Construction artistry! Gino Gazzea inlaid walnut veneer in his Astrale glider to produce this intricate detail. Photo by Eros Cavalarro, at a SAM chapter meet in Rietti, Italy.
there isn't always the return guarantee that you get from MECA members.
The Precision Aerobatics Model Pilots Association (PAMPA) is the organization for CL Precision flying, known as Stunt in the old days. Most CL fliers still build their own models, so you can expect to see some useful material in the bimonthly Stunt News publication. And new members can come up to speed by perusing back issues online.
This monthly publication is only available online. It's an extension of the publication started by the late John Worth and is now edited by Roland Friestad. It features a variety of small models for FF, CL, and RC, powered by engines or electric- or rubber-powered motors. Many are scaled-down versions of older models, but some are originals. Subscriptions start at $24 a year for 12 issues.
Among the July 2013 offerings are the FF Ramrod 250, CL Zilch X-Pendible, and Phil Kraft's RC Gimlet. You'll see plenty of interesting photos, copies of original construction articles, supply sources, and more. Full-size plans are also available as downloads or mailed hard copies.
DVDs displaying some complete issues are also available, and more are being scanned.
The A2Z Corp is a supplier specializing in rubber-powered kits and accessories. The company's website lists its products as well as many from other related manufacturers.
Tandy Walker has set up his own website showing step-by-step methods he used for building several OT models. His aircraft are of museum quality, but do fly.
So far he has reports on 15 models.
50 to 100 numbered progress reports.


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News and TipsTravel News
Southwest Celebrates 50th By Giving Away Millions Of Rapid Rewards Points
Greg Robertson
Phillip B. Espinasse / Shutterstock.com
Airports and Flying
It's been 50 years since Southwest Airlines began flying, and the airline is pulling out all the stops to celebrate its golden jubilee.
Southwest has declared June 18 Wanna Get Away Day, marking its 50th anniversary and giving customers the chance to win a variety of prizes.
"It's our 50th anniversary, and we are ready to celebrate and thank our customers," Bill Tierney, vice president of marketing, said in a release.
Southwest began as a regional Texas airline, flying between Dallas, Houston, and San Antonio when its first planes took off on June 18, 1971. It has since become one of the nation's most successful airlines, flying to 115 destinations in 40 states as well as locations in Mexico, Central America, Puerto Rico, and the Caribbean.
"Customers have the chance to engage with Southwest throughout the month of June, with its biggest surprises occurring on its anniversary and newly branded day," the airline said.
Throughout the month, Southwest will be giving away up to 50 million Rapid Rewards points through the airline's frequent flyer program. Customers will have daily opportunities to win points, gift cards, and companion passes during the promotion.
"In true Southwest spirit, we are turning our 50th anniversary into Wanna Get Away Day, allowing customers the opportunity to celebrate our 50-year history of heart, low fares, legendary hospitality, and flexible policies by giving them opportunities to turn wanna get away into gonna get away," Tierney said.
To get in on the action, customers need to go to the contest page on the Southwest website. From there, they will click on the Enter Now button and see a number of pieces of luggage rolling by on the screen. Players click on three suitcases to see what they'll win.
Southwest promises other promotions will be unveiled as part of the celebration, both online and at the airport, leading up to June 18.
Interested in the state of the airlines? See all our airport and flying coverage here, or start planning a weekend getaway or bucket-list trip with our expert inspiration.
Greg Robertson View Full Profile
Greg Robertson has worked as an editor and writer for some of the top newspapers and websites in the country, leading him to live all across the country. He now calls Las Vegas home, but has journeyed from the sunshine of California and the rain in Seattle, to the humidity of Arkansas and the snow of Minnesota. His travels have taken him to Puerto Vallarta, Costa Rica, Spain, and the Dominican Republic, but he's happiest at home with his wife, two daughters, and dog.
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redpajama_set_name: RedPajamaCommonCrawl


As our basic knowledge regarding genetics increases, DNA holds the necessary information about our genes. It contains the genetic makeup that we inherited from our parents, grandparents, great-grandparents, and so on and so forth.
Our DNA is found inside the nucleus. It is arranged into chromosomes. Each cell contains a DNA plus the genetic information. Before a cell undergoes division or replication, the DNA also divides. Then the protein that is required undergoes the process of transcription in the form of RNA. In the next step, which is processing, non-needed codes are being eliminated which are then removed out of the nucleus which is the process of transportation. Outside the nucleus, the final process of translation occurs as the proteins are being constructed.
To fully understand the difference between the two, the sugar in the DNA and RNA shall be discussed here.
The DNA and RNA have reoccurring nucleotide units. The nucleotide is composed of nucleic acid, a phosphate, and lastly a sugar. The sugar that is found in the DNA is called deoxyribose while the sugar found inside the RNA is called as ribose. The sugar portion of the DNA and RNA are the same. However, ribose contains more oxygen, hydrogen atoms also known as hydroxyl. This is the major difference between the two.
Ribose was discovered in 1891 by Emil Fisher. Ribose is also known as D-ribose. Ribose is very needed in the process of transcription which is stated in the second paragraph. Ribose is also needed by ATP and NADH. These compounds need Ribose for phosphorilation. In the process of phosphorilation, ribose is turned into a functional sugar. Our human body forms ribose through sugar. Ribose is a monosaccharide.
Deoxyribose, on the other hand, was discovered by Phoebus Levene during 1929. Deoxyribose is also called as 2-deoxyribose and dideoxyribose. Deoxyribose is considered a monosaccharide.
Ribose is available in the market. It is manufactured in the form of tablets, powders, and other forms. Deoxyribose is not manufactured in the market.
1.Deoxyribose is the sugar found in DNA while ribose is the sugar found in RNA.
2.Ribose has more hydroxyl atoms than deoxyribose.
3.Ribose is also called D-ribose while deoxyribose is also called 2-deoxyribose and dideoxyribose.
4.Ribose was discovered in 1891 while deoxyribose was discovered in 1929.
5.Both sugars are a form of monosaccharide.
joshua. "Difference Between Sugar in the DNA and RNA." DifferenceBetween.net. April 12, 2018 < http://www.differencebetween.net/science/difference-between-sugar-in-the-dna-and-rna/ >.


