text:
Q: Find an existing messaging group when given the potential members I have an application where users can create messaging groups. MessageGroups have members through MessageMemberships. MessageMemberships belongs to a 'profile', which is polymorphic due to their being different types of 'profiles' in the db.
MessageGroup
class MessageGroup < ApplicationRecord
  has_many :message_memberships, dependent: :destroy
  has_many :coach_profiles, through: :message_memberships, source: :profile, source_type: "CoachProfile"
  has_many :parent_profiles, through: :message_memberships, source: :profile, source_type: "ParentProfile"
  has_many :customers, through: :message_memberships, source: :profile, source_type: "Customer"
end

MessageMembership
class MessageMembership < ApplicationRecord
  belongs_to :message_group
  belongs_to :profile, polymorphic: true
end

In my UI, I'd like to be able to first query to see if a messaging group exists with exactly x members so I can use that, rather than creating an entirely new messaging group (similar to how Slack or iMessages will find you an existing thread).
How would you go about querying that?

A: The code (not tested) below assumes:

*

*You have (or can add) a message_memberships_count counter_cache column to the message_groups table. (and maybe adding an index to the message_memberships_count column to speed up the query)

*You have proper unique indexing in the message_memberships table that will prevent a profile from being added to the same message_group multiple times

How it works:

*

*There is a loop that will do multiple inner joins on the same table to ensure that the association exists for each profile

*The query will then check that the total number of members in the group is equal to the number of profiles

class MessageGroup < ApplicationRecord
 ...
 def self.for_profiles(profiles)
    query = "SELECT \"message_groups\".* FROM \"message_groups\""

    profiles.each do |profile|
      klass = profile.class.name
      # provide an alias to the table to prevent `PG::DuplicateAlias: ERROR
      table_alias = "message_memberships_#{Digest::SHA1.hexdigest("#{klass}_#{profile.id}")[0..6]}"
      query += " INNER JOIN \"message_memberships\" \"#{table_alias}\" ON \"#{table_alias}\".\"message_group_id\" = \"message_groups\".\"id\" AND \"#{table_alias}\".\"profile_type\" = #{klass} AND \"#{table_alias}\".\"profile_id\" = #{profile.id}"
    end

    query += " where \"message_groups\".\"message_memberships_count\" = #{profiles.length}"

    self.find_by_sql(query)
  end

end

A: Based on @AbM's answer I arrived at the following. This has the same assumptions as the previous answer, counter cache and unique indexing should be in place.
  def self.find_direct_with_profiles!(profiles)
    # Not present, some authorization checks that may raise (hence the bang method name)

    # Loop through the profiles and join them all together so we get a join that contains
    # all the data we need in order to filter it down
    join = ""
    conditions = ""
    profiles.each_with_index do |profile, index|
      klass = profile.class.name
      # provide an alias to the table to prevent `PG::DuplicateAlias: ERROR
      table_alias = "message_memberships_#{Digest::SHA1.hexdigest("#{klass}_#{profile.id}")[0..6]}"
      join += " INNER JOIN \"message_memberships\" \"#{table_alias}\" ON \"#{table_alias}\".\"message_group_id\" = \"message_groups\".\"id\""
      condition_join = index == 0 ? 'where' : ' and'
      conditions += "#{condition_join} \"#{table_alias}\".\"profile_type\" = '#{klass}' and \"#{table_alias}\".\"profile_id\" = #{profile.id}"
    end

    # Add one
    size_conditional = " and \"message_groups\".\"message_memberships_count\" = #{profiles.size}"
    # Add any other conditions you may need
    conditions += "#{size_conditional}"
    query = "SELECT \"message_groups\".* FROM \"message_groups\" #{join} #{conditions}"

    # find_by_sql returns an array with hydrated models from the select statement. In this case I am just grabbing the first one to match other finder active record method conventions
    self.find_by_sql(query).first
  end


In another stupid comment showing her intolerance of and prejudice for us Southern folks, Ocasio-Cortez demands that everyone should be covered by medicare, "because children in Alabama, she say's, have ringworm and no one there to treat them." Whoa, I didn't know!
She could as easily have said "that children in New York (upstate, of course) have ringworm and there's no one up there to treat them," couldn't she? It's not because she a gifted bartender, or a cute dancer or that she's Puerto Rican that she says such intolerantly ignorant things. No, it's because she was taught to believe these thing in the socialist brainwashing centers called US colleges and universities. My guess is she has never visited the South and probably never will except under protective guard. Oh darn, I forget.The Mason-Dixon line does put Maryland in the South, doesn't it? That aside, she'll only say, her comment was "taken out of context." Naw!
I cannot tell you how unimpressed I am with the breed of new leaders emerging from their scholastic cocoon's to impress us with how smart they think they are. But, I have to ask, why do educated illiterates like comrade AOC, think that ringworm is a contagion only in Alabama? Is it because she believes Alabama is in the South and Southerners are therefore, mostly ignorant bible thumping, gun toting white people prone to illness, scurvy, scabies, hate, malnutrition, rednecks and ringworm, end up in the KKK and haven't made amends since the Civil War yet?
To understand the changes going on we must realize the political terminology has been changed and we didn't see it coming. In today's new society they are not communists, but environmentalists and the Green New Deal is their catechism. Environmentalism is the new Marxism and it demands that industry should move swiftly away from fossil fuels that pollute the earth, and causes ringworm in Alabama, and embrace their cockamamie ideas that cost more to install and run than simply scrubbing coal dust before it enters the atmosphere. Simple!
The unvarnished truth is socialism, even in the guise of environmentalism, always fails. For example, two highly touted environmentalist programs, initiated and operated at great costs, i.e.: the California high speed train to nowhere and the giant windmill eagle blenders polluting the visual as well as the natural habitat of America's open spaces, are already becoming liabilities to the investors that installed them and the environmental who demanded them.
California's white elephant, the train to no where, is just that. Gov. Gavin Newsom, of the Great Socialist State of California, after having left San Francisco, where he was mayor, in a feces filled, drug infested shambles, reckons he needs more funds to impose more socialist programs on all Californians, so he abandoned AOC's dream, the High Speed train as a financial liability leaving all American tax payers to hold the bag. Or, maybe not. Donald Trump now demands the Federal Government's investment, all of it, be returned to the US Treasury, forthwith. You know what? He'll get it too because Gov. Newson is a stupid Socialist who clings to the "we meant well" and "it feels good" mantra all Socialists use when they fail.
The windmills, now polluting the western prairies where I go every season, cost more in power to keep 'em from freezing in the bitter cold months of winter, than they produce in usable energy. Residents of Falmouth, Mass., conned into accepting the wind power scheme, are now turning them off for health reason as well as for inefficiency. Who's going to pay to take 'em down. The western plains are littered with this junk and the costs of inefficiency are passed down to the rate payers and eventually to all Americans. Loser should pay the clean up costs.


