records should be inspected and which ones?"
the sample can provide useful and valid information about the entire organization or unit.
quality of the data, and the resources (money and time) available for data collection.
table. The second method is to pick every sixth name (300/50 = 6) starting anywhere in the list.
records could be selected from each grouping to reach diagnostic conclusions about each of the groups.
begins to ensure that every possible problem is corrected.
you can't control it," says Meg Whitman, CEO of eBay Inc.
truth, is also an important factor.
given cost and time constraints. The following criteria lay out some guidelines.
suggests that data collection usually takes longer than planned.
needed for a reliable measure?
content analysis and force-field analysis.
to determine whether some answers are occurring over and over again.
Second, based on this sampling of comments, themes are generated that capture recurring comments.
aspects of work, and the second two address the resources available for doing the work.
with the most responses represent those themes that are most often mentioned.
into two major categories: forces for change and forces for maintaining the status quo or resisting change.
most powerful. One can either rank the order or rate the strength of the different forces.
increasing the forces for change.
tied to an employee's absentee rate), to reduce restraining forces, or to add new driving forces.
calculations are not discussed here.
rewards. Monetary rewards, such as pay and fringe benefits, are not as highly valued.
Table 5. Descriptive Statistics of Value of Organizational Rewards.
the frequency distributions of the responses to the questions about pay and praise from the supervisor.
their standard deviations and frequency distributions suggest different interpretations of the data.
Table 6: Frequency Distribution of Responses to "Pay" and praise from Supervisor" items.
issue posed by the question if the standard deviation is small; the data are similar on a particular measure.
intersection is shown on Figure 29as an X.
suggests that change orders may contribute to the observed conflict between the two departments.
of 0 implies a "shotgun" scattergram where there is no relationship between two variables.
would be valid and useful.
differences between an accounting department and a sales department can be determined with this tool.
compute a difference score (t-score or z-score) indicating whether the two groups are statistically different.
likely that one group is more satisfied than the other.
calculated. The larger the score, the more likely that the group actually changed its job satisfaction level.
purposes of diagnosis or evaluation.
scale on efforts warrants a special look.
content of diagnostic feedback are described below.
activities can increase the relevance of the data.
members verify whether the feedback data accurately represent organizational events or attitudes.
should be limited to what employees can realistically process at one time.
something about because it will energize them and help direct their efforts toward realistic changes.
Comparative. Feedback data can be ambiguous without some benchmark as a reference.
members a better idea of how their group fits into a broader context.
point for more in-depth discussion of organizational issues.
and the hope that desired changes will result from the feedback meeting.
important objective of the feedback process is to ensure that organization members own the data.
change is lost and members will have difficulty engaging in a meaningful process of change.
to use the data for change, then the information will not be owned by the client system.
happens, the energy gained from dealing directly with the problem is lost.
because participants cannot address the problem(s) suggested by the feedback.
address change, there will be little ownership of the data.
Process help. People in feedback meetings require assistance in working together as a group.


The Uncle Eric books have been voted a first place winner in the Government category of Mary Pride's 2018 Practical Homeschooling Reader Awards! as well as an award winner for High School Government and Economics in the Best of 2019 Teach Them Diligently Homeschool Family Favorites Guide.
"Your books (the Uncle Eric books) are among the few I recommend for every high-schooler as 'must reads' before graduation."
America's Founders were most concerned with establishing a government that ensured the greatest economic prosperity and the greatest liberty for individuals. The structure of government that they created was designed to curb the ever-encroaching nature and growth of government, which the Founders believed was the basic nature of all governments. Most civics/government books do an adequate job of explaining the structure of America's government. However, what is often lacking is the "nature" of government - which was of major concern to America's Founders. The following books provide the side of the story (the nature of government) that is most often missing from government/civics textbooks.
These books are best suited for most students ages 14 and up.
These two titles explain the economic model that Mr. Maybury has found most reliable for restricting government.
These two titles explain the legal model that Mr. Maybury has found most reliable for restricting government.
Are You a Liberal? Conservative? or Confused?
These two titles explain which political philosophies are most consistent with free markets and higher law principles, and what plans the promoters of the various political philosophies have for your money and your liberties.
These two titles provide an example of what happens when a society abandons free markets and higher law principles and replaces these with fascism.
This title is included due to the chapter titled "Capitalism and Other Isms."
Mayhew was pastor of West Church in Boston in 1750. Mayhew's sermon points out that there is a Higher Law than any government's law. His sermon encouraged his listeners to choose between Higher Law and their government's law, when one was in conflict with the other. John Adams recommended that everyone read it. So does Richard Maybury.
For our suggested order of reading, please click here.
If you have questions about study time or units, please click here.
This package includes one PDF download. You will receive a link via email that will allow you to download Jonathan Mayhew's Sermon as a PDF.
The purchase of this package entitles you to one download. You have permission to print one copy for your personal reading. You will have 10 days, and three attempts, to download this product before the link expires.


Over the past 12 years Women's Health Action have produced breastfeeding posters we currently have two A3 sized posters in stock.
Tumeke: A beautiful A3 sized poster of a painting by Māori artist Theresa Reihana. Produced by Women's Health Action in partnership with Te Oranga Te Rarawa.
Outstandingly Healthy: A vibrant A3 sized promoting continuation of breastfeeding after introduction of solids. The poster also provides factual information about the energy input of breastmilk for children from 0 – 2 years.
Stickers with the international symbol for breastfeeding in Te reo Māori and English.
Breastfeeding and working for employers: A pack providing key information for employers seeking to provide a breastfeeding friendly workplace.
Breastfeeding and working for employees: A pack providing key information on returning to work and breastfeeding.


Mr. Pachkowski was one of the founding directors of and currently serves as Chairman of the Board of Total Energy Services Inc., having served as a director since 1996. Mr. Pachkowski is a Principal of Trident Capital Partners LP, a management and investment limited partnership and was formerly a partner with Peat Marwick Thorne (now KPMG LLP). Mr. Pachkowski received a Bachelor of Commerce degree from the University of Calgary in 1977 and has been a Chartered Accountant since 1979, a Chartered Business Valuator since 1989 and received his ICD.D designation in 2012.
Mr. Halyk is the founder of and the President, Chief Executive Officer and a director of Total Energy Services Inc. having served as a director since 1996. He has been Chief Executive Officer since May 2002 and President since June 2007. Since December 1997, Mr. Halyk has been a Principal of Trident Capital Partners LP; a private limited partnership that makes investments primarily in the energy, energy services and real estate industries. Prior to starting Trident Capital, Mr. Halyk was a corporate securities lawyer with Bennett Jones LLP in Calgary. Mr. Halyk received his Bachelor of Commerce (Finance Major) and Bachelor of Laws degrees from the University of Saskatchewan.
Most recently, Mr. Melchin served as a Member of the Legislative Assembly of Alberta from 1997 to 2008. For seven years, he served as a Minister of the Crown, including Minister of Revenue from 2001 to 2004 and Minister of Energy from 2004 to 2006. Mr. Melchin left elected office in March of 2008 and currently serves as Chairman of the Board of Enmax Corporation, a municipally owned utility, and on the Board of Directors of Total Energy Services Inc. and Baytex Energy Corp. Mr. Melchin received his Chartered Accountant designation in 1980 and his FCA designation in 2004. Mr. Melchin also received his Institute of Corporate Directors designation in 2007.
Mr. Whitmarsh has over 20 years of energy industry experience. Most recently, from 2008 to 2017, Mr. Whitmarsh served as the Chief Executive Officer of Trinidad Drilling Ltd. Prior to that, starting in 2000, Mr Whitmarsh held various positions with Trinidad Drilling Ltd. including President, General Manager and Field Supervisor. Mr. Whitmarsh holds his Institute of Corporate Directors designation.
Prior to his retirement in 2016, Mr. Smith was a Senior Audit Partner with KPMG. He currently is a director of Razor Energy Corp. and a member of that company's Audit Committee, Governance and Reserve Committees and the Chair of the Audit Committee. Mr. Smith graduated from Concordia University in Montreal, Quebec in 1977 with a Bachelor of Commerce, Honours in Accountancy with Distinction and received his Chartered Accountant designation in 1979. Mr. Smith received his Institute of Corporate Directors designation in 2014.
Mr. Chow was one of the founders of Savanna Energy Services Corp., most recently serving as Executive Vice President of Savanna Energy Services Corp. from 2003 until 2015. Prior to that, Mr. Chow held various executive roles including Vice President, Corporate Development and Vice President and Chief Operating Officer with Plains Energy Services Corp. and President of Entest Corp as well as various public and private directorships including Interra Energy Services Ltd., Profound Energy Inc., Find Energy Ltd., Plains Energy Services Corp. and others. Mr. Chow holds a Bachelor of Laws degree and a Bachelor of Science, Mechanical Engineering with Distinction degree, both from the University of Alberta. Mr. Chow also received his Institute of Corporate Directors designation in 2010.
Mr. Draudson has more than 20 years of business and public company experience in the energy industry and was a founding member of Savanna Energy Services Corp., most recently serving as Executive Vice President, Finance and Chief Financial Officer from 2009 until 2015 and Vice President, Finance and Chief Financial Officer from 2001 until 2009. Prior to that Mr. Draudson served as the Vice President, Finance of Savanna Energy Services Corp. and Chief Financial Officer of Plains Energy Services Ltd. and worked for several years in public practice for a large public accounting firm. Mr. Draudson received his Bachelor of Commerce degree from the University of Calgary and is a Chartered Accountant.
Mr. Brooks has over four decades of experience in the oilfield services, energy, and investment industries, including extensive international expertise. His various roles have included Energy Securities Analyst, Manager of an oilfield services company, and Management Consultant to various energy companies. Mr. Brooks is currently an adviser to Parks Paton Hoepfl & Brown, a boutique oilfield service investment banking firm based in Texas, and is a member of the Board of Directors for several oilfield service companies including Trican Well Service Ltd., and Pason Systems Inc.


