Quatchi's Sport of the Day: Luge!
Programming note: On tonight's Simpsons, Homer and Marge go to Vancouver as members of the American Olympic curling team. I had faint hopes that Quatchi would have a cameo, but instead they referenced a fictional mascot named Chillbert.
Here's closeup of a Chillbert pin — he's "one of the four winter games mascots." We don't get to see the other three.


text:
Q: Extracting/Selecting text from .srt files in android I want to use the entire text from a .srt file for an android app.
One way of doing this was to:-

*

*open the file in an app downloaded from the play store called "Subtitle Editor" and save  it as a .txt file.

2.Open this .txt file in "Polaris Viewer 4.1".
3.Select text by double-tapping the screen and then adjusting the selectors.
But in this manner, I was allowed to select only a maximum of one page at a time due to a limit on clipboard/Polaris Viewer capacity.
Thus, in order to select the entire text from a .srt file which is atleast 10 pages, I need to repeat this process for each of the 10 pages.
Hence, this is very cumbersome.
Is there any other workaround/solution by which I might be able to do this..??
The solution can be both programmatical or use a software to do so...
Thanks.

redpajama_set_name: RedPajamaStackExchange


Lily Collins has always had the air of someone from another era. Maybe it's those signature thick, Victorian-esque arches, the uncanny resemblance to Audrey Hepburn, or the actress's undeniable poise on screen and off—probably all the above. Either way, it seems rather fitting that Collins's latest role lands her in the Hollywood of a bygone era. Amazon's newest series, The Last Tycoon, takes place in 1930s Los Angeles and stars the recent redhead as a go-getter named Celia with behind-the-camera aspirations.
"She knows that she wants to be in this industry behind the scenes and work her way up and become a mogul of sorts of her own," Collins tells us. "But she's a female, and women in the 1930s normally were not known to do that. I think she was so ahead of her time."
She might play someone on the cutting edge of her own era, but Collins says the role called for a bit of a crash course in retro beauty. Ahead, she tells us her thoughts on dark lipstick, the perfect cat eye, and the surprising story behind her bold new hair color. But first, check out a two-part video tutorial on the Jazz Age–inspired look seen here.
BYRDIE: What did you learn about the beauty of that era while sitting in the makeup chair for this project?
Lily Collins: The shape of the lip was much rounder, so the way to apply lipstick was different, going above the edges and smoothing out different areas. The way blush went on was very different. It wasn't about contouring; it was about flushed faces. And I love the black eyeliner with the flick—it's so beautiful. I love a good cat eye, and that can really go with any generation. And the hair itself! It's that hours and hours of prep to have it actually last a couple of days. The amount of spray and hot tools they used back then kept in the heat so much more.
Maybe they were damaging, but you can have that look for days on end afterward!
BYRDIE: Your current hair color is for another project you're working on. Are you going to keep it after filming wraps?
LC: It's funny—in the beginning, I was apprehensive and didn't want to do it, because I had other commitments, and then I just dove in. The woman who did the color did an incredible job because it changes color in the light. It goes purple to watermelon to pink to red. So I would love to keep it as long as I can because I think it's really fun. And the more you wash it, the brighter it gets, so it starts changing color even more.
BYRDIE: You wear a dark lip so well! Do you have a favorite shade right now?
LC: Thank you! Well, now with my hair I haven't experimented at all, but I love the color I have on today. I used to love dark orchid colors. I'm really pale-skinned, so I guess dark really works. It's funny—I used to mute my own color because it's so bright, and now I've gone the exact opposite. I like the contrast of the skin tone and having it be bold.
BYRDIE: What's your best tip for pulling off the look?
LC: Eat really carefully. Use a straw. Believe me—when I'm putting makeup on and I do a dark lip myself, I'm the first to admit how long it takes me. It's a process, but you feel accomplished when it's done. I guess just being more aware that you have it on.
BYRDIE: You were just filming in Korea. Did you pick up any amazing beauty tips/tricks?
LC: Well, I'm a big face mask person—I love Lancôme's face masks—and it was just so interesting to walk around and see all the different ones they have in Korea. They have animal-shaped ones, superhero ones, and the names are hilarious. Skincare is such a huge part of their daily life there. They have eight or nine processes of putting on different products, so I was just fascinated with walking around and seeing all of them. I was filming, so I was a little nervous to try anything new, but the fashion there is so cool too.
Same with Tokyo—anything goes. I love the little schoolgirl look. They do it so well. Nothing is odd there. I mean, self-expression in fashion and beauty is whatever you want it to be, and I appreciate that, and they have such an acceptance of differences. You'll see a lot of barely there makeup and a bold red lip, and that's it. It is quite striking. It was interesting to immerse myself in that world.
Get the tutorial for Collins's retro beauty look below.
Head over to Who What Wear to see Collins's thoughts on her ever-evolving style, the career she'd be pursuing if it weren't for acting, and more.


Printersdriverr.com – We Provide Download Links Its easy to find the right driver to install HP 900 Inkjet Printer series Driver. We have the most supported Printer Driver Hp Product being available for Free Download. And for the most popular products and devices Hp.
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See the conference agenda and educational content. MINDBODY: Connecting the World to Wellness Staff Log In. | See more ideas about Health wellness, Yoga meditation Massage. Home; MindBody Online.
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Luni 19 noiembrie 9: 32 Life | Smart Woman. Get to know the keynote speakers at this year' s BOLD conference, including Billie Jean King.
Explore Michelle Kellogg' s board " MindBody" on Pinterest. Summit Action has 4 current team members, including Founding Partner Brock Saunders. About the Summit This online video summit is a rare opportunity for you to get direct access to some of the leading medical doctors health advocates weight loss revolutionaries on the planet.
Want to keep up to date with our latest events, programs, news, and information? Fill out the form below to sign up for the Summit Area YMCA newsletter sent directly to your e- mail! We would like to show you a description here but the site won' t allow us.
MINDBODY provides business management software to yoga and Pilates studios, fitness facilities, spas, salons and other retail clients, giving them the ability to conduct ecommerce and their customers the ability to book and pay for appointments and classes ( e. , haircuts, personal training) directly over the e how MINDBODY business management software serves gyms, spas and salons worldwide, and helps people find and book with them.


Chris Reed is looking for information on this piece of equipment, found near a road by Nome Creek in Alaska.
I recently found these near the road leading to Prindle Mountain Campground near Nome Creek (up Steese Highway) in Alaska. I am curious as to what it looked like together, what it was used for and, most of all, how they got it way back in the hills so many years ago. Wasn't sure if it was part of the bucket dredge that ran some time ago or a different operation since it was quite a ways from the tailings left by the dredge. I didn't notice until I got home and reviewed the pictures that there's a plate on the boiler face. Maybe next year I'll be able to get a closer photo.


There are few places in the USA you can live where you can make better use of solar energy products than St. Augustine, Florida. It's full of abundant sunshine almost all year long. There is also no one better to install your solar energy products than TEO Solar, also known as The Energy Outlet! We have been St. Augustine's premier solar services provider for over 30 years now.
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IGT software developer is one of the giants of the gambling industry founded in Las Vegas, Nevada. The company manufactures casino games and slot machines for many land-based casinos before expanding into the online gambling industry recently. The company during its existence on the stage was awarded many prestigious awards like the U.S. Senate Productivity Award back in 1989. A year later the company released their the very first themed slot machine Red White&Blue. AT that point with the success of this game, IGT software developer decided to brave some new waters as they ventured into the massive European gambling market which was a good call. Since then, the company became one of the strongest contenders in the European gambling market offering their services and products to the majority of European land-based casinos. Back in 2010 IGT was awarded the Best Slot Manufacturer among many other awards. As the success of the company was on the rise, IGT decided to expand into the online gambling market. They decided to push even further. Back in 2010, the company bought WagerWorks, launched IGT interactive, and started their venture to the online gambling market with a huge success at the very beginning.
Play Noah's Ark by IGT and enjoy generous cash rewards.
IGT software developer offers both online and mobile games while delivering the most compelling online gambling experience. The company is greatly dedicated to providing the best experience to worldwide players offering the best video slot titles you can play only at IGT online casinos.
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The highest IGT progressive jackpots were won on a popular MegaJackpots title. A lucky player headed home with a staggering win of $2,148,947 back in September 2013. The second biggest jackpot was won back in January 2014. A lucky player won $1,777,233.
IGT software developer is one of the leading companies in the industry is present in the online gambling market since 2010. The software is fantastic offering exciting games like IGT video slots, IGT progressive jackpots and of course, plenty of table games. IGT games boast a high quality which for other software developers can match. Players enjoy playing their favorite games at IGT online casinos as the games offer the experience almost identical to that one you can see at any land-based casino. In terms of IGT online casinos quality, it is highly unlikely to find an online casino venue operating on IGT which is bad.
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text:
Carl Franz Viktor Gielen (* 9. April 1900 in Münster; † 24. September 1987 in Köln) war ein deutscher römisch-katholischer Priester und Kölner Dompropst.

Leben
Gielens Vater war der spätere Oberbürgermeister von Neuss und München-Gladbach Franz Gielen. Nach dem Abitur am Quirinus-Gymnasium in Neuss und Militärdienst studierte Gielen in Bonn Katholische Theologie und wurde aktives Mitglied der katholischen Studentenverbindung Arminia Bonn im Kartellverband, dem er sich bis zum Lebensende herzlich verbunden fühlte. Nach der Priesterweihe 1923 durch Kardinal Karl Joseph Schulte war er in der Gemeinde St. Antonius in Barmen tätig. Ab 1925 studierte er Kirchenrecht an der Päpstlichen Universität Gregoriana in Rom und war gleichzeitig Vizerektor des Kollegs Maria dell'Anima. 1927 schloss er sein Studium mit der Promotion zum Dr. iuris canonici ab.

