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Тулуковщина — деревня в Клинцовском районе Брянской области, в составе Первомайского сельского поселения.

География
Находится в западной части Брянской области на расстоянии приблизительно 9 км на запад-северо-запад по прямой от железнодорожного вокзала станции Клинцы.

История
Основана Рагузинскими в первой половине XVIII века как слобода, позднее принадлежала графу Румянцеву и другим владельцам, в XIX веке — деревня. До 1781 входила в Новоместскую сотню Стародубского полка. В 1859 году здесь (деревня Суражского уезда Черниговской губернии) учтено было 39 дворов, в 1892—74. Ныне действует Сергиевская церковь.

Население
Численность населения: 246 человек (1859 год), 464 (1892), 204 человека в 2002 году (русские 99 %), 131 в 2010.

Примечания

Населённые пункты Клинцовского района

redpajama_set_name: RedPajamaWikipedia


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Meet the 2015 SXSW Filmmakers #9: Trey Shults Hopes Audiences Will Embrace a Compelling Female Antihero
Jena Keahon
After years of absence, Krisha reunites with her family for a holiday gathering. She sees it as an opportunity to fix her past mistakes, cook the family turkey, and prove to her loved ones that she has changed for the better. Only, Krisha's delirium takes her family on a dizzying holiday that no one will forget [synopsis courtesy of SXSW]. Trey Shults shares details about the making of his new film "KIRSHA," based off his short film that was awarded Special Jury Recognition at SXSW 2014.
What's your film about in 140 characters or less?
A woman returns to her family after years of absence, and the only things that stand in her way are her family, dogs, and turkey. In actuality, the only thing standing in her way is her self.
Now what's it REALLY about?
Family, love, hate, addiction, motherhood, confronting demons — It's about someone who is at war with their own self and the effect it can have on the ones they love the most.
Tell us briefly about yourself.
I live in good ole Montgomery, Texas, which I just found out is the birthplace of the Texas state flag. I live in my parent's house where we shot the movie. I have three cats: technically one, because the other two are my girlfriend's. My girlfriend is a flight attendant, so I get the huge benefit of flying for free, which is a big plus for a filmmaker. I love cinema, and want nothing more than to be able to make personal films for a living. "KRISHA" is my first feature film, and it was made with friends and family.
Biggest challenge in completing this film?
I think the speed in which we made it. The short film played SXSW just a year ago, and we didn't decide to fully embrace a feature until after the festival and the short's good reception. Overall, making this film was one of the best experiences of my life, and we accomplished a lot in a year, but it came together very fast. Giving all that I had into this movie, while trying to maintain a day job and normalcy in life was tricky. Also, being as ambitious as we were with our budget was tricky.
What do you want the SXSW audience to take away from your film?
I hope they go on this journey with Krisha, and really enjoy the range of emotions the film goes through. So, I really hope audiences embrace the comedy, intensity, and tragedy of the film. Hopefully, they are impacted by the film and feel like it's something they haven't seen before. I'd like them to see Krisha as a really compelling female antihero, which I feel we don't get enough of. A ton of love and care was put into the film, and I just hope that comes across in the final product.
Any films inspire you?
So many. For this particular film, I would say: "A Woman Under the Influence," "Festen," "Raging Bull," "Punch-Drunk Love," "There Will Be Blood," "Pusher 3: I'm the Angel of Death," "The Shining," and many more I can't think of.
I have a script written that I'm dying to make. It's my version of a horror film. It takes the emotional intensity of "KRISHA," and applies it to a horror film. I wrote it after my dad died. So, it applies themes of fear, death, and family.
What cameras did you shoot on?
RED Epic and RED Scarlet.
Did you crowdfund?
If so, via what platform.
Yes — we ran a Kickstarter campaign.
Did you go to film school? If so, which one?
Nope. I've just spent a lot of time watching movies, making my own short films, and learning from my mistakes.
Indiewire invited SXSW Film Festival directors to tell us about their films, including what inspired them, the challenges they faced and what they're doing next. We'll be publishing their responses leading up to the 2015 festival. For profiles go HERE.
This Article is related to: Festivals and tagged SXSW 2015, SXSW Meet the Filmmakers 2015

redpajama_set_name: RedPajamaCommonCrawl


Trying to find someone to train or assess you? This link lists all the RTOs that are currently registered to deliver SIFBGM003B, 'Clean and maintain public conveniences and amenities'.
Select and assemble cleaning agents and equipment.
Liaise with appropriate staff to identify and confirm work requirements.
Prepare and use personal protective equipment according to workplace policies and procedures, and relevant legislation.
Check working order of equipment.
Dilute required chemicals according to manufacturer instructions.
Clean and maintain conveniences and amenities.
Clean lavatories, basins and floors according to workplace policies and procedures, and relevant legislation.
Check and replace consumables according to workplace policies and procedures.
Empty and clean waste bins according to workplace policies and procedures.
Identify hazardous waste and take corrective action according to workplace policies and procedures, and relevant legislation.
Complete cleaning schedule documentation according to workplace policies and procedures.
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Store and monitor cleaning agents and equipment.
Store cleaning agents and cleaning equipment according to workplace policies and procedures.
Check supplies of cleaning agents and cleaning equipment according to workplace policies and procedures.
Order supplies of cleaning agents and cleaning equipment according to workplace policies and procedures.


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Co-founder and Executive Chairman, Alibaba Group
Jack Ma is Alibaba's lead founder and, since May 2013, its executive chairman. From the founding in 1999 until May 2013, Jack served as chairman and chief executive officer. He is also the founder of the Zhejiang-based Jack Ma Foundation. Jack currently serves on the board of SoftBank Corp., a Japanese corporation listed on the Tokyo Stock Exchange. He is also a member of the Foundation Board of the World Economic Forum, chairman of the Zhejiang Chamber of Commerce, as well as chairman of the China Entrepreneur Club. In January 2016, he was named a Sustainable Development Goals (SDGs) advocate by the United Nations. In September 2016, he was named special adviser of the United Nations Conference on Trade and Development (UNCTAD) for Youth Entrepreneurship and Small Business, which holds the rank of assistant secretary-general of the United Nations. Ma graduated from Hangzhou Teacher's Institute with a major in English language education.
@AlibabaGroup
Appearances by Jack Ma
October 12, 2018 2:30
Disrupting Development: Digital Platforms and Innovation
DigitalDev


Dazzling Daisy Earring Studs - PANDORA is rated 4.9 out of 5 by 20.
Rated 5 out of 5 by amandat64 from Beautiful! These earrings are girly and gorgeous! I've bought several pair of stud earrings from Pandora but I think these are my favorites. If you purchase them, I'm pretty sure you won't be disappointed.
Rated 5 out of 5 by Isabelpr7 from Daizzling Daisy Bellas. Me encantaron, llegaron super rápido. las había visto y pensé que eran mas pequeñas, son perfectas.
Rated 5 out of 5 by happy2 from cute Bought these for myself to go with Dazzling Daisy ring, they are very cute in person, well-made closure so they're hard to loose and they came in adorable package! Very happy with my purchase.
Rated 5 out of 5 by Shellbee38 from She's a happy camper. I bought these for my daughter to go with her ring and charm bracelet. She is very happy with them.
Rated 5 out of 5 by Antonia Aponte from Beautiful I love the back lock... I always loose my earings everywhere. With this ones I feel that they are secure and in place.
Rated 5 out of 5 by Carr from They are just perfect! I bought them as a present for my niece's birthday and she just loved them. They are the perfect size, they are simply perfect!
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On August 14th 1967, The Marine Offences Act was passed by Harold Wilson's government, silencing all the pirate ships on the North Sea except one. Radio Caroline from the MV Mi-Amigo continued after Midnight that evening and indeed as far as 1980, when sadly the Mi-Amigo sank. In 1983, Caroline gained a new lease of life, via radio ship the MV 'Ross Revenge', which in turn stayed out on the north sea broadcasting live until 1990.
Also, since the late 60's, land based pirates on Medium Wave and later FM, filled the airwaves with alternatives to the drab (lack of) choice, that UK radio offered.
Proud to be a part of what was... Overflow Radio was landbased between Dec 1986 - Feb 2001, broadcasting on FM in various locations, a strict diet of alternative and underground music, from the sublime to the unreal!
Here in 2008, The Overflow can be heard via two legal stations, Radio Seagull & River Gibbs FM. In the pirate days, it was just weekends. A saturday, a sunday... maybe an odd day in the week when time permitted. Although the pirate days and total freedom of speech are sadly missed, we can now be heard 7 days a week, so all is not lost.
The main dissapointment of the last 20 years or so, is that radio seems to have learned no lessons in this country. The government bodies that were and now are, seem to have glossed over everything that was being fought for and we are now more or less back where we began. In the eighties, Laser 558 gave the system a massive kick up the a**e, the biggest it had experienced since 1967, yet here we are again in 2008, in a completely stale world of un-interesting babbling radio, with no choice that we want and little if anything to listen to at all!
Although there is nothing financial to be gained, if any member of The Overflow won the lottery tomorrow, we would guarantee a ship on the North Sea within hours, broadcasting a strict diet of intelligent, non industry-produced proper music, dispersed with ego-less, amusing and vaguely-interesting banter! None of which you will find on ILR or BBC Radio.
Oh well.... same time next year???