Fine Line is the sequel to Woodcutter's Revival. Was our nation losing its innocence at the end of the gilded Age? Fortunes were made in steel. oil, raliroads and banking. Follow the characters as they discern the changing times. As they find love, happiness, purpose and a home, find yourself immersed in this engaging plot. Discover the fine line between passion and ptovidence, physical, metaphysical and spirituyal reality.


text:
12 TV Execs To Watch
Nexstar Live Streams
B+C MCN December Issue
FCC Milks Mancow for $300 Thou
By John Eggerton
Emmis has paid the FCC $300,000 to settle a series of indecency complaints against its Mancow Morning Madhouse program and WKQX(FM) Chicago stretching back to January 2002. It has conceded that some of the material was indecent and has promised to implement a "companywide compliance plan" to make sure it doesn't happen again.
The incidents had involved proposed fines of a total of $42,000 for three incidents, one involving a telephone conversation with a porn star, one involving airing of a song entitled "Smell My Finger," and a third that involved innuendo, though no graphic or explicit references, regarding various sex practices.
Emmis had originally challenged all the notices of apparent liability, but has since decided to pay up, clean the slate, and move on. If it looks like Emmis got a raw deal, paying $300,000 to settle $42,000 in proposed fines, looks can be deceiving. The consent decree also settles all enforcement bureau investigations and complaints.
That includes at least a dozen complaints in the pipeline, said an Emmis spokeswoman, mostly from one complainant in Chicago, again against Mancow. The spokeswoman would not identify which of the forfeitures or pending complaints Emmis conceded were indecent, but she praised the DJ's ongoing efforts to keep the show "within the lines," saying he was doing a "great job."
Actually, the number of potential complaints expunged is 27, according to an FCC staffer familiar with the complaints. In addition to the three forfeitures, there were six complaints that hadn't been acted on, plus a request to reinstate 21 previously denied complaints.
"Earlier this year we adopted an aggressive policy to ensure that Emmis provides quality, compelling, on-air content that conforms to decency standards," Emmis President and CEO Jeff Smulyan said of the settlement. "We announced a zero tolerance policy and are taking extraordinary steps to educate our on-air employees and program directors. The consent decree settles all pending indecency-related issues, and allows us to move forward."
FCC Commissioner Michael Copps supported the settlement of the forfeiture order, but was not happy that Emmis was off the hook for outstanding complaints and investigations. ""The totality of a broadcaster's record is pertinent and should be considered when licenses are renewed. Today's decision takes an entire part of the record off the table.
John Eggerton
Contributing editor John Eggerton has been an editor and/or writer on media regulation, legislation and policy for over four decades, including covering the FCC, FTC, Congress, the major media trade associations, and the federal courts. In addition to Multichannel News and Broadcasting + Cable, his work has appeared in Radio World, TV Technology, TV Fax, This Week in Consumer Electronics, Variety and the Encyclopedia Britannica.
Peacock Losses, Hurricane Ian Hurt Comcast 4Q Earnings
Vizio Adds Features To Help Viewers Find Streaming Content
Groups Pan Meta Plan To Reinstate Trump Accounts
Matt Borek Named Managing Director Of Scripps' Florida 24 News Network
CBS Orders More 'Bob Hearts Abishola'

redpajama_set_name: RedPajamaCommonCrawl


I bought these for $40 a couple of weeks ago (they're super cute!) and now all Clearance is an extra 40% off taking these down to $23! (Originally $180)! Use code EXTRA40 for an extra 40% off. Shipping is free if you have shoprunner.


PCR #214. (Vol. 5, No. 18) This edition is for the week of April 26--May 2.
Hello, gang! A couple notes, a real American hero falls and more on the Beatles. Shall we begin?
Happy to announce that this week I have finalized interviews with actor/director Keith Gordon and actor/writer Robert Wuhl. Look for them soon.
After 9/11, it was thought of as bad taste to refer to an athlete as a hero. However, that word is more then appropriate when speaking of former Arizona Cardinal Pat Tillman, who died this week after a firefight in Afghanistan. After the attacks on America on 9/11, Tillman and his brother, a professional baseball player, informed their respective teams that they were enlisting in the US Army Ranger Corps. At the time of his enlistment, Tillman turned down a contract for $3.6 million to serve his country for a mere $18,000 a year. It was something he felt strongly about and he made the ultimate sacrifice for the country he loved. Pat Tillman was indeed a true American hero.
WHAT ABOUT "THE NIGHT CHICAGO DIED"?
April 26, 1964 - Making their first live appearance in Britain in 15 weeks, the group appears at the New Musical Express Annual Poll-Winners' All Star Concert. They play "She Loves You," "You Can't Do That," "Twist and Shout," "Long Tall Sally" and "Can't Buy Me Love" before an enthusiastic 10,000 fans.
Well, that's it for now. Have a great week. I want everybody to Wang Chung tonight. See ya!


text:
Hotel De Los Volcanes
Puerto Varas, Puerto Montt
Headline Near Puerto Varas Casino Rooms Treat yourself to a stay in one of the 50 guestrooms, featuring private spa tubs and flat-screen televisions. Cable programming and DVD players are provided for your entertainment, while complimentary wireless Internet access keeps you connected. Private bathrooms with separate bathtubs and showers feature deep soaking bathtubs and complimentary toiletries. Conveniences include phones, as well as laptop-compatible safes and desks. Amenities Relax and unwind with body treatments and facials. You can take advantage of recreational amenities such as a health club, an indoor pool, and a spa tub. Additional amenities at this hotel include complimentary wireless Internet access, concierge services, and a hair salon. Location A stay at Hotel Dreams de los Volcanes -Puerto Varas places you in the heart of Puerto Varas, steps from Lake Llanquihue and Puerto Varas Casino. This 4-star hotel is 0.1 mi (0.1 km) from Puerto Varas Beach and 0.2 mi (0.3 km) from Puerto Varas Plaza de Armas. Dining Grab a bite to eat at one of the hotel's 2 restaurants, or stay in and take advantage of the 24-hour room service. Snacks are also available at the coffee shop/café. Quench your thirst with your favorite drink at the bar/lounge. A complimentary buffet breakfast is served daily from 7:00 AM to 10:30 AM. Attractions Distances are displayed to the nearest 0.1 mile and kilometer.
Lake Llanquihue - 0.1 km / 0.1 mi
Puerto Varas Casino - 0.1 km / 0.1 mi
Puerto Varas Plaza de Armas - 0.1 km / 0.1 mi
Puerto Varas Beach - 0.2 km / 0.1 mi
Church of the Sacred Heart - 0.7 km / 0.5 mi
Gotschlich House - 0.8 km / 0.5 mi
Pablo Fierro Museum - 0.8 km / 0.5 mi
Yunge House - 0.9 km / 0.5 mi
Opitz House - 0.9 km / 0.6 mi
Kuschel House - 0.9 km / 0.6 mi
Parque Philippi - 0.9 km / 0.6 mi
Cerro Calvario - 1.1 km / 0.7 mi
Raddatz House - 1.7 km / 1 mi
Venado Beach - 18.6 km / 11.6 mi
Colonizacion Alemana Monument - 20.3 km / 12.6 mi
Punta Arenas (PUQ-Carlos Ibanez Del Campo Intl.) - 2,133.5 km / 1,325.7 mi
Puerto Montt (PMC-Tepual) - 22 km / 13.7 mi
The preferred airport for Hotel Dreams de los Volcanes -Puerto Varas is Punta Arenas (PUQ-Carlos Ibanez Del Campo Intl.).
BusinessAmenities Featured amenities include a business center, complimentary newspapers in the lobby, and dry cleaning/laundry services. Planning an event in Puerto Varas? This hotel has 7965 square feet (740 square meters) of space consisting of conference space and a meeting room. Guests may use a roundtrip airport shuttle for a surcharge, and free self parking is available onsite. Renovations The following facilities or services will be unavailable from September 9 2020 to December 31 2020 (dates subject to change):


The Hopkins Activity Center is a community facility with a gym, kitchen and meeting rooms used by residents for active or passive, large or small activities.
The Activity Center's mission is to provide pathways to "Experience the Upside of Aging." It is a gathering place for mature adults to participate in recreational, social, educational, fitness and volunteer activities.
Add or renew a membership or register for activities on the Hopkins Activity Center's new online portal.
View current and past issues of News and Notes for Activity Center programs.
To learn more, view the Activity Center Rental Page.