1928 wurde er Domvikar in Köln, 1948 Offizial des Erzbistums Köln und Leiter des Erzbischöflichen Gerichts, 1950 Domkapitular und 1960 Propst des Kölner Domkapitels. 1978 verzichtete er auf sein Kanonikat.

Wirken
Carl Gielen war über Jahrzehnte hinweg eine führende Person im Kölner Klerus und gehörte als Mitglied, Vorsitzender oder Geistlicher Beirat insgesamt 32 Gremien und Organisationen an. Besonders unterstützte er Geschichtsvereine und Fördervereine für Museen. Von 1968 bis 1978 war er als Dompropst Hausherr des Kölner Doms, dessen Erhaltung und Restaurierung für ihn ein ganz besonderes Anliegen war.

Sein Nachlass befindet sich im Historischen Archiv der Stadt Köln.

Ehrungen
Gielen war seit 1947 Päpstlicher Geheimkämmerer, Papst Paul VI. ernannte ihn zum Apostolischen Protonotar. 1978 wurde er vom belgischen König Baudouin zum Commandeur de l'Ordre de Léopold ernannt. Carl Gielen wurde auch Ehrenphilister der katholischen Studentenverbindung Rheinpfalz-Köln im KV.

Literatur
 Deutsches Biographisches Archiv, II 447,75 und III 295,1-4
 Robert Jauch in Siegfried Koß, Wolfgang Löhr (Hrsg.): Biographisches Lexikon des KV. 1. Teil (= Revocatio historiae. Band 2). SH-Verlag, Schernfeld 1991, ISBN 3-923621-55-8, S. 38 ff.
 Robert Steimel: Kölner Köpfe. Steimel, Köln 1958.

Weblinks

Offizial (Erzbistum Köln)
Dompropst (Köln)
Päpstlicher Geheimkämmerer
Träger des Leopoldsordens (Kommandeur)
Person (Neuss)
Deutscher
Korporierter im KV
Geboren 1900
Gestorben 1987
Mann

redpajama_set_name: RedPajamaWikipedia


text:
Are boys are being feminized by the public schools?
Early Morning Thoughts, March 16, 2019
I received an e-mail from Gary North this morning, and his comments/questions raised an interesting subject I hadn't given much prior thought to, but also have realized, in the back of my mind, is an important topic for discussion. Gary's comments:
"Today, boys are being feminized by the public schools. This is a systematic program.
"If you [were] asked by a young father of a boy what you would recommend that he do for his son [to] toughen him up mentally, what would you tell him? Homeschool him? Have him join the Boy Scouts? Have him get an after-school job? Have him donate time alongside him at his service club or church? Give reasons for your recommendation."
https://www.garynorth.com/public/19278.cfm
After reading Gary's online post, I more or less avoided his initial premise "When the newly created public school system began hiring unmarried young women in the 1860's to teach in small town schools and rural schools, the connection between fathers and sons was severed in a fundamental way. These women worked cheap. Married men could not compete. Women became the teachers of America's boys."
My first reaction to Gary's e-mail was focused on the 'This is a systematic program' part of his e-mail comments, and I am not convinced that having women teachers was the problem. The teachers I remember from my own youth were a mixture of men and women. I have no problem with women teachers. If the object is to learn, and if a child is raised in a cohesive family structure, he/she would and will get training from both father and mother. That's a balance that is necessary. In my opinion.
But, let's remember that the United States started out as English colonies, and 8 of the first nine colonial colleges were religious universities, not secular. And it was largely colonists from that English and largely Protestant college training that started the country's governments.
"Due in part to a collegiate curriculum that drew from the advanced writings of Scottish and Enlightenment thinkers in political economy, the colonial college alumni designed a system of government destined to serve as a model for the world."
https://education.stateuniversity.com/pages/2044/Higher-Education-in-United-States.html
The Articles of Confederation and Perpetual Union was the agreement among the 13 original states of the United States of America that served as its first constitution. It was approved, after much debate, by the Second Continental Congress on November 15, 1777, and came into force on March 1, 1781, after being ratified by all 13 states. The Articles included one up-front section regarding state sovereignty:
"Article II. Each State retains its sovereignty, freedom, and independence, and every power, jurisdiction, and right, which is not by this confederation, expressly delegated to the United States, in Congress assembled."
During this process, John Adams noted that, "I expressly say that Congress is not a representative body but a diplomatic body, a collection of ambassadors from thirteen of sovereign States…."
After 8+ years of war for independence (April 19, 1775 – September 3, 1783), the 13 original English colonies finally became 13 independent sovereign states (i.e. countries) via the Treaty of Paris (signed on September 3, 1783). England finally recognized the legality of our American state's sovereignty.
"His Brittanic Majesty acknowledges the said United States, viz., New Hampshire, Massachusetts Bay, Rhode Island and Providence Plantations, Connecticut, New York, New Jersey, Pennsylvania, Delaware, Maryland, Virginia, North Carolina, South Carolina, and Georgia, to be free sovereign and independent states, that he treats with them as such, and for himself, his heirs, and successors, relinquishes all claims to the government, propriety, and territorial rights of the same and every part thereof."
https://highlanderjuan.com/wp-content/uploads/2019/01/John-Sutherland-State-Sovereignty-8-17-10.pdf
Inasmuch as the Articles of Confederation helped define the original central government system for the new 13 American states, that confederation was, according to some at the time, far too weak as a central government (it only provided for a congress of the state's representatives). Some 18th century thinkers thought we needed a much stronger central government, one that included an executive branch and a judicial branch. Thus, on June 21, 1788 a new 'Constitution for the united States' was ratified, to become effective on March 4, 1789. Was this a good evolution of government? Maybe, maybe not. This is what Bill Buppert thinks:
"The Constitution is an enabling document for big government. Much like the Wizard of Oz, the man behind the curtain is a fraud. In this case, for all the sanctimonious handwringing and the obsequious idolatry of the parchment, it sealed the fate of our liberties and freedoms and has operated for more than 200 years as a cover for massive expansion of the tools and infrastructure of statist expansion and oppression."
And so, with the Constitution in place, the country's central government started its journey into what is a massive government today. In the country's early days, the education system started to change and grow also.
"Educational opportunities for young women followed a comparable pattern. Families often wondered how a young single woman could be self-supporting or contribute to the family welfare. A growing national demand for trained teachers due to the "common-school movement" of the 1830s provided one answer. Women could achieve financial independence and respectability within a rather rigid social structure by attending a normal school or female seminary that provided them with an education for employment as teachers in the ever-expanding nation."
So, now we get to the nub of Gary's problem – the 'common-school movement.' Although the United States had originally been 'the shining city on the hill,' we were viewed that way because we supported and defended the individual, not the group, not a religion, and not a government. When the group started to overwhelm importance over that of the individual, we started our decline as a people. One size does not fit all. Individual strength and independence is still of greatest importance to all humans. In my opinion.
My theory of what causes the American males to seem less masculine is directly related to government policies, not to whether teachers are male or female. It is the central government, Comrade, that chooses to force the states into a single system of actions and controls. Is the Constitution the problem as Bill Buppert suggests, or is it those who control the government who are at fault? If our elections are not valid, and if there is a secret, shadow government that controls this country's central government and thus our laws, our economy, our media, then shouldn't we be looking at that problem?
It is my perception and opinion that our country, its system of laws, its economy, and its culture, have been, and are still being, systematically attacked and destroyed, by an evil set of shadow rulers – people who have no allegiance to America, and who have historically described themselves as 'the great destroyers.'
Looking back, I liked the Articles of Confederation if for no other reason than under that voluntary agreement we had no strong central government and the sovereign states could act on their own. So what if the states did not always agree on any given subject? Isn't that what separation of powers is all about? Isn't having differences in the states how we learn from each other? When we have one central ruling authority, one that deals exclusively in fraud, force, and violence in order to assure compliance, aren't we all living under one set of rules as slaves to a plantation master?
What, exactly, is wrong with letting cities and towns establish their own sets of voluntary rules for their own citizens? This is one huge country, and we have many subcultures in our land – why not let the people rule themselves? Who, besides the shadow rulers, needs a strong central government?
Like Buppert, I am no lover of government controls over me – I want no organized force and violence exerted on me and my family – we have enough troubles just surviving and thriving in America. I want all human relationships to be voluntary, and I don't need government to force its policies on me. I'm an adult and wish to be treated as an adult. I'll take care of my own – just leave me alone.
Let there be differences in how we teach our children. If a locality wants to feminize its males, let them do it. If a locality wants strong masculine males, let them do it. Then let the states compete for which education process is better. That's how we all learn.
By Highlander|2019-06-13T19:34:30-06:00March 16th, 2019|Early Morning Thoughts|0 Comments

redpajama_set_name: RedPajamaCommonCrawl


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Posted in Where Should You LiveTagged adaptation period, moving guide, moving tips and tricksLeave a Comment on Living In The Suburb vs City: How to Choose?
Are you tired of being single? And are you thinking of making a big step to change your relationship status? If you're living in a big city, the first step you could do is to move to a smaller town as researches show that it's much easier to find a partner in a smaller place. Still, if you like big places and are not quite sure where to go, here is our list of 5 best cities for singles in Canada.


