Three kaiten sushi chains have managed to bring their prices down to ¥100 per plate, which, except for high-end product like chu-toro (fatty tuna) and awabi (abalone), includes two pieces of sushi (i.e., a ball of vinegared rice, or shari, with a raw or slightly cooked fish topping, called neta). Not surprisingly, these three — Kappa Sushi, Akindo Sushiro and Kura Sushi — dominate the market, but the real competition seems to be between the top two. Though Kappa is the biggest chain by far with 377 stores nationwide, Sushiro, with 295 outlets, made more money during the second half of 2010: ¥46.2 billion (20 percent more than the previous year) to Kappa's ¥45.5 billion (9 percent increase).
In a bid to win back the top spot, Kappa broke the magic ¥100 barrier by offering all plates except for desserts, soups and o-tsumami (side dishes, like edamame — boiled soy beans — and fried chicken) for ¥90 all-day Mondays through Fridays, and advertised the deal with a series of charmingly cheap-looking TV commercials. However, Sushiro's ascension was a more carefully calculated success and one that may be difficult to top by price-slashing. Sushiro's president, Kenichi Toyozaki, is a real sushi chef. He even does the demonstrations himself in the training videos for the chain's sushi chefs. Even more than a master of fish, he's a master of public relations. Whenever there's a fisheries-related issue in the news, such as the shortage of blue fin tuna, he welcomes questions from reporters who want to find out how such an issue will affect Japanese eating habits. Regardless of how self-serving his answers may be, the resulting publicity has lifted Sushiro's reputation above that of the competition. It is thought of as a company that values the "goodness of the materials" (sozai no yosa).
Sushiro's strategy may preclude the kind of price war that currently besets the beef bowl (gyudon) restaurant industry. Kappa's cost-cutting strategy was obviously carried out to regain the No. 1 position, but since it was only for a limited time, it may not be something the company can afford to do permanently. As it stands, both gyudon and kaiten sushi chains, which used to cater to salarymen almost exclusively, are now after the same customer base, families, which they've successfully wrested from family restaurants, the only eateries where, 10 years ago, you could take the kids and not attract withering stares from the other customers who wanted to eat in peace.
This entry was posted on Saturday, January 29th, 2011 at 1:25 pm	and is filed under Food & drink. You can follow any responses to this entry through the RSS 2.0 feed. Both comments and pings are currently closed.
I feel that the modern idea that certain (former) luxury items–o-toro maguro, the $49 cashmere sweaters at Costco–should be put within reach of the average consumer is a fallacy of our consumption-driven society. The popularity of those cashmere sweaters has contributed to severe desertification in Western China (the dust from which, ironically, reaches all the way to Costco's corporate home in Washington State), just as the still-growing popularity of inexpensive kaiten sushi (which can now be found well beyond Japan's borders) has contributed to rampant over-fishing of certain species that were once protected by their relative rarity on the market. Eventually, of course, the pendulum will swing back the other way, and we'll once again find the selection of 100 yen sushi limited to octopus, squid, tekka and tamago.
I like reading your stuff, Philip.
Of course kaiten-zushi chains can sell their sushi for such cheap prices because they often substitute. Alas, Japanese people can't taste the difference.
For example, the squid is often a deep sea variety that must be processed in factories in Thailand or China to remove the ammonia taste. The expensive fish with white flesh such as sea bream or flounder are in reality fresh water species such as large mouth bass or nile perch. The eggs for tamago sushi generally just processed yolks left over from cake factories.
Negi-toro is just kihada or binnaga tuna (the smallest species usually used for canned tuna) with salad oil added to make it seem fatty.
Most tuna they use is bought from special brokers who buy up tuna that was mishandled. A common technique to keep prices low is buying tuna with black meat because of improper freezing and exposure to air. A special chemical process is used to first bleach that black meat white and then a bright red dye is added.
There are several books and magazine articles in Japanese examining the dark side of why kaiten-zushi is so cheap.


text:
Home ♃ Recent Stories ☄ Wear Orange Weekend as Birmingham NAACP supports gun violence prevention
Wear Orange Weekend as Birmingham NAACP supports gun violence prevention
By Eric Taunton
The Alabama NAACP will hold three joint press conferences today in Birmingham, Huntsville, and Montgomery as part of National Gun Violence Awareness Day.
The Birmingham press conference will be held at 10 a.m. at the Birmingham Civil Rights Activists Committee Headquarters at 1707 4th Ave North.
This weekend (June 1-3) marks Wear Orange Weekend, a movement in support of gun violence prevention started in 2015.
"This is an effort to make a public statement from the NAACP that we are in favor of gun control legislation," said Hezekiah Jackson IV, president of the Birmingham metro National Association for the Advancement of Colored People. "At the end of the day we need to have a united front with students and young people everywhere who are crying out for gun control."
The Alabama chapters of the NAACP will join the Lee Daniels' Everytown Creative Council to help decrease the rates of mass-shootings and other forms of gun violence in the United States.
Everytown for Gun Safety has built a grassroots movement of more than three million people and won victories in nearly 40 states as part of its quest to help end gun violence in America.
Everytown is made up of actors, writers, designers, musicians, and artists and has established the Everytown Creative Council – joining forces to help amplify the movement to end gun violence.
"America will remain a deadly nation for our children, its schools caught in the crossfire, unless we insist politicians and the NRA curb their lobbyists efforts and allow the creation of policy that acts in the best interest of public safety," said Derrick Johnson, national president and CEO of the NAACP.
Benard Simelton, president of the Alabama state conference of the NAACP, said, "We would be remiss if we overlooked the senseless shootings of young black males by law enforcement. All gun violence needs to end. It is time to make sure that ALL of our children are safe."
The campaign started when 15-year-old Hayida Pendleton, a Chicago resident, was shot and killed two weeks after performing at President Obama's second inauguration. Pendleton's friends wore orange in honor of her death, thus turning orange into the official color of the gun control movement, Wear Orange being created soon after.
Studies conducted by the National Safety Council and the National Center for Health and Statistics conclude that American citizens have a 1-in-315 lifetime chance of death as a result of gun violence. That's more than 50 percent more likely than risk of death by car, truck, van and the combined risks of drowning, stabbing, choking on food, fire and smoke, airplane crashes, animal attacks, and natural disasters.
Previous articleHere Are Candidates on Tuesday's ballot In 2018 Jefferson County Races
Next articleBirmingham NAACP, supporters call for gun violence prevention

