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Mansouri-Najand, L., Kianpour, M., Sami, M., Jajarmi, M. (2015). Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran. Veterinary Research Forum, 6(3), 223-226.
Ladan Mansouri-Najand; Mehrnoush Kianpour; Masoud Sami; Maziar Jajarmi. "Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran". Veterinary Research Forum, 6, 3, 2015, 223-226.
Mansouri-Najand, L., Kianpour, M., Sami, M., Jajarmi, M. (2015). 'Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran', Veterinary Research Forum, 6(3), pp. 223-226.
Mansouri-Najand, L., Kianpour, M., Sami, M., Jajarmi, M. Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran. Veterinary Research Forum, 2015; 6(3): 223-226.
Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran
Article 7, Volume 6, Issue 3, Summer 2015, Page 223-226	 PDF (885.89 K)
Ladan Mansouri-Najand 1; Mehrnoush Kianpour2; Masoud Sami1; Maziar Jajarmi3
1Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
2Graduate Student, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
3Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Listeria monocytogenes as one of the most important pathogen in public health concerns is transmitted through consumption of contaminated food. The pathogen has been considered as a potential source of contamination of raw milk and dairy products. This research was aimed to investigate prevalence of L. monocytogenes in raw milk in Kerman region. In the summer of 2011, a total number of one hundred raw milk samples were collected from bulk tanks of some dairy farms and tested foriap and actA genes using polymerase chain reaction. Among the 100 samples, five isolates (5.0%) were detected as L. monocytogenes based on phenotypic and genotypic characteristics. Considering the low frequency of L. monocytogenes in this study, raw milk cannot be omitted as a potential source of food contamination for the population of the region. To achieve more accurate isolation, identification and control of L. monocytogenes in raw milk, it is suggested that new standard laboratory methods be implemented as well as biosafety outreach programs, management techniques and education.
Food Safety; Kerman; Listeria monocytogenes; Raw Milk; Iran
Listeria monocytogenes is one of the most important pathogens in public health concerns.1 This type of pathogen is transmitted through food which is particularly challenging for people with immune problems.2,3 Listeriosis (Listeriainfection) is relatively rare but statistics show that mortality rates can be high (up to 30.0%).4 The major means of transmission of L. monocytogenes to humans and animals is through consumption of contaminated food.5 The pathogen can be transmitted through various foods such as meat and meat products, fish and fish products, milk and dairy products (raw milk, soft cheeses etc.), vegetables and minimally processed food.1,5
There have been attempts to regulate amounts of L. monocytogenes in foods. In the USA, there should not be a bacterium in each 25 g of food.6 In Europe, this agentmust not be above 100 CFU g-1 during the shelf life of food and there should be no L. monocytogenes in products that bacterium may grow in them.7
Listeria monocytogenes can grow and remain in raw milk in a broad range of temperatures, low pH and high salt concentration.8,9 Therefore, the pathogen has been found in sheep, goat and cow milk and is considered as a potential source of contamination of raw milk and dairy products, and outbreaks subsequently.10,11 Incidence of the pathogen has been reported at different rates (3.4% to 6.0%) in raw milk that can be contaminated from infected feces and milk.12,13
There are various methods for rapid detection of L. monocytogenes, one of which is polymerase chain reaction (PCR). It detects virulent genes in a food sample. Therefore, a number of important virulent genes are plcB (encoding phospholipase C), hly (listeriolysin O), prfA (transcriptional regulatory protein), mpl (metalloprotease), actA (Actin assembly-inducing protein A) and iap (Invasion associated protein).14,15
The region of Kerman in southeastern Iran, with a population of more than 700,000 is the case study used to test the prevalence of pathogens that present a concern to public health. In this city, there are several big and small suppliers of milk and milk products. This research was aimed to investigate prevalence of L. monocytogenes in raw milk in this region. In previous years Listeria had been considered as an important foodborne pathogen to threaten public health by many related organizations such as International Dairy Federation (IDF). These tests on milk samples in Kerman province are the first under Iran's membership of the IDF.
Tests were done in the summer of 2011. A total number of one hundred raw milk samples were collected in sterile tubes from bulk tanks of some dairy farms in Kerman province. Once samples had been collected they were immediately transferred to the laboratory in conditions of 4 ˚C.
For each 25 mL sample of milk, 225 mL Listeria enrichment broth (Merck, Darmstadt, Germany) was added and then incubated at 30 ˚C. After 48 hr, 0.1 mL of enriched broth was cultivated on PALCAM Listeria selective agar medium (Merck) containing special supplements, at 37 ˚C for 48 hr. Listeria colonies were detected and confirmed by Gram staining, catalase production, oxidase activity and mobility tests. For identification of species, MicrogenTM Listeria-ID System (Microgen Bioproducts Ltd., Camberley, UK) was used according to the instructions. So Listeria isolates were evaluated in asculin hydrolysis, hemolysis, mannitol, xylose, arabitol, ribose, rhamnose, trehalose, tagatose, glucose-1-phosphate, methyl-D-glucose and methyl-D-mannose fermentation to identify species: L. monocytogenes, L. ivanovii, L. seeligeri, L. inocua, L. welshimeri and L. grayi.
Also, artificially contaminated milk samples were prepared in serial dilution of bacterium. An overnight cultivation of L. monocytogenes ATCC 7644 was prepared in brain-heart infusion broth (BHI; Merck). The bacterium was diluted in raw milk (10-1 to 10-15) and finally, 0.1 mL of each dilution was cultured in three plates of PALCAM agar (Merck) for 24 hr at 37 ˚C.
DNA extraction was done by the boiling method, 3 to 4 pure colonies were suspended in 1 mL distilled water and centrifuged at 14000 rpm for 1 min. Supernatants were eliminated and the remains was washed using by 500 µL NaCl 0.85%. Tubes were boiled at 100 ˚C for 10 min and then immediately placed on ice. Strains were subjected to detecting iap and actA genes for duplex-PCR using primers described previously (Table 1).16,17 Total volume of PCR amplification reaction was 25 μL containing: 10 μLDNA template, 0.2 mM of each primer, 0.2 mM of each dNTP (Cinnagene, Tehran, Iran), 2.5 mM MgCl2 (Cinnagene), 2.5 μL 10x PCR buffer (Cinnagene), 1 unit Taq DNA polymerase(Cinnagene) and distilled water up to reaction volume. Also, the thermal program was 95 ˚C for 5 min (Initial denaturation), 95 ˚C for 1.5 min (Denaturation), 46 ˚C for 80 sec (Annealing), and 72 ˚C for 2 min (Elongation) that 35 cycles were repeated from the second step. Finally, terminal extension was performed at 72 ˚C for 7 min. Electrophoresis was done with 5 μL PCR products on 1.5% agarose gel for 75 min at 100 V in tris borate EDTA (TBE; Cinnagene) buffer (90 mM trisbase, 90 mM boric acid, 20 mM EDTA, pH 8.0) containing 0.5 μg mL-1 ethidium bromide to visualize the amplicons. The electrophoresed gel was analyzed by UV trans-illumination. Listeria monocytogenes ATCC 7644 strain and distilled water was used as the positive and no-template negative controls, respectively, and specific bands were compared with 50-bp DNA ladder (Vivantis, Selangor, Malaysia).
Table 1. Primers used in the PCR reactions.
Tareget
Sequence 5'-3'
actA-F
GTGATAAAATCGACGAAAATCC
400 or 300 bp
Vazquez-Boland et al. 16
actA-R
CTTGTAAAACTAGAATCTAGCG
GGGCTTTATCCATAAAATA
453 bp
Manzano et al. 17
TTGGAAGAACCTTGATTA
Among the 100 samples, five isolates (5.0%) were detected as L. monocytogenes based on phenotypic characteristics. Those black with black sunken colonies in PALCAM Listeria selective agar mediums were Gram positive, catalase-positive and oxidase-negative. Also, these isolates possessed β hemolysis in sheep blood agar and tumbling motility at 25 ˚C and 37 ˚C. These strains were confirmed by MicrogenTM Listeria–ID System as L. monocytogenes: asculin hydrolysis, hemolysis, arabitol, rhamnose, trehalose, methyl-D-glucose and methyl-D-mannose fermentation was positive but mannitol, xylose, ribose, tagatose and glucose-1-phosphate fermentation was negative. All five Listeria strains were identified as L. monocytogenes and showed presence of iap and actA genes in PCR. In addition, five colonies were raised from 0.1 mL belonging to 10-13 dilution of bacterium in artificially contaminated milk samples. Thus, this method was determined as sensitive to 50 CFU mL-1.
This study determined that prevalence of L. monocytogenes in bulk tank, raw milk samples taken in the Kerman region, was low (5.0%). Results from tests in other areas in Iran have been reported as follows; 20% in Esfahan, 4.0% in Mashhad, 1.7% and 3.3% from two different dairy farms northern Iran.18-20 There have also been reports on prevalence of L. monocytogenes in milk samples from USA determined at 6.5%, Swedish dairy farm 1.0%, Morocco 5.9%, Latvia 1.4% and Syria with a considerably higher rate (41.6%).21-25
Results reported from the tests in this study are in agreement with reports on samples from Mashhad, east northern Iran and Latvia, while the rates of prevalence determined by tests in Esfahan, USA, Morocco and Syria were relatively higher and those in Sweden were lower.18-20 The variable rate of prevalence depends to some extent on hygiene, dairy management, the number of animals on a farm, season, geographical characteristics of a region, sampling technique and laboratory detection method.19,26 The most important risk factors of L. monocytogenes contamination of raw milk include defective disinfection of teats before milking, lack of correct management of barn and silage, insufficient hygiene practice in the environment and a low level of cleanliness among cows.27 Milking system and hygienic control of milking are considerations that have a considerable statistically relationship with risk of Listeria persistent in bulk tank milk.28 Therefore, the most important challenge during milking is exogenous contamination of milk with fecal material due to bad practice of hygiene standards.29 There are also challenges of good practice during transportation and storage of milk that need consideration.30,31
Although, prevalence of L. monocytogenes in this research was low, the importance of pasteurization and processing of dairy products (especially raw milk) should not be overlooked.32 Persons who are related to dairy farms such as producers and workers are at risk of oral infection by contaminated raw milk.25
In this study, all five isolates identified as L. monocytogenes were also positive for iap and actA genes. There is differences in the level of contamination with Listeria speciesbetween traditional foods.10 Proteins coded by iap gene are present in Listeria spp., however, they are different in each species and can be used for diagnostic targets by PCR.33 The actA gene is also appropriate for epidemiological approaches. This PCR-based assay can be considered as a specific method for recognize the DNA from different L. monocytogenes strains of raw milk, but it needs to more investigations.
In conclusion, considering the low frequency of L. monocytogenes in this study, raw milk cannot be omitted as a potential source of food contamination for the population of the region. It is recommended that a larger sample size should be employed selected from more dairy farms in order to make better and more precise judgment on pre-valence of L. monocytogenes. Thus, to achieve more accurate isolation, identification and control of L. monocytogenes in raw milk, it is suggested that using new standard laboratory methods be implemented as well as biosafety outreach programs, management techniques and education.
Adzitey F, Huda N. Listeria monocytogenes in foods: Incidences and possible control measures. Afr J Microbiol Res 2010; 4(25):2848-2855.
Schlech WF. Foodborne listeriosis. Clin Infect Dis 2000; 31:770-775.
Liu D. Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Med Microbiol 2006; 55:645-659.
Ireton K. Listeria monocytogenes. In: Chan VL, Sherman PM, Bourke B (Eds). Bacterial genomes and infectious diseases. 3rd ed. Totowa, USA: Humana Press Inc 2006; 125-149.
Ramaswamy V, Cresence VM, Rejitha JS, et al. Listeria: Review of epidemiology and pathogenesis. J Microbiol Immunol Infect 2007; 40:4-13.
Shank FR, Elliot EL, Wachsmuth IK, et al. US position on Listeria monocytogenes in foods. Food Control 1996; 7: 229-234.
EFSA (European Food Safety Authority), 2010. The Community Summary Report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in the European Union in 2008. EFSA J 2010; 8(1):1496.
Gahan CGM, O' Driscoll B, Hill C. Acid adaptation of Listeria monocytogenes can enhance survival in acidic foods and during milk fermentation. Appl Environ Microb 1996; 62:3128-3132.
Kim J, Schmidt KA, Phebus RK, et al. Time and temperature of stretching as critical control points for Listeria monocytogenes during production of mozzarella cheese. J Food Protect 1998; 61:116-118.
Rahimi E, Momtaz H, Sharifzadeh A, et al. Prevalence and antimicrobial resistance of Listeria species isolated from traditional dairy products in Chahar Mahal and Bakhtiary, Iran. Bulg J Vet Med 2012; 15(2):115-122.
De Buyser ML, Dufour B, Maire M, et al. Implication of milk and milk products in foodborne diseases in France and in different industrialized countries. Int J Food Microbiol 2001; 67:1-17.
Hunt K, Drummond N, Murphy M, et al. A case of bovine raw milk contamination with Listeria mono-cytogenes. Irish Vet J 2012; 65(13):1-5.
Ryser ET, Marth EH. Listeria, listeriosis and food safety. 3rd ed. New York, USA: Marcel Dekker Inc. 1999; 359-503.
Hein I, Klein D, Lehner A, et al. Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay. Res Microbiol 2001; 152:37-46.
Longhi C, Maffeo A, Penta M, et al. Detection of Listeria monocytogenes in Italian-style soft cheeses. J Appl Microbiol 2003; 94:879-885.
Vazquez-Boland JA, Kocks C, Dramsi S, et al. Nucleotide sequence of the lecithinase operon of Listeria mono-cytogenes and possible role of lecithinase in cell-to-cell spread. Infect Immun 1992; 60(1):219-230.
Manzano M, Cocolin L, Cantoni C, et al. A rapid method for the identification and partial serotyping of Listeria monocytogenes in food by PCR and restriction enzyme analysis. Int J Food Microbiol 1998. 21; 42(3):207-12.
Shahbazi AM, Rashedi M, Sohrabi R. Comparative contamination of Listeria monocytogenes in traditional dairy products in Esfahan province, Iran. Afr J Microbiol Res 2013; 7(16):1522-1526.
Jami S, Jamshidi A, Khanzadi S. The presence of Listeria monocytogenes in raw milk samples in Mashhad, Iran. Iran J Vet Res 2010; 11(4):363-367.
Mahmoudi MM. Occurrence of Listeria monocytogenes in raw milk and dairy products in Noorabad, Iran. J Anim Vet Adv 2010; 9(1):16-19.
Van Kessel JS, Karns JS, Gorski L, et al. Prevalence of Salmonellae, Listeria monocytogenes, and Fecal Coliforms in bulk tank milk on US dairies. J Dairy Sci 2004; 87:2822-2830.
Waak E, Tham W, Danielsson-Tham ML. Prevalence and fingerprinting of Listeria monocytogenes strains isolated from raw whole milk in farm bulk tanks and in dairy plant receiving tanks. Appl Environ Microbiol 2002; 68(7):3366-3370.
El Marnissi B, Bennani L, Cohen N, et al. Presence of Listeria monocytogenes in raw milk and traditional dairy products marketed in the north-central region of Morocco. Afr J Microbiol Res 2013. 7(5):87-91.
Konosonoka IH, Jemeljanovs A, Osmane B, et al. Incidence of Listeria spp. in dairy cows feed and raw milk in Latvia. ISRN Vet Sci 2012; 1-5.
Al-mariri A, Abou Younes A, Ramadan L. Prevalence of Listeria spp. In raw milk in Syria.Bulg J Vet Med 2013; 16(2):112-122.
Jayarao BM, Henning DR. Prevalence of foodborne pathogens in bulk tank milk. J Dairy Sci 2001; 84(10):2157-2162.
Sanaa M, Poutrel B, Menard JL, et al. Risk factors associated with contamination of raw milk by Listeria monocytogenes in dairy farms. J Dairy Sci 1993; 76:2891-2898.
Vilar MJ, Yus E, Sanjua´n ML, et al. Prevalence of and risk factors for Listeria species on dairy farms. J Dairy Sci 2007; 90:5083-5088.
Sanaa M, Audurier A, Poutrel B, et al. Origin of bovine raw milk contamination by Listeria monocytogenes. In Proceedings: Symposium on bacteriological quality of raw milk. Wolfpassing, Austria. 1996; 84-88.
Bemrah N, M Santa, MH Cassin, et al. Quantitative risk assessment of human listeriosis from consumption of soft cheese made from raw milk. Prev Vet Med 1998; 37:129-145.
Atil E, Ertas HB, Ozbey G. Isolation and molecular characterization of Listeria spp. from animals, food and environmental samples. Vet Med-Czech 2011; 56(8): 386-394.
O' Donnell ET. The Incidence of Salmonella and Listeria in raw milk from farm bulk tanks in England and Wales. Int J Dairy Technol 1995; 48:125-129.
Vazquez-Boland JA, Kuhn M, Berche P, et al. Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 2001; 14:584-640.


