5 weeks of adventure travel and volunteering in Thailand – the ultimate summer gap experience.
Sun, green hills and crystal clear waters.
Asian adventures – Water sports, trekking, paragliding and more!
You can travel for as long as you like, whenever you like but our next group program departs for 5 weeks in Thailand on 4 July 2019. You can stay for anywhere between 2-16 weeks depending on your plans. Check out our Gap Year programs if you are looking to stay for longer. We also offer group discounts if you wish to travel with your friends.
I participated in an Africa Asia Ventures trip in the summer and it was honestly one of the most impactful and incredible things I have done so far in my life!
The AV team was so helpful and kind, ensuring we were safe and were fully organised for the trip, and once out there the representatives were friendly and so easy to get on with. I didn't know anyone prior to the trip, but made a few good friends whilst there. The best thing about AV is you see first hand where your money goes, and how it directly benefits people. I was pushed out of my comfort zone in the best way possible, and in the process I learned so much about myself.
I could not recommend this programme more enthusiastically; I encourage all to take a look at it and consider going on the adventure of a life time!
Departs: Create your own customized trip, or join a scheduled group departure – the choice is yours.
Welcome and briefing – get to know your group and learn about your project and local life.
Your skills will be well used. Help with school renovation projects, in the classroom or other school activities.
Use your weekends to explore the local area, and take part in all that it has to offer.
Your flight will need to take you all the way through to Chiang Rai via Bangkok, arrive by midday on the day your summer program starts in Thailand and you will be met by an Africa & Asia Venture representative. After leaving Chiang Rai, you will travel for a couple of hours to the Maekok River Village Resort in Tha Ton, for an introduction to Thailand and a three-day briefing on your project.
After your briefing you will travel to your new home; a rural school not far from Tha Ton – your base for the next five weeks. You will spend this time living and working in the community, teaching English and getting involved with other activities in the community.
The Thai schools' English syllabus is entirely an exercise in writing, so neither teachers nor children get a chance to speak it. That's where you come in! You will teach each class in your school two or three times per week, helping the Thai English teacher with pronunciation and giving the children a massive boost in confidence. You may be asked by teachers and villagers to run separate classes for them. You can also play sports with the children (each school has a pitch at its centre) or perhaps help with art and paint some murals, with your pupils, to brighten up school buildings.
Each day you will spend time socialising with the villagers; teachers will explain the Thai way of life and traditions and, perhaps, teach you some Thai dancing or Takraw (a sort of cross between volleyball and football). This is a great chance to make new friends – they will be keen to talk about where you are from, your family and life at home, whilst you can practice your Thai!
The Maekok River Village Project have set up a Special Educational Needs Centre in Tha Ton, and Africa & Asia Venture volunteers are welcome to support the new facility and its students. Get in touch if you'd like to learn more about the Centre.
In your free time you can do as you wish and explore the area. The Maekok Resort will arrange for you to enjoy some weekend activities like trekking in Fang National Park, cycling, archery, rock climbing, Chiang Mai, and kayaking on the Maekok River!
After your final night in Chiang Rai we will take you to the airport in time to catch your flight home.
Your house will be situated on the school grounds, or nearby, giving you the chance to experience rural Thai life. An important part of the Africa & Asia Venture experience is living locally, shop at the markets, and help where you can.
The house itself will probably have a bedroom(s), a communal/cooking area and somewhere to wash. Electricity is intermittent at best, and your house will probably have a typical Asian loo. Wherever you will be staying, you will grow to love it and soon feel very much at home!
We encourage independence you will be doing your own washing, buying your food in nearby markets and prepare meals, a food allowance is provided by us. Lunch is normally cooked by the school. This is the best way of getting to know people, being involved in the community and gaining an understanding of living there – you are not a bystander watching from afar.
You will need to boil water from a local source or buy bottled water.
Create your own customized trip, or join a scheduled group departure – the choice is yours.
See our Group Program Dates & Costs for full details or contact us if you're looking for a customized trip.
You could try our Thailand gap year program.
Read reviews from Africa & Asia Venture gap year and summer volunteers.


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Toy Review: Masters of the Universe Origins Prince Adam Sky Sled
Luke Y. Thompson Saturday, September 05, 2020
The thinking behind the Masters of the Universe Origins line, currently exclusive to Walmart but soon to be available everywhere else, appears to be thus. Funko had some success making retro-figures in the vintage Masters of the Universe style, from horror icons to DC superheroes. At some point, Mattel executives had to notice, and realize they owned outright the toys being imitated. Hence Origins, which are essentially the original '80s figures with more articulation. But while in the '80s, the muscular sculpts looked to little kids like McFarlane-level detail compared to Star Wars figures, times change.
So Origins aren't for everyone: adult collectors tend to prefer the more detailed Classics line, or Mondo's $200+ 12-inchers. But for older fans who kept their vintage toys, these new ones are totally compatible. And for kids who play rough, they're simple and durable. But can vehicles like the Sky Sled work for Classics too?
Time to find out. The Sky Sled makes for a good basic vehicle to start out for a new line, as it's small enough not to price too high. It can also work as an "army builder" inspiring fans to buy multiples, especially with the allegiance-changing feature this one includes. Pop off the green side panels and griffin head to remove the good-guy features, and replace with red sides and a snake head to make it an evil Sky Sled.
But who can ride it? The design favors Origins figures with their vintage style and foot holes to attach to the pegs on the Sled's pedals. However, vintage figures fit perfectly too, like Skeletor in these photos. Classics come just a touch larger, and can ride if they are allowed to sit down and wrap their legs around the vehicle, like Eldor here. The controls are pretty thick, so a figure like Eldor with painted hands shouldn't be forced to hold them, but other classics with wider hands, perhaps.
RELATED: Toy Review: Gremlins Ultimate Flasher Figure by NECA
Whichever figure rides, the included, detachable stand can find a position to bear the weight. The vehicle's other action feature (as such) is that the front cannon swivels side to side, and features removable blast effect.
Most of the earliest Masters of the Universe vehicles, frankly, were just amorphous blobs for figures to sit on, given all their personality by the included stickers. The Sky Sled's sculpting is much more on a par with classics, giving the vehicle its character and design. The stickers on the side-panels and controls are pre-applied, so less hassle than vintage, though not as cool as the color printing on the classics version.
Prince Adam, however, misses a mark or two. The original vintage figure had an elastic waistband to hold the sword of power on his back. This one does not, thereby missing out on a key element of the character. The sword also has a much thicker handle than vintage, in keeping with the Sled's wider grips. This feels unnecessary, and makes it more difficult to hold. Plus it's pink — that's a nod to the original figure, of course, but to be accurate to virtually any tie-in media it ought to be gray. Either that or make it pink in the tie-in media. The inconsistency used to drive some '80s kids crazy.
RELATED: Toy Review: RAW10 Makes McFarlane Toys Monsters for Kids
Walmart's price hovers around $30, which is less than what the price of a Classics figure plus shipping used to be. For those who like the new Origins line, it's a good deal. But even for folks who want to stick with Classics, it's an affordable display piece. So long as one isn't a purist about the characters sitting down or crouching.
What type of Masters of the Universe collector are you? Check out our full gallery below, and let us know in comments.
Recommended Purchase: Masters Of the Universe Classics 2015, Laser Power He-man and Skeletor 2pack Adult collector
Tags: Masters of the Universe, Mattel

redpajama_set_name: RedPajamaCommonCrawl


Some of my favourite summer memories are of the big cook outs my Dad used to have for the neighbourhood; albeit living in the country the neighbours were really anyone within a few kilometer radius. My father is famous in these parts for his chicken rub which has clad not only your average bird, but on a few occasions, a turkey or two. Those hens would be lined up on a local farmer's outdoor rotisserie unit, customized to fit about 20 birds. Most of us couldn't resist but to check in periodically to see how close they were to being done, in anticipation of the crispy salty bites of skin, fresh and hot off the coals. The flavour of the outdoor rotisserie chicken in combination with his secret spice was something to not only taste, but dream about.
Luckily our family did not have to always wait for a big get-together, our small rotisserie barbecue was just the right size for two small chickens. We were one of those families that barbecued year-round, dusting off the snow when required, and using it almost daily throughout the summer. And when it came to cooking, my Mom could take it or leave it, so it was usually my Dad and I 'manning' the grill. I soon learned that it was important to bring the grill heat up to a high temperature to sear the meat, and that closing the lid helped to retain that heat. I was also instructed to never salt steaks too early as it can dry out the meat and, of course, to let it 'rest' before slicing and serving.
Over the years I have come up with my own preferences for grilling and, of course, these always lead to heated, but friendly 'debates' with my father. Each year, celebrating my small claim to fame of having two children born on the same date two years apart, at my son and daughter's annual birthday cook out, we'd have an annual sausage debate! I have been ordering mine with onion from the butcher for the past 15 years, yet he still tries to convince me that his original recipe with garlic is better. This question is usually followed by the discussion of whether or not his Broil King or my Weber reigns supreme!
Summer grilling brings together food, wine, family, friends and if you are looking for inspiration on what wines to enjoy this season look no further than your backyard. The Wineries of Niagara-on-the-Lake bring it all together with the SIP AND SIZZLE touring pass to kick off the season. They are firing up the heat this May to grill up some tasty treats matched with their favourite barbecue wines. So get your grilling on by visiting wineriesofniagaraonthelake.com for information and tickets.
Niagara Flavours by Andrea Kaiser.


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This Matt Every story involving a front-nine 29 and a helicoptered putter is the most Matt Every story ever
By Christopher Powers
Steph Chambers
Before making his television debut at the RSM Classic last month, Matt Every didn't rule out a return to professional golf should his competitive juices return. Clearly, they did this past weekend, as Every teed it up in a Florida Professional Golf Tour Event and did some very Matt Every things.
In the opening round of the Riviera Open—not that Riviera, the Riviera in Ormond Beach, Fla.—the two-time Arnold Palmer Invitational winner shot a one-under 70, a chaotic round that featured six birdies and five bogeys. But the chaos didn't truly begin for Every until the following day, this past Sunday, which was the final round of the 36-hole event.
RELATED: Tiger Woods set to return, will play with son Charlie at PNC Championship
According to Ryan French, AKA Monday Q Info (aka @acaseofthegolf1), Every went out in six-under 29, thrusting himself into contention. Things took a turn for the worse early on the back nine, as Every bogeyed the 10th and 12th holes, stunting his momentum. Nick Biondi, a mini tour pro who was in Every's group that day, relayed the rest of the story to French, and it is a doozy:
Every's other playing partner that day, Ohio club pro Jake Scott, replied to French's tweet saying that Every was a couple of four-foot putts away from 27 on the front nine. As for the putter, according to Scott, it was brand new:
Every's Sunday 63 vaulted him 30 spots up the leader board, good enough for solo sixth, four shots back of winner Jeff Coor. Per Scott, Every finished the round eagle-birdie, the final putt a 15-footer with his wedge.
Luckily for us, this will not be the last we see of Every on the golf course. While he's hoping for more television work, Biondi says that Every told him he plans to use all of his remaining starts that he'll earn due to his past champion status, somewhere in the range of 8-to-12. Expect to see him in all of the alternate-field events and, of course, the Arnold Palmer Invitational, where two years ago he shot rounds of 65 and 83 for one of the most electric missed cuts you'll ever see. Typical stuff for Every.
RELATED: Ranking golf's top 10 comeback stories of 2021


IPG combines Current Marketing, Creation; Arby's lifts lid on sandwich-themed subscription box results; Elon Musk helps Sheryl Crow; Joe Biden apology video mocked.
New from PRWeek this morning: Interpublic Group has merged Current Marketing and Creation into a midsize agency called Current Global, effective April 1. Current founder and president Virginia Devlin and Creation global president George Coleman are serving as co-CEOs of the new shop. Get the details here.
