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SDU About SDU Departments and Centres Department of Biochemistry and Molecular Biology Research Principal investigators Martin Røssel Larsen
The group of Martin R. Larsen is focusing on the application of biological mass spectrometry in proteomics, especially the characterization of post-translational modifications in proteins and their influence on biological systems. The group is working with a large variety of biological systems and human diseases through collaborations with both academics on Universities and hospitals, and industry.
The primary focus in the group is to develop new and efficient quantitative mass spectrometric and affinity chromatographic strategies for the characterization of phosphorylated and glycosylated proteins from highly complex mixtures and apply these strategies to the study of various biological systems. The current larger projects in the group are:
- Development of large scale quantitative strategies for the identification and characterization of phosphorylated and sialylated proteins from low amount of cell material (e.g., primary cells).
- Characterization of the phosphorylation-dependent calcium signaling in nerve terminals after various stimuli, i.e., depolarization.
- Characterization of phosphorylation dependent signaling events in pancreatic beta-cells exposed to various pro-inflammatory cytokines such as Interleukine-1β.
- Characterization of altered glycosylation (i.e., sialylation) on surface proteins of cancer cells and their influence on metastasis.
- Characterization of microparticles and exosomes from apoptotic cells and body-fluids
- Membrane proteomics and the characterization of phosphorylated and sialylated proteins in membranes.
- Kinomics - the study of kinases and their presence and activity in the cell using kinase inhibitor affinity purification and mass spectrometry. Characterization of kinases in nerve-terminals.
- Biomarker discovery with the focus on glycosylated proteins in various body-fluids such as plasma, serum and cerebrospinal fluid (type 2 diabetes, Alzheimers Disease and other CNS diseases)
Beside the above mentioned projects we have a large number of small projects mainly through external collaborations.
For more information on the different areas and projects please see extended CV of Martin R. Larsen
Head of research: Professor , PhD Martin Røssel Larsen
Postdoc Taewook Kang
PhD student Eva Bang Harvald
PhD Student Marie Sejberg Øhlenschlæger
PhD fellow Komal Kumari Mandal
PhD fellow Inga Boll
Academic staff/project coordinator Arkadiusz Nawrocki
Academic staff Pia Jensen
Martin Røssel Larsen is also head of Centre for Clinical Proteomics.
1: Methodological development for modification specific proteomics on biological systems.
My research team is at the cutting edge of method development for characterizing PTMs such as phosphorylation and glycosylation in immortalized cells in culture. Having developed several methods for assessing modified peptides from complex mixtures lately we are very confident that we only observe the tip of the iceberg and do not detect the transient or low abundant biologically important modifications. In addition, recent results obtained in my group suggest that the current methods for PTMs characterization are not applicable for true in vivo studies using primary cells originating directly from animals or humans. For example investigating the phosphoproteome of a cell after external stimulation are mostly performed only using cell cultures that have been serum-starved to reduce the basal phosphorylation caused by growth factors in the medium. Serum-starvation is frequently not applicable to primary cells and tissues which undergo apoptosis under such culture conditions. Other factors also influence the analysis of primary cells and tissues, including the presence of extracellular matrix, mixed cell populations and the significantly reduced available sample for microanalysis. We speculate that not only phosphorylation but all kind of PTMs will be much more challenging to characterize from true in vivo biological systems and that current methods for analyzing PTMs are not appropriate for true in vivo studies. We have therefore initiated a focused research program aiming at developing new and more sensitive mass spectrometric methods for the assessment of PTMs from primary cells, tissues and body-fluids, biological material that cannot be manipulated prior to analysis. We will combine the newly developed affinity enrichment methods with extensive multidimensional separation methods in order to achieve the most sensitive comprehensive technique for phosphoproteimics and glycomics.
Another problem in current PTM analysis is the quantitative assessment of different PTMs in proteins. After identifying for example phosphorylation sites, the next obvious step is to quantify the changes in phosphorylation in different situations. We will continue optimizing methods for more complex samples and develop new methods for accurate quantification of PTMs from very small amount of material using stabile isotope labelling in combination with nano-purification techniques and accurate mass spectrometry.
2: Characterization of the molecular mechanism of Synaptic Vesicle Endocytosis.
There are many billions of neurons in the human brain, each with the ability to influence many other cells. An average neuron will make approximately 10,000 contacts, or synapses, with other neurons while receiving a similar input itself. Therefore, sophisticated mechanisms are essential to enable efficient communication within this highly complex system. These mechanisms are functionally localized at synapses which are the contact points between neurons. The presynaptic side of the synapse (the presynaptic nerve terminal) is the focus of this study. Although many kinds of synapses exist within the brain, they can be divided into two distinct populations: electrical synapses and chemical synapses. In the brain, the number of electrical synapses is significantly lower than the number of chemical synapses. Electrical synapses permit direct, passive flow of electrical current from one neuron to another and therefore allow much faster communication than chemical synapses. In contrast, chemical synapses use small signalling molecules, or neurotransmitters (e.g. acetylcholine, dopamine or glutamate), to enable cell-to-cell communication. Neurotransmitters are stored in small organelles termed synaptic vesicles (SVs), which are normally at rest bound to the cytoplasmic cytoskeleton. Upon depolarization, neurotransmitters are released by the presynaptic terminal into the space between the pre- and postsynaptic cells, the synaptic cleft, and bind and activate specific receptors at the post-synaptic membrane to initiate signalling events. After release of neurotransmitters the SV is retrieved back into the nerve terminal. This overall process is called synaptic transmission.
In the chemical synapse, the release of neurotransmitters is triggered by the influx of calcium through ion-channels in the presynaptic membrane. An increase of Ca2+ in the presynaptic nerve terminal leads to a multitude of molecular events resulting in release of neurotransmitters to the synaptic cleft. SVs are firstly released from the cytoskeleton and transported to the plasma membrane at the synapse where they fuse with the presynaptic plasma membrane to release their content into the synaptic cleft – this is called exocytosis. After release of the neurotransmitter molecules, the used SVs are retrieved, also via a Ca2+-regulated process, back into the pre-synaptic cell for another round of neurotransmitter filling and exocytosis – this is called endocytosis. Both exo- and endocytosis are controlled by phosphorylation and dephosphorylation of synaptic proteins by various kinases and phosphatases that become activated as a result of Ca2+ influx.
Previously we have in close collaboration with Prof Phillip J. Robinson, Children's Medical Research Institute (CMRI), Sydney, Australia found more than 50 phosphorylation sites on 4 different proteins that participate in the SV endocytosis. In future projects we will (1) complete a full map of phosphorylated proteins in the synaptosomes and quantify their changes during depolarization, (2) elucidate the biological role of these phosphorylation sites in protein interaction and complex assembly, (3) quantify the changes of phosphorylation of synaptosomal proteins in neurological diseases e.g., epilepsy.
3: Unrevealing the pathogenesis of Type 1 Diabetes Mellitus.
Accumulating evidence supports a role for cytokines, especially interleukin 1b (IL-1b), released during this inflammation, in the pathogenesis of T1DM. IL-1b, either alone, or in combination with other cytokines (tumour necrosis factor-a and interferon g) induces the production of toxic nitric oxide (NO) free radicals by the beta-cell itself, which leads to beta-cell self-destruction. We have initiated a research project elucidating the phosphorylation events involved in interleukin 1b signal transduction. We have discovered from low amount of material a large number of phosphorylated peptides and new phosphorylation sites that have not been characterized and quantified before. This study will be extended to elucidate the phosphorylation events in the signal transduction pathways of the three pro-inflammatory cytokines; IL-1b, TNFa and INFg and the crosstalk between those pathways.
Knowledge on the exact molecular mechanisms used by each cytokine in signalling cascades is still very limited. The phosphorylation events of the proteins in the downstream signalling pathways (STAT1, IKK-NFκB and MAPK) are extensively characterized primarily because they are shared by many other signalling pathways (e.g., EGF and PDGF). However, information on the regulatory phosphorylation events of the proximal membrane complexes are relatively limited. Knowledge of the precise regulation of these complexes by phosphorylation is essential to the future development of new pharmaceuticals designed to stop the development of T1DM by blocking the phosphorylation dependent activation (e.g., by inhibiting an involved kinase). Drugs inhibiting proteins later in the pathway will consequently effect many other functions in the cell and thus cause many unwanted side effects.
4: Investigation of the influence of altered protein glycosylation on cancer development and metastasis.
Protein glycosylation is among the most common PTMs known in nature. Glycosylation is difficult to analyze by any biochemical methods due to the chemically very similar monosaccharide building blocks and pronounced glycosylation site heterogeneity and micro-heterogeneity of the carbohydrate chains with respect to branching patterns and monosaccharide composition. The biological role of protein glycosylation varies from conformational stability, protection against degradation, to molecular and cellular recognition in for example development, growth and cellular communication.
It is well known that glycosylation patterns in chronic disease can be highly aberrant as a consequence of changes in the expression or activity of glycosyltransferases or other factors affecting the glycan biosynthesis. Many extracellular and surface glycoproteins contain sialic acid (SA) as the monosaccharide located on the reducing end of the glycans. These can either be located on the glycoproteins as monomer, dimers, trimers or as large polysialic acid structures comprising of more than 200 SA residues. It has previously been demonstrated that cancer development and staging may be associated with a significant over-representation of SA on the cell surface glycoproteins of cancer cells compared to normal cells (e.g., [Dall'Olio, F., Chiricolo, M. Sialyltransferases in cancer. Glycoconj J. 2001, 18(11-12), 841-50]). The transformation of a tumor from benign to malignant is associated with increased SA content on the surface glycoproteins. Also it is well known that the amount of free SA and lipid and protein bound SA is elevated in plasma from cancer patients compared to healthy individuals.
Based on the methods developed in my group for selective isolation of sialylated peptides we have initiated a research program together with Professor Dr. Med. Henrik Ditzel, Medical Biotechnology Center, Odense University Hospital, Denmark and Dr. Med. Niels H.H. Heegaard, State Serum Institute, Denmark to investigate the role of glycosylation, especially sialylation, in cancer development. This is primarily a basic research project aiming at understanding the function of sialic acids and glycosylation in relation to cancer. In this project we will correlate the expression and activity of sialyltransferases in cancer cells and "normal" cells with the sialylation degree of the surface glycoproteins using stabile isotope labelling, TiO2 chromatography, hydrophilic interaction chromatography and mass spectrometry.
5: Biomarker discovery program
The aim of these clinical proteomics project is to investigate the possibility of detecting modified biomarkers (e.g., phosphorylated, glycosylated, oxidized etc.) indicative of a recent onset of T1DM and cancers in plasma. We have taken two different approaches for this research.
The primary focus of this research is to investigate if glycosylated proteins, especially sialylated glycoproteins can be used as biomarkers for a number of diseases including Type 2 diabetes, cancers, Alzheimers Disease and other CNS related diseases.
Manuscripts under revision or in preparation
75. Jensen SS, Le Bihan MC, Lainé J, McGuire J, Pociot F, Larsen MR. Characterization of Membrane-shed Microvesicles from Cytokine-Stimulated Beta-Cells using Proteomics Strategies. in preparation.
74. Jensen SS, Bahl JMC, Heegaard NHH, Larsen MR. Characterization of the Human Cerebrospinal Fluid N-linked sialioproteome by Titanium Dioxide Affinity Chromatography and Mass Spectrometry. in preparation.
73. Solis N., Larsen M.R. and Cordwell S.J. Improved accuracy of cell surface shaving proteomics in Staphylococcus aureus using a false positive control. Submitted to Proteomics.
72. Bache, N., Graham, M.E., McCluskey, A., Robinson, P.J., Larsen, M.R. N-terminally SPITC labelling of phosphorylated peptides and sialylated glycopeptides directly on Titanium dioxide micro-columns combined with MALDI tandem Mass Spectrometry. Submitted to Analytical Chemistry.
71. Chircop, M., Sarcevic, B., Larsen M.R., Malladi, C.S., Graham, M.E., Zavortink M., Chau, N., Smith, C.M., Quan, A., Anggono, V., Graham, M.E. and Robinson P.J. Mitotic phosphorylation of dynamin II at serine-764. In preparation.
Articles published or accepted
70. Rewitz KF, Larsen MR, Lobner-Olesen A, Rybczynski R, O'Connor MB, Gilbert LI. A phosphoproteomics approach to elucidate neuropeptide signal transduction controlling insect metamorphosis. Insect Biochem Mol Biol. 2009, 39(7), 475-83.
69. Thingholm, T.E., Jensen, O.N. and Larsen M.R. Analytical strategies for phosphoproteomics. Proteomics. 2009, 9(6), 1451-68.
68. Thingholm, T.E. and Larsen, M.R. Enrichment and separation of mono- and multi-phosphorylated peptides using Sequential elution from IMAC prior to mass spectrometric analysis. Methods Mol Biol. 2009, 527, 57-66.
67. Thingholm, T.E., Jensen, O.N. and Larsen, M.R. The use of titanium dioxide micro-columns to selectively isolate phosphopeptides from proteolytic digests. Methods Mol Biol. 2009, 527, 67-78.
66. Krintel, C., Osmark, P., Larsen, M.R., Resjö, S., Logan, D. and Holm, C. Ser649 and Ser650 are the major determinants of protein kinase A-mediated activation of human hormone-sensitive lipase against lipid substrates. PLoS One. 2008, 3(11), e3756.
65. Olsen, B.B., Larsen, M.R., Boldyreff, B., Niefind, K. and Issinger O-G. Exploring the intramolecular phosphorylation sites in human Chk2. Mutat Res. 2008, 646(1-2):50-9.
64. Bahl, J., Jensen, S.S., Larsen M.R., Heegaard, N.H.H. Characterization of the human cerebrospinal fluid phosphoproteome by titanium dioxide affinity chromatography and mass spectrometry. Anal Chem. 2008, 80(16):6308-16.
63. Thingholm, T.E., Larsen, M.R., Ingrell, C.R., Kassem, M., Jensen O.N. TiO(2)-based phosphoproteomic analysis of the plasma membrane and the effects of phosphatase inhibitor treatment. J Proteome Res. 2008, (8), 3304-13.
62. Chrétien A, Dierick JF, Delaive E, Larsen MR, Dieu M, Raes M, Deroanne CF, Roepstorff P, Toussaint O. Role of TGF-beta1-independent changes in protein neosynthesis, p38alphaMAPK, and cdc42 in hydrogen peroxide-induced senescence-like morphogenesis. Free Radic Biol Med. 2008, 44(9), 1732-51.
