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The Latest Disney's Movies, "Raya and the Last Dragon" Go Online at Gomovies
The newest addition to Disney's animated film library, which you can watch on Gomovies online, has arrived and delivers an exciting, heartwarming story with a message that feels almost too pertinent to today's world. "Raya and the Last Dragon" is set in the imaginary kingdom of Kumandra, where the humans lived in peace with mystical dragons thousands of years ago. When the Druun, a malevolent entity bent on turning everyone it comes into contact with into stone, the dragons gathered together to create a jewel Sisu, the last dragon (voiced by Awkwafina), to defeat the enemy.
People dispersed into their own parts of the planet and lived in isolation. The jewel shatters in a botched peace offering, and the Druun comes to wreak devastation. Raya (Kelly Marie Tran) will arise to save the planet from destruction.
On a great and small scale, the issue of trust, or rather the lack thereof, runs throughout the film. Raya has a hard time trusting the people she meets at times. Benja, her father, tries to teach her the value of trust and cooperation at every opportunity. Raya even employs those lessons when attempting to make friends with Namaari, a fellow princess (voiced by Gemma Chan). Namaari betrays her, igniting a vicious conflict that lasts the whole of the film that you can find on 123Movies site.
On a grander scale, Raya's father, Benja (voiced by Daniel Dae Kim), wishes to accept the other kingdoms' uniqueness in order to bring them all together as one. At first, neither party can trust the other, and they let their greed get the best of them. There are a lot of real-world analogies here. Through solid plot beats and character moments, the writing team smoothly conveys the point. They deserve kudos.
Raya is played by Tran, who heads the mostly Asian cast. She has a fierceness about her, yet she also has a charming aspect about her. Raya has an edge to her after a devastating betrayal as a child, and it's understandable. Together with Awkwafina's ever-optimistic Sisu, the two create a friendship that provides the film with both humor and emotional weight.
A delightful ensemble of supporting characters joins the action in the flick. Raya meets a variety of colorful misfits in the countries she visits, from the young, soft boat captain Boun (voiced by Izaac Wang) to the "raw and gritty" warrior Tong (voiced by Benedict Wong) to even a con artist infant and her trio of mischievous monkeys. She is first hesitant to trust them, but over the course of the film, she learns to embrace their differences and establish a "found family" with the help of Sisu. They bond via their shared Druun pain, which resulted in some powerful emotional scenes that had me teary-eyed on several times.
Raya and Namaari's interaction was the one that drew my curiosity the most. After what happened in their youth, there is a lot of tension between the two of them. The battling banter between these two is enthralling and entertaining, yet the moments they spend apart demonstrate the baggage that came with Namaari's betrayal. Despite the fact that Namaari is the villain, the audience can understand her point of view. They appear to be fully-rounded characters, with arcs that fit in nicely with the broader concept of trust.
While the picture lacked the typical Disney song sequence, it was jam-packed with thrilling action. The combat choreography was exciting and never lacked in intensity. Kumandra is a bright and beautiful land. The filmmakers were inspired by Southeast Asian countries and used aspects from many different civilizations to create a distinct environment. Kumandra stands apart from other Disney settings thanks to its inventiveness, superb animation, and vibrant colors.
"Raya and the Last Dragon" is available as part of Disney+'s premiere access, but you can also watch it on Gomovies. The price is certainly worth it if you have children to entertain or simply want a movie with a positive message.
banana	Movie	0 2 min read
Fantastic 'Sang-Chi: The Legend of The Ten Rings' on Streaming with 123Movies
At several country, cinemas have just reopened, and Shang-Chi and the Legend of the Ten Rings is the first major film to lack a crystal clear 4K version that can be easily downloaded from The Pirate Bay, despite being postponed almost a month from its US premiere, or streaming on 123Movies. This means it'll most likely be the film that reintroduces Malaysians to the multiplexes. It also means that the film has already been discussed and written about ad nauseam over the last month.
You've probably certainly heard all of the praise and clichés unless you've been living under a rock. It is, without a doubt, a victory for Asian American representation. No, Marvel did not fail to deliver when it came to staging the film's several spectacular kung-fu set pieces. (Director Destin Daniel Cretton achieves the delicate balance of paying respect while not appearing derivative.) Simu Liu gets just enough screen time to impress.
Shang-Chi and the Legend of the Ten Rings is everything that a Marvel film should be. We knew the first installment of this new "phase" of the Marvel Cinematic Universe would have to do a lot of heavy lifting, introducing new characters (Shang-Chi, Eternals) and recasting existing ones (WandaVision, The Falcon and the Winter Soldier, Loki). And this film is cut from the same cloth as the rest of the MCU's first installments. It's made to look like it came off the production line. It's fast-paced and hilarious. It has everything of the big-screen superhero antics you'd expect, plus just enough heart and soul to keep things interesting.
Shang-Chi is a joyous return to theaters and a terrific day at the movies. Tony Leung and Michelle Yeoh are two of the most influential Asian legend artists in the film.
Make no doubt about it. Tony Leung directed this film. He's in charge of every scene he's in. The Marvel movies, according to critics, have a villain problem. And, while they're always agree with that assessment, some people confident that this depiction would put any such criticism to rest.
The most important figure on screen is Leung, who plays estranged Shang-Chi's father Wenwu. He takes on entire civilizations by himself, finds love, has children, and raises a family, and does it all with the same stoicism that has made him one of Hong Kong's greatest actors of all time. His depiction is so passionate that you'll find yourself sympathizing with Wenwu's obsessive and despotic ambition.
You could believe this is just hyperbole. A fanboy's delight at the news that Tony Leung has finally (finally!) been discovered by Hollywood. But tell me this isn't the best performance you've seen in the MCU to date as you walk out of the theater.
Between Star Trek: Discovery, Crazy Rich Asians, and a slew of minor appearances in films like Last Christmas, Boss Level, and Gunpowder Milkshake, Michelle Yeoh has had something of a rebirth in recent years. She offers a seriousness to whatever role she does, whether dramatic, funny, or sassy (or all three at once), that elevates each of those performances in some way.
Michelle Yeoh may not get as much time on screen as the rest of the Shang-Chi cast, only appearing near the conclusion of the second act, but the way she behaves herself has such a calm grandeur that you'll be blown back in your seat. Despite the fact that her character's role in the film is solely explanatory, Yeoh owns every word and commands every moment, finding the ideal blend of lovely and tranquil with unyielding strength and absolute badassery.
Both of these acts are at the heart of Shang-strength. Chi's These two film legends, who have spent their careers wowing us in countless dramas, romances, and action thrillers, have finally been granted the chance to apply their magic to a Marvel film. Without the two of them, you couldn't make an MCU version of a wuxia fictional Chinese martial arts film.
When you got Shang-Chi on 123Movies streaming, you may watch it on the biggest screen imaginable. You'll come for the big-budget action and witty banter that has become a Marvel standard, but you'll stay for Tony Leung and Michelle Yeoh's sheer, unadulterated cool.
Find the Excitement of 'Eternals' Movie Online on Gomovies
Did you still remember about the world as we know it has come to an end last year on Gomovies? Right, when Thanos destroyed humanity by snapping his fingers, leaving the door open for future superheroes and parallel universes to decide how to preserve whatever else is left on the blue world. The fight in Eternals isn't so much about resurrecting life on Earth as it is about protecting it from the wicked powers known as Deviants. Unlike The Avengers and what they started out to do, the Eternals' 10-member team has only one goal: to defend humanity and guide its progress. The plot, however, is not as straightforward as it appears on paper.
Salma Hayek stars as Ajak, the spiritual leader of the Eternals, and the film features the largest ensemble cast assembled post-Avengers. She is the all-seeing Oracle who leads and protects her fellow superheroes from harm. They do, however, stray.
Thena, played by Angelina Jolie, is the most complex and evil of the Eternals. What makes her intriguing is that she is fighting her own psyche rather than the terrible Deviants. Ikaris, the group's Alpha, is played by Richard Madden, while Sprite, a woman trapped in a girl's body, is played by Lia McHugh. Kingo (Kumail Nanjiani), Gilgamesh (Don Lee), Phastos (Brian Tyree Henry), Makkari (Lauren Ridloff), Druig (Barry Keoghan), and Sersi (Gemma Chan) are the other Eternals, who add comedic relief, love plots, and variety to this Marvel adventure that also live at 123Movies Gomovies.
To beginning with, Eternals has a runtime of over two and a half hours, coming in second only to Avengers: Endgame's nearly three-hour duration. The problem isn't so much the length as it is director Chloe Zhao's attempt to cram as many background stories and subplots as possible into the film in order to give the A-list star ensemble their due. As a result, certain characters get more time on screen than others, despite their lack of charisma. Sersi and Ikaris are seen brooding about their failed love, while Makkari and Phastos are given insufficient screen time to tell us about their prior lives.
Isn't Eternals as good as The Avengers?