Arby's has the meats…and subscription boxes. But do people actually want a monthly sandwich-themed one? The answer is yes. All 250 six-month subscriptions sold out in 55 minutes, with more than 3,700 people signing up for the waitlist on ArbysoftheMonth.com. Arby's CMO Jim Taylor gave us the inside story on the campaign's strategy, tactics, and results.
Tesla reports biggest sales drop in history. About 63,000 vehicles were delivered to customers in the first three months of 2019 — a 31% drop compared to the prior quarter. It was the first quarter-to-quarter drop in sales at Tesla in nearly two years and the single largest drop ever. (CNN) In other Tesla news, the government's contempt case against CEO Elon Musk is scheduled to be heard in court today. And here's an interesting exchange Musk had on Twitter with singer Sheryl Crow yesterday (who, by the way, loves her Tesla).
Joe Biden's apology video is being mocked. The former Vice President addressed recent accusations of inappropriate behavior by several women's in a video posted to his Twitter and Instagram accounts on Wednesday. Biden explained "social norms have begun to change" and said he has always "tried to make a human connection." He ended the video saying he will be much more mindful of personal space. Biden's "social norms" statement generated backlash on Twitter, with some finding it tone-deaf. Salon called the video the "weirdest campaign kickoff ever" for the likely Democratic frontrunner; and Samantha Bee released her own delightfully awkward message in response to Biden on Wednesday night's episode of "Full Frontal."
And then there were eight. That's how many contenders are left in PRWeek's "One Shining Slogan" Bracket. Voting is open for the four matchups that will determine who advances to the semifinal round.


The artist Paolo Staccioli presents his latest show in Siena, in partnership with Liquid art system.
Guerrieri, Cavalli e Centauri (Warriors, Horses and Centaurs) is a great show with about fifty sculptures by the artist. Artworks by Paolo Staccioli follow the history: they talk about time and our past. Warriors and horses are the main protagonists of battles memories and ancient stories.
The exhibition will take place in a very wonderful and important location, at the Magazzini del Sale, part of the ancient Palazzo Pubblico of Siena, where history never left the city, from February 9 to April 23.


Babun | A windows shell you will love!
Would you like to use a linux-like console on a Windows host without a lot of fuzz? Try out babun!
EULAlyzer® - Analyze license agreements for interesting words and phrases. Make sense of the nonsensical.
Zorin OS is a multi-functional operating system designed specifically for Windows users who want to have easy and smooth access to Linux. It is based on Ubuntu which is the most popular Linux operating system in the world.
BoxCryptor is a cryptographic virtual harddisk that encrypts all data on-the-fly using the AES-256 standard. Encrypted data is stored in an arbitrary directory of your choice, e.g. one that is synced to the cloud like your Dropbox folder.
SecretSync works together with the Dropbox service to securely sync your files. It does this using client-side encryption, which means that before your files are added to Dropbox, they are encrypted first, on your computer.
Within minutes, our free data visualization tool can help you create an interactive viz and embed it in your website or share it. Anyone can do it, it's that easy—and it's free.
This Windows application will display and log weather data obtained through a USB connection to the Oregon Scientific WMR100, WMR200 and RMS300 weather stations. You will find features here for a wide range of users, from novices, to experts. The extensive user manual describes program features in detail and includes informative discussions on many topics.


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Global-warming
How to use Global-warming in a sentence
To stop the meltdown we must slow global warming.
The effects of global warming on the earth are far reaching indeed.
Smog was cited as a major factor that contributes to global warming.
Ocean water quality is also affected by global warming.
A major cause of increased global warming is man-made greenhouse gases, particularly CO2, which is a by-product of fossil fuels.
Do think you there's anything we can do fix some of the problems already caused by global warming?
Global warming affects everyone on the planet, and global warming prevention is both a personal and global responsibility.
Not everyone knows about global warming.
Some environment and energy experts doubt that the method is compatible with a world worried about global warming.
Understanding the facts on global warming is essential to preventing it.
Natural causes have been contributing to global warming since before recorded history.
Encourage others in your community to take action to stop global warming and climate change.
Nesting suggests global warming pied flycatchers at Coedydd Aber are the latest to suggest that global warming is happening.
The water vapor emitted at altitude by burning fuel also drives global warming.
Chlorofluorocarbons contribute to pollution and global warming.
We are now accustomed to tracing such gradual effects in numerous guises, from acid rain to global warming to pesticide residues.
The largest source of greenhouse gas contributing to global warming is the burning of fossil fuels.
Solar power can even be used to help reduce global warming.
Other potential effects of global warming include more frequent hurricanes and higher rates of some diseases, such as malaria.
In some areas, permafrost is now emitting carbon, which could potentially accelerate the greenhouse effect and global warming.
Pollution created by cars and light trucks accounts for nearly one-third of American carbon emission, and emissions of carbon dioxide from airplanes is responsible for an additional 3.5 percent of global warming.
Nitrogen oxides trap 300 times more heat per volume than carbon dioxide, making fertilizer use in farming one of the leading causes of global warming.
Scientists have spent several decades studying global warming in an attempt to predict what changes will occur in the future and determine how man can slow or stop these changes from taking place.
Unfortunately, no one can directly stop the natural contributors to global warming.
By taking steps to reduce your carbon footprint, you can help prevent global warming by decreasing the environmentally devastating effects of man-made causes.
However, the most significant cause of global warming is increasing levels of greenhouse gases.
Water vapor, the most powerful and prevalent greenhouse gas, is increasing due to global warming.
While limiting the use of personal cars and trucks will not completely eliminate pollution, it reduces the overall quantity of emitted gases and helps slow global warming.
Knowing more about the facts on global warming will help you take the actions to reduce your impact on the environment.
With all the talk about climate change in recent years, many people are wondering about the consequences of global warming.
Many discussions about the consequences of global warming degenerate into apocalyptic warnings, but this purpose of this article is not to scare you.
In fact, one of the most important facets of the global warming issue is that all the consequences are interrelated and actually start to compound each other as the earth's temperature continues to rise.
Wildlife - The effect of global warming on wildlife has been and will continue to be catastrophic.
The non-profit group Natural Resources Defense Foundation, or NRDC, has a list of five things we can do to stop global warming.
By reducing your reliance on fossil fuels, you can reduce your contribution to global warming.
From temperature increases and droughts to weather changes and rising sea levels, global warming directly or indirectly affects every aspect of our climate.
Perhaps the most devastating effect of global warming and climate change is the destruction of natural ecosystems.
The term global warming describes the average increase in the planet's temperature.
The Environmental Protection Agency offers a list of ways to help reduce greenhouse gas emissions and stop global warming at home, school, work, and on the road.
When the topic of global warming comes up it's clear that there is still some controversy about the subject, and people ask if it is humans causing global warming or if it's a natural planetary cycle.
While that much is true, many people get a little vague when it comes to the greenhouse effect and how it's connected to global warming.
If the original question is, "are humans causing global warming?" then the next logical question would be to ask if there are ways to stop global warming.
Humans might not be able to stop global warming completely, but greenhouse gases can certainly be reduced in both big and little ways.
Debating whether global warming is human caused or a natural planetary cycle is almost a moot point when decreasing the greenhouse effect and slowing global warming is humanly possible.
Perhaps more than any other environmental issue, the true facts about global warming have created their own controversy.
Online news sites carry daily stories about whether or not global warming exists.
The term global warming creates some of the confusion.
The first step in trying to understand global warming is to recognize the complexity of the issue.
However, global warming makes people realize how interdependent the nations of the world are even if they are not aware of all the essential cycles and processes going on in the natural world.
Effects of global warming are not the same everywhere.
The true facts of global warming were not determined by one study.
One study doesn't necessarily mean global warming isn't occurring.
Since the greenhouse effect is a natural and beneficial phenomenon, some people don't believe that human actions have contributed to global warming and climate change.
Nuclear energy does not produce carbon dioxide, sulfur dioxide or any other gasses that some believe contribute to global warming.
In this way, wind energy benefits the environment and reduces the emissions many believe are responsible for climate change or global warming.
Since the majority of electricity used is generated by fossil fuels, using it causes the atmosphere becomes polluted with carbon dioxide (CO2), one of the substances many believe is linked to global warming.
There are many causes and effects of global warming.
One of the biggest causes of global warming is the huge amount of carbon dioxide emissions released into the atmosphere each day.
Manufacturing, production, industry and development are the best examples of what's termed as global warming contributors.
Newer science studies and experiment have shifted mainstream terminology and many global warming supporters are revising their opinions on CO2 effects, replacing these with natural solar activity and cosmic rays.
The goal of the carbon exchange is to universally lower the amount of carbon emissions being produced, which will in turn lower the amounts of air pollution and the threat of global warming.
Besides her musical endeavors, Sheryl Crow is also a political activist focusing her energy on global warming and international relations.
Tila Tequila just keeps melting down as though she's experiencing her own personal global warming.
The concern over global warming and pollution has moved the auto industry into producing vehicles that are more fuel-efficient and less taxing on the environment.
Modern believers in the prophesy point to the current debate about climate change and global warming as proof positive that the world is indeed heading for the kinds of changes that the Mayans predicted.
A study of global warming might be another good idea.
Finally, come up with new kids science projects that supplement the current materials, whether it is a tornado experiment for kids or a project that goes along with a global warming for kids unit.
In addition, kids will certainly benefit from the study and understanding of global warming.
He wrote and starred in a 1995 movie based on the song and has also adapted the lyrics for Climate Mash, a song about global warming.
One of the consequences of global warming in mountain regions is increasing risk of infectious diseases.
Between them, belching and biomass burning make the second largest contribution to global warming after fossil fuel burning.
Understanding the climate system is a major research challenge given urgency by global warming.
Has he not read the previous debate on spiked - commissioned by his own organization - where the global warming case took a drubbing?
It is believed Mr Blair will outline key future policies aimed at curbing the emission of the greenhouse gases that cause global warming.
Let me briefly describe our action plan to combat global warming here in Los Angeles.
These costs will increase if the U.S. does nothing to curb global warming.
Carbon neutral Our way of life, transport, production and consumption all need to change if we want to halt global warming.
Carbon emissions are the key problem causing global warming.
For example turning off lights that are not being used helps to reduce global warming.
Many climatologists believe that the decline in mountain glaciers is one of the first observable signs of human-induced global warming.
With man-made global warming out of the equation, mankind's ' consumption ' in biosphere terms is entirely sustainable.
There is growing evidence that current global warming is human-induced and that it is having widespread impacts.
It is this accelerated melting which is considered by many scientists to be evidence of greater global warming brought on by humans.
The most likely potential source for such an anomaly is meltwater from the antarctic ice sheet in a global warming scenario.
In the context of global warming there are a wealth of targets for action including legislators, corporate executives and the general public.
Anyone who is concerned by the threat of Global Warming is invited to join the marchers.
This water war follows several disastrous monsoons in India - the probable result, campaigners argue, of global warming.
This research establishes for the first time a direct link between global warming and the soot particles emitted by motor vehicles.
Its cousins now proliferate in many northern gardens, their owners made hopeful by global warming.
Plus, you've got the complication of global warming driving fish stocks north.
Understanding how climate change affects mountains is vital as governments and international organizations develop strategies to reverse current global warming trends.
You can forget about Global Warming and start digging out those thermal undies for year-round wear, and ice-fishing may become the new craze.
Questioning the wisdom He is not alone in doubting the scientific veracity of global warming claims.
There was, he said, no scientific evidence of global warming.
Plus, you 've got the complication of global warming driving fish stocks north.
Last month it congratulated itself on observing no activity among Global Warming scaremongers during the big freeze, thereby grossly underestimating human ingenuity.
Some people see the increase in temperature as an indictment of global warming.
One of the proposed alternative energy sources for decreasing the effects of global warming (climate change) is solar power.
Many scientists and environmentalists feel that there must be a significant decrease in greenhouse gas emissions in order to decrease global warming.