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Each Story Is A Dark World In Itself With Its Own Unique Flavour.
Thanks to all five authors for giving me an ARC. The review, however, is not sponsored and the words represent only my personal opinion.
To put it simply, In Ruin is a collection of chapters from five different novels. These aren't short stories but the beginnings of novels around a Dark Dystopian theme, each ending with cliff-hangers. The premise of these stories ranges from War Of the Worlds type Aliens to The Hunger Games type of Dystopian society, all with one or more mature elements including sex (obviously), suicide, murder, brain wipe, hysteria and so on.
Overall, this anthology makes for a good book with mostly gripping penmanship. Stories do have varying degrees of charm so the result is a couple stories where I find myself yearning to read the author's full vision in an upcoming novel, and stories which I would surely give a pass.
The Divine Beasts: M. Reeds' Alien Invasion story describes the horror of murdering, mutilating aliens invading Earth and slaughtering humans. The ease with which the author describes the harrowing events is amazing. You feel the protagonist's tension and gut-wrenching terror of knowing she will die a gruesome death now. The melancholy of lone survival and the defiance in the face of death are very compellingly written.
The second part of the story is completely different, though, and it goes to show the story is but an introduction to this world. The cliff-hanger ending comes at the zenith of explicit tease. This is intriguing enough to look forward to the full story.
Xena's Medallion: Aura Bud's futuristic dystopian divided society story is the best out of the five stories in this anthology in my opinion. Although a bit predictable, the story depicts depths of human emotion very sharply. The emotional tension between two lost lovers and the sexual culmination are very moving and fulfilling. The setup of war for survival keeps the events crisp and the reader's attention taut. The full story is set to release as a novel in 2023 and I am really looking forward to it.
His Alien Flower: N K Allen's Alien, Mind Control, Fated Mate story is the weakest one in the anthology. Mature and explicit sexual contents notwithstanding, the plot drivers and some story elements feel more like young adult fantasy than the dark dystopian fiction that I expected. I shall pass on the full story, if it came out as a novel.
Death of The Angler: T G Grem's world is a mix of Victorian era society with elements of dictatorship and rebellion. The lead titular character is extremely well written in the limited number of pages. And the title: the title is fulfilled in the very last line of the story (or the part of the story that is in this anthology) and it is goosebumps inducing. My second favourite story in the anthology. This is a gripping story even without any explicit or implied sex, but there is no shortage of grit and grue in this story of one person's defiance against a dictator.
The romance element was unexpected and sweet. It is something that will surely feature more prominently in the full novel, but in the core, this is the story of strength in the face of maniacal cruelty.
Curse of Betrayal: Celeste T's Shapeshifter romance has elements of random murder and violence (and sex) - but it lacks depth. The casual outlook of the protagonist actually reflects into the story, too. Out of the five stories, Curse of Betrayal is the only one with some comedy. That is a nice change of pace, but, again, with the limited number of pages, with the incomplete story, it all seemed a bit random.
The tone and the premise aside, the writing is quite good. The cliff-hanger surely left me wanting more but the portion of the story available in this anthology did not, in my opinion do justice to the story title, nor the genre.
With this brief about the individual stories, I must repeat to say that collectively, the book is a gripping read. Although I couldn't finish it in one sitting, I finished it in one day. I would surely recommend it for readers who don't feel squeamish about mature and dark stuff.
The book comes out on 01-Oct-2022


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Discreet Vulgarities
Nicola Kuperus and Adam Lee Miller
Sept 11 – Oct 10
View images from this exhibit
Simone DeSousa Gallery is pleased to present our first exhibition with artists Nicola Kuperus and Adam Lee Miller. In Discreet Vulgarities Nicola Kuperus and Adam Lee Miller's new works walk a thin line between the grotesque and the humorous, incorporating common elements and images such as 2″ x 4″ studs, pieces of plywood, mess-ups and fragments of the body. The new exhibit, including paintings, photographs, sculptures and video, also references situations of the everyday, social taboos, and "purpose."
Extending through both gallery spaces, with no definition of a specific space for each artist, the exhibit will also present issues of authorization by blurring the collaborative and non-collaborative. Can two artist that work and live together ever make anything that is completely of that one individual?
Adam Lee Miller paints bold, restrained, hard-edge paintings of obstructions, cover-ups, holes and spills that may not be so accidental. While the work is largely hard-edged, Miller also welcomes chance through layering other techniques such as silkscreen, airbrush, faux boise, and stencil, in paintings that are purposely in conversation with the history of painting.
Nicola Kuperus shoots images that take on an almost performative quality, yet there is no performance. Kuperus also creates sculptural video works which reside inside peephole plywood boxes, inviting the viewer to submit to uncomfortable positions to experience the works through the strategically located openings.
Nicola Kuperus and Adam Lee Miller are both graduates from the College for Creative Studies, Detroit. They formed the band ADULT. in 1997. They have released 5 full length albums and toured the world multiple times, including Moscow, Bogotá and São Paulo. Between 2008 -2010 they (very) independently made a 100 minute film entitled "The Three Grace(s) Triptych" that has screened at the Anthology Film Archives (NY), the Detroit Institute of Arts, The Silent Movie Theater (LA), and film festivals in Mexico City, Chicago and Toronto. They have participated in two residencies together at SOMA Artist Residency (Mexico City) and C.A.J. Artist Residency (Saitama, Japan). They have exhibited work at Mattress Factory (Pittsburgh), Center Galleries (Detroit), and Dlectricity (Detroit). In 2013, they won a Knight Arts Challenge award entitled "Detroit House Guests" in which they turn their home into a artist residency for musicians where they collaborate on music for their next album. They currently have a video installation on exhibit at Lille3000 Renassaince (Lille, France). Individually, Adam Lee Miller has recently been included in several group exhibitions, including theCranbrook Art Museum (West Bloomfield, MI), Museum of Modern Art Saitama (Saitama, Japan), and Chicago Arts Coalition. Nicola Kuperus has recently exhibited at Center Gallery (Wolfsburg, Germany), Detroit Institute of Arts (Detroit, MI), and Centre d'Art Contemporain de Meymac (Meymac, France).
Simone Desousa Gallery


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Home Football Soccer News I am a less selfish player-Messi: Lionel Messi News
I am a less selfish player-Messi: Lionel Messi News
Football News: the Barcelona and Argentine star Lionel Messi believes that he is more of a playmaker kind of player rather than a Selfish goal scorer than he was in his earlier days.
Lionel Messi News: Lionel Messi acknowledges that now he is more of a team player than he was in his young days.
This season Lionel Messi is in peerless form for the Catalan giants, so fat he has scored 34 goals for them in all competitions. Three of that 34 goals he scored against Chelsea in the Champions League round of 16, where Barcelona thrashed their counterpart by 4-1 aggregate over the two legs.
@team @TeamMessi claims to be a more of a team player now. pic.twitter.com/cCMtmTXzwp
— Sportslibro (@Sportslibro) March 17, 2018
In the second leg at Camp Nou, Messi was in a splendid form; he has scored two goals for Barca and also made an assist for the French youngster Ousmane Dembele.
In an interview, the Argentine star says that he has given his attention to the other side of his game in the recent years.
Messi said: "Before, I tried to steal the ball and make my play,"
"Now, I try to make the team play more, pass the ball more and not be so defining or selfish.
"I try to move the team from another position. I run the same as I always did, but in a different way."
Now Barcelona will play against Athletico Bilbao on this Sunday in La Liga, and in the Champions League, they will face Serie A giants Roma.
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These films are very simply applied, according to the instructions printed on them, and remain in place for seven days. They can be worn in the shower, or removed for a bath and then replaced. They are very gentle for even the frailest skin and are certified non-toxic. They are especially recommended for the incontinent, who no longer have to wear two undergarments. Many people find them more comfortable and styling than pants with side pockets for hip protectors as they are more discreet and enable people to wear their own style of pants.
We supply them either with a pair of pads, or as a separate package of 16 pairs of films, intended to last 3 months.
These films are very simply applied, according to the instructions printed on them, and remain in place for seven days. They can be worn in the shower, or removed for a bath and then replaced. They are very gentle for even the frailest skin and are certified non-toxic. They are especially recommended for the incontinent, who no longer have to wear two undergarments. Many people find them more comfortable and stylish than pants with side pockets for hip protectors, as they are more discreet and enable people to wear their own style of pants.
We supply them either with a pair of pads, or as a separate package of 16 pairs of films, intended to last 2 months.


