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Animal Health Research Reviews
The efficacy of litter management strategies to prevent morbidity and mortality in broiler chickens: a systematic review and network meta-analysis
Part of: Antimicrobial stewardship in livestock
Published online by Cambridge University Press: 21 February 2020
Jan M. Sargeant [Opens in a new window] ,
Michele D. Bergevin ,
Katheryn Churchill ,
Kaitlyn Dawkins ,
Bhumika Deb ,
Jennifer Dunn ,
Dapeng Hu ,
Catherine M. Logue ,
Shannon Meadows and
Carly Moody
...Show all authors
Jan M. Sargeant*
Department of Population Medicine, Ontario Veterinary College, University of Guelph, ON, Canada Centre for Public Health and Zoonoses, University of Guelph, ON, Canada
Michele D. Bergevin
Katheryn Churchill
Kaitlyn Dawkins
Bhumika Deb
Jennifer Dunn
Dapeng Hu
Department of Statistics, Iowa State University, Ames, USA
Catherine M. Logue
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, USA
Anastasia Novy
Guelph Poultry Veterinary Services, ON, Canada
Annette M. O'Connor
Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, USA
Mark Reist
Yuko Sato
Department of Statistics, Iowa State University, Ames, USA Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, USA
Charlotte B. Winder
Author for correspondence: Jan M. Sargeant, Department of Population Medicine, Ontario Veterinary College, University of Guelph, ON, Canada. E-mail: sargeanj@uoguelph.ca
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A systematic review and network meta-analysis (NMA) were conducted to address the question, 'What is the efficacy of litter management strategies to reduce morbidity, mortality, condemnation at slaughter, or total antibiotic use in broilers?' Eligible studies were clinical trials published in English evaluating the efficacy of litter management in broilers on morbidity, condemnations at slaughter, mortality, or total antibiotic use. Multiple databases and two conference proceedings were searched for relevant literature. After relevance screening and data extraction, there were 50 trials evaluating litter type, 22 trials evaluating litter additives, 10 trials comparing fresh to re-used litter, and six trials evaluating floor type. NMAs were conducted for mortality (61 trials) and for the presence or absence of footpad lesions (15 trials). There were no differences in mortality among the litter types, floor types, or additives. For footpad lesions, peat moss appeared beneficial compared to straw, based on a small number of comparisons. In a pairwise meta-analysis, there was no association between fresh versus used litter on the risk of mortality, although there was considerable heterogeneity among studies (I2 = 66%). There was poor reporting of key design features in many studies, and analyses rarely accounted for non-independence of observations within flocks.
Broiler poultrylitter managementmorbiditymortalitynetwork meta-analysissystematic review
Animal Health Research Reviews , Volume 20 , Issue 2 , December 2019 , pp. 247 - 262
DOI: https://doi.org/10.1017/S1466252319000227[Opens in a new window]
This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © The Author(s), 2020
Litter management strategies serve a vital role in the poultry industry, with the potential to impact bird health, welfare, and productivity (Dunlop et al., Reference Dunlop, Moss, Groves, Wilkinson, Stuetz and Selle2016). 'Litter' refers to the combination of bedding material and fecal matter that typically covers the floor of commercial broiler operations (Shepherd et al., Reference Shepherd, Fairchild and Ritz2017). Two key functions of litter in the context of disease control are to absorb moisture from bird excrement, and to minimize exposure of birds to manure to prevent infections that may result in the need to use antibiotics (Shepherd and Fairchild, Reference Shepherd and Fairchild2010; Kaukonen et al., Reference Kaukonen, Norring and Valros2016). Poor litter management can result in the spread and persistence of diseases of major economic importance within and across flocks, such as coccidiosis, necrotic enteritis, and colibacillosis (Yegani and Korver, Reference Yegani and Korver2008). Reduced flock health due to wet litter (above 25–35% moisture) may result in decreased weight gain and lower feed conversion efficiency (de Jong et al., Reference de Jong, Gunnink and van Harn2014) and, in worst cases, increased mortality due to compromised respiratory or systemic health.
Prophylactic or metaphylactic antimicrobials may be used alongside litter management strategies to improve flock health by reducing the rates of infectious diseases (Chen and Jiang, Reference Chen and Jiang2014). However, it is imperative that antimicrobial use in human healthcare and in animal agriculture be limited to judicious use to reduce the potential for the development of antimicrobial resistance. In an effort to slow the development of antimicrobial resistance, the World Health Organization (WHO) recommends prudent use of antimicrobials in food-producing animals and in human medicine (WHO, 2015). Improved litter management is one strategy that could reduce the need for antimicrobials to control infectious diseases in the poultry industry. Understanding how litter management impacts morbidity, mortality, and antibiotic use in broiler chicken production will help producers to make informed management decisions that could reduce the need for antibiotics, while also maximizing the health, welfare, and productivity of the animals.
Systematic reviews provide a transparent and replicable method for identifying and summarizing research evidence from multiple studies (European Safe Food Authority, 2010; Higgins and Green, Reference Higgins and Green2011; O'Connor and Sargeant, Reference O'Connor and Sargeant2014; Sargeant and O'Connor, Reference Sargeant and O'Connor2014). When results are available from multiple studies that compare interventions against the same outcome, those results may be combined statistically in a pairwise meta-analysis (Higgins and Green, Reference Higgins and Green2011; O'Connor et al., Reference O'Connor, Sargeant and Wang2014). A pairwise meta-analysis compares the relative efficacy of two interventions, such as two types of treatments or a treatment and a control (Higgins and Green, Reference Higgins and Green2011). Although meta-analysis is useful for synthesizing pairwise comparisons, decision-making may be better informed by an evaluation of the comparative (relative) efficacy of all of the available treatment or intervention options. Network meta-analysis (NMA), an extension of pairwise meta-analysis, provides such a method for evaluating the comparative efficacy of multiple treatment options (Salanti, Reference Salanti2012). In addition, NMA uses information from all comparisons within an intervention network to produce indirect estimates of the relationships between interventions, thus increasing the amount of information available for efficacy evaluations. This analysis approach can also provide estimates of relative efficacy between interventions in cases where no direct comparisons between those interventions have been published. For instance, suppose that there are studies in the literature comparing the efficacy of wood shavings for litter to newspaper and comparing shavings to peat moss. Even if there are no studies directly comparing newspaper and peat moss, this relationship can be inferred indirectly based on the available information on the comparisons between shavings and newspaper, and shavings and peat moss, given certain assumptions (White et al., Reference White, Barrett, Jackson and Higgins2012). Thus, even in the absence of a complete set of direct comparisons between interventions in the literature, NMA can be a valuable tool to inform management decisions.
We used a systematic review and NMA to address the review question: 'What is the efficacy of litter management strategies in reducing morbidity, mortality, condemnation at slaughter, or total antibiotic use in broiler chickens?'. While performance measures, including feed conversion and flock uniformity, also are of key importance to growers and veterinarians, we focused our outcomes on those related to disease and therefore of direct relevance to antibiotic stewardship.
Protocol and registration
A protocol was prepared a priori and was reported in accordance with PRISMA-P guidelines (Moher et al., Reference Moher, Shamseer, Clarke, Ghersi, Liberati, Petticrew, Shekelle and Stewart2015). The protocol was published to the University of Guelph's institutional repository (https://atrium.lib.uoguelph.ca/xmlui/handle/10214/10046) and also is available through the Systematic Reviews for Animals and Food (SYREAF) website (http://www.syreaf.org/contact/). This review was reported using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for NMA (PRISMA-NMA) guidelines (Hutton et al., Reference Hutton, Salanti, Caldwell, Chaimani, Schmid, Cameron, Ioannidis, Straus, Thorlund, Jansen, Mulrow, Catalá-López, Gøtzsche, Dickersin, Boutron, Altman and Moher2015).
