text:
Innate
A blog about Electronic Music, Art & Media
Owain K
A short selection of music related books to add to your reading list
How Music Works
A remarkable celebration of a subject he has spent a lifetime living, breathing and thinking about, 'Talking Heads' frontman David Byrne explores how music is profoundly shaped by the time and place it originates from. He examines how recording technology in the twentieth century has forever changed our relationship to playing, performing, and listening. Seeing music as part of a larger pattern of adaptations and responses to cultural and physical context, Byrne covers a wide range of ground taking you from Opera ouses to African villages, from his earliest high school reel-to-reel recordings to his latest work in a home music studio (and everything in between). 'How Music Works' is the result of some deep insight and lived experience – an irresistible adventure about music's life-affirming power.
Ambient Century: From Mahler to Moby – The Evolution of Sound in the Electronic Age
Mark Prendergast
If the title is anything to go by, 'The Ambient Century' is a book that rightly covers a lot of ground. Predergast narrates the drift in twentieth-century music from composers to non-musicians and how the rules have seemingly been re-written including the advent of single note repetition to the widespread use of samples. Exploring the classical horizons of Mahler, Satie and Debussy to the revolution in electronics that was first spawned by Stockhausen and Cage – there is a rich wealth of information and comment brought together as he moves through the decades and genres. From the minimalism of Phillip Glass and Terry Riley, to the worlds of Brian Eno and Arvo Part, not withstanding the defining music of rock legends that were The Beatles and Jimi Hendrix to the creations of Kraftwerk and Goldie. The author traces one of the most important developments in music of the past century and examines it in place.
Lost and Sound: Berlin, Techno und der Easyjetset
Tobias Rapp
Since being published in 2009, 'Lost and Sound' has become a bit of a modern classic in terms of capturing the spirit of the moment. A reporter for Berlin's 'Die Tageszeitung' covering the city's nightlife, Tobias Rapp is one of those people who is well suited to write about Berlin and the development of the techno. An interesting analysis and read for anyone wanting to learn about how the city's much coveted music scene has moved from the underground to the mainstream: 'Lost and Sound' maps the key changes in recent times. Rapp focuses on four aspects of change – the move of the scene's focus from Friedrichshain to Kreuzberg, the ageing of active club-goers, incoming techno tourists and the replacement of dark, hard sounds with lighter touch techno pop.
Altered State: The Story of Ecstasy Culture and Acid House
As books about Acid House culture in the UK go, "Altered State" is widely considered as a definitive account of dance culture. Matthew Collin casts his eyes on the heady events of the acid house 'Summer of Love' and the rave scene's euphoric escalation into commercial excess. With an assembled cast of memorable characters helping track the origins of the scene from the burgeoning underground gay discos to the Balearic paradise of Ibiza – Altered State examines the ideologies and myths: documenting both the criminal underside next to the blissed-out image portrayed to many. As accounts go, the book sheds new light on the social history of one the most spectacular youth movements of the twentieth century.
Love Saves the Day: A History of American Dance Music Culture, 1970-1979
As books and authors go, Tim Lawrence's "Love Saves the Day" is one of those must read moments for anyone who has an interest in uncovering the roots of club music and culture as we know it. The book captures intimately the music that America tried to forget: Disco, following the journey with a detailed analysis of the era's most influential DJs and the venues in which they played. Opening with David Mancuso's seminal Love Saves the Day Valentine's party, Tim Lawrence tells the definitive story of American dance music culture in the 1970s. From the very depth of its roots in NoHo and Hell's Kitchen, to its takeover of midtown Manhattan and wildfire transmission through America's suburbs and hotspots such as Chicago, Boston, San Francisco, Los Angeles and Miami. "Love Saves the Day" includes rafts of material and interviews from John 'Jellybean' Benitez, Steve D'Acquisto, Francis Grasso, Loleatta Holloway, Francois Kevorkian, Frankie Knuckles, David Mancuso, Vince Montana, Giorgio Moroder, Tom Moulton, Nicky Siano, Judy Weinstein, and many more.
B-Sides (Part 5): Forgotten gems, a lost art? Pleasures from Planet E On Video Innate Playlist 003: Techno and dubbed out discs from Rick Hopkins
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text:
American basketball player
For the American electric blues guitarist and singer, see Willie D. Warren.
No. 13 – Taoyuan Pilots
Point guard / Shooting guard
P. LEAGUE+
(1989-10-22) October 22, 1989 (age 31)
Dallas, Texas
North Crowley (Fort Worth, Texas)
Oklahoma (2008–2010)
NBA draft
2010 / Round: 2 / Pick: 54th overall
Selected by the Los Angeles Clippers
Los Angeles Clippers
→Bakersfield Jam
Rio Grande Valley Vipers
Maccabi Rishon LeZion
Szolnoki Olaj
Virtus Bologna
Chongqing Fly Dragon
Zhejiang Golden Bulls
Petrochimi Bandar Imam
Shanxi Brave Dragons
Texas Legends
Hoops Club
Sporting Al Riyadi Beirut
CBA All-Star (2016)
Second-team All-Big 12 (2009)
Big 12 Freshman of the Year (2009)
Big 12 All-Rookie team (2009)
McDonald's All-American (2008)
Third-team Parade All-American (2008)
Texas Mr. Basketball (2008)
Stats at NBA.com
Willie D. Warren (born October 22, 1989) is an American professional basketball player for the Sporting Al Riyadi Beirut of the Lebanese Basketball League. He played college basketball for Oklahoma.
Willie Warren career highlights mix
Willie Warren State Championship Highlights
Willie "Fingernail Will" Warren
1 High school career
2 College career
3 Professional career
4 NBA career statistics
4.1 Regular season
High school career
After a successful high school career at North Crowley High School, Warren was selected to be a McDonald's All-American.
Considered a five-star recruit by Rivals.com, Warren was listed as the No. 4 point guard and the No. 10 player in the nation in 2008.[1]
College career
Warren then went to the University of Oklahoma where he played on the same team as his eventual Clippers teammate, Blake Griffin. Despite being a projected lottery pick after a successful Freshman year, Warren stayed at OU, hoping to be the primary option with Griffin leaving for the draft. Throughout an injury-plagued Sophomore year he averaged over 16 points, 4 assists, 3 rebounds and just over 1 steal a game while shooting above 30% from 3-point range . At the end of his sophomore season, Warren decided to forgo his last two years of eligibility, signing with an agent and declaring for the 2010 NBA draft.
