I am new to algae expression and would like to know the similarities and differences between using Synechococcus elongatus and Chlamydomonas reinhardtii. Do you have a comparison table?
Can be a problem, dependent on the gene sequence and protein toxicity; pChlamy_4 vector is designed so that proteins are expressed as transcriptional fusion with sh-ble, providing higher expression levels. The Zeocin selection helps to maintain gene expression during passage.
* The original pSyn_1 vector contained the PNI promoter, which was induced by nickel; however, when too much nickel was added, it became toxic to the cells, and therefore, the promoter was changed to Psc. Nickel induction is no longer recommended.
**The original version, pChlamy_1 vector, does not have a 3' UTR and should be added to your insert immediately following the stop codon for expressing high levels of recombinant protein.
Do you have data demonstrating protein expression levels after cloning into your algae vectors?
Graph A below depicts the enzyme xylanase cloned into vector pChlamy_4, and transformed into Chlamydomonas reinhardtii 137C. Activity was measured by EnzChek® Ultra Xylanase Assay Kit, and was 17.6-fold higher than that observed with previous systems. Graph B depicts the gene for B-glucuronidase (GUS) cloned into vector pSyn_6 and transformed into Synechococcus elongatus. GUS activity levels were >100-fold higher than those seen in our previous system.
What is the expected time constant during algae transformation?
The average expected time constant is 15–20 milliseconds. We have a Neon® electroporation protocol in the manual.
Is transfection transient or stable for algae expression?
It is a stable transfection.
Is there a potential for gene silencing when using your algae kits?
Yes, unfortunately, silencing is a big problem in algae. The extent of silencing depends on the sequence of the gene and the toxicity of the protein that expresses from that gene to the cell.
The engineering kit is designed for random insertion of your gene of interest, which can lead to your gene being lost quickly. The protein expression kit, which contains the pChlamy_4 vector, is designed so that proteins are expressed as transcriptional fusions with the bleomycin/zeocin resistance gene sh-ble (Rasala et al., 2012). The self-cleaving sequence for the 2A peptide from the foot-and-mouth disease virus (FMDV) is placed between the antibiotic resistance gene and the gene of interest. It encodes a short ~20 amino acid sequence that mediates proper cleavage to yield two discrete proteins. With this system we have seen positive transformants maintain high expression levels for much longer than with other systems, even after many passages with or without selection pressure.
Is the TAP media good for growing any other kind of green algae?
Unfortunately, we have not tested TAP media with other kinds of green algae. There is an indication that Chlorella will grow if the TAP media is vitamin-fortified, but we don't have the specifics.
What is the formulation of your TAP media?
What is the formulation of Blue-Green-11 (BG-11) media?
We offer ready-to-use BG-11 Media, optimized for Cyanobacteria (Cat. No. A1379901). There are several BG11 media formulations. Please see our formulation below.
Our BG-11 media have been qualified for culturing Synechococcus elongatus (strain PCC 7942).
Why are the algae kits branded as GeneArt® kits?
GeneArt® gene synthesis was used to create the plasmid backbones synthetically. Also, GeneArt® is the umbrella brand for synthetic biology, which is a large area in which these kits will be used.
How can I measure cell growth of algae cells?
I would like to create a stable algae line via non-random integration. Which kit would you recommend?
We recommend our Synechococcus kits, where the integration is directed to the Neutral Site 1 of the algae genome.
What is the shelf life/competency of the Chlamydomonas cells?
After 6 months of storage, the Chlamydomonas cells did not lose their competency. It is expected that they may with longer storage times, but we have yet to gather data points for loss of competency after one year of storage.
What is the strain of the Chlamydomonas cells you offer?
Does Chlamydomonas have a cell wall?
There are both cell wall minus and positive strains; 137c has a cell wall.
What should the cells look like upon arrival?
The Chlamydomonas cells should be dark green in color. Light green or even colorless (and maybe some droplets along the side of the vial) cells are indicative of freeze/thawing or fluctuations in temperature, which Chlamydomonas cells are extremely sensitive to.
How can I store my positively selected Chlamydomonas for long-term storage?
The kits are not designed for long-term storage of positive-selected clones. Most people keep the plates on their bench top at room temperature (not 4°C, as they need light) while in use, re-streaking if necessary. The GeneArt® Cryopreservation Kit for Algae can be used to preserve algal strains and clones for storage at –80°C for years.
Can green algae other than Chlamydomonas be transformed with pChlamy vectors?
Unfortunately, we have not yet tested other algae.
Is transfection with your pChlamy vectors transient or stable?
Transfection with these vectors is a stable transfection.
What is the mechanism of integration with the Chlamydomonas engineering kits?
Integration is at random. The engineering kit is based on a random insertion in which the gene of interest can be lost very quickly. For the protein expression kit, the pChlamy_4 vector is designed so proteins are expressed as transcriptional fusions with the bleomycin/zeocin resistance gene sh-ble (Rasala et al., 2012). The self-cleaving sequence for the 2A peptide from the foot-and-mouth disease virus (FMDV) is placed between the antibiotic resistance gene and the gene of interest. It encodes a short ~20 amino acid sequence that mediates proper cleavage to yield two discrete proteins. With this system we have seen positive transformants maintain high expression levels for much longer than with other systems, even after many passages with or without selection pressure.
What is the expected transformation efficiency?
We don't report transformation efficiency of the Chlamydomonas cells, since the end result of transformation is random integration into the genome. The electroporation results will depend on the gene of interest. The control vector should produce a minimum of 30 transformants per electroporation reaction. The number of positive clones per colonies picked should be ≥90% with your gene of interest.
What is the expected time constant during transformation?
The expected time constant is 15–20 milliseconds (average is 17 milliseconds).
What is the shelf-life/competency of Chlamydomonas?
After 6 months of storage, the Chlamydomonas did not lose their competency. We have currently not tested beyond this time point.
I have an older version of the pChlamy vector, pChlamy_1. Do you recommend addition of a 3'UTR to the insert?
Yes, if you plan to use the pChlamy_1 vector to express high levels of recombinant protein, your insert also needs to contain a 3' UTR (untranslated region) immediately following the stop codon.
Do the pChlamy_3 vectors contain a termination sequence or a 3' UTR for your gene of interest?
Yes, the pChlamy_3 vector contains a 3' UTR after the multiple cloning site.
In the Chlamydomonas kit, there is already an ATG in the vector; does the insert need to be in frame with the ATG in the vector?
Yes. For the TOPO version, you can design the primer to make sure the coding sequence is in frame with the ATG in the vector.
Should I clone and insert not only the coding sequence but also the 3'UTR of my gene of interest?
The pChlamy_1 vector does not have the stop codon and the 3' UTR. The pChlamy_3 vector has the 3'UTR that has been shown to increase protein expression.
What is the difference between pChlamy_4 in the Chlamydomonas Protein Expression kit compared to pChlamy_1 or pChlamy_3 in the engineering kits?
The pChlamy_4 vector was designed in order to circumvent the transgene silencing that often occurs in C. reinhardtii. This vector is also designed so that proteins are expressed as transcriptional fusions with the blemoycin/zeocin resistance gene (sh-ble). The self-cleaving sequence for the 2A peptide from the foot-and-mouth-disease-virus (FMDV) is placed between the antibiotic resistance gene and the gene of interest. It encodes a short ~20 amino acid sequence that mediates proper cleavage to yield two discrete proteins. With this system we have seen positive transformants maintain high expression levels for much longer than with other systems, even after many passages with or without selection pressure.
Can I look at the vector maps to compare pChlamy_1, pChlamy_3, and pClamy_4?
What does the GeneArt Chlamydomonas Protein Expression Vector include?
It includes the pChlamy_4 vector. This vector has been tested for protein expression in Chlamydomonas reinhardtii 137c . You can get the Chlamydomonas reinhardtii 137c cells from the Chlamydomonas Resource Center (http://www.chlamycollection.org/).
What is the strain of Synechococcus used in your kits?
Synechococcus elongatus strain PCC 7942, a model cyanobacterium, is used.
What is the mechanism of integration when using the Synechococcus expression kits?
A double homologous recombination event occurs between the Neutral Site 1 in the genome and between NSa and NSb in the plasmid. This results in the gene of interest and spectinomycin resistance integrating into the genome within the Neutral Site 1.
What is the shelf-life/competency of Synechococcus?
After 6 months of storage, the Synechococcus did not lose their competency. We have not yet tested longer storage points.
What is the difference between pSyn_1 and pSyn_6?
The promoter for pSyn_1 is the Psc. Psc is a weak constitutive promoter that drives basal expression of your gene of interest. The promoter was taken from the solanesyl diphosphate synthase gene from Synechocystis sp. Strain PCC 6803. Note: Use of this weak constitutive promoter is a good fit for applications that are hindered by strong expression, such as pathway engineering or complementation of mutant genes normally expressed at low levels (Simkovsky et al., 2012).
The promoter for pSyn_6 is the psbA promoter (PpsbA). PpsbA is a strong constitutive promoter of the psbA gene (encoding photosystem II protein D1) from Synechococcus elongatus, driving high-level expression of your gene of interest. The pSyn-6 vector also includes a ribosome-binding site (GAAGGAG) for efficient initiation of translation, as well as a stop codon. A 6xHis TEV and a V5-His epitope tag is also included in the vector backbone for detection/purification of the gene of interest. NS1 (neutral site 1) homologous recombination sites are present in both vectors for the integration of the vector into the Synechococcus elongatus genome.
What does the GeneArt Synechococcus Protein Expression Vector include?
It includes the pSyn_6 vector. This vector has been tested for protein expression in the Synechococcus elongatus PPC 7942. You can get the Synechococcus elongatus PPC 7942 from academic resources (http://genome.jgi.doe.gov/synel/synel.home.html) or from the ATCC Collection (https://www.atcc.org/products/all/33912.aspx).
Do you offer a kit for the preservation of algal strains and clones?
Yes, we offer our GeneArt® Cryopreservation Kit for Algae (Cat. No. A24228).
How does the GeneArt® Cryopreservation Kit work?
Simply grow your cells in the presence of Cryopreservatin Reagent A for 2–5 days, harvest the cells, and resuspend them in Cryopreservation Reagent B. Follow by freezing the cells.
What is the cell viability when using the GeneArt® Cyropreservation Kit?
We have tested the kit with Chlamydomonas and Chlorella strains, showing a 5% and 100% cell viability, respectively.
What freezing container should I use along with the GeneArt® Cryopreservation Kit?
What type of algae can the GeneArt Cryopreservation Kit for Algae be used for?
The GeneArt Cryopreservation Kit for Algae was developed for preserving the Chlamydomonas reinhardtii 137c cells. We have reports from some customers who were successfully able to preserve other algae cells.
What reagent should I use for transformation of Chlamydomonas?
We offer our MAX Efficiency® Transformation Reagent for Algae (Cat. No. A24229). Eight different strains of Chlamydomonas reinhardtii, CC1690, CC2935, CC1009, CC4414, CC118, CC536, WT137c, and CC3395 wall(-), were transformed using MAX Efficiency ® Transformation Reagent for Algae and with TAP/sucrose reagent by electroporation. The comparison below shows that MAX Efficiency® Transformation Reagent for Algae resulted in >1000-fold colonies (right) over TAP/sucrose media in C. reinhardtii WT137c (below).
What type of algae can the MAX Efficiency Transformation Reagent for Algae be used for?
The MAX Efficiency Transformation Reagent for Algae was developed for transforming the pChlamy_4 vector into the Chlamydomonas reinhardtii 137c cells by electroporation.


