text:
16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria
G. S. Watts, K. Youens-Clark, M. J. Slepian, D. M. Wolk, M. M. Oshiro, G. S. Metzger, D. Dhingra, Lee D Cranmer, B. L. Hurwitz
Pharmacy Practice and Science
Genetics - GIDP
Statistics - GIDP
Biosystems Engineering, Research
Aims: Test the choice of 16S rRNA gene amplicon and data analysis method on the accuracy of identification of clinically important bacteria utilizing a benchtop sequencer. Methods and Results: Nine 16S rRNA amplicons were tested on an Ion Torrent PGM to identify 41 strains of clinical importance. The V1–V2 region identified 40 of 41 isolates to the species level. Three data analysis methods were tested, finding that the Ribosomal Database Project's SequenceMatch outperformed BLAST and the Ion Reporter Metagenomics analysis pipeline. Lastly, 16S rRNA gene sequencing mixtures of four species through a six log range of dilution showed species were identifiable even when present as 0·1% of the mixture. Conclusions: Sequencing the V1–V2 16S rRNA gene region, made possible by the increased read length Ion Torrent PGM sequencer's 400 base pair chemistry, may be a better choice over other commonly used regions for identifying clinically important bacteria. In addition, the SequenceMatch algorithm, freely available from the Ribosomal Database Project, is a good choice for matching filtered reads to organisms. Lastly, 16S rRNA gene sequencing's sensitivity to the presence of a bacterial species at 0·1% of a mixture suggests it has sufficient sensitivity for samples in which important bacteria may be rare. Significance and Impact of the Study: We have validated 16S rRNA gene sequencing on a benchtop sequencer including simple mixtures of organisms; however, our results highlight deficits for clinical application in place of current identification methods.
https://doi.org/10.1111/jam.13590
16S rRNA gene
bacterial identification
benchtop sequencer
Dive into the research topics of '16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria'. Together they form a unique fingerprint.
rRNA Genes Medicine & Life Sciences 100%
16S Ribosomal RNA Medicine & Life Sciences 99%
Bacteria Medicine & Life Sciences 64%
Ions Medicine & Life Sciences 39%
Metagenomics Medicine & Life Sciences 19%
Databases Medicine & Life Sciences 19%
Base Pairing Medicine & Life Sciences 16%
Watts, G. S., Youens-Clark, K., Slepian, M. J., Wolk, D. M., Oshiro, M. M., Metzger, G. S., Dhingra, D., Cranmer, L. D., & Hurwitz, B. L. (2017). 16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria. Journal of Applied Microbiology, 123(6), 1584-1596. https://doi.org/10.1111/jam.13590
16S rRNA gene sequencing on a benchtop sequencer : accuracy for identification of clinically important bacteria. / Watts, G. S.; Youens-Clark, K.; Slepian, M. J. et al.
In: Journal of Applied Microbiology, Vol. 123, No. 6, 12.2017, p. 1584-1596.
Watts, GS, Youens-Clark, K, Slepian, MJ, Wolk, DM, Oshiro, MM, Metzger, GS, Dhingra, D, Cranmer, LD & Hurwitz, BL 2017, '16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria', Journal of Applied Microbiology, vol. 123, no. 6, pp. 1584-1596. https://doi.org/10.1111/jam.13590
Watts GS, Youens-Clark K, Slepian MJ, Wolk DM, Oshiro MM, Metzger GS et al. 16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria. Journal of Applied Microbiology. 2017 Dec;123(6):1584-1596. doi: 10.1111/jam.13590
Watts, G. S. ; Youens-Clark, K. ; Slepian, M. J. et al. / 16S rRNA gene sequencing on a benchtop sequencer : accuracy for identification of clinically important bacteria. In: Journal of Applied Microbiology. 2017 ; Vol. 123, No. 6. pp. 1584-1596.
@article{7cec815afa2546869cf21064b62ae1e3,
title = "16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria",
abstract = "Aims: Test the choice of 16S rRNA gene amplicon and data analysis method on the accuracy of identification of clinically important bacteria utilizing a benchtop sequencer. Methods and Results: Nine 16S rRNA amplicons were tested on an Ion Torrent PGM to identify 41 strains of clinical importance. The V1–V2 region identified 40 of 41 isolates to the species level. Three data analysis methods were tested, finding that the Ribosomal Database Project's SequenceMatch outperformed BLAST and the Ion Reporter Metagenomics analysis pipeline. Lastly, 16S rRNA gene sequencing mixtures of four species through a six log range of dilution showed species were identifiable even when present as 0·1% of the mixture. Conclusions: Sequencing the V1–V2 16S rRNA gene region, made possible by the increased read length Ion Torrent PGM sequencer's 400 base pair chemistry, may be a better choice over other commonly used regions for identifying clinically important bacteria. In addition, the SequenceMatch algorithm, freely available from the Ribosomal Database Project, is a good choice for matching filtered reads to organisms. Lastly, 16S rRNA gene sequencing's sensitivity to the presence of a bacterial species at 0·1% of a mixture suggests it has sufficient sensitivity for samples in which important bacteria may be rare. Significance and Impact of the Study: We have validated 16S rRNA gene sequencing on a benchtop sequencer including simple mixtures of organisms; however, our results highlight deficits for clinical application in place of current identification methods.",
keywords = "16S rRNA gene, accuracy, bacterial identification, benchtop sequencer, sensitivity",
author = "Watts, {G. S.} and K. Youens-Clark and Slepian, {M. J.} and Wolk, {D. M.} and Oshiro, {M. M.} and Metzger, {G. S.} and D. Dhingra and Cranmer, {Lee D} and Hurwitz, {B. L.}",
note = "Funding Information: The authors thank Kelly Hoon and Matthew Dyer (both formerly of Ion Torrent) for helpful discussions; and Clarence Lee of Ion Torrent for providing the Ion Torrent Metagenomics kit. Library preparation and sequencing, other than for the 400 base pair V1–V2 amplicon, was performed by the Genomics Shared Service at the University of Arizona Cancer Center supported by the Southwest Environmental Health Sciences Center, NIEHS grant ES06694, and the Arizona Cancer Center, NIH grant CA23074. The V1–V2 400 base pair sequencing was performed by D.D. at Ion Torrent, Carlsbad, CA, USA. This work was supported by grants to B.L.H. from the Gordon and Betty Moore Foundation (GBMF4733 and GBMF4491) and assistance from the Arizona Center for Accelerated Biomedical Innovation (ACABI) at the University of Arizona. Publisher Copyright: {\textcopyright} 2017 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of The Society for Applied Microbiology.",
doi = "10.1111/jam.13590",
journal = "Proceedings of the Society for Applied Bacteriology",
T1 - 16S rRNA gene sequencing on a benchtop sequencer
T2 - accuracy for identification of clinically important bacteria
AU - Watts, G. S.
AU - Youens-Clark, K.
AU - Slepian, M. J.
AU - Wolk, D. M.
AU - Oshiro, M. M.
AU - Metzger, G. S.
AU - Dhingra, D.
AU - Cranmer, Lee D
AU - Hurwitz, B. L.
N1 - Funding Information: The authors thank Kelly Hoon and Matthew Dyer (both formerly of Ion Torrent) for helpful discussions; and Clarence Lee of Ion Torrent for providing the Ion Torrent Metagenomics kit. Library preparation and sequencing, other than for the 400 base pair V1–V2 amplicon, was performed by the Genomics Shared Service at the University of Arizona Cancer Center supported by the Southwest Environmental Health Sciences Center, NIEHS grant ES06694, and the Arizona Cancer Center, NIH grant CA23074. The V1–V2 400 base pair sequencing was performed by D.D. at Ion Torrent, Carlsbad, CA, USA. This work was supported by grants to B.L.H. from the Gordon and Betty Moore Foundation (GBMF4733 and GBMF4491) and assistance from the Arizona Center for Accelerated Biomedical Innovation (ACABI) at the University of Arizona. Publisher Copyright: © 2017 The Authors. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of The Society for Applied Microbiology.
N2 - Aims: Test the choice of 16S rRNA gene amplicon and data analysis method on the accuracy of identification of clinically important bacteria utilizing a benchtop sequencer. Methods and Results: Nine 16S rRNA amplicons were tested on an Ion Torrent PGM to identify 41 strains of clinical importance. The V1–V2 region identified 40 of 41 isolates to the species level. Three data analysis methods were tested, finding that the Ribosomal Database Project's SequenceMatch outperformed BLAST and the Ion Reporter Metagenomics analysis pipeline. Lastly, 16S rRNA gene sequencing mixtures of four species through a six log range of dilution showed species were identifiable even when present as 0·1% of the mixture. Conclusions: Sequencing the V1–V2 16S rRNA gene region, made possible by the increased read length Ion Torrent PGM sequencer's 400 base pair chemistry, may be a better choice over other commonly used regions for identifying clinically important bacteria. In addition, the SequenceMatch algorithm, freely available from the Ribosomal Database Project, is a good choice for matching filtered reads to organisms. Lastly, 16S rRNA gene sequencing's sensitivity to the presence of a bacterial species at 0·1% of a mixture suggests it has sufficient sensitivity for samples in which important bacteria may be rare. Significance and Impact of the Study: We have validated 16S rRNA gene sequencing on a benchtop sequencer including simple mixtures of organisms; however, our results highlight deficits for clinical application in place of current identification methods.
AB - Aims: Test the choice of 16S rRNA gene amplicon and data analysis method on the accuracy of identification of clinically important bacteria utilizing a benchtop sequencer. Methods and Results: Nine 16S rRNA amplicons were tested on an Ion Torrent PGM to identify 41 strains of clinical importance. The V1–V2 region identified 40 of 41 isolates to the species level. Three data analysis methods were tested, finding that the Ribosomal Database Project's SequenceMatch outperformed BLAST and the Ion Reporter Metagenomics analysis pipeline. Lastly, 16S rRNA gene sequencing mixtures of four species through a six log range of dilution showed species were identifiable even when present as 0·1% of the mixture. Conclusions: Sequencing the V1–V2 16S rRNA gene region, made possible by the increased read length Ion Torrent PGM sequencer's 400 base pair chemistry, may be a better choice over other commonly used regions for identifying clinically important bacteria. In addition, the SequenceMatch algorithm, freely available from the Ribosomal Database Project, is a good choice for matching filtered reads to organisms. Lastly, 16S rRNA gene sequencing's sensitivity to the presence of a bacterial species at 0·1% of a mixture suggests it has sufficient sensitivity for samples in which important bacteria may be rare. Significance and Impact of the Study: We have validated 16S rRNA gene sequencing on a benchtop sequencer including simple mixtures of organisms; however, our results highlight deficits for clinical application in place of current identification methods.
KW - 16S rRNA gene
KW - accuracy
KW - bacterial identification
KW - benchtop sequencer
U2 - 10.1111/jam.13590
DO - 10.1111/jam.13590
JO - Proceedings of the Society for Applied Bacteriology
JF - Proceedings of the Society for Applied Bacteriology


