text:
Buy, hold, sell: Three of Australia's hottest stocks (and two actual buys)
Ally Selby: Hey, how are you doing? And welcome to Livewire's Buy Hold Sell. I'm Ally Selby, and today we have a little bit of a fun one for you.
We're going to be discussing the hottest, the most-discussed stocks in the market, plus we'll also be talking about two stocks that you probably should be looking at instead. To do that, I'm joined by Ben Rundle from Hayborough Investment Partners and Michael Wayne from Medallion Financial.
There's nothing hotter than lithium at the moment, so we better start there. We're going to be talking about Allkem. Michael, over to you. Is it a buy, hold or sell?
Allkem (ASX: AKE)
Michael Wayne (BUY): Going to go buy on Allkem. It's been on a very good trajectory of late. The lithium price has been incredible over the past 12 months, up 300-400 per cent or so. And what I like about AKE is the fact that it's producing a lot now, but it's also got a lot of projects coming online over the next couple of years, which will continue to supply that lithium market as demand grows in the years to come. And I think AKE is well positioned to produce and capitalise on the high prices that should be forthcoming.
Ally Selby: You mentioned there that it's been on quite a good trajectory. Its share price has lifted around 76 per cent over the past year. Ben, over to you. Is it a buy, hold or sell?
Ben Rundle (HOLD): I'll say hold. As Michael says, the lithium price has just gone berserk over the last couple of years and at the current prices, this company is absolutely printing cash. So if it stays there, then the price is going a lot higher. The price so far has been very demand-driven. There will be a supply-side response to that at some point.
I don't think that'll be so great that it quashes demand, but I don't have enough conviction that it won't. So, I'll say hold. But look, if that lithium price stays anywhere close to where it is, then they'll have their market cap in cash pretty soon.
Appen (ASX: APX)
Ally Selby: Next up, we have Appen. It's actually been talked about for all the wrong reasons. Ben, can you take us through it and is it a buy, hold or sell?
Ben Rundle (SELL): Yeah, I think Appen is still a sell. Deteriorating earnings quality and huge customer concentration risk. I think people have worked out pretty quickly the hard way that this doesn't have the same sort of earnings quality as what you would see in a traditional SaaS business. I'm also very aware of companies that put out long-range earnings guidance and I think they've put out guidance for FY26. They can't even hit their short-term guidance. So, I still think it's a sell despite the move lower.
Ally Selby: Okay, over to you Michael. Its share price has moved 70 per cent lower over the past 12 months. Is it a buy, hold or sell?
Michael Wayne (SELL): It's a sell. It's been a fallen darling in every sense of the word. I can't even keep up with how many times they've missed earnings forecasts in recent times. And the business is a lot more capital-intensive than many people would understand. It often is basketed together with all the tech businesses, but there are a lot of people in a room marking different things and inputting data into the computers so that machines can learn.
They've got a very big, contract book. They've actually got more projects in the pipeline than ever before. It's just that those projects are for a lot lower margins compared to some of their old projects. So for whatever reason, a lot of their large customers, although they might be giving them the occasional project or contract, are just not converting at the same margins as in the past.
And because the management team keeps changing their earnings outlook, it's a good indication that no one in that company really has a good grasp of exactly what's going on. So we remain very, very cautious on Appen. The long-term outlook is very clouded and those former heady share prices are something of the past. So I'm going to keep a sell on that.
Whitehaven Coal (ASX: WHC)
Ally Selby: So both our fundies are avoiding Appen. Next up we have Whitehaven Coal. Coal is having a bit of a moment right now and our readers are loving this stock as well. Michael, over to you. Is it a buy, hold or sell?
Michael Wayne (SELL): I'm going to go a sell on Whitehaven Coal. And it's a difficult thing to say with a company on such a good momentum trajectory, but the fact of the matter is, the long-term outlook for coal still remains clouded. I get the narrative that there's not a lot more supply coming online.
Coal companies find it very difficult to get projects over the line, and in the case of Whitehaven, they are doing a share buyback as well. So although I have a long-term sell, in the short term, I wouldn't be surprised if momentum is higher. But I am conscious of the very cyclical nature of the coal price. There's also a difference between thermal coal and coking coal. Whitehaven does have exposure to coking coal, which is used in the production of steel. And I am conscious of the fact that if the global economy does fall into a deep recession, steel prices will probably struggle a fair bit. So I'm going to go a counter-cyclical sell on that.
Ally Selby: Okay, over to you Ben. Its share price has lifted 224 per cent over the past 12 months. Is it a buy, hold or sell?
Ben Rundle (BUY): I'm going to take the other side of that trade and call it a buy. Look, Michael makes some fantastic points. The price of coal has had a huge rally and there are questions on whether it'll stay there at the current price. The company is just absolutely making so much cash flow.
I think that, unlike what we talked about with the lithium names, there's no supply-side response coming in the coal market. So I think that price stays a lot higher for a lot longer. They've announced a 25 per cent buyback of the company. So I think that supports the share price and despite the move, I still think it's a buy.
Aussie Broadband (ASX: ABB)
Ally Selby: Wow. Okay. So those are three stocks that a lot of the market is talking about right now. I want to learn about a stock that no one really is talking about. Maybe they've missed it. What stock have you brought for us today?
Ben Rundle (BUY): Aussie Broadband. I feel like I'm sticking my neck out a little bit with this company because it's trading like it's faced a monster downgrade. I really like businesses that have higher levels of customer satisfaction and Aussie Broadband has that in its residential broadband segment and therefore it's taken a huge amount of market share.
They've pushed into the commercial side of their market and I think if they apply the same model, they'll have the same level of success. At the moment, we're in a bit of a transition phase where nobody's really seeing the fruits of that yet. Hence, the share price has derated quite significantly from where it has been. It's quite cheap for what I think it is. It's a founder-led business that has a fantastic management team. So I think that it's offering a pretty good opportunity to buy it here.
XRF Scientific (ASX: XRF)
Ally Selby: Okay, over to you Michael. What's your underrated gem?
Michael Wayne (BUY): XRF Scientific. It's a smaller business, but it's quite mature. Basically what it does is it provides testing kits to large mining companies in order for them to test their core samples. But once they've sold the machines to those mining companies, they then have to sell the consumables part of that, which is the chemicals to conduct those tests. In recent years, they've developed that technology quite nicely. They've sold a lot of machines to a lot of mining companies, but the fastest-growing part of the business at the moment is actually the consumables part.
That's got the highest margins and it's growing the quickest, and we expect that momentum to continue. So the earnings growth's been very, very good. The dividend on this one's about 3 per cent-3.5 per cent, and it's been a growing dividend at about 20 per cent-25 per cent per year. So it's a high-quality small cap if you like. So it's a good buy for us.
Ally Selby: Okay. Well Michael brought along XRF Scientific and Ben brought along Aussie Broadband, but we'd like to know if there's a stock you think the rest of the market has missed. Let us know in the comments section below. If you enjoyed that video, give it a like and subscribe to our YouTube channel. We're adding new content every week.
Buy Hold Sell is a weekly video series produced by Livewire Markets. This article was first published on Livewire Markets.
Disclaimer: The information contained in this presentation is general in nature and should not be relied upon. Before making any investment or financial planning decisions, you should consult a licensed professional who can advise you on whether the decision is appropriate for you. Contributors to this show may have commercial or financial interests in the companies mentioned.
Appen Limited
Whitehaven Coal
XRF Scientific Limited
Latest In Investing


text:
Q: how to Add new property to system class? using System;

namespace Telerik.Windows.Controls
{
    public class Resource : IResource
    {
        public Resource();
        public Resource(string name);
        public Resource(string name, string type);

        public string DisplayName { get; set; }
        public string ResourceName { get; set; }
        public string ResourceType { get; set; }
    }
}

I want to add a public string NoOfAppointments { get; set; } property to this class to customize Resource Class for my application.
How do i Achieve it ?
Can we define partial class of Resource,if We can how do i define?
After Adding new property I should use new derived or partial class as same as Resource Class.

A: So this isn't working, or?
public class MyResource : Resource
{
    public MyResource();
    public MyResource(string name);
    public MyResource(string name, string type);

    public string NoOfAppointments { get; set; }
}

A: You can't add a property to an existing class. Partial classes won't help you because they're a compile-time feature for source files within the same project - you can't add to a class from a different assembly using partial classes.
You could derive a new class from Resource, as Marius has shown - but then everywhere that you get given just a Resource, you'd have to cast to the right type - and if the Telerik controls copy resource objects without noticing subclasses, that could cause problems. That may or may not actually be an issue for you - it's hard to say without knowing more about what you're trying to do.

A: I don't know what exactly you are trying to achieve, but I'm quite sure that the way you are going about it is incorrect. If you can tell more, what this Resource class is, how you used it and where the NoOfAppointments fits the picture, it would be easier to help.
Telerik is a third party library. It most cases, you can not change definition of classes inside a third party library. You also can not use partial classes for the classes from third party libraries. You can derive you classes from other classes and Marius Bancila just shown you how you do it in his answer. Note that he is not repeating the class from your question but rather defines a new class and specified that it is derived from it: class MyResource : Resource
But you can not force the library to use your class if it is hardwired to use its own class, so most likely deriving will not help you.

