YES. Supporting rural business is really paying off.
Is a 'hot desk' in the countryside ostensibly a wonky little table set up in a pungent straw-strewn farmyard in deepest Shropshire in blazing August? Erm, no. And 'hot desking' isn't even the right name for shared office space anymore! Not really. These days, it's all about cool and SO on-trend desk sharing spaces, made available to the self-employed, start-ups, small companies, SMEs, or even directly employed people.
Shared office space provided for business professionals from a broad range of industries is massively popular in London, Manchester, Birmingham, Newcastle, Glasgow, Belfast and just about every British city; and it's becoming all the rage in rural areas, with many rural coworking hubs already being well-established. That's great for all types of business professionals without fast and easy access to cities and large towns, and it's making a considerable contribution to rural economies, east, south, north and west.
Living the Good Life in a pastoral idyll is a fantasy that many of us have entertained at some point or another, isn't it? Leaving all the stresses and strains of city life behind and heading out to rustic bliss can feel almost dreamlike, as images are quickly conjured in the mind of picture postcard cottages, cream teas, family walks with the dog, tasty ploughman's lunches in The Drunken Duck beside the river... and then an unhurried stroll home down winding country lanes, well in time for Emmerdale. Working in a shared office space in a rural area means that you can realise the dream of escaping the daily punishing commute into the city, whilst simultaneously thriving in the professional business world. Wow. Who'd have ever thought it? And, if you already live in a rural area, like so many other professionals, working at your home office a couple of days per week, and sometimes building your business as a coworker at a location where there's a lovely sense of community and inclusion, could prove ideal.
A huge problem for so many self-employed professionals is feeling unable to cope with the seclusion of working alone, all day, every day. It can drive even the most determined start-up warrior back to the directly employed workforce, in fact. By opting instead to work in a shared office building – a dedicated contemporary facility, purpose-built to meet the needs of committed professionals (and maybe the odd creative dreamer!) – you slay the isolation monster in one stroke. Whilst benefiting from gratis car parking, get access to a shared internet connection (usually free Wi-Fi), refreshments, a desk space as a dedicated area to work, and maybe a printer with photocopying also being an option, during business hours (and at some rural locations, 24/7), and love the fact that the whole deal is entirely affordable too.
All this is helping professionals to keep costs down as they look to network and grow their businesses, whilst simultaneously boosting the rural economy, which – no longer being primarily reliant upon traditional farming and other agricultural practices – needs to always find ways to remain sustained financially. Research is undertaken by think-tanks and rural affairs-focused groups, such as the Centre for Rural Economy in the North East (CRE), for example, has revealed that the growing popularity of shared office spaces in country areas is more than playing its part in generating revenue for a broad range of businesses. This is leading to strengthened stability and increased prosperity for all. In fact, Britain's rural areas have reached a point now where their regional economies claim that desk sharing's "the new sheep shearing!"


This session has been waiting to be published for a couple of months and I could not be more excited to share it with you. I had the pleasure of filming Chatham County Line from Rayleigh, North Carolina. To celebrate the release of their seventh studio album Autumn, here's the session we filmed in LantarenVenster, Rotterdam earlier this year. Expect bluegrass of the highest quality and performances that are infectiously fun.
I am very happy that I stuck around after the session to watch them play live that night. Rarely have I seen an acoustic show that entertained the entire audience for that long. They showed true showmanship, plus they have the skills to back it up. I remember sending a message to a friend that night saying it's like listening to bluegrass versions of The Band songs. The great thing about Chatham County Line is that they combine the typical bluegrass sound with a more traditional element and an impeccable songwriting talent. Everything about this band just seems to work.
(…) We have never strived to be anything other than genuine in what we do. It's a good feeling when so many appreciate what we have worked so long and hard to achieve.
Earlier this year the band – who've had the flag of North Carolina hanging on stage during every show for 16 years – took down the flag in protest of the ridiculous HB2 law, stating: "Heartbreaking, but this comes down until #HB2 does." It resulted in a lot of short sighted, sometimes questionable messages on social media which is pretty stupid, because there aren't many bands that I've met who are as proud and speak so fondly of their hometown as Chatham County Line.
But, getting back to the music: It's really good that with Autumn there now is a successor to Tightrope, as that record has been played A LOT over here in the Onder Invloed office. You try watching them play Should Have Known and not end up singing it to yourself for days to come. Just try it. See? I told you.
LantarenVenster in Rotterdam, The Netherlands.


text:
Earlier this year, Special Collections launched its first remote internship for University of Exeter students. Unable to run our usual in-person work experience programme, and knowing that another lockdown at the start of 2021 was highly likely, we were pleased to offer an opportunity for students to gain valuable archive experience whilst working from home.
The collection we chose for this remote internship was the Letters of John Jarmain (EUL MS 413). William John Fletcher Jarmain (1911-1944) was a novelist and poet. He served throughout the Second World War as a gunnery officer with the 51st Highland Division during their campaigns in North Africa and Sicily. He took part in the D-Day landing and was killed in action on 26 June 1944. The collection comprises 120 manuscript letters that he sent home to his wife Beryl between June 1942 and November 1943.
EUL MS 413/1/66 – Aerogram dated 10 March 1943
Digitised images of all of the letters are available to view online through our Digital Collections website, making them ideal for our interns to access and transcribe from home. Once proofread, the transcripts produced by the interns on this project will be uploaded to the website to sit alongside the digitised letters, enabling letters of interest to be more easily identified, accessed and understood.
We would like to take this moment to thank our interns, Beth Howell and Ruby, for their hard work, diligence and enthusiasm for this project. Through a combined effort, they recently completed the transcription of all 120 letters – an amazing achievement! Below you can read their reflections on the project.
Reflections by Beth Howell
Transcribing the letters of a person is always a very involved experience, and working on John Jarmain's war-time correspondence has proven to be no exception. However, perhaps because Jarmain was so engaged with the process of writing, (often demonstrating himself to be an almost obsessive editor of his own poetry), he always seems to write with a real sense of how his words might be read and interpreted in the future, making his letters a real privilege to read. Though most of his correspondence is addressed to his wife, Beryl, he often appears to imagine a reader beyond her, documenting the world around him with a real sense of capturing the present moment. His letters are therefore not only interesting because of what they reveal about his poetic practice, but also the landscapes he found himself in, the relationships he fostered, and his hopes and anxieties for a future after the war.
My favourite element of Jarmain's writing, though, was probably the way in which he balanced larger concerns with little details. His ability to find joy in the spaces around him, even though the vision of those landscapes necessarily meant his separation from home (and, of course, were imbued with the ever-present anxieties of potential battles), is really heartening and beautiful to read. He loved birds, and many of his letters are preoccupied with identifying species from a little bird book he bought and carried around with him. (Though I have to say that deciphering rare specimens from his sometimes quite hastily-scribbled writing presented a few challenges- I had certainly never heard of a rufous warbler before!)
EUL MS 413/1/85 – Letter dated 30 April 1943, in which Jarmain writes about birds, including the rufous warbler (highlighted)
I also admired his confidence in informing his wife that he had fallen in (platonic) love with various women during his time in service- including Yone May, the subject of one of his poems. Jarmain presents a tangible picture of contemporary technologies (or quite the opposite), which affect his writing in a very material way- he finds himself scribbling in pencil, writing by candlelight in the wee hours, hastily penning an aerogram when he knows the post is leaving soon. He laments his ability to construct suitable diagrams of views and barracks, continues to marvel at unexpectedly quick postal deliveries, and to agonise when the opposite proves to be the case. His letters are a fascinating and absorbing insight into his life away- checked only by the knowledge that his observations would be tragically cut short. Jarmain died, killed by a fragment of mortar shell, on Saturday 26th June, 1944.
EUL MS 413/1/19 Letter dated 11 October 1942, next to transcript by Beth Howell
Reflections by Ruby
It hardly seems right to call this internship "work". Work refers to something laborious, something that has to be done, but I found transcribing John Jarmain's letters delightful. It saddens me that the World War II poets don't receive the same attention as the World War I poets. Jarmain, though brilliant and sensitive, is far from a household name and does not even have a poetry collection currently in print. This is what makes me so genuinely honoured to have been involved in this project, typing up his letters, so that we can start to make Jarmain's literature more accessible for more people. I hope that, going forward, people will read these letters and be touched in the same way that I was.
This internship has shown me that there is a big difference between reading for pleasure and reading to transcribe. Transcribing Jarmain's letters has forced me to read them carefully, sensitively and attentively. I have had to pay attention to punctuation, names and form which I might not otherwise have paid much attention to. When I've read letters from authors in the past, I don't tend to focus on people who are off-handedly mentioned (cousins, distant friends, colleagues etc.), and only really focus on those they are closest to. However, when writing up these letters I had to pay attention to every name — zooming in to make sure that I got every surname right — and, in doing so, I noticed certain people who popped up time and time again (his friend, Harry, for example). Jarmain's handwriting also means that it's easy to mistake a semicolon for an exclamation point. At first glance, his semicolons can look like exclamation points, but when you look more closely, they're usually not. If I were reading these letters at a glance, I would think that he was just heavy-handed with exclamation points, but this project showed me that he is not, and that he actually uses exclamation points quite sparingly. Over the course of the internship, I became more familiar with Jarmain's writing style and more attentive to quirks in his handwriting. For example, when writing "a", he tends to attach it to the word in front (i.e. if he says "a ship", he will write "aship"). This led to some tenuous guessing at the start of the project; however, I was familiar with this by the end, and found transcribing his letters much easier.
EUL MS 413/1/14 – Aerogram dated 28 September 1942, mentioning his friend Harry (highlighted)
The internship showed me how important it is to read letters attentively and slowly — to savour them and their images and their kindnesses. This is what Jarmain's wife, Beryl, would have done, and so we perhaps get closer to the experience of these letters when we read in this way. Having to read Jarmain slowly was probably my favourite part about and, as a consequence of having done this, I feel like I know him better than I otherwise would have done.
One particularly striking part of Jarmain's letters is just how little he refers to the actual events of war. He hardly talks about what his troops are doing, and any danger they might be in. Rather, he documents domestic experiences — for example, how he spent his time on leave, or how he goes swimming in the morning before starting work, or a joke told by one of the men. Jarmain separates himself from his identity as the "soldier" and presents himself as a real man, the same husband to whom Beryl waved goodbye. Though this is humbling to see, it also points to the separation between war and home which he documents in his poem 'El Alemein'. The separation between Jarmain as husband and soldier in these letters makes the dramatic irony of his death all the more upsetting. Reading the letters, I knew that he would never come home and safely settle back into domestic life. In one of his last aerograms (EUL MS 413/1/153), he writes of the Christmas presents he plans to give them, clinging to the possibility that the war will end soon and he will be home with Beryl and Janet-Susan. When the letters abruptly stop, there is no warning and, since he was so secretive about his life as a soldier when writing to Beryl, it seems strangely incongruous that he could have been killed in war.
EUL MS 413/1/33 – Aerogram dated 8 December 1942: draft of poems 'For Alamein'
Possibly my favourite parts to transcribe were his descriptions of nature — and, in particular, his descriptions of Italy in his final aerogram (EUL MS 413/1/154): "Away to the right, tier upon tier lit in streaks of sun and shade and clotted with white clustering towns, were the hills of Italy across the strait. In England you cannot imagine such beauty, such a scene". You can feel the wonder in his voice here and the sheer extent of the view he relays. These nature descriptions are occasionally shown in his poems, but only fleetingly, and I enjoyed reading this different writing style from him. It is also so illuminating to see the poems embedded within these letters because the poems will often refer to images he's already described for Beryl. For example, in letter one (EUL MS 413/1/1), he writes that he "was struck suddenly by willows, English willows, how they stand in rows like thick-handled powder-puffs, grey-green in the evening". Then, in a poem in letter two (EUL MS 413/1/2), he writes that the train "Passed willows greyly bunching to the moon". In this, we can see his poems as snapshots of real, personal experience. Indeed, the fact that they are embedded within letters shows just how intimate and personal they are, which can and should encourage us to read them contextually in new ways.
Ruby has very kindly recorded herself reading John Jarmain's first letter (EUL MS 413/1/1). Click on the play button below to listen to the recording.
https://specialcollections.exeter.ac.uk/wp-content/uploads/2021/07/Jarmain-1.m4a