redpajama_set_name: RedPajamaCommonCrawl


text:
WNT11 is a direct target of early growth response protein 1
JuHwan Kim1, Euitaek Jung1, Sung Shin Ahn1, Hyunjin Yeo1, Jeong Yeon Lee1, Jeong Kon Seo2, Young Han Lee1,3 & Soon Young Shin1,3,*
1Department of Biological Sciences, Sanghuh College of Lifesciences, Konkuk University, Seoul 05029, 2Central Research Facilities, Ulsan National Institute of Science and Technology, Ulsan 44919, 3Cancer and Metabolism Institute, Konkuk University, Seoul 05029, Korea
Correspondence to: Tel: +82-2-2030-7946; Fax: +82-2-3437-9781; E-mail: shinsy@konkuk.ac.kr
Received: March 9, 2020; Revised: April 22, 2020; Accepted: July 6, 2020; Published online: December 31, 2020.
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences Abstract
WNT11 is a member of the non-canonical Wnt family and plays a crucial role in tumor progression. However, the regulatory mechanisms underlying WNT11 expression are unclear. Tumor necrosis factor-alpha (TNFα) is a major inflammatory cytokine produced in the tumor microenvironment and contributes to processes associated with tumor progression, such as tumor invasion and metastasis. By using site-directed mutagenesis and introducing a serial deletion in the 5'-regulatory region of WNT11, we observed that TNFα activates the early growth response 1 (EGR1)-binding sequence (EBS) in the proximal region of WNT11 and that the transcription factor EGR1 is necessary for the TNFα-induced transcription of WNT11. EGR1 bound directly to the EBSs within the proximal 5'-regulatory region of WNT11 and ectopic expression of EGR1 stimulated WNT11 promoter activity, whereas the knockdown of EGR1 expression by RNA interference reduced TNFα-induced WNT11 expression in T47D breast cancer cells. We also observed that mitogen-activated protein kinases (MAPK), extracellular signalregulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 kinase mediated TNFα-induced transcription of WNT11 via EGR1. Our results suggest that EGR1 directly targets WNT11 in response to TNFα stimulation in breast cancer cells.
Keywords: 5'-regulatory region, Early growth response 1, Mitogenactivated protein kinase, Tumor necrosis factor α, Wnt family member 11
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences INTRODUCTION
Wnt signaling pathways represent a group of highly evolutionarily conserved signal transduction pathways that control various physiological responses, including embryonic body axis formation, cell fate specification, cell proliferation, and cell migration (1). To date, at least 19 Wnt proteins have been identified in humans. All Wnt proteins bind and activate the Frizzled receptors, which transduce biological signals to the appropriate downstream targets (2). Wnt signaling is characterized by the β-catenin-dependent canonical pathway and the β-catenin-independent non-canonical pathway (3). Emerging evidence has implicated canonical and non-canonical Wnt signaling pathways in the development of human cancers, including gastrointestinal cancers, hepatocellular carcinoma, leuke-mia, melanoma, and breast cancers (4, 5).
WNT11 plays a crucial role in several morphological processes associated with embryogenesis, including anterior-posterior axis elongation through a β-catenin-dependent canonical or -independent non-canonical pathway (6). WNT11 promotes the proliferation and transformation of intestinal epithelial cells (7) and the downregulation of its receptor, frizzled-7, apart from reducing the survival, invasion, and metastasis of colon cancer cells (8). Consistent with the above, WNT11 overexpression promotes the proliferation and migration of several tumor cells, including cervical, breast, prostate, and colon cancer cells (9, 10). These results suggest that WNT11 signaling plays a crucial role in cell proliferation, invasion, and metastasis during carcinogenesis (11).
The transcription of WNT11 is controlled by multiple factors, including lymphoid enhancer-binding factor 1 (LEF1), GATA transcription factor (12), and E26 transforming sequence (ETS)-related gene (ERG), an ETS family transcription factor involved in hematopoiesis (13). In breast cancer cells, Chromatin immunoprecipitation-linked target site cloning was used to characterize WNT11 as an estrogen receptor target gene (14).
Tumor necrosis factor-alpha (TNFα) is a major inflammatory cytokine produced by tumor cells, tumor-associated fibroblasts, and infiltrated inflammatory cells in the tumor microenvironment (15). TNFα stimulates various inflammatory cytokines and chemokines and plays a crucial role in processes associated with tumor progression, including tumor invasion and metastasis (15-18). However, the role of TNFα in the regulation of WNT11 expression remains largely elusive. Owing to the role of TNFα in tumor progression, we hypothesized that TNFα may control WNT11 transcription. To evaluate this possibility, we isolated the 5'-regulatory region of the WNT11 gene and evaluated its expression levels in response to TNFα stimulation in the T47D breast cancer cell line. We observed that TNFα stimulates WNT11 transcription by activating the EGR1-binding cis-acting element within the 5'-regulatory region of WNT11. We also observed that the three major mitogen-activated protein kinases (MAPKs), extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 kinase, mediated TNFα-induced WNT11 expression via EGR1 upregulation.
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences RESULTS AND DISCUSSION
TNFα upregulates WNT11 expression in T47D breast cancer cells
We first examined the basal mRNA expression levels of WNT11 in various breast and colon cancer cells. MCF-7 and T47D breast cancer cells expressed relatively high levels of WNT11 mRNA, whereas no expression or low levels of expression were observed in non-transformed MCF10A breast epithelial cells and MDA-MB-231 breast cancer cells (Fig. 1A, left panels). Among the different colon cancer cells, the basal levels of WNT11 mRNA were relatively high in SW620 and HT29 cells, while the same was not observed in SW480 cells (Fig. 1A, right panels). We selected and characterized T47D cells exhibiting moderate WNT11 expression levels to investigate WNT11 mRNA expression following TNFα stimulation. An increase in the mRNA levels of WNT11 was detected within 12 h, followed by a reduction at 24 h after TNFα stimulation, whereas the mRNA levels of the glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) remained unaltered, as revealed by reverse transcription (RT)-PCR (Fig. 1B) and quantitative real-time PCR (qR-PCR) (Fig. 1C). Similar results were obtained in MDA-MB-231 breast cancer cells (Supplementary Fig. S1A) and HCT116 colon cancer cells (Supplementary Fig. S2B). TNFα-induced elevation of the WNT11 protein levels was confirmed by immunoblotting in T47D cells (Fig. 1D) and MDA-MB-231 cells (Supplementary Fig. S1B). Immunofluorescence studies also showed WNT11 antibody-staining after TNFα stimulation; however, no staining was observed in the PBS-treated control (Fig. 1E). These data suggest that WNT11 expression can be regulated by TNFα in T47D breast cancer cells.
Figure 1. Effect of TNFα on WNT11 expression. (A) Basal expression of WNT11 mRNA in various cancer cells. (B, C) T47D cells were treated with 10 ng/ml TNFα for 12 and 24 h. Total RNA was isolated, and the levels of WNT11 mRNA were determined using RT-PCR (B) and quantitative real-time PCR (qR-PCR) (C). The levels of GAPDH mRNA were determined as an internal control. In (C), the 0-time mRNA level was set to 1 after adjustment to the GAPDH levels. Data are presented as mean + S.D. (n = 3). *P = 0.0131; ***P < 0.001 by Dunnett's multiple comparisons test. (D) WNT11 levels were measured by immunoblotting. GAPDH levels were determined as an internal control. Band intensities were measured using the ImageJ software. After adjustment to the GAPDH levels, the 0-time band intensity was set to 1. Data are presented as mean + S.D. (n = 3). *P = 0.0127 (n = 3) by Dunnett's multiple comparisons test. (E) T47D cells were treated with PBS (control) or 10 ng/ml TNFα, and then incubated with antibodies against α/β-tubulin or WNT11 for 2 h, followed by incubation with Alexa Fluor 488-conjugated (for α/β-tubulin; green signal) and Alexa Fluor 555-conjugated (for WNT11; red signal) secondary antibodies for 30 min. Nuclear DNA was stained with 1 µg/ml Hoechst 33258 for 10 min (blue staining). White bar size, 50 µm. Arrows, WNT11-stained spots.
TNFα stimulates WNT11 promoter activity by activating the EGR1-binding cis-acting element in the proximal promoter region of the 5'-regulatory region
To investigate whether TNFα stimulates WNT11 transcription, a fragment of the 5'-regulatory region spanning nucleotides −997 to +74 was isolated, and the effect exerted by TNFα on the activation of the WNT11 promoter was assessed. We ob-served that TNFα significantly enhanced the promoter-reporter activity (Fig. 2A), suggesting that TNFα enhances WNT11 expression at the transcriptional level. To delineate the promoter region that is responsible for TNFα-induced WNT11 promoter activation, we designed a series of deletion constructs and mapped the TNFα response region. Upon TNFα stimulation, the shortest reporter construct (−53/+74) continued to exhibit induction (Fig. 2A), suggesting that the TNFα-inducible region of the WNT11 promoter is located between nucleotides −53 and +74.
Figure 2. Role of EGR1 in TNFα-induced WNT11 expression. (A) HEK293 cells were transiently transfected with 0.2 µg of a series of 5'-deletion constructs. After treating with vehicle (PBS) or 10 ng/ml TNFα, luciferase activities were measured. (B) Sequence elements of EBS between −22 to +18 in the −53/+73 construct (top sub-figure). HEK293 cells were transiently transfected with site-specific mutants derived from the −53/+74 construct. After treatment with the vehicle (PBS) or with 10 ng/ml TNFα, luciferase activities were measured. EBS, EGR1-binding sequence; mtEBS, mutation of EBS. Asterisk (*) indicates a mutation site. Luciferase activity data are presented as mean + S.D. (n = 3). ***P < 0.001 by Sidak's multiple comparisons test. (C) T47D cells were treated with 10 ng/ml TNFα for 0, 3, and 6 h. After preparing the nucleus-enriched fractions, EGR1 levels were measured by immunoblotting. (D) HEK293 cells were transiently cotransfected with 0.2 µg of the −150/+74 construct and EGR1 expression plasmid (pcDNA3.1/Egr1). Forty-eight hours later, the cells were harvested, and luciferase activities were measured. Data are presented as mean + S.D. (n = 3). The transfected EGR1 levels were confirmed by immunoblotting. (E and F) T47D transfectants stably expressing control scrambled shRNA (shCT) or EGR1 shRNA (shEgr1) were treated with 10 ng/ml TNFα for 0.5 or 12 h. Expression levels of the EGR1 and WNT11 mRNAs were measured by RT-PCR (E), and WNT11 mRNA levels were quantitated by quantitative real-time PCR (qR-PCR) (F). qR-PCR data are presented as mean + S.D. (n = 3). NS, not significant; ***P < 0.001 by Sidak's multiple comparisons test.
To identify the functional cis-acting element responsible for TNFα-induced WNT11 gene transcription, we analyzed the transcription factor regulatory sequences between nucleotides −53 and +74 using a web-based MatInspector transcription factor search tool (http://www.genomatix.de/). We found two putative early growth response protein 1 (EGR1)-binding sequences (EBSs) spanning nucleotides −22 to +18 (named EBS-1, −22 to −4, and EBS-2, −1 to +18) (Fig. 2B). EGR1, also known as zinc finger protein 225 (Zif268) or nerve growth factor-induced clone A (NGFI-A), is a Cys2His2-type zinc finger protein that exhibits Fos-like induction kinetics in response to various mitogenic stimuli and DNA damage signals (19). The target genes of EGR1 are associated with multiple physiological responses, including cell proliferation, differentiation, apoptosis, and inflammation, in a variety of cell types (19, 20). However, the role of EGR1 in WNT11 transcription remains elusive.
To assess the functional role of these putative EBSs in TNFα-induced WNT11 transcription, we introduced site-directed mutations into the EBSs. Damage to the EBS-1 (mtEBS-1) or EBS-2 (mtEBS-2) motifs in the pWnt11-Luc(−53/+74) significantly reduced TNFα-induced reporter activity compared to that in the wild-type (WT) construct (Fig. 2B, bottom graph). Damage to both EBS-1 and EBS-2 sites resulted in an almost complete loss of TNFα inducibility.
TNFα induces EGR1 expression in various cell types (21-24). Consistently, we observed that EGR1 expression increased in a time-dependent manner upon TNF-α stimulation (Fig. 2C). To determine whether EGR1 transactivates WNT11, we transfected the −150/+74 construct into T47D cells, along with an expression plasmid for EGR1. Exogenous overexpression of EGR1 enhanced the WNT11 promoter-reporter activity in a plasmid concentration-dependent manner (Fig. 2D). These results suggest that EGR1 can transactivate WNT11 in T47D cells.
To further investigate the role of EGR1 in TNFα-induced WNT11 transcription, we established T47D transfectants expressing lentiviral shRNA against EGR1 (T47D/shEgr1) and a scrambled control (T47D/shCT). The stable knockdown of basal and TNFα-induced expression of EGR1 mRNA was confirmed by RT-PCR after TNFα treatment (Fig. 2E, top panels). The ability of TNFα to induce the mRNA expression of WNT11 was substantially attenuated when EGR1 expression in T47D/shEgr1 was reduced compared to that in T47D/shCT cells (Fig. 2E, bottom panels). qR-PCR analysis revealed that TNFα elevated WNT11 mRNA expression by 3.17 ± 0.306-fold in T47D/shCT cells, while it reduced WNT11 mRNA expression by 0.600 ± 0.100-fold in T47D/shEgr1 cells (Fig. 2F). These results suggest that the EBS motifs in the promoter region are necessary for the TNFα-induced transcription of WNT11 .
EGR1 binds to the EBS motif and transactivates WNT11 transcription
To determine whether the EBS motif can serve as a binding site for EGR1, we performed the non-radioactive electrophoretic mobility shift assay (EMSA) using Sf21 insect cell lysates overexpressing EGR1 (Sf21/Egr1) and biotinylated EBS oligo-nucleotide probes. Biotinylated consensus EBS oligonucleotides were used as a positive control. We observed that the biotinylated EBS-1 (Fig. 3A) and EBS-2 (Fig. 3B) probes formed DNA-protein complexes. These complexes competed with a molar excess of an unlabeled probe. These data suggest that EGR1 binds specifically to the EBS motifs within the proximal WNT11 promoter region.