Warren was drafted late in the 2nd round as the 54th overall by the Los Angeles Clippers on the day of the 2010 NBA draft. Then, on July 13, 2010, unlike many other late second-round picks, he was signed by the Clippers.[2]
Late in the 2010–11 season, Warren was assigned to the NBA D-League. There, he played 6 games in 2011 for the Bakersfield Jam of the NBA D-League where he averaged 21.0 points, 7.0 assists, 5.3 rebounds and 1.0 steals per game while shooting above 50 percent from the field and 46 percent from 3-point range. The Jam went 5-1 with Warren in the lineup from February 4 – 16. After the D-League stint, Warren was called back into the Clippers' line-up, but he was later reassigned for a second stint on March 2, 2011.[3] On December 19, 2011, Warren was waived by the Clippers.[4]
In August 2012, Warren signed with Maccabi Rishon LeZion of the Israeli Super League for the 2012–13 season.[5]
In the summer of 2013, he signed with Szolnoki Olaj KK of Hungary.[6] On February 28, 2014, he was waived by Szolnoki.[7] On March 4, 2014, he signed with Virtus Bologna of Italy for the rest of the 2013–14 Lega Basket Serie A season.[8]
After playing for the Chongqing Fly Dragon during the 2014 NBL season, he signed with the club on September 18, 2014 for their inaugural season in the Chinese Basketball Association.[9] On April 16, 2015, he signed with Club Sagesse of the Lebanese Basketball League.[10] However, he never joined the Lebanese team due to an injury.[11]
On August 6, 2015, Warren signed with Zhejiang Golden Bulls for the 2015–16 season.[12] He later re-signed with Zhejiang for one more season.
On August 13, 2017, Warren signed with Petrochimi Bandar Imam of the Iranian Basketball Super League.[13]
On December 9, 2017, Warren signed with the Shanxi Brave Dragons of the Chinese Basketball Association.[14]
On February 22, 2018, Warren was acquired by the Texas Legends,[15] but was waived on March 23 after playing three games.[16]
On June 30, 2019 he has signed with Sporting Al Riyadi Beirut of the Lebanese Basketball League.[17]
GP Games played GS Games started MPG Minutes per game
FG% Field goal percentage 3P% 3-point field goal percentage FT% Free throw percentage
RPG Rebounds per game APG Assists per game SPG Steals per game
BPG Blocks per game PPG Points per game Bold Career high
2010–11 L.A. Clippers 19 0 17.1 .371 .333 .750 .6 1.4 .3 .0 1.9
Career 19 0 17.1 .371 .333 .750 .6 1.4 .3 .0 1.9
^ Willie Warren Recruiting Profile
^ Los Angeles Clippers sign 2nd round draft pick Willie Warren of Oklahoma[dead link]
^ "NBA Development League: Clippers Assign Willie Warren To Bakersfield Jam". Archived from the original on 2012-02-13. Retrieved 2012-11-05.
^ CLIPPERS: CLIPPERS WAIVE WARREN, AHEARN, KOCH AND MAJOR
^ Maccabi Rishon Lezion signs American PG Willie Warren
^ A Szolnoki Olaj Kosárlabda Klub a mai napon egyéves szerződést kötött Willie Warren-nel.
^ "Szolnoki Olaj waive Willie Warren". Sportando.net. Retrieved 28 February 2014.
^ "Granarolo Bologna announces Willie Warren until 2015". Sportando.net. 4 March 2014. Retrieved 25 March 2014.
^ Willie Warren signs with Chongqing Fly Dragon
^ Willie Warren agreed to terms with Sagesse
^ "Sagesse signs Brandon Heath". Archived from the original on 2017-06-20. Retrieved 2015-04-22.
^ Willie Warren inks with Zhejiang Chouzhou
^ "Mirza Begic and Willie Warren ink in Iran with Petrochimi". Sportando.com. August 13, 2017. Archived from the original on September 17, 2017. Retrieved August 13, 2017.
^ "山西官宣:因成绩不理想更换外援 沃伦替詹宁斯". Sina.com (in Chinese). December 9, 2017. Retrieved December 10, 2017.
^ "Legends Complete Trade with Clippers". OurSportsCentral.com. February 22, 2018. Retrieved April 1, 2018.
^ "Texas Legends waive Willie Warren". Sportando.com. March 7, 2018. Retrieved April 1, 2018. [permanent dead link]
^ "Willie Warren signs with Al Riyadi". Sportando. June 30, 2019. Retrieved July 1, 2019.
Career statistics and player information from NBA.com or Basketball-Reference.com
Oklahoma Sooners bio
FIBA.com profile
2010 NBA draft
John Wall
Evan Turner
Derrick Favors
Wesley Johnson
DeMarcus Cousins
Ekpe Udoh
Greg Monroe
Al-Farouq Aminu
Gordon Hayward
Paul George
Cole Aldrich
Xavier Henry
Ed Davis
Patrick Patterson
Larry Sanders
Luke Babbitt
Kevin Séraphin
Eric Bledsoe
Avery Bradley
James Anderson
Craig Brackins
Elliot Williams
Trevor Booker
Damion James
Dominique Jones
Quincy Pondexter
Jordan Crawford
Greivis Vásquez
Daniel Orton
Lazar Hayward
Tibor Pleiß
Dexter Pittman
Hassan Whiteside
Armon Johnson
Nemanja Bjelica
Terrico White
Darington Hobson
Andy Rautins
Landry Fields
Lance Stephenson
Jarvis Varnado
Da'Sean Butler
Devin Ebanks
Jerome Jordan
Paulão Prestes
Gani Lawal
Tiny Gallon
Latavious Williams
Ryan Richards
Solomon Alabi
Magnum Rolle
Luke Harangody
Pape Sy
Willie Warren
Jeremy Evans
Hamady N'Diaye
Ryan Reid
Derrick Caracter
Stanley Robinson
Dwayne Collins

redpajama_set_name: RedPajamaCommonCrawl


45% of Dutch consumers prefer to shop in a 'charming' physical store than online. These are the results of an international survey of Mood Media, which was filled out by a thousand Dutch people.
Using the appropriate combination of music, visuals and smell, 40% of respondents wants to stay longer in a shop. 56% says they will come back sooner if the atmosphere is nice, while 22% say those elements have no influence at all. In addition 27% say the atmosphere is the main reason to visit physical stores, this is well above the international average of 20%.
Fitting and touching of physical products is by far the main reason to go to shops by Dutch consumers: 76%. Followed by direct contact with a product and the ability to discover new products, with respectively 60% and 49%.
49% of Dutch consumers would like to receive deals on their phone in the shop. Among 18-to 24-year-olds this is even 74%. As the age increases this aspect is less important. Worldwide 53% of consumers like to receive offers on their smartphones while shopping.