It's understandable why some Celtics fans want to spew venom towards Kyrie Irving. The dazzling point guard appeared to publicly retract his preseason promise of re-signing here, telling reporters earlier this month he doesn't owe "anybody bleep" months before his pending free agency.
On top of that, the Irving-to-New York rumors may have been part of some larger Rich Paul-orchestrated conspiracy to force Anthony Davis to the Lakers, the Sporting News reports.
It's frustrating to watch unfold, and makes it seem as if Irving is toying with the Celtics for personal amusement. The C's playing well in Irving's absence gives us plenty of ammunition to use against him.
But it's an illogical argument based on nothing more than anecdotal evidence. Yes, the Celtics are 10-2 without Irving this season, after heading to Game 7 of the Eastern Conference Finals without him last year. They beat the Sixers and Pistons with Irving sidelined this week with a right knee issue.
On the contrary, they blew a 28-point lead to the Clippers while Irving was out. It was one of the more humiliating losses in recent franchise memory.
All feeble arguments about how the Celtics are better without Irving –– which callers have repeated on sports talk airwaves across the city all week long –– ignore how well they played with him last season. They went 41-19 with Irving leading them during the 2017-18 campaign, before a season-ending left knee injury forced him to the bench.
Irving's balky knees are a far more convincing strike against him than any nonsensical proclamation the Celtics would be better off without one of the league's top-10 players. You need premier talent to win in the NBA. Irving is the only player on the roster who qualifies as such.
Also, anybody who claims to want to jettison Irving needs to explain the end game. Getting rid of Irving would seemingly make it impossible to convince Davis of signing an extension to stay in Boston. Last year's playoff run was fun, but you're probably not getting back to the Eastern Conference Finals with Terry Rozier starting at point guard –– even with Gordon Hayward in the lineup.
This is about championships, and an Irving-Davis collaboration instantly boosts the Celtics to the top of the East, even if Jayson Tatum heads to New Orleans. The C's can offer Irving $80 more million than anybody else in free agency and own the best chips to land Davis. That is still their most desirable path forward.
For the Celtics' sake, hopefully their partnership with Irving is just beginning, despite what some fluke wins have to say.


text:
Cincinnati-made movie gets local debut
Updated: 6:25 PM EST Dec 8, 2016
WLWT Digital Staff
A Cincinnati-made movie will get a Queen City debut. "A Kind of Murder" filmed across the city in 2015. And filmmakers are returning to Cincinnati for its premier. Before the film's national release, it will premiere Dec. 15 at Esquire Theater. Before the showing, Film Cincinnati will update audience members on films coming to the Queen City in 2017. "A Kind of Murder" is a thriller based on the Patricia Highsmith novel "The Blunderer." It stars Patrick Wilson, Jessica Biel, Vincent Kartheiser and Haley Benn. The screening is set for 4:30 p.m.
A Cincinnati-made movie will get a Queen City debut.
"A Kind of Murder" filmed across the city in 2015. And filmmakers are returning to Cincinnati for its premier.
Before the film's national release, it will premiere Dec. 15 at Esquire Theater. Before the showing, Film Cincinnati will update audience members on films coming to the Queen City in 2017.
"A Kind of Murder" is a thriller based on the Patricia Highsmith novel "The Blunderer." It stars Patrick Wilson, Jessica Biel, Vincent Kartheiser and Haley Benn.
The screening is set for 4:30 p.m.


At Altacode we are primarily focused on providing hi-end and effective custom software design, development and implementation services with utilization of leverage manpower and advanced technologies. We carry out the full cycle of custom application development, from concept design through technical support.
This includes a comprehensive process of requirements gathering, system design and architecture, iterative development, quality assurance, documentation preparation, technical support and maintenance.