This week's DVD and Blu-ray releases are not overly exciting, but there should be something for every fan in need of a new home video purchase.
Due Date is the week's featured new release, but Memento might be the re-release of the week. It is a disappointing week, but there is plenty to be excited about in weeks to come.
Due Date - Todd Phillips brought two of the funniest actors in Hollywood together for a road trip that had the makings of comedy gold. Unfortunately, it passed through as a mediocre buddy comedy that only mustered up a few scenes that were actually funny. Ben Kendrick's 3.5-star review shares his thoughts on an "entertaining experience that's working too many angles – and, as a result, loses momentum at times."
Once the comedic grace period of Zach Galifianakis and Robert Downey, Jr. wears off, we are left with a movie that just tries to hard. The film's strongest moments are Galifianakis' stretch as an aspiring actor and the frequent cameos. The film did its job for the studio with a solid $209 million return at the box office, but that might not be enough to push home video sales.
The DVD and Blu-ray does feature a great video transfer and a worthy audio transfer, but the bonus features are massively underwhelming. Comedies tend to pack a punch in the supplemental category. For some reason, this home video only gives us 16 minutes of weak material.
Weeds: Season Six - Fans of the Showtime series found out late last year that Season Seven will be its last. That means the latest season to hit home video is even more valuable. The series has steadily risen in popularity over the years and it has had a comfortable run on home video.
With 351 minutes of episodes, it is impressive that the season pack costs just under $25, but the rest of the bonus features do not promote such enthusiasm. There are some worthy commentaries, but supplemental features are not extensive.
All-Star Superman - The latest release from DC Universe adapts Grant Morrison and Frank Quitely's acclaimed miniseries. For more, read Kofi Outlaw's full review of the release.
Memento - The film that launched Christopher Nolan's career (after Following) celebrates its 10th Anniversary with a Special Edition Blu-ray release. The re-release includes some new featurettes that expand on the developing legend of the film that works backwards. It may seem hasty for a film to get a 10th Anniversary edition, but few question the validity of Nolan's early masterpiece.
The most disappointing aspect of the special edition Blu-ray is its lack of the DVD's chronological order function. The special edition DVD actually gives viewers a chance to watch the movie in the order it was shot. The re-release features some new supplements mixed with the mainstays from previous releases.
The Stieg Larsson Trilogy - We have not covered the Swedish version of Stieg Larsson's Millennium trilogy as much as some fans would like, but now the trilogy hits Blu-ray and it deserves everybody's attention. As production progresses on David Fincher's remake of the Swedish hit, the original trilogy can finally rest on Blu-ray for home video eternity.
The Girl with the Dragon Tattoo, The Girl Who Played with Fire and The Girl Who Kicked the Hornet's Nest all come to DVD and Blu-ray with nothing more than their individual Blu-ray releases offered. Essentially, if you had the patience to hold off until all three films come in one box set, you'll get nothing extra. Still, any fan of the franchise will have no excuse not to own the original Swedish trilogy on Blu-ray.
Next week has plenty of new and old titles hitting DVD and Blu-ray. The Disney classic Bambi reaches homes in a Diamond Edition Blu-ray re-release, while new releases like Megamind and 127 Hours also hit shelves.
There are more titles to keep an eye out for, but stay tuned next Tuesday for those details.
Do any of this week's home video releases interest you? Will you be holding off for next week?


text:
Q: SSH On Another Port Debian 7.7 I cannot seem to SSH into my server on another Port. I have an identical setup on another server, but I am baffled now if my issue is with IPTables or something else. Does anyone see what I am doing wrong?
I am trying to connect via $ ssh user@site.com -p 555 but I keep getting connection refused.
Here are my IP Table Inputs
Attempt 1
This is what I've added (Saved with the iptables-persistent package)
$ sudo iptables -A INPUT -p tcp -m state --state NEW --dport 555 -j ACCEPT

Attempt 2
And I've tried this, and it seems to list in netstat | grep 555 better than the above
$ sudo iptables -A INPUT -p tcp --dport 555 -j ACCEPT

The IPtables List
Then I list it out and it appears to be in there
$ sudo iptables -L
Chain INPUT (policy ACCEPT)
target     prot opt source               destination
ACCEPT     tcp  --  anywhere             anywhere             state NEW tcp dpt:555

Here is my SSHD config
$ head /etc/ssh/sshd_config
# What ports, IPs and protocols we listen for
Port 22
Port 555
# Use these options to restrict which interfaces/protocols sshd will bind to
#ListenAddress ::
#ListenAddress 0.0.0.0 <--- This has never been an issue for me

Restart SSH
Then I run
$ sudo /etc/init.d/ssh restart

A: I would try disable/stop iptables completely and see if you can SSH in then. There may be another rule which is blocking it.
If it's still blocked, try the following on the actual server.
telnet localhost 555

to check the port is open (at least locally, then try site.com/the public IP).
You can also check SSH is listening via
ss -plant | grep ssh

EDIT: Although it seems to be listening from the looks of the netstat output. I'd say it's the firewall blocking SSH access. If possible, flush ALL the rules and then go from there.

redpajama_set_name: RedPajamaStackExchange


Linfield take on Crusaders on Saturday 21st October 2017. Linfield have 0 this year and Crusaders scored 0 goals. In defence Linfield have conceded 0 and Crusaders have conceeded 0 goals.
Linfield have won 2 out of the last eight league games. Crusaders have 3 victories from the last 8 league games.


text:
Home World Biden Orders Intelligence Inquiry Into Origins of the Coronavirus
Biden Orders Intelligence Inquiry Into Origins of the Coronavirus
The intelligence on the three workers came from outside the United States intelligence agencies' own collection, which means its veracity is more difficult to authenticate. The source of the information was unclear, but several American officials said they believed the report that the three researchers got sick.
American intelligence officials do not know whether the lab workers contracted Covid-19 or some other disease, like a bad flu. If they did have the coronavirus, the intelligence may suggest that they could have become sick from the lab, but it also could simply mean that the virus was circulating in Wuhan earlier than the Chinese government has acknowledged.
Also toward the end of Mr. Trump's term, State Department officials began examining the origins of the virus and concluded that it was highly unlikely to have appeared naturally and thus was likely the product of laboratory work.
CNN first reported the effort and suggested that the group's efforts had been shut down by the Biden administration, prompting scathing Republican criticism. A State Department spokesman, Ned Price, denied that, saying that the team's findings were briefed to senior officials in the department's arms control bureau in February and March.
"With the report delivered, the work was ended," Mr. Price said.
Mr. Trump issued a statement on Tuesday boasting of his early insistence that the Wuhan lab was the source of the virus. "To me, it was obvious from the beginning," he said. "But I was badly criticized, as usual."
Despite the absence of new evidence, a number of scientists have lately begun speaking out about the need to remain open to the possibility that the virus had accidentally emerged from a lab, perhaps after it was collected in nature, a lab origin distinct from a creation by scientists.
"It is most likely that this is a virus that arose naturally, but we cannot exclude the possibility of some kind of a lab accident," Dr. Francis Collins, the director of the National Institutes of Health, told senators on Wednesday.
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redpajama_set_name: RedPajamaCommonCrawl


text:
Q: Checking the values of cell contents in VBA I'm new to using VBA, and I wanted to know if something is possible. I have two workbooks in excel and they are quite large. What im trying to do is reference the values in a certain column, which are all phone numbers, in workbook one and find the matching value in workbook two. then when the matching value is found in workbook two copy the contents of a different cell in workbook one and paste them in a cell in workbook two. Any help would be greatly appreciated.

A: Yes it is possible.  You could do something like the following:
Sub findAndPaste()
Dim row1 as Range, wb1 as Workbook, wb2 as workbook, sh1 as Worksheet, sh2 as Worksheet
Dim value1 as String, row2 as Range, value2 as String
Set wb1 = Workbooks("Workbook1Name")
Set wb2 = Workbooks("Workbook2Name")
Set sh1 = wb1.sheets("SheetName")
Set sh2 = wb2.sheets("SheetName")
For each row1 in sh1.usedrange.rows
    value1 = row1.cells(1).value
    For each row2 in sh2.usedrange.rows
        value2 = row2.cells(1).value
        If value2 = value1 then
            row2.copy row1
            Exit For
        End if
        next 'row2
    next 'row1

End Sub

A: If the question is "Is this possible", then the answer is yes. Also if that is the only question, this is not a programming question.


text:
namespace BiosOpenCV {

  class FindContours : public ProcessFilter {

    private:
      ContourFinder contourFinder;

    public:
      FindContours(const cv::Mat& original, cv::Mat& result, double minimumArea=3000, double maximumArea=15000);

    public:
      virtual void execute(void);

    public:
      std::vector<cv::Point2f> get_centers(void);
  };

};

redpajama_set_name: RedPajamaGithub


I'm just a mama, looking to get less fat than I was last week. I am not a tire flipping, barbell lifting, competition maniac. What the hell I am getting into?
"I just got passed by a 10 year old. Ok, this is a little embarrassing better get moving."


text:
Frank Orth (February 21, 1880 – March 17, 1962) was an American actor born in Philadelphia. He is probably best remembered for his portrayal of Inspector Faraday in the 1951-1953 television series Boston Blackie.

Career
By 1897, Orth was performing in vaudeville with his wife, Ann Codee, in an act called "Codee and Orth". In 1909, he expanded into song writing, with songs such as "The Phone Bell Rang" and "Meet Me on the Boardwalk, Dearie".

His first contact with motion pictures was in 1928, when he was part of the first foreign-language shorts in sound produced by Warner Bros.  He and his wife also appeared together in a series of two-reel comedies in the early 1930s.  Orth's first major screen credit was in Prairie Thunder, a Dick Foran western, in 1937. From then on, he was often cast as bartenders, pharmacists, and grocery clerks, and always distinctly Irish.

