At Gapforce, we are passionate about travel and the positive impact it has on young people's outlook and future prospects. Our mission is to help people become global citizens through authentic, socially and environmentally responsible travel experiences which aid self-discovery and personal development.
Through our range of school group trips, gap year programs, training courses and internships, we aim to provide our participants with a unique collection of experiences designed for different stages of life.
Program Advisor: Your initial point of contact will usually be one of our program advisors who are well-travelled themselves and passionate about the benefits that travel brings. Their job is to make sure you have all the necessary information to enable you to make an informed decision about which adventure is the right one for you.
Operational Support: Once you've decided on your adventure and booked your place, you will connected with a dedicated member of our operations team who's job is to guide you through the planning and preparation stages and makes sure you have everything you need before you go. Attention to details is their specialty and they go to great lengths to ensure a smooth pre-departure experience.
Leadership Team: This is our team looking after you on the ground and made up of Expedition Leaders on our group travel programs and suitably experienced local staff on our dive courses and internship programs. They are the ones responsible for making sure your program runs smoothly and safety and we have gone to great lengths to source the best in the business, a further commitment being that the majority of our group travel leaders have graduated from our intensive four month Expedition Leader Training Course.
Local Businesses: we work with authentic local service providers selected on account of their strong codes of practice and commitment to customer satisfaction.
Community Projects: projects are sourced and vetted locally to ensure they are needed by the local community, aims and objectives clear and achievable, and there is a plan in place to ensure the longevity of the project.
Environmental Projects: We only work with recognised organisations with a strong background in sustainable environmental and wildlife conservation, and a clear and unambiguous goal for the future.
Gapforce is committed to the highest levels of safety and security. We cannot remove risk from overseas travel altogether but by following a clear set of processes and management systems, we aim to reduce your level of risk compared to independent travel in the same location.
Industry Standards: To guide us, we follow the standards set by BS8848 "Specifications for visits, fieldwork, expeditions and adventurous activities outside the UK". Gapforce is also a Duke of Edinburgh Approved Activity Provider (AAP), and we are members of Year Out Group who set guidelines and policy for UK Gap Year providers.
Safety Policy: We have a detailed safety policy which sets out how we identify and manage risk and starts from your initial enquiry right the way through to your program and evaluation after. This is a dynamic policy which is regularly reviewed and updated as part of our continuous improvement strategy.
Financial Bonding: Gapforce (London) Ltd is a company committed to customer satisfaction and consumer financial protection. We are therefore pleased to announce that, at no extra cost to you, and in accordance with ''The Package Travel, Package Tours Regulations 1992'' all passengers booking with Gapforce (London) Ltd are fully insured for the initial deposit, and subsequently the balance of monies paid as detailed in your booking confirmation form. The policy will also include repatriation if required, arising from the cancellation or curtailment of your travel arrangements due to the insolvency of Gapforce (London) Ltd. This insurance has been arranged by Towergate Chapman Stevens through Zurich Insurance PLC.
More questions? Don't worry, just call us now 1-888-249-4533.


text:
Monsanto website downed as Anonymous claims hack
screenshot from http://www.monsanto.com
Hackers from the Anonymous collective claimed to have infiltrated the website for the biotech giant Monsanto, which has been the subject of recent international protests.
The cyber-attack was carried out as part of #OpMonsanto, an attempt to demonstrate against Monsanto's reach into every aspect of the food industry, from nature to farmers to consumers. The corporation's main website, Monstanto.com, appeared to have been briefly disabled Wednesday night.
It comes days after over two million people took part in rallies in 436 cities in 52 countries on May 25.
The organizers of the May 25 rallies call for labeling of GMO foods and further scientific research on their health effects. They also urge supporters to "vote with their dollar" by buying only organic products and boycotting Monsanto-owned companies. Besides that, they are urging a repeal of the so-called Monsanto Protection Act passed in the US Congress, and that the company's executives and politicians who back them are held to account through "through direct communication, grassroots journalism, and social media."
'Monsanto Protection Act' might be repealed in Senate
Vermont could be first US state to mandate GMO food labeling
Purchase power: App allows votes against Monsanto, for GMO labeling
Challenging Monsanto: Over two million march the streets of 436 cities, 52 countries

redpajama_set_name: RedPajamaCommonCrawl


text:
Close 'em all...
Close 'em all... Sept 8, 2010 6:56:29 GMT -5
Typical one-size-fits-all response to a problem.
"Crack Cave, which is on the Comanche National Grassland near the Colorado-Oklahoma border, is a tourist magnet. People come for the cave's unique rock art, which is illuminated only during equinoxes.
Jeff Stoney, grassland's district ranger, said in a press release there is no alternative, but to close the cave.
He said while there are no records of bats utilizing Crack Cave, the emergency order covers all caves and abandoned mines even if bats are not known to inhabit them. The order was signed July 27."
chieftain.com/news/local/article_f159c732-b4c0-11df-bb0a-001cc4c002e0.html


text:
package pgit

// Pgit is an instance of Pgit that is bound to a specific schema
// directory where the database schema is located and a particular
// database connection.
type Pgit struct {
	db     DatabaseConnection
	schema *schemaDirectory
}

// New initializes and returns a new Pgit instance
func New(rootPath string, db DatabaseConnection) (*Pgit, error) {
	schema, err := newSchemaDirectory(rootPath)
	if err != nil {
		return nil, err
	}
	return &Pgit{db: db, schema: schema}, nil
}

// ApplyLatest ensures the latest version of the schema has been applied
// to the database, and if not applies it.
func (p *Pgit) ApplyLatest() error {
	return p.schema.applyLatest(p.db)
}

// Rollback rolls back the last migration that was applied
func (p *Pgit) Rollback() error {
	return p.schema.rollback(p.db)
}

redpajama_set_name: RedPajamaGithub


text:
Q: Android Populate ListView from database, format textview with two decimals I have this method that populates ListView from Database. Everything works good. But I want to have one of the textfields showing the value with two decimals.
This has been asked before and many marked is as duplicate but the question is how can I implement the two decimals and where do I do it in the code below
As you can see, I tried many different things without success. Maybe I need to build a custom adapter where I set my TextViews?
And please don't mark this as a duplicate because the answer is not given for etc here:
Best way to Format a Double value to 2 Decimal places
or here:
Round a double to 2 decimal places
or here:
How to round a number to n decimal places in Java
Here comes my code and my question is where can I Implement my code for rounding up to two decimals?
private void populateListViewFromDB(int month, int year) {

    Cursor cursor = dbHandler.getMonth(month,year);

    // TextView setEx = (TextView)findViewById(R.id.oneRowExkl);
    // set = Double.parseDouble(setEx.getText().toString());
    // setEx.setText(String.format("%.2f", set));
    //TextView ex = (TextView)findViewById(R.id.oneRowExkl);
    //ex.setText(String.format("%.2f", Double.parseDouble(MyDBHandler.INKL)));

    String[] fromFieldNames = new String[] {
            MyDBHandler.COLUMN_PRODUCTNAME, MyDBHandler.DATE,
            MyDBHandler.INKL };
    int[] toViewIDs = new int[] { R.id.oneRowName, R.id.oneRowDate,
            R.id.oneRowExkl };

    // Create adapter to may columns of the DB onto element in the UI.
    @SuppressWarnings("deprecation")
    SimpleCursorAdapter myCursorAdapter = new SimpleCursorAdapter(this, //
    Context
            R.layout.one_row, // Row layout template
            cursor, // cursor (set of DB records to map)
            fromFieldNames, // DB Column names
            toViewIDs // View IDs to put information in
    );

    ListView myList = (ListView) findViewById(R.id.list1);
    // myList.setBackgroundColor(Color.GRAY);
    myList.setAdapter(myCursorAdapter);
}

A: First, what is the datatype in the DB? Is it set to handle decimals/stored as such in DB? Can you change the rounding rules on the DB side?
If not, you want to implement the rounding in code. Since you are using a SimpleCursorAdapter in your example, it uses convertToString(Cursor) to output data to your views.
What you can do is give it a custom string converter, that you create, for the specified column, where you can do the rounding.
For example:
CursorToStringConverter myConverter = new CursorToStringConverter() {

@Override
public CharSequence convertToString(Cursor cursor) {
   int myColumn = [whatever column id];
   String s = cursor.getString(myColumn); //Or .getFloat() for instance
   return [whatever rounding you want, or truncating, or such]
 }
};

Then set it on your adapter:
myAdapter.setCursorToStringConverter(myConverter);

You can also try giving the adapter a new ViewBinder, where you check for the desired column and when it is about to be bound, you manually edit the output before binding, like this (paste this in before you assign the adapter to the view):
myAdapter.setViewBinder(new ViewBinder() {
@Override
public boolean setViewValue(View view, Cursor cursor, int columnIndex) {

    if (columnIndex == 3) {
            //If it is actually stored as float number in db, do
            //cursor.getFloat(columnIndex) instead
            String formatStr = cursor.getString(columnIndex);
            TextView textView = (TextView) view;

            //Here, do your truncating
            [insert whatever code to either truncate string or format float]

            textView.setText(fixedStr);
            return true;
     }

     return false;
 }
});

To just see how it works, before doing your truncating or formatting, where it says [insert...] just set:
String fixedStr = "Random string";

and you'll probably see what it does.