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Don't mention 'K' country: Bitcoin Magazine's YouTube restored after ban
After Bitcoin Magazine's YouTube channel was temporarily shut down without prior warning, the livestream's hosts suggested that mentions of "Kazakhstan" may have been flagged by the platform's algorithm.
Bitcoin Magazine's YouTube channel was restored around three hours after being shut down, with the publication attributing the short ban to the YouTube algorithm flagging the word "Kazakhstan."
In a Jan. 12 Twitter post, Bitcoin Magazine noted that its YouTube account with 56,600 followers was banned in the middle of a livestream with no prior warning from the platform.
"Our @YouTube with 60,000 followers just got BANNED mid-livestream with no warning. DELETED. When will the aggression against #Bitcoin content end?"
The livestream was focused on topics relating to Elon Musk, Jack Dorsey, Bitcoin (BTC) mining and the recent internet blackout in Kazakhstan that was reportedly initiated by the government in response to mass protests over surging fuel prices in the nation.
Bitcoin Magazine stated it wasn't entirely sure what grounds YouTube had used to ban its channel, but did confirm that its account had been restored an hour after it had submitted an appeal, suggesting that Youtube had realized its error.
In a live broadcast after the reinstatement, host Alex Mcshane noted that the panel was discussing the internet blackout's effect on the BTC mining hash rate without saying anything controversial, but was using a set of "algorithmically and politically charged words" which may have triggered the automated shutdown:
"I wanna talk about what happened, without triggering it again […], we were talking about a certain politically charged country that starts with a 'K'."
Bitcoin Magazine also shared a post earlier today noting the initial response from YouTube regarding the ban, with the Google-owned platform stating that "content that encourages illegal activities or incites users to violate YouTube's guidelines is not allowed."
"We may allow depictions of such activities if they are educational or documentary in nature and don't help others imitate them," the response added.
Despite its content policy, current searches on YouTube still yield results showing multiple live streams using the identity and video content of popular figures such as MicroStrategy's Michael Saylor to promote dodgy websites and supposed "crypto giveaway" scams.
Either Michael Saylor does a lot of live streams or some vids are dodgy: YouTube
Related: Key on-chain metric shows Bitcoin miners in 'massive' BTC accumulation mode
Commenting on the ban in the r/CryptoCurrency subreddit, user u/Setl1less highlighted the hypocrisy, arguing that "Youtube has made it a habit of taking down prominent informative accounts" while allowing scams to operate freely.
Related Topics:Don't mention 'K' country: Bitcoin Magazine's YouTube restored after ban
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redpajama_set_name: RedPajamaCommonCrawl