text:
Development/Plasticity/Repair
Early Serotonergic Projections to Cajal-Retzius Cells: Relevance for Cortical Development
Skirmantas Janušonis, Vicko Gluncic and Pasko Rakic
Journal of Neuroscience 18 February 2004, 24 (7) 1652-1659; DOI: https://doi.org/10.1523/JNEUROSCI.4651-03.2004
Skirmantas Janušonis
Vicko Gluncic
Pasko Rakic
Although the serotonergic system plays an important role in various neurological disorders, the role of early serotonergic projections to the developing cerebral cortex is not well understood. Because serotonergic fibers enter the marginal zone (MZ) before birth, it has been suggested that they may influence cortical development through synaptic contacts with Cajal-Retzius (CR) cells. We used immunohistochemistry combined with confocal and electron microscopy to show that the earliest serotonergic projections to the MZ form synaptic contacts with the somata and proximal dendrites of CR cells as early as embryonic day 17. To elucidate the functional significance of these early serotonergic contacts with CR cells, we perturbed their normal development by injecting pregnant mice with 5-methoxytryptamine. Lower reelin levels were detected in the brains of newborn pups from the exposed animals. Because reelin plays an important role in the cortical laminar and columnar organization during development, we killed some pups from the same litters on postnatal day 7 and analyzed their presubicular cortex. We found that the supragranular layers of the presubicular cortex (which normally display a visible columnar deployment of neurons) were altered in the treated animals. Our results suggest a mechanism of how serotonergic abnormalities during cortical development may disturb the normal cortical organization; and, therefore, may be relevant for understanding neurological disorders in which abnormalities of the serotonergic system are accompanied by cortical pathology (such as autism).
serotonin (5-HT)
Cajal-Retzius cells
microcolumns
marginal zone
The serotonergic system plays an important role in various developmental neurological disorders (Azmitia, 2001; Whitaker-Azmitia, 2001); however, the role of early serotonergic projections to the developing cortex before birth is not well understood. Previous light microscopy studies have shown that, after reaching the neocortex, serotonergic afferents form two bands, one in the marginal zone (MZ) above the cortical plate and the other below the cortical plate, before they massively invade the cortical plate itself (Lidov and Molliver, 1982; Wallace and Lauder, 1983; Bruning et al., 1997; Verney et al., 2002). In the mouse and rat, the first serotonergic fibers in the developing neocortex appear around embryonic day 16-17 (E16-17) (Wallace and Lauder, 1983; Bruning et al., 1997). Because early serotonergic projections target the MZ, it has been suggested that they may play an important role in cortical development (Whitaker-Azmitia et al., 1996; Persico et al., 2003). Specifically, it has been proposed that they may form contacts with Cajal-Retzius (CR) cells (Radnikow et al., 2002), but no actual synaptic contacts have so far been demonstrated. CR cells are located in the MZ and are considered to be necessary for the correct positioning of neurons in cortical layers (Ogawa et al., 1995; Marín-Padilla, 1998; Dulabon et al., 2000; Hack et al., 2002; Ang et al., 2003; Rakic and Zecevic, 2003), as well as in radial columns (Nishikawa et al., 2002; Luhmann et al., 2003). Because microcolumns are basic anatomical and functional units of the cerebral cortex (Jones, 2000; Buxhoeveden and Casanova, 2002), their abnormalities underlie several neurological disorders in humans. Specifically, the evidence that the serotonergic system is disturbed in several debilitating neurological disorders, such as autism and schizophrenia (Anderson et al., 1990; Chugani et al., 1999; Chugani, 2002; Dean, 2003), has been recently complemented by reports of cortical columnar pathology in some of these disorders (Casanova et al., 2002a,c). Interestingly, the levels of reelin, a glycoprotein produced by CR cells, are also abnormal in some of these disorders (Fatemi et al., 2001a,b, 2002; Fatemi, 2002; Eastwood and Harrison, 2003).
Based on these studies, we hypothesized that an abnormal serotonergic input to CR cells may have an effect on the late stages of embryonic cortical development. To test this hypothesis, we first investigated whether serotonergic afferents to the MZ make synapses with CR cells, and, second, whether manipulation of the serotonin system can affect reelin expression and lead to anatomical disturbances of the mouse cerebral cortex.
Animals. Timed-pregnant mice (CD-1) were obtained from Charles River Laboratories (Wilmington, MA) and housed in a mouse facility until used for experiments. All procedures have been approved by the Yale University Animal Care and Use Committee and were in compliance with the Policies on the Use of Animals and Humans in Neuroscience Research approved by the Society for Neuroscience.
Fluorescent confocal microscopy. Brains of E17 embryos were extracted and immersion-fixed in 4% paraformaldehyde overnight. They were cryoprotected in 30% sucrose, embedded in Tissue-Tek O.C.T. compound (Sakura Finetek, Inc., Torrance, CA) and cut coronally on a cryostat at 30-40 μm. The sections (on slides or free-floating) were then processed with immunohistochemistry. They were rinsed in 0.1 m PBS, pH 7.4, blocked in 3-5% normal donkey serum (NDS) and 0.5-1% Triton X-100 in PBS, and incubated in 1:200 rabbit anti-5-HT (catalog #NT102; Protos Biotech Corporation, New York, NY) and 1:100 mouse anti-reelin (a gift of Dr. M. Ogawa, RIKEN Brain Science Institute, Wako City, Japan) antibodies in the blocking solution for 1-3 d at 4°C. They were rinsed in PBS, incubated in 1:100 FITC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA) and 1:100 Cy3-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch) in the blocking serum, and rinsed in PBS. Free-floating sections were mounted on slides and air-dried. Sections were cleared with xylenes, coverslipped, and examined with an epifluorescent microscope (Axioplan 2; Zeiss, Jena, GmBH) and a confocal microscope (LSM 510; Zeiss, Jena, GmBH).
Electron microscopy. Pregnant mice (E17) were deeply anesthetized and killed by cervical dislocation. Their embryos were quickly extracted from the uterus and transcardially perfused under a stereomicroscope with chilled 4% formaldehyde (Polysciences, Warrington, PA) containing 0.2-3.0% glutaraldehyde (a perfusion system with a 30-gauge needle was used). The brains were immediately dissected from the skulls in the same fixative and postfixed in the same fixative overnight at 4°C. After postfixation, the brains were embedded in 3% agarose at 39°C, cut on a vibratome at 50 μm thickness into 0.1 m phosphate buffer with ice cubes, and immediately processed with 5-HT immunohistochemistry. Specifically, the sections were rinsed in PBS, pH 7.4, and some of them were pretreated with 1% sodium borohydride in PBS for 30 min, followed by rinses in PBS. The sections were then incubated in 0.3% H2O2 in PBS for 20 min, rinsed in PBS, and blocked in a solution containing 5% normal goat serum, 1% bovine serum albumin, 0.1% glycine, and 0.1% l-lysine in PBS for 1 hr. The sections then were incubated in a rabbit anti-5-HT antibody (1:1000; Protos Biotech Corporation) in the blocking solution at 4°C overnight. They were rinsed in PBS, incubated in 1:200 biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA) in the blocking solution for 90 min, rinsed in PBS, incubated in 1:100 ABC (Vector) in PBS for 1 hr, and rinsed in PBS, followed by a few rinses in 0.05 m Tris buffer (TB), pH 7.4. They were developed in 0.05% 3,3′-diaminobenzidine (DAB) with 0.04% NH4Cl, 0.016 mg/ml glucose oxidase (catalog #G-2133, type III; Sigma, St. Louis, MO), and 0.2% β-d-glucose in TB for 5 min. They were rinsed in TB, osmicated with 1% OsO4 in TB for 1 hr, rinsed in TB, and stored overnight at 4°C. They were then dehydrated through a series of graded ethanols, lightly contrasted with uranyl acetate, and embedded in Durcupan (Electron Microscopy Sciences, Fort Washington, PA). Punches containing DAB-labeled fibers in the MZ were excised, ultrasectioned at 70 nm onto single-slot grids, and examined with a JEOL (Tokyo, Japan) 1010 electron microscope.
