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Studies of liver tissue identify functional gene regulatory elements associated to gene expression, type 2 diabetes, and other metabolic diseases
Marco Cavalli1,
Nicholas Baltzer2,
Gang Pan1,
José Ramón Bárcenas Walls1,
Karolina Smolinska Garbulowska2,
Chanchal Kumar3,
Stanko Skrtic3,
Jan Komorowski2,4 &
Claes Wadelius1
Genome-wide association studies (GWAS) of diseases and traits have found associations to gene regions but not the functional SNP or the gene mediating the effect. Difference in gene regulatory signals can be detected using chromatin immunoprecipitation and next-gen sequencing (ChIP-seq) of transcription factors or histone modifications by aligning reads to known polymorphisms in individual genomes. The aim was to identify such regulatory elements in the human liver to understand the genetics behind type 2 diabetes and metabolic diseases.
The genome of liver tissue was sequenced using 10X Genomics technology to call polymorphic positions. Using ChIP-seq for two histone modifications, H3K4me3 and H3K27ac, and the transcription factor CTCF, and our established bioinformatics pipeline, we detected sites with significant difference in signal between the alleles.
We detected 2329 allele-specific SNPs (AS-SNPs) including 25 associated to GWAS SNPs linked to liver biology, e.g., 4 AS-SNPs at two type 2 diabetes loci. Two hundred ninety-two AS-SNPs were associated to liver gene expression in GTEx, and 134 AS-SNPs were located on 166 candidate functional motifs and most of them in EGR1-binding sites.
This study provides a valuable collection of candidate liver regulatory elements for further experimental validation.
The understanding of the genetics behind the molecular mechanisms involved in many liver and metabolic diseases remains elusive. Genome-wide association studies (GWAS) of diseases and phenotypic traits have been effective in finding association to gene regions but not the functional SNP(s) or the gene(s) mediating the effect [1]. This is likely due to heterogeneity within and between the study groups, for example, due to several functional common variants on a haplotype, common causative variants that differ between populations, or the contribution of rare personal variants [2]. Another major obstacle is the need to study the regulatory mechanism in the proper tissue.
To date, tens of thousands of associations have been reported between variants and diseases [3], but the question arises whether for example the association of a SNP to breast cancer has a biological relevance if it is located in a regulatory element observed in the skeletal muscle. Moreover, gene regulatory mechanisms are often studied in cell lines derived from cancer cells, which have single nucleotide and copy number variants that drive cancer as well as additional genetic aberrations acquired during prolonged culturing. In addition, culturing cells in the lab also changes the expression of many genes and may activate regulatory elements rarely used in physiological conditions or inactive elements with variants driving associations.
The majority of the GWAS top associated variants are located in non-coding regions [4, 5] and often in high linkage disequilibrium (LD) with several other variants, making it difficult to pinpoint the real functional SNP(s). One way to find putative functional variants is to detect regions with allele-specific (AS) binding of transcription factors (TFs) or their surrogate histone modifications, suggesting a different regulatory downstream role based on the individual genotypes. ChIP-seq data for TFs and histone modifications provide snapshots of direct and indirect protein-DNA interactions allowing the identification of heterozygous SNPs with significant allele-specific signals (AS-SNPs).
Here, we present the results of the identification of AS-SNPs using a minimal set of ChIP-seq datasets produced for two histone modifications and one genome architectural protein in human liver tissue, providing a collection of liver-specific candidate regulatory variants for experimental validations.
Figure 1 represents the blueprint of this study where we used ChIP-seq data and genomic sequence of a human liver sample to search for AS-SNPs. Initially, the diploid liver genome was reconstructed from the whole genome sequencing SNP calls which yielded a total of 4,588,678 SNPs, 97.8% of which were phased. Two million three hundred thirty-one thousand two hundred two heterozygous SNPs were used to create two personal genomes by replacing the reference bases at heterozygous sites with the alternative alleles. The use of linked-reads in the sequencing step allowed to maintain the correct phasing ensuring the high quality of the two genomes (see the "Methods" section). Using our established bioinformatics pipeline [2], the ChIP-seq reads from CTCF, H3K4me3, and H3K27ac were aligned to the personal genomes to identify heterozygous sites with allele-specific signals and the results were corrected for genome-wide testing. We selected these two established histone modifications which mark active enhancer elements or gene promoters that could act as enhancer elements since most of the reported disease-associated variants in the GWAS catalog are located in non-coding enhancers. CTCF was chosen as an architectural protein involved in the topological organization of the genome. CTCF-binding sites act as "anchor" points for the cohesin complex, and AS-SNPs flagged by a biased alignment of ChIP-seq reads from CTCF could reflect a regulatory activity potentially affecting the structural organization of the genome. We have previously found many allele-specific signals for these histone marks and CTCF in studies of cell lines.
Schematic representation of the study design. Genomic DNA from a human liver sample was submitted to whole genome sequencing (WGS), and the resulting variant calls were used to reconstruct a phased diploid version of the genome and from there two personal genomes (G1 and G2) by replacing the reference bases at heterozygous sites with the alternative alleles using the ALEA software [6]. Chromatin from the liver was used to perform ChIP-seq experiments, and the resulting enriched DNA sequences (purple rods) were sequenced and realigned to the two personal genomes using the ASAP software (http://www.bioinformatics.babraham.ac.uk/projects/ASAP/)
In order to minimize the false positives, we filtered out AS-SNPs located in blacklisted regions from the Encyclopedia of DNA Elements (ENCODE) project or in highly duplicated regions such as those in close proximity to centromeres or telomeres. The liver-specific collection of AS-SNPs obtained in this way consisted of 2329 unique heterozygous SNPs (Additional file 6: Table S2).
Associations of AS-SNPs to liver-related diseases and gene expression
The AS-SNPs identified by an allele-specific realignment of the ChIP-seq reads define putative regulatory elements that are likely to explain associations to disease in GWAS and to expression in studies of expression quantitative trait loci (eQTLs) (see the section "Genomic features" in the "Methods" section). Therefore, we selected 290 traits/diseases reported in the GWAS catalog and associated to liver activity and metabolism (Additional file 5: Table S1) and the selected AS-SNPs were intersected with the SNPs with the strongest association (GWAS top hit) and with SNPs in high LD (r2 > 0.8) with GWAS top hits. We identified 25 unique AS-SNPs associated to liver- and metabolic-related traits at 17 different genomic loci (Table 1 and Additional file 7: Table S3) providing new starting points to investigate environmental and gene regulatory signals at these GWAS-defined loci to further clarify the molecular pathways.
Table 1 AS-SNPs detected associated to liver-specific GWAS traits
For instance, we identified 4 AS-SNPs at two genetic loci associated to type 2 diabetes (T2D) on chromosome 6 and 17. In both cases, the allele-specific signals identified variants that are likely to better explain the associations observed in GWAS.
On chromosome 6, two AS-SNPs, rs655185 and rs541091, are in LD with the T2D-associated GWAS SNP rs622217. All three SNPs are reported by Genotype-Tissue Expression (GTEx) as eQTLs for the SLC22A3 gene in the liver, lung, testis, skin, brain, and esophageal mucosa. However, the analysis of the genetic background revealed that the GWAS SNP rs622217 is not located in a regulatory element due to the lack of transcription factor binding sites (TFBSs) while the two AS-SNPs are located in a regulatory element defined by several TFBSs from ChIP-seq in liver cell lines from the ENCODE project (Fig. 2). The two AS-SNPs are located in the first intron of the SLC22A3 gene, which encodes for a polyspecific organic cation transporter in the liver, kidney, intestine, and other organs also involved in the uptake of drugs like quinine and metformin, a known first-line medication for T2D. They are also located upstream of the LPA and PLG genes involved in the regulation of the fibrinolysis and atherosclerosis which have also been linked to T2D and familial hyperlipidemia.
UCSC Genome browser view of the AS-SNPs rs655185 and rs541091 (yellow) which are in high LD with the T2D-associated GWAS SNP rs622217 (cyan). The histone modification tracks represent the peaks called for the ChIP-seq of H3K4me3 performed in the liver (black) and the HepG2 signal from the ENCODE project (pink trace) as a comparison. Transcription factor binding sites (TFBSs) from ChIP-seq data from the ENCODE project for HepG2 and other cell lines. In the bottom insert, eQTL box plots from the GTEx project for the three SNPs with rank-normalized gene expression in liver tissue as a function of the different genotypes
These two AS-SNPs belong to a subset of 9 AS-SNPs (Additional file 8: Table S4) we identified in LD with GWAS SNPs which were also reported as liver eQTLs. This set of AS-SNPs represents candidate regulatory variants supported by LD association to liver-related diseases (GWAS SNPs) and with direct knowledge of the target of gene expression regulation (eQTL SNPs), hence designating ideal candidates for experimental validations.
Another two AS-SNPs associated to T2D were located on chromosome 17, where AS-SNPs rs28528789 and rs62075824 are in LD with the GWAS SNP rs12453394 in a similar fashion to the example reported above for chromosome 6 (Additional file 1: Figure S1). The two AS-SNPs are located in the first intron of the UBE2Z gene, which encodes for a ubiquitinating enzyme involved in signaling pathways and apoptosis. The ubiquitin-proteasome system has been suggested to play a role in the process of insulin resistance [7] and diabetes [8], making this locus of particular interest considering the close proximity of other ubiquitin-related genes like SNF8 and CALCOCO2. Another candidate target gene at this locus is GIP, which encodes for a potent stimulator of insulin secretion from pancreatic beta cells following food ingestion and nutrient absorption.
Genetic control of gene activity has been analyzed by the GTEx project in different tissues. The significant associations between SNPs and gene expression in the liver were collected from the GTEx project and intersected with the identified AS-SNPs. We found 292 unique AS-SNPs associated to gene expression (Additional file 9: Table S5). The genes with genetically controlled expression and associated to AS-SNPs were highly expressed in hepatocytes (ARCHS4 tissues, GTEx). We observed an enrichment in pathways involved in the regulation of immune response with several AS-SNPs associated to the expression of HLA genes.
Functional annotations of motifs
In order to obtain TFs active in the liver whose motifs were altered by the functional variants, we overlapped the collection of AS-SNP and TF motifs according to the funMotifs framework (see the "Methods" section). As a result, 595 AS-SNPs were annotated to the TF motifs in liver tissue. We obtained the functional score for each motif using the funMotifs framework. Using the functional score and other parameters introduced for the candidate functional motifs (Umer et al. "funMotifs: Tissue-specific transcription factor motifs", submitted), we identified 134 variants in 166 functional TF motifs (Additional file 10: Table S6). The majority of TF motifs were located in transcription start site (TSS) regions (Additional file 2: Figure S2). The most recurrent motifs altered by AS-SNPs were observed for the TFs: EGR1, CTCF, KLF5, and ZNF263 (Additional file 3: Figure S3).
The early growth responsive gene-1 (EGR1) is a zinc finger TF that plays an important role in metabolic processes [9] like regulation of cholesterol biosynthesis genes in the liver [10] or insulin resistance in type 2 diabetes [11]. Furthermore, downregulation of EGR1 has been associated to hepatocellular carcinoma (HCC) development [12]. Motifs for EGR3, belonging to the family of EGR1, were also altered by AS-SNPs but to a lower extent.
Krüppel-like factors (KLFs) are TFs that regulate several metabolic pathways, and deregulation of KLFs has been linked to metabolic abnormalities, such as obesity, diabetes, and heart failure [13]. KLF5 has been associated to the onset of fatty liver disease, promoting hepatic lipid accumulation [14]. The motif analysis is aimed at identifying which TF could mediate the effect of the candidate regulatory AS-SNPs potentially altering a downstream gene expression. We identified 13 AS-SNPs altering functional TF motifs which are also reported as liver eQTLs in the GTEx catalog (Additional file 10: Table S6). One example is rs4886705 that is a reported eQTL for the MAN2C1 gene (Additional file 4: Figure S4).
MAN2C1 encodes for an enzyme involved in the catabolism of cytosolic-free oligosaccharides, which are released from the degraded proteins. Overexpression of MAN2C1 has been linked with high mannose levels in the cytosol that could interfere with glucose metabolism [15]. The AS-SNP rs4886705 (A/G) alters a motif for HINFP, a zinc finger TF that interacts with the histone deacetylase complex and plays a role in transcription repression. It may potentially affect the repression of MAN2C1 leading to metabolic imbalance.
We identified 2329 heterozygous SNPs in a human liver sample that marked putative regulatory elements in the genome based on allele specificity measured through ChIP-seq experiments for histone modifications and TFs. Previous studies have indicated the extreme cell and tissue specificity of these regulators that can be active in one tissue and inactive in others, showing that experimental validations should be performed in the pertinent tissue [16]. At the same time, it is worth to note that gene regulatory mechanisms are often studied in cell lines derived from cancer cells [4]. These cell lines have single nucleotide and copy number variants that drive cancer and additional aberrations acquired during prolonged culturing, and all these variants could bias the interpretation of the molecular mechanisms [17]. Here, we report a collection of variants that flag candidate regulatory elements for liver- and metabolic-related diseases identified in the pertinent tissue context, a healthy human liver sample. Based on the Hardy-Weinberg equilibrium, 33% of common polymorphic sites are heterozygous in one person so in fact we interrogate a reasonable fraction of functional gene regulatory elements that are present in the liver.
The intersection of the liver-specific AS-SNP collection with GWAS and eQTL SNPs was aimed at adding a biological relevance layer. As observed before, the SNPs reported in GWAS and expression studies were directly supported by allele-specific signals in less than 10% of the cases on average. The vast majority of the identified AS-SNPs were in LD with reported GWAS or eQTL SNPs and likely to be the regulatory variants driving the associations. We identified 25 and 292 unique AS-SNPs associated to diseases of the liver and metabolism and gene expression respectively, providing new insights into the molecular regulatory mechanisms. AS-SNPs identified in the human liver flagged 17 genomic loci for several different liver-related traits and diseases.
An example is two loci on chromosome 6 and 17 associated to T2D where AS-SNPs can help explain the association observed in GWAS. The integration with GTEx expression data suggested that the regulatory mechanisms at these loci could link T2D to less familiar pathways such as cationic transporters and drug uptake on chromosome 6 and ubiquitination on chromosome 17.
We also intersected the collection of AS-SNPs with significant variant-gene associations from the GTEx project. We observed a significant number of candidate AS-SNPs (~ 17%) associated to the expression of HLA genes that are expressed not only in immune cells but also in most other tissues and cells. Several experimental and clinical studies have shown how inflammation and tumor progression are working synergistically [18]. An alteration of HLA gene expression can result in losing the ability to present antigens which have been reported to facilitate the metastatic process in cancer cells [19]. Moreover, HLA genes are overexpressed in hepatocytes of the liver with chronic damage or inflammation [20, 21].
The motif analysis was aimed at identifying possible mediators of the regulatory functional activity at the selected AS-SNPs. The rationale is that in altering the sequence of TF binding motifs the AS-SNPs could affect the expression of a target gene. We used functional motif definitions that overlay TFBS with several experimental datasets (e.g., ChIP-seq data, DHSs, CAGE peaks, and TF expression data) going beyond a simple coordinate overlapping with reported TFBS defined from PWMs. We intersected our liver-specific collection of AS-SNPs with functional motifs and found 134 AS-SNPs altering 166 defined functional motifs mostly for TFs expressed in the liver and associated to liver metabolic pathways and development of hepatocellular carcinoma (HCC), such as TFs belonging to the EGR and KLF families. The functional motifs were defined in HepG2 cells, an HCC-derived cell line. This could have led to a definition of more liver cancer-specific motifs and represents a limitation of the method. However, the definition of the functional motifs in the pertinent liver tissue context compensates for the lack of available genomic datasets for human liver tissue. The majority of altered functional motifs were located in the TSS, in agreement with the nature of the allele-specific signal defining the altering AS-SNPs, which in most cases was H3K4me3, a histone modification marking promoters. Finally, we identified 13 AS-SNPs that altered functional motifs and were also associated to gene expression in the liver in the GTEx project. This subset of AS-SNPs represents an excellent starting point for experimental validation of a possible molecular mechanism of gene regulation offering an educated guess on the target (eQTL) and the mediator (funMotifs) of the regulatory process.
In conclusion, we presented a systematic strategy to find functional gene regulatory variants, the TFs that bind differentially between alleles, and possible target genes in human liver tissue. The collection of AS-SNPs presented here offers a set of candidate regulatory variants supported by several layers of evidence to prioritize experimental validations aimed at improving the knowledge of the molecular mechanisms of many metabolic and liver diseases.
Liver WGS and creation of diploid genome reconstruction
Human liver tissue was obtained from Prof. Per Artursson, Uppsala University. The whole genome of the liver tissue was sequenced using the 10X Genomics technology that relies on linked-reads to provide long-range information usually missing from standard approaches, such as phasing and resolution of haplotypes and structural variants. Genomic DNA was extracted from the liver sample and sequenced to a 36x mean depth coverage. The Chromium™ Software Suite was used to analyze (Long Ranger) and visualize (Loupe) the linked-read sequencing data. We use the diploid genome reconstruction module from the ALEA toolbox [6] that takes a list of phased variants and a reference genome as the inputs. We utilized the variants called by Long Ranger with a "PASS" quality and the Genome Reference Consortium Human Genome Build 37 (GRCh37) as the backbone reference to build two in silico personal genomes for this specific liver sample. ChIP-seq reads aligning to the reference and alternative genomes are referred to as G1 and G2 in Fig. 1 and Additional file 7: Table S3, Additional file 8: Table S4, Additional file 9: Table S5.
ChIP-seq data
Aliquots of the tissue were grinded to a powder with liquid nitrogen, and ~ 40 mg or ~ 200 mg was utilized to prepare chromatin for histone modifications or TF ChIP using the Diagenode iDeal ChIP-seq kits for histones or TFs, respectively. We performed ChIP for two histone modifications: H3K27ac and H3K4me3, and a genome architectural protein: CTCF. Libraries were prepared from the enriched chromatin with NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, NEB) following instructions from the manufacturer and sequenced on HiSeq 2500 system with 100-bp pair end sequencing (Macrogen). The read quality was assessed using Phred64/33 scores with a quality cutoff requirement of 20.
AS-SNP definition
The AS-SNP discovery was adapted from our established modular pipeline [2] (available on http://bioinf.icm.uu.se/repositories.php, AS-SNP pipeline). In summary, (I) it realigns the ChIP-seq reads to two personal genomes derived from the reconstructed diploid genome, (II) it identifies heterozygous SNPs where the aligned read count differs statistically between the alleles, and (III) it filters out SNPs in blacklisted and duplicated genomic regions. All settings and controls are handled via a single configuration across modules.
Motif analysis
The potentially functional TF motifs were identified using the funMotifs framework that collected TF motif annotations across the non-coding regions of the human genome in a tissue-specific manner (Umer et al. "funMotifs: Tissue-specific transcription factor motifs", submitted http://bioinf.icm.uu.se:3838/funmotifs/). The AS-SNPs were overlaid onto the predefined TF motifs for each set of annotations received from a various data types: TF ChIP-seq data, DHSs, CAGE peaks, TF expression data, chromatin state, and information about replication domains. Annotations for the liver were obtained mainly from the HepG2 cell line data. For each of the TF motifs, a functionality score based on the weighted annotations was estimated. The TF motif was indicated as a candidate functional if the DNaseI signal was present on the motif, the TF was expressed, and the motif matching score changed at least 0.3. Furthermore, we required the TF binding event or the significant high functional score for the motif (no less than 2.55).
Genomic features
AS-SNP collections were intersected and filtered using several publicly available databases:
GWAS SNPs associated to selected liver- and metabolic-related traits from the NHGRI GWAS catalog [3]. A total of 5051 unique GWAS SNPs were retrieved in addition to 56,958 SNPs in high LD (r2 > 0.8) with them. A comprehensive list of the selected traits is reported in Additional file 5: Table S1
Collections of eQTL SNPs from the GTEx project. eGenes and significant variant-gene associations based on permutations in the liver (GTEx Analysis v7 eQTL) were obtained for a total of 290,178 significant associations
1000 Genomes SNP collection (1000 Genomes project, phase3-shapeit2-mvncall-integrated-v5a.20130502)
List of signal artifact blacklisted ENCODE regions [22], centromeric and telomeric regions
RegulomeDB [23]
ChromHMM [24] segmentations for the liver tissue from the Roadmap Epigenomics Projects (E066_25_imputed12marks_mnemonics.bed)
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The computations were performed on resources provided by the SNIC through Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX) under Project sens2017549. Prof. Per Artursson, Uppsala University, contributed the liver sample.
This work was supported by Astra Zeneca (C.W. and J.K.), the Swedish Diabetes Foundation (C.W.), EXODIAB (C.W.); the Family Ernfors Fund (C.W.); The Swedish Cancer Foundation (grant number 160518 to C.W.); The Borgströms-Hedströms foundation (M.C.); and The National Science Centre (grant number DEC-2015/16/W/NZ2/00314 to J.K.).
The authors declare that the data supporting the findings of this study are available within the article and its additional files. The raw ChIP-seq data produced are being submitted to the GEO database (ChIP-seq data) and the dbGaP (liver WGS data) and are currently available from the corresponding author on reasonable request.
Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden
Marco Cavalli
, Gang Pan
, José Ramón Bárcenas Walls
& Claes Wadelius
Department of Cell and Molecular Biology, Computational Biology and Bioinformatics, Uppsala University, Uppsala, Sweden
Nicholas Baltzer
, Karolina Smolinska Garbulowska
& Jan Komorowski
AstraZeneca, Gothenburg, Sweden
Chanchal Kumar
& Stanko Skrtic
Institute of Computer Science, Polish Academy of Sciences, Warsaw, Poland
Jan Komorowski
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MC and NB performed most of the data analysis. CW conceived and led the study. JRBW and MC performed the ChIP-seq experiments. JK and GP contributed to the data analysis and interpretation. CK and SS contributed to the results' interpretation. MC wrote the manuscript assisted by CW with input from all authors. All authors read and approved the final manuscript.
Correspondence to Claes Wadelius.
The study was approved by the Uppsala regional ethics committee (2014/433).
Figure S1. UCSC Genome browser view of the AS-SNPs rs28528789 and rs62075824 (yellow) which are in high LD with the T2D-associated GWAS SNP rs12453394 (cyan). TFBS from ChIP-seq data from the ENCODE project for HepG2 and other cell lines. (TIF 1913 kb)
Figure S2. Distribution of TFBS altered by AS-SNPs in different ChromHMM chromatin states. (TIF 260 kb)
Figure S3. Transcription factors whose binding motifs were most frequently altered by AS-SNPs. (TIF 202 kb)
Figure S4. UCSC Genome browser view of the AS-SNPs rs4886705 (yellow) which is a reported eQTL in the liver for the MAN2C1 gene (GTEx eQTL box plot). The histone modification tracks represent the peaks called for the ChIP-seq of H3K4me3 performed in the liver (black) and the HepG2 signal from the ENCODE project (pink trace) as a comparison. TFBS from ChIP-seq data from the ENCODE project for HepG2 and other cell lines. In the bottom panel, the motif for the HINFP transcription factor that is altered by rs4886705. (TIF 2872 kb)
Table S1. List of liver-related traits selected from the GWAS catalog. (XLSX 29 kb)
Table S2. Full collection of liver-specific AS-SNPs. (XLSX 187 kb)
Table S3. Collection of AS-SNPs associated to GWAS SNPs. (XLSX 16 kb)
Table S4. Collection of AS-SNPs associated to GWAS SNPs and reported liver eQTL in GTEx. (XLSX 12 kb)
Table S5. Collection of AS-SNPs reported as liver eQTL in GTEx. (XLSX 50 kb)
Additional file 10:
Table S6. Results of the funMotifs analysis. (XLSX 16 kb)
Cavalli, M., Baltzer, N., Pan, G. et al. Studies of liver tissue identify functional gene regulatory elements associated to gene expression, type 2 diabetes, and other metabolic diseases. Hum Genomics 13, 20 (2019) doi:10.1186/s40246-019-0204-8
Received: 15 January 2019
Regulatory SNPs