Figure 3. Binding of EGR1 to putative EBS motifs of WNT11. (A, B) EBS-1 (A) and EBS-2 (B) probes were incubated with lysates of Sf21 cells expressing EGR1 (Sf21/EGR1) or with the wild-type (Sf21/WT) in the presence or absence of an unlabeled probe (cold competitor). Samples were separated by non-denaturing 6% acrylamide gel electrophoresis and visualized using streptavidin-conjugated horse-radish peroxidase. (C and D) T47D cells were treated with 10 ng/ml TNFα for 1 h or were left untreated. Nuclear extracts were prepared and incubated with biotinylated EBS-1 (C) or EBS-2 (D), followed by the addition of streptavidin-conjugated agarose beads. After 1 h, the agarose beads were washed and boiled for 5 min with 2 × Laemmli sample buffer. Immunoblotting was performed using anti-EGR1 or anti-PCNA antibodies. Input, an aliquot of the nuclear extract; DAPA, DNA-affinity precipitation assay.
To further corroborate the binding of EGR1 to the EBS motifs in WNT11, we adopted an alternative approach using the DNA-affinity precipitation assay (DAPA). Biotinylated EBS-1 and EBS-2 oligonucleotides were incubated with nuclear extracts from T47D cells. Upon TNFα stimulation, the levels of EGR1 and nuclear protein PCNA increased in the whole nuclear extract (input) (Fig. 3C and D; bottom panels). After pull-down with streptavidin-agarose beads, oligonucleotide-binding proteins were eluted and immunoblotted with an anti-EGR1 or anti-PCNA antibody. We observed that while EBS-1 and EBS-2 bound EGR1 in response to TNFα stimulation, they did not bind PCNA (Fig. 3C and D; top panels). These data suggest that the proximal EBS sites located between nucleotides −22 and +18 in the WNT11 promoter region bind EGR1; however, they do not exhibit any off-target effect.
MAPKs mediate TNFα-induced WNT11 expression via EGR1 upregulation
Transcription factor ETS like-1 protein (ELK1) and serum response factor (SRF) complex transactivate the serum response element (SRE) in the 5'-regulatory region of the EGR1 gene. MAPK pathways control EGR1 expression via phosphorylation of ELK-1 in response to TNFα stimulation in several cell types (21, 25, 26). To determine which MAPK pathways are responsible for EGR1-mediated WNT11 expression, we validated the active status of TNFα-induced MAPKs in serum-starved T47D cells. The phosphorylation levels of ERK1/2 at Thr-202/Tyr-204, JNK1/2 at Thr-183/Tyr-185, and p38 kinase at Thr-180/Tyr-182 increased within 30 min in response to TNFα stimulation (Fig. 4A). Pretreatment with MAPK inhibitors, including the MAPK kinase (MEK) inhibitor U0126, JNK inhibitor SP600125, and p38 kinase inhibitor SB203580, substantially abrogated the TNFα-induced accumulation of EGR1 proteins (Fig. 4B). Consistent with EGR1 expression results, all MAPK inhibitors significantly reduced TNFα-induced WNT11 expression; in particular, U0126 and SP600125 showed a strong inhibitory effect compared to SB203580 (Fig. 4C). These data suggest that all three major MAPK pathways contribute to EGR1-mediated WNT11 expression in response to TNFα exposure in T47D breast cancer cells.
Figure 4. Role of MAPKs in TNFα-induced WNT11 expression. (A) T47D cells were treated with 10 ng/ml TNFα for various durations (0-1200 min). Immunoblotting was performed using a phospho-specific antibody against ERK1/2 (Thr202/Tyr204), JNK1/2 (Thr183/Tyr185), or p38 kinase (Thr180/Tyr182). GAPDH levels were deter-mined as an internal control. (B, C) T47D cells were pretreated with U0126 (10 µM), SB203580 (20 µM), or SP600125 (20 µM) for 30 min and then stimulated with 10 ng/ml TNFα. After 1 and 12 h, the levels of EGR1 (B) and WNT11 mRNAs (C) were measured by immunoblotting and RT-PCR, respectively. The band intensities were measured relative to the GAPDH levels using the ImageJ software. Bars represent the mean ± S.D. (n = 3). *P = 0.0149; **P = 0.0017; ***P < 0.001 by Dunnett's multiple compa-rison test.
The current study identified the role of EGR1 in TNFα-induced WNT11 expression in T47D human breast cancer cells. In response to TNFα stimulation, EGR1 directly bound to the EBS in the WNT11 promoter region and stimulated WNT11 transcription. All three major MAPKs, ERK, JNK, and p38 kinases, were involved in TNFα-induced EGR1 upregulation. We conclude that WNT11 is a direct target of the EGR1 in response to TNFα stimulation, further expending our understanding of the regulatory mechanism of WNT11 expression in the tumor microenvironment. As WNT11 activates cancer cell motility and metastasis (27) and reduction of WNT11 expression decreases the ability of breast cancer cells to migrate (9), EGR1-regulated WNT11 expression can be a promising therapeutic target for suppressing metastasis of breast cancer. Further in vivo studies are warranted to verify the clinical relevance of targeting the TNFα-EGR1-WNT11 axis to prevent tumor metastasis in breast cancer.
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences MATERIALS AND METHODS
Cell and reagents
Detailed cells and reagents used in this study were described in Supplementary materials.
Reverse transcription-PCR (RT-PCR)
Total RNA was isolated using a TRIzol RNA extraction kit (Invitrogen). First-strand cDNA was synthesized using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). The PCR conditions were as follows: hold for 5 min at 94°C, followed by 30 cycles of denaturation at 94°C (30 s), annealing at 55°C (30 s), and elongation at 72°C (1 min). The gene-specific PCR primers were as follows: WNT11 forward, 5' GAT CCC AAG CCA ATA AAC TGA TGC GTC T-3'; WNT11 reverse, 5'-GTC TTG TTG CAC TGC CTG TCT TGT GTC C-3'; GAPDH forward, 5' CCAAGGAGTAAGAAACCCTGGAC-3'; GAPDH reverse, 5'-GGGCCGAGT TGGGATAGG G-3'. The amplified products were electrophoresed in 1% agarose gel.
Immunoblot analysis was performed as described previously (21). Detailed experimental methods were described in Supplementary materials.
T47D cells cultured on coverslips were either treated with the vehicle (PBS) or 10 ng/ml TNFα for 18 h, followed by fixation, permeabilization, and incubation with primary antibodies against WNT11 and α/β-tubulin (for counterstaining). After 2 h, the cells were incubated for 30 min with secondary antibodies conjugated with Alexa Fluor 555 (for α/β-tubulin; red signal) and Alexa Fluor 488 (for WNT11, green signal). Nuclear DNA was stained using 1 µg/ml Hoechst 33258 for an additional 10 min (blue signal). Fluorescently-stained cells were examined under an EVOS FL fluorescence microscope (Advanced Microscopy Group, Bothell, WA, USA).
Construction and mutagenesis of human WNT11 promoter-reporter constructs
Detailed experimental methods for the generation of the human WNT11 promoter-reporter constructs and primer sequences were described in Supplementary materials.
WNT11 promoter-reporter assay
HEK293T cells cultured in 12-well plates were transfected with 0.2 µg WNT11 promoter constructs using Lipofectamine 2000 (Invitrogen), according to the manufacturer's instructions. Detailed experimental methods for promoter-reporter assay were described in Supplementary materials.
Electrophoretic mobility shift assay (EMSA)
EMSA was performed using a LightShift Chemiluminescence EMSA kit, according to the manufacturer's instructions (Thermo-Fisher Scientific, Waltham, MA, USA). Biotin-labeled deoxyoligonucleotide probes specific for EBS-1 (5'-CCT TAA CCC GCC GCC TCC GCT CTC C-biotin-3'), EBS-2 (5'-AGG CGG CGC GCG GGC GGG GGA GGC G-biotin-3'), and the consensus EBS sequence (5'-GGA TCC AGC GGG GGC GAG CGG GGG CGA-biotin-3') were obtained from Macrogen. For the competition assay, 1 and 2.5 pmole of the unlabeled EBS-1 and EBS-2 probes (competitor) were added, respectively. DNA-protein complexes were electrophoresed in non-denaturing 6% polyacrylamide gels and visualized using an ECL chemiluminescence system (GE Healthcare Life Science).
DNA-affinity precipitation assay (DAPA)
DAPA was performed as reported previously (28). Briefly, nuclear extracts (35 µg) were incubated with 500 pmol of biotinylated EBS-1 (5ʹ-biotin-CCT TAA CCC GCC GCC TCC GCT CTC C-3') or EBS-2 (5ʹ-biotin-AGG CGG CGC GCG GGC GGG GGA GGC G-3') oligonucleotide and streptavidin-conjugated agarose beads (Invitrogen) for 1 h, as described previously (29). After washing thrice with PBS, the pellets were boiled with 2 × Laemmli sample buffer and immunoblot analysis was performed using antibodies against EGR1 or PCNA (off-target control).
EGR1 silencing using RNA interference
T47D cells were incubated with lentiviral shRNA (TRCN_0000273850; MISSIONⓇ shRNA; Sigma-Aldrich) targeting EGR1, according to the manufacturer's instructions. After 2 wk, the silencing of EGR1 expression was determined by immuno-blotting.
Statistical significance of results was determined using one-way analysis of variance (ANOVA), followed by Sidak's multiple comparisons test or Dunnett's multiple comparisons test using the GraphPad Prism version 8.3.1 software (Graph-Pad Software Inc., La Jolla, CA, USA). A P-value < 0.05 was considered statistically significant.
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences Supplemental Materials
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences ACKNOWLEDGEMENTS
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning (Grant no. 2020R1A2C1005845), Republic of Korea. This paper was supported by the KU Research Professor Program of Konkuk University and by the Konkuk University Researcher Fund in 2019.
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences CONFLICTS OF INTEREST
Other SectionsAbstractINTRODUCTIONRESULTS AND DISCUSSIONMATERIALS AND METHODSSupplemental MaterialsACKNOWLEDGEMENTSCONFLICTS OF INTERESTReferences References
Goldsberry WN, Londono A, Randall TD, Norian LA and Arend RC (2019) A review of the role of wnt in cancer immunomodulation. Cancers (Basel) 11, 771
Nusse R and Clevers H (2017) Wnt/beta-catenin signaling, disease, and emerging therapeutic modalities. Cell 169, 985-999
van Andel H, Kocemba KA, Spaargaren M and Pals ST (2019) Aberrant Wnt signaling in multiple myeloma: molecular mechanisms and targeting options. Leukemia 33, 1063-1075
Zhan T, Rindtorff N and Boutros M (2017) Wnt signaling in cancer. Oncogene 36, 1461-1473
Shi J, Zheng H and Yuan L (2019) High NDRG3 expression facilitates HCC metastasis by promoting nuclear translocation of beta-catenin. BMB Rep 52, 451-456
Tao Q, Yokota C, Puck H et al (2005) Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos. Cell 120, 857-871
Ouko L, Ziegler TR, Gu LH, Eisenberg LM and Yang VW (2004) Wnt11 signaling promotes proliferation, transformation, and migration of IEC6 intestinal epithelial cells. J Biol Chem 279, 26707-26715
Ueno K, Hazama S, Mitomori S et al (2009) Down-regulation of frizzled-7 expression decreases survival, invasion and metastatic capabilities of colon cancer cells. Br J Cancer 101, 1374-1381
Dwyer MA, Joseph JD, Wade HE et al (2010) WNT11 expression is induced by estrogen-related receptor alpha and beta-catenin and acts in an autocrine manner to increase cancer cell migration. Cancer Res 70, 9298-9308
Wei H, Wang N, Zhang Y, Wang S, Pang X and Zhang S (2016) Wnt-11 overexpression promoting the invasion of cervical cancer cells. Tumour Biol 37, 11789-11798
Uysal-Onganer P and Kypta RM (2012) Wnt11 in 2011 - the regulation and function of a non-canonical Wnt. Acta Physiol (Oxf) (Oxf), 52-64
Katoh M and Katoh M (2009) Integrative genomic analyses of WNT11: transcriptional mechanisms based on canonical WNT signals and GATA transcription factors signaling. Int J Mol Med 24, 247-251
Mochmann LH, Bock J, Ortiz-Tanchez J et al (2011) Genome-wide screen reveals WNT11, a non-canonical WNT gene, as a direct target of ETS transcription factor ERG. Oncogene 30, 2044-2056
Lin Z, Reierstad S, Huang CC and Bulun SE (2007) Novel estrogen receptor-alpha binding sites and estradiol target genes identified by chromatin immunoprecipitation cloning in breast cancer. Cancer Res 67, 5017-5024
Ham B, Fernandez MC, D'Costa Z and Brodt P (2016) The diverse roles of the TNF axis in cancer progression and metastasis. Trends Cancer Res 11, 1-27
Balkwill F (2009) Tumour necrosis factor and cancer. Nat Rev Cancer 9, 361-371
Coussens LM and Werb Z (2002) Inflammation and cancer. Nature 420, 860-867
Joyce JA and Pollard JW (2009) Microenvironmental regulation of metastasis. Nat Rev Cancer 9, 239-252
Gashler A and Sukhatme VP (1995) Early growth response protein 1 (Egr-1): prototype of a zinc-finger family of transcription factors. Prog Nucleic Acid Res Mol Biol 50, 191-224
Milbrandt J (1987) A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor. Science 238, 797-799
Son SW, Min BW, Lim Y, Lee YH and Shin SY (2008) Regulatory mechanism of TNFalpha autoregulation in HaCaT cells: the role of the transcription factor EGR-1. Biochem Biophys Res Commun 374, 777-782
Cao XM, Guy GR, Sukhatme VP and Tan YH (1992) Regulation of the Egr-1 gene by tumor necrosis factor and interferons in primary human fibroblasts. J Biol Chem 267, 1345-1349
Grimbacher B, Aicher WK, Peter HH and Eibel H (1998) TNF-alpha induces the transcription factor Egr-1, pro-inflammatory cytokines and cell proliferation in human skin fibroblasts and synovial lining cells. Rheumatol Int 17, 185-192
Chaudhary LR, Cheng SL and Avioli LV (1996) Induction of early growth response-1 gene by interleukin-1 beta and tumor necrosis factor-alpha in normal human bone marrow stromal an osteoblastic cells: regulation by a protein kinase C inhibitor. Mol Cell Biochem 156, 69-77
Utreras E, Futatsugi A, Rudrabhatla P et al (2009) Tumor necrosis factor-alpha regulates cyclin-dependent kinase 5 activity during pain signaling through transcriptional activation of p35. J Biol Chem 284, 2275-2284
Mishra JP, Mishra S, Gee K and Kumar A (2005) Differential involvement of calmodulin-dependent protein kinase II-activated AP-1 and c-Jun N-terminal kinase-activated EGR-1 signaling pathways in tumor necrosis factor-alpha and lipopolysaccharide-induced CD44 expression in human monocytic cells. J Biol Chem 280, 26825-26837
Luga V, Zhang L, Viloria-Petit AM et al (2012) Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration. Cell 151, 1542-1556
Wu KK (2006) Analysis of protein-DNA binding by streptavidin-agarose pulldown. Methods Mol Biol 338, 281-290
Kim J, Jung E, Choi J, Min DY, Lee YH and Shin SY (2019) Leptin is a direct transcriptional target of EGR1 in human breast cancer cells. Mol Biol Rep 46, 317-324
2020R1A2C1005845