We provide bespoke joinery for kitchen fitting, bathrooms, all home improvements, sash and case windows plus more. We are based in Edinburgh but also serve wider Scotland. Established in 1970, we have over 48 years of expertise undertaking all types of residential and commercial joinery and building projects from day-to-day repairs up to long term contract work. Please take a look around our website and get in touch for a free estimate. We look forward to hearing from you.
Here at B&M Joiners & Building Services, we offer a wide range of expertise covering all trades for the Edinburgh area. From home improvements to commercial work, all joinery, kitchen and bathroom fitting plus sash and case window repairs and a whole lot more. Please visit our Services page for further details.
I've used this company on several occasions. Their work is always completed to a very high standard. The coordination of different services is very smooth. The workmen are always punctual and tidy. I thoroughly recommend them.
In our Blog you can discover examples of our work, case studies, B&M Joiners latest news plus features on our passion for creating and delivering craftsmanship for a wide range of both residential and commercial clients across Edinburgh and Scotland.
We are delighted to announce that B&M Joiners & Building Services have been approved by The Edinburgh Trusted Trader Program. The scheme, founded in 2015, is the premium "go to"
Sash & Case Windows Direct.Com is our sister company offering high quality services for repairing, restoring and replacing sash and case windows in Edinburgh and across Scotland. To find out more, please click on the link below to view the dedicated website.


That's right - you can now make a duplicate of your Google Site. This is great for numerous reasons, including being able to experiment with a feature on your own site and see what the results are. Another reason that comes to mind right away is being able to share your site with someone so they can see the ins and out of your site and how you created it.
Embedded below is a 55 second YouTube tutorial showing how to duplicate your site.
Please note that this feature is being rolled out, so if you do not see the ability to duplicate, please be patient. My personal account has this feature while my work one does not.


text:
Q: Correct allocate and free memory for arrays in C++ I'm dealing with  dynamic arrays. The function empty_matrix() creates a new array, representing a matrix. delete_matrix() frees all memory, allocated for the matrix.
Do I get a memory leak in function example() if I call add(add(a, b), c)? What will happen to the memory allocated in the function add(...)? Do I have to free it? Where should I do it?
 matrix empty_matrix(int dim) {
 matrix m;
 m.dim = dim;
 m.data = new int*[dim];
 for (int i = 0; i < dim; i++)
  m.data[i] = new int[dim];

 return m;
}

void delete_matrix(matrix m) {
 for (int i = 0; i < dim; i++)
  delete [] m.data[i];
 delete [] m.data;
}

matrix add(matrix a, matrix b) {
 matrix c = empty_matrix(a.dim);
 for (int i = 0; i < a.dim; i++)
  for (int j = 0; j < a.dim; j++)
   c.data[i][j] = a.data[i][j] + b.data[i][j];

 return c;
}

void example() {
 matrix a = empty_matrix(100);
 matrix b = empty_matrix(100);
 matrix c = empty_matrix(100);

 // some modifications of a, b and c
 // ...

 matrix d = add(add(a, b), c);
 print_matrix(d);

 delete_matrix(a);
 delete_matrix(b);
 delete_matrix(c);
 delete_matrix(d);
}

A: What you should do is use Object Orientation/RAII. Your data member of matrix class should be private, and memory for it should be allocated in the constructor, and freed in the destructor. This way, you won't have to worry about memory leaks.
For example...
class matrix
{
public:
      typedef int element_type;
      matrix(int dimension)
          :data_(new element_type[dimension*dimension])
      {

      }
      //Do define your copy-constructor and assignment operators
      ~matrix()
      {
         delete [] data_;
      }

private:
      element_type* data_;
};

This all, of course, if this is homework. If it is not, then you should refrain from using arrays in this situation. Use std::vectors

A: Yes, you will have to free the result of any empty_matrix or add call using delete_matrix. You're leaking memory, because you're not freeing the matrix of the innermost add call.

A: You should have exactly one new for one delete and one new[] for one delete[] in your code. That's the most important principle.
Hence, if the function add creates a new matrix, it needs to be deleted somewhere.

Also, empty_matrix should be the constructor of the matrix class, and delete_matrix should be its destuctor.
You could also replace the data with a std::vector and your memory handling would be much more automated, relieving you from the necessity of counting news and deletes.

redpajama_set_name: RedPajamaStackExchange


text:
Home/POLITICS/I won't interfere with your work – AG to Special Prosecutor
I won't interfere with your work – AG to Special Prosecutor
Nana Bosompra Send an email September 1, 2021
The Minister of Justice and Attorney General, Godfred Dame, has served notice he would respect the independence of the Office of the Special Prosecutor and would not stand in his way in the fight against corruption.
"I pledge to you my intention not to interfere with your work and I will not do that at all. I recognise your independence and I will make sure all the help you require is given to you from recruitment of competent personnel to budgetary allocation," Dame said when Kissi Agyebeng, the special prosecutor paid a courtesy call on him.
On his part, Agyebeng said he is currently reviewing all cases before him and the strength of his staff.
"I have about nine workers including drivers and cleaners, one prosecutor on secondment from your office, one investigator on secondment from the police. I am currently reviewing all cases before the Office," he said.
Agyebeng also appealed for attractive salaries for his staff to motivate them to work effectively. "You can't fight corruption with poorly paid staff and staff on secondments, it will be a lost battle," he said.
Agyebeng said he plans having in his fold 250 staff by the end of January 2022.
The special prosecutor was sworn into office on 5 August 2021. He was approved unanimously, barely an hour after his vetting, by the appointments committee of Parliament on Friday 30 July 2021.
Making corruption unattractive
He has promised to clamp down on corruption.
"I am not naive to assume that I am coming to stop corruption," Agyebeng said at his vetting. "There's no way I can stop corruption. God Himself will not lay claim to that, but I am going to make corruption very costly to engage in."
He added: "First, I am going to institute what I call 'Pressure for Progress' and in this quest, there will be a systemic review of all public sector institutions and the development of integrity plans."
Source: Asaaseradio
Government is fully behind GNPC - Energy Minister
Multimedia Group to file suit against Kennedy Agyapong for defamation


text:
using System;
using System.Collections;
using System.Collections.Generic;
using UnityEngine;

public class Gate : ActivatedObject {
    Animator myAnimator;
    bool open = false;
    // Use this for initialization
    void Start () {
        myAnimator = GetComponent<Animator>();
	}

    public override void Activate()
    {
        if (!IsActivated)
		{
            isActivated = true;
			myAnimator.SetTrigger("Open");
		}
    }

    public override void Deactivate()
    {
		if (IsActivated)
		{
            isActivated = false;
			myAnimator.SetTrigger("Close");
		}
    }
}

redpajama_set_name: RedPajamaGithub


Amazing Custom Handmade Aphrodite Wall Scrolls written in Chinese or Japanese.
Buy a Chinese and/or Japanese Aphrodite wall scroll with here!
艾福洛迪特 is a common transliteration to Mandarin Chinese for the name Aphrodite.
This is the name Aphrodite written in Katakana (phonetic Japanese).
艾福洛迪特 is one of a couple ways the name Aphrodite, Goddess of Love is written in Chinese characters.


