If you own a property and you ever feel the need to demarcate it with a boundary or a fence and keep it out of trespassers or unwanted guests in the form of humans as well as animals, then security fencing might be the best option for you. There are various types of security fencing that you can choose and all of them will do the required job for you. These security fencings are mostly made of wood, stainless steel or metals. You can easily install them in your property to keep your house safe from intruders.
A fence, basically, is a structure that is used to enclose an area, which is typically and mostly outdoors. It usually consists of posts that are connected with rails or rods and netting. However, it is not the same as a wall which is used as a boundary or to demarcate a property. It differs from a wall in the very foundation as security fencing does not have a solid foundation along with its entire length.
The primary purpose of putting up a security fence is to procure the best security measurements. It is also used to demarcate your area or your property in a particular area. However, there are particular areas or facilities that need to be fenced by law as well as moral responsibility.
You can also install such security fencing in your industrial areas and factories to segregate high voltage machinery or electric equipment. Most of these include transformer stations and radiators that are surrounded by barbed wire security fences. Security fencing is also used in pastures and farms where breeding animals like bulls and stallions are kept, so as to keep them safe from the other animals and humans. Public areas like swimming pools also require fencing to protect such areas form children.
There are various types and styles of fencing that are available in the market and can be set up in a number of ways. Here are a few types of security fencing that are used for particularly specific purposes.
#1. Timber or Wooden Fencing: This type of security fencing is the most affordable and they are perfect for small residential areas or small-scale commercial level. Even though it is not as secure as the other types of security fencing and they are mostly known as decorative fencing. To keep your property safe, you must choose some metal fencing.
#2. Wooden Hoarding: This is another cost effective but a stronger and sturdier type of security fencing. This type of fencing is usually seen at construction sites or properties that are vacant. These security fences not only conceal the property or the activities within from the eyes of outsiders, but they are also strong enough to keep it protected from any kind of intruders or trespassers.
#3. Heras Fencing: This type of security fencing is temporary and the least costly when it comes to value for the product. Heras security fencing is usually used more in the private sector, at events or house parties. For example, if you want to organize your party in your backyard areas and you want to keep your guests away from your main building then you can install such temporary fencing.
#4. Metal Hoarding: Metal Hoarding is one of the strongest security fences available in the market. Even though it is costlier than most of the security fences, it can be used for a longer period of time, and it can also be reused.
After getting to know about the various types of security fencing, it is important to choose the right type according to your requirements. You need to keep the factor of cost effectiveness in your mind as well.


Review: Drobo 5C -- safe, expandable external storage - iPhone J.D.
A Drobo is a smart external hard drive. Instead of a case containing just a single hard drive, a Drobo contains multiple hard drives, with every bit of data stored on at least two drives. Your computer (PC or Mac) treats it like a single big hard drive, but because all data lives on two different drives (or three if you enable that setting, resulting in less overall usable space), any one drive can fail and you will not lose any data. It is the perfect way to get the advantage of an external hard drive (lots of storage at an affordable price) while also avoiding the #1 problem of all hard drives — the fact that, at some point, every hard drive will fail. It is just a matter of time. I've had a few external hard drives fail on me over the years, and after one died on me in 2015, I decided that enough was enough. I bought my first Drobo in 2015, and I've been a very happy Drobo user ever since.
A few weeks ago, Drobo sent me a free review unit of the latest generation of the basic model of the Drobo, called the Drobo 5C. (Note also that Drobo is a past sponsor of iPhone J.D.) I've been using this Drobo 5C for the last few weeks, and it works great. And as explained below, as a result of me making a stupid mistake, I even got a chance to see what happens to a Drobo 5C when a drive fails.
I'll start by talking about the features of the Drobo 5C that are unchanged from prior models. Like other Drobo models, the Drobo is a black box which holds multiple hard drives. Each drive has a light next to it, and so long as the light is green, you know that the drive is performing the way that it should. If you ever see a blinking red light, that means that a drive has died or otherwise failed. Your data is still protected on the other drives, but it is time to order a new drive from Amazon or your hard drive seller of choice.
I know from first-hand experience that drive failure is not just a theoretical concern. I have had multiple external hard drives fail on me over the last 15 years, and every time it happened, it caused a lot of stress for me as I look to see if I have a reasonably current backup. But with a Drobo, the backup occurs constantly and automatically, so you don't have to worry about it.
Protection has always been one of the core advantages of a Drobo. The other longstanding advantage is the ability to grow the size of your Drobo as your needs increase. For me, my iPhone is one of the big reasons that I have needed more storage. Ever since the iPhone 6s came out, I have turned on the option to record video in 4K. 4K is a little overkill for now — I don't even currently use a TV that can show 4K — but I know that I'll be looking at home movies of my kids that I shoot today for many decades in the future. I remember being excited on my 27th birthday to get a huge 27" TV, which I cannot even imagine using today when I have a widescreen 65" screen. Who knows what technology I'll be using 10-20 years from now, so I figure I might as well create and save the highest quality video files today.
Of course, 4K video requires more disk space to store the video files. And even if you don't need to store 4K video, we all have larger files to save than we did years ago. Although I use my Drobo connected to my home computer, if you want to use a Drobo for your law practice, you know how the number of files associated with your matters proliferate every year. As attorneys become more and more paperless, we are all getting more PDF files, more JPEG files, and even videos associated with our cases, all of which take up more space. You can be sure that whatever is enough space for you today won't be enough space in another year or two.
Fortunately, Drobo can easily grow as your needs grow. Blue lights along the bottom of the Drobo show you how much capacity you are currently using up, with one light for each 10%. When you get to 85% capacity, the green light next to a drive will turn yellow, a sign from Drobo that it is time to replace that drive with a larger hard drive. If you get to 95% capacity, the light turns red. It doesn't matter if the different drive bays have different size hard drives; Drobo will just use whatever you give it. So just get a larger drive and add it, and it is OK if some of the other drives are smaller. Best of all, you don't need to worry about manually moving files from an old drive to a new drive; Drobo takes care of all of that.
Thus, with a Drobo, you don't need to worry about planning for the future. The capacity of a Drobo grows as you need it to grow. Drobo even has a handy Capacity Calculator on its website so you can see how much usable space you get when you add different sized hard drives to the different bays in a Drobo.
I use Backblaze for online backup of all of the files on my home iMac. Because Backblaze also backs up (at no extra charge) all files on any devices connected via USB, one nice thing about my prior Drobo and this new Drobo 5C is that there is a copy of all of files in the cloud. If any one hard drive fails, the Drobo 5C will protect me. If the entire Drobo is destroyed — a disaster such as a house fire, an asteroid landing on my block, etc. — I still have an online back up of the multiple terabytes of data being stored on the Drobo 5C.
The basic model of the Drobo used to hold four drives, and that was the model that I previously used to hold all of the files that were too big to store on the iMac at my home, such as home movies and other large video files. The Drobo 5C holds five drives. The extra drive bay means that you have the ability to hold more data. Additionally, having an odd number of drive bays means that you can sometimes increase the total available space on your Drobo just by adding one additional drive; with a 4-bay Drobo, you often need to add two larger drives to take advantage of an increase in total usable capacity.
New to the Drobo 5C is a USB Type-C connector on the Drobo itself. The Drobo 5C comes with a standard USB to USB-C cable. Chances are, your current computer just has a regular USB connector, so that cable will work great for you. But in the future, more and more computers will moving away from USB towards USB-C. (If you currently use a very new computer, you may already have USB-C.) So this Drobo works with current computers, while being ready for the future.
In theory, USB-C can be twice as fast as USB 3.0. Because I don't have a USB-C port on my computer and thus I used my computer's USB connection, I didn't notice any speed increase with the 5C versus my older Drobo. But if you have a newer computer with USB-C, I would think that this connection would be faster. As noted above, the main advantage that I see to using USB-C is future-proofing.
Drobo comes with Drobo Dashboard software that you can use to work with your Drobo. In the Dashboard, you can see specific details on drive capacity and health, dim the lights if you find them to be too bright, format drives, etc. I rarely have the need to use this software because the great thing about Drobo is that it just works, and all that you really need to do is glance at the lights on the front of a Drobo to check that all is well. Nevertheless, it is nice to be able to check in the Dashboard just to see how everything is working. And the Drobo Dashboard software lets you update the software on the Drobo itself when updates are released.
If you haven't used a Drobo before, the Drobo 5C is a great place to start. But if you have an older model of the Drobo, it is easy to upgrade. As noted above, I bought a 4-bay Drobo back in 2015. Moving to a new Drobo is just about as easy as shutting down the former Drobo, taking out those drives and putting them in the new Drobo, and then turning on the new Drobo.
At least, it should be that easy for most folks. I made a mistake when I did my upgrade. I successfully installed three of the hard drives, but for my fourth hard drive I wasn't paying close enough attention and I didn't push the hard drive all the way in. Thus, when I started up the Drobo 5C, it thought that one of my hard drives had failed, and it warned me to replace that hard drive with a new one.
At first, I was upset at myself for own incompetence. It's not like pushing a hard drive all the way in is all that complicated. Sigh. But then I realized that there was a nice silver lining; I got my own live demo of what happens when a hard drive fails and I see a flashing red light on the front of the Drobo. I'll be honest, my first reaction was a small amount of panic. I don't want to lose any of my files, especially home movies of my kids when they were younger! But then I realized that the Drobo 5C was purring along, working the way that it was supposed to work. Once the Drobo 5C finished configuring itself with the three drives that it recognized, I ejected that fourth drive and then put it into a drive bay — correctly this time. The Drobo erased and formatted that fourth drive (just as if it were a brand new drive), and then moved data from the three drives back over to that fourth drive. Once again, I had a Drobo with four working drives all with green lights. Whew! Whenever I do have a real hard drive failure, it will be nice that I already had what amounted to a fire drill so that I can remain calm and know that there is nothing to worry about; the Drobo was made to deal with these sorts of problems.
To take advantage of the new fifth bay that didn't exist in my older Drobo, I purchased a 2 TB hard drive from Amazon to add to the four 1 TB hard drives that I had been using. The drive I purchased cost only $89, and it increased my total usable space from 2.72 TB to 3.63 TB.
There is something very freeing about knowing that you have a ton of storage connected to your computer. Take all the photos and HD video that you want with your iPhone; you'll always have enough space because it is easy to expand if you need to do so. And it is even better to know that these precious files are saved not on just a single hard drive, which can fail, but on multiple drives that all work together thanks to the magic of Drobo.
Click here to get a Drobo 5C on Amazon ($349).