A: Just to show how the problem was solved with @Richar Erikssons code, here's the code that solved my problem
 myCursorAdapter.setViewBinder(new ViewBinder() {
         @Override
         public boolean setViewValue(View view, Cursor cursor, int
         columnIndex) {
             if (columnIndex == 3) {

                     TextView textView = (TextView) view;

                     String fixedStr = String.valueOf(String.format("%.2f",
                     cursor.getDouble(columnIndex)));

                     textView.setText(fixedStr);
                     return true;
              }

              return false;
          }
         });

redpajama_set_name: RedPajamaStackExchange


The A21 is the UK's most dangerous stretch of road. It is a key trunk road for the constituency linking us to the M25, London and airports. It is managed by Highways England. It is wholly unacceptable that such a key route it is not a dual-carriage way from start to end. Villages along the A21 such as Hurst Green experience noise, congestion and accidents.
Whilst I welcome the extension of the dual-carriageway between Pembury and Tonbridge that will open this summer, we now need the same investment to be delivered along the whole length of the A21 through Bexhill and Battle and onto Hastings.
As a member of the A21 Reference Group of MPs and local council representatives, I have been actively lobbying the Government and Highways England to continue road improvements south of Tonbridge especially at key pinch points like Hurst Green. I have recently written to key partners including County and District councils South East LEP and Highways England to ask for their views and support in applying to the government's new Housing Infrastructure Fund. The Fund has been created to support infrastructure projects which deliver much-needed housing.
As major road improvement projects can take a long time to deliver, I am also pressing Highways England to come up with a strategy to make the A21 safer for all road users which can be implemented as soon as possible.
Last year I attended a meeting with local residents, councillors and Highways England to hear concerns about the increased traffic congestion on the A259 in Little Common. Traffic has increased by 20% during peak times since the Bexhill to Hastings Link Road was opened in December 2015.
Residents told me that traffic is often at a standstill on Barnhorn Road and the increased volume is affecting quality of life in the area. Housing developments have been approved that will adjoin the A259 in Little Common and residents are worried about the increased volume of cars accessing the trunk road and related safety issues.
Many residents would like to see the former Bexhill by-pass plans to be reconsidered to relieve traffic pressure on this area. I therefore wrote to key local partners and Highways England to establish whether a major by-pass road scheme could be re-considered, and whether the Housing Infrastructure Fund would be a possible source of funding.
The view from key partners was that Little Common would have to accept far higher housing numbers than currently proposed in the Local Plan in order to attract this road infrastructure funding. This is therefore a matter for our local councillors and residents to consider as part of a longer term plan for the area. For the immediate future, I will continue to press Highways England for measures that will improve the safety and traffic management of this road.
The A27 is a key route for the south coast and, as any regular commuter between Bexhill and Brighton will know, the section of road between Polegate and Lewes is congested, slow and prone to accidents. This is bad for the local economy and impacts on the lives of people who live and work in East Sussex. I have therefore joined with neighbouring MP and local representatives in calling the government to help secure funding to create a dual carriageway to alleviate the bottleneck on this part of the South East's strategic road network.
Local MPs Amber Rudd and Huw Merriman were delighted to see a Budget announcement of a further £420 million of new money being allocated to local highways maintenance, including funding to the local council.


Claire Foy: I play Erin who's on a gap year before university and goes off to Israel with her schoolfriend Eliza, who is doing her National Service. Erin is very moody. She feels her life is not as good as anyone else's and has got a massive chip on her shoulder. But when she reads her grandfather Len's diary she starts to change. It's about his experiences in post-war Israel and all of a sudden she feels she has something to believe in and someone to love.
Christian Cooke: And I play her grandad in the scenes set after the war. Len was part of the British peacekeeping force sent to Israel in accordance with the 1917 mandate. He's young and naive but he's got a strong sense of justice.
Did you heavily research the roles?
Claire Foy: No, we left that to Peter Kosminsky who wrote the drama. He was out in Israel for a year. Having said that, reading the scripts did make me open my eyes a bit more, to the extent that I now find it hard to think back to before filming because I learnt so much.
Christian Cooke: I didn't want to research it because my character knew nothing about the situation either. I had to imagine what an uneducated young man in Forties Palestine would be thinking of the situation. However there was a book called Cordon And Search: 6th Airborne Division In Palestine 1945-1948 which details every undertaking. It was fascinating.
Why do you think Len kept a diary?
Christian Cooke: I suppose you would call it a form of therapy. In those days, any therapy would be seen as showing a sign of moral fibre. I mean, I also think people who have been in those situations find it easier to bury it rather than articulate it.
Have either of you kept a diary before?
Claire Foy: I did when I was much younger. It's quite interesting to look back at what I was thinking at the time. I've changed a lot. Or at least I hope I have. I was a nightmare — so angry about everything. It's as if I came out of a coma when I was 21.
What was it like filming in Israel?
Christian Cooke: I missed everyone back home but I had an amazing time. There was a real sense of camaraderie with the rest of the cast. We hung out together a lot and it just felt like a real privilege to be out there filming for such a long time.


text:
An Interview with Entrepreneur Dan Huang
Dan Huang is a co-founder at Orca, a social selling enablement tool that helps inside sales teams take their sales process beyond just cold email and calls. He's on a mission to change the way organizations are...
An Interview with Entrepreneur & Angel Investor Robin Elenga
Robin Elenga is a serial Entrepreneur having founded and ran multiple technology-based companies including RevelHMI, Revel Body, MobileGIS, Trakleads, ETS Consulting. Robin is also an angel investor who invests in...
An Interview with Entrepreneur Alban Denoyel
Alban is a French entrepreneur living in Brooklyn. Alban is the co-founder and CEO of Sketchfab, the largest platform to publish and find 3D content online. Before Sketchfab, Alban worked for 4 years in the 2D...
An Interview with Entrepreneur Kishan Ananthram
Kishan Ananthram is a CEO of IonIdea & the Founder of GuardOn. In the following interview, Kishan Ananthram talks about his firm IonIdea & GuardOn, his entrepreneurial journey, struggles and the lessons...
An Interview with Entrepreneur Amit Varma
Amit likes solving problems. He has entrepreneurship experience in the banking, financial services outsourcing, and ITeS industry in India, and then in the energy industry in Canada, where he became pained with the...
An Interview with Entrepreneur Andrew Peterson
Andrew Peterson is the Founder of Clozd, world-class services and technology for win-loss analysis. Prior to founding Clozd, Andrew was the general manager for an enterprise software product line at Qualtrics....
An Interview with Entrepreneur Todd Fisher
Todd Fisher is the Co-Founder and CEO of CallTrackingMetrics, a top-rated call tracking & automation platform. Founded in 2012, CallTrackingMetrics has more than doubled in size every year and now serves over...
An Interview with Entrepreneur Noam Weisman
Noam Weisman is a cyber and telecom industries veteran that held multiple senior sales, BD and product positions with startups and large corporates. Noam's last position before he started his first company,...


Protected: Gryphons shouldn't marry. Vampires don't dance. A man who inherits a library card to the library in Alexandria.
This entry was posted in Uncategorized on November 30, 2014 by katemonkeys.
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This entry was posted in Uncategorized on November 21, 2014 by katemonkeys.
This entry was posted in Uncategorized on November 18, 2014 by katemonkeys.
Protected: Eh, kill her, give her cab fare, whatever.
This entry was posted in Uncategorized on November 15, 2014 by katemonkeys.
This entry was posted in Uncategorized on November 12, 2014 by katemonkeys.