text:
Species: Blue whale
Code: B 246 (MICS catalogue)
Calf: 1, in 2018
Observed in the Estuary: every year since 1991, except 1992, 2000 and 2011
From the bulletin Portrait de baleines, August 27, 2015
Jawbreaker is a female blue whale well known to the Mingan Island Cetacean Study (MICS). Although she has been observed a number of times in the Estuary, she has never been seen in the Gulf of St. Lawrence. Jawbreaker is recognized by the white spot on the underside of her tail.
However, as she is not the only blue whale to show this trait, we must rely on other criteria such as the pigmentation pattern of her back to confirm her identity. Also, her dorsal fin is light in colour, almost shiny. Only 15-18% of blue whales raise their tails out of the water when they dive. Jawbreaker is one of those individuals. This season, numerous employees of the whale-watching industry and visitors were able to observe this behemoth off the coast of Les Escoumins. Rare and fragile, the blue whale is an endangered species, having been decimated by rampant hunting in the North Atlantic that ended in 1955. In the Marine Park, an observation distance of 400 m must be respected.
MICS has been studying blue whales in the St. Lawrence since 1979. In the Northwest Atlantic, from the northern Davis Strait (between Greenland and Canada) to Bermuda, along the east coast, its researchers have identified over 475 individuals. This year, a big breakthrough in blue whale research was made. An individual photo-ID'd in 1984 in the St. Lawrence was found 30 years later in the Azores and again in the St. Lawrence, in the Gaspé, in 2015. This first ever documented sighting of a blue whale migration on an east-west axis, between the Gulf of St. Lawrence and the eastern part of the North Atlantic puts things into perspective. Measuring 25 m long and travelling hundreds or even thousands of kilometres, these giants live on a completely different scale.
Blue whale data sheet
Author: Camille Bégin Marchand
Taken from the newsletter Portrait de baleines, July 25, 2013
Jawbreaker is one of the blue whales that is particularly fond of the St. Lawrence Estuary: although well known to MICS researchers, she has never been seen in the Gulf. She usually shows her tail when diving, a personality trait that she shares with approximately 15-18% of individuals of her species photographed in the St. Lawrence.
Since the beginning of summer, the GREMM has counted at least four different blue whales in the Marine Park, including Jawbreaker. The latter has been observed from time to time since June 25. Nomadic, blue whales are constantly on the move in search of better patches of krill, small planktonic crustaceans that congregate in immense "clouds" driven by currents, tides, and underwater relief.
Why "Jawbreaker"? It seems that the origin of her nickname has been lost over the years! After being consulted, the MICS team believes that there may be a connection with the hard candy "jawbreakers", or else with an aggressive behaviour that she might have shown toward other blue whales.
The blue whale is a species at risk; its Northwest Atlantic population is unknown, but it is considered unlikely that it exceeds 250 adults. Disturbance by boats may be detrimental to its feeding. In the Marine Park, these animals enjoy enhanced protection: boats are required to stay at least 400 m away. Outside of the Marine Park, member companies of the Eco-Whale Alliance have undertaken to abide by these same limits to better protect them.
Blue whale Jawbreaker in the Estuary in 2013 © GREMM


The 41st president will be buried Thursday at his presidential library and museum at Texas A&M University.
The administration justified the move by saying it wants to give states more control over their Medicaid programs.
They are all active white supremacists; two of them are violent felons.
"The staggering, urgent need is simply beyond the capacity of government alone."
"He just completely turned around my year."
"Did it occur to you that a child may open it?" Charlene Sumlin wrote.
The virtually non-existent state environmental regulations have made the aftermath of Hurricane Harvey far worse than it would be otherwise.
"I think it's fair to say that this is now the most extreme multi-day rain event in U.S. history."
The university, which had earlier acknowledged the organizer's free speech rights, has now stopped the rally because of its links to the Charlottesville violence.
The "adultification" of black girls leads to harsher disciplinary treatment, researchers found.
Bacteriophages -- viruses found in soil, water and human waste -- may be the cure in a post-antibiotic world.


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^ Antonen, Mel (September 2002). "Boston's Derek Lowe Driven To Gain Success". Baseball Digest (United States: Lakeside Publishing Co) 61 (9): pp. 24 - 26. ISSN 0005-609X. http://books.google.co.jp/books?id=5y0DAAAAMBAJ&pg=PA26#v=onepage&q&f=false 2010年6月25日閲覧。.


text:
Home » Distributed Artists » Currently Reading:
Chris Robinson Brotherhood
April 13, 2017 Distributed Artists No Comments
Ego takes a backseat to community in the CRB, where collaboration carries the day. Rather than coming into the studio with a collection of finished songs for this album as he had in the past, Chris Robinson would present the group with sketches—a verse and melody here, a chorus and chord progression there—and let the band follow its collective muse to bring the music to life, a process he likens to putting an engine into the chassis of an old race car. Robinson had been sitting on "Leave My Guitar Alone," for instance, for nearly 15 years, but only once he presented it to the rest of the band did it roar to life in a way that had eluded him for more than a decade.
"It's a group effort," says Robinson. "All it takes is one good, small idea, and then if everyone's focused and in the moment, a few hours later, you can have something that you realize you'll be playing for as long as you're making music. I think when everyone's aware that that's the sort of magic that we're looking for, then it happens naturally. More than any other session that I've ever been a part of, that's how all of these songs were done."
www.chrisrobinsonbrotherhood.com


