text:
Power of Images: Empathy & Sympathy
This past weekend, I drove by a billboard with a massive human toe on it. It took up about half the width of the billboard and went over the top of the rectangular sign.
It was a huge toe … with serious toenail fungus.
As you can imagine, the target of the billboard was people with toenail fungus, and the message is that there's a business that can fix it!
It may do a great job of getting attention, but it demonstrates a misunderstanding of how images affect us. A clearer understanding can make a big difference in our websites.
Many years ago, I built a site for a local nonprofit that dealt with infertility. The reason the organization even existed is that the founder dealt with personal infertility and managed to overcome it. She wanted to help others in their time of need, and raise money for couples to get medical assistance.
She could empathize, because she's been there.
At one point, we were discussing images for the homepage, and she suggested we show a smiling couple holding a newborn baby. It's a natural thing to consider, especially because that's the dream that that organization can help clients realize!
But, I asked her to imagine seeing the website with that image back before she had medical success. What would it feel like to be the type of people you want coming to your site? What would you feel if you saw that image while struggling with infertility?
She answered pretty easily with something along the lines of "defeat".
Yes, people buy outcomes and dreams and stories, but you have to help them bridge the gap between where they are and where they could be. Your story has to be believable. Jumping straight to a nearly unbelievable "happy ending" simply reinforces negative emotions about the way things are today. Going from infertility to parent is a hard road that's often literally impossible. Extend your empathy.
Conversely, if the path forward for someone is clear and believable, reinforcing an existing, negative situation isn't helpful, either. The aforementioned massive fungal toenail might catch attention, but it only works in spite of itself (if at all). A healthy toe would catch the same attention, but would likely not trigger the same negative emotions as the parenting image, because the transition is believable.
Be aware of what triggers negative feelings in your target audience, and make sure that affects your image choices. Images affect our brains in powerful ways.
There's a reason we've seen so many sad images in advertisements from nonprofits dealing with things like extreme poverty. There's a powerful, science-based explanation for why it works. The problem is it's easy to overextend, and so many nonprofits and companies use negative imagery even when it's not helpful (as in our toe fungus marketing effort).
Highly emotional images and video, which cause us to sympathize (crucial distinction), raise our levels of oxytocin. Oxytocin increases generosity and trust.
See why this is powerful? Businesses can trigger trust; nonprofits can trigger generosity. Any moral implications aside, this has been understood and employed for decades because it's been shown to work (even before we had the scientific explanation). Further, seeing another human face has psychological effects of its own that can be useful.
Again, though, the distinction is crucial: whether in "sadvertisements" or stories that pull on heart strings, the key is sympathy. While empathy is our connection to something we've actually experienced, sympathy is how we connect to something we understand, but haven't gone through ourselves. Most of us have experienced neither extreme poverty nor the type of 90-minute emotional resolutions that happen in movies.
Images can have a serious impact on your visitors and their actions, but that impact can be negative. Understanding the difference between triggering sympathy and empathy is one way you can ensure you're using visuals well.
This is post 1 of 2 in the series "The Power of Images"
Power of Images: Faces
If you have questions or comments about anything you read from us, let us know! We'll get you an answer promptly. (No sales pitch involved, we want to help.)
Written by Cliff Seal. Last Updated 6 years ago.
Topics: Content Strategy, Marketing, Small Business, Website Imagery
← Content Traps in Drag and Drop WordPress Page Builders
Power of Images: Faces →
Managing Your Own Site?
We've written a whole series to help you understand the scope of WordPress management and avoid disruptive issues.
Get ramped up on the fundamentals of WordPress management with the most critical pieces of information, allowing you to make smart purchasing decisions.
If Website Concierge sounds like a good fit for your organization, let's chat! Tell us more about yourself and we'll be in touch promptly.
Copyright © 2023 Logos Creative | Website Concierge is a Logos Creative service
About Us | Contact Us | Terms of Service | Privacy Policy


text:
Clobazam (nomes comerciais: Urbanil, Castilium, entre outros) é uma substância utilizada como medicamento pertencente ao grupo e sub-grupos:
Medicamentos Sistema nervoso central
Psicofármacos
Ansiolíticos,  sedativos e hipnóticos
Benzodiazepinas

Indicações
Perturbações da  ansiedade e sintomas ansiosos
Também é usado no tratamento de epilepsia, associado com medicamentos específicos antiepilépticos

Reacções adversas
Sonolência
Descoordenação motora
Alterações gastro-intestinais
Diarreia
Vómitos
Alterações do  apetite
Alterações visão
Irregularidades cardiovasculares
Amnésia anterógrada
Confusão
Depressão
Vertigem
O seu uso prolongado pode causar dependência e síndrome de abstinência quando a medicação é interrompida

Contra indicações e precauções
As doses devem ser reduzidas nos idosos
Deve ser administrado com cuidado em doentes com miastenia gravis ou insuficiência respiratória ou com apneia do sono
Clobazam deve ser evitado em doentes com porfiria

Interacções
Deve ser evitado o uso concomitante de álcool e medicamentos depressores do Sistema Nervoso Central

Posologia
Em crianças com mais de três anos de idade a dose a administrar nunca deve ultrapassar metade da dose indicada para adulto.

Farmacocinética
Clobazam atravessa a barreira placentária e aparece em pequenas doses no leite materno
É absorvido no trato gastro-intestinal.
Atinge o seu pico máximo de concentração no plasma entre uma a quatro horas após a administração
Cerca de 85 % do Clobazam, liga-se às Proteínas plasmáticas
A sua metabolização é efectuada no fígado, formando-se dois metabolitos: o 4-hidroxiclobazam e o N-dimetilclobazam
O Clobazam tem uma semi-vida de dezoito horas e o seu metabolito N-dimetilclobazam tem uma semi vida de quarenta e duas horas
O clobazam atravessa a Barreira hematoencefálica

Excreção
Clobazam é excretado pela urina, assim como os seus dois metabolitos

Classificação
MSRM
ATC - N05BA09
CAS - 22316-47-8

Fórmula molecular
 C16H13ClN2O2

Ver também
Benzodiazepina
Epilepsia

Clobazam
Ansiolíticos
Organoclorados
Moduladores alostéricos positivos ao receptor GABAA

redpajama_set_name: RedPajamaWikipedia


 Interesting Facts about Animals | Англійська мова з Liuro!
Animals flooded our planet. They are everywhere: some are in the deepest hollows of the ocean, while others fly over the highest points of the Earth. There are others that have never been on the ground and did not see the sun. And of course some of them live with us Nature is diverse.
But do we know much about our smaller friends? What secrets do they have? What interesting facts about animals we did not learn at school?
I believe that our article "Interesting Facts about Animals" will give you not only interesting, useful and sometimes even funny information but also it helps you in learning English.
A pig can't look up into the sky.
A horse and a rat can't vomit.
A crocodile cannot chew and can't even move its tongue.
Ostriches can run faster than horses and can roar like lions.
A sloth takes two weeks to digest its food.
A shark and a ray are the only animals that don't get cancer.
A skunk can accurately spray their smelly fluid as far as ten feet.
A dog has better eyesight than a human, but not so colorful.
North American oysters make pearls of zero value.
A human birth control pill works on gorilla.
There is a biological reserve for golden toads because they are rare.
The odds of seeing three albino deer at once are 1 in 79 billion.
Cats have scent glands in their faces.
Cats sleep up to 18 hours a day, but they often wake up to check that their environment is still safe.
A beetle tastes like an apple, a wasp like a pine nut, and a worm like fried bacon.
A cockroach can live several weeks without its head.
An elephant is the only one of mammal that can't jump.
A hedgehog's heart beats 300 times a minute.
More people are killed from bees than from snakes.
A camel has 3 eyelids for protection from blowing sand.
The placement of a donkey's eyes helps it to see all four feet at all the time!
Some worms eat themselves when they can't find food.
A dolphin sleeps with one eye open.
The longest flight of a chicken was 13 seconds.
A slug has 4 noses.
A giraffe cleans its ears with its 21-inch tongue!
An ostrich's eye is bigger than its own brain.
A butterfly can taste with its feet.
A polar bear is left-handed.
A cow can has regional accents.
A cat can make a black bear to climb a tree.
A polar bear can win the fight with a lion.
A bull is color blind.
A group of twelve or more cows is called "a flink".
A group of kangaroos is "a mob".
A group of owls is "a parliament".
A group of ravens is "a murder".
A group of bears is "a sleuth".
A baby oyster is "a spat".
A male rabbit is called "bucks", a female is "does".
An elephant can be pregnant to 2 years.
The Poison Arrow frog can kill 2,200 people.
An Emu can't walk backwards.
A chicken can't swallow while it is upside down.
A goldfish has a memory span of 3 seconds.
A mule can't sink in quicksand but a donkey can.
A penguin is the only bird that can leap into the air.
A Pig can run a mile in 7.5 minutes.
A squid has 10 tentacles.
If a horned toad is angry, it can squirt blood from its eyes.
A scallop has 35 blue eyes.
There is only one dog that doesn't have a pink tongue – it is the chow.
A zebra can't see the color orange.
You can lead a cow upstairs but not downstairs.
A gorilla can catch human colds.
A newborn Chinese water deer can fit in the palm of the hand.
A lion in the wild makes no more than 20 kills a year.
Half the pigs in the world are in China.
A deer has no gall bladders.
There are over 50,000 spiders per acre in green areas.
Bats are the only mammal that can fly, but can't walk.
A tarantula spider can live more than 2 years without food.
If you lift a Kangaroo's tail off the ground it can't hop.
If you keep a goldfish in a dark room, it will become pale.
An alligator can live up to 100 years.
One elephant teeth can weigh as much as 9 pounds.
During World War II, Americans trained bats to drop bombs.
The flamingo eats only when its head is down.
An ant never sleeps. Also it doesn't have lungs.
A cow has four stomachs.
An anteater is 6 feet long, but its mouth is only an inch wide.
A herd of 60 cows can produce more than a ton of milk a day.
There is a butterfly in Africa which poison can kill six cats.
A black panther is really a black leopard.
A tiger has striped skin like their fur.
A hippo can run faster than a human.
There are 8 different kinds of tree kangaroos.
A woodpecker pecks 20 times per second.
An elephant weighs less than the tongue of a blue whale.
A moth has no stomach.
An elephant can smell water from 3 miles away.
The stone fish is the most poisonous fish in the world.
A killer whale is not a whale at all, it is a species of dolphin.
A whale's heart beats 9 times a minute.
An elephant is covered with hair.
A firefly is the only creatures that give off light without generating heat.
A hummingbird beats their wings 60 to 80 times per second.
An elephant drinks 200 liters of water per day.
A squirrel cannot see the color red.
A chicken lays about 260 eggs per year.
A starving mouse can eat its own tail.
A butterfly has 12,000 eyes.
In the Caribbean there is oyster that can climb trees.
A giraffe has no vocal cords.
The Starfish has 8 eyes – 1 at the end of each leg.
A cat can hear ultrasound.
The tuna fish can swim 40 miles a day.
A penguin can jump 6 feet out of water.
A blue whale has large enough aortas for a human to crawl through.
A polar bear is largest land predator.
A butterfly can only see the colors red, green and yellow.
There is only one pink bottlenose dolphin. It is on an inland lake in Louisiana, USA.
A frog doesn't drink water. It absorbs water through its skin.
A crocodile has brains no larger than a cigar.
Копіювання матеріалу заборонено. © 2012 «Liuro».
Друзі, ми завжди намагаємось максимально якісно подавати матеріали на нашому сайті, і маємо надію, що в нас це виходить. Приділить секунду свого часу, та оцініть нашу роботу, щоб ми могли побачити Вашу думку. Якщо у Вас є зауваження або пропозиції стосовно статті, залиште їх в коментарях.
Ваша імейл адреса не буде опублікована.
Більше 13 000 людей долучились до Liuro в соціальних мережах. Долучайтеся і ви!
Використання інформації з даного сайту можливе лише з письмового дозволу компанії ТОВ "СтрітСтайл".