5-Methoxtryptamine injections. The embryonic serotonergic system was perturbed by using a procedure developed by Shemer et al. (1991) and Kahne et al. (2002). Starting at E12, timed-pregnant mice were injected (1 mg/kg, s.c.) with 5-methoxytryptamine (5-MT; catalog #M7002; Sigma), which was dissolved in bacteriostatic saline at 0.25 mg/ml and stored in aliquots at -20°C. Control mice were injected with the same volume of bacteriostatic saline. The injections were done once a day every day until the mice delivered.
Western blotting. The brains of two newborn pups (P0) from each litter were quickly extracted and homogenized on ice with a pestle rotating at 2000 rpm in a 2 ml glass tube with 0.5 ml of a homogenization buffer containing 25 mm HEPES, 150 mm KCl, and the EDTA-free protease inhibitor cocktail (Roche, Mannheim, Germany) in 0.05 m Tris buffer, pH 7.4. The homogenate was centrifuged at 3000 × g for 15 min at 4°C, and the supernatant was centrifuged again at 3000 × g for 10 min at 4°C. The collected supernatant (the final lysate) was stored at -80°C.
The blood of P0 pups was collected into EDTA-tubes (on ice). The blood of five pups from the same litter was collected into each tube. The blood samples were centrifuged in an Eppendorf microcentrifuge at 10,000 rpm for 30 min at 4°C, and the supernatant was centrifuged again at 14,000 rpm for 10 min. The serum supernatant was stored at -80°C.
The protein amounts in samples were estimated with a BCA Protein Assay reagent kit (Pierce, Rockford, IL). Brain lysates were thawed out on ice, and the loading samples were prepared by adding 50 μl of 2× SDS sample buffer, 1 μl of 2-mercaptoethanol, and bromophenol blue to 50 μl of each lysate. Samples were boiled for 3 min, and 60 μg of protein (∼23 μl) was loaded onto each lane of a 7.5% Tris-HCl pre-cast minigel (Bio-Rad Laboratories, Hercules, CA). Full Range Rainbow protein molecular weight markers (catalog number RPN800; Amersham Biosciences, Piscataway, NJ) were run in a few lanes of the gel. The proteins were separated by SDS-PAGE electrophoresis run at 200 V for 1 hr at room temperature. The membrane then was rinsed for 5 min in transfer buffer, and proteins were electroblotted onto an Immobilon-P polyvinylidene difluoride (0.45 μm) membrane (Millipore, Bedford, MA) at 100 V (first hour) and 75 V (second hour) in packed ice in a cold room. The transfer was assessed with the Ponceau S solution. The membrane was allowed to dry overnight and was immunoprobed for reelin. It was blocked in 5% dry milk in PBS, incubated with a mouse anti-reelin IgG1κ (G10; catalog #MAB5364, diluted 1:100; Chemicon, Temecula, CA) in the blocking solution for 2 hr at room temperature on a rocker, rinsed four times (5 min each) in PBS with 0.2% Tween 20 (PBST), incubated in 1:10,000 HRP-conjugated donkey anti-mouse IgG (Jackson ImmunoResearch) in the blocking solution for 1 hr, rinsed well in PBST followed by a few rinses in PBS, and reacted with the SuperSignal West Pico chemiluminescent solution (Pierce, Rockford, IL). The reelin bands were visualized with Hyperfilm ECL (Amersham Pharmacia Biotech, Buckinghamshire, UK).
Blood serums were diluted 1:5 with the homogenization buffer before adding 2× SDS sample buffer, 2-mercaptoethanol, and bromphenol blue. The prepared samples were boiled for 2 min, and the separated proteins were electroblotted at 100 V for 2 hr in a cold room. All the other procedures were the same as for the brain lysates.
Postnatal Nissl staining. For Nissl staining, pups (postnatal day 7) were perfused with chilled 4% paraformaldehyde. Their brains were extracted, postfixed in 4% paraformaldehyde at 4°C overnight, immersed in 30% sucrose at 4°C overnight, embedded in 20% gelatin, cut coronally on a freezing microtome at 50 μm thickness, mounted on slides, stained with thionin, dehydrated through a series of graded alcohols, and coverslipped.
At E17, two streams of serotonin-immunoreactive (5-HT+) fibers entered the anterior neocortex through the septum (Fig. 1A). In coronal sections, they were visible as two 5-HT+ bands: one in the MZ and the other below the cortical plate which, itself, was almost devoid of serotonergic fibers. The 5-HT+ fibers had strongly immunoreactive varicosities connected with less immunoreactive intervaricose segments. These fibers were more loosely dispersed in the deep band than in the MZ, where they appeared to be confined to this narrow layer. Some 5-HT+ fibers crossed the cortical plate, but all of them ascended from the deep band. In contrast, we observed no 5-HT+ fibers descending from the MZ to the deep band. Typical ascending fibers were strongly immunoreactive, did not branch, and often reached CR cells in the MZ (Fig. 1B). Confocal microscopy and three-dimensional analysis of obtained optical sections showed that ascending 5-HT+ fibers contacted CR cells (Fig. 1C). Some tangentially oriented fibers in the MZ also contacted CR cells (Fig. 1D), but we could not determine whether they belonged to the MZ band or were long tangential extensions of fibers that reached the MZ from the deep band.
Serotonergic afferents (FITC, green) contact CR cells (Cy3, red) in the MZ of E17 embryos. A, Serotonergic fibers enter the developing cortex and form two bands, one in the marginal zone (MZ) and the other in the subplate (SP). B, Some serotonergic fibers in the SP traverse the cortical plate to reach the MZ. C, Confocal microphotograph of serotonergic fibers contacting CR cells in the MZ. The arrow indicates a point of contact confirmed by rotating the image in 3D. D, Horizontal serotonergic fibers contacting CR cells in the MZ. Scale bars: A, 200 μm; B, 100 μm; C, D, 20 μm.