Primary research studies, both published and non-published (grey literature), available in English were eligible. In addition, eligible studies must have included the following elements based on the PICOS components:
Population (P): Broiler chickens in commercial or research flocks;
Intervention (I): Litter management strategies (as defined by the authors);
Comparator (C): Alternate litter management strategy, or a no intervention control;
Outcomes (O): Clinical morbidity (as defined by the authors), condemnations at slaughter, mortality, total antibiotic use;
Study design (S): Controlled trials with natural disease exposure and analytical observational studies.
The electronic databases searched were AGRICOLA (via ProQuest, 1970 to search date), CAB Abstracts and Global Health (via the University of Guelph CAB interface, 1900 to search date), MEDLINE (via PubMed; 1946 to search date), Conference Proceedings Citation Index – Science (via Web of Science, 1990 to search date), and Science Citation Index (via Web of Science, 1900 to search date). No restrictions were implemented in the search strategy related to publication date, language of publication, or study type. In addition, a single reviewer hand-searched all available table of contents of the Western Poultry Disease Conference and Poultry Science Association conference proceedings from 1980 to 2018.
The search strategy initially was developed for the Science Citation Index (Web of Science) and comprised search terms related to three concepts: broilers, litter management, and disease prevention. A list of relevant search terms was compiled for each concept within the search strategy; search terms within each concept were linked using the Boolean operator 'OR', and the concepts were linked using the Boolean operator 'AND'. The full search strategy as applied in the Science Citation Index website is provided in Table 1. Database searches were conducted through the University of Guelph library on 18 June 2018. Search results were uploaded to EndNoteX7 (Clarivate Analytics, Philadelphia, PA, USA) and duplicate records were identified and removed. Records were then uploaded to DistillerSR (Evidence Partners Inc., Ottawa, ON, USA) and again de-duplicated. DistillerSR was used for eligibility screening, data extraction, and risk of bias assessment.
Table 1. Full electronic search strategy used to identify studies of litter management strategies to prevent morbidity and mortality in broilers in Science Citation Index (Web of Science) conducted on 18 June 2018
Titles and abstracts were screened for eligibility. Reviewers were trained on a pre-test of the first 250 citations to ensure the clarity of understanding and consistency of application of the screening questions. Thereafter, two reviewers independently evaluated each citation, using the following questions to assess eligibility:
(1) Is this a primary study that evaluated litter management to reduce clinical morbidity, condemnations at slaughter, mortality, or antibiotic use in broilers?
Yes, No, Unclear
(2) Is there a concurrent comparison group? (i.e. controlled trial with natural or deliberate disease exposure or analytical observational study)?
(3) Is the full text available in English?
Citations were excluded if both reviewers responded 'no' to any of the questions; agreement was at the include or exclude decision level. Disagreements were resolved by consensus. Two independent reviewers conducted the full-text eligibility screening of remaining studies, using the first 10 citations as a pre-test. The full-text screening stage included the initial three questions with only yes or no (exclude) options, and additionally:
(4) Is the full text available with >500 words?
(5) What best describes the intervention?
Feed/nutritional associations with litter quality
Direct litter management (litter or flooring type, litter depth, use of fresh versus re-used litter) (NB: the inclusion of this question to broadly categorize the interventions represents a protocol deviation, which was included due to the volume of literature identified after title and abstract screening).
(6) Are at least one of the following outcomes described: morbidity, condemnations at slaughter, mortality, or antibiotic use?
(7) Eligible study design: what is the study design?
Analytical observational study,
Controlled trial with natural disease exposure,
Controlled trial with deliberate disease induction
Agreement for the full-text eligibility screening was at the question level, with conflicts resolved by consensus or by mediation by JMS or CBW if an agreement could not be reached.
Studies were included in the meta-analysis if sufficient data were reported to enable the calculation of the log odds ratio (OR) and the standard error of the log OR, based on the extraction of the prioritized metrics. Additional criteria for inclusion in the meta-analysis are described in the statistical analysis section.
Data collection process
There were a few protocol deviations at the data collection stage. First, as only three observational studies were identified, and controlled trials are considered a higher level of evidence, data were only collected from controlled trials with natural disease exposure. Second, due to the volume of literature and heterogeneity between the intervention types, we extracted data only from studies evaluating litter management (litter or floor type, litter depth, or used versus fresh litter). Studies which evaluated the indirect effects of feed or nutrition on litter quality were excluded at full-text screening as 'not the intervention of interest'.
Data from citations that were eligible following full-text screening were independently extracted by two reviewers using a standardized form, which was piloted on the first five articles by all reviewers. Discrepancies in data extraction were resolved by consensus, or by mediation by JMS and CBW if an agreement could not be reached.
Data items
Study characteristics
Study-level data that were extracted from each eligible trial included study design, publication year, country, month(s) and year(s) during which data were collected, whether the trial was conducted in a research or commercial flock, strain and sex of birds, number of flocks or farms enrolled, inclusion and exclusion criteria at the flock-level, and rearing conditions (e.g. conventional, organic, antibiotic-free).
Interventions and comparators
Details on the interventions, including a description of the intervention, number of birds/rooms/flocks enrolled, age when intervention was initiated, duration of intervention, and concurrent therapy were recorded. Losses to follow-up for each intervention group were captured when reported in the trial.
Outcomes and results
Data were extracted for clinical morbidity (as defined by the study authors), condemnations at slaughter, mortality, and total antibiotic use. It was anticipated a priori that there would be variation in the method of presentation of results, and that some trials would present the results in multiple ways. Therefore, the type of results to be extracted was prioritized such that only one set of results for each outcome were extracted per trial. The first priority for the extraction of results was an adjusted summary effect (adjusted OR or risk ratio (RR)). Adjustment referred to covariates or adjustment for non-independence in grouped housing. Variables included in the adjustment, and the corresponding precision estimates were recorded. If an adjusted measure was not reported, unadjusted summary effect size (second priority) or raw data (third priority) were recorded, with applicable variance components. Data presented without variance measures, and for which a measure of variance could not be calculated, were not extracted. If eligible outcomes were measured at multiple time points, data from the final time point were extracted.
Geometry of the network
We used a visual approach to qualitatively evaluate the geometry of the network, to determine if some pairwise comparisons were more common than others and to determine whether the network appeared to have a star or web-like structure. We also evaluated whether there were intervention comparisons that were not linked to the network (i.e. did not have an intervention in common with one or more other published trials).
Risk of bias in individual studies
The Cochrane risk-of-bias tool for randomized trials (RoB 2.0, 2016 version) was used to assess bias at the outcome level for all outcomes and all trials included in the NMA (Higgins et al., Reference Higgins, Sterne, Savović, Page, Hróbjartsson, Boutron, Reeves and Eldridge2016). This tool provides a framework for assessing the likelihood that bias could be introduced in each of the five domains. The domains of bias are: bias arising from the randomization process, bias due to deviation from intended interventions, bias due to missing outcome data, bias in measurement of the outcome, and bias in the selection of the reported result. Signaling questions are a component of the RoB 2.0 tool that is used to elicit information on the use of trial features that are relevant to the potential for bias in a trial. Signaling questions were modified for use in poultry trials. A question in the bias due to randomization domain in the Cochrane tool pertains to the method for generating the random allocation sequence. We modified this question to include a response category for studies where the authors reported that allocation to intervention groups was 'random', but did not provide details on the actual method for generating the random sequence. The RoB 2.0 domain bias related to deviations from the intended intervention has a question on whether the participants were aware of their treatment assignments; this always was answered as 'no', in that the 'participants' in these trials were broiler chickens. Another question in this domain asks whether study personnel were blinded, and for the purposes of this review, this question was clarified to refer to blinding of the animal caregivers.