text:
Maramonovca este un sat din raionul Drochia, Republica Moldova. Majoritatea populației este de origine etnică ucraineană, stabilită aici după ocuparea Basarabiei în 1812. Originari din nordul Maramureșului istoric (de la care vine și numele) au creat o așezare de mari dimensiuni, temporar numită Moara Nouă în perioada interbelică.

Note

Localități din raionul Drochia
Colonii evreiești din Basarabia


President Obama held a press conference to give details about his pending Executive Actions for gun control. Executive Actions in and of themselves are not legally binding. They are generally regarded as a "Wish List".
The ATF will expand the definition of who is a firearms dealer, regardless of where the firearms transaction takes place or how many transactions are completed.
There is no specific threshold number of firearms purchased or sold that triggers the licensure requirement. But it is important to note that even a few transactions, when combined with other evidence, can be sufficient to establish that a person is "engaged in the business."
This will increase the number of NICS background checks because a background check is required for any firearms transaction between a firearms dealer and the buyer. Taken to the extreme, all firearms transactions public or private will require a background check.
Change the requirements for background checks regarding NFA trusts.
The National Firearms Act imposes restrictions on sales of some of the most dangerous weapons, such as machine guns and sawed-off shotguns. But because of outdated regulations, individuals have been able to avoid the background check requirement by applying to acquire these firearms and other items through trusts, corporations, and other legal entities.
Many gun owners create trusts to place NFA items in for many reasons. Now, the owner(s) of the trust will have to be fingerprinted and photographed.
Require States to release more information of their citizens to NICS.
…the Attorney General has written a letter to States highlighting the importance of receiving complete criminal history records and criminal dispositions, information on persons disqualified for mental health reasons, and qualifying crimes of domestic violence.
FBI will hire more than 230 additional NICS examiners and other staff members to assist with processing mandatory background checks.
FBI has partnered with the U.S. Digital Service (USDS) to modernize NICS. Although NICS has been routinely upgraded since its launch in 1998, the FBI is committed to making the system more efficient and effective, so that as many background checks as possible are fully processed within the three-day period before a dealer can legally sell a gun even if a background check is not complete.
The President's budget for FY2017 will include funding for 200 new ATF agents and investigators who can help enforce our gun laws, including the measures announced today.
ATF is dedicating $4 million and additional personnel to enhance the National Integrated Ballistics Information Network (NIBIN).
ATF has established an Internet Investigations Center (IIC) staffed with federal agents, legal counsel, and investigators to track illegal online firearms trafficking and to provide actionable intelligence to agents in the field.
Under current law, federal firearms dealers and other licensees must report when a gun from their inventory has been lost or stolen. The regulations are ambiguous, however, about who has this responsibility when a gun is lost or stolen in transit.
…the Attorney General is issuing a memo directing U.S. Attorney's Offices around the country to engage in renewed efforts to coordinate with these groups to help combat domestic violence and to prevent prohibited persons from obtaining firearms.
…the Administration is proposing a new $500 million investment to help engage individuals with serious mental illness in care, improve access to care by increasing service capacity and the behavioral health workforce, and ensure that behavioral health care systems work for everyone.
The Social Security Administration (SSA) has indicated that it will begin the rulemaking process to ensure that appropriate information in its records is reported to NICS.
The rulemaking will also provide a mechanism for people to seek relief from the federal prohibition on possessing a firearm for reasons related to mental health.
Some State officials raised concerns about whether such reporting would be precluded by the Privacy Rule issued under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Today, the Department of Health and Human Services issued a final rule expressly permitting certain HIPAA covered entities to provide to the NICS limited demographic and other necessary information about these individuals.
Increase research and development efforts.
Promote the use and acquisition of new technology.


Rogers Stirk Harbour + Partners were appointed to redevelop a historic bullring (built in 1898) in the city of Barcelona into a major new mixed leisure and entertainment complex. The main aim of the redevelopment is to retain and refurbish the striking mudejar façade. Inside will be approximately 45,000 square metres of mixed activities and a 1,200 space car park in the basement.
The roof with 76 m diameter and the giant dish are supported on huge pylons and rises only ten metres from its perimeter to the centre. The proposed grid-shell of timber will not only provide a visual contrast with the steel used to construct the 100 metre-diameter dish, but has the environmental advantage of being a sustainable and renewable natural resource. The choice of timber also meant that the structure could be exposed, to dramatic visual effect, as fire performance is achieved by sacrificing charring layers.


text:
Q: Random uneditable node I have come across this problem in the past but never solved it.
I am on the verge of finishing a Drupal site for a client. I created two content types and gave him the permissions necessary to edit any occurrence of these node types.
The strange problem is the client can edit any node of type product_type except for one particular node of the same type.
So in other words, all nodes of type product_type are editable except node/3.

A: Do you have a node access module installed, like Content Access or Taxonomy Access Control? If you do, it could be that there are per-node permissions set and your client's role doesn't have permission to edit that node, or it could be that it is tagged with a particular taxonomy term which prevents him from editing it.
If you don't have any extra node access modules installed, is it just that the link to "Edit" is missing in the tabs section of the page? Can the client type in "http://example.com/node/3/edit" and reach the edit screen that way?
If you supply an answer to these questions in the comments, I'll edit this answer as best I can.

A: Check the author of the node. If it's different than the client's user account that may account for the problem. If that does not work you may need to "rebuild permissions."

A: I found out what the issue was. The client account could not edit anything with full-html input format.
I enabled that at /admin/settings/filters and it works just fine now.
Thanks for looking in.

redpajama_set_name: RedPajamaStackExchange


Buying Vs. Renting - Augusta Financial, Inc. :Augusta Financial, Inc.
If there is any time to buy it is NOW! Why? Because home prices are low today. Low home values are surely not good for people selling homes but they are great news for people wanting to buy a home. Don't miss this opportunity to take advantage of the current market before home values rise.


Peter Frankl (born 2 October 1935) is a Hungarian-born British pianist. He mainly performs music from the Classical period (particularly Mozart), the Romantic period and the early Modern period. His recordings include the complete solo piano music of both Debussy and Schumann.
After studying at the Franz Liszt Academy of Music in Budapest, Frankl won several piano competitions in the late 1950s. He made his London concert debut in 1962 and first performed in New York in 1967 when he appeared with the Cleveland Orchestra under George Szell. He also studied with Maria Curcio, the last and favourite pupil of Artur Schnabel. Since then he has appeared as soloist with many other orchestras and conductors. He has been a guest at many international festivals, for example performing the Britten piano concerto with the composer conducting at the Edinburgh International Festival.
Frankl's repertoire also includes chamber music. Eleanor Warren of the BBC formed a long-term collaboration between Frankl and his compatriot violinist György Pauk and the American cellist Ralph Kirshbaum. The BBC later commissioned Fourteen Little Pictures by James MacMillan to mark the 25th anniversary of their trio in 1997.


3 Jun 2014 7:25:13 pm Congrats on Level 100! Round 17!
11 Feb 2013 2:58:27 pm Well done,level 100 R14!
26 May 2012 1:19:57 pm Congrats on 311 round 12! :)!!!!
17 Apr 2012 7:42:24 am Yay you over level 200 now! CONGRATS!!!!
22 Mar 2012 4:10:03 pm first to slay the titan round 12, well done!


text:
---
layout: default
comments: true
---

{{ page.title }}

{{ page.date | date: "%b %-d, %Y" }}{% if page.author %} • {{ page.author }}{% endif %}{% if page.meta %} • {{ page.meta }}{% endif %}

    {{ content }}

      {% include sharePage.html %}

      {% if page.comments %}

        Please enable JavaScript to view the comments powered by Disqus.
        comments powered by Disqus
      {% endif %}

              {% if page.previous.url %}

                  ← Previous Post

              {% endif %}
              {% if page.next.url %}

                  Next Post →

              {% endif %}

redpajama_set_name: RedPajamaGithub


Institute for Strategic Analysis Risk (ISAR) and www.risk-academy.ru present artificial intelligence chat-bot that helps non-financial companies and government agencies evaluate risk management effectiveness, determine risk management maturity level and develop a set of recommendations for integrating risk management into decision making and core business processes.
This is an amazing tool that can help companies quickly and efficiently determine their level of risk management maturity. And it's completely free.
Chat-bot is available to Telegram users.
ISAR CHAT-BOT allows risk managers to conduct self-assessments testing current level of risk management maturity and its effectiveness in line with the ISO31000 principles as well as international best practices.
The assessment is focused on the level of risk management integration into business processes, decision making and the overall culture of the organization.
develop a road map to optimize risk management and integrate into decision making and key business processes.
We guarantee confidentiality of the information.
ISAR CHAT-BOT developed by Vitaly Kirweny, Konstantyn Dozhdykov, Alex Sidorenko and is available in English and Russian.