Today, I'm so happy to introduce to you Stephanie from The Crafty Kitty as part of the Shorts on the Line Sewalong! This is the first time I've ever had someone from the UK post on my blog and I kind of want you all to read this in a british accent, just because... haha! Stephanie has a really awesome organic fabric store and I'm drooling over this fabric right here, as well as, awesome art ideas, recipes, and overall a great attitude towards living a healthy lifestyle! I'm so glad to have her!
Well hello all you lovely shorts on the line-ers! I am really enjoying being a part of this sew-a-long, so thanks to Rachael whose fault it is who kindly invited me to take part, to Vanessa for allowing me to add my poorly punctuated writing to her blog and to Carla for being another lovely co-host! For those of you who don't know me, I am a stay at home mum to my two kids (3 and 1). I blog about sewing, kids crafts/activities and vegan cooking over at The Crafty Kitty! I also recently opened up an online organic fabric store and haberdashery, so if you are an eco-sewer, or just like cute fabric prints, please stop by and have a nosy! I'm still at that stage in my sewing, where I am not quite confident enough to draft my own pattern, but I really enjoy experimenting with existing patterns, so today I am sharing how I adapted one of my favourite patterns for cloth nappy wearing babies: The bubble pants from growing up sew liberated. I wanted a nice light fabric for these shorts, so I raided my upcycling box and found a wonderful green plaid Men's XL shirt, which in the UK is a 44-46 inch chest. It would have been even better if it was a 100% cotton shirt, for extra breathability, but in this case it was polyester/cotton blend.
Whenever I use shirt fabric to make clothing I like to remove the sleeves and collar, cutting close to the seams, giving you a nice expanse of fabric that lies nice and flat to make it easier to cut out your pattern pieces. I keep the cuffs and collars too and salvage buttons into my button jar!
With the bubble pants (and any other pattern that uses a single pattern piece for legs), I find the centre line of the pattern piece and line it up with the side seams of the shirt, to give a nice finish. I used the (US Size) 18-24 months pattern pieces and I started off cutting the leg piece a lot longer than the finished short. I wasn't really sure how much I was going to need to trim off and I figured it was best to have too much fabric than not enough! The pattern is made up of three pieces: a leg piece, front gusset and rear gusset. The leg piece I cut twice, remembering to reverse the pattern when I cut the second piece (phew!) and the front and rear gusset I cut just once from remaining shirt fabric. I put together the shorts as per the instructions until I got to the stage of finishing off the legs. The original pattern also uses a second lining layer so I did the whole finishing off raw edges, ironing open the seams and double stitching to give a nice finish. Originally I planned to make plain ungathered cuffs, but they looked horrendous, so on the spur of the moment I decided to add the original shirt cuffs.
I tried the shorts on my son and decided where I would like the cuffs to end on his little chubby legs. I took a few measurements and trimmed the short legs to a mere 1.75 inches (including seam allowance and measured on the inseam). Seriously, it is incredible how short you need to make clothing for a baby especially when they 'lose' a few inches due to how low a cloth nappy sits! I removed the shirt cuffs from the sleeves and trimmed the cuffs to approximately 2.25 inches which cut out the bulk of the original seam. I finished off the raw edges of both the legs and the cuffs with an overlocker stitch pinned the cuff (buttoned at the first button) to the leg and joined them together with a 0.25 inch seam allowance. As you can see from the photo, I had the short legs right way out and had the cuff on the outside right side facing the right side of the short leg and sewed the seam on the inside. The final leg length (measured on the inseam) was just 3.5 inches!!
Here are the finished cuffs. They sort of look like I made them from the original shirt sleeves, and well, if I had known that I was going to finish off the shorts like this, perhaps I would have tried to cut the pattern pieces from the sleeves! A thought for next time perhaps!
Sometimes, when you are messing around with a pattern, you get this far, try on the garment and it looks terrible (like these shorts did before I added the shirt cuffs), you wonder why you bothered "wasting all that time". Sometimes it works out perfectly (ah I love those moments) and sometimes you need to scratch your head and have that moment of inspiration. I really love how cute these little shorts look with the cuff and how they puff out!
I've been having so much fun making shorts this month (I made 3 different pairs so far) it is just such a shame that the weather over here in England has been anything but shorts weather! You can also find me rambling on Twitter and Facebook and I would love to hear about your upcycling ideas!


text:
Why flexible working is no longer just for the millenial workforce
16 Aug 16:00 by Laurie Jones
This month is has been reported that July 2019 was the hottest month ever recorded on Earth. Across the globe, the effects of the temperature could be felt. In Greenland, one of the most significant ice melts ever recorded poured 197 billion tons of water into the North Atlantic, raising global sea levels by 0.5 millimetres. Also, in Greenland and across the Artic, the heat sparked massive wildfires which produced CO2 emissions equal to those of all of Columbia in 2017. In Switzerland, rail tracks were painted white in the hope it would help to prevent them from buckling in the extreme heat. In the Netherlands, a local TV station broadcast nonstop images of wintry landscapes to help viewers forget the heat outside and in Paris, local officials set up neighbourhood "cooling rooms" where people could find air conditioning and cold water. Closer to home in the UK, London ground to a halt on the hottest day of the year on record as a huge number of train companies cancelled or delayed the majority of their services out of London. For many London commuters, the delays were up to 5 hours long in the afternoon, which combined with the extreme heat lead to a very uncomfortable journey for many. But, could this have been avoided?
The issue of climate change is huge but what could have been done on a smaller level to ease some of the pressure and discomfort felt by thousands of commuters? The first phrase that springs to mind is "flexible working". Often hailed as the buzzword of the millennial generation and associated with trendy start-ups and non-traditional working practices, isn't it time we all started using flexible working for its true purpose? In the lead-up to the heatwave, hundreds of Londoners were warned against travelling and yet, the trains were still heaving in the morning, despite the warnings. Is this because workers are required to work from their office? Or because of meetings that couldn't be rescheduled? Or is it just that their employers aren't yet offering flexible working?
The French Prime Minister, Edouard Philippe described how changes would have to be made to the French schooling system and working environments if these temperatures are going to be expected every year. Similar changes may need to happen in the UK where there is currently no law for the maximum temperature of a working environment. Many smaller or older offices and workplaces won't have air conditioning and as a result, sales of fans were through the roof. However, perhaps the UK needs to consider implementing such laws if we're going to continue to experience these types of temperatures year on year. The Trades Union Congress is also calling for the government to implement laws surrounding workplace temperatures and have proposed that employers must ensure that working environments do not exceed 30C degrees. An interim solution to all of this is to offer staff the flexibility to work from home on days when temperatures are so high. I think we can all agree that productivity is pretty low when we're faced with such uncomfortable conditions, particularly those unfortunate enough to have strict dress codes in place.
If the heat wave of July 2019 isn't enough to persuade you to offer flexible working, what about the huge number of statistics that highlight increased levels of productivity, motivation and general happiness and wellbeing from employees who are given the option to work flexibly? Or the major boost it can give to your employer brand when recruiting? Many potential employees have come to expect employers to offer some form of flexible working to enable them to have a better work-life balance.
Not only that, but we're currently experiencing the 4th industrial revolution of our time during an era of digitalisation, artificial intelligence and automated processes. For employees, this may mean that working remotely is easier than ever due to the rise in technology with smartphones allowing us to connect from anywhere and apps such as Skype enabling us to undertake meetings from anywhere in the world. Likewise, the benefits for employers are extensive, allowing them to recruit a global workforce, reduce office rent, scale at a quicker rate and increase productivity. The recruitment industry for example, is often considered to be very traditional in it's working practices with employees often required to work long hours during set times and often in corporate dress. With the rise in technology and the ability for consultants to 'meet' with candidates from anywhere, there seems less need for consultants to be tied to their desk at all hours of the day when their job can now be done far more remotely than ever before. This no doubt makes the industry seem much more attractive to potential new hires and gives employees the autonomy to work in a way that suits their lifestyle.
At PCR, we're currently trialling a new flexible working policy enabling employees to leave an hour earlier at the end of the day, providing they remain available over the phone and email during this time. The reason for this is that none of our employees live locally and the majority of staff have quite a long commute. By leaving earlier, we all get a better work-life balance whilst still ensuring that our clients and contractors receive service as normal. It also works well for screening candidates who are often only available in the evening after work. In addition, we all work from home one day a week to allow us all a little break from the commute! This day at home also enables us to schedule personal appointments without impacting on the rest of our working week. We've definitely noticed a greater level of satisfaction when it comes to work-life balance and often find that our days working from home are the most productive due to the lack of distraction from others.
If Flexible Working means happier, more comfortable and more productive employees and a reduction in costs for employers, then what's not to like? If this way of working appeals to you then get in touch as we're always looking for talented individuals to help us to grow our team.
2 months ago by Laurie Jones
4 months ago by Jane Abbott
Recruitment is broken - "We're different".
Technology Is Great But Face-to-Face Meetings Are More Valuable