I just replaced the toner cartridge and the LCD continues to display the message "Toner Life End" or "Replace Toner".
I still receive the error message "Replace Parts Drum" or "Drum Stop" after I replace the drum unit with a new one.
Unable to print or scan when using Brother iPrint&Scan from my Android™ mobile device.


text:
Spanish New Orleans and the Caribbean / La Nueva Orleans Y La Caribe Españoles (Hardcover) By Alfred E. Lemmon (Editor) $44.95
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Lady M: The Life and Loves of Elizabeth Lamb, Viscountess Melbourne 1751-1818 (Hardcover) By Colin Brown $27.95
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From hobby farms to commercial agriculture exposures such as feed and seed dealers, it is important to identify risks associated with whatever it is you produce. Parrott Insurance knows farming and commercial farm exposures unique to the state of Iowa. We proudly represent top-rated insurance carriers that are specific to agribusiness to ensure you get the coverage you need at a price you can afford.
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Irish soda bread is a very easy type of bread that is made with baking soda as the leavening agent instead of yeast. The traditional bread consists of only flour, salt, baking soda, and buttermilk. This modern version also includes butter, which is cut into the flour mixture, to add more tenderness and richness to the bread.
Buttermilk is a key ingredient in soda bread due to its acidic properties. The lactic acid present in buttermilk reacts with the alkaline properties of the baking soda to create carbon dioxide, rising the bread.
Irish soda bread first came to be in the early 1800s when baking soda first became available in Ireland. Most flour available in Ireland at the time was made from soft wheat (meaning it had a low protein content). Soft wheat does not work well to make yeast breads, but it works beautifully for chemically leavened bread.
Soda bread gained huge popularity during the potato famine, which started in 1845. Due to the lack of potatoes, which were a major food source in Ireland, soda bread became an easy, inexpensive, and filling staple on the Irish table.
Everyday Irish soda bread, like this one, was very simple without any add-ins. Versions of soda bread that include dried fruit, nuts, or other additional ingredients were only made for celebrations. However, this base recipe can easily be the template for endless soda bread variations.
Because soda bread is leavened with baking soda, you want to minimize the gluten formation in the bread. Stir the ingredients together just until the flour is moistened, but do not knead the bread the way you would a yeast bread.
A dutch oven is truly the best vessel to bake this bread in. If you do not own a dutch oven, the bread can be baked on a sheet pan or in a skillet, however it won't achieve quite the rise that it gets when baked with a lid on.
The texture of this bread is a bit like a scone. It is best eaten the same day it is made, however, leftovers can be toasted to refresh them for a few days after.
This recipe can be used as a base recipe to create different flavor variations. Sugar and currants could be added to create a traditional tea cake style soda bread that is served at celebrations. You could also add fresh herbs or cheese to make a savory variation.
Flour is the main structure of the bread. There are many variations of soda bread, some made with whole wheat flour, some made with white flour, or a combination of the two.
Baking Soda is the leavening agent for this bread.
Butter adds some richness and helps keep the bread tender.
Buttermilk is the moisture for this bread and its acidic properties activate the baking soda to make the bread rise.
This is a very basic Irish soda bread made with only 5 ingredients. Irish soda bread is an easy quick bread that is made without yeast and is leavened with baking soda.
Preheat the oven to 450 F (232 C). Line a 4 or 5 quart dutch oven with parchment paper. If you do not own a dutch oven, you can bake this on a sheet pan or in a skillet, but it will not rise quite as much as it will if you use an enclosed environment.
In a large mixing bowl, whisk together the flour, salt, and baking soda until thoroughly combined. Add the pieces of cold butter into the bowl and use a pastry blender or a fork to cut the butter through the flour mixture.
Add the buttermilk in slowly and use a rubber spatula to stir until a soft dough forms. You want the mixture to be a very soft dough that is almost on the verge of becoming a thick batter. Different varieties of flour are more absorbent than others, so this is why a range of liquid is listed.
Once you have the desired consistency, transfer the dough into the prepared dutch oven and use the spatula to roughly shape it into a disk. Use a sharp knife, or a bench scraper, to score an X into the dough. You want to cut very deep, almost all the way to the bottom of the dough.
Put the lid on the dutch oven and put it in the oven. Bake at 450 F (232 C) for 35 minutes. Remove the lid of the dutch oven and bake for another 10-12 minutes until the top is a deep golden brown.
Serve with butter and salt. This bread is best eaten the day of.
Can't wait to make this when we celebrate St Patrick's Day (early, this Friday!). Love your No Knead Skillet Bread. My daughter and I make that recipe all the time!
Oh let me know how it goes! It is definitely a little different than the no-knead bread. More scone like. But so delicious!
So great to hear! Thanks so much, Candace!
Hello! Ive tried other sour bread recipes before, after being able to eat it while traveling around Ireland. Yours was the only one to replicate that amazing flavor! I used 2 cups of wholewheat to 1 of plain flour and followed the recipe as written and it turned out great! I'm so happy I can finally have a piece of Irish sour bread whenever I want. Thank you!!
Wow Bruna! What a compliment! Thank you so much for your feedback. I'm so glad you enjoyed it!
question: my dutch oven is 7 quart. what kind of allowance should i make? thanks – can't wait to make this!
Hi Mary! The width difference of a 7 qt dutch oven isn't enough that it should make that much of a difference! You should be okay to bake it as recommended.
that's just the answer i was hoping for!!!!! thanks so much for responding.
I just put it in the oven about 5 minutes ago. Is the dough supposed to be on the sticky side? I didn't want to add more flour.
Hi Maggie! Yes, it will be sticky!
Did you preheat the dutch oven while the oven was heating? Or just place the dough into an unheated pan?
Hi Denise! You do not need to preheat the dutch oven!
This was the worst thing I have ever baked. Followed exact directions. No taste and after even baking longer than called for it still seemed unbaked.
Hi Marlene! I'm so sorry to hear that you had issues with this bread! Since this is a tested recipe I'd love to help you troubleshoot. It sounds like possibly your oven is not heating up to temp. Do you by chance have an oven thermometer to check if your oven is actually reaching the set temperature? This is a common issue with ovens.


text:
Asabee Resigns As SIGA Boss To Join NPP Chairmanship Race
Stephen Asamoah-Boateng is contesting NPP Chairmanship race NPP to open nominations for National Executive Primaries
Stephen Asamoah Boateng was the Director-General of the State Interest and Governance Authority (SIGA)
The Director-General of the State Interests and Governance Authority (SIGA), Stephen Asamoah- Boateng, has resigned as Director-General of the Authority to enable him to contest in the upcoming New Patriotic Party (NPP) National Executive primaries.
This was announced at the closing session of a three-day retreat organised by SIGA, for Chief Executive Officers (CEOs), their Deputies and Board Chairpersons at Kwahu-Nkwatia in the Eastern Region.
Speaking at the event, Asamoah-Boateng applauded the CEOs for supporting him to bring the Authority this far.
According to GBCOnline report, he encouraged them to continuously work in unity to help government achieve its agenda.
The Programme dubbed 'The strategic role of Heads of Institutions in transforming specified entities towards the achievement of Ghana Beyond Aid Agenda', was attended by more than 300 CEOs, their Deputies and Board Chairpersons across the country.
The Director-General of the State Interest and Governance Authority (SIGA), Stephen Asamoah Boateng announced his interest in running for the position of national chairman of the New Patriotic Party (NPP) in September 2021.
Known to many as "Asabee", Mr. Asamoah Boateng, in an interview with Joy News'PM Express also stated that his decision to run for the position is in the best interest of the party and the country.
"Yes, I intend to contest. Whether it is by my performance and the performance of SIGA, let's wait until the time comes, you'll hear about why I'm in there. It is important to Ghana and it is important to the NPP," he said.
Source: ghanaweb.com
Bank of Ghana raises policy rate to 14.5%
Digital transformation drive shared responsibility – Vice-President
'2022 is a year of economic misery' – Ato Forson
AG files suit against Ato Forson for causing financial loss to the state
Digital transformation drive shared responsibility - Vice-President
I'm Proud Of Free SHS; 2020, 2021 WASSCE Results Prove Policy Is Working" – President Akufo-Addo
How to make the best Carrot cake with 4 easy steps
IMF extends debt service relief for 25 low-income countries


text:
Q: Jetty http session is always null (Embedded Container, ServletHolder) I am trying to implement a simple servlet which uses a HTTP session in
an embedded jetty (7.3.0 v20110203) container. To start jetty I use the following code:
Server server = new Server(12043);
ServletContextHandler handler = new
            ServletContextHandler(ServletContextHandler.SESSIONS);
handler.setContextPath("/");
server.setHandler(handler);
ServletHolder holder = new ServletHolder(new BaseServlet());
handler.addServlet(holder, "/*");
server.start();
server.join();

The servlet acquires a session with
HttpSession session = request.getSession(true);

and stores some data in it. Upon the next request it gets the session
with the following code:
HttpSession session = request.getSession(false);

and there the session is always null.
I did not find any information on
the internet about this particular problem. I have also experimented
with setting a SessionManager or SessionIdManager, but that did not seem
to change anything. I suspect I am missing something about SessionManager or SessionIdManager or SessionHandler here, but this is just a wild guess.

A: Your code works fine with this skeletal implementation of BaseServlet:
public class BaseServlet extends HttpServlet {
    protected void doGet(HttpServletRequest req, HttpServletResponse resp) {
        boolean create = "true".equals(req.getParameter("create"));

        HttpSession session = req.getSession(create);
        if (create) {
            session.setAttribute("created", new Date());
        }

        PrintWriter pw = new PrintWriter(resp.getOutputStream());
        pw.println("Create = " + create);
        if (session == null) {
            pw.println("no session");
        } else {
            pw.println("Session = " + session.getId());
            pw.println("Created = " + session.getAttribute("created"));
        }

        pw.flush();
    }

so the session is probably being invalidated somewhere else in your code.
The SessionHandler can also be explicity set using the setSessionHandler() method of ServletContextHandler.