text:
Gina Scrofano
Lawsuit Filed Against Laundry's Inc. For Cruel Treatment of Tigers and Violation of the ESA
(Tiger Downtown Aquarium - Houston, TX)
For over 13 years, four tigers, Nero, Marina, Coral and Reef, have been kept indoors at the Downtown Aquarium, owned by Landry's Inc.Named "Maharaja's Temple," the tiger exhibit has cement and stone flooring, insufficient sunlight, lacks adequate fresh air and water, and is a mere 100 square feet, in comparison to the 40 square miles tigers can range, and the 18 miles they can swim up to per day in the wild. These inadequate living conditions deny the tiger's of their most natural behaviors, such as swimming, running, stalking, or simply breathing fresh air, feeling the warmth of the sun or grass on their feet.
Such deprivation is in violation of the Endangered Species Act (ESA) of which has been protecting tigers for over 47 years, and the Animal Legal Defense Fund (ALDF) is not standing for it.
In September of 2017, the ALDF served Landy's Inc. with a notice of intent to sue, while according to the ALDF, also "...offering to forego the lawsuit if the company accepted the group's offer to rehome the tigers to a reputable sanctuary at no cost to Landry's."
However, in an attempt to tighten their self-serving and oppressive grip on the tigers, Landry's Inc. and Downtown Aquarium Inc. filed a lawsuit against the ALDF for defamation; claiming the ALDF was publicly and falsely harming their reputation.
On February 24th, a Harris County Judge granted the ALDF an anti-SLAPP motion (anti-strategic lawsuit against public participation) and dismissed Landry's suit. Simply put, the judge ruled that Landy's defamation suit was completely baseless and that the ALDF, along with the public, have the right of free speech.
The judge also awarded the ALDF with over $600,000 in sanctions and attorneys' fees against Landry's. As promised, the ALDF has now taken the next step and filed a lawsuit against Landy's for the mistreatment of Nero, Marina, Coral and Reef, and violation of the ESA.
The Downtown Aquarium claims on their website that the tiger exhibit is accredited by the Association of Zoos & Aquariums (AZA) and the United States Department of Agriculture (USDA), "which hold the property to the highest of industry standards." They also claim that "fewer than 10 percent of all USDA public display permit holders are able to meet AZA's rigorous accreditation standards," and "routine inspections by USDA are conducted annually and the property remains in total compliance."
Whether or not the USDA considers the inadequate living conditions of the animals held by Landy's and Downtown Aquarium in compliance with US animal welfare regulations and the ESA is a story for another day.
The fact of the matter is, out of over 100 facilities that are AZA accredited, only two of them that house tigers in the US do not provide outdoor access for the animals, and both of them are owned by Landry's; Downtown Aquarium in Houston and Downtown Aquarium in Denver. Downtown Aquarium in Denver also holds Sumatran Tigers, and both locations display other animals such as sea otters, turtles, freshwater and Cownose rays, as well sharks, a verity of other fish, and amphibians.
NH GOP Prevents Animal Protection Bills With House Vote To End Session
NH House Committee Votes to Protect Wildlife From Cruelty, and Dogs From Theft, Poison
Enhancing NH Animal Cruelty Law, HB 1560-FN
About Straight Twist
Go to FAQ to learn more about Straight Twist and how to help safeguard animals.
© 2021 StraightTwist
Please feel free to contact Straight Twist with any questions that you may have.
Info@StraightTwist.com

redpajama_set_name: RedPajamaCommonCrawl


Here are a few kind words about my coaching services from some of my happy and successful clients.
Please note: while the majority of my clients are women, I do work with many awesome men too!
Want to be my next coaching success story? Click here to apply!


If you see one of our products cheaper somewhere else, call us immediately on 1890 989 353 and we'll double the difference!
We will do the rest. We look forward to hearing from you!
We promise to match prices against any other Irish based retailer.
The www.beechfieldhealthcare.ie Price Match Promise is valid at the time of purchase and depends on a valid offer online.
The competitor's item must be an identical model to that advertised on www.beechfieldhealthcare.ie.
The product must be in stock and available for delivery within 3 to 5 days.
You must provide a URL to the identical model on our competitor's website.
The price match promise offer does not apply to free items, multiple/bundle offers, negotiated prices/quotes, clearance items or limited stock or purchases made on finance.
The competitor's price must include delivery charges. If not, the delivery costs will be added to the offer price to create a total price inclusive of delivery. Delivery charges will be estimated using the competitor's delivery policies based on that product alone being ordered.
We must be able to verify the competitor's price online ourselves.
Product is being offered on an Irish website.
Your delivery address must be within Ireland and your chosen product must be delivered to that address.
No other promotional offers can be used with a product which is matched e.g. discount vouchers cannot be applied.
We cannot retrospectively Price Match after you've ordered a product.
Our delivery charges are added onto the total order price and are not included in the price of an individual item. The delivery charges are dependent on the size and weight of the items within your final order, the delivery destination and your chosen delivery method (e.g. Next Day, Specific Day, Engineered).
We reserve the right to restrict the number of items that can be purchased at a matched price to that which can be reasonably expected to be for personal use.
Purchase of multiple items at a matched price will only be allowed at the discretion of the Beechfield customer service team.


The Mindy Project Review: "Road Trip"
This episode of The Mindy Project was more like The Danny Project. Mindy was literally on the screen for less than 3 minutes while Danny and Morgan went on a cross-country road trip to California. While the episode has funny moments, without the whole cast, it is seriously lacking. The whole time I watched, I was hoping they would flash back to the crew at Shulman and Associates, but it never happened. Instead, there was one scene with Mindy and Danny on the phone for one minute. Bottom line: Bring back the whole cast. Keri is a professional chatterbox who loves watching TV & movies, reading about pop culture, and gawking at any craziness on the internet. You can follow Keri on Twitter.


Organic Lemongrass Tea Bags – Atman Tea Co.
This tea is made with premium quality Organic Lemongrass, which brews up into a delightful, lemony-flavored tea. Containing antioxidants and healthful properties, lemongrass is cooling in nature and makes a calming cup of tea to help sooth, relax and revitalize the mind and body. You can sweeten it with honey or stevia, and even chill it and serve it cold.


The best way to live with art is not to be precious about it. So when Glenn and Sonja Hawkins briefed architect Andrew Patterson to design their new Auckland home, they didn't talk much about their art collection, or where particular works might hang. Instead, they wanted a home that would allow them and their four children – two of whom are away at university, but regularly boomeranging back – to live with ease and comfort. All they asked for was light and sun, and for the home to have a strong connection with its garden. The artworks – which include pieces by Bill Culbert, Francis Upritchard, Max Gimblett, Karl Maughan and many more – would find ways to fit in when everything else was finished.
Patterson responded with a "pavilion in a garden" inspired by the symmetry and severity of late 1960s Italian modernist buildings. He had spied these structures in city-fringe industrial areas during visits to Milan, and their discipline and rigour appealed to him just as much as the way they were often clad in repetitive arrangements of prefabricated panels. He also keyed into the language of the former building on the site, a group of four 1960s flats that possessed their own blunt modernist style. From the exterior, the building's steel frame sets up a pattern in which windows alternate with panels of glass-reinforced concrete. It is a home that looks simultaneously classical and contemporary.
These days, many new family homes seem to be designed with the intention of their occupants living almost separate lives. This one works differently, with a large combined kitchen, dining and living area serving as the home's hub (there is a small TV room nearby, but there are no zones designated for the sole use of adults or children). The glassy multi-purpose living area is anchored by a large, steel-clad, double-sided fireplace that also serves the terrace that extends off the space and overlooks the garden outside. When he was creating it, Patterson envisaged a gracious rural home that just happened to exist in the city. The bedrooms upstairs, for example, are linked by a wide hall with windows at both ends that feel like a contemporary take on a colonial tradition.
Sonja, an interior designer, chose an elegant backdrop of robust materials indoors: oak boards for the walls, travertine floors and flowing linen drapes. In the kitchen, which has windows looking into a large butler's pantry, she selected a black granite bench and devised an open shelving system from brass pipes that complements the tapware. All of it is pared-back, unfussy and timeless.
The living area's glassiness doesn't really allow for the display of art, but Glenn and Sonja say they didn't set out to build a gallery. Their interest in art is not focused on building their own collection, but on bringing the joy and sustenance of art to a wider range of people. Glenn, the founder of Avanti Finance, is a trustee of the Auckland Art Gallery Foundation, which was established to raise money for the gallery's new building, and is now a vehicle to grow an endowment fund for the gallery's ambitions, from creating new exhibitions to developing larger educational initiatives for schools. The main challenge, Glenn says, is to break the perception that philanthropy is something that only happens when donors have tens of thousands of dollars to give, when contributions of any size can support the foundation's mission to make art available to the widest possible audience. "If somebody can give $50, that's fantastic," he says.
Last year, the couple took another step towards supporting artists' development by establishing MyArt, a service that provides interest-free loans to art buyers who purchase works from accredited galleries. The idea is that the loans will make it easier for people to take the leap and purchase an artwork, something the Hawkins hope will increase turnover in the art market as a whole, and therefore mean more artists are able to earn enough to support themselves. A similar service in the UK has proven highly successful: 25 percent of its customers are first-time art buyers, and 80 percent reported that the availability of loans meant they increased their art spend.
All this philanthropic activity happens from a beautifully designed home base. In many senses, Sonja and Glenn's love of art and respect for the artistic process made them ideal architectural clients, too. "Andrew is the sort of guy who, if he has a bit of an open brief, gets the juices flowing," Glenn says. You can see the results in the rigour and simplicity of the place they now call home. "It's a severe building, but it's designed for a beautiful life," Patterson says.
Words by: Jeremy Hansen. Photos by: Simon Devitt.