On July 21, 2017, Atlus had registered the domain name "13SAR.JP," presumably for Vanillaware's upcoming title, 13 Sentinels: Aegis Rim.
That domain name has been updated as of September 6, 2017.
The update means that what was previously held for squatting purposes on Onamae nameservers as a landing page, has been moved to Atlus nameservers which the company uses for their official websites.
The website currently returns an HTTP 403 Forbidden status, so public access to the site has been prevented. The official domain being updated is an indication that it is being prepared to be used for the near future.
An updated teaser trailer for 13 Sentinels was shown by Atlus USA, alongside a localization announcement, at E3 2017.
With a possible incoming announcement, this month's TGS 2017 would be the reasonably expected venue to match the update.
There will be an SCEJA PlayStation pre-TGS conference on September 19th, and Atlus will be holding a live stream event on September 24th with its contents unannounced as of yet (following the Megaten Special Live Stream on September 23rd).
13 Sentinels: Aegis Rim was originally announced at the SCEJA Press Conference 2015.
In late July 2017, the "PQ2.JP" and "PERSONA-DANCE.JP" domains were updated in a similar fashion, hinting at an upcoming reveal. This was the case on August 2, 2017, when the "Persona Super Live P-Sound Bomb!!!!" concert took place, bringing about the announcements of the two Persona Dancing games and Persona Q2.
A similar situation occurred with the official Persona 5 domain name in 2013. It was registered by Index Corporation on June 25, 2013, and was hosted on Onamae nameservers, leading to their landing page. Shortly before the November 24, 2013 live stream where Persona 5 was announced, the domain was moved to Atlus nameservers.