A: //You need to setup SessionManager first. This worked for me.
Server server = new Server(8031);
ServletHandler handler = new ServletHandler();
server.setHandler(handler);

HashSessionIdManager hashSessionIdManager = new
HashSessionIdManager();
SessionHandler sessionHandler = new SessionHandler();
SessionManager sessionManager = new HashSessionManager();
sessionManager.setSessionIdManager(hashSessionIdManager);
sessionHandler.setSessionManager(sessionManager);
sessionHandler.setHandler(handler);
sessionHandler.setServer(server);
server.setSessionIdManager(hashSessionIdManager);
ServletContextHandler context = new ServletContextHandler(ServletContextHandler.SESSIONS);
context.setContextPath("/");
context.addServlet("webservlet.IndexServlet", "/index");
context.addServlet("webservlet.HomeServlet", "/home");
context.addServlet("webservlet.CategoryServlet", "/category");
context.addServlet("webservlet.ProductServlet", "/product");
...

A: The method HttpServletResponse#reset() destroys a newly created session, so a new one is created the next time and so on.


text:
Q: Crystal Reports error "Invalid object format name." for field objects I'm making a dynamic report using Crystal Reports and .NET C#. The content of the report is not fully known until run-time.
The report template only contains Formula Fields and a connection to the database table.
At runtime I collect the wanted data collection, create a new reportDocument object and set the collection to the reports DataSource. Then I collect all field objects from the report template and loop through them, setting the data properties. This works fine as long as the properties is of type string, BUT when it is of type bool or double, it crashes with the following error: "Invalid object format name"
How can I set the data type of the field object to numeric/bool at runtime?
Here's some code:
//Collecting data and setting data source
for (int i = 0; i < ColNumber; i++)
            {
                //Fields

                var DbFieldName = mappingNames[i];
                var fieldType = comparisonObj.GetFieldType(DbFieldName);

                var fieldName = "Field" + (i + 1);
                var ffld = report.DataDefinition.FormulaFields[fieldName];
                if (ffld != null)
                {
                    ffld.Text = "{" + aliasName + "." + DbFieldName + "}";
                }

                var fobj = report.ReportDefinition.ReportObjects[fieldName] as FieldObject;
                if (fobj != null)
                {
                    fobj.Left = CurrentLeft;
                    fobj.Width = FieldSpace * widths[i] / 100;
                    if (IsNumber(fieldType))
                    {
                        fobj.ObjectFormat.HorizontalAlignment = Alignment.RightAlign;
                        fobj.FieldFormat.NumericFormat.RoundingFormat = RoundingFormat.RoundToTen;
                    }
                    else if (IsBool(fieldType))
                    {
                        fobj.FieldFormat.BooleanFormat.OutputType = BooleanOutputType.YesOrNo;
                    }

                }

                //Headers
                var ColumnHeaderName = headers[i];
                var columnName = "Column" + (i + 1);
                var tobj = report.ReportDefinition.ReportObjects[columnName] as TextObject;
                if (tobj != null)
                {
                    tobj.Text = ColumnHeaderName;
                    tobj.Left = CurrentLeft;
                    tobj.Width = FieldSpace * widths[i] / 100;
                    CurrentLeft = CurrentLeft + tobj.Width + blank;
                    if (IsNumber(fieldType))
                        tobj.ObjectFormat.HorizontalAlignment = Alignment.RightAlign;
                }

            }

A: Well,
since nobody could help me with a solution, I figured one out for my self. I converted booleans and numbers into strings using the toString method. Not the best solution (in my eyes), cause I wanted to change the type of the field object it self, but it works.
                var DbFieldName = mappingNames[i];
                var fieldType = comparisonObj.GetFieldType(DbFieldName);

                var fieldName = "Field" + (i + 1);
                var ffld = report.DataDefinition.FormulaFields[fieldName];
                if (ffld != null)
                {
                    if (IsNumber(fieldType))
                        ffld.Text = "ToText({" + aliasName + "." + DbFieldName + "}, 00, '')";
                    else if (IsDateTime(fieldType))
                        ffld.Text = "ToText({" + aliasName + "." + DbFieldName + "}, \"dd/MM/yyyy\")";
                    else if (IsBool(fieldType))
                        ffld.Text = "if{" + aliasName + "." + DbFieldName + "} then \"Y\" else \"N\"";
                    else
                    {
                        ffld.Text = "{" + aliasName + "." + DbFieldName + "}";
                    }
                }


The Cottages at Thatched Farm Self-cateringThatched Farm is a charming pink washed Grade II Listed property surrounded by open countryside between Waldringfield & Woodbridge.
Daffodil Cottage - Sleeps 3/4: A onetime old farm building now sympathetically renovated whilst retaining its original character & charm. Ground floor with newly fitted kitchen includes fridge, electric oven, hob & microwave. Open plan living/dining area with comfortable seating, TV, DVD. Separate newly fitted shower room. Up the winding oak staircase is a very pretty galleried mezzanine double bedroom. Separate steep staircase to the single mezzanine bedroom (the stairs are not suitable for children under 7 or anyone with mobility difficulties). A sofa bed is also available in the living area.
Bluebell Studio - Sleeps 2, ground floor. The newly fitted kitchen with fridge, electric oven, hob, microwave & breakfast bar opens into the studio double sleeping/living area with French windows onto the gardens.
On site laundry facilities available for both cottages in the separate laundry room with washing machine, tumble dryer, iron & ironing board. Each cottage has its own garden table/chairs & guests are free to use & enjoy the gardens. Heating, linen, towels & tea towels are provided. Ample parking & secure cycle storage available. Sorry, no smoking in the cottages. Children welcome. Both cottages booked together would be ideal for a party of 5/6. Free wireless internet access.
The Cottages at Thatched Farm offers weekly bookings starting on Friday and sometimes short breaks.
1 bed/sitting room with double bed.
Bluebell Studio offers weekly bookings starting on Friday and sometimes short breaks.
Situated 5 minutes from the A12.
Situated 20 minutes from Ipswich Railway Station.
Wonderful stay at a very nice spot.


text:
You are at:Home»Arts and Media»River Campus»Music»Jazz Series to Present Final Concert Performance, 'Hooked On Jazz!'
Jazz Series to Present Final Concert Performance, 'Hooked On Jazz!'
By southeast on	 April 5, 2012 Music
CAPE GIRARDEAU, Mo., April 5, 2012 — Southeast Missouri State University's Department of Music will present its final concert of the 2011-2012 Jazz Series, "Hooked on Jazz!" which will feature Marie Meunier, jazz vocalist, at 7:30 p.m. Thursday, April 19, in the Donald C. Bedell Performance Hall, located in the Cultural Arts Center at Southeast's River Campus.
Meunier, a sophomore music major at Southeast, will perform with both bands during this concert, which will feature music for the big band, which traditionally includes saxophones, trumpets, trombones and a rhythm section.
Studio Jazz Ensemble
Southeast's premier big band, the Studio Jazz Ensemble, returns to the stage to play some of their favorites, including "Hay Burner," from the Count Basie Orchestra, "Cherry Juice," from the Thad Jones/Mel Lewis Orchestra, "Reunion at Newport," from the Woody Herman Orchestra, and "Blue Moan," from the great jazz composer, Don Sebesky, and which features Spencer Day on tenor saxophone. Meunier will be featured in the famous jazz standards, "Nice Work If You Can Get It" and "Almost Like Being in Love." Other featured students include soloist Spencer Day, senior music major; Chris Whited, sophomore music major; saxophonist Corey Easley, graduate psychology major; guitarist Dan Boemeke, senior music major; saxophonist Trevor Smith, sophomore music major; trombonist Matt Gates, senior music major; and bass player Kyle Clay, sophomore music major.
Southeast's Jazz Lab Band will also perform during the concert. This band is comprised of both music and non-music majors and will play a variety of jazz compositions, including "Beantown Blues, Spain," from the Chick Corea Orchestra, "Theme from Sesame Street," from the Maynard Ferguson Orchestra, and "Bye Bye Blackbird." Meunier will also sing with the Lab Band, giving her rendition of "Ain't Misbehavin'" and "For Once in My Life." Other featured performers will be Sam Harris, sophomore computer science major; guitarist Devin Lorenz, freshman music major; tenor sax player Stephen Watkins, freshman music major; and trumpeter Anthony Jackson, freshman music major.
Tickets are on Sale Now.
Tickets may be purchased by contacting the River Campus Box Office, located in the Cultural Arts Center, 518 S. Fountain St., weekdays from 9 a.m. to 4 p.m., or by calling (573) 651-2265, or online at RiverCampusEvents.com.
Southeast River Campus Announces 2019 Summer Arts Festival Event Lineup
Fifth House Ensemble Closes Out 2018-2019 'Sundays at Three' Series with 'Americana'


In 2014, he took a Girona team that had just finished 16th, surviving relegation on the last day, and took them to third, losing out on automatic promotion on the final day. In the very next season, Girona managed to finish fourth, reached the final of the promotion playoff but lost to a Rojillos side - no, not Numancia, Osasuna.
And this season is set to be no different, even though they have somewhat given up on their defensive-first approach, even though this summer 17 players came in and 16 players went out of the club, and even though they lose three of their top four goalscorers. This team plays with flair but more importantly with a determination that makes them chase every loose ball, every 50-50 challenge, and every misplaced pass. Pablo may have retired at 23 but his time as an attacking right back is the personification for Girona's play. This team now plays with more width, just three at the back, and efficient forward passes.
In the Girona vs Numancia game earlier this season, Girona won 3-0. Just look at the first goal - it's a counter attack and Álex Granell, before the halfway line, plays a lofted through ball over the defense to the left wing. It is clearly going out of play, but Sebastián Coris slides in, protects the ball and passes it to his teammate while still on the floor, receives the ball back, crosses it in to Borja García who plays a first-time back-pass-flick-on that even Sergi Roberto would be proud of, and Álex Granell, who makes the lung bursting run from inside his own half to score.
The energy of this Girona side, combined with their unusual three-at-the-back tactics, makes them an even bigger force to reckon this season.
As a club from Soria are plummeting further down the table; a man from Soria is celebrating that, about 300 miles away.