Eternals is visually amazing. There is imagery in every frame, and the action scenes, particularly the beginning montage, are beautifully orchestrated. However, the picture falls short when it comes to developing interesting characters and storylines that you can get invested in.
Chloe Zhao's attempt to check the boxes of diversity and inclusion seems a little forced. Yes, we support a homosexual superhero, and it's about time LGBTQ inclusion in an MCU film feels natural, but Phastos' love story with his partner is underutilized.
Eternals tries to be something but falls short of being nothing. The similarities to the Avengers only add to the misery. There are, however, a few passing nods to the Avengers, and you can't help but smile whenever you hear a familiar name.
If Eternals had spent as much time in the writing room as it did in the VFX studio, it could have worked. Eternals isn't the Avengers for Marvel fans, but if you don't go in with too high expectations, you might have a good time. The Eternals have a superhero army, but none of them are worth cheering for. Perhaps next time? You must stay tune at Gomovies to know the update.
Enjoy Favourite Movies Online at 123Movies Streaming From Home
Streaming activities or watching entertainment from home such as 123Movies is also beneficial for boosting immunity because it can reduce stress. As we all know, the COVID-19 pandemic has forced many activities to be done at home. Study, work, shop, all done from home. Well, if you want to try to reduce stress by staying safe at home, there are several streaming sites that can be accessed for free or paid.
5 Popular Streaming Service Providers in Asia
Among the many sites and companies that provide streaming film services that are popular in the world, here are some streaming sites that you can watch at home:
YouTube becomes one of the most widely used video-streaming websites on the planet. One of the factors is that users are not charged a penny to access this platform. In fact, a number of film production houses ventured to upload their work on this platform. They can also benefit from advertisements or advertisements that are installed. Another advantage, uploaded films can potentially go viral.
Netflix is one of the most popular video streaming service providers in the world. The reason is that the content presented by Netflix is quite unique and original. Some of the famous serial films include Marriage Story, The Irishman, and Bird Box. However, viewers are required to subscribe to watch Netflix's shows on Gomovies.
3. Iflix
Iflix has been present in Asia since 2016. Apart from Indonesia, this platform service is also available in Malaysia, Thailand, and the Philippines. What makes Iflix interesting is that most of its shows can be watched for free. You can also access it via a smart phone or computer or laptop.
4. HBO Go
HBO Go is a streaming service from the premium USA cable television network, HBO. HBO Go is available in various countries. HBO's content consists of exciting shows, movies, and series. Users can watch via the mobile app and the HBO Go website.
6. Disney+ Hotstar
Disney Media and Entertainment Distribution is partnering with Indian streaming service Hotstar. Disney+ Hotstar is available in Asia with a subscription fee monthly. You can access various Disney series, movies and shows. In addition there are several films under the auspices of Disney that can be watched without advertising.
Watching Streaming Movie from Home at 123Movies
Given that the epidemic is still underway and showing no signs of abating, it is preferable to do activities such as entertaining at home. You may now view movies without needing to go to a movie theater. One can view from anywhere and at any time; even previously seen movies can be paused and started at a later time. People no longer have to worry about missing out on a movie because of technological advancements, and movie prices have decreased. The majority of them will be accessible on the most recent movie streaming sites accessible over the internet. Not only that, but these sites also offer subtitles for movies from all across the world, including HD versions. All you can watch, when you can find 123Movies for your pleasure.
Amazing Visual of 'Dune' on 123Movies Streaming Online
Dune, a movie directed by Denis Villeneuve, is out in theaters and could find on any streaming online media such as 123Movies Gomovies. The audience has high hopes for this film because it is one of the most anticipated sci-fi flicks of the year. Their hopes and aspirations were sky-high. Dune has also been hailed as the best science fiction film of the year.
Part of that assumption is correct. This film, at least in terms of visuals, provides a feast for the eyes. The action scenes, the technology used, and the happenings in the desert are the main visual highlights in this picture, despite the fact that it is set in the desert for 90% of the time.
The expanse of the desert, filled with millions of mysteries, will undoubtedly inspire the audience to use their imagination. Dune, on the other hand, reveals the desert's most dangerous threat: huge worms that consume anything that makes a sound in their frequency. The worm was enormous. He was capable of swallowing a massive pennant machine and its contents. The length, however, approaches 400 meters.
In this film, the presence of the Ornithopter, a dragonfly-like helicopter, is also entertaining. Denis did an excellent job of demonstrating how the helicopter works. When the wings are manipulated, they can close and open. This aircraft looks a lot like a dragonfly. There aren't many different planes examined in this film, which is unfortunate. Another unique technologies, even like a science fiction movie that can find in many streaming movie sites.
Many Unique Character in 'Dune' Movies
Dune is a film about Paul Atreides (Timothee Chalamet), the son of Duke Leto Atreides (Oscar Isaac), and his official concubine, Jessica (Rebecca Ferguson). Strange visions about a girl in Arrakis plague Paul on a regular basis (Zendaya). Despite being a desert, Arrakis is home to a massive spice mine. Arrakis' population is primarily Fremen, who have always been oppressed. Originally, the planet was governed by the ruthless Harkonnen family. The Emperor, on the other hand, commanded that they depart the planet. He made the Atreides family rulers of Arrakis.
With a duration of 155 minutes, this film should be able to deliver a more complete and engaging story. Denis, on the other hand, appears to be preoccupied with cinematography, which makes the tale less engaging. The story appears to be devoid of soul. But, Dune focuses too much on Paul's dreams and doesn't give enough attention to the other characters. Paul's face is almost the focal point of the film. The political tension is also under-explored, which is one of the intriguing aspects of this story. The Fremen side of things isn't well-explained.
Dune did introduce the Bene Gesserit, a female-only pseudo-religious group. They are made up of spies, nuns, scientists, and theologians who utilize genetic experimentation, political meddling, and religious tinkering to further their own agenda towards the ascension of the human race with the presence of Kwisatz Haderach, their chosen one. However, the portrayal resembles that of nuns in a horror film. Duke Leto, on the other hand, had no idea what was driving the emperor's intention to send him to Arrakis. Despite this, Duke Leto made every effort to fulfill his responsibilities. He also attempted to embrace Fremen, an Arrakis native. His efforts were, however, in vain.
Dune will most likely satisfy that yearning as a visual adventure movie. Dune, on the other hand, will feel flat and dull to story plot hunters. Especially for people who have already finished reading the book.
The Outsider, HBO's Thriller Mystery Series Watch on 123Movies
HBO's presents "The Outsider", a narrative about faith at its foundation now can watch on 123Movies. The 2018 novel by Stephen King centers on a small-town detective whose profession compels him to find tangible answers to real-world riddles, and then delivers him an impossible case that he can't explain without considering otherworldly possibilities.
King asks his readers to do the same thing, to believe in the unbelievable, but the work falls well short of satisfying its own request. Perhaps the Master of Horror has spent so much time writing about ghosts and demons that he no longer feels the need to persuade anyone that believing in the boogeyman is a rational, rational choice, but the book tries to merge two diametrically opposed genres — based on speculation fiction and true crime — without honoring the foundations of both.
HBO's "The Outsider" strikes a better, if not ideal, balance. HBO's TV show 10-episode series adapted by Richard Price, who is known for writing realistic police dramas , but its author knows "The Outsider" isn't on the level of Price's previous work, but it's considerably better than this story has any right to be, thanks to a brilliant cast (headed by Ben Mendelsohn) and eerie, austere direction from Emmy-winner Jason Bateman.
A Mysterious Case of Murder
Meet Ralph Anderson (Mendelsohn), a cop whose work requires him to deliver both good and bad news. The drawbacks are as follows: Not long after the death of his own teenage son, the Georgia-based detective came upon a particularly heinous case involving a viciously killed child. Although Ralph's lone child was not killed or sexually assaulted in the woods, the still-recovering cop isn't over the tragedy of losing his child. What's the good news? He knows who is to responsible for the latest death, and he intends to hold him accountable.
That seems like an intriguing opening to a murder mystery, and it is, even if "The Outsider" isn't a murder mystery at all. "Don't worry, there will be an explanation for all this craziness," the story promises right away. And a lot of period in the early episodes is given to anchoring this dark and brooding drama; jokes about director Bateman's typical low-lighting are already being made, but it's an asset, not a drawback. I never struggled to understand what was going on in "The Outsider." The strong lines between the dark and bright areas of the screen, on the other hand, represent the competing values at play. What evil lies in the shadows might be mysterious, but so can what is visible in the light.
Erivo's private investigator, on the other hand, represents the flip half of the coin. Holly is a trained dreamer, but Ralph is a firm believer in reality. She's witnessed things she can't explain and has come to terms with the fact that such unsolvable puzzles are a part of life. When she comes, "The Outsider" shows its true self: that this case isn't going to be solved by typical police work; that the answers to the impossible questions aren't tied to a devious killer's ingenious plot or a hint missed by the intrepid detectives. In a nutshell, the solution is magic.
It's not a Magic?