The question of whether or not solar energy should be included in the plan to fight global warming was posed as a debate on Juggle.
Arguments on both sides of the issue bring up valid points both for and against the use of solar power to combat global warming.
Solar power should be used as one of many weapons in an arsenal to combat global warming.
Discussing the effects of global warming on the earth can easily take up an entire book, but there are a few basics that demonstrate how changes in the earth's atmosphere are dramatically affecting life on this planet.
The effects of global warming on the earth also affect human lives, as seen from the impact of increased natural disasters such as hurricanes.
The World Heath Organization stated that global warming could cause a dramatic increase in insect-borne diseases in places like Great Britain and Europe.
These examples demonstrate that global warming affects every living thing on the planet.
These scientists think that global warming may lead to more extreme weather such as dry spells, intense rain, hurricanes and increased heat, which results in glacier melting and an increase in sea levels.
These are renewable energy sources and provide clean energy, which does not contribute to global warming.
Many sources exploring the subject never take the time to definitively give meaning to the term global warming.
According to the United States Environmental Protection Agency, global warming refers to climate change that causes an increase in the average temperature of the lower atmosphere of the Earth.
First and foremost, just be aware of the activities you participate in that increase greenhouse gases, which in turn increases the effects of global warming.
In order to reduce the effects of global warming in the future, it's going to take a group effort.
Carbon cycle activity gives us a general idea of the carbon stores and exchanges between the biosphere, geosphere, hydrosphere and atmosphere of the earth, which gives us insight into what humans are doing to cause global warming.
When you read that global warming can only really be combated if government and industry step up, it's easy to become discouraged; but fortunately, there are many simple ways to save the environment on an individual basis.
Other homeowners, not willing to make a complete investment, are still using solar power to decrease global warming by purchasing products such as solar power roof vents and attic fans to defer some of their energy footprint.
While the Earth's climate has changed many times during its history, there appears to be a rapid global warming occurring based on many human factors.
Higher temperatures from global warming cause ice and glaciers to melt and water to expand which results in the rise in water levels.
Carbon monoxide is poisonous to humans and animals, and carbon dioxide is a primary greenhouse gas, contributing to global warming.
Even the most hardcore global warming skeptic is usually impressed by a few basic energy conservation facts, simply because a lot of tips for saving energy are also cost effective, both on the personal and business levels.
This was an opportunity to change American energy habits significantly, and global warming might not be nearly so large a threat had the political will and financial wherewithal been in place.
It's fair to wonder how we can recycle to save our planet, since it seems like such a small thing to do in the face of so much else that needs to be done to reduce the threat of global warming.
The more tons of greenhouse gases we all emit, the more we are speeding up the pace of global warming.
There is solid evidence that emissions from fossil fuels are contributing to many issues facing the planet, including global warming and pollution, and these types of organic fuel sources offer some solutions.
By purchasing a wind power kit for your home, you can greatly reduce or even eliminate your monthly power bill while knowing that you are using power that does not contribute to global warming.
The hope with this kind of technology is that the more that it is implemented around the world, the more it can be a benefit to help reduce global warming.
Most man-made causes of global warming result from an increase in greenhouse gases, which are gases that trap or absorb infrared radiation emitted from the planet.
Not all causes of global warming are man-made.
The five things are to set limits on global warming pollution, invest in more green jobs and energy, drive cars that get better gas mileage, build green homes and buildings, and build better transportation networks.
Make sure you understand what you can do to stop global warming and then try to live are green as possible, and encourage as many people as you can to do the same.
Climate change brought on by global warming is one of the greatest threats the planet is currently facing.
A primary cause of global warming and climate change is an increase is greenhouse gas emissions.
How does global warming affect our climate?
Increasing demand for types of renewable energy will help reverse global warming and reduce our reliance on fossil fuels.
By learning how to stop global warming and making changes in your daily life, you can reduce your carbon footprint and help the environment.
Meat and dairy contribute to greenhouse gas emissions and global warming.
Increase awareness of global warming in your community by living green and encouraging others to do the same.
ThinkQuest provides comprehensive information on global warming and its effects on the planet in an easy to understand format.
The National Geographic website contains an abundance of information on many global warming topics, including deforestation, biofuels, toxic waste, and air pollution.
Burning fossil fuels increases greenhouse gas emissions and contributes to global warming.
Most people have the notion that global warming means that the average temperature of the earth is on the rise.
The consequences of global warming are just too important to ignore.
While global warming and other environmental issues are politically hot topics, you should learn the basics before you choose your position.
By promoting renewable energy, the Midwest would be able to reduce its carbon footprint drastically and the pollution that causes global warming.
While strides have been made curbing pollutants, such as sulfur dioxide, carbon dioxide gas emissions remain the greatest threat in terms of its potential to aid global warming.
While researchers are learning more and more, there is still much about global warming and its effect on the environment that no one understands.
Climate change is a catch-all term that replaced global warming and includes more than human activity that many scientists claim to be responsible for greenhouse gases.
On this track from his new album, he gives up the name crusade for a more timely crusade - global warming awareness.
She collaborated with Laurie David on an anti global warming campaign and has been active in fundraisers for scleroderma and cancer.
The net contribution of dryland degradation to global warming is difficult to quantify at this time.
In conclusion, perhaps the scale of global warming has been overstated by omitting to take into account fossil fuel depletion.
It's time Esso woke up to the horror of climate change, and stopped fue l ling the fires of global warming.
Or digging out scientists to explain that global warming is a pile of alarmist dog pooh.
If the case against global warming skeptics is so strong, why is it necessary to indulge in ad hominem attacks and smears?
Even the most strident global-warming deniers have taken to saying that a little warming is a good thing.
We have a 12-point plan on how to tackle global warming in the UK.
Global Warming vs. Climate Change: How Are They Different?
Do you know the difference between global warming and climate change? While these two words are similar, they have distinct definitions. Learn what global warming vs. climate change means. Explore examples of what climate change and global warming look like.
Examples of Global Warming: Impact and Possibilities
Global warming refers to climate change as a result of greenhouse gases. Experts believe that if people don't make an effort to stop air pollution and the release of greenhouse gases, climate change resulting from global warming will change the world. Discover many examples of global warming that have already occurred, as well as others that are predicted to happen in the future.
Words near global-warming in the Dictionary
global system for mobile communication
global system for mobile communications
global teacher
global unicast address
global-warming-denial
global-weirding
globar
globard
globate


It's conflating two trademarks so that people who own neither can profit. And again, if that isn't copyright violation, I don't know what is.
Trademarks and copyrights are different things. This is a copyright case and a copyright ruling.
Copyrights protect an expression of an idea, but not an idea (or a style) itself. You cannot copyright the drawing or prose style of a Dr. Seuss book.
You cannot copyright a character in a sci-fi franchise. You can copyright a specific illustration, you can copyright a specific textual work, you can copyright a screenplay or a television show.
Combining ideas from two other sources and blending them together with your own ideas can result in a new creative work that is copyrightable. Let's not overlook that this book does include a new story, new prose, and new illustrations that, while they have features in common with some previous works, are indeed new and novel.
Let's not forget that this kind of mashup was a longtime staple in Hollywood. "High concept" boiled down to mixing key ideas from popular movies or shows in order to create something new. Hell, Star Trek creator Roddenberry pitched the original series as "Wagon Train to the stars." He didn't need to get permission from NBC because he borrowed the idea of a group of characters journeying into a frontier from their popular western series.
In a legal sense, a trademark is a recognizable branding elements that consumers are supposed to be able to rely on to know the origin of a product. You could have a trademark in an image of Kirk or Spock or the Enterprise, and put it on the packaging so that your customers know this is the real deal, official licensed toys. The Cat in the Hat logo is probably trademarked by the Dr. Seuss estate, and only printed on their official publications. The key to a trademark suit is that it has to be identical or similar enough as to confuse potential customers as to the source of the goods it's on. I doubt that there is a trademark claim to be made here because the Seuss Estate or Paramount (or CBS or whoever the hell owns the Star Trek rights now) would have filed a trademark case in addition to or instead of this copyright suit.
In a conventional sense, a trademark can be something more general, like a style. You can say there's a trademark style to Dr. Seuss prose and imagery, but that would have no bearing on a copyright case, nor is it likely to be relevant in a trademark lawsuit.
Oft times Shakespeare appropriated works only a few years old; Othello (1603) was based off Un Capitano Moro (1565), less than forty years old, still in copyright in most copyright systems. If you want to discuss principle, not law, then don't dismiss that as "old folk tales"; wrestle with the fact that Shakespeare's works could not be written with your principles.
Copyright law does work like this, at least in the Southern District of California; the judge said so. Wishing may not make it so, but if this decision is overturned, then we may in a democracy petition our representatives to make it so. Stop bringing law up as a shield if you're talking about principle; in law, in theory we the people can do pretty much whatever we want.
How are they doing something at someone else's expense? How is this costing Dr Seuss Enterprises? It's not high art, but these type of works can be amusing, and it doesn't hurt anyone else. Star Trek might have more of a complaint; copyright law often turns on careful study of the works, so I'd find it hard to say without reading the book.
These types of questions are much harder than you're giving them credit for. One artist does something, and many others follow the same lead, sometimes in clearly fair ways, sometimes rather closely. The Monkees were a ripoff of the Beatles, as were many other bands. D&D and Shannara cribbed quite a few things from Tolkien. Without Anne Rice, there probably wouldn't be Twilight. Without Sherlock Holmes, probably no Poirot.


text:
Q: How to add Windows explorer favorites on user logon I have some Favorites links in Windows explorer (not IE). I am looking for a way to apply those favorites to administrator@mycompany.com account each time I log on to any PC in our company.
I have a login.vbs script based on PC name, not user name.
What is the best way to do this for admin account? I dont find too much options for favorites in windows explorer.

redpajama_set_name: RedPajamaStackExchange


text:
Alan Lambowitz
Professor, Professor of Oncology
Molecular Biosciences, Department of Oncology
Mr. and Mrs. A. Frank Smith, Jr. Regents Chair in Molecular Biology
lambowitz@austin.utexas.edu
Brooklyn College, Department of Chemistry, B.S., 1968, Summa cum laude with honors in Chemistry; Yale University, Department of Molecular Biophysics and Biochemistry, M. Phil., 1970, Ph.D., 1972 (Ph.D. Thesis in laboratory of Prof. Carolyn Slayman); Postdoctoral Fellow, University of Pennsylvania, Johnson Research Foundation, Department of Biophysics and Physical Biochemistry (Prof. Walter Bonner), 1972-1973; Research Associate, Rockefeller University, Laboratory of Cell Biology (Prof. David Luck), 1973-1975; Senior Staff Fellow, National Institute of Mental Health, Laboratory of General and Comparative Biochemistry, 1975-1976; The Edward A. Doisy Department of Biochemistry, Saint Louis University School of Medicine, Assistant Professor, 1976-1979; Associate Professor, 1979-1982; Professor 1982-1986; Medical Mycology Center, Washington University School Medicine, Barnes and Wohl Hospitals, (Dr. G. Medoff, Chief), 1978 to 1986; Ohio Eminent Scholar in Molecular Genetics, Professor of Molecular Genetics, Biochemistry, and Medical Biochemistry, The Ohio State University, Colleges of Biological Sciences and Medicine, 1986-1997. The Ohio State University Biotechnology Center, 1987-1997; Director, Institute for Cellular and Molecular Biology, 1997-2016. Mr. and Mrs. A. Frank Smith, Jr. Regents Chair in Molecular Biology and Professor of Molecular Biosciences, University of Texas at Austin, September 1997-present. Professor of Oncology, Dell Medical School, 2019-present.