Introducing myself and saying why am I here.
Well, First, the introduction. My name is Michael Angelo L?pez Marsign?. I am 13 years old. Saying hello to the server. First, let's start from the beginning. I saw some users that didn't had forum, so my idea was to put a post to make a forum for someone with .tk domain, and I did. After some posts, I received a PM. The subject was "i need one !!!!!" by HaZaRaS? (AKA [hs]handsom_sagar). The PM was actually that he had a forum and he wanted to make his forum a .tk domain, but that wasen't actually that I was thinking. But, I wanted to help HaZaRaS?. I replied with "You want me to create a forums for you? Another? I can make you one with SMF and .tk domain." And he replied "i have .smffy.com .. so how can i put .tk ??". Then I replied (this reply is why I am developer) "It is easy, but I must be administrator to get in administration area and apply an .tk domain." And it was actually right. But cutting the other PM's, the result was that he had to get to host to change domain and if he did not had host, he had to make a new forum. The whole thing is that this forum doesn't support a domain manager, but the forum I create, I put a domain manager that the domain can be edited easily. But I will be searching for information, is the now answer. Now, you know why I am here.
Re: Introducing myself and saying why am I here.


The harder people work during the day, the more they wish to relax and unwind in the evening or during weekends, and what better place to do this than at home? There is nothing more important for a close knit union of people who share the same living spaces than to enjoy some quality time around the dinner table at night. Then is when all problems can be left aside and the troubles of the everyday living are out of a sudden unimportant. This is perhaps one of the main reasons why so many homeowners dedicate a lot of time and put a lot of effort into creating the best possible environment for their families. Creating great conditions and a pleasant atmosphere in their kitchens will help all the family members or additional residents to enjoy a great time.
Every once in a while we spend money on new gadgets and high tech pieces of electronics, but how often do we take a moment to think about the fate of our kitchens? And how can we really know if we have the most modern kitchen design ideas or not? One must stop and wonder how modern and up to date their kitchen really is. Keep reading this article and you will find out!
How to make your kitchen look more welcoming?
On the one hand, modern kitchen design ideas are generated by modern day needs, meaning that the large number of appliances and impressive variety of domestic devices has generated an incredible number of changes. These changes need to be implemented if you want to call your kitchen a modern and up to date one. What this means, in plainer terms, is that we have acquired a large number of necessary appliances which are often the reason why our cooking areas look more industrial like than welcoming. The solution to this recurring problem can be to implement our idea of finding original or unconventional looking shapes the next time you purchase an electric device for your home.
The main benefit of a contemporary looking kitchen, other than its increased functionality, is to leave any guest passing through the door in awe of how amazing and breathtaking the ensile looks like. Hearing your friends ask questions regarding the mysterious purpose of each item in the room will bring you lots of satisfaction.
A modern decor idea can be to adopt the minimalist trend or avant-garde tendencies in design. Turn your kitchen into a piece of art and make your guests and close ones believe that they have stepped into a fine art gallery, rather than your personal kitchen. For this idea to work out, all you have to do is acquire built in electronics and incorporated pieces of equipment which are not visible to the naked eye. Also, use gorgeous furnishings such as shabby-chic furniture or French inspired home decor choices and furnishings.


High ceilings and an open fireplace create a welcoming ambience from the moment you enter.
High ceilings and an open fireplace create a welcoming ambience from the moment you enter. Timber floorboards transition to a sky-lit open living zone soaked in natural light. Banks of windows wraparound the main living area and embrace it in green garden views. Fretwork ceilings add beautiful detail and enhance the sense of space while navy features contrast with the crisp white kitchen.
Two bedrooms benefit from walk-in wardrobes and loft-style storage makes clever use of the high ceilings. There is a walk-in shower in the bright bathroom and a good-sized laundry. A full-width deck has ample room for alfresco dining and lounging, and there is an inground pool and level lawn for entertaining. Vehicle access through to the backyard allows for boat or caravan storage and there is a garden shed tucked behind mature citrus and easycare greenery.
Located around the corner from Asquith Public School and metres from St Patrick's Catholic School, this home is two minutes walk to Asquith station. High schools, Mils Oval, Coles and medical services are less than 10 minutes by foot.


The project will scale Redavia's "pay-as-you go" industrial solar power service to other remote businesses, particularly into the food processing sector. The project overcomes the barriers of high up-front costs and long off take agreements by offering SMEs a standard, modular, and redeployable solar farm on a flexible, pre-financed rental basis. This scaling will leverage Redavia's existing commercial, technology, and financing platforms. Target food processing sub-segments are coffee, tea, oilseeds and cashews, which suffer from frequent (up to 25% of the time) power outages and/or high-cost diesel-generator power. The power is expected to empower rural women particularly through mechanisation of food processing and creating higher value jobs.


text:
title: "Crystal cryocooling distorts conformational heterogeneity in a model Michaelis complex of DHFR"
authors: "**Keedy DA**, van den Bedem H, Sivak DA, Petsko GA, Ringe D, Wilson MA, **Fraser JS**."
journal: 'Structure'
pub_date: '2014-06-10'
image: '/static/img/pub/2014_keedy.jpg'
pmid: '24882744'
pmcid: 'PMC4082491'
pdf: 'http://cdn.fraserlab.com/publications/2014_keedy.pdf'
pdbs:
- 4PSS
- 4PST
- 4PSY
- 4PSZ
- 4PTJ
- 4P3Q
- 4P3R
- 4PTH
links:
- name: Wilson lab @ University of Nebraska-Lincoln
  url: https://redoxbiologycenter.unl.edu/markwilsonphd
- name: Petsko/Ringe lab @ Brandeis University
  url: http://www.bio.brandeis.edu/prlab/
- name: Paper Submission Celebration Photo
  url: https://twitter.com/fraser_lab/status/443233965017559040
- name: F1000 Evaluation
  url: http://f1000.com/prime/718430130
---

redpajama_set_name: RedPajamaGithub


Five hour day package (minimum of 2 riders).
We require payment to be made in full prior to your ride. We accept paypal (bitofhoperanch@msn.com), checks or cash. You can pay with a credit card through Paypal.
We can travel to Myakka State Park, Carleton Reserve, and Deer Creek Prairie for an additional fee.
Palma Sola Bay in Bradenton, Florida.
One hour ride at the beach/swimming (minimum of two riders).
Watch the video to see what it's like to be on a trail ride.
Please contact Suzanne (941-650-5414) for more information and to RSVP!


text:
/*
 * Создано в SharpDevelop.
 * Пользователь: admin
 * Дата: 07.07.2018
 * Время: 16:33
 *
 * Для изменения этого шаблона используйте меню "Инструменты | Параметры | Кодирование | Стандартные заголовки".
 */
using System;
using System.Collections.Generic;

namespace AI.MathMod.AlgorAnalise
{
    /// <summary>
    /// Проверка схожести двух базисов
    /// </summary>
    public class SimilarityOfBases
    {
        private readonly List<Vector> bases1 = new List<Vector>();
        private readonly List<Vector> bases2 = new List<Vector>();
        private readonly Vector sim, maxSim;

        /// <summary>
        /// Проверка схожести двух базисов
        /// </summary>
        /// <param name="bas1">Базис №1</param>
        /// <param name="bas2">Базис №2</param>
        public SimilarityOfBases(Matrix bas1, Matrix bas2)
        {
            bases1.AddRange(Matrix.GetColumns(bas1));
            bases2.AddRange(Matrix.GetColumns(bas2));
            sim = new Vector(bases1.Count);
            maxSim = new Vector(bases1.Count);
        }

        /// <summary>
        /// Вероятность что базисы не связаны (случайны)
        /// </summary>
        /// <returns></returns>
        public double ProbRandBasis()
        {

            int k = 0;
            double prob;

            while (bases1.Count > 0)
            {
                for (int i = 0; i < bases2.Count; i++)
                {
                    sim[i] = Math.Abs(Statistic.CorrelationCoefficient(bases1[0], bases2[i]));
                }

                maxSim[k++] = Statistic.MaximalValue(sim);

                try
                {
                    bases1.RemoveAt(0);
                    bases2.RemoveAt((int)sim.IndexValue(maxSim[k - 1]));
                }
                catch { }

            }

            prob = 1 - Statistic.ExpectedValue(maxSim);

            return prob;
        }

    }
}


Respectively, Amr Diab has achieved the World Music Awards twice, as best selling middle eastern singer in both his albums "Nour El Ain" in 1998 and "Aktar Wahed" in 2002. Also received a Platinum Award for the sales of "Nour El Ain". Visit the awards section in this site to know more about Amr's awards.
2004's summer witnessed, the release of "Leily Nehary" album, Diab gave his fans what they've been longing for, a dazzling hit which blew their minds off and topped their sensations. "Leily Nehary" according to the production company "Rotana" and the fans is to be one of the most successful albums out in the market. "Kammel Kalamak" was released by December 2005 after a long anticipation from Amr's fans, according to the big sales records, the album was worth the wait! The album contains 10 tracks representing a year and half of continuous studio efforts.


text:
Q: Problema de posicionamiento de cajas Esta es mi primer prueba que hago después de leer un poco, seguramente sera muy sencillo para la mayoria de ustedes. Mi problema es que estoy tratando de estructurar el encabezado de la pagina pero no me posiciona unas cajas de la manera que quiero.
Adjunto el codigo para que vean de que hablo. Quiero hacer que lo que esta en tonos de verde quede donde esta lo amarillo. El tema es que el contenedor de los botones 5 y 6 me los ubica justo seguido del contenedor de los botones 3 y 4, y no mas arriba como se ve.
Aguardo respuestas. Gracias!