Soybean meal is an excellent and cost-effective protein source; however, its usage is limited in the piglet due to the presence of anti-nutritional factors and the antigens glycinin and β-conglycinin. The objective of the current study was to screen and select for bacteria that can be efficiently adopted to ferment soybean meal in order to solve this problem.
Bacteria were isolated from fermented soy foods and the grass carp intestine, and strains selected for high protease, cellulase and amylase activities. The isolated bacteria were characterized as Bacillus cereus, Bacillus subtilis and Bacilus amyloliquefacien, respectively. Fermentation with food-derived Isolate-2 and fish-derived F-9 increased crude protein content by 5.32% and 8.27%, respectively; improved the amino acid profile by increasing certain essential amino acids, broke down larger soy protein to 35 kDa and under, eliminated antigenicity against glycinin and β-conglycinin, and removed raffinose and stachyose in the soybean meal following a 24-h fermentation.
Our results suggest these two B. amyloliquefaciens bacteria can efficiently solid state ferment soybean meal and ultimately produce a more utilizable food source for growing healthy piglets.
Weaning is a critical period during swine production as sucking piglets are separated from the sow and transitioned to solid feed. The industrial prevalence of early weaning at 21–26 d of age resulted in reduced piglet performance including decreased feed intake, diarrhea, and retarded growth, due to many factors including an immature gastrointestinal system [1, 2]. Along with the underdeveloped immune function , separation stress  and dietary transition are required at the time of weaning. Due to the newly weaned pig facing these challenges their diets generally consist of highly digestible and palatable animal-based proteins such as: dried whey, spray dried plasma, poultry by-product meal and fish meal. There has been a push to try to limit these animal proteins due to possible risk of pathogen spread and rising costs. Soybean meal would be a more cost-effective alternative due to its high protein content, excellent amino acid profiles as well as the positive isoflavone properties [4–7].
Despite the high nutritional values soybean meal also has a few anti-nutritional factors, including glycinin and β-conglycinin, non-starch polysaccharides (NSP), raffinose family oligosaccharides (RFOs), trypsin inhibitors, and lectin [8–10]. Due to their underdeveloped digestive and immune functions young animals at weaning stage are less tolerant to these factors than more developed finisher pigs. Some heat labile factors such as trypsin inhibitors and lectin in the soybean can be deactivated by heat during feed processing [8, 9]. However, other factors, such as the antigenic proteins glycinin and β-conglycinin, can still have negative effects on the piglet. Glycinin has been well known to damage intestine morphology, interfere with the immune system, and increase diarrhea resulting in decreased growth performance in piglets [11–15]. β-conglycinin can elicit a hypersensitive immune response and negatively affect piglet growth performance [13, 16, 17]. Indigestible RFOs and NSP can result in discomfort in the gastrointestinal tract, changes in the digesta viscosity, and impede nutrient digestibility, ultimately negatively impacting growth performance in young piglets [10, 18, 19].
Microbial fermentation is an effective and cheap means to overcome the issues of soybean meal. Previous studies using molds, such as the Aspergillus species for soybean meal fermentation degraded the large antigenic proteins, increased the crude protein contents, and improved the non-essential amino acid profile [20, 21]. Feeding trials in piglets demonstrated the improved feed efficiency, amino acid digestibility and blood urea nitrogen when 10–15% fermented soybean meal was included in the diet . It has also been shown that the standardized ileal digestibility of amino acids in fermented soybean meal were similar to that of fish meal . These studies provide supports for using fermented soybean meal in the diet of early-weaned pigs. Besides the Aspergillus species, bacteria such as Bacillus and Lactobacillus species are also used for the fermentation of soybean meal . Compared with mold fermentation using bacteria could increase protein solubility, digestibility and the percentages of small-sized peptides in the soybean meal . The function of different types of microorganisms in the fermentation depends on their extracellular enzymes, including proteases, amylases, and α-galactosidases , and therefore the choice of the organism for fermentation may have profound effects on the nutritional quality of the final fermented soybean meal.
Traditional Chinese fermented soy foods undergo natural spontaneous fermentation  and contain various bacteria with high enzyme activities. The primarily herbivorous grass carp feeds on aquatic plants and has been found to contain bacteria with cellulolytic properties [27–30]. The fermented soy products and the grass carp are two desirable sources for isolating generally recognized as safe bacteria with high protease and cellulolytic properties in order to degrade soy antigens and structural carbohydrates of the soybean meal. The current study aimed to identify new food- and intestine-derived bacteria which are good candidates for soybean meal fermentation to improve the nutrition values.
Luria Bertani (LB), Bacto Brain Heart Infusion (BHI), and DeMan, Rogosa and Sharpe (MRS) agar plates were used to isolate bacteria colonies. LB agar (pH 7.5) were prepared with 1.0 g of tryptone powder (Bio Basics Canada INC, Canada), 0.5 g of yeast extract (Bio Basics Canada INC, Canada), 1.0 g of NaCl (Fisher Scientific, USA) and 1.5 g of BactoAgar in 100 mL deionized water. BHI (Becton, Dickinson & Company, USA) and MRS (Oxoid, England) agar plates were prepared according to manufacturer's procedures with the addition of 1% (w/v) BactoAgar.
Soy milk, carboxymethyl cellulose (CMC), starch agar plates were used to select colonies with proteolytic, cellulolytic and amylase activities, respectively. Soy milk agar was made by mixing 1 g of soy milk powder (Bulk Barn, Canada) and 2.5 g of BactoAgar (Becton, Dickinson & Company, USA) with 100 mL of water as described by Amoa-Awua et al. . CMC agar plates were prepared by adding 1.0 g of CMC (Acros Organics, USA) and 1.5 g of BactoAgar to 100 mL of deionized water as previously described by Sazci et al. . To prepare starch agar plates, 1 g of corn starch (Bulk Barn, Canada) and 1.5 g of BactoAgar were added to 100 mL of deionized. All plates were autoclaved for 25 min at 121 °C.
Five types of fermented soy products, including black fermented beans, red fermented beans, yellow fermented beans, soft fermented beans and Japanese Natto, were obtained from the local supermarket and used to isolate proteolytic bacteria. Approximately 1 cm3 of each source of fermented products was mixed with 500 μL of sterile water and serial dilutions were conducted to isolate approximately 100 clearly defined individual colonies of bacteria per plate. A hundred microliter of diluted samples from each of the five sources was plated onto the soymilk agar and incubated for 24 h in 37 °C. For each food source, colonies demonstrating the largest clear zone were selected and glycerol stocks were created for storage of isolates at − 80 °C. A total of six isolates were subjected to further screening for cellulase and amylase activity.
Contents from the proximal, middle and distal section of grass carp intestine were harvested to isolate bacteria with cellulolytic activity . Diluted samples were first plated onto LB, BHI and MRS agar. Any colony present on the three plates was blotted onto the soymilk and CMC plates and incubated at 37 °C for 72 h. The clearance zone in the CMC agar plates was revealed by Congo Red staining as described [32, 33]. Colonies which were able to grow on the soymilk agar and exhibited the highest cellulase activity were selected and a total of three colonies were selected and further screened for enzymatic activities.
A single colony of each selected isolate was inoculated into 1 mL of BHI culture media and incubated for 18 h at 37 °C to extract crude enzymes . The supernatant was harvested by centrifuging the liquid culture at 10,000 r/min for 15 min at 4 °C using an Eppendorf 5415D centrifuge. Twenty microliters of each crude enzyme extract were pipetted into the punctured hole on the soy milk, CMC and starch agar plates, respectively, and incubated at 37 °C for 72 h. Enzymatic activities were ranked by the clearance zone measurements taken from the edge of the colony (soy milk agar plate) or the edge of punctured hole (CMC and starch agar plates) to the outer edge of the clearance zone. The amylase clearance zone was revealed using an additional Gram's Iodine Stain . Each experiment was repeated three times using fresh inoculums each time. Commercial B. subtilis ATCC 6633 with protease , amylase  and cellulase activities  was used as the positive control and the blank culture media was used as the negative control.
All selected bacteria selected were characterized to the species or genus level by matrix assisted laser desorption ionization- time of flight mass spectrometry (MALDI-TOF MS). Isolates were individually streaked onto the BHI agar plate, and incubated for 18 h at 37 °C. Each fresh cultured bacterial colony was spotted onto the target plate (Bruker), air dried, and overlaid with 1 μL matrix solution mixed with an organic solvent solution made of 50% acetonitrile (Sigma Aldrich, Canada) and 2.5% trifluoroacetic acid (VWR, Canada). The target plate was then placed onto the MALDI-TOF MS instrument (Bruker) for analysis. Peptide mass fingerprint spectra was scored using MALDI-TOF MS software and database (MALDI Biotyper 3.0, Bruker). An isolate was automatically identified to the species level if the score was 2.0 to 3.0, or to genus level if the score was 1.7 to 1.999. No reliable identification was generated if the score was less than 1.7.