The overall risk of bias within each domain was calculated based on the algorithms suggested by Higgins et al. (Reference Higgins, Sterne, Savović, Page, Hróbjartsson, Boutron, Reeves and Eldridge2016), with one exception. For bias due to the randomization process, we excluded a consideration of allocation concealment because all animals within a flock would be expected to be included in the type of trial involved in this review. Furthermore, it is unlikely that a producer or investigator would have any treatment preference for a given flock, as the differential economic value of a flock would not be known at the time of allocation. This approach has been used in a previous evaluation of livestock trials (Moura et al., Reference Moura, Totton, Sargeant, O'Sullivan, Linhares and O'Connor2019).
Summary measures
The baseline risk used to convert the ORs to the RR was obtained by using the distribution of the placebo group and a Bayesian approach method of estimation. For the model related to footpad lesions, posterior mean and standard deviation of the baseline risk mean were −0.3759 and 3.1808. The posterior mean and standard deviation of the baseline risk standard deviation were 2.9462 and 0.7094. For the model related to mortality, posterior mean and standard deviation of the baseline risk mean were −3.4327 and 0.8097. The posterior mean and standard deviation of the baseline risk standard deviation were 0.788 and 0.0911. When studies had zero cells for some data points, the ORs could not be calculated; thus, the trial results could not be included in the analyses.
Planned method of statistical analysis
After completing data extraction, a treatment map of all reported interventions was compiled for litter type, flooring type, and litter additives (Table 2). Litter types and flooring types that were similar were collapsed into the same intervention for the analyses. For the analyses of litter and flooring type, different depths or new versus used litter for the same litter type were collapsed into the same intervention category.
Table 2. Treatment map of litter type, floor type, and litter additive interventions for a systematic review and network meta-analysis of litter management strategies to prevent morbidity and mortality in broilers
All interventions related to litter type, flooring type, or litter additives contributed to the NMA. However, due to a large number of interventions and low amount of replication, NMA results are shown for single litter types and for additives that were evaluated in more than one study for mortality and the presence or absence of footpad lesions (the most commonly reported morbidity outcome).
The methodological approach for conducting NMA has been described in detail elsewhere (Dias et al., Reference Dias, Welton, Sutton and Ades2011; O'Connor et al., Reference O'Connor, Coetzee, da Silva and Wang2013). As the outcomes were binary, results presented as raw data or ORs were converted to the log OR. If the authors reported an RR, this was converted into a log OR using the reported risk of disease in the placebo group. If the authors reported the probability of an outcome in each intervention group based on a statistical model, that probability was converted back to logs OR, using a process described elsewhere (Hu et al., Reference Hu, Wang and O'Connor2019).
Selection of prior distributions for Bayesian analysis
The choice of prior probability distributions was based on an approach reported previously (Dias et al., Reference Dias, Welton, Sutton and Ades2011). Accordingly, we assessed both σ ~ U (0.2) and σ ~ U (0.5). The results suggested that σ ~ U (0.5) was preferred, and so we retained this prior in the model.
Implementation and output
All posterior samples were generated using Markov Chain Monte Carlo (MCMC) simulation, which was implemented using Just Another Gibbs Sampler (JAGS) software (Plummer, Reference Plummer2015). All statistical analyses were performed using R software (version 3.5.2) (R Core, 2015). The model was fitted using JAGS; JAGS was called from R via the rjags package (version 4-8) (Plummer, Reference Plummer2015). Three chains were simulated in the analysis, and the convergence was assessed using Gelman–Rubin diagnostics. We discarded 5000 'burn-in' model iterations and based all inferences on a further 10,000 model iterations. The model output included all possible pairwise comparisons using log ORs (for inconsistency assessment), RRs (for comparative efficacy reporting), and the treatment failure rankings (for comparative efficacy reporting).
Assessment of model fit
The fit of the model was assessed based on the log OR, by examining the residual deviance between the predicted values from the NMA model and the observed value for each study (Dias et al., Reference Dias, Welton, Caldwell and Ades2010).
Assessment of inconsistency
NMA relies on an assumption of consistency between direct and indirect intervention effects, apart from the usual variation that stems from a random-effects meta-analysis model (White et al., Reference White, Barrett, Jackson and Higgins2012). For example, if one trial compares the direct effect of a treatment A with the effect of treatment B, and another study compares the efficacy of treatments B and C, then the effect of A relative to B and B relative to C can be used to infer the (indirect) effect of A relative to C. The assessment of inconsistency compares whether the direct effects and the indirect effects give a similar result. We used the back-calculation method to assess the consistency assumption in our NMA (Dias et al., Reference Dias, Welton, Caldwell and Ades2010). We compared the estimates from the direct and indirect models and considered the standard deviation of each estimate, rather than relying on the P-values.
Risk of bias across studies (across the network)
A modification of the Grading of Recommendation Assessment, Development and Evaluation (GRADE) approach for NMA was used to describe the quality of the network (Salanti et al., Reference Salanti, Del Giovane, Chaimani, Caldwell and Higgins2014; Papakonstantinou et al., Reference Papakonstantinou, Nikolakopoulou, Rücker, Chaimani, Schwarzer, Egger and Salanti2018). The GRADE framework provides a method of evaluating the quality or certainty of evidence and the strength of the recommendations derived from that evidence. The GRADE for NMA was conducted using the Confidence in Network Meta-Analysis (CINeMA) online software program (http://cinema.ispm.ch). This platform uses a frequentist approach to calculating intervention effects, which is based on the meta-for package in R (Viechtbauer, Reference Viechtbauer2010). Thus, the contribution matrix of direct and indirect evidence produced for the risk of bias in CINeMA is based on a frequentist analytical approach. The GRADE approach in CINeMA evaluates the evidence network for the following domains: within-study bias, across-studies bias, indirectness, imprecision, heterogeneity, and incoherence. These domains were modified for this review by reporting the contribution of studies based on the reporting of randomization and the reporting of blinding of outcome assessors, rather than the domains of within-study bias and indirectness. The rationale was that randomization and blinding would have more variability among studies in this review compared to the overall risk of bias, and would therefore be more informative. In a GRADE assessment for NMA, indirectness refers to the differences between the populations, interventions, and outcomes in the included studies and the populations, interventions, and outcomes that were the target of the NMA (Salanti et al., Reference Salanti, Del Giovane, Chaimani, Caldwell and Higgins2014). We assumed that the trials included in this review would have minimal indirectness because we restricted the review to relevant populations. To characterize randomization as reported in each of the included studies, we sorted each trial included in the NMA into one of three categories as follows: (1) the authors reported random allocation to intervention groups and provided information on the method used to generate the random sequence, (2) the authors reported random allocation to intervention groups without providing information on how the sequence was generated, or (3) the allocation method was not random or no information on the method of allocation was provided. For blinding, we categorized the trials based on the following: (1) that outcome assessors were blinded, or (2) that outcome assessors were not blinded or no information on blinding was provided. The risk of bias instrument that was used for this review also included a question on whether animal caregivers were aware of the intervention allocation (i.e. were not blinded). However, with litter management, it would be difficult to blind caregivers to the intervention. Thus, we did not include blinding of caregivers as a component of this assessment of blinding.