I would have been 20 weeks pregnant yesterday. Some people may not know how significant this week is, but moms do. I would be half way through the pregnancy. I would feel the movements of my little one from inside. We would know the gender and the name.
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Our society doesn't talk about it. We hear about abortion and Down Syndrome and heart defects and cancer and infertility. We hear about politics and mass-shootings and racism. So, why don't we hear about miscarriages? Maybe it is because moms don't talk about their baby in heaven. Maybe it is because we don't want to make people feel bad for us or make the situation awkward. Maybe it is because no one acknowledges our loss years or even months later. Maybe it is because we think others will believe that losing a baby at 6, 10 or 17 weeks pregnant isn't as big of a deal as losing a baby that actually lived outside of the womb. I'm not sure why our society doesn't talk about it, but I can tell you they should. Moms should talk about it. Doctors should talk about it. Teachers and politicians and celebrities and friends should talk about it. 1 in 4 women will miscarry. Think of four women. One of them has a hole in their heart that no one will ever be able to fill. Just because you don't hear about it, doesn't mean it's not happening.
To anyone who has lost their child through miscarriage, let me tell you, your loss matters. Whether you were 8 weeks or 17 weeks or 38 weeks. Your loss matters. So talk about it. I can promise you, I do. I tell everyone. I told the wedding venue and photographers. I told the salesperson at David's Bridal. I told people I don't even know. Why? Because moms talk about their children. It's what we do. It doesn't matter if we held them or not. I show everyone my angel's footprint, and I let them listen to the heartbeat. I don't want the memory of my child to disappear, so I'm not going to let it.
And to anyone who knows someone who has experienced miscarriage, whether it be last week, last month or last year, acknowledge their loss. Whether it is "are you okay?" or "you are so strong" or "I'm praying for you." It may be hard or awkward or scary for you, but it will warm their heart.
Moms of loss don't have pictures of their precious one giggling or videos of them crawling. We don't have measurements to share with you or a time of birth. We don't have a baby for you to hold and to kiss, but we have one waiting for us upstairs. Our angel matters to us, and it means the world to know they matter to you too.
"We wanted to write down exactly what it is like to lose a baby, but for some reason the page stayed empty.
Blessings on you. My heart aches for you and your extended family. Praying for come and for good to come from this tremendous loss. I think it already is.
I love you sweet pea. My heart aches for you and your family. My daughter also lost a baby and it's hard to know what to say at these times. How can you ever say anything comforting with such a GREAT loss. I am thankful that God has given you such an amazing support system in your family. And thank God for the hope that we will get to meet this little one in heaven. I love you and my continued prayers are always with you and your fiance.
This is so true and something I am working on trying to change through my blog. I want moms like us to be able to speak about their losses freely without guilt or shame. 20% of pregnancies don't end in a beautiful birth story. Women lose babies to miscarriage and stillbirth every day. It's time these babies acknowledged. Thanks for your post and my heart goes out to you.
Thank you so much!! I hope you have much success for your blog, and I am so sorry for your loss.