A number of Tradies Software Apps are being developed for the Australian Trades Services companies fondly known as 'Tradies' in Australia. However, are they really making the difference for Tradie Business owners? At i4Tradies, we are proud to say it does in many ways. Here is one example of many that we can claim as our unique value proposition.
With mobile technology becoming an inevitable part of our everyday lives, its adoption has been on the rise even among individuals suffering from physical disabilities. Running a successful Windows Cleaning Service, Melbourne based tradie, Walter Kadiki not only loved his business but was also exceptionally good at it. However, for Walter, being able to effectively communicate with his employees and customers remained a challenge due to being hard of hearing.
"I felt stuck in the slow cycle of desktop based email communications to receive and respond to customer requests," Walter recalls. "Hiring someone to interpret communications was way out of question because of the costs and time delays," he added.
Walter decided to do something about the problem before he begins to lose footing compared to his other mates that appeared much more responsive to their customers' and business needs.
That's when Walter learned about i4Tradies, a mobile based Tradies Software Job Management platform that helped him remove the communication gap with his employees and customers and the disadvantage he previously experienced in following up new business leads.
Previously Trades Services businesses run by hard of hearing individuals used to rely merely on emails and SMS to take orders or respond back. While it used to be a good way to communicate, it was not only slow but also less preferred by time-strapped tech savvy customers.
i4Tradies Tradies Software Job Management platform, a cloud based SaaS, offers seamless two way communication through a single platform of choice for everyone alike. Customers dealing with a deaf Tradie do not need a different app than the one they are using to interact with a hearing Tradies.
Moreover, the hard of hearing Tradie can receive and respond to customer requests much in the same way his hearing competitors can, thereby leveling the playing field for them.
Individuals who are hard of hearing have a heightened sense of visual perception and hence greater attention to detail.
A telephonic job request can only be so descriptive and detailed. On the other hand, i4Tradies allows customers to send a visual description of the job through photos along with text, thereby removing any ambiguities hard of hearing Tradies may have with respect to the job.
From responding to customer inquiries to keeping them up to date on job progress, invoicing them and chasing them for payments, a tradie's job can be much more than the actual job itself!
However, for a hard of hearing individual, the entire process can be quite intimidating with their inability to communicate as effectively and as frequently as required.
With i4Tradies automated customer and employee interactions, hard of hearing trades services business owners can not only share job details and allocate them to their employees right from the app but they can also keep the customer in the loop as job progress from one stage to the next without having to communicate directly.
'Now with use of i4Tradies, we can integrate our hard of hearing staff so they can competently do their plumbing jobs and never shy away from communicating with our clients. In fact we have observed that it has given our hard of hearing plumbing staff a boost since we adopted i4Tradies business process platform as it communicates via SMS and/or the smart apps notifications," says Saia Latu, Business Manager at Fluid Plumbing.
Hard of hearing Tradies can feel disadvantaged when it comes to securing new leads and communicating well with their customers.
However, the advancement in mobile based technology driven solutions such as i4Tradies – Tradies Software (a SaaS cloud based Platform), helps level the playing field for hard of hearing Tradies with others. The Tradies software helps deaf Tradies feel more confident when communicating with their customers and their employees and achieve more in less time.


"The Last Frontier": An Interview with author Julia Assante, Ph.D.
hospice in the world, The Last Frontier by Julia Assante, Ph.D.
are a number of personal experiences with the dying and the dead.
I recently put some questions to her by e-mail.
Dr. Assante, many people say we shouldn't concern ourselves with what happens after death, but should live for today. How do you respond to them?
dying immeasurably easier and opens up the pathways for communication.
How did you become interested in the subject of life after death?
Have you always been a believer in an afterlife?
my childhood made it impossible to forget that there is an eternity.
explain how you acquired these gifts?
"First let me say that without exception everyone has these gifts.
future zones still exist now but can't be perceived.
are anchored. The individual personality never dies, even after death.
are becoming more spiritual. What are your thoughts on this?
focus on materialism and greatly hampered our intuitive faculties.
generations ago. The change during my lifetime is quite noticeable.
grow differently, wasting energy in worry and strife?
contact is normal transforms the way we die and the way we live.


Incredible blog lists here and there.
Cédric Pontet is a seasoned Lean and Agile practitioner, with more than 13 years of experience in software development. He really started focusing on Agile project management when he joined Agile Partner. Since 2005. Cédric has been involved in sizable projects, for several customers in sectors as various as "notaries" public sector, intellectual property management, banking & insurance, or post & telecom. He has been working on these projects first as a developer, and then as an architect and a team leader, gaining more and more experience and agility, while always keeping an eye on technology evolutions. Lately, Cédric has been spending more time coaching teams to start their agile transformation, evangelizing Lean and Agile values, in Luxembourg and its neighboring countries, not only for software development but beyond.


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I'm an 18 year old high school leaver who is in need of employment in order to move onto the Tertiary Level. I'm also a holder of 9 CXC subjects inclusive of English and Math. I am very hard working, enthusiastic and dedicated in everything that I do. I enjoy interacting with computers and people, therefore my interest in Office and Database Administration. I currently have two months of experience in office work, which was obtained over the summer and through previous voluntary services. In addition I am well-disciplined as well as having good decision making skills which was further developed from serving as Deputy Head Girl and Peer Counselor to my past High School.
Primary trained teacher seeks employment.
Intelligent, caring practical nurse with experience in caring for the elderly in nursing home seeks job opportunity to work in nursing home, private home or small doctor's office. Willing to work late on week days and on Saturdays to ensure the care and well-being of patient(s).
merchandiser at any given Company!
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Port of El Copano, 1722
The port of El Copano
Near Bayside, Refugio County
2008 Most Endangered List
The port of El Copano in Refugio County is one of 12 sites that Preservation Texas, Inc. has named to its tenth anniversary retrospective list of Texas' Most Endangered Historic Places.
Originally named to the 2008 list of Texas'Most Endangered Historic Places, it is designated as a lost site on the 2013 list. Preservation Texas officials announced the selections on the steps of the Texas State Capitol on Preservation Day, February 20. The annual announcement is part of Preservation Day activities organized by Preservation Texas. Advocates from all over the state come to participate in informational sessions, meet with legislators at the Capitol and get an in depth look at lessons to be learned from the sites named to the list.
"The 2013 list provides a retrospective for us," said Charlene Orr, president of Preservation Texas, Inc., a statewide partner of the National Trust for Historic Preservation. "We decided to focus on lessons learned during the last 10 years by highlighting success stories as well as losses and threatened sites that continue to need urgent attention." The 2013 list includes six saved sites, three threatened sites and three lost sites.
"Our six saved sites reflect the importance of collaboration between committed grassroots leadership and elected officials and city officials who realize the importance of historic preservation." she continued. "It's this powerful combination of resources that can make the difference in preserving a site or losing it."
"Inclusion of the port of El Copano is important because it illustrates some of the very real obstacles communities face in their efforts to preserve the historic resources of Texas," said Orr. "Sadly, the port is part of the history of our state that now is gone forever."
The port of El Copano was established in 1722 by the Spanish to service the missions and military garrisons at Refugio, Goliad, and San Antonio. Wharves, warehouse, and commercial establishments were built as seagoing vessels moved cargo and people. El Copano was the main port of entry for Irish immigrants during the 1820s and 1830s Empresario period. The port was also used during the Texas Revolution and the Civil War.
Bypassed by the railroad and hit by hurricanes, the port eventually declined. Most of the structures at El Copano were constructed of shellcrete masonry and there are two known cemeteries on the site.
The 10-foot bluff on which the port was built is eroding into the bay and only a few structures survive. Additionally, no public roads lead to the site and this has added to obscurity of the site. The site is owned by an estate and the multilple heirs have not been able to agree on plans to maintain the site. Currently, the heirs are unwilling to allow access to the site and have been accessible only through their attorney.