text:
Karol Seifert ps. Andrzej Korycki (ur. 31 marca 1957) – polski nauczyciel i działacz opozycji w PRL.

Życiorys
Absolwent VI Liceum Ogólnokształcącego im. Ignacego Jana Paderewskiego w Poznaniu. W 1980 roku ukończył historię na Uniwersytecie im. Adama Mickiewicza w Poznaniu. W listopadzie 1980 roku został członkiem NSZZ Solidarność. W lipcu 1981 był delegatem na I Walne Zebranie Delegatów NSZZ Solidarności Regionu Wielkopolska. W latach 1982 - 1987 współorganizował spotkania dla nauczycieli związanych z Solidarnością w Towarzystwie Chrystusowym.

Redagował prasę podziemną: Nauczyciel, Strzecha, Niepodległość. Był przedstawicielem Solidarności w społecznej komisji ds. niszczenia akt Komitetu Wojewódzkiego PZPR w Poznaniu; a w czerwcu 1991 roku podczas rewizji jego mieszkania zabezpieczono uratowane akta PZPR.

W latach 1999 - 2002 pełnił funkcję wielkopolskiego kuratora oświaty. Od 2002 roku jest dyrektorem VI Liceum Ogólnokształcącego im. Ignacego Jana Paderewskiego w Poznaniu, pracował również w Zespole Szkół Budowlanych nr 1 w Poznaniu, gdzie w latach 80. założył koło "Solidarności".

Po 1989 roku politycznie związany był z RS AWS (1997–2001), a następnie z PO (2001–2008).

W 2019 roku został odznaczony Krzyżem Kawalerskim Orderu Odrodzenia Polski.

Przypisy

Absolwenci VI Liceum Ogólnokształcącego im. Ignacego Jana Paderewskiego w Poznaniu
Absolwenci Wydziału Historycznego Uniwersytetu im. Adama Mickiewicza w Poznaniu
Kuratorzy oświaty III Rzeczypospolitej
Odznaczeni Krzyżem Kawalerskim Orderu Odrodzenia Polski (III Rzeczpospolita)
Politycy Akcji Wyborczej Solidarność
Politycy Platformy Obywatelskiej
Polscy nauczyciele historii
Urodzeni w 1957
Wydawcy podziemni w PRL (1976–1989)

redpajama_set_name: RedPajamaWikipedia


text:
Bernanke wants Fed to keep duties
FCC approves Frontier Communications' bankruptcy plan
Wilton apartment complex sold for $35 million
Cocktail startup sets up HQ in CT city where SpikedSeltzer concocted
Norwalk startup's invention to be featured on 'The View'
Former Fortune 500 manufacturer moves HQ to Norwalk
After leading Frontier into bankruptcy, CEO steps aside
Norwalk movie theater to reopen Friday
A gingerbread house boulevard in Norwalk — 50 years under development
Washington Street developer says viability of mall, South Norwalk go...
Artists craft new holiday tradition in South Norwalk
By JEANNINE AVERSA
and JIM KUHNHENN
Federal Reserve Chairman Ben Bernanke urged Congress Wednesday to let the Fed keep all of its banking oversight, arguing that information gleaned from that process helps the central bank guide the economy.
Testifying at a House hearing, Bernanke waged a fresh battle against Senate efforts to scale back the Fed's role in overseeing the nation's banks.
The Fed boss argued that policymakers factor information they get from the Fed's role as bank regulator into their decisions on interest rates. And, Bernanke said its banking duties give the Fed insights into the health of the entire banking system.
"The insights provided by our role in supervising a range of banks, including community banks, significantly increases our effectiveness in making monetary policy and fostering financial stability," Bernanke told the House Financial Services Committee.
Bernanke's testimony comes as the Fed faces a significant shift in its supervisory duties.
In an effort to overhaul the nation's financial regulatory structure, Senate Banking Committee Chairman Christopher Dodd, D-Conn., has offered legislation that would strip the Fed of its power to supervise state-chartered banks and bank holding companies with assets of less than $50 billion.
That would leave the Fed with 35 of the biggest bank holding companies under its supervision.
Critics blame lax regulation at the Fed and at other agencies for contributing to the financial and economic crisis. Bernanke once again acknowledged deficiencies, and said the Fed is taking steps to beef up oversight.
It currently oversees about 5,000 bank holding companies, about 850 smaller banks that are both state-chartered and are members of the Federal Reserve system and some foreign banks operating in the United States.
Dodd's bill, however, would also give the Fed new powers to oversee nonbank financial firms that are so large and interconnected that their failure could pose a risk to the economy.
Such firms could include insurance giant American International Group Inc., or General Electric Co.'s GE Capital business.
Bernanke said the Fed is "quite concerned" about losing oversight of small banks and essentially becoming the "too big to fail regulator" under the Dodd bill. "We want connections to Main Street as well as Wall Street," said Bernanke.
With its narrower authority, the Fed's system of 12 regional banks could face profound changes. The Kansas City Federal Reserve Bank and the St. Louis Federal Reserve Bank, for instance, would have no banks under their supervision.
The Obama administration has supported a broader supervisory role for the Fed. Legislation passed by the House overhauling the regulatory landscape doesn't trim the Fed's banking duties.
Former Fed Chairman Paul Volcker, who currently serves as economic adviser to Obama, also argued for the Fed to retain supervision over all the banks it now oversees and cited similar reasons as Bernanke.
"It would be a really grievous mistake," Volcker said of curtailing some of the Fed's banking oversight.
"Something important, if less obvious would also be lost if the present limited responsibilities for smaller member banks were to be ended. The Fed's regional roots would be weaker and a useful source of information lost," Volcker said.
Small banks also made the case to continue to be regulated by the Fed.
Jeffrey Gerhart, president of Bank of Newman Grove, said the Fed would "lose valuable information about local economies," which informs interest rate decisions, if the Fed no longer supervised small banks.
Dodd's bill would also place an independent consumer watchdog inside the Fed. The Consumer Financial Protection Bureau, however, would have its own director appointed by the president and would not fall under the authority of Bernanke.
The administration has called for a freestanding consumer agency, an approach that would strip the Fed of its consumer-protection responsibilities. The House version of a financial revamp resembles the administration's approach. Bernanke has argued that despite past weaknesses, the Fed should retain its consumer-protection duties. He didn't address the matter in his testimony prepared for Wednesday.
Anil Kashyap, professor of economics and finance at the University of Chicago Booth School of Business, said that consumer-protection oversight should be handled by some other agency, not the Fed.
But he backed Bernanke's pitch for the Fed to keep its banking oversight. "Stripping the Fed of its role in bank supervision would be a step in the wrong direction," Kashyap said.
Allan Meltzer, a professor at Carnegie-Mellon University, doubted any regulator can prevent all risks to the financial system. "Setting up an agency to prevent systemic risk without a precise, operational definition is just another way to pick the public's purse," he said.
He also worried that the Fed's extraordinary money pumped out during the financial crisis will eventually spur inflation. "I believe we are headed for higher inflation, not immediately, but later," he warned.


