text:
'Where was the planning?': James City County Supervisors critical of COVID-19 vaccination process during health department update
Top 10 breakfast foods
Hampton to build a new $7.5 million Wythe fire station near baseball field
Opinion: Virginia should seize this moment to abolish the death penalty
Some students to return to York classrooms Monday
Lawmaker looks to rein in law enforcement's use of facial recognition technology
Wednesday's regional sports roundup: CNU women reach Capital finals in field hockey, soccer; Apprentice ranked first in wrestling poll; Admirals swamp Rabbits to go to 5-2
Daily Press |
COLLEGES:
Field hockey:
Courtney Fiest scored the winning goal in the 65th minute as third-seeded Christopher Newport (13-5) came from behind to win 2-1 Wednesday at second-seeded York (Pa.) and reach the Capital Athletic Conference tournament final.
The Captains, ranked 16th nationally, advanced to play at top-seeded Salisbury at 6 p.m. Saturday. The Sea Gulls are No. 7 in the nation.
The York Spartans' season ended at 10-8.
Greta Plappert's goal in the 24th minute put York ahead, but Jackie Kotoriy tipped in the tying goal on a Fiest shot in the 31st. Fiest, a Tabb High alumna, has registered a goal in her last four postseason games. ...
Old Dominion juniors Rebecca Birch and Erin Huffman were named to the All-Big East first team.
Birch led ODU in goals (10) and scoring (23 points) from her center back position and helped the Monarchs post five shutouts.
[Top stories] A man drove to Virginia Beach to kill his wife. He ended up paralyzed and sentenced to 15 years in prison. »
Huffman made the first team for the second consecutive season. The Pennsylvania native ranked second on ODU in points with 22, on nine goals and four assists. Huffman scored a league-best five game-winning goals. …
Virginia senior midfielder Greta Ell made the All-Atlantic Coast Conference second team.
North Carolina, the nation's top-ranked team, swept the major awards. Erin Matson was the Offensive Player of the Year and Freshman of the Year, Ashley Hoffman was the Defensive Player of the Year, and Karen Shelton was the Coach of the Year.
Women's soccer:
Christopher Newport (16-1) stayed perfect against CAC opposition, winning 3-0 at home against fifth-seeded Salisbury in a tournament semifinal.
[Top stories] Hampton's annual MLK Jr. commemoration will honor Rosa Parks, Harriet Tubman and local heroes — virtually »
The Captains, ranked 11th by d3soccer.com, will be at home for the championship game against second-seeded Mary Washington. The Sea Gulls' season ended at 7-10-1. ...
Hosting all seven games of the Conference USA tournament, fifth-seeded Old Dominion saw a two-goal second-half lead slip away in a 3-2 overtime loss to fourth-seeded Louisiana Tech, ending the Monarchs' season at 8-7-4.
Goals by Raegan Tate on a header in the 42nd minute and Amanda Nhek on a low kick in the 48th, both assisted by Kyra Ricks' crossing passes, put ODU ahead 2-0. But the Techsters replied with goals in the 55th, 86th and 93rd — in the third minute of extra time.
Elizabeth Doll netted Tech's first goal with a header off a long free kick. Hanna Banks tied the game, forcing overtime. Early in the extra period, Mylene Roy-Ouellet scored her seventh goal of the season off a pass from Connor Barker.
Friday, Louisiana Tech will face top-seeded North Texas. The Mean Green shut out No. 8 Rice 2-0. Madeline Guderian scored off a corner kick in the 64th minute and assisted an insurance goal by Carissa Sanders in the 74th.
[Top stories] Civilian 'ride-along' shot in 2019 ambush files claim against Newport News Police and city »
In the bracket's other half, No. 6 UAB reached the semifinals for the first time since 1999, ousting No. 3 Southern Mississippi 1-0 on Miah Zuazua's goal in the 78th minute.
At 4:30 Friday, the Blazers will face No. 2 Florida Atlantic, which defeated No. 7 Middle Tennessee State 2-1 when Emma Grissom scored three minutes into the first overtime period.
Amber Hoot gave MTSU the lead 40 minutes into the game, but FAU's Tiril Haga netted the equalizer in the 60th minute.
Men's soccer:
Seventh-seeded Virginia Tech (10-5-3) defeated Syracuse 3-1 in the Atlantic Coast Conference tournament's first round on goals by David Sanz, Kristo Strickler and Nico Quashie in Blacksburg.
[Top stories] Virginia Beach man charged with entering U.S. Capitol during riots »
The Hokies, ninth in the national RPI, will travel for Sunday's quarterfinal against No. 2 North Carolina and seemingly have an NCAA tournament bid in hand. ...
Pittsburgh (8-10) upended sixth-seeded Virginia 2-0 in the ACC first round in Charlottesville, though the Cavaliers (9-3-3) — fourth in the RPI — are a lock to qualify for the NCAA tournament. Edward Kizza scored in each half, giving him an ACC-leading 15 goals for the year. ...
VCU (10-5-1, 6-1-1) took the Atlantic 10 regular-season championship by coming from behind to beat St. Bonaventure 2-1 at Sports Backers Stadium.
The Rams, who will play an A-10 quarterfinal at home at 2 p.m. Sunday against eighth-seeded Massachusetts, scored both of their goals in the second half.
[Top stories] Former Portsmouth Police Chief Angela Greene has told the city she plans to sue »
Entering the final three weekends of the regular season, James Madison was ranked No. 2 by the NCAA Football Championship Subdivision selection committee, as the group unveiled its current top-10 ranking Wednesday night on ESPN2.
This is the committee's only in-season ranking it will release ahead of Selection Sunday, which takes place on Nov. 18 at 12:30 p.m. on ESPNU.
The Dukes are 6-2, including a 4-1 mark in Colonial Athletic Association play.
JMU ranks in the top five nationally in nine statistical categories, which includes leading the FCS in punt-return average (25.63). The Dukes are also second in both defensive touchdowns (4) and fewest penalties (3.75), third in scoring defense (9.8), completion percentage (68.7), first-down defense (107), fourth in third-down defense (25.9 percent) and fifth in both total defense (259.1) and fewest penalty yards (34.13).
The Dukes were one of three CAA schools to be ranked in the top 10, as they were joined by No. 5 Elon and No. 10 Delaware.
Defending champion North Dakota State (7-0) leads the field. Following JMU are No. 3 UC Davis (7-1), No. 4 Weber State (6-2) and No. 5 Elon (5-2).
Coming in at No. 6 is Kennesaw State (7-1), followed by No. 7 South Dakota State (5-2), No. 8 Wofford (6-2), No. 9 Eastern Washington (6-2) and No. 10 Delaware (6-2). …
The CAA released its schedule for the 2019 season, a slate that will be played over 13 weeks for the first time since 2014.
With the additional week of competition, six CAA schools are currently scheduled to play 12 games and six schools are slated to maintain an 11-game schedule with two bye weeks.
Aug. 31 will mark the first games for the CAA's three Virginia members. William and Mary will play host to Lafayette (Pa.), JMU will visit West Virginia, and Richmond will be at home against Jacksonville.
Wrestling:
Apprentice was ranked first in the National Collegiate Wrestling Association preseason poll, followed by state rival Liberty.
The NCWA encompasses schools of all sizes that don't have NCAA wrestling teams. The Builders were the runners-up nationally a year ago.
Many Builders earned preseason weight-class rankings.
Colton Messick, a defending national champ, is ranked first at 125 pounds, while teammate Anthony Monahan is fourth at 125.
At 133, Kolin Johnson is second while freshman Matthew Farrar is sixth.
Shalek Colon (141) and Ty Boyd (149) are third. At 157, Bryson Woody is sixth.
At 165, Chad Simmons is ranked first. At 174, Dan Henson and Jeremy Mosely are ranked sixth and 12th, respectively.
At 184, Wes Sorey is 10th. At 235, Stephen Francisco is fifth. At 285, Peter Cortapasso is fourth. ...
Old Dominion was picked to finish second among the Mid-American Conference's eight teams.
Missouri was named the favorite with 27 points, while ODU had 17.5, Ohio 15.5, Kent State 12, Central Michigan nine, Buffalo and Southern Illinois-Edwardsville eight each and Northern Illinois three.
ODU sophomore Michael McGee (125 pounds) and junior Larry Early (157) were ranked first in the MAC in their weight classes. Junior Sa'Derian Perry (141) and senior Seldon Wright (165) were listed second, junior Shane Jones (174) third, junior Kenan Carter (149) fourth and junior Antonio Agee (184) tied for fourth.
PRO HOCKEY:
William & Mary women overcome Drexel, emerge even in series
Bryant, Norfolk State men survive in overtime against winless Delaware State to go to 3-1 in MEAC
USC Upstate women defeat Hampton to gain split of two-game Big South series
Tyrese Jenkins' 21 points spark Norfolk State men to series-opening victory at Delaware State
U.Va. breakdown: Virginia races out to huge lead against ice-cold Clemson, cruises to largest margin of victory ever against ranked foe
The Norfolk Admirals (5-2) won 3-2 over the Greenville Swamp Rabbits (2-5-1-0) at Scope Arena in their first of eight ECHL meetings this season.
Domenic Alberga scored a goal and added an assist for the Admirals.
Matt McMorrow's goal for Norfolk 9:12 into the second period tied the game at 2, and Connor Hurley's 16:12 into the middle period stood as the winner.
Goalie Ty Reichenbach made 28 saves for the victory.
The Admirals next will face the Atlanta Gladiators in Georgia on Friday and Sunday.
Norfolk Admirals
Manchester Monarchs
Salisbury Sea Gulls
Hampton University


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Stanford conveys digital learning opportunities on multiple online platforms, including Lagunita (an instance of the OpenEdX platform), Coursera, and NovoEd. Data collection infrastructure developed by CAROL converts platform exports into tabular formats that are amenable to analysis with many statistical software packages. More information on available data can be found here.
CAROL makes data from Stanford's online courses available to qualified Stanford investigators. We also share limited data collected from Stanford's public courses conveyed on Lagunita after June 14, 2014 with qualified investigators at other institutions. Applications for data may be submitted here.