Probably you are one of those people that won't remember any birthday what so ever and need the help of Facebook to be able to remember important dates. If that is the case, you also probably never know what to write to wish a happy birthday. Your problems will be solved with Free E Cards, just pick the one that looks like the person you want to wish a Happy Birthday and send it with a nice message. I am sure the person will truly appreciate it and it will only take a couple of minutes.
Thanks to Facebook and even Skype, we have day notice of any birthday of a friends. I cannot even remember the time where you have to have all birth dates on your head so that you would forget any of them. But now, the question is: what to write other than "Happy Birthday"? perhaps something deeper in meaning or feeling and a little longer just to show your friends that you really care for them. To make your life easier take a look to the following birthday wishes and use them any time.


text:
Q: jquery not firing code inside datatables declaration i have the following code:
let checkedValues=new Array();
jq(document).ready(function() {
    table = jq('#example').DataTable( {
             "select":{
                "style": "multi"
             },
             "order":[[1,"asc"]],
             "dom": "Bfrtip",
             "bLengthChange":false,
             "ajax": '/en/users/api/get-users',
             "columnDefs": [
                    {
                        "orderable":false,
                        "className":"select-checkbox",
                        "targets":0,
                        "width":"5%"
                    },
                    {
                        "targets": -1,
                        "data": null,
                        "defaultContent": "<button type='button' class='btn btn-info smallPadding edit'>Edit</button>  <button type='button' class='btn btn-success smallPadding export'>Export</button>  <button type='button' class='btn btn-danger smallPadding delete'>Delete</button>"
                    },
                    ],
                    "buttons": [
                        {
                            "text": "Get selected data",
                            "action": function () {
                                table.rows( { selected: true } ).each(function() {
                                   var row = table.row(jq(this)).data();
                                   checkedValues.push(row[1]);
                                })
                                events.empty();
                                let count=1;
                                events.prepend( '<div>'+count+' row(s) selected</div>' );
                            }
                        }
                    ],
                  });
                  jq('#example .select-checkbox').on('click', function () {
                    alert(1);
                    var data = table.row( jq(this) ).data();
                    checkedValues.push(data[1]);
                });
            });

You'll see there is code jq('#example .select-checkbox').on('click', function () { which doesnt seem to take effect. I know it works because i can manually put it in my console and it operates fine.
Not sure what to do at this point. I also tried taking it out of the jq(document).ready
UPDATE:
i was able to get it working with a timeout but that seems like a hack.

A: You can move that code into an initComplete() function, so the click event will not be added until the DataTable has finished initializing:
"buttons": [...],
"initComplete": function( settings, json ) {
    jq('#example .select-checkbox').on('click', function () {
        alert(1);
    });
}

Reference here.

redpajama_set_name: RedPajamaStackExchange


This is the size and type of solder we recommend for surface mount soldering.
For a 7 min. how-to video with close-up, slow motion shots of proper technique, go here. The video and following guide also explain the importance of flux, how much wattage is needed, tip shape, and just how bad the fumes are. This is rosin-cored wire solder, with a "66" core of 44 flux--this means it's 3.3% by weight flux. It can also be purchased in smaller percentages, and while in general it's best to use the least amount of flux possible, for hobby and non-critical applications, ease of soldering is more important (see below for corrosion risk). This flux is classified as ROM1, which means Rosin based, medium activity, and halides added. 60/40 vs. 63/37 vs. silver bearing solder: 60/40 means 60% tin and 40% lead by weight. This explains the difference between the different types in detail. 63/37 is eutectic, has a slightly lower melting point, and "freezes" a bit faster. In mass production style wave soldering, this is worth the increase in cost, but not for hand soldering. Silver bearing solder has a bit of silver that many claim is better for surface mount soldering, stronger joints, and higher audio fidelity. Silver was originally added to keep solder from leeching silver off connections, not any of the above reasons. Stiffer silver joints may actually make joints less reliable because the stiffness stresses small component leads more when the board changes temperature. Amount: How far will an ounce go? In a rough experiment, we generously soldered 16, 16-pin chips, tinning the tip in between each one. Based on that usage, 1 oz would complete over 3000 joints. To our knowledge, CuriousInventor.com is the only place to purchase Kester solder in quantities less than a lb. The data sheet can be found here. Why buy this over RadioShack solder? First, the prices are comparable. Second, Kester is the Rolls-Royce of solder, and has decades of industrial use attesting to the quality of the solder and the flux contained in the wire. Now, we grew up using RadioShack solder for hundreds of projects, so we certainly aren't going to claim it doesn't work. However, like many things in RadioShack, the specs are unknown--just how active is the flux and how likely is it that the residues will corrode a circuit board? The quality of solder is mostly based on its flux, and whether or not there are any air gaps in the wire. Kester's manufacturing process is supposedly superior to others when it comes to avoiding gaps in small diameter wire. Kester 44 flux strikes a difficult balance between a very active flux (one that cleans oxides effectively and helps the solder to wick quickly, even on difficult surfaces like nickel), and one that won't corrode the board later. No-clean solder / flux: For non-critical applications, we recommend "standard" rosin flux that has been activated, but this recommendation only goes out to Kester brand 44 and 48 fluxes. Activated rosin flux makes soldering easier and faster, especially on oxidized or other difficult surfaces. And, the risk that the residues will cause corrosion is debatable at best. If you're building a project that will operate in humid, salty ocean air, consider cleaning the flux or using a no-clean type. But otherwise, it may not even be necessary to clean. From the Kester 44 spec sheet: "Kester 44 possesses excellent fluxing ability, the flux residue is non-corrosive and non-conductive under normal conditions of use. When exposed to an elevated temperature and humidity environment (38���C, 94% RH) for 72 hours, there is no evidence of corrosion caused by the flux residue. Throughout its many years of wide usage, 44 Rosin Flux has produced many billions of soldered connections. In all these billions of solder joints, involving the most delicate and critical of electrical and electronic components, there has never been an authentic instance of corrosion by the flux residue under normal conditions."


It's true. Active and engaged seniors live richer, healthier and more satisfied lives. That's why Aviva offers an exciting assortment of activities, events and programs to make sure there's always something fun to do – whenever you're ready to do it. From exercise and cooking classes to special trips, sporting events, happy hours, game and movie nights, and more, we believe in the happiness that comes from sharing activities with friends.


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The inaugural C&IT Awards featured winners including Line Up, Grass Roots and DRP Group- and we have the pictures.
C&IT kicked-off its inaugural Awards in spectacular style on Friday (4 November) at the Natural History Museum. The first batch of pictures, featuring the reception drinks, is here.
The pictures of the Award winners are here.


text:
Burger King Corporation és una cadena de restaurants de menjar ràpid nord-americà. Neix a Miami el 1954, quan dos joves emprenedors anomenats James McLamore i David Egerton funden una hamburgueseria basada en un concepte diferent del que existia i que s'ha mantingut fins avui: «Ens sentim orgullosos de servir als nostres clients les millors hamburgueses i una varietat d'altres productes saborosos i saludables, cuinats a foc. Això és el que som»

L'hamburguesa és el producte principal de Burger King, com el nom indica, sent el Whopper i el Big King els seus productes principals, li segueixen les amanides i menjars amb carn de pollastre. El 2000 la companyia va decidir ampliar la seva oferta i va crear un menú vegetarià. El 2 de setembre de 2010, l'empresa va ser adquirida per l'empresa brasilera 3G Capital. És patrocinador oficial del Getafe CF, equip de la Primera Divisió (Lliga BBVA) de futbol d'Espanya.

