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DAVID MUIR is an American journalist and the anchor of ABC World News Tonight and co-anchor of the ABC News magazine 20/20, part of the news department of the ABC broadcast-television network, based in New York City. Muir previously served as the weekend anchor and primary substitute anchor on ABC's World News Tonight with Diane Sawyer, subsequently succeeding her on September 1, 2014. At ABC News, Muir has won multiple Emmy and Edward R. Murrow awards for his national and international journalism.
According to the Tyndall Report, Muir's news reporting received the most airtime in 2012 and 2013, making him one of the most visible journalists in America. World News Tonight with David Muir has become the most watched newscast in the United States of America. In 2013, TV Week called him one of the "12 to Watch in TV News". Muir was listed as one of People Magazine's Sexiest Men Alive in 2014.
When the bits of gossip needing David Muir to be involved with ABC field reporter Gio Benitez began, David was co-facilitating 20/20, and Gio was a standard journalist for the show. They were located together on different evenings out, and individuals rushed to conjecture. Neither of them affirmed their relationship.
By 2016, Gio got hitched to Tommy DiDario which closed down the gossipy for a while. David is most often seen roaming around with a man who press sources identify as his "boyfriend Sean." At the present time, the news of David Muir getting married to Sean is creating hot temperature in media news. It is speculated in some reports that he has already married to his partner Sean and living happy life with him.
And really….who wouldn't?


This little structure was built as a parish hall for JP Seddon's vast and grandiloquent 1870s church of St James next door, but when that was declared redundant in 1999 this hall became the new parish church. The notice board outside suggests that it has a busy life, which is not surprising as this little church is now the sole Anglican presence in the southern half of the Borough.
I came here on the occasion of the Historic Churches Bike Ride of 2010, and was pleased to find the new St James open, especially as the old church next door was not, even though it was also Open Heritage weekend. However, it turned out that the church was not taking part in the bike ride at all. Instead, a rather bemused workman was busy cutting up wood at the back of the church for a kitchen conversion. He told me that I was the sixth visitor since he'd started work, and this despite the fact that it was not yet midday.
He asked me to explain what the Bike Ride was, and when I showed him my signing in form he became quite interested, and even asked me if I thought he should sign the forms for visitors if any more came, but I assured him that this would probably only complicate matters.
He was quite happy for me to have a look around, although of course there was not a lot to see. The old church had a vast marble font which would be quite out of place here, and instead there is a little wooden birdbath. The former use of the building is obvious from the proscenium archway around the stage at the west end, but the curtains now serve a slightly different function, because they allow the sanctuary to be screened off so that the body of the building can be used for more secular purposes during the week. I wondered what JP Seddon would have made of it.


A 3-ingredient milkshake filled with dream-worthy flavors.
Creamy, quick and easy to throw together – this dairy-free shake is full of nutrients, healthy fats and protein. Just one glass will keep you full until lunch!
All you need to make this milkshake is frozen bananas, hazelnut butter, and almond milk. The frozen bananas lend a cool, ice-cream-like texture, while the hazelnut butter offers a subtle salty creaminess.
Homemade hazelnut butter is loaded with nutrients and a great source of omega-6 fatty acids. Since many store-bought hazelnut butters contain unhealthy additives, make sure you find one that's made from only organic hazelnuts. You can also whip up your own homemade hazelnut butter – all you need are toasted hazelnuts and a food processor to get the job done!
Place all the ingredients into a high speed blender.
Blend until smooth, about 1-2 minutes.
Pour into two glasses, top with chopped hazelnuts and raw cacao (optional). Enjoy.
Tip: Add a tablespoon of maple syrup or raw honey for a sweeter more decadent milkshake.


Great value for this beautiful 3 bedroom, 2 bath newer modular (not manufactured) home on 5.6 acres. Great kitchen with loads of cabinets, island, and laminate floor. Open to dining room. Great room with inviting fireplace. Family room offers additional living space. Lots of natural light streaming in from the many windows. 4 foot crawl space. Lean-to, fenced pasture, and blacktop frontage. 40 minutes south of Olathe.


GUESS WRIST WATCH EMPTY PRESENTATION GIFT BOX pretty little padded box with internal mirror space for rings/earrings and loose items. they do not appear to have been used but they each have a cardboard outer sleeve which is marked through storage.
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In the realm of science and technology, ties between diplomacy and business are increasing. The lightning-fast pace of new and evolving technology is driving new lines of business--lines that government policy often has a hard time keeping up with.
"As tech evolves, the public policy landscape needs to change," said Timothy Hughes (F'94), senior vice president and general counsel for Space Exploration Technologies Corporation (SpaceX), a rocket launch services provider which has developed a family of launch vehicles intended to increase the reliability of access to space while reducing costs.
As a student, Hughes always wanted to work in national politics, and after graduating from the School of Foreign Service in 1994, and getting a law degree from The College of William and Mary, he worked first for the Secret Service and then a national law firm, before getting the opportunity he wanted on Capitol Hill.
Following the Space Shuttle Columbia Disaster in 2003, Hughes joined the Committee on Science and Technology in the U.S. House of Representatives in order to focus on the shuttle accident and its causes. He became the primary professional staffer writing the bill that led to The Commercial Space Launch Amendments Act of 2004. This law has governed the way that commercial spaceflight is handled in the U.S. for the past 10 years.
After his work on the 2004 act, Hughes was approached by Elon Musk, billionaire business magnate, engineer and investor, who invited him to join his new rocket company, SpaceX. The company is now the first private entity to ever successfully launch a private spacecraft and to link it with the space station.
SpaceX made headlines last year when it won a NASA contract to carry U.S. astronauts to the International Space Station. They are currently working to be the first entity in history to launch a rocket, bring it back from space, land it and be able to reuse it.
Hughes runs legal and government affairs at SpaceX, making his job both national and international in nature. "Our commercial space launch business has a customer base that is truly international. We negotiate deals overseas, work closely with the federal government and interact with Capitol Hill. I have been able to draw from my experience at SFS in my daily work, because it touches on law, politics business and finance," he said.
As a recent addition to SFS's Board of Visitors, Hughes hopes to be able to use his knowledge and experience in the STEM fields to further the school's mission. He is particularly interested in providing SFS students with greater insight into the link between international affairs and science, technology, and space exploration.
Hughes is part of a longstanding legacy of Hoyas making a difference in the fields of space exploration, law and science. Andrew G. Haley (L'28), known as the world's first space lawyer, co-founded Aerojet Engineering Corp (later Aerojet General), which designed and developed rocket-assisted and jet-assisted take-off units for aircraft during World War II.
Maj. Gen H.D. Polumbo served as a National Defense Fellow at Georgetown from 1999-2000. A decorated officer, he served as Commander of the 9th Air and Space Expeditionary Task Force in Afghanistan and holds the distinction as the first Air Force general officer to fly the U-2S in combat.
Other notable Hoyas in the field include the late Frank A. Taylor (L'34), founding director of the National Museum of American History and director of Air and Space Museum from 1969–1971, and John-David F. Bartoe (M'74, PhD'76), astronaut, astrophysicist and research manager for the International Space Station at NASA's Johnson Space Center.
These Hoya leaders have each had an impact on the field of space exploration, despite taking different paths through foreign service, government and law. The mission of global engagement and the innovative programs of Georgetown will continue to make a mark on this exciting and developing field.


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Allen: I'm devastated but I'll keep trying
"I've just been so close all of the time," a frustrated Sean Allen tells FIFA.com. "I've always been so close, always played online, grinded out the games, always been near the top but just not enough to make it up there."
It's been weeks since the epic finale to the FIFA Interactive World Cup 2016, which took place at Harlem's Apollo Theater, and silver-medalist Sean Allen is still trying to comprehend the sudden and strange shift of momentum in the dying minutes of the second-leg of his gold medal match against Denmark's Mohammad Al-Bacha.
Until that infamous 88th minute, Allen was leading 3-1 and just a few phases away from being crowned the world champion of EA SPORTS™ FIFA 16. Then came a rapid-fire succession of goals, next the final whistle. Final score 3-3 and a victory for his opponent on away goals. Twenty-four-year-old Allen hunched forward in disbelief as 17-year old Mohammad Al-Bacha jumped off the stage, his glasses flying off his face and onto the theater floor as friends surrounded him to celebrate.
"I surprised myself," the Englishman says of his performance in New York. "I surprise myself every day, how I play, how I do that to leads, I just surprise myself all the time."
The phone call Every year, since 2008, Sean has been trying, unsuccessfully, to qualify for the Grand Final. "I always played in the UK live qualifiers," he said. "I always used to lose in the semi-finals, I'd always get so close but never make it."
2016 followed a similar narrative for Allen whose third-place finish on the Xbox Online Season 2 leaderboard (Europe) left him just shy of claiming an elusive Grand Final ticket. Sean's FIWC dreams seemed paused for yet another year. That all changed only seven days before the opening match of the 2016 Grand Final in New York when a phone call informed him of a Grand Finalist's last-minute withdrawal. An unexpected journey ensued from the county town of Taunton in southwest England to the arrivals hall at JFK International.
"I never thought I'd make it there," said the 24-year-old before taking a long pause. "I really just enjoyed playing and being there, even at the end."
The pain of losing the world title in the final moments is clearly still something that weighs on him. Time will undoubtedly soften the blow and Allen will perhaps then be able to enjoy more freely his massive accomplishment - finishing in second place in a field of over 2.4 million registered FIWC players.
One person who intimately understands the early despair felt by the losing finalist is FIWC 2014 runner-up David Bytheway. "No-one wants to ever be the second best player in the world," Bytheway tells FIFA.com. "After I lost in Brazil, for weeks, part of me was always thinking I've done amazing, second in over two million people. But then the other part of me, because I'm so competitive, is thinking I just want to be number one."
Asked what he thought of the final, Bytheway couldn't hold back his frustration. "Sean knows he threw it away. He'll look back on that night and know, those final minutes, if he'd just kept it simple, kicked it up field, into the corners, he would have been the world champion."
Losing a Grand Final in the knockout rounds has pushed some star e-sports players into taking a sabbatical from the game. Allen is defiant though, despite the difficult defeat. "I'm devastated at the end of the day, but definitely I'm going to pick myself up, I'm always going to be trying to qualify for this tournament, I loved every second of my week in New York."