It's possible that "magic" is the wrong choice of words for an otherworldly explanation, but understanding that "The Outsider" isn't a true crime story (or, more accurately, it's not a fictionalized crime story that represents reality) is crucial to enjoying the show — to the point where anyone expecting a realistic conclusion might be offended by the supernatural twist. Most people are probably aware of Stephen King's work because of his trademark, but the author doesn't always rely on supernatural events to explain things; Andy Dufresne didn't dig his way out of Shawshank. Price's show performs a better job at teasing its hand than the novel, owing to the fact that it progresses through the inquiry considerably faster. However, the series could still be doomed by the book's biggest blunder. "
The Outsider", that can enjoy online at Gomovies must be a superb drama because it isn't actually a murder mystery — at least, it doesn't work as one. Good dramas include likable characters (check), life-changing situations (check), and frequently tackle relatable subjects brought up by these people and events. The subject at hand is faith. What impact does our faith have on our lives? Can we be protected from anything if we don't believe in it? Is it possible that having too strong a belief can harm us? Price poses these ideas through situations and sentences not seen in King's novel, encouraging his audience to consider more than "Did Terry Maitland kill that little boy?" There's a new road to what could be a new finale after the six episodes presented for review. HBO's "The Outsider" is already more rewarding, even if it returns to the novel. That doesn't make it great television, but it offers you cause to believe it aspires to be.
banana	TV Show	0 3 min read
WandaVision: Sad Story of Wanda Maximoff to Vision Watch Online at 123Movies
Watching WandaVision on 123Movies is one of the most interesting activities in this mid season. Kevin Feige, the brains behind Marvel Studios, unveiled numerous superhero series titles that will air on the Disney+ streaming channel at the San Diego Comic-Con event in August 2019. Surprisingly, the storyline of the series is immediately interwoven into the most recent phase of the Marvel Cinematic Universe brand.
WANDAVISION is the title of the first series as well as the start of the fourth phase. It certainly raises a lot of questions regarding the tale based on the title. In the film AVENGERS: INFINITY WAR, Vision's character is believed to have perished after Thanos removed the mind stone from his head (2018).
1. Sitcom Love Letters in the Golden Age
The first two episodes of WANDAVISION TV Shows are mind-blowing. Wanda (Elizabeth Olsen) and Vision (Paul Bettany) are reported to be married and live in the small hamlet of Westview. In general, they strive to live an ideal existence as a pair. Wanda is preoccupied with daily chores at home, including mingling with neighbors, while Vision works in an office.
The pilot episode is designed to look like a 1950s comedy, complete with (fake) commercials, as well as succeeding episodes that represent each decade. The concept was supposedly intended to be a love letter to America's golden era of comedies, from black-and-white to color, from decade to decade.
The first two episodes of the series, directed by Matt Shakman, were entertaining to watch and left the viewers wondering where the plot would lead them. The fact that Wanda and Vision exist in an other reality is revealed only after the third episode reaches its conclusion. In the real world, the planet is improving as a result of Thanos's finger snap, which is dubbed The Blip.
2. A Vision of Existing in a Parallel Universe
In the fourth episode, the focus shifts to Monica Rambeau (Teyonah Parris), an adult who returns after being abducted by The Blip. He is a member of the S.W.O.R.D organization tasked with investigating the disappearance of the city of Westview and its contents. Monica's body is imprisoned in a vortex while she is researching, where Wanda Maximoff lives happily with Vision and their two children, Tommy and Billy.
Monica discovers that Wanda has not been able to move on from Vision's death after being thrust back into reality. The telekinetic woman then brings her beloved back to life in an other dimension. Monica tries to aid Wanda with the help of agents Jimmy Woo (Randall Park) and Darcy Lewis (Kat Dennings). Is it true, however, that Wanda truly wants to be helped?
3. Maintain your identity while putting on a new display
After going a year without a single release from the Marvel Cinematic Universe catalog for the first time, Marvel Studios delighted fans once again in early 2021 with the highly anticipated WANDAVISION. The nine-episode series takes a risk by showing something that hasn't been seen before in the franchise's 13-year history.
Imagine if, in the absence of wind and rain, Jac Schaeffer, the inventor, integrated elements from 1950s to 2000s sitcoms. Not only was he arrogant, but he also managed to blend into the construction plot. As a result, WANDAVISION has managed to reinvent himself while maintaining his identity as a Marvel superhero. Because, aside from the comedy components, there is still a wonderfully wrapped tale (not to mention certain easter eggs that were mysteriously utilized in the next film or series) as well as thrilling action moments with eye-popping visual packages.
4. A Heartbreaking Story
Apart from the fact that WANDAVISION is part of the Marvel Cinematic Universe's fourth phase, it's crucial to note that this series is about a loss. Wanda Maximoff can't forget about Vision, who was killed by the enemy. On that front, we'll see that Wanda has a strong affection for Vision. Until you realize the cause for his desire to resurrect his sweetheart, it stings. This is a very human motive. And you can enjoy all that by watching Disney+ streaming via online on 123Movies Gomovies.
The Unanswered Questions in 'Army Of Thieves' has Revealed After Watch On 123Movies
The prequel to Army of the Dead, Army of Thieves, leaves some unanswered questions – you would find the answers after watching online at 123Movies. The tale of Army of Thieves is set several years before the events of Army of the Dead and follows Ludwig Dieter, also known as Sebastian, the safecracker who unlocks Army of the Dead's Götterdämmerung safe. Dieter is hired as the new safecracker by international crooks Gwendoline, Rolph, Korina, and Brad.
Dieter is tasked with breaking three of Hans Wagner's four legendary safes: Das Rheingold, Valkyrie, and Siegfried. All three are found and opened by the crew. Everyone save Dieter, however, winds up in Interpol's hands in the end. The origins of Army of the Dead's Ludwig Dieter are revealed in great detail in Army of Thieves. The film's conclusion, on the other hand, fails to answer some of the issues and puzzles that have been raised during the film. Here are the answers to Army of Thieves' most burning questions.
What Role Does Bly Tanaka Play In The Army Of Thieves Events?
In Army of the Dead, Bly Tanaka, a casino owner, employs Scott Ward's crew to collect $200 million from the Götterdämmerung beneath the zombie-infested Vegas Strip. Korina shows the crew a photograph of Tanaka's Army of Thieves appearance during her intel presentation on Wagner's safes. Tanaka has all four of Wagner's famed safes, which could indicate that he was part in Dieter's quest to find and open each masterpiece, as revealed in Army of Thieves. Given Tanaka's resources, it's plausible that he engineered the events in Army of Thieves, including the keyhole competition, to discover the individual capable of unlocking all four safes. Tanaka could've tried to find Dieter earlier, despite the fact that his genuine objective in Army of the Dead was to retrieve an alpha zombie's head. Dieter may be the only locksmith competent to secure Tanaka's wealth because he is the guy who most knows Hans Wagner's goal. It would also make sense if Gwendoline had been working for Tanaka all along. You can find this scene when you watching it by streaming online movie on the internet.
Is Korina a Traitor to Gwendoline and Dieter?
Korina is the expert hacker among the ensemble of international criminals in Army of Thieves. Interpol apprehends Korina after the third and last theft. Korina is told by Beatrix that if she gives up the other members of her crew, Interpol will take after Korina's younger brother while she is in prison. While Korina seemed to be considering the offer, Army of Thieves does not reveal whether she accepted it or betrayed Gwendoline and Dieter. However, the next scene shows Interpol apprehending Gwendoline and Dieter, implying that Korina betrayed them. Another scenario, which makes more sense, is that Korina handed Interpol the location of Brad and Rolph instead. While Korina is unlikely to betray Gwendoline or Dieter, she has no love for Brad and Rolph, and handing them up would be a far better deal for guaranteeing the future of her younger brother.
Why did Delacroix let Dieter to flee?
While Army of Thieves isn't a zombie film like Army of the Dead, it does feature a classic film trope: the robbery. Delacroix also plays an important role in this theft as an Interpol agent pursuing down the perpetrators. Delacroix finally gets his big break after he catches Gwendoline and Dieter trying to depart on a boat after spending the majority of the film being outsmarted. Gwendoline manages to persuade Delacroix that Dieter is a nobody who needs to be fired. On the one hand, Dieter is an amateur robber who is in well over his head, unlike the rest of the crew. Dieter, on the other side, is a master safecracker who, despite being captured, has managed to open some of the world's most difficult vaults. It's inexcusable that Interpol would let go of such a crucial suspect in such a high-profile case.
The explanation about all those questions will find after you watching it very carefully at 123Movies site or Gomovies. However, by those streaming movie online site, can help you find any movie that you want without kind of problem.
You Can Watch A Game Addicted Movie Like 'Free Guy' on Gomovies
You're familiar with all of your favorite video game's non-player characters, right? Have you ever thought what might happen if they gained free will all of a sudden? No? It doesn't matter, though, since you'll find out once you watch Free Guy on 123Movies.