Talking with healthcare providers about cancer can be overwhelming. It can be hard to take in all of the information. It helps to be prepared. Make a list of questions and bring them to your appointments. Write the answers down in a notebook. Ask how the treatment will change your daily life, including your diet, and how you will look and feel after treatment. Ask how successful the treatment is expected to be, and what the risks and possible side effects are. You may also want to ask a friend or family member to go with you. He or she can take notes and write down the answers, and also ask questions you may not think of. You can also ask your healthcare provider if you can record the conversation.
What kind of ovarian cancer do I have?
What are the treatment choices?
What is the success rate of this treatment for my type and stage of ovarian cancer?
Are there any clinical trials that I can apply for?
Will my health insurance cover treatment?
How much can I expect to have to pay for treatment?
Does someone need to go with me during treatments?
If I need surgery, how long will I need to stay in the hospital?
Will treatment affect my ability to become pregnant?
Are there support groups nearby that I can join?
Should other women in my family be tested for ovarian cancer?
Should I get genetic testing for my cancer?
How can I get a copy of the treatment summary and a follow-up care plan (survivorship care plan)?


FOX 5 NY News streams daily. With comment live blogs, video from the Nottinghamshire Live team, pictures formerly the Nottingham Setup File Download Game PC Full Version For Free Offline. The Simpsons: Hit & Run was released on Sep 16,. Tip off your NBA season with a purchase of Clippers tickets and watch one of the most exciting teams in the NBA fight for a world championship.
How To Install The Simpsons Hit and Run Without Problem on windows platforms. Latest news sport events from Nottingham.
Votre portail d' information sur l' actualité les voyages, la culture, le showbiz, la finance, la santé, les sports, les technologies la mode et l' habitation. The Simpsons Hit and Run Free Download PC game setup in single direct link for Windows. There are 527 Arcade games on such as Uncle Express Go Go, Helix Jump 3 Neon Dunk. Free download the simpsons hit and run pc.
DaVincis Skycycle! Follow video tutorial guide steps to install The Simpsons Hit Run Subnautica for FREE on PC – Released on December 16, Subnautica is an open world survival exploration game where you descend into the depths of an alien underwater world filled with wonder peril. Ookla Accessibility Statement.
The Deer Hunter Game for PC comes with enhanced hunting experience – the animals make movements like jumping when nning time: 21– 24 minutes: Production company( s) : Gracie Films ( 1989– present) 20th Century Fox Television Klasky Csupo ( 1989– 1992) Film Roman ( 1992– ) Fox Television Animation ( – present) The Curiosity Company ( – present, uncredited) AKOM. OceanOfGames Ocean Of Games OceanGames PC Need for Speed Most Wanted PC Game Overview Need for Speed Most Wanted is a racing game that has been developed [. You can search all genre of games such as action horror, simulation, adventure, fighting, sports, strategy, racing, shooting role playing game for laptop for free.
125% Service- Providers Marketing Software for Win95:. Billy has been working on a new invention in this episode. Blur PC Game is an amazing arcade racing video game with scintillating visuals.
Wrecking Ball Physics! Free download the simpsons hit and run pc. It is based on the animated tv series The Simpsons was released for the PlayStation 2, in Europe on October 31, Microsoft Windows in North America on September 16, Xbox, GameCube , in Japan on December 25 . This is big collection of full version games for computer or pc notebook. It is a latest game from Most wanted series. It' s an interesting action and adventure PC game. Avi) quality free, without registration Truck driving games bus driving jeep driving games for. Need for Speed Most Wanted Free Download PC Game setup in single direct link for windows.
Jan 17 releasing on January 18 for PS4 , Xbox One, · Get a look at the heavily armed , armored Su- 34 Fullback from the upcoming game on February 1 for PC. Check the Live page to watch fullscreen and view the 0- 9. Download games free for windows XP Vista 8.
It was released on September 16,. Fun driving games online: Free driving games for kids car driving games, girls to play no download.
At Ookla, we are committed to ensuring that individuals with disabilities can access all of the content at Angeles Clippers Ticket Information. Free download the simpsons hit and run pc. His latest invention is the Photachyon Tranceiver, a radio that can reviece signals from anywhere in the er Hunter For PC Free Download Full Version Overview.
0: User name: dumbo Registration Key:. The most popular completely free games to download iOS phone , play on your Android tablet! The Simpsons: Hit & Run Free Download PC Game pre- installed in direct link. Fans all over LA are looking for seats at the Staples Center with the franchise on the rise our assortment of LA Clippers tickets extends from the first game of the year to the postseason.
Blur Free Download PC Game setup in single direct link for Windows. TV Show Shadowhunters ( season 1 3) download full episodes , 720p, watch in HD ( 480p 1080p. The Simpsons Hit and Run Overview. The Simpsons: Hit & Run is a action- adventur game developed by Radical Entertainment and published by Vivendi Universal Games.
1- 4- All HTML editor v1. The Simpsons: Hit & Run is a Grand Theft Auto clone action- adventure video game developed by Radical Entertainment for GameCube, published by Vivendi Universal Games, Xbox , Microsoft is based on the American animated sitcom The Simpsons, PlayStation 2 is the twenty- second installment in the Simpsons series.
Flash Games play arcade flash games, action flash games, sports flash games, puzzle games flash games. Oct 22 · Sonic Adventure 2 PC download full version review cheats battle for android windows 8 7 XP System Requirements free pc game download. Learn how to download install Subnautica for free in this article be sure to share this site with your friends. Diamond Hollow 2!
We have chosen the best Arcade games which you can play online for free.
The Simpsons Hit and Run Overview. Scrap Mechanic Free Download for PC – Released on January 19,, Scrap Mechanic is a creative sandbox game with action and adventure.
This game will take you into the world where you will create your own adventure.


text:
Home News Water
India gives green light to build country's largest dam
Largest dam in Indian history will be built on a tributary of the Brahmaputra river but, with no public consultation or study of downstream impacts, reports Chandan Kumar Duarah.
Hydropower projects are queueing to set up dams along the Brahmaputra river, which comes from the Tibetan Plateau through China, India and Bangladesh. In India alone, around 168 massive dams are expected to produce 57,000 megawatts of hydropower in Assam and Arunachal Pradesh states. Image: Shutterstock
thethirdpole.net
India's Prime Minister Narendra Modi has given the green light to construction of the 3,000 megawatt Dibang hydropower project in the ecologically fragile region of Arunachal Pradesh, north-east India.
The proposed dam on the Dibang River, a major tributary of the Brahmaputra close to the border with China, will be India's largest and one of the world's tallest dams.
The Forest Advisory Committee of India's Ministry of Environment and Forests cleared the dam last week, subject to a reduction in the dam height by 20 metres from the originally envisaged 288 metres.
The committee has refused environmental clearances for the project twice before on the grounds it would destroy forests and impact the Dibru-Saikhowa National Park downstream in Assam state.
The National Hydroelectric Power Corporation (NHPC) –the company behind the dam – submitted several revised proposals to the ministry, reducing the amount of forest cleared and the height of the dam structure. But on August 28 the Forest Advisory Committee rejected the latest proposal to submerge more than 45 square kilometres of forest land for the project.
Experts also say no proper overall plan has been put in place: though India and China have signed a limited agreement to data on river flow, there is no specific deal on managing the Brahmaputra's waters
The committee concluded the "ecological and social costs of destroying a vast tract of forest land which is a major source of livelihood for the state's tribal population would far outweigh the benefit likely to accrue from the project."
However, days later the Prime Minister's principal secretary sent a letter to the environment secretary on September 3 to "clear the project expeditiously" as per the decision of the Cabinet Committee on investment.
In its pre-election campaign Modi's Bharatiya Janata Party promised not to go ahead with mega dams and people supported the party enthusiastically.
The Krishak Mukti Sangram Samittee (KMSS) – a farmers' organisation spearheading the anti-dam movement in Assam – is now accusing the prime minister of being a traitor.
Others organisations agitating against mega dams are also threatening to protest against the 16,000 crores (US$2.6 million) Dibang project. Most of the power produced will be exported to help ease power shortages elsewhere in India.
Opening the floodgates
The central government has decided to build the project without any public consultation or study of the potential impacts in downstream Assam state. Anti-dam activists are now concerned Modi's government will now push ahead with a series of mega dams planned in the northeast region, ignoring all expert and advisory committees in an attempt to harness "green" hydropower.
The Dibang is just one of 168 massive dams slated to produce 57,000 megawatts of hydropower in Assam and Arunachal Pradesh states. This strategically important region, which borders Myanmar in the east, Bhutan in the west and China in the north, is described by politicians as India's 'future powerhouse' and is a key focus point of the country's dam building programme.
China is involved in a major dam building programme on its side of the border, also using the waters of the Brahmaputra – which it calls the Yarlung Tsangpo. China's plans to build a massive dam three times the size of the Three Gorges Dam on the Great Bend before the river swings round into India.
The Brahmaputra is one of the world's major rivers, winding across the Tibetan Plateau through China, India and Bangladesh before joining with the Ganga and flowing out into the Bay of Bengal.
The dam building programme in north-east India has been highly controversial. Opponents say it not only ignores geological and ecological factors – it also fails to take into account the impact of climate change in the region.
Experts also say no proper overall plan has been put in place: though India and China have signed a limited agreement to data on river flow, there is no specific deal on managing the Brahmaputra's waters.
Protests about the dams have been growing, with work on the 2,000 megawatt Lower Subansiri dam on one of the Brahmaputra's tributaries – repeatedly held up.
In the build up to this year's elections, Narendra Modi held a rally in Pasighat in the East Siang district of Arunachal Pradesh in February where he acknowledged peoples' concerns about large dams and committed to developing small hydropower instead.
"I know citizens of the region are against large power projects," he said. "I respect your sentiment. But hydropower can also be harnessed using smaller projects, while protecting the environment."
Chandan Kumar Duarah is a science journalist based in North East India.