He had a recurring role in the Dr. Kildare series of films and also in the Nancy Drew series as the befuddled Officer Tweedy.  Among his better roles were the newspaper man Cary Grant telephones early in His Girl Friday, one of the quartet singing "Gary Owen" in They Died with Their Boots On (thereby giving Errol Flynn as Gen. Custer the idea of associating the tune with the 7th Cavalry), and as the little man carrying the sign reading "The End Is Near" throughout Colonel Effingham's Raid. However, Orth is probably best remembered for his portrayal of Inspector Faraday in the 1951-1953 television series Boston Blackie. A short, plump, round-faced man, often smoking a cigar, Orth as Faraday wore his own dark-rimmed spectacles, though rarely in feature films.

In 1959, Orth retired from show business after throat surgery. His wife died in 1961 after around fifty years of marriage.

Death
Orth died on March 17, 1962. He is buried in Forest Lawn Cemetery in the Hollywood Hills next to his wife.

Selected filmography

The Painter (1931)
Sleepy Head (1931)
Dumb Luck (1931)
The Unwelcome Stranger (1935) - Jackson
The Payoff (1935) - Porter (uncredited)
Two Against the World (1936) - Tommy - Bartender
Hot Money (1936) - Hank Ford
Polo Joe (1936) - Bert
Land Beyond the Law (1937) - Deputy Shorty Long
San Quentin (1937) - Convict in Bunkhouse (uncredited)
Fly-Away Baby (1937) - Newsreel Reporter at Airport (uncredited)
Ever Since Eve (1937) - Cocktail Waiter (uncredited)
Marry the Girl (1937) - Barman (uncredited)
Talent Scout (1937) - Burlesque Theatre Manager (uncredited)
The Devil's Saddle Legion (1937) - Judge Barko
The Footloose Heiress (1937) - Justice Abner Cuttler
Prairie Thunder (1937) - Wichita
Submarine D-1 (1937) - Roseland Waiter (uncredited)
Missing Witnesses (1937) - Gordon (uncredited)
The Patient in Room 18 (1938) - Joe Higgins
Torchy Blane in Panama (1938) - First Leopard (uncredited)
Flight into Nowhere (1938) - Hammond's Associate (uncredited)
Little Miss Thoroughbred (1938) - Justice of the Peace (uncredited)
Mr. Chump (1938) - Sheriff Frank Hinton
Young Dr. Kildare (1938) - Mike Ryan (uncredited)
Nancy Drew, Detective (1938) - Captain Tweedy
Comet Over Broadway (1938) - Cab Driver (uncredited)
Burn 'Em Up O'Connor (1939) - Tim 'Mac' McKelvy
Idiot's Delight (1939) - Benny Zinsser (uncredited)
Fast and Loose (1939) - Detective Hendricks (uncredited)
Nancy Drew... Reporter (1939) - Capt. Tweedy (uncredited)
Within the Law (1939) - Jim Jenks
Society Lawyer (1939) - Man in Outer Law Office (uncredited)
Winner Take All (1939) - Pete - Bettor (uncredited)
Broadway Serenade (1939) - Mr. Fellows
Calling Dr. Kildare (1939) - Mike Sullivan - Hospital Cafe Owner (uncredited)
Tell No Tales (1939) - Vic - Bartender (uncredited)
Young Mr. Lincoln (1939) - Loafer (uncredited)
Stanley and Livingstone (1939) - Newspaperman with Pills (uncredited)
Nancy Drew and the Hidden Staircase (1939) - Captain Tweedy
Thunder Afloat (1939) - Old Sailor in Bilge Area (uncredited)
Dust Be My Destiny (1939) - Wedding Witness on Stage (uncredited)
Fast and Furious (1939) - Captain Joe Burke
At the Circus (1939) - Cook in Diner (uncredited)
The Secret of Dr. Kildare (1939) - Mike Sullivan
His Girl Friday (1940) - Duffy
Dr. Kildare's Strange Case (1940) - Mike Ryan, Sullivan's Hospital Cafe
'Til We Meet Again (1940) - Hong Kong Bartender (uncredited)
The Doctor Takes a Wife (1940) - New York Editor (uncredited)
La Conga Nights (1940) - Dennis O'Brien
Florian (1940) - Detective (uncredited)
Brother Orchid (1940) - Waiter at Fat Dutchy's (uncredited)
Gold Rush Maisie (1940) - Harris
Pier 13 (1940) - Dead Pan Charlie
Boom Town (1940) - Barber
Dr. Kildare Goes Home (1940) - Mike Ryan (uncredited)
The Bride Wore Crutches (1940) - Bartender (uncredited)
Mexican Spitfire Out West (1940) - Window Washer (uncredited)
Gallant Sons (1940) - Newspaper Foreman (uncredited)
Father Is a Prince (1940) - Drugstore Proprietor
Dr. Kildare's Crisis (1940) - Mike Ryan, Cafe Owner
Michael Shayne, Private Detective (1940) - Steve
Let's Make Music (1941) - Mr. Botts
Come Live with Me (1941) - Jerry
The Strawberry Blonde (1941) - Baxter - Livery Stable Owner (uncredited)
Road Show (1941) - Joe - Lion Owner (uncredited)
Ride on Vaquero (1941) - Auctioneer (uncredited)
The People vs. Dr. Kildare (1941) - Mike Ryan
The Great American Broadcast (1941) - Counter Man
Broadway Limited (1941) - Lew, Roundhouse Cafe Owner (uncredited)
Sergeant York (1941) - Drummer (uncredited)
Kisses for Breakfast (1941) - T.C. Barrett, the Hobo (uncredited)
Dr. Kildare's Wedding Day (1941) - Mike Ryan
Navy Blues (1941) - Joe (uncredited)
I Wake Up Screaming (1941) - Cemetery Caretaker
They Died with Their Boots On (1941) - Barfly (uncredited)
Skylark (1941) - Subway Cashier (uncredited)
Unholy Partners (1941) - Shino McGoon (uncredited)
Blue, White and Perfect (1942) - Mr. Toby
Right to the Heart (1942) - Pete
Dr. Kildare's Victory (1942) - Mike Ryan
Roxie Hart (1942) - Idler (uncredited)
Rings on Her Fingers (1942) - Kellogg
To the Shores of Tripoli (1942) - The Barber (uncredited)
My Gal Sal (1942) - McGuiness
Henry and Dizzy (1942) - Joe McGuire
The Magnificent Dope (1942) - Messenger
Little Tokyo, U.S.A. (1942) - Jerry
Footlight Serenade (1942) - Mike the stage doorman
Tales of Manhattan (1942) - Secondhand Clothes Dealer (Rogers sequence)
Orchestra Wives (1942) - Rex Willet
Springtime in the Rockies (1942) - Mr. Bickel (uncredited)
Dr. Gillespie's New Assistant (1942) - Mike Ryan
Over My Dead Body (1942) - Detective
The Meanest Man in the World (1943) - NYC Bartender (uncredited)
Hello, Frisco, Hello (1943) - Lou, Bartender at Sharkey's
The Ox-Bow Incident (1943) - Larry Kinkaid (uncredited)
Coney Island (1943) - Saloon Waiter / Member of quartette, 'Irish' number (uncredited)
Sweet Rosie O'Grady (1943) - Taxi Driver
Buffalo Bill (1944) - Sherman - Shooting Gallery Owner
Roger Touhy, Gangster (1944) - Comic in Theater (uncredited)
Summer Storm (1944) - Cafe Maitre d' at End (uncredited)
Wilson (1944) - Smith (uncredited)
The Impatient Years (1944) - Counterman (uncredited)
Greenwich Village (1944) - Ordway (uncredited)
Tall in the Saddle (1944) - 'Shorty' Davis (uncredited)
Storm Over Lisbon (1944) - Murgatroyd
Carolina Blues (1944) - Cab Driver (uncredited)
The Captain from Köpenick (completed in 1941, released in 1945) - Knoll, the Factory Personnel Manager
Pillow to Post (1945) - Clayfield Taxi Driver
Wonder Man (1945) - Charlie - the Bartender (uncredited)
Tell It to a Star (1945) - Augustus T. Goodman
The Lost Weekend (1945) - Opera Cloak Room Attendant
The Dolly Sisters (1945) - Stage Manager at The Bijou (uncredited)
She Went to the Races (1945) - Bartender Skelly
Doll Face (1945) - Peters
Colonel Effingham's Raid (1946) - Wild Man (uncredited)
The Hoodlum Saint (1946) - Chronicle Editor (uncredited)
Blondie's Lucky Day (1946) - Shaving Kit Salesman (uncredited)
Murder in the Music Hall (1946) - Henderson, the Stage Manager
The Bride Wore Boots (1946) - Judge (uncredited)
The Well-Groomed Bride (1946) - Waiter (uncredited)
The Strange Love of Martha Ivers (1946) - Hotel Clerk
It's Great to Be Young (1946) - Franklin Johnson
Wake Up and Dream (1946) - Milkman (uncredited)
The Show-Off (1946) - Mr. Kopec - Superintendent (uncredited)
Lady in the Lake (1946) - Floyd Greer (uncredited)
Born to Speed (1947) - Breezy Bradley
The Guilt of Janet Ames (1947) - Danny
Heartaches (1947) - Mike Connelly, Vic's Agent
Mother Wore Tights (1947) - Stage Doorman (uncredited)
Gas House Kids in Hollywood (1947) - Police Captain
It Had to Be You (1947) - Train Conductor Brown
The Big Clock (1948) - Burt
Fury at Furnace Creek (1948) - Evans (uncredited)
So This Is New York (1948) - A.J. Gluskoter
The Girl from Manhattan (1948) - Oscar Newsome
Family Honeymoon (1948) - Candy Butcher (uncredited)
Blondie's Secret (1948) - Mr. Philpotts
Make Believe Ballroom (1949) - Pop (uncredited)
Red Light (1949) - Stoner
Bride for Sale (1949) - Police Sergeant (uncredited)
The Great Rupert (1950) - Mr. Frank Dingle
Cheaper by the Dozen (1950) - Higgins (uncredited)
Father of the Bride (1950) - Joe
The Petty Girl (1950) - Moody
Double Dynamite (1951) - Mr. Kofer
Something to Live For (1952) - Waiter (uncredited)
Houdini (1953) - Mr. Hunter
Here Come the Girls (1953) - Mr. Hungerford
Not as a Stranger (1955) - Greenville Patient (uncredited)
Hold That Hypnotist (1957) - Beedle - Man in Library (uncredited)

