text:
Time to reflect and celebrate
Are women in India feeling more "empowered" today, asks Kalpana Sharma on International Women's day.
March 8 is International Women's Day. Yet again we will go through the tokenism of remembering that one half of our population — a declining half — consists of women. So is this half of the population better off? Are women in India feeling more "empowered" today, in an India that some claim is "shining", than they did two decades back? Can Indian women dream of a day when they will be treated with the same respect and rights as men, in fact as all human beings, men and women, should?
International Women's Day — and the very fact that there has to be such a day — is an occasion to celebrate and reflect. We can celebrate the fact that some things have changed because of the relentless campaigning and struggles by women's groups in India and worldwide. And we can sit back and reflect on the long and rocky road that still lies ahead for the majority of women before they can be confident, secure and safe.
But first let us look at the "celebrate" part. What can we celebrate? (Given below is a rough and ready list. I am sure readers will have many more suggestions that they can send to me.)
• That despite efforts to kill them off before they are born, and after they are born, girls in India are surviving and growing up into confident young women.
• That more girls are now going to school than in 1984 —with many of them topping board examinations.
• That the women's hockey team won the Asia Cup despite no support, no sponsorship and pathetic living conditions compared to their male counterparts.
• That the women's cricket team now has a sponsor — even it if is a stereotypical women's product, jewellery, small change compared to the level of support for the men's team.
• That women entrepreneurs and managers are making their mark in a world of men, even if recognition comes by way of separate women's awards.
• That despite the obduracy of male politicians with regard to the Women's Bill in Parliament, more women are visible in the political spectrum and in the run up to the forthcoming general elections.
But even as we celebrate all this, we must pause, reflect and ask.
• Why the numbers of women as compared to men continue to decline in India?
• Why despite changes in the rape laws, the convictions for rape are few and far in between while the incidence of violent crimes continues to grow?
• Why despite the Supreme Court ruling in the Vishakha case on sexual harassment there is little change in the reality on the ground with a majority of women being unaware of their rights and the majority of men blithely confident that they can get away with it?
• Why despite the fact that India has the largest number of professionally qualified women in the world do parents continue to sell their daughters into marriage, often miserably unhappy ones, by paying unaffordable amounts as dowry?
• Why despite progress in science and medicine, millions of Indian women die each year giving birth?
• Why despite mountains of words about women's right to participate as equals in politics, the Bill to reserve one-third of the seats in Parliament for women dies an unnatural death in successive Lok Sabhas, unable to be introduced, leave alone discussed or passed?
Fortunately, despite the depressing answers to these questions, there is a glimmer of hope. One sees it when you listen to the views of girls like the daughter of a taxi driver in Mumbai who lives in one of the typical "chawls" (one-room tenements) that dot the city. She spoke in English to a television channel recently and confidently stated her desire to join the media when she finished her studies. Why, she was asked. "Because it will give me a chance to meet so many different types of people," she said.
We can celebrate the fact that some things have changed because of the relentless campaigning and struggles by women's groups. And we can sit back and reflect on the long and rocky road that still lies ahead.
If girls like this daughter of a Mumbai taxi driver can dream, and have the confidence to share their dreams before a television camera, then there is reason to have at least half a celebration. For it suggests that even as things get worse for many women in the country — as the gap between the rich and the poor grows, as levels of poverty stay static or increase, as water and fuel become scarce, as survival continues to mean the ability to find enough for one square meal a day, as they continue to be victims of violence of the feuds perpetuated by their men — they have improved for some others.
Yet, the glow from these stories of success, of girls and women who have overcome obstacles to achieve something, should not hide the continuing areas of darkness. The "feel good" motto of the ruling coalition has rubbed off on the media. There is a lot of writing about the achievements of individuals, including women, and institutions. Yet, you hear little about the women away from the media glare, for whom things are not going so well. As a result, it is easy to build an illusionary world, one that highlights the successes without balancing them with failures.
On March 8, we have to look at both. It is only if we have a real sense of what is happening with women, all women, rich and poor, young and old, can we hope to arrive at strategies and solutions that can make a difference and go beyond rhetoric and tokenism. "Real women" have no problem dealing with both — the celebration and the reflection.
Kalpana Sharma is Deputy Editor with The Hindu, and a regular contributor to India Together. Her opinions, which appear in a regular column with The Hindu, are concurrently published on India Together with permission.
http://indiatogether.org/8womday-op-ed


text:
package org.apache.skywalking.apm.agent.core.plugin.bytebuddy;

import net.bytebuddy.description.method.MethodDescription;
import net.bytebuddy.matcher.ElementMatcher;
import org.junit.Assert;
import org.junit.Test;

public class ReturnTypeNameMatchTest {

    @Test
    public void testMatch() throws Exception {
        final ElementMatcher<MethodDescription> matcher = ReturnTypeNameMatch.returnsWithType("java.lang.String");
        Assert.assertTrue(matcher.matches(new MethodDescription.ForLoadedMethod(Person.class.getMethod("getName"))));
        Assert.assertFalse(matcher.matches(new MethodDescription.ForLoadedMethod(Person.class.getMethod("getAge"))));
    }