This is deep discussion about reality, science AND art. A long watch, but well worth to investment. Very interested in hearing your take on all of this.
I will be begin by sharing, that while I don't equate my work in sound to relativity, itself. I do think of sound in an atomic manner with the waveform (molecule) and the harmonic (atom) at the core elements of sound. Likewise, I often find the wonderment of these ideas, is a source of inspiration that I try to express in an artistic way.


Modern Carpet Flooring Floor Carpets, Innovative Design Ideas For Style, Comfort And Joy was posted in December 3, 2017 at 5:18 am. Modern Carpet Flooring Floor Carpets, Innovative Design Ideas For Style, Comfort And Joy has been viewed by 179 users. If you want to use this wallpaper, please click the download link below to go to the download page. Right click the image and select "Save Image As" to download the Modern Carpet Flooring Floor Carpets, Innovative Design Ideas For Style, Comfort And Joy to your computer or select "Set Desktop Background As" if your browser has that capability.


text:
Крейчий () — чешская фамилия.

Известные носители:
 Крейчий, Карел (род. 1968) — чешский футболист и футбольный тренер.

 Крейчий, Рихард (род. 1970) — чешский гребец.
 Крейчий, Франтишек (1867—1941) — чешский писатель.

См. также
 Крейчи — неправильная передача фамилии на русский язык.


Charming 13x30mm 18K gold-plated clasp inlaid with shiny zircons as decoration. The well-designed clasp features splendid luster and exquisite craft. It can perfectly serve as a convenient and solid connection of all kinds of quality jewelries. You're welcome to purchase in bulk. Embellish your necklace or bracelet with this delicate clasp and burst forth your grace to the full.


Noami House & Jacksplace Hospice provides expert hospice care to more than 525 life limited and life threatened children, you d adults and their extended families. We are hosting a Easter Egg hunt with quizzes and competitions, games, Bbq, music, raffles and more. Easter Sat @ 3pm, The Crown Inn, Theale Reading.
The event runs from 3:00pm to 11:55pm on the following dates.


Crafty Lumberjacks: Chalk board leaves!
A couple of years ago we made a ton of paper leaves.
After cutting out so many leaves out there was an abundance of leaf stencils.
Inside each stencil we used different colored chalk, then smudged the colors together with our fingers!
Mmmm...breath in that autumn air!
Here are some more leafy ideas!
This is just adorable. Fall is in the air and blowing in through the door. Really great idea.
Love this so much guys!! Thanks for sharing at the party last week!
Sriracha. Maple. Bacon. Cheese. Ball.