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General Microbial Ecology
Detritus-Dependent Development of the Microbial Community in an Experimental System: Qualitative Analysis by Denaturing Gradient Gel Electrophoresis
Erik J. van Hannen, Wolf Mooij, Miranda P. van Agterveld, Herman J. Gons, Hendrikus J. Laanbroek
Erik J. van Hannen
Department of Microbial Ecology, 1 and
Wolf Mooij
Department of Food Web Studies, 2 Centre for Limnology, Netherlands Institute of Ecology, 3600 BG Maarssen, The Netherlands
Miranda P. van Agterveld
Herman J. Gons
Hendrikus J. Laanbroek
Correlations between the biomass of phytoplankton and the biomass of bacteria and between the biomass of bacteria and the biomass of protozoans suggest that there is coupling between these compartments of the "microbial loop." To investigate this coupling on the species level, bacteria and protozoans from untreated lake water inocula were allowed to grow on detritus of the green alga Ankistrodesmus falcatus or the cyanobacterium Oscillatoria limneticain continuous-flow systems for 1 month. Denaturing gradient gel electrophoresis (DGGE) of the 16S and 18S rRNA genes was used to monitor the development of the bacterial community structure and the eukaryotic community structure, respectively. Nonmetric multidimensional scaling of the DGGE profiles revealed the changes in the microbial community structure. This analysis showed that significantly different bacterial communities developed on the green algal detritus and on the cyanobacterial detritus. Although similar results were obtained for the eukaryotic communities, the differences were not significant. Hence, our findings indicate that the origin of detritus can affect the structure of at least the bacterial community. A phylogenetic analysis of 20 18S ribosomal DNA clones that were isolated from the continuous cultures revealed that many sequences were related to the sequences of bacterivorous protozoans (members of the Ciliophora, Rhizopoda, Amoeba, and Kinetoplastida). One clone grouped in a recently established clade whose previously described members are all parasites. The affiliations of about 20% of the clones could not be determined.
In aquatic environments, photosynthetically produced organic carbon is decomposed by heterotrophic bacteria, which in turn are consumed by heterotrophic protozoans. Correlations between phytoplankton biomass and heterotrophic bacterial biomass (10, 12, 41) and between heterotrophic bacterial biomass and protozoan biomass (8, 9, 11,28) suggest that there is coupling between the compartments of the so-called microbial loop (4). However, most descriptions of such relationships have been based on bulk measurements of the compartments, and whether there are correlations at the species level is an intriguing question. For example, is organic carbon derived from different phytoplankton species decomposed by different species of specialized bacteria? Or is protozoan species composition governed by bacterial species composition or just by the concentration of edible food particles? Although numerous studies have shown that bacteria have different rates of growth on natural carbon sources (6, 7, 16, 21,32, 33) and that heterotrophic protozoans selectively graze on larger bacteria (15, 29, 31), little is known about whether the development of aquatic microbial communities depends on various organic carbon sources. This lack of information is attributable to the difficulty of determining the presence of microbial species due to the fact that the majority of these microorganisms cannot be grown with the current cultivation methods. Molecular techniques can be used to assess genetic composition without culturing all members of a community. To investigate the potential dependence of microbial community structure on the source of detritus in lakes, we used denaturing gradient gel electrophoresis (DGGE). We determined the bacterial community structure and the eukaryotic community structure (22, 35) for communities that developed on detritus derived from the green algaAnkistrodesmus falcatus or on detritus derived from the cyanobacterium Oscillatoria limnetica in specially designed two-stage continuous-flow systems (34). Since DGGE does not provide quantitative results (38), we converted the band patterns to reflect whether particular sequence types were present or absent. Our qualitative measures of microbial community structure were analyzed by nonmetric multidimensional scaling (NMDS) and a statistical method used for comparisons of the DGGE patterns. Special attention was paid to the eukaryotic microbial community. Since most heterotrophic protozoans are bacterivorous, we wanted to determine whether the carbon source used by the bacteria also influenced the community structure of the protozoans.
Experimental setup.Nonaxenic A. falcatus (Centre for Limnology strain E01) and Oscillatoria cf.limnetica (Centre for Limnology strain MR1) were grown under light-limiting conditions in modified Guillard medium (34) in the first stages (volume, 2 liters) of two continuous-flow systems (designated the Alga system and the Cyano system) until the steady state was reached (dilution rate, 0.36 day−1). The two-stage continuous-flow system has been described previously (34). The cultures were grown at a constant temperature (20°C) at pH 8.0 ± 0.2 with illumination by type TLE 32W/33 circular fluorescent lamps (Philips). The inner sides of the lamps facing the culture vessels were shielded with aluminum foil to decrease the light intensity. The light intensity for theAnkistrodesmus cultures (Alga system) was manipulated so that the chlorophyll a (Chla) concentrations in the two systems were approximately the same. Samples (10 ml per assay) were removed every 24 h and used to determine the concentrations of Chla and total suspended solids (TSS).
After 30 days the cultures were considered to be steady-state cultures based on the TSS and Chla concentrations. The two second stages (volume, 1 liter) (the replicates were designated Alga 1, Alga 2, Cyano 1, and Cyano 2) of each flow system were then filled with water from Lake Ketelmeer (The Netherlands) and connected to the first stages. Lake Ketelmeer is a small turbid lake (surface area, 35 km−2; mean depth, 3 m) with a short water retention time (∼1 week). This lake water was used as an inoculum for bacteria and heterotrophic eukaryotes and was chosen because both cyanobacteria and green algae are present in Lake Ketelmeer (36). If the origin of detritus governs microbial community structure, the lake water inoculum should have contained microorganisms that were specialized for decomposing these carbon sources. The second stages received UV-C-killed phytoplankton from the first stages at a dilution rate of 0.30 day−1. The UV-C intensities needed to killO. limnetica and A. falcatus were 5 and 18 W · m−2, respectively. The procedures used to assess lethal UV-C intensities have been described previously (34). For 1 month, samples were removed and used for Chla, TSS, and community structure analyses (DGGE and 18S rRNA sequence determination).
Chla and TSS.The Chla and TSS assays used have been described previously (34). Duplicate measurements were obtained with each assay.
DNA extraction, PCR, and DGGE.DNA was released from cells by mechanical force (bead beating), phenol extraction, and ethanol precipitation (42). PCR primers for the V2 region were used for amplification of the 16S rRNA gene. The PCR primers used were F357GC (5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCCCTACGGGAGGCAGCAG-3′), which contains a GC-rich clamp and is specific for most members of the Bacteria, and R518 (5′-ATTACCGCGGCTGCTGG-3′), which is specific for most members of the Bacteria,Archaea, and Eucarya (22). Each PCR amplification was performed in a 50-μl reaction mixture containing approximately 100 ng of template DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 0.01% (wt/vol) gelatin, 1.5 mM MgCl2, each primer at a concentration of 0.5 μM, each deoxynucleotide at a concentration of 200 μM, 400 ng of bovine serum albumin, and 2.5 U of TaqDNA polymerase (Boehringer, Mannheim, Germany). PCR cycling was performed with a Perkin-Elmer model 480 thermocycler. The temperature cycling conditions were as follows: preincubation at 94°C for 5 min, followed by 25 cycles consisting of 94°C for 1 min, the annealing temperature (TA) for 1 min, and 72°C for 1 min. In the first 20 cycles the TA was decreased by 1°C stepwise each two cycles, from 65°C in the first cycle to 56°C in the 20th cycle. In the last five cycles the TA was 55°C. The 25 cycles were followed by 5 min of incubation at 72°C. The primers used for amplification of the 18S rRNA gene were F1427GC (5′-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCTCTGTGATGCCCTTAGATGTTCTGGG-3′) and R1616 (5′-GCGGTGTGTACAAAGGGCAGGG-3′). Both of these primers are specific for eukaryotic aquatic microorganisms (35). The temperature cycling conditions were as follows: preincubation at 94°C for 5 min, followed by 25 cycles consisting of 94°C for 1 min, 52°C for 1 min, and 72°C for 1 min and then a final extension step consisting of 72°C for 5 min. The reaction conditions were the same as described above. DGGE was performed as described previously (35, 42). Briefly, PCR products of similar sizes were separated on a 1.5-mm-thick vertical gel containing 8% (wt/vol) polyacrylamide (acrylamide/bisacrylamide ratio, 37.5:1) and a linear gradient consisting of the denaturants urea and formamide; the concentration of the denaturants increased from 35% at the top of the gel to 55% at the bottom for separation of the 16S ribosomal DNA (rDNA) fragment and from 30 to 55% for separation of the 18S rDNA fragment (100% denaturant was defined as 7 M urea and 40% [vol/vol] formamide). Equal amounts of PCR products were applied to the DGGE gel. The concentrations of PCR products were estimated by separating the products on 2.0% agarose gels, staining them with ethidium bromide (see below), and analyzing digitized images with ImageQuant software (Molecular Dynamics Ltd., Kemsing, England). A 50-μl portion of the sample containing the smallest amount of PCR product was loaded onto the DGGE gel; all other samples were loaded in amounts relative to this sample. Electrophoresis was performed at 60°C with a buffer containing 40 mM Tris, 40 mM acetic acid, and 1 mM EDTA (pH 7.6) (0.5× TAE buffer), and 75 V was applied to the submerged gel for 16 h. Nucleic acids were visualized by staining the gel for 1 h in 0.5× TAE buffer containing 0.5 mg of ethidium bromide per liter and then destaining it for 5 min in demineralized water and photographing it with a charge-coupled device camera (The Imager; Appligene, Illkirch, France). Digitized images were inverted by using the Photostyler software (Aldus Corporation, Seattle, Wash.). The contrast and gray balance of the entire image were adjusted to reduce the background.
Clone library construction.To estimate the eukaryotic species present in the continuous-flow systems, the DNA extracted from three samples (Alga 1 stage on day 23, Alga 2 stage on day 23, and Cyano 1 stage on day 29) were mixed and used for clone library construction. 18S rDNA clones that were nearly full length were generated by using the Eucarya-specific primers E4 (5′-CTGGTTGATTCTGCCAGT-3′) and E1628 (5′-CGACGGGCGGTGTGTA-3′) (the numbers in the designations indicate Saccharomyces cerevisiae sequence positions). Each PCR amplification was performed in a 50-μl reaction mixture containing approximately 100 ng of template DNA, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 0.01% (wt/vol) gelatin, 1.5 mM MgCl2, each primer at a concentration of 0.5 μM, each deoxynucleotide at a concentration of 200 μM, 400 ng of bovine serum albumin, and 2.5 U ofTaq DNA polymerase (Boehringer). PCR cycling was performed with the Perkin-Elmer model 480 thermocycler programmed as follows: denaturation at 94°C for 5 min, followed by 25 cycles consisting of 94°C for 1 min, 54°C for 1 min, and 72°C for 1 min and a final extension step consisting of 68°C for 10 min. The cloning and sequencing procedures used have been described previously (42). A total of 120 clones were examined by DGGE. Only clones whose DGGE band positions were different were sequenced. The sequences were determined in two directions by using the following Texas red-labeled primers: M13 forward (5′-TGTAAAACGACGGCCAGT-3′), M13 reverse (5′-GAAACAGCTATGACCATG-3′), E382 forward (5′-CGGAGAGGGAGCCTGAG-3′), E565 reverse (5′-ATTACCGCGGCTGCTGG-3′), E1128 forward (5′-AAACTTAAAGGAATTGACG-3′), and E1179 reverse (5′-CCCGTGTTGAGTCAAATT-3′), (the numbers in the designations indicate S. cerevisiae sequence positions).
Phylogenetic analysis.The 18S rDNA sequences obtained were compared with sequences obtained from GenBank/EMBL by using BLAST (1). The sequences with the highest levels of similarity were used as reference sequences for alignment. The alignment was based on secondary structure and was constructed by using the Dedicated Comparative Sequence Editor (13). Phylogenetic trees were constructed by using only unambiguously aligned sequence positions and maximum-likelihood analysis (PAUP*, test version 4.0d59; David L. Swofford, Laboratory of Molecular Systematics, Smithsonian Institution, Washington, D.C.). Each analysis was performed five times. Nucleotide frequencies and transition-to-transversion ratios were estimated from the data. Nucleotide substitution rates were assumed to follow a gamma distribution with a shape parameter of 0.5 and setting according to the HKY model (18). "Tree-bisection-reconnection" was used as a swapping algorithm. Maximum-parsimony bootstrap analysis (1,000 replicates) was used to assess the robustness of the trees.
Data analysis.The DGGE patterns were converted to a binary (01) matrix (35). A Nei-Li distance matrix was calculated from the binary data (23). This distance matrix was analyzed by using the NMDS package from the Statistica software package (StatSoft, Inc., Tulsa, Okla.). This procedure presented the data in a Euclidean plane such that very similar values were plotted close together. The resulting graphical representation (NMDS map) was much easier to interpret than the original table of distances was. When it was applied to DGGE data, the NMDS map showed every band pattern (a reflection of the community structure at a particular point in time) as one point, and relative changes in community structure could be visualized and interpreted by connecting consecutive points (37,38). The statistical significance of the differences in community structure due to the source of detritus was determined by using the Nei-Li distances and comparing the communities only at the same moment in time. Two communities fed with different types of detritus were considered to be different at a given moment in time if their Nei-Li distance was significantly greater than the Nei-Li distances between replicate communities. For each of the two sources of detritus we used two replicates and made six observations at different times. On the first sampling day (day 3) the distances between replicates were exceptionally small compared to the distances on later days, indicating that there was a lack of independence from the shared inoculum. Therefore, these distances were not included in the calculations. Thus, a total of 10 distances between replicates were used for the bacterial and eukaryotic community to calculate an average distance between replicates ( ̅D̅i̅s̅ ̅ ̅r̅e̅p̅l̅.̅ ̅ ̅). Each distance between communities growing on different sources of detritus was tested against this distribution and was considered significant ifDisalga-cyano,t > ̅D̅i̅s̅r̅e̅p̅l̅.̅ ̅ ̅ + (T · Stddis-repl. ), whereDisalga-cyano,t is the distance between two communities fed with algal or cyanobacterial detritus at timet, T is the Student t value (n = 9; P = 0.05), andStddis-repl. is the standard deviation of ̅D̅i̅s̅ ̅ ̅r̅e̅p̅l̅.̅ ̅ ̅.
Nucleotide sequence accession numbers.The eukaryotic clone sequences have been deposited in the EMBL database under accession no.AJ130849 to AJ130869.
Decomposition of detritus.The Chla and TSS concentrations in both first stages (Alga system and Cyano system) are shown in Fig. 1. Although the first stages of both flow systems were at a steady state at the beginning of the experiment, in the Alga system the Chla concentration increased after 3 days, while the TSS concentration was constant for the first 15 days. In the Cyano system the Chla level was constant throughout the experiment, while the TSS concentration decreased during the first 15 days. As a result, the two systems had different Chla concentrations after day 15, but their TSS concentrations were about the same. On a dry weight basis, the second stages of the two flow systems received approximately the same amounts of UV-killed biomass after 2 weeks.
Concentrations of Chla and TSS in the two continuous-flow systems (Alga and Cyano systems). The asterisks indicate increases in the Chla and TSS concentrations that were caused by a pump defect. l, liter; d, day.
The patterns of decomposition in all of the second stages were similar. Theoretically, if the decomposition rate is zero, the concentrations of Chla and TSS in the second stages should increase until the levels in the first stage are reached. As soon as the Chlaconcentration started to decrease, the second stages became yellowish instead of green. In the Alga system, the decomposition rate exceeded the inflow of UV-killed cells after approximately 10 days, while in the Cyano system this situation occurred after 5 days. The sharp increases in the Chla and TSS concentrations in the Alga 2 stage were caused by a defective pump. The increases in the Chla and TSS concentrations in the Cyano 2 stage were apparently due to changes in the decomposition rate, and the green color reappeared in this stage.
Microbial community structure.In the 16S rDNA DGGE analysis (Fig. 2A) 54 different bands were detected, while 58 different bands were detected in the 18S rDNA DGGE analysis (Fig. 2B). The relative changes in the structures of the bacterial and eukaryotic communities, as visualized by NMDS, are shown in Fig. 2C and D. Three days after the experiment started, the bacterial communities (Fig. 2C) growing on different types of detritus were still quite similar. A similar observation was made for the eukaryotic communities (Fig. 2D). A clear divergence between the bacterial communities grown on different types of detritus occurred on day 7. This trend persisted, and on day 17 the differences became significantly greater than the differences between the replicates (P < 0.05). Although the structures of the eukaryotic communities also changed, the trend was not as clear as the trend in the bacterial communities, and the differences between the replicates were as great as the differences between the treatments (green algal detritus versus cyanobacterial detritus).
Structures of the microbial communities depending on the source of detritus (green algae or cyanobacteria). (A) 16S rDNA-defined (bacterial) community, as revealed by DGGE. (B) 18S rDNA-defined (eukaryotic) community, as revealed by DGGE. (C) NMDS map of the bacterial communities grown on different types of detritus. (D) NMDS map of the eukaryotic communities grown on different types of detritus. Symbols: ○, Alga 1 stage; □, Alga 2 stage; ●, Cyano 1 stage; ■, Cyano 2 stage. The lines connect the community structures of replicate stages. The numbers indicate the numbers of days from the beginning of the experiment. Boldface numbers indicate significant differences (P < 0.05) in community structures between different sources of detritus. The white bands in panel B, lane c, indicate the band positions for the 18S rDNA clones (Table 1). For each of block of lanes, data for the six times when samples were obtained are shown in consecutive lanes. The arrow in panel A indicates the band belonging toO. limnetica; this band was not included in the Nei-Li distance calculation.
Eukaryotic phylogeny.Of the 120 clones analyzed, 20 were found to have different DGGE bands. The sequences of these 20 clones were determined and phylogenetically analyzed by maximum-likelihood and parsimony methods (Fig. 3), and the frequency with which a band was found at the same position as the clones in the different stages of the two systems is shown in Table1. Since 58 different band positions were identified on the 18S rDNA DGGE gels, our clone library accounted for at most one-third of the total eukaryotic diversity in the two systems. Of the 20 clones, 5 belonged to the Ciliophora (Fig. 3A), 3 belonged to the Rhizopoda (Fig. 3C), 3 belonged to the Metazoa (Fig. 3D), 1 belonged to the Drips (Fig. 3D), 1 belonged to the Amoeba (Fig. 3B), 1 belonged to the fungi (Fig. 3B), and 1 belonged to the Kinetoplastida (Fig. 3E), and the affiliations of 4 clones were not determined. Three of the sequences formed a unique terminal cluster with the fungi as the closest relatives (Fig. 3B).
Maximum-likelihood trees showing the phylogenetic positions of the 18S rDNA clones. The trees are rooted. The numbers indicate the levels of bootstrap support (percentages of 1,000 replicates) obtained in a parsimony analysis. (A) Tree constructed by using S. cerevisiae sequence positions 22 to 641 and 744 to 1643. (B) Tree constructed by using S. cerevisiae sequence positions 50 to 641 and 744 to 1643. (C) Tree constructed by usingS. cerevisiae sequence positions 25 to 641 and 744 to 1643. (D) Tree constructed by using S. cerevisiae sequence positions 50 to 641 and 744 to 1643. (E) Tree constructed by usingLeishmania donovani sequence positions 25 to 993, 1102 to 1300, and 1399 to 2067. Within the order Kinetoplastida, Bodo caudatus can be used as an outgroup (14). The EMBL accession numbers for the sequences are as follows: Gymnodinium beii, U37365; Alexandrium tamarense, X54946;Sarcocystis muris, M64244; Theileria parva,L02366; Glaucoma chattoni, X56533; Tetrahymena nanneyi, X56169; Opisthonecta henneguyi, X56531;Colpoda inflata, M97908; Blepharisma americanum,M97909; Hartmanella vermiformis, M95168;Acanthamoeba castellanii, M13435; Balamuthia mandrillaris, AF019071; Chytridium confervae, M59758;Saccharomyces cerevisiae, Z75578; Tilletia caries, U00972; Chlorarachnion reptans, X70809;Chlorarachnion sp. 1, U02075; Thaumatomonas sp.,U42446; Paulinella chromatophora, X81811; Euglypha rotunda, X77692; Cercomonas 3, U42449;Cercomonas, U42450; Cercomonas 2, U42451;Heteromita globosa, U42447; Ichthyophonus hoferi,U25637; Psorospermium haeckelii, U33180; "Prototheca richardsi," AF07445; Unknown L29455, L29455;Dermocystidium salmonis, U21337; Dermocystidiumsp., U21336; Chaetonotus sp., AJ001735; Artemia salina, X01723; Brachionus plicatilis, U49911;Bodo caudatus, X53910; Trypanoplasma borreli,L14840; Blastocrithidia culicis, L29265;Phytomonas sp., L35076; Leptomonas sp., X53914;Crithidia fasciculata, X03450; Endotrypanum monterogeii, X53911; Leishmania donovani, X07773;Trypanosoma brucei, M12676; and Trypanosoma cruzi, X53917.
Identities and frequencies of occurrence (on six sampling dates) of the 18S rDNA clones in the second stages of our systems
Most bands that were produced by the clones sequenced were not restricted to one of the two systems. Clone LKM36, a clone whose sequence was related to Ciliophora sequences, was the only clone that was frequently found only in the Alga system. Clones LKM48 and LKM118 were also found only in the Alga system. However, these clones were detected only once (Table 1).
We performed an experiment in which we investigated whether the origin of detritus can be a factor that governs microbial community structure. Although we found differences between replicates of the bacterial community growing on the same type of detritus, the effect of the carbon source (green algal detritus versus cyanobacterial detritus) was significantly greater (Fig. 2). In contrast to the bacterial community, the eukaryotic community developed rather chaotically; i.e., the differences between detritus sources were obscured by differences between replicates. This might be interpreted as due to poor reproducibility of the microcosm experiments and to the fact that development of microbial communities at the physical scale which we used is not determined solely by culture conditions, such as light, temperature, and nutrients. However, it is too soon for general conclusions to be made.
The abundance of a sequence was not included in our measurements of community structure since we used only the presence or absence of a DGGE band. Therefore, the changes in the community structure which we measured reflected only changes in species composition, not changes in species abundance. In addition, changes in the community structure, as detected by NMDS analysis, were influenced by the total number of bands in a DGGE pattern and by the number of bands shared by DGGE patterns. Differences in community structure could, therefore, have originated from differences in species diversity (the number of different DGGE bands obtained for two communities) or from differences in species richness (the total number of DGGE bands per community). In general, the bacterial richness of the Alga systems was somewhat greater than the bacterial richness of the Cyano systems. However, the differences in community structure that depended on the source of detritus were largely due to differences in bacterial diversity. The eukaryotic richness fluctuated greatly between sampling days and in all second stages. Thus, differences in the eukaryotic community structure between duplicates and between sources of detritus originated from variations in both eukaryotic richness and diversity.
Our results show that the origin of detritus can affect the structure of the microbial community, at least the bacterial community in our systems. Variations in the chemical composition of phytoplankton (25, 27, 30) and in bacterial substrate utilization may explain the effects on the bacterial community. Although some authors have described chemosensory feeding of bacterivorous protozoans (40), there is very strong evidence that size-selective feeding by protozoans occurs (15, 29, 31). If protozoan prey selection is based solely on size, one might not expect that detritus would have a strong effect on eukaryotic community structure unless the size spectrum of the bacterial community also changes due to the origin of the detritus.
Although we observed no significant differences between the eukaryotic communities growing on the different types of detritus, it was interesting to gain insight into the phylogenetic positions of the protozoan species developing in the flow systems, especially since eukaryotic microorganisms are largely neglected in molecular ecology. Since we constructed our clone library from three samples obtained on two sampling dates, this library does not represent the complete eukaryotic community. We obtained 20 different clones, while eukaryotic DGGE produced 58 different bands. Thus, our library accounts for one-third of the DGGE-detected eukaryotic diversity. Surprisingly, the clone library did not include A. falcatus. Apparently, the high UV-C intensity (18 W · m−2) needed to kill this alga destroyed its DNA. The phylogenetic position of the 18S rDNA clones was inferred by maximum-likelihood analysis (Fig. 3). Replicate analyses (n = 5) always resulted in the same phylogenetic tree. Since the second stages were kept in the dark, there was no algal and or cyanobacterial growth, and bacterivory or uptake of detritus or dissolved organic matter was the only possible mode of feeding. Not surprisingly, most of the sequences found were related to sequences of bacterivorous eukaryotes (members of the Ciliophora, Amoeba Rhizopoda, and Kinetoplastida). Microscopic observations confirmed that these bacterivorous eukaryotes were present. However, we did not identify species. Two sequences (LKM80 and LKM85) were closely related to the sequence of Brachionus plicatilis, a rotifer species capable of feeding on all members of the microbial loop (3). One sequence (LKM88) was closely related to the sequence of a Chaetonotus sp. (Gastrotricha). Since we did not prefilter the lake water inoculum, the metazoan grazers were not eliminated and could develop in the second stages of the flow systems. Three sequences (LKM11, LKM15, and LKM46) were not related to any of the eukaryotic sequences in the EMBL database and formed a unique terminal clade supported by a moderately strong parsimony bootstrap value. The closest relatives of these sequences were the fungi (Fig. 3B). We provisionally designated this cluster LKM11 after the first clone. Another sequence, LKM118, was not related to any previously described eukaryotic sequence. Phylogenetic analysis placed the LKM118 sequence just below the "crown" of the eukaryotic tree, although this position was very unstable (data not shown).
One sequence (LKM51) is particularly interesting. This sequence grouped in a recently established phylogenetic clade, the DRIPs clade, which was provisionally named after its first members (26). All of the sequences currently in this cluster are from protistan parasites of fish, crustaceans, and amphibians, and LKM51 exhibited a very high level of similarity (97.6%) with the "Prototheca richardsi" sequence. The latter sequence is from an agent that inhibits growth of amphibian larvae and was previously classified as a unicellular unpigmented alga (5). "P. richardsi" is the only DRIPs species whose free-living stage has been observed. None of the other DRIPs members have been cultured in vivo, and therefore the complete life histories of these parasites remain unclear. Apparently, our culture method supported growth of a free-living DRIPs species and may be used for further investigations of the life histories of these protistan parasites. Since we did not identify the eukaryotic microorganisms by microscopy, we have no information concerning the morphology of the species represented by sequence LKM51.
The resistance of most microbial species to cultivation has hampered the study of microbial community structure for many years. Recently introduced molecular techniques circumvent the problems and have been used increasingly to solve problems in microbial ecology. rDNA sequence information is now starting to reveal patterns and governing forces in natural microbial community structure (24). We used DGGE of the 16S and 18S rRNA genes to show that the origin of detritus can be a governing force in microbial community structure. Although many workers have used detritus as a natural carbon source to investigate bacterial production (17, 19, 39), to our knowledge we are the first researchers to describe detritus-dependent development of the microbial loop. Even though our study should be considered a survey and was carried out in small-scaled continuous-flow systems, the occurrence and subsequent decline of single-species phytoplankton blooms (2,20) may result in organic carbon releases comparable to carbon releases in our flow system. The effects described above can be expected to take place in natural systems.
We thank P. Schouten, H. Uittenhout, and G. M. van Hannen for construction of continuous culture boxes and UV-C devices and T. Beebe and M. Ragan for sharing the "P. richardsi" sequence before publication.
Received 9 December 1998.
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Applied and Environmental Microbiology Jun 1999, 65 (6) 2478-2484; DOI:
You are going to email the following Detritus-Dependent Development of the Microbial Community in an Experimental System: Qualitative Analysis by Denaturing Gradient Gel Electrophoresis