The church is located at 236 Old Tappan Rd, Old Tappan, NJ. Presbyterian Church of the Palisades Non Profit's debt is $2.4M and they filed the Chapter 11 which is very rare for Korean church in NJ. The church borrowed $3M from Valley National Bank in 2004 with the collateral of the church building and adjacent vacant lot. The church was sued for foreclosure in Dec 2018.
The church is facing the sheriff sale on 5/10. An officer mentioned that the church can take care of the issue because the church has the asset of $8M.
NE Korean conference in PCUSA which is a mainline Protestant denomination in USA filed the complaint to the court to nullify the Chapter and also the conference voted to dissolve the church, but it was not successful.
The first creditor meeting will be held on 5/1/2019.
Ridgewood town in Bergen county, NJ is planning to improve public school facilities with $174M budget which was increased by $60M from $110.17M last year's budget. This budget plan will be finalized in 4/16 through some minor adjustment form public opinion. The school board is expected to receive the aid of $5.49M from the State which means property tax will be increased by 1.8% instead of 2%.
According to this budget planning, property tax will be increased by $174 based on the house value of $702,225.
The increased education budget will be used for changing part time teacher to full time status, increasing high school capacity, bathrooms improvement.
Some are criticizing the plan on improvement of bath rooms for $500,000.
According to "New Jersey family" recent report, Montgomery is #1 best town to live. The organization studied on 512 towns in NJ for life convenience, commuting hours, access to hospital, etc. Glenrock in Bergen county is #2. Northvale is #22 and Harrington Park is #28. Demarest is #31. Closter is #41. Tenafly is #64. Old Tappan is #72 and Cresskill is #87.
Leonia is #108. Fort Lee is #247 and Ridgefield is #288. Palisades Park is #382.
The Trump International Hotel and Towner at 1 Central Park in Manhattan, near the southwest corner of the Central Park is 44-story building with luxury condo and hotel suite which can go for $2,500 per night. The unit 32G, 2 Bedroom, 1,767 sf was purchased by a daughter of the republic of Condo president, long time target of anti-corruption investigation.
The transaction took place more than 2 years before Trump became president.
The buyer name on the record, Ecree used a Manhattan address at the law firm K&L Gates.
Unlike banks and other financial institutions, US real estate developers typically have not been subject to rules that require they check the backgrounds or sources of money of prospective buyers, including looking out for potential money laundering.
If it is reported to the Justice Department, it might be the case because it was purchased by seemingly embezzled money.
The Mayor will have a hearing for condemnation of the 3 acre lot between 35th St and 39th St in Manhattan. The city has a plan for making a park and prepared $374M to pay for the lot, which means $124M per acre – record high price for a park. Some real estate professionals and politicians are criticizing the mayor's plan because NYC financial is not so good.
Some report says NYC financials is so bad that NYC might file bankruptcy and Bill de Blasio administration is wasting the city budget.
The Hudson Yard development cost is about $25B and the entire project will be completed in 2025. Currently about a half of the project is done.
The Movado group who designs watches for the Movado group and other labels including Coach and Tommy Hilfiger rented 3,005 sf on the sixth floor of the 26-story building at 110 West 40th street, between Broadway and Ave of the Americas. The space will be used as show room and the lease is 2 year and 9 months.
According to Miller Samuel, Real Estate appraisal company, sales in Manhattan reduced by 2.7%, compared to the same 1st quarter in 2018, which means sales in Manhattan reduced continuously for 6 quarters. As for 1st quarter, it is the lowest 1st quarter since 2009.
According to Wall Street Journal, sales for commercial/condo during 1st quarter was reduced by 5.2%, compared to the 1st quarter last year. It is reduced by 18%, compared to 1st quarter in 2016 which was highest period.
According to the investment consulting company Mercer, 2 degrees temperature increase on the earth till 2030 will make investment in general very difficult, which leads to negative profits – financial sector, agricultural sector, industrial sector, consumer goods, etc.
Also it will impact Real Estate sector as well. If sea level arises due to temperature increase, properties' price on river front and ocean front will go down and people will move to inland more.
If 4 degrees increase till 2050, it's effect will be worse severely.
Tech/Other News : "Flying Car" will be on the market in Netherland in Summer in 2020.