The process required to assess across-studies bias in an NMA is not well developed. Additionally, no pairwise comparisons in this review included more than 10 trials, which is the number typically believed to be necessary for an accurate across-studies bias assessment (Sterne et al., Reference Sterne, Gavaghan and Egger2000). Thus, we did not access across-studies bias.
The assessment of imprecision in GRADE indicates whether the boundaries of the confidence intervals for the intervention effect fall within or between estimates that would be consistent with a clinically appreciable benefit or harm, or whether the intervention effects are clinically ambiguous (i.e. the confidence intervals span values representing both benefits and harms, or benefit or harm and a null value). We used an OR of 0.8 to represent a clinically meaningful difference. Thus, an appreciable benefit would correspond to an OR of <0.8 and appreciable harm would correspond to an OR of >1.25. ORs of 0.8 and 1.25 also were used in the assessment of heterogeneity because, for risk of bias assessment in an NMA, the major impact of heterogeneity is whether it will affect decision making. We did not present the results of the incoherence analysis from CINeMA, which measures the consistency of the network, because we presented the consistency analysis results based on the Bayesian analysis described previously in the methods section, rather than the frequentist method used by CINeMA.
A pairwise meta-analysis was conducted to compare the effect of fresh litter and re-used litter on broiler mortality. The pair-wise meta-analysis was conducted in R. 3.5.2 (R Foundation for Statistical Computing, Vienna, Austria) using RStudio version 1.1.463 (RStudio Inc., Boston, MA, USA) with the 'meta' package (Schwarzer, Reference Schwarzer2019). The meta-analysis used a random-effects approach, and inverse variance was used for weighting. Heterogeneity between studies was assessed using the I 2 statistic (Higgins and Green, Reference Higgins and Green2011; Schwarzer, Reference Schwarzer2019).
The flow of studies through the review process is shown in Fig. 1. Of the 1582 unique references identified in the search, 343 were assessed for eligibility at the full-text screening. Following the full-text screening, 126 eligible trials were identified in 103 publications, of which 97 trials in 76 publications had extractable data for one or more relevant outcomes; thus 97 trials were ultimately included in the review.
Fig. 1. Flow diagram of included studies for litter management strategies in broiler chickens.
The study characteristics, category of intervention, and outcome types are presented in Supplementary Table S1. Citation details for the studies described in this table are included as a Supplementary reference file. There were 17 countries represented at the trial level, although the country where the trial was conducted was not reported for 60 of the 97 trials. Of the trials where the country was reported, the most common country was the USA (n = 13 trials) followed by Brazil, Serbia, and Romania (n = 3 trials each). The studies were conducted in university or research flocks in 40 trials, in commercial flocks in 19 trials, and the setting was not reported for 38 trials. The month and year of during which the trial was conducted were not reported for the majority of the trials (n = 86/97). The number of farms included in each trial ranged from 1 to 4, with 85 trials conducted on a single farm. In terms of the interventions investigated in each trial, there were 50 trials that evaluated the type of litter, 22 trials that evaluated litter additives, 11 trials that evaluated litter depth, 10 trials that compared fresh to re-used litter (three of which also evaluated litter type or litter depth), six trials that evaluated flooring type, and one trial that evaluated windrowing (i.e. a form of partial composting in which litter is stacked, causing the internal temperature of the stacks to rise and thus destroy harmful pathogens).
Mortality was measured as an outcome in 82 trials (Supplementary Table S1). One or more morbidity outcomes were measured in 54 trials. Morbidity outcomes that were measured on a continuous scale included footpad lesion scores (n = 16 trials), breast lesion scores (n = 7), hock lesion scores (n = 5), gait scores (n = 2), leg lesion scores (n = 1); morbidity lesions measured dichotomously (i.e. presence/absence) included footpad lesions (n = 23 trials), breast blisters (n = 17), bruises (n = 3), leg lesions (n = 2), hock burn (n = 2), scabs (n = 1), and abnormal gaits (n = 1). Condemnations at slaughter were measured in two trials, and there were no trials evaluating total antibiotic use.
Results of individual studies included in the network
Due to a large number of trials and outcomes, the NMAs focused on interventions related to litter type, flooring type, or additives for the two outcomes for which there were the most data: mortality and a morbidity outcome, the presence or absence of footpad lesions. Not all of the trials that measured a given outcome were included in the NMA, because some intervention arms were collapsed into a single category which meant the trial was no longer comparative, and because some trials had zero cells which precluded the calculation of an OR. Of the 82 trials in which mortality was an outcome, 14 trials did not examine litter type, flooring type, or additives as an intervention (Supplementary Table S1), and four trials from three publications had zero cells for all intervention comparisons (Akpobome and Fanguy, Reference Akpobome and Fanguy1992; Hafeez et al., Reference Hafeez, Suhail, Durrani, Jan, Ahmad, Chand and Rehman2009; Avdalovic et al., Reference Avdalovic, Vucinic, Resanovic, Avdalovic, Maslic-Strizak and Vucicevic2017). Additionally, the intervention arms were collapsed into the same intervention based on the treatment map for three of the trials (Do et al., Reference Do, Choi and Nahm2005; Abreu et al., Reference Abreu, de Abreu, Jaenisch, Coldebella and de Paiva2011; Mendes et al., Reference Mendes, Paixão, Restelatto, Reffatti, Possenti, de Moura, Morello and de Carvalho2011). Thus, there were 61 trials included in the NMA for mortality as an outcome. None of the included trials included adjustment for flock (barn) effects.
Of the 23 trials that measured footpad lesions as a binary outcome, one trial had all zero cells (i.e. no birds had footpad lesions) (Sahoo et al., Reference Sahoo, Kaur, Sethi, Sharma, Chandra and Chandrahas2017) and all birds were positive for the outcome in another trial (Vargas-Galicia et al., Reference Vargas-Galicia, Sosa-Montes, Rodríguez-Ortega, Pro-Martinez, Ruiz-Feria, González-Cerón, Gallegos-Sánchez, Arreola-Enríquez and Bautista-Ortega2017); relative effect sizes could not be calculated in either case. There were five trials from four publications in which the intervention arms collapsed into the same intervention based on our treatment map (Petek et al., Reference Petek, Çibik, Yildiz, Sonat, Gezen, Orman and Aydin2010; Gholap et al., Reference Gholap, Macklin, Blake and Bilgili2012; Avdalovic et al., Reference Avdalovic, Vucinic, Resanovic, Avdalovic, Maslic-Strizak and Vucicevic2017; Shepherd et al., Reference Shepherd, Fairchild and Ritz2017). One trial had no intervention arms that also were reported in any other trial (i.e. the intervention arms in this trial did not link to the network) (Çavuşoğlu et al., Reference Çavuşoğlu, Petek, Abdourhamane, Akkoc and Topal2018), and therefore this trial could not be included in the comparative analysis. Thus, there were 15 trials from 12 publications included in the NMA for the presence or absence of footpad lesions. None of the trials included an adjustment for flock (barn) effects.