text:
Wilson named Rookie of the Month
SPRINGFIELD, Mass. … The American Hockey League announced today that Milwaukee Admirals center Colin Wilson has been named the Reebok/AHL Rookie of the Month for January. In 17 games during a busy month, Wilson tallied seven goals and 13 assists for 20 points along with a plus-5 rating.
Wilson entered January with just five points in his first 16 AHL games, but by month's end he had cracked the league's top 20 among rookie scorers for the season. Wilson notched his first multiple-point game as a pro on Jan. 3 at Hamilton, registering a goal and two assists in a 6-4 loss to the Bulldogs. He had an assist in three straight games from Jan. 7-10, and then went off for five points (1g, 4a) in Milwaukee's 6-2 win at Manitoba on Jan. 16, at the time tying an AHL season high. Wilson wrapped up the month on a five-game scoring streak, including a goal and an assist on Jan. 31 vs. Hamilton.
In recognition of his achievement, Wilson will be presented with an etched crystal award prior to an upcoming Admirals home game.
Wilson, 20, was the seventh overall pick in the 2008 NHL Entry Draft by Nashville and currently sits with 10 goals and 15 assists for 25 points in 33 games with Milwaukee. Wilson began the season with the Predators and notched two points in 10 games, including his first career NHL goal on Oct. 21 at Boston. Born in Greenwich, Conn., while his dad Carey played for the New York Rangers, Wilson represented the United States at the 2008 and 2009 World Junior Championships. In 2008-09, he was a finalist for the Hobey Baker Award and helped Boston University capture an NCAA championship.
Other nominees for Reebok/AHL Rookie of the Month include Adirondack defenseman Joonas Lehtivuori, Albany center Chris Terry, Chicago defenseman Paul Postma, Hamilton defenseman P.K. Subban, Hartford goaltender Chad Johnson, Houston center Cody Almond, Lake Erie left wing Justin Mercier, Lowell defenseman Matt Taormina, Manchester center Corey Elkins, Manitoba right wing Sergei Shirokov, Norfolk goaltender Dustin Tokarski, Portland center Tyler Ennis, Rochester goaltender Alexander Salak, Rockford defenseman Brian Connelly, San Antonio center MacGregor Sharp, Syracuse center Tomas Kana, Texas center Travis Morin, Wilkes-Barre/Scranton forward Eric Tangradi and Worcester left wing Brandon Mashinter.


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Q: How to resolve case problems for non-english languages in django admin panel? I need to resolve problem with word endings in django admin panel. The language I'm using is russian (using utf-8 charset), so some problems occur, for example, there is a problem with the right endings on the "Add" button for some model names. The simplest thing I found is using jQuery to correct endings "on the fly", but this solution is too radical. Maybe there is a simple answer? Just don't want to dig again into deepness of django's sources...

A: If I understood the problem correctly, you should just add an appropriate attribute in the meta section of the class.
English example:
class Man(models.Model):
    [...your fields...]

    class Meta:
        verbose_name_plural = "men"

More info can be found in the documentation for Django model options


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Q: How to download maven dpendencies to a specific folder I have following as dependencies in pom.xml file,
    <dependencies>
        <dependency>
            <groupId>org.apache.cxf</groupId>
            <artifactId>cxf-rt-frontend-jaxws</artifactId>
        </dependency>
        <dependency>
            <groupId>org.apache.cxf</groupId>
            <artifactId>cxf-rt-transports-http</artifactId>
        </dependency>
        <dependency>
            <groupId>junit</groupId>
            <artifactId>junit</artifactId>
            <scope>test</scope>
        </dependency>
    </dependencies>

I want to save those downloaded jar files to a directory which I specified. What is the exact way?

A: Try this in the build POM section:
<plugin>
    <groupId>org.apache.maven.plugins</groupId>
    <artifactId>maven-dependency-plugin</artifactId>
    <version>2.1</version>
    <executions>
        <execution>
            <id>copy</id>
            <phase>process-resources</phase>
            <goals>
                <goal>copy</goal>
            </goals>
            <configuration>
                <artifactItems>
                    <artifactItem>
                        <groupId>[your.dep.group.id]</groupId>
                        <artifactId>[your.dep.artifact.id]</artifactId>
                        <version>[your.dep.version]</version>
                        <type>jar</type>
                        <overWrite>true</overWrite>
                        <outputDirectory>[your.output.dir]</outputDirectory>
                    </artifactItem>
                </artifactItems>
            </configuration>
        </execution>
    </executions>
</plugin>


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"I purchased 2 cases raspberry and blue medium for my daughter and I. Bold colours, look great, light weight, so easy to push! Good value"
"Absolutely love this case. It looks amazing, has the inbuilt lock, and most importantly for me, it's so light yet very durable. I have a medium size one and am planning on buying the smaller one for cabin baggage!"
"Great value. I am impressed by the substantial feel of the zip and lock. Only one journey so far but happy with it."
"cases seem good and strong. fast delivery"
"Convenient size ideal for our needs"
"I ordered my medium luggage on Friday night and on Tuesday it arrived.I like it and would recommend to anyone who wants to purchase a medium size luggage."
"This suitcase is just the right size and the colour is easily spotted, ideal."
"Bought this suitcase to match my cabin bag and v pleased with my purchase. The price (especially in the sale!) was great value for the quality of suitcase. Bright colour makes it easy to spot on airport carousel and the layout of packing both sides is plenty space for a couple weeks in the sun!"
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Colby College, founded in 1813, the twelve oldest liberal arts college in America, is located in Waterville, Maine, USA. Its fifty-seven major and thirty-two minors (its departments and programs_ cover the major branches of the sciences, social sciences and humanities. Colby has 180 full-time faculty and 1,850 students. Colby's history and government departments and its program in Science, Technology, and Society contribute to world understanding of nuclear issues. Course offerings include Soviet history, nuclear history, Cold War studies, history of science and technology, and environmental history. Paul Josephson is a leading specialist on the history of nuclear power with a focus on the US and former Soviet Union.
Josephson will be involved in HoNEST both in analyzing and evaluating materials concerning nuclear history and policy in post-Soviet spaces and in writing sections of major reports, research papers, and articles concerning both those spaces and the entirety of the project.


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Q: Allow search bots to crawl your sites without session IDs Google's Webmaster guidelines state

Allow search bots to crawl your sites without session IDs or arguments that track their path through the site. These techniques are useful for tracking individual user behavior, but the access pattern of bots is entirely different. Using these techniques may result in incomplete indexing of your site, as bots may not be able to eliminate URLs that look different but actually point to the same page.

My ASP.NET 1.1 site uses custom authentication/authorization and relies pretty heavily on session guids (similar to this approach). I'm worried that allowing non-session tracked traffic will either break my existing code or introduce security vulnerabilities.
What best practices are there for allowing non-session tracked bots to crawl a normally session tracked site? And are there any ways of detecting search bots other than inspecting the user agent (i don't want people to spoof themselves as googlebot to get around my session tracking)?

A: The correct way to detect bots is by host entry (Dns.GetHostEntry).  Some lame robots require you to track by ip address, but the popular ones generally don't.  Googlebot requests come from *.googlebot.com.  After you get the host entry, you should check in the IPHostEntry.AddressList to make sure it contains the original ip address.
Do not even look at the user agent when verifying robots.
See also http://googlewebmastercentral.blogspot.com/2006/09/how-to-verify-googlebot.html

A: First of all: We had some issues with simply stripping JSESSIONIDs from responses to known search engines. Most notably, creating a new session for each request caused OutOfMemoryErrors (while you're not using Java, keeping state for thousands of active sessions certainly is a problem for most or all servers/frameworks). This might be solved by reducing session timeout (for bot sessions only - if possible). So if you'd like to go down this path, be warned. And if you do, no need to do DNS lookups. You aren't protecting anything valuable here (compared to Google's First Click Free for instance). If somebody pretends to be a bot that should normally be fine.
Instead, I'd rather suggest to keep tracking sessions (using URL parameters as a fallback for cookies) and add a canonical link tag (<link rel="canonical" href="..." />, obviously without the session id itself) to each page. See "Make Google Ignore JSESSIONID" or an extensive video featuring Matt Cutts for discussion. Adding this tag isn't very intrusive and could possibly be considered good practice anyway. So basically you would end without any dedicated handling of search engine spiders - which certainly is a Good Thing (tm).