Food Ingredients: Chinigura Rice, (Farm)Chicken, Coconut milk, Lemon grass Spices etc.
Descriptions: We are offering Malaysian Traditional Food " Chicken Rendang". It's exceptional but tasty. Try it! One order contains: Flavored Steam Rice 300gm (maximum) Chicken Curry- 3 piece.


text:
US Navy outlines discharge policy for unvaccinated sailors, includes possible punishments of recouping cost of training and education
Paul Sacca
Image source: Instagram/U.S. Navy
The United States Navy announced new details about its COVID-19 vaccine mandate that goes into effect next month. The Navy noted that a newly created panel that will oversee the administrative discharge process for unvaccinated sailors could recoup the cost of training and education for service members who refuse to get the coronavirus vaccine.
All active-duty sailors are required to be fully vaccinated against COVID-19 by Nov. 28. Service members in the selected reserve must be fully vaccinated by Dec. 28. Technically, sailors must have both doses of the COVID-19 vaccine or the one dose of the Johnson & Johnson by Nov. 14 so they can complete the 14-day waiting period to achieve full vaccination status by Nov. 28.
Those who refuse to get the vaccine by the deadline and do not have a medical or religious exemption will be discharged. Those service members will receive no lower than a general discharge under honorable conditions, which could result in the loss of some veterans' benefits.
To oversee the administrative discharge process for unvaccinated sailors, the Navy recently established the COVID Consolidated Disposition Authority. The CCDA has the authority to punish unvaccinated sailors by recouping the cost of training, education, and more.
"The CCDA may also seek recoupment of applicable bonuses, special and incentive pays, and the cost of training and education for service members refusing the vaccine," the U.S. Navy stated in a press release.
"For Navy service members refusing the vaccine, the CCDA also retains the authority for administrative processes regarding removal of warfare qualifications, additional qualification designations (AQD), Navy Enlisted Classifications (NEC), or sub-specialties, except in cases where removal authority is otherwise authorized by law or Executive Order," the Navy said.
Adm. William Lescher, Vice Chief of Naval Operations, said the CCDA would "ensure a fair and consistent process" for the discharge of unvaccinated sailors.
As of Wednesday, 94% of active-duty sailors are fully vaccinated and 99% have received at least one dose of the vaccine, according to the U.S. Navy. For reserve sailors, 72% are fully immunized against COVID-19 and 78% received at least one dose.
The Washington Post reported that 76.5% of active-duty Marine Corps personnel are vaccinated, and a mere 38% of Marine Corps Reserve personnel are vaccinated. There are reportedly 60,000 Air Force service members who are not fully vaccinated ahead of the branch's Nov. 2 deadline. Only about 40% of the Army Reserve and National Guard are vaccinated, but they have a vaccination deadline of June 30, 2022.
According to Navy data released on Oct. 13, there have been a total of 163 COVID-19 deaths within the branch, of which 102 were civilian employee deaths.
The U.S. Navy has 347,487 active duty personnel, 96,710 reserve personnel, and 281,161 civilian employees as of July 2021.
"Tragically, there have been 164 deaths within the Navy family due to COVID-19, far exceeding the combined total of all other health or mishap related injuries and deaths over the same time period," Vice Adm. John B. Nowell, Jr., the Chief of Naval Personnel, said on Oct. 14.
The Navy noted that of the 164 Navy COVID-19 deaths, 144 were not vaccinated. The vaccination status of the remaining 20 deaths remains unknown.
Military members speak against vaccine mandates with Massie ... ›
Members of the U.S. military now face mandatory COVID-19 ... ›
Canada ethics professor questions vaccine mandate in video ... ›
Hospital workers quit over vaccine mandate, maternity closes ... ›
news-reporter-fired-vaccine-mandate - TheBlaze ›


text:
<?php namespace solyluna\Http\Controllers\Characteristic;

use Illuminate\Support\Facades\Auth;
use solyluna\Amenity;
use solyluna\Http\Requests;
use solyluna\Http\Controllers\Controller;

use Illuminate\Http\Request;
use solyluna\User;

class CharacteristicsController extends Controller {

	/**
	 * Display a listing of the resource.
	 *
	 * @return Response
	 */
	public function index($id)
	{
		//id de la propiedad
		$property = $id;

		//usuario
		$user_id = Auth::user()->id;
		$user_role = User::findOrfail($user_id);

		//amenities
		$amenities = Amenity::where('property_id', '=', $property)
			->paginate(5);
		return view('admin.properties.characteristics.index',compact('user_role','property', 'amenities'));
	}

	/**
	 * Show the form for creating a new resource.
	 *
	 * @return Response
	 */
	public function create()
	{

	}

	/**
	 * Store a newly created resource in storage.
	 *
	 * @return Response
	 */
	public function store(Request $request)
	{
		$user_id = Auth::user()->id;
		$user_role = User::findOrfail($user_id);
		$characteristic = new Amenity($request->all());
		$characteristic->save();
		return view('admin.control.admin',compact('user_role'));
	}

	/**
	 * Display the specified resource.
	 *
	 * @param  int  $id
	 * @return Response
	 */
	public function show($id)
	{
		//
	}

	/**
	 * Show the form for editing the specified resource.
	 *
	 * @param  int  $id
	 * @return Response
	 */
	public function edit($id)
	{
		//
	}

	/**
	 * Update the specified resource in storage.
	 *
	 * @param  int  $id
	 * @return Response
	 */
	public function update($id)
	{
		//
	}

	/**
	 * Remove the specified resource from storage.
	 *
	 * @param  int  $id
	 * @return Response
	 */
	public function destroy($id, Request $request)
	{
		$amenity = Amenity::findOrfail($id);
		$amenity->delete();
		if($request->ajax()){
			return response()->json([
				'id' => $amenity = Amenity::findOrfail($id)
			]);
		}
	}

}


Watch out America, a Next To Normal tour is currently in the works. And the first city it will visit........Chicago! It's slated for the 2010-2011 season. It was first mentioned in this Chicago Tribune article but confirmed by this July 2 Twitter post from a producer (Patrick) of Next To Normal.