Risk of bias within studies by outcome
A summary of the individual study-level risk of bias for the 61 trials contributing to the mortality outcome NMA is shown in Supplementary Fig. S1. All studies included in that NMA were rated at a high risk of bias due to the method of allocating birds to different intervention or control groups: either the studies did not report the method used to generate the random sequence or the studies did not describe the method of allocation; and the studies also did not report whether there were any baseline imbalances. For three of the risk of bias domains, all of the trials were rated as 'some concerns' about the potential for bias, because in all cases, there was insufficient information reported in the studies to adequately assess the domains. First, none of the trials reported the blinding of outcome assessors, nor did any of the studies provide the additional information needed to assess whether there was the potential for differential management between groups. Further, none of the studies described whether any differences in management were balanced between intervention groups, which is related to the domain of bias due to deviations from intended intervention. Second, insufficient information was reported in each study to evaluate the potential for bias due to missing outcome data. Third, the risk of bias due to selected outcome reporting could not be assessed based on the available information, because assessing this domain requires a priori trial protocols to be available, and these are rare in the animal health literature. Another risk of bias domain, which concerns bias due to the outcome measurement process, was considered to be low in all trials in this NMA because mortality is objectively measured.
The results of the risk of bias assessment for the studies included in the NMA for the presence or absence of footpad lesions were similar to the results for the NMA with mortality as an outcome. All except one of the trials included in the NMA were judged to be at a high risk of bias due to the method of intervention allocation, and there were some concerns about the potential for bias due to deviations from intended interventions, missing outcome data, and the selection of the reported result. Unlike the mortality outcome NMA, all studies were rated as a high risk of bias under the domain related to the measurement of the outcome, as morbidity outcomes are more subjectively measured than mortality outcomes and outcome assessors were not blinded to the different intervention groups in any study.
Results of the network meta-analyses: mortality
There were 61 trials involving 129 comparisons that reported a mortality outcome and contributed to the NMA (Fig. 1). The geometry of the network is shown in Fig. 2, with the labels for the interventions defined in Table 2. There appeared to be two distinct clusters in the network: one primarily involving litter types that was centered around wood shavings as the type of litter, and the other primarily involving litter additives that was centered around aluminum as an additive. There was one comparison of two flooring types that was not connected to the network, meaning that there was no intervention arm in common with other published studies. The network for all intervention arms for which there were mortality outcomes with non-zero cells is shown in Fig. 3, with the number of comparisons for each intervention shown in parentheses beside the intervention node. Shavings and husks were the most common intervention arms, with 45 and 19 comparison arms, respectively.
Fig. 2. Geometry of the network of interventions related to litter type, flooring type, or litter additive and their effect on mortality in broiler chickens. Each circle represents an intervention, with a line between two interventions meaning that there was one more comparison between the interventions in the included literature. The key for the intervention acronyms is in Table 2.
Fig. 3. The network of intervention arms used in a network meta-analysis of the efficacy of litter management strategies to prevent mortality in broiler chickens. The size of the circle provides an indication of the relative number of intervention arms, the width of the lines provides a relative indication of the number of direct comparisons between interventions that were reported in the literature, and the number of arms for each intervention is shown in parentheses beside the intervention node. The key for the intervention acronyms is in Table 2.
Results of individual studies and synthesis of results
Although the NMA was informed by data from all intervention arms related to litter type, flooring type, or litter additives for which mortality was measured as an outcome, relative results for litter management options are presented only for single litter types or additives with multiple comparisons (aluminum and sodium bisulfate) due to the large number of possible comparisons. Comparative results are therefore presented for 15 litter types and two additives in the following ways: Fig. 4 illustrates the relative ranking of the litter management strategies for preventing mortality, with the 95% credibility intervals; the RRs for all pairwise comparisons are available in Supplementary Table S2; and the mean rankings are available in Supplementary Table S3.
Fig. 4. Forest plot for ranking of intervention arms for litter management options evaluating mortality as an outcome for litter management in broiler chickens. Mean rank and 95% credibility intervals are shown.
Based on the wide and overlapping credibility intervals on the relative rankings, there were essentially no differences in mortality risks among the litter management options. Additionally, the imprecision of estimation (indicated by wide credible intervals) for the relative risks means there is insufficient information to determine if there are differences in mortality risks across litter management interventions. These results are consistent with the distribution of the probability of failure (mortality) for each litter management option (see Supplementary Fig. 2).
Exploration of inconsistency
The measures of consistency between the direct and indirect comparisons of interventions with mortality as an outcome are presented in Supplementary Table S4. There was no evidence of inconsistency between the direct and indirect estimates because the credible intervals for all comparisons included the null value. However, as a result of the small number of studies contributing to each estimate, the confidence intervals were wide and the estimates were therefore imprecise.
Results of the network meta-analyses: presence or absence of footpad lesions
There were 15 trials with 30 comparisons that reported the presence or absence of footpad lesions as an outcome and contributed to the NMA (Fig. 1). The network for morbidity due to footpad lesions is shown in Fig. 5, with the number of comparisons for each intervention shown in parentheses beside the intervention node. The key for the acronyms is in Table 2. Similar to the network for mortality, wood shavings were the most commonly reported intervention, with many of the studies linking to this intervention arm. This suggests a non-random pattern of intervention comparisons in the literature, which is a researcher preference for including wood shavings as a comparator.
Fig. 5. The network of intervention arms used in a network meta-analysis of the efficacy of litter management strategies to prevent footpad lesions (measured as a dichotomy) in broiler chickens. The size of the circle provides an indication of the relative number of intervention arms, the width of the lines provides a relative indication of the number of direct comparisons between interventions that were reported in the literature, and the number of arms for each intervention is shown in parentheses beside the intervention node. The key for the intervention acronyms is in Table 2.
Although the NMA was informed by data from all intervention arms related to litter type, flooring type, or litter additives with foot pad lesions measured as a binary outcome, relative results for litter management options are presented only for single bedding types or additives with multiple comparisons (sodium bisulfate) for consistency with the mortality analysis. As a result, comparative results are presented for seven litter types and one additive.
Figure 6 illustrates the relative ranking of each litter management option for preventing footpad lesions, along with the 95% credibility intervals. RRs for the pairwise comparisons of the litter management options are available in Supplementary Table S5; the mean rankings are available in Supplementary Table S6. Peat moss had the highest estimated rank; however, this should be interpreted with caution because this was based on a single comparison. Because of the generally small number of comparisons for any given intervention, the credibility intervals on the rank estimates were wide and overlapping. Thus, there is no compelling evidence that any litter type is superior to the others. These results are consistent with the distribution of the probability of failure (presence of a footpad lesion) for each litter management option (see Supplementary Fig. 3).
Fig. 6. Forest plot for ranking of intervention arms for litter management options evaluating the presence or absence of footpad lesions as an outcome for litter management in broiler chickens. Mean rank and 95% credibility intervals are shown.
The consistency measurements between the direct and indirect comparisons for footpad lesions as a binary outcome are presented in Supplementary Table S7. There were a few comparisons where there was evidence of inconsistency between the direct and indirect estimates for several comparisons (n = 3/30), because the credible intervals did not include the null value. However, the comparison of the direct and indirect estimates for the remainder of the intervention comparisons indicated consistent results.
Risk of bias across studies
The results of the assessments of risk of bias across studies included in either the NMA for mortality or the NMA for the presence or absence of footpad lesions are shown in Supplementary Tables S8 and S9, respectively. For each outcome, there were a considerable number of comparisons in which there were major concerns about the potential for bias under the domain of imprecision, reflecting the large confidence intervals resulting from the small number of trials contributing to each comparison. There were few concerns about bias due to heterogeneity; this result was expected based on the wide confidence intervals on the RRs, again due to the small number of studies contributing to any given comparison.