Free Guy is a film directed by Shawn Levy and based on a script by Matt Lieberman and Zak Penn. It tells the narrative of an NPC who takes control of a man's life. And he might well bring down the entire virtual world he's living in in the process. Ryan Reynolds, Jodie Comer, Lil Rel Howery, Utkarsh Ambudkar, Joe Keery, and Taika Waititi feature in the sci-fi action comedy.
Welcome to Free City, an open-world video game (sounds like Sim City but plays like GTA). Guy (Reynolds) is a non-player character that works as a bank teller. Guy resolves to make himself the hero after becoming self-aware as a result of a program installed into Free City by the publisher Antoine (Waititi). With the devs hurrying to shut down a game and Guy attempting to salvage it, it's a race against time. And, as you can anticipate, everything goes to hell.
Jacksepticeye, Ninja, Pokimane, DanTDM, and LazarBeam are among the gamers and streamers who make cameo cameos in Free Guy. Alex Trebek makes a cameo appearance in the film after his death. Hugh Jackman, Dwayne Johnson, Tina Fey, and John Krasinski will all make cameo appearances in the film. Free Guy appears to be a wild and funny journey based on what we've seen so far about the film. And we're here to address all of your questions regarding where to see the movie, when it'll be available on the internet, and whether or not it'll be released in cinemas.
Is Free Guy available to watch online?
Unfortunately, the answer is no. While most major studios are releasing films simultaneously on streaming for watch online and in cinemas, Free Guy will be released solely in theaters, at least for the time being. Both Free Guy and Marvel Studios' martial-arts action film Shang-Chi and the Legend of the Ten Rings will have exclusive new movies when they premiere in May 2021, according to Disney CEO Bob Chapek. It's presently unknown whether the similar method will be used in other Disney films released in 2021.
Is Disney+ Premier Access available for Free Guy? The answer is No, most likely not. We still don't know if Free Guy will be available on Disney+ or Hulu whenever it is released for streaming, so there is no official news on this. For an extra charge of $29.99 on top of their existing Disney+ subscription, users may stream movies while they're still in theaters with the Disney+ Premier Access option. This was the case with some of Disney's most anticipated 2021 releases, such as Black Widow and Cruella. However, as Free Guy will only be available in theaters when it is released, you won't require Premier Access to view it after it becomes available on streaming Gomovies.
Is Free Guy now showing in theaters?
In the United States, Free Guy will be released in theaters on August 13, 2021. The film's original release date was nearly a year ago, so fans will be overjoyed to finally watch it on the big screen. The film was supposed to be released on July 3, 2020. However, the COVID-19 epidemic delayed the release of Free Guy, as it did most of the movies in 2020. It was first pushed out to December 11, 2020, before being removed entirely from the studio's release plan. The date was then changed to May 21, 2021.
Ryan Reynolds revealed in March 2021 that the debut of Free Guy has been rescheduled, this time to the current date. I'm hoping this one sticks. Moviegoers have been absent from cinemas for a long time, but now that the number of coronavirus infections is declining across the country and the vaccine deployment is being increased, they can finally return. However, before purchasing performance tickets, make sure you follow all safety precautions and check your state's most recent safety requirements.
Watching The Suicide Squad, 8th Ranked The International Box Office via 123movies
When looking for new released movie online via 123movies, this is one choice you can pick. The Suicide Squad is now ranked eighth internationally (according to Box Office Mojo) against other US film releases in 2021. F9, Godzilla vs. Kong, Black Widow, A Quiet Place II, Cruella, Jungle Cruise, and Space Jam 2 are the films that come before it. However, it has done exceptionally well in international markets. It is ranked sixth in the international box office, ahead of Jungle Cruise and Space Jam 2.
Films having a lot of action and/or slapstick comedy, as well as light, easy dialogue, usually do well in foreign markets. For non-English speaking audiences, less is lost in translation, making it easier for a wider audience to enjoy. The Suicide Squad is packed with action, which is a definite bonus in terms of its international reception. Nonetheless, it's fascinating to observe how well the film's humorous banter and emotional depths and subplots – particularly Ratcatcher 2's speech to Bloodsport about finding a purpose in life and her reminiscence with her father – seem to be translating with overseas audiences.
Original Concepts for Harley Quinn and Incubus from 'Suicide Squad' by David Ayer
It's no news that Warner Bros. gave director David Ayer's 2016 picture Suicide Squad some harsh trims during filming and post-production. Ayer has used Twitter on multiple occasions to clarify the difference between his original concept for the picture and what was actually produced. Ayer returned to Twitter to give his idea of the film's villain. Ayer published a piece of concept art for Incubus in a tweet with the comment "This is the look I wanted."
Incubus is noticeably slimmer in this image than he is in the film that can watch on gomovies. While the look is still flashier than its comic book origin, it leans more toward an eldritch creature than a demon aesthetic. The armor is bigger and white in appearance, giving it a skeletal aspect. The shimmering headgear that sits between the horns in the film Incubus is conspicuously absent from Ayer's version.
Ayer also shared a number of concept images for Harley Quinn's (Margot Robbie) appearance. Her red, blue, and white color scheme was used in numerous combinations, with some including the black from her comic book drawings. "We tried out a lot of various ideas and concepts." In a tweet, the director stated. "A director will be shown various iterations, often from multiple artists. It's a long procedure that necessitates the efforts of a large number of creative collaborators." He showed images of the character's test coats that he had sewn.
The Task Force X squad is made up of numerous bad guys and misfits that are ordered to hunt down Enchantress (Cara Delevingne) and her brother Incubus in the film (Alain Chanoine). The demonic siblings have the capacity to transform humans into beasts in order to destroy humanity. The film's version of Incubus is more flashy than his comic strip design. With a firey material behind black metal armor, he takes on a more demon-like look.
With the release of Zach Snyder's Justice League, a new movement called #ReleaseTheAyerCut has gained traction, with fans hoping to view Suicide Squad without the studio's cuts in the future. As the DCEU gains confidence in their productions and gives filmmakers more creative flexibility, it may be a fascinating move to re-release older films with their original cuts to fans. Below are some of Ayer's original ideas. The Suicide Squad eventually surpassed the $100 million milestone at the international box office over the weekend. It was already close at the start of the weekend, but the extra $4.6 million it made by screening in 6,530 theaters in 73 countries was just enough to push it over the top. It has now generated a total of $101.8 million dollars outside of the United States, bringing the total to $154.6 million globally.
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The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
Adriana Granzotto1,
Fabrício R Lopes1,
Emmanuelle Lerat2,3,
Cristina Vieira2,3 &
Claudia MA Carareto1
Several studies have shown that genomes contain a mixture of transposable elements, some of which are still active and others ancient relics that have degenerated. This is true for the non-LTR retrotransposon Helena, of which only degenerate sequences have been shown to be present in some species (Drosophila melanogaster), whereas putatively active sequences are present in others (D. simulans). Combining experimental and population analyses with the sequence analysis of the 12 Drosophila genomes, we have investigated the evolution of Helena, and propose a possible scenario for the evolution of this element.
We show that six species of Drosophila have the Helena transposable element at different stages of its evolution. The copy number is highly variable among these species, but most of them are truncated at the 5' ends and also harbor several internal deletions and insertions suggesting that they are inactive in all species, except in D. mojavensis in which quantitative RT-PCR experiments have identified a putative active copy.
Our data suggest that Helena was present in the common ancestor of the Drosophila genus, which has been vertically transmitted to the derived lineages, but that it has been lost in some of them. The wide variation in copy number and sequence degeneration in the different species suggest that the evolutionary dynamics of Helena depends on the genomic environment of the host species.
Transposable elements (TEs) are ubiquitous components in prokaryotic and eukaryotic genomes. They constitute the largest part of some of them [1], and play an important role in their evolution [2]. Genome sequencing has shown that TE sequences constitute about 15% of the Drosophila melanogaster genome [3, 4], about 45% of the human genome [5], and up to 90% of the genomes of some plants [6]. Why species harbor such different proportions of TEs is still unknown, but it may be related to the reproductive characteristics and population size of the host [7, 8], and to environmental stresses [9] that may influence TE dynamics. Various different mechanisms may prevent genome invasions by TEs, ranging from DNA deletions [10, 11] to epigenetic control mechanisms, such as chromatin conformation [12].
Several studies have shown that genomes harbor a mixture of TEs, some of which are still active, whereas others are ancient relics that have degenerated [13–16]. Degraded elements can result from point mutations or from DNA deletion [17]. Deletions may occur either by recombination, which is common to all classes of elements, or as a consequence of the transposition mechanism of the specific element concerned. For instance, the latter occurs in LINE elements (Long Interspersed Nuclear Elements, also known as non-LTR retrotransposons or retroposons), which are retroelements that use an RNA intermediate to transpose [2]. Their transposition mechanism leads to 5' end truncations of the new inserted sequence. Considerable internal deletions have previously been reported in the inactive copies of several non-LTR retrotransposons [10], and this deletion mechanism seems to act at an high rate, since sequences that are similar at the nucleotide level may have very different internal deletions [10, 11, 18, 19].