A: Finally i used staight forward way do it and its working.
    public class CustomResource: Telerik.Windows.Controls.Resource
    {
        private int noOfAppointments;
        public int NoOfAppointments
        {
            get { return noOfAppointments; }
            set
            {
                if (value > 0)
                    noOfAppointments = value;
                else
                    noOfAppointments = 0;
            }
        }
    }

redpajama_set_name: RedPajamaStackExchange


text:
Goodspeed
Verse:28
and one of them named Agabus got up and under the influence of the Spirit revealed the fact that there was going to be a great famine all over the world. This was the famine that occurred in the reign of Claudius.
One of them named Agabus stood up and began to indicate by the Spirit that there would certainly be a great famine all over the world And this took place in the reign of Claudius.
And there stood up one of them named Agabus, and signified by the spirit that there should be great dearth throughout all the world: which came to pass in the days of Claudius Caesar.
Then one of them, named Agabus, stood up and predicted by the Spirit that there would be a severe famine throughout the Roman world. This took place during the time of Claudius.
One of them named Agabus got up and predicted by the Spirit that there would be a severe famine all over the world. This happened during the reign of Claudius.
And one of them named Agabus, after standing up, signified by the Spirit there was going be a great famine in the whole world, which also happened under Claudius Caesar.
And there stood up one of them named Agabus, and signified by the Spirit that there should be a great famine over all the world: which came to pass in the days of Claudius.
And one of them named Agabus stood up and prophesied through the [Holy] Spirit that a great and severe famine would come upon the whole world. And this did occur during the reign of Claudius.
One of them, a man named Agabus, stood up and indicated by [the direction of] the Holy Spirit that there was going to be a severe worldwide famine, which [actually] took place during the time that Claudius ruled.
And one of them, named Agabus, rose and made known, by the Spirit, that a great famine was about to come on the whole habitable land; which took place in the days of Claudius.
And one of them, named Agabus, said publicly through the Spirit that there would be serious need of food all over the earth: which came about in the time of Claudius.
One of them named Agabus stood up and foretold by the Spirit that there would be a great famine all over the world. And this took place in the reign of Claudius.
and one of them named Agabus foretold by the spirit, that there should be a great famine, thro' all the world: which accordingly happen'd in the reign of Claudius Cesar.
and one from among them, by name Agabus, rose up and signified by the Spirit that there was going to be a great famine over all the inhabited earth, which also came to pass under Claudius.
And one of them by name Agabus, standing up, signified through the Spirit that there is about to be a great famine throughout the whole world: which took place under the reign of Claudius.
named Agabus stood up, and signified by the Spirit, that there would be a great famine through all the world; which also came to pass under Claudius Cesar.
And one of them named Agabus stood up and signified by the Spirit that there should be great famine throughout all the world, which came to pass in the days of Claudius Caesar.
And one of them having risen, Agabus by name, signified by the Holy Spirit a great famine was about to be upon the whole habitable globe: which was during Claudius Caesar.
And there stood up one of them named Agabus, and signified by the Spirit that there should be great famine throughout all the world: which came to pass in the days of Claudius Caesar.
And one of them {named} Agabus stood up [and] indicated by the Spirit [that] a great famine was about to come over the whole inhabited earth (which took place in the time of Claudius).
And one of them named Agabus stood up and signified by the Spirit that there should be great famine over the world (which also happened in the days of Claudius Caesar).
And there stood up one of them named Agabus, and signified by the spirit, that there should be great dearth throughout all the world, which came to pass in the Emperor Claudius' days.
one of whom, named Agabus, showed by the Spirit that a severe famine was about to visit the whole world (the famine which occurred in the reign of Claudius).
One of them, who was Agabus, rose up, and being instructed by the Spirit, predicted that a great famine was about to come upon the whole inhabited earth. (It came in the reign of Claudius.)
One of them, named Agabus, got up and predicted by the Spirit that a severe famine was about to come over the whole inhabited world. (This took place during the reign of Claudius.)
One of them named Agabus stood up, and indicated by the Spirit that there should be a great famine all over the world, which also happened in the days of Claudius.
And one of them named Agabus stood up, and signified by the Spirit, that there was about to be a great famine over the whole world; which came to pass in the days of Claudius.
and one of them, by the name of Agabus, standing up signified by the spirit that a great famine was about to come on all the world; which also came under Claudius Caesar.
And one from among them, by name Agabus, rising up, gave a sign, through means of the Spirit, that, a great famine, was coming over all the inhabited earth; which, indeed, came to pass under Claudius.
And one of them named Agabus, rising up, signified by the spirit that a great famine was coming upon the whole habitable globe: which also came in the reign of Claudius Caesar.
One of them, named Agabus, came forward and, under the influence of the Spirit, foretold a great famine that was to spread over all the world--a famine which occurred in the reign of Claudius.
And there stood up one of them named Agabus, and signified by the Spirit that there should be great dearth throughout all the world: which came to pass in the days of Claudius Cesar.
one of whom, named Agabus, being instructed by the Spirit, publicly predicted the speedy coming of a great famine throughout the world. (It came in the reign of Claudius.)
and one of them named Agabus got up and, through the Holy Spirit, foretold that there was going to be a great famine all over the world, which occurred in the reign of Claudius.
and one of them??gabus by name??tanding up, signified through the Spirit that there was about to be a great famine over all the inhabited earth; which, indeed, happened under Claudius.
And one of them, named Agabus, stood up and signified by the Spirit, that there would be a great famine over all the earth: which accordingly came to pass in the reign of Claudius Cesar.
and one of them, by name Agabus, having stood up, did signify through the Spirit a great dearth is about to be throughout all the world -- which also came to pass in the time of Claudius Caesar --
Agabus » A prophet
Antioch » A city » A city of syria » Disciples first called Christians in
Caesar » Claudius » Caesar claudius
Caesar » Claudius
Claudius » Emperor of rome
Famine » Prophesied
Famine » Instances of, in scripture » In the reign of claudius cæsar
Famine » Instances of » Universal
Inspiration » Examples of » Ezekiel
Paul » Conveys the contributions of the Christians in antioch to the Christians in jerusalem
Prophecy » They who uttered » Spake by the holy ghost
Prophets » Examples of » Ezekiel
Prophets » Mentioned in scripture » Agabus
Roman emperors » Caesar claudius
The Roman empire » Emperors of, mentioned » Claudius
Famine, Examples Of
Predicting The Future
δέ
but, and, now, then, also, yet, yea, so, moreover, nevertheless, for, even, , not tr
Usage: 2184
there stood up
ἀνίστημι
Anistemi
arise, rise, rise up, rise again, raise up, stand up, raise up again,
ἐκ ἐξ
of, from, out of, by, on, with,
him, his, their, he, her, they, same, himself,
ὄνομα
Onoma
name, named, called, surname , named , not tr
Ἄγαβος
Agabos
and signified
σημαίνω
Semaino
διά
by, through, with, for, for ... sake, therefore , for this cause , because,
πνεῦμα
Spirit, Ghost, Spirit , Spirit , Spirit, Spirit , Spirit , human , spirit, spirit , spirit, spirit, ghost,
that there should
μέλλω
mello
shall, should, would, to come, will, things to come, not tr,
ἔσομαι
Esomai
shall be, will be, be, shall have, shall come to pass, shall, not tr,
μέγας
great, loud,
λιμός
famine, hunger, dearth
ἐπί
on, in, upon, unto, to,
ὅλος
Holos
all, whole, every whit, altogether, throughout
οἰκουμένη
Oikoumene
world, earth
ὅστις ἥτις ὅ6τι
hostis
which, who, whosoever, that, whatsoever , whosoever , whatsoever 9 ,
came to pass
γίνομαι
Ginomai
be, come to pass, be made, be done, come, become, God forbid , arise, have, be fulfilled, be married to, be preferred, not tr, , vr done
the days of Claudius
Κλαύδιος
Klaudios
Claudius , Claudius
CLAUDIUS CAESAR
Luke, Gospel According to
Antioch in Syria
World, The
Word Count of 38 Translations in Acts 11:28
Famine (13)
Great (12)
Stood (11)
Reign (6)
During (5)
Throughout (5)
Took (5)
Predicted (4)
Signified (4)
Inhabited (3)
Publicly (2)
Indicated (2)
Upon (2)
Dearth (2)
Predict (1)
Instructed (1)
Whom (1)
Occur (1)
Prophesied (1)
Foretold (1)
Certainly (1)
Indicate (1)
Began (1)
Signify (1)
Going (1)
Among (1)
Fulfilled (1)
Entire (1)
Acts Rank: 490
2 Readings
Developments In The Church In Antioch
27 About that time some prophets from Jerusalem came down to Antioch, 28 and one of them named Agabus got up and under the influence of the Spirit revealed the fact that there was going to be a great famine all over the world. This was the famine that occurred in the reign of Claudius. 29 The disciples determined to make up a contribution, each according to his ability, and send it to the brothers who lived in Judea,
Famine Relief
All the Earth
We spent a number of days there, and in the course of them a prophet named Agabus came down from Judea.
There he found a Jew named Aquila, a native of Pontus, who had recently come from Italy with his wife Priscilla, because Claudius had ordered all Jews to leave Rome. Paul went to see them,
And this good news of the kingdom will be preached all over the world, to testify to all the heathen, and then the end will come.
In those days an edict was issued by the Emperor Augustus that a census of the whole world should be taken.
In the fifteenth year of the reign of the Emperor Tiberius, when Pontius Pilate was governor of Judea, and Herod governor of Galilee, while his brother Philip was governor of the territory of Iturea and Trachonitis, and Lysanias was the governor of Abilene,