61. Craft, G.E., Graham, M.E., Bache, N., Larsen, M.R., Robinson, P.J. The in vivo phosphorylation sites in multiple isoforms of amphiphysin I from rat brain nerve terminals. Mol Cell Proteomics. 2008, 7(6), 1146-61.
60. Larsen, A.K., Lametsch, R., Elce, J.S., Larsen, J.K., Thomsen, B., Larsen, M.R., Lawson, M.A., Greer, P.A., Ertbjerg, P. Genetic disruption of calpain correlates with loss of membrane blebbing and differential expression of RhoGDI-1, cofilin and tropomyosin. Biochem J. 2008, 411(3), 657-66.
59. Thingholm, T.E., Jensen O.N., Robinson P.J. Larsen, M.R. SIMAC - A phosphoproteomic strategy for the rapid separation of mono-phosphorylated from multiply phosphorylated peptides. Mol Cell Proteomics. 2008, 7(4), 661-71.
58. Jensen, S.S. and Larsen, M.R. Evaluation of phosphopeptide enrichment procedures. Rapid Commun Mass Spectrom. 2007, 21(22), 3635-3645.
57. Larsen, M.R., Jensen, S.S., Jakobsen, L.A., Heegaard, N.H. Exploring the Sialiome using Titanium Dioxide chromatography and Mass Spectrometry. Mol Cell Proteomics. 2007, 6(10), 1778-1787.
56. Banuelos S, Omaetxebarria MJ, Ramos I, Larsen MR, Arregi I, Jensen ON, Arizmendi JM, Prado A, Muga A. Phosphorylation of both nucleoplasmin domains is required for activation of its chromatin decondensation activity. J. Biol Chem. 2007, 282(29), 21213-21.
55. Palmisano G, Sardanelli AM, Signorile A, Papa S, Larsen MR. The phosphorylation pattern of bovine heart complex I subunits. Proteomics. 2007, 7(10), 1575-83.
54. Jovceva E, Larsen MR, Waterfield MD, Baum B, Timms JF. Cofilin phosphorylation: a homeostatic sensor of lamellipodial actin levels during protrusion dynamics. J Cell Sci. 2007, 120, 1888-97.
53. Graham ME, Anggono V, Bache N, Larsen MR, Craft GE, Robinson PJ. The in vivo phosphorylation sites of rat brain dynamin I. J Biol. Chem. 2007, 18, 282(20), 14695-14707.
52. Thingholm TE., Jorgensen TJD., Jensen ON and Larsen MR. Highly selective enrichment of phosphorylated peptides using titanium dioxide. Nature Protocols, 2006, 4, 1929-1935.
51. Labbate M, Zhu H, Thung L, Bandara R, Larsen MR, Willcox MD, Givskov M, Rice SA, Kjelleberg S. Quorum sensing regulation of adhesion in Serratia marcescens MG1 is surface dependent. J Bacteriol. 2007, 189(7):2702-11.
50. Verrills NM, Pou'ha S, Liu M, Liaw TYE, Larsen MR, Ivery M, Marshall G, Gunning P, Kavallaris M. (2006) Alterations in gamma-actin mediate resistance to tubulin-targeted drugs in childhood leukemia. J Natl Cancer Inst. 2006, 98(19), 1363-74.
49. Larsen M.R., Trelle M.B., Thingholm T.E. and Jensen O.N. Analysis of posttranslational modifications of proteins by tandem mass spectrometry. Biotechniques. 2006, 40(6), 790-8.
48. Ferreira S, Hjerno K, Larsen MR, Wingsle G, Larsen P, Fey S, Roepstorff P, Pais MS. Proteome Profiling of Populus euphratica Oliv. Upon Heat Stress. Ann Bot (Lond). 2006, 98(2):361-77.
47. Stelzer, S., Egan, S., Larsen, M.R., Bartlett, D.H., and Kjelleberg, S. Unravelling the role of the ToxR-like transcriptional regulator WmpR in the marine antifouling bacterium Pseudoalteromonas tunicata. Microbiology. 2006, 152, 1385-94.
46. Karlsen AE, Larsen ZM, Sparre T, Larsen MR, Mahmood A, Størling J, Roepstorff P, Wrzesinski K, Larsen PM, Fey S, Nielsen K, Heding P, Ricordi C, Johannesen J, Kristiansen OP, Christensen UB, Kockum I, Luthman H, Nerup J, and Pociot F. Immune-mediated beta-cell destruction in vitro and in vivo - a pivotal role for galectin-3. Biochem Biophys Res Commun. 2006, 26, 344(1), 406-15.
45. Rinalducci, S., Larsen, M.R., Mohammed, S, Zolla L. Novel protein phosphorylation site identification in spinach stroma membranes by titanium dioxide microcolumns and tandem mass spectrometry. J Proteome Res. 2006, 5(4):973-82.
44. Lametsch, R., Kristensen, L., Larsen, M.R., Therkildsen, M., Oksbjerg, N., and Ertbjerg, P. Changes in the muscle proteome following compensatory growth in pigs. J Anim Sci. 2006, 84(4), 918-24.
43. López-Villar E., Monteoliva L., Larsen M.R., Sachon E., Mohammed S., Pardo M., Pla J., Gil C., Roepstorff P. and Nombela C. Genetic and proteomic evidence supports cell surface localization for yeast enolase. Proteomics. 2006, Suppl 1:S107-18.
42. Larsen, M.R., Thingholm, T.E., Jensen, O.N., Roepstorff, P and Jorgensen T.J.D. Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol Cell Proteomics 2005, 4(7), 873-86.
41. Sparre T, Larsen MR, Heding PE, Karlsen AE, Jensen ON, Pociot F. Unravelling the Pathogenesis of Type 1 Diabetes with Proteomics – present and future directions. Mol Cell Proteomics 2005, 4(7), 873-86.
40. Larsen, M.R., Højrup P and Roepstorff P. Characterization of gel-separated glycoproteins using two-step proteolytic digestion combined with sequential micro-columns and mass spectrometry. Mol Cell Proteomics. 2005, 4(2), 107-19.
39. Kristensen, L., Larsen, M.R., Højrup, P., Issinger, O-G. and Guerra, B. Phosphorylation of the regulatory b-subunit of protein kinase CK2 by checkpoint kinase Chk1: identification of the in vitro CK2-b phosphorylation site. FEBS Lett. 2004, 569(1-3):217-23.
38. Fleckenstein B., Qiao S-W., Larsen MR., Jung G., Roepstorff P., and Sollid LM. Molecular basis of covalent complexes between tissue transglutaminase and gliadin peptides. J Biol Chem. 2004, 279(17), 17607-16.
37. Hjerrild M, Stensballe A, Rasmussen TE, Kofoed CB, Blom N, Sicheritz-Ponten T, Brunak S, Larsen MR, Jensen ON and Gammeltoft S. Identification of phosphorylation sites in protein kinase A substrates using artificial neural networks and mass spectrometry. J Proteome Res. 2004, 3(3), 426-33.
36. Larsen MR, Graham M, Robinson PJ and Roepstorff P. Improved mass spectrometric detection of hydrophilic phosphopeptides using graphite powder micro-columns. Mol Cell Proteomics 2004, 3, 456-465.
35. K. Nielsen, T. Sparre, M.R. Larsen, S.J. Fey, P. Mose Larsen, P. Roepstorff, J. Nerup and A.E. Karlsen. Protein expression changes during [[beta]]-cell maturation are associated with IL-1[[beta]] sensitivity. Diabetologia, 2004, 47(1), 62-74.
34. Sparre T, Reusens B, Cherif H, Larsen MR, Roepstorff R, Fey SJ, Mose Larsen P, Remacle C and Nerup J. Proteome analysis of islets of Langerhans exposed to intrauterine low protein diet. Diabetologia, 2003, 46(11), 1497-511.
33. Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ. Cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol. 2003, 5(8), 701-10.
32. Larsen MR, Cordwell SJ, Roepstorff P. Graphite powder as an alternative to reversed phase material for desalting and concentration of peptide mixtures prior to mass spectrometric analysis. Proteomics 2002, 2, 1277–1287.
31. Cordwell SJ, Larsen MR, Cole RT, Walsh BJ. Comparative proteomics of Staphylococcus aureus and the response of methicillin resistant and sensitive strains to Triton X-100. Microbiology 2002, 148(Pt 9), 2765-81.
30. Shaw AC, Larsen MR, Roepstorff P, Christiansen G and Birkelund S. Identification and charcterization of a novel Chlamydia trachomatis reticulate body protein. FEMS Microbiol Lett. 2002, 212(2), 193-202.
29. Shaw AC, Vandahl BB, Larsen MR, Roepstorff P, Gevaert K, Vandekerckhove J, Christiansen G and Birkelund S. Characterization of a secreted Chlamydia Protease. Cell Microbiol. 2002, 4(7), 411-24.
28. Skylas DJ, Cordwell SJ, Hains PG, Larsen MR, Basseal DJ, Walsh BJ, Blumenthal C, Rathmell W, Copeland L and Wrigley CW. Heat shock of wheat during grain filling: characterisation of proteins associated with heat-tolerance using a proteome approach. Journal of Cereal Science 2002, 35, 175-188.
27. Janssen MJFW, Van Voorst F, Ploeger GEJ, Larsen PM, Larsen MR, Kroon AIPM, and Kruijff B. Identification of the interaction between phosphatidylcholin and the mitochondrial glycerol-3-phosphate dehydrogenase from yeast (Gut2p) by photolabeling. Biochemistry 2002 41(18), 5702-5711.
26. Pedersen SK, Christiansen J, Hansen TO, Larsen MR, Nielsen FC. Human insulin-like growth factor II leader 2 mediates internal initiation of translation. Biochem J 2002, 363(Pt 1), 37-44.
25. Shaw AC, Gevaert K, Demol H, Hoorelbeke B, Vandekerckhove J, Larsen MR, Roepstorff P, Holm A, Christiansen G, Birkelund S. Comparative proteome analysis of Chlamydia trachomatis A, D and L2. Proteomics, 2002, 2, 164-186.
24. Larsen MR, Sorensen GL, Fey SJ, Larsen PM, Roepstorff P. Phospho-proteomics: Evaluation of the use of enzymatic de-phosphorylation and differential mass spectrometric peptide mass mapping for site specific phosphorylation assignment in proteins separated by gel electrophoresis. Proteomics 2001, 2, 223-238.
23. Sparre T, Nielsen K, Mose Larsen P, Fey SJ, Larsen MR, Andersen HU, Roepstorff P, Pociot F, Karlsen AE, Nerup J. Proteome analysis used in the discovery of molecular mechanisms involved in the pathogenesis of Type 1 Diabetes Mellitus. Proceedings of the Swiss Proteomics Society 2001, Functional Proteomics, ISBN 2-88476-002-4, 2001, 47-52
22. Larsen MR, Larsen PM, Fey SJ, Roepstorff P. Characterization of stress induced processing of enolase 2 from Saccharomyces cerevisiae by 2-D gel electrophoresis and Mass spectrometry. Electrophoresis 2001, 22, 566-575.
21. Larsen PM, Fey SJ, Larsen MR, Nawrocki A, Andersen HU, Kahler H, Heilmann C, Voss MC, Roepstorff P, Pociot F, Karlsen AE, Nerup J. Proteome analysis of IL-1b induced changes in protein expression in rat islets of Langerhans. Diabetes 2001, 50(5), 1056-63.
20. Jensen HH, Hjerrild M, Guerra B, Larsen MR, Hojrup P, Boldyreff B. Phosphorylation of the Fas associated factor FAF1 by protein kinase CK2 and identification of serines 289 and 291 as the in vitro phosphorylation sites. Int J Biochem Cell Biol 2001, 33(6), 577-89.
19. Brymora A, Valova VA, Larsen MR, Roufogalis BD, Robinson PJ. The brain exocyst complex interacts with RalA in a GTP-dependent manner: Identification of a novel mammalian Sec3 gene and a second Sec15 gene. J Biol Chem. 2001, 276(32), 29792-29797.
18. Powell KA, Valova VA, Malladi CS, Jensen ON, Larsen MR, Robinson PJ. Phosphorylation of Dynamin I on Ser-795 by Protein Kinase C Blocks Its Association With Phospholipids, J Biol Chem 2000, 275(16):11610-7.
17. Larsen MR and Roepstorff P. Mass spectrometric identification of proteins and characterization of their post translational modifications in proteome analysis. Fresenius J Anal Chem, 2000, 366, 677-690.
16. Heegaard NH, Larsen MR, Muncrief T, Wiik A, Roepstorff P. Heterogeneous Nuclear Ribonucleoproteins C1/C2 identified as Autoantigens by Biochemical and Mass Spectrometric Methods. Acthritis Res 2000, 2, 407-414
15. Jellinek DA, Chang AC, Larsen MR, Wang X, Robinson PJ, Reddel RR. Stanniocalcin 1 and 2 are secreted as phosphoproteins from human fibrosarcoma cells. Biochem J 2000, 350(Pt 2):453-461
14. John NE, Andersen HU, Fey SJ, Larsen PM, Roepstorff P, Larsen MR, Pociot F, Karlsen AE, Nerup J, Green IC, Mandrup-Poulsen T. Cytokine or chemically-derived nitric oxide alters the expression of proteins detected by two-dimensional gel electrophoresis in neonatal rat islets of Langerhans. Diabetes. 2000, 49(11), 1819-29.
13. Nouwens AS, Cordwell SJ, Larsen MR, Molloy MP, Gillings M, Willcox MDP and Walsh BJ. Complementing genomics with proteomics: the membrane sub-proteome of Pseudomonas aeruginosa PAO1. Electrophoresis, 2000, 21(17), 3797-3809.
12. Shaw AC, Larsen MR, Roepstorff P, Holm A, Christiansen G, Birkelund S. Mapping and identification of HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis and construction of a 2D-PAGE database. Electrophoresis 1999, 20 No. 4-5, 977-983.
11. Shaw AC, Larsen MR, Roepstorff P, Justesen J, Christiansen G, Birkelund S. Mapping and identification of interferon gamma regulated HeLa cell proteins separated by immobilized pH-gradient two-dimensional gel electrophoresis. Electrophoresis 1999, 20 No. 4-5, 984-993.
10. Byrjalsen I, Mose Larsen P, Fey SJ, Nilas L, Larsen MR, Christiansen C Two-dimensional gel analysis of human endometrial proteins: characterization of proteins with increased expression in hyperplasia and adenocarcinoma. Mol Hum Reprod 1999, (8):748-56
9. Roepstorff, P., Larsen, M.R., Rahbeck-Nielsen, H. and Nordhoff, E.; Sample preparation for matrix assisted laser desorption/ionization mass spectrometry for peptides, protein and nucleic acid, In Cell Biology: A Laboratory Handbook, Second edition, Vol. 4, Academic Press, 1998.
8. Nawrocki, A., Larsen, M.R., Podtelejnikov, A.V., Jensen, O.N., Mann, M., Roepstorff, P., Görg, A., Fey, S.J. and Larsen, P.M.; Correlation of acidic and basic carrier ampholyte and immobilised pH gradient two dimensional gel electrophoresis patterns based on mass spectrometric protein identification, Electrophoresis, 1998, 19, 1024-1035.
7. Jensen ON, Larsen MR, Roepstorff P; Mass spectrometric identification and microcharacterization of proteins from electrophoretic gels: strategies and applications, Proteins: Structure, Function and Genetic, 1998, Suppl 2(1-2), 74-89.
6. Kussmann, M.; Nordhoff, E.; Nielsen, H.R.; Haebel, S.; Larsen, M.R.; Jacobsen, L.; Jensen, C.; Gobom, J.; Mirgorodskaya, E.; Kristensen, A.K., Palm, L. and Roepstorff, P., MALDI-MS sample preparation techniques designed for various peptide and protein analytes, Journal of Mass Spectrometry, 32 (6), 593-601, 1997.
5. Fey, S.J., Nawrocki, A., Larsen, M.R., Görg, A., Roepstorff, P., Skews, G.N., Williams, R. and Larsen, P.M.; Proteome analysis of Saccharomyces cerevisiae: A methodological outline, Electrophoresis, 18, 1361-1372, 1997.
4. Spangfort, M.D.; Ipsen, H.; Sparholt, S.H.; Aasmul-Olsen, S.; Larsen, M.R.; Mörtz, E.; Roepstorff, P. and Larsen, J.N., Characterization of purified recombinant Birch allergen, Bet v 1 with authentic N-terminus, Cloned in Fusion with Maltose-Binding Protein. Protein Expression and Purification, 8, 365-373, 1996.
3. Spangfort MD, Ipsen H, Sparholt SH, Aasmul-Olsen S, Osmark P, Poulsen FM, Larsen M, Mortz E, Roepstorff P, Larsen JN; Characterization of recombinant isoforms of birch pollen allergen Bet v 1. Adv Exp Med Biol, 409, 251-4, 1996
2. Dougherty, L.J.; Brown, E.G.; Gallon, J.R.; Larsen, M.R.; Moertz, E. and Roepstorff, P., Modification of the iron protein of Gloeothece, Nitrogenase: a mass spectrometric study. Biochemical Society Transactions, 24, 477S, 1996.
1. Blomberg, A.; Blomberg, L.; Norbeck, J.; Fey, S.; Larsen, P.M.; Larsen, M.R.; Roepstorff, P.; Degand, H.; Boutry, M.; Posch, A. and Görg, A.: Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two gel electrophoresis. Electrophoresis, 1995, 16, 1935-45.
Thingholm, T.E. and Larsen, M.R. Enrichment and separation of mono- and multi-phosphorylated peptides using Sequential elution from IMAC prior to mass spectrometric analysis. Methods Mol Biol. 2009;527:57-66, xi
Thingholm, T.E., Jensen, O.N. and Larsen, M.R. The use of titanium dioxide micro-columns to selectively isolate phosphopeptides from proteolytic digests. Methods Mol Biol. 2009;527:67-78, xi.
Selby, D.S., Larsen, M.R., Omaetxebarria, M.J. and Roepstorff, P. Sample Preparation for analysis of post-translational modifications – glycosylation. Methods in enzymology. Academic Press. ISSN: 0076-6879. 2008
Larsen, M.R. and Robinson, P.J. Phosphoproteomics. Elsevier, Analytical Chemistry series: Mass Spectrometry of Proteins, 2008. Editor: Julian Whitelegge.
Selby, D.S., Larsen, M.R., Calvano, C.D. and Jensen, O.N. Glycosylation. Methods in Molecular Biology: Functional proteomics. 2008.
Hägglund, P. and Larsen, M.R. Characterization of Glycosylated Proteins Using Micro-Columns, Enzymatic Digestion and Mass Spectrometry. Spectral Techniques in Proteomics, Edition: 1st, 2007. Editor: Sem, Daniel S.
Larsen, M.R., Laugesen, S.V. and Roepstorff, P. Proteome specific sample preparation methods for matrix assisted laser desorption/ionisation mass spectrometry. In Cell Biology: A Laboratory Handbook, Third Edition, Elsevier Science, UK, 2006, edited by Julio E. Celis.
Larsen, M.R. and Jensen, O.N. Post-translational modification of proteins. In Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics, Wiley, 2005. Editors Michael J. Dunn, Lynn B. Jorde, Peter F. R. Little and Shankar Subramaniam.
Larsen, M.R. Purification of protein and peptide analytes prior to mass spectrometric analysis. Book chapter in "Proteins and Proteomics: A Laboratory Manual" 2004 (Cold Spring Harbor Laboratory Press), pp. 451-462. Editor: Professor Richard Simpson.
Larsen, M.R. Mass spectrometric characterization of post-translationally modified proteins – Phosphorylation. Methods Mol Biol. 2004, 251, 245-62. Human Press, Totowa, New Jersey, Editor: Marie-Isabel Aguilar.