Create a fun fall themed pocket style page with the Thankful Double Sided Paper from the Fall Breeze Collection by Photo Play Paper. The package includes one 12" x 12" sheet of paper featuring fall cut outs on one side and a plaid pattern on the other. Designed by Traci Smith and made in the USA.


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Yes and the additional R2 policies in GPO to block everything except if your an admin or power user. That resource kit tool doesn't get rid of it, already tried. Please try again shortly.
Familiar printing experience— the HP UPD provides a consistent interface across a variety of HP printers, reducing the need for training and support.
In case hp universal pcl 5 intend to apply this driver, you have to make sure that the present package is suitable for your unicersal model and manufacturer, and then check to see if the version is compatible with your computer operating system. After installation, restart the computer.
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In the single file download, the PARK contains hp universal pcl 5 supported and unsupported software tools, scripts and hp universal pcl 5.
hp universal pcl 5 To block the driver installation via Point and Print yous must configure the following Computer policy See screenshot in atatched file. Do not forget to check with our site as often as possible in order to stay updated on the latest drivers, software and games. After downloading and installing the. Make hp universal pcl 5 you delete this key, the driver update will not clean it.
I have tried this obvious method before with same results. It is highly recommended to always use the most recent driver version available.
HP is aware of the recent vulnerabilities commonly referred to as "Spectre" and "Meltdown". HP Business Inkjet Legend.
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First Reconciliation (Confession) and First Communion (Holy Eucharist) preparation can begin at the age of reason, which is seven years old. As a rule, we encourage families to prepare for these sacraments during or after second grade by enrolling both in LIFT classes once a month for the entire family and in sacrament classes for the student and one or both parents. First Reconciliation and First Communion classes are usually one Sunday a month in addition to LIFT classes, plus a couple of extra events and practices during the year.