}

redpajama_set_name: RedPajamaGithub


text:
Pereira, O., et al. "Genomic ecology of Marine Group II, the most common marine planktonic Archaea across the surface ocean." MicrobiologyOpen. 8.9 (2019): e852.
Résumé: Planktonic Archaea have been detected in all the world's oceans and are found from surface waters to the deep sea. The two most common Archaea phyla are Thaumarchaeota and Euryarchaeota. Euryarchaeota are generally more common in surface waters, but very little is known about their ecology and their potential metabolisms. In this study, we explore the genomic ecology of the Marine Group II (MGII), the main marine planktonic Euryarchaeota, and test if it is composed of different ecologically relevant units. We re-analyzed Tara Oceans metagenomes from the photic layer and the deep ocean by annotating sequences against a custom MGII database and by mapping gene co-occurrences. Our data provide a global view of the distribution of Euryarchaeota, and more specifically of MGII subgroups, and reveal their association to a number of gene-coding sequences. In particular, we show that MGII proteorhodopsins were detected in both the surface and the deep chlorophyll maximum layer and that different clusters of these light harvesting proteins were present. Our approach helped describing the set of genes found together with specific MGII subgroups. We could thus define genomic environments that could theoretically describe ecologically meaningful units and the ecological niche that they occupy.
Mots-Clés: 16S rRNA; abundance; assemblages; bacteria; carbon; community; dynamics; euryarchaeota; Euryarchaeota; global ocean; insights; metagenomics; organisms; poseidoniales; proteorhodopsin
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Dalongeville, A., et al. "Combining six genome scan methods to detect candidate genes to salinity in the Mediterranean striped red mullet (Mullus surmuletus)." BMC Genomics. 19 (2018): 217.
Résumé: Background: Adaptive genomics may help predicting how a species will respond to future environmental changes. Genomic signatures of local adaptation in marine organisms are often driven by environmental selective agents impacting the physiology of organisms. With one of the highest salinity level, the Mediterranean Sea provides an excellent model to investigate adaptive genomic divergence underlying salinity adaptation. In the present study, we combined six genome scan methods to detect potential genomic signal of selection in the striped red mullet (Mullus surmuletus) populations distributed across a wide salinity gradient. We then blasted these outlier sequences on published fish genomic resources in order to identify relevant potential candidate genes for salinity adaptation in this species. Results: Altogether, the six genome scan methods found 173 outliers out of 1153 SNPs. Using a blast approach, we discovered four candidate SNPs belonging to three genes potentially implicated in adaptation of M. surmuletus to salinity. The allele frequency at one of these SNPs significantly increases with salinity independently from the effect of longitude. The gene associated to this SNP, SOCS2, encodes for an inhibitor of cytokine and has previously been shown to be expressed under osmotic pressure in other marine organisms. Additionally, our results showed that genome scan methods not correcting for spatial structure can still be an efficient strategy to detect potential footprints of selection, when the spatial and environmental variation are confounded, and then, correcting for spatial structure in a second step represents a conservative method. Conclusion: The present outcomes bring evidences of potential genomic footprint of selection, which suggest an adaptive response of M. surmuletus to salinity conditions in the Mediterranean Sea. Additional genomic data such as sequencing of a full-genome and transcriptome analyses of gene expression would provide new insights regarding the possibility that some striped red mullet populations are locally adapted to their saline environment.
Mots-Clés: Adaptive genomics; Candidate genes; climate; genetics; Genome scan; landscape genomics; local adaptation; Mediterranean Sea; methionine sulfoxide reductase; Mullus surmuletus; population genomics; principal component analysis; r package; Salinity; selection; threespine sticklebacks
Jacquemot, L., et al. "Therapeutic potential of a new jumbo phage that infects Vibrio coralliilyticus, a widespread coral pathogen." Front. Microbiol.. 9 (2018): 2501.
Résumé: Biological control using bacteriophages is a promising approach for mitigating the devastating effects of coral diseases. Several phages that infect Vibrio coralliilyticus, a widespread coral pathogen, have been isolated, suggesting that this bacterium is permissive to viral infection and is, therefore, a suitable candidate for treatment by phage therapy. In this study, we combined functional and genomic approaches to evaluate the therapeutic potential of BONAISHI, a novel V. coralliilyticus phage, which was isolated from the coral reef in Van Phong Bay (Vietnam). BONAISHI appears to be strictly lytic for several pathogenic strains of V. coralliilyticus and remains infectious over a broad range of environmental conditions. This candidate has an unusually large dsDNA genome (303 kb), with no genes that encode known toxins or implicated in lysogeny control. We identified several proteins involved in host lysis, which may offer an interesting alternative to the use of whole bacteriophages for controlling V. coralliilyticus. A preliminary therapy test showed that adding BONAISHI to an infected culture of Symbiodinium sp. cells reduced the impact of V. coralliilyticus on Symbiodinium sp. photosynthetic activity. This study showed that BONAISHI is able to mitigate V. coralliilyticus infections, making it a good candidate for phage therapy for coral disease.
Mots-Clés: bacterial pathogen; bacteriophages; coral disease; database; disease; phage therapy; phage-host interactions; phi-kz; ralstonia-solanacearum; reef ecosystems; sequence; transfer-rna genes; Vibrio coralliilyticus; viral genomics; virulence factors
Hillen, J. E. J., et al. "Estimates of genetic variability and inbreeding in experimentally selected populations of European sea bass." Aquaculture. 479 (2017): 742–749.
Résumé: The aquaculture industry has increasingly aimed at improving economically important traits like growth, feed efficiency and resistance to infections. Artificial selection represents an important window of opportunity to significantly improve production. However, the pitfall is that selection will reduce genetic diversity and increase inbreeding in the farmed stocks. Genetic tools are very useful in this context as they provide accurate measures of genetic diversity together with many additional insights in the stock status and the selection process. In this study we assessed the level of genetic variability and relatedness over several generations of two lines of experimentally selected European sea bass (Dicentrarchus labrax L.). The first line was selected for growth over three generations and the second line for both high and low weight loss under a starvation regime over two generations. We used a genomic approach (2549 single nucleotide polymorphism markers derived from double digest restriction site associated DNA sequencing) in combination with eight microsatellites to estimate genetic variation, relatedness, effective population size and genetic differentiation across generations. Individual heterozygosity estimates indicated that the selected lines showed no significant reduction in diversity compared with wild populations. There was, however, a decreasing trend in allelic richness, suggesting the loss of low frequency alleles. We compared the estimates of effective population size from genetic markers with pedigree information and found good correspondence between methods. This study provides important insights in the genetic consequences of selective breeding and demonstrates the operational use of the latest genomic tools to estimate variability, inbreeding and at a later stage domestication and artificial selection.
Mots-Clés: Artificial selection; ddRAD; fish; genetic diversity; Genomics; Inbreeding
Zarski, D., et al. "Transcriptomic Profiling of Egg Quality in Sea Bass (Dicentrarchus labrax) Sheds Light on Genes Involved in Ubiquitination and Translation." Mar. Biotechnol.. 19.1 (2017): 102–115.
Résumé: Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were used. We identified 39 differentially expressed genes exhibiting a differential expression between the groups of low (fertilization rate < 60 %) and high (fertilization rate > 60 %) quality. The mRNA levels of eight genes were further analyzed by quantitative PCR. Seven genes were confirmed by qPCR to be differentially expressed in eggs of low and high quality. This study confirmed the importance of some of the genes already reported to be potential molecular quality indicators (mainly rnf213 and irf7), but we also found new genes (mainly usp5, mem-prot, plec, cenpf), which had not yet been reported to be quality-dependent in fish. These results suggest the importance of genes involved in several important processes, such as protein ubiquitination, translation, DNA repair, and cell structure and architecture; these probably being the mechanisms that contribute to egg developmental competence in sea bass.
Mots-Clés: aquaculture; broodstock management; Controlled reproduction; expression; fish; gadus-morhua l.; genomics; indicator; lipid droplets; markers; Microarray; oncorhynchus-mykiss; rainbow-trout; reproduction; Transcriptomics
Pecoraro, C., et al. "Methodological assessment of 2b-RAD genotyping technique for population structure inferences in yellowfin tuna (Thunnus albacares)." Marine Genomics. 25 (2016): 43–48.
Résumé: Global population genetic structure of yellowfin tuna (Thunnus albacares) is still poorly understood despite its relevance for the tuna fishery industry. Low levels of genetic differentiation among oceans speak in favour of the existence of a single panmictic population worldwide of this highly migratory fish. However, recent studies indicated genetic structuring at a much smaller geographic scales than previously considered, pointing out that YFT population genetic structure has not been properly assessed so far. In this study, we demonstrated for the first time, the utility of 2b-RAD genotyping technique for investigating population genetic diversity and differentiation in high gene-flow species. Running de novo pipeline in Stacks, a total of 6772 high-quality genome-wide SNPs were identified across Atlantic, Indian and Pacific population samples representing all major distribution areas. Preliminary analyses showed shallow but significant population structure among oceans (FST = 0.0273; P-value < 0.01). Discriminant Analysis of Principal Components endorsed the presence of genetically discrete yellowfin tuna populations among three oceanic pools. Although such evidence needs to be corroborated by increasing sample size, these results showed the efficiency of this genotyping technique in assessing genetic divergence in a marine fish with high dispersal potential.
Mots-Clés: Marine fish; Population genomics; RAD sequencing; Snp; Tropical tuna; Tuna fishery
GAGNAIRE, P. - A., et al. "Using neutral, selected, and hitchhiker loci to assess connectivity of marine populations in the genomic era." Evolutionary Applications. 8.8 (2015): 769–786.
Résumé: Estimating the rate of exchange of individuals among populations is a central concern to evolutionary ecology and its applications to conservation and management. For instance, the efficiency of protected areas in sustaining locally endangered populations and ecosystems depends on reserve network connectivity. The population genetics theory offers a powerful framework for estimating dispersal distances and migration rates from molecular data. In the marine realm, however, decades of molecular studies have met limited success in inferring genetic connectivity, due to the frequent lack of spatial genetic structure in species exhibiting high fecundity and dispersal capabilities. This is especially true within biogeographic regions bounded by well-known hotspots of genetic differentiation. Here, we provide an overview of the current methods for estimating genetic connectivity using molecular markers and propose several directions for improving existing approaches using large population genomic datasets. We highlight several issues that limit the effectiveness of methods based on neutral markers when there is virtually no genetic differentiation among samples. We then focus on alternative methods based on markers influenced by selection. Although some of these methodologies are still underexplored, our aim was to stimulate new research to test how broadly they are applicable to nonmodel marine species. We argue that the increased ability to apply the concepts of cline analyses will improve dispersal inferences across physical and ecological barriers that reduce connectivity locally. We finally present how neutral markers hitchhiking with selected loci can also provide information about connectivity patterns within apparently well-mixed biogeographic regions. We contend that one of the most promising applications of population genomics is the use of outlier loci to delineate relevant conservation units and related eco-geographic features across which connectivity can be measured.
Mots-Clés: connectivity; gene flow; marine conservation; population genomics; Population structure

redpajama_set_name: RedPajamaCommonCrawl


From design, through production and shipping, industry experts use these materials to successfully manage the manufacturing of their collections.
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Here's to new and exciting opportunities in 2016 - Happy New Year!
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How Can You Keep Up With Today's Complex Telecom Services?
Companies are demanding that service providers "get real" about the level of communications services necessary for today's complex environments and demands.
An article on Avaya's blog explains how technology developments are providing organizations with more powerful communications capabilities. The challenge is finding the resources, talent and training to keep up with these powerful tools.
Field services agencies are more likely to use subcontractors in situations that involve remote offices. They are also more likely to use technologies in which the prime partner isn't as proficient.
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Vernitta Easton decided to pursue a career in teaching after working a shift job that wasn't fulfilling.
Vernitta Easton completed the Bachelor of Education (Teaching) Primary specialisation, Bachelor of Education (Honours) and Master of Education before becoming a teacher.
"Before I studied teaching I was working a shift job that was not fulfilling. I wanted to study to get a decent career. I was also working as a teacher aide at the time and the teacher I was working with encouraged me to become a teacher. I chose to study with the University of Auckland as it has the best reputation and it offered me the chance to study closer to my home.
"It also fit into my schedule of being a mum and caring for a school aged child. It was very flexible. The Manukau campus has a unique atmosphere, it is friendly and more like a family than you would think, you are known as a person not just a student. I was able to make friends and have valuable one on one time with lecturers. The support we were given was overwhelming and the lecturers were very approachable.
"While I was studying I applied for and won a summer study scholarship which enabled me to help Professor Christine Rubie-Davies with her research. This allowed me to meet prominent lecturers in the gifted and talented field. The opportunity also motivated me to go for my Master of Education.
"My supervisors in my honours and masters were the best in their field and the support they gave me was appreciated beyond words. Without them I would never have finished. I am currently working at Willowbank School. This is my second year and I am teaching Year 3 students.