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ATSA Independent Living E...
ATSA Independent Living Expo
When18 - 19 May 2016
Websitedailylivingexpo.com.au
VenueMelbourne Showgrounds
Event TypeOther
Entry Fee0.00
Organiser Melanie Robertson
In 2016, ATSA will hold a Daily Living Expo in Melbourne Showgrounds.
In Melbourne, the exhibition will have over 100 exhibitors displaying a wide range of products and services for people with disabilities and the elderly. On display will be the latest in assistive technology, mobility solutions, pressure care, employment support, accessible recreation/holiday ideas, modified motor vehicles and a lot more.
A key feature of the Expo is the FREE Clinical Education Program – run over 2 days in rooms conveniently located next to the exhibition floor. The three track seminar program has over 20 speakers.
There is plenty of parking onsite and easy access via public transport.
Admission is free to therapists, the general public, end users & ATSA members for both events. However there is a charge for staff from suppliers who are non-exhibitors which includes admission to the Clinical Program.
Thank you, Melanie Robertson will be in touch shortly.
Back to Events 2016-05-18T00:00:00+00:00 2016-05-19T00:00:00+00:00 Australia/SydneyATSA Independent Living Expo
In 2016, ATSA will hold a Daily Living Expo in Melbourne Showgrounds.

In Melbourne, the exhibition will have over 100 exhibitors displaying a wide range of products and services for people with disabilities and the elderly. On display will be the latest in assistive technology, mobility solutions, pressure care, employment support, accessible recreation/holiday ideas, modified motor vehicles and a lot more.