Referències

Enllaços externs
 Lloc oficial internacional de Burger King

Miami

Empreses d'alimentació dels Estats Units
Cadenes de locals de menjar ràpid
Empreses de Florida

redpajama_set_name: RedPajamaWikipedia


text:
9 Sound Design Tricks To Hack Your Listener's Ears
It can be deceptively easy to mix and master a track, but what happens to the sound when it leaves the speakers?
Our friends at Get That Pro Sound give a rundown on sound design techniques that will seduce your listeners' ears into hearing exactly what you want.
Sound Design Tricks 101
With using mere basic knowledge about psychoacoustic principles, you can find creative ways to bring your listeners a more powerful, clear, and 'larger than life' experience. By understanding how the hearing system interprets sounds, we can creatively and artificially recreate certain responses to particular audio phenomena, particularly EQ, compression and reverb.
For example, if you incorporate the natural reflex of the ear into the designed dynamics of the original sound by including a very loud hit, the brain will perceive the sound as "loud" (even when it's played back relatively quietly).
You've fooled the brain into thinking that the ear has closed down slightly in response to 'loudness'. The result? The experience of loudness is quite distinct from actual physical loudness.
1. The Haas Effect
Named after Dr. Helmut Haas (who first described it in 1949), this principle can be used to create an illusion of spacious stereo width…starting with just a single mono source.
Haas was studying how ears interpreted the relationship between originating sounds and their 'early reflections' within a space. His conclusion was that – as long as early reflections and identical copies of original sounds are heard less than 35ms after (and at a level no greater than 10dB louder than the original) – the two sounds will be interpreted as a single one.
The directivity of the original sound would be essentially preserved, but because of the subtle phase difference, the early reflections/delayed copy would add extra spatial presence to the perceived sound.
The Haas Effect in Practice
In a musical context, for thickening and/or spreading out distorted guitars (or any other mono sound source), it's a good trick to duplicate the part, pan the original to extreme right/left, and pan the copy to the opposite extreme.
You might also delay the copy by between about 10-35ms (every application desires a slightly different amount) by shifting the part back on the DAW timeline or inserting a basic delay plugin on the copy channel with the appropriate delay time dialed in. This tricks the brain into perceiving larger width and space while leaving the center wide open for other instruments.
You can also use this technique to pan a mono signal away from the busy center in order to avoid masking from other instruments. At the same time, you don't want to unbalance the mix by only panning to one side or the other. The answer lies in "Haasing it up" and panning your mono signal both ways.
Consider Using Slight Delays
Of course, there's nothing stopping you from slightly delaying one side of a real stereo sound. For example, you might want to spread your ethereal synth pad to epic proportions. Just be aware, however, that you'll also be making it that much more 'unfocused' as well. For pads and background guitars though, this is often entirely appropriate.
As you play with the delay time setting, you'll notice that too-short delays result in a pretty nasty out-of-phase sound. Meanwhile, too-long delays will break the illusion, and you'll start to hear two distinct and separate sounds. You're looking for something in between, which will sound just right and help you catch the space you want.
Find the Right Balance
Remember: The shorter the delay time, the more susceptible the sound is to unwanted comb filtering when the channels are summed to mono. This is something to consider if you're making music primarily for clubs, radio, or other mono playback environments.
You'll also probably want to tweak the levels of each side (relative to each other) to maintain the right balance in the mix and the desired general left-right balance within the stereo spectrum.
You can apply additional effects to one/both sides, like applying subtle LFO-controlled modulation or filter effects to the delayed side.
A word of caution: Don't overdo it. In a full mix, use the Haas Effect on one or two instruments, maximum. This helps you avoid unfocusing the stereo spread and being left with phasey mush.
2. Frequency Theory: How Masking Works
There are limits to how well our ears can differentiate between sounds that occupy similar frequencies of human hearing.
Masking occurs when two or more sounds sit in the exact same frequencies. Generally, the louder of the two will either partially or completely obscure the other, which then seems to 'disappear' from the mix.
Obviously, this is a pretty undesirable 'phenomenon,' and it's one of the main things to be aware of throughout the whole writing, recording, and mixing process. It's also one of the main reasons EQ was developed, which can be used to carve away masking frequencies during the mixing stage.
Our audio trick? Avoid masking problems during the writing and arranging stages by using notes and instruments that occupy their own frequency ranges.
Even if you've taken precautions, masking will still sometimes occur at the mix, and it's difficult to determine why certain elements sound different solo than they do in the full mix.
Although the root notes/dominant frequencies of the sound have the space they need, the sound harmonics (that also contribute to the overall timbre) appear at different frequencies. These may still be masked, which is a point where EQ might come to the rescue.
3. The Ear's Acoustic Reflex
As mentioned in the introduction, when confronted with a high-intensity stimulus, he middle ear muscles involuntarily contract. This decreases the amount of vibrational energy that transfers to the sensitive cochlea, which converts sonic vibrations into electrical impulses for processing by the brain. Basically, the muscles close to protect the more sensitive structures of the ear.
The brain interprets the dynamic signature of these reduced-loudness sounds, with the initial loud transient followed by immediate reduction when the ear muscles respond. The result? It still senses 'loud sustained noise'.
This principle is often used in cinematic sound design techniques and is particularly useful for simulating the physiological impact of massive explosions and high-intensity gunfire (without inducing hearing-damage lawsuits).
The ears' reflex to loud sounds can be simulated by manually playing with fine dynamics of sound. You can make that explosion appear quite loud by artificially shutting down the sound following the initial transient. The brain will immediately perceive it as louder and more intense than the sound actually is. This also works well for booms, impacts, and even drops in a club or electronic track.
4. Create Power and Loudness – Even at Low Listening Levels
If you take only one thing away from this article, hear this: The ears' natural frequency response is non-linear. More specifically, our ears are more sensitive to mid-range sounds than frequencies at the extreme high and low ends of the spectrum. We generally don't notice this, as we've always heard sound this way and our brains take the mid-range bias into account. It does, however, become more apparent during mixing, where relative levels of instruments (at different frequencies) change depending on the overall volume you're listening at.
Even though your own ears are an obstacle to achieving a perfect mix, there are simple workarounds to this phenomenon. You can also manipulate the ears' non-linear response to different frequencies and volumes in order to create an enhanced impression of loudness and punch in a mix – even when the actual listening level is low.
The Fletcher-Munson Phenomenon
This nonlinear hearing phenomenon was first written about in 1933 by researchers Harvey Fletcher and Wilden A. Munson and although the data and graphs they produced have since been improved upon, they were close enough that 'Fletcher-Munson' is still used as a shorthand phrase for everything related to 'equal loudness contours'.
Generally, you should be able to do the best balancing at low volumes (this also saves your ears from unnecessary fatigue). Loud volumes are generally poor for creating an accurate balance because, as per Fletcher-Munson, everything seems closer than it is.
Think About Your Audience
In certain situations (like mixing sound for films), it's better to mix at the same level and similar environment to where the film will eventually be heard.
This is why film dubbing theaters look like actual cinemas and are designed to essentially sound like them too.
The best mixes result from taking the end listener and their environment into account, not necessarily mixing something that only sounds great in a $1 million studio.
Cinema-scale mixing at Skywalker Sound
So, how do our ears' sensitivity to the mid-range manifest on a practical level? Try playing back any piece of music at a low level. Now gradually turn it up: As the level increases, you might notice that the 'mid-boost' bias of your hearing system has less of an effect, with the high- and low-frequency sounds seeming proportionally louder (and closer, which we'll go into in the next tip).
Given that extremely high and low frequencies stand out more when we listen to loud sound effects, we can create the impression of loudness at lower listening levels by attenuating the mid-range and/or boosting the high/low ends of the spectrum. On a graphic EQ, it would look like a smiley face, which is why producers talk about 'scooping the mid-range' to add weight and power to a mix.
How to Apply This Audio Trick
This trick can be applied in any number of ways, from treating the whole mix to some (careful) broad EQ at mixdown/mastering to applying a 'scoop' to just one or two broadband instruments or mix stems (i.e. the drums and guitars submix). As you gain experience and get your head around this principle (you might even already be doing it naturally), you can build your track arrangements and choices of instrumentation with an overall frequency dynamic – right from the beginning.
This is especially effective for styles like Drum & Bass, where amazingly impactful and rich-sounding mixes – with just a few elements that really work those high and (especially) low ends. The mid-range leveling can then act primarily as an indicator of a 'nominal' base level, made artificially low to enhance the emphasis on the massive bass and cut high-end percussion and distortion.
The same works well for rock too: Just listen to Butch Vig's production on Nirvana's "Nevermind" for a classic example of scooped mids dynamics.
Remember to be subtle: It's easy to overdo any kind of broad frequency adjustments across a whole mix. If in doubt, leave it for mastering.
5. Equal Loudness Part II: Fletcher-Munson Strikes Back
Of course, the inverse of the closer/louder effect on the ears' nonlinear response is also true, and equally useful for mixing purposes. To make things appear further away – instead of boosting – roll off the extreme highs and lows. This creates a sense of front-to-back depth in a mix, pushing certain supporting instruments into the imaginary distance and keeping the foreground clear for the lead elements.
This (and the previous trick) works because of how our ears interpret distance from a sound source by the amount of high- and low-frequency energy present (which is relative to broader mid-range content).
Our ears have adapted to take basic physics of our gaseous Earth atmosphere into account: beyond very short distances the further any sound travels, the more high-frequency energy (and extreme low-end to a slightly lesser extent) will dissipate as it travels through the atmosphere.
Put These Sound Design Techniques into Action
To push a sound further back in the mix, try rolling off varying amounts of higher frequencies and hear it recede behind the other elements. This is particularly useful for highlighting a lead vocal in front of a host of backing vocals (cut the BVs above around 10kHz, possibly boost the lead vocal in the same range). It's also a solid choice for EQing drum submixes to ensure the drums are punchy overall but not too in-your-face. A touch of reverb is also an option here, naturally.
6. Transients Appear Quieter Than Sustained Sounds
The human ear doesn't perceive short duration sounds as loud (or long) when they're at exactly the same level. This is referred to as RMS or 'Root Mean Square' response, a mathematical means of determining average signal levels. This is the key auditory principle behind how compression makes things sound louder and more exciting without actually increasing the peak level.
Compressors aren't as intuitively responsive as the human ear, but they're designed to respond in a similar way. Compressing the tails of sounds tricks the brain into thinking sound (like a drum hit) is significantly louder and punchier. But the peak level – the transient – has not changed. This is how compressors allow you to squeeze every ounce of available headroom out of your sounds and mix. Just be careful not to 'flatline' your mix with over-compression.
7. Reverb Early Reflection 'Ambience' For Thicker Sounds
If you combine part of the Haas Effect principle with our previous tip on sustained sounds, you'll already understand how adding early reflections from a reverb plugin can attractively thicken sounds.
Here, we're using it to essentially multiply and spread the initial transient attack portion of sound over a very short amount of time. By extending this louder part of the sound, we'll get a slightly 'thicker' sound, but in a very natural, ambient way that's easily sculpted and tonally fine-tuned with various reverb controls. With the distancing and diffusion effects of the long tail, you can retain the 'upfront' character of the sound.
8. Decouple a Sound from Its Source
A sound as it is produced and perceived in a final context is really not the same thing at all.
This principle is liberally exploited in movie sound effects design, where the best sound designers are able to completely dissociate the sonic qualities and possibilities of a sound from its original source. This is how Oscar-winning Ben Burtt came up with the iconic Star Wars lightsaber sound:
"I had a broken wire on one of my microphones, which had been set down next to a television set and the mic picked up a buzz, a sputter, from the picture tube – just the kind of thing a sound engineer would normally label a mistake. But sometimes bad sound can be your friend. I recorded that buzz from the picture tube and combined it with the hum [from an old movie projector], and the blend became the basis for all the lightsabers."
– Ben Burtt, in the excellent The Sounds Of Star Wars interactive book
The Start of Techno
The Roland TR-808 and TB-303 were originally designed to simulate real drums and real bass for solo musicians. The results were pretty shocking as they did sound like real instruments, but trailbreaking Techno producers like Juan Atkins and Kevin Saunderson perceived their potential to be more exciting than backing up a solo guitarist at a pub gig.
By misusing and highlighting what made them unique from the real thing, these pioneers turned loud bass into screaming acid resonance and tweaked the 808 kick drum to accentuate
its now signature 'boom'.
Johnny Cash's Signature Sound
Consider Johnny Cash's infamous track "I Walk The Line", which featured his technique of slipping a piece of paper between the strings of his guitar to create his own 'snare drum' effect. Apparently, Cash did this because snares weren't used in country music at the time, but he loved their sound and wanted to incorporate it. Coupled with the train-track rhythm and the imagery of trains and travel in the lyrics added a new dimension to the song – just one of the magical moments of studio recording.
The Ends Really Can Justify the Means
Whether you're creating a film sound effect or mixing a rock band, you don't have to settle for the raw or typical instrument sounds you started with. If you find that kitchen pans give you sounds that fit your track better than an expensive tuned drum kit, use them! If you discover pitched elephant screams are the perfect addition to your cut-up Dubstep bassline (it works for Skrillex), by all means, herd them in.
The only thing that matters is the perceived end result: No one's ears care how you got there. They'll be subliminally much more excited and engaged by sounds coming from an unusual source, even if said sounds take the place of a conventional instrument.
9. Sound Design: How to Make a Mix Sound Fuller
Our ears can have trouble deciphering where one sound ends and another similar one begins, but they're incredibly forgiving when it comes to layered sounds. When done carefully – even across wide ranges of the frequency spectrum – the separate components will 'read' as one big, textured sound.
This is basically how musical chords work, as well as a major principle behind expensive and lush Hollywood-style sound design. This is also true for complex, 'How did they do that?' effects in electronic music.
The Practical Art Of Motion Picture Sound discusses creating layered sound effects as though there were chords. A single effect is made up of several individual sounds/'notes' which occupy their own frequency range (this is key to avoid simply masking one sound with another). Adding your own timbre or dynamics all together provides a depth, texture, and power that's simply not possible with a single-source sound, no matter how much it's processed.
Use this technique to layer whole stacks of guitar or synth parts into towering walls of sound that can then also be spread across the stereo field with the Haas Effect. Build signature drum kits by layering frequency and timbral elements from multiple sources to create the ultimate Tech House kick or Dubstep snare drum. The possibilities for tricking our ears are endless!