redpajama_set_name: RedPajamaCommonCrawl


I have wanted to hike el Camino de Santiago de Compostela, the Way of St. James, ever since I read Pualo Cuelho´s book The Pilgrimage more than a decade ago. Now, with the publication of Walkabout Northern California: Hiking Inn to Inn this spring, I decided to give myself the gift of a 500 mile trek across northern Spain.
On the SF to Half Moon Bay hike walking on sand?
I plan to walk from SF Fort Funston to Half Moon Bay, as in the book. I have a few questions.
Was there a lot of walking on sand? If so, what shoes should be worn and were the tides a problem or dangerous?
Also, is there a good place meet up with people at Fort Funston (i.e. a parking lot or building that can be easily found with a car)?


Sheet lifter three hole. Rugged dependable Bradley Motorized Sheet Lifters are a sure way to boost hole your productivity. hole When frequent handling three is needed , when access three to the lifter is limited a motorized lifter is three the ultimate choice. Computerized Sewing Machine. Bail is designed to remain upright for easy attachment. Have a look on 4. Get Log Lifter Plans Now Free: Find three the right plan for lifter your lifter next woodworking project. lifter [ Query: ] I finally changed three plugs in my aluminum- head B230F to find that my number two plug hole was somehow cross- threaded by a previous mechanic ( the lifter plug was tough to remove the new plug tough sheet to install.
Feb 05, · A point of clarification: The 17/ 7. Yates® Dynamic hole hole Lifter® Soil Improver and Plant Food is ideal to use on hole all three garden plants. 2 at Worlds and a successful attempt of the same weight at the American Cup. Jan 11 the 409W engine, · We celebrate 50 years of the first big- block Chevy engines, their involvement in Chevrolet cars , the 348W engine , trucks as well as drag racing - Super sheet Chevy. Brother NV2600 Computerised Home sheet Embroidery hole Sewing Machine + Free PE Design sheet Plus 2 worth three $ 599. Hot Roll three ( 01) Uncoated heavy gauge, hole fully processed in Strip Steel never cold reduced at Tandem Mill. Multiple three lifting points allow handling of a variety of pipe lengths.
Taken from past issues sheet of our Magazine. hole Gideon Bible Lessons crafts, activities for Sunday School. Spark Plug Hole Re- Threading. 5/ 160 but hindsight is lifter always twenty- hole twenty. 00* PE Design Plus 2 Embroidery Software gives three you the power to perform basic digitising and transform photos into embroidered works of art through the lifter PhotoStitch™ feature. com is trade of Plastic Machinery platform for sheet injection molding machine blow molding machine, extrusion machine, Rubber Machinery, Plastic Printing Machine, Plastic molds in the Taiwan , Plastic Welding Machine China.
PE770 Sewing Machine pdf manual download. How to use Directions for use. Pushrod Geometry Technique # 1: This lifter technique assumes hole that you have purchased one lifter of our Hi- Tech pushrod length checkers. They carry a large selection of quality violins construction woods, finishes, , technical books, violin parts, strings, accessories, bows hole much more. Atlantic Violin Supplies located three in New Brunswick, Canada, is a violin supply business providing value three service since 1993. This number represents the gauge length of the part (. On second hole thought I should have gone for 352. Log Lifter Plans Now Free.
Search For Log Lifter Plans Now Free. Title: Microsoft Word - Verifying Pushrod Length And Rocker Arm Geometry Author: ssosa Created Date: 7/ 27/ 4: 36: 06 PM. Compare with bulkforming. The plastic deformation of a lifter piece of sheet metal by tensile loads into a three- dimensional shape often without significant changes in sheet thickness surface characteristics. The most notable difference was the ease of which I completed the lift in competition the second time around. 4L Ford Rebuild Cheat Sheet: Before You Begin your DIY project.
Also for: Dz820e Sb7900e, Nv750e, sheet Nv770e lifter Pe780d. 140" gauge diameter) with the. Motorized Sheet Lifters provide the utmost efficiency and ease of use. These are marked with a standard length stamped in them. View and Download Brother PE770 service manual online.
Add Yates Dynamic Lifter Soil Improver and sheet Plant Fertiliser to Wish List ». Sheet lifter three hole. lifter The Best Log Lifter Plans Now Free Free Download PDF And Video. 7 gained on bench was due to a no- lift attempt at 347/ 157. Always water well after application.
Don' sheet t miss to sheet check this sheet to know about Modular V- 8.
Sheet Lifters - 3 Ring - Black - 8- 1/ 2" x 11" ( full page lifter), 2/ pk Item# : AA00110 Keep your Photo Album from buckling or folding with these Poly Photolifters. Heavy duty sheet lifters from Grainger offer versatile handling of bundles, sheets, plates and other materials stacked horizontally. A low headroom design provides optimum lifting capabilities while one person operation affords cost control. IA II Engine Blocks " We' ve built more aftermarket blocks than anyone in the world" Cast of high nickel content cast iron, this block features the following improvements over the original design:.
An excellent alternative to head filling is the use of special domed racing pistons. In the late 1940' s Frank McGurk of McGurk Engineering in Gardena, Calif. Caldwell C- Hook Pipe Lifters.
C- hook pipe lifters handle up to 8' L sections of precast concrete pipe. Pipe lifters eliminate the need to dig a hole under the pipe, increasing productivity.


As its so close to the end of the month I thought I would try to get a few challenges in while I still have time!
As you may have noticed I have been busy completing a belated baby album for my middle daughter (who is now ten) Unfortunately when our computer broke we lost most photos taken from her baby and toddler years which is so sad - I only have two baby photos of my son also!
This month I have been eyeing off the Berry71Bleu challenge - to use die cuts in your layout.
The prize this months is a stunning paper collection with extra dies from Heartfelt creations. I have been eyeing off some of their products for quite some time, especially their die and stamp sets!
I decided to use a double page sketch from this months Page maps after seeing it used for a couple of other layouts. Of course I only had three photos - but it was perfect just the same!
Wonderful 2 page layout--those pictures seem very sweet. Lovely work & great take on the challenge....thanks for joining us at Berry71Bleu!
Beautiful, sweet two pager! Thank you for playing along with us at Berry71Bleu!
Adorable 2-pager! Thanks for joining us at Berry71Bleu!


text:
Q: How do I find which of 2 values are closer to a given number in python? I have 2 variables, value1 and value2. I want to see which of these values are closer to the number 21 through an if statement. So in pseudocode,
If value1 is closer to 21:
    Event1
elif value2 is closer to 21:
    Event2
else:
    Event3

A: def foo(number,val1,val2):
   if abs(number-val1) < abs(number-val2):
      event1()
   elif abs(number-val1)>abs(number-val2):
      event2()
   else:
      event3()

A: You can use the absolute value function and see which difference is greater between 21 and the values:
value1 = 40
value2 = 8

if abs(21 - value1) < abs(21 - value2):
  print('value1 is closer')
else:
  print('value2 is closer')

Output:
value2 is closer

Think about how you would do it normally. You would find the POSITIVE difference between the two values, and see which is smaller!