redpajama_set_name: RedPajamaCommonCrawl


This development, located in the Jamaica Plain area of Boston, is part of a revitalization effort in a largely industrial neighborhood through the introduction of residential, commercial and retail uses, as well as green space. The residential portion of the project in which Studio 3.0 is involved creates a new 53,000 square foot, 44 unit building. In addition to cleaning up a currently contaminated site, this development will also bring a public park, community garden, connection to the future Southwest Corridor bike path extension, and significant retail space to the area.
The design of the 44-unit building works to minimize the perceived scale of the project, given its location adjacent to smaller individual triplex homes. This is accomplished by differentiating the building's façade with a series of bays, relating back to the existing residential context. These bays are further articulated with a rhythm of different exterior finishes, allowing the design to suggest individual townhomes, rather than a large singular structure.
This project is currently under construction!


Google Penguin is basically the name of a Google algorithm update that was launched by the company in April 2012. This algorithm was developed in order to control the misuse of SEO techniques by websites. The launch of Google Penguin was seen as a direct attack on websites that indulged in unethical SEO practices for increasing search engine rankings. Some such Black-hat SEO practices which Google Penguin aims to curb include duplicate content submission, keyword stuffing, cloaking, spamdexing, & so on.
While the Google Penguin is all about undermining the efforts of people indulging in questionable SEO tactics, Google Panda targets websites that provide low quality content. Google Panda was all about penalizing websites that offered poor content by decreasing their search engine rankings.
While Google Panda is limited to the USA, Google Penguin is applicable to countries worldwide.
Google Penguin ultimately aims to control spamming & to promote natural links, while Google Panda aims to promote good and useful content on websites. The goal is stop people from indulging in tricks & tactics to lure users to websites with useless/irrelevant content rather focus on providing a good user experience by offering relevant & unique information.
Google Penguin has also had an impact on Article Marketing. There are some things that a website needs to ensure so as to avoid being down-ranked by Google Penguin.
Backlinks - If the Backlinks (all or most) on a webpage are from the same source, the website is sure to be penalized. To avoid this, it is to be ensured that the Backlinks belong to a variety of sources.
Landing Page - Google Penguin also down ranks websites where only a single page is promoted. To avoid this, it is best if websites promote multiple web pages together with Backlinks.
Anchor Text - Google Penguin is all about improving user experience & so it promotes content where there is variety in anchor text. Informative anchor text is also very essential. Informative Anchor Text refers to those anchor text that gives a brief idea about the landing pages where the user is being forwarded.
In the end, Google Penguin emphasizes on improving the overall user experience.