One example of this type of TE evolution is the retrotransposon Helena, which is a 4,912 bp LINE [18] first reported in the D. virilis species [20], and later identified in all the species analyzed in the melanogaster subgroup, as well as in D. pseudoobscura [10]. Helena has been shown to be present at different stages of its life-cycle in natural populations of D. melanogaster and D. simulans. Only degenerate copies were found in D. melanogaster [4, 18, 19, 21, 22], whereas in D. simulans several different types of sequences have been identified, ranging from highly degenerate to putatively active ones [18]. The analysis of Helena in these two closely-related species has shown how important the host genome can be in the evolution of a TE, and how important it is to analyze specific TE families in a wide spectrum of species. This is possible now that the genome sequences of 12 Drosophila species [23–26] are available, and Helena can be considered to provide an ideal model system for investigating TE evolution across a range of species.
We investigated the evolution of Helena sequences using a combination of experimental and population analyses with sequence analyses of the 12 Drosophila genomes, and we propose a possible scenario for the evolution of the element in the different host genomic environments that influence the "fate" of TEs.
Identification and analysis of reference copies
Using the full-length copy of Helena already identified in the draft sequence of the D. simulans genome [18], and 23 reverse transcriptase (RTase) fragments of the melanogaster species group [10], we performed a search for Helena-like elements in the other 10 Drosophila genomes. We identified Helena reference sequences in all the genomes (Additional file 1), apart from D. pseudoobscura, D. persimilis, D. willistoni and D. grimshawi (Figure 1). The results of Blast analyses in D. pseudoobscura, D. persimilis and D. willistoni revealed only short sequences (10 copies ≤ 210 bp, 23 copies ≤ 251 bp and seven copies ≤ 100 bp, respectively) with percentage identities to the BS element that were higher than or similar to those to Helena (D. pseudoobscura – BS: 78%, Helena: 69%, D. persimilis – BS: 80%, Helena: 74%, D. willistoni: BS: 85%, Helena: 89%). BS is a previously-described LINE element that belongs to the same "jockey clade" as Helena [27]. Sequences equally distant from BS and Helena have also been reported in D. melanogaster, and it has suggested that they may constitute a new family, named Helena/BS [21]. We did not therefore classify these sequences as Helena elements, and performed no further analysis of them. Our results for D. pseudoobscura are not consistent with previous data reported by Petrov et al. [10], who isolated just one sequence in this species. However, we should note that the same strains were not used in these two studies, and this could explain the differing results. We did not detect any Helena sequences in D. grimshawi, which is consistent with the fact that this a TE-poor genome [25], but in all the other species the selected reference copies we examined all contained one or two open reading frames (ORF1 and ORF2) (Figure 2).
Phylogenetic tree of the Drosophila genus. Phylogenetic relationships between the Drosophila species of which the genomes have been sequenced (modified from http://rana.lbl.gov/drosophila). Presence (+) and absence (-) of Helena.
Helena structure in Drosophila species. Structure of the reference copies of Helena-like elements. DNA sequences: AAA, poly-A tail. Protein sequences: gag, nucleocapsid-like domain; RTase, reverse transcriptase; *: position of start codons; #: position of stop codons. D. simulans and D. melanogaster structures are from Rebollo et al. [18]. The sequences are in Additional file 1.
The D. sechellia reference sequence (see Additional File 2 for details) is 4,888 bp long, and contains several of the hallmarks of Helena-related elements that have already been characterized: a 15 bp poly-A tail, and two overlapping open reading frames (ORF1 and ORF2). The first ORF is 1,732 bp, and the second 2,870 bp long. Both ORFs are interrupted by three premature stop codons, and the pol-like protein contains only the reverse transcriptase domain.
In the other species in the melanogaster group, the reference copies are smaller than the Helena reference copy in D. simulans. In D. erecta (see Additional File 3 for details) and D. ananassae (Additional File 4), the ORF1 and ORF2 are separated by sequences of 279 bp and 401 bp respectively, but both species contain the apyrimidic endonuclease, the exonuclease and the reverse transcriptase domains (Figure 2). In D. yakuba (Additional File 5) and D. virilis (Additional File 6) the reference copies consist only of a small ORF2, 1,755 bp and 561 bp in length, respectively.
The reference copy of D. mojavensis is the only putatively active Helena-like element identified in the species analyzed (Additional File 7). It is 4,502 bp long, and harbors two overlapping ORFs without premature stop codons. The first ORF is 1,782 bp long, and potentially encodes a 593 aa protein. The second is 2,722 bp long, and could encode a 907 aa protein. Moreover, the gag-like protein contains a conserved PRE_C2HC domain, and the pol-like protein contains the three domains required for its function: an apyrimidic endonuclease, an exonuclease and a reverse transcriptase domain. However, the poly-A tail was not identified.
Genomic Sequence Analyses
The Helena copy number for each species is presented in Table 1. This number varies considerably between the species, ranging from seven in D. erecta to 181 in D. sechellia, with sizes ranging from 80 bp (D. yakuba) to 4,888 bp (D. sechellia). The average percentage identity of the copies with the reference within each species ranged from 91.51% (D. ananassae) to 95.95% (D. yakuba). Size-divergence between these copies results from the presence of indels that occur throughout the sequences (Additional File 8). No relationship can be inferred between the size and the percentage identity, since copies with small sizes display an identity level as high as the larger ones (Figure 3). This may be expected when considering only the 5' truncations, produced by the transposition mechanism of Helena. However, the different copies reported here are also internally deleted, and can be expected to be old relics of Helena, with more neutral mutations than the full-length copies. The only exception concerns D. sechellia, in which significant correlation between size of the copies and identity was detected. This is probably due to the large number of copies detected in this species.
Table 1 Characterization of the Helena elements found in Drosophila genomes.
Helena copies in different genomes. Distribution of the lengths (intervals: 350 bp) and percentage identity of the Helena copies in D. simulans, D. melanogaster, D. sechellia, D. yakuba, D. erecta, D. ananassae, D. mojavensis and D. virilis.
Table 2 shows the average GC content for the upstream and downstream regions of the Helena insertions for each species. It ranges from 36.8% (D. erecta) to 43.1% (D. melanogaster). These values are closer to the intergenic values for these species (35.3% – 39.9%) than to those for the gene regions (46.8% – 50.4%), suggesting that Helena is more abundant in the non-transcribed genomic environment [4, 16, 28–30]. The genome of D. melanogaster is the only one in which the GC values were intermediate between those of the intergenic and gene regions. This could simply reflect the better annotation available for the D. melanogaster genome.
Table 2 Average GC content calculated in the first 5,000 nt flanking regions of the Helena copies, and of the intergenic and gene (exon plus introns) regions from Drosophila genomes
Helena-related sequences form a monophyletic clade (Figure 4) that includes a well-defined cluster of the species of the melanogaster subgroup (D. yakuba, D. simulans, D. melanogaster, D. erecta and D. sechellia). The other group contained D. ananassae, D. virilis and D. mojavensis. This phylogeny is typical for a TE with vertical transmission.
Phylogenetic tree of RT proteins. The reconstruction was performed by the maximum likelihood method with the LG model, using the Helena reference copies obtained in this study and other LINE elements based on their partial reverse transcriptase domains. The sequences used were obtained from GenBank, and are identified by the TE name and the host names (* Sequences obtained from GenBank; 1Sequences obtained in this study; 2Helena sequences obtained by Rebollo et al. [18)]. The numbers indicate the branch support calculated by bootstrap analysis consisting of 100 replicates. Only bootstrap values greater than 50% are indicated.
Analysis of the activity in natural populations
Since our in-silico analyses show that only D. simulans and D. mojavensis harbor full-length, putatively active copies, we performed Southern blot analyses on several populations of these two species to infer their TE activity (Figure 5 and Additional File 9). Our results show that these species display insertion polymorphism, suggesting that Helena is active. We cannot of course exclude the possibility of restriction fragment length polymorphism, which would lead to the same kind of result. The qRT-PCR experiments on these two species show that Helena is highly expressed in D. mojavensis, which displayed population variability that contrasted with that of D. simulans, in which the levels of expression were much lower (Figure 6). We were only able to compare the Helena transcripts, because the housekeeping gene (rp49) used to normalize qRT-PCR is equally expressed in both species (Additional File 10).
Southern blot analysis of Helena in D . simulans and D. mojavensis populations. Lanes 1 to 8 are D. simulans populations (1: North America (Tucson stock center: 14021-0251.195), 2: Junco do Serido (PB, Brazil), 3: Itaúnas (ES, Brazil), 4: Lençóis (BA, Brazil), 5: Onda Verde (SP, Brazil), 6: Ratones (SC, Brazil), 7: Seychelles (Seychelles), 8: New Caledonia (Tucson stock center: 14021-0251.216). Lanes 9–11 are D. mojavensis populations (9: Catalina Island (California, U.S.A, Tucson stock center 15081-1352.02), 10: Grand Canyon (Arizona, U.S.A), 11: Sonora (Mexico, Tucson stock center: 15081-1352.24)).