A key feature of the Expo is the FREE Clinical Education Program – run over 2 days in rooms conveniently located next to the exhibition floor. The three track seminar program has over 20 speakers.

There is plenty of parking onsite and easy access via public transport.

Admission is free to therapists, the general public, end users & ATSA members for both events. However there is a charge for staff from suppliers who are non-exhibitors which includes admission to the Clinical Program.
 Melbourne Showgrounds Melanie Robertson[email protected]Venue: Melbourne Showgrounds
AVALON 2021 – Australian International Airshow and Aerospace & Defence Exposition

redpajama_set_name: RedPajamaCommonCrawl


Why we love Barrister's Bed and Breakfast?
Capra Strategy loves Sleep Barristers because of the dedication that Ken, the owner places on his business. He was clear on what his goal for the website was and he did a great job expressing that to us. By being a hands on owner he learned the program and is now free to make what changes he wants on his own site, with guidance from us when needed.
Why we love Rena Delve?
I can't say enough good things about Rena. She's passionate about helping people and that comes out loud and clear with her 10 Tools for Compassionate Management. It's a real treat when you can bring someones online vision into reality and to learn so much along the way is icing on the cake. If you find yourself in need of some assistance in the realms of leadership training, management training and specifically leading a team of millennials Rena should be your first call.
What she had to say about Capra!
Why we love Buddy Sharp?
Buddy & Karen of Buddy Sharp Salon are honestly a joy to work with. They came to the table with an open mind and a clear understanding of how they wanted their clients to see them and then let us go from there creating a new brand identity, logo and site that reflected the creativity and innovation and prestige they have created in their business.
What Karen & Buddy Sharp had to say about Capra!
"Cass and her team at Capra Strategy are truly the greatest to work with! They assisted us in re-designing our salon website with such professionalism! They were very accommodating and we appreciated their fantastic timeliness in getting everything completed effortlessly!
Why we love Lake Valley Legends?
Lake Valley Legends is an incredible Bed & Breakfast dedicated to creating memorable experiences for their guests. It was a pleasure to bring their vision to life online!
Why we love List Producer?
Paula is a visionary with a passion for making people more productive, efficient and on a path to leading their very best life. That passion is a string through everything she does online.
Technical Integration with 3rd party tools to support selling, affiliates, payments, & online courses.


text:
Disciplines Ancient World (Classics) Ancient Literature & Language
Aristophanic Comedy
by K. J. Dover (Author)
Rights: Not available in British Commonwealth
Trim Size: 5.5 x 8.25
About the Book Related Books
Professor Dover's newest book is designed for those who are interested in the history of comedy as an art form but who are not necessarily familiar with the Greek language.
The eleven surviving plays of Aristophanes are treated as representative of a genre. Old Attic Comedy, which was artistically and intellectually homogeneous and gave expression to the spirit of Athenian society in the late fifth and early fourth centuries B.C. Aristophanes is regarded primarily not as a reformer or propagandist but as a dramatist who sought, in competition with his rivals, to win the esteem both of the general public and of the cultivated and critical minority. He succeeded in this effort by making people laugh, and the book pays more attention than has generally been paid to the technical means, whether of language or of situation, on which Aristophanes' humor depends. Particular emphasis is laid on his indifference-positively assisted by the physical limitations of the Greek theatre and the conditions of the Athenian dramatic festivals-to the maintenance of continuous "dramatic illusion" or to the provision of a dramatic event with the antecedents and consequences which might logically be expected. More importance is attached to Aristophanes' adoption of popular attitudes and beliefs, to his creation of uninhibited characters with which the spectators could identify themselves, and to his acceptance of the comic poet's traditional role as a mordant but jocular critic of morals, than to any identifiable and consistent elements in his political standpoint.
The School of Rome
W. Martin Bloomer
Beyond the Second Sophistic
Tim Whitmarsh
Tales of High Priests and Taxes
Sylvie Honigman
Everyday Writing in the Graeco-Roman East
Roger S. Bagnall
Against Demagogues
Robert C. Bartlett
Migrating Tales
Richard Kalmin
Apex Omnium
R. L. Rike
The Odes
Pindar


text:
Coronavirus Updates: Please click here for our response to this evolving situation.
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Pioneering the Development of Cycloid Vibration Therapy
The Niagara Story
Niagara's patented technology began in the coalfields of Canada in the 1920's.
Miners discovered that by leaning against a vibratory coal separation machine they achieved immediate relief for their aching backs and joints.
Upon hearing this the Wettlaufer Brothers who were inventors undertook formal research to further develop this idea.
Using the mining equipment as a crude starting point the brothers developed what is now known as 'cycloid vibration technology.' (CVT) is a unique energy wave created using specialised 3-dimensional vibration motor and suspension systems. As a result of their research and development, the Wettlaufer brothers were granted a US patent in 1931.
The potential of this life-changing technology was never fully realised until Mr. Owen K. Murphy stumbled onto it in 1948 just after WWII. Murphy having served with the Medical Corps during the War envisioned a vast range of products utilising this new technology.
He was so confident of its medical and commercial potential he paid USD$150,000 for the patent and in 1949 Niagara was born. The following decades saw Niagara expand its operations where it began in Australia in 1969 and in New Zealand in 1985. Currently Niagara CVT products are distributed across various territories throughout Australia, NZ & Asia by a number of dedicated distribution partners.
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Niagara CVT devices have been used by the Australian Institute of Sports (AIS) since 2006.
Niagara Therapy is a proud supplier to the Fremantle Dockers and Geelong Cats AFL Teams along with the Brisbane Broncos, North QLD Cowboys and South Sydney Rabbitohs NRL Teams.
These clubs use Niagara CVT devices on players and management throughout their respective seasons and off-seasons.
Niagara is also committed to working with associations and organisations across a variety of health conditions. We are very proud to have become a Commercial Supporter to the Australian Spinal Research Foundation in 2020 and look forward to assisting them in the work they do with Spinal Research.
Fairyland Centres
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Warnings and Contraindications
Copyright © 2021 Niagara, All rights reserved. Niagara Medical Therapy a Division of Australian Therapeutic Solutions Pty Ltd ACN 627 762 396