A deeply thoughtful and analytical chef, Jeremy Chan takes the scientific principles of flavour and applies them to create undeniably delicious food at London's Michelin-starred Ikoyi.
Amid the twists and turns of Jeremy Chan's striking, evocative tasting menus at Ikoyi, what really hits home is the attention to detail. Whilst London is embracing a restaurant trend of rustic, relaxed and home-style cooking, Jeremy's dishes emerge from the kitchen like art exhibits – intricately designed and impeccably crafted.
The 'experience' he refers to – the pleasure that comes with good food and good company – is the foundation of his cooking, and it always has been. Jeremy's parents instilled the importance of food and family from an early age, and Jeremy started cooking as a way to ensure his family could spend as much time as possible together on the rare occasions they were all united. 'We didn't have the whole family together very often,' he explains, 'so when we did, it was always for a meal. I started cooking at about fifteen years old as a way to speed up and improve the meals, so we could make the most of our time together.' Jeremy's obsessive streak was present even then – he would spend days researching and testing meals for his family ahead of their reunions.
Jeremy has always has a prodigious, borderline eidetic capacity for learning. As a teenager, he taught himself to speak six languages fluently because he found them interesting, and later added Farsi to the collection when he studied philosophy and theory of languages at Princeton University. 'When I'm interested in something, I want to know everything about it,' he explains. 'I was interested in languages in my teenage years, but that flipped onto cooking later on.' Jeremy fuelled his hunger for knowledge with cookbooks, reading hundreds of them – he would absorb them page by page, then move onto the next one.
Jeremy regularly organises collaboration dinners with chefs from all over the world, including Hiroyasu Kawate from Florilège in Tokyo, Ben Chapman from Kiln and Quo Vadis chef Jeremy Lee.
He still speaks French, Spanish, English, Portugese, Italian and Chinese fluently, but admits that his Farsi is a bit rusty.
Jeremy was born in the UK, but grew up between Hong Kong, Canada and the USA, picking up a hugely diverse range of culinary influences along the way.
Undoubtedly one of London's most exciting restaurants, Ikoyi's dynamic, West African-inspired menu creates food for thought, offering intelligent, precise and unique cooking in the heart of the city.