redpajama_set_name: RedPajamaCommonCrawl


I am receiving more telephone and email enquiries about products. I'm loving the personal nature of these exchanges!
I am happy to receive orders in this way, even though it can be very time consuming, and I'm already running at full capacity. Making everything by hand is a time consuming affair… but it is a labour of love all the same!
And, I completely understand why not everyone can use the Wildasthewinds Etsy page… So, this is definitely the way forward.
*However, if you can use the Wildasthewinds Etsy page it'd be very much appreciated.
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Spiritual, moral, cultural and social learning
Our school has three key values which allow all pupils to understand their key role in ensuring success for everybody.
Our whole community are expected to understand what they can expect and what they are responsible for in order to ensure that everyone is respected, safe and able to learn. This is done in lessons, assemblies and in our conversations with pupils and their families around school.
Our Curriculum has a role in supporting our pupils as they develop holistically. Our school culture is one which recognises the whole child, as part of a family and we have a key role in preparing our pupils for their future, not just academically but in other ways too.
Spiritual learning- this is done in a variety of ways, through lessons such as RE and English, in assembly but also in appreciation of the arts and the natural world. Most of our assemblies contain a prayer, which pupils can respond to by saying Amen.( see RE and collective worship policy)
Moral learning- we endeavour to teach children about right and wrong through our school rules and in the way we work with our students.
Cultural learning- Pupils in our school are taught about the British Values of tolerance, mutual respect, rule of law, personal responsibility and liberty and democracy in a wide variety of ways . A key area which we know we need to pay extra attention is the area of tolerance for beliefs and cultures. Our geographical area is predominately white and Christian. Our pupils are rarely exposed to people from alternative backgrounds, faiths, creeds and cultures in their everyday life. We believe that tolerance can only come from understanding and as such we try to teach pupils about the wide variety of cultures within our country and around the world, as we can. Assembly, PSHE and RE lessons are central to this, as are opportunities to visit a multi-cultural city first-hand.
Visitors to school
There are regular visitors to school who assist with the delivery of our curriculum, these include local religious leaders, community readers, the Niscu theatre group, STEM science personnel and sporting coaches. Parents are also welcome to various events through the school year to help them understand our curriculum and to help them support at home.
We also have adhoc visitors to school who we use to support learning when required.
Trips, visits and off-site learning
Where funds and opportunity allow we are keen to take the opportunities presented by off-site learning. Through-out the year pupils engage in a range of trips and visits linked to the curriculum and we know that our pupils enjoy these and find them memorable.
Recent trips have included local walks around the village, science workshops, dance festivals and theatre trips.
Every year we take the whole school on a number of trips including a pantomime visit and summer term trip. Moreover the older pupils are offered a residential visit on an annual basis.
We believe that our pupils should all access their local environment as much as possible and in particular to get the opportunity to learn about their local history and the Lake District National Park. As such we explicitly state that all pupils should visit the Lake District at least once every year.
Our after-school club offer allows a supplement to the curriculum and can allow pupils to further develop their skills and interests.


text:
Backus Lake ist der Name folgender Seen in den Vereinigten Staaten:

 Backus Lake (Michigan), im Roscommon County
 Backus Lake (Minnesota), im Itasca County

redpajama_set_name: RedPajamaWikipedia


For the second year running, Finsbury Partner Dorothy Burwell has been named as one of the Top 100 ethnic-minority executives in the EMpower 100 Ethnic Minority Leaders List.
Published by the Financial Times, the EMpower report highlights and ranks 100 executives in the U.S. and UK who have worked to create an environment that brought the best out of black, Asian and minority ethnic employees. Dorothy also heads up the Natural Resources team in the UK and has been with Finsbury for 9 years.