<!DOCTYPE html>

    <meta charset="UTF-8">
    <meta name="viewport" content="width=device-width, initial-scale=1.0">
    <meta http-equiv="X-UA-Compatible" content="ie=edge">
    <title>Esquema de prueba</title>

Nombre del Sitio

Boton 1

Boton 2

Logo

Seccion deslizable

Boton 3

Boton 4

Boton 5

Boton 6

Nombre Usuario

redpajama_set_name: RedPajamaStackExchange


These days, most of us have a smartphone or an iPad. It's simply become common place. What I've realized over the past few years as an Overland Park Chiropractor is that the neck pain, headaches, and back pain related to using these devices have also become commonplace.
Dr. Fishman who coined the phrase "text-neck" describes this condition as "an overuse syndrome where you have your head hung forward and down, looking at your device for extended periods of time." Yup, that about sums it up!
Most of my chiropractic patients don't make the connection between looking down at their devices all day and their neck pain because of the small muscles we use during the motion. That's a huge mistake to make because the impact of improperly using those muscles daily has lasting effects on our bodies and usually results in chronic neck pain, headaches, and back pain.
We do this motion all day and every day, which labels it as a repetitive action. This repetition puts our spine in the wrong posture many times throughout the day; therefore, neck pain is bound to be the result. Our head alone weighs approximately 8 to ten pounds. For our neck to be able to support such weight, it must be in the correct neutral posture which is having your ears over your shoulders for most of your waking hours. Texting brings our head forward and out of correct neutral alignment, each and every time we text; which for some of us is quite a bit!
Most of my chiropractic patients with "text-neck" suffer from neck pain and chronic headaches, but over time it can also begin to change the overall curvature of our spine especially as our kids being using these devices younger and younger.
What can we do to prevent Text-Neck?
– Strengthen your neck muscles & your upper back muscles.
– Bring the device up to a move neutral level.
– Make sure to stretch those muscles you are using.
– Stop using your devices as much!
– And of course maintaining proper spinal alignment is critical in preventing this problem!
Chiropractors are experts at helping your body maintain proper alignment or fixing it if it's causing you pain. If you already have a chiropractor make sure to have this conversation her on your next visit. If you don't have an Overland Park Chiropractor give me a call: 913-681-7757!


Central Hall is a unique and historic building, centrally positioned across the road from Westminster Abbey and Houses of Parliament and is a great place to have conferences and meetings.
Within these walls operates a conference and exhibition center, a concert hall, an art gallery, serviced offices, a Methodist church, a tourist attraction and a public café.
This affordable, versatile, venue offers organisers fifteen various size meeting rooms with natural day light and black out facilities, plus numerous other spaces for syndicate rooms, offices, exhibitions and dinners.
Our dedicated event managers will look after every aspect of your requirements. We can help you with theming or entertainment and we offer the latest audiovisual technology with qualified technicians. Central Hall offers a delicious catering selection and we will happily design a menu to suit your particular event.
Leanne is a real asset to your organisation. Very approachable and excellent people skills! Thank you.
Hi, are you interested in holding a meeting or an event at central hall westminster london sw1. Hi, I'm Maya, I am an expert venue finder and provide a FREE venue finding service. Save hours by letting me do all of your venue searching for you. Please call me on 0845 351 9917 or alternatively leave your details below and I will happily contact you. Should you know your requirements and wish to make detailed enquiry please click here.


Andre den Exter teaches several courses on Dutch and International health law topics. His main research interests focus on human rights and health care access, pharmaceutical law, health insurance law, European health law, and international treaty law and health care.
Den Exter is a founding member of the Erasmus Observatory on Health Law and coordinates the Observatory's annual Summer school on Health Law & Ethics, Erasmus University Rotterdam. On behalf of the Observatory, and sponsored by the KNAW and ERACOL Eramus Mundus, he organized similar events in Indonesia (Universities of Bandung and Surabaya) (2009 -2011) and Panama (University de Panama)(2011).


A friend asked me the other day if I would be including a story I had written in the anthology of Dorset shorts we are planning to put together as part of the Dorset Writers' Prize. The answer is "No." The competition is not a self-publishing or vanity publishing exercise for me.
With Little Red Writers we want to find and showcase a selection of the best unpublished (so far) stories by writers with a local connection, and I have no wish to diminish the selection process by putting forward something I might have written myself.
Every story submitted will be read and assessed by at least two judges / readers independently. We hope that having readers of differing tastes will help us choose stories of different styles and appeal as we select our first long list of candidates for inclusion in the final book.
As the for the blog - I hope the stories we cite here will highlight some aspects of short story writing from which we might all learn something. They might also give some indication of the varied style of stories I - with all my subjectivities - would love to have sent in.
Ha'penny, by South African anti-apartheid campaigner Alan Paton (best known for his novel Cry, The Beloved Country) seems a simple tale. He has not filled it with poetic description or allegory. It simply tells a tale of a young black boy (Ha'penny) confined to a reformatory in South Africa, as told by the principal in charge.
It is perhaps because of the simplicity of Paton's telling, his control, that his use of the word "prodigal", twice in the final few paragraphs, seems so striking, and reveals some of the unspoken depths of emotion within the story. It also shows that although Paton's words are simple, they are well chosen.
Ha'penny: a small brown coin of little value.
Prodigal: recklessly wasteful or extravagant. Lavish.
PS... I was also pleased (and surprised) to realise while reading Ha'penny that Guy Fawkes Night is celebrated in South Africa. It had never struck me before that it would be - but it gives today's post an added relevance.


Writer Brian Buccellato (FOSTER, DETECTIVE COMICS) returns with another nail biting thriller! Imagine you learn that your dad was essentially Charles Manson and you have the central idea of SONS OF THE DEVIL! We'll be premiering the new IMAGE title at The Comic Bug Manhattan Beach and Brian will be on hand signing copies!


William Tell (German: Wilhelm Tell; French: Guillaume Tell; Italian: Guglielmo Tell) is a legendary hero of disputed historical authenticity who is said to have lived in the alpine Canton of Uri in Switzerland in the early 14th century.
I don't think he's very lucky when he gets shot.
Here's a funny thing about how blizz made this npc. they put an apple object in midspace and a gnome sitting under it. so as the gnome moves the apple stays still. completely messing with laws of physics, matter, and time. i mean by this logic why cant we just fly like super heroes or carry around massive weapons killing people as we please while shooting lazer beams out of our.... o wait... we can do all that stuff.


Posted on September 12, 2016 at 10:58 am.
To play this game you will need a trunk and a peanut.
Place cup upside down on the table. Tape one end of the strip of paper close to the cup's bottom.
Tape the other end of the strip directly across from it creating a loop.
On both ends of each paper tube, punch holes about ½ inch from the openings.
Check the hook from the plastic coat hanger for sharp edges. File down any sharp edges.
Take about 6 inches of string, lay it on the table, place the cut end of the hook in the middle of the string, and tie a knot.
Secure the string using duct tape.
Attach the hook to one of the sections of paper tubing.
Take one end of the string that the hook is on and lace it through one of the holes in one section of paper tube. Tie a knot.
Take the other end of string and lace it through the opposite hole of that section of paper tube, and tie a knot.
NOTE: When held by up by the tube, the hook should hang down from the midpoint of the tube's opening.
Next, connect the tube with the hook to another section using a 2- to 3-inch piece of string. Thread one end of the string through a hole in one section, the other end of the string through a hole in the other section. Tie. Repeat on the other side of the tube.
Continue connecting tubes until there are three or four tubes tied together in a strand with a hook dangling at one end.
For the last two holes of the LAST tube, you will add enough length – about 1½ feet of string to reach behind your head and be able to tie a bow. Take one 1½ foot length of string, thread one end through one of the punched holes, and tie a knot. Take another 1½ foot length of string and do the same with the other hole.
EACH PERSON MUST HAVE HER/HIS OWN TRUNK. Do NOT share trunks. To don the trunk, position the opening of the last tube over the nose, pull the string up over the ears and behind the head where you will tie it with a bow.
Identify the start and a designated area storage area or finish line.
Place the peanut at the starting point.
Using ONLY the trunk pick up the peanut, carry it to the designated storage area, set it down, and remove the hook – NO hands! NOTE: This is a hands free game. Hands may not guide the trunk.
Return to the starting point. THEN go back to the storage area, pick up the peanut, and return home.
Posted on July 31, 2016 at 3:49 pm.
Using the chalk, draw a 3-foot by 3-foot square on the sidewalk or paved driveway.
Create a 3 x 3 grid by drawing lines going down and lines going across at the one-foot and two-foot marks.
The object of the game is to get the highest score possible.
Place the lid/cap on the ground outside of the grid.
Using your thumb and a finger flick the lid.
One attempt per lid/cap. There are no 'do overs' if the lid rolls or lands outside of the grid.
What if the lid lands on a line? No points.
Note: Feel free to change any of the rules. After all, it is your game.
Create a grid with more squares.
Create a grid with smaller dimensions.
Add a rule that the lids/caps MUST land in a straight line (horizontal, vertical, and/or diagonal).
Want to play the game indoors?
Use painters' tape to create the grid on the floor.
Secure a double thick piece of waxed paper at the end of a toilet paper tube with the rubber band.
In the disposable large plastic cup mix ½ cup plaster of Paris and ¼ cup corn starch. Slowly add ¼ to ½ cup water while stirring with the craft stick. Add tempera paint until the desired color is achieved.
Spoon mixture into prepared toilet paper tube. Set the tube upright (waxed paper side down) in an open container while the mixture cures or hardens. Once it is dry peel off the paper tube. It is now ready to use!
IMPORTANT NOT: Do NOT rinse cup in the sink!! Dispose of the cup with the remaining mixture in it. This stuff will clog sink!
Posted on May 27, 2016 at 9:53 am.
Fold string in half. With strings together tie a couple knots at the fold.
Cut one string so that it is 2½ inches shorter than the other string.
To create the balls at the end of the strings, use two 12-inch x 12-inch pieces of aluminum foil.
Tape the end of one string to the center of a foil square. Scrunch the foil to make a tight ball.
Note: Add another layer of aluminum foil if you feel more weight is needed.
Tie a plastic cap, the kind found on squeezable applesauce (see photo), on the end of each string and then cover the cap with aluminum foil.
Note: A wooden bead would also work.
The object of the game is to swing the balls in continuous vertical circles going in opposite directions.
Stand with your dominant arm extending out in front of you with the back of the hand facing outside, as if you were going to shake hands with someone.
Make a fist with your thumb on top of the fist and not crossing over your fingers.
Hold the ball on the shorter string in your non-dominant hand.
Start moving your dominant hand in a rhythmical up and down motion to get the ball on the longer string going in a vertical circle. Note: You may need to a couple of circular motions by the wrist to get the ball going.
If you are holding the toy with the right hand, start the long string ball going in a counter clockwise path.
If you are holding the toy with the left hand, start the long string ball going in a clockwise path.
While keeping a gentle up and down motion going with your dominant hand and wrist, toss the ball on the short string in the opposite direction that the other ball is going.
If you are holding the toy with your right hand, the second ball will go in a clockwise direction.
If you are holding the toy with your left hand, the second ball will go in a counter-clockwise direction.
A steady up and down motion by the hand and wrist will keep the ball circling in opposite directions. Trial and error will let you know the best tempo for keeping the balls in motion.
Posted on April 28, 2016 at 3:51 pm.
According to Games of the World (1977) Nine Men's Morris is one of the oldest board games in the world dating back to 1400 BC. Morris game boards have been found at the temple of Kurna in Egypt and at excavations of the first city of Troy.
NOTE: The game can also be played by using a stick to create the game board in the sand or dirt; pebbles/shells/pinecones can be used for game pieces.
Draw the following game board design onto the paperboard.
– Morris games involve straight lines.
– There are two parts to game play: (1) taking turns placing the game pieces onto the board and then, once all the pieces are on the board, (2) sliding a piece to an adjacent vacant point on the line.
— The 'jumping over' of game pieces is not permitted.
— A player may only move one piece during his/her turn.
In both the placement and then movement of the game pieces, the object is get to three of your game pieces in a row (vertical or horizontal); this is called a 'mill'. Every time a player forms a mill, s/he removes a game piece of the other player. Note: Unless there are no other pieces to take, a game piece may NOT be removed from a mill. Once a piece is removed it may not be returned to the game. A mill may be opened by moving a piece and closed by returning it to its former location, thus creating a new mill.
Posted on February 29, 2016 at 10:46 am.
Fun Fact for February: The bottle cap was patented on February 2, 1892.
Decide ahead of time if there are rules for stacking. For example, "all the caps must have the flat side down".
Whoever has the highest tower of caps without falling wins!
Posted on January 14, 2016 at 10:34 am.
Go back to basics and create your own jigsaw puzzle.
IF using calendar pages, greeting cards, photos, or drawing, glue onto paperboard material. Allow to dry.
Note: IF using the design on the packing material you do NOT need to glue onto another piece of paperboard.
Cut around the images and lettering to create the puzzle pieces.
Posted on December 16, 2015 at 9:50 pm.
Put the two craft sticks together like a sandwich. Wrap the strips of paper completely around each end of the "stick sandwich." Secure the ends of the paper with a piece of tape making sure the tape does not adhere to the sticks.
• Slide out one of the sticks, keeping the paper in place on the other one. Carefully set the stick with the paper on the table.
• On the empty stick, stretch the wide rubber band lengthwise from end to end. Carefully place the stick with the rubber band and set it on top of the stick with the paper. Do not put the rubber band stick inside the paper.
• Wrap a small rubber band around both sticks at each end.
Blow air through the small space between the sticks. Experiment with the amount of airflow to change pitches. Also try pinching the ends while blowing.
Posted on November 24, 2015 at 6:16 am.
Don't let the cold and damp weather of November put a damper on your golf game. Bring the game inside using what you can find around the house.
This activity works best in a room with lots of floor space.
Using duct tape, attach the sponge to the long paper tube to serve as the golf club.
Tapping the ball up and down a ramp made from pieces of cardboard and a stack of books.
Use the various boxes, foam egg cartons, and/or paper tubes to create a barrier or bumper for the golf course.
For the holes, lay the plastic containers, paper, and/or canisters on their sides.
Identify each hold with a flag.
Make a flag by taping a triangular piece of paper onto the flat/smooth end of a bamboo skewer.
Insert the pointed end through exterior center point of the yogurt cup's bottom.
Posted on October 13, 2015 at 3:42 pm.
* This game was named by participants of Big Brothers Big Sisters of Southern Kane and Kendall Counties in Illinois.
Divide and mark the plain side of the panel into 12 squares. Number the squares one through twelve.
The object of the game is to get a pebble in each of the twelve boxes.
Before you begin the game, think about the following situations and establish a rule.
Can't place a pebble: There will be times when a pebble cannot be placed. Does that mean the game is over? Or is this similar to baseball where each player gets three strikes? If there are two or more players, should this result in that person losing the game?
Doubles: What happens when a double is rolled? Does that person get an extra roll? Or does it mean the player loses a turn?
Cover two numbers – one for each die.
As a group where each person takes a turn rolling the dice. If a play cannot be made that person is out.
Posted on October 13, 2015 at 3:37 pm.
In honor of National Hispanic Heritage Month, our Playing from Scratch game this month is Las Chivas, a variation of the game matatenas or as commonly known in the United States, jacks.
Note: The number of pebbles is determined by the size of the player's hand.
Place the bowl on the ground.
Designate a 'tossing line' about 6-to-8 feet from the bowl.
While holding all the pebbles in one hand, Player 1 stands at the 'tossing line' and gently tosses all the pebbles simultaneously toward and hopefully into the bowl. S/he then walks up and removes ONLY the pebbles that are in the bowl. These are then placed on the back of her/his dominate hand. Once these are balanced on the back of the hand the pebbles are flipped into the air and caught in the palm of the same hand. Count the number of pebbles caught. It is now the next player's turn.
The person who with the highest pebble count is the winner.
Quezada, F. (August 30, 2015) Email interview.