Can I use carpet for the bottom of the cage?
Discussion in 'Habitat' started by Lizardian, Aug 26, 2018.
I do not have any rats but am wanting to get some soon in the near future. I already have a large metal cage set up for them, but I did have a question about what to use for the bottom of the cage. The bottom of the cage is a grate so I wanted to put something solid there so the rats don't get bumblefoot or something.
My idea was to use carpet samples to lay on top of the grate so that they would have something comfortable to walk on. My plan was to get multiple carpet squares so I can rotate the the dirty carpets for the clean carpets. For cleaning, I would take the carpet out & scrub it & then let it dry for use for the next rotation. However, I've heard that rats can get their toes stuck on some carpets. So is this a good idea?
If their toes might get caught on the carpet, another idea I had was to lay down the carpets and then put a layer of fleece on top of the carpet. That way the carpet is still underneath to provide padding, but they will be walking on the fleece instead.
you can use fleece over carpet, but rats will burrow under the fleece so they will be ion the carpet.
I used to use heavy bathmats at the bottom of the cage. That worked excellent for several years.
I would buy some tiles from the hardware store and put down a layer of those and then fleece over that. It would be super easy to clean and cooling in the heat. In the winter, more fleece can be added to keep it warmer. My biggest concern about carpet would be the smell because it can't be cleaned as well with the tough backing on it.
Your cage is fairly similar to mine because it has a pan under the grate I just took the grate out and made sure there were no large enough gaps to escape and put parts of cardboard boxes and loose fleece to play with.
If I can hardly get the smell out with a washing machine, not sure how a hose could do a thing.
They don't have to go underneath for that, urine goes through fleece, that's the point of fleece is to get it away from the rat.
The reason Uhaul pads are what I heard is supposed to be what you put under the fleece covers is because it absorbs the urine, the cause of new bumblefoot. (Though I can't afford it at the moment.) But pads are also padding.
I used to use heavy bathmats at the bottom of the cage.
Oo would you describe them in detail? Because I'm using a type of bathmat that stinks in a day just like a towel, so wondered which of the many other types of bathmat there are would work or not.
Hm, if only I could use cardboard I guess it'd be easier to throw it away each day than launder 7 sets of covers each week.
Yeah, either take out the grate or use linoleum tiles or something similar that is easy to just wipe down. Honestly carpet sounds HORRENDOUS to clean, especially if it's plush/thick.
The bathmats I used had no backing, it was all material but a heavier mat. Hard to describe.
That's okay, because it sounds like one of several types I have around, so I think I know what you mean. Thanks!


The economy reached another milestone recently as the number of job openings exceeded 7 million for the first time ever. At the same time, the number of people quitting their jobs is at a 17-year high but that's a good thing. Those numbers show we're in the best labor market in decades. People are leaving their jobs to take better ones. Is there a job interview in your immediate future? Financial planner and teacher Rob West has interviewing do's and don'ts.
Before getting into the interview advice, Rob offers some thoughts on the economy and the job market.
A big part of landing a new job is doing well in the interview process and a lot of folks get nervous about that. Rob offers some advice about this.
Next, Rob talks about salary requirements because sometimes that's the scariest part of the job interview.
What about follow-up? Is that important?
If you have a buyout of your corporation in next 4 years that will yield $230,000 and a #140,000 mortgage, where should you put the proceeds?
If you're 66 and 65 and think long-term care insurance is expensive, what are the good reasons to consider it?
If your church bought a building for around $500,000 that is currently financed with a 5 year balloon loan at 4.65% which will soon go to over 6%, should your church refinance and change the mortgage company for a fixed rate?
If your residence is provided by your employer but you've always wanted to own a home, should you used extra funds to buy something to rent out or just keep saving for retirement?
If your advisor has recommended selling and buying "put options", should you consider it?
If you've been living on cash for 10 years since a divorce, how do you reestablish credit?
If your husband was working a contract finished early and was paid a lump sum to finish that contract, how does that affect your taxes and can it be mitigated?


NEW YORK (Reuters) - Warren Buffett wants to buy more businesses to add to Berkshire Hathaway Inc's "sprawl," but cautioned it may not keep the company he has run for 50 years from evolving into something rarely used to describe it up until now: average.
In his annual letter to shareholders, Buffett on Saturday said Berkshire's huge balance sheet gives him the power to funnel capital to some of the more than 80 operating units that deserve it, while its decentralized structure makes it the "home of choice" for many businesses looking to sell.
"Berkshire is now a sprawling conglomerate, constantly trying to sprawl further," Buffett wrote. "In an operating sense, Berkshire is not a giant company, but rather a collection of large companies."
That sprawl, including 9-1/2 businesses that would on their own make the Fortune 500 - Berkshire owns half of ketchup maker H.J. Heinz Co for instance - may limit its power to outperform.
Indeed, that power has been waning.
Berkshire's book value per share, Buffett's favored growth measure, has after taxes risen less than the Standard & Poor's 500 <.SPX> index including dividends, pre-tax, in five of the last six calendar years, after dwarfing the index in the prior 44 years.
Its stock price has also slightly lagged the index since the end of 2008, the company said.
"The bad news is that Berkshire's long-term gains - measured by percentages, not by dollars - cannot be dramatic and will not come close to those achieved in the past 50 years," Buffett wrote. "The numbers have become too big. I think Berkshire will outperform the average American company, but our advantage, if any, won't be great."
Buffett still wants Berkshire to make acquisitions in the $5 billion to $20 billion range. And purchases could be even bigger if he teams with partners, such as Brazil's 3G Capital.
That firm bought the rest of Heinz and got $3 billion from Berkshire to help its Burger King unit buy Canadian donut chain Tim Hortons, creating Restaurant Brands International Inc .
Buffett said he expects to work with 3G on more activities.
Still, Berkshire is sitting on $63.27 billion of cash and its most recent purchases have been comparatively small.
They have included the AltaLink electric transmission unit in Canada, and Van Tuyl Automotive, the fifth-largest U.S. auto retailer. Berkshire also plans to acquire Procter & Gamble Co's Duracell battery unit later this year.
Once in Berkshire's stable, those companies will likely stay.
Though Buffett does shed or give up some businesses - the textile company that gave Berkshire its name was closed in 1985 - Buffett said spinoffs "make no sense," citing tax reasons and a belief that businesses are worth more within Omaha, Nebraska-based Berkshire than on their own.
Buffett said his eventual successor at Berkshire, whenever he or she takes the job - "gender should never decide" - will need to monitor those businesses closely, and as Berkshire grows larger fend off the "arrogance, bureaucracy and complacency" that can destroy seemingly indestructible companies.
"For very good reasons, business owners and operating managers with values similar to ours will continue to be attracted to Berkshire as a one-of-a-kind and permanent home," Buffett wrote, italicizing "permanent" for emphasis.


Pensacola Beach Weddings makes your beach wedding ceremony easy, and stress free. All you have to do is check out our packages page, and choose the package that fits your beach wedding decor. Reserve your date with a deposit, and then make all of your decor choices. We have over 20 different colors of material for the bamboo canopy. Over 20 different color of chair sashes, or you can choose our white deluxe wedding chairs for your guests' comfort. We have nine different ceremony choices, and a ceremony music playlist, plus more. And, if you are incorporating children into your ceremony, the sand ceremony is a great way to bring a new family together.
With our Distinctive Design custom design packages, Pensacola Beach Weddings can really showcase your wedding ceremony. With our decor for your beach wedding ceremony, and the beautiful Pensacola Beach as the backdrop, your photos will be a lasting reminder of the beauty of your wedding day.