Spokusnyk' (Igor Khorosh, R. 2007). Seedling# IX-950405. TB, 39" (100 cm), Early midseason bloom. Standards and style arms pink violet (RHS 77B); falls same, lighter center; beards orange; lightly ruffled; slight sweet fragrance.


Farré Family, known as Family Canaro, began in hotel business in 1963 with the hotel La Posta Soldeu. It has 12 rooms.
In 1968 Mr. Josep Farré converted an old Borda (construction to keep save cattle in mountains) into a restaurant with 5 bedrooms. It was the first Parador Canaro. The word Parador is used to refer to a place to stop and to stay, in the way of merchants, farmers and other travelers.
Mr. Farré, a great entrepreneur, created in 1968 a ski area for beginners with a ski lift which was called Canaro, right next to the Hotel Parador Canaro.
In 1976 was installed the first snow canon around the Pyrenees.
In 1980 Parador Canaro renewed. 5 bedrooms extended to 18.
In 1981 owners carried out a new expansion. This time centered in ski slopes, new trail and ski lift was inaugurated, it was called Pedrico.
In 1991, 16 automated snow guns were installed around 2 ski slopes.
A few days later, in 2000, the ski area was extended to 3 hectares. Finally, in 2009, a complete renovation has done in the Hotel Parador Canaro reaching its current appearance. It has 64 comfortable rooms with magnificent views over slopes of Ski Parador Canaro and surrounding mountains.
Miquel Farré Casal grandfather, a famous cattle breeder in Andorra, was commissioned to carry letters every day to Porte, a French town. The French government awarded him for his work with the French medal of honor. As a gift gave him the first skis in Andorra. Today are exposed within the Parador Canaro as a tribute to this great man.
According to kilometres done carrying mail to France, by Miquel Farré grandfather during his career, would have been around the world for 10 times.