text:
Home CDMX News A third audit reveals that the CDMX Metro accident was due to...
A third audit reveals that the CDMX Metro accident was due to lack of maintenance
An auditor's third report on a deadly Mexico City metro accident last year for the first time identified maintenance shortcomings as one of four causes of the collapse of the rail overpass, according to an unpublished version of the study seen by Reuters.
A Mexico City official familiar with the document confirmed the authenticity of the report by Norwegian firm DNV, which was produced at the behest of the city's government to determine the cause of the crash that killed 26 people.
It was meant to be the last of the auditor's three reports since the May 3, 2021, crash on the Line 12 metro service, and it was the first one to cite maintenance as a main cause, according to a Reuters review of the studies published by the city.
Spanish newspaper El Pais published details of the report on Monday. Reuters was unable to establish who leaked the report and for what reason.
The report identifies what it calls four "barriers" which, had they been in place, would have prevented the overpass collapse. Three related to the design, construction and process of supervising the works.
The last point addressed maintenance. In its findings, DNV said it had received "no evidence" that the required inspections on the metro line were performed.
According to the DNV report, the maintenance manual for Line 12 stipulates that inspections of the structure are carried out quarterly, semi-annually, and annually depending on how old the components are.
"The failure to conduct inspections of the viaduct and adhere to the inspection requirements of the maintenance manual is a root cause of the failure," the final report states.
Who exactly is responsible for performing the inspection work was "not clearly specified," it said.
What caused the accident is politically sensitive because the line was built when Foreign Minister Marcelo Ebrard was mayor of the city from 2006 to 2012, and it collapsed 2 1/2 years into the term of the current mayor, Claudia Sheinbaum.
The two are among the top contenders to become Mexico's next president when elections are held in 2024, opinion polls show.
The report has yet to be made public because Sheinbaum's administration – which hired DNV – has rejected the findings, saying they were biased and the methodology flawed.
Sheinbaum, who entered city hall in December 2018, told a news conference on Monday the final DNV report had a "political bias."
To support her claim, she reiterated her assertion that the firm used a lawyer prejudiced against the government to help craft the report, and said that DNV had leaked the findings, breaching a confidentiality agreement.
The city previously identified the lawyer as Hector Salomon Galindo, who held jobs in the previous government. He could not be reached for comment.
Sheinbaum also said on Monday maintenance of the line had not been a problem and that the inspection logs were public.
DNV last week in a statement defended its findings and methodology, and responded to Sheinbaum's assertions about the lawyer by saying the report was produced without the involvement of anyone with a conflict of interest.
DNV did not respond to a request for comment on Monday. It also did not respond to repeated requests for comment on details of the leaked study or why maintenance issues were not flagged in the earlier reports made public by the city.
Sheinbaum, who last week called the report "deficient, poorly executed, with technical problems, tendentious and wrong," said the city was in the process of terminating DNV's contract and filing a lawsuit against the firm because it had diverged from the methodology agreed in its contract.
Asked for comment about the leaked report's findings ahead of Monday's news conference, the mayor's office said the briefing would set out the city's position.
Ebrard's ministry said it had no comment on the media reports. The office of his successor as mayor, Miguel Mancera, did not immediately reply to a request for comment.
Although the report became public this week, the copy seen by Reuters was dated Oct. 28. Reuters was unable to ascertain whether it was later revised.
CDMX Metro
mexico city cdmx
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Is Tesla considering opening a plant near AMLO's AIFA airport?
David Beckham eats insect tacos during his visit to Mexico
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Home NY Times For One Far-Right Politician, Forgetting Germany's Past Just Got Harder
For One Far-Right Politician, Forgetting Germany's Past Just Got Harder
Posted 2 years ago | 0 comments
The memorial has put an uncomfortable national spotlight on Bornhagen, a village of 309 inhabitants on the former border of East and West Germany in the fabled rolling hills of Thuringia, where the Brothers Grimm long ago collected fairy tales.
Mr. Höcke, right, in January. He called on Germans to make a "180 degree" turn in the way they viewed their history. Credit Carsten Koall/European Pressphoto Agency
Mr. Höcke, a history teacher turned local chief of the Alternative for Germany, or AfD, has called the village his "refuge" and "locus of inspiration." But behind its idyllic facade, Bornhagen reflects the new, confusing reality of a Germany where polite political consensus and demographic homogeneity are breaking down, and where the intimate and ideological live side by side.
The far-right AfD won 34 percent of the vote in Bornhagen during the most recent elections, more than twice the national average. But neighbors are neighbors. Supporters of the left-leaning Greens go hunting with AfD voters here. Recent refugees live peacefully in the village, too.
Until the memorial appeared overnight on Nov. 22, these contradictions had coexisted peacefully — at least on the surface. But the art installation has upset village life. For many, it has forced a conversation they would have preferred to avoid.
"We don't talk about politics," said a friend of Mr. Höcke's wife, who like many villagers did not want her name used.
But Mr. Höcke's remarks, which had shocked even some in his own party though it decided not to expel him, have made that mind-your-own-business custom harder to abide for some. And they have raised a challenging question that goes far beyond this village: How should Germans deal with the rise of the far-right, now that an openly nationalist party has entered Parliament for first time since World War II?
For Mr. Ruch and his fellow artists, the answer is clear: People like Mr. Höcke deserve "not one millimeter."
"He broke the mother of all taboos, he challenged the founding narrative of modern Germany and he got away with it," said Mr. Ruch, who born in Dresden and now lives in Berlin.
The memorial has put a national spotlight on Bornhagen. Police cars now patrol the village at all hours. Credit Gordon Welters for The New York Times
"We learn from our past so it does not happen again," he added. "That is who we are. That is our strength."
For 10 months, the artists discreetly scouted out the village. They registered a business to get local license plates, rented the property next to Mr. Höcke's house, and erected a tent in the garden to put together the replica memorial while shielded from view. Neighbors were told the tent was hiding a surprise for an engagement party.
"Aggressive humanists," the artists call themselves. "Terrorists," Mr. Höcke calls them. He has tried to have them evicted, so far to no avail: Their rental contract runs until 2022.
In recent weeks, school classes came to visit the memorial, far-right opponents slashed the tires of cars parked outside the site, and far-left supporters held an anti-fascist march. Death threats have been traded. Police cars now patrol the normally sleepy streets at all hours.
Already, the local butcher says sales of his prizewinning sausages are down. Wedding reservations in the village castle have been put on hold. The only hotel worries about cancellations.
Overnight, said Silvia Rinke, who has voted for the left-leaning Greens all her life, "we have become the Nazi village."
Ms. Rinke, who offers guided tours in medieval garb, says tourists are asking less about the region's rich history, and more about Mr. Höcke. "It's almost embarrassing to say you live here," she said.
Mario Apel, the mayor of Bornhagen, has not visited the memorial. "Bornhagen is not Höcke," he said. Credit Gordon Welters for The New York Times
Bornhagen knows about German history. Its castle is a thousand years old. Goethe and Bismarck are said to have come through. When Ms. Rinke moved to the village in 1993, the old East-West border fence ran through what is now her back yard.
She is appalled by Mr. Höcke's comments. But she is also appalled by some of the artists' methods.
Filming the Höcke family home, for example, or rifling through their trash to get a DNA sample in order to determine Mr. Höcke's own racial purity. (It turns out he has Polish and Portuguese blood.)
This month, villagers found a letter in their mailboxes offering 5,000 euros, or about $ 5,900, to anyone willing to provide a witness statement that Mr. Höcke has written under a pseudonym for a newsletter associated with a convicted neo-Nazi who lives two villages away.
All of that revives memories of a time most people in a village on the border of the former East Germany would rather forget. "I am anti-AfD, but even I feel like I had to become pro-Höcke," said Ms. Rinke, for whom privacy is a red line.
Mr. Ruch insists that the "surveillance" of Mr. Höcke's home was mostly for show. "We are not the state, we are not the Stasi," Mr. Ruch said, referring to the East German secret police. "The state probably should monitor a known Nazi, but it does not."
But no one in Bornhagen is amused. Mario Apel, the mayor, spent his whole life in Bornhagen.
He has not visited the memorial and will not comment on it directly — except to say: "Bornhagen is not Höcke."
Silvia Rinke, right, visited the miniature memorial for the first time this month. Martin Röhrig, left, said he was no fan of Mr. Höcke but knew many people who have embraced the AfD. Credit Gordon Welters for The New York Times
The village has always integrated foreigners, Mr. Apel said. The same barracks that once hosted the border guards of Communist Germany now host 80 asylum seekers from countries like Afghanistan, Iran and Iraq. Three refugees work in the local inn. "There has never been trouble," Mr. Apel said.
Rexhino Metushi, who came to Bornhagen from Albania in 2015 and is doing an apprenticeship as a cook, confirmed this. "I know everybody here and everybody knows me," Mr. Metushi, 18, said in fluent German. The innkeeper, Martin Röhrig, is helping him to get work visas for his parents.
Mr. Röhrig is no fan of the AfD. When Mr. Höcke approached him once to sign a petition, he politely declined. But he knows that several of his clients have embraced the party.
"I don't think that all the people who vote for the AfD are Nazis," said Mr. Röhrig, 34. Many, he said, simply feel abandoned by mainstream parties that "can't even form a government."
Ms. Rinke visited the miniature memorial for the first time last week. She did not stay long. One of her friends lives next door. Her 8-year-old's room overlooks the site.
Children are asking "why people are being mean to Mr. Höcke," Ms. Rinke said. Her son goes to school with his children. "How do you even begin to explain this?"
But that, said Susanne Prinz, a 30-year-old farmer from a neighboring village and one of the volunteers who show the site to visitors, is the point of the memorial: to force people to take a stance.
"You can't just look the other way; that is one lesson we learned from history," she said. "When children ask questions, you have to explain."
"Yes, it's uncomfortable," Ms. Prinz added. "But then, Germany's history is uncomfortable."
Source: NYT > World
Tags: Farright, Forgetting, Germany's, Harder, Just, Politician, пасть
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text:
567 structures 7150 species 3 interactions 7873 sequences 39 architectures
Family: Thymidylat_synt (PF00303)
Summary: Thymidylate synthase
Wikipedia: Thymidylate synthase
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Thymidylate synthase Edit Wikipedia article
TYMS
Available structures
Ortholog search: PDBe RCSB
List of PDB id codes
1YPV, 1JU6, 2RDA, 3EF9, 3ED7, 1HW3, 3EHI, 3GH0, 4JEF, 4O1X, 1HZW, 3EAW, 3OB7, 3EBU, 4KPW, 3EGY, 3EJL, 3N5G, 3N5E, 1HVY, 4GD7, 4G2O, 4H1I, 2ONB, 3H9K, 4GYH, 3HB8, 1I00, 3GG5, 4FGT, 2RD8, 3EDW, 4G6W, 1HW4, 3GH2, 4E28, 1JUJ, 4O1U, 4UP1, 5HS3,%%s1HW3, 1YPV, 4GD7, 2ONB
TYMS, HST422, TMS, TS, thymidylate synthetase
External IDs
OMIM: 188350 MGI: 98878 HomoloGene: 834 GeneCards: TYMS
Gene location (Human)
Chr.
Chromosome 18 (human)[1]
18p11.32
657,653 bp[1]
Gene location (Mouse)
Chromosome 5 (mouse)[2]
5 B1|5 15.81 cM
30,058,202 bp[2]
RNA expression pattern
More reference expression data
• methyltransferase activity
• transferase activity
• nucleotide binding
• protein homodimerization activity
• folic acid binding
• thymidylate synthase activity
• drug binding
• cofactor binding
• mRNA binding
• translation repressor activity, nucleic acid binding
• sequence-specific mRNA binding
• cytoplasm
• cytosol
• membrane
• mitochondrial matrix
• nucleolus
• mitochondrion
• mitochondrial inner membrane
• cell nucleus
• response to cytokine
• nucleotide biosynthetic process
• DNA biosynthetic process
• response to organic cyclic compound
• response to progesterone
• tetrahydrofolate interconversion
• aging
• response to glucocorticoid
• immortalization of host cell by virus
• regulation of transcription involved in G1/S transition of mitotic cell cycle
• dTTP biosynthetic process
• tetrahydrofolate metabolic process
• response to vitamin A
• cartilage development
• methylation
• developmental growth
• response to organophosphorus
• circadian rhythm
• uracil metabolic process
• dTMP biosynthetic process
• intestinal epithelial cell maturation
• dUMP metabolic process
• response to folic acid
• response to ethanol
• response to toxic substance
• response to drug
• pyrimidine nucleobase metabolic process
• regulation of translation
• liver regeneration
• nucleobase-containing small molecule interconversion
• negative regulation of translation
Sources:Amigo / QuickGO
Human Mouse
Q53Y97
RefSeq (mRNA)
RefSeq (protein)
Location (UCSC)
Chr 18: 0.66 – 0.67 Mb Chr 5: 30.06 – 30.07 Mb
PubMed search
View/Edit Human View/Edit Mouse
PDB structures
RCSB PDB PDBe PDBsum
AmiGO / QuickGO
Thymidylat_synt
PROSITE
PDOC00086
1tys / SUPFAM
Available protein structures:
structures / ECOD
RCSB PDB; PDBe; PDBj
PDBsum
Thymidylate synthase (TS) (EC 2.1.1.45)[5] is an enzyme that catalyzes the conversion of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP). Thymidine is one of the nucleotides in DNA. With inhibition of TS, an imbalance of deoxynucleotides and increased levels of dUMP arise. Both cause DNA damage.[6][7]
1 Function
2 Clinical significance
3 Using TS as a drug target
4 Interactive pathway map
5 Mechanism description
The following reaction is catalyzed by thymidylate synthase:
5,10-methylenetetrahydrofolate + dUMP ⇌ {\displaystyle \rightleftharpoons } dihydrofolate + dTMP
By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product.
This provides the sole de novo pathway for production of dTMP and is the only enzyme in folate metabolism in which the 5,10-methylenetetrahydrofolate is oxidised during one-carbon transfer.[8] The enzyme is essential for regulating the balanced supply of the 4 DNA precursors in normal DNA replication: defects in the enzyme activity affecting the regulation process cause various biological and genetic abnormalities, such as thymineless death.[9] The enzyme is an important target for certain chemotherapeutic drugs. Thymidylate synthase is an enzyme of about 30 to 35 kDa in most species except in protozoan and plants where it exists as a bifunctional enzyme that includes a dihydrofolate reductase domain.[8] A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate). The sequence around the active site of this enzyme is conserved from phages to vertebrates.
Thymidylate synthase is induced by a transcription factor LSF/TFCP2 and LSF is an oncogene in hepatocellular carcinoma. LSF and Thymidylate synthase plays significant role in Liver Cancer proliferation and progression and Drug resistance.[10]
Thymidylate synthase (TS) plays a crucial role in the early stages of DNA biosynthesis.[11] DNA damage or deletion occur on a daily basis as a result of both endogenous and environmental factors. Such environmental factors include ultraviolet damage and cigarette smoke that contain a variety of carcinogens.[12] Therefore, synthesis and insertion of healthy DNA is vital for normal body functions and avoidance of cancerous activity. In addition, inhibition in synthesis of important nucleotides necessary for cell growth is important. For this reason, TS has become an important target for cancer treatment by means of chemotherapy. The sensitivity of TS to succumb to TS inhibitors is a key part to its success as treatment for colorectal, pancreatic, ovarian, gastric, and breast cancers.[11]