text:
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Verizon Email On Iphone While you sync the iPhone and your computer for downloading iTunes as an example, you may discover that the programs surfaced. You may find many kinds of programs which provide the 1 contact dialing function to this iPhone. Theses apps are playing with substantial role inside the worldwide economy because they don't seem to be only for entertainment and fun, but a substantial amount of utility programs have been playing massive role in enterprise and in addition the worldwide market additionally.
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text:
Empire of Vampires 1.2.1 Richard
1.2 Richard
Even though vampires are much stronger than humans, they have similar weaknesses. Use the weaknesses to control them, not to destroy them. If I am right, they are not only the key to world domination, but most importantly to saving humanity.
From Richard Baxter's draft of "Letters to my Daughter" – Empire City 2035.
1.2.1 Meeting with Richard
*2033*
Selene looked from the window of her private jet over to the skyline of the city. For a hundred and twenty-five years she had been building a life according to her own ideas. And there were always good opportunities here. The flight from Hong Kong had been long, but she had used the time to prepare herself. With her flew about fifty of her slaves, whom she wanted to keep in her new life. Good staff was hard enough to find to replace them.
The first time she came here, she had to spend another week on a ship. Sometimes she wondered whether the Titanic would have arrived then, if she hadn't accidentally been on deck and discovered the iceberg. So she had warned the crew and they could change the ship's course in time. The ship had escaped with minor wounds and no one had died or had to fight for the lifeboats, which were far too short. Flying was much safer there she found.
She liked the city, but she had made it a principle to change continent after every marriage and to take on a new identity. She tried to remain inconspicuous, because not everyone had to know that she was immortal. Or often widowed. Very often. According to her papers, she was now twenty-eight again. And she was no longer called Kathrin but had taken her real name Selene.
Selene was already prepared for her next marriages: Twice a year she set up a new identity, so that whenever she needed a new one, a new identity was always in place. Women who were supposedly raised in one of the private schools she owned. The fun became more elaborate from time to time, but fortunately she had gathered enough financial resources to be able to afford it.
She had used the time of the flight to adapt to her new identity. Thanks to her complete control over her body she had grown a few millimeters and was now 1.85(6'1") meters tall. She had been big all her life, but not huge and every time she changed identity she grew to follow the growing humanity. In the last century, a few centimetres had already come together.
She had sacrificed her preference for leather clothing to a better material. Her long dead 24th husband from London had finally convinced her of latex in the eighties. And also her later husbands were crazy about touching her in latex. In the beginning she wore it for his sake, but in the meantime there were also high-quality clothes made of rubber, which since that time covered her body almost constantly. Her black latex catsuit was usually complemented by a corset and matching boots. Fortunately she didn't have to go through the airport controls like the common people.
Also her personal maids wore black latex, mostly even including a mask which only allowed breathing and seeing. Selene liked her slaves in rubber better. She liked the smell and the feeling on her skin. And with her subjects she liked that she could see much better through the latex uniform which feelings were hidden underneath. No distracting colors or unnecessary layers of air distorted the infrared image.
She hoped that the luxury would remain with her for a while, but it was foreseeable that all the states of the world would turn into surveillance states, which always knew where which citizens were. She had hated the cameras since Chicago, but hadn't had to kill for them for a long time. She had become much more careful. She practiced her gait and facial expression in front of the mirror again. And her voice. No computer in the world would still mistake her for Katherine or any of her former identities, but for her current identity Selene, which she had created twenty-eight years ago. She liked her real name and wore it again. Her next self with the name Selene was only five years old and would not be available for her for a long time.
Changing her fingerprints was by far the most painful part of the identity change. She had to hold her fingertips one by one in strong acid to completely dissolve the skin and then concentrate quite hard to get her new fingerprints to grow into the desired shape. It had taken a lot of practice to master the trick and she now managed to shape herself in the first attempt as intended, but she still didn't like it. But it was necessary to hide her immortality. Even though she had a good memory, when she was shaping the fingerprints she looked at a picture of the desired prints that were in her official files.
She wondered when the time would come for everyone to be equipped with chips that would record where they were around the clock. The technology seemed to be feasible, but it was still too expensive and the resistance was too high. But the invasion of privacy was not much bigger than taking fingerprints from babies. güvenilir bahis Selene sensed that time was running out for her. Eventually, no matter how much money she had, she couldn't hide anymore. It was time to finally start a family. And fulfill her destiny.
She was still able to keep up with the security features. Maybe she eventually learned how to change her iris scan, but Selene was afraid of the moment when she had to identify herself by means of her DNA or a chip she had to wear from birth.
Apart from the identity change, Selene had used the time of the flight to read. The twenty most important newspapers of the world, the complete bestseller list of the last half year, a few current patent applications, but mainly she read financial reports of companies that were potential investment objects. She read quickly and tried to keep up to date. After all, she intended to own this planet.
Selene had not been in town since the turn of the millennium, 33 years ago. And like last time, she came here looking for a new husband for her life. The last forty-one husbands had all been rich and handsome by human standards. But none of them had been able to resist her constant hunger for sex for long and had died of sexual overexertion no later than two years after the wedding night.
Selene had loved her last husband, as she had loved each of his predecessors, and had now mourned for nearly a year. Although she had a few lovers every day in between, she had not built up an emotional bond with any other lovers, only a culinary one. She had not been ready for more than blood, sex and sperm. Now she was ready to fall in love again and would possibly like sex so much that she too would have an orgasm. Only a few men, but all her female servants had the necessary skills to satisfy her.
Selene was compensated each time by a small fortune for the death of a husband, but meanwhile she was less concerned with money. She finally wanted children, but none of her husbands were able to inseminate her successfully. Neither could of her now thousands lovers, who all became her slaves after the first night of love and worked for her in her vast corporate empire for the rest of her life, making her even richer.
Selene knew that she liked women, but she didn't know how else to get pregnant. That's why she tried men, and when they saw more in her than a bedfellow and loved her sincerely, Selene returned the favor. She then fell in love with the man and married him. She still hoped that reciprocal love and frequent sex would increase her chances of pregnancy. At least her husbands managed to satisfy her more easily than normal food.
Selene had made a lot from her many inheritances, but not nearly as much as she could have. She always chose men who had no ties and were poor enough to keep them out of the public eye. And of course men who shared her current hobbies. The highest inheritance had earned her number thirty-seven with just over fifty million dollars thirteen years ago.
Most of her widely diversified assets of 373 billion had been acquired through skillful investments. And of course through the free labor of her former lovers and food donors. She was thus considerably richer than any human being, but she still managed to hide it. The fortune was officially divided among countless of her identities and foundations. Her effect on men was lasting enough that it was enough for about 14.000 slaves to be allowed to work for her and to give her food with their blood. But mostly Selene was generous and drank the blood at the source and did not use canned blood.
In her official identity she was an orphan, raised in a private school near London and inherited about fifty million from her parents. She did not want to attract attention, but she also did not want to live in a slum. Fifty million in official possession was OK there. She herself was a world traveler who had got to know the world after her medical studies. Of course, her studies had also been in one of her private universities, where the next generation was trained for her hospital chain. Sydney. She had chosen Hold as her last name, but she would give it up at the wedding in a few months anyway. Surely nobody noticed that her first name was the Greek goddess of the moon and, fittingly, her last name was the Hungarian moon. And if they did, she would tell, that her imaginary parents had chosen the first name to match the last name.
Actually, she had set herself a limit of a maximum of one hundred million as an asset for husband number forty-two. But here in the richest city in the world she could get twice as rich men without getting unwanted publicity afterwards, if she had to bury her husband a little later, completely unexpectedly, because of a heart attack. She did not want to become famous under any circumstances, that would only make the change of identity unnecessarily complicated.
Not that she killed on purpose. In fact, she usually refrained from sucking as much blood from her men as was medically justifiable. türkçe bahis Just small samples to make them compliant. She had loved each of her husbands, but each one had gone to great lengths to meet her high standards for good sex. And her weak human bodies did not endure for long. Only about a thousand times.
But even this limit of two hundred million would be significantly exceeded this time. She wanted Richard Baxter. Owner of Baxter Industries. The company consisted of an inherited construction company and a self-founded genetic engineering firm. The public estimated his assets at around thirty billion. Many believed he got rich off his high-tech company, but Selene knew better.
Richard Baxter not only owned Baxter Industries, but also controlled a few hotels with extra services. According to Selene's sources, he owned for about three thousand women, which worked as prostitutes for him. He also had his own organization of criminals who provided him with protection money and casino income. With all this illegal activity, Selene estimated his fortune at fifty billion. As far as she knew, he rarely killed. And when he did, he killed criminals worse than himself. The dead had rivaled him, even if not quite as successful as he was. So he got rid of them. She, too, had killed many times before, so she didn't blame him.
As always, Selene had used the time after her last husband's death to kill a few people. She focused on wanted terrorists, religious leaders, drug lords and corrupt politicians who, in her opinion, endangered humanity. She considered the killing of them as a service to society and as training. And after each of these murders her nightmares of a devouring fire became better. Helped even more than performing miracles in the hospital.
And the confiscated assets made her a little richer again. The last series of killings since the end of marriage forty-one alone had resulted in over five hundred deaths from ten organizations in just a few months. And Selene brought in an average of about a million and a half per death. The headlines were full of the end of the ten mafia cartels, but nobody suspected that she was involved.
At forty-seven years, Richard was still looking good. Trained, athletic and probably potent. At just over two Meters (6'8") tall, he would tower over her by half a head, despite possible high-heeled boots he seemed to be into. Selene had used the Titanic with his great-great-grandfather to cross the Atlantic Ocean. It was questionable whether, without her intervention on the ship, another human would have developed Richard's technology. Strange how life played out sometimes.
Richard had never married before and had no children. But he had a thing for women. Many of the female slaves in his brothels he had personally deflowered. So Selene didn't have to get rid of former wives.
But what was much more important to Selene than his looks and fortune: In addition to his construction company, he owned a fast-growing genetic engineering company with amazing success. His technologies allowed complete gene sequencing in only five minutes. And his company had patents to cure a dozen genetic diseases by repairing the genes. As far as she knew, he had made many of the inventions himself, adding an even more profitable genetic engineering division to his inherited construction company.
Perhaps he find out why her eggs absorbed sperm from men but were not fertilized by them. All Selene himself had discovered was, that she had not only twenty-three pairs of chromosomes, but twenty-eight. And her first twenty-three pairs, as far as she had found out, were human, only the five extra pairs of chromosomes made her a vampire.
Selene had put up a poster of him on the plane so she could get used to his appearance. She would have to love him and getting used to his looks was an important first step. At least he shaved and was trained. She gave him a kiss and got ready to take off.
Selene ordered her maids to dress her. She simply stood in the middle of the room and was to be rubbed with silicone oil by the three rubber beings. Being hairless helped, but with oil it worked better. Selene no longer dressed herself, it was much more comfortable to have staff to do it. The soft rubber hands on her body were very pleasant.
Next up was the tailor-made catsuit made of black latex. Today to stay inconspicuous no shiny latex, but dull. The suit had exactly the right size to build up the right exciting tension over the whole body. The integrated toe socks were a nice gimmick, but would soon disappear in the boots. The integrated gloves were also comfortable.
The suit had no opening or even zipper apart from the neck entry, but she was oiled, the suit from the inside too and the six practiced hands of her three maids had packed her very quickly and squeezed out all air bubbles. Very pleasant, especially in the crotch.
Selene's boots were flat and reached just below the knees. Also made of rubber, but more military than elegant. güvenilir bahis siteleri She wanted to jump off the plane with them and not go to the opera. The corset was also black, but much tighter than her usual corsets. Selene raised her arms, breathed out deeply and let herself be tied tightly until it could be closed at the back. The corset was quite heavy due to the steel struts, but also just strong enough so she couldn't accidentally destroy it if she breathed too hard. It was used to increase her fall speed and almost not being able to breathe was part of it.
Selene was paranoid and wanted to prevent her breathing from giving an infrared image. She was placed in the provided tub with ice cubes to cool her down and was not allowed to leave for a few minutes. She waited until her body had cooled down. She preferred to freeze than to be discovered. Only her lips occasionally came to the surface of the water so she could breathe.
After she was cold enough, the mask came on. She still had air for a few minutes and put on the mask of black rubber. The mask had only a few tiny holes in front of her eyes for her orientation, but no other openings. And also the holes in front of the eyes were only transparent latex and not permeable to air. There were no openings for mouth or nostrils. The mask also had an integrated rubber gag for noise reduction. Her next gasp would be down after landing. So that the mask collar would fit properly, they put a black steel collar on her, which was closed. One key remained in the plane, the other one was waiting for her downstairs.
She climbed down through a flap to the wheels in the already opened wheel well. She felt and heard the icy wind on her quick-drying rubber suit and concentrated on the jump. The plane didn't fly as precisely as she had hoped, but she was able to compensate for the hundred meter deviation to the south.
She saw below her the lights of the huge city, which was no longer growing wider as before, but was growing higher and higher. Even formerly rural suburbs were full of housing silos since the integration into the city. Forty million people. Every twentieth American lived in the largest city in the world. Everywhere heavy traffic and polluted air, but also full of opportunities. Selene saw the Hudson River and behind it the historic center on Manhattan Island. Selene got ready to jump off.
Their plane was on approach, but Selene wouldn't wait for them to land. Since airport security checks were equipped with body scanners three decades ago, she no longer felt safe doing so. She was only human on the outside. Any officer at the scanners would see that her bones were different. Different density. Ribs that were flatter, wider and more numerous. They would try to trap her and force her to kill hundreds of people. Or they could trap and dissect her, in case Selene didn't want to kill so many people.
Selene waited until the wheels of the plane were fully extended. She looked again briefly at the wind data and then jumped out of the plane under cover of darkness and rain clouds from a height of about one kilometer. To make sure she wouldn't be seen when she fell, she had chosen a rainy night and wore her black camouflage clothes. Despite the pattern of holes in front of her eyes she could see enough not to miss her house.
Jumping out of airplanes felt good. Like she was made for being in the air. Sometimes she wished she could fly, but she couldn't get up more than ten meters when she jumped. Selene knew she didn't need a parachute. She flew over the park and set her sights on her house. She just corrected her flight a little and a few seconds later landed with both feet in the middle of the roof of her 90th Street high-rise building. The trees on the roof had developed well over the last thirty years and it was like a small park on the roof terrace at a height of one hundred and eighty meters (590 foot).
Her servants were already waiting for her at the edge of the terrace and the highest-ranking one of them had the key in her hand. The maids wore a full-body suit made of black latex, similar to Selene's, but had neither boots nor corset on. Their mask had nostrils, but they also wore ball gag under the mouthless masks.
The three maids approached Selene and each one gave Selene a kiss on the mouth first. Strange but very intimate feeling for Selene to kiss a rubber ball through two layers of latex. While she was gagged and could not breathe. They also stroked through Selene's crotch with their hand. Selene was a predator and had to be put in a merciful mood if her bite was to be pleasant. Only when Selene relaxed, but also when she was running out of air, Selenes collar was opened and they took off her mask.
Selene took a deep breath and inhaled the scent of the maids. She needed a moment to get enough air again. The corset did not allow her to get air quickly. She smiled at her maids. Good staff and being dependent on them gave them the illusion that they meant something to their mistress. Selene had prepared herself for her servants, but Selene had not intended them to be more than tenderness, a few treats and sex. Exchangeable personnel, whose names she knew but would not use. Too many of her good and tender servants had already died of old age.