References

External links

American male film actors
1880 births
1962 deaths
Burials at Forest Lawn Memorial Park (Hollywood Hills)
Male actors from Philadelphia
Vaudeville performers
20th-century American male actors

redpajama_set_name: RedPajamaWikipedia


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Think of the most iconic brands you know: what makes them so memorable? Is it the colors, the words, or a combination of both? The story a company tells about itself is the foundation upon which successful brand recognition is built, and the words and visuals are what tell this story. Join Forum One branding experts to learn the essential content and design elements for building a brand.
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Home / Columns / Thomas Hauser
Fighting for America
Thomas HauserJan 29, 2003by Rish Administrator
By Thomas Hauser
On November 23rd, DiBella Entertainment will present "Fighting For America," featuring nine professional bouts at Roseland Ballroom in New York. James "The Hammer" Butler, Brian Adams, and Olympians Jermain Taylor, Clarence Vinson, and Brian Viloria will headline the card. One hundred percent of the gate receipts will be donated to the Twin Towers Fund established by the City of New York to assist the families of police, firefighters, and other government personnel who died in the September 11th tragedy.
The three Olympians will donate their purses in full to the fund. Tickets will be priced from $50 to $200 and may be purchased through TicketMaster by calling 212-317-7171. Thomas Hauser has been asked to write the feature story for the night's fight program. SecondsOut readers get to see it first.
I'm very down on the overall state of boxing. From top to bottom, the sport is a mess. The business is endemically rotten. People lie and misrepresent and cheat as much on twenty-thousand-dollar club-fight cards as they do on the multimillion-dollar pay-per-view shows. There's endless scheming and plotting to hurt other people. The negative energy is overwhelming. One of the few good things about it for me so far has been dealing with the fighters. A lot of people told me that being on the side of the business that I am now would teach me that the fighters are as bad as everyone else. But that hasn't been the case. So far, I've found most of the fighters to be very special decent individuals. There have been disappointments, but I love working with people like Bernard Hopkins and Naseem Hamed. I enjoy the mentoring aspect of my relationship with the Olympians. There are times when I'm proud to be a member of the boxing community, and November 23rd will be one of them. -- Lou DiBella.
Roseland, where tonight's fights are being held, stands a block from Broadway. For a long time, in New York's theater industry when someone died young, the question asked was, "Did he die of AIDS?" Now, for a horrible 10 weeks, when we hear of someone dying young, the question asked is, "Did he die at the World Trade Center?"
The human mind is capable of conjuring up evil that, for some of us, is literally unimaginable. Any individual can end another person's life and wreck havoc on the lives of the victim's loved ones. A small group of plotters, indeed a lone assassin, can cause incalculable harm.
Once something has happened, we can't change it. We can't undo what has been done.
Yet one day can change the world. It happened in 1914, when Archduke Ferdinand was assassinated in Sarajevo, lighting the conflagration that became World War I. It happened at Pearl Harbor. And it happened again 10 weeks ago.
On September 11th, we all died a little. America was the target, but the entry point for the wound was New York.
We are aware of the twin towers more now in their absence than ever before. We're also uncomfortably aware of the fact that evil lurks just beneath the surface of our lives. There are places in the world where the type of violence we've just experienced is part of everyday life. But Americans have been largely immune to such cares. Most of us have had the luxury of going through life confident that the sudden ending, the tragic accident, the unspeakable horror, won't happen to us. To a friend; perhaps. To someone we know; probably, as a matter of statistical likelihood. But not to me.
Now the certainty we seek in our lives has been undermined, and in its place we have fear. The fear that someone wants to kill us; the fear that, just as terrorists were trained as pilots here in the United States, their brethren might be learning the nuances of lethal biology at universities and laboratories in America; the fear that this is the start of a long hard difficult time.
Many of us have been traumatized by the visual image of United Airlines Flight #175 hitting the south tower of the World Trade Center more than we understand. "People keep saying 'like a movie, like a book,' Stephen King wrote recently. "And I keep thinking, 'No, not at all like a movie or a book. This is what it really looks like when an actual plane filled with actual human beings and loaded with jet fuel hits a skyscraper. This is the truth."
That scene will stay with us forever. It is the signature image of horror in our times.
Now comes the response. Makeshift shrines to honor lives lost have sprung up outside fire stations across New York. People seem friendlier, warmer, and more polite. They smile and nod in acknowledgement to one another more now than before. They look at cops and firefighters with new appreciation and respect.
One of the ironies of our democratic society is that, in recent decades, only one segment of society has been called upon to do the hard dangerous dirty work. In World War II, people of all classes fought in the military. But since then, America's armed forces have been comprised largely of men and women who come from poverty and see the military as a form of upward mobility.
The World Trade Center attack struck at Americans of all classes. With terrorism, we are all soldiers. And on September 11th, we were reminded of who our real heroes are. Now, we say to the perpetrators of this horror as Winston Churchill once spoke to Adolph Hitler, "We will have no truce or parlay with you or the grisly gang who do your wicked will. You do your worst, and we will do our best."
That brings us to tonight's fights. Everyone talks about how superstar athletes are heroes and role models. But most professional athletes, like the rest of us, do first and foremost for themselves.
The fighters on tonight's card are making a true sacrifice. Like cops and firefighters, boxers know what reality is. In our video-game culture, every time they enter the ring they put themselves on the line. They know that a single moment of violence can change everything. Yet many of the fighters here tonight are entering the ring for free, and their opponents are appearing for greatly reduced purses. That's part of the good in boxing.
I've often said that, if I went to war, I'd want professional fighters beside me. Professional fighters and trainers like Teddy Atlas and a young Eddie Futch. I still believe that to be true. Meanwhile, conventional wisdom dictates that a fighter isn't considered truly great until he has gotten up off the canvas to win.
New York will rise from the canvas and win.
To contact the author, email: thauserthauserrcn.com
GGG Gennady Golovkin displays renewed speed under new trainer Johnathon Banks (video)
Kid Galahad has NOTHING BUT BOXING in his life and that will be crucial vs Josh Warrington
Errol Spence vs Terence Crawford: Bernard Hopkins breaks it down
Boxing, Bud and the Big Apple


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Drunk doctor allegedly kills patient during operation in Zimbabwe
A medical doctor in Zimbabwe, Henry Harry Mushuku of Gutu Mission Hospital, has allegedly killed a patient during a childbirth operation. The deceased identified as Locadi Mbindi died after the delivery of her baby two weeks ago.
According to a Zim Eye Report, Dr. Mushuku conducted the operation under the influence of alcohol resulting in the death of the patient.
But commenting on the incident, Gutu District Medical Officer, Dr. Tapiwa Mupepe, said the woman died of delivery complications.
'The woman died as a result of delivery complications and we are in the process of analysing the matter.' he said.
Masvingo Provincial Medical Director, Dr. Amadeus Shamu who also confirmed the incident said investigations were ongoing.
'I have heard about the incident but let me say investigations are under way. I can furnish you with further details after receiving a comprehensive report on the matter.'
It's however understood that the medical doctor was under the influence of alcohol when he operated on her.


This is to express our profound shock and dismay with your recent decision not to commute the life sentence of Jonathan J. Pollard. This is a missed opportunity to right a wrong and to render justice where it is long overdue.
As I have followed this matter closely, I am convinced that the alleged damage to national security from Mr. Pollard's actions never occurred. In fact, our friend and ally Israel was enabled to defend herself and in turn protect U.S. interests.
For historical perspective, Mr. Pollard should be viewed in the context of the close strategic cooperation between the United States and Israel and the many contributions of Israeli intelligence to America's security; release of the 1956 Krushchev speech on the crimes of Stalin, the first captured MIG-21 and the sharing of intelligence on Soviet weapons systems and air defense capabilities following the 1966 Six-Day War, the 1973 Yom Kippur War and the 1982 Peace for Galilee action, among others. The 1983 U.S.-Israel Memorandum of Understanding, which Pollard's immediate superiors ignored, arose out of this context. Thus, Pollard in fact may deserve our thanks for helping reduce the threats to U.S. and Israeli interests in the Middle East.


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News & EventsNews & Events
Helping Council Apply for Bridge FundingHelping Council Apply…
Back to News Items
Helping Council Apply for Bridge Funding
Monday 1st of June 2020
Paddy's Flat Road in the Tenterfield Shire Council area is an important freight transport route running 60kms from the Bruxner Highway at its southern end, to the Tooloom Road at its northern end. The road provides transport and passenger vehicle access to Tenterfield, Casino, the North Coast and south east Qld.
There are a number of important industries along the route including a large blueberry farm, avocado growers, cattle producers and tourism businesses. Numerous farm inputs also must travel along the road. There is a significant NSW Forestry Corporation and private forestry resource (26,300 hectares) which currently cannot be harvested or thinned due to load limits on two key bridges on Paddy's Flat Road – one 3kms from the intersection with the Tooloom road, and one 3kms from the intersection of the Bruxner Highway. Council have upgraded other key bridges along the road and these two remaining bridges now remain a major impediment to utilising the forestry assets, and allowing the use of larger trucks to improve the freight efficiency task for other businesses as well tourist buses bringing school students from Qld to the Clarence camping facility which must travel an additional 2 hours.
Council are applying for around $780,000 of government funding under the Australian Government's to replace both bridges, raising the load limit such that B-double and semi-trailer trucks can operate. This will dramatically improve the efficiency of both the inward and outward freight tasks and open up access to a stranded forestry resource which presently cannot be managed properly or harvested as thinning and clearfall products cannot be transported out.
Our team are assisting council with the funding applications by conducting the Benefit-Cost Analysis economic modelling for the two bridge projects. Increasingly, councils are being asked to provide economic evidence that their road upgrades make sound economic sense and Benefit-Cost Analysis is the standard method of providing this evidence.