The contribution of studies to the RR based on the approach to randomization for each comparison between the litter management options was calculated, and the results are shown in Supplementary Figs. S4 and S5 for mortality and footpad lesions, respectively. For both mortality and presence or absence of footpad lesions as outcomes, a visual inspection of the resulting figures illustrates that a substantive component of the evidence for many intervention comparisons had a high (red) or unclear (yellow) risk of bias due to the randomization process.
The contribution of studies to the RR for each comparison based on blinding is not shown for either outcome; there were some concerns about the possible presence of bias in all comparisons, as blinding of outcome assessors was not reported in any of the trials.
Additional analyses: pairwise meta-analysis
The results of the pairwise meta-analysis comparing mortality risk for broilers raised on fresh litter versus used litter are shown in Fig. 7. The meta-analysis included 12 comparisons from seven trials (Aggarwal et al., Reference Aggarwal, Singh, Gupta and Pal1978; Jones and Hagler, Reference Jones and Hagler1982; Malone et al., Reference Malone, Tilmon and Taylor1990; Malone and Gedamu, Reference Malone and Gedamu1995; Balogun et al., Reference Balogun, Adekeye, Olumeyan, Afolayan and Bawa1999; Vieira and Moran, Reference Vieira and Moran1999; Nunes et al., Reference Nunes, Scherer, Pozza, Eyng, Bruno and Vieites2012; Xu et al., Reference Xu, Stark, Ferket, Williams, Nusairat and Brake2015; Shepherd et al., Reference Shepherd, Fairchild and Ritz2017; Garcés-Gudiño et al., Reference Garcés-Gudiño, Merino-Guzmán and Cevallos-Gordón2018). The analysis indicated no association between fresh versus used litter on the risk of mortality (OR = 1.00, 95% CI = 0.84, 1.20), although there was considerable heterogeneity present (I 2 = 66%). There was no compelling evidence of small study effects based on a visual assessment of the funnel plot (Fig. 8).
Fig. 7. Pairwise meta-analysis of fresh versus used litter to prevent mortality in broiler chickens.
Fig. 8. Funnel plot for visual examination of small study effects from a pairwise meta-analysis of fresh versus used litter to prevent mortality in broiler chickens.
Summary of evidence
A considerable body of work addressed the topic of litter management to reduce morbidity and mortality in broiler chickens. The NMAs conducted in the present study did not indicate differences in mortality risks or footpad lesions for poultry exposed to different litter types, flooring types, or litter additives type. In addition, the pairwise meta-analysis did not find differences in mortality risk between poultry housed on fresh versus used litter. However, these results should be interpreted cautiously because of the small number of trials investigating each of the specific interventions, with the exception of the shavings and husks as the type of litter. It is important that trials be replicated in order to ensure that the results of investigations into the effects of interventions on given outcomes are consistent and accurate. Replication is an essential need for any research synthesis, be it expert opinion, narrative review, or meta-analysis. The advantage of a quantitative approach, such as meta-analysis or NMA, is that results can provide a visual representation, such as that in the figure of the ranking plots, of the uncertainty in a body of work that is not communicated if the same body of evidence is summarized either by expert opinion or narrative review.
Limitations of the body of research
In the NMA, similar interventions were combined to create intervention categories; for instance, the intervention category 'paper' litter included different densities of paper, recycled versus new paper, as well as chips, particles, and pellets (data not shown). The decision to combine similar interventions was because these variations in factors such as paper depth were not considered to represent truly different interventions, and because there would have been little or no replication of interventions without some combination into categories. However, combining similar but non-identical interventions does introduce heterogeneity into the definition of the intervention, and therefore potentially into the results of the NMA. For both the mortality outcome and the footpad outcome, there also was an intervention comparison that did not link into the network, meaning that these interventions were not common to any of the other intervention arms reported in the included trials. Researchers conducting future trials, and organizations funding research, could refer to the geometry and relative size of nodes in the networks developed in the present analysis to ensure that at least one of the interventions included in future trials are represented in the existing network. Additionally, researchers and research funders can use the information from the networks to target comparisons in future trials to address current gaps; this maximizes research efficiency by ensuring that future trials on litter management will build on the overall body of literature on this topic.
There was also considerable variation in the outcomes that were reported among trials, and in the ways in which the outcomes were measured (for example, using continuous versus binary measures). When synthesizing research results, continuous and binary outcomes cannot be combined, although it is possible to create a binary outcome from data presented on a scale if the value on the scale that corresponds to a zero score is identified. Variation in the outcomes that are reported across trials also reduces the power of evidence synthesis because different outcomes (e.g. breast blisters and footpad lesions) cannot be meaningfully combined in any research synthesis method. In the human healthcare literature, there are ongoing initiatives seeking to create core outcome sets for clinical trials that aim to address the heterogeneity in the outcomes reported between trials; an example is the COMET (Core Outcome Measures in Effectiveness Trials) initiative (Williamson et al., Reference Williamson, Altman, Bagley, Barnes, Blazeby, Brookes, Clarke, Gargon, Gorst, Harman and Kirkham2017). As examples, Wuytack et al. (Reference Wuytack, Gutke, Stuge, Mørkved, Olsson, Robinson, Vøllestad, Öberg, Wikmar, Mena and Smith2018) and O'Donnell et al. (Reference O'Donnell, Black, McCourt, McBrien, Clarke, Patterson, Blackwood, McAuley and Shields2019) provide examples of recent protocols for developing core outcome sets. Creation of a core outcome set requires the input of content experts who conduct trials in the target area to determine which outcomes are of critical importance. However, the establishment of core outcome sets does not restrict researchers from reporting other outcomes in their trial reports; rather, the intention is to provide guidelines for a minimum set of outcomes to be included in all trials. Including at least some common outcomes in all trials can help to maximize the value, comparability, and synthesis potential of trials addressing the same issue. Creating core outcome sets for studies evaluating interventions to improve health and thereby reduce antibiotic use in poultry may be warranted.
None of the studies included in the NMAs incorporated adjustments for non-independence of birds (i.e. clustering) within flocks. All of the data from the included trials were presented as raw numbers (i.e. number of birds with and without the outcome of interest and a total number of birds with intervention group) or as univariable measures of association, yet all of the birds were housed in groups (i.e. flocks or barns). Therefore, the stated sample sizes in the included studies do not necessarily correspond to the effective sample sizes included in the analyses. Statistical methods to control for clustering are available in most statistical packages. When appropriate control for clustering is not incorporated in an analysis, the resultant confidence intervals will be inappropriately narrow (Schukken et al., Reference Schukken, Grohn, McDermott and McDermott2003). The lack of control for clustering should be considered when interpreting the results of this NMA, i.e. if clustering were controlled, the imprecision would be even greater.