The Rolling Stones arrived into Dublin on Sunday evening in timely fashion for their concert on Thursday at Croke Park. Barely had their private jet touched the ground at Dublin Airport before singer Mick Jagger was on his way to catch the end of the cricket match between Ireland and Pakistan at Malahide.
Bands do not normally allow themselves four days' preparation for a concert, but this is the first date on the second leg of the Stones' No Filter tour. The members of the band, Jagger, Keith Richards, Charlie Watt and Ronnie Wood need the time in Ireland to rehearse a new set list.
However, the look and feel of the show will be the same as the previous leg of the tour, which ended in Paris last Octover. "It's expensive to change it," said long-time tour manager Dale Skjerseth.
Fans attending the Croke Park concert have been warned by Aiken Promotions events controller Jim Clarke to travel light and to bring "just yourselves and a sense of excitement for the act".
The Rolling Stones have a "consistency in how they handle their audience" when it comes to security, he explained. The band has insisted no bag bigger than an A5 sheet paper will be allowed into the arena.
It will be a busy week of concerts in Dublin. The Rolling Stones gig takes place between Ed Sheeran's three concerts in the Phoenix Park tomorrow, Friday and Saturday nights. All three of Sheeran's concerts are sold out.
Why should the taxpayer bail out RTÉ over orchestra mess?


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Listing provided courtesy of Aspire Realty Inc.


1st day of Week 19 and the 29th day of the cycle of Tiferet (Balance/Beauty), Chesed shebeHod shebeTiferet, Kindness within Glory/Acknowledgement within Balance/Beauty.
Redeem me from the oppression of man, and I shall keep Your precepts.
Portion is about redemption from Egypt and the precepts of Passover. The main idea is that there should not be leavened, in order to remember our poor state in Egypt.
7. This poor man called, and the Lord heard; He delivered him from all his tribulations.
That is why we heard only these two commandments, "I am…," and "You shall not have…," directly from G‑d, as our Sages have said, for they are the sum total of the whole Torah.
The chapter goes on to explain how "all is as naught beside Him, as if absolutely nonexistent."
Rotzeach uShmirat Nefesh, Chapter Seven, is about those guilty of manslaughter who are exiled to a City of Refuge. They are saved from those that would avenge the blood of the person killed, and are granted everything necessary for them to live and serve G-d. A Torah scholar who is exiled, must be exiled with his teacher!
One must recite words of Torah copiously ("מרבה זיין אין זאגען אותיות התורה" lit. to increase in saying letters of Torah), saying Tehillim or verbally reviewing Mishna whenever and wherever possible, in order to bolster the existence of Creation, to be saved from chibut hakever and kaf hakela, and to merit all the highest revelations.
Bava Basra 10 is all about giving charity to the poor.


We are looking for information that will help us locate Spencer Hughes.
The 30-year-old has been missing since 1610 on 09/04, when he was last seen at Skegness train station.
Spencer is described as tall, thin build, with brown hair. It is believed he could be in the Skegness, Nottingham or Mansfield areas.
He was last seen wearing a black North face coat, blue jeans, and black trainers.


It probably would have to have been some of the more talented low level fellers like Romo or Bucardo or even Pucetas (he's got the numbers to give Billy boy a little chub). IMO, those are the guys the guys shouldn't consider trading. By the time the team is good, they'll be need on the big club.
Swisher being traded is just Teh Curse of Joey K. My buddy is a big time Athletics Supporter. The year that the A's acquired Johnny Damon he sunk $200 into an authentic road grey Damon jersey. Shorty after, Damon was Boston-bound. He thought he learned from his mistake and decided to invest in a nice Home White with a young player who was sure to be in with the club at least through his arbitration years. Now that $200 Nick Swisher Jersey can hang proudly in his closet by the Damon Uni.
Well, I think that lesson has as much to do with spending $200 on an article of clothing as investing in a particular player.
Some KNBR caller was whining about buying his son an $80 Swisher jersey and how Beane owes him a refund.
To him and anyone else that develops man-crushes on players: it's not worth it. Love 'em while they're on your team but don't go boiling their bunnies when they go.
So are we going to sign off each post with a relevent player or coach, because I just don't care to put the effort in.
That's not a very good attitude. Just "Do you job" like I did.
Some people seem to have missed the point. This started over in the "Sabean do you WANT us to hate you" thread when someone posted : "Fresno blasts may not translate to the bigs. " That was followed by fake quotes from people who hit well in Fresno, but never managed to hit well when playing for the big club. (Torcato, Linden, Ransom, Ellison, etc).
Then some people completely misunderstood and started posting random quotes.
"Events are not as random as they appear to be."
"Sh-it, are you going to quote me?"
Ortmeier, Fred Lewis and R Davis would have to pass waivers. (and Davis *might* be able to refuse an outright assignment to the minors).
Schierholtz can be sent to Fresno for one more year without having to worry about waivers.
I believe that both Erick Threets and Merkin Valdez are out of options and would have to pass waivers as well.
Yeah, but neither of those guys should start the year in SF. They should simply be dropped from the 40-man (as space is needed). I only addressed position players - not any of the pitchers.


Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).
Wagner, M., Koester, H., Deffge, C., Weinert, S., Lauf, J., Francke, A., Lee, J., Braun- Dullaeus, R. C., Herold, J. Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes. J. Vis. Exp. (94), e52347, doi:10.3791/52347 (2014).
As a subtype of leukocytes and progenitors of macrophages, monocytes are involved in many important processes of organisms and are often the subject of various fields in biomedical science. The method described below is a simple and effective way to isolate murine monocytes from heterogeneous bone marrow.
Bone marrow from the femur and tibia of Balb/c mice is harvested by flushing with phosphate buffered saline (PBS). Cell suspension is supplemented with macrophage-colony stimulating factor (M-CSF) and cultured on ultra-low attachment surfaces to avoid adhesion-triggered differentiation of monocytes. The properties and differentiation of monocytes are characterized at various intervals. Fluorescence activated cell sorting (FACS), with markers like CD11b, CD115, and F4/80, is used for phenotyping. At the end of cultivation, the suspension consists of 45%± 12% monocytes. By removing adhesive macrophages, the purity can be raised up to 86%± 6%. After the isolation, monocytes can be utilized in various ways, and one of the most effective and common methods for in vivo delivery is intravenous tail vein injection.
This technique of isolation and application is important for mouse model studies, especially in the fields of inflammation or immunology. Monocytes can also be used therapeutically in mouse disease models.
The isolation of monocytes is important and critical for many in vitro and in vivo studies. These cells are targets for diseases such as peripheral arterial disease, coronary heart disease, or other ischemic diseases, since collateral vessel growth is strongly driven by local inflammation. Inflammatory responses include endothelial activation and local recruitment of leukocytes, mainly monocytes, which then mature to macrophages and create a highly arteriogenic environment by secreting multiple growth factors to induce the remodeling of an arteriole into a functional collateral artery1-3. Monocytes also mature to dendritic cells, which are frequently used for immunological studies4,5 and cancer research6,7.
Problematic in the approach for monocyte isolation from peripheral blood8 is the high number of donor animals needed to produce a sufficient amount of monocytes for most analyses. Former protocols describe methods such as density gradient centrifugation and cell depletion via MACS9 when isolating monocytes; however, these techniques can alter the characteristics and functionality of monocytes which can lead to difficulties in interpretation10,11. Moreover, these methods are difficult and can reduce experimental reproducibility.
Our aim with this protocol is to provide a simple and cost effective method to generate large amounts of bone marrow-derived monocytes. Due to the high cell yield of 11 x 106 ± 3 x 106 cells obtained by this protocol, we can substantially reduce the number of mice required during the isolation of bone marrow-derived monocytes. The procedure can be completed within a minimal amount of time, and without using expensive and complicated techniques as referenced above. Here, we extract monocytes from native bone marrow suspension of donor mice, cultivate the suspension on ultralow attachment plates, and supplement the solution with 20 ng/ml M-CFS. On day 5 of incubation, cells are harvested and characterized to confirm functional and phenotypic properties.
For experiments in the field of arteriogenesis, intravenous transplantation of these bone marrow-derived monocytes into mice is an effective method of systemic drug delivery, which can be combined with femoral artery ligation in common peripheral arterial disease models.
This study was performed with permission of the State of Saxony and Saxony-Anhalt, Regierungspraesidium Dresden/Halle, according to Section 8 of the German Law for animal protection (24D-9168.11-1/2008-24).
Anesthetize the mouse using a 5% isoflurane concentration by vaporizing isoflurane in a closed bin. After the mouse has stopped moving for 3 sec, perform the cervical dislocation.
Disinfect the hind limbs with ethanol (96%). Remove skin and muscles and separate the bones with a sterile scalpel.
Start with an inguinal skin incision, and extend the incision toward the medial malleolus and perform a circular dissection of the skin around the lower calf. Remove the skin of the legs completely by peeling it proximally.
Detach the quadriceps muscle from the femur with a sharp scalpel, remove both the anterior peroneus and the gastrocnemius muscle groups and disarticulate the leg at the hip joint by locating and dissecting the ligaments.
Start anteriorly and proceed around the joint by carefully rotating the leg and transecting the ligaments, thereby disarticulating the joint.
Wash femur and tibia with 96% ethanol for a minimum of 90 sec to guarantee aseptic cell preparation. Continue the washing process with sterile PBS to rinse off remaining ethanol.
Note: All subsequent steps must be sterile to avoid contamination.
Cut the proximal and distal end of each bone with a pair of fine scissors to gain access to the femoral and tibial shafts. Be careful with this step, since these bones break very easily.
Flush the bones with warm medium (M199 + 10% fetal calf serum (FCS), + 1% Penicillin/Streptomycin). Use a sterile 28-G needle, a 1 ml syringe, and between 10-15 ml medium per bone for rinsing.
Hold the bone with a fine forceps and rinse one by one until it turns semi-translucent. Carefully apply pressure to the end of the syringe plunger in order to minimize cell stress.
Filter the bone marrow through a 70 µm nylon web and gather the filtrate. To extract the maximum quantity of bone marrow, rinse repeatedly from the distal and proximal ends.
Rinse the sieve with PBS and gather the filtrate. Carefully dislodge debris and cellular conglomerates by gentle stirring and pipetting.
Centrifuge the cell suspension at 250 x g for 10 min at RT. Discard the supernatant and resuspend the cells with approximately 25 ml of medium. Repeat once.
Seed the cells on 6-well ultra-low-attachment surface plates to prevent permanent adhesion to the bottom of the plate. Use a concentration of 106 cells per ml with up to 6 ml per well.
Supplement the suspension with 20 ng/ml M-CSF to promote cell differentiation.
Culture the cells for 5 days at 37 °C and 5% carbon dioxide and observe daily.
Note: These steps should be performed on ice. Proceed with either 1.4.1 or 1.4.2 accordingly.
At this point, harvest the entire culture, including differentiated macrophages that will adhere to the culture-plates, even if they are ultra-low-attachment surface plates.
Harvest the entire culture by gentle pipetting and detaching with a solution of 4 °C PBS, 0.5% bovine serum albumin (BSA) and 2 mM EDTA. Repeat until the plates are clear of remaining adherent cells - confirm under a microscope if unsure.
Alternatively, discard adherent macrophages by harvesting the supernatant with the cells in suspension, which predominantly contain monocytes.
Harvest the non-adherent cells with a solution of EDTA-free PBS and 0.5% BSA. Do not apply too much force in order to avoid dislodging mildly adherent macrophages.
Note: It is possible to deplete the cell suspension of CD117+ stem- and progenitor cells by the use of MACS. Use manufacturer protocols for this procedure.
Harvest the cells according to either steps 1.4.1 or 1.4.2.
Centrifuge the cells at 250 x g for 10 min at 4 °C and repeat this step again.
Resuspend at least 250,000 cells in 300 µl of FACS buffer per probe.
Transfer cell solution onto a 96-well-plate (30 µl per well).
Remove the supernatant and add 25 µl of antibody to each well (use manufacturer dilution).
Stain the cells with antibodies (following manufacturer protocols for cell phenotyping after steps 1.5.1-1.5.6. Commonly used markers for monocyte / macrophage identification include CD11b, F4/80 (Figure 3), CD115 (Figure 2), and Gr-1.
Note: For a further description of cell solutions, the use of markers like CD4, CD8a, CD11c, CD25, CD45, CD45R, CD62L, CD80, CD86, CD145, CD117, MHCⅡ, Ly6C, Ly6G, and CD192 is recommended. Cellular characteristics were previously described12.
Incubate the probes for 20 min at 4 °C.
Centrifuge the probes at 250 x g for 5 min at 4 °C and resuspend with 200 µl of FACS buffer. Repeat this step twice.
Transfer the probes into FACS tubes and perform the FACS analysis12.
Perform FACS analysis12 on day 0, 3 and 5 to analyze cell differentiation.
Warm the animals on a heating plate at 37 °C for about 10 min. Observe the animals while warming to recognize signs of overheating.
Resuspend the monocytes, isolated in steps 1.1- 1.4, in 150 µl of NaCl, and load the cells into a 1 ml insulin syringe with a 30G needle. Lightly vortex the solution before loading the syringe to ensure all monocytes are injected. Always handle cells on ice to avoid heat-mediated attachment and activation of monocytes.
Avoid disturbing other mice in the cage when choosing a mouse for intravenous injection.
Note: Alternatively, use general anesthesia to guarantee stress free handling of the animals.
Ensure that the mouse has space for breathing before closing the restrainer.
Disinfect the injection site with disinfection agent. Spray on the tail of the mouse and let stand for at least 1 min.
Stop venous blood flow of the tail by applying gentle pressure to the lateral tail side above the injection site, for better visibility of the veins.
Inject at a 45 degree angle. Inject slowly and no more than 5 µl per g to avoid harming animals.
If there is a blister, which is a sign of a failed injection, discontinue immediately and repeat the injection more proximally.
Stop the bleeding at the injection side by applying gentle pressure for about 1 min.
At the end of the procedure, open the retractor and place the mouse in its cage.
Observe the mouse for 20 min to ensure that the animal was not harmed by the injection and sternal recumbency is maintained. Extend the time of observation until the mouse regains sufficient consciousness. Return the mouse to the company of other animals only after it has fully recovered.
The cell solution extracted from the murine bone marrow consists of various cell types. The major cell types are lymphocytes, granulocytes and monocytes. Cell types can be estimated by size and granularity, which is shown in Figure 1 for both native suspensions and cells harvested after 5 days of differentiation. Note the shifting cellular composition during cultivation. However, accurate classification of populations must rely on distinctive expression of cellular markers.
The most important marker used to identify cells of the MPS-System is CD115, which functions as the M-CSF receptor and is specifically expressed on monocyte progenitors, monocytes and macrophages. Therefore, it might not be used to identify distinctive MPS-subsets, but can reliably estimate the absolute amount of cells driven into the monocyte/macrophage differentiation. See Figure 2 for a timeline of CD115 expression during differentiation.
The maturity marker F4/80 is useful to differentiate juvenile monocytes, mature monocytes, and macrophages. Macrophages show strong expression of F4/80 in comparison to juvenile monocyte progenitors, which are negative for the marker. Mature monocytes show intermediate expression of F4/80. A combination of CD115 and F4/80 markers therefore represents a feasible method for quantifying and monitoring cell differentiation (see Figure 3).
The combination of CD115 and F4/80 is sufficient for routine culture observation and quality control prior to application of cells. A more detailed examination of the bone-marrow-derived monocytes at day 5 of cultivation confirms their structural and functional properties in concordance to those of peripheral blood monocytes 12.
It is important to fix the mouse carefully in the restrainer, avoiding tension on the tail. Restrict freedom of movement as much as possible without inhibiting breathing to facilitate the injection.
Figure 1. Left panel: Composition of cell types at day 1 in culture. Cell types include lymphocytes, monocytes and granulocytes. Right panel: Note the shifting cellular composition during cultivation. Cell population includes monocytes and macrophages. Please click here to view a larger version of this figure.
Figure 2. Timeline of CD115 expression during differentiation. Note that >95% of all cells are CD115 positive after 5 days of differentiation, indicating that the vast majority of cells are either monocytes or macrophages. Classification can be based on cell size and granularity, but specific cellular markers are more reliable. Please click here to view a larger version of this figure.
Figure 3. Increasing expression of monocyte/macrophage maturity marker F4/80. Day 5: Note the appearance of a population with intermediate F4/80 expression, which is consistent with monocyte phenotype. Day 7: Note the right-shift of F4/80 expression, consistent with macrophage maturation. Please click here to view a larger version of this figure.
Figure 4. Mouse fixed in restrainer for tail vein injection. After carefully restraining the mouse, rotate the tail 90 degrees until the veins are visible on the dorsal side. Please click here to view a larger version of this figure.
Figure 5. Schematic cross section of the mouse tail. The picture illustrates the location of vessels within the mouse tail. The arteries (red) are located on the ventral and dorsal side, and the veins are on the lateral side of the tail. Please click here to view a larger version of this figure.
We describe a simple and cost-effective method to isolate large amounts of murine monocytes from bone marrow. In comparison to other protocols using peripheral blood, which obtain monocyte yields5 of 1.4 x106, we are able to obtain higher yields of 11 x 106 ± 3 x 106 monocytes from a single donor mouse.
When considering challenges with this method, it is important to mention the potential for contamination when working under non-sterile conditions. If rinsing and the following washing steps are not followed properly, contamination by bacteria and fungi is likely. Additionally, bone marrow consists of heterogeneous cell suspensions, including granulocytes, lymphocytes and erythroid cells. Normally these cells vanish between day 2 and 3 of cultivation due to the lack of growth factors. After growth factor driven differentiation, macrophages are the major source of contamination. To keep the number of macrophages low and to minimize the differentiation of monocytes to macrophages, the use of ultra-low attachment plates and the washing steps mentioned above are critical.
It is difficult to identify a marker that is exclusively monocyte-specific. Interference between markers necessitates the use of more than one marker, and there is variability in the granularity and size of cells in analyzing cell suspensions. The markers and descriptions shown in Figures 1-3 are good alternatives for representative cell types in culture. It can be difficult to distinguish the various cell types, especially after day 5. Therefore it is critical to use other markers like CD115 and F4/80.
Considering the expansion of bone marrow cells under adhesion-free conditions, macrophage differentiation can be prolonged and undifferentiated monocytes can accumulate. Macrophage differentiation indicates contamination; however, due to their adhesive nature even on ultra-low-attachment cultivation surfaces, they can be easily discarded while only harvesting non-adhesive cells from culture. In previous experiments1,3, contamination by macrophages has not led to serious clinical side effects. Histological workup of organs and muscles of mice after cell transplantation showed that injected macrophages assimilated in organs like spleen, liver or lung. The assimilation of macrophages in these organs demonstrated no observable clinical effects in mice. The molecular mechanism triggered by these macrophages can be interesting questions for further investigations.
Monocytes as well as macrophages affect arteriogenesis in peripheral arterial disease, especially when applied systemically. Clinical side effects such as embolism or massive systemic inflammation could not be observed even after injection of more than 2.5 million monocytes, but these cells can trigger inflammation and potentially cause severe systemic effects. In human trials, these effects are likely to have clinical significance. The triggering of arteriogenesis can affect tumor growth or diabetic retinopathy, and such possible systemic effects of injected cell solutions should be explored in future studies.
Previous publications9 describe differences between the characteristics of peripheral blood- and bone marrow-derived monocytes, which explain why the properties of monocytes from bone marrow can differ from those isolated from peripheral blood cells.
The method of isolation is of clinical interest as well. Drawing blood instead of extracting human bone marrow is more practical in the context of clinical medicine. It is important to look after the welfare of the animals and to apply analgesia if needed. For intravenous injections, it is critical to practice handling both the animal and the syringe at the same time. If a single animal is undergoing multiple injections, the quality of the veins and the skin of the tail suffer.
Despite these disadvantages, this method is very useful to study the behavior and characteristics of monocytes. Experiments in the field of atherosclerosis, immunology or inflammation can benefit from this method. Only 30 min are required from the extraction of bone marrow to the seeding of cells on 6-well ultra-low-attachment surface plates. Ethical concerns are minimized, because the high yield of this protocol minimizes the number of animals required as cell donors. This new method will be helpful for many studies involving monocytes.
We have been focused on therapeutic augmentation of collateral vessel growth via transplantation of monocytes. The multifactorial nature of this process may explain why single factor approaches for augmentation of arteriogenesis have generated mixed results13,14 . This has led to the investigation of cell-based therapies. Autologous bone marrow-derived stem and progenitor cells have been identified as potential cells for transplantation, but only moderate clinical benefits have been reported15. Besides research on dosage, isolation methods, and administration routes, further research is still needed to determine the optimal cell type. A disadvantage of autologous cell transplantation could be their inability to activate the innate and/or adaptive immune response, and both are critical for arteriogenesis. Increasing local inflammation may represent the best stimulus for arteriogenesis16.
Intravenous injection of monocytes is a common method for cell transplantation within mouse models if systemic drug delivery is desired. Because of systemic effects, side effects can occur as well. Ensure that the vein is targeted for injection. It is common to confuse arteries and veins, especially when injecting heavily pigmented mice. Arterial injection can cause emboli, which lead to systemic issues in circulation.
This work was supported by the DFG (Deutsche Forschungsgemeinschaft, German Research Foundation) SFB 854 (Sonderforschungsbereich, collaborative research center).
Thanks to Hans-Holger Gärtner, Audiovisuelles Medienzentrum, Otto-von-Guericke University Magdeburg, Magdeburg, Germany, for technical support.
Herold, J., et al. Transplantation of monocytes: a novel strategy for in vivo augmentation of collateral vessel growth. Hum Gene Ther. 15, (1), 1-12 (2004).
Herold, J., Tillmanns, H., Xing, Z., Strasser, R. H., Braun-Dullaeus, R. C. Isolation and transduction of monocytes: promising vehicles for therapeutic arteriogenesis. Langenbecks Arch Surg. 391, (2), 72-82 (2006).
Francke, A., Weinert, S., Strasser, R. H., Braun-Dullaeus, R. C., Herold, J. Transplantation of bone marrow derived monocytes: a novel approach for augmentation of arteriogenesis in a murine model of femoral artery ligation. American Journal Of Translational Research. 5, (2), 155-169 (2013).
Leon, B., et al. Dendritic cell differentiation potential of mouse monocytes: monocytes represent immediate precursors of CD8- and CD8+ splenic dendritic cells. Blood. 103, (7), 2668-2676 (2004).
Timmerman, J. M., Levy, R. Dendritic cell vaccines for cancer immunotherapy. Annu Rev Med. 50, 507-529 (1999).
Salem, M. L. The use of dendritic cells for Peptide-based vaccination in cancer immunotherapy. Methods Mol Biol. 1139, 479-503 (2014).
Timmerman, J. M., et al. Idiotype-pulsed dendritic cell vaccination for B-cell lymphoma: clinical and immune responses in 35 patients. Blood. 99, (5), 1517-1526 (2002).
Berthold, F. Isolation of human monocytes by Ficoll density gradient centrifugation. Blut. 43, (6), 367-371 (1981).
Houthuys, E., Movahedi, K., De Baetselier, P., Van Ginderachter, J. A., Brouckaert, P. A method for the isolation and purification of mouse peripheral blood monocytes. Journal Of Immunological Methods. 359, (1-2), 1-10 (2010).
Seeger, F. H., Tonn, T., Krzossok, N., Zeiher, A. M., Dimmeler, S. Cell isolation procedures matter: a comparison of different isolation protocols of bone marrow mononuclear cells used for cell therapy in patients with acute myocardial infarction. European Heart Journal. 28, (6), 766-772 (2007).
Auffray, C., et al. CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation. The Journal Of Experimental Medicine. 206, (3), 595-606 (2009).
Francke, A., Herold, J., Weinert, S., Strasser, R. H., Braun-Dullaeus, R. C. Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties. J. Histochem. Cytochem. 59, (9), 813-825 (2011).
Sneider, E. B., Nowicki, P. T., Messina, L. M. Regenerative medicine in the treatment of peripheral arterial disease. Journal Of Cellular Biochemistry. 108, (4), 753-761 (2009).
Van Royen, N., Piek, J. J., Schaper, W., Fulton, W. F. A critical review of clinical arteriogenesis research. J Am Coll Cardiol. 55, (1), 17-25 (2009).
Lawall, H., Bramlage, P., Amann, B. Treatment of peripheral arterial disease using stem and progenitor cell therapy. J Vasc Surg. 53, (2), 445-453 (2011).
Herold, J., et al. Tetanus toxoid-pulsed monocyte vaccination for augmentation of collateral vessel growth. Journal of the American Heart Association. 3, (2), e000611 (2014).