Hackers will hunt for easy targets. Don't be the lowest hanging fruit and make it hard for hackers to get in.
Not having the right measures in place to counter and instantly respond to cyber attacks.
Current security measurements are not functioning as expected and you are visible to hackers. The hackers are exploiting business software that is also implemented in your organisation.
Your focus is not Cyber Security which makes it hard to improve, since you dont have to knowlegde and/or time to increase the levels of Cyber Security measurements.
Hackers will continue attacking you since the implemented measurements do not fit the current problems and challenges in cyber security for your organisation.
Create traps in your network to catch hackers. Contain them, analyze the attack and follow up. Hackers don't like aggressive reactions. And most importantly they dont want to be exposed.
Attracts hackers and interacts with them.
Keeps them contained as long as possible.
Generates information to file reports and improve security measurements.
Hackers tend to attack the easiest targets they can find. By implementing multiple security measurements and answering agressively to hackers, you will become unattractive to attack for hackers.


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Looks Like Florida Decided Tampons Weren't A 'Luxury' After All!
No more tampon tax in Florida? Way to go Sunshine State!
Rebekah KuschmiderJess Lahitou
AtHomePundit
Tampons in Florida are about to get cheaper — 6% cheaper to be exact!
Why? Well, the governor of Florida just signed a package of tax cuts into law, and one of the changes to the state tax code is eliminating sales taxes on tampons, pads, menstrual cups and other menstrual supplies.
You see, a lot of states exempt certain necessities like food and medicine from sales taxes — that extra few percentage points added at the register when you buy products. For some reason, menstrual supplies got left off the list of necessities in a lot of these states. Maybe because the people writing the tax code were mostly of the variety of people who never had periods? A solid guess, but we can't say for sure.
Anyway, for the past few years, people who menstruate have started agitating to get supplies for taking care of periods designated as tax-exempt because HELLO! Periods happen! The blood has to go somewhere. For most people, that "somewhere" is a maxi-pad, tampon, or menstrual cup. That means paying for those supplies every time your period comes. One estimate suggests that menstruating consumers pay about $7 per month for menstrual supplies. Every month. For about 40 years. That's $3,360 for tampons and stuff over a lifetime. Taking 6% off that — as in the case of the Florida sales tax — would be a savings of $201 per period-having-human!
And let me reiterate that this isn't a choice. Periods are a naturally occurring bodily function. They just happen and ya gotta deal with it. Every single time.
Florida joins 13 other states and the District of Columbia in making menstrual supplies non-taxable. This has been a bipartisan issue, probably because members of both parties are better educated about the realities of menstruation than previous generations were.
Other states will likely follow suit over the next few years, but don't look for federal legislation making menstrual supplies tax-free. Sales taxes are a state issue, so our friends in D.C. can't help us on this one.
taxes on menstrual supplies
eliminate tampon tax
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This Bamboo Party Plate is a great option for entertaining. This square plate has an opening to hold stemware or a can or bottle. It is 8" square and 3/8" thick. There is room for laser imprinting along the raised edge.
Imprint area along raised edge: 1/4" x 4", Laser.


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Florida State Seminoles News
by Brian Geisinger Tuesday May 14, 2019
Stock Rising: Can FSU's Devin Vassell emerge as one of the top 3-and-D prospects in the country?
Devin Vassell
Florida State Basketball
Trent Forrest
Florida State guard Devin Vassell, right, shoots over Gonzaga guard Josh Perkins during the first half an NCAA men's college basketball tournament West Region semifinal Thursday, March 28, 2019, in Anaheim, Calif. (AP Photo/Marcio Jose Sanchez)
The 2019 NBA Combine takes place this week up in Chicago. Over 20 percent of the players invited to participate in this year's event (14) played in the ACC last season. Most of those guys have already committed to staying in the draft; Jordan Nwora of Louisville is the one prospect that's left the door for a possible return open.
Other ACC players received G League Elite Camp invitations, but could also decide to return to college, too. Virginia's Mamadi Diakite is a prime example of this — along with Oshae Brissett of Syracuse and Duke's Javin DeLaurier. There are also prospects, like NC State's Markell Johnson, who didn't receive an invite to either event, but are testing the waters and working out with teams.
For this exercise, though, we will look at one of the top under-the-radar draft prospects in the country, one that we know will be back in the ACC for the 2019-20 season — Florida State's Devin Vassell.
Out Front
On the surface, Vassell's numbers don't exactly pop. That's mostly because Vassell, as a freshman, played less than 11 minutes per contest in a crowded wing rotation: 4.5 points and 1.5 rebounds per game. One can easily expect that to change in the 2019-20 season, though, especially with Terance Mann (1,174 minutes) and Mfiondu Kabengele (27.5 percent usage rate) off to the NBA — along with the departures of PJ Savoy, Phil Cofer and David Nichols.
That's a lot of touches, possessions and minutes — and experience — that just left town. This means two guys with serious NBA potential — Trent Forrest and Devin Vassell — will get a full season together to showcase what they have to offer to NBA teams. (MJ Walker will factor into this equation, too.)
https://accsports.com/wp-content/uploads/2019/05/vassell-dunk-GT.mp4
Devin Vassell: 3-and-D Target
The sample size is on the smaller side, but for the season, Vassell shot 41.9 percent on 3-point attempts: 26-of-62 (7 3PA per 40 minutes). In ACC action, that number jumped to 44.4 percent on looks from downtown (7.6 3PA per 40 minutes). Pair that shooting efficiency with his frame (6-foot-6), and you have the outline of an intriguing 3-and-D prospect.
https://accsports.com/wp-content/uploads/2019/05/vassell-cs-3-GT-1.mp4
Vassell averaged two steals and 1.1 blocks per 40 minutes, too. According to Bart Torvik's site, he was the only ACC player — among those that played in at least 20 percent of their team's minutes — to shoot 40 percent on 3-pointers, and have a 2.5 percent block rate and a 2.5 percent steal rate.
Most of his work from deep, unsurprisingly, came off the catch. According to Synergy Sports, Vassell scored 1.4 points per possession (70.2 eFG%) on no-dribble jumpers from spot-up possessions. That ranked No. 5 in the ACC (minimum 40 possessions), behind only Ty Jerome, Ty Outlaw, Markell Johnson and Coby White.
Mostly playing on the second side of the offense, over half of Vassell's field goal attempts this season were 3-pointers (52.1 percent); in ACC play, his 3-point attempt rate jumped to 66 percent.
Furthermore, every single one of his 26 3-pointers were assisted on, including this bomb from the ACC Tournament over a Nickeil Alexander-Walker closeout.
https://accsports.com/wp-content/uploads/2019/05/vassell-transition-cs-3-VT.mp4
Dual-Level Shot Profile
While the majority of his shot attempts came from beyond the arc, Devin Vassell showed some off-ball mover skills that resulted in finishes from other levels of the floor, too. Vassell shot 63.2 percent at the rim (24-of-38 FGA), per Bart Torvik's site, though his half-court number at the basket are less desirable, according to Synergy.
https://accsports.com/wp-content/uploads/2019/05/vassell-alley-oop-Canisius.mp4
If Vassell, who had six dunks this season, could unlock more gravity as an off-ball cutter and off-screen shooter, it would have a profound impact on his outlook as a prospect. Florida State doesn't run a lot of off-ball motion actions, but that's Vassell's next step. Right now, he's mostly a standstill shooter from deep.
https://accsports.com/wp-content/uploads/2019/05/vassell-cut-Canisius.mp4
Event though he hasn't shown much of it yet, that doesn't mean it isn't there. Vassell plays with good instincts and body control. Again, in a relatively small sample, Vassell showed some ability to attack the offensive glass (4.3 percent offensive rebound rate) and finish. He was 5-of-7 on put-back attempts (71.4 FG%) after an offensive board, per Synergy.
https://accsports.com/wp-content/uploads/2019/05/vassell-putback-dunk-ND.mp4
Defensive Upside
It's hard to find a trustworthy listing online of Vassell's wingspan, but he clearly has the ability to contest shots on closeouts and disrupt passing lanes. In his 353 minutes of action this season, Vassell proved to be a quality stocks (combined steals and blocks) player. He averaged over three stocks per 40 minutes this season, which is really solid.
Devin "Young Mikal Bridges" Vassell
— Jackson Hoy (@jacksonghoy) March 23, 2019
With Mann and Forrest prowling around, Vassell defended more secondary options as a freshman, though he did spend a little bit of time on Ja Morant during Florida State's NCAA Tournament win over Murray State. (He was one of, like, six or seven FSU players that checked Morant in the first half, alone.)
Forrest is a bulldog defender, and he's back for another season of hounding opponent ball-handlers. Vassell offers serious switch potential, though. He can guard four positions in college, and the rising sophomore wing should draw more primary assignments, too. Regardless, his focus should remain on being a quality off-ball help defender — don't ball watch, rotate, closeout hard.
https://accsports.com/wp-content/uploads/2019/05/vassell-block-GT.mp4
That stuff isn't exciting for most to talk about, but it matters: finding ways to contribute as a good team defender can raise the floor of a prospect. Plus, with his wingspan and activity level, the blocks and steals will find themselves.
https://accsports.com/wp-content/uploads/2019/05/vassell-steal-runout-VT.mp4
Pairing With Forrest
Florida State will really miss the impact offense Kabengele provided around the basket; when things bogged down this season, FSU could look for the big fella at the rim (64.6 FG%) or let him get to work on the offensive glass (11.5 percent offensive rebound rate). That's a luxury they won't have in the 2019-20 season — complicated by the absence of Mann and his slashing, crafty power game.
Terance Mann: 70% at the rim on 538 attempts in 4 years. That … is not normal. 115 dunks at 6'6" in 4 years.
— Ross Homan (@Ross_homan1) May 11, 2019
For FSU to generate offense, a great deal of play-making responsibilities will fall on the shoulders of Forrest. Fortunately for Leonard Hamilton's crew, Forrest is a wickedly physical and intelligent player. Over the last two seasons, Forrest averaged 5.6 assists per 40 minutes; he has finished two straight seasons with assist rates above 23 percent.
He also joins Tre Jones and Jose Alvarado as the only three ACC returnees for next season to post an assist rate of 20 percent and a steal rate of three percent during the 2018-19 season.
Forrest will need to cut down on his turnovers, although his turnover rate dropped to under 17 percent in ACC play, which is solid. Regardless, that dude loves to get downhill and play slash-and-kick basketball; this makes for a promising pairing in the half-court offense with Vassell.
https://accsports.com/wp-content/uploads/2019/05/vassell-cs-3-VT-Forrest-pass.mp4
It can't be all on Forrest for offensive creation, though, and Vassell may have to showcase more off-the-dribble juice as a sophomore. According to Synergy, Vassell was just 1-of-11 (9.1 FG%) on half-court off-dribble jumpers this season.
However, this type of catch-and-shoot action could be the bread-and-butter of Florida State's half-court attack. In terms of skillsets, it's right in the wheelhouses for both Vassell and Forrest, too.
Vassell posted a turnover rate of just 10.3 percent this season, which is great; but that's with him functioning as shooter/ball-mover on offense. That's a number to monitor next season, especially if his role changes significantly in Florida State's offense.
Read More on Devin Vassell and FSU Basketball
Trent Forrest returns to FSU as a part of a stacked 2020 ACC point guard class
1 Number To Know, ACC Prospects in the NBA Draft: Mfiondu Kabengele and Terance Mann
FSU super-sub Mfiondu Kabengele declares for the 2019 NBA Draft
Winningest season in Florida State history ends with Sweet 16 defeat to No. 1 Gonzaga
More Florida State Insider and Recruiting News