It was a game where the home side created plenty of chances, but it took the final 10 minutes to decide the course of the game.
Alnwick took the lead through Brad McClelland in the 15th minute, who finished well shooting across the goal and going in off the post for his 1st goal of the season.
Chances came and went for the home side, the closest being a Ross Straughan header from a corner kick that went just the wrong side of the post.
Esh Winning felt hard done to with a goal disallowed before half time due to a foul in the build-up.
The 2nd half was much of the same with Alnwick having the better of the chances, but they were made to pay when a well taken goal by Daniel Hill brough the away side level.
With the game seemingly slipping away, it was Brad McClelland again who converted from a tight angle at the back post to give Alnwick the lead again to make it 2-1 in the 80th minute.
The game was all but secured 2 minutes later, when substitute Kieran Hogg got on the end of a ball across the box, sliding in to lift the ball back across the goal into the top corner to give Alnwick their 3rd of the night.
In stoppage time, the hat-trick was complete for McClelland, after the referee waved play on after a number of attempts to bring players down, and head rounded the keeper to make it 4-1 and secure the 3 points.
"It's always nice to get the first win under our belts, but moreso was the manner of the performance and the self belief shown by the players to keep going and score the winning goals. Adam Laidlaw (1st team coach) has worked hard with the players on their passing and it's showing. It's also great to see over 100 fans turn out for us tonight."
First XI: Brooks C, Dundas L, Baker R, Brown T, Macpherson T, Straughan R, Jackson J, Egen K, McClelland B, Gair R, Riddell D.
Subs: Dixon J (on for Egen K), Glass C (on for Jackson J), Hogg K (on for Riddell D).


text:
G_BEGIN_DECLS

#define GTK_TYPE_CHROME_SHRINKABLE_HBOX                                 \
    (gtk_chrome_shrinkable_hbox_get_type())
#define GTK_CHROME_SHRINKABLE_HBOX(obj)                                 \
    (G_TYPE_CHECK_INSTANCE_CAST((obj), GTK_TYPE_CHROME_SHRINKABLE_HBOX, \
                                GtkChromeShrinkableHBox))
#define GTK_CHROME_SHRINKABLE_HBOX_CLASS(klass)                         \
    (G_TYPE_CHECK_CLASS_CAST((klass), GTK_TYPE_CHROME_SHRINKABLE_HBOX,  \
                             GtkChromeShrinkableHBoxClass))
#define GTK_IS_CHROME_SHRINKABLE_HBOX(obj)                              \
    (G_TYPE_CHECK_INSTANCE_TYPE((obj), GTK_TYPE_CHROME_SHRINKABLE_HBOX))
#define GTK_IS_CHROME_SHRINKABLE_HBOX_CLASS(klass)                      \
    (G_TYPE_CHECK_CLASS_TYPE((klass), GTK_TYPE_CHROME_SHRINKABLE_HBOX))
#define GTK_CHROME_SHRINKABLE_HBOX_GET_CLASS(obj)                       \
    (G_TYPE_INSTANCE_GET_CLASS((obj), GTK_TYPE_CHROME_SHRINKABLE_HBOX,  \
                               GtkChromeShrinkableHBoxClass))

typedef struct _GtkChromeShrinkableHBox GtkChromeShrinkableHBox;
typedef struct _GtkChromeShrinkableHBoxClass GtkChromeShrinkableHBoxClass;

struct _GtkChromeShrinkableHBox {
  // Parent class.
  GtkHBox hbox;

  gboolean hide_child_directly;

  // Private
  int children_width_requisition;
};

struct _GtkChromeShrinkableHBoxClass {
  GtkHBoxClass parent_class;
};

GType gtk_chrome_shrinkable_hbox_get_type() G_GNUC_CONST;

// Creates a new shrinkable hbox.
// If |hide_child_directly| is true then its child widgets will be hid directly
// if they are too wide to be fit into the hbox's width. Otherwise they will be
// shrunk first before being hid completely.
GtkWidget* gtk_chrome_shrinkable_hbox_new(gboolean hide_child_directly,
                                          gboolean homogeneous,
                                          gint spacing);

void gtk_chrome_shrinkable_hbox_set_hide_child_directly(
    GtkChromeShrinkableHBox* box, gboolean hide_child_directly);

gboolean gtk_chrome_shrinkable_hbox_get_hide_child_directly(
    GtkChromeShrinkableHBox* box);

void gtk_chrome_shrinkable_hbox_pack_start(GtkChromeShrinkableHBox* box,
                                           GtkWidget* child,
                                           guint padding);

void gtk_chrome_shrinkable_hbox_pack_end(GtkChromeShrinkableHBox* box,
                                         GtkWidget* child,
                                         guint padding);

gint gtk_chrome_shrinkable_hbox_get_visible_child_count(
    GtkChromeShrinkableHBox* box);

G_END_DECLS

#endif  // CHROME_BROWSER_UI_GTK_GTK_CHROME_SHRINKABLE_HBOX_H_

redpajama_set_name: RedPajamaGithub


There were some sandwich terns flying around the beach this afternoon. I didn't see the yellow tip to the black bills or the black crests as they were always in flight so I will refrain from saying they were gull-billed terns though I thought the wings had dark trailing edges but the bills were long and thin like a sandwich tern.
I am confident that what you saw were Sandwich Terns. Gull-billed is rare in the UK occurring mainly in the southern counties of England. I am not aware of any records for Sutherland or Caithness.
In flight Gull-billed has a grey rump and tail whereas in Sandwich they are white (although there are rare birds that have a pale grey rump but never as dark as Gull-billed). The bill of Gull-billed (as the name suggests) is stout and heavy with a pronounced gonydeal angle, the bill of Sandwich is long and slender with no marked gonydeal angle. The yellow tip of Sandwich is not always noticeable in flight, neither is the crest.
Gull-billed looks more chunky than Sandwich and its flight action is more gull like and not as bouyant as Sandwich.
Field guides usually show Sandwich with darker outer primaries contrasting with paler grey inner primaries. However, this pattern becomes more pronounced as the summer progresses and the feathers become warn. At this time of the year, soon after the partial moult into summer plumage, the contrast is less marked.
I hope that is of help.


Hamilton Medical on Twitter: "The HAMILTON-T1 transport ventilator ... Hamilton Medical on Twitter: "The HAMILTON-T1 transport ventilator for armed forces in the air, on land, and at sea. https://t.co/QVUWV7REo5… "
Hamilton T1 Ventilator User Manual - Home 製品から探す 特殊金属加工(Ti、Co-Ni). 特殊金属加工(Ti、Co-Ni) チタン・ コバルトニッケル (加工技術).


AMD is set to introduce a new mid-range graphics card based on the 40nm 'Barts LE' core before the end of the week, according to a report on NordicHardware. The Radeon HD 6790 would directly compete with Nvidia's GeForce GTX 550 Ti, offering a more powerful alternative to Radeon HD 5770 at a lower price than Radeon HD 6850.
Spec-wise the Barts LE core is said to feature AMD's VLIW5 architecture and will get 800 stream processors arranged in 10 streaming multiprocessor units -- which compares to 12 SM units and 960 stream processors in the Barts Pro GPU that powers the Radeon HD 6850. The card also sports 40 Texture Units, a core clock of 840 MHz, a 256-bit memory interface, 1GB of GDDR5 VRAM set to 4200 MHz, two 6-pin PCIe power connectors, and a TDP of 150W -- which oddly enough is higher than the more powerful Radeon HD 6850's rating of 129W and close to the 6870's 151W.
AMD will reportedly launch the new card on March 31. Although there's still no word on pricing, if it is to compete with the Nvidia GeForce GTX 550 Ti we can expect AMD's latest mid-range offering to hover around the $150 mark.


MinedBlock is an overseen crypto mining administration. We will circulate an offer of mined income to our token holders every month. Basically hold the MBTX token in your ETH wallet to develop your portfolio.
Pallium is a stage for the fast adapting, simple sending, and secure stockpiling of AI models in a decentralized situation.
Crowd Machine is powering the next generation of decentralized blockchain applications. Our mission is to disrupt multi-trillion dollar industries by providing a low cost compute infrastructure platform for the execution of decentralized apps and allowing anyone to generate wealth through the technology decentralization revolution.
Telescopia offers uncommon multilingual live telescope encounter that embodies a tremendous system of administrations offered in one package containing: live control, video feed, specialists, visit guides, proficient picture catching (astrophotography), infra-red space science, address rooms, blended reality encounter all through all administrations and a telescope commercial center.
FoodNation is a sustenance conveyance commercial center, grew cooperatively, utilizing the blockchain innovation and having as key part the circulation of the benefits with the merchants and divulgers for a more popular and supportable developing.
NWP is a stage that utilizations smart gadgets and information examination to assemble, total and dissect significant social insurance learning, offering some incentive to human services and personal LIFE SERVICE.
WokeCoin is a token based on a fictional technology that merges mindfulness and tangible value. All ETH raised during the ICO will go to MAPS Canada for psychedelic research.