text:
Q: How to dynamically reconfigure Drupal's jQuery-based autocomplete at runtime? Drupal has a very well-architected, jQuery-based autocomplete.js. Usually, you don't have to bother with it, since it's configuration and execution is handled by the Drupal form API.
Now, I need a way to reconfigure it at runtime (with JavaScript, that is). I have a standard drop down select box with a text field next to it, and depending what option is selected in the select box, I need to call different URLs for autocompletion, and for one of the options, autocompletion should be disabled entirely. Is it possible to reconfigure the existing autocomplete instance, or will I have to somehow destroy and recreate?

A: Have a look at misc/autocomplete.js.
/**
 * Attaches the autocomplete behavior to all required fields
 */
Drupal.behaviors.autocomplete = function (context) {
  var acdb = [];
  $('input.autocomplete:not(.autocomplete-processed)', context).each(function () {
    var uri = this.value;
    if (!acdb[uri]) {
      acdb[uri] = new Drupal.ACDB(uri);
    }
    var input = $('#' + this.id.substr(0, this.id.length - 13))
      .attr('autocomplete', 'OFF')[0];
    $(input.form).submit(Drupal.autocompleteSubmit);
    new Drupal.jsAC(input, acdb[uri]);
    $(this).addClass('autocomplete-processed');
  });
};

The input's value attribute is used to create ACDB, which is a cache of values for that autocomplete path (uri). That is used in the Drupal.jsAC function to bind the element's keydown, keyup and blur events with triggers the autocomplete ajax operation (which caches its values in the ACDB object for that element), opens popups, etc.
/**
 * An AutoComplete object
 */
Drupal.jsAC = function (input, db) {
  var ac = this;
  this.input = input;
  this.db = db;

  $(this.input)
    .keydown(function (event) { return ac.onkeydown(this, event); })
    .keyup(function (event) { ac.onkeyup(this, event); })
    .blur(function () { ac.hidePopup(); ac.db.cancel(); });

};

What you'll need to do is change the input's value and also reattach the behavior. You'll reattach the behavior by removing the '.autocomplete-processed' class on the autocomplete text field input element and then call Drupal.attachBehaviors(thatInputElement).
This may not work. Things can go very badly if you attach the same behavior to the same element over and over again. It may be more sensible to create different autocomplete fields and simply hide and show them based on the value of the select. This would still require calling Drupal.attachBehaviors when you hide and display the widget, but the same behavior would remain attached if the switch happened more than once, and you wouldn't risk attaching the same behavior to the element multiple times.