A: I made this example to check which number is closer:
#Setting variables
val1 = 2
val2 = 1
cond = 21

check1 = val1
check2 = val2

#arrays to save numbers between
arr1 = []
arr2 = []

#Conditioning values

#Value 1 check

if(check1 > cond):
    while check1 > cond:
        # print(check1)
        arr1.append(check1)
        check1 -= 1
elif(check1 == cond):
    arr1 = [cond]

else:
    while check1 < cond:
        # print(check1)
        arr1.append(check1)
        check1 += 1

#Value 2 check

if(check2 > cond):
    while check2 > cond:
        # print(check2)
        arr2.append(check2)
        check2 -= 1
elif(check2 == cond):
    arr2 = [cond]

else:
    while check2 < cond:
        # print(check2)
        arr2.append(check2)
        check2 += 1

result1 = len(arr1)
result2 = len(arr2)
# Checking which value is closer
if(result1 == result2):
    print('Both numbers are equaly closer')

elif(result1 < result2):
    print(f'The number {val1} is closer to {cond}')

else:
    print(f'The number {val2} is closer to {cond}')

redpajama_set_name: RedPajamaStackExchange


Nanaimo: TimberWest is reminding the public it has closed gate access across its operating land base. The extreme fire risk on Vancouver Island has already resulted in wildfires in Port Alberni, Duncan, Bowser, Sooke and most notably the 180-hectare wildfire in Nanaimo affecting private residences, TimberWest land and campsites.
Causes of the wildfires are under investigation with the BC Wildfire Service, however a majority of the fires are suspected to be person caused.
The Nanaimo Lakes wildfire broke out on Sunday, August 5th. The BC Wildfire Service Coastal Fire Centre responded quickly to the scene and TimberWest is actively lending support. On Monday evening a tragic medical incident occurred at Nanaimo Lakes. Sadly, an employee of a contractor passed away while working under the supervision of the BC Wildfire Service Coastal Fire Centre.
The Regional District of Nanaimo has issued evacuation protocols for several residents living around Nanaimo Lakes. TimberWest has closed all Nanaimo Lakes campsites until the fire threat has abated.
TimberWest issued fire bans across its campsites in late June and closed access to its North and South Island private lands in July. Please report acts of misuse or illegal activity to the RCMP Conservation Officers by calling the report a poacher or polluter hotline 1-877-952-7277, and if there is a fire call the BC Wildfire Service 1-800-663-5555, or *5555 on cell phones.
Campsites Open: Gooseneck, Willington Point, Wokas Lake; and Echo Lake and Beavertail day-use recreation sites.
Road Closures: Comox Lake Main (CL 6 to CL31); Boston Main; Browns Main; Cougar Main; Constitution Main; Murex Main; Duncan Bay Main (DBM 31 to DBM 51); Cranberry Main; McBain Main; Airport West Main; JD Main; Elk Main (Quinsam River to Echo Lake);Tom Brown North/Dump Main; Elk 3000; Bacon Lake Main; Berry Creek Main; Quinsam Main; Elk Mtn.; and Buttle Bluffs.
Access for Members of Clubs with Access Agreements: Comox District Mountaineering Club members will have access during sanctioned weekend events organized by the club. Mountain biking will be permitted on trails managed by the United Riders of Cumberland.
Comox Lake Cabin Owners: Road access will be available for cabin owners during this closure period.
Road Closures: All gates are closed to the public from Sooke to Qualicum and Port Alberni.
Road Delays: Road improvements are underway on the North Shore road from Cottonwood Creek to Wardroper Creek. We ask that the public use the South Shore road.


text:
Q: Parse string "20151112 221433" (date) to date-type string "2015-11-12 22:14:33" VB.NET I need to convert an oddly-formatted date string : 20151112 221433
to the following format in visual basic: 2015-11-12 22:14:33
I don't care if it's a DateTime, but I'd prefer to leave it as a string.

A: I would normally convert it first to date like this:
Dim sDate As String = "20151112 221433"

Dim _date As DateTime = DateTime.ParseExact(sDate, "yyyyMMdd HHmmss", System.Globalization.CultureInfo.InvariantCulture)

_date.ToString("yyyy-MM-dd HH:mm:ss")

A: If the string format is for sure the same.. including two digit month/day/second/etc.
than this should work:
Dim s As String = "20151112 221433"
s = s.Substring(0, 4) + "-" + s.Substring(5, 2) + "-" + s.Substring(6, 3) + s.Substring(9, 2) + ":" + s.Substring(11, 2) + ":" + s.Substring(13, 2)


A rather super opportunity presented itself today with GWR green set LA15 working 1C72 to Penzance - in full sunlight to obtain a nice portrait of recently refurbished Par Signalbox (circa 1890) with the HST (circa 1976).
All looking dazzling for the next few years.
Also taken was 57605 almost lost to vegetation on Coombe viaduct with 5P70.
These pictures arrived late last night, as you will have seen the bright sun and two interesting movements brought rather a deluge of pictures.
Hi Keith, As you know, 70806 visited Cornwall today to pick up the railvac from Truro Yard back to Westbury and also the NMT (43062 + 43014) came down as well with the 5:25 Old Oak Common to Paignton via Penzance. 43014 + 43062 pass Truro with the NMT 5:25 Old Oak Common to Paignton via Penzance Thank you Jamie.
The Network Rail Test Train made a striking appearance at Truro 26th August 2016.
This picture taken in 1961 caused us some debate, Steve originally submitted it as being on the Lyme Regis branch but after further debate decided that it was probably Colyford level crossing on the Seaton branch. I think we all agree with this. The platform would have been on the other side of the train. (Mike Roach advises that the first car waiting is a Morris 8; his first car) Copyright Steve Richards N.B. Happily this is still a rail crossing being used by tramcars - part of the Seaton Tramway.
Just a reminder that the CRS will be visiting the Lynton and Barnstable Gala on September 24th - see details in our outdoor events section.
S_SR_760_Blackmoor_A001 The Lynton & Barnstaple always creates a stir as it did with me when I acquired this original negative. The locomotive is one of the original three bought by the L & B and is Manning Wardle built 2-6-2T EXE after it had been renumbered as 760 by the Southern. The location is Blackmoor.
An interesting note on the side: my immediate neighbour was a weekly passenger on the L&BR when he used to accompany his mother to the Barnstaple market.


BlackBerry sounded some static notes on Friday, reporting a big drop in third quarter revenues as its hardware sales flagged.
But the long-suffering smartphone maker reported a surprise bump in operating profits, providing some hope that its turnaround efforts are starting to gain traction.
BlackBerry's revenues fell 34% to $793 million from $1.19 billion. That's far below the $931 million in revenues analysts were expecting.
But the company shrunk its net loss to $148 million, or 28 cents a share, vs. a year-ago loss of $4.4 billion, or $8.37 a share. On an operating basis, BlackBerry broke into the black, generating $43 million in cash flow.
The earnings report, which came two days after BlackBerry released its latest handset, dubbed the Classic, sent shares falling 0.8% Friday, to close at $9.99.
Under new CEO John Chen, who joined the company more than a year ago, BlackBerry has cut costs, launched new devices and grown its security software business.
During a conference call on Friday, Chen called the third quarter revenue numbers "not satisfying" and said he will need another couple of quarters before revenues will turn the corner.
The Classic has a physical keyboard — like the BlackBerry of old — a larger screen and an expanded app library with access to Amazon's Android App store.
Colin Gillis, an analyst at BGC Partners, one of the few Wall Street analysts with a buy rating on the stock, saw positive signs in the third quarter report.
"Current management is executing its turnaround plan," Gillis told the Daily News. "A big milestone is when you stop burning cash and turn cash positive. It means you can pay your bills."
Licenses for BlackBerry's software have grown to 6.8 million in the quarter from 3.4 million in the previous quarter, Gillis added.