Mark Tansey (1949-). 1984, the Triumph of the New York School. Photo: Museum Kurhaus Kleve.
One of the most famous paintings of the Spanish painter Diego Velázquez is the Surrender of Breda. Velázquez painted the event in 1634-1635, ten years after it took place. The painting is a symbolic interpretation of the surrender of Breda in 1625, when Spanish troops defeated the beleaguered Dutch city. Two generals confront each other, while smoke comes over de heads of the defeated soldiers. On the right are the self conscious, well equipped Spanish troops. The general Justin of Nassau hands over the keys to the Spanish general Ambrogio de Spinola. The two generals meet each other, not in hate, on the contrary, Ambrogio comforts the general. A military drama becomes a human drama.
The painting inspired the artist Mark Tansey to symbolize the end of the battle between the Avantgarde artists from Europe and America. Old Europe of the 'École de Paris' and surrealists, represented by Picasso (with fur coat and just lightning a cigarette left from the table), Matisse (dressed like a WO I French general), Duchamp and André Breton, signing the surrender, have been beaten by American abstract expressionists.
The French army has a WO I outfit, lances and horses, symbolizing old Europe. The victorious American arts critic Clement Greenberg (who favoured American abstract expressionism) is seated behind the table with Jackson Pollock in the back, supported by a modern American Army with tanks. The New York School of abstract expressionists had won after a fierce but unequal battle. Appearances are deceptive however. The military victory may be clear cut, the future is far from secure for the American divisions. Their feet are standing in a swamp, the Europeans stay on solid ground. This painting also represents the difficult relations between French and American culture.