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Glyoxalase 1 gene improves the antistress capacity and reduces the immune inflammatory response
Fukuan Du ORCID: orcid.org/0000-0002-5248-353X1,2 na1,
Yan Li1 na1,
Jing Shen1,2,
Yueshui Zhao1,2,
Parham Jabbarzadeh Kaboli1,2,
Shixin Xiang1,
Xu Wu1,2,
Mingxing Li1,2,
Jiangyao Zhou3,
Yuan Zheng4,
Tao Yi5,
Xiang Li1,
Jing Li6,
Zhangang Xiao1,2 &
Qinglian Wen7
Fish immunity is not only affected by the innate immune pathways but is also triggered by stress. Transport and loading stress can induce oxidative stress and further activate the immune inflammatory response, which cause tissue damage and sudden death. Multiple genes take part in this process and some of these genes play a vital role in regulation of the immune inflammatory response and sudden death. Currently, the key genes regulating the immune inflammatory response and the sudden death caused by stress in Coilia nasus are unknown.
In this study, we studied the effects of the Glo1 gene on stress, antioxidant expression, and immune-mediated apoptosis in C. nasus. The full-length gene is 4356 bp, containing six exons and five introns. Southern blotting indicated that Glo1 is a single-copy gene in the C. nasus genome. We found two single-nucleotide polymorphisms (SNPs) in the Glo1 coding region, which affect the three-dimensional structure of Glo1 protein. An association analysis results revealed that the two SNPs are associated with stress tolerance. Moreover, Glo1 mRNA and protein expression of the heterozygous genotype was significantly higher than that of the homozygous genotype. Na+ and sorbitol also significantly enhanced Glo1 mRNA and protein expression, improved the fish's antioxidant capacity, and reduced the immune inflammatory response, thus sharply reducing the mortality caused by stress.
Glo1 plays a potential role in the stress response, antioxidant capacity, and immune-mediated apoptosis in C. nasus.
The estuarine tapertail anchovy, Coilia nasus, is a commercially important species in China because of its nutritive value and delicacy [1]. The fish is widely distributed in the Yangtze River, the coastal waters of China and Korea, and the Ariake Sound in Japan [2]. C. nasus is an excellent model animal for stress research because it is highly responsive to stress. Transport and loading the fish often induces stress and this stress response can cause sudden death [3], a phenomenon that also occurs in humans [4]. Currently, the key genes regulating stress-induced sudden death in C. nasus are unknown. Therefore, this topic warrants further study.
The glyoxalase system catalyzes the conversion of reactive acyclic α-oxoaldehyde into the corresponding α-hydroxyacid [5, 6]. This system involves two enzymes, glyoxalase 1 (Glo1) and glyoxalase 2 (glo2), and a catalytic amount of reduced glutathione (GSH) [7]. Glo1 is the rate-limiting enzyme in this system and it catalyzes the isomerization of the hemithioacetal that forms spontaneously in the conversion of α-oxoaldehyde and GSH to S-2-hydroxyacylglutathione derivatives, which reduces the steady-state concentrations of physiological α-oxoaldehyde and the associated glycation reactions [8,9,10]. Glo1 reportedly plays an important role in many diseases [11,12,13,14,15], including diabetes, in which this gene is suppressed [16]. Furthermore, Glo1 suppression has also been linked to the development of the vascular complications of diabetes and also to nephropathy, retinopathy, neuropathy, and cardiovascular disease [17,18,19]. Increasing Glo1 activity is important in the treatment of diabetes and these complications [20].
In our previous studies, the stress response in C. nasus induced hyperglycemia, which induced oxidative stress, activated the immune inflammatory response, and caused tissue damage [3, 21]. As we reported, Glo1 alleviated this hyperglycemia-induced damage. Therefore, in this study, we investigated whether Glo1 is associated with sudden death in C. nasus.
Sudden death caused by stress and Glo1 gene response in C. nasus
One hundred eighty individuals from a random population were used in the transport experiment. The results showed that the survival rate decreased sharply at 0–4 h, declined gradually after 4 h, and then became slight after 6 h. Of the initial fish population, 12% were still alive after 8 h (Fig. 1a). These data indicate that individual fish showed different stress tolerance, and this difference arose from genetic differences, which could include DNA variation and epigenetics. However, the genes related to sudden death in C. nasus were unknown.
Transport stress and Glo1 response in C. nasus. (a) Changes in the survival rate after transport stress. (b) Stress response elements (SREs) in the Glo1 genes of different species. (c) Glo1 mRNA expressions in the dead fish and surviving fish groups
Our previous studies have shown that oxidative stress is a major cause of stress damage in C. nasus, so we speculated that the stress-induced sudden death gene/s in this fish should meet two basic conditions: (i) they are involved in oxidative stress; and (ii) they should contain stress response elements (SREs). Based on these two conditions, we identified the Glo1 gene. The sequences of this gene have been reported in humans [22], mammals [23,24,25,26], and fish [27, 28], and its function is conserved. More importantly, SREs have been found in the Glo1 5′ untranslated regions (UTRs) in these species (Fig. 1b). We determined the expression levels of Glo1 in the brains of the surviving and dead C. nasus with RT–qPCR. The expression of Glo1 was significantly higher in the surviving group than in dead group (Fig. 1c). These results indicate that this gene is regulated by stress.
Glo1 gene copies in the C. nasus genome
To clarify the correlation between Glo1 gene expression and sudden death, we determined the full DNA sequence of the Glo1 gene. The full-length gene is 5274 bp long, and contains six exons and five introns (Fig. 2a, GenBank accession number: MK116541). The copy number of Glo1 in the C. nasus genome was determined with Southern blotting, which showed a single insertion site for Glo1 in the C. nasus genome (Fig. 2b).
Schematic diagram of the Glo1 gene structure and Southern blotting results. (a) Schematic diagram of the Glo1 gene structure. The full-length gene is 4356 bp long, with six exons and five introns. (b) Southern blotting results for Glo1 in C. nasus
Association of Glo1 gene alleles with stress
To determine whether the natural variation in any of the Glo1 genes is associated with the variation in stress tolerance in C. nasus individuals, an association analysis was conducted for each SNP in the Glo1 gene. We sequenced the whole gene with six pairs of primers, and two polymorphic loci (495 T/C and 504 G/A) were detected (Fig. 3a), both in the coding sequence.
Association analysis of Glo1 polymorphism and stress tolerance. (a) Two polymorphic loci (495 T/C and 504 G/A) in the Glo1 gene. (b) Analysis of paired-locus linkage disequilibrium of 495 T/C and 506G/A. (c) The mRNA expressions of the heterozygous genotype (TC/GA) and homozygous genotypes (CC/AA, TT/GG). (d) Protein expressions of the heterozygous genotype (TC/GA) and homozygous genotypes (CC/AA, TT/GG)
We tested whether the genotypic frequencies were in Hardy–Weinberg equilibrium using the goodness-of-fit χ2 test. Both P values were > 0.05. A correlation analysis of stress tolerance and the genotype distribution was performed with R3.3.3, and the significance of the correlation was confirmed with the χ2 test. These results indicated that both SNPs were significantly associated with stress tolerance (both P < 0.05; Table 1). For SNP 495 T/C, the CC, TC, and TT genotype frequencies were 17.1, 48.6, and 34.3%, respectively, in the dead group, whereas the corresponding frequencies in the surviving group were 15.8, 78.9, and 5.3%, respectively, which were all significantly different from those in the dead group (all P < 0.05; Table 1). For SNP 506G/A, the AA, GA, and GG genotype frequencies were 34.3, 28.6, and 37.1%, respectively, in the dead group, whereas the corresponding frequencies in the surviving group were 15.8, 78.9, and 5.3%, respectively, which were all significantly different from those in the dead group (all P < 0.05; Table 1). These data indicate that the two SNPs are associated with stress tolerance.
Table 1 Association analysis of SNPs in Glo1 that confers stress tolerance
An analysis of the paired-locus linkage disequilibrium revealed that SNPs 495 T/C and 506G/A were in strong linkage disequilibrium, and they were selected for a haplotype analysis (Fig. 3b). Four common haplotypes were detected in both the dead and surviving groups (global P = 0.152), whereas haplotype TA (495 T–506A) was only found in the dead group (8.70%, P = 0.06).
According to this association analysis, the stress tolerance conferred by different Glo1 genotypes differed. Therefore, the expression of Glo1 could differ in the fish with these genotypes. To test this, we determined the Glo1 mRNA and protein expression in both the dead and surviving fish groups. The results showed the mRNA expression of the heterozygous genotype (TC/GA) was significantly higher than that of the homozygous genotypes (CC/AA, TT/GG) (Fig. 3c). Moreover, the protein expression levels were consistent with the mRNA levels (Fig. 3d). These results indicate that level of Glo1 expression is closely associated with stress tolerance.
By comparing the Glo1 amino acid sequences of different species, we found that the two SNPs are located in conserved regions of the protein (Fig. 4a). Moreover, 495 T/C (126 A:V) and 506G/A (129 D:G) are nonsynonymous mutations (Fig. 4a). Therefore, we predicted the three-dimensional protein structures conferred by the different genotypes, and found that these mutations affected the three-dimensional structure of the Glo1 protein (Fig. 4b, c). This largely explains why these two SNPs are associated with stress tolerance.
Comparison of the Glo1 amino acid sequences and three-dimensional protein structures predicted for the different Glo1 genotypes. (a) Comparison of the Glo1 amino acid sequences of different species. (b) Three-dimensional Glo1 protein structure corresponding to genotype 495 T/506G. (c) Three-dimensional Glo1 protein structure corresponding to genotypes 495C/506A
Glo1 regulation and the stress survival rate
To clarify whether the regulation of Glo1 expression affects the survival rate of C. nasus after stress, the regulation of Glo1 mRNA expression by different ions was investigated with RT-qPCR. We found that a Glo1 agonist, S-ethyl cysteine (SEC), significantly increased the mRNA expression of Glo1 (Fig. 5a). Moreover, seawater salts, Na+, and sorbitol also significantly enhanced Glo1 mRNA expression (Fig. 5a). Among these, Na+ most notably enhanced Glo1 mRNA expression. However, Mg2+ and Ca2+ also significantly inhibited Glo1 mRNA expression (Fig. 5a). The expression of Glo1 protein was detected with western blotting, and the results were consistent with the expression of Glo1 mRNA (Fig. 5b).
Glo1 mRNA and protein expression and the survival rate regulated by different ions. (a) Glo1 mRNA expression regulated by different ions. Data are expressed as the ratio of Glo1 mRNA expression in the brain to its expression in the C2 group (mean ± SD). Data with different superscript letters are significantly different (P < 0.05). C, control, no ions; S, sea salt; C2, control 2, no ions, 8 h after transport; Ga, Glo1 agonist, SEC; Na+, NaCl; Sor, sorbitol; Mg2+, MgCl2; K+, KCl; Ca2+, CaCl2. (b) Glo1 protein expression was regulated by different ions. (c) Changes in survival rate regulated by different ions. (d) Changes in survival rate regulated by different concentrations of NaCl. (e) Glo1 mRNA expression regulated by different concentrations of NaCl. Data are expressed as the ratio of Glo1 mRNA expression in the brain to its expression in the C group (mean ± SD). Data with * are significantly different (P < 0.05). C, no NaCl control; C4, no NaCl control after 4 h stress. The following are the 0.5, 1.0, 1.5, and 2.0% NaCl groups. (f) Glo1 protein expression was regulated by different concentrations of NaCl
Glo1 is associated with sudden stress-associated death, so regulating the expression of Glo1 should affect the survival rate of C. nasus after stress. To test this hypothesis, we injected fish with the Glo1 agonist and found that the 8-h stress-associated survival rate dropped to 58% (Fig. 5c). The addition 0.1% NaCl or sorbitol to the fish culture water increased the survival rate to more than 95%, but the effect of NaCl was most significant (Fig. 5c). To clarify the optimal Na+ concentration that protects C. nasus against stress, we tested five concentrations, and found that 1.0–1.5% NaCl resulted in the highest survival rate (Fig. 5d). These results indicate that NaCl is an ideal antistress agent for C. nasus. Meanwhile, the mRNA expression (Fig. 5e) and protein expression were also significantly upregulated by 1.0–1.5% NaCl.
Changes in Glo1 expression affect immune inflammation and antioxidant capacity
As in our previous study [3], transport and loading stress induced oxidative stress in C. nasus, and this oxidative stress activated the apoptosis pathway mediated by tumor necrosis factor α (TNF-α), ultimately causing tissue damage. Na+ and sorbitol significantly increased the expression of Glo1 and reduced the mortality caused by stress. Therefore, we speculated that Na+ significantly improved the antioxidant capacity and reduced the immune inflammatory response of the fish. To test this hypothesis, we detected the lipid peroxidation (LPO) levels in the fish tissues and intermediates of the apoptosis pathway. The Glo1 agonists, Na+, and sorbitol significantly inhibited the upregulation of all these factors (Table 2). We also detected important indicators of antioxidation, including the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). The Glo1 regulators (SEC, NaCl, and sorbitol) significantly increased the T-AOC and GSH-Px activities, reduced the LPO content, inhibited TNF-α-mediated cellular immune inflammation, and alleviated the injury induced by stress (Table 3).
Table 2 Effects of Glo1-regulating reagents on the apoptosis pathway mediated by TNF-α
Table 3 Effects of Glo1-regulating reagents on total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)
Glo 1 expression in cancers
In order to know if there was a correlation between Glo1 and cancer, first, we compared the levels of expression between normal samples and patients with different kinds of cancers and presented them in a box plot. The results showed that except for GBM, the other cancers all showed significant differences. Most cancers were significantly upregulated in tumor samples, and in contrast, only KIPAN was significantly downregulated. We then conducted a survival analysis of the Glo1 gene in cancers (Fig. 6). Kaplan-Meier analysis showed that about one-half of cancers having differential expression were significantly related to overall survival (OS) (Fig. 7). These results suggested that the Glo1 gene may play a significant role in different kinds of cancers.
RNA sequencing data from TCGA and the GTEX database of cancer patients and normal samples were used to analyze the expression levels of Glo 1 genes in different kinds of cancers. The number of samples is shown in the figure
Kaplan-Meier survival curves of Glo1 genes in different kinds of cancers, based on the expression levels. The orange lines represent high expression and the green lines represent low expression
Stress is a response of organisms to the environment [29, 30]. As previously reported, the stress responses in fish usually activate factors in the hypothalamic–pituitary–adrenal axis [21, 31], releasing the stress hormone cortisol [32], which in turn regulates the metabolism of carbohydrates and lipids [33, 34], and even the immune response. Therefore, fish immunity is not only affected by its innate immune pathways but is also triggered by the stress response. In this study, we investigated the effects of Glo1 gene expression on stress, antioxidation, and immune-mediated apoptosis in C. nasus. Our results show that Glo1 expression can significantly alter immune inflammation and the expression of apoptosis-related factors, in another important mechanism of fish immunoregulation.