Two Images, in particular, one showing Mahatma Gandhi dancing and the second claiming to be the real photograph of Godse shooting Gandhi surfaces mainly during both Birth and Death anniversary of Mahatma Gandhi for the past few years.
However, if the Image is looked carefully, one can easily spot the muscular built of the impersonator also Mahatma Gandhi always wore a specific leather footwear that was made by himself, which on observation can be easily spotted.
Now the second image is claimed to be taken moments before Mahatma was shot 30th January 1948, and circulated as the rare real photograph of Nathuram Godse.
The picture that is seen above is a shot from a 1963 British movie based on a novel by Stanley Wolpert named as "Nine Hours to Rama", here the character of Godse is played by a german actor "Horst Buchholz" and Mahatma Gandhi Character is played by Js Casshyap.
On careful observation, one can easily spot the difference between reel actors and real-life persons.
FactCrescendo verifies both the Images and narratives shared on various social platforms as fake and advice its readers not to believe in such propaganda's.


Target pom poms, Choose your outside colour first and then your inside colour second. These pom poms only work well on a dowel handle or a loop or block handle. Baton handle creates the target to be at both ends. Pom poms are not sold as a pair, priced individually.


text:
Initial Outfitters Launches Innovative New Mobile App from Krato
by Tol Bayo | Jan 23, 2018 | English Media Releases
New Initial Outfitters App Gamifies the Direct Selling Experience, in Order to help Consultants succeed
USA, 22 January 2018 -- SCOTTSDALE, AZ – Jan 22nd, 2018 – Founded in 2006, Initial Outfitters saw an opportunity to do something radically different – to teach others how to start their own personalization business through a supportive community. Today, Initial Outfitters has the privilege of working with thousands of amazing women who have a passion for something to call their own.
Through their work, they had a realization: their sales force, called Outfitters, needed a better way to start their new businesses quick. They discovered Krato, and together they created a new Initial Outfitters App that would engage the consultants with data, coaching, relevant messaging and a gamified path to success. The app presents Outfitters with specific actions that they can take to grow their own Initial Outfitters business, which makes the start-up process much easier.
The Initial Outfitters mobile app makes the sometimes daunting task of building a business much more manageable. Since the app cuts goals into smaller, easier tasks, Outfitters can have fun instead of stressing about what direction they should go in.
"We believe we exist to make a significant and lasting difference in the lives of our consultants and to truly make a difference, we must continue to innovate with tools to support their businesses", says Initial Outfitters Founder Alicia Storbeck.
Added Co-Founder Beth Reeves, "recognition is very important at Initial Outfitters and our new app opens up fresh opportunities for that."
Matt Lind, COO at Krato remarked, "Krato was honored to see the incredible reception that the Initial Outfitters organization had for the new Mobile App. It's an honor to be a partner with the Initial Outfitters team to increase engagement and success."
The direct selling industry is booming and generates billions in sales per year. The Initial Outfitters App—powered by Krato's Journey—will help consultants stay engaged and increase their success. The Mobile app is currently available on both iOS and Android devices.
About Initial Outfitters
Initial Outfitters is growing around the country as a result of the entrepreneurial spirit of their sales force, called Outfitters. The company has an amazing array of personalized jewelry and gifts, however, it is what those products do for people that makes the difference in their success.
Initial Outfitters lives it mission by giving back though the IO Loves program. Because of the hard work and gratitude of Initial Outfitters consultants and customers, Initial Outfitters has funded one million lunches for impoverished children around the world. Find out more at https://www.initialoutfitters.com/
About Krato
Krato.com is an organization that offers innovative mobile solutions to direct selling companies. Their vision is to bring direct selling to a new level with advanced mobile technology. Not only do Krato's tools help keep distributors on task; they help boost revenue by increasing customer engagement. For more information on Krato and what they have to offer, visit http://www.krato.com/


text:
"One for the Road" - czwarty singel brytyjskiego zespołu Arctic Monkeys promujący ich piąty album AM. Wydany 9 grudnia 2013. Na płycie winylowej 7" znajduje się także B-side zatytułowany "You're So Dark".

Przypisy

Linki zewnętrzne
 Okładka

Single wydane w roku 2013


I have seen the blendtec twister jar and vitamix making awesome nut butters, grinding coffee etc. Now why wouldn't you use such a thing for pregrinding the cocoa beans? For example it takes only a couple of seconds to grind coffee. I am very curious and looking forward to your answer.
Hi everyone and welcome to One year of Ask the Alchemist. Please keep those questions coming to question@chocolatealchemy.com.
The basic answer to this is it does not work. In theory it should (since they do nut butters) , but in practice it does not. In both cases I found the nibs would start to liquefy and then simply stick to the container wall, and all grinding would stop. The blades are not suited to keeping the material in play. And in the Vitamix's case, that amazingly strong motor caused so much friction it literally burned the chocolate.
As for it taking only a couple seconds to grind coffee….well, the same can be said for both blade grinders and burr grinders and neither work for cocoa due to the high cocoa butter content. It sticks to the side of the blade grinder and clogs the burr grinder. So it really does not matter that a given piece of equipment will do coffee well. Cocoa isn't coffee. As for the nut butters, the crux here is that the fats in nut butters are oils, i.e. liquid at room temperature. As they start to grind, they immediately fall back into the blades and it's all good. This doesn't happen with the cocoa since the cocoa butter is solid at first.
I'm keeping this short today as I have a bunch of cocoa to get tasted and up. Look for them tomorrow.


I got up early today, eager to start my new age. The day's okay not so grand but not ordinary as well. There were people I didn't expect to greet me but they did anyway. My computer even 'healed' itself and decided to grant me a 'sound' for my birhtday (it has been mute for days).
– I'm loud but not obnoxious, maybe because I laugh my heart out every time.
– I can easily change my mood.
– I'm easy to please.
– I am friendly. I might come a bit intimidating and snobbish but really I'm not.
-I AM A DREAMER. I DREAM BIG.
Being 26 is a whole new journey for me to take on but I'm ready come what may. I have my God, my family and friends, what more can I ask?


Are you struggling with bulimia?
Do you feel physically tired & mentally exhausted?
The key to overcoming bulimia is to learn how to control two main areas of your thinking.
I'm sure you've experienced hopeful thoughts about recovery, only to have these thoughts and hopes dashed by anxious thoughts about what if I feel better, but then it gets worse again? What will happen if that happens? All those thoughts running through your mind are hard to compete with – let's get them under control.
Secondly, we will develop skills to help you learn how to manage your expectations. Most people with bulimia are either experiencing it's problematic thoughts and behaviours right now, or experiencing a brief respite, yet expecting it to rear it's head at some point soon – there is very often a "fear of the fear" which comes along with the peaks and troughs bulimia creates.
We'll neutralise that line of thinking, and give you a solid foundation to adopt more realistic thinking instead.
If you are struggling with bulimia, I genuinely believe CBT Therapy is a useful way to help you overcome your concerns. CBT isn't about digging up the past and endlessly analysing it and looking intrusively for hidden meaning.
If My approach is as unique as the person I work with, so I'll find the right way to work with you. Perhaps you would like to begin slowly? Just want to talk on a general level about your concerns and experiences around bulimia and get things off your chest – no problem.
Bulimia can be overcome. Whilst learning to change isn't always simple and straightforward, it certainly can be done with time, effort and determination.
Take action, you've struggled long enough. Don't spend any more time hoping that it will just go away all by itself – it probably won't.
The sooner you seek help, the sooner you will learn how to overcome it and future proof your results.