text:
Activity Tracker Reviews
Bluetooth Transmitter
ATechReview
Home Drones DJI Mavic Pro Review
DJI Mavic Pro Review
Most Advanced & Agile Quad Available
Marko Jovanovic
The DJI Mavic Pro is a flying, remotely operated drone – a small hovering device that can be used for multiple purposes. Basically, it is a remotely operated camera with attractive design. We're going to review the features, the advantages, and the uses of DJI Mavic Pro further in this review.
How does DJI Mavic Pro Work?
This drone operates on batteries which are remotely controlled by a special controller. It is also supplied by wireless connectivity feature, which is a new addition to its smart feature. In short, you will use your remote controller to navigate the drone the way you want it to go.
The Features and specifics of DJI Mavic Pro
There are many features that the DJI Mavic Pro comes supplied with, but most important ones are the Aircraft features, Remote Controller Features, the Camera Features, the Wi-Fi features, and The Flight Battery features.
The Aircraft Features are separated into several sub-features, such as the folded size, the diagonal size, the weight, the maximal ascent speed, the maximal descent speed, the maximal overall speed, the maximal flight time, and such.
Size: DJI Mavic pro is 83mm by 83mm by 98mm in size when folded, it's 335mm in diagonal size. It's 1.64 pounds heavy with gimbal cover, it can reach a maximum of 16.4-feet per sec of ascent when it's in sports mode, and can reach a maximum of 9.8-feet per second of descent speed.
The maximum speed it can reach is 40-miles per hour, and this is measured in the perfect weather conditions (no wind). It can fly for 27 minutes. If we take into consideration the 15% of low-battery warning, it can fly for 21 minutes overall.
The Remote Controller
The DJI Mavic Pro comes supplied with a special remote control that possesses its own unique features. It operates on the frequency in the range between 2.4 GHz and 2.483 GHz, and it has a maximum of transmission distance of 4.3 miles (FCC compliant), or 2.5 miles when unobstructed.
The battery that the remote controller needs are (ideally) a 2970mAh, but it can work on similar batteries as well. Keep in mind that using a substitute for the recommended batteries might affect the overall performance.
The camera is one of the most important features of the DJI Mavic Pro, so it is only natural that it's a high-quality one. These features can be split into further sub-features such as the Sensor module, ISO range, Shutter Speed, Image Size, Still Photography settings, Video Recording Settings, Maximal Bitrate for Video capturing, Supported formats, Photo formats, Video Formats, SD cards, and the Operating temperature.
The Sensor module features a CMOS sensor of 1" by 2.3", and has a total of 12.71 megapixels. A total of effective megapixels is 12.35. The focus lens has a diameter of 28mm, and the predicted distortion is below 1.5% (when maximally focused).
ISO range is between 100 and 3200 for videos, and between 100 and 1600 for photos. The Image Size is really important, as you wouldn't find much use of a drone that could take panoramic shots in small sizes. DJI Mavic pro can take photos of up to 4000×3000 resolution.
Photography settings are the options of how you would like your photos to be when you take them. You can take a single shot, burst shots at intervals between 3 to 7 frames, AEB shots in the bracketed frames of 0.7 secs, EV Bias shots, or Interval Shots.
Video Recording Settings are the resolutions of the videos your drone will take. These modes are C4K – 4096 by 2160 in 24p; 4K – 3840 by 2160 at 30p; 2.7K – 2704 by 1520 at 30p; FHD – 1920 by 1080 at 24-96p; HD – 1280 by 720 at 24-120p. Maximal Bitrate for Video capturing is 60 Mbps.
Supported formats are FAT32 for files that are equal or below 32 GB, or EXFAT for files that are beyond 32 GB. Photo formats that are available for DJI Mavic pro are JPEG and DNG, and the Video Formats are MP4 and MOV. The operating temperature of the camera is between 0 and 40 degrees Celsius.
The Wi-Fi
Even though DJI Mavic Pro operates on batteries (which are operated by the remote controller), it is supplied with a wireless connection feature that further increases its capabilities. Considering the Wi-Fi settings of this drone, the operating frequency is between 2.4G and 5G, the Maximal transmission distance is 80m in distance and 50m in height (when unobstructed)
The Flight Battery
The special battery that DJI Mavic Pro operates on is very important as it keeps it going. It is very important to remember that the performance of the drone will get significantly lower when the battery reaches 15%. This is the time at which you will need to recover your drone and recharge it.
When it comes to specifics, the Flight battery capacity is 3830 mAh, the voltage is 11.4V, it uses a special type of battery that is called Lipo 3S (Here is a guide to understanding Lipo 3S Battery), and it has the energy of 43.6 Wh. Similar batteries can be inserted only as a substitute – you should not rely on similar battery models as the overall performance of DJI Mavic Pro might decrease.
What are the possible uses for DJI Mavic Pro?
There are virtually limitless uses for this little drone, as it is very practical, lightweight, it has a strong battery lifetime, and has many special features. You can use it as a toy/for entertainment (even though it costs quite a lot to be considered a toy), for doing beautiful aerial photography, taking aerial videos, as a scouting recon while you're in a territory/wilderness that is unfamiliar to you, and more.
If you use it for entertainment, you will surely get hooked pretty easily. It is very fast and it can hold out for around 30minutes (which is better than industry standard now), but the only reason why this purpose is advocated against is because it costs quite a lot. If you're searching for a drone for entertainment, there are cheaper models that can provide you with it.
Why is this made for? If you are using DJI Mavic Pro for pictures/videos/panoramas, you are using it for the purpose it was made for. It has a very powerful camera that can take very large and sharp pictures and 4k videos with Stabilized Camera camera with 3-axis gimbal stabilization.
Lastly, if you're using it for recon in the region you are unfamiliar with, you will be very satisfied with the results. It has an enormous range and the obstacle avoidance feature which will ensure that no harm is done to it.
The advantages of using the DJI Mavic Pro
There are a lot of drone models on the market, and it is only natural to ask "why is DJI Mavic Pro better than the others?". The answer would not be so simple, as there are several fields at which it excels beyond the standards of a regular drone model.
It is faster and safer than most drones
Having the Maximal Ascent Speed of 16.4-feet per second means that DJI Mavic Pro can travel as quickly as a Hummingbird. Simply being fast doesn't mean a lot if you end up crashing your drone. They are very expensive, so you would either need to be extra careful or refrain from using it a lot.
However, this is not the case with DJI Mavic Pro. It has a special Obstacle Avoidance feature that triggers safety sensors whenever an object is in its pathing, so it will override the controls you are giving him if it means a certain collision.
It is equipped with an extremely powerful camera
In the terms of Video and Picture quality, DJI Mavic Pro stands on the top. The 12-megapixel camera can take photos in Adobe DNG RAW format and take 4K HD videos in 30fps represent the pinnacle of quality. Most drone models offer either the video quality or the picture quality, while this little beauty has it all.
It is lightweight and compact
When you fold this drone, it is extremely small (83mm by 83mm by 98mm), making it extremely compact and easy to carry around. This feature is improved with the lightweight design – DJI Mavic Pro weighs only 1.64 with gimbal cover.
Overall Pros and Cons of DJI Mavic Pro
DJI Mavic Pro is not perfect. It possesses a lot of extraordinary features that make it very valuable, but it has some flaws as well. While we already mentioned most of the virtues it has, we will summarize them along with the flaws.
Extremely capable of taking pictures and videos of high quality;
Very fast ascent and descent speed;
Great range;
Obstacle Avoidance feature;
Lightweight and compact design;
Specialized batteries;
Mediocre flight time;
Steep price;
The flaws of DJI Mavic Pro can easily be overlooked if we take into consideration the advantages it offers. Even though it's not perfect, it is very durable and it's built to last. All in all, DJI Mavic Pro is compact, lightweight, very fast and reliable, has a great range, it's capable of taking pictures and videos of the highest quality, but it comes at a very high price.
If you are a professional videographer or photographer who loves to take outstanding aerial photography or even landscapes, or if you are a Dron enthusiastic, or if you are a Dron racer, this is your gear. If you don't take Dron or aerial photography seriously, this is an expensive Dron for you.
SOURCEDJI
Previous articleBLU Advance 5.0 HD Smartphone Review
Next articleMoto G Play 4th Generation Review
Black Friday Drone Deals Predictions
Best Drone Backpack – Reviews and Comparison
Case Club DJI Phantom 4 Case Review
Smart Recommendation
Best 17 Inch Laptops In 2019: The 10 Best Wide Screen...
Bluetooth Bookshelf Speakers – A comprehensive Buying Guide
13 Best Wireless Surround Sound System in 2019
ATechReview.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to (amazon.com, or endless.com, MYHABIT.com, SmallParts.com, or AmazonWireless.com).
Dell Inspiron 15 5000 (2018) Review
Headphones13
Black Friday 2018 Deals13
Drones10
Phones6
ATechReview.com is your tech news, review, how to tutorials and shop. We provide you with the latest breaking news, product reviews from tech industry.
© All rights are reserved by ATechReview.com


In more than 15 years in the financial services industry I worked with many different investment theories and with almost as many different kinds of investors. Some were extremely conservative and would only invest in bonds or CDs while others were aggressive speculators dabbling in various options strategies or betting on the futures market. There were the technical investors with their charts and graphs looking for trend lines and breakout patterns and those who followed the fundamentals such as the earnings and growth prospects of various companies. What stood out for me, however, were the stock market investors who favored growth or value. A growth investor would seek out companies that had the potential for future earnings growth, and therefore, price appreciation. The value investor would search for companies that were undervalued by the market with the idea that at some point the market would recognize the discrepancy and the stock price would rise.
While both investment theories were valid, market conditions had a tendency to favor one over the other at different times. Within the industry we were constantly on the lookout for a change in the current trend. Were we in a growth phase or was the trend turning towards value? An investor who spotted the change in trend at an early point could shift strategy and benefit from the changing climate. However there were many who could not spot the change until well after it occurred and were always chasing the market after it had shifted. A bull market run up in the stock market didn't end gradually, it usually ended with a speculative blow-off. Inexperienced investors would jump in and the end of the bull market cycle and bid prices up to unreasonable levels. The rationale was that "it was different this time", "it's the new economy", "old rules don't apply anymore", and hearing statements like this was usually a signal that the pendulum was about to swing back in the other direction. Most bull markets would end with a correction where prices would fall a bit and start moving up after a period of time. A much steeper drop, such as the 1987 stock market crash, usually followed a major market run-up. Novice investors would run from the market with their tail between their legs vowing that they would never buy a stock again. The value investor loves a market decline. This is where he could step in a buy solid stocks that had been oversold and suffered a price drop that was below the theoretical value of the company. These bargains could be scooped up and held on to until the next rise in market prices.
In comparing the real estate market to the stock market you have some very significant differences. The stock market has almost instant liquidity, you don't need to advertise your stock or list it on the MLS and wait for a buyer. A stock doesn't have carrying costs, mortgage payments, vacancies etc. What the real estate market does have is the extraordinary power of leverage. Through the use of a margin purchase you can obtain a maximum 2 to 1 leverage on a stock but in real estate a 5% down payment gives you 20 to 1 leverage. This means that if you put 5% down and your property appreciates only 5% you have doubled your money!
The parallel that we can draw from the stock market is that the bull market in real estate has peaked and we definitely saw it end with a speculative blow-off. The wanna-be investor cashed in his home equity and used easy mortgage money to buy investment property because, after all, everyone knows that real estate always goes up. This was what Alan Greenspan once called "irrational exuberance" or a frenzy of speculation. Prices were bid up to unsustainable levels and there had to be a correction in the market. Where are all of the investors and agents who claimed that it was not a problem to have negative cash flow because you were going to make so much more in appreciation? Perhaps they realize now that what they were touting wasn't investing but speculating. These inexperienced investors are being forced out of the market, many of them never to return.
The value investors are now able to come into the market and find properties at bargain prices. Cash flow is king once again and built-in equity is paramount. The current glut of unsold homes will eventually be sold and the market will return to a level of sanity we haven't seen in a while. The get-rich-quick schemer will find a new fad to throw his money at. The savvy real estate investor will buy properties because they make economic sense, not because he is speculating on price appreciation.
What we can learn here is that while every type of investment has its' own unique characteristics, they all share some similarities. The most notable common trait is the human element. When greed overtakes sanity and reason you will find people making rationalizations to justify their behavior. All of the excuses as to why this market is different are your signal to head for the exits and wait to capitalize on the change in the trend.
I prefer the stock market over real estate. I have been able to get a better return in a shorter amount of time.
Chad, dont you think you could lose it in a shorter amount of time also.
"Perhaps they realize now that what they were touting wasn't investing but speculating" I agree to this statement about inexperienced investors but I think new and young investors should learn from the mistake. Learn to invest not to speculate and speculating is tune to gambling – Use the brain not the heart…..
I love the stock market. I think if practiced properly, you can make money in all market. Also, theres less paperwork involved.
Real Estate is always a better investment than the stock market. You have something tangible and it increases in value over time. Stocks are too unpredictable unless they are stocks of brick and mortar companies. The only companies that I would really suggest are railroads because they are going to carry freight for a long time to come and the rate of returns are good.
Looking back at these comments and you can see how the housing bubble got started.
Homes may be tangible, but they have a value to them as well. Everything has a value. When you start thinking that house prices only go up, that's the pitfall. Sure house prices go up over many years, slowly, but so has the stock market.
Depending on what field of expertise you are in is a better factor. I don't know much about real estate so I can get much better returns from the stock market. Vice versa.
"Cash flow is king once again and built-in equity is paramount." This is so true. Look at F, a company that in the last year and a half has had strong earnings, but recently has been plagued by short sellers. Not a bad entry point IMO. Keep up the good work guys.