text:
Q: Kendo grid sorting doesnt work after grouping I have a Kendo UI grid. I want to display data as grouped and sorted, but seems that doesnt work. In my dataSource i have
                   serverPaging: true,
                   serverSorting: true,
                   serverFiltering: true,
                   group: {
                        field: "ApplicationName",
                        dir: "desc"
                   },
                   sort: {
                        field: "VersionTo",
                        dir: "desc"
                   },

Grouping works...But data is not sorted.
If i remove group property then i get data sorted but not grouped.
From a google search i see that serverGrouping is not enabled, so grouping happen on client-side and change sorting. But if i add in my configuration "serverGrouping: true," i get this error "Uncaught TypeError: Cannot read property 'length' of undefined".
How can integrate grouping and sorting?

redpajama_set_name: RedPajamaStackExchange


text:
Amazon Prime Video Stories September 4, 2018
Netflix and Amazon Prime Video will have to make more European content – by law
Ben Lovejoy - Sep. 4th 2018 3:56 am PT
Streaming video services like Netflix and Amazon Prime Video will soon have to ensure that at least 30% of the programming offered in Europe is produced in EU countries …
Amazon Prime Video Stories December 18, 2017
Amazon Prime Video app reportedly set all-time tvOS record for early downloads
- Dec. 18th 2017 7:47 am PT
Apple TV Amazon Prime Video
If ever a tvOS app was likely to prove a hit, it was Amazon Prime Video. Apple TV owners have had a very long time to wait for it to arrive on the platform, Apple promised it in more than one event, and when it finally did land, Apple heavily promoted it.
So it would be no great surprise if one claim being made for the app were to prove correct …
expand full story
Amazon Prime Video Stories December 5, 2017
Apple mistakenly starts promoting Amazon Prime Video for Apple TV ahead of release [U]
Chance Miller - Dec. 5th 2017 6:12 pm PT
Update: Now available!
As we approach the new year, Apple has two promises left to fulfill: the iMac Pro and Amazon Prime Video for Apple TV. While all has gone quiet about the former, it appears that we're finally nearing a launch for Amazon Prime Video for Apple TV…
[Test] Image Aspect Ratio
[Test] Characters in titles ' ! @ # $ % ^ & * ( ) ' ' ? < > : ; | \ { } [ ] – + =
[Test] Tables
Formula E races on full historic Monaco circuit for the first time this weekend
Amazon Prime Video Stories June 1, 2016
Amazon CEO Jeff Bezos explains why the company no longer sells the Apple TV
- Jun. 1st 2016 4:44 am PT
Apple TV Amazon Amazon Prime Amazon Instant Video Amazon Prime Video
If you search Amazon for streaming media players, you'll find plenty of them. The one thing you won't find is the Apple TV, and company CEO Jeff Bezos told the Re/code conference why not. Apple TV doesn't currently offer access to Amazon's own Prime Video platform, and Bezos says that isn't because Apple refuses to include it, but because it wants too much money for doing so.
[W]hen we sell those devices, we want our player — our Prime Video player — to be on the device, and we want it to be on the device with acceptable business terms. You can always get the player on the device. The question is, can you get it on there with acceptable business terms?
While he doesn't say so outright, the meaning of the vague phrase is pretty clear …

redpajama_set_name: RedPajamaCommonCrawl


British luxury fashion house Burberry intentionally destroyed more than $36 million worth of its own products in the past year alone, the Guardian reports. Let me be clear—the label literally burned fashion and cosmetic products carrying the Burberry trademark, because capitalism is a nonsensical loop of wastefulness.
Burberry explained that the cosmetics needed to be destroyed because its beauty line has recently been acquired by Coty, a multinational beauty company. However shareholders at the company's annual meeting demanded to know why investors weren't given the chance to purchase some of the unsold stock.
I would personally love to know why the clothing wasn't recycled, or donated, or sold at a discount to raise money for charity, or used for literally anything else instead of being sent up in ozone-depleting air-polluting capitalism-induced flames. Stitch all those coats and scarves together to make a giant Burberry-branded hot air balloon that can carry us up and away from this hellish planet.
Burberry claimed to take environmental concerns of this destruction very seriously, and used special incinerators that allow the energy from the burning process to be harnessed for other uses. This is my official request that we use that energy to power a giant blimp that reads "End Sweatshop Labor." Or, you know, pay workers in the garment industry a fair living wage.


Sept. 2006 We burn our mortgage on the new Church!
Dec. 2003 First service held in our new sanctuary.
Dec. 2001 New Educational/Administrative wing dedicated.
Oct. 2000 Groundbreaking on our current Church.
Mar. 1997 Building program initiated with God's will.
Sept. 1996 Vicar Owen Griffiths installed for one year term.
Sept. 1995 Vicar Pam Wellons installed for one year term.
May 1983 Dedication of our first Sanctuary and Fellowship Hall – now replaced by the current Church.
Dec. 1972 Pastor Ronald Gadde begins his ministry at Grace.
July 1969 Pastor Jeffrey Burnoski begins his ministry at Grace.
Sept. 1967 Groundbreaking on first Sanctuary.
Sept. 1964 First Grace worship service held in a store front.
Feb. 1964 Pastor Albert Haversat begins a mission to start a Lutheran Congregation in Jefferson Valley.


text:
Emails by Sector
Emails by Region
HomeFeatured ItemsAll Sectors
The Schools' Email Database includes contact details for all schools in England. The database is current, and was last updated in February 2017 with the very latest information. The database has 26 fields which offer useful information on the age ranges of the children, phone and fax numbers, the headteacher's name, the school address, postcode, establishment name, website and email address.
The database covers all phases of education: from nursery and primary schools, through to middle schools, secondary schools and 16+. The database also provides information on the types of establishments. In England, these include community schools, nursery schools, foundation schools, voluntary controlled schools, voluntary aided schools, independent schools, sixth form centres, university technical colleges, special schools and pupil referral units.
Total number of records:24876
Records with websites:21167
Records with email address:23698
Records with Phone Number:24774
Number of fields per record:26
All Sectors quantity
Price:£39
Price:£9
Pupil Referral Units
SchoolEmails.co.uk are one of the leading suppliers of school email addresses in the UK.


text:
Q: Nested object array Javascript regarding oops return method You are given an object which has details of a particular class. Complete the function so that it returns the details of the student at i th index in the form of an array.
Input Format: Given the index (i) of the student whose details should be printed.
Output Format: An array containing name and roll no of the student.
Sample Input:
1

Sample Output
Bhavya
2

My output is somewhat wierd, can anyone help me out? how to resolve this?
 function studentDetails(i) {
        var classDetails = {
            classTeacher: 'Anjani',
            students: [{name: "Anjali", roll: "1"}, {name: "Bhavya", roll: "2"}, {name: "Neha", roll: "3"}]
        };

       return classDetails.students[i].name;

      }

    process.stdin.resume();
    process.stdin.setEncoding("utf-8");

    let remainder = '';
    process.stdin.on('data', function (chunk) {
        let t = parseInt(chunk);
        let ans = studentDetails(t);
        for(var i=0; i<ans.length; i++)
        {
            process.stdout.write(ans[i]+'\n');
        }
        process.exit();
    });

A: var arr = []
arr.push(classDetails.students[i].name);
arr.push(classDetails.students[i].roll);
return arr;

A: studentDetails() needs to return the entire object, not just the name.
Then there's no need for a loop, just print its name and roll.

function studentDetails(i) {
  var classDetails = {
    classTeacher: 'Anjani',
    students: [{
      name: "Anjali",
      roll: "1"
    }, {
      name: "Bhavya",
      roll: "2"
    }, {
      name: "Neha",
      roll: "3"
    }]
  };

  return classDetails.students[i];

}

process.stdin.resume();
process.stdin.setEncoding("utf-8");

process.stdin.on('data', function(chunk) {
  let t = parseInt(chunk);
  let ans = studentDetails(t);
  process.stdout.write(ans.name + '\n');
  process.stdout.write(ans.roll + '\n');
  process.exit();
});


text:
Contact PACER | Search
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PACER Puppets
PACER Puppets Overview
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COUNT ME IN®
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Learn more about the PACER Puppets in these videos.
Puppet Interviews
Ben answers questions about what it's like to have ADHD. He discusses ways he has learned to get along better in school and at home. (2:07)
Gina answers questions about what it's like being blind. She explains how she gets dressed and matches her outfits, how she can participate in activities like swimming and beep ball, and the future of self-driving cars. (1:39)
Mitch has recently learned about autism. He answers questions about Megan, a girl in his class, who has autism, like why she eats the same thing for lunch every day, why she doesn't make eye contact and how he's learning how to understand her better. (1:43)
Mitch answers questions about how autism manifests differently in different people and specific questions about Max, a kid in his class, who has autism. Mitch also explains how he and Max became friends. (2:11)
Sally answers questions about cerebral palsy and what it's like to do activities like getting dressed if her legs and muscles don't move very well. (1:07)
Sally answers questions about being in a wheelchair, like how she goes upstairs and gets on and off the school bus. (1:10)
Sally talks about activities she likes to do. She discusses learning how to play basketball in a wheelchair basketball league, how she uses ball ramps when she goes bowling, and how she gets into the pool and swims. (1:05)
Corey answers questions about baseball, movies, hamburgers, and what it's like living with Down syndrome. (1:37)
Kindness and Inclusion
Shannon surprises Carmen by writing a kind message in chalk in front of her house to make amends for her previous bad behavior. (0:53)
Brad reaches out to his friend Mitch over video call when he is being picked on by his older brother. (1:25)
Brought to you by PACER's National Bullying Prevention Center, Carmen's Corner is a series of educational videos designed for young audiences kindergarten through third grade. Carmen focuses on kindness and offers tips for kids on how to make the world a kinder place. (12:09)
This episode of Carmen's Corner focuses on "feelings." Carmen and friends practice identifying and naming emotions and discuss different coping skills.. (15:29)
This episode of Carmen's Corner focuses on "acceptance." Carmen and friends celebrate their differences and similarities and learn that it's okay to be different. (7:47)
This is part 2 of episode 3 of Carmen's Corne, which focuses on "acceptance." Carmen and friends celebrate their differences and similarities and learn that it's okay to be different. (7:07)
Our Puppet Maker
Charlotte has been making puppets for PACER since 1995. We interview her in her basement workshop. PACER puppets are available for purchase.
(8:00 a.m. - 5:00 p.m. CST, M-F)
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PACER is the Minnesota Parent Training and Information Center, funded by the U.S. Department of Education's Office of Special Education Programs
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Selective iron chelation in Friedreich ataxia: biologic and clinical implications.
Iron and Pseudomonas aeruginosa biofilm formation.
Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity.
TonB-dependent iron acquisition: mechanisms of siderophore-mediated active transport.
Quercetin prevents DNA single strand breakage and cytotoxicity caused by tert-butylhydroperoxide: free radical scavenging versus iron chelating mechanism.
Protection against oxidative damage of erythrocyte membrane by the flavonoid quercetin and its relation to iron chelating activity.
Iron-catalyzed hydroxyl radical formation. Stringent requirement for free iron coordination site.
Non-specific serum iron in thalassaemia: an abnormal serum iron fraction of potential toxicity.