The 16S rRNA and gyrB genes were used to further identify the strain of bacteria isolates. Genomic DNA of each isolate was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen, USA) according to manufacturers' instructions. The partial 16S rRNA gene was amplified and sequenced using the same primer set of BSF8/20 and BSR534/18  and the gyrB gene was amplified by the universal primer set of UP-1 and UP-2r and sequenced by the primer set of UP1S and UP2SR (Table 1) . Before DNA sequencing using ABI Prism 3100 Automated Sequencer, the size of each PCR product was verified by agarose gel electrophoresis and the concentration of the PCR products was determined using a BioPhotometer Plus spectrometer (Eppendorf, USA) to ensure that the minimum concentration required. Bacterial strains were identified by blasting the sequencing results from both genes against nucleotide sequences in the GeneBank database (http://blast.ncbi.nlm.nih.gov/Blast.cgi).
Soybean meal (48% crude protein content, Grand Valley Fortifiers, Canada) was sterilized by mild heating at 125 °C for 30 min to minimize lysine loss . For each fermentation, bacteria inoculums of selected isolated were prepared fresh by incubating a single colony in the BHI culture media for 18 h at 37 °C to obtain a culture of 108 colonies forming unit (CFU)/mL. Solid state fermentation was set up by inoculating 2 × 108 CFU/mL isolated bacteria into the 2 g of soybean meal for each of the treatment group with a volume: weight ratio of 2:1. This mixture was set to ferment in an incubator at 42 °C for 48 h without any agitation. Each fermentation was performed in triplicate. Samples were collected at 24 and 48 h and stored at − 80 °C. Lyophilized samples were then ground into a fine powder for further analyses.
Total soluble proteins of the unfermented and fermented soybean meals were isolated as previously described by Hong et al. . The quantification was carried out using the DC Assay Kit (Bio-Rad Laboratories, USA). Fractions of soluble protein extract were analyzed by 11% tris sodium–dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A volume of 15 μL of denatured total soluble protein sample was loaded to the gel, and subjected to electrophoresis for 100 min using 100 V. The gel was stained with Coomassie blue. Images were taken using ChemiDoc XRS+ (Bio-Rad Laboratories, USA) and analyzed with the Image Lab software.
To determine piglets' immunoreactivity against an unfermented and fermented soybean meal, Western blots were performed. Total soluble protein of 50 μg each was loaded into an 11% SDS-PAGE as described above and then transferred to an Immobolin-P (Millipore, Billerica, USA) polyvinyl difluoride transfer membrane using the wet transfer method at 100 V for 100 min. The membrane was first blocked with buffer comprised of 5% skim milk, 0.1% Tween 20, and 1× TBS for 1 h before the primary antibody incubation. After several washes with washing buffer (0.1% Tween and TBS), the membrane was incubated with the primary antibody at 4 °C overnight. The primary antibody was prepared against soybean antigenic proteins glycinin and β-conglycinin following a published protocol . Briefly, plasma was collected from 8-week-old piglets that were previously exposed to feed containing soybean meal for 21 d and developed an immune response to soybean allergens. The primary antibody was comprised of 20 μL of pooled pig serum and diluted to 1:500 with the blocking buffer. Rabbit anti-pig horseradish peroxidase-linked IgE was used as the secondary antibody (1: 100,000; Abcam). Proteins were detected by using Clarity Western Enhanced Chemiluminescence substrate (Bio-Rad Laboratories, USA) according to manufacturer's instructions.
Lyophilized samples of unfermented and fermented soybean meal were subjected to the measurement of crude protein and amino acid profiles according to the corresponding AOAC official methods (moisture, method 934.01; crude protein, method 954.01; amino acids, method 994.12). Raffinose and stachyose were determined using high-performance liquid chromatography (HPLC, Shimadzu LC-15C, Japan) equipped with a differential detector (Shimadzu RID-10A, Japan) as described by Yao et al. . Briefly, stachyose and raffinose were extracted by 70% (v/v) ethanol with the assistance of microwave. The supernatant was concentrated and reconstituted to 25 mL. A volume of 20 μL of each sample was injected into the HPLC system with 70% acetonitrile as the mobile phase. The temperature was set at 35 °C, and the flow rate was controlled at 1 mL/min. Serial diluted standards of stachyose (Dr. Ehrenstorfer, Germany) and raffinose (Dr. Ehrenstorfer, Germany) were used to determine the stachyose and raffinose concentrations.
All statistical analyses were performed using PRISM GraphPad Prism Version 5.03 (San Diego, CA). The data for bacteria screening were analyzed using one-way ANOVA with a Dunnet post-test to determine if any treatment group was significantly different from the positive control. Comparisons in the proximate analysis, amino acid profile and oligosaccharide concentrations of the unfermented and fermented samples were performed using one-way ANOVA followed by a Tukey post-test; observations were made in triplicate with one observation obtained daily. The assumption of no day effect was believed to be true. Data was expressed as mean ± SEM and considered to be significant if P < 0.05.
All bacteria isolates demonstrated protease activity on the soymilk agar plates and the isolate having the largest clearance zone was Isolate-4 from food origin and Isolate 11 from fish intestine. Protease activity of Isolate-4 and F-11 was greater than that of B. subtilis ATCC6633 (positive control; P < 0.05; Fig. 1a). All three isolates derived from fish intestine were tested positive for cellulase activity and only Isolate-2, Isolate-7 and Isolate-8 of food origin also demonstrated cellulase activity (Fig. 1b). Of all the isolates tested only F-8 had a greater cellulase activity (P < 0.05; Fig. 1d) when compared to the positive control (Fig. 1c). Food-origin isolates, Isolate-2, Isolate-4, Isolate-7 and Isolate-8, and the three fish-origin bacteria also tested positive for amylase activity. The Isolate-2 and Isolate-8 demonstrated significantly higher amylase activity (P < 0.05; Fig. 1f) than that of the positive control (P < 0.05; Fig. 1e). A summary of the enzymatic activities of all bacteria isolated are shown in Table 2. A total of six isolates (Isolate-2, Isolate-7, Isolate-8, F-8, F-9 and F-11) were shown to have protease, cellulase and amylase activities and were further included in the solid-state fermentation analysis.
Isolates with tested enzymatic activities were identified as Bacillus using the MALDI-TOF MS (Table 2). Isolate-1 from black fermented beans and Isolate-5 from soft fermented beans were identified as B. cereus, and Isolate-7 and Isolate-8 from Japanese Natto were B. subtilis. Isolate-2 from red fermented beans, Isolate-4 from yellow fermented beans as well as F8, F9 and F11 of the fish-origin were unable to be identified to species level but gram staining identified the bacteria as gram positive bacilli.
By sequencing the partial 16S rRNA and gyrB genes, Isolate-2, Isolate-4, F-9, and F-11 were identified as B. amyloliquefaciens. The gyrB gene sequences of Isolate-2, Isolate-4, F-9 and F-11 had an identity of 98%, 95%, 98% and 97% with B. amyloliquefaciens subsp. plantarum strain FZB42 (GeneBank accession # NC_009725.1), respectively. The partial 16S rRNA gene of Isolate-2 and F-9 had a 99% identity with B. amyloliquefaciens strain IHB B 3373 (GeneBank accession # KF475869.1). The partial 16S rRNA sequences of Isolate-4 were 99% identical to B. amyloliquefaciens strain BAB-68 16S (GeneBank accession # KC250107.1) and F-11 shared 99% identity with B. amyloliquefaciens subsp. Plantarum UCMB5033 (GeneBank accession # HG328253.1). The F-8 was identified as B. subtilis. Its partial 16S rRNA gene sequence had a 99% identity to B. subtilis strain IHB B 4270 (GeneBank accession # KF475879.1) and its gyrB gene sequence had a 97% identity to B. subtilis subsp. subtilis str. 168 (GeneBank accession # NC_000964.3).
Total soluble protein concentrations in the fermented soybean meal were significantly increased by Isolate-2 and F-8 after 24 h solid-state fermentation and increased by Isolate-7 and F-9 after 48 h treatments when compared to the unfermented SBM control (Fig. 2). To analyze the influence of fermentation on the soybean meal protein profile, sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed. The maximal degradation of large proteins in the soybean meal was almost complete at 24 h of fermentation (Fig. 3), however, the distributional pattern was different between food-origin and fish-origin isolates. As shown in Fig. 3, the fermentations using both the food and fish intestine sourced bacteria were successful in reducing the antigenic protein β-conglycinin including the α'-subunit (76 kDa), α-subunit (72 kDa) and β-subunit (53 kDa). The food sourced bacteria (Fig. 3, lanes 2–9) fermentation can degrade most of the soybean meal soluble proteins to 25 kDa (Fig. 3a), which was approximately 10 kDa smaller than the proteins fermented by bacteria of fish origin (Fig. 3b; lanes 2–7).
Western blot analysis using the serum of soybean meal-challenged piglets as the primary antibody demonstrated immunoreactivities at approximately 75 kDa, 40–50 kDa, and 30–40 kDa for the unfermented soybean meal (Fig. 4). At 24 h of fermentation, immunoreactivities were abolished in fermented soybean meals treated with Isolate-2 and F-9 (Fig. 4a, lanes 3, 6) although reduced immunoreactivity was still present in the soybean meals treated with Isolate-4, F8 or F11 (Fig. 4a, lanes 4, 5, and 7, vs. unfermented soybean meal in lane 2). By 48 h, no immunoreactivity was visible in all fermented products (Fig. 4b).