text:
Trump dismisses large demonstration that greeted him outside Pittsburgh synagogue as 'small protest'
Trump, the first lady, Ivanka Trump and Jared Kushner went to Pittsburgh.
ByEmily Shapiro, Jordyn Phelps, and Brian Hartman
People protesting against President Donald Trump wait near the Tree of Life Congregation on Oct. 30, 2018 in Pittsburgh.
Brendan Smialowski/AFP/Getty Images
When President Donald Trump visited Pittsburgh's Tree of Life synagogue Tuesday in the wake of a shooting massacre that killed 11, he was greeted by thousands of protesters nearby.
The president dismissed the demonstration Wednesday morning, tweeting that the "small protest was not seen by us, staged far away."
"The Office of the President was shown great respect on a very sad & solemn day," Trump tweeted. "We were treated so warmly."
The president visited Tuesday with first lady Melania Trump, his daughter Ivanka Trump and son-in-law Jared Kushner in the aftermath of Saturday's bloodbath by a gunman who allegedly spewed anti-Semitic statements as he gunned down worshippers.
Trump and his family were greeted by the synagogue's rabbi, Jeffrey Myers, who escaped the bullets. But outside nearby were thousands of people from different faiths, apparently united in anger, who marched toward the temple as the president and his family arrived.
Rev. Susan Rothenberg, a Presbyterian minister who lives down the street from the synagogue, was among those shouting and holding banners -- and she encouraged other neighbors, including children, to join her.
"Words have meaning!" some neighbors screamed toward the president.
(MORE: 'Words from our leaders matter': Biden scolds Trump's synagogue shooting response)
"I'm just going to pay my respects," Trump told Fox News on Monday prior to the visit. "I'm also going to the hospital to see the officers and some of the people that were so badly hurt. So -- and I really look forward to going -- I would have done it even sooner, but I didn't want to disrupt anymore."
Rabbi Myers and others greeted Trump outside the synagogue, where the president, the first lady, Ivanka Trump and Kushner lit a candle with the rabbis and Israeli Ambassador Ron Dermer in honor of the 11 victims.
White House senior advisers Jared Kushner and Ivanka Trump speak with Rabbi Jeffrey Myers as President Donald Trump and first lady Melania Trump pay their respects outside the Tree of Life synagogue in the wake of the shooting at the synagogue where 11 people were killed and six people were wounded in Pittsburgh, Pennsylvania, Oct. 30, 2018.
The president also placed stones, as part of Jewish tradition, and white roses on the Stars of David set up in a makeshift memorial for those killed.
President Donald Trump and first lady Melania Trump put down stones from the White House at a memorial outside for those killed at the Pittsburgh's Tree of Life Synagogue in Pittsburgh, Oct. 30, 2018, as Tree of Life Rabbi Jeffrey Myers watches.
After visiting the synagogue, the president and first lady went to a hospital where they met with medical staff and officers who were wounded in the attack.
Meanwhile, a community is left grieving.
Three funerals are to be held Wednesday, following the first two on Tuesday.
Dr. Jerry Rabinowitz and brothers Cecil and David Rosenthal were among those gunned down by a shooter inside the Tree of Life synagogue. Rabinowitz's friends and family gathered at his funeral Tuesday morning, while the Rosenthal brothers were honored at a joint service Tuesday afternoon.
A mourner reacts outside Rodef Shalom Congregation before the funeral services for brothers Cecil and David Rosenthal, Oct. 30, 2018, in Pittsburgh.
Mourners gather outside Rodef Shalom Congregation before the funeral services for brothers Cecil and David Rosenthal, Oct. 30, 2018, in Pittsburgh.
(MORE: Why Jewish hospital president checked on injured synagogue shooting suspect)
Rabinowitz, 66, was killed when he ran toward gunfire in the synagogue to try to help victims, according to his nephew, Avishai Ostrin.
"In addition to being the president of the congregation, he was a doctor, a healer ... when he heard shots he ran outside to try and see if anyone was hurt and needed a doctor. That was Uncle Jerry, that's just what he did," Ostrin wrote on Facebook.
Jerry Rabinowitz is seen here in this undated file photo.
Courtesy Rabinowitz Family
"Jerry was above all one of the kindest physicians and human beings in our community," University of Pittsburgh Medical Center officials said in a statement. The hospital network said it "cannot even begin to express the sadness and grief we feel over the loss of Dr. Jerry Rabinowitz."
(MORE: Portraits of the 11 synagogue shooting victims)
Slain brothers Cecil Rosenthal, 59, and David Rosenthal, 54, never missed a service and were always at the synagogue because it was a place they felt the most safe, fellow congregant Scott Levin told ABC News.
The two brothers were developmentally disabled.
"Cecil and David had a love for life and for those around them," according to a statement from ACHIEVA, a local organization which provides support for people with disabilities.
David and Cecil Rosenthal are seen here in this undated file photo.
(MORE: Pittsburgh synagogue rabbi says massacre that killed 11 not just against Jews but 'an attack on America')
"Cecil's laugh was infectious. David was so kind and had such a gentle spirit," Chris Schopf, Vice President of ACHIEVA Residential Supports, said in the statement. "Together, they looked out for one another. They were inseparable. Most of all, they were kind, good people with a strong faith and respect for everyone."
A mother and child place flowers, Oct. 29, 2018, at a memorial for victims of the mass shooting at the Tree Of Life Synagogue, in Pittsburgh.
(MORE: Pittsburgh synagogue shooting prompts wide outpouring of public support)
"All of Pennsylvania stands with the victims and their families," Pennsylvania Gov. Tom Wolf tweeted Monday as the state capitol building was lit in tribute. "We join the Pittsburgh Jewish community and Jewish Americans across our nation in mourning."
(MORE: Top leaders in Congress won't appear with Trump in Pittsburgh)
Trump on Saturday strongly condemned the shooting in Pittsburgh as "evil."
People stand in front of flowers and candles placed below a police cordon outside the Tree of Life Synagogue after a fatal shooting in Pittsburgh, Oct. 27, 2018.
Brendan Smialowski /AFP/Getty Images
Early on Saturday, just a few hours after the shooting, the president said, "It's presumed that this is a case where if they had an armed guard inside, they might have been able to stop him immediately ... Maybe nobody would have been killed except for him. So it's a very, very difficult situation."
When a reporter asked if all synagogues and churches should have armed guards, Trump said, "I hate to think of it that way ... It's certainly an option."
Charges against suspected gunman Robert Bowers include 11 counts of obstruction of exercise of religious beliefs resulting in death and 11 counts of use of a firearm to commit murder.
Robert Bowers is pictured in a booking photo released by the Butler County Prison on Oct. 30, 2018.
Butler Co. Prison
Bowers, 46, faces 22 charges that make him eligible for the death penalty, prosecutors said.
He is being held in the Butler County Prison.
Pittsburgh Synagogue Shooting

redpajama_set_name: RedPajamaCommonCrawl


text:
Q: Tabulartor error: "Sort Warning - Sort field does not exist and is being ignored tabulator" I have a table which looks like this (I'm using flask and jinja2):
     dataTable = {{ json_data | tojson }};
     var   view_doc = new Tabulator(
        "#view_doc",
         {
          layout:"fitDataFill",
          locale:"ru",
          data : dataTable,
          columns:{{ json_columnstr | tojson }},
          movableRows:true,
          //movableRowsConnectedTables: "#tablecolumns",
          dataTree:true,
          dataTreeChildField:"columns",
          movableRowsSender: "delete",
          //groupBy:"drilling",
         }