The Azure Big Compute team is happy to announce version 1.0.0 of the Batch Shipyard toolkit, which enables easy deployment of batch-style Dockerized workloads to Azure Batch compute pools. Azure Batch enables you to run parallel jobs in the cloud without having to manage the infrastructure. It's ideal for parametric sweeps, Deep Learning training with NVIDIA GPUs, and simulations using MPI and InfiniBand.
Whether you need to run your containerized jobs on a single machine or hundreds or even thousands of machines, Batch Shipyard blends features of Azure Batch -- handling complexities of large scale VM deployment and management, high throughput, highly available job scheduling, and auto-scaling to pay only for what you use -- with the power of Docker containers for application packaging. Batch Shipyard allows you to harness the deployment consistency and isolation for your batch-style and HPC containerized workloads, and run them at any scale without the need to develop directly to the Azure Batch SDK.
We hope to continue to expand the repertoire of recipes available for Batch Shipyard in the future.
The Batch Shipyard toolkit can be found on GitHub. We welcome any feedback and contributions!


The Web has changed: services become resource-oriented APIs. We must react now.
Exploring the opportunities resource-oriented APIs offer, especially in combination with links.
Motivated researchers from the REST, Semantic Web, and Linked Data communities.
A truly interactive workshop, driven by constructive discussion and dialog.
An inspiring day. Morning: talks and dialog. Afternoon: brainstorm and discussion.
Interested? Take a look at the Call for Papers or the workshop program.
Services and the Semantic Web: it's complicated.
The Semantic Web's relationship with services has been a long and complicated one. Many of the early service description efforts had a solid theoretical foundation, yet failed to find broad adoption.
The Web changed a lot since then, and so did the mindset of its researchers.
Today, resource-oriented APIs or "REST APIs" are on the rise – and herein truly lies an important opportunity for the Semantic Web. Services no longer have to exist on a separate island, because the Semantic Web shares with these REST APIs the most essential feature of the Web: resources.
What is our answer, as a community, to this evolution?
With this workshop, we aim to discover new ways to embrace the opportunities that resource-oriented APIs offer to the Semantic Web. We want to challenge researchers to think out of the current box through both paper submissions and interactive on-site discussion and dialog.
Can links do for APIs what they did for the Semantic Web?
The Linked Data movement has provided the Semantic Web with a powerful injection thanks to the power of links. Since REST APIs also center around resources, key questions for the workshop are: How can we enable Linked APIs? And how can they create added value for the Semantic Web?
We look forward to submissions that tackle these and related important questions, by focusing on the specific nature of resource-oriented APIs, the functionality they bring, and their path to integration.
We also actively invite participants to discuss and challenge new ideas.
The notion of Web services has been around since the beginning of the Web. When the Semantic Web community started evolving, it thus came as no surprise that people had the desire to work on the bridge between services and semantics, especially given the importance of services in various agent scenarios for the Semantic Web. Since SOAP and WSDL were the predominant service technologies at that time, researchers worked on describing their low-level technical details – such as parameters and exception handling – in semantic models, notably OWL-S and WSMO.
When SOAP and WSDL turned out not to be a satisfying answer for a resource-oriented Web, providers shifted from Web services to resource-oriented APIs, commonly called REST or RESTful. In response, researchers ported the concept of describing low-level details to this new situation, giving rise to WADL, MicroWSMO, and others.
However, the ideas themselves were not questioned: can technical descriptions alone satisfy the vision and aspirations of the Semantic Web? New paradigms do not only demand new answers, but – more importantly – new questions.
The questions we look for can be found in different sources.
One of them is the initial Semantic Web vision: what are the missing links to construct a Web for agents, given the Linked Data cloud and resource-oriented APIs? Another source stems from concrete business situations and needs: what do current resource-oriented APIs lack on a semantic level, and how can we provide this?
Clearly, these questions convey fundamental problems that have been neglected for too long.
We're sure that several points in our Call for Papers will appeal to you.
Help us reach a larger audience during the workshop!
Spread the word on Twitter and follow us – we'll follow you as well.
Read more about the ESWC 2012 conference and find out about registration, the venue, and travel arrangements.


Super soft and casually sporty – this soft knitted jumper creates clear, fashionable accents with its large hood and long drawstring ties.
I had bought the same model in light grey. I like the shape, the lenght, the hoodie. Good texture and great colour.
Lovely soft material. Cosy and warm. Beautiful colour, going to love wearing this over Christmas!