Crude protein analysis showed that solid-state fermentation significantly increased crude protein concentrations from 48.05 ± 0.47% to 53.37 ± 0.67% with Isolate-2 or 56.32 ± 0.73% with F-9 at 24 h, respectively. As shown in Table 2, the total amino acids as well as essential (EAAs) and nonessential amino acids (NEAAs) were also increased after 24 h-fermentation with Isolate-2 and F-9. The soybean meal fermented with Isolate-2 was greater in lysine, valine, histidine, phenylalanine, cysteine, proline, tyrosine, asparagine, glutamine and glycine when compared to the unfermented control. With F-9, lysine, valine, histidine, phenylalanine, cysteine, proline, tyrosine, asparagine, glutamine and glycine were increased, but sulfur-containing amino acids methionine and cysteine were decreased.
The unfermented soybean meal contained 9.88 ± 0.20 mg/g raffinose and 41.73 ± 1.70 mg/g stachyose; at 24 h of fermentation, both oligosaccharides levels were below the detection levels in the soybean meal.
From fermented soy foods and the grass carp intestine six isolates of Bacillus species were identified to have protease, cellulase and amylase activities. It was consistent with previous studies that the isolated bacteria from other traditionally fermented foods, such as Dawadawa , Meju , Cheonggukjang , and Douchi , were Bacillus species. These bacteria can survive on legumes because they secrete extracellular enzymes, such as proteases . Among the isolates, Isolate-2 of food-origin and F-9 of fish-origin were identified as the most efficient isolates, which eliminated most of the antigenicity againstβ-conglycinin and glycinin and achieved the maximal soluble protein fractions, crude protein as well as amino acids profiles at 24 h.
Isolate-2 and F-9 were further identified as B. amyloliquefaciens by sequencing 16S rRNA and gyrB genes. The gyrB gene is responsible for producing the β subunit protein of a DNA gyrase and is essential in DNA replication . This gene is distributed universally among species and has a faster rate of molecular evolution than the 16S rRNA gene and therefore is more accurate in identification of bacteria strains . In general, the results using the 16S rRNA gene were consistent with the gyrB gene. One of the isolates F-11 was identified as B. subtilis using the partial 16S rRNA while it was identified as B. amyloliquefaciens using the gyrB gene. B. amyloloiquefaciens has been less investigated in fermentation of soybean compared to B. subtilis. A recent study has used a B. amyloliquefaciens isolated from the Korean traditional soybean paste in the soybean meal fermentation and found it significantly improved the nutritional quality and bioactivity .
Solid-state fermentation was used in the present study because this application is more desirable to the agricultural industry setting due to less costly equipment and lower moisture levels in the liquid fermentation [47, 48]. Our results showed that solid-state fermentation using Isolate-2 and F-9 could degrade large proteins to less than 25 kDa or 35 kDa, respectively, and antigenicity against β-conglycinin and glycinin were completely eliminated in the soybean meal by 48 h of fermentation. A previous study using B. amyloliquefaciens isolated from soybean paste also demonstrated a reduction in proteins size to 27 kDa and under . The reduction of protein sizes was important to increase the digestibility of soybean proteins . Moreover, removal of these antigenic soybean proteins is beneficial for both digestion and animal health. Antigenic proteins found in soybean meal could activate the immune system leading to inflammation of the intestine and the release of compounds such as histamine, which increases the incidence of diarrhea [13, 16]. It is interesting that the degrading effect of food-origin B. amyloliquefaciens was quite similar, but displayed a distinct digested soybean meal protein profile when compared with that of the fish-origin bacteria isolates. It is possible that different species and subspecies of bacteria may secrete distinct arrays of proteinases that target different subsets of the soybean proteins.
Fermentations were also able to decrease the oligosaccharides and RFOs to non-detectable levels at 24 h. Previous studies have reported a decrease in the soy oligosaccharides, stachyose and raffinose, and NSPs when soybean meal was fermented with bacteria , although a much longer fermentation (10 d) was required. The reduction of these oligosaccharides could have been due to hydrolysis of the α-1,6-galactose linkage by the enzyme α-1,6-galactosidase. Some strains of Bacillus have been found to secrete extracellular α-1,6-galactose  which may explain the reduction of oligosaccharides observed. Further characterization of the extracellular enzymes secreted by Isolate-2 and F-9 should be investigated to support the observation.
Fermentation could substantially increase the crude protein contents of the soybean meal , however, the efficacy could vary among different microorganisms. In the present study, the crude protein was significantly increased by 5.32% and 8.27% following 24 h of fermentation with the two B. amyloliquefaciens isolated from fermented food and fish intestine, respectively. The fermentation with B. amyloliquefaciens derived from soybean paste was found to increase the crude protein content by 6.42% . In the same study, fermentation with two Lactobacillus spp. and Saccharomyces cerevisiae was observed to increase the crude protein by 1.05%, 1.91% and 5.61%. Soybean meal fermented with Aspergillus oryzae has been reported to have a crude protein increase ranging from 1.95%  to 10% . The increase in crude protein may be explained by two processes. Firstly, the increased crude protein observed could be due in part to the decrease in carbohydrates . Some bacteria can break down cellulose, polysaccharides, and oligosaccharides and utilize its sugar subunits for their metabolism and respiration processes .
In addition to the crude protein, the amino acids profiles of the fermented soybean meal were improved with Isolate-2 and F-9. The total amino acids were increased by 13% and 15% with the amino acid profiles sharing a similar pattern between the soybean meals treated with Isolate-2 and F-9, respectively. The EAAs, including lysine, valine, histidine and phenylalanine were increased in the fermented soybean meal from a range of 15–19%. There existed differences in amino acids between the soybean meal fermented with Isolate-2 and that fermented with F-9. The proline content was greater while the methionine and cysteine amino acids being lower in samples treated with F-9 when compared to Isolate-2 and the unfermented control. The alterations in amino acid profiles had a great relationship with the microorganism used. Methionine rather than lysine was increased in the soybean meal fermented with B. amyloliquefaciens . Hong et al. observed a significant increase in glycine, glutamic acid, and aspartic acid when soybean meal was fermented with A. oryzae . Using cracked soybean fermented with A. oryzae it was reported that there was a significant increase in all amino acids except arginine and lysine ; by contrast, cracked soybeans fermented with B. subtilis increased aspartic acid, glutamic acid, serine, alanine, proline, valine, methionine, cysteine, isoleucine, phenylalanine, tyrosine and lysine levels after 48 h . The current study, unlike some of the previous ones was able to increase lysine and threonine, two limiting amino acids of the corn-soybean meal for swine, which could decrease the requirement of synthetic amino acids to be added.
Fermented soybean meal has also been shown to improve animal performance. A previous study performed using the spores of B. amyloliquefaciens to ferment whole soybean as a target dietary supplement for use in poultry feeds was able to increase growth rate and feed efficiency by 8% and 8.8%, respectively . Also, this species of bacteria has been found to have anti-bacteria activity  and antioxidative activity [46, 55], which may be able to improve growth performance for weaned piglets. The effect of replacing some soybean meal using soybean meal fermented with the two B. amyloliquefaciens strains isolated in the current study on animal production will be investigated in future studies.
In conclusion, fermentation using the newly isolated food-derived Isolate-2 and fish-derived F-9 resulted in an improved protein profile, an increase in crude protein concentration, an increase in total amino acid concentration, and a decrease in raffinose and stachyose in the soybean meal. These two natural derived bacteria have the advantages of being safe, time-efficient and good at producing a more balanced amino acid profile. They could be good seeding bacteria in the solid state fermentation of soybean meal with the goal of improving the health of weaned piglets.
Kees DeLange is deceased. This paper is dedicated to his memory.
The authors thank Zeming Ding for analyzing amino acids.
Swine Innovation Porc Canada provided the funding support, but did not involve in experiment and data interpretation.
SM conducted bacteria isolation, SBM fermentation and protein separation experiment, co-drafted the manuscript; JX conducted amino acid and raffinose and stachyose analysis, co-drafted the manuscript; PWD conducted PCR amplification on the 16S RNA and gyrB gene; HYC supervised the bacteria characterization and identification using MALDI-TOF MS; KD discussed the project; HZ contributed to the experimental design and result discussions; JL oversaw the design and development of the project, data interpretation, and manuscript revision. All authors read and approve the final manuscript.