NIH Molecular Biology Study Section, 1982-1986; Neurospora Policy Committee, 1982-1986; Ohio Science and Engineering Round Table, 1990-1997; ASM Council, 1991-1993; The Academy of Medicine, Engineering, and Science of Texas, Board of Directors, 2010-2012; Scientific Advisory Board, RNA Institute of the University at Albany-State University of New York, 2012-present; Editor, Plasmid, 1987–1998; Editorial Board, Molecular and Cellular Biology (ASM), 1988-2009; Associate Editor, Fungal Genetics and Biology, 1988-2005; Editorial Board, RNA, 1997-present; Editorial Board, Proceedings of the National Academy of Sciences, 2006-2009. Editorial Board, Mobile DNA, 2014-present.
Research Summary:
The research in our laboratory focuses on group II intron and related bacterial and organellar reverse transcriptases (RTs), their reverse transcription mechanisms, association with CRISPR-Cas systems, and applications in high-throughput RNA sequencing (RNA-seq), including for the analysis of human cellular, exosomal, and plasma RNAs for RNA diagnostics and liquid biopsy of human diseases. We use a combination of genetic, biochemical, microbiological, and structural approaches. Methods employed include X-ray crystallography and cryo-EM, as well as high-throughput RNA and DNA sequencing and a wide range of bioinformatic and computational methods for analyzing sequence data and biomarker identification in clinical samples, including in currently funded clinical trials.
2020 Yao J, Wu DC, Nottingham RM, Lambowitz AM. Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling. eLife.
2019 Lentzsch AM, Yao J, Russell R, Lambowitz AM. Template switching mechanism of a group II intron-encoded reverse transcriptase and its implications for biological function and RNA-Seq. Journal of Biological Chemistry.
2019 Temoche-Diaz MM, Shurtleff MJ, Nottingham .MR, Yao J, Fadadu RP, Lambowitz AM, Schekman R. Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes. eLife, 8.
2019 Reinsborough CW, Ipas H, Abell NS, Nottingham RM, Yao J, Devanathan SK, Shelton SB, Lambowitz AM, Xhemalce B. BCDIN3D regulates tRNAHis 3' fragment processing. PLoS Genetics, 15(7).
2019 Belfort M, Lambowitz AM. Group II Intron RNPs and ReverseTranscriptases: From Retroelements to Research Tools. Cold Spring Harbor Perspective in Biology.
2019 Xu H, Yao J, Wu DC, Lambowitz AM. Improved TGIRT-seq methods for comprehensive transcriptome profiling with decreased adapter dimer-formation and bias correction. Scientific Reports, 9(1).
2018 Mohr G, Silas S, Stamos J, Makarova KS, Markham LM, Yao J, Lucas-Elio P, Sanchez-Amat A, Fire AZ, Koonin EV, et al. A Reverse Transcriptase-Cas1 Fusion Protein Contains a Cas6 Domain Required for Both CRISPR RNA Biogenesis and RNA Spacer Acquisition. Mol. Cell, (72):700-714.
2018 Boivin V, Deschamps-Francoeur G, Couture S, Nottingham RM, Bouchard-Bourelle P, Lambowitz AM. Simultaneous sequencing of coding and noncoding RNA reveals a human transcriptome dominated by a small number of highly expressed noncoding genes. RNA, 24(7):950-965.
2018 Mohr G, Kang SY, Park SK, Qin Y, Grohman J, Yao J, Stamos JL, Lambowitz AM. A Highly Proliferative Group IIC Intron from Geobacillus stearothermophilus Reveals New Features of Group II Intron Mobility and Splicing. Journal of Molecular Biology, 430(17):2760-2783.
2018 Wu DC, Yao J, Ho KS, Lambowitz AM, Wilke CO. Limitations of alignment-free tools in total RNA-seq quantification. BMC Genomics, 19(1):510.
2017 Stamos J, Lentzsch AM, Lambowitz AM. Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications. Molecular Cell, 68:926–939.
2017 Carrell ST, Tang Z, Mohr S, Lambowitz AM, Thorton CA. Detection of expanded RNA repeats using thermostable group II intron reverse transcriptase. Nucleic Acids Research.
2017 Shurtleff MJ, Yao J, Qin Y, Nottingham RM, Temoche-Diaz M, Schekman R, Lambowitz AM. Broad role for YBX1 in defining the small noncoding RNA composition of exosomes. National Academy of Sciences, 114(43).
2017 Silas S, Makarova KS, Shmakov S, Páez-Espino D, Mohr G, Liu Y, Davidson M, Roux S, Krishnamurthy SR, Fu B, et al. On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires. American Society for Microbiology, 8(4).
2017 Wu DC, Lambowitz AM. Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching. Scientific Reports, 7(8421).
2017 Zubradt M, Gupta P, Persad S, Lambowitz AM, Weissman JS, Rouskin S. DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo. Nat Methods, 14(1):75-82.
2016 Burke JM, Kincaid RP, Nottingham RM, Lambowitz AM, Sullivan CS. DUSP11 activity on triphosphorylated transcripts promotes Argonaute association with noncanonical viral microRNAs and regulates steady-state levels of cellular noncoding RNAs. Genes Dev, 30(18):2076-2092.
2016 Lamech LT, Saoji M, Paukstelis PJ, Lambowitz AM. Structural Divergence of the Group I Intron Binding Surface in Fungal Mitochondrial Tyrosyl-tRNA Synthetases That Function in RNA Splicing. J Biol Chem, 291(22):11911-27.
2016 Nottingham RM, Wu DC, Qin Y, Yao J, Hunicke-Smith S, Lambowitz AM. RNA-seq of human reference RNA samples using a thermostable group II intron reverse transcriptase. RNA, 22(4):597-613.
2016 Qin Y, Yao J, Wu DC, Nottingham RM, Mohr S, Hunicke-Smith S, Lambowitz AM. High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases. RNA, 22(1):111-28.
2016 Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, Lambowitz AM, Fire AZ. Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein. Science, 351 (6276):aad4234.
2015 Qin Y, Yao J, Wu DC, Nottingham RM, Mohr S, Hunicke-Smith S, Lambowitz AM. High-throughput sequencing of human plasma RNA by using thermostable group II intron reverse transcriptases. RNA, Jan;22(1):111-28.
2015 Zheng G, Qin Y, Clark WC, Dai Q, Yi C, He C, Lambowitz AM, Pan T. Efficient and quantitative high-throughput tRNA sequencing. Nat Methods, Sep;12(9):835-7.
2015 Lambowitz, A.M. and Belfort, M. Mobile bacterial group II introns at the crux of eukaryotic evolution. Microbiol. Spectrum, 3(1) MDNA3-0050-2014. LINK
2015 Truong DM, Hewitt CF, Hanson JH, Cui X, Lambowitz AM. Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation. PLoS Genet, Aug 4;11(8):e1005422.
2015 Shen, P.S., Park, J., Qin, Y., Li, X., Parsawar, K., Larson, M., Cox, J., Cheng, Y., Lambowitz, A.M., Weissman, J.S., Brandman, O., and Frost A. Rqc2p and the 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains, Science, 347, 75-78. LINK
2014 Lamech, L., Mallam, A.L., and Lambowitz, A.M. Evolution of RNA-protein interactions: non-specific binding led to RNA splicing activity of fungal mitochondrial tyrosyl-tRNA synthetases. PLoS Biol., 12, e1002028. LINK
2014 Mallam, A.L., Sidote, D.J., and Lambowitz, A.M. Molecular insights into RNA and DNA helicase evolution from the determinants of specificity for a DEAD-box RNA helicase, eLife;3:e04630. LINK
2014 Katibah, G.E., Qin, Y., Sidote, D.J., Yao, J., Lambowitz, A.M.*, and Collins, K*. Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5., Proc. Natl. Acad. Sci. USA 111, 12025-12030, *Co-corresponding authors. LINK
2014 Hong, W., Zhang, J., Feng, Y., Mohr, G., Lambowitz, A.M., Cui, G.-Z., Liu, Y., and Cui, Q. The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons., Biotechnology for Biofuels, 7:80. LINK
2014 Enyeart, P.J., Mohr, G., Ellington, A.D., and Lambowitz A.M., Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis., Mobile DNA 5:2. LINK
2013 Enyeart, P.J., Chirieleison, S.M., Dao, M.N., Perutka, J., Quandt, E.M., Yao, J., Whitt, J.T., Keatinge-Clay, A.T., Lambowitz, A.M., and Ellington, A.D., Generalized bacterial genome editing using mobile group II introns and Cre-lox., Mol. Systems Biol. 9, 685. LINK
2013 Truong, D., Sidote, D., Russell, R., and Lambowitz, A.M., Enhanced group II intron retrohoming in magensium-deficient Escherichia coli via selection of mutations in the ribozyme core., Proc. Natl. Acad. Sci. USA 110, E3800-E3809. LINK
2013 Saldanha, R.J., Pemberton, A., Shiflett, P., Perutka, J., Whitt, J.T., Ellington, A., Lambowitz, A.M., Kramer, R., Taylor, D., and Lamkin, T.J., Rapid targeted gene disruption in Bacillus anthracis., BMC Biotechnology 13, 72. LINK
2013 Mohr, S., Ghanem, E., Smith, W., Sheeter, D., Qin, Y., King, O., Polioudakis, D., Iyer, V.R., Hunicke-Smith, S. Swamy, S., Kuersten, S., and Lambowitz, A.M., Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation sequencing. , RNA 19: 958-970. LINK
2013 Mohr, G., Hong, W., Zhang, J., Cui, G-z., Yang, Y., Qiu, C., Liu, Y-j., Lambowitz, A.M., A targetron system for gene targeting in thermophiles and its application in Clostridium thermocellum. , PLoS ONE 8(7): e69032. LINK
2013 Russell, R., Jarmoskaite, I., and Lambowitz, A.M., Toward a molecular understanding of RNA remodeling by DEAD-box proteins, RNA Biology 10: 43-54. LINK
2013 Yao, J., Truong, D.M., and Lambowitz, A.M., Genetic and biochemical assays reveal a key role for replication restart proteins in group II intron retrohoming., PLOS Genetics 9: e1003469. LINK
2012 Mallam A.L., Del Campo M., Gilman B., Sidote D.J., and Lambowitz A.M. , Structural basis for RNA-duplex recognition and unwinding by the DEAD-box helicase Mss116p., Nature 490: 121-125. LINK
2012 White, T.B., and Lambowitz, A.M., The retrohoming of linear group II intron RNAs in Drosophilia melanogaster occurs by both DNA ligase 4-dependent-and-independent mechanisms., PLoS Genetics 8: e1002534. LINK
2011 Lambowitz AM, Zimmerly S., Group II introns: mobile ribozymes that invade DNA., The RNA Worlds, 4th Edition (R.V. Gesteland, T.R. Cech, and J.F. Atkins, Editors), Cold Spring Harbor Press Perspect. Biol. 3(8): a003616. LINK
2011 Mohr, G., Del Campo, M., Turner, K.G., Gilman, B., Wolf, R.Z., and Lambowitz, A.M., High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p., J. Mol. Biol. 413: 952-972. LINK
2011 Mallam, A.L., Jarmoskaite, I., Tijerina, P., Del Campo, M., Seifert, S., Guo, L., Russell, R., and Lambowitz, A.M., Solution structures of DEAD-box RNA chaperones reveal conformational changes and nucleic acid tethering by a basic tail., Proc. Natl. Acad. Sci., USA 108: 12254-12259. LINK
2011 Portatz, J.P., Del Campo, M., Wolf, R., Lambowitz, A.M., and Russell, R., ATP-dependent roles of the DEAD-box protein Mss116p in group II intron splicing in vitro and in vivo., Mol. Biol. 411: 661-679. LINK
2011 Paukstelis, P.J., Chari, N., Lambowitz, A.M., and Hoffman, D., NMR strucure of the C-terminal domain of a tyrosyl-tRNA synthetase that funtions in group I intron splicing., Biochemistry 50: 3816-3826. LINK
2010 Gu, S.-Q., Cui, X., Mou, S., Mohr, S. Yao, J., and Lambowitz A.M., Genetic identification of potential RNA-binding regions in a group II intron-encoded reverse transcriptase., RNA 16: 732-747. LINK
2010 Lambowitz, A.M. and Zimmerly, S., Group II introns: mobile ribozymes that invade DNA. In: The RNA World, 4th Edition (R.F. Gesteland, T.R. Cech, and J.F. Atkins, Editors), Cold Spring Harbor Perspect Biol 1: a003616. LINK
2010 Mohr, G., Ghanem, E., Lambowitz, A.M., Mechanisms used for genomic proliferation by thermophilic group II introns., PLoS Biology 8: e1000391. LINK
2010 Gu, S.-Q., Cui, X., Mou, S., Mohr, S., Yao, J., and Lambowitz, A.M., Genetic identification of potential RNA-binding regions in a group II intron-encoded reverse transcriptase., RNA 16: 732-747
2009 Zhuang, F., Mastroianni, M., White, T.B., and Lambowitz, A.M., Linear group II intron RNAs can retrohome in eukaryotes and may use non-homologus end-joining for cDNA ligation., Proc. Natl. Acad. Sci., USA 106: 18189-18194
2009 Del Campo and Lambowitz, A.M., Structure of the yeast DEAD-box protein Mss116p reveals two wedges that crimp RNA., Mol. Cell 35: 598-609
2009 Del Campo and Lambowitz, A.M., Crystallization and preliminary X-ray diffraction of the DEAD-box protein Mss116p complexed with an RNA oligonucleotide and AMP-PNP., Acta Crystallographica Section F, Structural Biology and Crystallization Communications 65: 832-835
2009 Markov, D.A., Savkina, M., Anikin, M., Del Campo, M. Ecker, K., Lambowitz, A.M., DeGnore, J.P., McAllister, W.T. , Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purfication., Yeast 26: 423-440.