text:
Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil
Dhiraj Kumar Chaudhary ORCID: orcid.org/0000-0003-4845-39331,
Dong-Uk Kim2,
Dockyu Kim ORCID: orcid.org/0000-0001-7504-62473 &
Jaisoo Kim1
Scientific Reports volume 9, Article number: 4134 (2019) Cite this article
This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42T and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that these Arctic strains belonged to the genus Flavobacterium, with the nearest relative being Flavobacterium psychrolimnae LMG 22018T (98.9% sequence similarity). The pairwise 16S rRNA gene sequence identity between strains Kopri-42T and Kopri-43 was 99.7%. The DNA-DNA hybridization value between strain Kopri-42T and Kopri-43 was 88.6 ± 2.1% indicating that Kopri-42T and Kopri-43 represents two strains of the same genomospecies. The average nucleotide identity and in silico DNA-DNA hybridization values between strain Kopri-42T and nearest relative F. psychrolimnae LMG 22018T were 92.4% and 47.9%, respectively. These values support the authenticity of the novel species and confirmed the strain Kopri-42T belonged to the genus Flavobacterium as a new member. The morphological, physiological, biochemical and chemotaxonomic data also distinguished strain Kopri-42T from its closest phylogenetic neighbors. Based on the polyphasic data, strains Kopri-42T and Kopri-43 represents a single novel species of the genus Flavobacterium, for which the name Flavobacterium petrolei sp. nov. is proposed. The type strain is Kopri-42T (=KEMB 9005-710T = KACC 19625T = NBRC 113374T).
Anthropological activities have increased the level of oil-based contaminants in Arctic and Antarctica regions1. Psychrophilic bacteria have been implemented to remediate these hazardous oil-based pollutants from coldest regions of the Earth. Native psychrotrophs with degradation capabilities can be employed for xenobiotic transformation and bioremediation of petroleum hydrocarbons from Polar regions2. As a remediating agent, several strains of the genus Flavobacterium have been also used in the field of environmental bioremediation3,4,5.
The genus Flavobacterium belonging to the family Flavobacteriaceae of the phylum Bacteroidetes was first established by Bergey et al.6 and later its emended description was provided by Bernardet et al.7. During manuscript preparation, this genus accommodates 208 species with valid names (http://www.bacterio.net/flavobacterium.html). The members of the genus Flavobacterium comprises diverse habitat and have been isolated from compost materials, diseased fish, algal mat, marine environments, rhizospheric niches, toxic circumstances (including petroleum products-contaminated soil), and psychrophilic regions (glacier, Antarctic lake, Tibetan Plateau, and Arctic soil)5,6,7,8,9,10,11,12,13,14,15. During the search of psychrophilic degraders of petroleum products, strains Kopri-42T and Kopri-43 were isolated from oil-contaminated Arctic soil. This study presents detail taxonomic investigation of strains Kopri-42T and Kopri-43 and briefly illustrated their diesel-degrading capacity. On the basis of polyphasic taxonomic results, both the strains are proposed as novel members of the genus Flavobacterium, with Kopri-42T as the type strain.
The nearly complete length of 16S rRNA gene sequences of the strains Kopri-42T and Kopri-43 were 1,444 and 1,438 bps, respectively. The comparative analysis of 16S rRNA gene sequence using the EZBioCloud server revealed that the strains Kopri-42T and Kopri-43 belong to the genus Flavobacterium and shared highest sequence similarity with F. psychrolimnae LMG 22018T (98.9% and 99.3%, respectively). The pairwise 16S rRNA gene sequence identity between strains Kopri-42T and Kopri-43 was found to be 99.7%. The high 16S rRNA gene sequence similarity between strains Kopri-42T and Kopri-43 revealed that both the strains could be considered as a single novel species. In addition, DNA-DNA hybridization (DDH) values between strain Kopri-42T and Kopri-43 was 88.6 ± 2.1% (reciprocal, 83.9 ± 1.5%), indicating that Kopri-42T and Kopri-43 represents two strains of the same genomospecies16. In addition, the DNA fingerprinting obtained by REP-PCR showed several similar bands between Kopri-42T and Kopri-43 but differ with reference strains (see Fig. S1 in the supplementary material), suggesting that these two strains represent the same species. Based on the 16S rRNA gene sequence similarity, the other closest neighbors of the type strain Kopri-42T were F. limicola DSM 15094T (98.3%, sequence similarity); F. tiangeerense 0563T (97.8%, sequence similarity); and F. sinopsychrotolerans 0533T (97.7%, sequence similarity). The 16S rRNA gene sequence identities between the type strain Kopri-42T and the closest phylogenetic relatives were in the range of 98.9–97.7%, which were below the threshold value of 98.7–99.0% used for species demarcation of prokaryotes17,18. These 16S rRNA gene sequence similarities values along with average nucleotide identity and in silico DNA-DNA hybridization (described below) suggested to allocate strain Kopri-42T as a new species of the genus Flavobacterium. Furthermore, the phylogenetic trees analyses obtained from 16S rRNA gene sequence alignments by maximum-likelihood (Fig. 1); neighbor-joining (Fig. S2); and maximum-parsimony (Fig. S3) also showed that strains Kopri-42T and Kopri-43 were grouped within the genus Flavobacterium and formed a cluster which consists cold-adaptive Flavobacterium species.
Phylogenetic tree inferred with maximum likelihood algorithm based on 16S rRNA gene sequences showing the relationship between strains Kopri-42T, Kopri-43, and closely related members of the genus Flavobacterium. Filled circles indicate branch-nodes recovered by maximum-likelihood, neighbor-joining, and maximum-parsimony phylogenetic trees. The numbers at the branch-nodes represent the percentage of 1,000 bootstrap replicates; only values >50% are depicted in the tree. GenBank accession numbers for 16S rRNA gene sequences are presented in parentheses. Schleiferia thermophila TU-20T was included as an out-group. The scale bar represents 0.05 substitutions per nucleotide position.
The whole genome sequence of strain Kopri-42T contains 3738413 bp (GenBank accession number: QNVY00000000). The full genome was assembled in 22 contigs with an N50 of 693296 bp and genome coverage of 609.5x. The genome-based similarity calculated based on OrthoANIu between strain Kopri-42T and closest reference strain F. psychrolimnae LMG 22018T was 92.4%. The average nucleotide identity (ANI) values with other reference strains were below 90.0% (Table 1). These values were below the threshold ANI value of 95.0–96.0% used for delineating prokaryotic species19, suggesting strain Kopri-42T is a novel strain of the genus Flavobacterium. In addition, in silico DNA-DNA hybridization (DDH) value between strain Kopri-42T and F. psychrolimnae LMG 22018T was 47.9%. The in silico DDH values with other reference strains were below 40.0% (Table 2). These values were significantly below the cut-off value of 70%, which clearly indicated that strain Kopri-42T differs genetically from other type strains of genus Flavobacterium at the species level16,20. The G + C content of the chromosomal DNA for strains Kopri-42T and Kopri-43 were 34.8 and 35.1 mol %, respectively, which is in agreement with the range of 32.0–38.0 mol % for the genus Flavobacterim7,13.
Table 1 The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) values between strain Kopri-42T and closely related reference strains.
Table 2 Differentiating characteristics of strain Kopri-42T, Kopri-43, and related species of the genus Flavobacterium.
Both strains were Gram-stain-negative, non-motile, and aerobic. The photomicrographs obtained from transmission electron microscopy revealed that cells of strains Kopri-42T and Kopri-43 were rod-shaped without flagella (Fig. 2). The colonies of both strains on R2A agar were yellow, 1.0–2.0 mm in diameter, circular, convex, translucent, and glistening with entire margins. Strains Kopri-42T and Kopri-43 grew well on R2A, TSA, and NA; poorly grew on LB, marine agar, and veal infusion agar; and did not grow on MacConkey agar. Growth was detected at temperatures 0–25 °C, pH 6.0–10.5, and 0–1% (w/v) NaCl concentration (Table 2). The optimum temperature for strain Kopri-42T was 10–15 °C as revealed from the growth curve (Fig. S4). Based on the growth temperature data, both strains are concluded to be psychrophilic bacteria. The presence of higher amount of cellular unsaturated fatty acids may contribute to the cold adaptation of these strains (Table 3). It has been documented that presence of higher proportion of unsaturated fatty acids content in the cell membrane favors bacteria to grow at low temperature21,22. Both strains were positive for catalase test but negative for oxidase test. However, oxidase test was positive for all the reference strains considered in this study. Production of flexirubin-type pigment was absent from both strains. Hydrolyze aesculin and CM-cellulose but cannot hydrolyze Tween (40/60/80), starch, casein, DNA, and tyrosine. Strain Kopri-42T was sensitive to novobiocin, tetracycline, nalidixic acid, rifampicin, cyclohexamide, trimethoprim, sulfamethoxazole and chloramphenicol but resistant to kanamycin, neomycin, gentamycin, penicillin, ampicillin, and streptomycin. Other differentiating properties and phenotypic traits resulted from API ZYM, API 20NE, and API ID 32GN test kits of strains Kopri-42T and Kopri-43 are depicted in the digital protologue table and presented along with other nearest phylogenetic members (Tables 2 and S1).
Transmission electron photomicrographs of the cells of novel strains grown on R2A agar at 10 °C for 5 days. (a) Kopri-42T; (b) Kopri-43. Bar: 1.0 µm.
Table 3 Cellular fatty acid profile of strain Kopri-42T, Kopri-43, and related species of the genus Flavobacterium.
Both strains comprise menaquinone-6 (MK-6) (Fig. S5) as sole respiratory quinone which is typical to menaquinone system of the genus Flavobacterium8. Strains Kopri-42T and Kopri-43 contain phosphatidylethanolamine (PE) as the major polar lipid. The unidentified aminolipids (AL1-AL6) and unidentified lipids (L1–L4) were also detected in minor amount (Fig. S6a,b). The major polar lipid profile of both strains shared similar pattern with F. psychrolimnae KACC 11737T (Fig. S6c). However, some proportional difference exists in the minor polar lipid profile between strains Kopri-42T, Kopri-43 and F. psychrolimnae KACC 11737T (Fig. S6). The major cellular fatty acids present in Kopri-42T were C15:1ω6c (11.6%), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 10.1%), iso-C15:0 (9.8%), iso-C16:0 3-OH (9.2%), anteiso-C15:0 (8.0%), iso-C15:1 G (5.8%), iso-C16:1 H (5.4%), and iso-C17:0 3-OH (5.3%). The observed patterns of fatty acids are similar to closest neighbors. Despite of the comprehensive similarities, a differential pattern exhibited with minor amount of fatty acids. Both strains detected iso-C17:0 which was absent from closest members. C15:0 3-OH was present in strains Kopri-42T and Kopri-43 but not detected from F. psychrolimnae KACC 11737T and F. limicola KACC 11965T. Most of the reference strains had anteiso-C17:1ω9c and C18:1ω5c but absent from strains Kopri-42T and Kopri-43 (Table 3).
Both the strains degraded substantial amount of diesel in liquid media and soil environment. In MSM liquid media, strains Kopri-42T and Kopri-43 degraded 60.0% and 58.3% of diesel oil, respectively (Fig. 3a). Statistical analysis indicates that the rates of diesel degradation by strains Kopri-42T and Kopri-43 were not significantly different (p > 0.05). In diesel contaminated soil, the degradation rate was improved with various treatment conditions. When the diesel contaminated soil was treated with only bacterial strain Kopri-42T, the remediation efficiency was 44.8%. The degradation rate was found to be enhanced (64.8%) when the contaminated soil was treated with Kopri-42T + nutrients + biosurfactants (Figs 3b and S7). Statistical analysis showed that the rates of diesel degradation between various treatment conditions were significantly different (p < 0.05). This result indicates that strain Kopri-42T and Kopri-43 can be used as biological agent for remediating diesel oil from cold environments.
Changes in diesel concentration in different treatment conditions. (a) in MSM liquid, treated for 14 days; and (b) in the standard soil, treated for 30-days. Bar represents the standard error of the duplicate samples.
In conclusion, 16S rRNA gene sequence analysis, phylogenetic trees, and chemotaxonomic data indicate both strains clearly belong to the genus Flavobacterium. The differentiating phenotypic properties and chemotaxonomic data presented in Tables 2 and 3 preliminary distinguish the two strains as members of a new species in the genus Flavobacterium. The genome sequence characteristics, OrthoANIu and DDH values, and REP-PCR confirm that both strains represent single novel species. Furthermore, both psychrophilic strains can degrade diesel oil and able to thrive in oil-contaminated cold environments indicating their significance in the bioremediation field. Considering the above mentioned genotypic, phylogenetic, phenotypic, biochemical, and chemotaxonomic data, both strains are concluded to represent a novel species in the genus Flavobacterium, with the proposed name Flavobacterium petrolei sp. nov. The type strain is Kopri-42T (=KEMB 9005-710T = KACC 19625T = NBRC 113374T). The formal proposal of the new species name Flavobacterium petrolei sp. nov. is given in the Table S1 with the TaxonNumber TA00628 (http://imedea.uib-csic.es/dprotologue/edit_entryForm.php?form_id=10891&entry_id=628).
Bacterial isolation, growth conditions, and maintenance of strain
For isolation, soil samples were collected during August and september 2012 from different corners of the base station near the DASAN, Korean Arctic Station, N-9173 Ny-Alesund, Norway (GPS location: 78°55′30.13″N 11°55′20.21″E). The diesel oil used in this study was purchased from petroleum station (GS Caltex, Suwon, South Korea). The composition of mineral salt medium (MSM) used for isolation of oil-degrading bacteria is given in Table S2. An enrichment cultivation technique employing Transwell plate (Corning) was implemented for the isolation of psychrophilic oil-degrading bacteria as described previously9,23. Pure isolates were obtained after multiple streaking bacterial colonies on R2A (MB Cell) agar incubating at 10 °C. All the strains isolated during this study are listed in Table S3. The pure culture of strains Kopri-42T and Kopri-43 grown in R2A agar was temporarily stored at 4 °C and subcultured regularly at the interval of two weeks until the taxonomic study was completed. For long-term storage both strains were stored at −80 °C in R2A broth with 20% (v/v) glycerol and later deposited permanently in culture collection center.
Genotypic characterization and phylogenetic analysis
Two strains Kopri-42T (=KEMB 9005-710T = KACC 19625T = NBRC 113374T) and Kopri-43 (=KEMB 9005-7101 = KACC 19626 = NBRC 113411) isolated from Arctic soil were characterized in this study. Genomic DNA from strains Kopri-42T and Kopri-43 was isolated using a DNA isolation kit following the exact protocol provided with the kit (InstaGene Matrix kit, Bio-Rad, USA). The conditions of PCR amplification, primers information, purification of PCR products, and sequencing and analysis of 16S rRNA gene were performed following a previously described protocol19,24. For DNA fingerprinting, repetitive extragenic palindromic (REP) PCR was performed using BOXA1R primer (5′- CTACGGCAAGGCGACGCTGACG-3′) following the conditions as mentioned previously25. The gel-electrophoresis image of PCR product was analyzed and the dendrogram was generated using PyElph 1.4 software by UPGMA method, with a bootstrap of 100. The similarity matrix was computed using Dice coefficient26.