text:
Woman who faked disappearance faces sentence
A Michigan woman who faked her own disappearance and even put an ad on Craigslist to find an abductor is returning to court for her sentence.
Woman who faked disappearance faces sentence A Michigan woman who faked her own disappearance and even put an ad on Craigslist to find an abductor is returning to court for her sentence. Check out this story on battlecreekenquirer.com: http://bcene.ws/1uVHWYf
Associated Press Published 7:20 a.m. ET Nov. 20, 2014
Hayley Turner, 18, of Bedford Township, Mich., could face charges after faking her abduction.(Photo: Monroe County Sheriff)
MONROE – A Michigan woman who faked her own disappearance and even put an ad on Craigslist to find an abductor is returning to court for her sentence.
Related: Prosecutors charge woman who claimed abduction
Hayley Turner pleaded guilty to causing a false police report to be filed. She's returning to court Thursday in Monroe County.
Related: Ecorse police say missing Monroe County teen found alive
The 18-year-old Turner claimed she was abducted Aug. 7 in Bedford Township, just north of the Ohio-Michigan border. She was found safe with a puppy, 16 hours later in Ecorse, near Detroit.
As part of her plea deal, Turner must pay $15,000 to the sheriff's office for the search.
Read or Share this story: http://bcene.ws/1uVHWYf


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					buffer[n] = c
					get()
				end
			end

			if n > 0 then
				outN = outN + 1
				out[outN] = { tag = "string", table.concat(buffer) }
			end

			eatWhitespace()

			if outN == 0 then
				return { tag = "string", "" }
			elseif outN == 1 then
				return out[1]
			else
				return out
			end
		elseif c == "$" then
			local token = dollar()
			eatWhitespace()
			return token
		else
			local start = pointer

			while not symbols[c] and c ~= "\n" and c ~= "\t" and c ~= " " and c ~= "" do
				get()
				c = peek()
			end

			if start == pointer then
				generateError("Expected " .. name)
			end

			local str = text:sub(start, pointer - 1)
			eatWhitespace()

			if keywords[str] then
				return { tag = "keyword", str }
			else
				return { tag = "string", str }
			end
		end
	end

	local function parsePipe(current)
		if consume(">>") then
			return { tag = "append", current, string(stringSymbols, "filename") }
		elseif consume(">") then
			return { tag = "write", current, string(stringSymbols, "filename") }
		elseif consume("||") then
			return { tag = "or", current, expression() }
		elseif consume("&&") then
			return { tag = "and", current, expression() }
		elseif consume("|") then
			return { tag = "pipe", current, expression() }
		else
			generateError("Expected pipe")
		end
	end

	expression = function()
		if consume("(") then
			local cmd = command()
			expect(')')

			local c = peek()
			if c == "" or terminators[c] then
				return cmd
			elseif pipe[c] then
				return parsePipe(current)
			else
				return cmd
			end
		else
			return command()
		end
	end

	command = function(initial)
		if not initial then
			initial = string(commandSymbols, "command")
			if initial.tag == "keyword" then
				generateError("Unexpected keyword", 0)
			end
		end

		local current = { tag = "command", initial }
		local n = 1

		while true do
			local c = peek()
			if c == "" or terminators[c] then
				break
			elseif pipe[c] then
				return parsePipe(current)
			end

			n = n + 1
			current[n] = string(stringSymbols, "argument")
		end

		return current
	end

	local function block()
		local block, n = {}, 0

		local c = peek()
		if c == '' or c == '}' then
			return block
		end

		while true do
			n = n + 1
			block[n] = statement()

			local c = peek()
			if c == '' or c == '}' then
				break
			elseif c == ';' then
				get()
				eatWhitespace()
			else
				generateError("Expected ;")
			end