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Culture 19 articles Operations 22 articles Performance 14 articles Guides 3 books
Jack Enright
Staff Writer @ CharlieHR
Running a small business?
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How to conduct an exit interview – including example exit interview questions
When an employee decides to leave your business, it's important for you to try and find out why – if you can understand their reasons for resigning, you're much better placed to encourage others to stay. Your opportunity for finding those answers comes during the exit interview. In this article, we'll cover exactly how to conduct an exit interview as an employer – including some great exit interview question examples.
What is an exit interview?
An exit interview is a conversation that happens between a company and an employee who has decided to leave the business. It's helpful to imagine them as the opposite of a job interview - instead of asking why they want to join your company, you're asking them why they've decided to leave. The objective is to better understand why that employee has decided to resign, in the hope that the company can improve how it works and prevent other employees from following suit.
Why are exit interviews important?
A great many people within HR say that exit interviews are among the most important conversations you'll ever have at work (although Charlie's COO actually disagrees). It will be one of the most honest conversations you have with your employees; your one opportunity to find out what your team is really thinking.
And in a very basic sense - if someone is leaving your company, it's important to know why. People rarely decide to leave jobs for trivial reasons. If they've gone down this path, it's likely that some substantial aspect of their role was leaving them dissatisfied in some respect.
If you want to keep your best employees and attract even more, then it's crucial for you to find out why they would leave, and also what might make them stay. A properly run exit interview gives you the perfect opportunity to find out.
How to conduct an exit interview
Now that we know what they are for, we're going to talk about how to conduct an exit interview as an employer. The following are some key best practices it pays to keep in mind while conducting an exit interview, as well as some example exit interview questions.
First off - every leaver gets an interview
Every person that leaves your company deserves an exit interview. There are a few practitioners out there who will tell you to only conduct them with your star performers (the ones you really want to keep) and not to worry about 'trouble-makers'.
Marking out exit interviews as valuable only for certain parts of the business sends out a really damaging message. It tells the rest of the company that only some people's views are worth listening to. The reason exit interviews exist is to give you a clear oversight of everything that goes on at your company - not just the impressions of a few.
If you're discarding the experiences of so-called 'trouble-makers' then you're creating a massive structural blindspot. You're going to miss out on the crucial information that could tell you why that hire turned into a 'troublemaker' in the first place - perhaps there is something wrong with the way that role is managed that makes it harder for people to succeed. Unless you ask, you'll never know.
Be timely
The best time to hold an exit interview is on the leaver's last day, or in the days immediately following their departure. Before this, they aren't going to be as free with their thoughts.
A few days worth of 'mental distance' from the role is especially valuable. It gives the leaver something of a vantage point, from which they can make a more critical assessment of their experiences rather than one based on emotions.
Try not to leave it too long though - longer than a week and they'll start to feel disengaged from the business, particularly if they've already started in a new role. You might find their feedback becomes quite general or less direct.
Keep it transparent
Always share the topics that you'd like to cover in advance. Make a list of the questions you want to ask or areas you'd like to tackle, then drop it round to the leaver in an email ahead of time.
Not only does this help to put them at ease, but it also makes it more likely that they'll come back with some thoughtful, well-developed answers. It gives them a few days to mull over their experiences and come back with some considered feedback, rather than have to come up with something on the spot.
It pays to make exit interviews as relaxed as possible. At Charlie, we try to make sure they aren't held in the office but somewhere more informal, such as a nearby cafe. This helps to reinforce the idea that this is an amicable, two-way conversation between ex-colleagues, rather than some form of disciplinary.
If you're going to run the interview at work, it's inevitably going to feel like they're at work.
Keep it personal
One interviewer is always enough. You don't want them to feel like the company is ganging up on them - and as much as you can, let the leaver decide who they'd like to conduct the interview. You'll receive much more honest (and therefore more valuable) feedback if the two get on well together.
That being said, it's well worth trying to make sure that the interviewer is at least 'one-remove' higher than the leaver, rather than their direct line manager. There's a couple of reasons for this.
Firstly, it helps the leaver to be a bit more honest with their feedback. There's an old saying in HR circles that says: people don't quit jobs, they quit bosses. This is your chance to see if that's true of your business.
Secondly, it automatically shifts that feedback one step further up the hierarchy of the business, making it more likely that action is taken on it.
Listen more than you talk
As the interviewer, don't feel that you have to respond to feedback - that's not what this process is for. You're not there to defend the business or justify your own decisions, but to learn as much as you can about your company.
This could be a really tough experience - particularly if you're a founder. But take heart in the fact that even the most damning feedback can be acted upon to make your company better.
"Exit interviews can be really difficult, especially if the company is your own. It's a conversation where someone basically has free reign to have a go at you and your company... It can feel like you're being kicked whilst your down. But the trick is to just swallow your pride, and let them have their say." Ben Gateley, CEO at CharlieHR.
Don't lead the conversation, guide it
As the interviewer, you might feel a temptation to lead the conversation to the topics you badly want to talk about. This is especially true of founders, who often feel passionately about the business they have built and have strong ideas about how they think it should be run.
However, it's a mistake to seize control of this conversation. By all means, guide it to the topics you think might be relevant, but the goal is to hear what the leaver has to say - so let them take the interview where they want to take it.
You may find that it yields unexpected and surprising answers.
At Charlie, we make sure that the notes from exit interviews are shared across the whole company leadership, not just amongst the leaver's immediate team. This helps to ensure that company leaders have a clear understanding of how every side of the business is performing.
...then act on them.
Everything up until this point has been the easy part. Now you need to actually act on what you've learnt.
If you've run the exit interview properly, you may well have heard a few things that made you feel uncomfortable - tales of team members not pulling their weight or managers who aren't supporting their staff.
It might make for difficult listening, but if you're serious about improving the way your business is run then you need to commit to making the changes that are needed.
Example exit interview questions
What do we need to improve as a company?
What would it have taken to convince you to stay?
What do we do well?
Did you feel properly supported by your manager?
Did you feel you received good Learning and Development opportunities?
What's the hardest thing about working here?
As far as you can, treat the leaver with respect and gratitude for the work they have done with you. They'll go on to work at many other places, with many other people, and you want them to leave your company as an advocate and ambassador for you and the way you work.
There is an instinct to be defensive about exit interviews - after all, someone that you hired has decided to quit, and is about to tell you every single reason for that decision. Yes, staff exits mean that your company is changing, but as long as you are in control of the direction of travel that is a good thing.
CharlieHR builds software to help small businesses thrive. From tracking time off, running performance reviews or keeping tabs on employee engagement, Charlie provides everything you need to manage your people – all in one place.
You can try it free today by clicking here.
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This page links to some of my presentations and standard "modules" delivered to various audiences. I am available to create or deliver presentations on other Israel-related topics, both historic and current affairs, customized to any specific audience and length.
The Israeli-Palestinian Conflict: Territorial or Existential?


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Jérémie and Caroline
Many people have asked about my son Christopher's friend who was shot in both legs during the Paris terrorist attacks on 13 November. Where she was that night and how she is doing now.
Though I wasn't planning to write a blog today, Christopher sent me an article from Le Monde a few days ago and this is a story that needs re-telling.
Le Carillon, 14 November 2015
In the article*, written by Isabelle Rey-Lefebvre, Jérémie Zerbit, a pharmacy student, recounts that he was sitting inside Le Carillon, at the back, when the gunmen attacked. At first inert from shock at the carnage around him, he was roused by others, including the handful firemen who arrived quickly on the scene. They assigned him to help a young woman, conscious but sitting in a pool of blood from gun wounds to both legs. It was Caroline, Christopher's friend.
Jérémie used his belt as a tourniquet for one leg, a tea towel for the other. Leaving aside his own phone that was vibrating constantly with messages from worried friends, he called Ruter, Caroline's boyfriend, to reassure him that she was alive. Then they waited for the ambulances.
In Jérémie's own words to the journalist:
"She told me that she was called Caroline, that she'd just returned from Iran...
"...I'll never forget that young woman's courage. I was surprised by her smile, her gentleness and her patience. She saw my distress and it was she who encouraged me, who helped me help her. I ventured a look outside and saw all the bodies covered with white sheets, some wafting in the wind, exposing disfigured faces. People like me, young...I was undone and Caroline said: 'Don't look.' To distract me, she even asked: 'And you, Jérémie, what do you do in life?"
Doctors from the nearby St Louis hospital arrived and Jérémie persuaded Caroline to take some morphine. Finally, after an interminable hour-long wait, the ambulances arrived. Jérémie gathered Caroline's belongings, put her shoe in her bag, and she was taken away.
Caroline was operated on the next morning. Once released from the hospital, she went with Ruter to her parents' in Normandy. Fortunately, no bones were hit and she will walk again. In fact, she told Jérémie on the telephone, she's planning to dance in 2016.
If Jérémie hadn't helped Caroline, she may well have bled to death. And had Caroline not helped Jérémie, he may well have fallen apart.
Many thanks to you both, Caroline and Jérémie, for showing human nature at its best, in the midst of its worst.
*unfortunately, the on-line version of the article, published 24.11.15, is locked into a subscriber's fortress and I can't even provide the link. Nor will my pdf copy reproduce here.