Attempt to store backup from a network drive.
Ensure the device is not hosting multi user access.
Restore the backup stored saved on your system.
Check if the error still occurs.
In case the error still persists, try altering your computer file name such that the character limit doesn't exceed 120 characters.
The backup is hosted by quickbooks online backup or on a network drive, saving a copy to your local computer should be sufficient.
In the search box, type "command".
Click and hold CTRL+SHIFT and hit Enter on your computer.
Click yes, on the prompted permission dialog box.
A black box will appear with blinking cursor.
Select the error 6147 related key in the registry editor, you want to back up.
Choose Export, from the file menu.
Select the folder where you want to save the Quickbooks backup key from the save in list.
Type the name of your back up file, such as "Quickbooks backup" in the file name box.
You may now have a backup of your Quickbooks related registry entry.
Over time the device accumulates junk files from computer use and web surfing. In case the junk is not occasionally cleaned out, it may cause Quickbooks to provide an error 6147 or respond slowly, possibly due to file conflicts or an overloaded hard drive.
Click and hold CTRL+SHIFT on your keyboard and hit Enter.
Hit Enter after typing "cleanmgr".
Disk clean up will begin.
Check the categories in the dialog box you want to clean and click OK.


text:
Port paves way for 'new era of economic affluence' in Saudi Arabia
Port paves way for 'new era of economic affluence' in Saudi Arabia /node/1450576/saudi-arabia
Crown Prince Mohammed bin Salman (center) at the launch of the King Abdullah Port on Monday. (SPA)
Ruba Obaid
'A link between East and West,' Jeddah mayor says at launch
The port is aimed to make Saudi Arabia a global logistics hub
JEDDAH: Saudi Arabia moved one step closer to its goal of becoming a global logistics hub with Crown Prince Mohammed bin Salman's launch of the King Abdullah Port in Rabigh on Monday.
The port, part of King Abdullah Economic City, will make the Kingdom "a linking point of three continents, Asia, Europe and Africa," the crown prince said at the launch.
King Abdullah Port, the fastest-growing container port in the world, is considered a major success story in the Kingdom's efforts to develop public-private sector partnerships.
The port's aim of positioning Saudi Arabia as a global logistics hub is in line with the Kingdom's Vision 2030 economic reforms.
"When we talk about King Abdullah Port, we are talking about Vision 2030," Rayan Mustafa Qutub, the port's CEO, told Arab News.
As part of Vision 2030, Saudi Arabia is investing heavily in the construction of ports, railways, roads and airports. The government plans to work with private- sector investors and enter into a new series of international partnerships to link the infrastructure developments internally and across borders.
"One of the biggest sources of the economy in the Kingdom's vision is logistics, which is forecast to become 33 percent of the Kingdom's economy by 2030," Qutub said.
The port will act as a link between Gulf countries through improved logistics services and new cross-border infrastructure projects.
بث مباشر | سمو ولي العهد يدشن #ميناء_الملك_عبدالله في رابغ https://t.co/aa8sbE3yXb
— وزارة الإعلام (@media_ksa) February 11, 2019
Qutub said King Abdullah Port would establish the Kingdom as a platform for the region.
"The countries of East Africa are the new China, the next huge neighboring economy, and we can now exchange exports and imports, and offer logistic services to them and the world as a whole," he said.
King Abdullah Port, about 120 km north of Jeddah, features the world's deepest 18-meter water berths and state-of-the-art processing facilities.
The port's container terminal's current capacity of 3 million TEU — or 20ft equivalent units, a measure of cargo capacity — will increase to 5 million TEU by the end of the year, according to Qutub.
"King Abdullah Port ended 2018 with an annual throughput increase exceeding 36 percent, a record. If we maintain this growth rate over the coming years, we will achieve our goal of 20 million containers per year very soon."
Saleh Al-Turki, mayor of Jeddah, said that the port "will add huge value to the city of Jeddah."
"This is a huge event. The port makes Jeddah the number one logistics hub in the Middle East. It connects East to West," he said.
The port's expansion will encourage further development in the region, creating jobs, increasing urbanization and delivering economic affluence, Al-Turki said.
Topics: King Abdullah Port Rabigh KAEC Saudi Vision 2030
Container handling up 45% at King Abdullah Port
Rabigh Dam project to supply 500,000 beneficiaries in Jeddah and nearby areas


Martiniano Leguizamon (ML) chat rooms are where you should be right now because you are in search of an interesting chat partner or a spicy online relationship. We group many online people from Martiniano Leguizamon (ML) in a common chat room and allow them talk to each other about different things to talk about. You will surely become addicted to our crowded Martiniano Leguizamon (ML) chat rooms where you will be able to talk about any topic you find interesting, meet like minded men and women and find dating partner. Martiniano Leguizamon (ML) chat rooms are the best anonymous chat services. If you are bored, in need of a friend or simply looking for some decent chat fun, our Martiniano Leguizamon (ML) chat rooms are the best choice for you. Have fun in our Martiniano Leguizamon (ML) chat rooms where hundreds of interesting chatters are looking for an amazing companion like you!
All you have to do it type a nickname and press the enter chat button to participate in the chat. Signup pages, making account and other mess are the boring steps which you never have to go through. Our chat rooms don't cost a penny, moderated & updated where you can anonymously talk to boys and girls from all over Martiniano Leguizamon (ML) and talk about topics you find amusing, get to meet like minded social users, make boyfriend/girlfriend. You might come across someone from your very own place you never knew. At yesichat, you don't have to login or register or validate your email in order to start chatting in our Martiniano Leguizamon (ML) chat rooms. A large number of visitors land in our crowded Martiniano Leguizamon (ML) chat rooms in search of a engaging conversation.
The best thing about our Martiniano Leguizamon (ML) chat rooms is that we dont require you to create an account through email verification. Online Chatting is very relaxing and when you find surprises that you never thought existed, its even more amazing. Who would not be excited to find new friends? Are you interested to find friends from Martiniano Leguizamon (ML)? Have you been searching for an interesting chat room that doesn't require you to sign up or register. Meeting strangers, finding a date and chatting freely about different topics was never this intuitive before we created our Martiniano Leguizamon (ML) chat rooms.
You wont ever be bored next time onwards when you have to be alone because we wont ever let you be lonely once you join our crowded Martiniano Leguizamon (ML) chat rooms where you get to find open minded men and women who are looking for an amazing chat partner like you. We help people like you to spice up their typical lives by allowing them meet like minded online chatters from different states of Martiniano Leguizamon (ML) in our moderated Martiniano Leguizamon (ML) chat rooms. We don't charge for our guest Martiniano Leguizamon (ML) chat rooms where you do not have to sign up or login or go through the boring email verification process. You can talk about interesting topics, meet new peopl or find a boyfriend/girlfriend in our chat rooms. Whether you are just looking for a decent chat conversation or trying to find a partner for relationship, our Martiniano Leguizamon (ML) chat rooms definitely will be helpful.
The unique Martiniano Leguizamon (ML) chat rooms at yesichat do not require you to sign up. Just clicking a button allows you to to participate in the chat and engage in conversation with local users from Martiniano Leguizamon (ML). A lot of strangers from Martiniano Leguizamon (ML) with interested in friendship and talking to strangers visit yesichat. Crowded Martiniano Leguizamon (ML) chat rooms are one among the best destinations to meet like minded people. Our fun and simple to use Martiniano Leguizamon (ML) chat rooms attract a large number of internet users from different cities of Martiniano Leguizamon (ML). All of them are looking for a awesome partner to share some giggles in a decent online conversation. Its not hard to make friends or talk to strangers in our well organised Martiniano Leguizamon (ML) chat rooms as there are hundreds of awesome and open minded people managed together for online chatting.
You do not have to register for chatting online. Simply typing a nickname allows you to use Martiniano Leguizamon (ML) chat rooms without verifying your email.
Martiniano Leguizamon (ML) chat rooms are amazing not only due to the reason that its packed up with like minded fun people but also because they are provided a suitable social platform to express their views, meet new strangers, organise interesting group conversations, make friends and find their dream partner for relationship.
Clicking the 'Start Chatting' button gives you instant access to our free and fun engaging community of Martiniano Leguizamon (ML) anonymous visitors who talk together every day in our Martiniano Leguizamon (ML) chat rooms.
Martiniano Leguizamon (ML) chat rooms are one among the most crowded chat rooms on web.
Who on earth would register for any online chat room service when you can enjoy guest chatting service in our Martiniano Leguizamon (ML) chat rooms that allows you to have fun without signing up. No email verification is needed to start using our awesome Martiniano Leguizamon (ML) chat rooms. All you have to do is to hit the start chatting button. Our Martiniano Leguizamon (ML) chat rooms are all the time packed with a large number of interesting boys and girls of different ages and genders looking for a like minded buddy to have a relaxing and funny conversation. Online chatters find our chat rooms quite interesting and use it in different ways to make friends and talking to strangers.


text:
Q: CSS img height with % not working i'd like to resize an image (and the slider containing the image).
The following css code works fine:
.slick-slider .img-responsive{
  max-width:none;
  min-width:100%;
  width:100%;
  height:300px;
  object-fit: cover;
}

The object is achieved: the image/slider fills the whole width of the screen and takes only 300px of height. object-fit:cover crops the image.
But because i'd like to work responsive i want to change the height to a percentage value.
But if i replace 300px with 30% nothing happens (-> meaning the image has the full height, nothing is cropped).