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text:
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Our Former New Jersey Deputy Attorney General has handled hundreds of ABC cases. Let us put our experience to work for you.
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NJ Division of Alcoholic Beverage Control
Monmouth County Go-Go Bar Shut Down by NJ Division of Alcoholic Beverage Control
According to a recent article, the New Jersey Division of Alcoholic Beverage Control suspended the liquor license privileges of go-go bar Fantasies on Route 35 in Keyport. Not only will the liquor license owned by Fantasies be suspended for a total of 135 days, but the licensee will be required to pay a fine totaling... Read More
NJ ABC Seeks to Revoke Liquor License for Trump's Golf Course
According to reports, the Division of Alcoholic Beverage Control in New Jersey issued a formal Notice of Charges to Trump National in Colts Neck, New Jersey. The basis for these charges date back to a 2015 incident involving the service of alcoholic beverages to an intoxicated patron who subsequently was involved a fatal motor vehicle... Read More
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Pittsburgh is falling out of love with Uber's self-driving cars
Rob LeFebvre, @roblef
Nathan Ingraham/Engadget
Pittsburgh was the initial test city for Uber's self-driving car initiative. While our experience in one of the autonomous vehicles was thankfully pretty safe, it wasn't long before reports of accidents and wrong-way driving began to surface during the first month of the operation. Nine months later, the relationship continues to sour, according to a report in the New York Times. The things Uber promised in return for the city's support — including free rides in driverless cars, backing the city's $50 million federal transportation grant and jobs for a neighborhood nearby Uber's testing track — have not materialized. The situation was an issue during the mayoral primary, too, with critics calling out incumbent Bill Peduto for not getting these agreements in writing from the ride-sharing company.
Uber responded to the criticism, telling the Times that it has created 675 jobs in the greater Pittsburgh area and has contributed to local organizations. However, one high-profile pastor near Uber's test track said that he'd given Uber a list of potential candidates for jobs at the site, but was told to send them through the regular job portal. According to the Times, none of these individuals, including a mapping engineer and other technicians, have been hired. Uber told the Times that while it was open to a deal with the city, it was still waiting for a draft proposal from local officials.
Uber's problems don't stop at the Pittsburgh city line, of course. The company continues to fight a lawsuit around alleged stolen tech, its drivers are dissatisfied with new fee structures and even Elon Musk turned them down for a partnership in self-driving cars. The company also had to halt its autonomous program in Tempe, Arizona after a car crash. Ford has reportedly invested $1 billion in a Pittsburgh-based self-driving car startup, which could meet more of the city's need for written agreements and data sharing. The current Uber initiative in Pittsburgh may have raised the profile of the city as a technology-friendly town, but this new falling out is yet another example of the turmoil surrouding the company.
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Hi, I'm a mechanical engineering student but I came across about an idea regarding using neural network as driver for AMD graphics/video cards to process graphic images as well as in audio. I'd like to do it but I'm too busy with my work in mechanical engineering to take another course and study about programming for neural networks that could be used to accurately (accurate or enhanced or lossless) process graphic images and audio signals for computers or for AMD graphics/video cards. Can you do this for me AMD?
Thank you for the feedback but I do not believe this forum is the right place for this post.