B2+ German Studies In ATS3098, I am listening to and improving my comprehension of radio and television programmes, talk shows, webcasts and video blogs. I can increasingly recognize colloquial expressions and differentiate between formal registers and idiomatic expressions. I am listening to songs and watching video clips. I am also accessing competency-level based selected online listening (audio and video) materials from a designated German as a Foreign language resource pool. Listening material ranges from 5-10 minutes in length. In ATS3098, I am developing a better understanding of different genres, including product information texts and long, complex instructions. I can recognize and discern between different points of view. I am developing more advanced German foreign language reading skills, including excerpting, skimming and scanning. In ATS3098, I am conducting interviews and talking on the telephone. I can describe internal states and generate formal and factual discourse as well as utterance reflecting internal states (emotions – distress, hope, anticipation disappointment, exasperation etc). I am able to sustain verbal interactions for up to 20 minutes and am preparing for 15 minute oral exams. In ATS3098, I am also developing more advanced expository and argumentation skills in context of oral presentation, as part of the final exam.My fluency is improving and my utterances are of a standard where I can discuss a text's content (article, blog, poem, prose) during 20 min session, as part of the final exam. In ATS3098, I am able to analyse discussions systematically. I am writing summaries, formal letters, stories, or advertisements. I am producing my own written narratives (up to 500 words) and have at my disposal a choice of registers and style. I am developing mastery textual coherence and cohesion.
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Which is how live music and wine at Arbor Crest turned into beer and fries at a college bar. No complaints here!
Why is it that on girls nights I always end up taking bathroom photos with these two? Ha!
Many laughs later the night ended!


text:
Infekční burzitida (IBD) je hospodářsky velmi závažné virové onemocnění kura domácího. Virus IBD primárně postihuje Fabriciovu burzu a poškozuje lymfatické buňky (B-lymfocyty) odpovědné za vznik humorální imunity. U mladých kuřat dochází k přímým ztrátám úhynem, rekonvalescentní nebo subklinicky infikovaná kuřata se opožďují v růstu, mají nižší hmotnostní přírůstky, sníženou odolnost vůči oportunním infekcím a nedostatečně reagují na vakcinaci proti jiným chorobám. Onemocnění je rozšířeno celosvětově.
První výskyt byl zjištěn v oblasti Gumboro ve státě Delaware v USA v roce 1957. Proto se onemocnění také často označuje jako "nemoc Gumboro". Podle zákona o veterinární péči č. 166/1999 Sb. je IBD považována za nebezpečnou nákazu.

Příčiny nemoci (etiologie)

Charakterizace viru
Původcem onemocnění je virus z rodu Avibirnavirus a čeledě Birnaviridae. Je to neobalený virus o průměrné velikosti 58-60 nm, s ikozaedrální symetrií kapsidy, tvořené 32 kapsomerami. Gradient hustoty virových partikulí v CsCl je 1,31-1,34 g/ml, přičemž nižší hustota odpovídá nekompletním partikulím. Genom viru infekční burzitidy (IBDV) se skládá ze dvou segmentů dvouvláknové RNA. Segmentace RNA je prokazatelná elektroforeticky v polyakrylamidovém gelu (SDS-PAGE). Oba segmenty migrují stejně, ale jsou rozdíly v migraci segmentů virů odlišných sérotypů, čehož se využívá při jejich diferenciaci. Virus obsahuje 4-7 proteinů; za hlavní se považují strukturální proteiny kapsidy označované jako VP2 a VP3. Antigenní determinanty (epitopy) pro sérotypovou specifitu jsou lokalizovány na proteinu VP2. Protein VP1 pravděpodobně představuje RNA polymerázu a VP4 virovou proteázu.

IBDV nemá hemaglutinační vlastnosti; pomnožuje se v cytoplasmě infikovaných buněk. Vyznačuje se vysokou odolností k podmínkám vnějšího prostředí, což umožňuje jeho dlouhodobé přežívání v chovném prostředí navzdory mechanické očistě i používání dezinfekčních prostředků. Virus je inaktivován jodovými preparáty, 0,5% roztokem chloraminu za 10 minut, 1% roztoky formalinu, kresolu a fenolu a 0,2% kyselinou peroctovou.

Laboratorní systémy
Virus IBD se pomnožuje v kuřatech, kuřecích embryích a buněčných kulturách, např. kuřecí embryonální buňky z Fabriciovy burzy, ledvin a fibroblasty.

Patogenita
V terénu se vyskytují dva sérotypy viru IBD. Jejich identifikace se provádí křížovými neutralizačními testy a křížovými čelenžními testy. Virusy sérotypu 1 jsou patogenní jen pro kura domácího, virusy sérotypu 2 se vyskytují u krůt i kura domácího a jsou apatogenní. Snyder et al. (1988) prokázali u virů IBD sérotypu 1 antigenní shift.

Patogenita izolátů IBDV se pohybuje od přirozeně apatogenních až po silně virulentní, které jsou schopny vyvolávat vysokou mortalitu kuřat s těžkým poškozením lymfatické tkáně. V posledních letech se začaly v terénu vyskytovat tzv. variantní kmeny IBDV, které se liší nejen virulencí i antigenně od izolátů klasického viru sérotypu l. Očkovací kmeny virů používané ve vakcinách se také liší virulencí. Vakcíny obsahující v různém stupni oslabené (atenuované) klasické kmeny viru typu 1 nechrání před infekcí variantami kmeny viru. Očkování sérotypem 2 nechrání před infekcí sérotypem 1.

Vznik a příčiny nemoci (epizootologie)

Přirozený a experimentální hostitel
Přirozené infekce virem IBD sérotypu 1 způsobují klinicky zjevné onemocnění IBD pouze u mladých kuřat (bez rozdílu plemen a užitkového typu). U dospívajících a dospělých slepic a kohoutů probíhá infekce subklinicky. Kur domácí, krůty, pštrosi a kachny pižmové jsou považováni za asymptomní nosiče viru IBD sérotypu 2. Protilátky proti IBDV byly prokázány také u perliček, bažantů, pštrosů, divokých kachen, husí, rybáků, papuchulků, vran a tučňáků. Předpokládá se, že divocí ptáci mohou sloužit jako rezervoár nebo vektor infekce. Z laboratorních zvířat jsou k infekci vnímavé pouze sající myši; králíci, morčata a křečci jsou refrakterní.

Přenos
Virus IBD je velmi nakažlivý a v hejnu vnímavé drůbeže se rychle šíří. V přirozených podmínkách se drůbež nakazí přes dutinu ústní (orálně), dutinu nosní (nazálně) či spojivku oční (konjunktiválně). Přenos se uskutečňuje horizontálně přímým kontaktem s nemocnou drůbeží nebo nepřímo stykem s kontaminovaným prostředím či předměty. Jako vektor se pravděpodobně uplatňují i členovci (obilní brouci, komáři) a potkani. Kontaminované prostředí (haly, krmivo, voda, trus) zůstává infekční nejméně 55-122 dní i po vyskladnění infikované drůbeže. Zatím neexistují důkazy o vertikálním přenosu infekce. Nelze ale vyloučit možnost kontaminace vaječné skořápky násadových vajec a tím i sekundární infekci vaječného obsahu, či zavlečení infekce do líhní kontaminovanými předměty nebo i personálem.

Patogeneze
Cílovými buňkami viru IBD jsou zejména nezralé prekurzorové buňky B-lymfocytů převážně ve Fabriciově burze (FB) a v menší míře také ve slezině, brzlíku a cekálních tonzilách, kde se virus pomnožuje a vyvolává zánětlivé změny. V důsledku masivní destrukce lymfatických uzlíků (folikulů) ve FB se snižuje množství zralých (imunokompetentních) B-lymfocytů a tím i produkce humorálních protilátek, takže dochází ke snížení imunity (imunosupresi). Buňkami zprostředkovaná imunita zůstává relativně nepoškozena.

Snížení obranyschopnosti je největší u kuřat infikovaných do 3. týdne věku; se stoupajícím věkem při infekci se negativní vliv viru IBD snižuje. Čím je virus virulentnější, tím je stupeň imunosuprese vyšší. Kuřata s pasivní imunitou získanou od imunních nosnic jsou chráněna v prvních 2-3 týdnech života. Někdy ani přítomnost pasivních protilátek nezabrání silně virulentnímu terénnímu viru v poškození FB a následnému snížení imunity.