WARMTH & CHARM greet you on the front porch of this lovely 3 bed/2.5 bath home in sought-after Concord Walk. Inside you'll be drawn to the inviting fireside family room & spacious separate dining room. You'll love cooking your favorite dishes in the large kitchen with granite counters & tiled backsplash & then sharing them with friends & family in the beautiful four-season sun room with views to the landscaped garden. Upstairs you'll find a master bedroom & bath complete with whirlpool tub and double vanities then two more bedrooms with Jack & Jill bath.


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Home National Ivanka Trump whitewashes her dad's latest sexist attack with a family friendly...
(AP Photo/Susan Walsh, File)
Ivanka Trump whitewashes her dad's latest sexist attack with a family friendly op-ed
Alison R. Parker
The White House may be eager to distract the public from Donald Trump's most recent burst of misogyny, but Ivanka Trump's op-ed supporting national paid family leave can't hide her father's sexist nature.
Donald Trump's recent repulsive attacks on MSNBC's Mika Brzezinski offered further proof — as if any more were needed — of his deep-seated and barely contained sexism.
But ever his valiant defender, his daughter Ivanka Trump did her part to attempt to convince the nation that her father's administration really, truly cares about women and families.
In an op-ed at the Wall Street Journal, Ivanka pushed for a national paid family leave policy, touting her father's proposed budget for "emphasizing the need for mothers and fathers to have access to paid leave to encourage both parents to share parenting responsibilities and to strive toward minimizing hiring biases."
She declared that the policy would have "an especially positive effect for women," who are more likely to have to leave their jobs to care for a baby or child.
And she tied the issue to the wage gap, as well:
Additionally, making it easier for new parents to return to work after the arrival of a new baby is a critical part of solving the persistent gender and minority pay gap that exists in part because of prolonged periods away from the workforce and challenges with re-entry.
Incidentally, Ivanka failed to mention that said wage gap is most pronounced within her father's White House.
And while Trump's budget proposal does indeed include six weeks of paid leave to mothers and fathers following the birth or adoption of a child, that meager offering hardly excuses the myriad other policies the administration has proposed or implemented that are detrimental to women and children.
Six weeks of paid leave can't compensate for draconian cuts to Medicaid, defunding attacks on Planned Parenthood, or the massive $2 billion reduction to the Social Services Block Grant, which provides aid to states on issues such as "child care assistance, ... child abuse and neglect, [and] community-based care for the elderly and disabled."
28 million Americans depend on services funded by those grants — half of them children, about whom Ivanka claims her father cares so deeply.
Going after programs like Meals on Wheels, public television, and food stamps further evinces an utter lack of concern for the well-being and thriving of families with less financial means than those who are more well off — like Ivanka's.
And her praise for her father's paid leave policy is certainly not enough to excuse or cloak Trump's rampant, pompous sexist attitude.
Ivanka can write all the op-eds she wants, but her father is still the same man who lobs playground-level insults at female news anchors who don't give him fawning coverage or female candidates who dare to run against him; who treats contestants in beauty pageants like little more than livestock or objects for his amusement; who proudly boasted about getting away with sexual assault; and who had the galling audacity to insist that, as president, he would be better for women than Hillary Clinton.
Six weeks of paid leave aside, nothing Trump has done thus far in office offers any support for that incredulous claim.
Trump's disregard for the concerns of women, and his overarching sexism, is part and parcel of who he is, no matter what ostensibly "women-friendly" policies he deigns to include in his budget — or how much his dutiful daughter elevates him with undeserved accolades.
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Reproductive justice and LGBTQ rights activist; freelance writer. Follow her on Twitter @alisonrose711.


Please pray for our dear friend's son Luke Souza. The Souza's have been friends of our family's for 40+ years. I believe Luke is only 20 years old. A couple of weeks ago, he was diagnosed with Hodgkin's Lymphoma. The good news is, the doctors are optimistic that it is "highly curable with chemotherapy and possible radiation." Nevertheless, we encourage you to join us in praying for Luke's complete healing and the families peace and comfort as they go through this season.
You can also let Luke and the family know they are not alone (that we are with them), by visiting Luke's Caring Bridge site at caringbridge.org…Luke Souza. Please leave a comment of encouragement and support. We know firsthand what it means from Ryan's Caring Bridge site and this one. In case you don't know, it means the world to us.
OH! And may God be Praised in 2018!!
Praying for Luke and his family, and always for Ryan and your family.
Thank you Sherry! I know the family will be pleased to hear of your support.


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FUTURE OF PARTY WINGS IN MALAWI
November 15, 2019 November 15, 2019 M Khundira
By Francis Botha
Politics and participation are fundamental to sustainable development. Political parties in Malawi have lived to address that yet their youth and women wings continue to face complex barriers towards their attainment of meaningful political participation. They are still dependent on their mother parties for resources.
The oldest party, the Malawi Congress Party (MCP) under late Hastings Kamuzu Banda formed the Youth and Women's League. The advent of multiparty democracy, under Bakili Muluzi with United Democratic Party (UDF) founded both women and youth wings (Young Democrats) but without sustainable policy. Most of them later defected to the DPP.
Youth wings
A youth wing is an extension of a party that provides space to youths to participate in matters of the party. It counters declining youth membership and serves as political leadership nurseries. An ideal youth wing serves to influence policies and development activities.
One good example is the membership of South African politician, Julius Malema, who had been a member of the African National Congress Youth League (ANCYL) from the age of nine until his expulsion from the party in 2012 at the age of 31. Today Malema commands the voice of youths in South Africa and beyond.
Students' wing
Students' wings can promote politics of inclusiveness and tolerance. University students can help parties tackling critical education issues or advance change agenda. In 1993, scores of university students helped to unseat dictator, Kamuzu Banda, through mass protests. The expected voices of the students' activism should be to push government to take positive steps to implement higher education policy with adequate resources.
However, the role of student supporters from regimes has been to detract public attention from pertinent issues. Students should watch against the toxic influence of political parties infiltrating their wings.
Women Wings
The under-representation of women at any level of governance and decision-making results in a democratic deficit. A women wing aims to promote women interests within the party structures without male hindrances. In some parties, all women automatically become members, while in others it is optional. Equal participation and representation should start at grassroots in order to give women recognition. Women should strive to lobby for quotas in their parties at different levels of governance. However, gender inequality and financial constraints make them fall prey to party loyalty.
Influence of CSOs and parties
Good governance starts with well-structured wings at grassroots level that receive support from other stakeholders. Parties must give wings political mandate to participate in the electoral processes and hold offices at all levels. The role parties ascribe to women and youths determines their political empowerment and engagement. The European Union Delegation to Malawi funds Young Women in Active Politics (YWAP) project aimed at grooming young women from the ages of 15 to 35 to actively participate in intraparty politics. Centre for Multiparty Democracy (CMD) and Women's Legal Resources Centre (WOLREC) are some of the implementing partners.
A youth led NGO, Youth and Society (YAS) championed the youth manifesto towards the May 21, 2019 tripartite elections, which according to its Executive Director, Charles Kajoloweka, has positively influenced party policy directions. He believes government has positively responded to the manifesto by allocating at least K3 billion towards youth entrepreneurship.
"When young people get organised with clear policy demands and build energy around it, they get positive response from government," says Kajoloweka.
Challenges faced by wings
Resources are the main constraints facing wings. Due to dependence on the mother party, they have little influence on party policy formation. Youths critical to agendas of the mother party are denied powers to influence policy for fear of alienating older voters. Successive regimes have used youths to unleash terror on innocent citizens. MCP Youth League and its paramilitary wing, the Malawi Young Pioneers (MYP), became the most dreaded agent of Kamuzu Banda's brutality against everyone with dissenting views. They were disarmed in an operation called Operation Bwezani in December 1993.
Malawians hoped the operation was a significant catalyst for transition to democracy. However, UDF's Young Democrats also became synonymous with terror. The current regime, the DPP youth wing, fondly called the 'Cadets' also unleashes terror against those exercising their freedom of speech. It is alleged that some have infiltrated the security organs to crackdown on their opponents.
People's Party's (PP) youth wing was out of power before it was fully-fledged, while the UTM party youths are becoming confrontational against aggression. Remarkable strides have been made in the current parliament of 22 percent female representation with DPP having the youngest parliamentarian; Fainess Magonjwa aged 24. This is record-breaking towards inclusive youth participation in decision-making positions.
Government, CSOs and international organisations seeking to advance the participation of women and youths should invest in grassroots politics or else wings shall remain tools for destruction.
← IMAGINE MUSIC GIANTS FORM ONE BAND
A difficult year →
August 27, 2020 August 27, 2020 M Khundira
That's too extreme Mr. President!
September 23, 2022 October 7, 2022 Nancy Ngoma


Starrah and Diplo have followed up their September EP release with a visual treatment for the projects lead single "Zoo." In under three minutes, we see Diplo and Starrah as cartoon characters partying and flying into space with some cute zoo animals. Some animal cops at first seem like they want to break up the fun but after having some candy they head to space to join in on the party. This collaborative tape, titled Starrah x Diplo, is the first time the singer has really put her vocals on the forefront since she's been known as a songwriter for Rihanna's "Needed Me," Drake's "Fake Love," and a number of Travis Scott's hit songs.