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From the Lesson Tee to the Course: A Naturalistic Investigation of Attentional Focus in Elite Golf
Steven Orr, Andrew Cruickshank, and Howie J. Carson
While debate continues on "optimal" attentional focus, little empirical knowledge exists on the way that attention is operationalized across training and performance in elite golf. Accordingly, this study aimed to (a) explore the attentional foci promoted or used by coaches and players for different types of shots in training, plus their underpinning rationale and (b) explore the attentional foci promoted or used by coaches and players in competition, plus their underpinning rationale. Our findings revealed that (a) various foci were used across training and competition; (b) all players used different combinations of foci across training and competition, and within different aspects of training itself (e.g., short vs. long game); and (c) players often used alternative or additional foci in training to those promoted by coaches, and self-generated foci for competition. These results highlight the complexity and practical reality that needs to underpin future advances in theory, research, and practice.
In The Sport Psychologist Volume 35 (2021): Issue 4 (Dec 2021)
Journey From Control to Liberation: Exploring Student-Athletes' Physical Activity Perceptions and Experiences in the Transition Out of Collegiate Sport
Erin J. Reifsteck, Jamian D. Newton, Melinda B. Smith, DeAnne Davis Brooks, and Shelby N. Anderson
There is growing interest in how athletes' physical activity participation may be impacted when they transition out of competitive sport; however, few studies have examined the process of physical activity transitions in collegiate student-athletes using a qualitative approach. The purpose of our study was to explore student-athletes' perceptions of, and experiences with, physical activity in the transition out of collegiate sport. Our analysis of transcripts from 13 focus groups conducted with current and former student-athletes (n = 59) suggests that student-athletes experienced a journey from control to liberation as they transitioned into their postcompetitive lives. In this exciting yet challenging transitional journey, participants were faced with navigating newfound autonomy over their physical activity outside of the controlled environment of collegiate sports and were considering the value and meaning of physical activity within a health promoting context. We offer practical recommendations from these findings to support student-athletes in this transition.
In The Sport Psychologist Volume 36 (2022): Issue 1 (Mar 2022)
"Short and Sweet": A Randomized Controlled Initial Investigation of Brief Online Psychological Interventions With Endurance Athletes
Carla Meijen, Alister McCormick, Paul A. Anstiss, and Samuele M. Marcora
There is potential in delivering brief, educational interventions online, particularly for recreational athletes. This initial investigation examined how two online interventions were perceived by endurance participants and how they affected outcomes of interest. After measuring self-efficacy, 142 people were randomized to one of three groups (self-talk, implementation intentions, and control) before an endurance event. Ninety-four completed postevent measures, which were self-efficacy, goal attainment, performance satisfaction, coping, stress appraisals, and social validity. The interventions involved approximately 10 min of initial engagement with online material. Perceptions of stress controllability were significantly higher in the implementation intention group compared with the control. There were no other statistically significant effects. Nevertheless, both intervention groups were satisfied with their interventions, found them useful, and were planning to continue using them. The findings demonstrate the feasibility and value of using brief, online psychological interventions, which may be timely in our changing profession, as COVID-19 has moved many interventions online.
Stress-Related Growth in Elite Sport Performers: Qualitative Differentiators in Psychosocial Mechanisms
Fionnuala B. Barnes, David Fletcher, and Kacey C. Neely
The purpose of this study was to explore growth following the experience of stressors and compare the experiences of elite athletes who exhibit higher and lower levels of growth. Six elite athletes (five female and one male) participated in a semi-structured interview. Three athletes reported experiencing higher levels, and three athletes reported experiencing lower levels of growth. Interpretative phenomenological analysis revealed that understanding of self, development in athletic identity, and social support are key psychological mechanisms, which differentiate elite athletes who reported experiencing higher and lower levels of growth. Athletes higher in reported growth showed greater association with meaningful behavioral actions, ultimately reflecting the modification of previously held beliefs into a new worldview. Athletes lower in reported growth reflected an attempt to maintain beliefs into an already existing worldview, thus hindering growth. The findings show psychological mechanisms that accumulatively promote growth and provide a foundation for subsequent intervention studies.
In Pursuit of Excellence: How to Win in Sport and Life Through Mental Training (5th Ed.)
Jen Schumacher
Coaches' Perceptions of Mental Toughness in Adolescent Athletes: A Phenomenological Exploration
Johannes Raabe, E. Earlynn Lauer, and Matthew P. Bejar
Mental toughness (MT) enables individuals to thrive in demanding situations; however, current conceptualizations of MT are primarily based on research with elite adult athletes. Thus, the purpose of the current study was to explore youth sport coaches' perceptions of mentally tough adolescent athletes with whom they have worked. Phenomenological interviews were conducted with 14 youth sport coaches (nine men and five women). Using a hermeneutic process, a thematic structure comprising five themes was developed: (a) Youth athletes demonstrate their MT by overcoming various obstacles, (b) mentally tough youth athletes are highly self-determined with respect to their sport participation, (c) mentally tough youth athletes control their emotions in competition, (d) mentally tough youth athletes focus on aspects that facilitate their performance, and (e) mentally tough youth athletes are good teammates. These findings not only complement existing conceptualizations of MT but also highlight important distinctions in the manifestation of the construct in early to middle adolescents.
Organizational Systems in British Sport and Their Impact on Athlete Development and Mental Health
Zoë A. Poucher, Katherine A. Tamminen, and Christopher R.D. Wagstaff
Sport organizations have been noted as pivotal to the success or failure of athletes, and sport environments can impact the well-being and development of athletes. In this study, the authors explored stakeholders' perceptions of how high-performance sport organizations support athlete development. Semistructured interviews were conducted with 18 stakeholders from the United Kingdom's high-performance sport system and transcripts were analyzed using a semantic thematic analysis. Participants emphasized the importance of performance lifestyle advisors, sport psychologists, and financial assistance for promoting athlete development. Several stakeholders observed that despite the extensive support available to athletes, many do not engage with available support, and the prevalence of a performance narrative has led to an environment that discourages holistic development. It follows that sport organizations could develop alternative strategies for promoting athletes' access to and engagement with available supports, while funding agencies might broaden existing funding criteria to include well-being or athlete development targets.
The Effect of Transformational Leadership and Well-Being on Performance of Soccer Players: An Inclusive Model
Sinan Yildirim and Ziya Koruç
The current study focuses on the effect of transformational leadership on athletes' performance in the mediation of psychological need satisfaction, burnout, competition anxiety, life satisfaction, and positive–negative affect. The sample consisted of 391 soccer players aged between 16 and 20 years. Six scales were used in this study: Multifactor Leadership Questionnaire, Needs Satisfaction Scale, Athlete Burnout Measure, Satisfaction with Life Scale, Positive and Negative Affect Scale, and Sport Competition Anxiety Test. The method of Vallerand was preferred to measure performance, and structural equation modeling was employed to analyze data. The model data fit was also verified. It was found that the transformational leadership behaviors of coaches signally influence athletes' performance either directly or indirectly. From another perspective, increasing the psychological health or well-being of athletes has important effects on sport performance.
Volume 35 (2021): Issue 3 (Sep 2021)
A Survey of Applied Impact of Literature in Sport Psychology
Andrew P. Friesen
There has been an implied direct connection between the scholarly literature and applied practice. However, the sport and exercise psychology community is lacking an empirical account of what practitioners believe to have been the most impactful scholarly writings to their applied practice. The purpose of this study was to survey applied practitioners of their perceived most impactful scholarly writings to their professional practice. Surveys were returned from 532 participants solicited from the Association for Applied Sport Psychology membership, who were asked to identify their perceived most impactful book and journal article to their practice. Frequency statistics were calculated and presented for topic, type, title, author(s), year published, and journal. A total of 143 different books and 188 different articles across 84 different journals were reported. Implications for applied practice, teaching sport and exercise psychology, and research are presented.
In The Sport Psychologist Volume 35 (2021): Issue 3 (Sep 2021)


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Your Bed In Tokyo

by Jim Walls on March 19, 2016

I didn't tell you that it had nothing to do with you although I did do it because I knew I could stay in your bed in Tokyo. I didn't tell you that it took 8 days for my family to figure out I wasn't in America anymore.

You didn't know all the reasons why I flew across the ocean so far that it became a different day. You didn't know why I got fired from my job or why I used all my savings to buy a round trip ticket and that I didn't plan on going home. You didn't know that I wasn't on vacation.

We spent a lot of time watching movies on your laptop on your bed with your cat. Most of the time we were talking more than watching. I suggested that we buy tickets to this place I had read about on the internet called the Suicide Forest. I said:

"Not to do it together. Just to see it."

So we bought tickets for at the end of September. At the beginning of September I ran out of money but I cancelled my return flight anyway. And on the night my flight left without me I got into bed naked with you and you took off your clothes too. The day after that we started fighting. You later admitted you felt hurt about reproached advances when you told me:

"You think you're above the law of the way things work."

You resented funding what you saw as my vacation but you continued to pay for my food and alcohol and cigarettes and share half of your bed with me even though we never fucked again after the first time. But we still slept naked and I still watched you get dressed every morning. I later claimed I was only drunk when I yelled:

"Everyone hates me because I can't love them."

And it started to become clear that we wouldn't make it to the Suicide Forest together. I started going out at night and waking up on the sidewalk. You started planning a backpacking trip through southeast Asia. The idea of you traveling for a month alone through third world countries seemed unlikely to me. I said:

"You won't be able to talk to anyone for a month."

And then I realized I wouldn't either. You said:

"I've traveled abroad before. I think I got it."

I hated that you used the word abroad. I said:

"I guess only rich people get poor countries."

I didn't believe you would actually go until I found an envelope full of yen on your bed and a note that said:

-Please don't let my cat die.

When I dropped the note was when I first thought about what death might be like. It could be a screen that displayed "oops over" for 5 seconds in size 12 font and then went blank forever. I imagined your body covered in rat-sized bugs as it floated down a river in the Cambodian jungle and then:

-Oops over.

And when I saw people in the street who I could never talk to because they didn't speak my language and I didn't speak theirs I thought to myself:

-Oops over.

I wished I had the power to see inside them instead of the power to imagine them dead. And then I thought:

-There's too many people in this world.

That was the thought that made me drop the cigarette that burned the little crater in your cat's ear. Her eyes reflected back at me with a yowl when I tried to reach under the bed to pet her. She couldn't understand the concepts of accident, apology, regret. So I let her outside and she never came back.

When my sister found out I was in Japan she had sent me an email. Part of it said:

-Cutting out the things that you think are making you unhappy won't make you happier but I guess you're getting closer to the problem.

I read this again while on the bus to the Suicide Forest. The weather was gray and on the horizon I saw 2 dark slopes rising to meet each other before getting lost in the clouds. I thought it might be the base of Mt. Fuji but I had never seen a mountain before so I couldn't believe that it could be that big and I tried to email my sister:

-Tell dad I'm ok.

But my phone died and behind my eyelids was my name displayed in size 6 font.

Inside the Suicide Forest was all green moss coating everything and when I went in far enough I could barely hear anything at all. I forgot to buy a ticket back to Tokyo and I didn't even know where I was. I was inside the Suicide Forest near the base of maybe Mt. Fuji. Even when it started getting dark it seemed unlikely to me that I could get myself killed while traveling in the first world.

But inside my head were 3 mental images:

-The envelope full of yen on your bed in Tokyo.

-My dad's blue truck in Delaware.

-You pulling on your briefs while holding a lit cigarette.

And these things felt like they were dissolving into a blank screen. By the time the sun rose I was still in the Suicide Forest near the base of what was probably Mt. Fuji but I felt like I was barely there at all.

    Your Bed In Tokyo - March 19, 2016 -

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Here's some bad news for the nascent Sony Marvel Universe: one of its upcoming superhero entries, Silver and Black, no longer has a release date. Meanwhile, A24's Under the Silver Lake, an indie film starring Andrew Garfield, has been delayed until December. Read more about both below.
We've always thought Sony's idea of creating a universe of Spider-man-centric Marvel comic book characters that's entirely separate from Marvel Studios' Marvel Cinematic Universe was weird. While it appears that Venom (the first movie in the Sony Marvel Universe, or "SMU") will make it to theaters on schedule, the next planned entry in that universe has hit a major speed bump.
Sony's SILVER AND BLACK was set for Feb. 8, 2019, but is now listed as the worst date possible…UNDATED.
Silver and Black is a female-led superhero movie that centers on Silver Sable and Black Cat, two supporting characters in Spider-Man comics. Silver Sable is a mercenary who hunts war criminals, while Black Cat, AKA Felicia Hardy, is a master thief. In the comics (and the 1990s animated TV series), Felicia Hardy is a love interest for Peter Parker.
Gina Prince-Bythewood, the director of movies like The Secret Life of Bees, Love and Basketball, and Beyond the Lights, is in the director's chair for this one. (She also directed the pilot of Marvel's Cloak & Dagger TV series for Freeform.) I was looking forward to seeing what she could do with this, but now we may be in for a long wait.
We were just weeks from seeing this, but now the movie has been bumped all the way to December 7, 2018.
A24's UNDER THE SILVER LAKE will go into hiding for 6 months and reappear Dec. 7, 2018. Previous date was just 3 weeks away–June 22.
Some film reporters who have seen the movie at film festivals already are suggesting that this delay could imply that A24 is re-editing the movie. Many reviews have said that the film is messy, and the overarching implication is that there's a good movie buried somewhere in its two-hour-and-twenty-minute runtime.
This could easily be explained by tracking on Hereditary and not wanting another film out there to promote two weeks later. And this way it can play fall fests. Makes sense.
A24 has a stone-cold classic on their hands with Hereditary (read our review here), so pushing this in order to devote all of their time and attention to Hereditary getting its proper due may be a smart move for both films. Plus, if they're confident in Under the Silver Lake's trippy noir vision, they may be positioning it for awards contention by slotting it later in the year. Either way, I'm looking forward to checking it out as soon as possible, because I'm super curious about this one.