redpajama_set_name: RedPajamaCommonCrawl


FUJIFILM North America Corporation today unveiled the new FUJIFILM X-T3, launching the X Series mirrorless digital cameras into its fourth generation. Introducing an all-new back-illuminated 26.1MP X-Trans CMOS 4 sensor and X-Processor 4 processor, the X-T3 delivers superb image quality, dramatically improved AF performance, exceptional tracking performance of fast-moving subjects and blackout-free burst shooting. The X-T3 is also the first APS-C mirrorless camera capable of 4K/60P 10bit recording to meet the needs of professional videographers.
"We are proud to introduce the new X-T3 to market as not only the latest addition to our X Series mirrorless lineup of digital cameras, but as an introduction to fourth generation technologies that feature substantial performance enhancements over previous models, delivering high AF performance, superb color reproduction and outstanding image quality to photographers and videographers alike," said Yuji Igarashi, General Manager of the Electronic Imaging Division and Optical Devices Division of FUJIFILM North America Corporation.
Featuring the latest, fourth generation 26.1MP X-Trans CMOS 4 APS-C sensor with no optical low-pass filter, the FUJIFILM X-T3 boasts the highest performance in the history of X Series. Utilizing the unique color filter array of X-Trans CMOS sensors to control moiré and false colors, it is the first APS-C back-illuminated structure sensor with phase detection pixels distributed across the surface to improve image resolution without compromising signal to noise ratio. With the X-T3, ISO160 is now part of the standard ISO range, previously this was only available as extended ISO, perfect for use in bright scenes or when trying to shoot wide open with a fast, large-aperture lens.
The new X-T3 debuts the X-Processor 4 processor which features a Quad Core CPU to achieve a processing speed 3 times faster than current X Series models. Providing incredible AF accuracy and speed, the processor is also the first to deliver 4K/60P 10bit output, fulfilling the performance needs of professional videographers. It is also capable of implementing complex image processing tasks in an instant, such as the unique FUJIFILM Color Chrome Effect or Monochrome Adjustment function.
The new FUJIFILM X-T3 features the Color Chrome Effect, previously limited to the FUJIFILM GFX 50S medium format mirrorless digital camera, which produces enhanced color gradation in highly saturated colors such as vivid-colored flowers with shadows, a notoriously difficult subject to reproduce. With the high-speed processing power of the X-Processor 4, this effect can be applied not only to a single shot but also during continuous shooting.
With the X-T3, AF performance receives a dramatic improvement from previous X Series products, increasing the phase detection AF area to the entire frame with 2.16M phase detection pixels providing fast and accurate phase detection AF. The low-light phase detection AF limit has been increased over the X-T2 by 2 stops, from -1EV to -3EV, making it possible for photographers to accurately focus in low light conditions, perfect for night photography.
Furthermore, the X-Processor 4's high processing speed and excellent phase detection algorithm means the camera refocuses (AF) and meters (AE) 1.5 times more frequently than the X-T2, enabling accurate autofocus even when photographing subjects that change speed and move erratically across the frame, such as sports photography. The performance of Face Detection AF has also been improved. Eye Detection AF now supports the AF-C mode to maintain accurate focus tracking. Face and Eye Detection AF is also available during video recording to achieve smooth filming of subjects.
The X-T3 offers photographers incredible viewfinder performance, with the ability to now track a moving subject in the 3.69-million-dot high resolution EVF with a magnification ratio of 0.75x. Further the X-T3, allows continuous shooting of 11fps with the mechanical shutter without the optional vertical battery grip, enabling fast continuous shooting while maintaining a low weight.
Additional enhancements to the X-T3 include a display time lag of just 0.005 seconds and a EVF refresh rate of 100fps for smooth display of moving subject or when panning the camera. A new sports finder mode for enhanced capture of moving subjects is especially useful for sports and wildlife photography, and a new pre-shoot function, which allows photographers to start shooting as soon as the shutter button is half-pressed to ensure the moment is never missed.
The X-T3 is the first mirrorless digital camera capable of internal SD card 4K/60P 4:2:0 10bit recording and the first mirrorless digital camera with APS-C or larger sensor that is capable of 4K/60P 4:2:2 10bit HDMI output. Supported video formats include the widely-used H.264/MPEG-4 AVC as well as H.265/HEVC for greater data compression, which enables internal recording of 4K/60P 4:2:0 10bit at200Mbps bitrate as well as simultaneous HDMI output. The X-T3 sensor's read speed has been increased from that of the X-T2, enabling17msec reading in 4K/60P video, reducing rolling shutter distortion for smooth recording of fast-moving subjects. 10bit color depth for video boosts the amount of color information 64 times compared to 8bit, and is combined with approximately. 12 stops dynamic range to enable capture of subjects with rich gradation of color.
Also introduced in the X-T3 is a new noise reduction algorithm and 4K inter-frame noise reduction, the minimum sensitivity for shooting F-Log footage has been lowered from ISO800 to ISO640, further enhancing the camera's performance to meet the needs of videographers.
Inheriting the popular design of the X-T2, dials are positioned on the top panel, and the camera features a central viewfinder and excellent grip design for stability and comfort. The X-T3 also incorporates enhancements to its design, including a lockable EVF diopter adjustment to prevent unintended adjustments while carrying the camera, and a touchscreen panel with higher contrast, wider viewing angles and better functionality for more intuitive operation. In addition, the X-T3 offers larger top-panel dials than the X-T2, and larger rear-panel buttons and a more pronounced press function of the front and rear command dials, as found on the X-H1.
The Vertical Battery Grip VG-XT3 is designed to be dust-resistant, weather-resistant and capable of operating at temperatures as low as -10°C/14° F, and holds two additional batteries to increase the maximum number of shots to 1,100 (in normal mode). The grip features a shutter release button, focus lever, AE-L button, AF-L button, front and rear command dials, Q button and Fn button to provide the same level of excellent operability with vertical shooting as with horizontal.
The MHG-XT3 Metal Hand Grip makes it substantially more comfortable to hold the camera when it is mounted with a large-aperture lens, in order to reduce camera shake. The battery or SD cards can be replaced without having to remove the hand grip, and the base can be used as a quick release shoe when using a dovetail mount on a tripod.


text:
GVGK Tang
Exhibitionism: Rules of Gazing at Material Culture
What does it mean to exhibit material culture? What often comes to my mind is an object stuck behind a glass case or up on a wall in a cold and uninviting space, with only a small block of text offering a singular, linear account of its story. Where did this framework for history-making come from and what purpose does it serve?
Let's consider Barbara Kirshenblatt-Gimblett's chapter "Objects of Ethnography" in Exhibiting Cultures: The Poetics and Politics of Museum Display (edited by Ivan Karp and Steven Lavine). In it, the author defines an object in situ as "a part that stands in contiguous relation to an absent whole that may or may not be recreated" (388). The act of exhibition is often one of erecting artificial context around de-contextualized material. As such, one finds that museums, galleries and similar sites are simultaneously performative and voyeuristic – a space wherein a thing (or person) may be positioned, watched, known and classified in an intangible stream of consciousness curated by interpreters and fit into historical and aesthetical systems.
"Peekaboo by Winston Hacking" GIPHY. Accessed March 24, 2019. https://giphy.com/gifs/E8K43gNQBAjjG.
For What It's Worth: Defining "Fashion" History & Its Materials
"Feudal China Animation by Yang Hua Chun" GIPHY. Accessed March 6, 2019. https://giphy.com/gifs/ancient-beijing-m3SgylPA0MAlq.
Let's return to a previous post. I attempted to define "clothes," "clothing," "style," "fashion," and "costume" – differentiating between seemingly synonymous subjects for the sake of historical (or anthropological) study. Using the Oxford Dictionary and Daniel Miller's chapter "Why Clothing is Not Superficial" in Stuff, I settled on the following:
Clothes: items worn to cover the body
Clothing: clothes collectively
Style: the wearing of clothing, hair, and accessories in a manner unique to an individual body.
Fashion: a popular or the latest style of clothing, hair, accessories, or behavior; the collective following of a trend; the production and marketing of said styles and trends.
Costume: a set of clothes in a style typical of or popular in a particular country or historical period.
While Miller, an anthropologist, concerns himself with the study of clothing – its popularity and how it is worn or used – historians lay claim to the study of costume – drawn to the term for its use of periodization, then versus now. In my previous post, I argued that historians tend to study fashion. Working with extant primary sources (often what was sent to archives by wealthy donors), they focus on clothing that was popular or, rather, well-documented – typical of a small and privileged demographic (such as white, upper-class women) – rather than what was commonly worn in a certain context.
Today, I'm adding to this argument. First, one must recognize the connotations of words like "popular" (relating to the majority of a particular demographic), "latest" (new), "trending" (popular at the moment), and "typical" (commonplace, habitual, customary, or ordinary). The relationship between time and place are ever present – difficult to communicate through inexact terminology.


Quadrille is a beautiful 4-bedroom Spanish villa overlooking the Caribbean Sea in the private beach community of Silver Sands. Located approximately 45 minutes from Montego Bay the Silver Sands beach is one of the finest in Jamaica. Quadrille offers the facilities of the Silver Sands Resort with the privacy of your own vacation home. Quadrille's beach access is probably the finest in Silver Sands. The villa has been totally renovated from top to bottom including screening windows and doors for added comfort.
The villa has a large living room and dining room for comfortable relaxing and dining. There are lovely sea views from all the rooms. The spacious walk out veranda is also used for outdoor dining. A pathway down the hillside leads to a secluded gazebo half way down to the beach. The large swimming pool with pool deck (10 x 30 feet) for sunning affords views of the sea, beach and stunning Caribbean sunsets.
Quadrille has 4 bedrooms, 3 with en-suite bathrooms, the fourth has an adjoining bathroom. The Master bedroom and Bedroom #2 are on the upper level; the other two are on the lower level. Both levels have large 12 x 20 foot terraces.
-Master Bedroom Upper level, ocean (north) view. King bedroom with air conditioning, ceiling fans, and full bath and shower.
-Bedroom 2 Upper level, ocean view. Queen bedroom with air conditioning, ceiling fans, and bathroom with full bath and shower.
-Bedroom 3 Lover level. King bedroom with air conditioning and full bath.
-Bedroom 4 Lower level. King bedroom with air conditioning, and full bath. Ocean view.
Your personal villa staff will attend to your every need, and do everything to insure a most enjoyable and memorable stay at Quadrille.
Villa has recently added screens to windows and doors.