To further investigate the contacts between 5-HT+ fibers and CR cells, we immunolabeled cortical serotonergic afferents with 3,3′-diaminobenzidine (DAB) and analyzed them at the electron microscopy level. Immunoelectron microscopy of the embryonic mouse brain presents considerable challenges, because good preservation of the tissue is hard to achieve because of a large content of water, and high concentrations of glutaraldehyde suppress or completely abolish specific immunoreactivity. Therefore, to optimize our experiments, we analyzed the ultrastructure and 5-HT immunoreactivity of E17 brains fixed with 4% paraformaldehyde containing a range of glutaraldehyde concentrations (0.2-3.0%). Also, some brain sections were pretreated with sodium borohydride to recover some lost antigenicity. We found that 1% glutaraldehyde and pretreatment with sodium borohydride gave the optimum tissue preservation without abolishing immunoreactivity.
At E17, DAB-labeled (5-HT+) profiles were observed in the MZ and in the cortical plate (only the superficial cortical layers were analyzed). We assumed that the large, strongly 5-HT-immunoreactive profiles containing vesicles were serotonergic varicosities, as opposed to the thin and less immunoreactive intervaricose segments. This interpretation has been used by other researchers (Papadopoulos et al., 1987; Smiley and Goldman-Rakic, 1996; Paspalas and Papadopoulos, 2001; Miceli et al., 2002). CR cell somata could be recognized by their position, elongated profiles oriented parallel to the pia, and by the characteristic features of their cytoplasm and nucleus (Derer et al., 2001). Most profiles of 5-HT+ fibers were observed in the top part of the MZ (close to the pial edge of the MZ), containing the somata of CR cells (Fig. 2). Also, we frequently observed 5-HT+ fibers at the very pial edge of the MZ, "above" the somata of CR cells, where other somatic or dendritic profiles were very sparse or completely absent. It is possible that these fibers approached CR cells from the pial side, perhaps looping around them, as can be seen in a confocal image (Fig. 1D). Some 5-HT+ varicosities made putative synaptic contacts with the pial side of CR cell somata (Fig. 2C,D). Although heavy immunoprecipitation in these varicosities partly obscured their ultrastructure, we could measure some individual vesicles that were ∼40 nm in diameter. The postsynaptic elements of CR cells had no visible electron-dense thickening. These axo-somatic contacts were relatively rare: we found no more than one such putative synapse in serial ultrathin sections obtained from a 50-μm-thick section. In contrast, 5-HT+ varicosities contacting dendrites of CR cells were more prevalent (Fig. 3). These contacts again appeared to be immature or specialized symmetric synapses with no postsynaptic thickening. Some of the presynaptic 5-HT+ varicosities had clearly visible, densely stained small vesicles around 40 nm in diameter and a few large dense-cored vesicles of ∼120 nm in diameter (Fig. 3D). The ultrastructure of some 5-HT+ profiles closely resembled that of serotonergic growth cones, as described by Igvy-May et al. (1994). They contained small and large dense-cored vesicles, as well as large vacuoles (Fig. 3E). Some of these 5-HT+ profiles made contact with finger-like cytoplasmic protrusions of adjacent unlabeled dendrites or somata (Fig. 3E).
Ultrastructure of serotonergic axo-somatic contacts in the MZ of E17 embryos. A, Montage of electron micrographs showing a DAB-labeled (5-HT+) varicosity (arrow) contacting the soma of a CR cell. Note neuronal somata (N) in layers II-III and their processes (arrowheads) extended toward the MZ. B, Schematic representation of A. CR cells are colored in red, neurons in layers II-III in blue. The cells colored in gray may be tangentially migrating GABAergic interneurons, located at a certain distance from the CR cells, as previously demonstrated by Ang et al. (2003). C, Large magnification of the same 5-HT+ varicosity as in A in an adjacent ultrasection. D, Another 5-HT+ varicosity contacting the soma of a CR cell. Scale bars: A, B, 4 μm; C, 250 nm; D, 1 μm.
Ultrastructure of serotonergic axo-dendritic contacts in the MZ of E17 embryos. A, Montage of electron micrographs of a CR cell with adjacent dendrites making contacts with two 5-HT+ varicosities (arrows). The top of the montage corresponds to the pial side of the MZ. B1-B3, In serial ultrathin sections, a 5-HT+ varicosity (indicated with black arrow in A and B1-B3) approaches and makes contact with a putative CR dendrite. C, Higher magnification of the same 5-HT+ varicosity as in B3. D, Contact between a 5-HT+ varicosity (indicated by an open arrow in A and D) and a putative CR dendrite. Note the darkly stained small vesicles and some large dense-cored vesicles in the 5-HT+ varicosity. E, 5-HT+ profile, possibly a growth cone, making contact with an adjacent CR dendrite (top of the panel) and a CR soma (bottom) in the MZ. Note the finger-like cytoplasmic protrusions of the soma (white arrows). Also, note the accumulation of darkly labeled vesicles on the left hand side of the 5-HT+ profile. Scale bars: A, 1 μm; B1-B3, 500 nm; C, 250 nm; D, 200 nm; E, 500 nm.
Having established the presence of contacts between early cortical serotonergic afferents and CR cells, we then asked what functional role they may play. Because CR cells secrete reelin, which is necessary for the normal development of both the laminar and columnar organization of the cerebral cortex, we perturbed the serotonergic system of mouse embryos by injecting pregnant mothers with 5-MT, a nonspecific 5-HT receptor agonist, and we used Western blots to determine reelin levels in their brains at birth (P0). 5-MT is endogenously colocalized with serotonin in many raphe neurons (Patel et al., 1986), and its subcutaneous injections have been previously shown to alter the prenatal serotonergic system and produce pups with behavioral deficits (Shemer et al., 1991; Lauder et al., 2000; Kahne et al., 2002). Two randomly chosen pups from each of the three treated and three control litters were used for brain lysates. In agreement with previous studies in lampreys, rats, mice, and humans (Smalheiser et al., 2000; Perez-Costas et al., 2002), several reelin isoforms were detected in the brain lysates of the mouse pups, with the most intense bands migrating to ∼175, 73, and 37 kDa. We found that the amount of the 175 kDa-reelin isoform was significantly lower in the brains of the pups whose mothers had been injected with 5-MT, as compared with controls (p < 0.05) (Fig. 4).
Reelin levels in the brains of newborn pups prenatally treated with 5-MT. Western blots of P0 brains immunoprobed for reelin (A) and the corresponding densities of the 175 kDa bands in the treated and control animals (B).