2009 Del Campo, M., Mohr, S., Jiang, Y., Jia, H., Jankowsky, E., and Lambowitz, A.M., Unwinding by local strand separation is critical for the function of DEAD-box proteins as RNA chaperones., J Mol Biol. 389: 674-693
2009 Zhuang, F., Karberg, M., Petrutka, J., and Lambowitz, A.M., EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting., RNA 15: 432-449
2008 Chen, Y., Potratz, J.P., Tijerina, P., Del Campo, M., Lambowitz, A.M., and Russell, R., DEAD-box proteins can completely separate an RNA duplex using a single ATP., Proc Natl Acad Sci U S A. 105: 20203-20208
2008 Vicens Q, Paukstelis PJ, Westhof E, Lambowitz AM, Cech TR, Towards predicting self-splicing and protein-facilitated splicing of group I introns., RNA 14: 2013-2029
2008 Paukstelis, P.J., Lambowitz, A.M., Identification and evolution of fungal mitochondrial tyrosyl-tRNA synthetases with group I intron splicing activity., Proc. Natl. Acad. Sci. USA 105: 6010-6015
2008 Dai, L., Chai, D., Gu, S.Q., Gabel, J., Noskov, S.Y., Blocker, F.J., Lambowitz, A.M., Zimmerly, S., A three-dimensional model of a group II intron RNA and its interaction with the intron-encoded reverse transcriptase., Mol. Cell 30: 472-485
2008 Zhao, J., Niu, W., Yao, J., Mohr, S., Marcotte, E.M., Lambowitz, A.M., Group II intron protein localiztion and insertion sites are affected by polyphosphate., PLoS Biol. 6: e150
2008 Mastroianni, M., Watanabe, K., White, T.B., Zhuang, F., Vernon, J., Matsuura, M., Wallingford, J., Lambowitz, A.M., Group II intron-based gene targeting reactions in eukaryotes., PLoS ONE 3: e3121
2008 Paukstelis, P.J., Chen, J.H., Chase, E., Lambowitz, A.M., Golden, B.L., Structure of a tyrosyl-tRNA synthetase splicing factor bound to a group I intron RNA., Nature 451: 94-97
2007 Del Campo, M., Tijerina, P., Bhaskaran, H., Mohr, S., Yang, Q., Jankowsk, E., Russell R., and Lambowitz, A.M., Do DEAD-Box proteins promote group II intron splicing without unwinding RNA?, Mol. Cell 28: 159-166
2007 Yang, Q., Del Campo, M., Lambowitz, A.M., and Jankowsky, E., DEAD-box proteins unwind duplexes by local strand separation., Mol. Cell 28: 253-263
2007 Yao, J., and Lambowitz, A.M., Gene targeting in Gram-negative bacteria by use of a mobile group II intron ("targetron") expressed from a broad-host-range vector., Appl. Environ. Microbiol. 73: 2735-2743
2007 Grohman, J.K., Del Campo, M., Tijerina, P., Lambowitz, A.M., and Russell, R., Probing the mechanisms of DEAD-box proteins as general RNA chaperones: the C-terminal domain of CYT-19 mediates general recognition of RNA., RNA 46: 3013-3022
2006 Halls, C., Mohr, S., Del Campo, M., Yang, Q., Jankowsky, E., and Lambowitz, A.M., Involvement of DEAD-box proteins in group I and group II intron splicing. Biochemical characterization of Mss116p, ATP hydrolysis-dependent and -independent mechanisms, and general RNA chaperone activity., J. Mol. Biol. 365: 835-855
2006 Noah, J.W., Park, S., Whitt, J.T., Perutka, J., Frey, W., and Lambowitz, A.M., Atomic force microscopy reveals DNA bending during group II intron ribonucleoprotein particle integration into double-stranded DNA., Biochem. 45: 12424-12435
2006 Yao, J., Zhong, J., Fang, Y., Geisinger, E., Novick, R.P., and Lambowitz, A.M., Use of targetrons to disrupt essential and non-essential genes in Staphylococcus aureus reveals temperature-sensitivity of Ll.LtrB group II intron splicing., RNA 12: 1271-1281
2006 Pyle, A.M., and Lambowitz, A.M., Group II introns: ribozymes that splice RNA and invade DNA. In: The RNA World, 3rd edition., 469-505
2006 Mohr, S., Matsuura, M., Perlman, P.S., and Lambowitz, A.M., A DEAD-box protein by itself promotes group II intron splicing and reverse splicing by acting as an RNA chaperone., Proc. Natl. Acad. Sci. USA 103: 3569-3574
2006 Yao, J., Fang, Y., Geisinger, E., Novick, R.P., and Lambowitz, A.M., Use of targetrons to disrupt essential and non-essential genes in Staphylococcus aureus reveals temperature-sensitivity of Ll.LtrB group II intron splicing., RNA 12: 1271-1281
2005 Zhao, J., and Lambowitz, A.M., A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles., Proc. Natl. Acad. Sci. USA 102: 16233-16140
2005 Smith, D., Zhong, J., Matsuura, M., Lambowitz, A.M., and Belfort, M., Recruitment of host functions suggests a repair pathway for late steps in group II intron retrohoming., Genes & Devel. 19: 2477-2487
2005 Yao, J., Zhong, J., and Lambowitz, A.M., Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library., Nucl. Acids Res. 33: 3351-3362
2005 Jones, J.P., III, Kierlin, M.N., Coon, R.G., Perutka, J., Lambowitz, A.M., and Sullenger, B.A., Retargeting mobile group II introns to repair mutant genes., Mol. Therapy 11: 687-694
2005 Coros, C.J., Landthaler, M., Piazza, C.L., Beauregard, A., Esposito, D., Perutka, J., Lambowitz, A.M., and Belfort, M., Retrotransposition strategies of the Lactococcus lactis Ll.LtrB group II intron are dictated by host identity and cellular environment., Mol. Microbio. 56: 509-524
2005 Lambowitz, A.M., Mohr, G., and Zimmerly, S., Group II intron homing endonucleases: ribonucleoprotein complexes with programmable target specificity. In: Nucleic Acids and Molecular Biology., 16: 121-145
2005 Paukstelis, P., Coon, R., Madabusi, L., Nowakowski, J., Monzingo, A., Robertus, J., and Lambowitz, A.M., A tyrosyl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Mol. Cell 17: 417-428
2005 Blocker, F.J.H., Mohr, G., Conlan, L.H., Li, Q., Belfort, M., and Lambowitz, A.M., Domain structure and three-dimensional model of a group II intron-encoded reverse transcriptase., RNA 11: 14-28
2005 Huang, H.-R., Rowe, C.E., Mohr, S., Jiang, Y., Lambowitz, A.M., and Perlman, P.S., The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function., Proc. Natl. Acad. Sci. USA 102: 163-168
2005 Paukstelis, P., Coon, R., Madabusi, L, Nowakowski, J. Monzingo, A., Robertus, J. and Lambowitz, A.M., A tyrosl-tRNA synthetase adapted to function in group I intron splicing by acquiring a new RNA binding surface., Mol. Cell 17: 417-428
2004 Watanabe, K., and Lambowitz, A.M., High-affinity binding site for a group II intron-encoded reverse transcriptase/maturase within a stem-loop structure in the intron RNA., RNA 10: 1433-1444
2004 Lambowitz, A.M., and Zimmerly, S., Mobile group II introns., Annu. Rev. Genet. 38: 1-35
2004 Cui, X., Matsuura, M., Wang, Q., Ma., H., and Lambowitz, A.M., A group II intron-encoded maturase functions preferentially in cis and requires both the reverse transcriptase and X domains to promote RNA splicing., J. Mol. Biol. 340: 211-231
2004 Chen, X., Mohr, G., and Lambowitz, A.M., The Neurospora crassa CYT-18 protein C-terminal RNA-binding domain helps stabilize interdomain tertiary interactions in group I introns., RNA 10: 634-644
2004 Lambowitz, A.M. and Zimmerly, S., Mobile group II introns., Annu. Rev. Genet. 38: 1-35
2004 Perutka, J., Wang, W., Goerlitz, D. and Lambowitz, A.M., Use of computer-designed group II introns to disrupt Escherichia coli DExH/D-box protein and DNA helicase genes., J. Mol. Biol. 336: 421-439
2003 Noah, J.W., and Lambowitz, A.M., Effects of maturase binding and Mg2+ concentration on group II intron RNA folding investigated by UV-cross-linking., Biochemistry 42: 12466-12480
2003 Huang, M.-R., Chao, M.Y., Armstrong, B., Wang, Y., Lambowitz, A.M., and Perlman, P.S., The DIVa maturase binding site in the yeast group II intron aI2 is essential for intron homing but not for in vivo splicing., Mol. Cell. Biol. 23: 8809-8819
2003 Zhong, J., and Lambowitz, A.M., Group II intron mobility using nascent strands at DNA replication forks to prime reverse transcription., EMBO J. 22 : 4555-4565
2003 Munoz-Adelantado, E., San Filippo, J., Mart�nez-Abarca, F., Garc�a-Rodr�guez, F.M., Lambowitz, A.M., and Toro, N., Mobility of the Sinorhizobium meliloti group II intron RmInt1 occurs by reverse splicing into DNA targets, but requires an unknown reverse transcriptase priming mechanism., J. Mol. Biol. 327 : 931-943
2003 Zhong, J., Karberg, M., and Lambowitz, A.M., Targeted and random bacterial gene disruption using a group II intron (targetron) vector containing a retrotransposition-activated selectable marker., Nucl. Acids Res. 31: 1656-1664
2003 Aizawa, Y., Xiang, Q., Lambowitz, A.M., and Pyle, A.M., The pathway for DNA recognition and RNA integration by a group II intron retrotransposon., Mol. Cell 11: 795-805
2003 Frazier, C.L., San Filippo, J., Lambowitz, A.M., and Mills, D.A., Genetic manipulation of Lactococcus lactis using targeted group II introns: generation of stable insertions without selection., Appl. Environ. Microbiol. 69: 1121-1128
2003 Mohr, G., and Lambowitz, A.M., Putative proteins related to group II intron reverse transcriptase/maturases are encoded by nuclear genes in higher plants., Nucl. Acids. Res. 31: 647-652
2002 San Filippo, J., and Lambowitz, A.M., Characterization of the C-terminal DNA-binding/DNA endonuclease region of a group II intron-encoded protein., J. Mol. Biol. 324: 933-951
2002 Mohr, S., Stryker, J., and Lambowitz, A.M., A DEAD-box protein functions as an ATP-dependent RNA chaperone in group I intron splicing., Cell 109: 769-779
2002 Singh, R.N., Saldanha, R.J., D'Souza, L.M., and Lambowitz, A.M., Binding of a group II intron-encoded reverse transcriptase/maturase to its high affinity intron RNA binding site involves sequence-specific recognition and autoregulates translation., J. Mol. Biol. 318: 287-303
2002 Myers, C.A., Kuhla, B., Cusack, S., and Lambowitz, A.M., tRNA-like recognition of group I introns by a tyrosyl-tRNA synthetase., Proc. Natl. Acad. Sci. USA 99 : 2630-2635
2002 Belfort, M., Derbyshire, V., Parker, M.M., Cousineau, B., and Lambowitz, A.M., Mobile introns: pathways and proteins. In: Mobile DNA II (N.L. Craig, R. Craigie, M. Gellert, and A.M. Lambowitz, eds.), 761-783