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VCCSOnline Home > Colleges > Southwest Virginia > Courses > Diesel (DSL)
Diesel (DSL) at Southwest Virginia Community College
All colleges SWCC Advanced Search >>
DSL 90 - Coordinated Internship
Supervises on-the-job training in selected business, industrial or service firms coordinated by the college.
Credit/practice ratio not to exceed 1:5 hours. May be repeated for credit. Variable hours.
DSL 95 - Topics In
DSL 98 - Seminar and Project
DSL 121 - Diesel Engines I
Studies the basic principles involved in the construction and operation of diesel engines. Examines fuel, air, cooling, and control system of various designs. Emphasizes engine overhaul and repair, including gauging proper measuring instruments and tools for these tasks. Part I of II.
DSL 122 - Diesel Engines II
Studies the basic principles involved in the construction and operation of diesel engines. Examines fuel, air, cooling, and control system of various designs. Emphasizes engine overhaul and repair, including gauging proper measuring instruments and tools for these tasks. Part II of II.
DSL 126 - Diesel Engine Reconditioning
Provides basic knowledge of the construction, design, and application of selected modern diesel engines and their components. Covers induction and exhaust systems, cooling and lubricating systems, and fuel injection and governing systems. Provides opportunity to disassemble, inspect, recondition, reassemble, and test selected engines.
Lecture 3 hours. Laboratory 6 hours. Total 9 hours per week.
DSL 131 - Diesel Fuel Systems and Tune-Up
Teaches maintenance, adjustment, testing, and general repair of the typical fuel injection components used on non- automotive diesel engines. Includes engine and fuel system tune-up procedures and troubleshooting using current diagnostic equipment.
DSL 141 - Transportation Electrical Systems I
Studies basic operational theory of electrical systems used in public transportation vehicles. Covers electrical symbols, schematics, troubleshooting procedures, as well as the function, construction, and operation of the electrical system and its components. Part I of II.
Lecture 2 hours per week.
DSL 142 - Transportation Electrical Systems II
Studies basic operational theory of electrical systems used in public transportation vehicles. Covers electrical symbols, schematics, troubleshooting procedures, as well as the function, construction, and operation of the electrical system and its components. Part II of II.
DSL 143 - Diesel Truck Electrical Systems
Studies the theory and operation of various truck and tractor electrical systems. Covers preheating, starting, generating, and lighting systems. Uses modern test equipment for measurement, adjustment, and troubleshooting.
Lecture 2 hours per week. Laboratory 4 hours. Total 6 hours per week.
DSL 152 - Diesel Power Trains, Chassis, and Suspension
Studies the chassis, suspension, steering and brake systems found on medium and heavy-duty diesel trucks. Covers construction features, operating principles and service procedures for such power train components as clutches, multi-speed transmissions, propeller shafts, and rear axles. Teaches operations of modern equipment to correct and adjust abnormalities.
DSL 161 - Air Brake Systems I
Studies the basic operational theory of pneumatic and air brake systems used in public transportation vehicles. Covers various air control valves, air and test system components, and advanced air system schematics. Part I of II.
DSL 162 - Air Brake Systems II
Studies the basic operational theory of pneumatic and air brake systems used in public transportation vehicles. Covers various air control valves, air and test system components, and advanced air system schematics. Part II of II.
DSL 181 - Diesel Mechanics I
Studies basic internal engines, including cylinder blocks, cylinder heads, crankshafts, and pistons. Studies fuel injection systems, fuel pumps, injectors and nozzles, preventive maintenance and troubleshooting. Part I of II.
Lecture 4 hours. Laboratory 6 hours. Total 10 hours per week.
DSL 182 - Diesel Mechanics II
Studies basic internal engines, including cylinder blocks, cylinder heads, crankshafts, and pistons. Studies fuel injection systems, fuel pumps, injectors and nozzles, preventive maintenance and troubleshooting. Part II of II.
DSL 190 - Coordinated Internship
DSL 195 - Topics In
DSL 199 - Supervised Study
DSL 296 - On-Site Training
Specializes in career orientation and training program without pay in selected businesses and industry, supervised and coordinated by the college.
Credit/work ratio not to exceed 1:5 hours. May be repeated for credit. Variable hours.


in legal parlance, is a leader's fag who gets up the facts of a brief, with the laws bearing on it, and arranges everything for the pleader in methodical order.
These juniors have surplus briefs handed to them by their seniors. A good fag is a good devil and is sure to get on.
The Attorney-General's devils are the Counsel of the Treasury, who not unfrequently get promoted to the bench.
"I, Aldo Manuzio, printer to the Doge, have this day made public exposure of the printer's devil. All who think he is not flesh and blood may come and pinch him."—Proclamation of Aldo Manuzio, 1490.
Rose: Orlando Furioso, iii. 32.


[bug#33186] [PATCH 4/7] gnu: glibc: Export supported locales.
Subject: [bug#33186] [PATCH 4/7] gnu: glibc: Export supported locales.
> locales of the glibc.
> * gnu/packages/patches/glibc-supported-locales.patch: New file.
Note that this is for 'core-updates-next'.
> +This patch is taken from debian's glibc package (generate-supported.mk).
Please add a line saying what it does.
Prev by Date: [bug#33186] [PATCH 3/7] services: kmscon: Add an auto-login option.
Next by Date: [bug#33186] [PATCH 5/7] gnu: terminals: Change libtsm and kmscon	repositories.
Previous by thread: [bug#33186] [PATCH 3/7] services: kmscon: Add an auto-login option.
Next by thread: [bug#33186] [PATCH 4/7] gnu: glibc: Export supported locales.


text:
Huimilpan är en kommun i Mexiko.   Den ligger i delstaten Querétaro Arteaga, i den centrala delen av landet,  km nordväst om huvudstaden Mexico City. Antalet invånare är . Arean är  kvadratkilometer.

Terrängen i Huimilpan är huvudsakligen kuperad, men västerut är den platt.

Följande samhällen finns i Huimilpan:

 Huimilpan
 El Vegil
 Las Taponas
 Los Cues
 Apapátaro
 San José Tepuzas
 San Antonio la Galera
 Cumbres del Cimatario
 Guadalupe Primero
 Buenavista
 San Pedrito
 Puerta del Tepozán
 El Sauz
 Paniagua
 La Haciendita
 Capula
 Los Bordos
 El Peral
 La Joya
 Nevería II
 El Salto
 La Nueva Joya
 El Rincón
 El Garruñal
 Pío XII
 Las Monjas
 Nevería Sector Norte
 San Antonio del Puente
 El Centenario
 Guadalupe Segundo Fracción Primera
 La Presita

I övrigt finns följande i Huimilpan:

 Kullar:
 Cerro El Aserrin (en kulle)
 Cerro Grande (en kulle)
 Cerro La Beata (en kulle)
 Cerro La Cruz (en kulle)
 Cerro La Víbora (en kulle)
 Cerro Las Monjas (en kulle)

 Berg:
 Cerro Bravo (ett berg)
 Cerro Capula (ett berg)
 Cerro Cimatario (ett berg)
 Cerro El Aguila (ett berg)
 Cerro La Cruz (ett berg)
 Cerro La Cruz (ett berg)
 Cerro Tres (ett berg)

Ett tropiskt höglandsklimat råder i trakten. Årsmedeltemperaturen i trakten är  °C. Den varmaste månaden är april, då medeltemperaturen är  °C, och den kallaste är september, med  °C. Genomsnittlig årsnederbörd är  millimeter. Den regnigaste månaden är juli, med i genomsnitt  mm nederbörd, och den torraste är januari, med  mm nederbörd.

Kommentarer

Källor

Indelningar i Querétaro Arteaga

redpajama_set_name: RedPajamaWikipedia


My father has found an old choir picture from 1966 or 1967. The East Huntspill Choir children all attended the East Huntspill County Primary School when the formidable Miss Wesley was the headmistress, my mother Joan was the secretary and the dinner money lady!
Penny Knolwes I believe is still living in Nut Tree Close and her father used to help rehearse the choir for some time with Sue Elsworth accompanying us on the organ.
They were good days when we were all so close. The Vicar used to have summer fetes in his garden at The Vicarage in Withy Road. The children would be in fancy dress andthere would be games to play and races to enter. The Local Mayor & Mayoress would cut the ribbon and I remember being chosen one year to present The Mayoress with a boquet of flowers.
There would be a marquee with flowers, fruit & vegetabales on display. The ladies of the village would enter beautiful cakes, jams & chutneys, there was always fierce competition over the produce on display!
Tombolas, coconut shy, raffles, bottled stalls, knitted toys & homemade sweets. I will never forget Miss Wesley's Coconut Ice!!
Every year there would be a Sunday School Outing and I remember a coach full of villagers going to Butlins at Minehead!
In the Church hall there was always regular Jumble Sales, 50/50 Auctions, Beetle Drives, WI Meetings and Sunday School.
Harvest time was always a joyous occasion with The Church looking beautiful with local produce displayed around the alter, I remember the sun pouring through the Church Stain Glass Window lighting up the flower displays.
The Harvest Home was a major event with a parade starting from the orchard past the old Post Office at the top end of Bason Bridge going down to the Church, the fair site was in the field opposite in those days.
My Mother and Mrs Nancarrow with a few other ladies were in charge of the Church Flowers. The ladies also got involved in making the cross stitch kneelers that adorned the church.
My mother & I used to cycle around the village delivering the Parish Magazine. My first ever job was keeping the bus shelters clean. I would cycyle from one end of the village to the other with a broom and a bag for the litter in my shopping basket!
Anyway, thankyou for bringing back some many memories and I wish you luck in your continued research.
We lived in New Road next to what was the Lamb Inn, as a child I always enjoyed playing in the skittle alley!


Visit Our Partner, Carry Out Supplies, For your wholesale solution for custom and generic takeout supplies!
All the wholesale supplies you need for starting your frozen yogurt shop are right at your finger tips, through our partnering Carry Out Supplies company. Frozen yogurt cups, lids and spoons are available in a vast array of colors, sizes, and customized printing with your logo and more.
Plain white sample cups will make for a very happy customer base! Of course most every cup is going to need a lid which is offered in both flat and dome styles for all sizes, with the exception of the 0.5 ounce sample size. A slightly non-traditional yet exciting material option is the 100% compostable paper cups for delicious sweet treats along with suitable lids. These are obtainable in three sizes, however, the biodegradable lid is only offered in the flat lid style.
Looking to add a little spark to your business with paper cups that can garner more attention for your store? Have the cups custom printed with your brand name, logo, or design. All of the paper cups can be custom printed at no extra charge to you, using food grade soy ink.
Select from the wide assortment of plastic spoons. With your choice of 16 color options, customers can finish off their dessert in style. Come see what Carry Out Supplies has to offer you with their frozen yogurt supplies!