A: I believe, your approach to the problem is not quite right. You shouldn't rely on session tracking mechanism to decide on access rights, to log malicious users, to detect bots etc.

*

*If you don't want arbitrary users to access certain pages, you should use authentication and authorization. If arbitrary users are allowed to access the page at all, they should be allowed to do it without any session ID (as if it is the first page they visit) - so, the bots will also be able to crwal these pages without any problems.

*Malicious users, most likely, could circumvent your session tracking by not using (or tweaking) cookies, referers, URL parameters etc. So, session tracking could not be reliably used here, do just plain logging of any request with its originating IP. Later you could analyze the collected data to detect suspicious activity, try to find users with multiple IPs etc. These analysis is complex and should not be done at runtime.

*To detect bots, you could do a reverse DNS lookup on the collected IPs. Again, this could be done offline, so no performance penalty. Generally, the content of the page served should not depend whether the visitor is a bot or an unaunthenticated human user (search engines rightfully treat such behaviour as cheating).

A: If spoofing is your main concern, you're doing security wrong. You shouldn't give robots any more permissions than users, quite the opposite (hence why users get logins and robots get robots.txt).
If you're going to give someone special privileges without authentication, it is inherently open for spoofing. IPs can be spoofed. Server-Client communication can be spoofed. And so on.
If you rely on tracking cookies to analyse malicious behaviour, you need to fix that. It should be easy enough to get a good understanding without requesting that the malicious user identify him/herself.
IPs aren't a good substitute for authentication, but they are good enough for grouping if cookies aren't available. Besides, you should be using more reliable means (i.e. a combination of factors) in the first place.


Mountains of opportunity to inspire ideas, enrich lives, and create community.
The mission of the Ruby M. Sisson Memorial Library is to serve as a physical and virtual hub where residents of and visitors to Archuleta County can easily and effectively obtain intellectual and cultural resources, as well as organized and relevant training opportunities that enrich their lives, improve self-sufficiency and expand career options. To accomplish this mission, the Library provides open access to print and digital materials, a wide range of databases and information services, organized classes and workshops. The Library supports lifelong learning for all ages.
The general assembly hereby declares that it is the policy of this state, as part of its provision for public education, to promote the establishment and development of all types of publicly-supported free library service throughout the state to ensure equal access to information without regard to age, physical or mental health, place of residence, or economic status, to aid in the establishment and improvement of library programs, to improve and update the skills of persons employed in libraries through continuing education activities, and to promote and coordinate the sharing of resources among libraries in Colorado and the dissemination of information regarding the availability of library services.