Like many of you, I'm a mom with a million things on my plate and ALL the irons in the fire. I homeschool three kids, I work part time, I juggle my daily passion of writing and my first love in my sweet husband of 13 years. Right now, it's ten years into my journey with an anxiety disorder and things are much improved, but stress is still my constant companion. Let's face it, even if you don't suffer from clinical anxiety*, there are plenty of things to be overwhelmed about. I've come to accept that for me, life=anxiety and I just have to figure out the best way to combat it with lifestyle tweaks and changes. Here are 7 small life tweaks that are saving my sanity with instant payoffs!
Become an Early Bird: One of my favorite habits that I live by is getting ahead of the clock each day. It's taken a lot of stress off of me and helps me to feel like I am aggressively taking on life's challenges rather than being constantly attacked by my circumstances. Read about my early arrival habit and how easy it is to implement. I promise, it can change your life!
Simplify your Wardrobe: Simplifying your daily wardrobe doesn't have to involve color-coded spreadsheets, algorithms or appointments with personal shoppers. It can be as simple as choosing your 10 favorite outfits and simply rotating them for a trial month. You will cut back on "decision fatigue", ease the laundry burden and get more comfortable in your own skin. For me, the more I have simplified the less I think about my clothing at all, which is a huge relief! If you want to take this a step further and create a capsule wardrobe, check out my tips here.
Increase your Water Intake: Hydration doesn't have to be complicated and we all know it's vital for health and well-being. What's the easiest way to up your water intake? Get the biggest possible cup you can find, fill it with water and carry it everywhere. If you go with an insulated mug like the RTIC 30 oz Tumbler, your water will stay cold and fresh for-EVER! Throw in a lemon slice for an extra zap of vitamin C and flavor. I don't really have to share the benefits of hydration, do I?
Clearing Kitchen Surfaces. I never thought I would be one to have empty counter tops but I am making my way there little by little and the difference has been so satisfying! How long does it really take to remove and replace an appliance from a cabinet? Truthfully, only half a minute or so. If you keep the majority of your appliances stored behind the scenes you will be gaining a TON of space and make cleaning so much faster (wiping down becomes a 2 second task instead of a complicated 3D obstacle course). You will also be saving yourself the work of dusting or cleaning off grease spatters that happen when appliances are too close to the food-prep action.
Create a daily bag routine. This may be counter-cultural, but hear me out. At some point I realized that my nicer totes and bags were causing chiropractic issues and around the same time I was starting out on my minimalist journey. I decided to take the opportunity to eliminate all the bags and use ONLY ONE for all my needs. (I did keep a dressy bag for rare formal events). I picked a simple black crossbody bag, but the style really doesn't matter. The point is, you have one bag so you save yourself the trouble of switching bags, losing receipts, and failing to have your sunglasses or painkillers right when you need them. I have only four things in my bag: my cell phone, my wallet, my keys and a zipper bag. (In the zipper bag, I keep all my daily essentials such as: painkillers, sunglasses, hand-sanitizer, lotion, lip balm and feminine supplies.) It's easy to keep up with one bag and to keep it free of stray trash and receipts. I hang my purse right by my door as soon as I walk in and it is ready to go for the next outing! I love the simplicity and the time saved and I would never go back to large collection of handbags.
Set One Dinner in Stone. If you are already an expert at meal-planning then you are probably well past this concept. However, if you find meal-planning quite daunting and feel like you are always throwing food on the table at the last minute, I encourage you to start simple. Choose one evening a week to be an easy, healthy, family-friendly recipe and stick with the plan. This could be very specific, like Taco Tuesdays or more general like planning meatless Mondays and rotating through different vegetarian recipes. If you have that one night anchored in your mind, you will more easily be able to make shopping and cooking habitual and routine; again, fighting the dreaded decision fatigue. Take meal-planning a step further with this awesome magnetic weekly menu!
Wake up 15 minutes earlier. Ok, so I have saved the "hardest" for last, but I am certain you will find this tip invaluable if you give it a chance! I believe I first heard about this suggestion on the Purposeful Home Podcast (which I highly recommend). The suggestion is geared towards moms who are normally woken by their early-rising offspring (that's 99% of the moms I know). Instead of letting the children set the tone for your day, you take charge by choosing to get up ahead of time. That way you can start the day off on the right foot, greet your kids in a wakeful rather than zombie state and attack your morning routine intentionally rather than haphazardly. I love the difference when I follow this simple tip!
I told you that these would not be earth-shattering or complicated, but small changes can truly make a big difference in the quality of your life. I love this quote by Nathan W. Morris: "Edit your life frequently and ruthlessly. It's your masterpiece after all." Abandoning my fear of change and focusing on small steps in the right direction has helped me immensely and I know it can help you too! Let me know in the comments what simple life hacks that have made all the difference for you!
*If you are one of the 40 million people in the US who are affected by depression or anxiety, you can find this daily stress to become nearly debilitating. If you suspect you might have a clinical anxiety issue, I encourage you to talk to your doctor or a mental health professional. No life hack can take the place of quality health services!
Up to 50% Puma shoes & clothing!
Waking up early is the best way to kick start your day.


text:
Thiopyrylium je kation se vzorcem C5H5S+, analog pyryliového kationtu, ve kterém je atom kyslíku nahrazen sírou.

Soli tohoto iontu jsou méně reaktivní něž příslušné pyryliové soli, protože má atom síry vyšší polarizovatelnost.

Z chalkogenových šestičlenných nenasycených heterocyklů má thiopyrylium nejvyšší aromaticitu, protože má síra podobnou elektronegativitu jako uhlík a jen o málo větší kovalentní poloměr.

S vodou thiopyrylový ion reaguje za vzniku směsi 2-hydroxythiopyranu a 4-hydroxythiopyranu.

Thiopyryliové soli lze připravit odštěpením vodíku z thiopyranu pomocí akceptoru hydridových iontů, jako je například tritylperchlorát.

Thiopyryliové analogy 2,4,6-trisubstituovaných pyryliových solí mohou být vytvořeny reakcemi příslušných pyryliových solí se sulfidem sodným a následným vysrážením po přidání kyseliny; touto reakcí se kyslík v pyryliovém kationtu nahradí sírou.

Odkazy

Související články
 Šestičlenné aromatické kruhy s jedním atomem kruhu nahrazeným jinou skupinou: borabenzen, silabenzen, germabenzen, stannabenzen, pyridin, fosforin, arsabenzen, stibabenzen, bismabenzen, pyrylium, thiopyrylium, selenopyrylium, telluropyrylium

Reference

Kationty
Sirné heterocyklické sloučeniny

redpajama_set_name: RedPajamaWikipedia


Thai @ Home, the 1st of Chef Korn's cook book is a collaboration between SIAM CONNECTION SDN BHD and Seashore Publishing (M) Sdn Bhd (a member of Popular Holdings Limited). The cook book was launched on 20th August 2012 at the Popular Bookfest 2012 at Kuala Lumpur Convention Centre, KL.
This cook book was developed to cater for the demand of Thai cooking. The recipes are created for the "CHEF" to easily make simple Thai meals. Follow the simple steps to cooking and importantly savour it's delicious taste.