Analysis of Helena activity in natural populations. Helena transcripts (ratio Helena/rp49) of ovaries and carcasses of D. simulans and D. mojavensis (see Methods for additional information). D. simulans strains from the Drosophila species stock center: 1 (14021-0251.195 – North America, U.S.A), 2 (14021-0251.194 – Winters, California, U.S.A), 3 (14021-0251.198 – Noumea, New Caledonia), and from natural populations 4 (Amieu, France), 5 (Valence, France). D. mojavensis strains from natural populations: 1 (Grand Canyon, Arizona, U.S.A), and from the Drosophila species stock center 2 (15081-1352.02 – Catalina Island, California, U.S.A), 3 (15081-1352.09 – Santa Rosa Mountains, Arizona, U.S.A), 4 (15081-1352.24 – Sonora, Mexico), 5 (15081-1352.22 – Catalina Island, California, U.S.A). Black = ovaries. Gray = Carcasses. Standard deviation is indicated with bars.
This study describes the evolutionary dynamics of the Helena non-LTR retrotransposon in the sequenced Drosophila genomes. We have shown that Helena occurs in D. sechellia, D. yakuba, D. erecta and D. ananassae (melanogaster species group), in D. mojavensis (repleta group) and in D. virilis (virilis group), as well as in D. melanogaster and D. simulans, which had been studied previously [10, 18]. In D. pseudoobscura, D. persimilis and D. willistoni, the small copies that we found displayed similar percentage identities to Helena as to BS, a non-LTR retrotransposon related to Helena. Sequences with the same kind of similarity have been reported in D. melanogaster, and were grouped by the authors as Helena/BS family [21]. The D. pseudoobscura, D. persimilis and D. willistoni sequences we found can be included in this family. We agree with the suggestion that there could be members of the Helena/BS family in these species as shown in D. melanogaster [21], and so we did not include them in our study. The 907 bp sequence of Helena that had been previously described (GenBank AF012036) in a strain (stock center: 14011-0121-0, Tucson, Arizona) of D. pseudoobscura [10] was not found in the sequenced genome of this species. Our phylogenetic analysis shows that Helena is a monophyletic group of sequences patchily distributed in the species of the two subgenera of the genus Drosophila (Figure 1). Taking our data together with those from other authors [10, 18, 20], we can conclude that Helena was present in the common ancestor of the Drosophila genus, and has been vertically transmitted to the derived lineages, but subsequently lost in some of them, or at least diverged so much as to escape detection. The high variability in copy number and sequence degeneration in the different species shows that the evolutionary dynamics of Helena depends on the genomic environment, as has already been reported for other retrotransposons, such as Tirant [31].
Recent work has reported the presence of deteriorated and inactivated Helena in the D. melanogaster genome, but also of a full-length copy in D. simulans carrying all the structures required for activity, and with a high level of insertion polymorphism in the natural populations [18]. This suggests that in this species Helena is either still active or has been active until recently. In our analysis, all the reference copies in the other 10 Drosophila genomes were found to be devoid of intact ORFs, except in D. mojavensis. In this species, two copies of Helena contain intact ORFs, which suggest that full-length and potentially active Helena sequences could exist. This means that this species may offer a unique opportunity for studying the evolutionary dynamics of Helena.
Our analysis of the copy numbers reveals a strikingly variable distribution of Helena in the 12 species. In D. sechellia, 181 copies of Helena were observed, whereas in D. erecta only seven copies were identified. Even though these genomes have not been well annotated, and there are some low quality sequences that could bias copy number estimation, this should not invalidate the tendency identified. It has been proposed that effective population size could be one of the main factors accounting for differences in copy number of TEs [32, 33], with selection against TEs being less effective in smaller populations [34]. This seems to apply to D. sechellia. This species is restricted to the Seychelles Islands in the Indian Ocean, and is the most specialized fly within the melanogaster group. It has a very low effective population size [35, 36], and carries the highest number of Helena sequences recorded (181 copies). We would expect to find a similar scenario for D.erecta, which is also a specialized species with a small population size [37, 38]. However, as has also been observed for the mariner element, the observed copy number of Helena is not in fact consistent with this hypothesis [37]. This means that other factors may be affecting the copy number of TEs, including genomic and environmental features.
We did not observe any major differences in copy number between the two species of the Drosophila subgenus, even though D. mojavensis has a higher copy number than D. virilis. What is more striking is that the only potentially full-length active copies identified were detected in D. mojavensis, together with the other potentially active copy previously described in D. simulans [18]. The Southern blot analyses of several natural populations of D. mojavensis and D. simulans suggest that Helena has recently displayed transpositional activity. However, we confirmed that the transcriptional activity is low in D. simulans, as had previously been suggested [18], and this clearly indicates that Helena is being lost in this species. In D. mojavensis, the situation is completely different. We have observed high levels of Helena transcripts in several populations, confirming its activity. Moreover, expression varied between different populations, indicating that Helena must be present at different stages of its evolutionary cycle, i.e. different stages of activity, in different populations within this species.
Our analysis has shown that the evolution of Helena is influenced by the host species, resulting in differences in copy number, degradation and activity. In all the species analyzed, D. mojavensis is the only one in which the Helena has survived, which gives us a unique opportunity to observe the "fate" of this TE. However, it is crucial to investigate natural populations from this and other species in the repleta group in order to find out how widespread the scenario described here actually is, and to understand the process and speed of the degradation and extinction of Helena.
Here we show that six species of Drosophila have Helena transposable elements at different stages of its evolution, and may represent different stages of the TE evolutionary cycle. The copy number is highly variable in different species, but most of them are truncated at the 5' ends and display several internal deletions and insertions. In all the species analyzed, Helena has only survived in D. mojavensis, and this gives us a unique opportunity to track the "fate" of this TE.
In-silico analyses
The draft sequence data from the 10 related Drosophila Genome Sequencing Projects used in this study are listed in the Table 3. Sequence searches for the Helena element were carried out using the 4,912 bp full-length sequence of Helena characterized in D. simulans [18] as the query. Twenty-three partial sequences of reverse transcriptases (RTases) of Helena from the melanogaster group [10], and the two LINE elements closest to Helena, BS [27] and X [39] were found. Comparisons between the query and the Drosophila data set were performed using High Scoring Pairs (HSPs) within BLAST algorithms [40] with an E-value ≤ 1e-10. The sequences correspond to the three best BLAST hits, which were more than 80 bp in length, and were selected and extended to include 3 kb of the flanking regions. These sequences were analyzed using the ORF-FINDER program to identify putative occurrences of coding regions [41]. Conserved domains were predicted using the "Conserved domain search" tool from NCBI, as well as BLASTn and BLASTx searches against the nt and nr databases, respectively. Thus, we determined the most complete Helena sequence in each of the 10 Drosophila species to be considered as a reference copy within each genome. To determine the copy number in each species, the previously-determined reference copy was blasted against the full genomes. Significant matches were required to be more than 80 bp long, and to have at least 80% identity [42]. Regions similar to Helena that were separated by less than 200 bp were considered to be a single insertion. For each genome, the copies have been aligned with the reference, using MUSCLE [43] and the % identity to the reference was computed using the DNADIST program from the PHYLIP package [44]. The flanking regions of each insertion were extracted for analysis of the GC content in the first 5,000 nt using "geecee" of the EMBOSS package [45]. The GC contents of the flanking regions of the Helena elements were compared to those of the intergenic regions and genes (exon plus introns) in the other Drosophila genome versions, as noted in the Table 3.
Table 3 Genome sequences used in this study
Phylogenetic analyses
The sequences used in the evolutionary analysis were obtained from GenBank: Jockey (M22874), TART (U14101), Doc (X17551), F (M17214), BS (X77571) and X (AF237761) of D. melanogaster; Jockey of D. funebris (M38437); Amy of Bombyx mori (U07847); JuanA of Aedes aegypti (M95171); JuanC of Culens pipientis (M91082); NCR1th of Chironomus tentans (L79944); Helena of D. mauritiana (AF012043), D. simulans and D. melanogaster [18]. The multiple alignment of the RTase proteins from 11 LINEs of the Jockey clade, and the six Helena reference sequences described here (see Additional Files 1, 2, 3, 4, 5 and 6 for details) was performed using CLUSTALW [46] with the default parameters, and the alignment was manually curated using a sequence editor. The evolutionary relationships were reconstructed using the maximum likelihood method for the LG model [47] as implemented in the PhyML software [48]. The bootstrap analysis consisted of 100 replicates.