Because the manipulation of the serotonergic system altered brain reelin levels, it also could have an effect on the late embryonic stages of cortical laminar and columnar development, which depend on Cajal-Retzius cells and their transient networks (Marín-Padilla, 1998; Nishikawa et al., 2002; Luhmann et al., 2003). To see if supragranular cortical layers were affected in young pups (postnatal day 7) exposed to 5-MT before birth, we analyzed their presubicular cortex, which has visible supragranular columns in early postnatal life (Van Groen and Wyss, 1990; Nishikawa et al., 2002). In Nissl-stained sections, the supragranular layers of the presubicular columns appeared abnormal in pups whose mothers had been injected with 5-MT (Fig. 5). Although some periodical clustering of cells was still preserved, the supragranular columns appeared "smeared" and had cells between the columns, thus partially or fully merging with one another (Fig. 5D,F). These observations were confirmed by counts performed by a trained person blind to the experimental conditions. For each treated and control case, a coronal section with the most prominent presubicular columns was selected, and its image was digitized, coded, and randomly mixed with images from other cases. The columns were counted starting at the most lateral edge of the presubiculum until their darkly Nissl-stained supragranular "caps" merged and could no longer be distinguished as separate around the presubicular-postsubicular border (for the detailed anatomy, see Van Groen and Wyss, 1990). The mean number (± SEM) of the presubicular columns in the treated pups was 6.30 ± 0.37 versus 8.80 ± 0.86 in the control pups (t(8) = -2.665; p < 0.05).
Presubicular cortex of pups (postnatal day 7) prenatally exposed to 5-MT. Nissl staining of the presubicular cortex of P7 pups treated with saline (A, C, E) or 5-MT (B, D, F) during pregnancy (E12 through term). C, D, High magnification of A and B, correspondingly. Note the well defined presubicular columns (arrows) in the saline-treated animals. Scale bars: A, B, 1 mm; C-F, 300 μm.
Several recent studies have suggested that cortical minicolumns are abnormal in several debilitating neurological disorders such as schizophrenia, autism, and dyslexia (Buxhoeveden et al., 2000a; Casanova et al., 2002a,b,c,d; Casanova, 2003) and that reelin levels are altered in the brains and blood of autistic and schizophrenic patients (Fatemi et al., 2000, 2001a,b, 2002). Because our prenatal manipulation of the serotonin system altered brain reelin levels and perturbed postnatal presubicular columns, we were intrigued to see if it also altered reelin levels in blood serum. Consistent with previous reports in rats and humans (Smalheiser et al., 2000), several isoforms of serum reelin were detected, with the most intense bands migrating to ∼293 and 43 kDa. The levels of the 293 kDa isoform were lower in the pups whose serotonergic system had been prenatally disturbed with 5-MT (p < 0.05) (Fig. 6).
Reelin levels in the blood serum of newborn pups prenatally exposed to 5-MT. Western blots of P0 blood serum immunoprobed for reelin (A) and the corresponding densities of the 293 kDa bands in the treated and control animals (B).
In the present study, we found that the earliest serotonergic afferents to the developing cerebral cortex establish synaptic contacts with CR cells and that this serotonergic input may be important for the normal development of the cerebral cortex. The pattern of the prenatal serotonergic innervation and reelin expression by CR cells during cortical development are conserved across mammalian species, including humans (Lidov and Molliver, 1982; Bruning et al., 1997; Smalheiser et al., 2000; Perez-Costas et al., 2002; Verney et al., 2002). Because the prenatal contacts between serotonergic afferents and CR cells are immature and, therefore, lack classical membrane specializations, their exact nature needs further elucidation. Our findings indicate that they are likely to be synaptic, because: (1) the serotonergic varicosities contained small (∼40 nm) and dense-cored vesicles (∼120 nm), which is typical of presynaptic serotonergic varicosities in the adult brain (Paspalas and Papadopoulos, 2001); (2) at least in the adult brain, most (if not all) serotonergic varicosities make overt synapses (Papadopoulos et al., 1987; de Lima et al., 1988; Parnavelas and Papadopoulos, 1989; Papadopoulos and Parnavelas, 1991; Paspalas and Papadopoulos, 2001); (3) even in the adult brain, the active zone of a typical serotonergic synapse is usually small and is often missed unless a complete set of serial sections are examined (Papadopoulos and Parnavelas, 1991). The absence of clear membrane specializations in the present study could also be attributable to the immaturity of the serotonergic synapses and to the loss of membrane integrity, which is difficult to preserve in embryonic brain tissue during fixation. Interestingly, axo-dendritic contacts between serotonergic afferents and CR cells were more frequent than axo-somatic contacts, which is also typical for serotonergic synapses in the rodent cortex (Papadopoulos et al., 1987).
Our results demonstrate that serotonergic afferents establish putative synaptic contacts with CR cells even before neuronal migration is complete and before serotonergic fibers have massively invaded the cortical plate. The early formation of these synapses may be facilitated by the presence of the fully developed presynaptic machinery in serotonergic growth cones (Igvy-May et al., 1994). However, it is not known when and how CR cells can detect serotonergic signals and whether CR cells express serotonin receptors. CR cells express an array of receptors for other neurotransmitters, such as glutamate, GABA, glycine, and norepinephrine (Schwartz et al., 1998; Lu et al., 2001; Radnikow et al., 2002, Luhmann et al., 2003; Soda et al., 2003). The absence of published reports on serotonin receptors on CR cells appears to be attributable to the availability of adequate serotonin receptor antibodies. We have tried and failed to achieve specific labeling in the embryonic and adult brain using commercial 5-HT1A antibodies (Chemicon; Calbiochem) under various perfusion and incubation conditions. However, it is likely that CR cells express 5-HT1A receptors, because (1) early postnatal CR cells hyperpolarize when exposed to serotonin (Foehring et al., 2002), which is typical of the 5-HT1A receptors, and (2) several studies have shown that 5-HT1A receptors exhibit a transient peak in the cortical and cerebellar development perinatally (Bar-Peled et al., 1991; del Olmo et al., 1994), at the approximate time when serotonergic afferents make contacts with CR cells. CR cells may also express other 5-HT receptors, such as the 5-HT3 receptor, as has been proposed by Tecott et al. (1995).