2001 Matsuura, M., Noah, J.W., and Lambowitz, A.M., Mechanism of maturase-promoted group II intron splicing., EMBO J. 20 : 7259-7270
2001 Karberg, M., Guo, H., Zhong, J., Coon, R., Perutka, J., and Lambowitz, A.M., Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria., Nature 19 : 1162-1167
2001 Dickson, L., Huang, H., Liu, L., Matsuura, M., Lambowitz, A.M. and Perlman, P.S., Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites., Proc. Natl. Acad. Sci. USA 98: 13207-13212
2001 Singh, N.N. and Lambowitz, A.M. , Interaction of a group II intron ribonucleoprotein endonuclease with its DNA target site investigated by DNA footprinting and modification interference., J. Mol. Biol. 309: 361-386
2001 Caprara, M.G., Myers, C.A., and Lambowitz, A.M., Interaction of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (CYT-18 protein) with the group I intron P4-P6 domain. Thermodynamic analysis and the role of metal ions., J. Mol. Biol. 309: 361-386
2001 Mohr, G., Rennard, R., Cherniack, A.D., Stryker, J., and Lambowitz, A.M., Function of the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase in RNA splicing. Role of the idiosyncratic N-terminal extension and different modes of interaction with different group I introns., J. Mol. Biol. 307: 75-92
2000 Eskes, R., Liu, L., Ma, H., Chao, M.Y., Dickson, L., Lambowitz, A.M. and Perlman, P.S., Multiple homing pathways used by yeast mitochondrial group II introns., Mol. Cell. Biol. 20: 8432-8446
2000 Chen, X., Gutell, R.R., and Lambowitz, A.M., Function of tyrosyl-tRNA synthetase in splicing group I introns. An induced fit model for binding to the P4-P6 domain based on analysis of mutations at the junction of the P4-P6 stacked helices., J. Mol. Biol. 301: 265-283
2000 Mohr, S., Wanner, L.A., Bertrand, H., and Lambowitz, A.M., Characterization of an unusual tRNA-like sequence found inserted in a Neurospora retroplasmid., Nucl. Acids Res. 28: 1514-1524
2000 Mohr, G., Smith, D., Belfort, M., and Lambowitz, A.M., Rules for DNA target-site recognition by a lactococcal group II intron enable retargeting of the intron to specific DNA sequences., Genes & Devel. 14: 559-573
2000 Guo, H., Karberg, M., Long, M., Jones, J.P. III, Sullenger, B., and Lambowitz A.M., Group II introns designed to insert into therapeutically-relevant DNA target sites in human cells., Science 289: 452-457
1999 Wank, H., San Filippo, J., Singh, R.N., Matsuura, M., and Lambowitz, A.M., A reverse transcriptase/maturase promotes splicing by binding at its own coding segment in a group II intron RNA., Mol. Cell 4: 239-250
1999 Saldanha, R., Chen, B., Wank, H., Matsuura, M., Edwards, J., and Lambowitz, A.M., RNA and protein catalysis in group II intron splicing and mobility reactions using purified components., Biochemistry 38: 9069-9083
1999 Zimmerly, S., Moran, J.V., Perlman, P.S., and Lambowitz, A.M. , Group II intron reverse transcriptase in yeast mitochondria. Stabilization and regulation of reverse transcriptase activity by the intron RNA., J. Mol. Biol. 289: 473-490
1999 Lambowitz, A.M., Caprara, M.G., Zimmerly, S., and Perlman, P.S., Group I and group II ribozymes as RNPs: clues to the past and guides to the future. In: The RNA World, Second Edition (R.F. Gesteland, T.R. Cech, and J.F. Atkins, Editors), 451-485
1998 Cousineau, B., Smith, D., Lawrence-Cavanagh, S., Mueller, J.E., Yang, J., Mills, D., Manias, D., Dunny, G., Lambowitz, A.M., and Belfort, M., Retrohoming of a bacterial group II intron: mobility via complete reverse splicing independent of homologous recombination., Cell 54: 451-462
1998 Yang, J., Mohr, G., Perlman, P.S., and Lambowitz, A.M., Group II intron mobility in yeast mitochondria: target DNA-primed reverse transcription activity of aI1 and reverse splicing into DNA transposition sites in vitro., J. Mol. Biol 282: 505-523
1997 Matsuura, M., Saldanha, R., Ma, Hongwen., Wank, H., Yang, J., Mohr, G., Cavanagh, S., Dunny, G.M., Belfort, M., and Lambowitz, A.M., A bacterial group II intron encoding reverse transcriptase, maturase, and DNA endonuclease activities: biochemical demonstration of maturase activity and insertion of new genetic information within the intron., Genes & Devel. 11: 2910-2924
1997 Guo, H., Zimmerly, S., Perlman, P.S., and Lambowitz, A.M., Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA., EMBO J. 16: 6835-6848
1997 Chen, B., and Lambowitz, A.M., De novo and DNA primer-mediated initiation of cDNA synthesis by the Mauriceville retroplasmid reverse transcriptase involve recognition of a 3' CCA sequence., J. Mol. Biol. 271: 311-332
1997 Chiang, C.-C., and Lambowitz, A.M., The Mauriceville retroplasmid reverse transcriptase initiates cDNA synthesis de novo at the 3' end of tRNAs.The Mauriceville retroplasmid reverse transcriptase initiates cDNA synthesis de novo at the 3' end of tRNAs., Mol. Cell. Biol. 17: 4526-4535
1997 Eskes, R., Yang, J., Lambowitz, A.M., and Perlman, P.S., Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing., Cell 88: 865-874
1997 Wallweber, G.J., Mohr, S., Rennard, R., Caprara, M.G., and Lambowitz, A.M., Characterization of Neurospora mitochondrial group I introns reveals different CYT-18 dependent and independent splicing strategies and an alternative 3' splice site for an intron ORF., RNA 3: 114-131
1996 Caprara, M.G., Lehnert, V., Lambowitz, A.M., and Westhof, E., A tyrosyl-tRNA synthetase recognizes a conserved tRNA-like structural motif in the group I intron catalytic core., Cell 87: 1135-1145
1996 Myers, C.A., Wallweber, G.J., Rennard, R., Kemel, Y., Caprara, M.G., Mohr, G., and Lambowitz, A.M., A tyrosyl-tRNA synthetase suppresses structural defects in the two major helical domains of the group I intron catalytic core., J. Mol. Biol. 262: 87-104
1996 Saldanha, R., Ellington, A., and Lambowitz, A.M., Analysis of the CYT-18 protein binding site at the junction of stacked helices in a group I intron RNA by quantitative binding assays and in vitro selection., J. Mol. Biol. 261: 23-42
1996 Yang, J., Zimmerly, S., Perlman, P.S., and Lambowitz, A.M., Efficient integration of an intron RNA into double-stranded DNA by reverse splicing., Nature 381: 332-335
1996 Caprara, M.G., Mohr, G., and Lambowitz, A.M., A tyrosyl-tRNA synthetase protein induces tertiary folding of the group I intron catalytic core., J. Mol. Biol. 257: 512-531
1996 Zimmerly, S., Guo, H., Eskes, R., Yang, J., Perlman, P.S., and Lambowitz, A.M., A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility., Cell 83: 529-538
1995 Zimmerly, S., Guo, H., Perlman, P.S., and Lambowitz, A.M., Group II intron mobility occurs by target DNA-primed reverse transcription., Cell 82: 545-554
1995 Moran, J.V., Zimmerly, S., Eskes, R., Kennell, J.C., Lambowitz, A.M., Butow, R.A., and Perlman, P.S., Mobile group II introns of yeast mitochondrial DNA are novel site-specific retroelements., Mol. Cell. Biol. 15: 2828-2838
1995 Kennell, J.C., Saville, B., Mohr, S., Kuiper, M., Sabourin, J.R., Collins, R.A., and Lambowitz, A.M., The VS catalytic RNA replicates by reverse transcription as a satellite of a retroplasmid., Genes & Devel. 9: 294-303
1995 Lambowitz, A.M., and Chiang, C.-C., The Mauriceville and Varkud plasmids: primitive retroelements found in Neurospora mitochondria. Proceedings Fifth International Congress of Mycology, Vancouver, B.C., Canada, August 1994, Can. J. Botany 73 (Suppl. 1): S173-S179
1995 Chiang, C.-C., Kennell, J.C., Wanner, L.A., and Lambowitz, A.M., A mitochondrial retroplasmid integrates into mtDNA by a novel mechanism involving the synthesis of a hybrid cDNA and homologous recombination., Mol. Cell. Biol. 14: 6419-6432
1995 Saldanha, R.J., Patel, S.S., Surendran, R., Lee, J.C., and Lambowitz, A.M, Involvement of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing. A new method for purifying the protein and characterization of physical and enzymatic properties pertinent to splicing., Biochemistry 34: 1275-1287
1994 Mohr, G., Caprara, M.G, Guo, Q., and Lambowitz, A.M., A tyrosyl-tRNA synthetase can function similarly to an RNA structure in the Tetrahymena ribozyme., Nature 370: 147-150
1994 Kennell, J.C., Wang, H. and Lambowitz, A.M. , The Mauriceville plasmid of Neurospora spp. uses novel mechanisms for initiating reverse transcription in vivo., Mol. Cell. Biol. 14: 3094-3107
1993 Wang, H., and Lambowitz, A.M., The Mauriceville plasmid reverse transcriptase can initiate cDNA synthesis de novo and may be related to reverse transcriptase and DNA polymerase progenitor., Cell 75: 1071-1081
1993 Mohr, G., Perlman, P.S., and Lambowitz, A.M., Evolutionary relationships among group II intron-encoded proteins and identification of a conserved domain that may be related to maturase function., Nucl. Acids Res. 21: 4991-4997
1993 Wang, H., and Lambowitz, A.M., Reverse transcription of the Mauriceville plasmid of Neurospora. Lack of RNase H activity associated with the reverse transcriptase and possible use of mitochondrial RNase H., J. Biol. Chem. 268: 18951-18959
1993 Lambowitz, A.M., and Belfort, M., Introns as mobile genetic elements., Annu. Rev. Biochem. 62: 587-622
1993 Kennell, J.C., Moran, J.V., Perlman, P.S., Butow, R.A., and Lambowitz, A.M., Reverse transcriptase activity associated with maturase-encoding group II introns in yeast mitochondria., Cell 73: 133-146
1993 Saldanha, R., Mohr, G., Belfort, M., and Lambowitz, A.M., Group I and group II introns., FASEB J. 7: 15-24
1992 Wang, H., Kennell, J.C., Kuiper, M.T.R., Sabourin, J.R., Saldanha, R., and Lambowitz, A.M., The Mauriceville plasmid of Neurospora crassa. Characterization of a novel reverse transcriptase that begins cDNA synthesis at the 3' end of template RNA., Mol. Cell. Biol. 12: 5131-5144
1992 Guo, Q., and Lambowitz, A.M., A tyrosyl-tRNA synthetase binds specifically to the group I intron catalytic core., Genes & Dev 6: 1357-1372