Tonight at Woodvale is the final event for Season 32! S33 starts on Tuesday!
This is the last week of Season 32, and tonight is the final event of the season at Woodvale! Season 33 starts next Tuesday (13th December), with the Season 32 Grand Finals the following Sunday on the 18th! We then have the 20th and the 22nd on at Joondalup Sports Association and Woodvale Tavern before breaking for Christmas and New Year. We will be back on at JSA on the 3rd January, and Woodvale on the 5th!
Thanks for your support this season, and we look forward to another great Season for 33!


text:
President: Biometric passports are the key step on the way to the visa-free regime with the EU
The introduction of biometric passports of Ukrainian citizens for travelling abroad is the key step in the establishment of the visa-free regime with the EU, as stated by the President. "The EU is waiting for this step of Ukraine in order to establish the visa-free regime with us," the Head of State has noted in the service center, which issues such passports.
The President hopes that the decision on the introduction of the visa-free regime between Ukraine and the EU will be approved in May, in the course of the Eastern Partnership Summit in Riga. "Due to the introduction of biometric passports, all Ukrainians will cross the borders of Schengen Area without visas," the President said. He has informed that following the negotiations with Latvian Foreign Minister, the agreement had been achieved to include the given issue in the agenda of the Summit.
According to the Head of State, about 30 thousand passports will be issued in March and more than 200 thousand passports in April. The First Minister of the Interior Eka Zguladze has noted that the procedure of issuing such passports would be simplified and the number of centers responsible for their issuance would be increased.
Biometric passports have been issued for the citizens who have made a significant contribution to the development and strengthening of the state. The first biometric passports have been given to the team that has the right to represent Ukraine, as stated by the President.
The documents have been given to Maidan activists - Olesya Zhukovska, volunteer medic wounded by sniper; Vasyl Mykhalko, wounded by sniper on February 20, when he was helping his wounded fellow leave the shooting area; legendary "voice of Maidan", former Minister of Culture Yevhen Nishchuk.
The new passport has also been given to organizer of Pirogov mobile hospital Hennadiy Druzenko and member of the "Women's Hundred" Anna Kovalenko.
The biometric passport has been issued to immigrant from Crimea Khrystyna Mykhaylichenko – winner of international contests, who won the international piano contest in the Netherlands last week, and her mother Natalia Mykhaylichenko.
The new passports have also been given to world famous writer Andriy Kurkov, filmmaker, director of "Tribe" Myroslav Slaboshpytskyi, vocalist of Brunettes Shoot Blondes – Kyiv band whose video has become a worldwide sensation of social networks and received more than 6 million views on YouTube - Andriy Kovaliov.
The passports have also been issued to journalists Andriy Tsapliyenko and Oleksandr Argat.
Member of the "Women's Hundred" Anna Kovalenko called the issuance of biometric passports a symbolic event. "I am confident that it is a step towards our dreams," she said.
Journalist Andriy Tsapliyenko believes that these documents must become a signal for the people living in the Donbas. "I think that it is a signal for those who live in the East of Ukraine. The world is open for Ukraine and closed for the enemy," he noted.
President approved the Strategy for Sustainable Development "Ukraine-2020"


Anne believes that we each have more capacity for change than we think. Her work unlocks that potential for change, thereby enabling clients deal with more complexity and greatly improve themselves, those whose lives they touch, and potentially the world. She believes deep self-awareness is the cornerstone for development and, using techniques such as Immunity to Change and Growth Edge Coaching, helps clients see and understand how they make meaning of their experiences. She provides a supportive space for individuals and teams to explore their growing edge and thereby expand their range and potential.
Anne spent over twenty years in senior corporate roles in manufacturing and outsourced supply chain management (SMC) sectors in multinational contexts. As vice-president of SCM, she oversaw significant restructuring of supply chains in high profile client organisations in Europe, US and Japan. In 2007, she transitioned into consultancy, initially in SCM and, after further training focused on leadership development. She now supports individual executives and work teams grow their adaptive capacity. Her extensive corporate experience coupled with her understanding of human development, including her own, helps create safe spaces for clients to surface places where they are stuck and explore new possibilities. She is the coach to individuals on the Executive MBA programme and also teaches Strategic SCM and Leadership Development at the Masters level for University College Cork.
Anne holds a Bachelor of Science and a Masters in Business Administration from University College Cork. She is a certified Immunity to Change coach, since 2010. She completed CTI's Leadership programme in 2013, trained in Organisation and Relationship Systems Coaching with CRR Global in 2015, works extensively with The Leadership Circle, and is currently working to complete certification in Growth Edge Coaching.
Anne lives in a town called Carrigaline where, unusually, she lives within 500 metres of the home she grew up in. Auntie is the role she does best – travelling with her nephews and niece has been a source of great joy. She also enjoys hill-walking, gardening, travelling, and staying in touch with many friends, acquaintances and colleagues around the world.


I wrote this the last night I stayed in the relationship.
Writing this and re-reading it, made the difference of me continuing in the toxic relationship and having the focus and strength to leave.
I just wanted to share my words and thoughts.
Thank you Richard for you videos and sharing your knowledge, it's made a world of difference to the situation.
Which means that today I do not.
Not flying solo against a gale.
But instead I face constant questioning, demands for explanation and told the path I have chosen, choose today or for tomorrow is wrong.
Instead people do whatever they like, without care and face no question.
If asked how safe do I feel?
I don't want to hesitate and evaluate...and think 'sort of, some of the time'.
Not dissected, picked over, dismissed.
Without self censorship for fear of the amount of energy required to defend them.
But instead I battle to be whole and the bombardment comes from the person who shares my life.
I am forever doubted, scoffed at and told my actions and plans are ridiculous and not achievable.
The 'knocker' is within my own home, daily in my life, never far away.
Than a 'been too busy to get help this week, I will soon. I work long hours you know.
Than a 'you on about that again' everything's fine, I'm fine, counselling doesn't help. Your just over sensitive and see problems where there are none.
I only yelled, ranted and was frustrated because you put that there..
I wouldn't have reacted like that if you didn't bring that home.
The justifications, the excuses, blaming me for your reaction, not taking responsibility for one self.
I sometimes forget myself and say something out loud only to be quickly reminded how you hear it and react.
I lay in bed alone but next to you, my sleep disrupted by your constant turning and my efforts to not have you react by feeling my presence.
Instead I must be passive, until you decide I'm required to meet your needs.
But you say 'I really do love you no matter what'.
What is wrapped up in your form of love is impatience, frustration, intolerance, menacing, judgement, aggression, roaring, belittling, humiliation.
When you are loving, you are amazing to be with, so gentle, kind, open.
Sometimes out of the blue, no warning given.
Other times I realise it's coming and attempt to disperse it or avoid...sometime this works, other times not, either way it's exhausting.
hours before, that you will react and I feel apprehension at what is coming.
I have to just face the truth that this is you.
But each time the pattern repeats, I get reminded of how it is.
And each time I realise that I am trying to do something different to avoid your anger, I know it's gone too far.
Every time you have said sorry, with such conviction and the promise you will seek help and work on your issues ... And then chosen not to, I know deep down there is no desire for change, no commitment.
So I'm left in a void ...one moment knowing my way forward, next minute just uncertainty about the path ahead.
I'm so tired from the battle but somewhere inside, I know I have strength, I just have to focus and believe.
I just want a calm, peaceful home, I know it can be achieved.


text:
Blair boys' soccer defeats Clarksburg in home opener
Sept. 15, 2019, 1:51 p.m. | By Billy Scott | 1 year, 4 months ago
Goals from Ryan Halli and Walter Sosa gave the Blazers their first win of the season
The Blair boys' varsity soccer team (1-1) defeated the Clarksburg Coyotes (1-0-2) by a score of 2-0 on Sept. 11. Blair's offense came alive after a scoreless first half, with several shots on goal and two goals. Throughout the game, Clarksburg's offense couldn't capitalize on their opportunities to score. In the second half, senior Ryan Halli snuck one past the keeper for the first goal of the game. Head coach Edvin Hernandez only used a few substitutes during the game, including Halli. "Today coming in, as a substitute player, we needed that boost and [Halli] gave it to us," Hernandez said.
Photo: Junior goalkeeper Donovan Cox prepares for a goal kick. (Photo courtesy of Nathalie Molina)
Following Halli's goal, Clarksburg conceded a handball, giving senior Walter Sosa a penalty kick goal. The Blazers showed a lockdown defense, with senior captain and goalkeeper Brennan Moore's four saves and junior goalkeeper Donovan Cox's three. With their first win of the season, the team is confident that they have more to come. "I think we have a great team and a great coach and we're going to go far into the playoffs," Moore said.
The Blazers play their next home game against the Churchill Bulldogs at 5:30 p.m. on Monday, Sept. 16.
Last updated: Sept. 15, 2019, 1:51 p.m.
Tags: Walter Sosa Ryan Halli Blair Athletics Boys Soccer
Billy Scott. What's up! My name is Billy Scott (he/him), and I am a sports editor for SCO! I am a member of the Dual Enrollment Program, meaning I am in the graduating class of 2021 at both Montgomery College and at Blair. I love rap, my … More »
Blair varsity girls' basketball beats Springbrook in playoffs
Blair takes a close victory over Whitman
Blair varsity girls' basketball comes up short against Walter Johnson
Blair varsity wrestling crushes Wheaton and Einstein on senior night
Blair varsity girls basketball falls to Sherwood
Blair varsity wrestling defeats Northwood
Blair boys' basketball brings home a victory against Kennedy
Blair boys' basketball defeats Sherwood
Blair boys' soccer falls to Churchill


Last Saturday I went with some bloggers to CFW it was great to meet everyone and I had a really good time.
And we all went for a Nando's afterwards all the bloggers were so lovely and it was great to meet them all!
I love this post. It was amazing to meet you!
Thanks :) It was great meeting you too!


Nice theme with cool parallax effects and a responsive design.
Red Sky theme is a modern multipurpose WordPress theme built in one-page style. The theme is designed with a view to amazing people and to leaving a great impression on anyone looking at it.


text:
Club America Dres
Club de Fútbol América S.A. de C.V., commonly known as Club América or simply América, is a professional football club based in Mexico City, Mexico. Nicknamed Las Águilas (The Eagles), it competes in Liga MX, the top tier of Mexican football. The club was founded in 1916, and since 1959 has been owned by media company Grupo Televisa. The team plays its home games at the Estadio Azteca, the largest stadium in Latin America, and one of the largest stadiums in the world. América is one of the founding members of the Primera División. The club has a long-standing rivalry with Guadalajara, as both are the most successful and most popular clubs in the country, and are the only clubs to have never been relegated.[9] Matches between them are known as El Súper Clásico, considered to be the biggest rivalry in Mexico, and one of the biggest in the world. América also play local derbies against Cruz Azul and Club Universidad Nacional.