To assess the role of the contacts between serotonergic afferents and CR cells, we manipulated the serotonin system before birth by administering 5-MT, a methylated serotonin derivative, which is also an endogenous trace amine in the brain and a nonspecific 5-HT1 and 5-HT2 receptor agonist (Patel et al., 1986; Yu et al., 2003). Prenatal administration of 5-MT has been shown to affect the sprouting of serotonergic fibers and/or the expression of the serotonin transporter at serotonergic terminals (Shemer et al., 1991) and alter the expression of the 5-HT1A receptor (Lauder et al., 2000). It should be noted that other pharmacological and genetic manipulations of the embryonic serotonergic system are available, such as blocking embryonic 5-HT synthesis with p-chlorophenylalanine (pCPA) (Lauder and Krebs, 1978; Lauder et al., 1985), or knocking out genes that are necessary for the development of serotonergic neurons (Hendricks et al., 2003) or serotonergic cortical projections (Donovan et al., 2002). However, prenatal treatment with 5-MT has the following advantages: (1) 5-MT is endogeneously present in the brain (Patel et al., 1986); (2) its effect on cortical serotonergic fibers is transient and gradually disappears postnatally (Shemer et al., 1991); (3) the injections are easy to make, and treated mice deliver without the delay observed in pCPA-treated animals (Lauder and Krebs, 1978); (4) it mimics the elevated levels of blood 5-HT in autism and produces rat pups that have some "autistic-like" symptoms (Kahne et al., 2002).
In the present study, the manipulation of the prenatal serotonergic system with 5-MT led to altered reelin levels in the brains of newborn pups, with the 175 kDa isoform being less abundant in the prenatally treated pups. Several reelin isoforms of different molecular weight are consistently detected in Western blots in various vertebrate species (Smalheiser et al., 2000; Perez-Costas et al., 2002). These forms appear to be the result of proteolytic cleavage of the full-length reelin molecule (Tissir and Goffinet, 2003). Specific isoforms have been linked to several neurological disorders (Fatemi et al., 2001a,b, 2002; Fatemi, 2002). Because we estimated reelin levels in the whole embryonic brain and because CR cells are not the only cells in the embryonic brain that can express reelin (Alcantara et al., 1998; Nishikawa et al., 1999), it is possible that the expression of reelin by other cells was also altered. However, it is unlikely that the differences in reelin expression in the brain lysates was attributable to contamination with blood reelin because its prevalent isoforms in the brain and serum are distinctly different, as shown previously (Smalheiser et al., 2000) and in the present study. Therefore, we suggest that the 5-MT-induced disruption of the normal serotonergic input to CR cells altered their reelin expression. Because several studies have shown that altered reelin expression in corticogenesis disrupts the normal laminar and columnar organization of the cerebral cortex (Ogawa et al., 1995; Nishikawa et al., 2002), our findings suggest that embryonic serotonergic disturbances may have an impact on the late stages of corticogenesis. Interestingly, it has recently been shown that another monoamine, norepinephrine, also has effects on developing CR cells (Naqui et al., 1999).
The mouse presubicular cortex has been previously used to study the development of the basic columnar organization in the entire cerebral cortex (Nishikawa et al., 1999). Although the presubicular cortex is anatomically distinct from the neocortex (Van Groen and Wyss, 1990), the basic development of its layers is similar to that of the neocortex. In addition, the development of its columns depends on reelin secreted by CR cells (Nishikawa et al., 1999) and, therefore, may be relevant to understanding the development of neocortical microcolumns (they are not readily visible in the mouse cortex). Because we observed abnormal columns in the presubicular cortex of pups whose serotonergic system had been prenatally perturbed, serotonergic signaling to CR cells may play a more important role in cortical development than was previously thought. In the supragranular layers, neighboring columns of treated animals often merged because of ectopic cells between the columns, which is consistent with a report that the intercolumnar spaces of the presubicular cortex correspond to reelin-rich areas in the MZ of the normal mouse (Nishikawa et al., 2002) and with our observation of lower reelin levels in the 5-MT-treated mice. Those relatively subtle alterations in cortical organization are consistent with the fact that serotonergic afferents reach the cortex at the latest stages of cortical development and, because most of the radially migrating neurons have already settled in their final laminar positions, they cannot affect reelin levels early enough to cause a major malformation of cortical layers. However, the latest wave of radially migrating neurons destined for supragranular layers and a significant percentage of tangentially migrating neurons, as well as the onset of the thalamocortical ingrowth, may still be affected. We cannot rule out the possibility that 5-MT treatment had some effect on transient 5-HT uptake by nonserotonergic thalamocortical terminals (Lebrand et al., 1996, 1998). However, thalamocortical innervation is essentially postnatal, whereas newborn pups were not treated with 5-MT, and Shemer et al. (1991) have shown that after 5-MT injections, 5-HT uptake starts to normalize immediately after birth.
It is intriguing to consider that the arrival of serotonergic afferents in the cortex may alter reelin levels early enough to have a major impact on the formation of functional cortical columns, because: (1) the formation of cortical columns continues into early postnatal life and is controlled by transient cortical networks dependent on CR cells (Luhmann et al., 2003) and reelin secretion (Nishikawa et al., 2002); and (2) the development of cortical columns depends on GABAergic interneurons (Buxhoeveden et al., 2000b; Casanova et al., 2002c) that establish their connections with projection neurons at the late stages of corticogenesis and that may be at least partially guided by CR cells (Shinozaki et al., 2002; Ang et al., 2003). The columnar organization may be particularly vulnerable in the large and convoluted primate forebrain, where it is much more prominent during migratory phases of cortical development (Sidman and Rakic, 1973; Rakic, 1988, 2003).
Considering the fact that the correct allocation of neurons within the cortical laminae and columns is essential for the normal cortical columnar organization and subsequent normal brain function (Rakic, 1988), this study implicates a possible common pathway how inborn serotonergic disturbances may affect brain development. Functional failures at several points of this pathway (i.e., a mutation of the serotonin transporter gene, failures of serotonergic transmission at CR cells, a mutation of the reeler gene) may result in similar disruption of the normal cortical organization, which would support the "common neurochemical mechanistic failure" hypothesis (Chugani, 2002). Moreover, several recent studies indicated that various debilitating neurological disorders that have been linked to the serotonergic system, such as schizophrenia and autism, may be at least partially caused by developmental abnormalities of cortical columns (Selemon and Goldman-Rakic, 1999; Buxhoeveden et al., 2000a; Casanova et al., 2002a,b,c; Casanova, 2003). Our results show that embryonic serotonergic disturbances may be important for the development of some of these cortical abnormalities and, therefore, may offer new insights into the etiology of these disorders.
This work was supported by a National Alliance for Autism Research postdoctoral fellowship (S.J.) and the United States Public Health Service (P.R.). We thank Klara Szigeti for technical assistance with electron microscopy and Drs. Bridget Berechid and Kresimir Letinic for advice with Western blots. We thank Dr. Constantinos D. Paspalas for his suggestions and comments on this manuscript.
Correspondence should be addressed to Dr. Pasko Rakic, Yale University School of Medicine, Department of Neurobiology, P.O. Box 208001, New Haven, CT 06520-8001. E-mail: pasko.rakic{at}yale.edu.