The nearest phylogenetic members were determined by comparing 16S rRNA gene sequences of strains Kopri-42T and Kopri-43 with the sequences available in the GenBank database (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and EZBioCloud server27. For phylogenetic analysis, multiple alignment with the sequences of closest neighbors was carried out using CLUSTAL X 2.128. Gaps between the 5′ and 3′ ends of the aligned sequences were deleted utilizing the BioEdit program29. Phylogenetic trees were inferred with the help of software package MEGA630 by using three different algorithms: the neighbor-joining31, maximum-parsimony32 and maximum-likelihood33. Kimura two-parameter model32 was used to calculate evolutionary distances and the bootstrap values were calculated based on 1000 replicates34,35.
For whole genome sequencing, genomic DNA from strain Kopri-42T was extracted using the commercial kit (Genomic DNA Purification Kit, Wizard, Promega) following the manufacturer's protocol. The whole genome sequence of strain Kopri-42T was obtained using an Illumina MiSeq sequencer and assembled utilizing an assembly toolkit software SPAdes v3.10.1 at the ChunLab (Seoul, South Korea). The genome sequence was annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP, 2013). The genomic relatedness of the novel strain Kopri-42T with the whole genome shotgun sequences of closely related species of Flavobacterium was determined based on the Average Nucleotide Identity using USEARCH software tool (OrthoANIu)36. In silico DDH was calculated using Genome-to-GenomeDistance Calculator (GGDC 2.1; http://ggdc.dsmz.de/ggdc.php) with recommended BLAST + alignment and formula 2 (identities/HSP length)20.
The G + C content of the chromosomal DNA for strains Kopri-42T and Kopri-43 was determined by real-time PCR analysis37 and also genomic G + C content for the type strain was calculated based on the whole genome sequence data. DNA-DNA hybridization (DDH) was conducted between strain Kopri-42T and Kopri-43 as described previously38. Salmon sperm was considered as the negative control and photobiotin was used as probes to label genomic DNA of strain Kopri-42T. The values of DDH were determined fluorometrically in microplate wells using a 1420 Multilabel Counter (Perkin Elmer). Additionally, for reverse hybridization DNA of strain Kopri-43 was labeled with photobiotin and used as a probe to strain Kopri-42T. All the experiments were performed in triplicate.
Selection of reference strains
Based on preliminary 16S rRNA gene sequence and the phylogenetic analysis, Flavobacterium psychrolimnae KACC 11737T, Flavobacterium limicola KACC 11965T, Flavobacterium sinopsychrotolerans JCM 16398T, and Flavobacterium tiangeerense JCM 15087T were considered for comparative morphological, physiological, biochemical, and chemotaxonomic studies. In addition to the above type strains, Flavobacterium piscis CCUG 60099T and Flavobacterium xueshanense sr22T were also selected for genomic relatedness (ANI and in silico DDH) study.
Morphological, physiological and biochemical characterization
Cellular morphologies of the strains Kopri-42T and Kopri-43 cultivated on R2A for 5 days at 10 °C were observed by transmission electron microscopy (Libra 120 EFTEM; Zeiss). Colony morphologies of both strains on R2A agar were determined using a Zoom Stereo Microscope (SZ61; Olympus Japan) after incubation at 10 °C for 5 days on TSA. Gram staining type was studied following the protocol described by Doetsch39. Cellular motility, growth ability on various commercial culture media, temperature range, pH range, NaCl tolerance, catalase, and oxidase tests, spore staining, and DNA degradation assay were determined as illustrated previously9,40. To determine psychrophilic optimum temperature, the growth curve was determined at 4, 10, 15, 20, and 25 °C by measuring growth absorbance at 600 nm using visible spectrophotometer (Biochrome Libra S4). Anaerobic growth was assessed after 14 days incubation at 10 °C using an anaerobic jar (BBL, Becton Dickinson) with Gas Generating Pouch System (GasPakTM EZ). Indole test and H2S production were evaluated in SIM medium. MR-VP broth was used to perform the MR-VP test. Hydrolysis of Tween 40, Tween 60, Tween 80, starch, gelatin, aesculin, casein, tyrosine, and CM-cellulose was performed as previously described9,41. The presence of flexirubin-type pigments was analyzed with 20% (w/v) KOH solution42. Antibiotic susceptibility test was performed on R2A plates by the paper disc method43 with the following commercial antibiotics: tetracycline (30 µg), kanamycin (30 µg), rifampicin (10 µg), nalidixic acid (30 µg), novobiocin (30 µg), neomycin (30 µg), streptomycin (10 µg),gentamycin (10 µg), ampicillin (30 µg), penicillin (10 µg), chloramphenicol (100 µg), cyclohexamide (30 µg), trimethoprim (30 µg), and sulfamethoxazole (30 µg). API ZYM, API 20NE, and API ID 32GN test kits (bioMérieux) were used to determine other enzymatic, physiological, biochemical, and assimilation properties following the manufacturer's instructions.
Chemotaxonomic characterization
The respiratory quinone and polar lipids were extracted and analyzed from freeze-dried cells following the method as presented previously44,45. The TLC chromatograms of polar lipids were sprayed with appropriate detection reagents for visualization of various spots as described previously9,45,46. The cellular fatty acids of strains Kopri-42T, Kopri-43, and reference strains were extracted after late log phase grown at 10 °C on R2A using the standard MIDI protocol (Sherlock Microbial Identification System, version 6.0B) and analyzed with a gas chromatograph (6890 Series GC System; Hewlett Packard) using the TSBA6 database of the Microbial Identification System47.
Determination of diesel degradation ability
The preliminary diesel degrading ability of strains Kopri-42T and Kopri-43 was assessed in MSM liquid media containing 3000 ppm diesel oil at 10 °C as described previously23. Furthermore, the degrading ability was analyzed in standard soil contaminated with diesel oil. The diesel-contaminated soil was prepared in the lab using sand, kaolin, peat moss, and the diesel oil at 600 mg kg−1. All the components were mixed thoroughly and stabilized for 1 month prior to conducting the experiment. The physio-chemical parameters of the prepared soil were studied after stabilization and have been presented in Table S4. The diesel degradation experiment was performed in the plastic vessel (size: 24 cm × 17 cm × 16 cm) each containing 2.5 Kg of prepared soil. Diesel-remediation was evaluated in five different treatment conditions (Table S5). The bacterial inoculums, nutrients, and biosurfactants were delivered to the experimental vessels through solution-pouring technique at the 5-day interval for 1 month. The nutrients in the respective treatment conditions were added maintaining the carbon, nitrogen, and phosphorous at the ratio of 100:10:1. All the experimental vessels were incubated at 10 °C in an incubator. After 1-month treatment, residual diesel concentration was determined using GC-FID (HP 6890, Agilent, USA) following the procedure as explained previously48.
The data of diesel degradation were subjected for statistical analysis to determine mean, standard deviation (SD), and standard error. The p- value between different treatments were calculated using two-way ANOVA in the Microsoft Office Excel 2013. The p- value less than 0.05 was used to conclude significant differences between various treatment conditions.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strains Kopri-42T and Kopri-43 are MH019220 and MH019221, respectively. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession QNVY00000000. The version described in this paper is version QNVY02000000.
van Dorst, J., Siciliano, S. D., Winsley, T., Snape, I. & Ferrari, B. C. Bacterial targets as potential indicators of diesel fuel toxicity in subantarctic soils. Appl. Environ. Microbiol. 80, 4021–33 (2014).
Yang, S. et al. Hydrocarbon degraders establish at the costs of microbial richness, abundance and keystone taxa after crude oil contamination in permafrost environments. Sci. Rep. 6, 37473 (2016).
Bayat, Z., Hassanshahian, M. & Cappello, S. Immobilization of Microbes for Bioremediation of Crude Oil Polluted. Environments: A Mini Review. Open Microbiol. J. 9, 48–54 (2015).
Robles-González, I. V., Fava, F. & Poggi-Varaldo, H. M. A review on slurry bioreactors for bioremediation of soils and sediments. Microb. Cell Fact. 7, 5 (2008).
Tamaki, H. et al. Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int. J. Syst. Evol. Microbiol. 53, 519–526 (2003).
Bergey, D. H. et al Flavobacterium gen. nov. Bergey's manual of determinative bacteriology, Williams & Wilkins, Baltimore, pp 97–117 (1923).
Bernardet, J.-F. et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Evol. Microbiol. 46, 128–148 (1996).
Bernardet, J. F. & Bowman, J. P. The genus Flavobacterium. In Bergey's manual of systematic bacteriology (eds Whitman, W. B. & Parte, A. C.) 2nd ed, Vol 1, 112–155 (Springer, 2010).
Chaudhary, D. K. & Kim, J. Flavobacterium olei sp. nov., a novel psychrotolerant bacterium isolated from oil-contaminated soil. Int. J. Syst. Evol. Microbiol. 67, 2211–2218 (2017).
Huang, F. et al. Flavobacterium qiangtangensis sp. nov., Isolated from Qiangtang Basin in Qinghai-Tibetan Plateau, China. Curr. Microbiol. 69, 234–239 (2014).
Kacagan, M., Inan, K., Belduz, A. O. & Canakci, S. Flavobacterium anatoliense sp. nov., isolated from fresh water, and emended description of Flavobacterium ceti. Int. J. Syst. Evol. Microbiol. 63, 2075–2081 (2013).
Liu, H., Lu, P. & Zhu, G. Flavobacterium cloacae sp. nov., isolated from waste water. Int. J. Syst. Evol. Microbiol. 67, 659–663 (2017).
Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int. J. Syst. Evol. Microbiol. 55, 769–772 (2005).
Xin, Y.-H. et al. Flavobacterium tiangeerense sp. nov., a cold-living bacterium isolated from a glacier. Int. J. Syst. Evol. Microbiol. 59, 2773–2777 (2009).
Xu, M. et al. Flavobacterium sinopsychrotolerans sp. nov., isolated from a glacier. Int. J. Syst. Evol. Microbiol. 61, 20–24 (2011).
Wayne, L. G. et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464 (1987).
Stackebrandt, E. & Ebers, J. Taxonomic parameters revisited: tarnished gold standards. Microbiol. Today 33, 152–155 (2006).
Kim, M., Oh, H.-S., Park, S.-C. & Chun, J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int. J. Syst. Evol. Microbiol. 64, 346–351 (2014).
Richter, M. & Rosselló-Móra, R. Shifting the genomic gold standard for the prokaryotic species definition. Proc. Natl. Acad. Sci. 106, 19126–19131 (2009).
Meier-Kolthoff, J. P., Auch, A. F., Klenk, H.-P. & Göker, M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14, 60 (2013).
Morita, R. Y. Psychrophilic bacteria. Bacteriol. Rev. 39, 144–167 (1975).
Gounot, A. M. Psychrophilic and psychrotrophic microorganisms. Experientia 42, 1192–1197 (1986).
Pham, V. H. T., Chaudhary, D. K., Jeong, S.-W. & Kim, J. Oil-degrading properties of a psychrotolerant bacterial strain, Rhodococcus sp. Y2-2, in liquid and soil media. World J. Microbiol. Biotechnol. 34, 33 (2018).
Frank, J. A. et al. Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl. Environ. Microbiol. 74, 2461–2470 (2008).
Kim, I., Kim, D.-U., Kim, N.-H. & Ka, J.-O. Isolation and characterization of fenobucarb-degrading bacteria from rice paddy soils. Biodegradation 25, 383–394 (2014).
Pavel, A. B. & Vasile, C. I. PyElph - a software tool for gel images analysis and phylogenetics. BMC Bioinformatics 13, 9 (2012).
Yoon, S.-H. et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int. J. Syst. Evol. Microbiol. 67, 1613–1617 (2017).
Larkin, M. A. et al. Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–2948 (2007).
Hall, T. A. A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98 (1999).
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Mol. Biol. Evol. 30, 2725–2729 (2013).
Saitou, N. & Nei, M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–25 (1987).
Fitch, W. M. Toward defining the course of evolution: minimum change for a specific tree topology. Syst. Zool. 20, 406–416 (1971).
Felsenstein, J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J. Mol. Evol. 17, 368–376 (1981).
Kimura, M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16, 111–120 (1980).
Felsenstein, J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791 (1985).
Yoon, S.-H., Ha, S., Lim, J., Kwon, S. & Chun, J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 110, 1281–1286 (2017).
Gonzalez, J. M. & Saiz-Jimenez, C. A fluorimetric method for the estimation of G + C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4, 770–3 (2002).
Ezaki, T., Hashimoto, Y. & Yabuuchi, E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224–229 (1989).
Doetsch, R. N. Determinative methods of light microscopy. In Manual ofMethods for General Bacteriology (ed. Gerhardt, P.) 21–33 (American Society for Microbiology, 1981).
Breznak, J. A. & Costilow, R. N. Physicochemical factors in growth. In Methods for General and Molecular Microbiology (ed. Beveridge, T. J., Breznak, J. A., Marzluf, G. A, Schmidt, T. M, Snyder, L. R.) 3rd edition, 309–329 (American Society of Microbiology, 2007).
Hemraj, V., Diksha, S. & Avneet, G. A review on commonly used biochemical test for bacteria. Innovare J. Life Sci. 1, 1–7 (2013).
Reichenbach, H. The order Cytophagales. In The Prokaryotes (ed. Balows, A., Trüper, H. G., Dworkin, M., Harder, W., Schleifer, K. H.) 2nd edition, vol. 4, 3631–3675 (Springer, New York, 1992).
Brown, D. F. & Kothari, D. Comparison of tablets and paper discs for antibiotic sensitivity testing. J. Clin. Pathol. 28, 983–8 (1975).
Collins, M. D. & Jones, D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol. Rev. 45, 316–354 (1981).
Minnikin, D. E. et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J. Microbiol. Methods 2, 233–241 (1984).
Komagata, K. & Suzuki, K.-I. 4 Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol. 19, 161–207 (1987).
Sasser, M. Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. (MIDI Inc, 1990).
Bajagain, R., Park, Y. & Jeong, S.-W. Feasibility of oxidation-biodegradation serial foam spraying for total petroleum hydrocarbon removal without soil disturbance. Sci. Total Environ. 626, 1236–1242 (2018).
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1A09916982). We acknowledge The DASAN, Korean Arctic Station (Korea Polar Research Institute) for providing Arctic oil-contaminated soil. We greatly appreciate Professor Bernhard Schink (University of Konstanz, Konstanz, Germany) for providing the species name.
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea
Dhiraj Kumar Chaudhary
& Jaisoo Kim
Division of Bio-convergence, College of Convergence and Integrated Science, Kyonggi University, Suwon, Gyeonggi-Do, 16227, South Korea
Dong-Uk Kim
Division of Polar Life Sciences, Polar Research Institute, Incheon, 21990, South Korea
Dockyu Kim
Search for Dhiraj Kumar Chaudhary in:
Search for Dong-Uk Kim in:
Search for Dockyu Kim in:
Search for Jaisoo Kim in:
D.K.C. and D.-U.K. conceived, designed and conducted all the experiments. D.K. involved in sample collection and data interpretation. J.K. coordinated and supervised the study. D.K.C. and D.-U.K. analyzed all the data and wrote the manuscript. All authors read, discussed, edited and approved the final draft of the manuscript.
Correspondence to Jaisoo Kim.
The authors declare no competing interests.
Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional Tables and Figures
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Chaudhary, D.K., Kim, D., Kim, D. et al. Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil. Sci Rep 9, 4134 (2019) doi:10.1038/s41598-019-40667-7
Flavobacterium silvisoli sp. nov., isolated from forest soil
, Ram Hari Dahal
International Journal of Systematic and Evolutionary Microbiology (2019)
Flavobacterium cellulosilyticum sp. nov., a novel psychrophilic bacterium isolated from Arctic soil
, Altankhuu Khulan
, Dong-Uk Kim
Scientific Reports menu
About Scientific Reports
Guest Edited Collections
Scientific Reports Top 100 2017
Scientific Reports Top 10 2018
Editorial Board Highlights