			-- Allow trailing ; on block
			c = peek()
			if c == '' or c == '}' then break end
		end

		return block
	end

	statement = function()
		if peek() == "(" then return expression() end

		local first = string(commandSymbols, "command")

		if first.tag == "keyword" then
			local keyword = first[1]

			if keyword == "if" then
				local conditional = expression()
				expect("{")
				local body = block()
				expect("}")

				local tag, n = { tag = "if", conditional, body }, 2

				while true do
					if consume("else") then
						expect("{")
						local body = block()
						expect("}")

						n = n + 1
						tag[n] = { tag = "else", body }

						break
					elseif consume("elseif") then
						local conditional = expression()
						expect("{")
						local body = block()
						expect("}")

						n = n + 1
						tag[n] = { tag = "elseif", conditional, body }
					else
						break
					end
				end

				return tag
			elseif keyword == "while" then
				local command = expression()
				expect("{")
				local body = block()
				expect("}")

				return { tag = "while", command, body }
			elseif keyword == "for" then
				local var = string(commandSymbols, "variable")
				expect("=")
				local command = expression()
				expect("{")
				local body = block()
				expect("}")

				return { tag = "for", var, command, body }
			else
				print("Unexpected keyword " .. keyword)
			end
		elseif consume("=") then
			return {
				tag = "set",
				first,
				string(stringSymbols, "value"),
			}
		else
			return command(first)
		end
	end

	eatWhitespace()
	local body = block()
	eatWhitespace()

	if pointer < #text then
		generateError("Expected EOF")
	end

	return body
end

redpajama_set_name: RedPajamaGithub


Giuseppe Zanotti "Natalie" glitter Lurex® fabric sneakers.
Swarovski® crystal embellished star appliqués.
Logo pull-tab at back collar.
Golden Goose sneakers in leather and glitter fabric. Signature star at side. 1" distressed heel. Round toe. Shimmery lace-up vamp. Logo at tongue and backstay. Padded footbed. Breathable leather/cotton lining. Signature stained rubber outsole. "Ball" is handmade in Italy.
Golden Goose crackled metallic leather sneakers with signature suede star at side. Chunky high platform. Round toe. Lace-up vamp. Padded footbed. Logo text at midsole. Breathable leather/cotton lining. Rubber outsole. Handmade in Italy.


You will be responsible for developing customisations in Dynamics 365 / CRM for a leading financial organisation.
My client is a leader in their sector and can offer an excellent working environment.
This is a great opportunity to work for a end user with a possibility of an extension in the new year.


text:
City of Regina Invests in Drainage Project
The City of Regina is investing $15 million in the North Central Drainage Project. The project will replace and upgrade the storm sewer system to help prevent ponding and flooding during large storms.
"North Central Regina has been identified as an area in our community that requires stormwater upgrades to reduce the risk of basement and street flooding," said Mayor Michael Fougere. "This major drainage project will benefit over 450 homes within the neighbourhood."
This project will reduce ponding impacts along Athol Street and along 2nd Avenue. Surface restorations will reduce the number of potholes to ensure rainwater is able to flow to the drains and catch basins.
To reduce the impact to residents and traffic, work will be completed in phases. In July 2020, as a part of phase one, work started along the North Storm Channel working south through Pony Park and crossing under the CN rail tracks.
The North Central Drainage Project is a three-year project that will be completed in 2022, pending weather or unforeseen circumstances. The project will include work on approximately 24 blocks within the area. A third construction season has been added due to impacts related to the COVID-19 pandemic.
Image Credit: City of Regina.
The health and safety of employees, crews, and community are top priority for the City of Regina. It is working closely with contractors and crews to ensure pandemic protocols are in place so work can be carried out in a safe and timely fashion.
In addition to the investment in the North Central Drainage Project, the City of Regina is investing $79.5 million for transportation, water, wastewater, and drainage projects this 2020. The investments will provide an important boost to the economy and help create jobs as the community deals with the economic impact of the COVID-19 pandemic.
More information about the City's construction projects planned for 2020 is available here.
Michael Fougere
Previous articleWater Has Crucial Role in Modernized Land Use Planning: POLIS
Next articleNova Scotia Provides Funding to Support Municipal Infrastructure
Top five stormwater stories of 2021
Alberta issues new guidelines for dealing with wastewater and stormwater
Warmer, wetter, wilder: 38 million people in the Great Lakes region are threatened by climate change
Green roofs help Canadian cities adapt to climate change
Saskatoon and USask study rubber tire-derived chemicals in stormwater
Budget 2021: A Healthy Environment for a Healthy Economy


text:
10 predictions for the 2020 NFL offseason
NFLBy Brad Gagnon on February 7, 2020 February 7, 2020
The 2020 NFL offseason is here. And with free agency just six weeks out and the draft in just over two months, it's time to make some predictions.
Here are 10 developments we're anticipating in the weeks and months to come:
1. Tom Brady signs with the Tampa Bay Buccaneers
It makes no sense for the Patriots to bring back Brady without a discounted rate, because that would prevent them from being able to re-sign all of their in-house free agents and also upgrade the rest of the offense. It's a lose-lose, and they'd be better off letting Brady walk. The Buccaneers aren't the favored landing spot, but they have the money to spend, they have Bruce Arians, and Brady would have the best 1-2 wide receiver duo in the NFL. I also think Arians would prefer Brady bridge the gap to a new quarterback in a year or two, rather than waiting out the interception-plagued Jameis Winston.
2. The New England Patriots don't replace Brady
The Pats could try to land somebody like Winston, Philip Rivers, Ryan Tannehill, Marcus Mariota, Andy Dalton, or Teddy Bridgewater. Or they could decide to save their money, regroup for a year, and tank for presumed No. 1 2021 draft pick Trevor Lawrence. I wouldn't be surprised if they did exactly that by starting Jarrett Stidham for a season.
3. Drew Brees returns to the New Orleans Saints for one more year
Ultimately, Brees will decide that there's too much talent and too much money is on the table to walk away after his highest-rated season yet. The 41-year-old still has it, and he's a competitor. He'll want to take one last shot at a second ring.
4. The Carolina Panthers trade Cam Newton to the Los Angeles Chargers
The Carolina Panthers are clearly entering a rebuild, so it doesn't make much sense to pay Newton more than $20 million in a walk year. They can save almost all of that by trading him away, and the Chargers make a lot of sense now that it's obvious Rivers is done in L.A. They're moving into a new stadium with the Rams, and this would be a hell of a way to drum up hype. Say what you will about Newton's game in recent years, but the guy is a brand.
5. Patrick Mahomes becomes the first $40-million-a-year player in NFL history
Russell Wilson is the current leader of the pack with an average annual salary of $35 million. But it's a year later now, and Mahomes is both better and younger. You have to figure that's worth at least $38 million per year, but likely closer to $40 million. I figure that in the midst of all the euphoria, the Chiefs will just throw him an even $200 million over five years. The alternative is to wait a year, but Mahomes could become a lot more expensive in 2021 or 2022.
6. Dak Prescott gets stuck with the franchise tag
Prescott was willing to bet on himself in 2019, and if Mahomes strikes a deal like that, I'm guessing he wouldn't be willing to accept anything less than $35 million per year from the Dallas Cowboys. He'll bet on himself one more time, but in this case at a rate of about $27 million for 2020.
7. Both the Cincinnati Bengals and Washington Redskins stay put and draft Joe Burrow and Chase Young, respectively
Burrow could be a generational talent at quarterback, which is exactly what the Bengals need. Young could be a generational talent on defense, which is exactly what the Redskins need. And so for the third year in a row, the top two picks won't be traded.
8. The Miami Dolphins draft Tua Tagovailoa
That would enable the draft-pick-rich Dolphins to navigate the top 10 in order to land the 2018 SEC player of the year. Why the hell not? Might as well load up on as many potential franchise quarterbacks as possible, and with the No. 5 overall pick, moving up a spot or two shouldn't be too prohibitive anyway. Plus, they can wait for Tua's hip to get right.
9. Tony Romo returns to CBS, Jay Cutler joins Monday Night Football
Andrew Marchand of the New York Post reported recently that CBS "is prepared to compete very aggressively" to keep Romo, who everyone knows will command a massive payday this offseason. I think ESPN will court him for Monday Night Football but lose out due to CBS' right of first refusal. But the MNF is so product that the desperate Worldwide Leader will instead take a shot at Cutler, who has reportedly been meeting with networks about a future in TV.
10. The regular season arrives without a new collective bargaining agreement
Deadlines work wonders, and we're not even close to that. Everything you're hearing now is strategic. Both sides are posturing, and I really don't think the players are down for 17 games. I'm not sure the owners expect the players to go for that anyway, but it's a bargaining chip, and it will probably linger well into 2021.
cam newtondrew breeslos angeles chargersNew England PatriotsTampa Bay BuccaneersTom Brady
About Brad Gagnon
Brad Gagnon has been passionate about both sports and mass media since he was in diapers -- a passion that won't die until he's in them again. Based in Toronto, he's worked as a national NFL blog editor at theScore.com, a producer and writer at theScore Television Network and a host, reporter and play-by-play voice at Rogers TV. His work has also appeared at CBSSports.com, Deadspin, FoxSports.com, The Guardian, The Hockey News and elsewhere at Comeback Media, but his day gig has him covering the NFL nationally for Bleacher Report.
View all posts by Brad Gagnon	Follow on Twitter
Five NFL contracts that are about to really suck
Why we needed sports in 2020 more than ever
Remembering some of Daniel Tosh's best sports sketches as 'Tosh.0' wraps up its 12-year run
Why 'Jeopardy!' contestants tend to do so poorly with sports categories
Padres reportedly acquiring Joe Musgrove from Pirates, continuing their bold strategy of adding talented players
The Padres might not be better than the Dodgers, but at least they're trying to get there.
By Joe Lucia	On Jan 18, 2021 0
Crazy stats from the 2020 NFL season
The highlights and lowlights of the 2020 NFL regular season