text:
Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells
Cheng Wei Chu, Huey Jiun Ko, Chia Hua Chou, Tai Shan Cheng, Hui Wen Cheng, Yu Hsin Liang, Yun Ling Lai, Chen Yen Lin, Chihuei Wang, Joon Khim Loh, Jiin Tsuey Cheng, Shean Jaw Chiou, Chun Li Su, Chi Ying F. Huang*, Yi Ren Hong
Department of Human Development and Family Studies
Thioridazine (THD) is a common phenothiazine antipsychotic drug reported to suppress growth in several types of cancer cells. We previously showed that THD acts as an antiglioblastoma and anticancer stem-like cell agent. However, the signaling pathway underlying autophagy and apoptosis induction remains unclear. THD treatment significantly induced autophagy with upregulated AMPK activity and engendered cell death with increased sub-G1 in glioblastoma multiform (GBM) cell lines. Notably, through whole gene expression screening with THD treatment, frizzled (Fzd) proteins, a family of G-protein-coupled receptors, were found, suggesting the participation of Wnt/β-catenin signaling. After THD treatment, Fzd-1 and GSK3β-S9 phosphorylation (inactivated form) was reduced to promote β-catenin degradation, which attenuated P62 inhibition. The autophagy marker LC3-II markedly increased when P62 was released from β-catenin inhibition. Additionally, the P62-dependent caspase-8 activation that induced P53-independent apoptosis was confirmed by inhibiting T-cell factor/β-catenin and autophagy flux. Moreover, treatment with THD combined with temozolomide (TMZ) engendered increased LC3-II expression and caspase-3 activity, indicating promising drug synergism. In conclusion, THD induces autophagy in GBM cells by not only upregulating AMPK activity, but also enhancing P62-mediated autophagy and apoptosis through Wnt/β-catenin signaling. Therefore, THD is a potential alternative therapeutic agent for drug repositioning in GBM.
Thioridazine
Dive into the research topics of 'Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells'. Together they form a unique fingerprint.
Thioridazine Chemical Compounds 100%
Cell death Engineering & Materials Science 94%
apoptosis Physics & Astronomy 88%
Catenins Medicine & Life Sciences 80%
Wnt Signaling Pathway Medicine & Life Sciences 72%
Autophagy Medicine & Life Sciences 61%
Glioma Medicine & Life Sciences 58%
drugs Physics & Astronomy 48%
Chu, C. W., Ko, H. J., Chou, C. H., Cheng, T. S., Cheng, H. W., Liang, Y. H., Lai, Y. L., Lin, C. Y., Wang, C., Loh, J. K., Cheng, J. T., Chiou, S. J., Su, C. L., Huang, C. Y. F., & Hong, Y. R. (2019). Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells. International journal of molecular sciences, 20(3), [473]. https://doi.org/10.3390/ijms20030473
Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells. / Chu, Cheng Wei; Ko, Huey Jiun; Chou, Chia Hua et al.
In: International journal of molecular sciences, Vol. 20, No. 3, 473, 01.02.2019.
Chu, CW, Ko, HJ, Chou, CH, Cheng, TS, Cheng, HW, Liang, YH, Lai, YL, Lin, CY, Wang, C, Loh, JK, Cheng, JT, Chiou, SJ, Su, CL, Huang, CYF & Hong, YR 2019, 'Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells', International journal of molecular sciences, vol. 20, no. 3, 473. https://doi.org/10.3390/ijms20030473
Chu CW, Ko HJ, Chou CH, Cheng TS, Cheng HW, Liang YH et al. Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells. International journal of molecular sciences. 2019 Feb 1;20(3):473. doi: 10.3390/ijms20030473
Chu, Cheng Wei ; Ko, Huey Jiun ; Chou, Chia Hua et al. / Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 3.
@article{81df6b63fa544a9fbe32f927b40e2c9e,
title = "Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells",
abstract = "Thioridazine (THD) is a common phenothiazine antipsychotic drug reported to suppress growth in several types of cancer cells. We previously showed that THD acts as an antiglioblastoma and anticancer stem-like cell agent. However, the signaling pathway underlying autophagy and apoptosis induction remains unclear. THD treatment significantly induced autophagy with upregulated AMPK activity and engendered cell death with increased sub-G1 in glioblastoma multiform (GBM) cell lines. Notably, through whole gene expression screening with THD treatment, frizzled (Fzd) proteins, a family of G-protein-coupled receptors, were found, suggesting the participation of Wnt/β-catenin signaling. After THD treatment, Fzd-1 and GSK3β-S9 phosphorylation (inactivated form) was reduced to promote β-catenin degradation, which attenuated P62 inhibition. The autophagy marker LC3-II markedly increased when P62 was released from β-catenin inhibition. Additionally, the P62-dependent caspase-8 activation that induced P53-independent apoptosis was confirmed by inhibiting T-cell factor/β-catenin and autophagy flux. Moreover, treatment with THD combined with temozolomide (TMZ) engendered increased LC3-II expression and caspase-3 activity, indicating promising drug synergism. In conclusion, THD induces autophagy in GBM cells by not only upregulating AMPK activity, but also enhancing P62-mediated autophagy and apoptosis through Wnt/β-catenin signaling. Therefore, THD is a potential alternative therapeutic agent for drug repositioning in GBM.",
keywords = "Apoptosis, Autophagy, Glioblastoma, P62, Thioridazine, Wnt/β-catenin",
author = "Chu, {Cheng Wei} and Ko, {Huey Jiun} and Chou, {Chia Hua} and Cheng, {Tai Shan} and Cheng, {Hui Wen} and Liang, {Yu Hsin} and Lai, {Yun Ling} and Lin, {Chen Yen} and Chihuei Wang and Loh, {Joon Khim} and Cheng, {Jiin Tsuey} and Chiou, {Shean Jaw} and Su, {Chun Li} and Huang, {Chi Ying F.} and Hong, {Yi Ren}",
note = "Funding Information: Funding: The study was supported by grants from the Ministry of Science and Technology (MOST) to YRH (Grant no: 107-2320-B-037-027) and C-YFH (MOST 103-2325-B-010-006 and 107-2320-B-010-040-MY3); other funding sources were Kaohsiung Medical University, Kaohsiung Medical University Hospital, and the National Sun Yat-sen University and Kaohsiung Medical University cooperative program (KMTTH-105-010, KMTTH-106-009, NSYSUKMU-106-P004 and NSYSUKMU-107-P006), which provided grants to YRH. Funding Information: The study was supported by grants from the Ministry of Science and Technology (MOST) to YRH (Grant no: 107-2320-B-037-027) and C-YFH (MOST 103-2325-B-010-006 and 107-2320-B-010-040-MY3); other funding sources were Kaohsiung Medical University, Kaohsiung Medical University Hospital, and the National Sun Yat-sen University and Kaohsiung Medical University cooperative program (KMTTH-105-010, KMTTH-106-009, NSYSUKMU-106-P004 and NSYSUKMU-107-P006), which provided grants to YRH. Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",
T1 - Thioridazine enhances p62-mediated autophagy and apoptosis through Wnt/β-catenin signaling pathway in glioma cells
AU - Chu, Cheng Wei
AU - Ko, Huey Jiun
AU - Chou, Chia Hua
AU - Cheng, Tai Shan
AU - Cheng, Hui Wen
AU - Liang, Yu Hsin
AU - Lai, Yun Ling
AU - Lin, Chen Yen
AU - Wang, Chihuei
AU - Loh, Joon Khim
AU - Cheng, Jiin Tsuey
AU - Chiou, Shean Jaw
AU - Su, Chun Li
AU - Huang, Chi Ying F.
AU - Hong, Yi Ren
N1 - Funding Information: Funding: The study was supported by grants from the Ministry of Science and Technology (MOST) to YRH (Grant no: 107-2320-B-037-027) and C-YFH (MOST 103-2325-B-010-006 and 107-2320-B-010-040-MY3); other funding sources were Kaohsiung Medical University, Kaohsiung Medical University Hospital, and the National Sun Yat-sen University and Kaohsiung Medical University cooperative program (KMTTH-105-010, KMTTH-106-009, NSYSUKMU-106-P004 and NSYSUKMU-107-P006), which provided grants to YRH. Funding Information: The study was supported by grants from the Ministry of Science and Technology (MOST) to YRH (Grant no: 107-2320-B-037-027) and C-YFH (MOST 103-2325-B-010-006 and 107-2320-B-010-040-MY3); other funding sources were Kaohsiung Medical University, Kaohsiung Medical University Hospital, and the National Sun Yat-sen University and Kaohsiung Medical University cooperative program (KMTTH-105-010, KMTTH-106-009, NSYSUKMU-106-P004 and NSYSUKMU-107-P006), which provided grants to YRH. Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
N2 - Thioridazine (THD) is a common phenothiazine antipsychotic drug reported to suppress growth in several types of cancer cells. We previously showed that THD acts as an antiglioblastoma and anticancer stem-like cell agent. However, the signaling pathway underlying autophagy and apoptosis induction remains unclear. THD treatment significantly induced autophagy with upregulated AMPK activity and engendered cell death with increased sub-G1 in glioblastoma multiform (GBM) cell lines. Notably, through whole gene expression screening with THD treatment, frizzled (Fzd) proteins, a family of G-protein-coupled receptors, were found, suggesting the participation of Wnt/β-catenin signaling. After THD treatment, Fzd-1 and GSK3β-S9 phosphorylation (inactivated form) was reduced to promote β-catenin degradation, which attenuated P62 inhibition. The autophagy marker LC3-II markedly increased when P62 was released from β-catenin inhibition. Additionally, the P62-dependent caspase-8 activation that induced P53-independent apoptosis was confirmed by inhibiting T-cell factor/β-catenin and autophagy flux. Moreover, treatment with THD combined with temozolomide (TMZ) engendered increased LC3-II expression and caspase-3 activity, indicating promising drug synergism. In conclusion, THD induces autophagy in GBM cells by not only upregulating AMPK activity, but also enhancing P62-mediated autophagy and apoptosis through Wnt/β-catenin signaling. Therefore, THD is a potential alternative therapeutic agent for drug repositioning in GBM.
AB - Thioridazine (THD) is a common phenothiazine antipsychotic drug reported to suppress growth in several types of cancer cells. We previously showed that THD acts as an antiglioblastoma and anticancer stem-like cell agent. However, the signaling pathway underlying autophagy and apoptosis induction remains unclear. THD treatment significantly induced autophagy with upregulated AMPK activity and engendered cell death with increased sub-G1 in glioblastoma multiform (GBM) cell lines. Notably, through whole gene expression screening with THD treatment, frizzled (Fzd) proteins, a family of G-protein-coupled receptors, were found, suggesting the participation of Wnt/β-catenin signaling. After THD treatment, Fzd-1 and GSK3β-S9 phosphorylation (inactivated form) was reduced to promote β-catenin degradation, which attenuated P62 inhibition. The autophagy marker LC3-II markedly increased when P62 was released from β-catenin inhibition. Additionally, the P62-dependent caspase-8 activation that induced P53-independent apoptosis was confirmed by inhibiting T-cell factor/β-catenin and autophagy flux. Moreover, treatment with THD combined with temozolomide (TMZ) engendered increased LC3-II expression and caspase-3 activity, indicating promising drug synergism. In conclusion, THD induces autophagy in GBM cells by not only upregulating AMPK activity, but also enhancing P62-mediated autophagy and apoptosis through Wnt/β-catenin signaling. Therefore, THD is a potential alternative therapeutic agent for drug repositioning in GBM.
KW - Apoptosis
KW - Autophagy
KW - Glioblastoma
KW - P62
KW - Thioridazine
KW - Wnt/β-catenin

redpajama_set_name: RedPajamaCommonCrawl


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Family day care units are homes where the people there supervise other kids along with their children. Space will be small in family day care when compared to other two. Toys will be of limited in numbers in family day care. Now, expenses of raising a child are very high, especially for small kids. For centers with high quality facilities and well educated care providers, we have to pay high expenses. Tuition is the main expense of a day care unit. Depending on the location and child age group, it varies.
Expenses are higher for younger kids as these groups need special care and supervision. For enrolling children of pre-school age, only amount is needed. Expenses depend on the hours of supervision also. Fees will be high, if hours extend. However, expenses are not really matter concerned. The matter concerning, is the matter of hygiene and quality education. Some units allow the children to watch TV all the time or else to sleep all the time. This is not right supervision. So parents must make sure all these.
If the expenses in a child care center are high, look for nearby family day care unit or else call for a nanny to look after a group of children and share the cost with other families. Palm Beach Child Care center is best for your children as they offer the best.