When content from a News Page is posted to Facebook or another social media platform, certain other pieces of information from the page beyond the content text will be automatically pulled into Facebook as well.
There are several standard pieces of information that go along with a News Post when it's shared on social media. In order to standardize what metadata accompanies a post, Facebook created and shared a standard called "Open Graph." This means that any social platform that takes advantage of Open Graph will display metadata like an article's title or thumbnail image consistently.
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Note that these Open Graph tags are only generated when content is shared from a News Page - a Composer page with a News Post element which has the "Override page's title and metadata" option selected.
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IRVINE, Calif. — CarFinance Capital LLC announced that it has secured a $200 million warehouse credit facility from Deutsche Bank and Credit Suisse.
Launched in May 2011, CarFinance Capital currently serves over 1,000 dealers nationwide with indirect, less-than-prime lending solutions. The finance source also offers first-look opportunity on referrals from the company's consumer direct lending site, CarFinance.com.
CarFinance Capital's indirect lending product offers credit for the mid-market nonprime spectrum (from 525 FICO through 675), dealer flexibility and negotiating room, rapid funding and reimbursement, and one-on-one customer support.
The company's dealer referral program sends approved and financed customers from CarFinance.com to participating dealers to make their vehicle purchases. The program is a paperless, online application process that can minimize a dealer's work, as all required stipulations have been collected and the signed agreement is in hand.
CarFinance Capital's solution funds dealers' bank accounts directly via ACH, so, when paid, the funds are available immediately. The program provides loan-to-value ratios of up to 120 percent and $2,000 for back-end products sold by the dealer.
The company is licensed in more than 24 states for both its direct and indirect businesses and will roll out nationwide in the coming months, according to CarFinance Capital. For more information, visit www.CarFinanceCapital.com.