The Microbial Fuel Cell (MFC) can be a future environmentally friendly biotechnological application for the production of electrical energy. As a future alternative energy source, the bioelectricity generation must become more efficient. A major limiting factor is the low bacterial membrane permeability, hindering transport of electron shuttles through the membrane and thereby restricting the electron shuttle-mediated extracellular electron transfer (EET) from bacteria to electrodes. This results in a reduced electrical power output of the MFC. Therefore, we heterologously expressed the porin protein OprF from Pseudomonas fluorescens into Escherichia coli.
Figure 1: Schematic overview of the enhancement mechanism of electron shuttle-mediated electron transfer between bacteria and the anode of MFCs by the synthetic porin OprF. Oxidized mediators diffuse into the periplasmatic space where they accept electrons. Reduced mediators are secreted through outer membrane porins and donate their electrons to the electrode.
Heterologous expression of the porin protein OprF from Pseudomonas fluorescens into Escherichia coli leads to a much higher current output in comparison to its parental strain (E. coli KRX). This is most likely caused by improved electron shuttle-mediated extracellular electron transfer through dramatically increased membrane permeability. The heterologous expression of the outer membrane porin OprF from Pseudomonas fluorescens in Escherichia coli is a great genetic strategy to overcome limitations due to the membrane and to increase electricity generation by microorganisms.
For the biobrick part, BBa_K1172501, there was a 24.3% of identity match and 51.3% similarity match to the top allergen in the allergen database. This means that the biobrick part is not of potential allergen status. In 80 amino acid alignments by FASTA window, no matches found that are greater than 35% for this biobrick. This also means that there is not of potential allergen status.
The oprF gene from Pseudomonas fluorescens was cloned and heterologously expressed in Escherichia coli KRX under the control of different promoters (Table 1).
Table 1: Overview of oprF devices. Combination of oprF coding BioBrick (BBa_K1172501) with different promoters and RBS. The characterization of this BioBrick was performed by comparing Escherichia coli KRX wild type with Escherichia coli KRX carrying BBa_K1172502, BBa_K1172503, BBa_K1172504, BBa_K1172505 and BBa_K1172507.
Upon expression of the outer membrane porin protein OprF, the morphological and physicochemical characteristics of the E. coli surface were analyzed. SDS-PAGE combined with MALDI-TOF MS/MS, different membrane permeability assays (NPN and ONPG), a hydrophobicity assay and Atomic Force Microscopy (AFM) characterize the OprF BioBrick BBa_K1172501.
OprF was detected in an SDS-PAGE with a broad overexpression band. Protein isolation of the outer membrane porin OprF by release of periplasmic protein fraction from E. coli via cold osmotic shock using Cell fractionating buffer 2.3 was successful.
Figure 2: SDS-PAGE with Prestained Protein Ladder from Thermo Scientific as marker. Comparison of protein expression between Escherichia coli KRX wild type and Escherichia coli KRX with BBa_K1172502, BBa_K1172503 and BBa_K1172507 after periplasmic protein fractioning with Cell fractionating buffer 2.3. The band used for the MALDI-TOF MS/MS is marked with an arrow.
The SDS-PAGE shows a significantly higher protein concentration in extracts from E.coli expressing OprF from a T7 promoter (BBa_K1172502). This is most likely caused by the higher membrane permeability (shown with NPN and ONPG uptake assay), allowing an increased release of membrane proteins by 0.2 % SDS. Nevertheless, a strong overexpression band can be observed at the expected OprF size of about 36 kDa for BBa_K1172502, which is equated with a strong expression and overproduction of OprF.
Furthermore we were able to identify the overexpressed outer membrane porin (Figure. 2) with MALDI-TOF MS/MS.
MALDI-TOF MS/MS of the gel band thought to represent OprF and analysis with MALDI-TOF MS/MS confirmed the outer membrane porin with a Mascot Score of 222 against the bacteria database.
In order to test the effect of the protein expression on the cell growth, growth curves were investigated.
Figure 3: Growth curves for E. coli KRX with LB medium. Comparison between Escherichia coli KRX wild type and Escherichia coli KRX with oprF plasmids BBa_K1172501, BBa_K1172502, BBa_K1172503 and BBa_K1172507. oprF gene expression was induced for inducable promoters at OD600 = 1.0. All data are representing two biological and two technical replicates with standard deviation.
E. coli KRX wild type shows the best growth characteristics with a maximal optical density OD600 = 4.0. E. coli KRX with oprF coding plasmid shows a slightly lower growth due to the higher replication stress. E. coli KRX expressing oprF under control of strong Anderson and lac promoters are showing 10% decreased maximal cell density and E. coli KRX expressing oprF under control of T7 promoter shows a further decreased growth which was 45% reduced in comparison to E. coli KRX wild type. This lower growth is based on an increased expression of oprF on the cellular surface and therefore on higher metabolic pressure, which was confirmed with different assays, which can be found in the further characterization of the outer membrane protein OprF.
The heterologous expression of the outer membrane porin OprF will enhance the hydrophobicity of cell membrane. The outward-facing side groups on each of the β-strands of the OprF monomer are hydrophobic. Therefore, heterologous expression should be detectable by an increase in hydrophobicity. The Hexadecane-Hydrophobicity-Assay is based on different affinities to hexadecane of dissimilar cell-types according to their cell surface. Hydrophobicity can be observed by specific changes in OD600 of the aqueous phase.
An increasing hydrophobicity of cell membrane changes the physicochemical properties of the cell. This could effect for example the cell-electrode interaction. Therefore, we investigated the cellular surface characteristic by comparing Escherichia coli KRX wild type with Escherichia coli KRX with BBa_K1172502, BBa_K1172503, BBa_K1172504, BBa_K1172505 and BBa_K1172507 (Table 2 and Figure 4).
The OprF strain shows an increasing affinity to hexadecane with increasing promoter strength in comparison to the wild type. Cells in which OprF was expressed from the T7 promoter (BBa_K1172502) showed the maximal hydrophobicity, which was three times higher than the affinity to hexadecane of the wild type.
Table 2: Results of the Hexadecane-Hydrophobicity-Assay. Comparison of hydrophobicity between Escherichia coli KRX wild type and Escherichia coli KRX with BBa_K1172502, BBa_K1172503, BBa_K1172504, BBa_K1172505 and BBa_K1172507. Affinity to hexadecane (hydrophobicity) with standard deviation and enhancement in comparison to the wild type is shown. Two biological and at least three technical replicates were analyzed.
Figure 4: Results of the Hexadecane-Hydrophobicity-Assay. Comparison of hydrophobicity between Escherichia coli KRX wild type and Escherichia coli KRX with BBa_K1172502, BBa_K1172503, BBa_K1172504, BBa_K1172505 and BBa_K1172507. Affinity to hexadecane (hydrophobicity) with standard deviation and enhancement in comparison to the wild type is shown. Two biological and at least three technical replicates were analyzed.
The enhanced hydrophobicity of OprF expressing strains indicates a successful expression of the outer membrane porin in Escherichia coli. Such an increased hydrophobicity on the outer membrane caused by the expression of OprF will lead to an increase in the cellular adhesion to the surface of the carbon anode and an enhancement of direct electron transfer from Escherichia coli to the electrode.
Besides testing the outer membrane hydrophobicity for physicochemical characterization of the E. coli surface, we measured the membrane permeability by fluorescence-based NPN (N-Phenyl-1-naphthylamine) uptake assay for outer membrane morphology characterization (Helander and Mattila-Sandholm, 2000).
NPN is a suitable chemical for measuring the membrane permeability of cells. An increasing NPN fluorescence intensity indicates an enhanced NPN uptake through the outer membrane and enhanced membrane permeability (Loh et al., 1984).
Figure 5 shows a higher fluorescence emission and hence higher membrane permeability with increasing promoter strength for OprF producing strains in comparison to Escherichia coli KRX wild type.
Figure 5: Results of the NPN-uptake-assay. Comparison of fluorescence emission between Escherichia coli KRX wild type and Escherichia coli KRX with BBa_K1172502, BBa_K1172503, BBa_K1172504, BBa_K1172505 and BBa_K1172507. Excitation at 355 nm with fluorescence emission scan from 320 to 390 nm wavelength and with standard deviation. Two biological and at least three technical replicates were analyzed.
Escherichia coli with heterologous expression of the outer membrane porin OprF shows more efficient NPN uptake than the Escherichia coli KRX wild type, suggesting an increased membrane permeability with increasing promoter strength. E. coli with oprF under T7 promoter control (BBa_K1172502) shows the maximal membrane permeability with 100% enhanced permeability in comparison to E. coli KRX wild type. The weak Anderson promoters seem unsuitable for heterologous expression of this gene.
Another way of characterizing the outer cell membrane can be achieved with an fluorescence-based ONPG assay. This assay measures the membrane permeability by whole-cell lactase enzyme activity (Zhou et al., 2010).
Electron shuttle-mediated electron transfer (EET) determines the transport efficiency of electron shuttles across the cell membrane. With the NPN uptake assay we were able to quantify the transport efficiency of chemical molecules across the cell membrane. In contrast, the ONPG assay (whole-cell β-lactase enzyme activity assay) measures not only the diffusion of ONPG into the cell but also the release of its hydrolytic product across the cell membrane. With the ONPG assay we are able to observe diffusion processes in and out of the cell membrane.
Due to the fact that heterologous expression of oprF in Escherichia coli should not have an impact on the expression of β-lactase, we can assume that the β-lactase activity of E. coli KRX wild type and E. coli KRX with oprF expression plasmid is identical. All in all, the ONPG assay provides significantly more information than the NPN uptake assay.
Figure 6: Results of the ONPG-uptake-assay. Comparison of ONPG hydrolysis between Escherichia coli KRX wild type and Escherichia coli KRX with BBa_K1172502, BBa_K1172503, BBa_K1172504, BBa_K1172505 and BBa_K1172507. Absorbance at 405 nm wavelength with standard deviation is shown. Two biological and at least three technical replicates were analyzed.
The ONPG hydrolysis rate by β-lactase is much higher for E. coli KRX containing OprF plasmids in contrast to E. coli KRX wild type. Whereas we could only observe a minimal hydrolysis activity in the wild type, E. coli KRX, carrying OprF expression plasmids, shows a much faster ONPG hydrolysis rate with increasing promoter strength and a maximal rate for E. coli KRX with T7 promoter (BBa_K1172502), which is 30 times higher than wild type hydrolysis rate.
In summary, we could prove that the heterologous expression of OprF from Pseudomonas fluorescens in Escherichia coli significantly improves the membrane permeability. The NPN and ONPG assay show comparable results. Outer membrane permeability of E. coli is enhanced with increasing promoter strength for oprF expression. The wild type shows only a weak uptake rate of chemical molecules (NPN) and no product release as quantified with ONPG assay. Therefore, the E. coli wild type is not well suitable for a usage in the MFC using mediators. In contrast, membrane optimized Escherichia coli with OprF shows increased diffusion across the cellular membrane, indicating a possible optimization of electron shuttle-mediated electron transfer (EET) to the anode and increasing current production.