Using TS as a drug target
The use of TS inhibitors has become a main focus of using TS as a drug target. The most widely used inhibitor is 5-fluorouracil (5-FU), which acts as an antimetabolite that irreversibly inhibits TS by competitive binding.[13][14] However, due to a low level of 5-FU found in many patients, it has been discovered that in combination with leucovorin (LV), 5-FU has greater success in down regulating tumor progression mechanisms and increasing immune system activity.[15]
Experimentally, it has been shown that low levels of TS expression leads to a better response to 5-FU and higher success rates and survival of colon and liver cancer patients.[11] However, additional experiments have merely stated that levels of TS may be associated with stage of disease, cell proliferation and tumor differentiation for those with lung adenocarcinoma but low levels are not necessarily indicators of high success. Expression levels of TS mRNA may be helpful in predicting the malignant potential of certain cancerous cells, thus improving cancer treatment targets and yielding higher survival rates among cancer patients [Hashimoto].
TS's relation to the cell cycle also contributes to its use in cancer treatment. Several cell-cycle dependent kinases and transcription factors influence TS levels in the cell cycle that increase its activity during the S phase but decrease its activity while cells are no longer proliferating.[11] In an auto-regulatory manner, TS not only controls its own translation but that of other proteins such as p53 that through mutation is the root of much tumor growth. Through its translation, TS has a varying expression in cancer cells and tumors, which leads to early cell death.[14]
Interactive pathway map
Click on genes, proteins and metabolites below to link to respective articles.[§ 1]
[[File:
[[
|{{{bSize}}}px|alt=Fluorouracil (5-FU) Activity edit]]
Fluorouracil (5-FU) Activity edit
^ The interactive pathway map can be edited at WikiPathways: "FluoropyrimidineActivity_WP1601".
Mechanism description
This shows the overall pathway that thymidylate synthase and its intermediates take
In the proposed mechanism, TS forms a covalent bond to the substrate dUMP through a 1,4-addition involving a cysteine nucleophile. The substrate tetrahydrofolate donates a methyl group to the alpha carbon while reducing the new methyl on dUMP to form dTMP.[16]
This shows the intricate interactions of the UMP in the active site of thymidylate synthase.
It has been proven that the imine formed through reaction with THF and dUMP is an intermediate in the reaction with dUMP through mutations in the structure of TS that inhibit the completion of the mechanism. V316Am TS, a mutant with deletion of C terminal valines from both subunits, allows the catalysis of dehalogenation of BrdUMP preceding the mechanism described above and the covalent bond to THF and dUMP. The mutant TS is unable to accomplish the C-terminal conformational change needed to break covalent bonds to form dTMP, thus showing the proposed mechanism to be true. The structure was deduced through x-ray crystallography of V316Am TS to illustrate full homodimer TS structure (Figure 1). In addition, it showed possible interactions of the 175Arg and 174Arg between dimers. These arginines are thought to stabilize the UMP structures within the active sites by creating hydrogen bonds to the phosphate group (Figure 2). [Stroud and Finer-Moore][citation needed] 5-FU is an inhibitor of TS. Upon entering the cell, 5-fluorouracil (5-FU) is converted to a variety of active metabolites, intracellularly. One such metabolite is FdUMP which differs from dUMP by a fluorine in place of a hydrogen on the alpha carbon. FdUMP is able to inhibit TS by binding to the nucleotide-binding site of dUMP. This competitive binding inhibits the normal function of dTMP synthesis from dUMP [Longley].[citation needed] Thus the dUMP is unable to have an elimination reaction and complete the methyl donation from THF.
Thymidylate synthase as a homodimer
Figure 1. This figure depicts the homodimer that is TS. As you can see the orange and teal backbones never connect or intertwine, but there are side chains interactions between the dimers. On the orange protein, you can visibly detect two long side chains that enter the teal protein (this is located within the yellow circle). The other beige parts are side chains that interact within the active site. Just below the yellow circle, you are able to see the same pattern of side chains and configuration.
Active site of Thymidylate Synthase
Figure 2. This figure shows the possible H-bond interactions between the arginines and the UMP in the active site of thymidylate synthase. This can be seen by the faint lines between the blue tips and the red tips. These arginines are used to hold the position of the UMP molecule so that the interaction may occur correctly. The two arginines in the top right corner that are located next to each other on the back bone are actually from the other protein of this dimer enzyme. This interaction is one of the many intermolecular forces that holds these two tertiary structures together. The yellow stand in the top-middle region shows a sulfur bond that forms between a cysteine side chain and UMP. This covalently holds the UMP within the active site until it is reacted to yield TMP.
Pyrimidine analogues
Thymidylate synthase inhibitor
Thymidine kinase
Thymidine kinase in clinical chemistry
Thymidylate kinase
^ a b c GRCh38: Ensembl release 89: ENSG00000176890 - Ensembl, May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000025747 - Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Entrez Gene: TYMS thymidylate synthetase".
^ "DNA: Form and Function" (PDF).
^ "DNA Synthesis".
^ a b Stroud RM, Santi DV, Hardy LW, Montfort WR, Jones MO, Finer-Moore JS (1987). "Atomic structure of thymidylate synthase: target for rational drug design". Science. 235 (4787): 448–455. Bibcode:1987Sci...235..448H. doi:10.1126/science.3099389. PMID 3099389.
^ Gotoh O, Shimizu K, Kaneda S, Nalbantoglu J, Takeishi K, Seno T, Ayusawa D (1990). "Structural and functional analysis of the human thymidylate synthase gene". J. Biol. Chem. 265 (33): 20277–20284. PMID 2243092.
^ Santhekadur PK, Rajasekaran D, Siddiq A, Gredler R, Chen D, Schaus SE, Hansen U, Fisher PB, Sarkar D (2012). "The transcription factor LSF: a novel oncogene for hepatocellular carcinoma" (PDF). Am J Cancer Res. 2 (3): 269–85. PMC 3365805. PMID 22679558.
^ a b c d Peters GJ, Backus HH, Freemantle S, van Triest B, Codacci-Pisanelli G, van der Wilt CL, Smid K, Lunec J, Calvert AH, Marsh S, McLeod HL, Bloemena E, Meijer S, Jansen G, van Groeningen CJ, Pinedo HM (2002). "Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism". Biochim. Biophys. Acta. 1587 (2–3): 194–205. doi:10.1016/S0925-4439(02)00082-0. PMID 12084461.
^ "Leucovorin". MedlinePlus Drug Information. U.S. National Library of Medicine.
^ Papamichael D (2000). "The Use of Thymidylate Synthase Inhibitors in the Treatment of Advanced Colorectal Cancer: Current Status". The Oncologist. 4 (6): 478–87. PMID 10631692.
^ a b Papamichael D (1999). "The use of thymidylate synthase inhibitors in the treatment of advanced colorectal cancer: current status". Oncologist. 4 (6): 478–87. PMID 10631692.
^ Nicolini A, Conte M, Rossi G, Ferrari P, Duffy M, Barak V, Carpi A, Miccoli P (2011). "Additional 5-FU-LV significantly increases survival in gastrointestinal cancer". Front Biosci. 3: 1475–82. doi:10.2741/348. PMID 21622151.
^ Carreras CW, Santi DV (1995). "The catalytic mechanism and structure of thymidylate synthase". Annu. Rev. Biochem. 64: 721–62. doi:10.1146/annurev.bi.64.070195.003445. PMID 7574499.
Carreras CW, Santi DV (1995). "The Catalytic Mechanism and Structure of Thymidylate Synthase". Annual Review of Biochemistry. 64 (1): 721–762. doi:10.1146/annurev.bi.64.070195.003445. PMID 7574499.
Banerjee D, Mayer-Kuckuk P, Capiaux G, et al. (2002). "Novel aspects of resistance to drugs targeted to dihydrofolate reductase and thymidylate synthase". Biochim. Biophys. Acta. 1587 (2–3): 164–73. doi:10.1016/S0925-4439(02)00079-0. PMID 12084458.
Liu J, Schmitz JC, Lin X, et al. (2002). "Thymidylate synthase as a translational regulator of cellular gene expression". Biochim. Biophys. Acta. 1587 (2–3): 174–82. doi:10.1016/s0925-4439(02)00080-7. PMID 12084459.
Chu J, Dolnick BJ (2002). "Natural antisense (rTSalpha) RNA induces site-specific cleavage of thymidylate synthase mRNA". Biochim. Biophys. Acta. 1587 (2–3): 183–93. doi:10.1016/s0925-4439(02)00081-9. PMID 12084460.
Peters GJ, Backus HH, Freemantle S, et al. (2002). "Induction of thymidylate synthase as a 5-fluorouracil resistance mechanism". Biochim. Biophys. Acta. 1587 (2–3): 194–205. doi:10.1016/S0925-4439(02)00082-0. PMID 12084461.
Costi MP, Tondi D, Rinaldi M, et al. (2002). "Structure-based studies on species-specific inhibition of thymidylate synthase". Biochim. Biophys. Acta. 1587 (2–3): 206–14. doi:10.1016/s0925-4439(02)00083-2. PMID 12084462.
Lin D, Li H, Tan W, et al. (2007). Genetic polymorphisms in folate- metabolizing enzymes and risk of gastroesophageal cancers: a potential nutrient-gene interaction in cancer development. Forum of Nutrition. 60. pp. 140–5. doi:10.1159/000107090. ISBN 978-3-8055-8216-2. PMID 17684410.
Thymidylate+synthetase at the US National Library of Medicine Medical Subject Headings (MeSH)
PDBe-KB provides an overview of all the structure information available in the PDB for Human Thymidylate synthase
PDB gallery
1hvy: Human thymidylate synthase complexed with dUMP and Raltitrexed, an antifolate drug, is in the closed conformation
1hw3: STRUCTURE OF HUMAN THYMIDYLATE SYNTHASE SUGGESTS ADVANTAGES OF CHEMOTHERAPY WITH NONCOMPETITIVE INHIBITORS
1hw4: STRUCTURE OF THYMIDYLATE SYNTHASE SUGGESTS ADVANTAGES OF CHEMOTHERAPY WITH NONCOMPETITIVE INHIBITORS
1hzw: CRYSTAL STRUCTURE OF HUMAN THYMIDYLATE SYNTHASE
1i00: CRYSTAL STRUCTURE OF HUMAN THYMIDYLATE SYNTHASE, TERNARY COMPLEX WITH DUMP AND TOMUDEX
1ju6: Human Thymidylate Synthase Complex with dUMP and LY231514, A Pyrrolo(2,3-d)pyrimidine-based Antifolate
1juj: Human Thymidylate Synthase Bound to dUMP and LY231514, a Pyrrolo(2,3-d)pyrimidine-based Antifolate
1ypv: Structure of human thymidylate synthase at low salt conditions
2onb: Human Thymidylate Synthase at low salt conditions with PDPA bound
Metabolism: amino acid metabolism
nucleotide enzymes
Anabolism
R5P→IMP:
Ribose-phosphate diphosphokinase
Amidophosphoribosyltransferase
Phosphoribosylglycinamide formyltransferase
AIR synthetase (FGAM cyclase)
Phosphoribosylaminoimidazole carboxylase
Phosphoribosylaminoimidazolesuccinocarboxamide synthase
IMP synthase
IMP→AMP:
Adenylosuccinate synthase
Adenylosuccinate lyase
AMP deaminase
IMP→GMP:
IMP dehydrogenase
GMP synthase
GMP reductase
Nucleotide salvage
Hypoxanthine-guanine phosphoribosyltransferase
Adenine phosphoribosyltransferase
Catabolism
Purine nucleoside phosphorylase
Guanine deaminase
Xanthine oxidase
Urate oxidase
Pyrimidine metabolism
Carbamoyl phosphate synthase II
Aspartate carbamoyltransferase
Dihydroorotase
Dihydroorotate dehydrogenase
Orotidine 5'-phosphate decarboxylase/Uridine monophosphate synthetase
CTP synthetase
Dihydropyrimidine dehydrogenase
Dihydropyrimidinase/DPYS
Beta-ureidopropionase/UPB1
Deoxyribonucleotides
Ribonucleotide reductase
Nucleoside-diphosphate kinase
DCMP deaminase
Dihydrofolate reductase
Transferase: one carbon transferases (EC 2.1)
2.1.1: Methyl-
N-
Histamine N-methyltransferase
Phenylethanolamine N-methyltransferase
Amine N-methyltransferase
Phosphatidylethanolamine N-methyltransferase
5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase
Catechol-O-methyl transferase
Betaine-homocysteine methyltransferase
Homocysteine methyltransferase
Methionine synthase
Phosphatidyl ethanolamine methyltransferase
DNMT3B
Thiopurine methyltransferase
Cyclopropane-fatty-acyl-phospholipid synthase
2.1.2: Hydroxymethyl-,
Formyl- and Related
Hydroxymethyltransferase
Serine hydroxymethyltransferase
3-methyl-2-oxobutanoate hydroxymethyltransferase
Formyltransferase
Inosine monophosphate synthase
Glutamate formimidoyltransferase
Aminomethyltransferase
2.1.3: Carboxy-
and Carbamoyl
methylmalonyl-CoA carboxytransferase
Carbamoyl
Ornithine carbamoyltransferase
Oxamate carbamoyltransferase
Putrescine carbamoyltransferase
3-hydroxymethylcephem carbamoyltransferase
Lysine carbamoyltransferase
N-acetylornithine carbamoyltransferase
2.1.4: Amidine
Arginine:glycine amidinotransferase
Active site
Binding site
Catalytic triad
Oxyanion hole
Enzyme promiscuity
Catalytically perfect enzyme
Enzyme catalysis
Cooperativity
Enzyme inhibitor
Enzyme activator
Enzyme superfamily
Enzyme family
List of enzymes
Eadie–Hofstee diagram
Hanes–Woolf plot
Lineweaver–Burk plot
Michaelis–Menten kinetics
EC1 Oxidoreductases (list)
EC2 Transferases (list)
EC3 Hydrolases (list)
EC4 Lyases (list)
EC5 Isomerases (list)
EC6 Ligases (list)
EC7 Translocases (list)
Biology portal
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
Thymidylate synthase Provide feedback
This is a family of proteins that are flavin-dependent thymidylate synthases.
Myllykallio H, Lipowski G, Leduc D, Filee J, Forterre P, Liebl U;, Science. 2002;297:105-107.: An alternative flavin-dependent mechanism for thymidylate synthesis. PUBMED:12029065 EPMC:12029065
Leduc D, Graziani S, Meslet-Cladiere L, Sodolescu A, Liebl U, Myllykallio H;, Biochem Soc Trans. 2004;32:231-235.: Two distinct pathways for thymidylate (dTMP) synthesis in (hyper)thermophilic Bacteria and Archaea. PUBMED:15046578 EPMC:15046578
Leduc D, Escartin F, Nijhout HF, Reed MC, Liebl U, Skouloubris S, Myllykallio H;, J Bacteriol. 2007;189:8537-8545.: Flavin-dependent thymidylate synthase ThyX activity: implications for the folate cycle in bacteria. PUBMED:17890305 EPMC:17890305
External database links
HOMSTRAD: tms
PRINTS: PR00108
PROSITE: PDOC00086
SCOP: 1tys
Thymidylate synthase (EC) [PUBMED:6996564, PUBMED:2117882] catalyzes the reductive methylation of dUMP to dTMP with concomitant conversion of 5,10-methylenetetrahydrofolate to dihydrofolate: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP This provides the sole de novo pathway for production of dTMP and is the only enzyme in folate metabolism in which the 5,10-methylenetetrahydrofolate is oxidised during one-carbon transfer [PUBMED:3099389]. The enzyme is essential for regulating the balanced supply of the 4 DNA precursors in normal DNA replication: defects in the enzyme activity affecting the regulation process cause various biological and genetic abnormalities, such as thymineless death [PUBMED:2243092]. The enzyme is an important target for certain chemotherapeutic drugs. Thymidylate synthase is an enzyme of about 30 to 35 Kd in most species except in protozoan and plants where it exists as a bifunctional enzyme that includes a dihydrofolate reductase domain [PUBMED:3099389]. A cysteine residue is involved in the catalytic mechanism (it covalently binds the 5,6-dihydro-dUMP intermediate) [PUBMED:16615077,PUBMED:7574499]. The sequence around the active site of this enzyme is conserved from phages to vertebrates.
Thymidylate synthase also acts as a regulator of its own expression by binding and inactivating its own RNA. Due to its key role in the de novo pathway for thymidylate synthesis and, hence, DNA synthesis, it is one of the most conserved enzymes across species and phyla [PUBMED:16162288,PUBMED:16511011]. Thymidylate synthase is a well-recognized target for anticancer chemotherapy, as well as a valuable new target against infectious diseases [PUBMED:16178783]. Interestingly, in several protozoa, a single polypeptide chain codes for both dihydrofolate reductase (DHFR) and thymidylate synthase (TS), forming a bifunctional enzyme (DHFR-TS), possibly through gene fusion at a single evolutionary point. DHFR-TS is also active as a dimer [PUBMED:14555647].
This entry also includes dUMP hydroxymethylases and dCMP hydroxymethyltransferases from bacteriophages. dCMP-HMase catalyzes the reversible conversion of dCMP and CH2THF to hydroxymethyl-dCMP and THF. dUMP hydroxymethylase is encoded by several bacteriophages that infect Bacillus subtilis and contain hydroxymethyl-dUMP instead of dTMP in their DNA, for their own protection against the host restriction system [PUBMED:10064578].
dCMP hydroxymethyltransferase has a subunit fold and a dimerization pattern in common with thymidylate synthases [PUBMED:10064578].
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Seed source: Prosite
Previous IDs: thymidylat_synt;
Author: Finn RD
Number in seed: 538
Average length of the domain: 266.50 aa
Model length: 268
Family (HMM) version: 19
There are 3 interactions for this family. More...
We determine these interactions using iPfam, which considers the interactions between residues in three-dimensional protein structures and maps those interactions back to Pfam families. You can find more information about the iPfam algorithm in the journal article that accompanies the website.
DHFR_1 Thymidylat_synt DHFR_1
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Thymidylat_synt domain has been found. There are 567 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein sequence.
Loading structure mapping...