E-learning is one of the most talked about topics in many learning institutions. And it's for a good reason.
According to The American Academy, when implemented correctly, this new alternative mode of learning can produce great results by decreasing the cost of learning while also providing accurate scalability of the performance matrix.
Unlike the normal classroom setup, an eLearning course is available for a broader range of students. This also involves static eLearning courses as well as the active communication of online boards. Below you will find the different unique value points of eLearning to the whole constitution and landscape of education.
Same as the learning content, the students don't have to bring heavy reference materials and books to class anymore. Everything they'll ever need in a virtual class will be available in the learning portal.
There are fewer tardiness or absences in online learning because the students can log in and study right away. It's a great solution for students who have day jobs and can only study at night.
Every student can expect that all topics will be presented in a uniform manner. Since eLearning comes from software, content delivery is always consistent across all sessions.
Compared to the traditional learning setup, failing is a stigma that everyone avoids. But, with eLearning when students fail, this simply means that they have to try again. Something you can't always do on a regular class.
With the shift in learning preferences among students today, and the time restraint present in the modern setup, online education is the alternative everyone needs.


text:
Q: How to play a sound with AVAudioPlayer every Monday using Swift? How can I play a sound with AVAudioPlayer every Monday using Swift?
I have an AVAudioPlayer setup and ready to play a sound at a specific time.
My issue is that when I select which weekday (2 = monday) that I would like to have my sound play and repeat every time the current weekday matches the selected weekday and convert this to DateComponents I need to get the day, month, and year from the selectedDateTime when these field are not needed because I only care about having this sound repeat every week at the selected weekday.  If I do not provide the day, month, year the sound will not play and the timeInterval become negative.
var dateComponents = DateComponents()
                dateComponents.hour = calendar.component(.hour, from: selectedDateTime)
                dateComponents.minute = calendar.component(.minute, from: selectedDateTime)
                dateComponents.timeZone = .current
                dateComponents.weekday = 2

// should not be needed (below)
                dateComponents.day = calendar.component(.day, from: selectedDateTime)
                dateComponents.month = calendar.component(.month, from: selectedDateTime)
                dateComponents.year = calendar.component(.year, from: selectedDateTime)

What will allow me to schedule and play an AVAudioPlayer every monday?
Please note that I am intending the user to keep the app open in the background.