U infikovaných i rekonvalescentních kuřat byla prokázána jak zvýšená vnímavost k infekcím, tak i snížená protilátková odpověď na vakcinaci proti mnoha různým chorobám. V infikovaných chovech bývá pozorován těžší průběh kokcidiózy a nižší účinnost antikokcidik.

Projevy nemoci (symptomatologie)
Onemocnění se může vyskytnout u kuřat masného i nosného typu v období mezi 3. až 15. týdnem věku; nejčastěji bývají postižena kuřata ve věku 3-6 týdnů, tj. v období po vymizení pasivních mateřských protilátek a v období vrcholící aktivity Fabriciovy burzy. U drůbeže imunní nebo starší je průběh subklinický.

Inkubační doba u vnímavých kuřat činí 2-3 dny.

Klinika
Jako typický průběh v neimunních hejnech kuřat se udává náhlý vznik nemoci a k úhynům dochází již za 1-2 dny po výskytu klinických příznaků. Mortalita prudce vrcholí a opět rychle klesá. Onemocnění trvá krátce (5-8 dnů). V současné době, kdy většina rozmnožovacích chovů (RCH) kura je vakcinována a kuřata mají pasivní protilátky, není průběh nemoci tak typický. Nemocnost kuřat je různá - podle stavu jejich imunity, věku při infekci a hygienických podmínek chovu. Může dosahovat i 80-100 %. Obdobně je ovlivňována mortalita - pohybuje se od 1-30 %. Nemoc se šíří mezi halami relativně pomalu. V zamořených objektech se často vrací i po kompletním vyskladnění hejna a dezinfekci hal. Rekurentní (návratné) infekce mají obvykle lehčí průběh.

Prvním příznakem u nemocných kuřat bývá bělavý, hlenovitý nebo vodnatý průjem, který zhoršuje kvalitu podestýlky. Peří je neupravené, načepýřené a znečištěné výkaly kolem kloaky. Kuřata si často sama nebo navzájem oklovávají kloaku, která může být v různém stupni zalepená výkaly. Shlukují se pod tepelnými zdroji, jsou netečná, ospalá, posedávají se svěšenou hlavou a zavřenými očními víčky, případně kulhají nebo leží na boku a nerada se pohybují. Nepřijímají vodu ani krmivo a hynou vyčerpáním. Krátce před úhynem lze zjistit třes těla a subnormální tělesnou teplotu. Potíže s vyprazdňováním kloaky i kulhání bývají způsobeny zánětlivě zvětšenou Fabriciovou burzou, která tlačí na ledviny, střevo i nervový plexus sacralis.

U kuřat mladších 2-3 týdnů probíhá někdy infekce v subklinické formě. Lymfatická tkáň je sice postižena, ale jediným pozorovatelným znakem infekce je silně zmenšená Fabriciova burza u uhynulých kuřat. Tato forma onemocnění je velmi závažná, protože způsobuje silnou imunosupresi a chybí signální klinické příznaky.

Patologie
Nález na FB je charakterizován akutním zánětem, jehož dynamika má typický (patognomický) průběh. Krátce po infekci se burza zvětšuje a její původní šedobílá barva se mění na krémově žlutou až žlutohnědou. Burza je překrvená, oteklá, s podélným žíháním na povrchu. Vzniká zánět a edém. Na sliznici a někdy i na seróze burzy se nalézají krváceniny různé velikosti i rozsahu. Dutina FB je vyplněna hlenovitým, případně krvavým a později až sýrovitým výpotkem (exsudátem). Za 3-4 dny po infekci zvětšení FB vrcholí a začíná se zmenšovat (atrofovat). Otok mizí, burza šedne a nabývá tuhé konzistence. Objevují se nekrotická ložiska a ojediněle přetrvávající krváceniny. Za 8-10 dní po infekci se velikost FB zmenší až na 1/3 své původní velikosti i hmotnosti. Atrofie burzy je obvykle trvalá. Dynamika změn je stejná i při subklinických infekcích. Silně virulentní kmeny IBDV způsobují pouze atrofii Fabriciovy burzy.

Změny na ostatních orgánech jsou druhotné a jejich výskyt není pravidelný - dehydratace, krváceniny různého rozsahu i stupně v podkoží, kosterní svalovině běháků, prsou a křídel, na serózách a sliznicích v místě přechodu svalnatého a žláznatého žaludku, perikardu a cekálních tonzilách. Ledviny mohou být zvětšené, s močovody naplněnými uráty. Slezina je někdy zvětšená, jindy naopak atrofovaná. Játra mohou být zvětšená, někdy i s ložiskovými nekrózami. Byl pozorován i zrnitý vzhled pankreatu a nedokrevnost kostní dřeně.

Mikroskopický nález ve FB odpovídá stupni postižení kuřat i pitevnímu nálezu.

Imunita
Virusy obou sérotypů vlastní skupinově specifický antigen, který je prokazatelný imunofluorescencí, imunodifuzí a ELISA testy. Sérotypově specifické antigeny indukují již za 3-4 dny po infekci tvorbu neutralizačních protilátek, které přetrvávají celoživotně. Precipitační protilátky vznikají stejně rychle, ale zanikají dříve. Imunní nosnice předávají přes vejce protilátky na potomstvo, které je tak chráněno před časnou infekcí a imunosupresí. Pasivní chráněnost kuřat však může být prolomena variantními anebo virulentnějšími kmeny viru. Poločas rozpadu mateřských protilátek je 3-5 dní.

Diagnostika
Diagnóza akutně probíhajícího onemocnění kuřat se stanovuje na základě náhlého vzniku nemoci v určité věkové skupině kuřat, vysoké morbidity a krátkodobé mortality charakterizované jednovrcholovou křivkou a rychlým uzdravením hejna během 5-7 dnů. Diagnózu lze podpořit pitevním vyšetřením Fabriciovy burzy, průkazem virového antigenu v burze, izolací viru nebo u rekonvalescentů sérologicky. Subklinické infekce se prokazují sérologicky nebo mikroskopickým vyšetřením Fabriciovy burzy.

Diferenciální diagnostika. Onemocnění infekční burzitidou se diagnostikuje na základě typických změn Fabriciovy burzy v průběhu akutního stadia. Atrofie burzy, ke které dochází po klinickém onemocnění nebo při subklinickém průběhu IBD, může být přítomna i u jiných onemocnění, jako jsou např. Markova nemoc, mykotoxikózy, infekční anémie kuřat nebo reovirové infekce. Mikroskopický nález v burze se však liší od změn při IBD.
Vzhledem k tomu že virus IBD snižuje imunitu hostitele, je infekce považována za predispoziční faktor pro jiná onemocnění, jako jsou gangrenózní dermatitida, inkluzní hepatitida kuřat a respirační nemoci. Spoluúčast IBDV na vzniku těchto nemocí je zpravidla diagnostikována až retrospektivně zjištěním změn na Fabriciově burze nebo sérologicky.

Terapie a prevence
Terapie IBD není známa. Vzhledem k vysoké odolnosti viru ve vnějším prostředí je prevence obtížná. Je nutné zabránit styku s infikovanou drůbeží i kontaminovaným prostředím. Nezbytností je striktní dodržování preventivních opatření k zabránění šíření nákaz. K dezinfekci jsou doporučovány 5% roztok formaldehydu, chloramin nebo jódové preparáty.
Základem kontroly výskytu nemoci je specifická imunoprofylaxe. Používají se živé oslabené vakcíny o různém stupni virulence vakcinačního kmene viru a inaktivované olejové vakcíny. Kuřata se vakcinují po vymizení mateřských protilátek a revakcinují se před dosažením pohlavní dospělosti. Univerzální vakcinační program nelze stanovit - měl by vycházet z konkrétních podmínek odchovu, zoohygieny, stavu aktivní imunity rodičovského hejna a hladiny mateřských protilátek u kuřat. Hlavním problémem při aktivní imunizaci kuřat s pasivními protilátkami je stanovení doby vakcinace. Ta závisí na výši mateřských protilátek, způsobu vakcinace a virulence vakcinačního viru. Nejvhodnějším řešením je monitorování humorální imunity v rodičovských chovech i jejich potomstva.