text:
NBA Finals: Warriors need someone other than Curry
Celtics, Curry, Finals, jayson tatum, nba, Warriors
Jakarta (ANTARA) – If you look at the first three games of the NBA Finals this season, it's hard to deny Stephen Curry's big role as the lifeblood of the Golden State Warriors.
Among all the performers, including the Boston Celtics players, Curry was the most prolific figure in the three games of the 2022 NBA Finals. On average, Curry contributed 31.3 points after 34 points in the first game, 29 points in the second game and 31 points in the second game. third game.
In the final minutes of the third game yesterday, Curry suffered an injury and was out for a while before being able to force himself to continue the game until he was withdrawn by head coach Steve Kerr who felt that the 14-point gap from the Celtics would not be able to be caught by the Warriors with two minutes remaining.
Curry claims that he is fine and can still be on the floor for the fourth game which was played at TD Garden, Massachusetts, Friday local time (Saturday WIB).
The Warriors showed similar optimism, especially when Curry's condition did not require an MRI scan and the star continued to take part in Thursday's training session (9/6).
"I'm going to play. That's all I know right now," Curry said at his final press conference ahead of the fourth game broadcast on the NBA's YouTube channel on Friday.
Although Curry is optimistic, so are the Warriors, but of course the 34-year-old basketball team is faced with a big task to take on a more significant role in the fourth game later.
Boston Celtics guard Marcus Smart (left) closely monitors the movement of Golden State Warriors star Stephen Curry in the third game of the NBA Finals at TD Garden, Massachusetts, United States, Wednesday (8/6/2022) local time. (ANTARA/REUTERS/USA TODAY SPORTS/Kyle Terada)
Klay Thompson is of course the first name that comes to mind when talking about someone other than Curry who should be able to play a bigger role ahead of the fourth game.
If in the Western Conference finals Thompson delivered "Game 6 Klay" performance one game earlier, perhaps the same thing happened two games earlier in the NBA finals.
The third game yesterday was a sign that Thompson had started to show positive progress by packing 25 points to improve his average contribution throughout the NBA finals to 17 points.
In the third game Thompson was also much more aggressive in shooting than the first game, but his accuracy and conversion rate improved compared to the second game.
Jordan Poole who stole the show with a shot buzzer-beater In the third quarter, the second game is also expected to play a more significant role.
Besides Thompson and Poole, Andrew Wiggins may also be another name that is expected to be more significant in supplying the numbers for the Warriors in the fourth game after having an average contribution of 16.3 points.
If Thompson and Poole are expected to play a role, Wiggins is certainly expected to be able to help the Warriors speak more significantly in the paint area or the area around the basket which must be admitted to be the key in determining victory in the previous two final games.
So it wasn't just Wiggins, Kevon Looney, who had set a record as the first Warriors center since Robert Parish in 1977 to score 20 points and 10 rebounds in a playoff game in Game 2 of the Western Conference finals against the Dallas Mavericks.
Another option that might be a new solution is to give more playing minutes for debutant forward Jonathan Kuminga who has a promising posture in the battle in the paint area.
"You can't rule anything out. It's something we continue to discuss among the coaching staff every day. Do we need to put another player in the rotation? Do we need to change the combination?" Kerr said.
Whoever it is the name that will appear to take on a more significant role or again rely on Curry, the Warriors who are currently trailing 1-2 to the Celtics will clearly treat the fourth game with a must-win situation if they don't want to be on edge when they return to San Francisco to Game 5 later.
Golden State Warriors star Stephen Curry (C) is seen wincing in pain after sustaining an injury in the middle of a wild ball struggle in the third game of the NBA Finals against the Boston Celtics at TD Garden, Massachusetts, United States, Wednesday (06/08/2022) local time. (ANTARA/REUTERS/USA TODAY SPORTS/Winslow Townson)
Scramble for paint area
Except for the first game when the Celtics orchestrated their brilliant comeback supported by six points in a 20-2 streak, the last two games have always shown the team that dominates the paint area will come out victorious.
In fact, in the first game the Celtics, who won, also outperformed the Warriors 34-26 in the paint area, but it's hard to say that the paint area played a more significant role than their tripoin accuracy, which reached 51.2 percent, the highest in the series so far.
The second and third games present a battle between two teams who are relatively equally good at executing shots outside the arc, but the points obtained in the paint area are clearly the main differentiator.
In the second game, the Warriors, who outperformed the paint area 40-24, were able to walk smoothly to win 107-88 over the Celtics.
The Celtics changed full control of the paint area in the third game with a superior score of 52-26 to beat the Warriors 116-100.
The dominance of the Celtics paint area was also continued by how they mastered the rebound aspect, namely 47-31 compared to the Warriors, including the success of securing 40.8 percent of the situation. offensive rebound which translates further through the conversion of 22 to 11 second chance points.
Another important aspect that can be read from the battle for the paint area is how both teams protect their respective defensive areas and in fact in the second game the Warriors have to admit failure for allowing the Celtics to bring the ball through the opponent's defense (drive) 57 times, or 22 times more. versus 35 drives the Warriors did alone.
The Warriors' weakness in the paint area of the second game of course cannot be separated from the less than perfect appearance shown by the aging forward Draymond Green.
Green had just two points, four rebounds and three assists over 35 minutes before committing a sixth foul or being charged foul out approximately four minutes before the dispersal of the fourth quarter.
The figures will certainly invite criticism, but Green's colleagues still believe the 32-year-old will soon find his big role again.
"Yeah, there's definitely a reason Draymond is here. We wouldn't be the Warriors without Draymond. He's one of the best players I've ever shared the field with. We're all human, we can't be immune to a bad night or two," said Thompson.
"I know Draymond will return to his signature game that is full of physical contact, is fast, loud and becomes the 'Money Green' that Dub Nation loves," he added.
Boston Celtics basketball player Jayson Tatum (0) tries to enter the ball while being escorted by Golden State Warriors basketball player Stephen Curry (30) in the third match of the 2022 NBA Finals at TD Garden, Boston, Massachusetts, USA (8/6/2022). The Boston Celtics won 116-100 over the Golden State Warriors to temporarily lead the score 2-1 in the NBA Finals. BETWEEN PHOTOS/Paul Rutherford-USA TODAY via Reuters/aww.
Green really needs to rediscover his best form and work hand in hand with Wiggins and Looney – Kuminga if necessary – to win the paint area both defensively and attacking.
Third quarter puzzle
The Warriors' skill in controlling the third quarter and the important impact that can be generated from it has been repeatedly expressed and acknowledged by the players and head coach of the Celtics.
However, until the three NBA finals have been running, the Celtics have not been able to overcome this problem.
Of course, even though in three games they became the Warriors' months in the third quarter, the Celtics still managed to win both of them.
And in the third game there was positive progress shown in the Celtics' efforts to stem the Warriors' explosive power in the third quarter, when they only trailed by 25-33 points compared to 24-38 in the first game or 14-35 in the second game.
Celtics guard Marcus Smart even admitted that he couldn't explain why his team overall lost 43 points in the third quarter of the three final games so far, even though in the first, second and fourth quarters they could have beaten the Warriors 52 points in total.
"I don't know. That seems like something that's going to be a mystery. In the end we just got to the floor and tried to do our best," said Smart.
"You can plan and in fact so far it's not working…. Of course we don't want this to be a sustainable trend," he added.
Not only was Smart confused to explain, Kerr also didn't know why the Warriors could be so bright in the third quarter.
Golden State Warriors guard Stephen Curry (30) fights for a rebound with Boston Celtics center Al Horford (42) and guard Derrick White (9) in the second game of the 2022 NBA Finals at Chase Center, San Francisco, California, Sunday (5/6/2022) ). ANTARA/REUTERS/Ezra Shaw/pool photo-USA TODAY Sports
"I usually open a book filled with aphorisms from movies or history, pick the right one and fuel the spirits of the players, then suddenly they play better," he joked.
"I don't know. It's been our trend for years, and it doesn't seem to have anything to do with what I or the other coaching staff say. It's in the hands of the players, they just do it," Kerr added.
Although the Warriors' skill in the third quarter may return to be an unsolved puzzle, of course they hope it will help pave the way for winning the fourth game.
On the other hand, the Celtics hope that even if the Warriors beat the points again in the third quarter, as long as they bounce back in the fourth quarter to win, that would be enough.
#NBA #Finals #Warriors #Curry
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Elizabeth Banks brings her girl-next-door looks and approachable acting style to even the smallest of roles. She�s had bit parts in Spider-man and Catch Me If You Can, but really shone as Marcela Howard in Seabiscuit. Her resume grows with The Baxter, The 40-Year-Old Virgin and Slither.