Copyright © 2004 Society for Neuroscience 0270-6474/04/241652-08$15.00/0
↵* S.J. and V.G. contributed equally to this work.
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You are going to email the following Early Serotonergic Projections to Cajal-Retzius Cells: Relevance for Cortical Development
Skirmantas Janušonis, Vicko Gluncic, Pasko Rakic
Journal of Neuroscience 18 February 2004, 24 (7) 1652-1659; DOI: 10.1523/JNEUROSCI.4651-03.2004
Existence of Functional Connectome Fingerprint during Infancy and Its Stability over Months
Gli1 Regulates the Postnatal Acquisition of Peripheral Nerve Architecture
Loss of RNA-Binding Protein HuR Leads to Defective Ependymal Cells and Hydrocephalus
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SNapchat: Making an impression before IPO.
Since September of 2011, CEO and Co-founder Evan Spiegel has been a pioneer in the world of social media with the creation of Snapchat, a social platform in which users can share expiring photos and videos with each other. The idea has helped individuals around the world encapsulate what their lives are like, creating a platform that brings the world closer together. I remember when I first downloaded the app on my phone. I wasn't very interested in the functionality, but I knew that the concept was going to be big in the years to come.
Today, the company is finally ready to be placed on the stock market and given an IPO (initial public offering), which could be a great investment given the leadership in the company to push the limits of Snapchat and evolve. They have also just released a pair of glasses with a built in camera, allowing you to film and take photos from a "GoPro-like" perspective. This is probably a stunt to encourage new investors before they IPO, but a smart one if I must say.
Snapchat definitely has the name and reputation needed to compete with Facebook, Instagram, Twitter, and many other social media and camera companies. More importantly, I think there's a cool lesson to learn here, which is why I decided to discuss Snapchat in particular. The project that would become Snapchat was started by Evan Spiegel and others at Stanford University. It was a student startup in college that became the behemoth it is today. It just serves as another example that young entrepreneurs can become very successful if they pursue their ideas and ambitions. You never know, maybe your idea will become the next Snapchat.
The College Bubble continues to grow.
Check out the video above by crushthestreet.com regarding the student debt bubble.


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Q: Why objects in flask-sqlalchemy still show up as valid? After setting up two tables with the following code
class User(db.Model):
    id = db.Column(UUID(as_uuid=True), primary_key=True, default=uuid.uuid4)
    email = db.Column(db.String(100), unique=True)
    password = db.Column(Password)
    name = db.Column(db.String(1000))
    studies = db.relationship('Study', backref='owner', lazy=True, cascade='save-update, merge, delete')

class Study(db.Model):
    id = db.Column(UUID(as_uuid=True), primary_key=True, default=uuid.uuid4)
    owner_id = db.Column(UUID(as_uuid=True), db.ForeignKey('user.id'), nullable=False)

I am testing that the removal of studies happens when the user gets removed. So far, seems to be the case, but I need to get to the database to discover that:
def test_studies_removed_with_user(app):
    user = User.query.filter_by(email=app.config['TESTUSER_EMAIL']).first()
    study = Study(owner=user)

    db.session.add(study)
    db.session.commit()

    assert study

    db.session.delete(user)
    db.session.commit()

    assert User.query.filter_by(email=app.config['TESTUSER_EMAIL']).first() is None
    assert not Study.query.filter_by(owner_id=user.id).all()

if I break with the debugger in the prior to last assert, user variable is still populated, and so is study. I'd have expected these variables to be invalid now (because the asserts succeed, even the second which uses id from 'user', so the entries are removed on the database).
Is this expected behavior, or am I doing something wrong?

redpajama_set_name: RedPajamaStackExchange


Sears Holdings (NASDAQ:SHLD) is teaming up with Sonic Solutions (NASDAQ:SNIC) to enable Sears and Kmart customers using its movie download service, Alphaline Entertainment, to view flicks instantly on various mobile devices.
Sonic's multi-year deal with Sears extends the reach of the platform, known as RoxioNow, and will enable the services to be embedded at a chip level on devices such as portable media players and mobile phones. Users will be able to download movies the same day they are released on DVD.
"Collaborating with Sonic provides a great opportunity for Sears and Kmart to launch digital services for customers seeking even faster access to the latest in home entertainment experiences," said Karen Austin, president of consumer electronics for Sears and Kmart.
Sears is the new kid on the block in the online video streaming industry that is already dominated by giants like Netflix (NASDAQ:NFLX), Google (NASDAQ:GOOG) and Amazon (NASDAQ:AMZN).
The Hoffman Estate, Ill-based broadline retailer said it will continue to increase the system's reach and flexibility by providing on-demand access.


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SOMALINET NEWS - AFRICA
NEWS - FRONT
_______________________________________________Expect exciting developments.
Egypt: Security 'mistakenly' kills 12 including Mexican tourists
Libya: 40 migrants dead, over 300 rescued in Mediterranean Sea
Chad: Government forces kill 7 Boko Haram militants
Central African Republic: Returning Muslims "forced to abandon their religion,"-Amnesty
Zimbabwe: Authorities push to extradite American killer of Cecil Lion
Cameroon: 2000 Nigerians Sent Home In Fight against Boko Haram
Nigeria: 5 killed in suicide bomb attack in Friday market in northeastern Nigeria
Nigerian Liquefied Natural Gas (NLNG) Dividends Grabbed To Offset Loans
Nigeria: 71 Children freed from Boko Haram Militants
Boko Haram rebels brutally kill 10 fishermen in northeastern Nigeria
Nigeria: 'We do not know if the Chibok girls can be rescued' -says President elect
CLICK HERE FOR LATEST NEWS
SomaNet News Archive
Published on Tue, 04/14/2015 - 17:33
(By Estera Popowska)
As Nigeria marked the first anniversary of the abduction of 219 school girls by Boko Haram rebels, Nigeria's president-elect Muhammadu Buhari on Tuesday said he could not make promises on their return, AFP reported.
Nigeria's newly elected leader said there was a need for "honesty" in his new government's approach to the girls' abduction, with nothing seen or heard from the students since last May when they appeared in a Boko Haram video.
"We do not know if the Chibok girls can be rescued. Their whereabouts remain unknown. As much as I wish to, I cannot promise that we can find them," he said in a statement. "But I say to every parent, family member and friend of the children that my government will do everything in its power to bring them home," Mr. Buhari reportedly said.
AFP says a march was held in Abuja symbolically involving 219 schoolgirls, part of a number of events around the world to mark the abduction, which Amnesty International said was one of 38 since the start of last year that had seen at least 2,000 women taken by the militants.
Meanwhile, The UN and African rights groups also called for an end to the targeting of boys and girls in the conflict, which has left at least 15,000 dead and some 1.5 million people homeless, 800,000 of them children (Source: AFP)
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