A: Could you try this for me:
.slick-slider .img-responsive {
   min-width: 100vw;
   width: 100%;
   height: 10vh;
   object-fit: cover;
}

This forces your image to have a height of 10% of the viewport rather then pixels or percentages. It should create a responsive layout, let me know if it works out, and otherwise share a jsfiddle so I can poke around.


Social tennis is a great way to meet your fellow club members. Both Saturday and Sunday cater for players with a wide range of abilities and skills. Games are organised to enable members to play with others of similar standard in a mixed, ladies and men's doubles format. A short break is taken at 3.30pm for complimentary afternoon tea.
All new members and visitors are warmly welcomed.
Junior club is held on Friday afternoons from October to April each year. This is a great family afternoon. Junior members aged 12 and under play social tennis from 4.30-6pm, while children aged 13 and over play from 5-6.30pm.
Social play for juniors includes doubles play and then an organised fun game to finish.
* Note: Grass Courts are closed on Monday morning and all day Thursday for maintenance.
Synthetic and hard courts are available for hire during the day for $10/hr or at night for $15/hr.


text:
U.K. Regulator Clears Broadcaster Over Piers Morgan's Meghan Comments
Mayeiah
High – profile television personality Piers Morgan did not breach Britain's broadcasting code in his criticism of Meghan, Duchess of Sussex on ITVs 'Good Morning Britain' show earlier this year, Britain's media regulator ruled on Wednesday.
The programme in March, in which Morgan said he did not believe a word Meghan had said in a landmark interview she and her husband gave to Oprah Winfrey, attracted more than 50,000 complaints. Most concerned comments Morgan made on mental health and suicide.
Meghan, who during the Winfrey interview revealed she had felt suicidal while living as a royal in Britain, complained to both Ofcom and ITV about the programme. Morgan left his job on the show following the incident.
'Our decision is that overall the programme contained sufficient challenge to provide adequate protection and context to its viewers', Ofcom said.
In an interview that shook Britain's monarchy, Meghan, a Hollywood actor who married Prince Harry in 2018, said the royal family had also rejected her please for mental health support. The couple have stepped back from royal duties and moved to the United States.
'I'm delighted OFCOM has endorsed my right to disbelieve the Duke & Duchess of Sussex's incendiary claims to Oprah Winfrey, many of which have proven to be untrue', Morgan said on Twitter.
'This is a resounding victory for free speech and a resounding defeat for Princess Pinocchios. Do I get my job back?'
A.U.S – based spokesperson for the Sussexes was not immediately available for comment out of working hours.
Ofcome said that while it was concerned about Morgan's apparent disregard for the seriousness of anyone expressing suicidal thought, the extensive challenge offered by his colleagues throughout the show provided sufficient context.
'The restriction of such views would, in our view, be an unwarranted and chilling restriction of freedom of expression both of the broadcaster and the audience', it said.
This entry was posted in ROYAL WORLD, ROYALS and tagged DUCHESS OF SUSSEX, Duke and Duchess of Sussex, PIERS MORGAN. Bookmark the permalink.


Arca's solutions help people control and streamline cash operations in financial institutions, retail stores, and self-service kiosks around the world.
We develop technology and services that make transactions simpler, more efficient and more secure.
Arca has acquired the CTS Group, an Italian provider of cash, check and card automation technologies. The acquisition combines CTS's proven engineering and R&D capabilities with ARCA's strong commitment to the customer. The combined company will provide banking and retail clients around the world with customer-driven solutions based on cutting-edge technology.
Arca is a leading manufacturer of cash and cheque processing solutions for tellers, back offices, self-service systems and retail point-of-sale counters.
Based in Ivrea, Italy, the Arca Group has sold over one million banking peripherals globally and pioneered cash recycling and check imaging technology. The town of Ivrea, located in the Turin province, has a rich history as home to renowned technology companies including Olivetti, which is known for creating the world's first commercially produced personal computer.


text:
Home » Blogs » donna's blog
Small Business in Victoria
Submitted by donna on 28 October, 2011 - 10:10
I've just discovered today is the deadline for making a submission to the Victorian Government on issues facing small business. If you're interested, and can find the time on a busy Friday, you might care to review the discussion paper and make your own submission.
Have your say on the future direction of small business policy and services in Victoria.
There are more than 495,000 active small businesses in Victoria1, contributing approximately 30 per cent of the State's production2.
Through self-employment and employing others, small businesses are a significant source of job creation, providing nearly half (47 per cent) of the state's private sector jobs - around 1.2 million jobs.
Most (62 per cent) active small businesses are start-ups, independent contractors or sole proprietors, and are increasingly home-based.
THE HON LOUISE ASHER MP
Minister for Innovation, Services and Small Business
Responses are due by close of business today - 28 October 2011
Roger Arwas - roger.arwas@dbi.vic.gov.au
Executive Director Small Business Victoria
GPO Box 4509 MELBOURNE VIC 3001
Here is the list of questions asked in the discussion paper. But it also asks for submissions on any other areas of interest or concern pertaining to small business in Victoria.
What are the major taxation, regulatory or compliance burdens facing your business? What are the specific issues that impede your business productivity and growth? What effect will a carbon price have on your business?
Has your business or industry sector been impacted by policy areas that were traditionally regulated by State Governments now being regulated nationally?
As a small business owner, what type(s) of information or advice is hardest to find?
How easy or difficult is it for you to access information about Government grants and other assistance programs?
How can the Government improve access to information and advice for small businesses?
Have you used the services of business.vic.gov.au, the Victorian Business Centres or the Small Business Information Centre/Shopfront? Are these services useful for your business?
Have you used any of Small Business Victoria's programs and services? How could these programs and services be improved? What types of small business support services would you find most useful?
What are the major barriers to accessing business finance and have you found it harder to manage cash flow since the Global Financial Crisis? Are you aware of the Business Loan Finder and is it a useful tool?
What workforce skills are hardest to find or retain? What are the main barriers to finding and keeping the right staff?
What are the significant workplace relations/OHS issues confronting your business? Have these issues risen or fallen in importance in recent years?
Are you finding that your workforce is getting older? What opportunities or challenges does this present?
As a business owner, are you considering sale or succession of your business? If so, what information or advice do you need?
What information or advice would be most valuable for small businesses to help them prepare for and manage risks associated with a natural disaster? What can Government do to assist?
What are the major barriers for small businesses engaging with large purchasers – including with Government?
Is alternative dispute resolution of the type provided by the Victorian Small Business Commissioner the most effective way for small businesses to resolve their business to business disputes and minimise business disruption?
Does your business use the internet extensively? If so, what for? If not, what are the major barriers (e.g. cost, skills, access to reliable services etc.)?
What support or information could the Victorian Government provide for your business to help improve its export readiness and competitiveness?
What are the major challenges faced by small business franchisors and franchisees? How can Government assist?
Do you know of any Government services for small business offered elsewhere that could be considered for Victoria?
What Government support would be most valuable to help Victorian small businesses address current challenges and what should be the principal role of a State small business department?
small business victoria discussion paper 2011.pdf 546 KB


KUALA LUMPUR: The Exchange 106 is ready to enter the scene as Kuala Lumpur's latest architectural gem. It will become a central and functional part of the Tun Razak Exchange (TRX), a massive 70-acre development in the heart of Kuala Lumpur designed to become Malaysia's international financial hub.
This mega structure will also transform the way the biggest and most complex infrastructure projects of the future are delivered. The development is undertaken by Mulia Property Development Sdn Bhd, a subsidiary of the renowned Mulia Group of Indonesia.
China State Construction Engineering Corporation is the lead contractor overseeing this evolutionary infrastructure using new technologies and modern practices with a multi-national, highly-skilled professional workforce.
The company assures complete transparency and sharing of technologies and technical skills with their Malaysian counterparts in particular. High-rise construction expert Doka was selected as formwork technology partner based on its years of experience and use of innovative approaches.
New technologies used for this project include the tower crane and concrete pump which have the advantage of operational efficiency, with the potential to save time, labour and money, besides contributing to faster completion of the project.
In April 2017, the pouring of concrete for Exchange 106 on an area the size of eight Olympic size swimming pools was completed in about 48 hours, making it one of the largest concrete pours recorded globally.
"We actually designed the building specifically in a way that we could erect it and put it together very quickly. We developed a very specific strategy to fast-track the project and it went exactly the way we planned," said Mulia's Project Director Roland Suckling.
Another remarkable feature of the Exchange 106 structure is that, while work has been ongoing at the central core of the tower, the glass cladding too is being fixed simultaneously.
The core of the structure is made of reinforced concrete, and the floor slabs are of steel-composite design. Working closely with high-rise specialists, Doka developed a practical formwork and safety concept adapted to the tight construction schedule and the customer's high safety requirements.
This luxurious skyscraper is being built with some of the most advanced and fast-track construction strategies while adhering to very high standards of safety and security.
Around 2,000 workers of various nationalities have been involved in the construction, working on 22-hour shifts every day of the week, enabling construction speed of one floor in just three days.
Upon completion, The Exchange 106 will be taller than the landmark Petronas Twin Towers. Topped with a 48 meter, 12-storeyed high illuminated crown at its peak, the tower will stand at 492.37 metres.
Designed to the highest specifications, The Exchange 106 is also environmentally sustainable, certified under the Green Building Index (GBI) and Leadership in Energy and Environmental Design (LEED).
With MSC status, The Exchange 106 offers some of the latest in-building technology including high performance insulated glass, energy efficient MEP systems with Star rating, 100 percent LED lighting and state-of-the-art lift technologies.
The Exchange 106 will also feature high ceilings finished in English Burl wood, marble floors and walls of the highest quality in the lobbies and common areas, and large column-free office floor plates of up to 34,000 sq ft with long spans, designed for flexible layouts and open plan interior configurations.
A grand opening is scheduled for the third quarter of 2018 after which The Exchange 106 will take its place as another architectural landmark in Kuala Lumpur's construction history.