1992 Mohr, G., Zhang, A., Gianelos, J.A., Belfort, M., and Lambowitz, A.M., The Neurospora CYT-18 protein suppresses defects in the phage T4 td intron by stabilizing the catalytically active structure of the intron core., Cell 69: 483-494
1992 K�mper, U., K�ck, U., Cherniack, A.D., and Lambowitz, A.M., The mitochondrial tyrosyl-tRNA synthetase of Podospora anserina is a bifunctional enzyme active in protein synthesis and RNA splicing., Mol. Cell. Biol. 12: 499-511
1992 Nargang, F.E., Pande, S., Kennell, J.C., Akins, R.A., and Lambowitz, A.M., Evidence that a 1.6 kilobase region of Neurospora spp. mtDNA was derived by insertion of part of the LaBelle mitochondrial plasmid., Nucl. Acids Res. 20: 1101-1108
1992 Turcq, B., Dobinson, K.F., Serizawa, N., and Lambowitz, A.M., A protein required for RNA processing and splicing in Neurospora mitochondria is related to gene products involved in cell cycle protein phosphatase functions., Proc. Natl. Acad. Sci. USA 89: 1676-1680
1991 Mohr, G., and Lambowitz, A.M., Integration of a group I intron into a ribosomal RNA sequence promoted by a tyrosyl-tRNA synthetase., Nature 354: 164-167
1991 Kubelik, A.R., Turcq, B., and Lambowitz, A.M., The Neurospora crassa cyt-20 gene encodes cytosolic and mitochondrial valyl-tRNA synthetases and may have a second function in addition to protein synthesis., Mol. Cell. Biol. 11: 4022-4035
1991 Kittle, J.D., Jr., Mohr, G., Gianelos, J.A., Wang, H., and Lambowitz, A.M., The Neurospora mitochondrial tyrosyl-tRNA synthetase is sufficient for group I intron splicing in vitro and uses the carboxyl-terminal tRNA-binding domain along with other regions., Genes & Devel. 5: 1009-1021
1991 Schulte, U. and Lambowitz, A.M., The LaBelle mitochondrial plasmid of Neurospora intermedia encodes a novel DNA polymerase that may be derived from a reverse transcriptase., Mol. Cell. Biol. 11: 1696-1706
1991 Guo, Q., Akins, R.A., Garriga, G., and Lambowitz, A.M., Structural analysis of the Neurospora mitochondrial large rRNA intron and construction of a mini-intron that shows protein-dependent splicing., J. Biol. Chem. 266: 1809-1819
1990 Lambowitz, A.M., and Perlman, P.S., Involvement of aminoacyl-tRNA synthetases and other proteins in group I and group II intron splicing., TIBS 15: 440-444
1990 Akins, R.A., and Lambowitz, A.M., Analysis of large deletions in the Mauriceville and Varkud mitochondrial plasmids of Neurospora., Current Genetics 18: 365-369
1990 Cherniack, A.D., Garriga, G., Kittle, J.D., Akins, R.A., and Lambowitz, A.M., Function of Neurospora mitochondrial tyrosyl-tRNA synthetase in RNA splicing requires an idiosyncratic domain not found in other synthetases., Cell 62: 745-755
1990 Kuiper, M.T.R., Sabourin, J.R., and Lambowitz, A.M., Identification of the reverse transcriptase encoded by the Mauriceville and Varkud mitochondrial plasmids of Neurospora., J. Biol. Chem. 265: 6936-6943
1990 Kubelik, A.R., Kennell, J.C., Akins, R.A., and Lambowitz, A.M., Identification of Neurospora mitochondrial promoters and analysis of synthesis of the mitochondrial small rRNA in wild-type and the promoter mutant [poky]., J. Biol. Chem. 265: 4515-4526
1989 Drygas, M.E., Lambowitz, A.M., and Nargang, F.E., Cloning and analysis of the Neurospora crassa gene for cytochrome c heme lyase., J. Biol. Chem. 264: 17897-17906
1989 Dobinson, K.F., Henderson, M., Kelley, R.L., Collins, R.A., and Lambowitz, A.M., Mutations in nuclear gene cyt-4 of Neurospora crassa result in pleiotropic defects in processing and splicing of mitochondrial RNAs., Genetics 123: 97-108
1989 Kennell, J.C., and Lambowitz, A.M., Development of an in vitro transcription system for Neurospora mtDNA and identification of transcription initiation sites. , Mol. Cell. Biol. 9: 3603-3613
1989 Majumder, A.L., Akins, R.A., Wilkinson, J.G., Kelley, R.L., Snook, A.J., and Lambowitz, A.M., Involvement of tyrosyl-tRNA synthetase in splicing of group I introns in Neurospora crassa mitochondria. Biochemical and immunochemical analysis of splicing activity., Mol. Cell. Biol. 9: 2089-2104
1989 Lambowitz, A.M., Infectious introns (Minireview), Cell 56: 323-326
1989 Lambowitz, A.M., Sabourin, J.R., Bertrand, H., Nickels, R., and McIntosh, L., IImmunological identifcation of the alternative oxidase of Neurospora crassa mitochondria., Mol. Cell. Biol. 9: 1362-1364
1988 Akins, R.A., Kelley, R.L. and, Lambowitz, A.M., Characterization of mutant mitochondrial plasmids of Neurospora spp. that have incorporated tRNAs by reverse transcription., Mol. Cell. Biol. 9: 678-691
1988 Kuiper, M.T.R. and Lambowitz, A.M., A novel reverse transcriptase activity associated with mitochondrial plasmids of Neurospora., Cell 55: 693-704
1988 Akins, R.A., Grant, D.M., Stohl, L.L., Bottorff, D.A., Nargang, F.E. and Lambowitz, A.M., Nucleotide sequence of the Varkud mitochondrial plasmid of Neurospora and synthesis of a hybrid transcript with a 5' leader derived from mitochondrial RNA., J. Mol. Biol. 204: 1-25
1988 Mann, B.J., Akins, R.A., Lambowitz, A.M., and Metzenberg, R.L., The structural gene for a phosphorus-repressible phosphate permease in Neurospora crassa can complement a mutation in the positive regulatory gene nuc-1., Mol. Cell. Biol. 8: 1376-1379
1988 Kuiper, M.T.R., Holtrop, M., Venema, H., Lambowitz, A.M., and de Vries, H., A 3' splice site mutation in a nuclear gene encoding a mitochondrial ribosomal protein in Neurospora crassa., J. Biol. Chem. 263: 2848-2852
1988 Kuiper, M.T.R., Akins, R.A., Holtrop, M., de Vries, H. and Lambowitz, A.M., Isolation and analysis of the Neurospora crassa cyt-21 gene: A nuclear gene encoding a mitochondrial ribosomal protein., J. Biol. Chem. 263: 2840-2847
1987 Akins, R.A. and Lambowitz, A.M., A protein required for splicing group I introns in Neurospora mitochondria is mitochondrial tyrosyl-tRNA synthetase or a derivative thereof., Cell 50: 331-345
1987 Paietta, J.V., Akins, R.A., Lambowitz, A.M. and Marzluf, G.A., Molecular cloning of the cys-3+ regulatory gene of Neurospora crassa., Mol. Cell. Biol. 7: 2506-2511
1986 Lambowitz, A.M., Akins, R.A., Kelley, R.L., Pande, S. and, Nargang, F.E. , Mitochondrial plasmids of Neurospora and other filamentous fungi. In: Extrachromosomal Elements in Lower Eukaryotes (R.B. Wickner, A. Hinnebusch, A.M. Lambowitz, I.C. Gunsalus, and A. Hollaender, eds.), 83-92
1986 Medoff, G., Maresca, B., Lambowitz, A.M., Kobayashi, G., Painter, A., Sacco, M. and Carratu, L., A correlation between pathogenicity and temperature-sensitivity of Histoplasma capsulatum., J. Clin. Invest. 78: 1638-1647
1986 Akins, R.A., Kelley, R.L. and Lambowitz, A.M., Mitochondrial plasmids of Neurospora: Integration into mitochondrial DNA and evidence for reverse transcription in mitochondria., Cell 47: 505-516
1986 Garriga, G. and Lambowitz, A.M., Protein-dependent splicing of a group I intron in ribonucleoprotein particles and soluble fractions., Cell 46: 669-680
1986 Garriga, G., Lambowitz, A.M., Inoue, T. and Cech, T.R., Mechanism of recognition of the 5' splice site in self-splicing group I introns., Nature 352: 86-89
1986 Vincent, R.D., Goewert, R., Goldman, W.E., Kobayashi, G.S., Lambowitz, A.M., and Medoff, G., Classification of Histoplasma capsulatum isolates by restriction fragment polymorphisms., J. Bacteriol. 165: 813-818
1985 Lambowitz, A.M., Akins, R.A., Garriga, G., Henderson, M., Kubelik, A.R., and Maloney, K.A., Mitochondrial introns and mitochondrial plasmids of Neurospora. In: Achievements and Perspectives in Mitochondrial Research. Volume II: Biogenesis (E. Quagliariello et al., eds.), II: 237-247
1985 Akins, R.A. and Lambowitz, A.M., A general method for cloning Neurospora crassa nuclear genes by complementation of mutants., Mol. Cell. Biol. 5: 2272-2278
1985 Collins, R.A. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Defective splicing of mitochondrial mRNA precursors in nuclear mutant cyt-18-1., J. Mol. Biol. 184: 413-428
1984 Garriga, G. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Self-splicing of a mitochondrial intron in vitro., Cell 39: 631-641
1984 Nargang, F.E., Bell, J.B., Stohl, L.L. and Lambowitz, A.M., The DNA sequence and genetic organization of a Neurospora mitochondrial plasmid suggest a relationship to introns and mobile elements., Cell 38: 441-453
1984 Grant, D.M., Lambowitz, A.M., Rambosek, J.A. and Kinsey, J.A., Transformation of Neurospora with recombinant plasmids containing the cloned glutamate dehydrogenase (am) gene. Evidence for autonomous replication of the transforming plasmid., Mol. Cell. Biol. 4: 2041-2051
1984 Stohl, L.L., Akins, R.A. and Lambowitz, A.M., Characterization of deletion derivative of an autonomously replicating Neurospora plasmid., Nucl. Acids. Res. 12: 6169-6178
1984 Collins, R.A. and Lambowitz, A.M., The physical and genetic map of mitochondrial DNA from Neurospora crassa strain 74-OR23-1A. In: Genetic Maps (S.J. O'Brien, ed.), Vol. III: 274-276
1984 Akins, R.A. and Lambowitz, A.M., The poky mutant of Neurospora contains a four base pair deletion at the 5' end of the mitochondrial small rRNA., Proc. Natl. Acad. Sci. USA 81: 3791-3794
1984 Garriga, G., Bertrand, H., and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Nuclear mutants defective in both splicing and 3' end synthesis of the large rRNA., Cell 36: 623-634
1983 Garriga, G. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria., J. Biol. Chem. 258: 14745-14748
1983 Stohl, L.L. and Lambowitz, A.M., A colony filter hybridization procedure for the filamentous fungus, Neurospora crassa., Anal. Biochem. 134: 82-85
1983 Lambowitz, A.M., Kobayashi, G.S., Painter, A. and Medoff, G., Possible relationship of morphogenesis in pathogenic fungus, Histoplasma capsulatum, to heat shock response., Nature 303: 806-808
1983 Sacco, M., Medoff, G., Lambowitz, A.M., Kumar, B.V., Kobayashi, G.S. and Painter, A., Sulfhydryl-induced respiratory "shunt" pathways and their role in morphogenesis in the fungus, Histoplasma capsulatum., J. Biol. Chem. 258: 8223-8230