text:
alter table NOTIFICATION_HOME
 add ("DATAVERSION" integer default 0 not null);

alter table NOTIFICATION
  rename column XML_TYPE to NOTIFICATION_TYPE;
alter table NOTIFICATION
  rename column XML_DATA to NOTIFICATION_DATA;

  -- Sharing enumeration updates
insert into CALENDAR_BIND_MODE (DESCRIPTION, ID) values ('indirect', 4);

insert into CALENDAR_BIND_STATUS (DESCRIPTION, ID) values ('deleted', 4);

-- Now update the version
update CALENDARSERVER set VALUE = '30' where NAME = 'VERSION';
insert into CALENDARSERVER (NAME, VALUE) values ('NOTIFICATION-DATAVERSION', '1');

redpajama_set_name: RedPajamaGithub


HOPE – The Hope School Board tackled a full plate of items Monday night as it unanimously extended the contract of HPSD Superintendent Bobby Hart and addressed two new academic initiatives, as well as a three-phase building plan for the expansion of Beryl Henry Elementary School.
Hart's contract was extended by the board for one year. He came to the HPSD as superintendent in April, 2012, from the Genoa Central district near Texarkana. A native of Bryant, Hart graduated from Henderson State University.
The contract extension comes on the heels of an evaluation process completed by the board in January.
Hart announced one new academic initiative Monday night and the board acted on another, as he briefed the board on an early childhood literacy initiative and asked the board to authorize a principal's position for the new Hope Academy of Public Service.
He said the early childhood initiative is in its organizational stages.
"We are starting to reach out to people and develop our plan," Hart said.
Hart said currently 80 percent of HPSD kindergarten students are ready for first grade, for which he credited teacher engagement.
Also Monday night, Hart received approval from the board to begin advertising for a principal for the grades 5-8 Hope Academy of Public Service. He said public forums conducted recently for parents at Beryl Henry Elementary School and Yerger Middle School were well attended and the concept well-received.
The school will be housed on the former Garland School campus, and will be a public service-centered academy that will provide a rigorous curriculum to prepare its students as college-ready scholars through collaborative and individual learning and computer-centered instruction in conjunction with the William J. Clinton School of Public Service in Little Rock, the University of Arkansas Hope-Texarkana, and other institutions of higher education.
Hart said the district will accept applications from within and outside of the district for the position. The Hope APS is scheduled to begin open enrollment Feb. 22 through April 4, and classes are to begin in August, 2016.
He said a parental advisory committee for the Hope APS will have its organizational meeting Tuesday night at 6 p.m. in the school administration building conference room. The meeting is open to the public, Hart said.
The board also learned Monday night that architectural plans for the BHE expansion and removal of 30-year old metal buildings from the campus have been set. Hart told the board that architects envision a three-phased approach: first, the renovation of the front of the existing building to provide for conversion to office space; secondly, the construction of new classrooms and activity area; and, third, demolition work and removal of existing metal buildings.
Completion of the project is expected to take up to a year.
Also Monday, the board resolved its questions concerning a proposed contract for rehabilitation of the Hope High School track, and confirmed the award of the work to the only bidder for the project, Tri-State Track, at a cost of $94,800. Hart said the company will issue a six-year warranty for the work.
--Recognized art students from Hope High School for their creation of a mural for the entrance to the Alternative Learning Center at HHS.
--Heard a report from Assistant Superintendent for Transportation and Facilities Steve Bradshaw concerning the success of the district's recent surplus property sale, which netted some $19,384.40.
--Heard a report from Hope High School Principal Tommie Campbell regarding a revamping of scheduling at HHS.
--Received a brief tutorial from HPSD Technology Coordinator Jeff Madlock regarding a new mobile phone application which allows users to access websites for each school campus and the district to obtain up-to-the-minute notification and information.
--Adopted on first reading a series of Arkansas School Board Association model policies.
--Tabled a proposed bid award for grounds maintenance services.
--Revised bus driver hourly route and field trip pay rates.
--Approved facilities rental requests for the 21st Century Learning Program fashion show, a spring pageant, and a dance on the Hope High School campus; but, denied a request from a semi-professional football league for use of Hammons Stadium for lack of liability insurance.
--Accepted classified and certified resignations and retirements, including the retirement of Yerger Middle School Principal Vanessa McCraw.
--Rescheduled its March meeting to March 28 because of spring break.


We praise God for who He is … for His love, his mercy, his majesty. We give thanks for the life and death of Jesus Christ that washes away our sins.
We pray for the lost and hurting in the remote corners of the world. We lift up our co-laborers in Christ—the many churches, missionaries, and aid workers MAF serves. We pray for peace, for a cessation of hostilities, for a halt to the hurt and ugliness so prevalent in the kingdoms of men.
We lift up each other, praying for MAF missionaries around the world. We pray for safety, for joy in our work, and for effectiveness in our ministry. We pray that our missionary children (MKs) will grow into Godly men and women who will seek His will in all they do.
We pray to the Lord of the Harvest, that He will send more workers into the field.
And we pray for you, the friends and supporters of MAF. May God bless you for your faithfulness and give you a heart to serve. May you be filled with the peace that passes all understanding.
Will you please join us in prayer on Wednesday, October 23?