text:
Nalmefen je organsko jedinjenje, koje sadrži 21 atom ugljenika i ima molekulsku masu od 339,428 -{Da}-.

Osobine

Reference

Literatura

Spoljašnje veze

 -{Nalmefene}-

Алкохоли
Амини


text:
Applying to Study History at Oxford University
Tom, Keystone Tutor
View Our Oxbridge &
University Entrance Tutors
Keystone Tutor Tom read History at Christ Church College, Oxford, graduating in 2015. In the interview below Tom covers his inside experience of the application process, the best ways to prepare, and some insights into his work as a tutor helping students gain entry to some of the UK's top academic institutions.
Is there an Admissions Test for History at Oxford University?
There is an admissions test for all applicants applying to read History and its joint schools. The History Aptitude Test (HAT) is an hour long skills-based assessment, constructed around a primary source. Candidates are asked a single question which prompts them to offer interpretations of the extract, without using any outside knowledge. The HAT tests the candidate's ability to read a source critically but sensitively and to organise ideas coherently, precisely and imaginatively into a written answer.
You can read more about the History Admssions Test in this Keystone article.
How to Write an Effective Personal Statement for History at Oxford
The main purpose of a personal statement is to demonstrate enthusiasm and suitability for the subject you are applying for. The best way to show this is to write about what you have most enjoyed studying in school, in History, and in your other subjects. It is also sensible to write about the books you have most enjoyed reading, both as part of your course, and in your own time. Rather than simply list these books, you should offer critical interpretations of them. Some questions that you might think about are - What was the author's argument? Did you agree with it? If not, why not? And what did it prompt you to read subsequently?
What Format does the History Interview at Oxford take?
A number of candidates are invited, after the HAT test, to go to Oxford for interviews. The interview is an opportunity for your potential tutors to evaluate, through an assessment of your intellectual skills, your academic potential for study in the tutorial or small classes system. Most candidates will normally have two interviews, and more if they are invited to interview at another college as well. (This can happen if one college is oversubscribed). The format of the interview varies from college to college and tutor to tutor and depending on whether the applicant has applied to read History or History with a joint school. Interviews may focus on the candidate's personal statement, the topics they have studied in school, the written work they have submitted, an extract from a monograph or primary source or wider themes and debates about History as a discipline.
What Kinds of Questions will be asked at the Oxford Interview?
An interviewer may ask you about a book you have read and what interested you most about it. The best way to approach this line of questioning is to summarise the argument of the book and the themes you found most interesting. Be prepared to make an interpretation of your own and to defend it: interviewers are looking for an ability to form arguments, in writing and in tutorials.
Which Oxford College is Best for History?
No one college is better than another for History. Some colleges do not offer all History and joint schools courses however, so it is important to check before choosing which college you apply to.
What is it like studying History at Oxford? – Interview with Keystone Tutor Tom
Prior to joining Keystone, Tom read History & Politics at Christ Church, Oxford (2012-2015).
How long have you worked for Keystone?
I have worked with Keystone since graduating in 2015.
What do you like most about working with Keystone?
I enjoy working with students one on one in tutorials, building up relationships with clients over a long period of time and working on building up students' confidence. I also particularly enjoy working on pro-bono projects.
How did you find the application process for Oxford?
I found applying to Oxford challenging but stimulating. Once I understood the demands of the HAT I was able to enjoy reading the extracts and the freedom to be more original in my thinking and writing than A-Level allowed. I found the interviews difficult but fair and a close replica of the tutorial system in which I would later study.
What preparation did you do in advance?
I made sure that I was comfortable talking about the wider reading that I had described on my personal statement and I looked at one or two HAT papers, ahead of the assessment.
What was the Oxford interview like?
I had two interviews - one with four History tutors, another with two Politics tutors. Both were informal and conversation-led. In my History interview we discussed a topic I had read about in my own time - the personal rule of Charles I. In the Politics interview we discussed some broader, more philosophical questions surrounding the relationship between the state and the individual. We also looked at a chart displaying data from an election and I was asked some questions about what it showed.
What was it like studying History at Oxford? What did you enjoy most?
I enjoyed reading History & Politics at Oxford and I enjoyed the ability to specialise in particular interests after Prelims (including through the extended essay and thesis) and also the tutorial system, which gave me unrivalled opportunities to read essays and discuss the topics I was studying with leading experts.
What are your top tips for someone thinking about applying to read History at Oxford?
My main tip is to be yourself - write about what genuinely interests you on the personal statement and don't try to play games, as the system will normally catch you out. The application process is as much an opportunity for you to work out if you are suited to study at Oxford, as it is for Oxford to work out if you are suited to them.
Tutors for HAT Preparation
Please do get in touch with Keystone Tutors if you are looking for a tutor to support your History application for Oxford University. We offer University interview training, advisory support and we have a range of specialist tutors who can assist students approaching the HAT.
Complete guide to Oxford University Interviews
Preparing for HAT
View our University Entrance
Oxford & Cambridge University
A Guide to Oxbridge Admissions Tests
Is it getting harder to win a place at the best universities in the UK? In 2020 ... Oxford received more than 23,000 undergraduate applications for 3,300 places. Cambridge received more than 20,000 undergraduate applications for around 4,500 places.
How to choose an Oxford college?
Oxford has 39 colleges, Cambridge 31. You can leave it to chance and make an open application, but most people expect to direct their application to one of them. In some ways, it seems an unnecessary distraction. After all, no one presents Formula One drivers on the starting grid with a wine list expecting them to choose which champagne they want to be sprayed with on the podium.
Applying to UK Universities from Hong Kong
UK universities are incredibly popular with students from Hong Kong, and Keystone Tutors has extensive experience in helping these students to gain admission to their desired university. This invaluable guide covers key facts and tips about the application process, how to choose the right university and course, and the costs and fees involved.


text:
Q: Fail with the same heierarchy in one axis and where mdx query I create MDX query:
with member [DTime.TimeYearQuaterMonthDayTimestamp].[Times] as
     Aggregate({[DTime.TimeYearQuaterMonthDayTimestamp].[2013].[4].[11].[17],
               [DTime.TimeYearQuaterMonthDayTimestamp].[2013].[4].[11].[18]}
              )
SELECT {[DTime.TimeYearQuaterMonthDayTimestamp].[Year].[Month} ON 1,
       {Measures.Price} ON 0
  FROM [Sales]
 WHERE ([DEndAuction].[true],[DTime.TimeYearQuaterMonthDayTimestamp].[Times] )

But in exercise is throw exception:

Caused by: mondrian.olap.MondrianException: Mondrian Error:Hierarchy '[DTime.TimeYearQuaterMonthDayTimestamp]' appears in more than one independent axis.

Can you help me? How to improve this query that in exercise does not throw exception?