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Posted by cdcsolutions On January 5, 2021
In 2020, hackers around the world have increasingly gravitated to ransomware as their preferred method of attack. This year, there have been dozens of high-profile, successful attacks on companies around the world.
Whirlpool, one of the largest home appliance manufacturers in the world, is the latest company to fall victim to this type of attack.
The company is truly one of the titans in the industry, with nearly sixty locations scattered around the world and employing nearly 80,000 people. Unfortunately, their impressive size made them a tempting target. In their case, the hackers that breached their system utilized the Nefilim ransomware strain, managing to exfiltrate a variety of sensitive data before encrypting files on a number of company servers.
It has become increasingly common for hacking groups to publish some or all of the data they steal in a bid to hurt the company they attacked, and that's precisely what happened in this case. Thus far, the leaked data has included documents relating to accommodation requests, medical information requests, background checks, employee benefits and more.
There's no doubting that the publication of these documents are damaging to the company, but based on Whirlpool's investigation into the matter, the scope and scale of the data breach was quite small. Only a handful of corporate servers were infected and the files that were encrypted didn't impact the company's operations in any way.
It should also be noted that the hackers who control the Nefilim strain aren't terribly active, tending to only make occasional attacks targeting larger corporations. Other recent victims include Luxottica, The Dussman Group, Orange S. A. and the Toll Group.
At this point, all of the impacted Whirlpool servers have been fully restored, but the damage is done. It remains to be seen if the hackers behind the attack will publish more data, or if they've already released everything they stole. Stay vigilant. Your firm could be next.
iOS Update Includes iPhone And iPad Features And Fixes
Microsoft Releasing New Chromium Based Edge Browser To Users
Twitter Will Soon Release New Features With Update
Playing Videos Could Allow Hackers Into Your Phone
Microsoft Teams Has Added Several New Features
Fill out the form below to receive a free consultation and learn how we can make your technology worry-free!
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La classificazione SKIP dei kanji è un codice creato da Jack Halpern che permette di trovare un kanji per il suo aspetto, senza conoscere la pronuncia o il senso del carattere. SKIP è l'acronimo di System of Kanji Indexing by Patterns. Questo codice si presenta sotto forma di tre cifre separate da un trattino il cui primo, da 1 a 4, indica l'organizzazione generale del kanji.

Utilizzo

Prima cifra
La prima cifra può prendere il valore di 1, 2, 3 o 4 secondo la norma seguente:

 1 se il kanji è formato da due parti verticali
 2 se il kanji è formato da due parti orizzontali
 3 se il kanji è formato da due parti in cui una si inserisce nell'altra.
 4 se il kanji è formato da una parte unica

Seconda cifra
Se la prima cifra è 1, 2 o 3, la seconda cifra indicherà rispettivamente:
Il numero di caratteristiche della parte sinistra del kanji
Il numero di caratteristiche della parte alta del kanji
Il numero di caratteristiche della parte esterna del kanji.

Se la prima cifra è 4, si tratta del numero di caratteristiche totale del kanji.

Terza cifra
Se la prima cifra è 1, 2 o 3, la terza cifra indicherà rispettivamente:
Il numero di caratteristiche della parte destra del kanji
Il numero di caratteristiche della parte bassa del kanji
Il numero di caratteristiche della parte interna del kanji.

Se la prima cifra è 4, si distinguono ancora quattro casi e la terza cifra prenderà i valori seguenti:

 1 se il kanji sfoggia una caratteristica orizzontale verso l'alto.
 2 se il kanji sfoggia una caratteristica orizzontale verso il fondo.
 3 se il kanji sfoggia una caratteristica verticale che attraversa tutto il kanji dall'alto in basso.
 4 per tutti gli altri kanji.

Codici della forma 1-X-X

Codici 1-1-X

1: 八

2: 小  川

3: 心  水

4: 旧  必

7: 水

11: 順

Codici 1-2-X

2: 仁  仏  化  切  刈  双  比

3: 仕  他  付  仙  代  以  加  氷

4: 伐  任  仮  件  伝  仲  休  仰  伏  次  伎  州  伊

5: 伺  低  伯  位  作  但  何  似  体 伸  佐  伴  住  冷

6: 佳  侍  侮  例  価  使  依  併  供  協

7: 侯  係  保  便  俗  侶  俊  侵  促  信

8: 修  倍  倹  個  倫  俸  値  俵  倣  俳  借  准  候  凍  倒  帰

9: 偏  停  健  側  偵  偶  偽  頃  頂

10: 偉  傍  備  博

11: 傑  催  債  傷  傾  働  僧

12: 像  僕  僚

13: 儀  億

14: 儒  凝

15: 償  優

Codici 1-3-X

1:  孔  幻  引

2:  北  刊  収  幼  外  打  奴  払 巧  汁  功  犯

3:  兆  如  汚  叫  妃  池  吃  帆  壮  吐  忙  竹  吸  扱  羽  地  江  行  好  汗

4:  吟  改  批  吠  役  扶  吹  快  沢  坂  択  没  均  抜  沖  坊  折  沈  坑  抗  汽  妨  投  決  妙  抑  状  妊  		狂  岐  抄  阪  攻  技  防

5:  味  怖  披  泳  呼  性  抱  泌  坪  怪  抵  泊  姓  拓  抹  河  始  拘  押  沸  姉  拙  抽  油  妹  招  担  治  岬  拝  法  沼  弦  拠  泡  沿  径  拡  波  況  征  拒  泣  阻  往  拐  泥  附  彼  拍  注

6:  咲  恨  浄  垣  恰  派  城  悔  活  姻  挟  洪  孤  挑  津  峠  指  洞  峡  	持  洗  弧  拾  洋  後  拷  狩  律  括  独  待  海  狭  恒  浅  限

Codici della forma 2-X-X

Codici 2-7-X

4: 悪

Codici della forma 3-X-X

Codici della forma4-X-X

Esempi

町 = 1-5-2
 parte sinistra: 田
 parte destra: 丁

男 = 2-5-2
 parte alta: 田
 parte bassa: 力

匞 = 3-3-3
 parte esterna: 匚
 parte interna: 工

下 = 4-3-1

上 = 4-3-2

中 = 4-4-3

水 = 4-4-4

Lingua giapponese


Maheshwar is 40 kilometres away from district headquarter Khargone.and 90 KM from Indore city which is commercial capital of MP.
By Air : Nearest airport is Indore and named Devi Ahilyabai Holkar International Airport.Distance is 100 Km approx. Indore is a well connected to all the airports, and has almost direct flights to all important cities in the country.
By Train : Barwaha is the nearest railway station 40 Km from Maheshwar ( Indore-Khandwa-rail route ) at present due to broad gauge conversion of the track this is available from indore to barwaha only. and is not recommended till completion. may take 2 years may be ready in 2019.
By Road : Maheshwar is well connected through roads can be easily reached from Indore, Khandwa, Khargone, all close cities.
This city has its present significance & identity due to religious & generous nature and wisdom of Ahilyabai''s.She declared this holy city as capital,due to its glorious past.


Facioscapulohumeral muscular dystrophy affects the upper body. It is not the same as Duchenne muscular dystrophy and Becker muscular dystrophy, which affect the lower body.
Facioscapulohumeral muscular dystrophy is a genetic disorder due to a chromosome mutation. It appears in both men and women. It may develop in a child if either parent carries the gene for the disorder. In 10% to 30% of cases, the parents do not carry the gene.
Facioscapulohumeral muscular dystrophy is one of the most common forms of muscle dystrophy affecting 1 in 15,000 to 1 in 20,000 adults in the United States. It affects men and women equally.
Men often have more symptoms than women.
Facioscapulohumeral muscular dystrophy mainly affects the face, shoulder, and upper arm muscles. However, it can also affect muscles around the pelvis, hips, and lower leg.
Symptoms can appear after birth (infantile form), but often they do not appear until age 10 to 26. However, it is not uncommon for symptoms to appear much later in life. In some cases, symptoms never develop.
Shoulder muscle weakness causes deformities such as pronounced shoulder blades (scapular winging) and sloping shoulders. The person has difficulty raising the arms because of shoulder and arm muscle weakness.
Weakness of the lower legs is possible as the disorder gets worse. This interferes with ability to play sports because of decreased strength and poor balance. The weakness can be severe enough to interfere with walking. A small percentage of people use a wheelchair.
Chronic pain is present in 50% to 80% of people with this type of muscular dystrophy.
Hearing loss and abnormal heart rhythms may occur, but are rare.
A physical exam will show weakness of the face and shoulder muscles as well as scapular winging. Weakness of the back muscles can cause scoliosis, while weakness of the abdominal muscles can be the cause of a sagging belly. High blood pressure may be noted, but is usually mild. An eye exam may show changes in the blood vessels in the back of the eye.
A muscle biopsy is the removal of a small piece of muscle tissue for examination.
There is no known cure for facioscapulohumeral muscular dystrophy. Treatments are given to control symptoms and improve quality of life. Activity is encouraged. Inactivity such as bedrest can make the muscle disease worse.
Occupational therapy to help improve activities of daily living.
Oral albuterol to increase muscle mass (but not strength).
Surgery to fix a winged scapula.
Walking aids and foot support devices if there is ankle weakness.
BiPAP to help breathing. Oxygen alone should be avoided in patients with a high CO2 (hypercarbia).
Counseling services (psychiatrist, psychologist, social worker).
Disability is often minor. Lifespan is most often not affected.
Call your provider if symptoms of this condition develop.
Genetic counseling is recommended for couples with a family history of this condition who wish to have children.
Preston DC, Shapiro BE. Proximal, distal, and generalized weakness. In: Daroff RB, Jankovic J, Mazziotta JC, Pomeroy SL, eds. Bradley's Neurology in Clinical Practice. 7th ed. Philadelphia, PA: Elsevier; 2016:chap 27.
Sarnat HB. Muscular dystrophies. In: Kliegman RM, Stanton BF, St. Geme JW, Schor NF, eds. Nelson Textbook of Pediatrics. 20th ed. Philadelphia, PA: Elsevier; 2016:chap 609.
Warner WC, Sawyer JR. Neuromuscular disorders. In: Azar FM, Beaty JH, Canale ST, eds. Campbell's Operative Orthopaedics. 13th ed. Philadelphia, PA: Elsevier; 2017:chap 35.