Guy Lagendyk - Essentia, Inc.
Guy brings to Essentia a wealth of telecommunications technical and managerial experience. With 22 years of wireless technical knowledge Guy is providing the guidance and support to help Essentia and our customers to navigate through the exciting fast changing technological advances of wireless 4G and 5G networking deployment.
Guy lagendyk has spent over 37 years in the telecommunications engineering profession holding a multitude of engineering and operations leadership roles. The last 22 years Guy held several executive level engineering and operations positions with Verizon Wireless including Director Of Operations, Director Of Engineering, and Director of System Performance. In those roles Guy led a highly experienced team of professionals in the design, planning, and implementation of Verizon's industry leading and award winning 4G and 5G based data and voice wireless network.
Guys defining achievement was overseeing the planning, design , budgetary management, and deployment of Verizon's evolution from CDMA wireless technology to today's state of the art 4G LTE and 5G network over a four state market in the Midwest. This achievement encompassed hundreds of millions of dollars of 4G LTE network equipment including the placement of macro and small cells, LTE and VoLTE network elements, CRAN/LTE Advanced technologies, switching equipment, and in-building and large venue DAS systems.


Donald Trump. U.S. President Donald Trump.
South African business leaders plan to boycott United States President Donald Trump's address to delegates at the World Economic Forum's meeting in Davos next week' because of his divisive "shithole" comments about Africa' El Salvador and Haiti and failure to apologise for it.
Bonang Mohale' CEO for Business Leadership South Africa' in an open letter to Trump' encouraging likeminded peers to join the boycott.
"It is our hope' however' that your presence will help stimulate a debate that inspires commitment to a world premised on basic principles of humanity' inclusivity' respect' tolerance and forbearance. "An alternative' in other words' to a world where walls' disparagement and hate dominate the discourse of the leader of the US'" Mohale wrote.
He said the overt racism of Trump's statements were self-evident and that the American president had "cast a pall" over the symbiotic relationship between Africa and the US "by impugning the dignity of 1.2 billion Africans".
"One can only imagine how these views have been received by the millions of Americans descended from the slaves' who were herded and sold like cattle' and shipped to the US to build its economy. In insulting the homeland of their ancestors – who had no say in the genocide and atrocity that was slavery – you have again violated the dignity of those with roots in Africa' wherever they are in the world'" Mohale added.
He said it was encouraging that many Americans' including people in Trump's Republican party' rejected the president's "monochrome vision" for the United States.
"We join hands with them' in the same spirit of solidarity that many of your citizens showed in rejecting Apartheid and isolating those who sought to entrench racism' segregation and discrimination'" Mohale wrote.


Andrea Luka Zimmerman is an artist, filmmaker and cultural activist. Andrea's work is concerned with marginalisation, social justice and structural violence and has been nominated for the Grierson and Jarman awards. Films include Erase and Forget (2017), which had its World Premiere at the Berlin Film Festival (nominated for the Original Documentary Award), Estate, a Reverie (2015) and Taskafa, Stories of the Street (2013), written and voiced by the late John Berger. Selected exhibitions include Civil Rites, the London Open, Whitechapel Gallery and Common Ground, Spike Island, Bristol. She co-founded the cultural collectives Fugitive Images and Vision Machine (collaborators on Academy Award® nominated feature documentary The Look of Silence).


text:
Гавриил Державин () роден на 3 [14] юли 1743 в село Сокура, Казанска губерния, починал на 8 [20] юли 1816 в имението Званка, Новгородска губерния е руски поет от епохата на Просвещението, известен държавник на Руската империя, сенатор и действителен таен съветник.

Биография

Съгласно родова легенда родът му произхожда от татарите.

Гавриил се ражда в семейството на дребни дворяни край Казан и там прекарва детството си. От 1762 г. служи като редови гвардеец в Преображенския полк, като в състава на полка участва в държавния преврат на 28 юни 1762 г., в резултат на който на престола застава Екатерина II. През 1773—1775 години участва в потушаването на въстанието на Емелян Пугачов. Записките на Державин представляват ценен исторически източник, тъй като той има непосредствена информация за него. Пушкин използва тези записки при създаването "Историята на бунта на Пугачов" и Капитанската дъщеря.

Творчество

Първите му стихове са публикувани през 1773 г. Произведенията ме отразяват блясъка и разложението на епохата на Екатерина II:
 Днес всеки, мъдър или знатен,
 живее в някаква лъжа!
 Мечтите за разврат сменили,
 не търсим вече светли сили.

При неговата поезия елементите на сатирата се съчетават с елементи на интимно-лирическата поезия. Г. Державин е новатор в областта на художествената форма и творчеството му е преход от класицизма към реализма. Неговото творчество влияе на развитието на поезията на Пушкин.

Пушкин отразява свота среща с Державин в Евгени Онегин:
 С усмивка срещна я светът,
 успехът е да окрилява;
 Державин, от задгробен кът,
 с любезен жест ни благословява.

Източници

Външни препратки

Руски поети
Руски драматурзи
Руски министри
Министри на правосъдието
Руски мемоаристи
Руски масони
Почетни граждани в Русия
Татари в Русия


Best love match for a virgo woman.
If there is something Scorpio would like to fight for, it is the chastity of Virgo.
Typically, this is an odd match, considering that it involves the bold and arrogant Leo man and the modest and quiet Virgo woman. Though this is a quite quirky relationship, it can certainly work if both partners observe a strong mutual respect.
If you treat a Virgo well, they will treat you even better … One night stands are usually not their style.
Here through Google or some other search engine? I hope you find this post informative, I would like to add that this post is made up of content taken from different astrology sites only because I am a Virgo Woman.
Virgos share strong immunity and have a good healthy life.
When Virgo woman falls in love, she will probably get scared at first. If this happens outside of a relationship, she will probably be so shy that it will become impossible for her to start a relationship at all.
When Virgo and Leo join together in a love match, they may initially overlook common interests and feel they have nothing to gain from one another. This is a relationship that evolves over time, each partner gradually understanding and appreciating the other. Leo is extroverted, dominant, and.
You have good taste when it comes to either making or consuming a meal.
Compatibility Virgo: This section highlights Virgo compatibility in combination with each of the sun signs in the zodiac. Each love match has different strengths and .
Each is inclined to say what they mean and mean what they say.
Virgo is born between August 23 – September 22 Virgo is the Fifth Sign of Zodiac and is traditionally represented by a virgin girl. Read on to find out about the Virgoan personality in detail, their strengths and weaknesses, what they are like in love and relationships, and the best way to attract a Virgo partner.
They are quick to make decisions when something is no longer matchmaking beta, and they can easily find ways to cope and adapt to major changes in their lives.
Lack of Shared Interests. The Aries man seeks adventure in his love life, while the Virgo woman seeks romance and www.tgcbtr.org Aries man changes for nobody, while the Virgo woman seeks to shape and mould her man into a better version of himself.