Literatura

Externí odkazy

Reference

Nemoci ptáků

redpajama_set_name: RedPajamaWikipedia


So in the last two weeks (of typing this), a kayaker was found drowned in Haulover Canal on the northern end of the Space Center and a British family was killed while attempting to maneuver through the crowds queued up to watch a SpaceX Falcon rocket on the way to the International Space Station.
Safety is a serious subject.
To be honest, I sort of cringe at the subject of safety because it is far too often over emphasized.
Below is a random selection of safety advice---Paddling on the water around the space center, and some advice while "in" the water or even getting to the water---or to view an inspiring rocket launch.
Well of all the safety factors, alligators get the most attention- because of the biggest teeth?
The threat is overrated- if you view attacks from a purely statistics.
Still it never hurts to be cautious.
American Alligator, a common sight on the Space Coast. They are not hunted in the protected waters of the space center and grow old and big.
Little too much warning, but some folks push the limits!
Please stay away and do not feed!
And not enough focus on a more common problem: Stingrays!
ALWAYS shuffle your feet (the sting ray shuffle) when walking or wading in the Indian & Banana Rivers, Mosquito Lagoon and their fresh water tributaries. The sting rays are there, they are scared of you and they will flee.
Just shuffle your feet and they will scoot-You will never even see them. But step on one and the barbed stinger in their tail will ruin your vacation.
In the photo a ray waits for mud minnows to ambush.
However, I have observed that one percent of sting rays that will sit in their sandy depression when you walk up to them and I had to nudge them away.
And so because of that one percent of rays that will not flee, you must always shuffle your feet in these waters.
A very painful wound results in a sting by the barbed tail of this shy fish.
Make the sting ray shuffle (drag or shuffle your feet) part of any time spent in salt or brackish waters.
Central Florida is one of the so called Lightning Capitals of the world.
Kennedy Space Center has rules that all outside work will cease when lightning is within 5 miles.
What if you are out on the lagoon or river?
This summer storm on the distant shore is a perfect example of (---add cuss words) This little cloud changed quickly into a big dark monster that caught us before we could get off of the water. Bringing scary lightning, rain and a serious change in wind directions.
Nowadays you can monitor your cell phone for the weather change, but always have an 'escape' plan to get off of the water in a lightning storm.
Pine Island on the Indian River is often my "get off the water" lightning escape location.
Insect repellent is kept in the boat just for those times when I have to get off the water & into 'real' Florida.
Use of the Personal Floatation Device when on the water.
Forget for the moment, that having one your boat is the law and it is never put on.
The Space Center "No Motor Zone" was set aside for Manatee habitat and has the largest concentration of manatees outside of winter springs or power plant hot water.
And so these cute, large herbivores will flip your boat in an instant if you scare them. Often they will be sleeping as you paddle over them.....and "splash"--- that fast you and your lunch are wet in astonishing big splashes of water.
It is not uncommon for a kayaker to be tossed from their boat in a manatee rodeo!
Beware startling the gentle giants of the Space Center!
The NASA Causeway page has the story of my wife being flipped from her kayak.
The point of this photo? Wear your PFD at all times when on the water!
In an instant, you paddle over a sleeping manatee and the world will turn white with spray, waves and foam as these endangered mammals use their huge tail flukes to escape from you in manatee panic.
PFD. PFD. Life Jacket. PFD. Use it, love it.
About the paddler who just died at Haulover Canal (the canal that connects Mosquito Lagoon and the Indian River. I do not know if he was wearing his PFD, however they did not find him right away. So I suspect he was not.
I also know that Barris Cove at the Haulover launch is saturated with happy-happy mating manatees and manatee families drinking at the fresh water seep under the manatee observation deck.
Another subject to consider. A paddle partner. The above mentioned paddler was last seen paddling by himself.
Full discloser: I am guilty of this nearly every time on the water. I am a solo paddler, night and day paddling 20 miles at a time when training for the MR340 kayak/canoe race.
I have attended many kayak meetups, events and races, but go solo most often because most people want to put in three miles and take a break. Then three more before heading to a brewery.
My PFD "Life Jacket" is on at all times on the water and I consider myself a strong swimmer. The point is the PFD is for when the unexpected happens.
However I do understand, acknowledge that a paddling partner is nearly as important as a PFD.
And speaking of PFDs! I just returned from a four day paddle on the Suwannee River.
At one of the exposed shoals, another kayaker was tipped over by the rocks. She was wearing an inflatable PFD in which she had removed the automatic water sensor that inflates the Life Jacket when immersed in water.
Consequently as she struggled in the rapids to retain her paddle, boat, composure, she was also attempting to manually inflate her PFD.
Life Jackets are for those unexpected emergencies. In some events you will not be conscious to "puff up your PFD" with salvaged breaths of air.
And I was going to opine on rocket viewing....Let me apologize for being long winded on the PFD subject.
Sometimes I can obtain a car placard to watch the upcoming launches.
In the extreme, unlikely event of an anomaly, the Emergency Operations Center will give directions over the KSC Paging and Area Warning System.
Sure this is a possible problem -I have witnessed two Atlas rockets blow up.
However the real danger is Traffic before, during and after the rocket launches.
People are lost, they may be tired or suffering from heat related problems.
They may be drunk-as I noticed on my MR340 practice paddle to watch the last night shuttle launch.
The flying swimming Giant Water Beetle.
I think they hit the VAB at night while flying. Because in the summer (wet) months they are piled up at the base of the VAB......Don't know why.
--Do know that as kid I tried to and succeeded in catching a Giant Water Beetle--predators extraordinaire.
Yes well I screamed for a long time.
Ok, so 99% of all snakes in Florida are completely harmless.
Reminder: You are in their home. The Space Center is a wildlife refuge and Eastern Diamondback Rattlesnakes are a protected species.
(Photo credit: NASA) Picture taken at the entrance to the Space Station Processing Building.
Well no..... This is the drag marks of a big alligator scooting into a canal.
Remember---you are a vistior in their home.
Return home from Indian River Lagoon Safety.


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~ William Makepeace ThackerayIf you are curious about this image, this is our garden table which has a dark tinted glass top and picked up the reflection of my house in it perfectly.
It's a wonderful photo. I love it!


I really love interior posts, but I find it so hard to get the real feel of the room across - so I hope I've done ok at portraying it! I've gone for a slightly more colourful version of my previous rooms, adding in some colour with neon lights, rugs and of course my millions of plants. I thought I'd share some snaps of my bedroom in my current flat as I like to do these posts sporadically and it's fab to look back at when I move.
I'd been after some art for my walls for ages, so when Desenio popped me an email I naturally jumped at the chance and went straight to their site to pick out some of my favourites. I ended up going for the four pictured, and I love them! I decided to frame three of them and hang one using a gold clip, which is an idea I pinched from their website. I quite like that it looks laid back amongst the other bits and bobs on my desk. There are definitely a few more on my wishlist so expect some gallery wall going's on very soon.
For now I've adhered them to the wall using Command strips which are so good as they don't require holes etc and come off clean - perfect for renting (not spon - just fab!) and they've worked with Desenio's frames perfectly.
So what do you think, wall art - yay or nay?


I work in the music industry, and successfully use Facebook, Twitter, Youtube, Soundcloud, Mailchimp, Wordpress and Pozible to run my career.
I own a Macbook Pro and an iPhone 5.
I am generally an organised, motivated and cool person.
Now, it's hard for me to say this—it feels like I'm bringing a six-pack of UDLs to a wine appreciation night.
Not only that, I have TWO Hotmail accounts, one with a nickname my friend gave me in 2003.
And, my dearest Gmail-Nazi, I don't care.
For me, it comes down to this. If someone sends me an email, I get it. If I send them an email, they get it. I use Mac mail, whose search history function is so munted it doesn't matter where your account is from anyway. SO IT DOESN'T MATTER.
Why would I waste hours of time "setting up a Gmail account", "importing contacts", "changing my email account for all logins", and "telling everybody about it". In reality, the outcome is the same: I send an email, the person gets it. If they send an email, I get it.
Except, of course, I would be WAY COOLER because I would use Gmail. Never mind the fact that it's the content of my emails and the accompanying pictures of dogs in hats that should establish my coolness.
Judging someone for their email host of choice is just plain snobby, in my opinion, and says more about YOU than me, really. Because you must love Google so much that they RUN YOUR LIFE and one day they will hand all your things over to the government and YOU WILL BE SORRY.
Meanwhile, I will be repeatedly unable to get my SMTP and POP servers to work. But at least I will be FREE.
The radical hippopotamus "Pepe", a particularly violent member of Pablo Escobar's Colombian drug cartel, was arrested after his Gmail correspondence was intercepted. Image credits: [Hippopotamus, Egypt] by T P Bennett, 1915. Lantern slide. State Library of Victoria, H83.103/122, available at http://handle.slv.vic.gov.au/10381/43413.
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Die Ligue de hockey junior majeur du Québec (kurz: LHJMQ, engl. Québec Major Junior Hockey League, kurz: QMJHL) ist eine der drei kanadischen Top-Juniorenligen, die die Canadian Hockey League bilden. Sie ist in der Provinz Québec beheimatet, beherbergt aber auch Teams aus den Seeprovinzen.

Organisation
Neben der Ligue de hockey junior majeur du Québec existieren in der Canadian Hockey League die OHL und die WHL. In Kurzform wird sie auch oft La/The Q genannt. Die Liga, die zur Saison 2013/14 insgesamt 18 Teams umfasst, wird in drei Divisionen mit jeweils sechs Mannschaften ausgespielt. Als neuestes Franchise wurde zur Saison 2012/13 der Phoenix de Sherbrooke in die LHJMQ aufgenommen.