How to track customer email support metrics with gmail?
We're using Gmail to field customer support requests. I am looking for a way to track key metrics of how we serve customers: average response time, %% of inquiries left unanswered, customer "happiness" meter.
Any ideas how to accomplish this? I've tried to search Google Marketplace and haven't found anything good.
Are you using any form of CRM system at the moment does it have any of the metrics you are after if so it maybe fairly simple to extract that info and show in GMail?
We use a CRM system called Capsule (though any good CRM should do what Capsule does for us) it has a Gmail/Apps integration for adding users into the system automatically allowing cases/opportunity etc.
We then use a separate platform called Rapportive, to provide Capsule information back to ourselves with a custom raplet, so the sidebar shows the users open cases/opportunity plus history which we have added. By using the CRM as the centre of our universe but Gmail as the shell to access it we have a lot of statistics (mostly very custom to us) such as user purchases, who last dealt with the customer, last interaction, when the next follow up was meant to be (and if over due) we also have a small happiness widget to indicate quickly if the user was last deemed to be happy (again something we whipped up in an afternoon).
With this information being custom to us it made sense to make it ourselves (our CRM integrates in 3 separate sales ledgers, and numerous support mechanisms as well as email/IM/telephone sales conversations all of this is being put in via the API), though through Raplets we were able to quickly build a platform to see the data, the key part was realising for us Gmail wasn't the place to store the data in the first place but a dedicated CRM.
2 Should I move my business email to Gmail?
3 CRM with email integration and useful views for support?
3 How do I handle customer support in foreign languages?
4 How would a company have telephone customer support without the agents hating their jobs?


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Saathi Ma Timro
Saathi Ma Timro is a Nepali movie directed by actor Bhuwan KC which depicts the story of a struggling producer and his son. The movie also touches the sensitive issue of father-son relationship. The character of the son, as a child is played by child artist Shirshak Sapkota. The movie has Bhuwan KC and Shrishak Sapkota on the lead roles with the special appearance of Anmol KC, Bhuwan KC's son.
The movie presents the story of a struggling producer named Avishek who wants to fulfill his father's dream but on his journey, he loses everything, his house, his car, his luxurious life including his wife Puja (Richa Ghimire) and is left with his son Anmol (Shrishak Sapkota) who is unaware of these incidents. He is on his way of fulfilling his father's dream and is set to produce the movie named Sathi Ma Timro giving it his hundred percent and turning it into a masterpiece rather than just another film. During this period, a struggling actress Ashmi (Rekha Thapa) gets attracted to him, however, fails to express her feelings for him. It is for audiences to find out if Avishek will be able to fulfill his father's dream or not and if Ashmi will be able to express her feelings or not.
The movie with a heart touching story is enjoyable and the audiences will stay glued to their seats until the end of the movie. The music director has done an appreciable job and so has the cinematographer. The dialogues are touchy and effective making the movie good in terms of presentation, story and performance. However, with its strengths, too, the movie fails to excite the younger generations. Moreover, the end of the movie might as well disappoint you. The movie has all the elements required for a mainstream Nepali movie and the major strength in the movie is the perfect chemistry between the two leading characters, Avishek and his son Anmol. The movie with all shades of emotions is a good watch if you want to catch up on some emotional Nepali flick.
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Loss of heterozygosity in the short tandem repeat (STR) loci found in tumor DNA of ALL patients as a factor predicting poor outcome
National Research Center for Hematology, Moscow, Russian Federation (Russia)
Genetic instability of tumor cells can lead to a change in the profile of short tandem repeats (STR) of the tumor relative to the STR profile of healthy cells of the patient. Loss of heterozygosity or allelic imbalance in STR loci confirm the chromosomal aberrations revealed by standard cytogenetic analysis in chromosomes containing this STR locus: deletions, monosomies, duplications, and the appearance of isochromosomes. However, in some patients, the loss of heterozygosity (LOH) is also observed with a normal tumor karyotype. Our objective was to evaluate correlations between the loss of heterozygosity in blast cells of de novo diagnosed ALL patients and chromosomal aberrations revealed by standard cytogenetic analysis, as well as to verify hidden abnormalities in the case of discrepancies between cytogenetic data and molecular analysis of the STR profile of the tumor.
Due to some restrictions caused by COVID quarantine the control points schedule was insignificantly delayed.
A study of a cohort of patients with ALL for the loss of STR-markers heterozygosity was performed in two periods: May-June and August-September 2020. Chromosomal microarray (CMA) was done in September-October 2020. The CMA results of LOH boundaries and genes involved in the field of uniparental disomy, deletions and duplications were analyzed and statistical evaluation of the loss of heterozygosity impact on overall and event-free survival was made in September-October 2020. Analysis of STR profiles of tumor cells was performed in 88 patients with de novo diagnosed Ph-negative ALL undergoing treatment according to the RALL-2016 protocol at the National Research Center of Hematology (Moscow, Russia).
The patients were 18-55 years old, 53 men and 35 women. DNA was isolated from bone marrow samples taken from patients at diagnosis. Control DNA samples were taken from the blood of patients in complete remission and/or from the buccal epithelium. STR profiles for each pair of tumor/control samples were obtained by multiplex PCR using the COrDIS Plus kit (Gordiz, Moscow), followed by fragment analysis of PCR products on an ABI 3130 genetic analyzer (Thermofisher Scientific, USA). Chromosomal microarray (CMA) of DNA with a detected loss of heterozygosity in STR loci was performed at the Genomed labs (Moscow, Russia) molecular pathology laboratory using the Genoscan 3000 system.
Normal cytogenetic tumor karyotype was established for 37 patients, and abnormal karyotypes were found in 51 cases. When analyzing the STR profiles of DNA from tumor and healthy cells of each patient, we found a loss of signal from one of a pair of alleles at heterozygous STR loci in twenty-one patients (24%). Seven of them had a normal tumor karyotype. Also, in the case of an abnormal STR karyotype, the loci with loss of heterozygosity did not always belong to aberrant chromosomes. Of 14 people with an abnormal tumor karyotype, for 8 the analysis of STR-profile revealed an allelic imbalance in loci, in which the CMA method was confirmed additional chromosomal aberrations not detected by cytogenetic analysis. And for 6 people LOH in STR loci confirmed by CMA coincided with cytogenetic analysis data. In this way, analysis of STR profiles revealed additional disorders in 15 out of 88 people(17%). In these 15 patients with CMA, aberrations up to 190 million base pairs (more than 1000 genes) were found. In the majority of patients with changes in the STR-profile on CMA, additional chromosomal abnormalities were found that were not identified by cytogenetic analysis and analysis of STR-profile.
One of these abnormalities was uniparental disomy of the shoulder or the entire chromosome, which was cytogenetically normal, but carried an STR locus with loss of heterozygosity. We issued the frequency of LOH and chromosomal aberrations found by CMA. The most frequent was LOH at 12p about 38% (8 from 21 patients) identified by CMA as a deletion in 25%, duplication in 6%, and uniparental disomy in 6%. Patients with the LOH at the short arm of chromosome 12 (12p) are excluded from the risk group because 12p LOH is not associated with worse outcome according to some publications [Takeuchi et al Leukemia 2003, Kawashima-Goto et al Int J Hematol. 2015].
LOH at 9p was also most frequent in ALL group. It was confirmed by CMA as a deletion in 38% (8 from 21 patients) and uniparental disomy in 19% (4 from 21 patients). *Clinical case: For one patient with a normal karyotype and STR-marker LOH, chromosomal microarray showed the microdeletion at 9p21.3 (21656682_22304230) affected the region of uniparental disomy at 9p (24.3-13.3). As a result of two of these chromosomal aberrations leading to a homozygous form of deletion, a cluster of MTAP, CDKN2A-AS1, CDKN2A, CDKN2B-AS1, CDKN2B genes containing three oncosuppressor genes involved in the regulation of antiproliferative and proapoptotic activities of Rb1 and p53, was completely absent from the tumor cell genome. The patient died of the disease. LOH 9p seems the most unfavorable marker, but it occurs in combination with other LOH that complicates the evaluation of LOH 9p as an independent risk factor.
LOH is a possible independent cofounder for Relapse Free Survival and Overall Survival of ALL patients. The null hypothesis – the loss of heterozygosity isn`t associated with the worst relapse-free and overall survival. Multivariate survival analysis was used to assess the independent impact of the new risk factor against the background of the other standard factors for this cohort of patients. Based on the preliminary obtained data, we expected the risk group according to the criteria for loss of heterozygosity would be 15-20% of the entire cohort. But in our project LOH group was 24% (21 patients from 88). OS and EFS analysis had shown, that the LOH presents a significant risk factor for dismal prognosis (Hazard Ratio= 4.1 (ci95 1.1-15.6)) (Fig.1)
Figure №1 Overall survival estimates for stable STR-profile (blue line) and LOH (red line) patients
We also tried to evaluate LOH 12p as a risk factor. Overall survival in the LOH 12p group was 100%. LOH 12p seems to the marker of preferable prognosis, but the group with LOH 12p only (without any other LOH) is too small (three people) for statistical significance evaluation.
Figure №2 Overall survival estimates for stable STR-profile (green line), LOH (red line) and LOH 12p (blue line) patients
Analysis of STR-profile in the tumor cells confirms and supplements the data of cytogenetic analysis, and also reveals an abnormal karyotype in the absence of mitoses in the sample. Moreover, LOH detection in cases of normal tumor karyotype reveals the phenomenon of uniparental disomy, hidden chromosomal aberration, which is often the cause for the transition of oncogenic mutations to homozygous form, thus aggravating the prognosis of the disease. The study of LOH 12p as favorable and LOH 9p as worst prognostic factors requires research on an expanded sample of patients.
The results were presented at the Society of Hematologic Oncology 2020 annual meeting on September 9-12, 2020 (poster presentation), prepared for III Congress of hematologists of Russia (canceled) , presented at XIV Raisa Gorbacheva Memorial Symposium "Hematopoietic Stem Cell Transplantation. Gene and Cellular Therapy" and at XXVII International Scientific Conference of Students, Postgraduates and Young Scientists "Lomonosov" 10 – 27 Nov 2020 (poster and oral presentation, https://cloud.mail.ru/stock/mUcEsH1wDX4aaPGE6ACymvHL). The original articles are being prepared for publication, but due to force majeure (restrictions associated with covid-19), everything has been delayed.