In the literature identified for this review, there were issues related to the quality of reporting in the trials, including the reporting of study characteristics as well as key design features related to the risk of bias domains. Concerns related to the quality of reporting of trials in livestock populations have been identified in studies designed to evaluate reporting practices (Wellman and O'Connor, Reference Wellman and O'Connor2007; Burns and O'Connor, Reference Burns and O'Connor2008; Sargeant et al., Reference Sargeant, Elgie, Valcour, Saint-Onge, Thompson, Marcynuk and Snedeker2009a, Reference Sargeant, Saint-Onge, Valcour, Thompson, Elgie, Snedeker and Marcynuk2009b; Winder et al., Reference Winder, Churchill, Sargeant, LeBlanc, O'Connor and Renaud2019). To address concerns about the quality of reporting, the REFLECT statement was developed by an expert consensus process to provide guidelines for the reporting of clinical trials in livestock populations (O'Connor et al., Reference O'Connor, Sargeant, Gardner, Dickson, Torrence, Dewey, Dohoo, Evans, Gray, Greiner, Keefe, Lefebvre, Morley, Ramirez, Sischo, Smith, Snedeker, Sofos, Ward and Wills2010a). The RELFECT statement includes a 22-item checklist describing which components of a trial should be reported, as well as an explanation and elaboration document that provides further explanation on the importance of each item (Sargeant et al., Reference Sargeant, O'Connor, Gardner, Dickson, Torrence, Dohoo, Lefebvre, Morley, Ramirez and Snedeker2010a). Examples of trials reporting each item in a comprehensive manner are also provided. The REFLECT statement methods and elaboration documents were co-published in multiple journals (O'Connor et al., Reference O'Connor, Sargeant, Gardner, Dickson, Torrence, Dewey, Dohoo, Evans, Gray, Greiner, Keefe, Lefebvre, Morley, Ramirez, Sischo, Smith, Snedeker, Sofos, Ward and Wills2010a, Reference O'Connor, Sargeant, Gardner, Dickson, Torrence, Dewey, Dohoo, Evans, Gray, Greiner, Keefe, Lefebvre, Morley, Ramirez, Sischo, Smith, Snedeker, Sofos, Ward and Wills2010b, Reference O'Connor, Sargeant, Gardner, Dickson, Torrence, Dewey, Dohoo, Evans, Gray, Greiner, Keefe, Lefebvre, Morley, Ramirez, Sischo, Smith, Snedeker, Sofos, Ward and Wills2010c, Reference O'Connor, Sargeant, Gardner, Dickson, Torrence, Dewey, Dohoo, Evans, Gray, Greiner, Keefe, Lefebvre, Morley, Ramirez, Sischo, Smith, Snedeker, Sofos, Ward and Wills2010d, Reference O'Connor, Sargeant, Gardner, Dickson, Torrence, Dewey, Dohoo, Evans, Gray, Greiner, Keefe, Lefebvre, Morley, Ramirez, Sischo, Smith, Snedeker, Sofos, Ward and Wills2010e; Sargeant et al., Reference Sargeant, O'Connor, Gardner, Dickson, Torrence, Dohoo, Lefebvre, Morley, Ramirez and Snedeker2010a, Reference Sargeant, O'Connor, Gardner, Dickson, Torrence, Dohoo, Lefebvre, Morley, Ramirez and Snedeker2010b) and also are available online (http://www.reflect-statement.org/; https://meridian.cvm.iastate.edu/). Although not specific to poultry, the REFLECT statement provides relevant reporting guidelines for any trials conducted in animal populations housed in groups. Using the REFLECT statement to guide the writing of trial reports will increase the ability of the readers of these reports to assess the external validity and the generalizability of the trial results to their population of interest. Compliance with REFLECT guidelines will also help the readers to evaluate the internal validity or potential for bias in the trial. Ultimately, this will increase the utility of the research being conducted on litter management options, as well as other animal health research.
Limitations of the review
It is possible that not all relevant literature was captured by our search, particularly given the vertical nature of the poultry industry and the likelihood of internal research being conducted by poultry companies that might not be made available in the public realm. We also included only English-language articles, and the exclusion of 12 non-English articles during the full-text screening may have biased our results. Other relevant non-English-language articles may have been missed by the search, which incorporated only English search terms. We also collapsed intervention arms to increase the power of the analysis, and this may have impacted the results, if, for example, beneficial and harmful litter types were collapsed into a common intervention arm. Nonetheless, this approach was deemed necessary, as was little exact replication of interventions among studies.
We reviewed the literature related to the impacts of litter management options on poultry health; these management options included litter type, flooring type, litter additives, litter depth, and the use of fresh versus reused litter. Although there was a large number of studies, there was considerable variation in the specific interventions that were assessed, as well as in the outcomes that were measured in different trials. NMAs of the impacts of litter type, flooring type, or additives on mortality and on the presence or absence of footpad lesions did not reveal large differences in the outcomes among the interventions, and the pairwise meta-analysis did not indicate significant differences in mortality risks between poultry exposed to new and used litter. These results were based on a small number of comparisons among most interventions. In many cases, the trials identified in this review did not report the study characteristics and of the trial design features related to the potential for bias. Well-reported trials using at least one intervention that has previously been reported in the literature, as well as reporting outcomes that are common in the literature, would help to grow the knowledge base on the potential impact of litter management on bird health.
The supplementary material for this article can be found at https://doi.org/10.1017/S1466252319000227.
JMS developed the review protocol, coordinated the project team, assisted with the data analysis, interpreted the results, and wrote the manuscript drafts. MdB, KC, KD, BD, JD, SM, CM, MR conducted relevance screening, extracted data, conducted risk of bias assessments, commented on manuscript drafts, and approved the final manuscript. DH conducted the data analysis, provided guidance for the interpretation of the results, commented on manuscript drafts, and approved the final manuscript. AMOC, CML, and AV assisted with the development of the review protocol, provided guidance on the interpretation of the results, commented on manuscript drafts, and approved the final manuscript. YS and CW provided guidance on the interpretation of the analysis and results, commented on manuscript drafts, and approved the final manuscript. CBW assisted with the development of the review protocol, assisted with data screening, data extraction and risk of bias assessment, conducted the analysis, provided guidance on the interpretation of the results, commented on manuscript drafts, and approved the final manuscript draft.
Support for this project was provided by The Pew Charitable Trusts.
None of the authors has conflicts to declare.
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Jan M. Sargeant (a1) (a2), Michele D. Bergevin (a1) (a2), Katheryn Churchill (a1) (a2), Kaitlyn Dawkins (a1) (a2), Bhumika Deb (a1) (a2), Jennifer Dunn (a1) (a2), Dapeng Hu (a3), Catherine M. Logue (a4), Shannon Meadows (a1) (a2), Carly Moody (a1) (a2), Anastasia Novy (a5), Annette M. O'Connor (a6), Mark Reist (a1) (a2), Yuko Sato (a6), Chong Wang (a3) (a6) and Charlotte B. Winder (a1) (a2)
DOI: https://doi.org/10.1017/S1466252319000227
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text:
Just 15 minutes up the coast from Nice, you'll find the 'Billionaire's Peninsula' Saint Jean-Cap-Ferrat, with some of the highest property values in the world. Its coastline is dotted with sumptuous estates hidden behind enormous gates and longer driveways, and most villas are only visible from the sea. But there are two fabulous villas that you can see, the illustrious Villa Ephrussi de Rothchild and the Villa Kerylos.
Getting to Both Villas from Nice
By Bus: Take Bus #15 Port de Saint-Jean from the Promenade des Arts bus stop located behind the Place Garibaldi Monoprix, or from the Port. It leaves every 20 minutes, and for Villa Kerylos get off at the Villa Kerylos stop (which is just after the Baie des Fourmis stop), and for the Villa Rothschild get off 6 stops later at Plage de Passable/Rothschild stop, which is just after the La Rade stop. For Sainto Sospir stay on until the last stop, the town of Saint Jean, and then it's a 20 minute walk.
Insider tip: I recommend going to Villa Rothschild first as it's much more of a wow and if you go early you might have the place all to yourselves.
By Train The Villa Rothschild is just a 10-minute walk from the Beaulieu train station (which you might recognize from the movie Dirty Rotten Scoundrels with Steve Martin and Michael Caine as a couple of Riviera con men.)