A: To explain the error message: MDX permits a dimension to be used several times when it is all within the same axis. For example, if you had dimensions called [A] and [B] then this is allowed:
SELECT {[A].whatever} ON ROWS, {[B].whatever} ON COLUMNS FROM [mycube]

This is not allowed:
SELECT {[A].whatever} ON ROWS, {[A].whatever} ON COLUMNS FROM [mycube]

The same applies to the WHERE clause, which is like another axis. You cannot use your [DTime] dimension on 1 as well as within the WHERE.
Some people create a virtual dimension called [DayOfWeek] with 7 members, or [Hour] with 24 members, in order to show a report with months versus days or months versus hours.

redpajama_set_name: RedPajamaStackExchange


This saint medal of Czestochowski Madonna is individually painted and fired in a kiln to the temperature of 750°C (1300°F). A solid 585/1000 gold frame is hand-made.
Each and every Czestochowski Madonna medal is individually painted and fired in a kiln to the temperature of 750°C (1300°F). A solid 585/1000 gold frame is hand-made, without using any machine, highlighting the distinctiveness of this delightful item. You can be guaranteed of a truly unique work of art. The rear side of medal Czestochowski Madonna bears Latin inscription ORA PRO NOBIS! which means PRAY FOR US!


text:
Mittelniederdeutsches Handwörterbuch ist der Titel eines mehrbändigen Wörterbuches der mittelniederdeutschen Sprache, das seit 1923 an der Universität Hamburg erarbeitet wird. Ihm voraus ging bereits 1888 ein von August Lübben begonnenes und nach dessen Tod von Christoph Walther vollendetes Mittelniederdeutsches Handwörterbuch.

Zweck
Das Wörterbuch dient vor allem drei Wissenschaftsbereichen:
 der Linguistik und Literaturwissenschaft,
 der Geschichtswissenschaft; ein Kernbereich ist dabei die Hanseforschung,
 der Kulturgeschichte, historischen Soziologie und Volkskunde.

Darüber hinaus kann das Wörterbuch auch als Informationsquelle von landeskundlich und sprachgeschichtlich interessierten Laien genutzt werden.

Quellen und Materialbasis
Das Archivmaterial bietet einen repräsentativen Querschnitt durch die vielfältigen Textsorten und Anwendungsbereiche mittelniederdeutscher Sprache. Die zusammengetragenen ca. 1.000.000 Belege umfassen nicht nur edierte Quellen, sondern berücksichtigen auch Originalmaterial aus Archiven und Bibliotheken. Sie gewähren mit einer geschätzten Gesamtzahl der zu realisierenden Lemmata von über 50.000 Einträgen Einblick in weitreichende sprachliche Ausbau- und Differenzierungsprozesse sowie Kultur- und Sprachkontaktphänomene und vertreten so einen wesentlichen Ausschnitt niederdeutscher Sprachgeschichte. Die lexikographische Erfassung des mittelniederdeutschen Wortschatzes trägt dabei auch der breiten thematischen, textsortenspezifischen, regionalen und zeitlichen Fächerung des Quellenmaterials Rechnung. Eine Vielzahl überlieferter Quellentexte aus weitgestreuten Anwendungsbereichen dokumentiert die zentrale Rolle, die der mittelniederdeutschen Sprache vom 13. bis 17. Jahrhundert zukam. Die Überlieferung zeigt eine ausgeprägte Funktionalisierung historischer Schriftlichkeit in der Dokumentation und Vermittlung von Alltags- und Sachwissen, in der weitgehend differenzierten städtischen Verwaltungsliteratur und im diplomatischen Schrifttum (z. B. religiöse Traktate; Kräuter- und Arzneibücher; Kaufmannsrechnungsbücher und Warenverzeichnisse; Kämmereirechnungen u. a. Verwaltungstextsorten; Stadtrechte; Hanserezesse usw.) ebenso wie in den Zeugnissen einer europäischen Literaturgeschichte (z. B. Reynke de vos, Narrenschiff).

Geschichte
Der Plan für die Erstellung eines niederdeutschen Wörterbuchs wurde 1923 am Lehrstuhl für niederdeutsche Philologie der Universität Hamburg gefasst. Beteiligt waren der damalige Lehrstuhlinhaber, Conrad Borchling, sowie Agathe Lasch, die federführend als Bearbeiterin und Herausgeberin war. 1923 wurde auch mit der Materialsammlung begonnen. Ab 1928 wurden erste Ergebnisse publiziert. Der Zweite Weltkrieg brachte die Arbeiten zum Erliegen; Agathe Lasch erhielt unter dem nationalsozialistischen Regime als Jüdin Berufsverbot und wurde 1942 deportiert und ermordet. 1953 wurden nach längerer Unterbrechung die Arbeiten wieder aufgenommen. Die Herausgeberschaft übernahmen nun Gerhard Cordes und Annemarie Hübner. Nach Cordes' Tod wurde Dieter Möhn 1987 Herausgeber, 2014 übernahm Ingrid Schröder die Herausgeberschaft. Seit 1956 werden wieder Bände des Wörterbuchs publiziert.

Publikationsstand
 Publikationsbeginn: 1928
 Band 1 (A – F/V) 1956
 Band 2 (G – R) 2004
 Band 3 (S – W), erscheint in Lieferungen
 Geplanter Abschluss ca. 2023 mit drei Bänden

Literatur

Ausgaben
 Mittelniederdeutsches Handwörterbuch, begründet von Agathe Lasch und Conrad Borchling, hrsg. nach Gerhard Cordes und Annemarie Hübner ab 1993 von Dieter Möhn und Ingrid Schröder, I–III/1, [Hamburg 1928–] Neumünster 1956–2007.

Zustands- und Arbeitsberichte
 D. Möhn: Mittelniederdeutsches Handwörterbuch zwischen Torso und möglichem Abschluss. Ein Zustandsbericht. In: Korrespondenzblatt des Vereins für niederdeutsche Sprachforschung 97 (1990), S. 5–12.
 D. Möhn/I. Schröder: Mittelniederdeutsches Handwörterbuch – Wiederaufnahme der Lieferungen. 2. Werkstattbericht. In: Korrespondenzblatt des Vereins für niederdeutsche Sprachforschung 99 (1992), S. 52–59.
 D. Möhn: Arbeitsstelle Mittelniederdeutsches Wörterbuch. In: Arbeitsberichte der im Fachbereich Sprachwissenschaften eingerichteten Arbeitsstellen und Zentren; Berichtszeitraum: 1. April 1989 bis 31. März 1992. Hrsg. v. Universität Hamburg, Fachbereich Sprachwissenschaften, Hamburg 1993, S. 38–41.
 D. Möhn: Arbeitsstelle Mittelniederdeutsches Wörterbuch. In: Arbeitsstellen und Zentren, Arbeitsberichte April 1992 – März 1994. Hrsg. v. Universität Hamburg, Fachbereich Sprachwissenschaften, Hamburg 1995, S. 31–34.
 D. Möhn: Arbeitsstelle Mittelniederdeutsches Wörterbuch. In: Arbeitsberichte der im Fachbereich Sprachwissenschaften eingerichteten Arbeitsstellen und Zentren; Berichtszeitraum: April 1994 bis März 1997. Hrsg. v. Universität Hamburg, Fachbereich Sprachwissenschaften, Hamburg 1997, S. 13–15.

Weblinks
 Arbeitsstelle Mittelniederdeutsches Wörterbuch an der Universität Hamburg

Einzelnachweise

Deutsches Wörterbuch
Niederdeutsche Sprache
Hanse


Beyond just a memorable logo, good branding increases the value of a company, provides employees with direction and motivation, and makes acquiring new customers easier. So what exactly is a brand? A brand represents the sum of people's perception of a company's customer service, reputation, advertising, and logo.
In short, your brand is the way your customer perceives you. It is critical to be aware of your brand experience and have a plan to create the brand experience that you want to have a good brand doesn't just happen it is a well thought out and strategic plan.
This session will show you how to build a brand, the elements you need to look at and how to apply it to the customer journey.
James Massey, of 25 years in marketing, has worked on the agency and client side in the Far East, South Africa and in the UK with companies such as BA, Aviva, Monarch airlines and First Great Western. He has worked on many brand programmes helping businesses define what they do and how that is applied across the business. He is currently working with the Institute of Marketing lecturing at Universities in the South West to help students understand how marketing works in the commercial world.