If a professional lets you down you need legal advice you can trust. Get an honest appraisal of your case and advice on how best to move forward from an experienced professional negligence solicitor.
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Making a claim against them for negligence can be fraught with difficulties. Some are insured for such claims but a lot are not. Most will have tried to exclude or limit their liability in the small print of their contract but there are often complex arguments about whether these limitations of liability are valid. If you do make a claim, you will often find yourself being stalled and confronted at every juncture.
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What's Missing in the Adrian Peterson Story?
What in the world was Adrian Peterson thinking when he took a switch -- specifically, a tree branch -- to his 4-year-old son? He wasn't thinking, and that's part of his problem. But there's more to this horrible situation than meets the eye.
I decided to wait nearly a week after this story broke to write this piece because I've learned that these stories are like the proverbial layers of the onion. We learn more and more as the layers peel away over the days, weeks and, sometimes, months after such a story becomes public. This story still has some legs, especially as we wait to see how the legal system will judge Peterson's action. But as is typically the case, the court of public opinion is moving at light speed to render judgment on Peterson, no doubt fueled by the Ray Rice and Greg Hardy domestic-violence incidents, and the lack of appropriate NFL policies that address domestic and family violence perpetrated by its players.
Even though this story continues to unfold, I decided to write this piece now because of what's been absent from coverage of this story -- two vital reasons Peterson acted in such a stupid, harmful manner.
First, Peterson was and still is ill prepared for being a father. Let me be clear. I'm not talking about whether he loves or genuinely cares for his son. I'm also not saying he lacks any knowledge or skill in being a father. What he clearly lacks, however, is an adequate base of knowledge and skills required of a nurturing, loving father, particularly in the area of disciplining a child.
Second, Peterson lacks even a basic understanding of the difference between discipline and punishment. I'll unpack this second reason before I return to the first one.
The evidence for this lack of understanding first appeared in a statement issued by Peterson's attorney, Rusty Hardin, who said, "Adrian is a loving father who used his judgment as a parent to discipline his son. He used the same kind of discipline with his child that he experienced as a child growing up in east Texas."
"I never imagined being in a position where the world is judging my parenting skills or calling me a child abuser because of the discipline I administered to my son... I have to live with the fact that when I disciplined my son the way I was disciplined as a child, I caused an injury that I never intended or thought would happen. I know that many people disagree with the way I disciplined my child."
Count me in with the crowd who disagrees as you notice how Peterson and Hardin harp on the notion that Peterson "disciplined" his child.
He did not discipline his child. He used a violent, fear-based, abusive form of punishment that was, tragically, used on him as a child.
That Peterson never questioned how he was punished as a child is also part of his problem.
Discipline, which originates from the Latin word "discipulus," means "to teach or guide." To punish means, no pun intended, to "penalize" someone for an offense through pain, loss, confinement, or even death. Taking a switch to a 4-year-old in today's world, or in the world of the recent or distant past, is simply a form of abusive, fear-based punishment meant to inflict pain. It in no way teaches or guides. Anyone who doesn't realize this kind of punishment can injure a child is not in his or her right mind.
Too many fathers, and parents in general, confuse discipline and punishment. They use the terms interchangeably. (Peterson and Hardin clearly fall into this camp.) They default to punishment because they either lack knowledge of effective ways to discipline their children or, if they know of alternatives; they take the easier, swifter path because, quite simply, discipline requires more work than punishment. Discipline requires skill in applying proven techniques. It also requires parents to be calm, patient, and nurturing before, during and after disciplining children, a quality some parents sorely lack. It also requires comfort with failure as parents learn how to effectively apply the techniques of child discipline and which ones work best with their children.
Moreover, when these parents punish -- and there are times when it is appropriate to punish children -- they use violent forms that are unnecessary and place their children at risk for injury, not to mention the damage these forms have on parent-child relationships. These parents lack the knowledge of effective, non-violent forms of punishment.
This specific lack of knowledge and skill in the discipline of children underscores a larger problem, particularly as it concerns fathers and our culture in general. Too many men become fathers without an inkling of how to be a good father. Our culture does a pitiful job of preparing men to be good fathers, and fathers know this. Several years ago, researchers from the University of Texas and Rutgers University conducted the most significant national study to date on fathers' attitudes on fathering. Only slightly more than half of the fathers surveyed "agreed," and less than a fourth "strongly agreed," that they felt adequately prepared for fatherhood when they first became fathers.
As this story continues to unfold, we must not only examine Peterson's culpability and the appropriateness of the actions taken by the NFL and the Vikings in punishing him. We must also examine the culpability of our society for its inadequate preparation of men to be fathers and lack of training for fathers in the knowledge and skills required to be involved, responsible, committed fathers. (National Fatherhood Initiative tried for years, with no success, to work with the NFL in providing fathering training and resources for its players.) While that latter examination doesn't absolve Peterson of his individual responsibility in abusing his child, it at least acknowledges that his action didn't occur in a vacuum. Fortunately, and encouragingly, the legal authorities and the citizens in east Texas who indicted him recognized his action for what it is. Let's hope Peterson will eventually do the same.
How were you disciplined and/or punished as a child? How does how you were raised help (or harm) your style of discipline as a parent today?
For fatherhood leaders, we're here to help you serve the dads you lead. You can subscribe to our ebook service; where we share "How to Discipline Your Child" along with other helpful topics for dads.


In this edition, we take a look at the world-leading research that CRDC is funding into scheduling for precision irrigation, and a grower-led, on-farm commercial scale irrigation trial comparing four alternative systems - furrow, overhead, drip and bankless - to see how they compare in 'real world' situations. We also take an in-depth look at the first ever cotton industry Herbicide Resistance Management Strategy - a valuable tool in industry-wide efforts to control herbicide resistance - and a look back at the 17th Australian Cotton Conference, held in August.
Download the Spotlight Spring 2014 edition here.


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Meidell: International Monetary Fund forecasts gloomy
Laif Meidell
The concept for the International Monetary Fund originated in July 1944 when 45 countries met in the town of Bretton Woods, N.H., and agreed upon an international framework of economic cooperation to be set up at the conclusion of World War II. At the time, the goal was to create a framework that would avoid the terrible policies that had contributed to the Great Depression.
The IMF was formalized in 1945 when the first 29 member countries signed its Articles of Agreement. France was the first country to borrow from the IMF shortly afterward in 1947. Today, the IMF has 188 member countries and is a specialized agency within the United Nations but with its own charter, governing structure and finances.
It keeps track of the economic health of its member countries in order to alert them to potential risks on the horizon and provide policy advice. They also lend to countries in financial difficulty, as well as provide technical assistance and training to help countries improve their economic management.
Recently, the IMF has warned that the global recovery is on shaky ground, with their two major concerns being the Federal Reserve's prospects of a tighter monetary policy and the geopolitical tensions in the Ukraine and the Middle East.
The IMF has said that growth in the first half of the year was weaker than it predicted in April and that it was likely to cut its next forecasts that are scheduled to be released in October.
As a result of the Federal Reserve's end to asset purchases next month and its move closer to raising interest rates, the IMF foresees tighter financial conditions in the emerging market countries. More specifically, the IMF expects to lower its growth forecast for Latin America as consumer confidence continues to decline in Brazil and Argentina deals with the consequence of its default in June.
For the week, our top-performing country is Argentina, with the MSCI All Argentina 25/50 index gaining 1.95 percent.
However, its longer-term trend is significantly lower, down 2.35 percent over the past three months.
Laif Meidell, CMT, is the president of American Wealth Management, a Reno-based Registered Investment Advisor, and is a subadviser to the AdvisorShares Meidell Tactical Advantage ETF (ticker: MATH), an SEC-registered fund, and can be reached at 775-332-7000 or laif@financialhealth.com. Securities offered through Foothill Securities Inc., member FINRA/SIPC. American Wealth Management is a separate entity from Foothill Securities. Performance numbers used in this article were obtained through eSignal and are not guaranteed to be accurate.


Active Listening, Second Edition, is a fully updated and revised edition of the popular three-level listening series for adult and young-adult learners of North American English. Each level offers students 16 engaging, task-based units, each built around a topic, function, or grammatical theme. Grounded in the theory that learners are more successful listeners when they activate their prior knowledge of a topic, the series gives students a frame of reference to make predictions about what they will hear. Through a careful balance of activities, students learn to listen for main ideas and details, and to listen and make inferences. New, culturally rich Expansion units include authentic interviews with students from around the world.
This series is intended for high-beginning to intermediate students. It can be used as a main text for listening classes or as a component in speaking or integrated-skills classes.
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MAD museum
In the southwest corner of the Central Park, across from the Maine Monument ( look at one of my posts) is the Columbus Circle rotary with a Columbus Monument designed by Sicilian sculptor Gaetano Russo in the center. There is the Museum of Arts and Design (MAD) on the south side of the circle( Columbus circle,2)
MAD was founded in 1956 by Aileen Osborn Webb as the Museum of Contemporary Crafts and later became the American Craft Museum. Mad is the only New York museum with an Open Studio program that enables visitors to observe and interact with artists engaged at work within programming spaces.
Columbus 2 , April 2014
From 1986 until 2008 MAD was located at 40 West 53rd Street and in September 2008 opened its doors at Columbus circle.
Columbus 2 before 2008 renovation
The seven-story Pabst Grand Circle Hotel, a combination theater, hotel and restaurant, designed by William H. Cauvet, stood at this address from 1874. In 1960 the hotel was demolished to free the space for the New York Coliseum. From 1964 to 2005 the site contained a 12 story modernist structure designed by Edward Durell Stone for Huntington Hartford, heir to the founder of A&P Supermarkets, to display his art collection. Stone was the principal designer on the Radio City Music Hall and Museum of Modern Art in New York. The building at Two Columbus Circle opened as the Gallery of Modern Art.
In 1969 the Gallery of Modern Art was closed , and the building used as an art exhibitions center for Fairleigh Dickinson University.
In 1996 the building turned thirty years old and became eligible for landmark designation. The New York City Landmarks Commission never held a public hearing on its fate and despite a serious preservation effort, the Museum of Arts & Design has radically altered the building for their occupation in 2008.
Architecture critic Nicolai Ouroussoff named the new building as one of seven structures in New York City that should be torn down because they "have a traumatic effect on the city."
Nicolai wrote: "... As a result, the facade is being utterly revamped. ... This was an atrocious betrayal of the public trust. ... A similar debate is unfolding in Berlin, where the German government plans to demolish the 1970s Palast der Republik. ... Both 2 Columbus Circle and the Berlin building represent important moments in their cities' collective memories."
The redesigned building has the same massing and geometric shape as the original, but the original white Vermont Marble has been replaced with a glazed terra-cotta and glass façade.
Museum has four floors of exhibition galleries, 150-seat auditorium and a restaurant. It also includes a Center for the Study of Jewelry, Education Center and three artists-in-residence studios. MAD is the only American museum with a gallery dedicated to contemporary and modern studio and art jewelry. Museum is open late on Thursday and Friday when you can pay what you wish.
In July 2014, the Museum of Arts and Design will inaugurate NYC Makers: The MAD Biennial, an exhibition that spotlights the creative communities across the five boroughs today.
Various spaces in the Museum are available for private events, including our 7th floor, atrium, and our 143-seat theater.
There is a modern American restaurant Robert on the top floor of the museum building with an amazing view of Columbus Circle and Central Park. There is a live jazz music almost every night. Open Table has about five hundred reviews with the more than five stars- excellent result!
Labels: Arts, Museum
Location: 2 Columbus Circle, New York, NY 10019, USA
The Statue of Liberty and its replicas. Part 1
The Morgan General Mail, Geen roof and Abraham Li...
Mermaid parade on Coney Island, 2014
C24: BANG BANG!
The Cathedral of St. John the Divine, the largest ...