A: Well, for reference, I've thrown together a hack that works, but if anyone can think of a better solution, I'd be happy to hear it.
Drupal.behaviors.dingCampaignRules = function () {
  $('#campaign-rules')
    .find('.campaign-rule-wrap')
      .each(function (i) {
          var type = $(this).find('select').val();

          $(this).find('.form-text')
            // Remove the current autocomplete bindings.
            .unbind()
            // And remove the autocomplete class
            .removeClass('form-autocomplete')
          .end()
          .find('select:not(.dingcampaignrules-processed)')
            .addClass('dingcampaignrules-processed')
            .change(Drupal.behaviors.dingCampaignRules)
          .end();

          if (type == 'page' || type == 'library' || type == 'taxonomy') {
            $(this).find('input.autocomplete')
              .removeClass('autocomplete-processed')
              .val(Drupal.settings.dingCampaignRules.autocompleteUrl + type)
            .end()
            .find('.form-text')
              .addClass('form-autocomplete');
            Drupal.behaviors.autocomplete(this);
          }
      });
};

This code comes from the ding_campaign module. Feel free to check out the code if you need to do something similar. It's all GPL2.

A: it should be as simple as dinamically change the "value" of the "hidden" autocomplete
input element that comes aside autocomplete form fields. ie.

$('select#myelement').bind('change', function(e) {
  if (/* something */) {
    $('input#myelement-autocomplete').attr('value', '/mycustom/path');
  }
});

A: Working solution for Drupal 5
/*
 *  Błażej Owczarczyk
 *  blazej.owczarczyk@gmail.com
 *
 *  Name: Autocomplete City Taxonomy
 *  Description: Hierarchical city selecting (province select and city autocomplete)
 */

var Act = Act || {};

Act.init = function () {
    $('select.act-province').change(Act.provinceChange);        // select with top taxonomy terms
}

/*
 *  Change event of select element
 */
Act.provinceChange = function () {
    var context = $(this).parent().parent();
    var currentTid = $(this).val();
    Act.rewriteURI(context, currentTid);
    Act.unbind();
    Drupal.autocompleteAutoAttach();
};

/*
 *  Changes the value of hidden autocomplete input
 */
Act.rewriteURI = function (context, newTid) {
    var tempArray;
    tempArray = $('.autocomplete', context).val().split('/');
    tempArray.pop();
    tempArray.push(newTid);
    $('.autocomplete', context).val(tempArray.join('/'));
};

/*
 *  Prevents muliple binding of the same events
 */
Act.unbind = function () {
    $('.form-autocomplete').unbind().parents('form').unbind('submit');
};

$(document).ready(Act.init);


text:
Q: implementing coredata into existing application, create or autowritten sqllite I am integrating core data into my application, I already have a sqllite DB file.  Should I create a new one to make it easier, or should I use the existing one.
I am sorry for the many questions, thank you in advanced!!
Also, how do I create a new one?
I implemented the methods below into appdelegate (no errors), however I don't know what to put in the text fields
What is this?  Is "myCoreData" the name of the core data db created with a .xcdatamodeld ending?  If so, what is momd?
NSURL *modelURL = [[NSBundle mainBundle] URLForResource:@"myCoreData" withExtension:@"momd"];

what is this?
Does this create the database, or should I create it and put the info here? Where is this stored?
NSURL *storeURL = [[self applicationDocumentsDirectoryModified] URLByAppendingPathComponent:@"coreDataDB.sqlite"];

this is the implementation
- (void)saveContext
{
    NSError *error = nil;
    NSManagedObjectContext *managedObjectContext = self.managedObjectContext;
    if (managedObjectContext != nil) {
        if ([managedObjectContext hasChanges] && ![managedObjectContext save:&error]) {
            // Replace this implementation with code to handle the error appropriately.
            // abort() causes the application to generate a crash log and terminate. You should not use this function in a shipping application, although it may be useful during development.
            NSLog(@"Unresolved error %@, %@", error, [error userInfo]);
            abort();
        }
    }
}

#pragma Core Data stack

// Returns the managed object context for the application.
// If the context doesn't already exist, it is created and bound to the persistent store coordinator for the application.
- (NSManagedObjectContext *)managedObjectContext
{
    if (_managedObjectContext != nil) {
        return _managedObjectContext;
    }

    NSPersistentStoreCoordinator *coordinator = [self persistentStoreCoordinator];
    if (coordinator != nil) {
        _managedObjectContext = [[NSManagedObjectContext alloc] init];
        [_managedObjectContext setPersistentStoreCoordinator:coordinator];
    }
    return _managedObjectContext;
}

// Returns the managed object model for the application.
// If the model doesn't already exist, it is created from the application's model.
- (NSManagedObjectModel *)managedObjectModel
{
    if (_managedObjectModel != nil) {
        return _managedObjectModel;
    }
    NSURL *modelURL = [[NSBundle mainBundle] URLForResource:@"myCoreData" withExtension:@"momd"];
    _managedObjectModel = [[NSManagedObjectModel alloc] initWithContentsOfURL:modelURL];
    return _managedObjectModel;
}

// Returns the persistent store coordinator for the application.
// If the coordinator doesn't already exist, it is created and the application's store added to it.
- (NSPersistentStoreCoordinator *)persistentStoreCoordinator
{
    if (_persistentStoreCoordinator != nil) {
        return _persistentStoreCoordinator;
    }

    NSURL *storeURL = [[self applicationDocumentsDirectoryModified] URLByAppendingPathComponent:@"coreDataDB.sqlite"];

    NSError *error = nil;
    _persistentStoreCoordinator = [[NSPersistentStoreCoordinator alloc] initWithManagedObjectModel:[self managedObjectModel]];
    if (![_persistentStoreCoordinator addPersistentStoreWithType:NSSQLiteStoreType configuration:nil URL:storeURL options:nil error:&error]) {

        NSLog(@"Unresolved error %@, %@", error, [error userInfo]);
        abort();
    }

    return _persistentStoreCoordinator;
}

A: Did not use Magical record, Ended up adding the boilerplate code from a new project in xcode. Start a simgle view application, click coredata, go to adddelegate, at the bottom are most the methods you need.
To make it easier, I created a communicator with all the methods that I needed for coredata.


As the name suggests, LinenWeave™ Covers evoke the elegant, natural fibre look and feel of linen. Made from a high content wood pulp and recycled materials, these sturdy A4, 250gsm covers incorporate colour fastening features to ensure your reports stay looking pristine and don't fade over time. With a classic colour palette to match, LinenWeave™ Covers give a top quality, professional finish to presentations and reports.


Telanagana State Integrated Common Entrance Test-(TSICET-2019) of Telangana State will be conducted for the applicants through ONLINE MODE by Kakatiya University, Warangal for admission into first year a) MBA and b) M.C.A. for the academic year 2019-20 in the State of Telangana.
FN-10.00 A.M. to 12.30 P.M.
FN 10.00 A.M. to 12.30 P.M.
The Registration fee should be paid through payment gateway (Credit Card/Debit Card/ Net Banking /TS Online / AP Online).


text:
Who Is Afraid of Hillary Clinton?
At last night's GOP slugfest, what emerged was not a platform but 10 candidates deflecting, fingerpointing, and demonstrating genuine fear of a Dem they've decided is our next president.
Kera Bolonik Oct 28, 2015
On Wednesday nights in the fall, I like to do one thing: tuck into a neat splash of Balvenie single malt Scotch, and turn on my American Horror Story. Yeah, I know the show takes weird, literally incredible and gross twists and turns—I signed on for all of them—but how Ryan Murphy and Brad Falchuk have catapulted us from that beautiful, hell-creepy Art Deco hotel in L.A. to a stage in Boulder, Colorado, I dunno … I am just going to have to take a leap of faith with them on this episode. It seems he's making Kathy Bates's Iris emerge from her suicide attempt to live in a worse purgatory than being the desk clerk at the blood-soaked Hotel Cortez by inhabiting the skin of Arkansas's former governor and racist tweeter Mike Huckabee. And why is Denis O'Hare's Liz Taylor wearing Rand Paul's wig? Hooray! I thought Lily Rabe wasn't going to be in this season's AHS, but lo and behold, there she is, playing Carly Fiorina … wait, that really is Donald Trump.
Oh, crap. I am looking at the clock: My show isn't on for another hour. I'm watching a different horror show, one that might even be too gruesome for Murphy and Falchuk to touch, this one on CNBC, with ten GOP presidential hopefuls scrambling hysterically on a sinking Titanic, wielding deck chairs in search of a dry and safe place to plant them. One of them sneaks away and jumps on a life boat by appearing to pull himself together as the least crazy, least combative, and most coherent—and that guy, is Marco Rubio.
Now let me be clear, he is not the least offensive. There is no such person up there. Donald Trump could be cast as Rubio's anger translator, a la Key and Peele. When CNBC moderator John Harwood asked the Donald if he was running a "comic book version of a presidential campaign," it was really a question that should be put to each candidate. Yes, Trump insists that the U.S. needs to build a wall across the Mexican border (hey, China did it and ours wouldn't have to be so big), and get Mexico to foot the bill. "People say, 'Oh, how are you gonna get Mexico to pay?' A politician can't get them to pay," said Trump, "I can." At least he's honest about being delusional.
But how is that crazier than Rubio's calm assertion that the mainstream media is a "super-PAC" devoted to protecting liberals? Or that the House Select Committee's 11-hour interrogation of Hillary Clinton, led by Trey Gowdy, was anything but a fishing expedition? That it actually exposed Hillary as "a liar"? If it exposed anything, it was that the GOP couldn't figure out what their angle was. As Rolling Stone's Matt Taibbi, who is far from being a Hillary fan, asked, "The overriding implication of the Benghazi hearing seemed to be that Hillary Clinton was so crass, unfeeling, and politically self-involved as to not care if members of her State Department were massacred. Hillary has a lot of flaws, but we're supposed to believe that she doesn't have a problem with dead Americans? Seriously?"
Rubio is a silent killer, like carbon monoxide. And he, not current front-running clowns Trump or the self-described volatile Dr. Ben Carson, is going to be the GOP presidential candidate next November. I hope to God not our president.
Look, I am by no means a policy wonk or political analyst. And perhaps being an outsider in this instance is an advantage and not a flaw for two reasons. For one, three of the ten people on that stage are not politicians—Carson, the brain surgeon who doesn't believe in climate change, aside, lies even as he's having the facts being flung into his face; Carly, who couldn't answer why America should hire a CEO who was fired from the company she tanked; and of course, Donald, whose policies rely on his ego, and who is finally falling in the polls. The other seven are former and present senators and governors who blame the government for stealing money from Americans, while forgetting, it seems, that they are willingly employed by it, and are among the perps. And when backed into a corner, blame the mainstream media. And warn that a world with Hillary is far more terrifying than climate change (which humans have nothing to do with, by the way). At least they were all on the same page there. The rest of the time, they were ready to pull up sleeves and beat the crap out of one another, and the moderators for cutting them off.
This was the opposite of the Democratic debates, where even the worst of the five was at least engaged in a conversation, even as he whined about not getting enough airtime, where, as former Baltimore mayor Martin O'Malley said, "On this stage you didn't hear anyone denigrate women, you didn't hear anyone make racist comments about new immigrants, you didn''t hear anyone speak ill of anyone because of their religious belief. What you heard was an honest debate of what will move us forward, to lead to a clean electric grid by 2050, and employ more of our people, rebuild our cities and towns, educate our children at higher and better levels, and include more people in the economic and social life in our country."
So, no, I wasn't watching politics play out on this stage. I was, indeed watching a horror show, of nine men and one woman fight, with no platform, no coherent plan for the future of our economy, our welfare, despite the debate being called "Your Money, Your Vote." If you want to know more about their policies (or anything about them) each of them would say, go to their websites, which they constantly plugged as if they just discovered the internet. Or should I say, the World Wide Web.
They didn't adhere to the time limits—not by a long shot—nor did they answer, or even seem to hear, the questions asked of them. Part of the blame goes to the moderators, who asked such inane questions as "What are your weaknesses," and even a question about fantasy football. Then again the moderators' job was unenviable, dispatched like new teachers into a classroom filled with entitled, rich kids whose parents have never taught them the meaning of "no." And let's be honest: The questions hardly matter. Candidates come with their own agendas—it's not like any one of these contenders were listening. They had their mental teleprompters on and their gloves off. But, as The New Republic's Jamil Smith tweeted last night to RNC Chairman Reince Priebus, who kvetched about CNBC, "Complaining about the referees is for the media, not the players. It's a loser move, @Reince."
I'll say this much: Rubio kept his eyes on the prize. He shook off Jeb!, who told the young senator to do the job he was elected to do (Rubio has missed more votes than any of his running mates in the Senate), and used it as another opportunity to take a jab at both the media and the Dems (President Obama, Bob Graham, and John Kerry). Rubio came in ready for a fight, determined to leave there a winner. And he did. Not with a brilliant platform, but by appearing the most mediagenic among a stage full of blubberers.
But you know who is going to win the election next November? They do. They told us with fear in their hearts: that woman, that terrible woman who is more threatening and dangerous than melting ice caps and Iran and socialism and ISIS all rolled in to one. In other words, the GOP's idea of a true American horror story.
2016 Presidential Election Ben Carson Donald Trump GOP Hillary Clinton jeb bush Mainstream Media Marco Rubio Politics
Kera Bolonik is DAME's editor-in-chief. Her essays, features, and criticism have appeared in national magazines and anthologies. She's currently at work on a nonfiction book entitled GULLIBLE, forthcoming from Dey Street/HarperCollins.
Find her @KeraBolonik
More by Kera Bolonik
I Just Can't Quit You, 'Desert Hearts'
Can Samantha Bee and John Oliver Save the 2016 Election
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redpajama_set_name: RedPajamaCommonCrawl