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A wet day again, but rather than yet another in the succession of drab days with rain falling unceasingly from a low and featureless grey sky, today's wind-driven weather is full of life and drama. The latest shower slams in from across the sea, an amorphous leaden cloud turning the morning dark and releasing a downpour so heavy that we pull off to the side of the road where we share a passing place with the sheep sheltering in the lee of the sea wall.
The rain blows over as quickly as it arrived – a hazy silvery sun gradually becoming visible behind the thinning clouds. In only a few moments the sun emerges completely and, where so recently the land lay under the shadow of the cloud, now all is fresh and bright. The sunlight turns the stark winter reeds blond and spotlights the white walls of houses against the backdrop of the retreating dark cloud that has swept onwards to obscure the hills. A double rainbow arcs across the sky and the sheep amble away from the wall to resume their grazing.
The sun is still shining when we arrive at the start of our walk. Behind the line of dunes the familiar landscape has been transformed by weeks of rain; a new loch has appeared, stretching ahead of us as far as we can see, the parallel indentations that mark the route of tractors along the foot of the dunes disappearing below the waters and then re-emerging a little further along.
Greylag geese feed busily on the grassland between the permanent loch and this new creation, which has already attracted its own visiting whooper swans and tufted duck. We follow the new loch to its end and then climb to the dune ridge – before us the white sands of the beach stretch away to north and south but though the sun still shines, the horizon is already darkening ominously once again.