Glo1 is reportedly associated with many diseases, including diabetes [35], cancer [36], and depression [37, 38]. However, no association between Glo1 and sudden death has previously been reported. Coilia nasus is a fish with a strong stress response, and its daily management and netting often cause its sudden death [3]. Therefore, this species is an important model for the study of sudden death. In the present study, we found that specific genotypes of Glo1 were associated with sudden death, and that both Na+ and sorbitol significantly increased the mRNA and protein expression of Glo1, which further regulated the fish survival rate after stress. It has been reported that SEC is an agonist of Glo1 [18]. In our study, SEC also significantly increased the expression of Glo1 mRNA and protein in C. nasus. Interestingly, sea salts improved Glo1 expression more significantly than SEC. However, sea salt is a mixture and it is important to know which ingredients in sea salts are most effective. Therefore, we tested Na+, K+, Mg2+, and Ca2+, and found that the effect of Na+ on Glo1 expression was the most pronounced. A gradient experiment showed that Na+ concentrations of 1–1.5% best stimulated Glo1 expression and best reduced the fish mortality rate after stress. We also examined whether the salts themselves or the osmotic pressure they exert regulate Glo1 expression by investigating the regulatory effect of sorbitol on Glo1 expression because sorbitol in water only changes the osmotic pressure and does not affect the ion concentration. Our results showed that sorbitol also significantly increased the expression of Glo1, indicating that osmotic pressure is the key factor regulating Glo1 expression and C. nasus mortality after stress. However, Mg2+ and Ca2+ also inhibited the expression of Glo1.
The occurrence of cancer is closely related to immunity [39]. Because Glo1 can regulate immunity, the gene may be related to cancer. In order to verify this possibility, we analyzed Glo 1 expression in normal and tumor tissues. The results showed that the expressions of Glo1 in most cancer tissues were significantly higher than those in normal tissues (Fig. 6). Then, why is the Glo1 expression level in cancerous tissues significantly increased? According to the Warburg theory [40], tumor cells use the glycolysis pathway to provide energy even under aerobic conditions. At the same time, due to the rapid proliferation of tumor cells, a large amount of energy is required, and carbohydrate catabolism is strengthened; these processes are similar to the stress response. According to our previous research, the glycolysis pathway produces energy and also produces a large amount of reactive oxygen species, which activate oxidative stress [3], and Glo1 plays an important role in the regulation of oxidative stress. Therefore, Glo1 in cancer tissues is at a high level of expression. From this perspective, Glo 1 may be a potential target for cancer therapy.
In summary, in this study, we found that the Glo1 gene is conserved in different species and that SREs occur upstream from these genes. There is a single copy of Glo1 in the C. nasus genome, with two SNPs in the coding region. These cause nonsynonymous mutations that alter the three-dimensional structure of the Glo1 protein. An association analysis showed that the genotypes of the two SNPs correlate significantly with stress tolerance. RT–qPCR and western blotting showed that the expression of heterozygous Glo1 genotypes was significantly higher than the expression of homozygous genotypes. Na+ and sorbitol significantly upregulate Glo1 expression, inhibit immune inflammation, improve the fish's antioxidant capacity, and reduce the mortality caused by stress. Our results collectively indicate that Glo1 is a key functional gene involved in the sudden death induced in C. nasus by stress.
All sample collections were performed in accordance with the Guidelines for Experimental Animals established by the Ministry of Agriculture of China (Beijing, China). The whole study was approved by the Animal Welfare Committee of China Agricultural University (permit number: SYXK 2007–0023).
Experimental animals
C. nasus (average weight, 9.6 ± 1.2 g) were from our breeding base (Qiandaohu, Zhejiang, China). The fish were adapted to a 7.0 × 5.0 × 1.0 m3 aquarium with a water temperature of 24.5 ± 1.0 °C, pH 7.8, and a dissolved oxygen concentration of 9.2 ± 0.5 mg O2/L dechlorinated, aerated water. The fish were fed twice daily, at 07:00 and 17:00.
Stress experiments
The stress experiments were performed as in our previous study [28]. A total of 180 fish were randomly divided into three tanks, each tank containing 60 fish. These tanks were shaken once every 5 min to simulate the transportation process. The death rates were calculated at 0, 2, 4, 6, and 8 h after transport. The mean length of the fish (n = 180) used in this experiment was 230.98 mm ± 9.26 (± standard error of the mean, SEM) and their mass was 70.28 g ± 5.76. These samples were used to analyze the association between Glo1 gene alleles and stress, in a reverse transcription (RT)-quantitative PCR (qPCR) analysis of their Glo1 mRNA expression profiles, and in western blotting, as described below.
Before sampling, the fish were euthanized with 70 mg/L buffered tricaine methanesulfonate (MS-222). The euthanized fish were immediately submerged in crushed ice to retard the degradation of their RNA. Tissue (brain) samples were stored at − 80 °C until later analysis. Total RNA was isolated by RNAiso Plus (Takara, Dalian, China) according to the manufacturer's instructions, and the cDNA was synthesized, and qPCRs performed as described below.
To study the regulatory effects of salt ions on Glo1 expression, seven groups of stress experiments were designed. Sea salt, NaCl, KCl, MgCl2, CaCl2, or sorbitol (1.0% each) was added to the culture water, and each test group contained three replicates, with 30 random fish per replicate. The no-salt group was designated the control group. The stress experiments were performed as described above. The brains of the fish were sampled at 0 and 4 h after transport (as described above), and RT-qPCR analysis of Glo1 mRNA expression profiles were determined, and western blotting was performed as described below.
To study the regulatory effects of different concentrations of NaCl on Glo1 expression, six groups of stress experiments were designed. No NaCl control, 0.5, 1.0, 1.5, and 2.0% NaCl were added to the culture water, and each test group contained three replicates, with 30 random fish per replicate. The no-salt group was designated the control group. The stress experiments were performed as described above. The brains of the fish were sampled at 0 and 4 h after transport (as described above), and RT-qPCR analysis of Glo1 mRNA expression profiles were determined and western blotting was performed as described below.
RT-qPCR analysis of Glo1 mRNA expression profiles
For the Glo1 mRNA expression analysis, total RNAs from five fish in each group were extracted from the brains of C. nasus with RNAiso Plus (Takara, China). The first-strand cDNA was synthesized with the ReverTra Ace® qPCR RT Kit (Toyobo, Osaka, Japan), and RT-qPCR was used to determine the Glo1 expression profiles, using β-actin (actb) as the reference gene. The RT-qPCR primers 40S/40A for Glo1 and B1/B2 for β-actin (Table 4) shared similar melting temperatures (Tm) and were designed to amplify 91-bp and 136-bp fragments, respectively. RT-qPCR was performed on the ABI 7500 Real-Time PCR System (ABI, Foster City, CA, USA) using 2× SYBR green real-time PCR mix (Takara, Japan). PCR amplification was performed in five samples in each group with each sample in triplicate, using the following cycling parameters: 94 °C for 2 min; followed by 40 cycles of 15 s at 94 °C, 15 s at 60 °C, and 45 s at 72 °C. All samples were analyzed in triplicate and the expressions of the target genes were calculated as the relative fold change, using the 2−ΔΔCT method. One-way ANOVA followed by the Bonferroni post hoc test was used to analyze differences among all treatments. A probability (P) value < 0.05 was considered statistically significant.
Table 4 Sequences of primers used in this study
The brain proteins of C. nasus were extracted with the KeyGEN Whole Cell Lysis Assay Kit (KeyGEN, Nanjing, China) and the protein content was determined with a bicinchoninic acid method kit (BCA Protein assay Kit; Pierce, Bonn, Germany). The other procedures were as described in our previous study [28].
Association analysis of Glo1 gene alleles and stress
The whole sequence of the Glo1 gene was amplified with six pairs of gene-specific primers (Table 4). The amplicons ranged from 572 bp to 694 bp in length. Each PCR was performed in an ABI Thermal Cycler (ABI) in a 25 μL reaction volume containing 100 ng of the DNA template. The annealing temperatures were calculated with the Primer Premier 5.0 software (http://www.premierbiosoft.com/primerdesign/index.html). The PCR products from 30 dead fish and 30 surviving fish were purified and sequenced with the ABI 3730 DNA Analyzer (ABI). The polymorphic loci were detected from a sequence alignment of different individuals using Vector NTI Suite 11.0 (Invitrogen, Carlsbad, CA, USA).
To genotype these SNPs with PCR and Sanger sequencing, an additional pair of primers (173S/173A) was designed (Table 4). PCR was performed with all the samples of dead and surviving fish. The resulting fragments were separated on 1.0% agarose gels and purified with the Axygen DNA Gel Extraction Kit (Axygen, Union City, CA, USA). The purified fragments were then sequenced (Tianlin, Wuxi, China).
We estimated the allele and genotype frequencies and analyzed their associations with stress tolerance using R3.3.3 (https://cran.r-project.org/bin/windows/base/old/3.3.3/). To further test the associations between the SNPs and stress tolerance, we tested the linkage disequilibrium based on the genotyping results. The loci in strong linkage disequilibrium were selected for a haplotype analysis with the SHEsis software (http://analysis.bio-x.cn/SHEsisMain.htm). The χ2 test was used to test the significance of differences. P values less than 0.05 were considered statistically significant.
Southern blotting
Southern blotting was used to determine the number of copies of the Glo1 gene in the C. nasus genome, as described previously [41]. Briefly, genomic DNA from the fish brain was isolated with TIANamp Marine Animals DNA kit (Tiangen, Beijing, China), and the DNA concentrations in the samples were adjusted to 100 ng/μL. The genomic DNAs were digested with enzymes BamHI, KpnI, and HindIII. The digested DNA was separated with 0.8% agarose gel electrophoresis. DNA fragments were transferred to a positively charged nylon membrane (Millipore, Boston, MT, USA) and then hybridized with a digoxigenin-labeled Glo1-specific probe (the probe was amplified with PCR and labeled with digoxigenin using the PCR primers Glo1-F/Glo1-R, shown in Table 4). An NBT/BCIP color detection kit (DIG High Prime Lab/Detection K1 kit; Roche, Basel, Germany) was then used to detect the fragments.
Analysis of nucleotide and amino acid sequences
The nucleotide and predicted amino acid sequences of Glo1 were analyzed using the DNA Figures software (http://www.bio-soft.net/sms/index.html). The similarities between Glo1 from C. nasus and the Glo1 genes of other organisms were analyzed using the BLASTP search program (http://www.ncbi.nlm.nih.gov/blast). The C. nasus Glo1 amino acid sequence was compared with those of other species with ClustalX 1.83 (http://www.ebi.ac.uk/clustalW/) and GeneDoc (http://www.nrbsc.org/gfx/genedoc/). A phylogenetic tree was constructed using MEGA 3.1 (http://megasoftware.net; Table 5). The three-dimensional structure of the Glo1 protein was predicted with the SWISS-MODEL online software (https://swissmodel.expasy.org/).
Table 5 GenBank accession numbers of the Glo1 sequences used in this study
Analysis of immune inflammation and antioxidant capacity
TNF-α, cytochrome c, caspase-9, and caspase-3 were analyzed using an enzyme-linked immunosorbent assay kit (Zhaorui, Shanghai, China), as described by the manufacturer. Total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were examined using appropriate detection kits according to the manufacturer's instructions (Nanjing Jiancheng Chemical Industrial, Nanjing, China).
One-way ANOVA followed by the Bonferroni post hoc test was used to analyze differences among all treatments. A probability (P) value < 0.05 was considered statistically significant.
Glo1 expression and survival analysis in cancers
The expression data of GLO1 in pan-cancer were extracted from TCGA database (http://cancergenome.nih.gov) and the GTEX database (https://www.gtexportal.org/home/). Statistical analysis of the differences in expressions were performed using GraphPad Prism 6, with no special comments. Student's t-test was used to compare the difference between two groups. Overall survival was shown as a Kaplan-Meier curve, which was calculated using the log-rank test. A value of p < 0.05 was considered statistically significant. R/Bioconductor survival and the Survminer package were used for survival analyses of GLO1 in pan-cancer.
Glo1:
glyoxalase 1
GSH-Px:
glutathione peroxidase
RT-qPCR:
reverse transcription-quantitative PCR
SNP:
single-nucleotide polymorphisms
SOD:
T-AOC:
total antioxidant
melting temperature
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This work was supported by the National Natural Science Foundation of China (grant no. 31502152) and the National Natural Science Foundation of China (grant no. 31672643). The funders had no role in the study design, data collection, and analysis, decision to publish or preparation of the manuscript.
All the sample collections were performed in accordance with the Guidelines for Experimental Animals established by the Ministry of Agriculture of China (Beijing, China). The whole study was approved by the Animal Welfare Committee of China Agricultural University (permit number: SYXK 2007–0023).
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. The genomic sequence of Glo1 have been uploaded in GenBank (accession number: MK116541).
Conceptualization, ZX; data curation, FD, YL, YZ1(corresponding to Yueshui Zhao), YZ2 (corresponding to Yuan Zheng), TI, XL, and JL; formal analysis, FD and QW; investigation, PK; methodology, YL; data interpretation, JS, SX, XW, ML, TY and JZ; writing the original draft, FD; writing, review, and editing, ZX and QW. All authors have read and approved the final manuscript.
Fukuan Du and Yan Li contributed equally to this work.
Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, China
Fukuan Du
, Yan Li
, Jing Shen
, Yueshui Zhao
, Parham Jabbarzadeh Kaboli
, Shixin Xiang
, Xu Wu
, Mingxing Li
, Xiang Li
& Zhangang Xiao
South Sichuan Institute of Translational Medicine, Luzhou, Sichuan, China
Sichuan Neijiang Medical School, Neijiang, Sichuan, China
Jiangyao Zhou
Neijiang Health and Health Vocational College, Neijiang, Sichuan, China
Yuan Zheng
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
Tao Yi
Department of Oncology and Hematology, Hospital (T.C.M) Affiliated to Southwest Medical University, Luzhou, Sichuan, China
Jing Li
Department of Oncology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China
Qinglian Wen
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Correspondence to Fukuan Du or Zhangang Xiao or Qinglian Wen.
Du, F., Li, Y., Shen, J. et al. Glyoxalase 1 gene improves the antistress capacity and reduces the immune inflammatory response. BMC Genet 20, 95 (2019) doi:10.1186/s12863-019-0795-z
DOI: https://doi.org/10.1186/s12863-019-0795-z
Coilia nasus
Glyoxalase 1 gene
Functional genetics