In addition to the morphological and physicochemical characterization of the Escherichia coli outer membrane, we wanted to visualize the surface. The technique of choice for this is Atomic Force Microscopy (AFM). After cell preparation we were able to get AFM pictures of the E. coli surface with the help of the working group of Prof. Dr. Dario Anselmetti, with special help from Dr. Volker Walhorn.
Figure 7: Light microscopy of AFM layer after cell preparation of Escherichia coli KRX wild type.
Figure 8: Light microscopy of AFM layer after cell preparation of Escherichia coli carrying oprF under the control of T7 promoter (BBa_K1172502). Enhanced cell clustering, caused by higher hydrophobicity, can be observed.
Microscopy of the AFM glass layer after cell preparation, before AFM measurement, shows the different cell properties. Number of cells as well as magnification scale are the same. Escherichia coli KRX expressing oprF from a T7 promoter (BBa_K1172502) presents a clustering of cells, whereas the wild type forms a monolayer. Thus, the increased hydrophobicity of the cell surface is already visible even with an ordinary light microscope.
AFM was carried out using the MultiMode® 8 AFM from Bruker. The measurements were performed on air with 'Tapping Mode' and in water with 'Peak Force Mode'.
Figure 9: AFM images of the cell surface for Escherichia coli KRX wild type and Escherichia coli KRX with heterologous expression of porin OprF (BBa_K1172502). Images are shown in Topography and Contrast mode.
The heterologous expression of the porin OprF causes a slightly rougher membrane, the morphology and thus the viability of Escherichia coli is preserved to the greatest possible extent.
All results so far indicated the potential for an increase in energy production. This assumption was confirmed by cultivation of Escherichia coli KRX with OprF (BBa_K1172502) in comparison to Escherichia coli KRX wild type in the Microbial Fuel Cell.
Indeed, the extracellular electron transfer mediated by electron shuttles is improved in the OprF expressing strain resulting in an increased bioelectricity output (Figure 10 - 13).
Figure 10: Microbial Fuel Cell results from cultivation of Escherichia coli KRX with OprF (BBa_K1172502) in contrast to Escherichia coli KRX wild type. Voltage curve from Escherichia coli KRX wild type, Escherichia coli KRX expressing OprF (BBa_K1172502), M9 medium with Methylene Blue as mediator and M9 medium without mediator is shown over time and over a resistance of 200 Ω.
Figure 11: Microbial Fuel Cell results from cultivation of Escherichia coli KRX expressing OprF (BBa_K1172502) in contrast to Escherichia coli KRX wild type. Electric charge curve from Escherichia coli KRX wild type and Escherichia coli KRX carrying OprF (BBa_K1172502). M9 medium was used with mediator Methylene Blue and with measurement over a resistance of 200 Ω.
Figure 12: Microbial Fuel Cell results from cultivation of Escherichia coli KRX expressing OprF (BBa_K1172502) in contrast to Escherichia coli KRX wild type. Electric power curve from Escherichia coli KRX wild type and Escherichia coli KRX carrying OprF (BBa_K1172502). M9 medium was used with mediator Methylene Blue and with measurement over a resistance of 200 Ω.
Figure 13: Microbial Fuel Cell results from cultivation of Escherichia coli KRX expressing OprF (BBa_K1172502) in contrast to Escherichia coli KRX wild type. Average electric power bar chart from Escherichia coli KRX wild type and Escherichia coli KRX carrying OprF (BBa_K1172502). M9 medium was used with mediator Methylene Blue and with measurement over a resistance of 200 Ω.
Escherichia coli KRX with OprF (BBa_K1172502) shows 108 % higher maximal voltage than Escherichia coli KRX wild type with a maximum of 308 mV. Over the whole cultivation the voltage was improved by about 100 %. Tests using M9-medium with and without mediator Methylene Blue show no voltage, indicating that bioelectricity generation is solely due to the bacteria.
The calculation of the electric charge confirms the described results. Electric charge is equivalent to the number of transported electrons and is enhanced by 111 % for Escherichia coli KRX with OprF (BBa_K1172502). The maximal electric charge of 7,2 C demonstrates that heterologous expression of outer membrane porin OprF enhances extracellular electron transfer dramatically. High membrane permeability is crucial for efficient mediator transport across the membrane and therefore for high bioelectricity generation.
Finally, the calculated power curve shows 239 % enhanced electric power for Escherichia coli KRX carrying OprF (BBa_K1172502) with a maximal electric energy transfer rate of 475 μW and a 300% enhanced average electric power.
Cell membranes are protective layers, which are crucial for physiology of the bacteria. In consideration of the Microbial Fuel Cell, the cell membrane is also a barrier which decreases mediator and thus electron exchange between bacteria and anode. With heterologous expression of the outer membrane porin OprF from Pseudomonas fluorescens in Escherichia coli we were able to enhance membrane permeability. With this optimized cell membrane surface we generate an efficient electron shuttle-mediated EET with decreased limitation of EET by membrane barrier. In contrast to a perforation of the membrane with chemicals, cell viability is maintained with OprF expression (Liu et al., 2012). Beside improvement of EET, enhanced hydrophobicity shows optimized cell adhesion to the anode for biofilm formation and direct electron transfer.
The heterologous expression of OprF is a genetic strategy to optimize electron shuttle-mediated electron transfer as well as electricity generation in Microbial Fuel Cells. The most suitable and efficient OprF device for Escherichia coli is a combination with the rhamnose inducible T7 promoter (BBa_K1172502).
The funtionality of the outer membrane porin OprF (BBa_K1172507) in E.coli KRX ΔdcuB::oprF was investigated with a NPN-Uptake-Assay (Cheng et al., 2005).
Figure 14: Results of the NPN-Uptake-Assay. Comparison between Escherichia coli KRX wildtype and Escherichia coli KRX with BBa_K1172507, BBa_K1172502 and Escherichia coli KRX with genome integrated oprF gene (E.coli KRX ΔdcuB::oprF).
Figure 14 shows the results of the NPN-Uptake-Assay. Higher fluorescence emission and higher membrane permeability could be observed with increasing promotor strength for oprF. The genome integrated oprF shows mid-level fluorescence emission and membrane permeability. Highest fluorescence levels could be measured with the oprF gene on a high-copy pSB1C3 plasmid under control of the T7 promotor.
Genome integrated oprF showed almost the same membrane permeability as the constitutive expressed oprF with BBa_K1172507. There even seems to be a marginal higher expression of the genome integrated oprF in contrast to the plasmid coded oprF although the same constitutive promotor (BBa_J23104) is used. This could be explained by physiological condition of the cell. Constitutive expression of the high-copy oprF causes cell stress. Protein expression could be automatically downregulated by the cells. The single-copy genome integrated oprF showed a reduced expression level, therefor cell stress is also smaller. As a consequence E. coli cells showed a more effective expression of the outer membrane porin OprF adjusted on physiological cell condition. So we constructed a stable E. coli strain, which shows constitutive expression of outer membrane porines OprF because of genome integration of oprF gene.
We improved characterization of BBa_K1172501 via NPN-Uptake assay. Fluorescence emission was measured between 380 and 520 nm. 1-N-Phenylnaphthylamine (NPN) shows maximum fluorescence at 415 nm by excitation wavelength of 355 nm. iGEM13_Bielefeld-Germany measured fluorescence emission maximum at 355 nm, which leaded to similar results but has to be attributed to reflection effects during measurement.
This assay has been used to measure cell membrane hydrophobicity.
AFM: During the last years, atomic force microscopy has been used increasingly to investigate microbial surfaces at high resolution. The technique provides three-dimensional images of the surface ultrastructure with molecular resolution, in real time, under physiological conditions, and with minimal sample preparation.
Inoculating an overnight culture (30 mL with 1 mL of pre-culture) and induce gene expression if necessary at OD600 = 1.0.
Centrifugation (5 min at 5000 g) of 2 mL overnight culture (OD600 5 ~ 1 ∙ 109 cells/mL).
Discard supernatant and wash pellet 3 times with 1 mL of ultra pure water.
Resuspend pellet in 2 mL ultra pure water (2 ∙ 109 cells).
10 minutes sonication of glass slides in acetone.
10 minutes sonication of glass slides in ethanol.
10 minutes sonication of glass slides in ultra pure water.
Dry glass slides under gentle N2 flow.
Drop 5 μL of a 1:4 dilution of prepared cells (1,25 ∙ 106 cells) to the prepared glass slides for a submonolayer coverage.
Dry glass surface with cells under gentle N2 flow.
Wash glass surface three times with ultra pure water and dry between under gentle N2 flow.
The coated glass slides were kept cool until AFM measurement.
Inoculate an overnight culture (30 mL with 1 mL of pre-culture).
Centrifugation (8 min at 3000 g) of the whole overnight culture and discard supernatant.
Resuspend pellet in equal volume of 10 mM PBS buffer.
Repeat steps 2) and 3) twice.
Mix 1 mL of washed cells with a 10 mM stock solution of NPN (1 N phenylnaphthylamine) to a final concentration of 10 µM.
Transfer 200 µL of samples to a flat bottomed black 96 well plate.
Direct fluorescence measurement with Tecan Infinite® M200 platereader.
Inside a clear, flat bottomed 96 well plate, mix 180 µL ONPG (o-nitrophenyl-b-galactopyranoside) stock solution (3 mM in 10 mM PBS buffer) with 20 µL of washed cells.
Direct absorbance measurement with Tecan Infinite® M200 platereader.
Modified protocol from Neu & Heppel, 1965.
Here you can find the used different Cell Fractioning Buffers.
MALDI-TOF MS/MS: Vera Ortseifen gave us support dealing with protein analysis by MALDI-TOF MS. After our sample preparation and tryptic digestion she measured the samples and also provided help in the data analysis.
Atomic Force Microscopy: The working group of Prof. Dr. Dario Anselmetti, with special help from Dr. Volker Walhorn, measured our self-prepared AFM glass slides and visualized the cell surface of Escherichia coli with porins by atomic force microscopy.
Hancock REW, Brinkman FSL (2002) Function of Pseudomonas porins in uptake and efflux. Annu Rev Microbiol (56):17–38.
Helander IM, Mattila-Sandholm T (2000) Permeability barrier of the gramnegative bacterial outer membrane with special reference to nisin. Int J Food Microbiol 60: 153–161.
Liu J, Qiao Y, Lu ZS, Song H, Li CM (2012) Enhance electron transfer and performance of microbial fuel cells by perforating the cell membrane. Electrochem Commun 15: 50–53.
Logan BE (2009) Exoelectrogenic bacteria that power microbial fuel cells. Nat Rev Microbiol (7): 375–381.
Loh B, Grant C, Hancock REW (1984) Use of the fluorescent-probe 1-Nphenylnaphthylamine to study the interactions of aminoglycoside antibiotics with the outer-membrane of Pseudomonas aeruginosa. Antimicrob Agents Chemother 26: 546–551.
Zhou ZX, Wei DF, Guan Y, Zheng AN, Zhong JJ (2010) Damage of Escherichia coli membrane by bactericidal agent polyhexamethylene guanidine hydrochloride: Micrographic evidences. J Appl Microbiol 108: 898–907.