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Genomic Literacy, Education, and Engagement (GLEE) Initiative
The GLEE initiative will inform and unite K-16 students and educators, public and community-based groups, and healthcare professionals to enhance the integration of genomic information and technologies into healthcare - as well as into society more broadly.
International Summit in Human Genetics and Genomics
The International Summit in Human Genetics and Genomics is a five-year initiative (2016-2020) designed to help developing nations build and expand their knowledge base, infrastructure, systems and technologies in genetics and genomics. Each fall, researchers from abroad travel to the National Institutes of Health in Bethesda, Maryland for one month of in-person training at the National Human Genome Research Institute (NHGRI). The Summit helps them to understand the prevalence and basis of genetic diseases in their nations and to address these public health challenges. The 2020 Summit will be held on August 31 - October 1, 2020.
Precision Medicine Initiative Survey
Informing the development of the Precision Medicine Initiative.
Human Genome Exhibition
The Genome: Unlocking Life's Code traveling exhibit is on a nationwide tour at museums and science centers across North America to educate and inform the public about genomic science. The exhibit is a partnership of the National Human Genome Research Institute (NHGRI) and the Smithsonian Institution's National Museum of Natural History.
Genome Research Fund
The Genome Research Fund supports genetics and genomics research at the National Human Genome Research Institute (NHGRI).
Biomarkers Consortium - Sarcopenia as a Valid Biomarker for Identifying Individuals at Risk of Disability
Sarcopenia 2 seeks to establish evidence-based cut-points for muscle mass and strength and determine their predictive validity for clinically meaningful outcomes (such as mobility, fractures, hospitalization and death); evaluate relative strength as a discriminator for mobility limitation and incident disability; and explore the potential usefulness of sarcopenia as a clinical endpoint in randomized clinical trials.
Biomarkers Consortium - Establish Guidelines for Initial Diagnostic Criteria for "Sarcopenia with Clinically Important Weakness" and Associated Evidence for Treatment Benefit
The Sarcopenia 1 project launched in 2010 and aimed to establish the first evidence-based definition of sarcopenia (muscle weakness), which is still not recognized as a medical condition.
Biomarkers Consortium - Bone Quality Project
The Biomarkers Consortium's Bone Quality Project aims to evaluate and to identify biomarkers of bone strength and quality changes by analyzing pooled imaging and biochemical data from multiple clinical studies to allow definition of better clinical endpoints.
(-) Remove Genetics and Genomics filter Genetics and Genomics
(-) Remove Osteoporosis filter Osteoporosis
National Human Genome Research Institute (NHGRI) (4) Apply National Human Genome Research Institute (NHGRI) filter
National Institute on Aging (NIA) (4) Apply National Institute on Aging (NIA) filter
National Cancer Institute (NCI) (1) Apply National Cancer Institute (NCI) filter
National Center for Advancing Translational Sciences (NCATS) (1) Apply National Center for Advancing Translational Sciences (NCATS) filter
National Eye Institute (NEI) (1) Apply National Eye Institute (NEI) filter
National Institute of Dental and Craniofacial Research (NIDCR) (1) Apply National Institute of Dental and Craniofacial Research (NIDCR) filter
National Institute of Nursing Research (NINR) (1) Apply National Institute of Nursing Research (NINR) filter
National Institute on Deafness and Other Communication Disorders (NIDCD) (1) Apply National Institute on Deafness and Other Communication Disorders (NIDCD) filter
National Institute on Drug Abuse (NIDA) (1) Apply National Institute on Drug Abuse (NIDA) filter
National Institute on Minority Health and Health Disparities (NIMHD) (1) Apply National Institute on Minority Health and Health Disparities (NIMHD) filter
NIH Office of the Director (OD) (1) Apply NIH Office of the Director (OD) filter


text:
/**
 * Jakarta Bean Validation TCK
 *
 * License: Apache License, Version 2.0
 * See the license.txt file in the root directory or <http://www.apache.org/licenses/LICENSE-2.0>.
 */
package org.hibernate.beanvalidation.tck.tests.bootstrap;

import static org.testng.Assert.assertTrue;
import static org.testng.FileAssert.fail;

import java.lang.reflect.Constructor;
import java.lang.reflect.Modifier;
import java.util.ArrayList;
import java.util.List;

import jakarta.validation.Configuration;
import jakarta.validation.Validation;
import jakarta.validation.ValidationException;
import jakarta.validation.ValidationProviderResolver;
import jakarta.validation.ValidatorFactory;
import jakarta.validation.spi.ValidationProvider;

import org.hibernate.beanvalidation.tck.beanvalidation.Sections;
import org.hibernate.beanvalidation.tck.common.TCKValidationProvider;
import org.hibernate.beanvalidation.tck.common.TCKValidatorConfiguration;
import org.hibernate.beanvalidation.tck.tests.AbstractTCKTest;
import org.hibernate.beanvalidation.tck.util.TestUtil;
import org.jboss.arquillian.container.test.api.Deployment;
import org.jboss.shrinkwrap.api.spec.WebArchive;
import org.jboss.test.audit.annotations.SpecAssertion;
import org.jboss.test.audit.annotations.SpecVersion;
import org.testng.annotations.Test;

/**
 * @author Hardy Ferentschik
 */
@SpecVersion(spec = "beanvalidation", version = "3.0.0")
public class ValidationProviderTest extends AbstractTCKTest {

	@Deployment
	public static WebArchive createTestArchive() {
		return webArchiveBuilder()
				.withTestClass( ValidationProviderTest.class )
				.withClasses( TCKValidationProvider.class, TCKValidatorConfiguration.class )
				.build();
	}

	@Test
	@SpecAssertion(section = Sections.VALIDATIONAPI_BOOTSTRAPPING_VALIDATIONPROVIDER_PROVIDER, id = "b")
	public void testFirstMatchingValidationProviderResolverIsReturned() {
		ValidationProviderResolver resolver = new ValidationProviderResolver() {

			@Override
			public List<ValidationProvider<?>> getValidationProviders() {
				List<ValidationProvider<?>> list = new ArrayList<ValidationProvider<?>>();
				list.add( TestUtil.getValidationProviderUnderTest() );
				list.add( new TCKValidationProvider() );
				return list;
			}
		};

		Configuration<?> configuration = Validation
				.byProvider( TCKValidationProvider.class )
				.providerResolver( resolver )
				.configure();

		ValidatorFactory factory = configuration.buildValidatorFactory();
		assertTrue( factory instanceof TCKValidationProvider.DummyValidatorFactory );
	}

	@Test
	@SpecAssertion(section = Sections.VALIDATIONAPI_BOOTSTRAPPING_VALIDATIONPROVIDER_PROVIDER, id = "c")
	public void testByDefaultProviderUsesTheFirstProviderReturnedByValidationProviderResolver() {
		ValidationProviderResolver resolver = new ValidationProviderResolver() {

			@Override
			public List<ValidationProvider<?>> getValidationProviders() {
				List<ValidationProvider<?>> list = new ArrayList<ValidationProvider<?>>();
				list.add( new TCKValidationProvider() );
				list.add( TestUtil.getValidationProviderUnderTest() );
				return list;
			}
		};

		Configuration<?> configuration = Validation
				.byDefaultProvider()
				.providerResolver( resolver )
				.configure();
		ValidatorFactory factory = configuration.buildValidatorFactory();
		assertTrue( factory instanceof TCKValidationProvider.DummyValidatorFactory );
	}

	@Test
	@SpecAssertion(section = Sections.VALIDATIONAPI_BOOTSTRAPPING_VALIDATIONPROVIDER_PROVIDER, id = "d")
	public void testValidationProviderContainsNoArgConstructor() {
		ValidationProvider<?> validationProviderUnderTest = TestUtil.getValidationProviderUnderTest();
		try {
			Constructor<?> constructor = validationProviderUnderTest.getClass().getConstructor();
			assertTrue( Modifier.isPublic( constructor.getModifiers() ) );
		}
		catch ( Exception e ) {
			fail( "The validation provider must have a public no arg constructor" );
		}
	}

	@Test(expectedExceptions = ValidationException.class)
	@SpecAssertion(section = Sections.VALIDATIONAPI_BOOTSTRAPPING_VALIDATION, id = "f")
	@SpecAssertion(section = Sections.VALIDATIONAPI_BOOTSTRAPPING_VALIDATIONPROVIDER_PROVIDER, id = "e")
	public void testValidationExceptionIsThrownInCaseValidatorFactoryCreationFails() {
		ValidationProviderResolver resolver = new ValidationProviderResolver() {

			@Override
			public List<ValidationProvider<?>> getValidationProviders() {
				throw new RuntimeException( "ValidationProviderResolver failed!" );
			}
		};

		Validation.byDefaultProvider().providerResolver( resolver ).configure();
	}
}

redpajama_set_name: RedPajamaGithub


There's nothing like a grilled pork loin, sliced up to feed a crowd – perfect in sandwiches, wraps, salads, paninis, pastas & rice dishes. For melt-in-your-mouth pork – the secret is a marinade, a pouch of wood chips and a hot grill!
Flank steak is one of the easiest cuts of steak to prepare. Marinate in a little soy sauce, garlic and olive oil, sear over high heat, allow to rest and you have never tasted anything so delectable!
Get yourself a mound of fresh, gorgeously green leaves – to the Japanese, this is gomae, to the rest of us, this is the only way to consume spinach. I promise. Stick with this method, switch up the greens, switch up the sauce and start scarfing spinach like you're trying to turn green!
The perfect way to prepare salmon is on the grill – with a quick marinade of dijon, soy sauce, olive oil, garlic & blue agave – the secret is touch it as little as possible – once when you put it on, once when you flip it and once when you take it off!
So how to perfectly cook bok choy? Simple – separate the leaves from the stalks – and you've got yourself a pile of perfecty prepped bok choy. Start with the stalks for a couple of minutes and finish with the leaves and sauté just until wilted – flavour any way you like – asian flavours are classic, but all sorts of things will work – so don't pass by the bok choy, cause you're passing up a green worth getting to know!
Sushi refers to the rice, it does not mean raw fish so on that note, making sushi at home is easy and versatile because most anything can end up un a sushi roll – tuna salad, hard-boiled eggs, lettuce, carrots, red peppers, avocado, cucumber, imitation crab – the list goes on and on and on.


text:
Q: Word 2016: Continuous break causes page break Using Word 2016, I hit the problem "Section break causes an unexpected page break in Word" which is described here: https://support.microsoft.com/en-us/kb/292074
The MS KB says the KB applies through Word 2007. But I found the resolution also applies to Word 2016.
Is there a better way to do my documents to avoid this? Was a total PITA to fight for awhile, and then find that it is a documented issue.