I'm more an incredibly enthusiastic admirer of all things Shinzi Katoh and of the Japanese 'cute' invasion rather than a die-hard fan. But I will admit to being just a little excited when I crossed paths with the new Australian Kawaii For You website.
It's overflowing with what looks to be the largest selection of Shinzi Katoh items in Australia as well as coveted and hard-to-find Japanese brands Docole, Prime Nakamura and Angoo.
Pick from gorgeous and unique totes perfect for nappy bags or at the pool, tons of Bento and lunch boxes for the littlies to fill any brief (I especially adore the Mon Peluche, Alice in Wonderland and Snow White collections), notebooks, memo pads and letter sets featuring wonderful designs and illustrations and even sippy cups! The letter sets are a fabulous option for older kids to encourage letter writing, fast becoming a lost art.
Kawaii For You will be at the next Brisbane Mathilda's Market on 20 February so don't forget to check out their wares in person if you're in town. If not, everything can be purchased online with prices ranging from $3.50 to $64.95 plus postage.


text:
Compass' smart real estate signs and listings are coming to Waze
The new lighted signs and other listings from the NYC-headquartered brokerage will pop up in the popular navigation app this October
by Veronika Bondarenko
Navigation app Waze has a bright new real estate partnership to show off: Compass just announced that its listings will appear on the app's live map.
"We're taking our mission on the move with the help of Waze," Compass announced in a Facebook post on Friday. "Beginning in October, Compass pins will pop up on Waze's map, indicating listings equipped with our new reimagined sign."
We're taking our mission on the move with the help of Waze. Beginning in October, Compass pins will pop up on Waze's map, indicating listings equipped with our new reimagined sign. Learn more about the latest addition to the Compass technology ecosystem at sign.compass.com.
Posted by Compass on Friday, September 7, 2018
The smart signs, which Compass unveiled in July at Inman Connect San Francisco, feature customizable lighting with a listing agent's name and contact information, and send nearby prospective homebuyers phone notifications about the property. They cost between $500 and $700, according to previous statements by Compass.
As part of the partnership with Waze, the spots where the signs have been placed will, along with other listings, appear as map pins on the GPS app that launched out of Israel in 2006 and now has more than 80 million active users.
Over the last year, Waze has been actively trying to enter the real estate space — through earlier partnerships, its map currently features property ads from Homesnap and open house ads from HomeSpotter.
Compass, which has attracted $808 million in funding and is valued at $1.8 billion, has promoted itself as a different kind of real estate brokerage relying on new and innovative technologies for use by its agents. It's seen rapid expansion in 2018 through a spree of acquisitions and hires.
Email Veronika Bondarenko
Compass launches no-interest loan program to serve agents
Compass to acquire Pacific Union International
Questions raised over Compass' recruiting practices
Are Uber, Waze and Tesla getting into real estate? Not exactly…
CRM Cloze launches new feature to better lead conversion
Marketproof helps real estate data flex its market muscle: Tech Review


text:
Q: Parse.com Cloud Code - Creating new Instance of another object on before save I have two classes, Groups and memberships. When a group is made, I'd like its corresponding membership to be made as well. I attempt to do so like this:
Parse.Cloud.beforeSave("Group", function(request, response) {
    var name = request.object.get("name");

    if (!name) {
        response.error("A Group must have a name");
    } else {
        if (!(/^\w+$/i.test(name))){
            response.error("Only letters and numbers, please.");
        }
        var query = new Parse.Query("Group");
        query.equalTo("name", name);
        query.first({
            success: function(object) {
                if (object) {
                    response.error("A group with this name exists.");
                } else {
                    createMembershipForGroup(request.object, Parse.User);
                    response.success();
                }
            },
            error: function(error) {
                response.error("Could not validate Uniqueness");
            }
        });
    }
});

createMembershipForGroup = function(group, user) {
    var MembershipClass = Parse.Object.extend("Membership");
    var membership = new MembershipClass();

    membership.set("group", group);
    membership.set("user", user);
    membership.save(null,{
      success:function(membership) {
        response.success(membership);
      },
      error:function(error) {
        response.error(error);
      }
    });
}

I receive an error back, saying the following:

E2014-05-06T21:40:10.740Z] v30: before_save triggered for Group for
  user xgzDnZ9h0A   Input:
  {"original":null,"update":{"admin":{"objectId":"xgzDnZ9h0A","className":"_User","__type":"Pointer"},"name":"test","public":false}}
  Result: undefined

Not too sure what the error is. How could I go about debugging code like this so I can isolate the issue easily?