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Nutshell helps workers reclaim "social solitude" by shrouding them in fabric
Tamlin Magee | 20 November 2014 15 comments
Design student Eden Lew has created a prototype for a portable fabric pod called Nutshell that can be used to encase the top half of the body when time out from the workplace is required.
Part of Eden Lew's work for the Products of Design course at New York's School of Visual Arts, Nutshell was designed for students and workers who habitually skip proper work breaks as a device to encourage them to rest.
"We need to redefine the sleep cycle" says architect Jürgen Mayer H
When fully extended, the product surrounds the user in fabric from the torso and up over the head, supported by circular ribs, concealing them inside a canvas "shell".
Available in three different styles, it is wearable and collapsible, meaning that it can be used throughout the day for activities like snatching naps or eating lunch in solitude.
Nutshell has been developed in several varieties – the Nutshell Go is a simple model and can be pulled over the wearer's head. Nutshell Black is similar except that it is made from opaque material that plunges the user into a dark, warm and isolated space, while Headshell is a padded hood that is worn over the shoulders like a backpack.
Lew's inspiration for Nutshell stemmed from her own personal experiences with a hectic lifestyle.
"I'm a new New York transplant from Texas and the culture shock has been incredible," Lew told Dezeen. "I was swept away by the city's never-ending activity and graduate school has been fast-paced and demanding."
"I forget to eat meals because I never want to stop designing, and I'm constantly surrounded by excitement and activities," she said. "The Nutshell became my way of finding some personal peace and quiet, and I bet there are others out there who feel the same way."
As part of the project, Lew created a concept enterprise called Nutshell Labs to promote "social solitude" – her idea that embracing seclusion does not have to be a lonely act, but a supported one. The designer also drafted an accompanying conceptual app that would offer guided meditation and curated podcasts for the user to listen to from within the Nutshell.
Describing the user experience, Lew told Dezeen that the prototypes work "pretty well".
"The Nutshell Go is fun to wear and easy to pull over the head," she said. "When inside, I feel vulnerable yet safe, however I've only worn it in the studio around my peers. I've fallen asleep countless times in the Nutshell Blackout – it's called Blackout for a reason."
"If I create more Nutshells I'll have to figure out ventilation, it's a little warm in there," Lew added.
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I just finished a meditation by Sarah Blondin, one of my definite faves on Insight Timer. Her guided meditation called Make It Sacred is about making the little things in life have a sacred meaning. It doesn't mean every single moment is special or holy, but rather to take a few pauses throughout the day to have space. To be grateful for the things that are there, working. Your eyes for seeing. Your body for providing a container for your Self. Keeping you safe. If there are struggles with these things, look for others. Turn your face to the sun, she says. Feel that and know it is good.
In the midst of things seeming overwhelming, like too much, this can be especially challenging. As I write this, the holidays are in full effect. It is Christmas Eve. I know people are alone, sad, not with people they want to be with. With people they'd rather never share space with again. It can be so much. At times like this, I think a gratitude practice is maybe one of the most soothing activities we can engage. Put attention on the small things. Create them if they are not immediately apparent. Nature, your physical surroundings or your physical self can all be inspiration. Play music you like. Read. Watch funny videos on YouTube.
In 2011, One Thousand Gifts came out. The book was a pretty big deal on the circuit of self-help, spirituality and personal growth. I read it then, and was certainly moved. As with a lot of things, though, it was at the front of my mind for awhile, then life kept on. While reflecting on gratitude, though, and how to practice, I was remembering this book. I went to the website to see what was there, and found a reader created list of things for which they were grateful. There were big things and little things, and it was an excellent reminder of how to practice gratitude. I encourage you to look at it, and take or leave the Christian aspect as you see fit. The idea is so valuable beyond the scope of formal religion.
My last recommendation in the general gratitude department is giving the 365 Gratitude Journal app a try. It can be gamefied, if that's your jam. It's a journal with prompts for specific things to consider, as well as a place to collect what you're grateful for in a digital jar. You level up as your participation increases. It is also somewhat of a social network, so you can see what others are posting and gather inspiration from them. I am looking forward to seeing how influential the practice and prompting will be!
How do you practice gratitude in your daily life? Is it all about the small things? Do you keep track in a journal or app? Share your favorite practices!


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Posted inMorning Report, News
Morning Reporter: Filner's Attendance Record
by Colin Weatherby October 5, 2012 March 15, 2022
After six years with VOSD, our reporter Rob Davis is leaving Friday and he's made a pretty hefty proposition to our readers: Donate $10,000 by this weekend and he will donate his last week's pay.
Donate here if, like Rob, you want to help fund the future of investigative, in-depth journalism in San Diego.
Fact Check: Is Filner a Chronic No-Show?
People understand what it means when you don't show up at work.
Mayoral candidate Carl DeMaio is hoping to get some mileage out of this logic in his campaign against Rep. Bob Filner for San Diego's top political office. "Sadly, Bob Filner has one of the worst attendance records in Congress. Missed nearly 60 percent of the votes this year alone," claims the narrator in a recent DeMaio advertisement attacking Filner's record.
He's right — Filner's seat in the nation's capital spent a good part of the last two years collecting dust.
DeMaio used this approach with success against Assemblyman Nathan Fletcher in the primary and he's hoping for a repeat knockout. He's counting on the simple wisdom that if you don't show up for your job, maybe a job ought not show up for you.
But like most rhetoric on the campaign trail, things are not exactly what they seem. Reporter Liam Dillon ran the accusation through his Fact Check decoder and discovered some interesting details about the delicate dance in balancing a campaign with political responsibility.
Hedges Are Hedges, Unless They're Art
Sometimes landscaping is more than just a bunch of bushes. In this case, it is one of San Diego's most highly anticipated new art installations.
Local artist Robert Irwin made his mark in public art displays all over Southern California and now his work will take center-stage at the new federal courthouse downtown in the form of a zig-zagging garden. VOSD has been monitoring the progress over the past few months and reporter Kelly Bennett got to witness the planting earlier this week.
The lobby of the new building will also display a decades-old Irwin sculpture that never found a permanent home. The current project is likely to be one of Irwin's final large-scale endeavors, one of many built in San Diego during his nearly 40-year-long career in the creation of public art.
San Diego Crime Trends in 12 Graphics
Sometimes a little historical context tells a better story than any piece of news. Reporter Keegan Kyle compiled, crunched, and visualized 60 years of San Diego crime history data, revealing some interesting trends in the numbers.
Using the FBI's crime index and information provided by the San Diego Police Department, Kyle compared local statistics to national trends in violent crimes and property crimes. It quickly becomes apparent why San Diego is routinely named in lists of America's safest major cities, a far cry from notoriously rough years in the early 1990s.
Be sure to check out all of Kyle's recent infographics here.
Reader Comments: Protecting the City Budget
We published a letter from reader Chris Brewster advocating for a shift in San Diego's fiscal planning. In his letter, Brewster takes a look at the returns on city pension investments and makes the conclusion that more long-term planning could serve to alleviate some of the city's financial woes.
A wide variety of provocative letters from our readers have been published in recent weeks. Write in with your own comments and analysis of San Diego and be part of the conversation.
DeMaio Not Straightforward on Meetings with Manchester
Candidate Carl DeMaio wasn't exactly telling the truth when he said he had never communicated with U-T San Diego owner Doug Manchester, according to KPBS.
In a personal calendar acquired by KPBS, it was revealed that DeMaio had two meetings scheduled with U-T owner Doug Manchester in December and May, shortly before the paper endorsed DeMaio in his bid for mayor. These findings conflict directly with DeMaio's recent claim that he had only once met with the editorial board of the U-T, not Manchester. DeMaio quickly acknowledged that he had met with Manchester, although he claims campaign issues were the only item discussed.
Plan Submitted to Restart San Onofre
The Los Angeles Times reports that one reactor at San Onofre nuclear power plant will stay offline until next summer, despite a proposal submitted by Southern California Edison to restart one of the two reactors.
The plant was shut down earlier this year after equipment problems. We detailed some of the problems with the aging plant in an in-depth investigation and a San Diego Explained segment back in August.
Lavish Spending at San Diego Anti-Terror Center
An anti-terrorism center in San Diego was cited in a U.S. Senate report on Wednesday for its excessive spending, reports the U-T.
The state-operated center purchased 55 flat-screen televisions, claiming that the purchase was necessary for monitoring the news. The former director of the center was fired after spending nearly $75,000 on the purchase. The TVs were meant to be used for a training program that never happened, and were instead used to display calendars.
Throw in a few sixers, a bowl of peanuts, and a couple of Chargers posters and we might have San Diego's best new sports bar, right? OK, maybe not.
Colin Weatherby is a freelance writer. You can reach him at colin.weatherby@gmail.com and follow him on Twitter at @CCWeatherby.
Tagged: Bob Filner, Carl Demaio, Chris Brewster, Doug Manchester, Federal Bureau Of Investigation, Mayoral Candidate, Nathan Fletcher, Robert Irwin, United States Senate
Colin Weatherby
Colin Weatherby is a freelance writer. You can reach him at colin.weatherby@gmail.com and follow him on... More by Colin Weatherby


Iranian hit squads have been exposed for plotting a string of assassinations of political rivals in exile and other foes residing in Europe.
Don Lemon's contention that a person's race predisposes him to act violently is unequivocally racist. It's also easily debunked.
If Europe believes in enforcing blasphemy laws in order to suppress offensive speech is necessary for maintaining 'religious peace,' it's dead wrong.
September 11, 2001 is woven into the city where my son grows and learns, and it is a story he needs to hear. Here's what I tell him.
The Palestinian government could choose to take care of its own people and has the money to do it, but instead chooses to fund terror.
The evidence known to date, including some just discovered, strongly suggests the Wahhaj family had turned their rural compound into a terrorist training camp.
Despite these candidates' less than stellar polling results, there's reason for concern that efforts to legitimize Islamist politicians will continue.
If you value human life, freedom, and progress, then you should stand with Israel—as well as with everyone else in the region who seeks genuine freedom.
Whether it's a loss of moral clarity or a cudgel against Donald Trump, when it comes to Israel and the Palestinians, the press is recklessly political.
Muslims and well-meaning allies must be willing to have a conversation on how monolithic representations of Islam and Muslims alienate a vibrant Muslim subculture.
Iran is a breeding ground for terrorist activity, funding and controlling a global network of jihad terror organizations. Their containment can't end with the Iran deal.
The Trump administration ought not to concede one inch to those who wish to sideline the personnel and stifle the policies that would make its counterjihadist agenda a reality.
Reports of anti-Semitic violence in France rose by 26 percent last year, which has led record numbers of Jews to emigrate to Israel.
One of my best friends, a Pakistani Catholic named Michael, was brutally assaulted by Muslim thugs in a suburb of Karachi this week.