Southern blot
The occurrence of Helena in populations of D. simulans and D. mojavensis (Additional File 8) was confirmed by Southern blot using the detection system Gene Images CDP-Star detection module (Amersham Biosciences, Little Chalfont, UK). Genomic DNA was prepared from 50 adult flies [49] and digested by Hind III, which has no restriction site within the Helena sequence, so that each hybridized fragment would correspond to a single genomic insertion. The restricted fragments were separated in 1% agarose gels, and transferred to Hybond N+ membranes (Amersham Biosciences, Little Chalfont, UK). Blots were prehybridized for 1 h at 60°C in 5× SSC, in 5% dextran sulfate, subjected to 20-fold dilution of the liquid block, and hybridized overnight with the probes. Blots were washed twice with 0.2× SSC, 0.5% SDS, and then exposed to autoradiographic film for 20 minutes at room temperature. A 644 bp Helena sequence amplified from plasmid AF012044 (DsechF: 5' AGGATTTGTCATGCCACGCT 3' e DsechR R 5' TGTTTGGTGCTGCCATGTGT 3'), and a 674 bp sequence, corresponding to the RTase of D. mojavensis Helena (DmojF: 5' TAAGAGGCCATAGTACGGAGCAGGTA3' and DmojR: 5' GCGAATTGGAACAGGCTAACGCAT 3'), were used as probes for the D. simulans and D. mojavensis populations, respectively.
Quantitative RT-PCR (qRT-PCR)
The expression profiles of Helena RTase in different populations of D. simulans and D. mojavensis (Additional File 8) were determined by real-time PCR. For this analysis, 20 ovaries and 15 carcasses of each population were used to extract total RNA using RNeasy kit (Qiagen). 1 μg of total RNA treated with DNAse Ambion was converted into cDNA using Thermoscript kit (Invitrogen) primed with oligo-dt and random primers mix. The cDNA samples were diluted 50 fold, and PCR was carried out using QuantiTect SYBR Green PCR kit (Roche) on the LightCycler (Roche) using primers specific to the Helena RTase of D. simulans (RTase_D.simF: 5'ACAGCAGAGAGACAGCTAACGGAC 3', Rtase_DsimR: 5' AGATGTGTTGCTTGCAGGGTCTGA 3' and D. mojavensis (RTase_DmojF: 5' TTGGTCCGCTGCTGTTCTCCTT 3', Rtase_DmojR: 5' TGAGATTCCACCGCTTGCACCA 3') that amplify 193 bp and 204 bp respectively. Quantitative PCR cycling conditions were 5 min at 95°C (1 cycle), 15 s at 95°C, followed by 10 s at 62°C and 20 s at 72°C (50 cycles). A negative control for DNA contamination of cDNA of each population (without Thermoscript enzyme) was tested (data not shown). Reactions were done in duplicate, and standard curves were calculated from serial dilutions of specific amplified PCR fragments. The quantity of the transcripts was estimated relative to the RP49 expression (qPCR fragments of 182 bp and 167 bp for D. simulans and D. mojavensis respectively). Primers were: RP49_DsimF: 5' CGGATCGATATGCTAAGCTGT 3', RP49_DsimR: 5' GCGCTTGTTCGATCCGTA 3', RP49_DmojF: 5' GTCGTCGCTTCAAGGGCCAAT 3', RP49_DmojR: 5' ATGGGCGATCTCACCGCAGTA 3'. In both species, RP49 expression is equivalent (Additional Figure nine) allowing relative expression of Helena to be comparable between D. mojavensis and D. simulans. Hence, Helena transcripts plotted in Figure 6 are the result of quantification of Helena transcripts normalized by the quantification of RP49 transcripts for each strain.
DOA:
long interspersed nuclear element
LTR:
long terminal repeat
ORF:
TE:
transposable element
RTase:
reverse transcriptase.
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Funding for this project was provided by the Brazilian agencies FAPESP (07/53097-0 to C.M.A.C. and fellowship 07/50641-1 to A.G.) and CNPq (to C.M.A.C.), and by the French MIRA project from the Région Rhône-Alpes (to C. V. and A. G.), the ANR Genemobile (to C. V.), and the CNRS-FAPESP (joint program to C.V. and C.M.A.C.). We thank R. Rebollo, N. Burlet and C. Loevenbruck for their comments and technical help, and M. Ghosh for correcting the English text.
Laboratory of Molecular Evolution, Department of Biology, UNESP – São Paulo State University, 15054-000, São José do Rio Preto, São Paulo, Brazil
Adriana Granzotto, Fabrício R Lopes & Claudia MA Carareto
Université de Lyon, F-69000, Lyon
Emmanuelle Lerat & Cristina Vieira
CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, Université Lyon 1, F-69622, Villeurbanne, France
Adriana Granzotto
Fabrício R Lopes
Emmanuelle Lerat
Cristina Vieira
Claudia MA Carareto
Correspondence to Claudia MA Carareto.
AG carried out the molecular and genetic studies, AG, FRL and EL did the bioinformatic analyses, CV and CMAC designed and coordinated the study. All the authors contributed to data analyses and the writing of the paper. All the authors have read and approved the final manuscript.
Adriana Granzotto, Fabrício R Lopes, Emmanuelle Lerat, Cristina Vieira and Claudia MA Carareto contributed equally to this work.
Sequences of the
Additional file 1: Helena reference copies. Helena sequences used to determine the structure of the element, for each species. (PDF 26 KB)
copies in the
Additional file 2: Helena copies in the Drosophila sechellia sequenced genome. The data provided is a list of D. sechellia copies. (DOC 246 KB)
Additional file 3: Helena copies in the Drosophila erecta sequenced genome. The data provided is a list of D. erecta copies. (DOC 32 KB)
Additional file 4: Helena copies in the Drosophila ananassae sequenced genome. The data provided is a list of D. ananassae copies. (DOC 70 KB)
Additional file 5: Helena copies in the Drosophila yakuba sequenced genome. The data provided is a list of D. yakuba copies. (DOC 52 KB)
Additional file 6: Helena copies in the Drosophila virilis sequenced genome. The data provided is a list of D. virilis copies. (DOC 38 KB)
Additional file 7: Helena copies in the Drosophila mojavensis sequenced genome. The data provided is a list of D. mojavensis. (DOC 74 KB)
Schematic representation of
Additional file 8: Helena. Schematic representation of Helena copies in D. sechellia, D. yakuba, D. erecta, D. ananassae, D. mojavensis and D. virilis. The sequences represented have at least 90% identity and 50% of the length of the reference copy, and with e-values of less than 10e-10. Spaces = indels. The first schematic representation is the reference copy in each species. White = gag. Gray = RTase. (TIFF 4 MB)
Species and strains, geographic origin and year of collection
Additional file 9: . The data provided is a list of D. simulans and D. mojavensis used in this study for analysis of Helena activity in natural populations. (DOC 42 KB)
Mean CT of the
Additional file 10: rp49 control gene and Helena. Ct comparison between rp49 (reference) and Helena real time PCR assays of D. simulans and D. mojavensis. Gray = mean CT of D. simulans. Black = mean CT of D. mojavensis. Std = standard deviation. (TIFF 381 KB)
Granzotto, A., Lopes, F.R., Lerat, E. et al. The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes. BMC Evol Biol 9, 174 (2009). https://doi.org/10.1186/1471-2148-9-174
Drosophila Genome
Internal Deletion
Insertion Polymorphism
Active Copy
Genomic Environment

redpajama_set_name: RedPajamaCommonCrawl


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Hailed as one of the most exciting new singer-songwriters on the acoustic music scene, Matthew Shepherd is a 24 year-old singer-songwriter who has been delighting audiences in London, Essex and beyond with his collection of self penned songs. His debut studio album 'Top of the tree' was released in June 2015 and only took a day for the album to break into the iTunes Top 100 Singer/Songwriter Album Chart. His songs cover the happiness and heartbreak of love, with his warm, intimate and delicate style.
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Folk on the Dock (Liverpool), Loughton Folk Club, Crawley Folk Festival, The Harrison (London), The Troubadour (London), Ronnie Scott's (London), Pizza Express (London), Epping Folk Club, The Folkroom, Cliffs Pavilion (Southend-on-Sea), Woodfest, Aldeburgh Festival, Rickmansworth Festival, The Bedford (London), The Hawth Theatre, Sail Loft Folk Club, Stereo 92, Cult Café Ipswich, Kokomo Guildford, The Garage Islington, Westfield's Stratford City, Proud Camden, Black Book Café Stroud. Isle of Wight: The Anchor Inn, The Lifeboat, The Piano Café.
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How cool is this wedding!? I haven't seen anyone else get married on the USS Alabama before, this is definately a dream wedding for a photographer. For those people who got married on this same weekend in Pensacola, all of the weddings were rained out, but we had a lot of "good luck" charms around, from people praying for 2 straight days for no rain, a man who brought his umbrella because everytime he brings it he would never use the darn thing… and myself because (I am so going to jinx myself) I've only had one wedding that was rained out since I started documenting weddings!
Many many congratulations to Christo and Amber and a sincere thank you for letting me come along and capture this day for them.
This is so rad!! What a unique place to have a wedding!
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What a super fun location for a wedding!
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What a great idea for a wedding! The details, colors, and their personalities just explode against the haze grey.