A: Let beforeSave handle only the validation, and move your membership code to afterSave. The problem is probably that you're trying to store a relation to an object that is not yet saved (as you're in beforeSave, the object does not yet have an objectId)

A: problem is you are trying to call an async function and leaves before wait to complete it.
createMembershipForGroup(request.object, Parse.User);//Calling async func
response.success();//Leaves beforeSave function

You should write your createMembershipForGroup function inside success response of your query and return your response.success() method in here
membership.save(null,{
  success:function(membership) {
    response.success(membership); // this should be response of beforeSave
  },
  error:function(error) {
    response.error(error);
  }
});

I will post working code if this not helps.

redpajama_set_name: RedPajamaStackExchange


text:
Top 10 Companies in South Africa 2015
7. Steinhoff
With its headquarters in Johannesburg, South Africa, Steinhoff International is an international integrated retail chain of furniture and fixtures.
It manufactures, sources and retails household goods and furniture. It is positioned to serve specially the value conscious customers of Europe, Africa and the Pacific Rim. Steinhoff basically operates through three business segments which are the African retail Operations, the International retail Operations and the Manufacturing, Sourcing and Logistics of International operations. The International Retail Operations retails furniture, home ware and house hold products. Along with furniture and household products the African Retail Operations also engages in the retail of consumer electronics, office automation, home entertainment and building supplies. Motor vehicles and vehicle servicing parts are also retailed by the African Retail Operations. The last segment i.e. the Manufacturing, Sourcing and Logistics segment offers warehousing and specialized distribution services. Also, Household products and home ware are manufactured as well as imported by this segment.
Steinhoff owns a number of brands in UK as well as in Europe. In the United Kingdom the brands owned by this company include high streets brands such as Harveys furniture, Sleepmaster, Bensons for Beds and Cargo.
The origin of this company traces back to 1964 when its founder, Bruno Ewald Steinhoff, started sourcing furniture from Eastern Europe and selling it in the Western Europe. This later was established as the Steinhoff International Holdings in 1988. In 2011 Conforama was bought by Steinhoff. This Acquisition was a major milestone in the history of the company as Conforama with its distribution network of more than 200 stores across Europe (France, Switzerland, Spain, Luxembourg, Portugal, Croatia and Italy) was the second largest retailer of home furnishings in Europe. In the same year Steinhoff also became a significant shareholder of 30% in the JD group.
Sales (in $ billions): 11.31
Profit (in $ billions): 0.9723
Market capitalization (in $ billions): 22.3


The study reports on the development of a photo therapy tool for educational psychologists within the South African context. A literature study explores the nature, advantages, characteristics and the application of photo therapy with clients in a therapeutic setting. The developmental intervention research design guided the practical development of an electronic photo therapy tool that can be applied by educational psychologists in a therapy setting with clients. The empirical research is qualitative in nature, as the effectiveness of the newly developed photo therapy tool is evaluated by emerging adults and educational psychologists as an intervention strategy. In order to accomplish this purpose, emerging adults, as well as educational psychologists shared their perceptions of the newly developed photo therapy tool. Thus the usefulness of the photo therapy tool was investigated as an intervention approach. Qualitative data generation techniques, focus group interviews and photographs were utilised. The research results show that the newly developed electronic photo therapy tool has the potential, as a modern therapeutic tool, to be used by educational psychologists as an intervention strategy. The developmental intervention research design is a continuous process, and as the life world is forever changing, improvements to the photo therapy tool can always be added according to new suggestions and ideas. The findings allow theorisation about the significance of the photo therapy tool. The study further determines that there is definite potential in the electronically created photo therapy tool, as both the young adults and the educational psychologists perceived the photo therapy tool as a possible and valuable tool in the therapeutic intervention process.


text:
Home Something Special Teacher 'erotically massaged' student, 12, and told her she wanted to marry her
Teacher 'erotically massaged' student, 12, and told her she wanted to marry her
By: TEAM BEPINKU.COM | In: Something Special | Last updated: November 26, 2020 0
A teacher in the United States who erotically massaged a 12-year-old student kissed her and told her she wanted to be her wife and escaped prison.
Former Oakbrook Middle School art teacher Allison Chilton, 27, has been sentenced to five years of probation for her warped relationship with the child.
She pleaded guilty to charges following the 2017 case, when the victim's father found out about Chilton's predatory attempts to seduce his daughter into a sexual relationship.
Chilton, who worked in Summerville, Southern California, took care of the young student and sent her messages including, "I want to be your wife."
In another text she said, "I want to hold you."
According to The Charleston Post and Courier, in addition to the evocative lyrics, Chilton also kissed with his mouth open a number of times and gave "sensual massages" to the 12-year-old.
The victim's father told the detectives that he thought nursing started when Chilton taught his daughter when she was in seventh grade.
He had grown suspicious when he learned that his daughter had had meetings with the teacher in the class when it was empty during recess, Knewz reported.
According to reports, Chilton taught arts and math before she was expelled for her predatory behavior.
Chilton formally resigned from the school district after she was taken into custody in 2018, WCIV reported.
The high school teacher was sentenced to 10 years probation for improper student relationship, but was suspended for five years.
She had admitted charges of criminal incitement to a minor and third-degree criminal sexual conduct with a minor.
In addition, a permanent restraining order was issued for Chilton's underage victim.
The former teacher has also been placed on the sex offender register with active GPS monitoring.
039erotically Courts crime marry massaged039 student Students Teacher Told wanted


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text:
pa marriage license
How to get a Marriage License in PA (in 2023 and beyond)
ByPA Unveiled December 28, 2022 January 1, 2023
Pennsylvania marriage license frequently asked questions (FAQ) The great state of Pennsylvania is a wonderful place to get married. In fact, there were around 75,000 PA weddings in 2022. We have answers to all of your questions. How do you get a marriage license? How much do they cost? What documents do you need? Plus…
Read More How to get a Marriage License in PA (in 2023 and beyond)Continue


text:
Возникновение периодической печати в России относится к годам правления Петра I. Первым регулярным массовым изданием стала газета «Ведомости» («Куранты», писавшиеся вручную в Посольском приказе или Приказе тайных дел со времён Михаила Фёдоровича, составлялись в одном, реже в двух-трёх экземплярах). Первый номер «Ведомостей» был напечатан в конце 1702 года, сделав XVIII век первым веком русской журналистики.

В 1728 году в качестве приложения к «Ведомостям» (ставшим к этому моменту «Санкт-Петербургскими ведомостями») начали выходить «Исторические, генеалогические и географические примечания», первоначально представлявшие собой справочные данные к статьям в газете, но со второго года издания, несмотря на частоту издания (дважды в неделю), приобретшие характер самостоятельного журнала, публикующего независимые от основной газеты материалы. Именно «Примечания» можно считать первым в России журналом. После закрытия «Примечаний» в 1742 году появления следующего журнала на русском языке пришлось ждать до января 1755 года.

Литературно-развлекательные и публицистические издания
 Адская почта (издатель Ф. А. Эмин, 1769)
 Вечерняя заря (редактор И. Г. Шварц, 1782)
 Всякая всячина (редактор Г. В. Козицкий, среди авторов Екатерина II, 1769—1770)
 Городская и деревенская библиотека (1782—1786)
 Детское чтение для сердца и разума (1785—1789)
 Доброе намерение (издатель В. Д. Санковский, 1764)
 Живописец (редактор Н. И. Новиков, 1772—1773)
 Зритель (редактор И. А. Крылов, 1792)
 И то и сё (издатель М. Д. Чулков, 1769)
 Кошелек (редактор Н. И. Новиков, 1774)
 Лекарство от скуки и забот (1786—1787)
 Московский журнал (издатель Н. М. Карамзин, 1791—1792)
 Московское ежемесячное издание (издатель Н. И. Новиков, 1781)
 Невинное упражнение (1763)
 Ни то ни сё (издатель В. Г. Рубан, 1769)
 Поденщина (1769)
 Покоящийся трудолюбец (1784—1785)
 Полезное с приятным (издание Сухопутного шляхетского кадетского корпуса, 1769)
 Полезное увеселение (издатель М. М. Херасков, 1760—1762)
 Почта духов (редактор И. А. Крылов, 1789)
 Праздное время, в пользу употребленное (издание Сухопутного шляхетского кадетского корпуса, 1759—1760)
 Прибавление к Московским ведомостям (1783—1784)
 Пустомеля (редактор Н. И. Новиков, 1770)
 Санкт-Петербургский вестник (1778—1781)
 Санкт-Петербургский журнал (издатель И. П. Пнин, 1798)
 Свободные часы (издатель М. М. Херасков, 1763)
 Смесь (1769)
 Собеседник любителей российского слова (издание Академии наук, 1783—1784)
 Трудолюбивая пчела (издатель А. П. Сумароков, 1759)
 Трутень (редактор Н. И. Новиков, 1769—1770)
 Уединенный пошехонец (1786)
 Утренние часы (1787—1788)
 Утренний свет (редактор Н. И. Новиков, 1777—1780)