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text:
Drug task force nets 12 arrests
Psimer
Lawhorn
Sloas
By Portia Williams
portiawilliams@civitasmedia.com
The FIVCO Area Drug Enforcement Task Force (FADE) collaborated with multiple law enforcement agencies to dismantle a drug ring, resulting in the arrest of 12 alleged drug dealers engaged in multiple trafficking charges in Carter County, Ky. on Tuesday.
Tuesday's drug sweep is characterized by FIVCO FADE Task Force as a 'criminal syndicate,' which is the pushing of oxycodone, heroin, and various other controlled substances onto local streets.
According to reports, on Tuesday undercover agents from FADE, and deputies from the Carter County Sheriff's Department, Grayson Police and Olive Hill Police Departments joined forces in a sweep which led to 12 arrests, tallying 26 separate charges. Authorities say the Tuesday, June 23, sting capped off a three-year investigation of a drug trafficking network which spanned across Carter County, Ky. and into other FADE surrounding areas.
Undercover police officers purchased oxycodone tablets, 10-30 milligrams on various occasions, as well as controlled substances. The average street value of the pills sold to officers averaged $50 each. Several thousand tablets were transferred during the course of the transactions.
One FADE undercover detective said children witnessed the drug trafficking activities.
"A lot of the time children were present during trafficking," the FADE undercover detective said. "Deplorable conditions."
FADE is composed of detectives from Russell, Raceland, Flatwoods, Grayson, Olive Hill and Vanceburg Police departments and Carter and Lewis County Sheriff's Departments.
According to reports, the raids were in a few different areas in Olive Hill, but police say some ring leaders of a drug organization were arrested in a neighborhood off Route 2078. Police say drug dealers were bringing in large supplies of oxycodone, heroin and other dangerous drugs — about 800 and 1,000 dosage units of oxycodone alone every two weeks. The dealers sold the drugs to undercover cops.
J.E Crisp, director of FADE, said the investigation is ongoing, and more arrests are forthcoming.
"We had the arrest information on the Web, and then the Grayson Police served another indictment warrant later, which brought our total to (12) at the time, but we just didn't have the information of that last person arrested," Crisp said. "And we are continuing to add some more names today, there are several. It is a pretty well organized crime case."
The 11 people identified by FADE were:
Gary Sloas, 49, charged with 1st degree trafficking in a controlled substance; persistent felony offender 1.
Jason Marshall, 36, of Olive Hill, charged with 1st degree trafficking in a controlled substance.
Timothy Lawhorn, 43, of Olive Hill, charged with 1st degree trafficking in a controlled substance.
Sammy Kitchen, 52, of Olive Hill, charged with 1st degree trafficking in a controlled substance; engaging in organized crime.
Greg Rose, 51, of Grayson, charged with two counts of 1st degree trafficking in a controlled substance.
Katherine Fultz Psimer, 45, of Olive Hill, charged with 1st degree trafficking in a controlled substance; engaging in organized crime.
Susan S. Callahan, 44, of Olive Hill, charged with 2nd degree trafficking in a controlled substance; three counts of theft by deception under $500 — cold checks and probation violation.
Karen S. Fields Walker, 45, of Olive Hill, charged with engaging in organized crime.
Walter Buckner, 58, of Olive Hill, charged with engaging in organized crime, two counts of first degree trafficking in a controlled substance.
John McGuire, 59, of Olive Hill, charged with engaging in organized crime; two counts of 1st degree trafficking in a controlled substance.
Steve Williams, 41, of Olive Hill, charged with three counts of 1st degree trafficking in a controlled substance; engaging in organized crime.
All of the suspects have been detained at the Carter County Detention Center.
FADE operates in Greenup, Carter and Lewis counties. To report any type of legal activity in these counties call the FADE office at 606-836-0442.
Reach Portia Williams at 740-353-3101, ext. 1929, or on Twitter @PortiaWillPDT.
Hi! A visitor to our site felt the following article might be of interest to you: Drug task force nets 12 arrests. Here is a link to that story: https://www.portsmouth-dailytimes.com/news/488/drug-task-force-nets-12-arrests