text:
Home / Concepts / Multilocus Sequence Typing
Multilocus Sequence Typing
More general concepts than this:
Chapter [63] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Chapter
Article [14] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Article
MicrobiologySpectrumArticle [7] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/MicrobiologySpectrumArticle
Backmatter [3] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Backmatter
Frontmatter [1] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/Frontmatter
MagazineArticle [1] http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://pub2web.metastore.ingenta.com/ns/MagazineArticle
2016 [6] http://pub2web.metastore.ingenta.com/ns/yearOfPublication 2016
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Impact of Genetic Diversity on the Biology of Mycobacterium tuberculosis Complex Strains
Authors: Stefan Niemann, Matthias Merker, Thomas Kohl, Philip Supply
Citation: Niemann S, Merker M, Kohl T, Supply P. 2016. Impact of genetic diversity on the biology of mycobacterium tuberculosis complex strains. 4(6): doi:10.1128/microbiolspec.TBTB2-0022-2016
DOI 10.1128/microbiolspec.TBTB2-0022-2016
Tuberculosis (TB) remains the most deadly bacterial infectious disease worldwide. Its treatment and control are threatened by increasing numbers of multidrug-resistant (MDR) or nearly untreatable extensively drug-resistant (XDR) strains. New concepts are therefore urgently needed to understand the factors driving the TB epidemics and the spread of different strain populations, especially in association with drug resistance. Classical genotyping and, more recently, whole-genome sequencing (WGS) revealed that the world population of tubercle bacilli is more diverse than previously thought. Several major phylogenetic lineages can be distinguished, which are associated with their sympatric host population. Distinct clonal (sub)populations can even coexist within infected patients. WGS is now used as the ultimate approach for differentiating clinical isolates and for linking phenotypic to genomic variation from lineage to strain levels. Multiple lines of evidence indicate that the genetic diversity of TB strains translates into pathobiological consequences, and key molecular mechanisms probably involved in differential pathoadaptation of some main lineages have recently been identified. Evidence also accumulates on molecular mechanisms putatively fostering the emergence and rapid expansion of particular MDR and XDR strain groups in some world regions. However, further integrative studies will be needed for complete elucidation of the mechanisms that allow the pathogen to infect its host, acquire multidrug resistance, and transmit so efficiently. Such knowledge will be key for the development of the most effective new diagnostics, drugs, and vaccination strategies.
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Reducing Foodborne Pathogen Persistence and Transmission in Animal Production Environments: Challenges and Opportunities
Authors: Elaine D. Berry, James E. Wells
Citation: Berry E, Wells J. 2016. Reducing foodborne pathogen persistence and transmission in animal production environments: challenges and opportunities. 4(4): doi:10.1128/microbiolspec.PFS-0006-2014
DOI 10.1128/microbiolspec.PFS-0006-2014
Preharvest strategies to reduce zoonotic pathogens in food animals are important components of the farm-to-table food safety continuum. The problem is complex; there are multiple pathogens of concern, multiple animal species under different production and management systems, and a variety of sources of pathogens, including other livestock and domestic animals, wild animals and birds, insects, water, and feed. Preharvest food safety research has identified a number of intervention strategies, including probiotics, direct-fed microbials, competitive exclusion cultures, vaccines, and bacteriophages, in addition to factors that can impact pathogens on-farm, such as seasonality, production systems, diet, and dietary additives. Moreover, this work has revealed both challenges and opportunities for reducing pathogens in food animals. Animals that shed high levels of pathogens and predominant pathogen strains that exhibit long-term persistence appear to play significant roles in maintaining the prevalence of pathogens in animals and their production environment. Continued investigation and advancements in sequencing and other technologies are expected to reveal the mechanisms that result in super-shedding and persistence, in addition to increasing the prospects for selection of pathogen-resistant food animals and understanding of the microbial ecology of the gastrointestinal tract with regard to zoonotic pathogen colonization. It is likely that this continued research will reveal other challenges, which may further indicate potential targets or critical control points for pathogen reduction in livestock. Additional benefits of the preharvest reduction of pathogens in food animals are the reduction of produce, water, and environmental contamination, and thereby lower risk for human illnesses linked to these sources.
Comparative Microbial Genomics and Forensics
Author: Steven E. Massey
Citation: Massey S. 2016. Comparative microbial genomics and forensics. 4(4): doi:10.1128/microbiolspec.EMF-0001-2013
DOI 10.1128/microbiolspec.EMF-0001-2013
Forensic science concerns the application of scientific techniques to questions of a legal nature and may also be used to address questions of historical importance. Forensic techniques are often used in legal cases that involve crimes against persons or property, and they increasingly may involve cases of bioterrorism, crimes against nature, medical negligence, or tracing the origin of food- and crop-borne disease. Given the rapid advance of genome sequencing and comparative genomics techniques, we ask how these might be used to address cases of a forensic nature, focusing on the use of microbial genome sequence analysis. Such analyses rely on the increasingly large numbers of microbial genomes present in public databases, the ability of individual investigators to rapidly sequence whole microbial genomes, and an increasing depth of understanding of their evolution and function. Suggestions are made as to how comparative microbial genomics might be applied forensically and may represent possibilities for the future development of forensic techniques. A particular emphasis is on the nascent field of genomic epidemiology, which utilizes rapid whole-genome sequencing to identify the source and spread of infectious outbreaks. Also discussed is the application of comparative microbial genomics to the study of historical epidemics and deaths and how the approaches developed may also be applicable to more recent and actionable cases.
Whole-Genome Sequencing Is Taking over Foodborne Disease Surveillance
Authors: Heather A. Carleton, and Peter Gerner-Smidt
Source: , July 2016, 11:311-317; doi:10.1128/microbe.11.311.1
Publication Date : July 2016 DOI 10.1128/microbe.11.311.1
About 1 in 6 people are sickened with foodborne diseases each year, and for the most part those illness bouts are a nuisance and self-limiting. However, for some vulnerable populations, severe foodborne illnesses can require hospital care and may even lead to death. Once an individual becomes sick enough to visit a physician, he or she typically collects a stool sample to send to a clinical microbiology laboratory for testing and diagnosis. If the clinical laboratory identifies an enteric pathogen, the physician is notified.
Mobilization of Carbapenemase-Mediated Resistance in Enterobacteriaceae
Author: Amy Mathers
Citation: Mathers A. 2016. Mobilization of carbapenemase-mediated resistance in enterobacteriaceae. 4(3): doi:10.1128/microbiolspec.EI10-0010-2015
DOI 10.1128/microbiolspec.EI10-0010-2015
There has been a dramatic increase in the last decade in the number of carbapenem-resistant Enterobacteriaceae, often leaving patients and their providers with few treatment options and resultant poor outcomes when an infection develops. The majority of the carbapenem resistance is mediated by bacterial acquisition of one of three carbapenemases (Klebsiella pneumoniae carbapenemase [KPC], oxacillinase-48-like [OXA-48], and the New Delhi metallo-β-lactamase [NDM]). Each of these enzymes has a unique global epidemiology and microbiology. The genes which encode the most globally widespread carbapenemases are typically carried on mobile pieces of DNA which can be freely exchanged between bacterial strains and species via horizontal gene transfer. Unfortunately, most of the antimicrobial surveillance systems target specific strains or species and therefore are not well equipped for examining genes of drug resistance. Examination of not only the carbapenemase gene itself but also the genetic context which can predispose a gene to mobilize within a diversity of species and environments will likely be central to understanding the factors contributing to the global dissemination of carbapenem resistance. Using the three most prevalent carbapenemase genes as examples, this chapter highlights the potential impact the associated genetic mobile elements have on the epidemiology and microbiology for each carbapenemase. Understanding how a carbapenemase gene mobilizes through a bacterial population will be critical for detection methods and ultimately inform infection control practices. Understanding gene mobilization and tracking will require novel approaches to surveillance, which will be required to slow the spread of this emerging resistance.
Bacterial Prostatitis: Bacterial Virulence, Clinical Outcomes, and New Directions
Authors: John N. Krieger, Praveen Thumbikat
Citation: Krieger J, Thumbikat P. 2016. Bacterial prostatitis: bacterial virulence, clinical outcomes, and new directions. 4(1): doi:10.1128/microbiolspec.UTI-0004-2012
DOI 10.1128/microbiolspec.UTI-0004-2012
Four prostatitis syndromes are recognized clinically: acute bacterial prostatitis, chronic bacterial prostatitis, chronic prostatitis/chronic pelvic pain syndrome, and asymptomatic prostatitis. Because Escherichia coli represents the most common cause of bacterial prostatitis, we investigated the importance of bacterial virulence factors and antimicrobial resistance in E. coli strains causing prostatitis and the potential association of these characteristics with clinical outcomes. A structured literature review revealed that we have limited understanding of the virulence-associated characteristics of E. coli causing acute prostatitis. Therefore, we completed a comprehensive microbiological and molecular investigation of a unique strain collection isolated from healthy young men. We also considered new data from an animal model system suggesting certain E. coli might prove important in the etiology of chronic prostatitis/chronic pelvic pain syndrome. Our human data suggest that E. coli needs multiple pathogenicity-associated traits to overcome anatomic and immune responses in healthy young men without urological risk factors. The phylogenetic background and accumulation of an exceptional repertoire of extraintestinal pathogenic virulence-associated genes indicate that these E. coli strains belong to a highly virulent subset of uropathogenic variants. In contrast, antibiotic resistance confers little added advantage to E. coli strains in these healthy outpatients. Our animal model data also suggest that certain pathogenic E. coli may be important in the etiology of chronic prostatitis/chronic pelvic pain syndrome through mechanisms that are dependent on the host genetic background and the virulence of the bacterial strain.
The "Cryptic" Escherichia
Author: Seth T. Walk
Citation: Walk S. 2015. The "Cryptic" Escherichia, EcoSal Plus 2015; doi:10.1128/ecosalplus.ESP-0002-2015
DOI 10.1128/ecosalplus.ESP-0002-2015
In 2009, five monophyletic Escherichia clades were described and referred to as "cryptic" based on the inability to distinguish them from representative E. coli isolates using diagnostic biochemical reactions. Since this original publication, a number of studies have explored the genomic, transcriptomic, and phenotypic diversity of cryptic clade isolates to better understand their phylogenetic, physiological, and ecological distinctiveness with respect to previously named Escherichia species. This chapter reviews the original discovery of the cryptic clades, discusses available evidence that some are environmentally adapted, and evaluates current support for taxonomic designations of these microorganisms. The importance of these clades to clinical research, epidemiology, population genetics, and microbial speciation is also discussed.
Do You Kiss Your Mother with That Mouth? An Authentic Large-Scale Undergraduate Research Experience in Mapping the Human Oral Microbiome †
Authors: Jack T. H. Wang*, Joshua N. Daly, Dana L. Willner, Jayee Patil, Roy A. Hall, Mark A. Schembri, Gene W. Tyson, Philip Hugenholtz
Citation: Wang J, Daly J, Willner D, Patil J, Hall R, Schembri M, Tyson G, Hugenholtz P. 2015. Do you kiss your mother with that mouth? an authentic large-scale undergraduate research experience in mapping the human oral microbiome † . 16(1):50-60 doi:10.1128/jmbe.v16i1.816
DOI 10.1128/jmbe.v16i1.816
Clinical microbiology testing is crucial for the diagnosis and treatment of community and hospital-acquired infections. Laboratory scientists need to utilize technical and problem-solving skills to select from a wide array of microbial identification techniques. The inquiry-driven laboratory training required to prepare microbiology graduates for this professional environment can be difficult to replicate within undergraduate curricula, especially in courses that accommodate large student cohorts. We aimed to improve undergraduate scientific training by engaging hundreds of introductory microbiology students in an Authentic Large-Scale Undergraduate Research Experience (ALURE). The ALURE aimed to characterize the microorganisms that reside in the healthy human oral cavity—the oral microbiome—by analyzing hundreds of samples obtained from student volunteers within the course. Students were able to choose from selective and differential culture media, Gram-staining, microscopy, as well as polymerase chain reaction (PCR) and 16S rRNA gene sequencing techniques, in order to collect, analyze, and interpret novel data to determine the collective oral microbiome of the student cohort. Pre- and postsurvey analysis of student learning gains across two iterations of the course (2012–2013) revealed significantly higher student confidence in laboratory skills following the completion of the ALURE (p < 0.05 using the Mann-Whitney U-test). Learning objectives on effective scientific communication were also met through effective student performance in laboratory reports describing the research outcomes of the project. The integration of undergraduate research in clinical microbiology has the capacity to deliver authentic research experiences and improve scientific training for large cohorts of undergraduate students.
Shiga Toxin-Producing Escherichia coli (STEC) in Fresh Produce—A Food Safety Dilemma
Author: Peter Feng
Citation: Feng P. 2014. Shiga toxin-producing escherichia coli (stec) in fresh produce—a food safety dilemma. 2(4): doi:10.1128/microbiolspec.EHEC-0010-2013
DOI 10.1128/microbiolspec.EHEC-0010-2013
Produce contains high levels of mixed microflora, including coliforms and Escherichia coli, but occasionally pathogens may also be present. Enterotoxigenic E. coli and Shigatoxin-producing E. coli (STEC) have been isolated from various produce types, especially spinach. The presence of STEC in produce is easily detected by PCR for the Shiga toxin (Stx) gene, stx, but this is insufficient for risk analysis. STEC comprises hundreds of serotypes that include known pathogenic serotypes and strains that do not appear to cause severe illness. Moreover, Stx without a binding factor like intimin (encoded by eae) is deemed to be insufficient to cause severe disease. Hence, risk analyses require testing for other virulence or serotype-specific genes. Multiplex PCR enables simultaneous testing of many targets, but, in a mixed flora sample, not all targets detected may be coming from the same cell. The need to isolate and confirm STEC in produce is critical, but it is time- and labor-intensive due to the complexity of the group. Studies showed that only a handful of STEC strains in produce have eae, and most belonged to recognized pathogenic serotypes so are of definite health risks. Several eae-negative strains belonged to serotypes O113:H21 and O91:H21 that historically have caused severe illness and may also be of concern. Most of the other STEC strains in produce, however, are only partially serotyped or are unremarkable serotypes carrying putative virulence factors, whose role in pathogenesis is uncertain, thus making it difficult to assess the health risks of these STEC strains.
Wooden Tools: Reservoirs of Microbial Biodiversity in Traditional Cheesemaking
Authors: Sylvie Lortal, Giuseppe Licitra, Florence Valence
Citation: Lortal S, Licitra G, Valence F. 2014. Wooden tools: reservoirs of microbial biodiversity in traditional cheesemaking. 2(1): doi:10.1128/microbiolspec.CM-0008-2012