Научные и научно-популярные журналы
 Ежемесячные сочинения, к пользе и увеселению служащие (1755—1764)
 Магазин натуральной истории, физики и химии (редактор А. А. Прокопович-Антонский, 1788—1790)
 Месячные исторические, генеалогические и географические Примечания в Ведомостях (редактор Г. Ф. Миллер, 1728-1742)
 Собрание лучших сочинений, к распространению знания и к произведению удовольствия, или смешанная библиотека о разных физических, экономических, також до мануфактур и до коммерции принадлежащих вещах (редактор И. Г. Рейхель, издание Московского университета, 1762)
 Санкт-Петербургские врачебные ведомости (1792—1794)
 Санкт-Петербургские ученые ведомости (редактор Н. И. Новиков, 1777)
 Ученые записки Петербургской академии наук (1728)
 Экономический магазин (редактор А. Т. Болотов, 1780—1789)

Примечания

Ссылки

18
Русская литература XVIII века

redpajama_set_name: RedPajamaWikipedia


It's Thursday evening in Dhaka, the capital of Bangladesh. I am late for an appointment to see my friend Shimanto (lit. boundary [Sanskrit]). On the street I shout 'ei mama jaben?' (Hey uncle, will you go? [Bangla]) to catch an auto-rickshaw (auto [English] man-powered-wheeler [Japanese]). After striking the deal, I sit inside the three-wheeler. As the young driver speeds up almost hitting passers-by and curses 'jyam khub kharap!' (Traffic jam [English] is very bad! [Persian]), I recollect the writing at the back of the car: 'allāḥ sarvaśaktimān' (God [Arabic] almighty [Sanskrit]). After a while, we safely arrive the destination. As we part, I say 'dhanyavāda' (Thank you [Sanskrit]). dekha hobe! (See you! [Bangla]) and the driver returns 'xoda ḥafīẓ!' (God [Persian] be with you! [Arabic]).
Everyday conversation in Bangla is thus rich with linguistic diversity. Bangla is the national language of Bangladesh, which Bangladeshis are very proud of. However, behind the current prestige of Bangla lies a tragic memory of Bangladeshis' struggle for cultural, linguistic, and ethnic freedom.
Bangladeshis symbolically commemorate that struggle every year on February 21st, calling the day Shoheed Divosh (Martyrs' Day) or more commonly, Ekushey (Twenty-first). Ekushey became the cultural symbol of Bangladesh because several sacrificed their lives and hundreds were injured during a march on February 21, 1952, in their effort to promote Bangla as a national language of Pakistan.
As a result of the independence movement that ended the British colonial rule, Dominion of India (current Republic of India) and Dominion of Pakistan (later Islamic Republic of Pakistan) were created in 1947. Pakistan was formed under the leadership of Muhammad Ali Jinnah who led the Muslim League. The nation then consisted of East Pakistan (which later became Bangladesh) and West Pakistan. They were two regionally, ethnically, and linguistically distinct regions. Nevertheless, since the majority of people in these two regions were Muslims the leaders of the Muslim League hoped that their shared religious identity was strong enough to tie them together.
"An auto rickshaw." Photo courtesy of Kiyokazu Okita.
However, maintaining this hope became increasingly challenging as people in East Pakistan quickly realized their economic and cultural differences with West Pakistan. In this context, the status of Bangla in Pakistan became the most explosive political issue. Despite the repeated demand from East Pakistan that Bangla be made one of the state languages alongside Urdu, the most prominent language in West Pakistan, leaders in West Pakistan never acknowledged the demand. In 1948, Ali Jinnah visited East Pakistan and publically announced that only Urdu can be the state language, arguing that among many languages spoken in Pakistan, Urdu best represented Islamic culture and tradition. In the nineteenth century, Urdu became the symbol of Islamic language in South Asia due to its use of Arabic letters and its shared vocabulary with Arabic and Persian. Moreover, Bangla was typically associated with Hindu culture due to its connection with Sanskrit.
In January 1952, the Muslim League finally made Urdu the official state language of entire Pakistan. Political leaders, the representative of cultural organizations, and students in East Pakistan immediately expressed their opposition, leading to a demonstration on February 21st. In the demonstration, several people died and many were injured as they clashed with the police.
In the following years, Shaheed Minar (Martyrs' Column) was erected and the celebrated song, Amar Bhaiyer Rakte Raṅgano (Colored in the blood of my brother), was composed. The collective memory of this tragic event fostered the development of the regional identity distinct from that of West Pakistan, which eventually led to the independence of East Pakistan as People's Republic of Bangladesh in 1971. The language of Bangla thus became a symbol of Bangladeshis' cultural, regional and ethnic identity.
After independence, the memory associated with Ekushey increased. Nowadays, Bangladesh dedicates the entire month of February to celebrate the Bangla language. A large book fair is organized in Dhaka and various cultural performances are conducted throughout the country. The significance of Ekushey further became international when, in 1999, UNESCO declared February 21st as International Mother Language Day in order to celebrate language diversity worldwide.
It is certainly important to learn one's mother tongue because language is the vehicle of culture and tradition. Thus older generations' complaints about young generation's Hinglish and Banglish is well justified. That said, since language and nationalism are closely related, we should be careful that emphasis on mother tongue not lead to a narrow-minded linguistic nationalism either. Unfortunately, however, this is what happened in post-independent Bangladesh.
Sheikh Mujibur Rahman, the first Prime Minister of Bangladesh, sought to build the new nation on the principles of nationalism, secularism, democracy, and socialism. In his effort to unite the country, he promoted Bangla as the national language of Bangladesh. However, while Bangla is the language spoken by the majority of people in Bangladesh, there are minority communities such as Manipuris, Chakmas, and Santals whose mother tongues are not Bangla. Nevertheless, Mujibur asked them to embrace Bangla, rejecting their right to maintain their mother tongues. This is sad as it undermines the very principle of Ekushey, through which Bangladesh claimed its independence from Pakistan.
When those who are dominant – due to their strength or numbers – force the other to adopt their language, as West Pakistan did to East Pakistan, or Mujibur to minorities in Bangladesh, it becomes a form of violence. However, pride in mother tongue does not have to lead to narrow-minded sense of superiority in one's ethnicity or nation. As in the case of Bangla, we can observe in any language the influence from other languages. On International Mother Language Day, perhaps we can reflect on the linguistic diversity within our mother tongue that reflects a rich history of interaction.
Image Credit: "Shaheed Minar on Ekushey, 21st February." Photo courtesy of Kiyokazu Okita.
Kiyokazu Okita is an Assistant Professor at The Hakubi Center for Advanced Research and Department of Indological Studies, Kyoto University. He is the author of Hindu Theology in Early Modern South Asia.
I hope that Esperanto was not forgotten today. Esperanto is a planned language which belongs to no one country or group of states. Using it brings speakers of different mother tongues together without having to resort to English or a strong regional language.
How do we translate 'International Mother Language Day' in other languages?
It is interesting that someone mentioned Esperanto here, since Sanskrit was similarly an artificially refined lingua franca. It enabled for centuries learned pandits who spoke different Indian dialects as their mother tongues to communicate and share an immense body of literature larger than those of other cultures combined.
The modern-day situation of Hindi is different as Okita pointed out. English and other national languages (e.g. Putonghua) threaten other minority languages into extinction.
My experience in Esperanto is very positive and critics of artificial languages most often have no experience in them. As a native speaker as a minority language (Cantonese), I have to struggle with others' languages all my life. I became fluent in Esperanto a year or so after I learned it for a few weeks and I have been speaking it for almost twenty years since. It is not surprising that most of my Esperanto friends from around the world are also native speakers of minority languages.
"Internacia gepatrolingva tago" what includes also the father.
In Telugu, 'International Mother Language Day' translates to 'Antarjateeya Maatru Bhashaa Dinotsavam'.


text:
Freshwater levels and flows (1)
Lake/River ice (10)
Sea temperature (2)
Snow depth (1)
Snow melt (1)
Show less Show less Variables
Lake/River ice
BC Water Resources Atlas (Opens in a new Window)
The BC Water Resources Atlas is an iMapBC application with enhanced functionality to display detailed water related data, including watersheds, water quantity and quality monitoring sites, aquifers, water wells and flood protection works. This tool presents raster data for a variety of layers.
Government of British Columbia
Ministry of Environment and Climate Change Strategy
Climate Indices (Opens in a new Window)
Climate Indices includes time series, seasonal, and annual data for a number of variables including sea & air temperature, salinity, runoff, flow, ice coverage, iceberg count, and North Atlantic Oscillation Index. Data are observed or observation-based, and are available in a variety of temporal resolutions and historical periods, depending on the variable of interest. Data are available in tabular and graphical formats.
Marine Environmental Data Service
FTP Yukon Data (Opens in a new Window)
The File Transfer Protocol (FTP) Yukon Data directory provides access to a wide range of Yukon datasets. Observational data includes biological and biophysical data, culture and heritage information, elevation, environmental monitoring, forestry and geologic data, imagery, planning, and data from the natural resource sectors. Historical data are available as point and gridded data.
Government of Yukon
Ice climatology (Opens in a new Window)
The Ice Climatology dataset contains long-term information on ice conditions over specific geographic regions. It can be used to examine annual average conditions as well as minimum and maximum ice extent. The dataset includes seasonal summaries, annual Arctic ice atlases, 30-year ice atlases, and ice coverage records. Specific measures include average ice concentration, ice type, frequency of sea ice, old ice concentration, and freeze-up/break-up dates.
Ice Graph (Opens in a new Window)
IceGraph 2.0 generates ice coverage bar graphs and data tables for a selected region and date range. Data are available from 1968 to present and can be compared between seasons. Specific measures include first year ice, new ice, young ice, and old ice for the current season as well as for the historical monitoring period.
Ice Thickness Program Collection, 1947-2002 (Opens in a new Window)
Ice Thickness Program Collection, 1947-2002, contains ice thickness and snow depth measurements for 195 sites across Canada. It includes two historical datasets of point data dated from 1947-2002 and 2002-2016. The record length varies from site to site and many sites had stopped recording by 2002 but measurements were reinitiated in the fall of 2002. Several sites are co-located with Environment and Climate Change Canada's weather and radiation stations.
Icepac interface (Opens in a new Window)
Icepac project aims to develop a probability model of the space-time variability of sea ice at the local scale based. The descriptive and statistical tools available under IcePAC provide users with information on ice conditions and allow them to better understand the temporal and spatial evolution of sea ice. This information can help users to protect against risks to ships and maritime infrastructure.
Government of Canada: Natural Resources Canada, Ouranos, Institut national de la recherche scientifique
NatureWatch (Opens in a new Window)
NatureWatch hosts several nature monitoring programs, with more to come in the future. Many community driven programs record observations of various climate variables and species. Registration is required to download data.


text:
Lana, fashion blogger (Yo quisiera) è una telenovela spagnola per ragazzi, prodotta da Claro e Mediaset España e trasmessa dal 9 novembre 2015 su Divinity.