text:
Kindness is a language which the deaf can hear and the blind can see
Plant a tree to help us breathe... Buildings, buildings everywhere and no fresh air to breathe... Plant more trees, make environment pollution free... Save trees now, they will save us in future... Trees give good look, don't destroy them from earth book... Save a tree to help us breathe... Plant trees in your neighbourhood
Home Our Mission
Join Us Events/Functions Organised Articles/Stories Publications Contact
Saving Nature and Mankind
I take privilege to introduce "We The Human" as a public charitable trust registered on 13th Feb 2018, under Indian Trust Registration Act 1882, presently functioning from its registered office at #15/01, Ground Floor, Punjab National Bank Building, (Road Opp Jammu Darbar) Channi Himmat, Jammu Pin Code: - 180015 Jammu & Kashmir, e-mail: wethehuman@gmail.com, Web: www.wethehuman.in
Main focus of the trust is to work for generating awareness & taking up activities for restoration of degraded human values and damages caused to our environment because of unsustainable growth & development as well as due to side effects of "Green Revolution", witnessed by all of us, during last three - four decades. Ecological balance of soil of earth is badly disturbed due to excessive use of toxic & synthetic chemical fertilizers, insecticides, pesticides, herbicides, fungicides which have contaminated our water bodies, as well.
Perhaps, many among us are not aware about Abohar-Jodhpur passenger train which is named by locals as Cancer Train, running between Bathinda and Bikaner, daily, Patients undergo almost free of cost treatment at the Acharya Tulsi Regional Cancer Treatment and Research Centre (RCC) Bikaner. Punjab's Malwa region's (the state's cotton belt) areas viz., Bathinda, Faridkot, Moga, Muktsar, Ferozepur, Sangrur & Mansa known together as the Malwa region, farmers and families including infants here are grappling with cancer and other serious health problems that have crept into their homes through the backdoor as the farmers of India's grain bowl fed the nation. Many members of these farmers' families died of cancer in the past decade and thousands of others are under treatment. Experts say this "cancer train" symbolizes the adverse impact of the "Green Revolution" on public health in Punjab. The lush fields in this region hide a scary tale; Farmers live in a disturbing cesspool of toxicity, as a result of excessive and unregulated use of pesticides and chemical fertilizers. Reportedly, Punjab farmers' use of pesticides is approximately 923 g/ha, way above the national average of 570 g/ha (grams per hectare). Same situation is going to happen in our state as well; since a number of varieties of vegetables are being produced in agricultural farms near river banks, by having heavy sprays of toxic & synthetic chemical insecticides, pesticides, fungicides, herbicides, plant growth regulators directly on vegetables crop. The same vegetables, with chemical residues, are consumed by locals as well as exported to outside state. People of J&K are witness of upsurge in cancer patients in the state, at an alarming level, during last five to seven years. "We The Human" team has launched an initiative to generate awareness among people, agricultural farmers as well as government authorities about health hazards as well as harm to earth soil due to heavy sprays of toxic & synthetic chemicals directly on the crop. A delegation comprising of "We The Human" founder members met Sh. Raj Nath Singh Union Home Minister at his residence in New Delhi on 25th December 2017and submitted a memorandum, on this as well as other similar serious issues.
Similarly, Indian society is, today, passing through a transit period from illiteracy, fanaticism, caste/ religion based policies, gender based discrimination, unemployment and old values to a new, modern India. Crime against women, female foeticides, child abuse, unemployment, communal hatred & religion based conflicts, land mafias, conversion of agricultural land into residential colonies, terrorist activities, migration of local population to urban or semi urban areas out of fear or in search of better employment avenues, increase in diseases due to sedentary life styles, mental health, insufficient health facilities, insufficient supply of clean drinking water, insufficient irrigation facilities, organized street beggars are few among other trouble areas in present India, "We The Human" have decided to take up simultaneously.
A major challenge recently emerged before humanity in our state as well as India, is about disposal of bio-degradable as well as non bio-degradable garbage and bio medical wastes. Recently a three-Judge Bench comprising Justices Madan B Lokur, Kurian Joseph and Deepak Gupta of Supreme Court of India had asked Additional Solicitor General A. N. S Nadkarni, representing GoI, "Are the centre, states and UT governments in India waiting for the atom bomb of garbage to explode?" There is not only need for governments in states & UTs to take concrete measures, but also for the common public to change its mindsets about disposal of household as well as other garbage/ waste. For that, we have taken an initiative, in J&K, by launching aggressive campaign on this issue.
Our few founder members, with the help of few senior newspaper journalists, have been successful in stopping practice of using carcinogenic calcium carbide for artificial ripening of fruits like papaya, mangoes and banana in Fruit & Vegetable Markets Narwal Jammu and Parimpora Srinagar by raking up this sensitive issue and putting pressure on traders as well as the concerned government authorities with intervention of J&K High Court, by filing a Public Litigation Interest petition to this effect.
Our trust has a large number of members from different sections of society including businessmen, entrepreneurs, technocrats, educationists, bureaucrats, advocates, journalists, photographers, social & peace workers. Apart from this, we believe in maintaining good liaison with Panchayat members and people in rural areas. We provide and arrange for platforms/ forums, where stake holders of agriculture sectors are invited for workshop cum practical demonstration of composting/ integrated & sustainable system of farming, by resource persons/ demonstrators as well as senior functionaries of the government and environment activists.
Apart from this, one sixteen to twenty pages newsletter on "Nature & Humanity" is being released, every month, which is used as IEC material, apart from other such material, and are being circulated, free of cost, among our members, participants in events/ workshops organized by "We The Human" and other institutions like Universities, Colleges, Airports within the state and foreign embassies' offices, located in New Delhi. Awards of honor are distributed, by "We The Human" among those having achievements & exemplary contributions for environment and social/ human cause.
We seek your valuable support into this noble endeavor initiated by a group of likeminded & selfless people, dedicated purely towards nature and mankind.
Soliciting your help and guidance
(Sh. Ram Saroop Gupta)
29-11-2021 -> COVID-19 relief material handed over to Chairman Health & Sanitation JMC
30-09-2021 -> People need adhere to CAB to avoid third wave of Corona: K Y S Manhas
20-09-2021 -> First Skill Development Centre, sponsored by We The Humans and Swasthya Evam Shiksha Charitable Trust Delhi
29-08-2021 -> Plantation drive at Samba; K Y S Manhas demands government environmental task force in J&K
17-08-2021 -> We The Humans extend helping hand to family in harness
25-07-2021 - We The Humans launch plantation drive at Samba
25-06-2021 -> We The Humans distribute free ration among 60 families in Samba district
Single use plastic choking our planet - Article by Anuj.K.Verma
All Rights Reserved - Designed & Maintained by
Bhan Technologies
Site Best Viewed in 1024 x 768 Resolution


text:
Q: Instrumenting a Java Application With Prometheus I am trying to  monitor multiple metrics in a java application using the prometheus java client library but I am having difficulties monitoring more than one metric at a time. If I register and scrape only one metric everything seems to work fine and I can see the metric using the prometheus application but if I try to expose and monitor more than one metric then only one of the metrics will be visible.
So If I have an application with the instrumented class as below
package com.telemetryserver.Instrumentation;

import io.prometheus.client.*;

import javax.servlet.ServletException;
import javax.servlet.http.HttpServlet;
import javax.servlet.http.HttpServletRequest;
import javax.servlet.http.HttpServletResponse;
import java.io.IOException;

public class Instrumented_Class extends HttpServlet
{
    private static Instrumented_Class _instance = null;

    public static final Gauge metric_1 = Gauge.build().name("metric_1").help("metric_1").register();
    //public static final Gauge metric_2 = Gauge.build().name("metric_2").help("metric_2").register();

    public static Instrumented_Class getInstance()
    {
        if (_instance == null)
            _instance = new Instrumented_Class();

        return _instance;
    }

    //Getters and Setters

    public static void setMetric_1(double val) { metric_1.set(val); }

    public static double getMetric_1() { return metric_1.get(); }

    //public static void setMetric_2(double val) { metric_2.set(val); }

    //public static double getMetric_2() { return metric_2.get(); }

    @Override
    protected void doGet(final HttpServletRequest req, final HttpServletResponse resp) throws ServletException, IOException
    {

        double metric_1 = Instrumented_Class.getMetric_1();
        Instrumented_Class.setMetric_1(metric_1 + 1);

        //double metric_2 = Instrumented_Class.getMetric_2();
        //Instrumented_Class.setMetric_2(metric_2 + 2);

        resp.getWriter().println("Hello from Instrumented_Class!!!," +
                " metric_1 = " + metric_1
                //+ " metric_2 = " + metric_2
        );
    }
}

and the main class as
package com.telemetryserver.client;

import com.telemetryserver.Instrumentation.*;
import io.prometheus.client.exporter.MetricsServlet;
import io.prometheus.client.hotspot.DefaultExports;
import org.eclipse.jetty.server.Server;
import org.eclipse.jetty.servlet.ServletContextHandler;
import org.eclipse.jetty.servlet.ServletHolder;

public class TelemetryApp
{
    public static void main(String[] args)
    {
        startTestServer(2018);
    }

    public static void startTestServer(int port)
    {
        try
        {
            Server server = new Server(port);
            ServletContextHandler context = new ServletContextHandler();
            context.setContextPath("/");
            server.setHandler(context);

            //Expose our Instrumented servlet.
            context.addServlet(new ServletHolder(Instrumented_Class.getInstance()), "/");

            //Prometheus Metrics Servlet
            context.addServlet(new ServletHolder(new MetricsServlet()), "/metrics");

            // Add metrics about CPU, JVM memory etc.
            //DefaultExports.initialize();

            // Start the webserver.
            server.start();
            server.join();
        }
        catch (Exception ex)
        {
            ex.printStackTrace();
        }
    }
 }

and also using the YAML file
global:
  scrape_interval:     2s # Set the scrape interval to every 15 seconds. Default is every 1 minute.
  evaluation_interval: 2s # Evaluate rules every 15 seconds. The default is every 1 minute.
  # scrape_timeout is set to the global default (10s).

# Alertmanager configuration
alerting:
  alertmanagers:
  - static_configs:
    - targets:
      # - alertmanager:9093

# Load rules once and periodically evaluate them according to the global 'evaluation_interval'.
rule_files:
  # - "first_rules.yml"
  # - "second_rules.yml"

# A scrape configuration containing exactly one endpoint to scrape:
# Here it's Prometheus itself.
scrape_configs:
  # The job name is added as a label `job=<job_name>` to any timeseries scraped from this config.
  - job_name: 'telemetryserver'

    metrics_path : '/metrics'
    # scheme defaults to 'http'.

    static_configs:
    - targets: ['localhost:2018']

I can monitor the metric "metric_1" by running prometheus and then running the query "metric_1". This is all ok, but if I add another metric called metric_2 by uncommenting the lines in the instrumented class then metric_2 shows up on prometheus but metric_1 stops showing up. Further more, if I uncomment the DefaultExports.initialize(); line (which adds all the default prometheus metrics) then both metric_1 and metric_2 both stop showing up and only a default metric called "jvm_threads_current" shows up.
Can someone please enlighten me on how I can monitor multiple metrics in prometheus? Any help appreciated.