DOI 10.1128/microbiolspec.CM-0008-2012
Today, wooden shelves are used for the ripening of about 500,000 tons of cheese per year in Europe, including about 350,000 tons in France, such as most of the famous cheeses with the protected designation of origin (PDO), e.g., Comté, Reblochon, Beaufort, Munster, Cantal, and Roquefort. For some PDO cheeses, the use of wooden tools is mandatory. Many cheesemakers believe that wooden tools improve the organoleptic and typical characteristics of their final products. Wood is a natural and sustainable material which has been used for centuries in traditional cheese production in a wide variety of forms (vats, shelves, and packaging). Wood is important in the cheesemaking process, interacting with the milk in vats or with the cheeses placed on shelves for ripening. Wood is viable due to its ability to exchange water but, above all, because it is covered by a rich microbial biofilm. As wood is porous and difficult to clean, the European Commission regularly highlights the question of its safety when in contact with food and calls for deeper scientific investigation. In this review, knowledge about the multiple technological roles of wood in dairy technology is discussed. The crucial role of wood as a reservoir of microbial biodiversity for traditional cheeses is reviewed, along with results of safety assessments. As a conclusion, the numerous questions remaining about this natural inoculating system are discussed.
Source: Cheese and Microbes , pp 167-176
For centuries, farmers and cheesemakers have used wooden tools to collect and transform milk (vats, spoons, molds, cream separators, hoops for Swiss cheese, shelves for ripening, and boxes for direct packaging such as for Vacherin Mont-d'Or) ( Fig. 1 ). Unfortunately, as wood is a biodegradable material (compared to ceramic, for example), there are very few historical artifacts documenting proof of its use in the past, in contrast to objects such as wine barrels, which are often represented on frescoes and in paintings.
Invasive Salmonellosis in Humans
Authors: Gemma C. Langridge, John Wain, and Satheesh Nair
Citation: Langridge G, Wain J, Nair S. 2012. Invasive Salmonellosis in Humans, EcoSal Plus 2012; doi:10.1128/ecosalplus.8.6.2.2
DOI 10.1128/ecosalplus.8.6.2.2
Human salmonellosis is generally associated with Salmonella enterica from subspecies enterica (subspecies I). Acute infections can present in one of four ways: enteric fever, gastroenteritis, bacteremia, or extraintestinal focal infection. As with other infectious diseases, the course and outcome of the infection depend on a variety of factors, including the infecting organism, the inoculating dose, and the immune status and genetic background of the host. For serovarsTyphi and Paratyphi A there is a clear association between the genetic background of the serovar and systemic infection in humans. For serovars Paratyphi B and Paratyphi C, a good clinical description of the host and detailed population genetics of the pathogen are necessary before more detailed genetic studies of novel virulence factors,or host factors,can be initiated. For the nontyphoidalserovars (NTS) the situation is less clear. Serovars Typhimurium and Enteritidis are the most common within the food chain, and so the large number of invasive infections associated with these serovars is most likely due to exposure rather than to increased virulence of the pathogen. In Africa, however, a closely related group of strains of serovar Typhimurium, associated with HIV infection, may have become host adapted tohumans, suggesting that not all isolates called "Typhimurium" should be considered as a single group. Here we review current knowledge of the salmonellae for which invasive disease in humans is an important aspect of their population biology.
Detection and Clinical Significance of Variability among Candida Isolates
Author: Lois L. Hoyer
Source: Candida and Candidiasis, Second Edition , pp 91-99
Strain variability is a central topic in the discussion of Candida biology. Most of the Candida isolates that are studied are derived from clinical specimens. Therefore, a discussion of variability among Candida isolates within a particular species starts with decisions that are made in the diagnostic microbiology laboratory. Studies of Candida genetic variability may also be conducted from the perspective of assessing genomic rearrangements. The nature of genomic rearrangements in the diploid Candida albicans was understood more clearly from construction of a physical map of its eight pairs of chromosomes. DNA fingerprinting probe sequences useful in phylogenetic and epidemiological studies are derived from the major repeat sequence (MRS). The MRS is also present in the closely related Candida dubliniensis. Events such as mutation and mitotic recombination also can contribute to variability among C. albicans strains. One study examined whether the local wildlife population was responsible for maintaining a reservoir of C. albicans isolates specific to a defined geographic area in the midwestern United States. The work was expanded to include collection of C. albicans isolates from domestic animals. Results showed that there is a significant difference in the clade distribution of isolates from humans and wildlife, demonstrating population isolation between the groups. The work demonstrates the impressive display of genetic variability that C. albicans can develop when challenged with exposure to antifungal drugs. A given isolate of C. albicans can undergo an impressive range of genetic changes at the level of point mutation to alterations in whole chromosomes.
Genome Plasticity in Candida albicans
Authors: Claude Pujol, David R. Soll
Source: Genome Plasticity and Infectious Diseases , pp 303-325
This chapter describes the different mechanisms of genome plasticity in Candida albicans and their impact on phenotypic plasticity, with an emphasis on recent advances in antifungal drug resistance. The average divergence between C. albicans genetic groups is approximately 2 million years. As a consequence, the recombination and genetic exchanges are most likely due to ancient mating events in C. albicans and not due to recent mating events. The requirement of sex to repair DNA damage may be moot in a diploid because sequences on homologous chromosomes can be used as templates to repair DNA breaks by an effective homologous recombination mechanism. The chapter gives a brief overview of some of the hypotheses that may particularly apply to C. albicans. A source of genome plasticity associated with recombination at MRS loci is chromosome translocation. The possibility exists that recombinations at the MRS can alter its structure and affect filamentation. In conclusion, the development of resistance to fluconazole can involve mutations at TAC1 and ERG11, as well as several genome plasticity events leading to loss of eterozygosity (LOH) and aneuploidy that affect these two genes as well as additional genes on chromosome 5.
Selfish Elements and Self-Defense in the Enterococci
Authors: Kelli L. Palmer, Michael S. Gilmore
This chapter reviews the genome plasticity that has led to distinct subpopulations of enterococci, as well as the evidence for loss of genome defenses as precipitating events in their emergence. It presents evidence as it currently exists describing the role of genome plasticity in the virulence and persistence of hospital-adapted, multidrug-resistant enterococcal lineages. It also highlights the mechanistic and comparatively unappreciated role that loss of endogenous genome defenses, such as restriction modification and clustered, regularly interspaced, short palindromic repeats (CRISPR) systems, may have played in the emergence of these lineages. To the authors knowledge, the extent to which insertion sequence (IS) element inactivation of chromosomal genes contributes to the success of enterococci as pathogens has not been explored. However, there is evidence that intragenomic recombination at IS elements has contributed substantially to genome plasticity of the enterococci. The chapter focuses on genome defense mechanisms in the enterococci, including restriction-modification and CRISPR. Much work remains to be done before understanding whether and how genome defense mechanisms influence enterococcal ecology and evolution.
Deep and Wide: Comparative Genomics of Chlamydia
Authors: Garry S. A. Myers, Jonathan Crabtree, Heather Huot Creasy
Source: Intracellular Pathogens I: Chlamydiales , pp 27-50
This chapter focuses on comparative genomics of Chlamydia species, which are well-known human and animal pathogens that constitute the family Chlamydiaceae. There were 33 chlamydial genome sequences produced by whole-genome shotgun (WGS) sequencing that are publicly available as complete or draft genomes in GenBank. The Chlamydiaceae pangenome was first examined by a BLAST score ratio (BSR) analysis using a single representative of each of the eight sequenced species. The secreted effectors identified in C. trachomatis are more divergent in other species than are the other selected virulence factors. One of the most significant features of the chlamydial plasticity zone (PZ) revealed by comparative analysis is the heterogeneity of the chlamydial cytotoxin (tox) across the Chlamydiaceae. Nevertheless, the tox variation identified here is a prime example of the utility of comparative genomics for identifying novel interspecies genetic variation for further exploration. The chapter focuses further attention on the processes of gene decay and loss that are shaping the Chlamydiaceae. The symptoms of human disease are variable, ranging from no clinical signs at all to severe systemic disease. C. trachomatis is represented by 20 of the 33 genomes currently available, as befitting the most recognizable pathogen of the family Chlamydiaceae, responsible for significant sexually transmitted disease morbidity and infectious blindness worldwide.
Fusarium and Other Opportunistic Hyaline Fungi
Authors: Deanna A. Sutton, Mary E. Brandt
Source: Manual of Clinical Microbiology, 10th Edition , pp 1853-1879
The opportunistic hyaline or lightly colored moulds constitute a phylogenetically diverse group of common to rare anamorphic and teleomorphic fungi that typically occur as saprobes in soil, in air, or on plant litter or as facultative plant pathogens. Some may be recovered from specimens without having any clinical significance. While several of the genera treated in this chapter include species having either lightly colored or dark (melanized) conidia, the emphasis is on those fungi that grow in tissue in the form of hyaline or lightly colored, septate hyphal elements. The term fusariosis is used to define infections caused by species of Fusarium, but the practice of coining disease names based on the genus of fungus involved is disadvantageous for infections caused by uncommon or rare fungal pathogens. In the chapter, the name of the teleomorph is used for species that are identified mainly by their sexual structures. Most of the pathogenic moulds considered in the chapter are classified in the form-class Hyphomycetes (genera which bear their conidia free). The conidiogenous cell produces a single conidium or multiple conidia. The number of hyaline fungal species that have been reported to cause opportunistic infection in humans and animals is increasing. The chapter also describes the salient colonial and microscopic features of the medically important species in the genus Fusarium and other selected currently recognized hyaline opportunists.
Enterococcus
Authors: Lúcia Martins Teixeira, Maria da Glória Siqueria Carvalho, Patricia Lynn Shewmaker, Richard R. Facklam
Source: Manual of Clinical Microbiology, 10th Edition , pp 350-364
Current criteria for inclusion in the genus Enterococcus and for the description of new enterococcal species encompass a polyphasic approach resulting from a combination of different molecular techniques (frequently involving DNA-DNA reassociation experiments, 16S rRNA gene sequencing, and whole-cell protein profiling analysis) and phenotypic tests. Several other molecular methods, mostly nucleic acid-based assays, have been used as additional tools to assess the phylogenetic relationships among enterococcal species and to formulate the description of new species, but their use is still limited. Positive catalase testing has also been reported for strains of Enterococcus haemoperoxidus and Enterococcus silesiacus when cultivated on blood-containing agar media. Phenotypic characteristics are used for the identification of Enterococcus species and some physiologically related species of other gram-positive cocci. Serologic tests for detecting antibody responses to different enterococcal antigens have been proposed. In addition to the intrinsic resistance traits, enterococci have acquired different genetic determinants that confer resistance to several classes of antimicrobial agents, including chloramphenicol, tetracyclines, macrolides, lincosamides and streptogramins, aminoglycosides, β-lactams, glycopeptides, quinolones, and even some of the more recently available drugs, such as linezolid, daptomycin, and quinupristin-dalfopristin. Antimicrobial resistance can be classified as either intrinsic or acquired. Molecular methods have been used to detect specific antimicrobial resistance genes and have substantially contributed to the understanding of the spread of acquired resistance among enterococci, especially resistance to vancomycin. However, because of their high specificity, molecular methods do not detect antimicrobial resistance due to mechanisms not targeted by the testing, including emerging resistance mechanisms.
Systems for Detection and Identification of Bacteria and Yeasts *
Authors: Cathy A. Petti, Melvin P. Weinstein, Karen C. Carroll
Source: Manual of Clinical Microbiology, 10th Edition , pp 15-26
Traditionally, the detection and identification of bacteria were based on conventional tube-based biochemical reactions, and their results were compared to historical charts of expected biochemical reactions. Automation in microbiology first occurred in the early 1970s with the introduction of semiautomated blood culture instruments, followed by instrumented systems for identification and susceptibility testing of bacteria. This chapter reviews the systems used for the detection of bacteria and yeasts from blood and provides an overview of technologies for microorganism identification. The volume of blood obtained for culture is one of the most important variables in the detection of bloodstream infections (BSIs). The chapter describes the identification of microorganisms by nonphenotypic methods from instrument-flagged blood culture bottles, and from pure culture. The approximate turnaround time of the fluorescence in situ hybridization (FISH) procedure is 2.5 to 3 h (without batch testing), compared with >18 to 24 h for identification of bacteria and yeasts by conventional methods. The selection of DNA targets to identify bacteria and fungi relies on the concept that some genes have conserved segments flanked by variable regions. The technology is based on analyzing the protein composition of a bacterial cell, with ribosomal proteins comprising most bacterial proteins being detected. Another technique to characterize bacteria is the application of electrospray ionization-mass spectrometry to analyze products of multilocus, broad-range PCR (PCR/ESI-MS).
Vibrio and Related Organisms *
Authors: Sharon L. Abbott, J. Michael Janda, J. J. Farmer
This chapter focuses on the various Vibrionaceae species that cause disease in humans. Vibrio is the type genus for the family, and Vibrio cholerae, the causative agent of pandemic cholera, is the type species. The genera covered in the chapter are primarily isolated from marine environments. Recent studies have shown that in mixed populations of nonculturable and culturable cells of V. cholerae, the latter appear to be the main contributors to human infections. The majority of persons ingesting toxigenic V. cholerae O1 have asymptomatic infections. Most of the advantages of PCR-based assays over culture methods apply to vibrios and include the ability to freeze stools for epidemiological studies for delayed testing. Molecular identification of vibrios is commonplace in surveys and in research studies. However, it is not commonly employed in clinical laboratories for routine identification because vibrios are relatively rare pathogens in noncoastal areas or regions where cholera is not endemic. The clinical significance of Vibrio strains in other specimens, particularly stool, may be more difficult to determine and requires prompt consultation with the attending physician to better understand the clinical context. Vibrionaceae isolates should also be submitted to public health laboratories, as they are monitored under the CDC's International Emerging Infections Program and Vibrio Surveillance System; they may also be needed for confirmation and toxin testing. Misidentification of Vibrio species and their relatives can be a problem in the literature unless investigators used methods that are very sensitive in differentiating all of the species in the family Vibrionaceae.