Il 4 gennaio 2016 è stata confermata la produzione di una seconda stagione che va in onda nel 2018.

In Italia, la prima stagione è trasmessa dal 18 aprile 2016 su La5. La seconda stagione è ancora inedita.

Trama
Lana è una ragazza di 16 anni amante della moda. Attraverso il blog 'Yo Quisiera' riesce a diventare famosa, pur mantenendo la propria identità anonima.

Episodi

Personaggi e interpreti
 Lana Madero, interpretata da Lucía Gil.
È una ragazza sedicenne molto intelligente, gestisce il blog "Yo Quisiera" in anonimo ed ama cantare. Ha due migliori amiche Mara e Julieta. Si innamora di André un ragazzo più grande di 5 anni.
 André Vegas, interpretato da Christian Sánchez.
È il barista del bar della scuola di Lana, h 21 anni, è un cantante fantastico, si farà aiutare da Lana che gli farà da comunity manager. Suo padre è un regista ma non è in buoni rapporti con lui. All'inizio ha una ragazza Patricia ma dopo la lascerà perché pensa che lo stia usando.
 Maria, interpretata da Natalia Rodríguez.
È la migliore amica di Lana. Molto vivace e solare. All'inizio si metterà con Nico ma a causa delle differenze tra i due si lasceranno, si metterà co Ricky, il migliore amico di Nico.
 Julieta, interpretata da Barbara Sínger.
È un'altra delle migliori amiche di Lana. Verso fine di stagione si metterà con Nico e litigherà con Maria.
 Camila, interpretata da Renata Notni.
La bulla della scuola, è una elle ragazze che segue il blog di Lana ma a scuola la sminuisce sempre. La sua migliore amica è Natalia con la quale litigherà ma faranno pace.
 Natalia Sofía, interpretata da Andrea de Pablos.
La migliore amica di Camilla, è molto vivace e litiga molto con il fratellino. Quando litigherà con Camilla si avvicinerà a Elena; ha una cotta per Pablo, il migliore amico di Diego.
 Diego Madero, interpretato da Jaime Olías.
Il fratello di Lana alla quale vuole molto bene, è appassionato di videogame. Ha un migliore amico, Pablo, con il quale passa molto tempo. Piano piano comincerà ad innamorarsi di Elena.
 Pablo, interpretato da Martín Barba.
Migliore amico di Diego, ha una cotta per Lana.
 David, interpretato da Joel Bosqued.
Il bullo della scuola, ed è figlio del preside della scuola, è molto fastidioso e rende la vita impossibile a Gustavo. Ha una cotta per Camilla
 Elena, interpretata da Maria Hinojosa.
La "secchiona" della scuola. Farà un cambio di look quando Camilla avrà bisogno di lei. Ha una cotta per Diego.
 Nicolás "Nico", interpretato da Mikel Iglesias.
È un amico di Lana, si metterà con Maria ma dopo la lascerà mettendosi con Julieta.
 Ricky, interpretato da Gerard Martí.
È il migliore amico di Nico, aiutando l'amico con Maria si innamorerà di lei.
 Patricia, interpretata da Carla Medina
È la ragazza di André, pensa di essere brava a recitare ma non è così; Andrè la lascerà perché pensa che lo stia usando.
 Claudia, interpretata da Isabel Burr.
La psicolaga della scuola, il suo ragazzo Miguel la tradisce ma lei la perdona. Si avvicinerà molto a Gustavo.
 Gustavo, interpretato da Rafael Reaño.
Il bidello della scuola, si innamora di Claudia.
 Nestor, interpretato da Pablo Raya.
È il proprietario del locale "Microfono Aperto"
 Lope, interpretato da Víctor Palmero.
È un vecchio amico di Nestor che è innamorato di lui.
 Miguel, interpretato da Rodrígo Cuevas.
Il ragazzo di Claudia.
 Raúl, interpretato da Santi Díaz.
Il fratellino di Natalia, appassionato di alieni.
 Jorge, interpretato da Fernando Gil.
Il papà di Lana che compare solo quando ha bisogno di soldi.
 Víctor Vegas, interpretato da Alberto San Juan.
Il padre di André.
 Isabel Madero Montesinos, interpretata da Natalia Millán.
La mamma di Lana.

Note

Collegamenti esterni


text:
global harvests Banana & Cacao, Rice, Potato
Artist John Dyer is recognised as one of the UK's leading ethnobotanical painters. He has been appointed as the artist in residence by the International Rice Research Institute in the Philippines, Bioversity International in Costa Rica and the International Center for the Potato in Peru.
Peru - the United Nations Year of the Potato
A 46 minute video diary by John Dyer shot in Peru
John Dyer painting for the United Nations Year of rice in the Philippines
'Global Gardens' painting project with John Dyer & Kim Wilde
Over 500 school children engaged with John Dyer's ethnobotanical art work through the 'Global Gardens' project which the artist ran with Rock Star Kim wilde, the National Trust, Gyllyngdune Garden and Falmouth Art Gallery.
View the children's art now
"I have always had immense respect for anyone who chooses to take time to interpret our world for us through painting, and feel especially privileged to have hosted such an inspired artist in our back garden!"
Rock Star and Gardener
"John Dyer is one of Britain's leading and best known contemporary painters. Born in 1968 his work embraces colour and the 'Song of Life'."
"IRRI had the pleasure of hosting John Dyer as our artist in residence for the United Nations' International Year of Rice. John produced a series of paintings that interpreted the relationships among people, the rice plant and the rice ecosystem.."
Duncan Macintosh
Development Director for the International Rice Research Institute, Philippines: 2004
"We asked John Dyer to paint bananas in Costa Rica because they are a smallholder crop – grown in people's back gardens and farms along with lots of other fruits and vegetables. John's pictures are all about diversity and bringing out what might pass unseen. In this case, it seems to have been scorpions!"
Charlotte lusty
International Network for the Improvement of Banana and Plantain: 2003
Our Supporters and Partners
John Dyer's Global Gardens Project was kindly supported by Falmouth Art Gallery, The National Trust, Rock Star Kim Wilde, CGIAR, Gyllyngdune Gardens, Heritage lottery Fund


The official name is Carlos n' Carmelo's but locally I've not heard anyone call it anything except Carmelo's. When I walked in the first thing I noticed was the nice air-con they had going. The whole restaurant looked very clean and the 2 workers I encountered were very friendly and helpful. I have eaten at this restaurant and I enjoyed it. I actually ate some beef here and it wasn't so bad, and I don't eat beef hardly at all here. Be sure to read the comments at the bottom of this post to get mine and anyone else that has eaten at this restaurant opinion of the food and service.
From UJ: The atmosphere at the restaurant was nice. The wall unit air conditioner was running and it was nice and cool inside. There are no menus for the tables and the waitress does not come to the table, so you have to order up front, then be seated where you want. They will bring the food out to you. All that Lita ordered was the halo-halo (P90). I ordered the cheese sticks, 2 orders of 3 each at P70 each order (P140). I also ordered 2 tacos, 1 double order at P90 (a single taco is P55). For my final food order I asked for 1 order of lasagna (P45). To drink I ordered 1 large iced tea (P30) because they told me they used mineral water to make the tea. Just so you'll know, I don't think many places here has just regular iced tea. I'm pretty sure that at least 90% of the places that have iced tea, have lemon flavored tea, and most of that is Lipton Iced Tea.
The tea was good, so good in fact I ordered a second glass, for which you do have to pay, no free refills. Surprisingly to me the lasagna was good. I had no problem with the beef they used, which was my main concern. The tacos were also good, except that they put mayonnaise on them. Next time, no mayo. The cheese sticks were excellent, the best thing out of everything we ordered. They were about 50% bigger than the ones I'm use to getting in Texas (that's hard for a Texan to admit). Also surprisingly the thing that was liked least about the order was the halo-halo. It was not exactly what Lita expected and she said she will not order that again, there.
Our entire order, including the extra iced tea, was P425 (approximately $10). We were very satisfied and plan to visit there again. Lita really wants to get some more of those cheese sticks.