In der LHJMQ dürfen nur Spieler zwischen 15 und 20 Jahren spielen. Jedes Team hat jedoch die Möglichkeit, drei Spieler einzusetzen, welche beim Saisonstart bereits 20 Jahre alt sind. Diese werden als "Overage"-Junioren bezeichnet.

Die LHJMQ brachte Stars wie Mario Lemieux, Patrick Roy, Martin Brodeur oder Sidney Crosby hervor.

Mannschaften und Herkunft der Teams

Meisterschaft
Der Meister der LHJMQ erhält die Coupe du Président als Trophäe. Die bisherigen Sieger waren:

Insgesamt gelang es zehn Teams der LHJMQ, den Memorial Cup, die offizielle kanadische Juniorenmeisterschaft, zu gewinnen: Den Remparts de Québec (1971, 2006), den Cornwall Royals (1972, 1980, 1981), den Prédateurs de Granby (1996), den Olympiques de Hull (1997), dem Océanic de Rimouski (2000), den Saint John Sea Dogs (2011, 2022), den Cataractes de Shawinigan (2012), den Halifax Mooseheads (2013), den Titan d'Acadie-Bathurst (2018) und den Huskies de Rouyn-Noranda (2019).

Auszeichnungen
Mannschaftstrophäen:
 Coupe du Président – Sieger der Playoffs
 Trophée Jean Rougeau – Bestes Team nach der regulären Saison
 Trophée Luc Robitaille – Meiste erzielte Tore
 Trophée Robert LeBel – Bester Gegentorschnitt

Individuelle Trophäen:
 Trophée Michel Brière – Wertvollster Spieler (1972/73)
 Trophée Jean Béliveau – Bester Scorer (1969/70)
 Trophée Guy Lafleur – Wertvollster Spieler der Playoffs (1977/78)

 Trophée Jacques Plante – Bester Gegentorschnitt (1969/70)
 Trophée Guy Carbonneau – Bester defensiver Angreifer (2004/05)
 Trophée Émile Bouchard – Verteidiger des Jahres (1975/76)
 Trophée Kevin Lowe – Bester defensiver Verteidiger (2004/05)
 Trophée Michael Bossy – Bester Professional Prospect (1980/81)
 Coupe RDS – Rookie des Jahres (1991/92)
 Trophée Michel Bergeron – Offensiver Rookie des Jahres (1969/70)
 Trophée Raymond Lagacé – Defensiver Rookie des Jahres (1980/81)
 Trophée Frank J. Selke – Fairster Spieler (1969/70)
 Trophée Marcel Robert – Hochschulspieler des Jahres (1980/81)
 Trophée Paul Dumont – Persönlichkeit des Jahres (1989/90)
 Plaque Wittnauer – Bestes soziales Engagement (1992/93)

Trophäen für Trainer und Funktionäre:
 Trophée Ron Lapointe – Trainer des Jahres (1992/93)
 Trophée Maurice Filion – General Manager des Jahres (2005/06)
 Trophée John Horman – Funktionär des Jahres (1989/90)
 Trophée Jean Sawyer – Marketingdirektor des Jahres (1990/91)
 Trophée Denis Arsenault – Pädagogischer Berater des Jahres (2010/11)

Eingeklammert steht die Spielzeit, in der zum ersten Mal die jeweilige Auszeichnung verliehen wurde.