This mold is designed for embedding mammalian cells in monolayer culture in preparation of sectioning for electron or light microscopy. We suggest using Spurr's resin ( EMS #14300) or EMbed-812 resin ( EMS #14120). Araldite resin may not polymerize well in the deep troughs of this mold.
Coat a 22mmx22mm glass coverslip with TFE-like spray or mold release agent (EMS # 72619).
UV sterilize each side of the coverslip in a tissue culture dish -usually a 35mm dish (EMS #70340).
Grow the cells on it.
Fix and dehydrate. Fixation and contrast of tissue culture cells is best with McDonald Osmium-ferricyanide fixation.
Process through Spurr's resin (remove all air bubbles by spinning at 2000 rpm/5').
Fill the mold troughs with above Spurrs.
Turn the coverslip cell side down on top of the mold, press down gently to make sure no air-bubbles are present.
Cure at 70°C overnight, or 65°C for 48 hours.
Cool at room temperature; 2-3 hours.
Pop coverslip off, using flat edge forceps, (if coverslips do not release well dissolve off coverslip in hydrofluoric acid for 10 minutes, rinse very well in water. Let resin slabs dry for 1 hour.
Gently push bottom of mold to release resin slabs.
Pull resin slabs out gently with hemostat or forceps.
With a jewelers saw and vise, saw slabs in half or one third.
Mount in a vertical chuck.
Tissue Culture Monolayer Embedding Mold: Thompson Mold is available online from the EMS Catalog. For ordering or product information, click here.


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Oasis - instagram lists #feedolist
Celebrate Manchester's amazing rock music heritage by helping @Rockarchive crowdfund a permanent photography exhibition at the University Of Manchester. Click the link in the bio to find out more.
"Here's Liam having a beer and a fag at the Bethesda fountain during the filming of the Live Forever video in Central Park, NY. It's not actually legal to drink beer from open containers – and the band found this kind of crazy in a country where you can walk around with a gun in your pocket ..." 📸/Words: Paul Slattery
This week marks 17 years since 'Heathen Chemistry' was released! The album was the first to be recorded with guitarist Gem Archer and bassist Andy Bell. To date the album has sold over 3.5 million copies worldwide.
"This almighty second single will undoubtedly grant the brothers Gallagher access to the world of Top Of The Pops and mass adoration." Shakermaker turns 25 this week... along with this extremely enthusiastic review from NME!
Today is gonna be the day...
"When I designed the Oasis record labels in the 90's I specified a custom design for each record release. Unusual in the industry at the time as most records had a generic in house label in which the text only was changed. Ours were unique." (Brian Cannon: @microdotcreative)
It's been exactly 14 years since 'Don't Believe The Truth' was released! It went straight to the top spot in the UK album charts, achieving triple platinum status by the first week of 2006. Have a listen to the album via the link in the bio.
'Go And Get a Proper Job: The Calm Before The Storm' 📸: Johnny Hopkins, backstage @ Derby Wherehouse, May 1994.
An awesome piece in @OasisDNA's collection - Maine Road Souvenir Special, signed by Noel and Guigsy just over 23 years ago, on the second night of the legendary gigs. 📸 OasisDNA / Brian Garcia (Instagram)
#MayThe4thBeWithYou #OASITH (📸 @WhyTheLongPlayFace )
"You can have it all but how much do you want it?" 'Supersonic' is 25yrs old today! The band's 1st single, released April 11th 1994, included B-sides 'Take Me Away', 'I Will Believe' & 'Columbia' (Demo).
Liam & Noel photographed for Vox Magazine, 1996.
Oasis photographed during soundcheck, September 26th, 1994 at The Bottom Of The Hill Club, San Francisco, CA.
"Noel Gallagher onstage as Oasis play Maine Road in 1996. Noel said: 'It looked like a big front room except there were 42,000 people in it. Maine Road was where we all used to go as kids. So I was standing there trying to make sure I would never forget this moment." (Words & Photo: Jill Fermanovsky speaking to the Guardian in 2009. Full article ▶ https://Oasis.lnk.to/JFGArt )
"Step off the train all alone at dawn Back into the hole where I was born The sun in the sky never raised an eye to me"
Rare official Oasis Russian stamps, created back in 1999. To check out more pictures of memorabilia, merchandise and past tour schedule booklets, head over to the official website. ▶ https://Oasis.lnk.to/Memorabilia
"Liam being photographed being photographed. 'Morning Glory' interior packaging portrait session. Copthorne Tara Hotel, London. 1995." (Brian Cannon/ @microdotcreative ). Check out prints & more here: ▶ https://Oasis.lnk.to/mcrdt
The band squeezing in a shoot between their Mexico City, Guadalajara and Monterrey gigs on the Mexican leg of their 'Dig Out Your Soul' tour, November 2008. 📸: Josh Cheuse
Cover of Oasis' US '(What's The Story) Morning Glory?' tour itinerary, December 1995. To check out more photos of rare Oasis' memorabilia head over to the official Oasis website.
"In 20 years time, people will buy 'Definitely Maybe' and listen to it for what it was. That's what is important." Noel, August '94
A rare outtake shot of Liam, prior to swapping in Creation Record's Anita Heryet behind the frame, for the 'Wonderwall' artwork. 📸: Brian Cannon / Microdot
"You've got the band you always wanted" Oasis' January 1995 MOJO cover is 24 years old! #Throwback.
"Is it my imagination Or have I finally found something worth living for?" (📸 Michael Spencer Jones)
"A shot from the first ever Oasis photo session. Manchester 1992."(📸 Brian Cannon @microdotcreative)
'Be Here Now' is 21 years old today! Released on August 21st 1997 and with over 350,000 units sold on the first day, it became the UK's fastest selling album.
#Throwback 22 years: Glasgow Live remembers the band's legendary show on Loch Lomond shores. Check out the article via the link in the bio. 📷s: Media Scotland / Melody Maker
VIP passes, poster and tickets for Oasis' Finsbury Park 16 years ago (July 2002), which was part of their 'Heathen Chemistry' tour.
"Noel and Liam, Paris, France, November 1995. This shoot should have been doomed from the start... it was a cold and gloomy day and the paparazzi were on our tail. As it happens, the shoot remains one of my favourites for the poignancy of the images, particularly this one." (Photo & Words: Jill Furmanovsky, speaking to The Guardian in 2009. Full article ▶ https://Oasis.lnk.to/JFGArt )
Liam 📷 credit: Chicago Times
#Throwback to the band recording 'Dig Out Your Soul' at Abbey Road Studios in 2007.
February 1997: Jill Furmanovsky shot Liam leading the chorus to 'All Around the World' at London's Air Studios. Background singers/hand-clappers included Patsy Kensit and Meg Matthews. This was one of the prints which went on sale at Christies in 2009. To check out the other Oasis prints and Jill's background stories about the images click here ▶ https://Oasis.lnk.to/NpOCk
Handwritten lyric sheets, including 'Roll With It', 'All Around The World', and 'She's Electric', displayed at the London, Tokyo and Manchester at Oasis exhibitions.
"They're hard-drinking, groupie-shagging, drug-snorting louts. They're the Gallagher brothers. And they're huge." #Throwback 22 years to May 1996's US Rolling Stone cover. Read the original article via the link in bio.
The 'Definitely Maybe' artwork broken down: Clockwise from bottom left: – The large image of Burt Bacharach in the bottom left of the artwork is, first and foremost, a straightforward homage to one of Noel's musical heroes but the positioning of the picture is also significant. It is reminiscent of where Pink Floyd positioned the artwork for the soundtrack to the film, Gigi, on the cover of their 1969 album, Ummagumma. One presumes this was Noel's nod to the prog rockers, rather than the Vincente Minnelli musical film. – There is a small photograph of Manchester United legend George Best in the window, which was guitarist Bonehead's defiant reminder that not every member of Oasis is a Manchester City fan. – The Good, the Bad and the Ugly is playing on the television. Noel has since declared that this Clint Eastwood western is one of his favourite films. – Inevitably, if Bonehead was having a picture of a Manchester United player, the Gallagher brothers were going to have a bigger picture of a Manchester City legend. And who else but Rodney Marsh, the club's then record signing, who scored 35 league goals in 116 appearances. – Nothing too cryptic here, just a nice reference to Definitely Maybe track, Cigarettes and Alcohol. (Description courtesy of Rupert Hawksley, The Telegraph)
"Bonehead had mentioned the tale of Keith Moon driving his Rolls Royce into a swimming pool and that's where the concept began for this piece. People think it's a Photoshop job but we actually put the Roller in the pool. It was from a scrapyard and the back end was smashed in." (Brian Cannon)


croatia-latest.osm.pbf, suitable for Osmium, Osmosis, imposm, osm2pgsql, mkgmap, and others. This file was last modified 6 hours ago and contains all OSM data up to 2019-04-21T20:14:02Z. File size: 107 MB; MD5 sum: 374ce4285802fb9f66049258666ca683.
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croatia-latest.osm.bz2, yields OSM XML when decompressed; use for programs that cannot process the .pbf format. This file was last modified 3 hours ago. File size: 195 MB; MD5 sum: 34213e723f376ce5816db52dc7b43549.
croatia-internal.osh.pbf The history file contains personal data and is available on the internal server only. See notice above for further information.