During an interview with Barcelona's leading newspaper last month, Pope Francis referred to ..."big world economies that sacrifice man at the feet of the idol of money..." I suppose that money was always seen as the one key to happiness. A half century ago other things, however, were valued even more, things such as character, sacrifice and charity. There were candidates who ran for political office whose desires were to really serve their constituents. One became a teacher to help children be the best that they could be. Doctors wanted to do all that they could for the health of their patients. The goal of medical research was to prevent and remediate diseases. The sacrifices of soldiers, police and firefighters inspired all of us.
Indeed, today we know many who see their vocation as that of service. A teacher's joy is to see a student's growth not only in knowledge but also in character. Doctors, police and firefighters are most happy when lives are saved. The list goes on. But, what is troubling is the intrusion of greed into society. When a politician appeals to the selfish instincts of voters, something is very wrong. It's not right when a person wants to enter a profession because the money and benefits are pretty good and the time required for their service is not so demanding. The object of work soon becomes an easy, wealthy life.
St. John Paul II said that we were in a culture of death. Added to this is a culture of idolizing money as Pope Francis indicated. We have to see people as God sees them: His children whom He loves tremendously. Each human being is a person and not an object of gaining monetary profit. We do not like to be used and rightly so.
May we always grow in character, sacrifice and charity to be good examples and to show where true happiness lies.


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'Lone Survivor' Marcus Luttrell's Rules For Dating His Daughter (Video)
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Though the overall narrative is familiar, Robinson develops it via a lesser-known facet of the war: the fiercely independent mountain tribes that tried to avoid both Taliban and American entanglements. Remarkably, it took years for Luttrell, who credits the tribesman with saving his life, to find Gulab again. Robinson sincerely discusses the inscrutable, honor-bound, ancient Pashtun society and warrior code that guided Gulab. Doyle offers another lucid, inspiring chronicle of female empowerment and the rewards of self-awareness and renewal. More life reflections from the bestselling author on themes of societal captivity and the catharsis of personal freedom. In her third book, Doyle Love Warrior , , etc. Some stories merely skim the surface of larger issues, but Doyle revisits them in later sections and digs deeper, using friends and familial references to personify their impact on her life, both past and present. An engrossing memoir as well as a lively treatise on what extraordinary grace under extraordinary pressure looks like. The former first lady opens up about her early life, her journey to the White House, and the eight history-making years that followed.
Jump to navigation. For a father, there's nothing more unsettling than the boys his little daughter will date in the future. Dads being dads will make all kinds of rules when the girls are little, but then when the time comes, usually give in to their little princess' wishes. In a Facebook rant, he drew up a list of tasks that he would expect his daughter's suitor to undergo before he would allow the boy to meet her.
But even if the boy manages to do all those tasks with elan, he won't be home scot free. Luttrell further writes, "He gets that done then I'll let him have my cell number so they can face time while I hold the phone.
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Skip to Content. The movie wrestles with a profound moral quandary and viewers can decide what's right and wrong for themselves. While some parents will find the patriotism, sacrifice, and heart the soldiers display incredibly moving, others might be concerned that war and the military appears in too positive a light, and might come off like a recruitment movie.
Parents and caregivers: Set limits for violence and more with Plus. Join now. Add your rating See all 11 parent reviews. Add your rating See all 30 kid reviews. Common Sense Media's unbiased ratings are created by expert reviewers and aren't influenced by the product's creators or by any of our funders, affiliates, or partners.
Luttrell is dad to 3-year-old son Axe and 2-year-old daughter Addie. In his now-viral Facebook post, he lists his demands for any future suitors that might want to date Addie when she grows up. The requirements include doing yard work and interviewing with prison guards and MMA fighters. As of Monday afternoon, the post has been shared over 4, times and has almost 40, "likes.
A Parent's Guide to Bringing up Happy Sons in a World Filled with Toxic holding a gun And in , retired US Navy Seal Marcus Luttrell's Facebook post Will Spread," while another literary gem says "Rules for Dating My Daughter: 1.
And a couple of weeks ago we learned that they killed the most wanted terrorists of all, Osama bin Laden. Osama bin Laden was the mastermind behind the murders of nearly 3, Americans on Sept. During their recent mission, these Seals proved that when the peace of our great nation is threatened, we will stand up and fight. Throughout the ongoing conflicts in Iraq and Afghanistan, many other Seals have performed equally heroic deeds.
All Navy Seals are elite commandos demonstrating the epitome of what we have in this country. One such example is local Seal patriot Marcus Luttrell, who I am proud to recognize and honor for his contributions to the global war on terrorism. June 28, , is a date the Seals will forever remember. It was the worst single-day U. Marcus Luttrell, a sixth-generation country boy from Texas, survived to tell the incredible, harrowing events of that day.
He is an amazing Texas patriot and the lone survivor of an horrific gun battle that occurred in the mountains of Afghanistan. Luttrell was born in Huntsville in
Dads, we get it. His daughter is in for a long road ahead, seeing as she is only 2 years old. Marcus Luttrell is a retired Navy SEAL who wrote an extreme list of demands for boys who want to date his young daughter when she is of age.
Posted: Oct 2, BE.
Akiva J. Goldsman born July 7, is an American screenwriter , director, and producer known for his work on motion pictures and adaptations of popular novels. He also wrote more than a dozen episodes for the science fiction television series Fringe. His parents, Tev Goldsman and Mira Rothenberg, were both clinical child psychologists who ran a group home for emotionally disturbed children. Ann's School which is also in Brooklyn Heights, he received his bachelor's degree from Wesleyan University and attended the graduate fiction writing program at New York University.
Many of his connections were made at St. Goldsman has a production company at Warner Bros. He also directed the horror thriller film Stephanie , [6] with Frank Grillo in the leading role, [7] and co-wrote and produced the film adaptation of Stephen King 's The Dark Tower series, which was released on August 4, , and was one of Goldsman's post Apotheosis films.
In June , Paramount Pictures announced that Goldsman would head a team of writers and filmmakers to create a multifilm cinematic universe branching out from Hasbro's Transformers franchise. Goldsman was revealed in September to have been on the writing staff for Star Trek: Picard , revolving around the later years of the character of Jean-Luc Picard.
This post was contributed by a community member. The views expressed here are the author's own. The views expressed in this post are the author's own. Want to post on Patch? Register for a user account. Patch is a space for neighborhood news.
'Simple Rules' for dating my daughter.
Luttrell said in an HBO special about the making of the movie. It has already made more money than almost any military film released in the last decade. After Mr. Luttrell founded the Lone Survivor Foundation to provide other veterans, particularly combat veterans struggling with post-traumatic stress and other problems associated with life after the military, with the same course of treatment that helped him.
The retreats emphasize family counseling, therapy geared to children of veterans, and teaching pain management without a reliance on heavy doses of drugs. The foundation has also developed programs for soldiers who were sexually abused while serving. Leonard had participated in other veterans groups, but none like Mr. Five years earlier, Mr.
Leonard drove a Bradley fighting vehicle over a bomb, outside of Baghdad. The explosion blew off the lower half of his right leg and rolled his vehicle down an embankment into a sewage canal. Leonard spent the months that followed at Brooke Army Medical Center in San Antonio enduring multiple surgeries, quarantines and hours of physical therapy. He refused any counseling. Leonard said.
A good woman younger woman – how would you may be on laptops, right? My driveway and one destination for dating my daughter. While dads have a ban prohibiting males from the viral, and. Father's rules for dating my daughter: from the front-lines of dating, you'll have older woman – men, the middle of dating my daughter? Gisele bundchen is the usa the traditional rules for dating sites?
Read the Message From 'Lone Survivor' Marcus Luttrell That Glenn Beck Says Is 'One of the Greatest Things I've Ever Read From Any Dad'. TheBlaze. " I'll be.
Iraq War War in Afghanistan. Navy Cross [2] [3]. Luttrell was born in Houston, Texas on November 7, He began training for the U. He trained every day with his twin brother, Morgan, and others who aspired to join the Navy. Shelton trained them using various weight and endurance exercises. Luttrell enlisted in the United States Navy in March
Each branch of the military focuses on commitment, duty, honor, sacrifice, and service and others before self. This bleeds dating their life outside of the military — dating and marrying a veteran can be one of navy most rewarding things someone can do. The 7 everyday struggles of navy in the military.
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David then answered his call to duty and joined the Navy. In , David finished his Navy career and set out for north Texas to begin his career in the private world. A celebration of David's life will be held at Malone's Pub in Conroe at a later date. In lieu of flowers, the family requests donations be made to the Lung Cancer Society in David's honor.
Those wishing to post tributes to the family may do so at www. Grief Support. David Luttrell. Send Flowers. Share David's life story with friends and family. May God bless you and your As the days and weeks pass, and May your heart still be filled
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Like so much else about the late Chief Petty Officer Jon Tumilson, his running seemed at once humble and epic, gritty and glamorous, transparent and mysterious. He practiced the sport in a disciplined, measured fashion, and yet when he ran, he often seemed larger than life. Two hours later I'm driving over to Charles City, 14 miles away, to pick up a part.
I see this guy running along the shoulder of the highway. I get closer and I realize it's Jon. There he is! I couldn't believe it.
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