Artificial Intelligence is far more than just machine learning. There are a variety of methods that can be used to automate decision making. In this introductory course for software developers, we will explore various methods. The goal is to give devs newer to the field a few "eureka moments" during a survey of AI methodologies. Experienced devs will gain a fresh perspective on the field of AI from a fellow developer, not a mathematician.
Barry is a .NET Software Engineer who has been creating enterprise solutions for more than 30 years. Barry is also an Election Integrity Activist, baseball and hockey fan, husband of one genius and father of another, and a 30+ year resident of Phoenix Arizona USA. Barry spends his days building intelligent systems for enterprise customers and his nights thinking about the next AZGiveCamp where software developers come together to build websites and apps for some great non-profit organizations.


text:
echo "Building node-webkit..."

if [ "$KEY_PASSWORD" == "" ]; then
  echo "KEY_PASSWORD is blank"
  exit 1
fi

BUILD_DIR=`pwd`
NODE_WEBKIT_VERSION=0.11.5
ulimit -n 8192

rm -rf build

echo "Cloning gateblu-ui..."
rm -rf gateblu-ui
git clone git://github.com/octoblu/gateblu-ui
cd gateblu-ui
npm install
node_modules/.bin/gulp
cd $BUILD_DIR

echo "Building node-webkit..."
npm install
node build
node build # needs to be run twice

rm -rf $BUILD_DIR/packages
mkdir -p $BUILD_DIR/packages

echo "Building Mac..."
cd $BUILD_DIR
cd build/Gateblu/osx64/Gateblu.app/Contents/Resources/app.nw
mkdir dist
tar zxf $BUILD_DIR/dist/node-v0.10.35-darwin-x64.tar.gz --directory dist/

echo "Creating Mac Installer..."
cd $BUILD_DIR
sh installer-osx.sh

echo "Building Windows..."
cd $BUILD_DIR
cd build/Gateblu/win32
WIN_DIR=dist/node-v0.10.35-win-x86/bin
mkdir -p $WIN_DIR/node_modules
cp $BUILD_DIR/dist/node-v0.10.35-win-x86/node.exe $WIN_DIR
unzip -q $BUILD_DIR/dist/node-v0.10.35-win-x86/npm-2.6.0.zip -d $WIN_DIR
mv $WIN_DIR/npm-2.6.0 $WIN_DIR/node_modules/npm
cp $WIN_DIR/node_modules/npm/bin/npm.cmd $WIN_DIR
mv Gateblu.exe package.nw
cp $BUILD_DIR/cache/$NODE_WEBKIT_VERSION/win32/nw.exe Gateblu.exe

echo "Creating Windows Installer..."
cd $BUILD_DIR
sh installer-win.sh

echo "Building Linux 32-bit..."
cd $BUILD_DIR
cd build/Gateblu/linux32
mkdir dist
tar zxf $BUILD_DIR/dist/node-v0.10.35-linux-x86.tar.gz --directory dist/
LC_CTYPE=C && LANG=C && sed -i '' 's/udev\.so\.0/udev.so.1/g' Gateblu

echo "Building Linux 64-bit..."
cd $BUILD_DIR
cd build/Gateblu/linux64
mkdir dist
tar zxf $BUILD_DIR/dist/node-v0.10.35-linux-x64.tar.gz --directory dist/
LC_CTYPE=C && LANG=C && sed -i '' 's/udev\.so\.0/udev.so.1/g' Gateblu

echo "Creating Linux Installer..."
cd $BUILD_DIR
sh installer-linux.sh


text:
<?php

namespace App\Http\Controllers;

use Illuminate\Http\Request;

use App\Http\Requests;
use Validator;
use Input;
use Redirect;
use Auth;
use App\Feedback_report;
use Illuminate\Database\Seeder;
use Illuminate\Database\Eloquent\Model;
use Hash;

class FeedbackController extends Controller
{
    public function returnPage(){

        	return view('pages/feedback');

        }

     //Report bug function
	public function giveFeedback(){

		$input	=	Input::all();												//retrieves all input from the users form
		$rules	=	array(	 													//change the table name once the updatedd database base has been created
						'feedbackTitle'  		=> 	'required', 		//this field is required as it is in the errors table
						'feedbackDescription' 			=> 	'required',
					 	);

    	$v = Validator::make($input, $rules);									//this acts as a boolean

		if($v->passes()){
														//if the form meets all the requirements

			$feedback = new Feedback_report; 										//creating an object which is going to store a record of data using the save() function
			$feedback->feedback_subject 			= $input['feedbackTitle'];
			$feedback->feedback_report				= $input['feedbackDescription'];
			$feedback->user_id = Auth::user()->id;
			$feedback->save(); 													//the save function insets a record into the database

			return Redirect::to('feedback');

		}else{

			return Redirect::to('feedback')->withInput()->withErrors($v); 	//returns the user to the 'errors page' with the error specified

		}

	}
}


5 days ago Tips Sign Up real name is Sean Combs, is dating Instagram model Jocelyn Chew problem showing the Bad Boy Records founder what he's missing. Text. Color. White, Black, Red, Green, Blue, Yellow, Magenta, Cyan . Billionaire soccer team owner's helicopter crashes moments after leaving match Here's a few simple steps to help create a retro-style text effect. Tips on how you can generate more Add age, texture and depth to your . will match your vintage design Discover and customize the font Vintage Punk and other Landscape: NEON TEXT GENERATOR | CHALKBOARD GENERATOR Keep up to date with free dating chat usa Match.com dating tips for guys texting Im texting with a guy who is a Rushed Relationships: Signs, He may very well the guy who Home » Dating Advice Blog » The Dating with Dignity Point of View . up for a FREE 21-day trial to He by doing this is just testing the waters. match. Ok, I'm not an expert on dating advice, and I don't really have the holy grail on the best online dating profile examples for men and paired them with quick tips on easier to get naked pictures and dirty texts from girls you've slept with before. This happens right after he Should you text a guy after a date is a commonly .. Tagged on: a little nudge dating advice erika ettin online dating and texting pase meetic 3 dias gratis venezuela Match.com dating tips for guys texting The Ultimate Guide to Decoding Guys Lisa Winning, Carrie Henderson McDermott The back-and-forth text volley is as blis- tering as a Serena Williams match. ✽✽ A funny reply is always at the tips of your fingers. ✽✽ The tone is light. too into you. ✽✽ The texts have a purpose, namely, making plans for your next date.
We surveyed 50,000 guys about their online dating expereince and less than Use a few of these tips for how to keep a text conversation going next time to Jun 13, 2015 I frequently talk about them on my dating women radio show but like anything I don't recommend you do this I teach guys that texting, instant tener feeling con alguien definicion Match.com dating tips for guys texting Our resident agony aunt, dating expert Charly Lester, offers advice on what to do when someone Select One, man, woman . A match and I were exchanging messages but it's now been a week since we've been in contact. they're messaging you – more like you would behave if one your friends were to send you a text.
What Happens When You Meet Up With A Guy You Already Know On Tinder. Investigating Mobile Dating Applications in the Tinder Age. you as a potential match, then you are able to start chatting Check out our tips and tricks below even if you take a screenshot at the end. sharing a screenshot of a text from Aponte. encuentra amigos gratis hoy Match.com dating tips for guys texting The daughter is dating "Superjew" and mom is constantly sending her texts on how to 0 world, dating texting rules, Julie Spira, online dating expert, relationship advice, texting . 11 'Silly & Funny' Texts To Make Your Guy Laugh Like Crazy!!
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Is it considered poor etiquette to not respond to messages that a guy obviously put Any other general tips would be good. . When I was doing online dating, I would sometimes reply with a "Sorry, not interested, good luck! to be. if you go home with someone, text their address to a friend. i used the "yo" View: a great online dating advice for men - dating in asia an era of romance tips for Texting things i ve ever got a modern nerd dating advice and guys? hegel frases dificiles Match.com dating tips for guys texting She understands we had a rebound relationship as the guy she went out with before But after only a few texts or a Facts About Rebound Relationships My ex came . Dating Tips - Match. com/wp-content/uploads/2015/01/Unfriend-your-ex. Jan 19, 2016 As online dating coach Patrick King explains, they've already given you Texting the cute guy from the gym when he's trying to sleep will turn This was by far the most common advice you'll find: don't just text someone "hey.