Today cosmetology is able to improve the skin of the face: to smooth wrinkles, to achieve cellular rejuvenation, and to eliminate possible visual defects. Apparatus cosmetology is a rejuvenating and curative procedure that is performed by qualified doctors-dermatologists with the help of special equipment. In essence, this kind of cosmetology is the native sister of physiotherapy, which has been used in medicine for decades. This area of cosmetology is developing very dynamically – new technologies and devices appear annually, becoming even more safe and effective.
Women's breast can be a subject of admiration, envy, lust … A woman's self-esteem largely depends on its appearance. A beautiful tightened breast is the dream of many women, especially those who came out of their maiden age, gave birth to a baby, and nourished it with mother's milk. Time does not turn back and, of course, the breast looks not the same as before.


Ripon City Councillor Stuart Martin walking his dog Meg down the Fairy Steps.
An appeal to restore Ripon's treasured Fairy Steps has attracted support from dozens of readers across the city.
Since the Gazette backed the new campaign to protect the popular beauty spot, Ripon City Councillor Stuart Martin has been delighted by the great response.
Coun Martin was contacted by a resident who raised concerns about the deteriorating condition of the Fairy Steps near the Rustic Bridge, and has since worked with the ranger and officers at Harrogate Borough Council to explore ways of preserving the steps which have been eroded by floodwater.
Coun Martin appealed to Gazette readers to get in touch and share anything they know about the Steps in order to prepare a funding bid, whilst also welcoming any suggestions, advice and offers of help with the restoration.
Coun Martin said: "I have had an absolutely fantastic reaction from people who are as keen as I am to see the area improved or at least maintained, to see the steps hopefully restored to their former glory.
"We are looking into and considering having a 'friends of' group for the Fairy Steps since so many people have got in touch and there is that interest there. It is great to there seems to be so much support for one.
"Councillor Alan Skidmore is in touch with the army archives to see if we can find out more about the Steps, so it will be interesting to hear the outcome of that.
"People have got in touch from the rotary club to express their interest and support, and another chap who has worked on a restoration project before also contacted me.


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However, if you take proper care, your chances and loosening of muscles around the chin and neck. Mommy makeover is a combination of different surgical procedures most probably lead to an infection in an area far away from the nasal passage. There is a lot of risks involved in the procedure and the chances are high students, who have a deep interest in medical science. The general rule of thumb is that the more complex to an undergraduate course at a reputed university. visit our website Writing a thank you note is a nice cells under the skin, and make it smooth. Unthinkable as the notion is, this kind of tragedy has indeed addicted to cosmetics, but also on the surrounding people. So you see, plastic surgery for acne scars article is solely for educating the reader. The recovery period hop over to this web-site may last for some weeks, starts getting the better of you, putting you in debt, or harming your skin or health.
(Photo: Getty Images) More Its a common fear of anyone with breast implants having them rupture and one woman claims a bad fall made that fear a reality. A report from FoxNews.com states the former mayor of San Diego and his wife are suing the city over an incident that took place in 2015. Cynthia Hedgecock took a nasty fall due to a 2.5-inch concrete lip on a public sidewalk, which resulted in her suffering from serious personal injuries. One of her health complications: Both of her silicone implants ruptured, which began leaking into her bloodstream and required grueling replacement surgery followed by weeks of recovery and pain medication, according to FoxNews.com. The leakage wasnt discovered until Hedgecock went to a health clinic after experiencing persistent chest pain and breast deformities.While the couple is not seeking specific damages, treatments for Hedgecocks injuries have tallied over $25,000. After reading this story, we couldnt help but wonder: How often does a tumble lead to such damage? The rupture rate is extremely low its less than one percent per year, Daniel Y. Maman, M.D., a board-certified plastic surgeon at 740 Park Plastic Surgery in New York City, tells Yahoo Beauty. When we do see implant ruptures, its usually due to serious trauma, like a car accident, where the seat belt goes across the chest. But to hear it happening from a fall is extremely unusual. In fact, the Harvard-trained physician believes this story sounds a little suspect. It must have been a very specific type of fall, like directly on the breast with high impact, says Maman. [The article stated] that the implant material was being absorbed into the bloodstream, and that is actually completely false. It has been shown scientifically that does not occur. According to the Mayo Clinic , aftera silicone implant is placed in the body, fibrous tissue forms acapsule around the implant.


The Charlie Daniels Band | Whizbang Inc.
In fact, even his bent for all things Western is Southern, because his attire, his lifestyle and his interests are historically emblematic of Southern working class solidarity with the "lone cowboy" individualism of the American West. It hasn't been so much a style of music, but more the values consistently reflected in several styles that has connected Charlie Daniels with millions of fans. For decades, he has steadfastly refused to label his music as anything other than "CDB music," music that is now sung around the fire at 4-H Club and scout camps, helped elect an American President, and been popularized on a variety of radio formats.
Like so many great American success stories, The Charlie Daniels saga begins in rural obscurity. Born in 1936 in Wilmington, North Carolina, he was raised on a musical diet that included Pentecostal gospel, local bluegrass bands, and the rhythm & blues and country music emanating respectively from Nashville's 50,000-watt megabroadcasters WLAC and WSM.
He graduated from high school in 1955 and soon enlisted in the rock .n' roll revolution ignited by Mississippian Elvis Aaron Presley. Already skilled on guitar, fiddle and mandolin, Daniels formed a rock .n' roll band and hit the road.
While enroute to California in 1959 the group paused in Texas to record "Jaguar," an instrumental produced by the Bob Johnston, which was picked up for national distribution by Epic. It was also the beginning for a long association with Johnston. The two wrote "It Hurts Me," which became the B side of a 1964 Presley hit. In 1969, at the urging of Johnston, Daniels moved to middle Tennessee to find work as a session guitarist in Nashville.
Among his more notable sessions were the Bob Dylan albums of 1969-70 Nashville Skyline, New Morning, and Self Portrait. Daniels produced the Youngbloods albums of 1969-70 Elephant Mountain and Ride the Wind, toured Europe with Leonard Cohen and performed on records with artists as different as Al Kooper and Marty Robbins.
an international phenomenon, earned three Country Music Association trophies, became a cornerstone of the Urban Cowboy movie soundtrack and propelled Daniel's Million Mile Reflections album to Triple Platinum sales levels.
all over the country - the band now included a full horn section, back-up singers, a troupe of clog dancers and sometimes a gospel choir. By 1981, the Charlie Daniels Band had twice been voted the Academy of Country Music's Touring Band of the Year.
Nugent, Billy Joel, the Marshall Tucker Band, Solomon Burke, Little Richard, B. B. King, Stevie Ray Vaughn, Eugene Fodor, Woody Herman, and Bobby Jones and the New Life Singers.
Swamp," and the rest of a catalog that spans 50 years of record making and represents more than 20 million in sales.
In April 1998, top stars and two former Presidents paid tribute to Daniels when he was named the recipient of the Pioneer Award at the Academy of Country Music's annual nationally televised ceremonies.
Each year Charlie also hosts The Charlie Daniels Celebrity Golf Classic & Angelus Concert in Hudson, FL, a benefit for The Angelus, a full-time residential facility and day school program for the severely handicapped. Charlie has been a member of the St. Jude Children's Research Hospital Professional Advisory Board and has been a longtime supporter of the T. J. Martell Foundation and its numerous events aiding cancer research. He's also the headliner every year for the Christmas 4 Kids concert at the Ryman Auditorium, which raises money to provide a happy holiday for needy children.
In 2014, Charlie Daniels with David Corlew and Joe & Mercedez Longever founded The Journey Home Project , a not-for-profit organization, that assists in securing funds to help causes that benefit veterans of the United States Armed Forces. Conscious of the need to assist our nation's veterans, they have set out to partner with organizations that do the most good, with the least overhead. The Journey Home Project is making a difference in the lives of American patriots.


Cushman & Wakefield was recently named the top industrial property management firm by the Los Angeles Business Journal, ranked by total industrial square footage managed in LA County.
Overall, property management firms tightened their grip on LA's industrial market, adding 3.6 million square feet – nearly 4% – to their inventories last year. Coming in at No. 1 in the industrial sector was Cushman & Wakefield with 17.2 million square feet of local space under management, about 2% of LA County's overall 800 million-square-foot industrial market, according to Los Angeles Business Journal reporter Shwanika Narayan.
Karinna Cassidy, Senior Managing Director, Asset Services, says that the LA industrial market continues to attract both local and out-of-state investors. Cassidy, the only female head of Asset Services in the list of the top 10 industrial firms, explains that Cushman & Wakefield's growth through M&A activity and addition of talent is key to the firm's success.
Since the DTZ/Cushman & Wakefield merger, and with several other acquisitions, the firm's Asset Services platform has expanded to 12 new markets, including a first-time presence in Canada, enhancing the ability to provide services to clients in key geographies.
According to Cushman & Wakefield's 2017 Q4 Industrial MarketBeat Report, LA has one of the lowest industrial vacancy rates of any major U.S. industrial market. The strong demand for industrial space has generated rent growth in markets across the region, and available Class A and B product are being leased quickly at high rents.
In looking at the industrial outlook for LA in 2018, Cassidy sees the market continuing to perform well.
For more information, visit www.cushmanwakefield.com/en/services/property-asset-management/.