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The host in blue compact galaxies.
Yanling Wu, V. Charmandaris, Lei Hao, et al.
L. M. Cairós, N. Caon, B. García Lorenzo, et al.
Armando Gil de Paz, Samuel Boissier, Barry F. Madore, et al.
Kevin Schawinski, Chris Lintott, Daniel Thomas, et al.
L. M. Cairós, N. Caon, C. Zurita, et al.
Luz M. Cairos, Nicola Caon, Polychronis Papaderos, et al.
A. V. Ugryumov, D. Engels, S. A. Pustilnik, et al.
N. G. Guseva, Y. I. Izotov, P. Papaderos, et al.
The faint outskirts of the blue compact galaxy Haro 11: is there a red excess?


The documentary film Re:Generation teams up dance music DJs with musicians in traditional genres – classical, rock, R&B, jazz, and country – to combine the best of each. We noticed it on Hulu the other night and idly pressed play, not expecting much. Boy, were we wrong.
Re:Generation grabbed us and didn't let go. It's one of the best "behind the scenes" depictions I've ever seen of the creative process of composing music. The camera gets in close and follows every step of the way, putting you inside the artists heads' as they struggle to communicate, build rapport, and ultimately write songs together.
The others didn't start off much better. Pretty Lights ran into trouble when he tried to direct legendary bluegrass singer Ralph Stanley, but when he stepped back and followed Stanley's lead in the studio, the vocals on their track Wayfaring Stranger turned out evocative and otherworldly.
The odd couples didn't just contribute comic relief and happy endings. When similar people collaborate, they quickly draw on a reservoir of shared language, experience, and assumptions. Most of their communication is implicit, automatic.
The musicians in Re:Generation started out with none of that. Their surface differences hid their common musicality. They had to talk out loud, making music awkwardly as they gradually, painfully came to understand each other. The shortcuts weren't available, so they had to dig deeper and find simpler, more primitive ways to bond over the rhythm, harmony, melody, and tone of music itself.
It may have been slower and less efficient for them, but it was gold for us. We saw their process exposed and laid bare like never before. Seeing how the sausage is made may be no fun when it's sausage, but when it's music, it's wondrous.
Best of all, the soundtrack is free! It's great, and incredibly diverse, as you'd expect. Watch the movie and then listen to it. If you have any interest in music whatsoever, it's worth it. And if you don't, for the love of god, find someone to melt your heart of stone!