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I often get asked how we do all the homesteading we do, while raising and homeschooling 5 kids. Families with kids go perfectly together with homesteading! I thought I would share some of the tips and tricks for how we accomplish it all and get the kids involved.
Read Part 1 – Birth to Six Years Old, by clicking here.
And Part 2 – Seven to Eleven Years Old, by clicking here.
Homesteading with teens has been so much fun! I love watching them take charge of something and go with it, bringing in new ideas that I hadn't even thought of and making everything even more productive and fun. For the first 12 years they were following, and then they were able to start branching out and leading in their own ways.
So what does homesteading with teens look like? As I discussed in the previous post in this series, towards the end of the 7-11 year old age range the kids begin to find their niche on the homestead – the things around the home and farm that they like the best and are interested in – and we encourage them in those directions. We also start to include them in decision-making and researching things that we need to learn about. Those things continue in a bigger way into the teen years.
Since our teenagers know how to do all the different tasks around the home and farm due to being with us through their younger years learning them, we pretty much let them focus just on their niche tasks from here on out. If we need help with something, it is all hands on deck (or whatever hands are available at the time) no matter what niche it is. But for the most part the teens get to do their specific thing. For example, Sunshine loves gardening, especially the herb garden. So last year I gave the whole project to her. She got to decide how much to plant, what to plant, where to plant it, when to plant it, how and when to water it, etc. And then she carried out her plans. In the process she also saw a way we could better use the container herb garden space and she made a plan for remodeling it, got permission to carry it out, and then did it on her own. We had BY FAR the most productive kitchen herb garden we have ever had on this property. And for the most part, when we were gardening, she got to work on that and rarely had to help us in the main garden because she had enough to do with her own project. There is less side-by-side and more on-their-own at this age. But there is more discussion as they bring their ideas to us and ask what we think. So the relationship still continues. And often WE are the ones being their "little helpers" to help them make their project ideas a reality.
We also bring a lot more of the economic aspects of homesteading to their attention and ask them to think it through and make decisions to make the homestead more productive. We include them in the bigger decision making and research learning projects as we decide to add or remove some aspect to or from the homestead based on finances and productivity. Our farm is not a hobby farm, per say, everything needs to be as productive as possible and we don't keep livestock that don't earn their keep – we just can't afford it. So bringing the teens in on the finances and helping them see the big picture helps them understand why we make the decisions we do and will help them when they need to make similar decisions in their adult life.
An example of this is that Little Miss (who is just below this age range) is pushing for us to get back the milk goats. So I had her put together the start-up costs and maintenance costs and then figure out milk production and how long it will take the goat(s) to "pay-off" their start up costs (while maintaining their maintenance costs) with the milk we get from them that we don't have to buy at the store or milk-share. It helped her see the costs and benefits of having the goat(s) so she had a more realistic view of whether or not that was a good decision for the family and farm.
Homesteading with teens is fun because they bring their own ideas to the table and can work independently and take responsibility for things that they find interesting and fun.
We have now discussed all ages of children, from birth to teens, and how our kids participate on our homestead. As you can see, homesteading with kids makes it all the more fun, and helps build strong family unity. It also teaches the kids many practical skills, along with the love of learning and how to learn and teach yourself, responsibility, respect of animals, understanding of food and the work involved in producing it, good character traits, and self-esteem. When done with relationship as the top priority, it is a beautiful thing.
Read Part 1 – Birth to 6 Years Old, by clicking here.
In part 2 we will be discussing the age range from 7 years old to 11 years old. These age ranges, of course, are variable depending on the child.
As I said in the previous post, the main focus is building a strong relationship with my children by keeping them with me as I work on the homestead. I am not leaving them in front of a screen while I work. I am not sending them to do the work for me. We are working together, side-by-side – building a relationship, making memories, learning, and having fun while we do.
This age range lends itself to some more independence and responsibility. Since my kids have been with me working around the home and farm since they were born, by the time they are 7 and older they are very capable of doing most anything around the place that is safe for their size. But we still work together as a team, just with less instruction from me. Constantly micro-managing and nagging a kid who already knows how to do something will just lead them to dislike helping and working around the homestead. Also, expecting too much and being too critical will squash them as well. We all make mistakes, there is no need to get all worked up about an honest mistake.
So what does this age range look like on a day-to-day basis? We are working as a team, but they have more independence and responsibility and less instruction. An example would be, "We need to weed the garden, which section do you want?" They already know how to weed, and don't need me teaching them, so I give them their choice of area and then we go to work. Me in my area, they in theirs, and we are chatting and singing and having fun as we go about our work. If they have a question I am right there and ready to answer. When we are doing something new I include them in the learning process with me. I share with them the article or book I am learning from and then we go and learn through experience together.
Little Miss, who is 11 years old, is my main helper with this incubation we are doing right now. She was much smaller the last time we did an incubation, so this is really her first time learning how to do it and handle it on her own. We knew I would be gone for a few days during the incubation and she would be responsible for the incubators while I was gone since grandma doesn't know how to do it. So to prepare for this, we started by checking the incubators together each day and then I would tell her what we need to do and why and show her how to do it. After the first week I started having her check them on her own once a day and report back to me what the status was and what she did with them. Then we check them together the other times that day. When we candled the eggs she was right next to me and helping me mark down how the eggs were doing. After the first few I stopped telling her which ones were fertile and infertile and instead asked her to tell me. When I was gone she did beautifully at keeping the humidity right throughout the day and checking on the incubators. And she felt proud of herself because I trusted her with an important job.
This age range is fun because you start to be able to let them do things on their own and see them feel good about a job well done.
Towards the end of this age range I also start letting them find their niche. At younger ages they participated in anything and everything I was doing – which was everything. But as they get older they start to see which parts of the home and farm are their favorites and I let them do more of those things and ask them to less of the things they don't like as much. This does not mean getting to do the fun things and ignore the not so fun things (ie feed the livestock but not have to clean up their poop). It means getting to pick the types of livestock they like best or areas of the garden they like best. For example, Little Miss loves chickens and milk goats. So as she gets older she can take on more and more responsibilities in those areas, while not working as much with other things that she doesn't have as much interest in. The great thing about a family as big as ours is that everyone seems to favor different things than everyone else. Sunshine would much rather spend her time working in the garden or with the sheep than with the chickens and goats, so the two girls like the opposite things. It is still important to me that each child learns how to do every different part of the farm, but as they get older they begin to be able to focus more on the things that interest them the most. And they are still working side by side with Mtn Man or I, for the most part, so we are continuing to build that relationship. They really like getting to be specific helpers for certain things. Like Little Miss being the milking helper and the incubator helper.
As they reach the end of this age range we also start including them in the decision making and research when we need to learn something new. For example, if Little Miss and I go to milk and notice something different with the goat's udder I will discuss it with her and ask her what she thinks might be going on and what we should do. Then we will go to the goat book and look it up together and read about it and then do what is needed to treat it together. This builds knowledge and confidence in them that will serve them their whole lives. It also builds problem solving skills. Knowing how to find the information you need and then put it into practice is an excellent life skill. And it leads them to start recognizing what needs to be done and occasionally doing it on their own when needed. They are proud to come and tell me that they noticed that something was wrong with one of the chickens so they caught her, saw that she had some string around her foot, removed it, and then came to tell me about it. I can then praise them for seeing the problem and taking care of it without help and they feel a self-confidence that can only be created by actually doing something you know deserves praise.
And of course, it is important that they are not tackling just any task on their own. They know when they need to come get help and when they can handle it on their own. They know the ram is off limits, that they are not allowed to catch the rooster and can't climb into the barn loft without an older sibling or parent, etc. They have a good respect for the dangers around the farm and the dangers of certain animals and are careful by this age to come get help when needed. They know these things because we have been working side by side with them for 11 years and they have seen the good and the bad – the safe and the dangerous. They have seen a rooster attack me and the bruise it left. They know they don't want to get hurt like that. And we are still close by to be sure they are making good choices – they aren't just given the run of the place constantly. They do get to go off and do a quick job on their own, but we are keeping tabs on them and making sure all is well.
Sometimes an emergency comes up and the confidence that has been built helps them to think fast and do their best to remedy the situation. The story that comes to mind is of the time that the goats got loose and were heading up into the forest. I heard the guard dog barking and sent Little Miss out to see what was up. Usually, when I send one of the kids to see what the dog is barking at they are out and back in within a minute or two to report what the issue is. But after about 2 minutes Little Miss had not reappeared and the dog was still going crazy. I was up to my elbows in meatloaf (isn't that always when these types of things happen?) so I sent Young Man out to see what was up. He took two steps out the back door and immediately came back in and yelled for me that the goats were out. I ran outside right on the heels of Young Man, hands covered with raw meatloaf. You need to understand that Little Miss was about 9 at the time and is the skinniest little thing you have ever seen. A stiff breeze will blow her all the way to Kansas. As I came out the back door I saw her and our bully of a nanny goat, Gretchen, making their way up the mountain. Little Miss had both arms wrapped around Gretchen's neck, holding on for dear life and digging her heels into the ground trying to stop the goat from moving forward. Gretchen, who outweighed her by an easy 100 lbs, was marching on up the mountainside to freedom and adventure. But Little Miss was not to be dissuaded, and she was slowing the goat down considerably. She pulled and wrestled that goat as Young Man and I ran towards them full speed, with me yelling "You are doing great! Don't let go!!!" Our other goat, Heidi, was still standing just outside the gate watching the whole thing. As soon as she saw Young Man heading her way she went right back into the barnyard as if to say "I TOLD you we weren't supposed to leave the yard, Gretchen!" I passed Young Man as he closed the gate behind Heidi and caught up with the still-wrestling Little Miss and Gretchen. I grabbed a hold of her and the two of us managed to get her turned around and back into the barnyard. We closed the gate and collapsed together in a heap outside the gate, completely out of breath. Then we started laughing hysterically together. If Little Miss hadn't latched on to that goat she would have been long gone by the time we got out there because she was eager to run full blast into the forest. Little Miss slowed her enough that we weren't out all day trying to get her back home. She wouldn't have had the confidence to do that if she hadn't been handling the goats with us all along with us teaching her and praising her for a job well done. And now she loves it when I tell that story to people because she knows she did something great and helpful.
Even if our kids never have their own homesteads or use these specific skills, they are building good character traits, a love of learning and the knowledge of how to be self-taught, and self-confidence that will spill over into whatever they do. And the relationships we all have from working together are strong and priceless.
I will finish up this series with Part 3 – 12 years old and up.