NBC and Travel Nevada have teamed up to offer unbelievable great getaway packages.
Just in time for a quick summer getaway, Nevada is offering limited exclusive travel packages, discounts and special deals only for NBC viewers.
Planning a vacation should be easy, so Nevada created themed packages for the ultimate Nevada getaway. Whether you're longing for a little relaxation or action-packed full blown adventure Nevada has it all.


text:
WMC Recap: Dim Mak Pool Party + Club Space with Bassjackers and Ferry Corsten
March 22, 2013 Kathy Vitkus Leave a Comment
Thursday it was all about the Dim Mak Pool Party with Steve Aoki and friends at the Raleigh Hotel Pool. His "friends" included DJ Carnage who greeted with a dubstep version of "Save The World." Dimitri Vegas & Like Mike offered up a mix rendition of "Sweet Child O' Mine" into Daft Punk's "One More Time." Sidney Sampson delivered spot-on blends of electro house, progress house and dubstep.
Dim Mak's head honcho Aoki set it off with his Afrojack collaboration "No Beef." It made sense because the surprise guest Afrojack was actually in the booth manning the decks at the moment. Aoki dropped another crowd pleaser with Kid Cudi's "Pursuit Of Happiness."
The sets were slathered with water blasts, cryogenics, champagne sprays, crowd rafting and cake — lots and lots of cake. Aoki aggressively burrowed into an oversized sheet cake, mixing it up before offering teaser samplings to front-row fans. With the crowd roaring anticipation to be one of his welcome victims, Aoki heaved the massive cake to the first few rows of willing fans to more uproarious applause and encouraging. All the while he played Borgore "Decisions" or commonly and affectionately referred to as the "Bitches Love Cake." He spun for maybe 30 minutes then rode in a raft with a buddy. He sprayed the crowd with champagne and went running around for another half hour while the crowd ate it all up (literally). The tracks magically transitioned and one had to wonder how this is possible and who's manning the decks. Sometimes it was electro house veteran Benny Benassi, sometimes Afrojack and occasionally Aoki. It was a spontaneous team effort. Aoki introduced a new dub-inspired track he said he wrote with Flux Pavillion so that's one to watch for.
Electro-house DJ/producer Felix Cartel took to the decks in a massive way dishing out energetic and thumping tracks right from the start. He played his own, winning "No Sleep" and wound down with one of many remixes of "Clarity." He wasted no time instigating the crowd and keeping them engaged even after his follow-up to his Dim Mak boss's antics. Mr. Cartel also should be another on everyone's one-to-continue-to-watch list.
The night closed out (or was just getting started) with a cab ride over to Miami's Club Space for an opening, heaping, lively, earful from Bassjackers. Each and every track was just as kicking as the previous. Note-to-self, look into Bassjackers mixes or podcasts. He dropped a remix of the fun-loving, vocoder-laden "Easy" from dynamo's Porter Robinson & Mat Zo. When he dropped a remix of "Maximal Crazy" it seemed a subliminal reference to the substantial crowd, ambiguous winding lines and hysteria to get into the venue tonight.
Ferry Corsten followed up with one of his winning archives "Rock Your Body Rock," last year's captivating "Check It Out" and his melodic "Live Forever." Menno de Jong and Giuseppe Ottavianni were also on hand mingling with the A-list VIP's.
Tritonal was in house rounding out the lineup and celebrating posting the following on Facebook, "Let's all wish my best friend and awesome partner, David Reed, a HAPPY BIRTHDAY!! Let's rock this party dude!!" A perfect example of DJ's partying in true style.
Images by Kathy Vitkus
Featured Post, Features	Afrojack, Bassjackers, David Reed, Dim Mak, Dimitri Vegas & Like Mike, DJ Carnage, Felix Cartel, Ferry Corsten, Giuseppe Ottavianni, Mat Zo, Menno de Jong, Porter Robinson, Porter Robinson & Mat Zo
Afrojack Signs Global Deal with Universal Music Group
March 14, 2013 Yehouda Silverman Leave a Comment
If you were wondering why Afrojack went out and spent $240,000 (and promptly crashed an hour later) a Ferrari 458 Italia last month, well, it might well be because today Universal Music Group announced it had signed him to a global deal and was immediately taking over radio promotion and sales in the U.S. for his sing "As Your Friend" featuring Chris Brown.
Obligatory press release gush from Afrojack: "I want to take my music to the next level, both creatively and from a business point of view. Partnering up with UMG will give me the chance to do both, so I am very happy we were able to make a deal. UMG will support my releases with very strong budgets and with great people working my music around the world."
Obligatory press release gush from Hugo Langras, Afrojack's manager. "This deal is an ideal combination of Wall Recordings' strength as an independent with the worldwide marketing and distribution infrastructure that UMG can provide us with. We are very happy with this agreement, as it will allow us to take Afrojack's career as a recording artist to the next level, while safeguarding his independence and stature in the EDM world."
Let's hope the deal includes driving lessons.
News	000, Afrojack, Nick van de Wall, Universal Music Group
Afrojack Buys $240k Ferrari, Wrecks It An Hour Later
Just because you can mix records doesn't mean you know how to drive. Shortly after purchasing a brand new red Ferrari 458 Italia, Dutch DJ/producer Afrojack (a.k.a Nick van de Wall) tweeted, "I made a new friend!!!" But the friendship went sour no less than an hour later as Afrojack promptly crashed his new luxury ride. He tweeted, "Ok that sucks. Good thing we're all ok!!!! Lesson in life: don't drive a Ferrari in sh*t weather!"
After fans inquired as to why he's smiling in the picture he posted, he responded: "For everyone thats asking me why im smiling: its a car, i have a great family and just as great fans!! love all of you!"
In unrelated news Afrojack later tweeted that he's having a secret listening party for his album today in Holland. Our advice? Stick to music, Nick, and leave the driving to the experts!
News	Afrojack, Nick van de Wall
DJs Don't React to Forbes' Highest-Paid DJ List on Twitter
August 6, 2012 Darren Ressler Leave a Comment
DJs regularly take to Twitter to express their feelings — to promote an upcoming release or show, vent or share thoughts or be a little silly with their followers. With Forbes (a business new and financial publication that's become obsessed with DJ culture) recently publishing its list of highest-paid DJs (see below), we thought Twitter would be awash with commentary — both positive, negative and indifferent — from DJs.
Well, we scoured Twitter looking for opinions on the aforementioned topic and came up empty.
Aside from a congratulatory tweet from Dillon Francis and retweets of the article by Addison Groove and Funkmaster Flex, even the most opinionated jocks were silent on Forbes' list. Even more curious was that not one DJ had a word to say about DJ Pauly D of MTV's Jersey Shore fame earning an estimated $11 million.
Perhaps the old proverb is true after all: when money speaks, the truth is silent.
Forbes' List of Top-Paid DJs
1. Tiësto: $22 million
2. Skrillex: $15 million
3. Swedish House Mafia: $14 million
4. David Guetta: $13.5 million
5. Steve Aoki: $12 million
6. Deadmau5: $11.5 million
7. DJ Pauly D: $11 million
8. Kaskade: $10 million
9. Afrojack: $9 million
10. Avicii: $7 million
Features	Addison Groove, Afrojack, Avicii, David Guetta, Deadmau5, Dillon Francis, DJ, Forbes Highest-Paid DJs, Funkmaster Flex, Kaskade, Skrillex, Steve Aoki, Swedish House Mafia, Tiësto, Twitter


Amari Malone has been a member of HBCU CONNECT since Nov 12th, 2018. Amari represents Jackson State University class of 2021 and is located in the South Bend , IN area. You can connect with Amari Malone and other interesting members of HBCU CONNECT by signing up or logging in.