By Uber, it will cost approximately 20€ from Nice, and you will be driven in a comfortable chauffeured sedan by a professional driver.
Villa Ephrussi de Rothschild
After Beatrice, the Baroness of Rothschild, married into the ultra-rich Bank of France Ephrussi family, she needed a creative outlet. Upon seeing her husband's cousin's Greek-inspired Villa Kerylos, she decided to build her own Italian-style villa nearby. Combining her love of art and pink roses, she created and curated the villa and gardens herself, inspired by the grand Palazzios of Venice. A bit on the flamboyant side, often dressing in pink head-to-toe, she used her favorite flower and color as major motifs in her very personal creation.
The Villa: The full two floors of the opulent pink villa are open for your exploration, including Madame's private suite and boudoir. Perouse the Louis XVI furnishings, priceless paintings and sculptures, a celebrated porcelain collection, the tapestry room, the Fragonard salon, …and the surreal monkey room dedicated to her two beloved primate companions… did I mention that Baronnes Beatrice was a wee bit eccentric?
The Gardens: The sumptuous manicured gardens will make you feel as if you've just stepped into a Bronte novel, as you discover hidden gazebos, ancient statuary, the Temple of Love, pools and waterfalls, and secluded corners. The immense estate is covered with 9 lovingly cultivated rosaries and gardens including French, Florentine, Japanese, Spanish, and Exotic styles. If you are into horticulture, this is for you, and if you are around in early May, don't miss the Rose and Plant Festival at Villa Rothschild. (And for even more on Gardens in Nice, click here.)
The Dancing Fountains: The fountains are beautiful in their own right, but every 20 minutes they put on a show, with the steams and jets carefully choreographed to classical music.
The Tea Room: Open from 10am-7pm, the Tea Room offers a romantic spot for a light lunch or an elegant afternoon tea in an unforgettable atmosphere. A tea is less than 5€, but for 12€ you can pamper yourself with a 'thé gourmande', a tea and your choice of pastry.
The Villa by Night: Every Monday and Tuesday night from July through August, the Villa Rothschild opens its doors from 7:45pm-11:30pm, for an evening ambiance like no other. The villa is resplendent at night lit by hundreds of candles; enjoy live ballet and 'dancing fountains', wander the gardens with a 'picnic panier', enjoy the bar, or dine in the villa restaurant. Not cheap, but quite a unique experience. Tickets are 22€, or 47€ including a picnic basket to enjoy in the gardens, or 82€ including a glass of Champagne and dinner in the villa. There is also a family admission of 63€ for two adults and 2 children.
Spring and Autumn hours: 10am to 6pm
Summer hours: (July and August) from 10am to 7pm
Winter hours (November to February): winter weekends and holidays 10am to 6pm, but weekdays (Mon-Fri) open afternoons only, 2pm to 6pm. In winter the tea room is only open weekends.
Closed days: none! Open 365 days a year!
Guided Tours: Download the free app Ephrussi for an interactive guided visit. Otherwise, audio guides are included with admission, or private tours with a guide can be arranged.
Tickets: 16€ for adults, 15€ for seniors over 65, 11€ for children ages 7-25, 13€ for students of all ages with student ID, and there is also a family pass for 45€ which is good for 2 adults and 2 children under 25. Tickets are available at the door or online via the website.
The Villa Rothschild is included in the French Riviera Pass
Website: www.villa-ephrussi.com
Villa Kerylos
The two most famous villas on the coast have a family connection: The Baroness Rothschild's husband's cousin, Madame Fanny Reinach, built the luxurious Grecian Villa Kerylos in Beaulieu-sur-mer, and when the Baroness saw it she decided to build her own Italian-inspired Villa just two miles away, which I'm sure must have thrilled Madame Fanny no end.
Hours: Open 10am-5pm. Closed days: none! Open 365 days a year.
Tickets: 11.50€ for adults, and free for children and young adults under 25, and the disabled. Buy tickets and reserve your time online here.
The Villa Kerylos admission is included in the French Riviera Pass
Train: See above, and from the Beaulieu train station it is a 10-minute walk.
Bus: Again, see above; with Bus #15 Port de Saint-Jean get off at the 'Kérylos' stop, bus #100 get off at the 'Église' stop. You can see the Villa Kerylos on the far tip of the bay that starts at the Royal Riviera Hotel.
Uber: The address is Impasse Gustave Eiffel, Beaulieu-sur-Mer
Website: www.villa-kerylos.com
The 'Tattooed Villa' Sainto Sospir
Update: the villa is closed due to restoration and renovation until at least 2022.
If you want to see yet another villa, something completely different and a work of imagination and modern art, call ahead and make a reservation to see the 'Tattooed Villa' Sainto Sospir in Saint-Jean-Cap-Ferrat.
The fantastical Villa Santo Sospir is known as 'The Tattooed Villa' because Jean Cocteau methodically covered every surface with his boundless creativity. When a young socialite friend invited Jean Cocteau to spend a few days at her recently acquired villa, he came and …stayed for 11 years! Made restless by the newly painted white walls, he first made a drawing over the fireplace. Then he couldn't stop, eventually covering every surface in the villa. Click here for the full story by Margo Lestz, and click here for more on Jean Cocteau including his museum in Menton (also closed for renovation…)
How to get there: If you are coming back on bus 100, get off at the Pont Saint Jean stop and cross the street to transfer to bus #81, which comes every half hour; here's the schedule, but this bus only takes you half the way there, then it is a 25 minute walk. Or… walk down by the Royal Riviera Hotel to catch the gorgeous paved path to the Village of Saint Jean (15 minutes), and from there it is another 20 minutes to walk to get to the villa, which is on the tip of the presque-isle, near the Grand Hotel Cap Ferrat. Or just take an Uber
From Nice, take Bus #15 and stay on until the end, the village of Saint Jean. From there it is a 25 minute walk, or simply take an Uber. The address is 14 avenue Jean Cocteau.
Tickets and Hours: TBD when it reopens
Website: www.villasantosospir.fr
Back up to main History Museums page
Photo Credits: Villa Rothschild by Testus, Villa Kerylos by Christophe Recoura, and Villa Santo Sospir by Dina Sigtrix, all licensed under Creative Commons
Prehistoric Elephant Camp - Terra Amata and the Lazaret Cave

redpajama_set_name: RedPajamaCommonCrawl


The Board of Secondary Education, Rajasthan 3rd Grade Teacher posts notification is out. All the eligible candidates for Rajasthan Board of Secondary Education 3rd Grade Teacher (TSP) Level-II Vacancy can go through the details of 3rd Grade Teacher (TSP) Level-II Jobs and apply through the official site @ www.education.rajasthan.gov.in. Likewise the official notification for Board of Secondary Education, Rajasthan posts released at official website.
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NOT A HAMMER OF TRUTH EXCLUSIVE!!!1one! KE04 Power Structure: REVEALED!
I'd suggest someone do one for BC04, but we all know the Gay Marriage Amendment Committee is right next to Pat Robertson's office. And I'd gladly do one for the Badnarik/Campagna campaign (BC04 is already taken? WTF?), but I really don't know where they keep the UN demolition explosives.


text:
Onthophagus procurvus é uma espécie de inseto do género Onthophagus, família Scarabaeidae, ordem Coleoptera.

Foi descrita cientificamente por Balthasar em 1935.

Referências

Ligações externas

procurvus
Coleópteros descritos em 1935

redpajama_set_name: RedPajamaWikipedia