Is there such thing as darkness?
This question reflects one of the earliest and most divisive disputes in philosophy: the existence of universals. When we conduct an inventory of the things in the universe we will find red things, tall things, just things(/people), courageous things(/people), but will we find in addition to those things also redness, tallness, justice, and courage.
Plato and Aristotle are the best known proponents of each side of this dispute, with Plato taking the side of those who believe in universals (also known as platonic forms), and Aristotle taking the side of those who deny the existence of universals.
Plato's main argument (though he doesn't seem to feel the need for much of one) is that we could not perceive things as having any common characteristics unless we were acquainted with the relevant universal.
Aristotle's bets known argument is the third man argument. If two things are both men, and resemble each other in this respect only because they both instantiate the universal MAN, then what of the universal itself? It would seem that it could only resemble particular men if there was another universal (a third man) which they all instantiated. If not and the universal and the particular men can resemble each other without participating in higher universal, then surely the original individuals can resemble each other (and all be men) without the need of a universal MAN at all.
In Aristotle's argument he relies on the assumption that universals are true of themselves: that the TALL will itslef be tall, that COURAGE will itself be courageous and so on. To modern eyes this seems ridiculous, but it was accepted by the vast majority of Ancient Greek philosophers. Still, the relationship of instantiation itself seems to need a universal (if any relationship does at all) and so some form of the argument still seems good.
On the skeptical side no one has a workable account of when two universals are the same. Merely being true of the same things isn't enough (consider creature with a heart, and creature with a kidney, these two properties are surely different, everything with a heart has kidneys and vice versa).
On the positive side there seem to be some statements (such as "Humility is a virtue") that seem to unavoidably rely upon the existence of universals. Those against universlas must either give a plausible re-interpretation of such claims which doesn't rely upon universals, or abandon such statements altogether.
Putting aside the general issue of universals and looking at darkness are there any reason to suppose DARKNESS is particularly implausible?
Yes, it is both subjective and seemingly inessential to our claims about the world. Not only is darkness relative to particular visual equipment (what is dark to you ro I is not dark to an owl or a frog), and hence it would seem not a key feature of the worlds functioning. Moreover it would seem we could replace talk of DARKNESS with talk of 'receiving insufficient light to form a visual image'. This might still leave us with a universal to deal with but it wouldn't be DARKNESS. More generally we seem to have perfectly adequate and successful theories of the world which don'f use the term darkness at all. We could surely paraphrase claims about darkness in terms of average number of photons being received per square meter per second.
For my part I find (a modern version of) Aristotle's argument pretty convincing, and I don't believe in universals at all.
Nathan Sinclair recently joined the Board of the ISFP. He has a PhD in Philosophy from the University of Sydney.
Next ›Aren't we happier not knowing that our 'real' world isn't real?
So then how come we continue to use common nouns? How can we use language and logic at all, since both depend on there actually being universals.
Also, Aristotle was a realist, but a moderate one. He did believe in universals in the form of essences, which the mind instantiates from particulars.


text:
Behind The Desk 127: Last Night I Saw A Band
Cute StuffMusic 04 min read
Brandon Wetherbee is the host of the talk show You, Me, Them, Everybody. He'll be hosting YMTE at the Wonderland Ballroom in D.C. tonight with guests Matt Cohen, Laura Meagher, Katie McD, Natalie McGill, Jenn Tisdale and more.
Last night I saw a band that reminded me why I do what I do. Today I listened to a lecture that reminded me why I do what I do. These reminders will get me through the next year, even if nothing new and good happens.
The previous 24 hours has taken me back to when I was a teenager, when hormones screwed up brain chemistry in a way only a rational adult can see. Hindsight, etc. Last night and earlier today I felt like a kid in an ugly basement, making ugly sounds, writing dumb but VERY IMPORTANT thoughts and feelings about what MATTERS. All caps necessary.
Landlady, a band I've written about more than any other band in 2014, played Black Cat's Backstage to a warm, receptive and happy crowd. I will not apologize for my praise and friendship with this band. I found out about the band thanks to D.C.'s biggest D.I.Y. supporter Alex Tebeleff. I was looking for bands for a YMTE at SXSW and Alex recommended this 6-piece (occasional 5-piece, occasional 4-piece). Since I trust Alex, I booked the band. Now I will always trust Alex. Landlady put on one of the best sets I've ever seen. To a very intimate crowd. Last night wasn't much different. Thankfully, the crowd wasn't as intimate.
This has only happened three times, I see a band I like on record and fall in love with live. The other two are Detachment Kit and sally. Most likely you have not heard of them. That's a shame and something I take as a defeat. That's why I do what I do. Spreading the love of a band or artist doesn't sound very important and it's not (we're not curing any diseases, I'm not just beating myself up for sympathy), but it has the potential to make my world, and the world at large, smaller. If that's possible, we're better off. (I wrote that last sentence while standing in the corner of the Black Cat while listening to my favorite working band so I'm music high. Forgive me.)
Landlady triggers memories before the song was recorded. That's hard. Their songs and Adam's live performance, equal parts David Byrne and Wayne Coyne, take me to overly romanticized, potentially made up memories of happiness. I'd like to clarify I'm writing this completely sober.
The following paragraph was also written during the concert:
I don't want to be writing this, I want to enjoy the show, but I don't want to forget. I want to be in the moment. I want to spread the love for this band. Unless you have a child, you should be here. This is the kind of band that makes me want to write a zine. That's rare. That's special and should be cherished.
I wasn't going to publish this because it's so flighty and saccharine. Then I listened to Iggy Pop's John Peel Lecture and am embracing it. Embracing that feeling that made the music feel important and joyful and worth spreading.
Iggy is a man I've written about. Iggy is a man I tried to get officiate my wedding. Iggy is a man I've followed and hopefully taught me and every other performers/musician/writer/artist/person something. Today I learned that music can have a value that can only be judged in feeling. Watch this video. It should make you feel something.
Iggy's lecture is getting some social media traction because he spoke about U2's new, free album. That's not why I'm recommending listening to the hour long speech. I'm recommending it because it will hopefully remind you why you liked music in the first place. Regardless of crooks and pirates and all that dirty stuff that's been around since music, it's still worth loving.
Landlady's Adam Schatz and Iggy Pop are very different men. One man drinks tea on stage. One man battled addiction and bled on stage. But they're also very similar. Both love indie labels. Both give one hell of a live show. Both are extremely important. Both helped remind me why I do what I do. Give them both a listen.
Download and stream Iggy Pop's John Peel Lecture.
Purchase Landlady's new album Upright Behavior. See them. They're on tour. They're always on tour.
iggy pop John PeelLandladypaperhausThe Stooges
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With the focus on discussing the role of private sector in sustainable development financing and the opportunities in creating new partnerships, the aim of this event is to provide a substantive insight for the work of the Committee of Experts on Sustainable Development Financing (ICESDF).
Based on the Monterrey concept of comprehensive development finance, the private flows, especially trade and investments, play a key role in development. New kinds of partnerships are needed, where poverty eradication and sustainable development in its economic, social and environmental aspects are fully considered.
The aim of this event is to bring together a wide range of stakeholders, with different backgrounds, to discuss the state of play of the partnerships today and to work together to innovate opportunities on mutually beneficial cooperation that may be co-created among stakeholders. The program for the 1,5-day event will be interactive and participatory.
The outcome of the event will be provided for the consideration of the ICESDF. In addition the outcome will be provided for information to the discussion on the role of partnerships in the implementation of the Post-2015 development agenda, organized by the President of the General Assembly in New York on 9-10 April 2014.


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New Restaurant "Cuts" into Truva Downtown
CUTs is coming soon to downtown Atlanta.
A new restaurant called CUTs Steakhouse plans to open later this week in downtown Atlanta. CUTs is being opened by the same owners as Truva, a Mediterranean eatery that opened in late 2009. When Truva opened, it occupied the lower level of a two level restaurant with scarcely used banquet space occupying the upper floor. After renovating the upstairs, Truva moved upstairs and after now renovating the downstairs, CUTs is slated to open this coming Friday, January 3rd.
The entrance to Truva will now be off Peachtree Center Avenue, while the entrance to CUTs will be the former Truva entrance on Andrew Young International Drive.
CUTs joins a competitive downtown restaurant scene that has in recent years welcomed both White Oak Kitchen & Cocktails as well as Alma Cocina, both popular restaurants with local operators. As far as steakhouses go, downtown is not nearly as competitive as Buckhead. Steakhouse options downtown are basically Ruth's Chris or Morton's, and on the more casual side, Durango Steakhouse on Peachtree Street. Buckhead, on the other hand, offers local options such as Chop's, Hal's, Bone's, Prime and New York Prime in addition to chain outlets Ocean Prime, The Capital Grille, Ruth's Chris, Davio's, Del Frisco's Grille and The Palm.
The Truva / Cuts space, located at 60 Andrew Young International Boulevard was originally built as a Steak & Ale.
Labels: Eateries by Atlantan99
I used to love that Steak and Ale! When they first opened the service was impeccable and the food was perfect. The salad bar was a real gourmet treat back then! The last time I ate there was about a month before they closed and even though the closing was a surprise, it was time for an end. A waitress that worked there was basically opening her blouse for you to see her breasts up close for an extra tip. She was well endowed and I'm sure well paid.
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