It's happened; we've passed the 3/4 point of our adventure! We're not exactly sure how we feel about that. We feel sadness that we've left South America behind. Disappointment that we did not get to visit Central America. Excitement about what still lies ahead, and nervous anticipation of our return home.
We've experienced a lot in these past seven and a half months. Since passing the halfway point we've travelled through Ecuador and Colombia. Both of which left lasting good impressions. We were satisfied with our month in Ecuador, although we would not rule out a third visit there in the future. Our month in Colombia however left us wanting more. A lot more. We had to speed past more than a few attractions in order to meet our shipping timeline and wished we could have lingered longer in some of the amazing places we visited. We're at peace with that. We know we can return again one day. Perhaps when we finally get around to visiting Central America.
Perhaps the most exciting thing that transpired for us in the last few months were the visits from Rob's brother Pete and mother Betty. Pete hung out with us in Ecuador for a week, including some sightseeing in Quito and a visit to the Amazon (read about it in our Ecuador Blog). He took off to the Galapagos before meeting us again for another week on the caribbean beaches of Colombia, near Tyrona National Park. Immediately after that we were treated to a visit from 'Bamma' (Grandma) in the amazing colonial city of Cartagena. It was s fun reunion. The kids were thrilled to have her explore with us, and we knew she'd also been missing the kids.
Although we made the decision to skip Central America many months ago, it was only recently that it became a reality. We were still very comfortable with that decision. Our 300-day timeline was simply not long enough to move at a comfortable pace over so much distance (600 days might have worked). Additionally, the recent outbreaks of civil unrest, first in Honduras and then in Nicaragua, made us very glad not to be adding that type of stress to our travel. We arranged shipping of the van out of Cartagena, Colombia to Veracruz, Mexico using a British agency (IVSS). It did not go exactly as planned, of course. The vessel was over four days late into Cartagena and then an extra day late into Veracruz, which left us homeless for an extra week.
Our family is still struggling a little with the vanlife. We definitely take comfort in the routines we've developed but there are still plenty of "bad days" mixed with the good. One of our theories was that we lash out at one another because of our perpetual lack personal space and privacy while living in the van. However, even while spending a week in a two-bedroom house in Veracruz we still seemed to be on each others nerves a lot. The good news is that Liam is settling down somewhat and his behaviour is now mostly appropriate for a nearly-eight-year-old. We're not sure if this is because he's finally becoming comfortable with our new lifestyle, or because he sees that the end of the trip is near. Maybe, just thinking about Disney Land is keeping him in a better mood.
Something we've noticed while driving through South America is that each country seems to have a "favourite car". A car that turns up in our mirrors at an uncanny rate. Call us paranoid, but they also seem to even favour a specific colour for that vehicle. Sometimes it was so predictable it made us laugh. We've made a list of the cars that we think epitomize each of the South American countries we visited. Unfortunately we did not think to take pictures of these "favourite cars" while we were there so we've had to steal a few pictures off the internet (our apologies for the gratuitous copyright infringement).
These legendary trucks are everywhere, and we don't blame them. The "original" 4-door ute' has a solid reputation. Rob has wanted one ever since he saw them in Asia nearly 20 years ago.
We have mixed emotions about these trucks. Rob really wishes they were available in Canada because he figures he'd probably like to own one. One the other hand, we had so many bad experiences with the uber-aggressive drivers in Argentina that we also hope to NEVER see one of these trucks ever again! Like ever.
Uruguayans really like their car/truck things, but the Renault version was the most prevalent. They seem like a sensible fit for Uruguayans; plenty of space for family and work.
Bolivia: Collectivos and Tuk Tuks!!!
We still have nightmares about the hordes collectivo buses and tuk tuks swarming around our van, honking incessantly, as we try in vain to cross any intersection in La Paz. Seemingly so close in every direction that you could reach out and change their radio station for them! Argh!!!!
These were used all over Peru as taxis, family cars, delivery trucks, schools buses, and tractors. Although Toyota only thought to install 5 seatbelts, they were really downplaying the passenger capacity because we've seen these loaded with 10 or more people, easy.
This is one example of the many Isuzu (and Suzuki) vehicles that Chevrolet markets in South America. Interestingly, we think we'd like it more if it still had its Isuzu badge on it.
It was too difficult to pin down the "favourite car" in Colombia, but there were actually several very unique, and relatively common, vehicles that stand out in our minds. For instance, the huge colourful Chiva buses that still transport people in the rural areas, or the classic Willy's Jeeps that are used as taxis in the mountainous Coffee Region. However, the one that we give our award too is the charming Renault Master. It was actually produced in Colombia from the '70s until the mid '90s and remains one of the most popular cars in Colombia, earning the nickname "Amigo Fiel" (Faithful Friend). Fun fact; because of its suspension design, it actually has a longer wheelbase on one side than the other!


text:
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (PE Conjugate)
What is a Recombinant Antibody and Why is it Important?
Recombinant antibodies offer several key advantages compared to traditional antibodies. These include superior lot-to-lot consistency, continuous supply, and animal-free manufacturing. As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis.
What is a Recombinant Antibody?
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. They are generated by immunizing an animal with an antigen to elicit an immune response. While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope.
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable.
Superior Lot-to-Lot Consistency
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results.
In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Moreover, since the recombinant antibody sequence is known, continuity of supply is assured; in situations where an antibody will be used to support large, long-term studies, this can be an especially critical factor.
Animal-free Manufacturing
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. At CST, we adhere to the Hallmarks of Antibody Validation™, six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust.
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (PE Conjugate) #13842
Flow cytometric analysis of serum-starved NIH/3T3 cells, untreated (blue) or treated with mouse platelet-derived growth factor BB (200 ng/ml, 10 min; green), using Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (PE Conjugate).
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (PE Conjugate) 13842
13842S 100 µl (50 tests)
MW (kDa)
Source/Isotype Rabbit IgG
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb #13038.
Flow Cytometry (Fixed/Permeabilized) 1:50
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Select your Protocol Flow Cytometry (Fixed/Permeabilized) PRINT
Flow Cytometry, Methanol Permeabilization Protocol for Directly Conjugated Antibodies
All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit (Methanol) #13593, or individually using the catalog numbers listed below.
1X Phosphate Buffered Saline (PBS): To prepare 1 L 1X PBS: add 100 ml 10X PBS (#12528) to 900 ml water mix.
4% Formaldehyde, Methanol-Free (#47746)
100% Methanol (#13604): Chill before use
Antibody Dilution Buffer: Purchase ready-to-use Flow Cytometry Antibody Dilution Buffer (#13616), or prepare a 0.5% BSA PBS buffer by dissolving 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
B. Fixation
NOTE: Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
NOTE: Antibodies targeting CD markers or other extracellular proteins may be added prior to fixation if the epitope is disrupted by formaldehyde and/or methanol. The antibodies will remain bound to the target of interest during the fixation and permeabilization process. However, note that some fluorophores (including PE and APC) are damaged by methanol and thus should not be added prior to permeabilization. Conduct a small-scale experiment if you are unsure.
Pellet cells by centrifugation and remove supernatant.
Resuspend cells in approximately 100 µl 4% formaldehyde per 1 million cells. Mix well to dissociate pellet and prevent cross-linking of individual cells.
Fix for 15 min at room temperature (20-25°C).
Wash by centrifugation with excess 1X PBS. Discard supernatant in appropriate waste container. Resuspend cells in 0.5-1 ml 1X PBS. Proceed to Permeabilization step.
Alternatively, cells may be stored overnight at 4°C in 1X PBS.
C. Permeabilization
Permeabilize cells by adding ice-cold 100% methanol slowly to pre-chilled cells, while gently vortexing, to a final concentration of 90% methanol.
Permeabilize for a minimum of 10 min on ice.
Proceed with immunostaining (Section D) or store cells at -20°C in 90% methanol.
D. Immunostaining
NOTE: Count cells using a hemocytometer or alternative method.
Aliquot desired number of cells into tubes or wells. (Generally, 5x105 to 1x106 cells per assay.)
Wash cells by centrifugation in excess 1X PBS to remove methanol. Discard supernatant in appropriate waste container. Repeat if necessary.
Resuspend cells in 100 µl of diluted primary antibody, prepared in Antibody Dilution Buffer at a recommended dilution or as determined via titration.
Incubate for 1 hr at room temperature. Protect from light.
Wash by centrifugation in Antibody Dilution Buffer or 1X PBS. Discard supernatant. Repeat.
Resuspend cells in 200-500 µl of 1X PBS and analyze on flow cytometer.
posted July 2009
Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of Akt1 protein only when phosphorylated at Thr308. This antibody also recognizes endogenous levels of Akt2 protein when phosphorylated at Thr309 or Akt3 protein when phosphorylated at Thr305.
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr308 of human Akt1 protein.
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3K/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin-dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).t
Franke, T.F. et al. (1997) Cell 88, 435-7.
Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
Franke, T.F. et al. (1995) Cell 81, 727-36.
Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
Jacinto, E. et al. (2006) Cell 127, 125-37.
Cardone, M.H. et al. (1998) Science 282, 1318-21.
Brunet, A. et al. (1999) Cell 96, 857-68.
Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
Cross, D.A. et al. (1995) Nature 378, 785-9.
Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
Select Your Pathway/Protein AMPK Signaling Adherens Junction Dynamics Alzheimer's Disease Angiogenesis Apoptosis Regulation B Cell Receptor Signaling ESC Pluripotency and Differentiation ErbB/HER Signaling G1/S Checkpoint IL6 Signaling Inhibition of Apoptosis Insulin Receptor Signaling Microtubule Dynamics Mitochondrial Control of Apoptosis NF-kB Signaling PI3K/Akt Signaling Protein Kinase C Signaling T Cell Receptor Signaling TGF-ß Signaling Translation: eIF4E and p70S6K Warburg Effect mTOR Signaling Protein: P31749 Protein: P31751 Protein: Q9Y243
UniProt ID: P31751 , Q9Y243 , P31749
Entrez-Gene Id: 208 , 10000 , 207


text:
The Greek American Heritage Society of Philadelphia
GAHSP
Home » Projects » Photo Tour 2019 » Sevoula Zagoraiou
Sevoula Zagoraiou
Photo Tour 2019
Sevoula Zagoraiou Siren of the Greek Islands
Sevoula Zagoraiou was born on the northernmost island of the Greek Cyclades archipelago, called Andros, to Evangelos Zagoraiou and Litsa Zagoraiou-Kekempanou. Inspired by her years of growing up on the ancient island and listening to the sweet melodies of the violin played by her father, a professional musician, she fell in love with the type of Greek music known as "Nisiotika" (music from the islands).
In 1999, she left her home and arrived in the United States, settling in New Jersey. In 2000, she formed the band "Thalassa" and immediately began singing for various Greek affairs throughout the area, becoming well-known for her mastery of island music.
She also performs "Laika," or modern music, and has performed all over the east coast of the United States as well as in Greece, with many famous musicians. She has recorded many original songs, including "Zalizoume," "Tha se trelano to'xo," "To Neo Superferry," and "I diki mou Andros."
Asked why she sings, Sevoula answered, "I sing to keep the tradition alive for the next generation and to honor those that have gone before." Today, you can find her singing with her band at Greek Festivals, clubs, restaurants, and weddings around the Delaware Valley.
She has one daughter, Maria Axarli Franks.
Subscribe to our free newsletter and stay updated on our latest projects and events.
The Heritage Center
Heritage through Sports
The Greeks of Reading
© 2002-2020 The Greek-American Heritage Society of Philadelphia. All Rights Reserved.