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entssouthwales
Entertainment news for South Wales
Review: Disney's Bedknobs & Broomsticks, Wales Millennium Centre, Cardiff
Posted byThomas Howells February 3, 2022 Posted inReviews, Theatre, UncategorizedTags:Cardiff, February 2022
Thomas Howells goes "bobbing along" to Wales Millennium Centre to review Disney's stage presentation of Bedknobs & Broomsticks!
A storyline that starts off frighteningly sad before evolving into something positively humorous and wholesome is the best way to describe the stage version of Disney's Bedknobs & Broomsticks, currently playing at Cardiff's Wales Millennium Centre.
The live musical successfully captures the feeling of animation, (just as the original live action Disney film successfully utilised), via puppetry and technical wizardry, despite all the obvious limitations of being a stage production.
The set of Disney's Bedknobs & Broomsticks adapts and builds itself around the characters, which is impossibly effective and appreciable! Credit: Johan Persson
Personally, one of my favourite features of this musical is how the set adapts and builds itself around the characters, which is impossibly effective and appreciable!
I hadn't seen the original film and the dark, but fun story is not what I was expecting. Set in the Second World War, it's a relatively sad story but with a lot of hilarious parts to break the tragic tone. Despite how entertaining it is, I imagine it could be scarier to younger audiences during certain scenes.
The scenes where the cast literally "do magic", are nothing short of incredible! I have no idea how they managed it! They were continuously eerie and yet beautiful, inducing an indescribable feeling. It seems totally impossible to do such things as move inanimate objects through windows and doorways on a live stage!
Dianne Pilkington as Miss Eglantine Price tries out a broomstick in the stage version of Disney's Bedknobs & Broomsticks. Credit: Johan Persson
The lighting is generally pretty dark the whole play, moreso in act 1. For me this felt fitting to the story, when it was set in a very literal dark period of the early days of World War II.
The puppeteering they use for the animal characters who appear is really animated and always astounding (while maintaining an air of cuteness) whether it tries to be funny or powerful!
Dianne Pilkington and Charles Brunton really manage to capture their characters in funny and beautiful ways, making Miss Eglantine Price and Professor Emelius Browne's individual stories feel far greater than not just anybody else could do. The three actors as the recently evacueed Rawlings children (led by Conor O'Court as Charlie) can be thanked for adding to the excitement and wonderment generated by the musical's animated feeling.
Disney's Bedknobs & Broomsticks continues at Wales Millennium Centre from until 5 February 2022, Credit: Johan Persson
Disney's Bedknobs & Broomsticks is such a fun and outstanding experience, not only for those seeing it for the first time, but for those old enough to know and love the original film (as the appreciative gasps of wonderment from the more mature children in the audience proved).
Disney's Bedknobs & Broomsticks is at Wales Millennium Centre from until 5 February 2022, visit www.wmc.org.uk for booking details.
Dianne Pilkington discusses Bedknobs & Broomsticks along with creatives. Read the full interview here!
Published by Thomas Howells
The doctor said I should be in hospital for all the blood I was losing, but instead I went to drama practice View more posts
Rock To The Golden Hits of Yesteryear with Lipstick On Your Collar in Newport
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1. The Confederate Approach on Harrisburg: The Gettysburg Campaign's Northernmost Reaches by Cooper H. Wingert (The History Pr, 2012).
As first glance, this is a very appealing book -- well illustrated, with good Heiser maps, and all indications are that it is thoroughly researched. This and Scott Mingus's Flames Beyond Gettysburg should form a nice pairing (Mingus wrote the foreword to Wingert's book). Among other things, Wingert covers the defensive preparations of Harrisburg, the fighting at Oyster Point and Sporting Hill, and the bombardment of Carlisle. While having gone no further than page flipping, I would venture that most Gettysburg students would want to grab a copy of this.
2. Confederate Incognito: The Civil War Reports of "Long Grabs," a.k.a. Murdoch John McSween, 26th and 35th North Carolina Infantry by Murdoch John McSween, edited by E.B. Munson (McFarland, 2012).
During the war, McSween wrote more than 80 letters to the Fayetteville Observer under the pen name "Long Grabs". His correspondence covers fighting in Virginia and North Carolina, as well as experiences training conscripts in his home state.
3. Colonel Edward E. Cross, New Hampshire Fighting Fifth: A Civil War Biography by Robert Grandchamp (McFarland, 2012).
Grandchamp, the author of several works of Rhode Island military history, here offers a biography of Edward Cross, who fought well in many great eastern theater battles before being mortally wounded at Gettysburg. It is a warts and all look at the man's life, with the book suggesting that he was an alcoholic with a combative personality off the battlefield, as well.
Chris Evans December 7, 2012 at 12:52 PM
Colonel Cross has fascinated me over the years. I think he had some of the best last words of any officer in the war, "I think the boys will miss me."
'My Brave Boys: To War with Colonel Cross and the Fighting Fifth' by Mike Pride and Mark Travis is a excellent bio of Cross mixed with a unit history of the 5th New Hampshire through Gettysburg.
'Stand Firm and Fire Low: The Civil War Writings of Colonel Edward E. Cross' has some excellent writings from Cross himself. It is interesting how critical he is of Meagher and the Irish brigade.
Mike Pride December 14, 2012 at 12:14 PM
Thanks, Chris. If you liked "My Brave Boys," you might also like my latest book, "Our War: Days and Events in the Fight for the Union." Among other things it includes new material on Cross and the Fifth, including a firsthand account of his death that Mark Travis and I did not have when we wrote MBB.
Robert Grandchamp, for whose Cross biography I wrote the foreword, is full of new information about the colonel. Robert is a dogged researcher.


