30 years since "Seinfeld" catapulted Jerry Seinfeld, Julia Louis-Dreyfus, Jason Alexander and Michael Richards into superstardom. But the sitcom, which premiered (as "The Seinfeld Chronicles") July 5, 1989, featured other familiar faces, too. They just weren't familiar at the time. Before starring in hit TV series from "Lost" to "Breaking Bad," many now-established...
Hulu to Add "Yada Yada Yada" Button to Shuffle \'Seinfeld\' Episodes
The button will appear in the Hulu app in celebration of the sitcom's 30th anniversary. Hulu is offering a new way to watch old episodes of Seinfeld. Starting July 5, viewers using the Hulu app on Apple TV set-top boxes will be able to watch randomized episodes of the sitcom using the "Yada Yada Yada (Shuffle)" button. The launch of the shuffle feature coincides with the 30th...
'Stranger Things' Season 3 goodies are here just in time
With Stranger Things season 3 on the way, it only makes sense that the show's impressive merch train would fire up for a new run. And we're not just talking about the officially-licensed Stranger Things toys and games either. At this point, fans have had about two years to boast about their love for season two (and the show in general) while bringing their original Stranger...
'Seinfeld' at 30: From Patton Oswalt to Kathy Griffin, guest stars recall a comedy machine
It's been 30 years since "Seinfeld" catapulted Jerry Seinfeld, Julia Louis-Dreyfus, Jason Alexander and Michael Richards into superstardom. But the sitcom, which premiered (as "The Seinfeld Chronicles") July 5, 1989, featured other familiar faces too. They just weren't familiar at the time. Before starring in hit TV series from "Lost" to "Breaking Bad," many now-established...
The Mercury
\'Seinfeld\' at 30: From Patton Oswalt to Kathy Griffin, guest stars recall a comedy machine
It's been 30 years since "Seinfeld" catapulted Jerry Seinfeld, Julia Louis-Dreyfus, Jason Alexander and Michael Richards into superstardom. But the sitcom, which premiered (as "The Seinfeld Chronicles") July 5, 1989, featured other familiar faces too. They just weren't familiar at the time. Before starring in hit TV series from "Lost" to "Breaking Bad," many now-established...
Finger Lakes Times


f you are thinking of selling your South Dakota ranch farmland, or have an interest in finding one... consider working with one of our South Dakota real estate land brokers capable of all phases surrounding farms and ranches for sale in South Dakota. Combined, our agents offer more than 133 years experience in ranch real estate sales. We have the right marketing affiliations for our selling clients, an unsurpassed knowledge of the land characteristics and a proven track record representing buyers interests throughout South Dakota and the western United States.
The LeBar Ranch of "South Dakota" consists of 6,280.86 acres of grazing lands and is located near Provo, South Dakota approximately 10 miles south of Edgemont, SD. Edgemont is located in the very southwestern corner of the state. The area is primarily comprised of ranch operations of various sizes. In addition to the Lebar Ranch of South Dakota. The Lebar Ranch of "Nebraska" (2,972 acres) is also available.


While global milk supplies have increased significantly, Lakeland Dairies' CEO Michael Hanley believes that dairying will continue to outperform other sectors for the foreseeable future and he remains optimistic.
"Lakeland Dairies has an intensive focus on new business development with high quality contracts and a growing customer base. Our dairy plants have world class technology, maximising the value of every litre of milk sent to us for processing. We have made major investments in facilities and we have the finance to invest further where needed.
He said Lakeland customers want more of our dairy products and we have the capacity to meet that growing demand with our expertise in long-life UHT technology, ice-creams, butterfats, powders, caseins, lactose and whey. "We have successfully built, commissioned and paid for a new milk dryer at Bailieboro. This has streamlined our butter and powder production onto one major site. Our global foodservice distribution facilities are also being upgraded. We have the required international distributor partnerships and we have access to all main dairy markets across the world.
Lakeland milk suppliers have indicated that they will increase milk production in the years ahead, taking the co-op's milk processing close to 1 billion litres annually by 2020.
"We're providing information and advice to encourage efficient milk production and to plan for profitable expansion in the future. Our priority is to achieve all future growth on a long term and sustainable basis. We will continue to pay a leading milk price while ploughing all of our growing profits back into the Lakeland, farmer owned and controlled co-operative," he said.


Ambition have been assigned on an Insolvency role with an established and respected firm in their Southampton office who are looking for an Insolvency Manager. For consideration, you would ideally be an experienced Insolvency Manager or an Assistant Manager who is looking to progress into a full Managerial role.
As Insolvency Manager, you and your team would be working on formal caseload of corporate and personal insolvency assignments; however, the firm have been getting involved with advisory projects in recent times.
You need to have experience managing and developing a team within insolvency and strong formal insolvency case experience on both corporate and personal assignments i.e. Administrations, Liquidations, Voluntary Arrangements and Bankruptcies.
You would be based in the Southampton office but there is requirement to travel to other clients every so often.
Insolvency and/or Accounting qualifications are highly desirable but in this role, experience is the key factor.