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FUNDACIÓN PARA LA CONSERVACIÓN DE LA BIODIVERSIDAD ACUÁTICA Y TERRESTRE (FUCOBI)
FUCOBI
Miembros de FUCOBI
mangroveENCODE
Biodiversidad de Manglares de Ecuador
Proyecto sobre la Absorción de CO2 por los Manglares
Químicos Perturbadores Endócrinos (EDCs) en Manglares
shrimpENCODE
Biodiversidad y Diversidad Genética del Camarón
TSV en Camarón
WSSV en Camarón
IHHNV en Camarón
Alérgenos de Camarón
Metales / Cadmio en Camarón
Biodiversidad y Diversidad Genética Pescado
Diversidad Genética de la Concha
Diversidad Genética del Cangrejo
Desarrollo de Camarones Tolerantes a Bioacumulación de Cadmio
Químicos Perturbadores Endócrinos / EDCs en Camarón
Proyecto de Epigénoma del Camarón
childrenENCODE
Somos lo que Comemos
Malformaciones Congénitas (Metales y COPs)
Diabetes y EDCs (metales y COPs)
Cáncer y EDCs (metales y COPs)
Aprenda sobre los Contaminantes que afectan a las Personas
Genes que causan Alergias en Humanos
Resistencia a Antibióticos y la Industria Avícola
Proyectos para Estudiantes
Becas para Estudiantes
Expediciones Científicas
SHRIMP ALLERGENS, ENDOCRINE DISRUPTING CHEMICALS (EDCs) AND TRANSPOSABLE ELEMENT (TE) -LIKE REPEATS: A REVIEW
Jacqueline Presedo1*, Alexandra Bowen1, Laura Hake1, Acacia Alcivar-Warren1-3
1Biology Department, Boston College, Chestnut Hill, MA 02467 USA, 2Environmental Genomics, Inc. Southborough, MA 01772, 3FUCOBI (Fundacion para la Conservacion de Biodiversidad Acuatica y Terrestre), Guayaquil, Ecuador; E-mail: fucobi@gmail.com
Shrimp allergy is one of the most common food allergies in the United States. Shrimp allergy is an IgE-mediated Type I allergy, a long-lasting disorder, which usually persists throughout life, and is often associated with severe reactions, including life-threatening anaphylaxis. The four known shrimp allergens are tropomyosin (TM), arginine kinase (AK), myosin light chain (MLC), and sarcoplasmic calcium-binding protein (SCP). This review seeks to thoroughly examine the current literature about the allergen genes and the potential epigenetic effects of EDCs and TEs on allergen expression. TM (Pen a 1) is the major shrimp allergen and TM (Lit v 1) contains seven IgE-binding regions, AK (Lit v 2) contains seven IgE-binding regions, MLC (Lit v 3) contains five IgE-binding regions, and SCP (Lit v 4) contains three IgE-binding regions.1 There are a large number of allergen-related ESTs for L. vannamei in the UniGene database. MLC is the most variable of these allergens, with six clusters of thousands of related sequences in the UniGene database. TM had two clusters, AK had one cluster, and SCP had two clusters of related sequences. Some of these allergen genes contain repeats that resemble those of vertebrate TEs, which could play a role in the body's recognition of foreign DNA and/or the over expression of the allergen genes. Different TE-like repeats were identified in some of the EST sequences of particular allergens and are being used to develop EST-SSR markers for the linkage map of L. vannamei (ShrimpMap2).2-3 Most of the shrimp consumed in the United States is either farmed or wild caught in developing countries and is often contaminated with pathogens and EDCs, including heavy metals such as cadmium and persistent organic pollutants (POPs), which are known to have negative health effects in most living organisms.4-5 It is possible that contaminants such as viruses and EDCs induce the expression of TEs in the shrimp genome, which in turn affects mRNA expression levels of allergen genes in edible muscle tissues. Stress-sensitive TEs are potential allergy biomarkers. Most of the shrimp allergens appear to have been isolated from cDNA libraries prepared with RNA of specific pathogen-free (SPF) L. vannamei of the US Marine Shrimp Farming Program. Some allergen EST-SSR markers are polymorphic in wild shrimp of Ecuador and in cultured SPF shrimp, making them useful for population genetics, traceability, and molecular breeding to develop a hypoallergenic shrimp line.
Myosin light chain (MLC)
Arginine kinase (AK)
Tropomyosin (TM)
Sarcoplasmic calcium-binding protein (SCP)
1Ayuso et al. 2010. Greater epitope recognition of shrimp allergens by children than by adults suggests that shrimp sensitization decreases with age. J Allergy Clin Immunol. 125:1286. 2Presedo et al. Searching for hypoallergenic shrimp: Polymorphisms in expressed sequence tags (ESTs) homologous to shrimp allergens. IMSEGI Session, Aquaculture America 2011, New Orleans, Feb 28-March 3, Abst. 492. 3Bowen et al. Transposable element-like repeats in allergy-causing genes from SPF L. vannamei postlarvae - development of allergen EST-SSRs for linkage mapping. Aquaculture America 2012, Las Vegas, Feb 29-March 3, Abst. 4Keating et al. 2007. Histological findings, cadmium bioaccumulation, and expressed sequence tags (ESTs) in cadmium-exposed L. vannamei postlarvae. J Shellfish Res 26:1225. 5Airaksinen et al. 2011. Association between type 2 diabetes and exposure to persistent organic pollutants. Diabetes Care 34:1972-9.
Fuente: https://www.was.org/WasMeetings/meetings/ShowAbstract.aspx?Id=25577
TRANSPOSABLE ELEMENT-LIKE REPEATS IN ALLERGY CAUSING GENES ISOLATED FROM SPF Litopaneaus vannamei POSTLARVAE EXPOSED TO 1 PPM OF CADMIUM – DEVELOPMENT OF ALLERGEN EST-SSRs FOR LINKAGE MAPPING
Alexandra Bowen1*, Jacqueline Presedo1, Laura Hake1, Acacia Alcivar-Warren1-3
1Biology Department, Boston College, Chestnut Hill, MA 02467 USA, 2Environmental Genomics, Inc., Southborough, MA 01772, 3FUCOBI (Fundación para la Conservación de Biodiversidad Acuática y Terrestre), Guayaquil, Ecuador
A large number of ESTs with homology to the shrimp allergen myosin light chain (MLC) have been reported from cDNA libraries prepared from muscle of specific pathogen-free (SPF) Litopenaeus vannamei exposed to White Spot Syndrome Virus and Taura Syndrome Virus.1-2 The number of people with shrimp allergies in the US has increased in the past 20 years, and most shrimp consumed by Americans is imported. Availability of specific pathogen-free (SPF) L. vannamei, the most widely farmed shrimp species around the world, has helped to control viral diseases. No information on the potential risk of SPF shrimp on increased allergen expression has been reported. The toxic metal cadmium (Cd) is an important environmental pollutant, a carcinogen, a metallohormone, and an endocrine disruptor. Cd accumulates in testes, prostate, renal epithelial cells and liver, binds to DNA, and may cause heritable mutations. Chronic, sublethal Cd levels may be harmful to human health.
Table 1. Expressed Sequence Tags (ESTs) Homologous to Shrimp Allergens in the Litopenaeus vannamei UniGene database.
*Out of 381 (PL42) and 162 (Cd-PL42) Unigene entries. Additional ESTs were isolated but they are not included in the UniGene database – they are homologous to shrimp allergens based on sequence homology searches using Blast est.
Cd increases oxidative stress, induces the expression of transposable elements (TEs) such as LINE non-LTR retrotransposons, may play a role in diabetes occurrence, and exacerbates allergic reactions. Cd accumulates in the body of L. vannamei in proportion to environmental concentrations.3 Baseline expression data on the potential effects of Cd on shrimp health and growth suggested a complex interaction between environmental conditions and host genetic background.3 To begin to understand the molecular mechanisms involved in the increased prevalence of shrimp allergies, basic knowledge of shrimp genome structure is needed. We hypothesize that Cd induces the expression of allergy-causing genes and genome rearrangements mediated by TEs. The specific aims of this study were to (a) determine if the number of allergen genes is increased after Cd exposure, (b) characterize the TE-like repeats in shrimp allergens, and (c) develop EST-SSRs for mapping of shrimp allergens. ESTs were isolated from cDNA libraries constructed using the cephalothorax of SPF, untreated L. vannamei postlarvae stage 42 (PL42) and Cd-treated (1ppm) postlarvae (Cd-PL42). Results showed no difference in the number of allergen genes isolated from control and Cd-treated cDNA libraries from cephalothorax (Table 1). This is contrary to the large number of MLC-related ESTs isolated from the cDNAs cloned using muscle of SPF juveniles challenged with WSSV and TSV. Homology searches showed a larger number of unique EST sequences in Cd-PL42 than in control PL42. These ESTs may represent new TE-mediated loci or TEs, which may play a role in allergic reactions. Most allergen ESTs contained TE-like repeats similar to those found in Ginger2 DNA transposon, endogenous retrovirus (ERV2), and LINE and CR1 non-LTR retrotransposons. Preliminary results on the utility of allergen EST-SSR markers for linkage mapping will be presented.
1Alcivar-Warren et al. 2007. Characterization and mapping of expressed sequence tags isolated from a subtracted cDNA library of Litopenaeus vannamei injected with White Spot Syndrome Virus. J. Shellfish Res 26:1247. 2Alcivar-Warren A, W Warren. 2009. Sex, retrotransposons and disease tolerance – A review of the genetics and genomics of the penaeid shrimp. World Aquaculture Society, Veracruz, Mexico, Sept 2009, Abst #931. 3Keating et al. 2007. Histological findings, cadmium bioaccumulation, and isolation of expressed sequence tags (ESTS) in cadmium-exposed, specific pathogen-free shrimp, Litopenaeus vannamei postlarvae. J. Shellfish Res 26:1225.
This research was funded in part by Environmental Genomics, Inc., the FUCOBI Foundation, Boston College, and USDA-CSREES (through a grant to the USMSFP, to A-W).