text:
MUSIC 445 A
MUSIC 445 A: Selected Topics In Ethnomusicology
Sound Archiving in Ethnomusicology
University of Washington, Winter 2016, Music 445 A (3 credits)
Thursdays, 1:30-3:50, Music Bldg. 27
Laurel Sercombe (julius@uw.edu) and John Vallier (vallier@uw.edu), co-instructors
Office Hours: By appointment
Class dates: Jan. 7, 14, 21, 28; Feb. 4, 11, 18, 25; March 3, 10
Final project due – Thursday, March 17, 5:00 p.m. in Canvas
The development of sound recording technology in the 1890s revolutionized scholarly research in music. With the establishment of the first sound archives at the turn of the 20th century, the link between the comparative study of the world's music and sound archiving was firmly established. This course will cover both historical and contemporary approaches to media archiving, with an emphasis on the curation of ethnographic recording collections. In addition to lectures and discussion, the class will offer hands-on experience with a variety of analog and digital audiovisual technologies, tools, and best practices. Other topics to be covered include copyright and ethics, digital access and preservation, description, and "green" archiving.
REQUIREMENTS & GRADING
Readings – All readings are freely available on the Canvas course site.
One in-class presentation based on reading assignments (10-15 minutes).
Weekly reflections (about 300 words) due in Canvas on Wednesday by 11:59 pm.
One-page written description of proposed project due in Canvas by 1:30 pm January 28 (class Week 4); bring paper copy of proposal to class that day.
Final project due in Canvas on March 14 by 5pm; oral presentation based on your final project due in class during Weeks 9 and 10.
Grading will be based on the following formula:
Class attendance, participation, and weekly reflections - 20%
In-class presentation - 20%
Final project proposal - 10%
Final project - 50%
January 7 (Week 1) - Introduction to Ethnomusicological Sound Archives
Student introductions
Instructor introductions & archive stories
Lecture: Introduction to Ethnomusicological Sound Archives (Laurel)
Assign readings for in-class presentations
January 14 (Week 2) – History of Sound Recording
Guest: John Gibbs (Special Projects Librarian, UW Music Library)
John's website is one of the best sources for on-line information related to
sound archiving: http://guides.lib.washington.edu/AudioPreservation
Readings for discussion:
Seeger, Anthony. "The Role of Sound Archives in Ethnomusicology Today," Ethnomusicology, Vol. 30, No. 2 (Spring/Summer, 1986), pp. 261-276.
Landau, Carolyn & Janet Topp Fargion. "We're all Archivists Now: Towards a more Equitable Ethnomusicology," Ethnomusicology Forum, Vol.21, No. 2 (August 2012), pp.125-140.
Maguire, Marsha. "Description of Audio Recordings," Chapter 5 in ARSC Guide to Audio Preservation, Sam Brylawski, Maya Lerman, Robin Pike, and Kathlin Smith, eds. Association for Recorded Sound Collections, Issuing Body. CLIR Publication No. 164.
January 21 (Week 3) – Audio Characteristics, Deterioration, and Preservation
Guest: Gary Louie (Media Maintenance Technician, UW School of Music)
Hands-on ethno archive experience (Week 1) - Ethno Archives and mediArcade
Discussion of issues related to the digital preservation of and access to archival materials, including an introduction to digitization standards and best practices as defined by the International Association of Sound and Audiovisual Archives (TC-04, 2nd edition, 2009).
IASA Technical Committee. "Key Digital Principles," in Guidelines on the Production and Preservation of Digital Audio Objects (TC-04, 2nd edition, 2009), Ch. 2.
Peoples, Curtis and Marsha Maguire. "Preserving Audio," Chapter 1 and Behl, Harrison. "Audio Formats: Characteristics and Deterioration," Chapter 2 in ARSC Guide to Audio Preservation, Sam Brylawski, Maya Lerman, Robin Pike, and Kathlin Smith, eds. Association for Recorded Sound Collections, Issuing Body. CLIR Publication No. 164.
January 28 (Week 4) – Audio Appraisal, Maintenance, and Description
Written description of proposed project due in class today
Lerman, Maya. "Appraisals and Priorities," Chapter 3 and Arton, Carla. "Care and Maintenance," Chapter 4 in ARSC Guide to Audio Preservation, Sam Brylawski, Maya Lerman, Robin Pike, and Kathlin Smith, eds. Association for Recorded Sound Collections, Issuing Body. CLIR Publication No. 164.
February 4 (Week 5) – Audio Preservation, Reformatting and Storage
Guest (via Skype): Alan Burdette (Director, Archives of Traditional Music, Indiana University) - The Media Digitization and Preservation Project at Indiana University
Chase, William. "Preservation Reformatting," Chapter 6 and Lacinak, Chris. "What to Do After Digitization," Chapter 7 in ARSC Guide to Audio Preservation, Sam Brylawski, Maya Lerman, Robin Pike, and Kathlin Smith, eds. Association for Recorded Sound Collections, Issuing Body. CLIR Publication No. 164.
February 11 (Week 6) – Contracts, Copyright, and Ethics
Guest: Chris Heaney (Ph.D. student, UW Information School)
Butler, Brandon. "Audio Preservation: The Legal Context," Chapter 8 in ARSC Guide to Audio Preservation, Sam Brylawski, Maya Lerman, Robin Pike, and Kathlin Smith, eds. Association for Recorded Sound Collections, Issuing Body. CLIR Publication No. 164.
Protocols for Native American Archival Materials - http://www2.nau.edu/libnap-p/protocols.html
Seeger, Anthony. "Traditional Music Ownership in a Commodified World," in Music and Copyright (2nd ed., 2004), Ch. 9.
February 18 (Week 7) - Video and Film
Guests: Andrew Weaver (Digital and Archival Media Technician, UW Libraries) and Hannah Palin (Film Archives Specialist, Special Collections, UW Libraries)
Bromberg, Nicolette, Hannah Palin, and Libby Burke. Washington State Film Preservation Manual: Low-cost & No-cost Suggestions to Care for Your Film (2004).
Bensinger, Charles. Chapters 2, 3, 4, and 6 of The Video Guide. 2d ed. Santa Barbara, Calif.: Video-Info Publications, 1979.
February 25 (Week 8) – Critical Concerns
Guest: Hiromi Lorraine Sakata (Professor Emerita, UCLA)
Sakata, Sercombe, and Vallier. "Radio Afghanistan Archive Project: Building Capacity, Averting Repatriation." Unpublished chapter on Radio Afghanistan. Submitted for review at Oxford University Press.
Fargion, Janet Topp. (2012). Connecting with Communities: Building Sustainable Models for Audiovisual Archiving into the Future. In Ethnomusicology in East Africa: Perspectives from Uganda and Beyond (pp. 49-59). Kampala, Uganda: Fountain.
Optional: Davis, Casey. "PROJECT_ARCC: Archivists Responding to Climate Change."
March 3 (Week 9) – In-class presentations (Week 1)
March 10 (Week 10) - In-class presentations (Week 2) (Last class!)
Monday, March 14 - Projects due
The syllabus page shows a table-oriented view of the course schedule, and the basics of course grading. You can add any other comments, notes, or thoughts you have about the course structure, course policies or anything else.
To add some comments, click the "Edit" link at the top.
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