      );

and I'm trying to sort a column with dates:
view_doc.setSort({title:$("input[name=radiosort]:checked").val(), field:$("input[name=radiosort]:checked").attr("id").replace("radio", ""), sorter:"date", dir:active_sort, sorterParams:{format:"dd.MM.YYYY"}})

which evaluates to:

but it gives me this error: Sort Warning - Sort field does not exist and is being ignored.
The field and title are correct, so I'm not sure what is causing the error.

redpajama_set_name: RedPajamaStackExchange


Is it setting an actual B, C, P, or U code? If so what is the number?
Didn't have any codes a week ago. Just checked again after you posted. I have a code P0622 and no MIL. Wierd. At least I have something to work off of. Anybody see this code before?
agree point to point to point.
any clue would help now.
The engine control module (ECM) uses the generator field duty cycle signal circuit to monitor the duty cycle of the generator. The generator field duty cycle signal circuit connects to the high side of the field winding in the generator. A pulse width modulated (PWM) high side driver in the voltage regulator turns the field winding ON and OFF. The ECM uses the PWM signal input to determine the generator load on the engine. This allows the ECM to adjust the idle speed to compensate for high electrical loads.
The ECM monitors the state of the generator field duty cycle signal circuit. When the key is in the RUN position and the engine is OFF, the ECM should detect a duty cycle near 0 percent. However, when the engine is running, the duty cycle should be between 5 percent and 100 percent. The ECM monitors the PWM signal using a key ON test and a RUN test. During the tests, if the ECM detects an out of range PWM signal, DTC P0622 will set. When the DTC sets, the ECM will send a serial data message to the drivers information center (DIC) to display a warning message.
No generator, crankshaft position (CKP) sensors, or camshaft position (CMP) sensor DTCs are set.
No generator, CKP sensors, or CMP sensor DTCs are set.
The engine is less than 3,000 RPM .
During the key ON test, the ECM detects a PWM signal greater than 65 percent for at least 5 seconds .
During the RUN test, the ECM detects a PWM signal less than 5 percent for at least 15 seconds .
The ECM will not illuminate the malfunction indicator lamp (MIL).
The ECM will store the conditions present when the DTC set as Fail Records data only.
2xLS1, Thanks for all your help! I have amazing news. I found the problem. After reading over the documents above I decided to trace the F Terminal. At first everything looked fine. After an hour I was leaning over the car pulling my hair out of my head when I noticed that a tiny piece of one of the pins in the connect was sticking out the bottom and didn't look the same as all the rest. When I took apart the connector I realized that the pin was loose and that the wire looked fine from the outside but as I put the connect together the wire was actually moving out of the housing. I pulled the wire forward through the broken connector and put the pin directly into the other side so it was plugged in. Then slid the rest of the connector together. When I started the car 2 years of issues were gone! I can't thank you enough. Sometimes when your caught up in not being able to fix something you need an outside prospective. I can't thank you enough for giving it to me!
I just handed you the tool. You did the work.


Oooooooh – just lovely! I really like the collage look to this … hard to pull off sometimes. Well done with the DD challenge!!
Very elegant it's your colour combination and it shows well done.
Your card is so full of beautiful details, love verything about it!
Thank you so much for joining us at Dynamic Duos!