It hardly felt like I'd left Mull when I drove off of the ferry and turned right to head towards Salen and on to Loch Na Keal. The trip in July for the inaugural Photo Tour on the island had been a roaring success both in terms of capturing images of the wildlife promised on the itinery and also for everyone who had gone along, who we had become firm friends with.
This fortnight was mostly going to be a break though, and having been on Mull so recently, I already had a very good idea of where the wildlife would be. That said, at the usual layby for watching the white-tailed eagles on Loch Na Keal, there were none in the trees. One was however, across the water being divebombed by a black-backed gull, as it fed on something washed up on the shore.
Further along the road, I caught sight of something of a disturbance in the calm surface of the water, parked up and realised quickly that there was an otter fishing there. Within moments I had donned my camo jacket, attached the monopod to my camera and was hot-footing it across the uneven ground to where I'd seen it surface. With a more careful approach closer to the otter, both for the sake of spooking it and for me not slipping over on the seaweed, I was soon as close as I thought I needed to be, and as well hidden as possible amongst the rocks. Silent Mode activated on the camera, and it was time to wait.
The otter was in front of me, mooching around in a pool of seaweed, almost cut off from the main creek. Every so often it would surface, and look around. The pool must have been shallow, as I could see the otter lifting the weed as it searched the area for food, until it burst up pretty close. I took a couple of shots, and in the calm conditions, it was clear the sound of the camera had carried, when the otter froze and looked in my direction.
I froze too, and after a few seconds, the otter seemed to relax and continued looking for its dinner.
Eventually it climbed out of the water and I could see that it had quite some bulk to its back; pregnant, I assumed.
The otter then spent quite some time rolling around in the seaweed on some rocks, drying her fur and grooming. With her busy with such activity I was able to take more images, before I decided to leave her be, and quietly creep away again. She was still snoozing on the rock when I arrived back at the car.
Heading south again, we didn't see anything much worth stopping for, apart from our neighbour for the fortnight, a friend from Birmingham who had been tempted to see what Mull had to offer after seeing my images all the time in the local country pub!
The evening seemed to be drawing to a close when a gap between the cloud and the horizon let the sunlight flood through, and we all emerged from the cottages and hotel to watch the sunset.
My friend Reg, walks his dog very early each morning at home, so it was no surprise to me to find I wasn't first out of the cottage car park on the first morning. After waving at him as I drove around to the marshes, I clocked something out of place on the top of a hillside, and soon realised it was a white-tailed eagle. I waited for the sun to reach it, but really it was a bit too high up for anything worthwhile.
Autumn was already starting to show with the hawthorn trees laden with berries, gratefully received by the local hooded crows.
Calm conditions again meant it was easy to spot otters fishing, and I found another along the road, fishing just off shore. Once again, I scuttled out, and followed it along the lochside. When I realised it had finished fishing, and was heading ashore, I managed to get ahead of it, and tucked myself into some rocks, near where I thought the otter would come out, and head into a holt. All seemed to be going well when it scrambled out of the water, just out of sight for a shot, along the beach area. Then it headed back in again, and vanished from sight. Was it going past me? Damn, I thought. Then I heard a crunching sound from next to me.
Bearing in mind my lens has a minimum focusing distance of about 5 metres, it wasn't much help when I found myself staring at a similarly surprised otter, only about 4 feet from me, in the rocks I had chose to hide in! There was a moment of both of us looking at each other in bewilderment, before the otter turned tail and shot off back into the water! If only I'd been wearing some sort of head-cam.
After collecting Dad from the digs, we continued on a tour around the area, and I soon spotted another otter, dragging something large ashore to feed upon. By now the sun was strong and I noticed that there was quite some shimmer between me and the otter. Annoying, as it seemed to be eating a huge lobster!
I crept as close as I dared, but being in an exposed spot meant I soon attracted a crowd, and the sound of car stereos and doors slamming, soon disturbed the otter, which vanished out into the seaweed-covered water. I've lost count of the number of otters I have seen spooked by people who don't realise they need to be quiet in such situations, and attempt to be discreet when viewing. But such is life, and the otter had eaten most of the lobster before it decided to leave.
The first full day ended with me watching distant hen harriers hunting over the marshes. I truly believe I am cursed when it comes to getting something really good of these birds, and this was proved during the week when Reg, out walking his dog, managed to get some full-frame images of a ringtail. I spent hours in the same area later that week and failed to see anything.
The Monday was slightly unusual for me, in that one of the clients from the July tour was also on the island, and had asked if I minded guiding her for a half day. I didn't, but it meant a very early start to reach her digs on the other side of Mull. I really should have stopped along the way, as the sight, and colours of the sunrise over the Sound Of Mull and later over the entrance to Loch Na Keal was jaw-droppingly beautiful. What a place.
After enjoying a great trip out with Martin on the eagle trip, my client was keen to see some otters, and hopefully add to her image collection from the July tour. It didn't take long to spot one, but it was fishing way off shore, and I couldn't see an easy way to get close, without being too exposed.
I chose to head to another spot where I had seen some the day before. As we drove closer, it was clear that another photographer was already working that patch, so I parked up some distance away, and we carefully approached the area, where we had both seen an otter fishing. I don't like treading on other photographer's toes, so to speak, so I suggested we watch from a sensible distance and see what happened. The otter headed ashore near where the other guy was hiding, I had to admit I felt envious.
But we chose to be patient, and it soon became clear that perhaps the envy was based on the wrong assumption that he'd got a great view. Maybe he had initially, but suddenly we saw the otter scampering over the rocks towards us, whilst looking over its shoulder, back at something. The photographer had perhaps lost sight of the otter, and had climbed up for a better view. We both grabbed a couple of shots of the otter before it dived back into the water.
I guessed that it would head towards where we'd parked, so we made our way along the shore, found a good viewing spot that provided some good cover, and waited.
Result, the otter came out of the water right next to us. I took a couple of shots, but I was acutely aware of the sound of my camera, even on Silent Mode, and made sure I paused if the otter looked at where we were hiding. My client's 7D Mark2 was on Silent Mode too, but that actually is silent, so she happily snapped away.
Back out into the water, and fishing for a while longer, we followed it further along the shore, where it again came out, this time to groom and dry off. It was far enough away not to be concerned about the sound of the cameras too.
The half-day had to be ended (probably well over the half-day in terms of time) as my client needed to get to the ferry to leave. Another successful guiding session on Mull, and long may it continue.
Back to my holiday for the remainder of the trip and a trip out to Croggan yielded some good views of a pair of golden eagles, and a family of spotted flycatchers near one of the cottages.
No otters again on that loch - I think Andy cursed it when he said it had provided him with a 100% success rate for seeing them, and we have not seen one there since!
As is usual for Mull, buzzards seem to be everywhere, and while some might choose to ignore them, I love to take advantage of the chances for images of them, especially on natural perches. There's even a very pale individual that has been around for some years now, that posed one morning for me. Gorgeous.
Our first trip out with Martin on Mull Charters came mid-week, and I had my doubts about the forecast. Reg also came along, though he admitted he didn't really have sea-legs. Needn't have worried for the start of the sailing, as it was reasonably calm, and we had a white-tailed eagle come over almost as soon as we'd left the new jetty.
Unfortunately the "take" was facing away from us, and as the trip progressed, the weather deteriorated, ending with most of us crammed in the cabin to shelter from the driving rain. Reg amused us, as he repeatedly banged his head on the door frame when turning to talk to us. I don't think he was quite as amused as I was though.
The following day brought another otter sighting, and I managed to get a few more shots. Plus we encountered a most obliging buzzard, perched on the top of a roadsign. Even when we drove up to it, it remained perched. I reversed back, and took some shots, only seeing the bird spooked when another driver came towards us, and pushed the bird off to another post.
Having spent many an hour touring around the far side of the loch each morning, and even over to the next one, I was amused to spot a pair of otters almost within walking distance of the cottage. They had climbed out on to some rocks and were so absorbed with whatever one was eating, they didn't notice me approaching from the road. Tucking myself into a cleft in the rocky shore, I ensured I was in a position close enough, but not too close to risk missing shots of the pair if they moved apart slightly.
It was great to watch the pair finding plenty to eat in the loch, and then feeding on the catches on the rocks. One even started to explore the area which brought it much closer to me.
The day ended sharing a meal and a few drinks in Reg and Yvonne's cottage, though it was interrupted when the ever-changing light on Mull coaxed us outside for a few shots of the unusual sunset.
The following morning was dead calm, and while we both drove around the marshes and on to the loch, I caught sight of a young common seal lying just out of the water. Unfortunately, as Reg parked up behind, the seal slid back into the water. Watching from our cars, we saw it soon clamber back out, and we spent the next hour or so photographing it, as it dried out on some rocks.
Initially it was a touch too far really, but the calm weather meant the loch was like a mirror.
Then, while we watched from the cover amongst the bracken and heather on the shore, the seal relocated back to the same closer rock once more, and we were able to get some lovely images.
I moved around quietly to ensure the water, and hence reflections were dark, which made for a good contrast with the seal, which was gradually becoming paler as it dried off.
The day ended with me receiving some broken voicemail messages from Reg. He'd found a young hedgehog wandering around in the road, and had taken it back to the cottage nearby, to relocate it somewhere safer. I'd missed this, and also when he offered it a plate of dog food, which it apparently scoffed down! Putting out a second plate, on a track beside the car park, he'd hoped it might have found its way off away from the road.
I was initially gutted, especially when I got back to find Dad had photographed it, and didn't know where it had gone. I've not had the chance to photograph a hedgehog since I bought my DSLR gear back in 2006! I used to have some visit my garden but they, like so many others, have vanished.
Thankfully, I spotted Reg whilst driving out again, and he explained where to look and sure enough, there was the 'hog, pottering along a nearby track.
Amusingly, after hoping it would vanish into the woodland nearby, it chose to climb back into the garden, and we watched it scuttle about the lawn, searching around the plant pots for more to eat. How it could be hungry after two plates of dog food is anyone's guess.
It eventually skulked off into some bushes, and we hoped it would be ok. It was rather small, and I worried about it being large enough at this time of year. But I wasn't sure if there were any facilities on Mull for looking after hedgehogs, so it would have to rely on nature looking after it.
A thoroughly miserable day followed, with pouring rain. I still forced myself out and was initially rewarded with a fine double rainbow over the marshes.
And then on the way back, I spotted the pair of otters again, and grabbed some shots as they ran over the rocks together.
When the rain finally eased, Dad and I parked up where the otters had been, hoping for a view. Whilst waiting, I was aware of several ravens calling, and my Spider-Sense tingled. Sure enough, I was right to be alert, as Fingal, the local white-tailed eagle soared into view, just skimming the tree tops behind us.
I thought that it was about time we had some memorable encounters with one of the eagles, as we had on so many previous trips, but that didn't happen until the following morning. Across the loch, I noticed something sat on the rocks some distance away, and both Reg and I parked up to look. A white-tailed eagle.
Just then, another flew overhead, and perched in a tree across a field from where we'd parked. Reg went for a closer look, whilst I watched on. It soon took off, circled over us and headed towards where we had seen the other. Reg went one way, I chose to go look for the original one on the rocks, and as I did, I heard a horrible cry from a grey heron. Like the sound they make when fighting... or being killed by an eagle, in this case.
I can't be absolutely sure as the kill was made out of sight, but soon the two eagles had become four, and after abandoning my idea of approaching the one on the rocks, Reg joined me on the headland, where we watched the four flying overhead.
Two of them, sub-adults from what we could tell, were unhappy with one another and briefly fought in the skies, before circling, chasing each other, right over our heads.
It was fantastic to see, and way too close at times to keep both in the shot.
The adults from the nearby nest had also come down for a feed, and we saw both leaving the area shortly after.
By the time I'd returned back to the cottage to collect Dad, the skies had cleared and it was a glorious day. We both thought it warranted a drive south, to look across at Iona. That stretch of water is so beautiful, especially on a day like we saw it.
Time was flying by and our second trip on the Lady Jayne had arrived. Again it was overcast, but dry at least, and the light wasn't at all bad. And we were treated to a number of takes from the eagles, at two of the nest sites Martin and Alex sail out to.
As in the summer, the preferred combo of Canon 1DX and my old original 100-400mm zoom lens worked perfectly, tracking the eagles as they circled, descended and grabbed the fish.
And the drive home was productive when we encountered another otter.
Yet another miserable wet day followed, with gusty winds and monsoon-like rain. I just sat in the car and watched the wildlife close to the cottage, including grey and pied wagtails, and a small band on dunlins and ringed plover feeding from the shore.
And so arrived the last day. After seeing the local otters on my drive back to the cottage, I collected Dad and we returned to watch. I didn't hold much hope of getting any shots given the number of observers at the site, all of which were standing on the road or just off it, in plain sight of everything, including the otters. Needless to say, the otters kept away, and when a boat moored up right beside them, dropping out some lobster pots, the otters vanished completely, along with the crowds.
I drove down the road, and waited, and, after making sure everyone had gone, we returned, and waited some more. It was a good move, as one of the otters had obviously been hiding amongst the rocks, and once the audience and boat had left, it came back out to fish, and by then, I was kneeling close to the water's edge, in a swamp... so glamorous!
But worth it, when the otter climbed out to clean and groom, only a few yards from me.
Back into the water soon after, and then it seemed to head back up a small stream. I lost sight of it, and was about to return to the car when I saw it in the water near me still. Time to freeze, and move once it had dived again. It caught quite a large crab and needed to be ashore to handle it. Thankfully I was just able to get to a spot to photograph it from, but I was also aware that two people who had left before, were now back, and as before, were standing like beacons on the edge of the road.
The otter could plainly see them, and left some of the crab lunch to head back to the water again.
The beacons then left once more and after a while, the otter approached the edge of the loch, climbing out with yet another crab to eat. I relocated, and while not as close, still enjoyed the sight and sound of the otter crunching through the catch.
A pair of hooded crows pestered the otter, and even stole some of the food. This pushed the otter back into the shallows, and for a moment I thought I was going to get a second close encounter of the otter kind. But the traffic on the nearby road kept the otter at bay, and I could see it watching from beneath the cover of the seaweed.
Followed by yet another otter encounter shortly afterwards. To be crouched watching the otter fishing in a calm loch, in sunny, mild weather was heavenly, and I felt sick to have to drag myself away to drive home. Even the pouring rain that had arrived as we waited in the queue for the ferry didn't wash away the feeling.