A: Also, I find that the linked article explains it due to footnotes, but I find it occurring in a document that has no footnotes or endnotes. But does have 2 columns. In Word 2016 for Mac.

A: IN Word 2010, I used the fix MS suggests, compatibility layout footnotes like Word 97 etc, but it was still giving a next page break.
However, if I put the continuous break on a paragraph break and then make that paragraph hidden, success, i get a new section without a new page.
This way I can put different page headers while using continuous breaks without always needing a new page


Olymp Resort is located in the heart of a spa area, just 100m from the beach. Here, guests are welcomed with luxurious rooms equipped with kitchenette, bedroom and bathroom. On the top of the building, located is a beautiful terrace where one can get a tan and admire the panorama of Kołobrzeg. The restaurant in the resort features elegantly served dishes. The accommodation charge includes access to the dry sauna and steam room, indoor swimming pool and Jacuzzi.
Professional wellness and physiotherapy, guaranteed by the Kinesis Treatment Facilities and comfortable conditions ensure absolute contentment during the stay.
In addition, the facility is completely accessible to persons with disabilities.
This is the place to visit whatever the season.
The prices include an access to a swimming pool, sauna and jacuzzi with an 8%VAT inclusive. The prices do not include a spa fee ( PLN 3.50 PLN/person/ day).
A surcharge for a single room is PLN 30.00/person/day, an extra bed (for 1 person over 3 years of age with breakfast and dinner) is PLN 80.00/person/day.
A surcharge of PLN 100.00/person for a stay from 24.12 to 26/12. It is a one-off payment (not a daily charge). The surcharge includes: A Christmas Eve with traditional dishes + a small gift, Christmas dinners, participation in concerts and shows held at this time.


text:
Equinor presents its first Energy transition plan
édité le 19/04/2022 - Plus de news de "Equinor" - Voir la fiche entreprise de "Equinor"
Equinor has published its first Energy transition plan, which will be submitted for an advisory vote by shareholders at the company's 2022 Annual General Meeting 11 May. The plan provides an overview of how the company is progressing towards its 2050 net zero ambition through short-term actions and medium-term ambitions.
Equinor also proposes to amend its object-clause, which currently has a sole focus on petroleum. The new wording will introduce "energy", which is in line with the current strategy and the company's ambition to deliver energy that has lower – and eventually net-zero – emissions.
"To decarbonise society, we need to be effective agents of change in the energy transition. We have set a clear direction to apply our experience, competence and the financial muscle from oil and gas to new value creating sectors of the energy system. We are pleased to present the plan to our shareholders and look forward to continued dialogue on the necessary decarbonization journey," says Jon Erik Reinhardsen, Chair of the Board of Directors in Equinor.
"Our Energy transition plan is based on actions. We believe it demonstrates that we have the right strategy, ambition level, capabilities and track record to be a leading company in the energy transition while ensuring long-term shareholder value creation and competitiveness," adds Anders Opedal, CEO and President of Equinor.
In February the company strengthened its ambition level by including a net group-wide emission reduction of operated scope 1 and 2 emissions by 50% by 2030. Equinor aims to achieve 90% of this ambition through absolute reductions This demonstrates that the company is taking action to reduce emissions under its operational control in line with a trajectory consistent with the goals of the Paris Agreement and a 1.5-degree pathway.
Equinor's strategy is to continue to create long-term value while supplying reliable energy with progressively lower emissions. The Energy transition plan integrates key elements of Equinor's decarbonisation strategy with existing actions and ambitions. It includes information on capital allocation, policy engagement, risk and performance frameworks, and other enablers to deliver on the company's ambitions. The plan builds on Equinor's many years of experience, its strategy and strong track-record.
Equinor has developed the plan in close collaboration with key stakeholders and it draws on investor group frameworks, including the Climate Action 100+ Net Zero Company Benchmark. The plan is informed by climate science and scenarios from the International Energy Agency (IEA) and the Intergovernmental Panel on Climate Change (IPCC).
The energy transition is a massive, shared, global challenge that will require coordinated action. Equinor is committed to being a proactive and constructive partner with governments as they put in place the necessary policy frameworks for the market to move towards a lower carbon energy system.
The plan was made public 22 March 2022. Progress on the energy transition plan will be reported annually.
About Equinor
Equinor (OSE:EQNR, NYSE:EQNR), is an international energy company with a proud history. Formerly Statoil, we are 20,000 committed colleagues developing oil, gas, wind and solar energy in more than 30 countries worldwide. We're the largest operator in Norway, among the world's largest offshore operators, and a growing force in renewables. Driven by our Nordic urge to explore beyond the horizon, and our dedication to safety, equality and sustainability, we're building a global business on our values and the energy needs of the future.
Origine : Communiqué Equinor
Voir la fiche entreprise de "Equinor"
Les dernières news de "Equinor"
18/01/2023 - Equinor : Gas discovery in the Norwegian Sea
10/01/2023 - Equinor awarded 26 new production licences on the Norwegian continental shelf (NCS)
05/01/2023 - Equinor and RWE have agreed to work together to develop large-scale value chains for low carbon hydrogen
28/12/2022 - Equinor : The Njord field upgraded and ready for another 20 years
21/12/2022 - Equinor : High gas exports and emissions cuts from Hammerfest LNG
16/12/2022 - Equinor : Krafla to be developed using ground-breaking concept
12/12/2022 - Equinor : More gas to Hammerfest LNG: Askeladd on stream
06/12/2022 - Equinor: Verlande securing higher production on the Norne vessel
01/12/2022 - Equinor : Plans for increased gas extraction from Oseberg approved
22/11/2022 - Equinor : Irpa, more gas to Europe and extended life for Aasta Hansteen
08/11/2022 - Equinor and Hitachi Energy sign a strategic collaboration agreement to accelerate the energy transition
14/10/2022 - Equinor : Peregrino phase 2 on stream
26/09/2022 - Equinor and Poland's PGNiG agree long term gas sales contract
02/09/2022 - Equinor completes exit process from Russia
31/08/2022 - Equinor : Turning North Sea projects into power in offshore wind
30/08/2022 - Equinor and Wintershall Dea partner up for large-scale CCS value chain in the North Sea
29/08/2022 - Equinor : Important step to decarbonise Europe
12/08/2022 - Equinor's H2H Saltend selected to proceed as one of the first large-scale hydrogen projects in the UK
20/07/2022 - Equinor restarts Peregrino field production
12/07/2022 - Equinor acquires energy storage developer in the US
06/07/2022 - Equinor : Strike-affected fields resuming production
05/07/2022 - Strike affecting Equinor-operated fields on the Norwegian continental shelf
04/07/2022 - Equinor : Fire at Mongstad refinery extinguished
30/06/2022 - Equinor transfers operatorship for Gulf of Mexico field to Shell
29/06/2022 - Fluxys and Equinor launch solution for large-scale decarbonisation in North-Western Europ
17/06/2022 - Equinor and partners consider 1 GW offshore wind farm off the coast of Western Norway
16/06/2022 - Equinor agrees winter gas supplies with UK's Centrica
15/06/2022 - Equinor joins the Mærsk Mc-Kinney Møller Center for Zero Carbon Shipping
10/06/2022 - Equinor signs long-term LNG purchase agreement with Cheniere
09/06/2022 - Equinor : New discovery near the Johan Castberg field in the Barents Sea
07/06/2022 - Equinor : Transocean Spitsbergen to drill for two licences
02/06/2022 - Equinor : Production start-up at Hammerfest LNG
01/06/2022 - Equinor completes acquisition of interest in the Statfjord field
26/05/2022 - Equinor exits all Joint Ventures in Russia
25/05/2022 - Equinor : Gas and condensate discoveries to be developed in the Norwegian Sea
16/05/2022 - Equinor : Hammerfest LNG to resume operations in a week
10/05/2022 - Equinor sells assets in Ekofisk Area and share in Martin Linge
06/05/2022 - Equinor : Extending contracts for drilling and specialist services
21/04/2022 - Petrobras and Equinor start production on IOR project at Roncador, Brazil
19/04/2022 - Equinor presents its first Energy transition plan
11/04/2022 - Equinor : New oil and gas discovery by the North Sea Troll and Fram area
07/04/2022 - Equinor and Naturgy team up to explore offshore wind development in Spain
05/04/2022 - Equinor awarded the Smeaheia and Polaris CO2 licenses
16/03/2022 - Equinor : New measures to maintain high gas exports to Europe
15/03/2022 - Equinor to stop trading in Russian oil and oil products
04/03/2022 - Equinor and bp sign agreement to transform South Brooklyn Marine Terminal into central hub for offshore wind industry
28/02/2022 - Equinor to start exiting from Joint Ventures in Russia
16/02/2022 - Equinor : Extending drilling contracts on the Norwegian continental shelf
01/02/2022 - Equinor : Wisting impact assessment
Toutes les news de "Equinor"