Fantastic! You did a wonderful job of capturing the spirit of the day and the people attending! So cool!
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I can't even explain how amazing these are. I served in the Navy for 4 years and you make ships look way more beautiful than I remember them being! I love how creative this couple was in having their wedding there.
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This Class is also called Rhombohedric-hemimorphic Class, because the number of faces of the Forms of this Class is half that of the Rhombohedric Hemihedric Division.
One 3-fold rotation axis, coincident with the c axis, i.e. the vertical crystallographic axis. The axis is polar.
The Forms of this Class will be found when we subject the Forms of the Trigonal Hemihedric Division (described in Part Eight) to hemimorphy, which means that we suppress their equatorial mirror plane.
From the trigonal hemihedric trigonal bipyramid can be derived the trigonal monopyramid when we subject it to hemimorphy. The bipyramid decays in two independent halves -- monopyramids -- an upper and a lower one. See Figure 1. Both monopyramids are open Forms, which here means that such a pyramid is not closed by a basal (horizontal) face, it just consists of three faces. In figure 1a one such monopyramid is shown.
The symbol "2 h" in the Naumann expression means that hemimorphy is applied to the trigonal hemihedric trigonal bipyramid.
Figure 1. Derivation of two possible Trigonal Monopyramids from the trigonal hemihedric Trigonal Bipyramid, by suppression of the equatorial mirror plane, expressed by the suppression of either the lower faces (indicated as red) or the upper faces (indicated as white).
Figure 1a. The Trigonal Monopyramid derived from the white faces of the bipyramid of Figure 1.
From the trigonal hemihedric type II hexagonal bipyramid can be derived two type II hexagonal monopyramids. These also are open Forms. See Figure 2 and 2a.
Figure 2. Derivation of the hemimorphous trigonal hemihedric Type II Hexagonal Monopyramid from the trigonal hemihedric Type II Hexagonal Bipyramid.
The colors symbolize the symmetries involved. Two independent Monopyramids are possible depending on the suppression of either the yellow/white faces (= upper faces) or of the green/blue faces (= lower faces). The latter possibility is depicted in Figure 2a.
Figure 2a. The hemimorphous trigonal hemihedric Type II Hexagonal Monopyramid derived from the yellow/white faces of the right bipyramid of Figure 2.
From the ditrigonal bipyramid can be derived the ditrigonal monopyramid by applying hemimorphy to it. See Figure 3.
Figure 3. Derivation of hemimorphous Ditrigonal Monopyramids from a Ditrigonal Bipyramid.
Figure 3a. The hemimorphous Ditrigonal Monopyramid derived from the white faces of the right bipyramid of Figure 3.
From the trigonal prism can be derived a hemimorphous Trigonal Prism, which has the same external shape but lacks equatorial symmetry. See Figure 4.
Figure 4. The hemimorphous Trigonal Prism.
From the trigonal hemihedric type II hexagonal prism can be derived a hemimorphous (trigonal hemihedric) type II hexagonal prism. It has the same shape. The suppression of the equatorial mirror plane does not lead to a loss of faces. See Figure 5.
Figure 5. The hemimorphous (trigonal hemihedric) Type II Hexagonal Prism derived from the trigonal hemihedric type II Hexagonal Prism.
From the ditrigonal prism we can derive the hemimorphous ditrigonal prism. Its shape remains the same, because the suppresion of the equatorial mirror plane does not cause a loss of faces. See Figure 6.
Figure 6. Derivation of the hemimorphous (trigonal hemihedric) Ditrigonal Prism from the trigonal hemihedric Ditrigonal Prism.
From the trigonal hemihedric basic pinacoid we can derive the pedion. The latter is a single horizontal face : by suppressing the equatorial mirror plane of the basic pinacoid we get in fact two such faces, i.e. two independent horizontal faces, an upper one and a lower one. Such a pedion can close a monopyramid by forming its base. Two such pedions (an upper and a lower one) could close a prism. See Figure 7, 7a and 7b.
Figure 7. The trigonal hemihedric Basic Pinacoid (derived from the holohedric Basic Pinacoid) will be subjected to hemimorphy, resulting in two Pedions (Monohedrons) which are depicted in the next two Figures.
The system of four crystallographic axes is indicated (red lines).
Figure 7a. Upper Pedion, derived from the trigonal hemihedric Basic Pinacoid, by suppression of the equatorial mirror plane, expressed by the removal of the lower face.
Figure 7b. Lower Pedion, derived from the trigonal hemihedric Basic Pinacoid, by suppression of the equatorial mirror plane, expressed by the removal of the upper face.
This concludes our derivation of all the Forms of the Ditrigonal-pyramidal Crystal Class by means of the merohedric approach.
We will now derive those same Forms by subjecting the basic faces (compatible with the Hexagonal Crystal System) one by one to the symmetry operations of the present Class (the Ditrigonal-pyramidal Crystal Class).
Figure 8. Stereogram of the symmetry elements of the Ditrigonal-pyramidal Crystal Class, and of all the faces of the most general Form.
The face a : ~a : -a : c generates a trigonal monopyramid when subjected to the symmetry elements of the present Class : The action of the 3-fold rotation axis multiplies the face by three. Then the mirror plane symmetry is already implied (i.e. it does not add any new faces anymore). See Figure 9.
(1). Position of the face a : ~a : -a : c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
(2). Stereogram of the generated Trigonal Monopyramid.
The face 2a : 2a : -a : c generates a hemimorphous (trigonal hemihedric) Type II hexagonal monopyramid when subjected to the symmetry elements of the present Class : The action of the 3-fold rotation axis multiplies this face by three. Each of these faces is then duplicated by a nearby mirror plane. See Figure 10.
(1). Position of the face 2a : 2a : -a : c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
(2). Multiplication by three in virtue of the action of the 3-fold rotation axis.
(3). Duplication of each face in virtue of the mirror planes.
The face [3/2]a : 3a : -a : c is a definite face. Here we consider its general version, namely the face [s/(s-1)]a : sa : -a : c. It has a general position within the stereographic projection, and generates a ditrigonal monopyramid when subjected to the symmetry elements of the present Class : The nearby mirror plane duplicates this face, and the resulting face pair is multiplied by three in virtue of the 3-fold rotation axis.
(1). Position of the face [s/(s-1)]a : sa : -a : c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
(2). Duplication of the face in virtue of the nearby mirror plane, resulting in a face pair.
(3). Multiplication of this face pair by three in virtue of the action of the 3-fold rotation axis, resulting in a Ditrigonal Monopyramid.
The face [3/2]a : 3a : -a : ~c is a definite one. Here we consider its generalized version, namely the face [s/(s-1)]a : sa : -a : ~c. Its orientation is vertical. It will generate a ditrigonal prism when subjected to the symmetry elements of the present Class : The nearby mirror plane will duplicate this face, resulting in a face pair. Then the 3-fold rotation axis will multiply this face pair by three resulting in six vertical faces, the ditrigonal prism. See Figure 12.
(1). Position of the face [s/(s-1)]a : sa : -a : ~c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
(2). Duplication of the face in virtue of the nearby mirror plane, resulting in a vertical face pair.
(3). Multiplication of this face pair by three in virtue of the action of the 3-fold rotation axis, resulting in a Ditrigonal Prism.
The face a : ~a : -a : ~c is also vertical. It generates a trigonal prism when subjected to the symmetry elements of the present Class : The 3-fold rotation axis triples the face, and then the other symmetries of the Class (the mirror planes) are already implied. The result is three vertical faces, the trigonal prism. See Figure 13.
(1). Position of the face a : ~a : -a : ~c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
(2). Multiplication of this face by three in virtue of the action of the 3-fold rotation axis, resulting in a Trigonal Prism.
The face 2a : 2a : -a : ~c is also vertical. It generates a hemimorphic trigonal hemihedric type II hexagonal prism when subjected to the symmetry elements of the present Class : The mirror plane to the left of it duplicates this face, and the resulting vertical face pair is multiplied three times in virtue of the 3-fold rotation axis. So now we have 6 vertical faces making up the hexagonal prism. See Figure 14.
(1). Position of the face 2a : 2a : -a : ~c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
(2). Duplication of this face by the action of a mirror plane.
(3). Multiplication of the resulting face pair by three in virtue of the action of the 3-fold rotation axis, resulting in a Hexagonal Prism.
The face ~a : ~a : ~a : c is horizontal. Neither the 3-fold rotation axis, nor the mirror planes have any effect on it (i.e. they do not generate new faces), so this Form consists just of the one face we started with and is caled a pedion. See Figure 15.
Position of the face ~a : ~a : ~a : c in the stereographic projection of the symmetry elements of the Ditrigonal-pyramidal Crystal Class.
When we subject it to the symmetry elements of the present Class no new faces are generated, so we end up with a Pedion.
This concludes our exposition of the Ditrigonal-pyramidal Crystal Class.
To continue, click HERE for Part Ten (Class 6* ).


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