text:
Posted on March 21, 2016 March 24, 2016 by Penland
This Is a Photograph | Penland Gallery Exhibition
Chris McCaw, Heliograph 095, two unique gelatin silver paper negatives, 10 x 8 inches each. This image was created by exposing photo paper in a view camera for long enough to allow the sun to create a trail across the negative. This piece represents two solar exposures.
What possibilities do historic photographic processes offer to contemporary artists? What does it mean to make photographic images with chemically-sensitized and processed materials in the digital era? These are some of the questions raised by "This Is a Photograph: Exploring Contemporary Applications of Photographic Chemistry," the inaugural exhibition at the newly renovated and expanded Penland Gallery & Visitors Center. Curated by Brooklyn-based photographic artist and long-time Penland instructor Dan Estabrook, the exhibition not only reveals some of the arresting possibilities of these processes, it also brings work by world-class image makers to our community here in Western North Carolina.
Jerry Spagnoli, Glasses 3-3-12, daguerreotype, 14 x 11 inches. A daguerreotype is an image created on a silver surface that has been polished to a mirror finish and then sensitized with fuming iodine and bromine. Dating to 1839, it was the first widely-used photographic process.
"This Is a Photograph" displays work by twenty-three artists experimenting with a variety of processes and materials in ways that frequently have little to do with their historic antecedents: tintype images made on found metal objects, large daguerreotypes that look almost holographic, images created by painting directly onto photo paper with chemicals, and images made by igniting gunpowder that had been sprinkled directly onto photo paper, to name a few. As Penland Gallery Director Kathryn Gremley describes, "handmade images created through the complex alchemy of light and chemistry are the common ground of the artists invited by Estabrook for this exhibition."
"This Is a Photograph" opens on March 22, 2016. The gallery will celebrate with a public reception on Saturday, March 26 from 4:30-6:30 p.m at which Dan Estabrook and some of the artists will be present. The exhibition will be on display through May 1.
"This Is a Photograph" features the following artists: David Emitt Adams, Christina Z. Anderson, John Brill, Christopher Colville, Bridget Conn, Danielle Ezzo, Jesseca Ferguson, Alida Fish, Adam Fuss, Mercedes Jelinek, Richard Learoyd, Vera Lutter, Sally Mann, Chris McCaw, Sibylle Peretti, Andreas Rentsch, Holly Roberts, Mariah Robertson, Alison Rossiter, Brea Souders, Jerry Spagnoli, Bettina Speckner, Brian Taylor
Read Dan Estabrook's essay on the show below, and you can see images of all the work in the show on the Penland Gallery website.
Adam Fuss, Untitled 2006, unique cibachrome photogram, 30 x 40 inches (courtesy of Cheim and Read, NY). This image was created by exposing color photographic paper through a transparent tank of colored water (with a baby in it).
One year ago, I was here at Penland teaching a workshop called "Photography in Reverse," in which the students and I worked backward through the entire history of photography, stopping at key moments to experiment, play, and think about the nature of each technology. Starting with our smartphones and handheld devices—the very definition of today's tech—we began to ask ourselves how photography has changed at this critical moment, now that almost all our daily photographic usage is created and printed digitally. At our first step backward in time, with the earliest digital cameras, we learned something crucial: although photography is becoming purely digital, like much else in our life today, we still live in a physical world, and there are artists who will always want to make physical things.
Christopher Colville, Dark Horizon 41, gunpowder generated gelatin silver print; unique print, 8 x 6 inches. This image was created by igniting gunpowder in the presence of photographic paper.
We had to scramble to find the right cords and batteries and software so we could use some early digital cameras from 2001, and it became evident how much harder it was to work with the obsolete technology of 5 or 15 years ago than with the processes of 150 years ago. Most of our computers now can't run the first version of Photoshop (ca. 1990) or read early Photo CDs or Zip Drives. Even the standard color snapshot is being discontinued, since the machines required to make and develop color films are disappearing for good. The history of photography, like the history of technology in general, seems to suggest that every new system or process is an advancement on the last, making all older forms obsolete. And yet for every technique that has been pronounced dead, there seems to be an artist ready to explore its particular expressive qualities. After all, decades after the invention of mass-produced ceramics, people still want to throw beautiful pots. The artists in this exhibition are each exploring the possibilities of physical and chemical photography to pursue their own contemporary aims, very much in the here and now.
Some are finding a wealth of new beauty in the simplicity of the photographic act—a permanent mark made by the meeting of light and chemistry. Others are deeply engaged with history, in how we look backward from the present or forward to the years ahead. Still others have realized how much can be revealed in the life of a physical photographic object. Any technology that can still be used by artists, whether it's something that can be handmade or something produced from saved and scavenged machines, is going to have an ongoing parallel history through the work of these artists, not just as a period relic but as a technology carried along into the present with new developments and new meaning for the future.
A decade from now it will likely be easier to make a daguerreotype than to use the iPhone you bought in 2016; in 100 years that will be even more true. In the meantime, there will be artists like these to involve us in the material world in which we live, and to expand the possibilities of just what a photograph is.
Dan Estabrook | Studio Artist | Penland Instructor
Sally Mann, Untitled (Self-Portraits), 2012, unique collodion wet-plate positives on metal with sandarac varnish, 9 parts, 10 x 8 inches each (courtesy of Edwynn Houk Gallery, NY). These self-portraits were made using the traditional tintype technique, which involves pouring a liquid emulsion onto a metal plate and then exposing it before it has completely dried.
Alida Fish, Winter Leaves, archival pigment print transferred onto oxidized aluminum, 24 x 20 inches. Alida creates patterns of oxidation on aluminum sheets and then transfers digitally-printed photographs onto the metal surface.
Categories: Events, Penland Gallery, Penland People
Tags: Dan Estabrook, exhibitions, Penland, Penland Gallery, penland photography, Penland School of Crafts, photography
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