text:
Reading: Top incidents, crimes, action taken against criminals and weather report of the day
Uitvconnect : Breaking News, India News, Sports News and Live Updates > Blog > Indian > Top incidents, crimes, action taken against criminals and weather report of the day
Top incidents, crimes, action taken against criminals and weather report of the day
Get all the latest incidents, crimes, diseases, and weather reports of today.
Man commits suicide amid marital strife
A man going through marital strife allegedly hanged himself from a ceiling fan of his house in North Delhi's Lahori Gate area, said an official on Tuesday.
The deceased has been identified as Ashif Mumtaj (40), a resident of Farashkhana near Lahori Gate area.
According to Sagar Singh Kalsi, the Deputy Commissioner of Police (north), on Monday an information was received regarding a man hanging from a fan following which a police team reached the spot.
"On reaching the spot, the police found a man hanging from a ceiling fan with the help of a white cloth. The caller was Wasim, the brother of Ashif who was passing through a marital discord," said the official.
"Ashif had got married about eight years ago and had two children. He runs a footwear shop at Wazirabad. On Monday, a family meeting was held which decided that the couple would live separately," said the official.
"A crime team is carrying on an inspection while legal proceedings under section 174 code of criminal procedure (CrPC) are underway," he said.
Kodanad heist & murder case: Police question kin of dead computer operator
In a new turn to the infamous Kodanad estate murder and heist case, the CB- CID team of Tamil Nadu Police visited the residence of Dhinesh Kumar, computer operator of the estate office, who committed suicide on July 3, 2017.
A seven-member team of CB-CID officers questioned Dhinesh Kumar's parents and other relatives and friends. The police team visited the residence of Dinesh Kumar where he was found dead under suspicious circumstances.
The DMK had in its election manifesto had stated that it would conduct a reinvestigation into the Kodanad murder cum heist case if it was voted back to power.
Kodanad estate is jointly owned by former Chief Minister of Tamil Nadu, Late J. Jayalalithaa and her close aide and former interim general secretary of AIADMK, V.K. Sasikala.
After the death of Jayalalitha on December 5, 2016 and the arrest of Sasikala in February, 2017 in a disproportionate asset case, there was a major heist in Kodanad estate on April 23 and 24, 2017 in which the guard of the estate Om Bahadur died.
Another watchman, Krishna Thapa was found grievously injured in an attack and he told the police that a group of 10 men had arrived at the estate and attacked them.
The first accused in the case was Kanakarajan, former driver of Jayalalithaa. In a twist of events, Kanakarajan died in a road accident when he was hit by a speeding truck on the Salem- Chennai highway five days after the Kodanad heist. On the same day, the second accused, Sayan, and his family met with an accident at Walayar in Palakkad district in which his wife and daughter died and Sayan escaped.
Dinesh Kumar, the computer operator of the Kodanad estate was found hanging in his house under mysterious circumstances on July 3, 2017.
The DMK government has constituted a special team of CB-CID sleuths of Tamil Nadu Police to conduct a re-investigation into the case.
Dense fog envelopes Delhi, reduces visibility
Dense fog enveloped Delhi and parts of northern India on Tuesday, lowering visibility to 25 metres in the national capital and affecting traffic movement.
"The lowest visibility reported at 5:30 hours IST Dated 20.12.2022: Bhatinda: 00; Amritsar: 25; Ganganagar: 25; Patiala: 25; Delhi (Palam): 25; Lucknow: 25; Delhi (SFD): 50; Purnea: 50; Ambala: 200; Agra: 200; Gorakhpur: 300; Bareilly: 500; Patna: 500; Gaya: 500; Kolkata: 500," the India Meteorological Department (IMD) tweeted.
The IMD has also issued a 'cold wave' alert in many other states, including Uttar Pradesh and Punjab.
As per the Department, the minimum temperature in Delhi on Tuesday was 6 degrees Celsius, and the maximum temperature is expected to be 22 degrees.
"Following the light winds at lower tropospheric levels over the Indo-Gangetic plains and moisture, dense to very dense fog in is very likely to be in some pockets over Punjab, Haryana, Chandigarh, Delhi, Himachal Pradesh, and Uttar Pradesh during the next three days in the night and morning hours and dense fog in isolated pockets for subsequent two days," an IMD official said on Tuesday.
The Department also said that some parts of Bihar, West Bengal, and Sikkim may also experience dense fog during the next two days.
Minimum temperatures are in the range of 2-4 degrees Celsius over Punjab, Haryana and north Rajasthan.
"Due to dry north/northwesterly winds from the Himalayas, cold wave conditions are likely to continue in isolated pockets over Himachal Pradesh, Punjab, Haryana, and north Rajasthan during the next 4-5 days," it said.
The IMD said that there will be no significant change in minimum temperature over the next 4-5 days.
Couple in UP booked for forced conversion to Christianity
A couple, visiting Sitapur along with tourists from Brazil, has been booked under the UP Prohibition of Unlawful Conversion of Religion Act for allegedly forcing people to convert to Christianity.
The main accused, David Ashthana, was arrested late on Monday night.
Naimish Gupta, a resident of Sehbajpur village, lodged an FIR alleging that a couple from Lucknow, David Asthana and Rohini Asthana, reached their village with Brazilian tourists, identified as Rivaldo Joses DaSilva, Magnolia Maro Laronzera, Gulheram Nasimento Edalgo and Alexander D'Silva.
"They met the villagers and tried to force them to convert," Gupta said.
Circle Officer Mahmoodabad, Ravi Shankar Prasad, said, "A police team reached the village after getting information from Naimish Gupta. The Brazilians informed us that they were here on a tourist visa and were visiting Sitapur with the couple from Lucknow. Investigation is underway and the tourists will be questioned. We have registered a case against David and his wife. They were not able to explain why they took the tourists to a small village in Sitapur," Prasad said.
He said that the statements of villagers would also be recorded before initiating further action.
Police said it is yet to be established how the tourists came in touch with the couple.
Girl dies of suffocation from gas geyser in UP
A 17-year-old girl died due to suffocation in the washroom in Kandla town of the district.
Ishika Goel, a Class 12 student, had gone to take a bath, but when she did not come out of the washroom for long, the family broke open the door and found her lying unconscious.
She was rushed to the hospital where doctors declared her dead.
The cause of death is stated to be the release of gas from a geyser and non-ventilation.
The deceased's father Sanjay Goyal, who is a trader, said, "The family members are in shock."
Shamli health department medical officer, Dr Anupam Saxena said, "Gas geysers are usually present in homes, but proper arrangements must be made for their ventilation."
3 LeT terrorists killed in Kashmir's Shopian
Three Lashkar-e-Taiba (LeT) terrorists were killed in an encounter with security forces in South Kashmir's Shopian district, officials said on Tuesday.
"Among the three neutralised local terrorists, two identified as Lateef Lone of Shopian, involved in killing of a Kashmiri Pandit Purana Krishna Bhat; and Umer Nazir of Anantnag, involved in killing of Till Bahadur Thapa of Nepal. One AK-47 rifle and two pistols recovered," the J&K Police quoting Additional Director General Police Kashmir zone Vijay Kumar said in a tweet.
The encounter started after a joint team of police and security forces received a tip-off about the presence of terrorists in that the Munjh Marg area.
After the area was cordoned off, terrorists started firing drawing retaliation by the security forces.
UP: BJP MP acquitted in 29-yr-old murder case
An MP-MLA court in Uttar Pradesh's Gonda district has absolved BJP MP from Kaiserganj , Brij Bhushan Sharan Singh, and two others of all charges in a 29-year-old case related with the attack on former Samajwadi Party Cabinet Minister Vinod Kumar Singh a.k.a. Pandit Singh in 1993.
Pandit Singh died due to Covid-19 on May 7, 2021.
Jitendra Gupta, Additional District Judge, MP/MLA court, acquitted Brij Bhushan Sharan Singh, Gyan Singh and Deep Narain Yadav Pehlwan of all charges due to lack of evidence.
Pandit Singh's relative Yogendra Singh had lodged an FIR on December 24, 1993, at Nawabganj police station, Gonda, alleging that four car-borne miscreants attacked Pandit Singh when he was standing outside his house.
After firing several rounds, the miscreants fled presuming that Pandit Singh was dead.
Lawyers Srikant Srivastava, Bhupeshwar Upadhyay and Upendra Mishra, who represented the BJP MP in the court, said the prosecution was not able to prove the charges in the court.
At the time of attack, Brij Bhushan Sharan Singh was at his Delhi residence, the lawyers added.
5 held in Assam for performing bike stunts on national highway
A total of five persons were held in Assam's Hailakandi district for performing risky bike stunts on the national highway, the police said on Monday.
A senior police officer said that five youths were doing bike stunts on the national highway that connects Hailakandi with another nearby district Karimganj.
Police arrested all of them, identified as Amin Laskar, Abdur Rahman Laskar, Fakrul Islam Laskar, Abdul Aziz, and Iqbal Hussain Majumdar.
The bike stunts of the group caught the attention of the police after one of them uploaded a video on social media.
Earlier, special DGP of Assam G.P. Singh had warned the bikers who try to perform dangerous stunts on the highway.
Singh wrote on Twitter: "People of Assam are requested to inform through Direct Message about any place or persons indulging in stunt biking on a regular basis. Such displays on social media platforms may also be brought to the notice of Assam Police."
On Monday, Chief Minister Himanta Biswa Sarma also directed all district administration officials to ensure proper safety measures to bring down road accidents in Assam.
NIA arrests 9 Sri Lankans from TN camp in arms, drug supply case
The National Investigation Agency (NIA) on Monday arrested nine Sri Lankans from a special camp in Tamil Nadu's Trichy in a case relating to illegal drugs and arms trade in Sri Lanka and India.
The accused were identified as C. Gunashekharan alias Guna alias Prem Kumar, Pushparajah alias Pookutti Kanna, Mohammad Asmin, Alahapperumaga Sunil Ghamini Fonseca, Stanly Kennady Fernando, Ladiya, Dhanukka Roshan, Vella Suranka alias Gamage Suranga Pradeep, and Thilipan alias Dileepan.
The case pertains to activities of a Sri Lankan drug mafia controlled by Gunashekharan and Pushparajah, in association with Pakistan-based arms and drug supplier Haji Salim, operating in illegal drugs and arms for the revival of the LTTE in India and Sri Lanka, and to further its violent activities.
The case was registered suo moto by the NIA on July 8 under various sections of the Indian Penal Code, the Unlawful Activities (Prevention) Act, and the Narcotic Drugs and Psychotropic Substances Act.
Further investigations in the matter are on.
Bengaluru man books maid service online, rapes and locks her up
A man in Bengaluru was arrested on Monday for allegedly raping a 21-year-old maid, whose services were booked online, and locking her up in his house, police said.
The accused was identified as private company employee Parashiva Murthy, 47, a resident of Kudlu under Parappana Agrahara police station in the outskirts of the city.
According to police, the accused booked the maid on the 'Book My Bai' app. He maintained that he needed maid service for home chores and to look after his aged parents.
Based on the request, the 21-year-old woman was sent to his home from the Wilson Garden office of the 'Book My Bai' app. Murthyhad raped her when she came to work and threatened to kill her that if she told anyone about this to anyone, he would kill her.
He further locked her up in his house and went out to work. The girl had informed about her situation over the phone to the office. The office staff have complained the matter to the Parappana Agrahara police station.
The police broke open the door of the house and rescued the victim. Later, they arrested the accused and took up further investigation.
Rupert Grint says filming 'Harry Potter' was 'suffocating'
After layoffs, Zuckerberg now wants 2023 to be 'year of efficiency'
TAGGED: featured, Home Slider


This entry was posted in ET Contact Reports 2013, Photos of Interest.
I'm extremely interested in your works. I've had my own personal experiences that I cannot explain. If I could share my view with someone who would understand if appreciate it.
I'm an Alberta resident, Calgary to be specific. I've come across startling experiences that have made me wonder, wonder why these strange phenomenon I call keep happening to me. I can't explain but I know now that I'm not alone. We're not alone.


I have quite a while to observe in Eden on Arbat Goglovoy Lyudmila Yurevna and I want to say that it is a very professional approach to business. I have only positive impressions remain after a visit to her. Yes, it can, and get upset when something is found from the sores, but without them nothing.
Infectious diseases hospital № 2 Zaporozhye children's department - the terrible conditions and the incompetence of the staff!
Kirovograd Regional Hospital - make money on the recruits.
With the passage of conscription medical examination of my son sent for further examination at the regional hospital.


Courtyard by Marriott Chicago Downtown Magnificent Mile is offering rewarding new packages for Chicago wedding planners. The hotel is offering a special discount on reception dining and offers that provide a full suite of wedding-weekend amenities and services.
The Free Weddings offer provides a discount on the delicious catering provided by Viand at Courtyard Chicago.
Courtyard by Marriott Chicago Downtown Magnificent Mile is excited to announce new specials meant to make weddings in the heart of Chicago easy, affordable, and memorable.
The Free Weddings offer provides a discount on the delicious catering provided by Viand at Courtyard Chicago. For each weekend room night booked as part of the wedding room block at Courtyard, receive a free dinner entrée at your reception. When the wedding party and guests make plans to stay at Courtyard Magnificent Mile, enjoy savings.
Courtyard Chicago Magnificent Mile provides a wide range of wedding services and amenities. The hotel's new Silver and Gold Wedding Packages ensure every guest will enjoy fine dining and elegant décor, while the bride and groom will receive premier perks. Starting at $94 per guest, the Silver and Gold packages provide an array of add-ons, ranging from elegant linens and china to champagne and chocolates for the bride and groom and a complimentary bridal suite.
Located just steps from the iconic skyscrapers and the stellar shopping of Michigan Avenue, Courtyard Chicago Magnificent Mile serves as a quiet and intimate venue in the heart of the Chicago Loop. The hotel, which offers stunning views of the city skyline and Lake Michigan, provides customized menu planning and an array of personal touches in the Ontario Ballroom, an elegant room with space for up to 200 guests.
Courtyard Chicago Magnificent Mile provides comfortable accommodations and elegant event spaces in the heart of Downtown Chicago. The hotel features 306 oversize guest rooms and suites, outfitted with luxurious bedding and ample amenities to make guests feel at home. Onsite features include contemporary American dining and hand-crafted drinks at Viand Bar and Kitchen, convenient coffee at Starbucks, 4,000 square feet of flexible meeting and function space, and an experienced team of expert event planners. Located just steps from Michigan Avenue and within easy reach of all the best sights, sounds and shopping Chicago has to offer, Courtyard Chicago is ideal for leisure travel, corporate visits or stellar Chicago events.