Overnight ratings report for the DC's Legends of Tomorrow episode "Progeny"
Despite competition from the American Idol finale, Legends of Tomorrow pretty much maintained its initial overnight ratings numbers from last week.
"Progeny" had a 0.7/3 in the Ages 18-49 demo and 2.03 million viewers. The numbers could adjust as apparently the show was pre-empted in Chicago.


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Pro-life group sues school for mandating 'trigger warnings'
Autumn Johnson | Contributing Writer, Media Research Center
Friday, December 1, 2017 10:53 AM
A pro-life group filed a lawsuit Wednesday against Miami University of Ohio, claiming the school unconstitutionally required them to post trigger warnings before erecting a display on campus.
Students for Life of Miami University of Ohio, Hamilton regularly sets up its Cemetery of the Innocents display on the campus' Central Quad, erecting small crosses to represent the number of lives lost to abortion since the passage of Roe v. Wade.
[RELATED: Cornell students protest pro-life views as 'traumatizing']
But in October, when Students for Life President Ellen Wittman contacted the university to schedule the routine event, she was informed that this time, the group would have to post signs on campus beforehand notifying the community of the impending display.
A university official told Wittman she feared the display would cause "emotional trauma," offering to meet with the group to discuss "less harmful" ways of expressing its views.
Students for Life, however, argued that the administration was presenting unnecessary obstacles to erecting the display in an effort to stymie controversy.
Since the incident, the Alliance Defending Freedom has sued the university on behalf of Students for Life, claiming school policy effectively allows the administration to place a "trigger warning" on the pro-life club.
[RELATED: Pro-choice group says pro-lifers provoke 'pain and stigma']
"No university official has the authority to censor student speech simply because of how someone might respond to it," ADF Legal Counsel Travis Barham said in a press release. "The First Amendment secures the freedom of all students to participate in the marketplace of ideas, and it prohibits university officials from imposing trigger warnings that restrict what some students can say to spare the feelings of others."
Similarly, ADF Senior Counsel Casey Mattox noted that "today's university students will be tomorrow's legislators, judges, university presidents, and votes," and "that's why it's so important that public universities model the First Amendment values they are supposed to be teaching to students."
"It should disturb everyone that Miami University of Ohio and many other universities are communicating to a generation that the Constitution doesn't matter," he added.
The lawsuit itself argues that the university's authority allows administrators to "deny permits for any reason, including unconstitutional viewpoint discrimination, and to control the message students convey by imposing conditions on approving these permits."
[RELATED: Video shows TA stealing pro-life poster]
Director of University News and Communications Claire Wagner, however, told Campus Reform that the lawsuit is an "unfortunate misunderstanding," but pledged that "if mistakes were made, they will be addressed."
"All Miami University students and student organizations have First Amendment rights to free speech," she confirmed. "As a result, the university does not approve or disapprove of any student organization's display based on content or subject matter. Miami University does not require trigger warnings."
Follow the author of this article on Twitter: @AutumnDawnPrice
Autumn Johnson
Contributing Writer, Media Research Center
Autumn Johnson is a contributing writer for Media Research Center and an Associate Attorney The Stanley Law Group. Formerly, she was a social media editor for the Blaze, and a Virginia Campus Correspondent for Campus Reform. Autumn graduated from Liberty University School of Law.


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Rod Laver Arena crowned Australia's second top-selling arena
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Melbourne's favourite arena ranks the second busiest venue in the country and 21st internationally, in Pollstar's Top 200 Arena Venues chart, with 586,506 ticket sales during 2018.
A feat made even more impressive for being achieved while simultaneously undergoing phenomenal renovation, as part of the $338 million second stage of the Melbourne Park redevelopment.
Melbourne Arena also came in at 13th place domestically and 157th worldwide, with 83,774 ticket sales.
Australian venues rank highly in the growing 2018 international concert business earnings.
The ongoing growth of the global live entertainment industry this year saw gross revenue surpass US$2 billion from the Top 10 tours for the first time, with Ed Sheeran attaining the highest gross ever recorded for a touring artist in a single year.
P!NK's Rod Laver Arena performances in July and August earned $27.3 million (US$20.2 million) to rank fifth.
Sheeran and the nine other acts included in the Top 10 on Pollstar's annual Top 100 Worldwide Tours chart all topped $100 million in sales during the year.
Sheeran, along with other Top 10 acts Taylor Swift, Pink, Bruno Mars and Roger Waters, each toured Australasia.
Pollstar's list of the world's top performing concert venues saw New York's Madison Square Garden replace London's O2 arena as the top performing venue with 1,115,480 ticket sales.


ATHENS, Ohio – The Ohio wrestling team (0-4) continued the 2017-18 schedule suffering a close 22-19 loss to the University of Tennessee at Chattanooga (3-3) on Monday (Dec. 18) in Chattanooga, Tenn.
Redshirt freshman Trevor Giallombardo (Gaylord, Mich.) suffered an 18-6 major decision loss to Chattanooga's Alonzo Allen. Allen would earn a takedown early in the first period followed by a four-point near fall. Giallombardo would then earn a point from an escape, but Allen quickly gained control again earning another takedown. Giallombardo would earn an escape once more before the second period began at 8-2. In the second period, Allen would earn a quick escape before earning another takedown. Giallombardo would earn another escape to bring the score to 11-3 before the third period. Allen would earn back-to-back takedowns in the third period, both followed by Giallombardo escapes finishing the match at 17-6 where Allen would earn a point for riding time.
Junior Cameron Kelly (Bellbrook, Ohio) would earn Ohio's first win on the day over Chattanooga's Jake Huffine. In the first period, Kelly would earn a quick takedown before Huffine earned an escape bringing the score to 2-1 before the second period. Kelly would earn an escape to start the third period followed by a two-point near fall to take the match 5-1 and earn another point for riding time at 6-1.
Ohio redshirt freshman Coleton Chase (Lancaster, Ohio) would suffer an 18-1 loss to Chattanooga's Mike Pongraz. Early in the first period, Pongraz would earn a takedown, followed by a two-point near fall. Pongracz would then earn a four-point near fall with 30 seconds to-go in the period, leading 8-0 at the end of the first. The second period would start with a four-point near fall for Pongracz followed by an escape by Chase. Pongracz would earn another two-point near fall followed by a four-point near fall before the second period ended, taking the match 18-1 in a technical fall win.
Redshirt sophomore Kade Kowalski (Nashport, Ohio) would earn a 15-0 technical fall win in seven minutes. The first period would start with a quick takedown by Kowalski followed by a four-point near fall. Before the first period would end, Kowalski earned another two-point near fall to lead 8-0 going into the second. Kowalski would start the second period with an escape followed by a takedown to lead 11-0. Kowalski would earn a point from a stalling call on Debien to lead 12-0 at the end of the second period. In the third period Kowalski earn a takedown early on. Kowalski would earn the win at 14-0 and added a point for riding time at 15-0.
Redshirt senior Cullen Cummings (Woodridge, Ill.) would earn a 6-4 decision win over Chattanooga's Dylan Forzani. In the first period, Cummings would earn a takedown before Forzani would earn a reversal to tie it up at 2-2 before Cummings earned an escape followed by a takedown to lead 5-2 going into the second period. To start the second, Forzani would earn an escape to bring the match to 5-3 going into the third. In the third period, Cummings would earn an escape to lead 6-3 at the end of the match. Forzani would earn a point for riding time, but it would not be enough for the win, giving Cummings the victory.
Redshirt freshman Ben Schneider (Orland Park, Ill.) would suffer a 16-0 technical fall loss to Chattanooga's Chad Pyke. In the first period Pyke would earn a takedown followed by back-to-back four-point near falls to lead 10-0 going into the second. In the second period, Pyke would earn a takedown followed by a four-point near fall to end the match at 16-0 in the second period.
Redshirt junior Austin Reese (Urbana, Ohio) would earn an 18-5 major decision win over Chattanooga's Justin Lampe. In the first period, Lampe would earn a takedown followed by a quick reversal by Reese. Reese would then earn a two-point near fall before Lampe would respond with an escape to bring the scores to 4-3 before the second period. Reese would earn a takedown to start the second period, followed by a four-point near fall to lead 10-3 before the third period. In the third period, Lampe would earn a takedown followed by an escape by Reese. Reese would then respond with a takedown followed by a four-point near fall to take the match 17-5 where he would add a point for riding time.
Redshirt freshman Hunter Yeargan (Willard, Mo.) would suffer a 12-3 decision loss to Chattanooga's Bryce Carr. The first period would go scoreless. To start the second period, Carr would earn a takedown before earning a point due to a stalling call on Yeargan to lead 3-0 before the third period. Yeargan would earn an escape to start the third period followed by a takedown by Carr and another escape from Yeargan. Carr would then earn another takedown followed by another escape from Yeargan where Carr would respond with another takedown to lead 10-3. Carr would earn a point on another stalling call and reached 12 with a point from riding time to take the match.
Freshman Aaron Naples (Brunswick, Ohio) would suffer an 11-3 major decision loss to Chattanooga's Scottie Boykin. The match would begin with a takedown by Boykin followed by an escape by Naples. Boykin would then earn another takedown followed by an escape by Naples which Boykin would respond to with another takedown to lead 6-2 before the second period. The second period would go scoreless. In the third period, Boykin would earn an escape right away followed by a takedown and an escape by Naples. Boykin would then earn a takedown to take the match 11-3.
Redshirt junior Zack Parker (Felton, Del.) would earn Ohio's last victory on the day, earning a 15-3 major decision win. In the first period, Parker would earn a takedown early on before Stacey would earn an escape. Parker would then earn another takedown to lead 4-1 going into the second period. The third period would start with a takedown by Parker. Parker would earn a point on a stalling call on Stacey to lead 8-1 before the third period. In the third period, Parker would earn a takedown to start followed by an escape by Stacey to bring the score to 10-2. Parker would quickly earn another takedown before a stalemate was called. Stacey would then earn an escape before Parker earned a final takedown to take the match with a point added for riding time at 15-3.
Ohio is set to continue action on the road when the Bobcats compete in the 55th Annual Ken Kraft Midlands Tournament in Evanston, Ill. Action is set for December 29-30.