1983 Nargang, F.E., Bell, J.B., Stohl, L.L. and Lambowitz, A.M., A family of repetitive GC-rich palindromic sequences found in Neurospora mitochondrial DNA is also found in a mitochondrial plasmid DNA., J. Biol. Chem. 258: 4257-4260
1983 Wollenzien, P.L., Cantor, C.R., Grant, D.M. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria. Structure of the unspliced 35S precursor ribosomal RNA detected by psoralen cross-linking., Cell 32: 397-407
1983 Stohl, L.L. and Lambowitz, A.M., Construction of a shuttle vector for the filamentous fungus, Neurospora crassa., Proc. Natl. Acad. Sci. USA 80: 1058-1062
1983 Collins, R.A. and Lambowitz, A.M., Structural variations and optional introns in the mitochondrial DNAs of Neurospora strains isolated from nature., Plasmid 9: 53-70
1982 Collins, R.A., Grant, D.M. and Lambowitz, A.M., The physical and genetic map of mtDNA from Neurospora crassa strain 74-OR23-1A. In: Genetic Maps (S.J. O'Brien, ed.), Vol. II: 206-207
1982 Grant, D.M. and Lambowitz, A.M., Mitochondrial rRNA genes. In: The Cell Nucleus (H. Busch and L. Rothblum, eds.), Vol. X Part A: 387-408
1982 LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially-assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients., J. Cell Biol. 95: 267-277
1982 Bertrand, H., Bridge, P., Collins, R.A., Garriga, G. and Lambowitz, A.M. , RNA splicing in Neurospora mitochondria. Characterization of new nuclear mutants with defects in splicing the mitochondrial large rRNA., Cell 29: 517-526
1982 Garriga, G., Collins, R.A., Grant, D.M., Lambowitz, A.M., and Bertrand, H., Mitochondrial RNA splicing in Neurospora crassa. In: Mitochondrial Genes (P. Slonimski, P. Borst, and G. Attardi, eds.), 381-390
1982 Stohl, L.L., Collins, R.A., Cole, M.D., and Lambowitz, A.M., Characterization of two new plasmid DNAs found in mitochondria of wild-type Neurospora intermedia strains., Nucl. Acids Res. 10: 1439-1458
1981 Collins, R.A. and Lambowitz, A.M., Characterization of a variant Neurospora crassa mitochondrial DNA which contains tandem reiterations of a 1.9 kb sequence., Curr. Genet. 4: 131-133
1981 LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora. Purification of the mitochondrially-synthesized ribosomal protein, S-5., J. Biol. Chem. 256: 7064-7067
1981 Maresca, B., Lambowitz, A.M., Kumar, V.B., Grant, G.A., Kobayashi, G.S. and Medoff, G., Role of cysteine in regulating morphogenesis and mitochondrial activity in the dimorphic fungus Histoplasma capsulatum., Proc. Natl. Acad. Sci. USA 78: 4596-4600
1981 Collins, R.A., Stohl, L.L., Cole, M.D. and Lambowitz, A.M., Characterization of a novel plasmid DNA found in mitochondria of N. crassa., Cell 24: 443-452
1981 Grimm, M.F., Cole, M.D. and Lambowitz, A.M., RNA splicing in Neurospora mitochondria: Secondary structure of the 35S precursor RNA investigated by digestion with ribonuclease III and by electron microscopy., Biochemistry 20: 2836-2842
1981 Green, M.R., Grimm, M.F., Goewert, R.R., Collins, R.A., Cole, M.D., Lambowitz, A.M., Heckman, J.E., Yin, S. and, RajBhandary, U.L., Transcripts and processing patterns for the ribosomal RNA and transfer RNA region of Neurospora crassa mitochondrial DNA., J. Biol. Chem. 256: 2027-2034
1981 Collins, R.A., Bertrand, H., LaPolla, R.J. and Lambowitz, A.M., A novel extra nuclear mutant of Neurospora with a temperature-sensitive defect in mitochondrial protein synthesis and mitochondrial ATPase., Mol. Gen. Genet. 181: 13-19
1980 Bertrand, H., Collins, R.A., Stohl, L.L., Goewert, R.R. and, Lambowitz, A.M., Deletion mutants of Neurospora crassa mitochondrial DNA and their relationship to the "stop-start" growth phenotype., Proc. Natl. Acad. Sci. USA 77: 6032-6036
1980 Lambowitz, A.M., Mitochondrial ribosome assembly and RNA splicing in Neurospora crassa., The Organization and Expression of the Mitochondrial Genome (A.M. Kroon and C. Saccone, eds.) 291-300
1980 Boynton, J.E., Gillham, N.W., and Lambowitz, A.M., Biogenesis of chloroplast and mitochondrial ribosomes. In: Ribosomes: Structure, Function and Genetics (G. Chamblis, G.R. Craven, J. Davies, K. Davis, L. Kahan and M. Nomura, ed.) , 903-950
1979 Mannella, C.A., Goewert, R.R. and Lambowitz, A.M., Characterization of variant Neurospora crassa mitochondrial DNAs which contain tandem reiterations., Cell 18: 1197-1207
1979 Mannella, C.A. and Lambowitz, A.M., Unidirectional gene conversion associated with two insertions in Neurospora crassa mitochondrial DNA., Genetics 93: 645-654
1979 LaPolla, R.J. and Lambowitz, A.M., The binding of mitochondrial ribosomal proteins to a mitochondrial ribosomal precursor RNA containing a 2.3 kb intron., J. Biol. Chem. 254: 11746-11750
1979 Grimm, M.F. and Lambowitz, A.M., The 32 S RNA of Neurospora crassa mitochondria is not a precursor of the mitochondrial rRNAs., J. Mol. Biol. 134: 667-672
1979 Collins, R.A., Bertrand, H., LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora crassa: Mutants with defects in mitochondrial ribosome assembly., Mol. Gen. Genet. 177: 73-84
1979 Maresca, B., Lambowitz, A.M., Kobayashi, G.S. and Medoff, G., Respiration in the yeast and mycelial phases of Histoplasma capsulatum., J. Bacteriol. 138: 647-649
1979 Mannella, C.A., Collins, R.A., Green, M.R. and Lambowitz, A.M., Defective splicing of mitochondrial rRNA in cytochrome-deficient nuclear mutants of Neurospora crassa., Proc. Natl. Acad. Sci. USA 76: 2635-2639
1979 Lambowitz, A.M., LaPolla, R.J. and Collins, R.A., Mitochondrial ribosome assembly in Neurospora. Two-dimensional gel analysis of mitochondrial ribosomal proteins., J. Cell Biol. 82: 17-31
1979 Mannella, C.A., Pittenger, T.H. and Lambowitz, A.M., Transmission of mitochondrial DNA in Neurospora crassa sexual crosses., J. Bacteriol. 137: 1449-1451
1979 Lambowitz, A.M., Preparation and analysis of mitochondrial ribosomes., Meth. Enzymol. 59: 421-433
1978 Lambowitz, A.M. and Zannoni, D., Cyanide-insensitive respiration in Neurospora; genetic and biophysical approaches. In: Genetics and Biogenesis of Chloroplasts and Mitochondria (T. Bucher et al., ed.), 283-291
1978 Mannella, C.A. and Lambowitz, A.M., Interaction of wild-type and poky mitochondrial DNA in heterokaryons of Neurospora., Biochem. Biophys. Res. Commun. 80: 673-679
1977 LaPolla, R.J. and Lambowitz, A.M., Mitochondrial ribosome assembly in Neurospora crassa; chloramphenicol inhibits the maturation of small ribosomal subunits., J. Mol. Biol. 116: 189-205
1976 Lambowitz, A.M., Chua, N.H. and Luck, D.J.L., Mitochondrial ribosome assembly in Neurospora crassa; preparation of ribosomal precursor particles, site of synthesis of mitochondrial ribosomal proteins, and studies on the poky mutant., J. Biol. Chem. 107: 223-253
1976 Lambowitz, A.M. and Luck, D.J.L., Studies on the poky mutant of Neurospora crassa; fingerprint analysis of mitochondrial ribosomal RNA., J. Biol. Chem. 251: 3081-3095
1975 Lambowitz, A.M. and Luck, D.J.L., Methylation of mitochondrial RNA species in wild-type and poky strains of Neurospora crassa., J. Mol. Biol. 96: 207-214
1974 Bonner, W.D., Jr., Lambowitz, A.M. and Wikstrom, M.K.F. , On the lack of ATP induced midpoint potential shift in cytochrome b-566 in plant mitochondria. In: Dynamics of Energy-Transducing Membranes (L. Ernster, R.W. Estabrook, and E.C. Slater, eds.), 195-200
1974 Lambowitz, A.M. and Bonner, W.D., Jr., The b cytochromes of plant mitochondria. In: Dynamics of Energy-Transducing Membranes (L. Ernster, R.W. Estabrook, and E.C. Slater, eds.), 77-92
1974 Wikstrom, M.K.F. and Lambowitz, A.M., The interaction of redox mediators with the second phosphorylation site; significance for the cytochrome bT hypothesis., FEBS Lett. 40: 149-153
1974 Lambowitz, A.M. and Bonner, W.D., Jr., The mitochondrial b-cytochromes of the wild-type and poky strains of Neurospora crassa; evidence for a component reduced only by dithionite., J. Biol. Chem. 249: 2886-2890
1974 Lambowitz, A.M., Bonner, W.D., Jr. and Wikstrom, M.K.F., On the lack of ATP induced midpoint potential shift for cytochrome b-566 in plant mitochondria., Proc. Natl. Acad. Sci. USA 71: 1183-1187
1974 Lambowitz, A.M. and Bonner, W.D., Jr., The b-cytochromes of plant mitochondria; a spectrophotometric and potentiometric study., J. Biol. Chem. 249: 2428-2440
1973 Lambowitz, A.M. and Bonner, W.D., Jr., The effect of antimycin on the b-cytochromes of plant mitochondria; oxidation-reduction behavior of cytochrome b-566., Biochem. Biophys. Res. Commun. 52: 703-711
1972 Lambowitz, A.M. and Slayman, C.W., Effect of pyrrolnitrin on electron transport and oxidative phosphorylation in mitochondria isolated from Neurospora crassa., J. Bacteriol. 112: 1020-1022
1972 Lambowitz, A.M., Smith, E.W. and Slayman, C.W., Oxidative phosphorylation in Neurospora mitochondria; studies on wild type, poky, and chloramphenicol-induced wild type., J. Biol. Chem. 247: 4859-4865
1972 Lambowitz, A.M., Smith, E.W. and Slayman, C.W., Electron transport in Neurospora mitochondria; studies on wild type and poky., J. Biol. Chem. 247: 4850-4858
1972 Lambowitz, A.M., Slayman, C.W., Slayman, C.L. and Bonner, W.D., Jr., The electron transport components of the wild-type and poky strains of Neurospora crassa., J. Biol. Chem. 247: 1536-1545
1971 Lambowitz, A.M. and Slayman, C.W., Cyanide-resistant respiration in Neurospora crassa., J. Bacteriol. 108: 1087-1096
Basil O'Connor Starter Research Grant from National Foundation-March of Dimes, 1977-1980; NIH Merit Award, 1993; American Academy of Arts and Sciences, 1995; UT Cooperative Society Award for Best Research Paper, 2000; Fellow, American Association for the Advancement of Science, 2001; American Academy of Microbiology, 2002; National Academy of Sciences, 2004; The Academy of Medicine, Engineering, and Science of Texas, 2004; Wilbur Cross Medal, Yale University, 2013.
Institute for Cellular and Molecular Biology