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Q: How to align labels to the inside edge of polar bar chart I am stuck making the visualization I want. I cannot yet put images so the link is below. I almost have what I want. The issue is the labels are not correctly placed.
inverted-polar-bar-demo

I would like to have the labels be rotated like they are, but have the labels' right edges aligned to just inside the outer edge of the circle.
EDIT To clarify:
The labels I used for this example are all 'testing'. With actual data, these labels will be of different length. I want to have the end of the labels moved so that they always have their last letter next to the outside edge of the circle. So in this case, all the 'g's would be next to the outside edge.
import matplotlib.pyplot as mpl
import numpy as np
import random

bgcolor = '#222222'
barcolor = '#6699cc'
bottom = 15

N = 32
Values = np.random.random(N)*10
MetricLabels = ['testing' for _ in range(1, N+1)]

# Select the radii, thetas, and widths.
Radii = -5*np.ones(N)-Values
Theta = np.linspace(0.0, 2 * np.pi, N, endpoint=False)
width = 2*np.pi/N

# Make a list of shifted thetas to place the labels at.
ThetaShifted = np.copy(Theta)
for i in range(N-1):
    ThetaShifted[i] = (Theta[i] + Theta[i+1])/2.0
ThetaShifted[-1] = (Theta[-1] + 2.0*np.pi)/2.0

# Make the figure
fig = mpl.figure()
ax = fig.add_subplot(111, projection='polar')
bars = ax.bar(Theta, Radii, width=width, bottom=bottom)

# Set the outer ring to be invisible.
ax.spines["polar"].set_visible(False)

# Set the grid line locations but set the labels to be invisible.
ax.grid(False)
ax.set_thetagrids([], visible=False)
ax.set_rgrids([3], visible=False)

# Apply colors to bars based on the settings above.
for v, bar in zip(Values, bars):
        bar.set_facecolor(barcolor)
        bar.set_edgecolor(bar.get_facecolor())

# Show the metric and value labels
for counter in range(N):
    ax.text(ThetaShifted[counter], bottom-3, MetricLabels[counter],
            horizontalalignment='center', verticalalignment='baseline',
            rotation=(counter+.5)*360/N, color=bgcolor)
    ax.text(ThetaShifted[counter], bottom+0.75, np.round(Values[counter],2),
            horizontalalignment='center', verticalalignment='center',
            color=bars[counter].get_facecolor())

# Set the background color to be a dark grey,
ax.set_axis_bgcolor(bgcolor)
fig.set_facecolor(bgcolor)

# Show the figure.
mpl.show()

A: I actually solved my issue. See image and code below. The main thing to solve it was to use the monospace font family and to use rjust to create the label strings to be fixed length and right justified from the beginning. After that, it is just a matter of choosing the correct radial location for each label which should be much easier when they are all the same number of characters.

import matplotlib.pyplot as mpl
import numpy as np
import random

bgcolor = '#222222'
barcolor = '#6699cc'
bottom = 15

N = 32
Values = np.random.random(N)*10
MetricLabels = [('A'*(4+int(8*random.random()))).rjust(10) for _ in range(1, N+1)]

# Select the radii, thetas, and widths.
Radii = -5*np.ones(N)-Values
Theta = np.linspace(0.0, 2 * np.pi, N, endpoint=False)
width = 2*np.pi/N

# Make a list of shifted thetas to place the labels at.
ThetaShifted = np.copy(Theta)
for i in range(N-1):
    ThetaShifted[i] = (Theta[i] + Theta[i+1])/2.0
ThetaShifted[-1] = (Theta[-1] + 2.0*np.pi)/2.0

# Make the figure
fig = mpl.figure()
ax = fig.add_subplot(111, projection='polar')
bars = ax.bar(Theta, Radii, width=width, bottom=bottom)

# Set the outer ring to be invisible.
ax.spines["polar"].set_visible(False)

# Set the grid line locations but set the labels to be invisible.
ax.grid(False)
ax.set_thetagrids([], visible=False)
ax.set_rgrids([3], visible=False)

# Apply colors to bars based on the settings above.
for v, bar in zip(Values, bars):
    bar.set_facecolor(barcolor)
    bar.set_edgecolor(bar.get_facecolor())

# Show the metric and value labels
for counter in range(N):
    ax.text(ThetaShifted[counter], bottom-.075*(10+len(MetricLabels[counter])), MetricLabels[counter]+' '*5,
            horizontalalignment='center', verticalalignment='center',
            rotation=(counter+.5)*360/N, color=bgcolor,
            family='monospace')
    ax.text(ThetaShifted[counter], bottom+1, np.round(Values[counter],2),
            horizontalalignment='center', verticalalignment='center',
            rotation=(counter+.5)*360/N, color=bars[counter].get_facecolor(),
            family='monospace')

# Set the background color to be a dark grey,
ax.set_axis_bgcolor(bgcolor)
fig.set_facecolor(bgcolor)

# Show the figure.
mpl.show()

A: If I correct understand what you want you have to add rotation property to the second call of counter cycle and align the text like here:
...
# Show the metric and value labels
for counter in range(N):
    ax.text(ThetaShifted[counter], bottom-3, MetricLabels[counter],
            horizontalalignment='center', verticalalignment='baseline',
            rotation=(counter+.5)*360/N, color=bgcolor)
    ax.text(ThetaShifted[counter], bottom+2.5, np.round(Values[counter],2),
            horizontalalignment='center', verticalalignment='center',
            rotation=(counter+.5)*360/N,
            color=bars[counter].get_facecolor())
 ...