Weblinks

 Offizielle Website

Nachwuchsliga
Eishockeyliga in Nordamerika


text:
Hinge focuses on common connections that you and a potential partner share on Facebook. Which is great if you trust the judgment of your friends and family. Of course, some of us are trying to meet new people, far removed from our everyday lives. (Hinge may have come to understand that, since you no longer need Facebook to sign up.) The app also asks questions to help you match with better connections, which can be a plus for serious relationship seekers.
It's not perfect. The quiz show format won't appeal to everyone, and the slow burn and winner-takes-all aspect mean it's going to be a lot harder to get a date than in other apps. Also, featured dater spots are currently only open to straight women, so there's not much here for lesbians or gay men yet, though there are plans to expand to male bachelors and LGBTQ+ episodes. You also need to email the company to apply to be a featured dater, which means it's not exactly a pick-up-and-go app if you're wanting to be the featured dater. However, if you're bored of regular dating apps, or if you're simply attracted to the fun elements and the prospect of finding love is a bonus, then give Quiz Date Live a go.
The app itself is well designed and pretty straightforward. You need to set up your profiles; there's one for dating and one for networking. Once your profile is active you can review closely curated dating prospects or access the Social Mode that shows you different hotspots where other members are hanging out. Since the initial application process is so rigorous and Raya has a strict code of conduct, you will find members tend to be more respectful of others. They're also more invested in the app overall, meaning they're more likely to meet other members in person.
You can only add photos of yourself from Facebook or Instagram, though, which is kind of limiting if you're not very active on either. Also, while the friends-of-friends concept has a lot of benefits, it's also restricting. It's possible to run out of matches after 10 minutes of browsing, which is a letdown if you're actually enjoying the app or are serious about finding a date.
They say a conversation opener is a a question or topic that creates a dialogue between two or more people. These 'starters' are some of the most elusive and hard to think of, and without them one is left with awkward silence. A lack of starters is common at the beginning of a relationship, when speaking to someone not entirely familiar to the speaker, or when conversing with any female.
Wild provides users with the anonymity that other dating apps don't. For starters, there's no social login required. The app also goes the extra mile to verify its users (meaning, you'll need to send a photo of you giving a thumbs up, which is then checked against the photos you've posted to verify it's really you), helping to take the awkwardness out of meeting up with a potential hookup that looks nothing like her picture. You can also filter by intention, so that you're not wasting time sorting through matches who are here for something serious. Once you've found a hookup for the night, you can set your profile to invisible so that other users aren't messaging you when you're, uh, in the middle of something.
Don't be put off by the name: Hinge is the newest kid on the dating game block, known as the relationship app. To set up a profile, you need more than just photos and a one-line bio. Instead, you must answer three questions (e.g. "My childhood crush...Andrew Garcia") and potential matches can 'like' your answers and start up a conversation. Hurrah to the end of swiping.
LesbianPersonals was created with gay girls in mind, particularly turned-on gay girls. Join the community of more than 30 million (it won't cost you!) and you're sure to find at least one woman who gets your engine running. Once you do, use LesbianPersonals' public forums, private chat rooms, and live videos to strike up a conversation. You could also share some sexy photos and vice versa to see if the physical chemistry is there.
Cupid offers a wide variety of online dating services allowing members to interact with each other and access the content available on the Group's websites. These websites are intended to appeal to dating users of diverse ages, cultures and social interest groups. The Group's most heavily visited websites include http://www.cupid.com, http://www.flirt.com, http://www.benaughty.com, http://www.girlsdateforfree.com and http://www.datetheuk.com. The Group also promotes the niche brands http://www.datingforparents.com, http://www.indiandating.com and http://www.maturedating.co.uk.
Sincerity must be a fundamental feature of your reports. Relationships with other people must be clear and polite. If, for some reason you do not like someone or that person did not satisfy your expectations please say so openly, but respectfully and politely. Try to always be yourself because this will facilitate the beginning of a future relationship with the person that suits your personality.
Sexy flirting hardly gets any better than it does on FriendFinder-X because you can show off your moves in a variety of ways — from messaging to live chat and webcams. There's also a private photo sharing feature, so you can pick and choose who gets to see you in all your glory. FriendFinder-X is compatible with iOS and Android devices, so pick up your smartphone or tablet, download it, and get to it!
Most dating apps are fairly LGBTQ inclusive. Still, it's nice to have an app to call your own. Her is tailored to lesbian, bisexual and queer women. It's a worthy notion -- but the app has some bugs and glitches that made it frustrating to use. Most of my queer female friends have told me they found the app "just OK," and that they usually end up back on Tinder or Bumble. Still I checked it regularly for some time and had a few pleasant conversations with actual human beings. And isn't that all we're really looking for in a dating app?
She even met her current long-term partner on Feeld. "I think it makes our relationship healthier that we started out fully aware of one another's kinks and interests," Veronica says. "We didn't have to hide those facets of ourselves, and that made it easier—at least for me—to feel good about just getting to know him and figure out that we had a genuine connection."
Today, app-first options — and mobile-friendly sites with their own custom apps — from AdultFriendFinder to Zoosk have changed the game thanks to massive databases of loyal, active date-seekers providing plenty of fish in the sea to choose from. That means if you're looking for like-minded people, you won't have to swim very hard or very far. Now, you just need to find the right dating site or app to sign up for.
As you'd expect from any online dating option with that kind of lofty goal, Elite Singles gives you more than just a Facebook photo to base your opinion on. The site collects information about users' professions and appearances, so if you sign up, you get a chance to be as careful and thoughtful with your love life as you are when you're on the job.
When LuvFree.com says they're 100% free, they really mean it. From communicating with matches to sending virtual kisses to creating a friends list to seeing who's viewed your profile, you can do practically anything on this app. Available for Android, LuvFree.com strives to help singles meet new people near them or all across the world — the choice is yours! Whether you're looking for friendship, dates, relationships, or even marriage, LuvFree.com is there to make your journey an easy and fun one.
Hacke also recommends testing the waters before proposing a hookup. "Try to get flirtatious and see how she responds," he says. "As long as your would-be hookup partner is matching your flirtatiousness beat for beat without appearing put off or weirded out, you're not doing anything wrong. You can gradually escalate the nature of your flirting until you've advanced to sexual innuendo. At that point, if she still seems interested, you've got a green light of sorts to attempt to initiate a hookup."
Best of all, since the launch of the dating site in 2003 till now with the dating app, registration is free. Users gain instant access to all the features including sending and receiving messages on the go with the mobile app, match with other singles and see who wants to meet you, as well as discover who viewed your profile and review your matches.
What it'll cost you:  A basic account is always free. But there are some paid extras you can enjoy if you want a more premium experience. If you pay for the A-List membership, you can cut out the ads, you get more search options like body type and attractiveness, you can see everyone who likes you, and you can see who reads your messages, among other useful things.
Bumble is one of those dating apps that tries to shake things up. It'll match you like normal. However, women get to initiate chats first. She'll have 24 hours to do so and then the man will have 24 hours to reciprocate. In homosexual matches, either one can go first. Many have touted this as a way to weed out creepy people. However, we couldn't verify that one way or the other and it makes things a little difficult for male users. The app does, in fact, show you possible matches and it gives you the opportunity to talk to new people. It has problems, but it's still a cut above a lot of others. We do like it for non-straight people, though, since they do get the classic dating experience without any bottlenecks.
And of course, you'll want to be using the right dating app to get the job done. Luckily, you're looking for a casual encounter at a time when new apps and features are constantly evolving to help you find exactly what it is you're looking for. Not sure which apps to start with? Here are some of the best dating apps for hooking up, as well as our advice on how to use them to your advantage to get lucky tonight.
Who's on Match.com? Your neighbors, coworkers and more. Match.com members form a diverse, global community of singles who share common goals - to meet other singles, find dates, form romantic relationships and meet life partners. Young and old alike, gay and straight, from everywhere around the world, singles come to Match.com to flirt, meet, date, have fun, fall in love and to form meaningful, loving relationships.
If you don't have the patience to weed out matches that are explicitly looking for a no strings attached hookup, a quick search on FriendFinder-X will probably make you pretty happy. You can search for potential matches using filters that range from proximity, sexual preferences and even cup size. Too lazy to search? The app has a list of compatible profiles sent to you for your consideration.
OKCupid has a reputation as being the most popular data driven dating site, and they have the stats to prove it. The app has, basically, all the functions of their website in mobile form, including building your profile, checking out and rating other profiles, answering quizzes and chatting up potential dates. A welcome feature is the ability to upload photos from your phone directly to your profile, which means that fantastic photo you took with the tiger from the zoo can be immediately set as your profile picture. Just don't end up on Fedoras of OKCupid, please.
Hinge may seem like it plays second-fiddle to the likes of Tinder, but it has a pretty elite user base (99 percent of its daters went to college, for example). Hinge's CEO compared his app to Facebook, versus Tinder's Myspace—sometimes for interface reasons (Hinge is aimed at the college-educated set) and sometimes for class reasons (much has been written on the ways dating app algorithms may favor white people).
You'll start getting profile views and messages within moments of signing up on Flirt.com, but unless you pay, you won't be able to reciprocate any real actions. The welcome screen of your account displays a variety of other profiles for you to browse instantly, while notifications in the top navigation bar keep you posted on activities relating to you.
"The fun way to find people like you," LOVOO is one of the most popular apps for flirting, with more than 50 million members. You can register, upload and send photos, and chat with like-minded singles all without having to spend a single penny. Perhaps the coolest feature on LOVOO is their live radar that shows what's happening in your area and who's participating.
Like Raya, joining The League can take a bit of effort. You need to set up a profile and allow the app to access your Facebook and LinkedIn accounts. The League uses these networks to verify your information and to make sure colleagues do not see your account. After you complete your application, The League will verify your eligibility, and you will either be accepted on the spot (rare), rejected (common), or waitlisted. If waitlisted, it can take several hours to several months to become a full-fledged member.
The app does an incredibly good job at collecting feedback from singles, using it to help increase your rate of success. Members are given a report card-style dashboard that shows them why users liked or passed on their profile, down to the specific reason, such as low-quality profile pictures, as well as offering ways to improve their likelihood of getting "liked." For any online dater, this dashboard alone is a good (and free) tool to gauge how their profile is performing compared to others.
SpeedDateMate is where you'll find the best prices for upcoming speed dating and social nights — all at the press of one button. You can book tickets and filter events to suit your taste, wants, and needs, all with location awareness. During this series of mini face-to-face dates, which are held at venues across the country, you'll get to meet lots of potential matches, and you can even invite friends to make a night out of it!
Online dating has come a long way in a relatively short period of time. In the past, the online dating options available to singles (or people in open relationships) were fewer and further between. Worse, at the time, free online dating options were often either highly sketchy, putting your identity and privacy at risk, or simply did not have the membership numbers to give you a worthwhile experience.
That brings us to the end of the article on the 7 Best & Free Flirting Apps for Android & iPhone 2019. Just be a little aware of the details you share with the other singles on such platforms. At the same time, you should be under your limits and have respect while talking. Don't go overboard! Your one small mistake can cause a big problem later on. You never know what the future holds for you! So please take care of yourself by being a little smart and attentive while using and chatting with new people.
Why it's awesome: Plenty of Fish, sometime styled as POF, boasts 4 million daily active users, with 65,000 new users each day, apparently, and claims users send 1 billion messages per month. After registering for POF, hopeful daters take a personality test that then helps POF determine what they call, "Your Relationship Needs." Basically, it's a way to make sure users know what they want from their love lives, and to ensure that it serves users other profiles that meet that criteria. One unusual quirk: The site recently launched a feature that allows users to message others through Google Home. Says Spira: "They have a large user base, are a free site, and are very popular."
Within the first three hours of signing up, Happn welcomed me with 68 users it said I had crossed paths with, even though I hadn't left my apartment all day. It might be helpful if you're looking to date your immediate neighbors (or Uber drivers), but I struggle to see why this is much of a draw when competitors like Tinder already show the distance between you and other users. Frankly, if I saw a cute guy in a coffee shop, I'd rather just approach him than check if he's on Happn. The app seems designed for people who don't want to use online dating but who also don't want to approach people in real life. Pick a lane.
Another bonus is that AdultFriendFinder is like the dating site version of New York City (AKA it never sleeps). You'll find people who work the regular 9-5, people who work the night shift, and people in other time zones, so it's nearly impossible to log on and not have people to talk to. AdultFriendFinder is like the booty call that's always awake when you text them.
As a very distinguished flirt I have always been taught to consider her, but it has lately fallen In my way to hear some particulars of her conduct at Langford: which prove that she does not confine herself to that sort of honest flirtation which satisfies most people, but aspires to the more delicious gratification of making a whole family miserable.
The social chat service, Knuddels, saw about 808,000 email addresses and over 1.8 million usernames and passwords exposed after an attack in July; the perpetrators went on to publish the information online at Pastebin and the Mega cloud storage service in cleartext form. An investigation by regulators showed that the website stored its data in plain text with no safeguards – which Knuddels confirmed.
One of the easiest and most budget-friendly ways to dive into online dating is through the well-known Plenty of Fish, which functions as both a site and an app. Regardless of which device you're using it on, the platform provides a feature-packed online dating experience that doesn't cost you a dime. There's an abundance of members from all different walks of life, most of whom are continually active on the site. Plenty of Fish is designed for finding people for long-term relationships as well as arranging casual, no-strings-attached meetings, although it skews more toward the latter option.
Feeld is an app for people who know what they want. It describes itself as a place to "meet open-minded couples and singles near you," making it the premiere app for unicorns and those who want a more openly kink-friendly app experience. While that may sound pretty niche, Veronica*, 35, who lives in Queens, says Feeld became her favorite dating app. At the time of download, she was single and recovering from a bad breakup. She used Tinder and Bumble for regular dating, but hated the experience. "None of the men seemed cute enough, and a lot of them were exactly as gross and Air-Drop-a-dick-pic-slimy as the stereotypes go," she explains.