Reston is full of hidden gems--it's like a real life treasure hunt here! And the The Wharf is one of them. What makes The Wharf so unique? This community of townhomes surrounds a pair of ponds at the top of a hill AND the community overlooks Lake Audubon. Yes, this is a water lover's paradise.
Reston architect Michael Oxman conceived of The Wharf in 1977. In a 1977 architectural magazine review he said: "When I decided to become a townhouse developer for my own design, I looked first at some lakefront property. Trouble is, only 20 percent of the townhouses on those sites are actually on the water. Those sell quickly, then there is often a long period trying to sell the off-water homes." (AE Concepts in Wood Design, Jan-Feb 1978, p. 9). This is not a problem in The Wharf.
Most of The Wharf's townhomes have garages.
The Cluster was built between 1981 and 1986, and is located on Timberhead Court off Glade Drive on Lake Audubon. The 55 cedar-sided, multi-level 3- to 5-bedroom townhouses were designed and built to look like a New England fishing village. Most of the homes have garages.
What really makes The Wharf special, though, is the ponds. These delicately balanced living ecosystems are not runoff ponds like other Reston lakes. Two fresh water wells provide water to the ponds. So they are more similar to mountaintop lakes since they are not contaminated from fertilizer and waste running off with rainwater. You will find blue-gill sunfish and large mouth bass in a ratio of ten sunfish to one bass. The sunfish eat mosquito larvae, and the bass keep the sunfish population under control. And they're fun to watch.
In a place renowned for access to nature, The Wharf stands out. This is a neighborhood for people who love water and want to be close to it. This neighborhood is also close to the Walker Nature Education Center, Lake Audubon pool, and South Lakes Village Center.


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Gene Circuit Compartment on Nano-interface Facilitates Cascade Gene Expression.
07:00 EST 13th November 2019 | BioPortfolio
Home » Topics » Bioinformatics » Research » Gene Circuit Compartment on Nano-interface Facilitates Cascade Gene Expression.
Summary of "Gene Circuit Compartment on Nano-interface Facilitates Cascade Gene Expression."
Cellular genes that are functionally related to each other are usually confined in specialized subcellular compartments for efficient biochemical reactions. Construction of spatially controlled biosynthetic systems will facilitate the study of biological design principles. Herein, we fabricated a gene circuit compartment by co-anchoring two function-related genes on surface of gold nanoparticles, and investigated the compartment effect on cascade gene expression in a cell-free system. The gene circuit consisted of a T7 RNA polymerase (T7 RNAP) expression cassette as regulatory gene and a fluorescent protein expression cassette as regulated reporter gene. Both the expression cassettes were attached on a Y-shaped DNA nanostructure whose other two branches were mercapto-modified in order to steadily anchor the gene expression cassettes on the surface of gold nanoparticles. Experimental results demonstrated that both the yield and initial expression rate of the fluorescent reporter protein in the gene circuit compartment system were enhanced compared with those in free gene circuit system. Mechanism investigation revealed that the gene circuit compartment on nanoparticle made the regulatory gene and regulated reporter gene spatially proximal at nanoscale, thus effectively improving the transfer efficiency of the regulatory proteins (T7 RNAP) from regulatory genes to the regulated reporter genes in the compartments, and consequently, the biochemical reaction efficiency was significantly increased. This work not only provided a simplified model for rational molecular programming of genes circuit compartments on nano-interface, but also presented implications for the cellular structure-function relationship.
This article was published in the following journal.
Name: Journal of the American Chemical Society
PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/31721571
DOI: http://dx.doi.org/10.1021/jacs.9b11407
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Medical and Biotech [MESH] Definitions
Gene Dosage
The number of copies of a given gene present in the cell of an organism. An increase in gene dosage (by GENE DUPLICATION for example) can result in higher levels of gene product formation. GENE DOSAGE COMPENSATION mechanisms result in adjustments to the level GENE EXPRESSION when there are changes or differences in gene dosage.
The use of techniques that produce a functional MUTATION or an effect on GENE EXPRESSION of a specific gene of interest in order to identify the role or activity of the gene product of that gene.
Gene Knockout Techniques
Techniques to alter a gene sequence that result in an inactivated gene, or one in which the expression can be inactivated at a chosen time during development to study the loss of function of a gene.
The GENETIC RECOMBINATION of the parts of two or more GENES resulting in a gene with different or additional regulatory regions, or a new chimeric gene product. ONCOGENE FUSION includes an ONCOGENE as at least one of the fusion partners and such gene fusions are often detected in neoplastic cells and are transcribed into ONCOGENE FUSION PROTEINS. ARTIFICIAL GENE FUSION is carried out in vitro by RECOMBINANT DNA technology.
Sets of structured vocabularies used for describing and categorizing genes, and gene products by their molecular function, involvement in biological processes, and cellular location. These vocabularies and their associations to genes and gene products (Gene Ontology annotations) are generated and curated by the Gene Ontology Consortium.
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A relative newcomer to the academic world and initially associated with vocational training, translation studies is now firmly established as a vibrant research field in many if not most universities around the world. In their various modes and diverse manifestations, translation and interpreting have further engaged scholars located well beyond the boundaries of translation studies – including sociologists, media and film specialists, forensic linguists, literary scholars and political scientists. Special issues focusing on translation and interpreting continue to appear in leading international journals such as Comparative Literature Studies, Social Dynamics, The Yale Journal of Criticism, Journal of Pragmatics, Journal of Visual Culture, Language and Literature, Discourse, and Public Culture, among many others.
Alongside the growth of interest in translation among scholars from various disciplines in the humanities and sciences, interdisciplinarity has become a key goal for academic institutions everywhere. But what does interdisciplinarity mean, generally as well as more specifically, in the context of translation studies? Does it mean borrowing methods and theoretical models from one or more other disciplines to address a research question that is specific to translation studies? Or does it mean addressing a question that is broader than the concerns of a single discipline such as translation studies by drawing on the knowledge and methodologies elaborated in several disciplines? And how does each type of encounter impact translation studies as a borrowing or collaborating discipline?
Organised to launch the Baker Centre for Translation and Intercultural Studies at Shanghai Jiao Tong University, and as the first of a series of events and initiatives to be carried out under its aegis, this international conference will bring together established scholars as well as doctoral students and early career researchers to examine various facets and challenges of pursuing interdisciplinary research on translation and interpreting, and of defining translation studies as an 'interdiscipline'. Contributions that focus on the negotiation of different epistemologies of knowledge, theoretical models and research methodologies are welcome, as are those that present case studies in which insights from other disciplines are operationalized, critiqued and adapted.
Potential themes of interest include but are not restricted to the following:
Interdisciplinary models for researching translation and interpreting in new settings, such as digital platforms, social media, asylum hearings, refugee camps;
The adaptation of theoretical insights and models from sociology, cultural theory, media studies, linguistics and other disciplines to address research questions in translation studies;
Models of collaboration across the human and natural sciences and the potential contribution of translation studies in this context;
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Q: Autofill data from one tab to another under certain criteria I enter customer details onto an excel 2010 spreadsheet, ie customer name, date, description of order, amount of order etc. Sometimes, under certain criteria, the same or part of the same information needs to be added to another tab, ie tab2, on the same spreadsheet. I wanted to create an automated system to populate tab2, but cant figure out how.
I thought about creating another column in tab1, before all the customer information, which had a dropdown list option for yes/no when asked the question 'does it fit the criteria to add customer information to tab2'. This column would act as an alert, so that if i selected 'yes', then any customer information i enter in tab1 would then populate into tab2. But I dont know how to do this, can anyone help?

A: Without more detailed information I can't offer specific code advise.  But in general you can use VBA Worksheet_Change event to detect new data entered into sheet 1 (tab 1) and test if it meets some criteria.  If it does, execute some code to copy to sheet 2