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Insuret's core business product required significant functional and performance enhancements to maintain their market leading position. These activities had been commenced but were not realising the benefits Insuret needed and were taking longer than planned.
The approach recommended by Integral was to first gain an understanding of the current state of the business applications, including a review of the documentation produced to date. The Integral Project Manager established regular weekly meetings between Insuret management, the Vendor and the Project to review progress and commence planning, using standard project monitoring and control methods and tools. The consistency with which the vendor applied rigour to their management of the project varied and pressure had to be maintained throughout the project to achieve the agreed functionality to the level of quality required.
The Australian economy is powered by transport and NTI is Australia's leading insurer in that space. Integral worked with NTI to develop an advanced containerisation platform so it can remain at the forefront of the industry and respond rapidly to change.
NTI first adopted containers in 2017, leveraging the power to assist with new machine learning modelling techniques. NTI built a fit for purpose, container management platform during that period but as the value and opportunities of containers became obvious NTI needed to implement an enterprise level management platform. The NTI project team identified several key areas to focus on during their evaluation of various containerisation platforms.
After undertaking a proof-of-concept on a small group of applications, the project team found that Red Hat's OpenShift platform was the best fit and value for money. Once the NTI team decided to adopt the Red Hat OpenShift platform, the project could then move on to acquisition and eventually integrating Red Hat's OpenShift platform with existing systems.
Today, Heritage Bank has grown to become Australia's largest customer-owned bank and is one of the longest-running financial institutions in the country.
The New Payments Platform (NPP) is a major industry-wide initiative to develop a new infrastructure for Australian payments. It's a fast, flexible and data-rich payments system for Australian governments, businesses and consumers to meet their payment needs. Heritage Bank had an inflight project that had been struggling to achieve some of the broader business outcomes necessary to enable NPP.
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AUSTRALIA AND NEW ZEALAND COLLEGE OF VETERINARY SCIENCE (ANZCVS) supports the veterinary profession in Australia and New Zealand through the assessment and recognition of expertise and rewarding of excellence.
ANZCVS needed a system where examiners working remotely could enter exam results for verification, moderation and storage. Their current system had little automation in the processing and recording of exam results giving rise to the possibility of human error.
After thorough elicitation of requirements with the ANZCVS stakeholders, Integral designed a web based solution built on industry recognized tools, ensuring readily available skills in the market. The system is hosted in Microsoft Azure to allow for on demand scaling as well as simple technology upgrades without significant configuration.
ENERGEX had a low level automation system in place for their field workers that was not handling the increased quantity of data, providing sufficient accuracy, or speed of delivery to the field.
When developing a replacement system, ENERGEX wanted the new system to improve customer service levels, assist field staff in completing safer and higher performing field work, and use a smarter, integrated electronic schedule and dispatch tool (Ventyx Service Suite).
The resulting solution uses Field Force Automation (FFA) technology, implemented with the latest release of the Service Suite solution from Ventyx and incorporated the integration of a number of ENERGEX's host systems. This allows ENERGEX crews now locate faults in the network faster than ever through satellite navigation, tracking, and dispatch. The FFA computer system provides field crews with unprecedented access to electronic information about outages and customer premises from their vehicle, reducing phone calls and travel time during storms and other peak periods. The new system benefits millions of people throughout Queensland and New South Wales, providing faster, more efficient responses from ENERGEX.
Under IPA, all Councils are required to prepare and implement a planning scheme to help guide growth and change in their areas. Rapid growth in population, coupled with new legislation, prompted the Gold Coast City Council (GCCC) to plan an appropriate business solution for the future, requiring high levels of flexibility and efficiency.
The Developer Contributions Group is a specialist group in the GCCC responsible for calculating infrastructure charges on a daily basis. These calculations were manually intensive and heavily reliant on the speed, mathematical skills, and long-term concentration capabilities of employees. Integral worked with the GCCC to create a semi-automated program that helped support the human resources during this rapid growth. This solution aimed to achieve better satisfaction of legislative requirements, cost reductions associated with the implementation of the new legislation and the development of a flexible business solution that provided a framework for current systems and future processes.
Integral built an Oracle Fusion Middleware based SOA solution that integrated Oracle Policy Automation software (formerly RulesBurst) with the Council's mainframe based Asset Management System, Property Developer Contribution and Geographic Information System to provide a streamlined, semi-automated process. This integrated capability was ultimately exposed as a web based application called the PIP Charge Calculator.
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Author: Cameron Tuesley
2016 marks Integral's 15th anniversary and we are proud to be celebrating this milestone year with our wonderful clients, partners, staff and alumni.
Reflecting on what's made us last the distance, there's three things that stand out dedication to exceeding our clients' expectations and perseverance in finding better ways of solving technology problems and challenges, plus innovation which has become a real buzz word in the last few years, but for us as always really sat at the heart of how we address problems.
A bit of history: Integral started in 2001, like most start-ups – with a couple of people on a kitchen bench, which was quickly outgrown and replaced by offices in Brisbane, Sydney and Melbourne. Our client base expanded and within a few short years so did our capability, adding Project & Program Delivery, Information Management, and the Testing & Quality service lines to our base of Technical Services.
Off the back of all the hard work we've won a number of awards for our work and innovation. In fact Integral has now won an International IT award every year since 2012 and while that's something we're proud of and happy to talk about, the greater reward is knowing the positive impact of our work beyond business, out in the real world.
We've impacted thousands of people – helping university students to have a better online experience, creating systems that help people save money and the environment, enabling people to better plan their travel through access to real-time traffic information, even improving response times for crews to fix electricity outages. In our spare time we've also mentored students and contributed technology for an award-winning solar car, which now represents Australia at international events.
15 years on, it's fair to say Integral's work has been as much about people as it is the technology and that's unlikely to change over the next 15 years. We don't take for granted the contribution our valued clients, technology partners, and dedicated team members have made to our success.
So where to from here? Well it would be fair to say that in truly innovative companies, the future is never set in stone and that is certainly true for Integral. That said we have a number of new challenges that we are focused on right now, including the growth of our southern regional offices, our new product division and our growing international focus.
If you had asked me 15 years ago what would Integral look like in 2016, I would not have dared suggest that we would have close to 50 staff across three states and a multi international award winning business.
If you ask me today where we are going to be in 15 years from now, I'd be more than happy to tell you my vision for the company. However, what I'm really excited about is the journey that we are going to take to get there and the team we are going to build on the way and the impact that we are going to have on people and businesses as we head there. We're certainly not there yet, we're just getting started.
INTEGRAL NEWSLETTER
Explore our news content in these featured articles, case studies, team member profiles, event photos and Twitter feed. To subsrcibe to Integral's quarterly newsletter, please fill in the form below.
Integral's key insights from the AIIA NSW Financial Services Forum
September 27, 2016 , Featured Article
Integral announces partnership with Flexagon LLC (USA)
Integral – an employer of choice.
The Story So Far | Integral Infographic
July 8, 2016 , Featured Article
CAPABILITY STATMENTS
Integration Capability Statement
October 12, 2016 , Capability Statement
Mining, Energy and Utilities Capability Statement
October 4, 2016 , Capability Statement
Get to know: Mark Langham Project Management Consultant
January 1, 2017 , Staff Profile
Get to know: Elliott Caldwell – Principal Consultant
October 4, 2016 , Staff Profile
Get to know: Bernadette Greenock – Principal Consultant
July 8, 2016 , Staff Profile
Get to know: Jordan Howarth – Principal Consultant
March 8, 2016 , Staff Profile
Explore our news content in these featured articles, case studies, team member profiles, event photos and Twitter feed. To subsrcibe to Integral's quarterly newsletter, please fill in the form below.
Previous PostGet to know: Bernadette Greenock - Principal Consultant
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Integraltech @Integraltech_
06/10/2020 at 9:12pm
The Integral trophy collection has grown by two! After a long journey, they have finally arrived!
Congratulations once again to all the team members involved in achieving this outstanding outcome.
#redhat #innovation #partners #apac https://t.co/i0zw6V2QgP
The #partnership formed with #RedHat 8 years ago recognised the market move towards supported open source.
The power of OPEN #collaboration has led to highly strategic, long term, client relationships and new opportunities for both Integral & Red Hat.
https://t.co/47PvLkj1Dn
Integral carefully selects and maintains strong #partnerships based on #technologies that are built for lasting value whilst also enabling our clients to continuously #innovate and securely keep ahead of competition. https://t.co/KC29qsBfNw
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Western JAHJAH Foundation volunteers eager to serve
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Grange expects stronger Caribbean relationships after anti-doping forum
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Sports Big spending and big coaches can't halt Everton's sharp fall
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Tuesday Style
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2022's Best Malbecs Are From Argentina (Part 2)
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Tuesday Style The Pros and Cons of an Extended Engagement
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Editoral Code of Conduct
This Day in History - January 2
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The airing of the regular Sunday service for Calvary Episcopal Church, by KDKA in Pittsburgh on this day, 1921, marks the first time religious services are broadcasted on radio.
Today is the 2nd day of 2023. There are 363 days left in the year.
TODAY'S HIGHLIGHT
1991: Sharon Pratt is sworn in as mayor of Washington, DC, becoming the first black woman to head a city of Washington's size and prominence.
1492: The leader of the last Arab stronghold in Spain surrenders to the Spanish forces loyal to King Ferdinand II and Queen Isabella I.
1792: The first classes begin at Georgetown University in Washington, DC.
1799: Britain joins the Russian-Turkish alliance; Napoleon Bonaparte advances into Syria.
1893: The US Postal Service issues its first-ever set of commemorative stamps to honour the upcoming World's Columbian Exposition in Chicago.
1900: An open-door policy in China is announced by US Secretary of State John Hay.
1905: Japanese General Nogi receives a letter offering surrender from Russian General Stoessel, thereby formally ending the Russo-Japanese War.
Willis Carrier, on this day in 1906, receives a US patent for the world's first air conditioner..
1906: Willis Carrier receives a US patent for the world's first air conditioner.
1909: The dismissal of Yuan Shih-kai places Chinese Administration in Manchu hands.
1921: Religious services are broadcast on radio for the first time as KDKA in Pittsburgh airs the regular Sunday service of the city's Calvary Episcopal Church.
1935: Bruno Hauptmann goes on trial in Flemington, New Jersey, on charges of kidnapping and murdering the 20-month-old son of Charles and Anne Lindbergh; Hauptmann was later found guilty and executed.
1937: The English and Italians sign an agreement on the Mediterranean and in favour of maintaining Spain's independence.
1942: The Japanese capture the Philippine capital of Manila during World War II.
1955: The president of Panama, Jose Antonio Remon Cantera, is assassinated at a racetrack.
1971: Sixty-six people suffocate or are trampled to death when a crowd barrier gives way at a soccer match in Glasgow, Scotland.
1974: President Richard Nixon signs legislation requiring states to limit highway speeds to 55 miles an hour as a way of conserving gasoline in the face of an Organization of the Petroleum Exporting Countries oil embargo; the 55 mph limit is effectively phased out in 1987 and federal speed limits are abolished in 1995.
1976: The Soviet Union hardens its stance on emigration despite the 1975 Helsinki Agreement to permit freer movement of people and ideas in Europe.
1983: The musical Annie, based on the Little Orphan Annie comic strip, closes on Broadway in New York after a run of 2,377 performances.
1988: Right wing guerrillas ambush a train near Mozambique's western border, killing at least 22 people and injuring 71.
1991: Leftist rebels down a US army helicopter in El Salvador; one crew member dies from injuries sustained in the crash and two others are apparently executed by rebels.
1992: Military commanders in Croatia agree to stop fighting within 24 hours to allow for the dispatch of up to 10,000 UN peacekeepers.
1993: Government authorities arrest the second-in-command of the Popular Liberation Army, the third-largest Marxist guerrilla group in Colombia.
1994: Zapatista rebels take over San Cristobal de Las Casas in the Chiapas region of Mexico and battle government troops; at least 57 people die during the fighting.
1995: Less than 24 hours after Russian troops say they have taken Grozny, the capital of the separatist Chechnya region, rebels beat them back from the city centre.
1997: Kofi Annan arrives at UN headquarters for the first time as secretary general.
1999: Rebel forces shoot down a UN cargo plane with eight people aboard, the second UN plane to crash in Angola's highland war zone in eight days.
2001: Ships from outlying Taiwanese islands dock in ports in mainland China, making the first legal and direct crossing between the mainland and Taiwanese territory in more than 50 years.
2002: Veteran politician Eduardo Duhalde becomes Argentina's fifth president in two weeks as he takes on the burden of a US$132-billion public debt.
2003: Chinese space officials announce plans to launch a manned spacecraft in the second half of the year; the October 15 launch of the Shenzhou 5 mission carrying astronaut Yang Liwei makes China the third nation, after Russia and the United States, to do so.
2004: The summit of the seven-nation South Asian Association for Regional Cooperation agrees on a framework for a free trade zone, which would start tearing down tariffs by January 1, 2006.
2005: After the devastation wreaked by the seas, a deluge from the skies deepens the misery for tsunami-stricken areas, triggering flash floods in Sri Lanka that send evacuees fleeing and increasing the threat of deadly disease.
2006: The roof of an ice rink in Germany collapses after heavy snowfall in a town in the Bavarian Alps, killing 15 people and injuring dozens.
2007: Kenya deploys troops, armoured vehicles, and trucks with light weapons along its 420-mile-long (675-kilometre) border with Somalia following reports that Islamist militiamen fleeing fighting there are pressing on the Kenyan frontier.
2008: A mob torches a church where hundreds had sought refuge in Kenya's Rift Valley city of Eldoret and witnesses say dozens of people — including children — were burned alive or hacked to death with machetes in ethnic violence that followed Kenya's disputed election. The US Justice Department opens a full criminal investigation into the destruction of Central Intellgence Agency videotapes of the interrogation of two al-Qaeda suspects; a special prosecutor later clears the CIA's former top clandestine officer and others. Oil prices soar to US$100 a barrel for the first time.
2009: Sri Lankan forces capture the Tamil Tigers' de facto capital, Kilinochchi.
2010: A chastened President Hamid Karzai must submit new Cabinet picks after defiant Afghan lawmakers reject 17 of his 24 nominees, including a powerful warlord and the country's only woman minister.
2011: The United Nations says it will do everything it can to locate areas where human rights abuses have allegedly occurred in the Ivory Coast following disputed presidential elections.
2012: Tens of thousands of protesters jeer Hungarian leaders outside a glitzy gala to mark the country's brand new constitution, accusing the Government of exerting control over everything from the media to the economy and religion.
2013: The United Nations gives a grim new count of the human cost of Syria's civil war, saying the death toll has exceeded 60,000 in 21 months — far higher than recent estimates by anti-regime activists. Secretary of State Hillary Rodham Clinton leaves a New York hospital three days after doctors discovered a blood clot in her head.
2014: The New York Times and Guardian newspapers call for clemency for Edward Snowden, saying that the espionage worker-turned-privacy advocate should be praised rather than punished for his disclosures.
2017: A suicide bomber driving a pickup loaded with explosives strikes a bustling market in Baghdad, killing at least 36 people in an attack claimed by the Islamic State group hours after French President Francois Hollande arrives in the Iraqi capital. US House Republicans vote to gut the independent Office of Congressional Ethics but public uproar forces them to back down the next day.
On this day, 2018, the World Health Organization reveals it will classify gaming addiction as a mental health condition in its next International Classification of Diseases.
2018: WHO reveals it will classify gaming addiction as a mental health condition in its next International Classification of Diseases.
2019: Two women become the first to ever enter India's Sabarimala shrine in Kerala State, after a law change, prompting protests.
James Wolfe, English general (1727-1759); Nouri Said, Iraqi prime minister (1888-1958); Michael Tippett, English musician (1905-1998); Isaac Asimov, Russian-born US writer (1920-1992); Melville "Mel" Spence, Jamaican Olympian at 1956 and 1964 Summer Olympic Games (1936-2012); Todd Haynes, US director (1961- ); Cuba Gooding Jr, US actor (1968- ); Christy Turlington, US model (1969- ); Taye Diggs, US actor (1971- ); Ben Hardy, English actor (X-Men: Apocalypse) (1991- ); Josh Taylor, Scottish boxer (1991- )
— AP/Jamaica Observer
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