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News: AMRC and the factories of the future
By Tom Austen
Engineers at the University of Sheffield Advanced Manufacturing Research Centre (AMRC) are helping SMEs create factories of the future where humans and robots work together to boost productivity.
Collaborative robots – also known as cobots - differ from conventional industrial robots in that they feature technology that allows them to operate safely alongside human workers in a shared area. They are capable of operating with limited force and speed and are equipped with force-sensing to enable them to stop when they come into contact with an operator.
Traditionally, the safe use of robots in manufacturing has relied on barriers, cages and fences that keep humans and robots separated. The downside to this is that it uses lots of floor space and limits the work robots and humans can do together.
The Integrated Manufacturing Group at the AMRC's Factory 2050 on the Sheffield Business Park has been developing technology demonstrators and testing a new safety standard awaiting ratification for different types of cobots.
Supported by the Government's Catapult programme, the project began in May last year and completed in March this year. A cell was created at Factory 2050 which demonstrated to SMEs how they can integrate the technology onto their factory floors and transform production lines by increasing the rate at which a process is done, as well as the uniformity and quality of finish on a product.
Phil Kitchen, lead for the Collaborative Robotics research project, said: "What we want to do is to start to develop a gold standard for integration. To get this gold standard, for us, it would mean we could help SMEs to integrate cobots on to their shop floor.
"There are a number of benefits of collaborative robotics for SMEs – improved quality, improved rate, so the rate at which they are making their products - and if they improve rate they can potentially sell more and expand, helping them to grow through the use of collaborative robotics.
"A technical specification was released which detailed how you would go about integrating a collaborative robotic system. It's not yet been ratified as a full ISO standard to use and that is being worked towards now. Essentially what we are aiming to do is put ourselves in the right position to act when the technical specification is ratified by the HSE."
Phil said cobots come into their own when a process will always require a degree of human intervention – such as tasks requiring dexterous, manual manipulation or human decision making. Cobots are also typically lighter and more flexible than bulkier traditional robots, and are able to be moved around the factory floor.
He said: "Cobots can be used as a flexible resource and they differ from standard industrial robots as they have some element of safety built in. So for example, with the KUKA iiwa - that has force and torque sensors in each of its joints so if it touches you it can sense an external force being imparted on to it and will stop itself.
"Whereas with a regular robot, if you get in with it, it could cause serious injury. That's the main difference really, a collaborative robot has some sort of inbuilt technology to allow for safe human-robot interactions."
One demonstration involved a Tier 1 member of The AMRC process whose production line involved fastening components and apply adhesive to them. Using robots removed the need for operators having to use manual adhesive guns.
AMRC website
Images: AMRC
Labels: Advanced Manufacturing Park, Advanced Manufacturing Research Centre, business support, health and safety, Manufacturing, University
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Q: How to get more diagnostic output on a "no module named xyz" error? I have, in a local directory, a django project that works
fine when I run it from a local django server, as in
python manage.py runserver.py 8000

But if I clone the local git repository where this project
lives (and which is up-to-date), like this:
git clone my_django_project my_django_project_clone

and I try the same command above from the clone's directory, the
server fails immediately:
Error: No module named xyz

The server produces no other output at all, even when I run it
with the -v 3 flag.
Is there any way to get more diagnostic information on why
this (supposedly) identical clone of a working django site is
failing?

A: This is how I would approach the problem

*

*Start interactive Python prompt
 python -vvv   # -vvv notes to print out import trace information

*Type
 import xyz

If this works it means the module is valid Python installation. If it doesn't work with Django then it is a probably an issue with circular imports and problem with Django initialization code itself. Django does a lot of behind the scenes magic when it reads settings.py file, scans all Django applications and so on, so circular import is often the case why Python cannot load the module. Also this could be a case that Django swallows the original ImportError exception and does not print the nested exception what actually when wrong when initializating Django. In this case, manually typing import xyz should show you the real error.

*If the module loading fails then the next thing is to type
import sys
for p in sys.path: print(p)  # print p for Python 2.x

This will show all directories where Python pulls in modules. Your module isn't probably in any of these directories so you need to make sure
A) You append your own modules to Python sys.path list or use PYTHONPATH environment variable correctly to load your own packages. Settings up path may depend on Django version, Python version and how Django is run. For example the current working directory where you run manage.py command may or may not be included. If the module is in the current folder you can try this:
   PYTHONPATH=. python manage.py runserver

B) You install your third party packages properly. The proper way to install third party Python packages is using pip tool with virtualenv isolated Python environment for your development project. If you install Python packages to system-wide Python installation (/usr/lib/python on UNIX), especially using sudo pip, the result is not guaranteed to work.

A: You probably need to install the dependencies. A common thing is to store dependencies inside requirements.txt and run "pip install -r requirements.txt" when you clone a project. To push dependencies into requirements.txt you use "pip freeze > requirements.txt". So, if you project uses PIL or six or whatever it will be added to that file and then you can pip install them with a single command. I hope I haven't missed your point.

redpajama_set_name: RedPajamaStackExchange


text:
It's Breast Cancer Awareness Month — do you know where your pink ribbon is?
Published: Oct 25, 2007
Did you know that breast cancer is the most prevalent cancer in the world today? And it is the most common cause of cancer-related deaths among women. The good news is that, due to early detection and more effective treatments, mortality rates are declining.
In support of Breast Cancer Awareness Month, House Beautiful is featuring a pink ribbon — heralded as the worldwide symbol of breast health — at the top of each page. Our sister sites, Cosmopolitan, Country Living, Good Housekeeping, Harper's Bazaar, Marie Claire, Quick and Simple, Redbook, and Seventeen, are supporting the cause as well.
If just one woman, anywhere in the world, sees the pink ribbon on the House Beautiful Website and is inspired to get a mammogram or seek information about breast cancer, then our objective has been accomplished.
Click on the pink ribbon to visit the Breast Cancer Research Foundation's Website (bcrfcure.org, where you can learn more about the foundation, see its research in action, support its partners and programs, or participate in its events. The Website also offers breast cancer resources including an extensive Breast Health Library.
Read more about breast cancer in these related articles from our sister sites:
Cosmopolitan.com
Fashion for a Cure
GoodHousekeeping.com
Breast Cancer: New Ways to Beat the Odds
Think Pink: Shop for a Cure
Good News About Breast Cancer
MarieClaire.com
Best Beauty: Beat Breast Cancer
Burden of Truth
Liv Tyler and Emergen-C for Breast Cancer
QuickandSimple.com
Shop… Just Be-Cause!
Redbookmag.com
Laura Bush's Breast Cancer Crusade: Crossing Cultures to Find a Cure
Who Gets Breast Cancer and Who Survives?
Share Your Story: "How Breast Cancer Changed My Life"
Seventeen.com
Breast Cancer Awareness E-Cards
Sonia Kashuk Makeover Video
Shop for the Cure


text: Q: "DeprecationWarning: Buffer() is deprecated due to security and usability issues" when i try to install node.js when trying to install node.js 15.5.1 i get this error (node:21252) [DEP0005] DeprecationWarning: Buffer() is deprecated due to security and usability issues. Please use the Buffer.alloc(), Buffer.allocUnsafe(), or Buffer.from() methods instead. how can I correct for this? it opens a powershell and then after a bit it will give the error (I also cant ^C the thing it tells me to fix the code)


In a statement about the honor, GLAAD President and CEO Sarah Kate Ellis describes the power couple as 'passionate defenders of human rights and acceptance for all people'.
AceShowbiz - Beyonce Knowles and Jay-Z will be honoured with the Vanguard Award at the 30th Annual GLAAD Media Awards later this month.
The pop power couple will receive the prize for their "fair, accurate, and inclusive representations of LGBTQ people and issues" in their work and public statements, at a ceremony in Los Angeles on March 28.
"Beyonce and Jay-Z are global icons and passionate defenders of human rights and acceptance for all people," GLAAD President and CEO Sarah Kate Ellis said in a statement on their website. "When Beyonce and Jay-Z speak, the world becomes inspired and when it comes to LGBTQ people, their voices of acceptance have been heard loud and clear."
The issue is a personal one to the couple, as Jay's mum Gloria identifies as a lesbian and addressed the GLAAD Media Awards last year. She appeared on his 2017 track "Smile", in which he raps about his mother revealing her sexuality.
"'Smile' became a reality because I shared with my son who I am," Gloria told the audience at last year's awards bash. "My son cried and said: 'It must have been horrible to live that way for so long.' My life wasn't horrible. I chose to protect my family from ignorance. I was happy but I was not free."
Previous stars GLAAD, formerly the Gay & Lesbian Alliance Against Defamation, has honoured with the Vanguard prize include Jennifer Lopez, Kerry Washington, Cher, Janet Jackson, Elizabeth Taylor, Antonio Banderas, Demi Lovato, and Britney Spears.
"Will & Grace" actor Sean Hayes will receive the Stephen F. Kolzak Award at this year's ceremony, while among the nominees in 27 categories are Janelle Monae, Hayley Kiyoko, Troye Sivan, RuPaul, Stephen Colbert, and Samantha Bee.