A: Your application is fine.
Just make sure to test it by hitting the /metrics endpoint. This is how prometheus collects the metrics from your application.
When I test it locally, with both metrics and exporting the JVM metrics, I have an output similar to this one:
*** lots of jvm metrics
jvm_gc_collection_seconds_count{gc="PS MarkSweep",} 0.0
jvm_gc_collection_seconds_sum{gc="PS MarkSweep",} 0.0
# HELP metric_2 metric_2
# TYPE metric_2 gauge
metric_2 12.0
# HELP metric_1 metric_1
# TYPE metric_1 gauge
metric_1 6.0
# HELP jvm_threads_current Current thread count of a JVM
# TYPE jvm_threads_current gauge
jvm_threads_current 16.0

Which contains your custom metrics and also the metrics from JVM.
Your Prometheus configuration is also ok. To test the integration, go to "Status">"Targets", and you should see your service there like in the image bellow:

Then go to "Graph" page and enter the expression {job="telemetryserver"} to check that Prometheus is in fact collecting the metrics from your application. You should see something similar to:

redpajama_set_name: RedPajamaStackExchange


Business Connexion (BCX) CEO Benjamin Mophatlane, 41, has died.
"The Business Connexion group board and exco announces with profound regret and sadness, the passing of our chief executive officer, Benjamin Mophatlane," the company said in an internal e-mail.
"Benjamin was in a meeting with Telkom yesterday afternoon in Rosebank when he complained of chest pains and subsequently had a cardiac arrest," it said.
BCX is currently the subject of a R2,7bn takeover bid by Telkom.
Mophatlane had celebrated his 41st birthday just a month ago.
"In his career as our deputy CEO and later as our CEO he left an indelible mark on each one of us, our company and the South African business landscape at large. He was instrumental in building Business Connexion into South Africa's leading IT company.
"The board and exco, and we think we speak for each and every one of us, when we say we would like to honour Ben for his leadership, vision, integrity and esteemed contribution to the company and we all express our heartfelt sympathy and condolences to his wife Abby and their three children, his mother, sister and twin brother Isaac.
"Ben will be remembered for his undeniable entrepreneurial spirit, enthusiasm and exceptionally warm personality, which are aspiring attributes for anyone.
In 1996 the Mophatlane twins co-founded what was then known as Business Connection. Business Connection subsequently merged with Seattle Solutions in 2001, with Benjamin serving as MD until the company merged with Comparex Africa in 2004 and was renamed Business Connexion.
He served as deputy CEO until 2007, when he became CEO.
The company said it will make further announcements in due course.


text:
<?php
if ( !defined( 'DC_CONTEXT_ADMIN' ) ) { return; }

// Process the POST data.
try {
  if ( !empty( $_POST ) ) {
    // `enable` setting.
    $core->blog->settings->postsStats->put( 'enable',
      !empty( $_POST['enable'] ), null, null, true, true );

    // `enable_view_count_antiflood` setting.
    $core->blog->settings->postsStats->put( 'enable_view_count_antiflood',
      !empty( $_POST['enable_view_count_antiflood'] ), null, null, true, true );

    // `view_count_antiflood_expiry` setting.
    $core->blog->settings->postsStats->put( 'view_count_antiflood_expiry',
      (integer)$_POST['view_count_antiflood_expiry'], null, null, true, true );

    // Popularity coeff settings.
    foreach ( array( 'view', 'comment', 'share' ) as $type ) {
      $popularityCoeff = is_numeric( $_POST[$type . '_popularity_coeff'] ) ?
        (integer)$_POST[$type . '_popularity_coeff'] : -1;

      if ( $popularityCoeff >= 0 ) {
        $core->blog->settings->postsStats->put( $type . '_popularity_coeff',
          $popularityCoeff, null, null, true, true );
      }
    }

    // Social networks check setting.
    foreach ( PostsStatsHelper::getSupportedSocialNetworks() as $name => $site ) {
      $core->blog->settings->postsStats->put( 'check_' . $site,
        !empty( $_POST['check_' . $site] ), null, null, true, true );
    }

    // `share_count_caching_expiry` setting.
    $core->blog->settings->postsStats->put( 'share_count_caching_expiry',
      (integer)$_POST['share_count_caching_expiry'], null, null, true, true );

    // Facebook app access token.
    $core->blog->settings->postsStats->put( 'facebook_app_access_token',
      $_POST['facebook_app_access_token'], null, null, true, false );

    dcPage::addSuccessNotice( __( 'postsStats settings have been successfully updated.' ) );

    // Required to reflect the changes.
    http::redirect( $p_url . '&up=1#postsStats-settings' );
  }
}
catch ( Exception $e ) {
  $core->error->add( $e->getMessage() );
}
?>

  <title>postsStats</title>

  <?php echo dcPage::jsPageTabs(); ?>

<?php
echo dcPage::breadcrumb( array(
  __( "Plugins" ) => '',
  'postsStats' => ''
) );

$settings = $core->blog->settings->postsStats;
$socialNetworks = PostsStatsHelper::getCheckedSocialNetworks( $core );

// Populate the values to feed the HTML forms with.
$expiryTimes = array(
  '30 sec' => 30,
  '1 min' => 60,
  '5 min' => 300,
  '10 min' => 600,
  '15 min' => 900,
  '30 min' => 1800,
  '1 h' => 3600,
  '6 h' => 21600,
  '12 h' => 43200,
  '24 h' => 86400
);

// Create the HTML forms.
if ( $settings->enable ) {
  echo(
    '
' .
    '
' .
    '
Entries list
' .
    '
' .

    '
' .
    'Title	' .
    'Date	' .
    'Comments	' .
    'Views	' .
    'Unique views	' .
    'Shares	'
  );

  foreach ( $socialNetworks as $name => $site ) {
    echo( '' . $name . '	' );
  }

  echo(
    'Popularity	' .
    '
'
  );

  $posts = PostsStatsHelper::fetchAll( $core, array( 'no_content' => true ) );
  while ( $posts->fetch() ) {
    echo(
      '
post_id . '">' .
      'post_id .'">' . $posts->post_title . '	' .
      '' . dt::dt2str( __( '%Y-%m-%d' ), $posts->post_dt ) . '	' .
      '' . $posts->nb_comment . '	' .
      '' . $posts->nb_view . '	' .
      '' . $posts->nb_unique_view . '	' .
      '' . $posts->nb_share . '	'
    );

    foreach ( $socialNetworks as $name => $site ) {
      echo( '' . $posts->{'nb_share_' . $site} . '	' );
    }

    echo(
      '' . $posts->popularity . ' (' . round( $posts->relative_popularity * 100, 0 ) . '%)	' .
      '
'
    );
  }

  echo(
    '
' .
    '
' .
    '
'
  );
}

echo(
  '
' .
  '<form action="' . $p_url . '" method="post">' .

  '
' .
  '
' . __( 'General' ) . '
' .

  '
' .
  form::checkbox( 'enable', 1, $settings->enable ) . ' ' .
  __( "Enable postsStats" ) .
  '
' .

  '
' .
  form::field( 'view_popularity_coeff', 6, 6, $settings->view_popularity_coeff ) . ' ' .
  __( "Popularity coefficient for each unique post view" ) .
  '
' .

  '
' .
  form::field( 'comment_popularity_coeff', 6, 6, $settings->comment_popularity_coeff ) . ' ' .
  __( "Popularity coefficient for each post comment" ) .
  '
' .

  '
' .
  form::field( 'share_popularity_coeff', 6, 6, $settings->share_popularity_coeff ) . ' ' .
  __( "Popularity coefficient for each post share" ) .
  '
' .

  '
' .
  '
' .
  '
' . __( "Anti flood" ) . '
' .

  '
' .
  form::checkbox( 'enable_view_count_antiflood', 1, $settings->enable_view_count_antiflood ) . ' ' .
  __( "Prevent a visitor from increasing the unique view count of " .
    "a same post within a certain lapse of time" ) .
  '
' .

  '
' .
  __( "Lapse of time in seconds:" ) .
  ' ' . form::combo( 'view_count_antiflood_expiry', $expiryTimes,
    $settings->view_count_antiflood_expiry ) .
  '
' .

  '
' .
  '
' .
  '
' . __( 'Social networks share count' ) . '
' .

  '
' .
  form::checkbox( 'check_facebook', 1, $settings->check_facebook ) . ' ' .
  __( "Check Facebook" ) .
  '
' .

  '
' .
  form::checkbox( 'check_googleplus', 1, $settings->check_googleplus ) . ' ' .
  __( "Check Google+" ) .
  '
' .

  '
' .
  form::checkbox( 'check_linkedin', 1, $settings->check_linkedin ) . ' ' .
  __( "Check LinkedIn" ) .
  '
' .

  '
' .
  form::checkbox( 'check_pinterest', 1, $settings->check_pinterest ) . ' ' .
  __( "Check Pinterest" ) .
  '
' .

  '
' .
  form::checkbox( 'check_twitter', 1, $settings->check_twitter ) . ' ' .
  __( "Check Twitter" ) .
  '
' .

  '
' .
  __( "Lapse of time in seconds before the share count should not return the cached value anymore:" ) .
  ' ' . form::combo( 'share_count_caching_expiry', $expiryTimes,
    $settings->share_count_caching_expiry ) .
  '
' .

  '
' .
  '
' .
  '
' . __( 'Facebook access token' ) . '
' .
  '
' .
  __( 'If this website is hosted on a shared server and another user makes too many requests
  to the Open Graph API, you might not be able to retrive the number of shares for Facebook.
  To workaround this issue, create a Facebook App and copy its access token below.' ) .
  '
' .

  '
' . form::field( 'facebook_app_access_token', 32, 128, $settings->facebook_app_access_token ) . ' ' . '
' .

  '
' .

  '
<input type="submit" value="' . __( "Save" ) . '" />' . $core->formNonce() . '
' .

  '</form>' .
  '
'
);
?>

redpajama_set_name: RedPajamaGithub


text:
Delores Laura Wohlk
Delores Laura Wohlk, 87, of Almena passed away, March 13, 2020 at Care and Rehab-Cumberland. She was born April 16, 1932 in Waverly, IA to Irven and Bertha (Tegtmeier) Gabe. At the age of 27 she moved to Almena where she met her soon-to-be husband. Delores married Calvin Wohlk on May 7, 1960; he preceded her in death on September 26, 2012.
She and Calvin dairy farmed until they retired. She was a member of the St. Matthew Church in Almena, and an active member of Ladies Aid. Delores served on the Altar Guild for over forty years; was a 4H Leader, and was a member of the Turtle Lake American Legion Auxiliary.
Delores was a talented cook, gardener, and enjoyed crafts.
Delores is survived by her son, Glen (Susan) Wohlk of Almena; two daughters, Hollis "Holly" (Brad) Nyhus of Cumberland, and Karla (James Jr.) Mattson of Chetek; eight grandchildren: Clayton, Bethany, and Kurt, Darren, Travis and Eric, Adam and Niccole; four great grandchildren: Cora, Aria, Michael, and Elara; brother, Ronald (Beverly) Gabe of Almena; nieces, nephews, cousins and other relatives and friends. She was preceded in death by her husband, Calvin; and an infant brother, Leon.
Funeral services were held at 11:00 a.m. Wednesday, March 18, 2020 at St. Matthew Lutheran Church, Almena, WI with Pastor Preston Paul officiating. Burial was in St. Matthew Cemetery, Almena, WI. Pallbearers were Clayton Wohlk, Bethany Bird, Kurt Wohlk, Darren Nyhus, Travis Nyhus, Eric Nyhus, Adam Mattson, and Niccole Mattson.
Skinner Funeral Home of Cumberland is serving the family.


This item is a used combo column switch assembly w/ fog lamps for a 2013 Nissan Altima. The part numbers are 25560-3TP1A and 255603TP1A. This OEM Genuine Nissan part sells for $75 and comes with a warranty.