text:
April 21, 2016 Souls
I had been wanting to capture Adam for years. Adam heads up our local division of Sea Shepherd. The stories of the seas etched in his face, his furrow and lines show a life of adventure and exploration. He reminds me of a 'captain of the sea', self assured, passionate and extremely outspoken. When Adam started with Sea Shepard he tells us, he was very shy. I smile as this seems so far from the man who stands before me. I tell Adam that I wouldn't wish to be on his bad side, he instantly agrees. This is what is needed when you are on the forefront, fighting to save our world. You need conviction, crossed with a frustration and a drive to make a difference. When you see what needs be done in the world, it can be frustrating when you come up against the ignorance of others. It takes a strong soul to keep putting out the message, knowing that one day the others will wake up.
Established in 1977, Sea Shepherd is an international non-profit, marine wildlife conservation organization. Their mission is to end the destruction of habitat and slaughter of wildlife in the world's oceans in order to conserve and protect ecosystems and species. Sea Shepherd works to ensure their survival for future generations.
Adam explained that "the awareness of whats happening in the oceans has increased, we have been doing this market for the past 8 years. I have noticed an amazing change unfold over those 8 years, most kids come up to the whale poster and are now able to identify all of the whales." "Awareness is increasing!" "Yesterday Iceland announced they would stop whaling, our volunteers sank two of their ships in 1966, it took them out of business for 16 years. Due to the awareness we have brought, the Japanese public is no longer eating whale meat. The whale meat was collecting in their warehouses, it become an economic nightmare. This is a great victory. The last poaching ship vessel in Southern ocean Antartica has finally been caught, that campaign has been finished." Adam went on to say, "many people are drawn to Sea shepherd because of the "cute whales and creatures. Sea shepherd is so much more. It is about saving our oceans, without our oceans we are without a planet. "If the whales are the beacon for people to save the planet, then great."
Adams conservation work started at Terranium creek in 1979. Known as the 'big scrub' originally, this massive forest dwindled down to this tiny forest, all of the hippies got together and stopped it. This is one my most favourite things about this community, people are so connected to the land and themselves, they innately know right from wrong. Rather than be told to conform, sit down and say nothing. Go back to work, consume, watch, die! These amazing custodians band together, step up and do the work for all of us.
Adam finishes by saying, "We are thinking of opening opening a Land, Sea shepherd, 'Land Shepherd'." Who knows, I would not be surprised, this organisation is a powerful force around the world. Volunteers from all over, risk their lives to save ours. The ultimate act of service.
Usually we ask our souls, how they want to be listed in the book. Adam did not want to be known as Adam, His words, "This is not about Adam. This is about Sea shepherd. This is all about saving our planet! Aho dear soul!
← it doesn't interest me...chrysalis..... →


A small area of still water, typically one formed naturally.
A form of billiards in which the object is to pocket all the balls with a cue ball. There are many versions of the game, e.g. straight pool or eight ball.
A group of contestants who compete against each other in a tournament for the right to advance to the next round.
An arrangement, illegal in many countries, between competing parties to fix prices or rates and share business in order to eliminate competition.


No Public Policy Violation in Dual Recovery Exclusion; Estate may not double dip for liability and u | Truck Accident Lawyer Group Inc.
The estate of a man killed in a single-vehicle accident may not recover liability and underinsured motorist benefits from the same policy, a federal district judge has ruled.
While the estate argued that the decedent had no direct control over the amount of liability coverage on the policy held by his father, U.S. District Judge John E. Jones III said this is a distinction without a difference.
To now award Brent's estate UIM benefits, in addition to $57,500 in liability coverage, would violate not only the express terms of Charles D. Leiter's policy with the Allstate Insurance Co. but also the public policy underlying the Motor Vehicle Financial Responsibility Law, Jones concluded.
The case was filed as a declaratory judgment action in June 2003, in which Allstate asked the U.S. District Court for Middle Pennsylvania to declare that at the time of the accident Brent Leiter's vehicle did not meet the definition of an underinsured vehicle under Charles Leiter's policy.
Brent and a friend, Andrew J. Guizio, were traveling in Brent's Chevrolet Baretta on March 11, 2001, when they were involved in a single-vehicle accident, according to Jones' opinion. Both men were killed in the crash. While it was unclear who was driving at the time of the accident, Brent's estate contended that Guizio was driving, while Guizio's estate argued that Brent was driving.
The Leiters' policy provided $100,000 in bodily injury liability and $100,000 in UIM coverage for four vehicles, including Brent's Baretta, the opinion stated. In April 2002, Allstate tendered the $100,000 bodily injury limit to both estates. By the terms of a mediated settlement, $57,500 was awarded to Brent's estate, and $42,500 to Guizio's, according to the opinion.
After the settlement, Brent's estate filed a UIM claim, which Allstate denied, prompting the court action.
Ruling on Allstate's motion for judgment on the pleadings, Jones agreed with the insurer that two exclusions under the Leiters' policy precluded UIM coverage for Brent's Baretta in this case.
Acknowledging that the exclusions preclude coverage for the Baretta in this case, the estate nonetheless argued that UIM benefits are available for the three remaining vehicles under the policy. Moreover, the estate argued, even if the terms of the policy preclude UIM coverage, such exclusions violate the public policy of the MVFRL.
Citing the dual recovery exclusion, Jones first rejected the estate's argument that UIM benefits are available under the three vehicles not involved in the accident.
Considering the public policy arguments, Jones turned to state appellate precedent to ascertain the underlying policy of the MVFRL.
Citing the Pennsylvania Superior Court's decision in Kelly v. Nationwide Insurance Co., 606 A.2d 470 [Pa. Super. 1992], Jones noted that statutory law governing UIM coverage suggests that such benefits are available only when an accident involves two or more vehicles with separate policies. One policy provides the source of liability coverage, which is insufficient to compensate the victim, while the other, usually held by the injured party not at fault, provides the source of UIM coverage.
"Recognizing that liability insurance is the most expensive form of insurance coverage in Pennsylvania and seeking to prevent insureds from converting less expensive UIM insurance into the more expensive liability insurance, Pennsylvania courts have repeatedly held that in cases involving single tortfeasor accidents, dual recovery of UIM benefits and liability benefits under the same contract are impermissible," Jones explained.
In Kelly, the Superior Court rejected a challenge to the public policy underlying dual recovery exclusions. In that case, the plaintiff was involved in a single-vehicle accident, in which her husband was driving, and had recovered the policy's liability limit for bodily injury. She argued that the UIM exclusion should be invalidated because "in substantive terms her factual position is identical to that of a separate policyholder" - her husband.
In the present case, Brent's estate attempted to distinguish its case from the Kelly ruling, arguing that neither Brent nor the named insured had control over the amount of liability coverage secured by the professed tortfeasor in this case, Guizio.
Counsel for the estate, John R. Bonner of Casale & Bonner in Williamsport, said his clients had no intention of appealing the judge's ruling.


Mom vs Dad: Who works harder?
Mom vs Dad: Who works harder? Okay, so, I know that it's a pretty general question. I'm sure that there are a bunch of different responses that can come from it, since each person's situation is unique. And the truth is I wouldn't even be thinking these thoughts if I hadn't just had the day that I've…uhh… just had.
Anyways, I digress, a few hours later, Hubstopher was up for work, and I decided to stay at home with Curly. The day passed in a blur… doctors visit, trying to get this temperature to behave, washed bedding and clothes, attempted to wash my hair as quick as I can (if you could even call it that). Then her tummy started acting up too, so I've had to wash pillows and clean messes up from the floor (spillage)… it's been fun and games guys… fun and games. And as it nears 5pm I am filled with thoughts of: "but wait, your dad will be home soon!" I mean, hello, it IS his turn after all… right? Lucky ol' him got to spend the whole day at work, while I stayed at home to soothe, clean up and give medicine.
I guess this is where compromise comes in. It's a little give, a little take and a whole lot of "but how can I help you?" action that needs to be going on. Consideration makes the world go round and the home a happy place……. Must remember that when I'm cleaning the puke out of my hair later.
Is it weird that I want to adopt?


2005 Larson 370 Cabrio express cruisers for $92,450 (or best offer).
This vessel is located in Stuart, Florida and is in great condition. She is also equipped with twin Volvo Penta engines that have 430 hours.
Take a look at ALL ***183 PICTURES*** of this vessel, AND A VIDEO, on our main website at POPYACHTS DOT COM. We appreciate that you took your time to look at our advertisement and we look forward to speaking with you!


that includes ashtray, box of matches and cigar cutter.
Price includes Tax, Gratuity & $2 processing fee. Any food/drink/gratuity purchases beyond the brunch (with wristband) are solely the responsibility of the purchaser.
All sales are final. If you are unable to attend the event and would like to offer your ticket to someone else, please send an email to rescuepoetix@gmail.com with the name and contact information. Any name/attendee changes are subject to email confirmation.
Cancellations and no shows are not subject to refund or reschedule.