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Streetwise Reports' Article Archives — October 2017 back to current month (65)
'Shovel-Ready' Project Could Benefit from Rebounding Rare Earth Prices (10/31/2017)
As China's central government clamps down on archaic, polluting mines, Australia looks to meet surging global demand.
Alaska Gold Mega-Project on Track (10/31/2017)
The final environmental report from the U.S. Army Corps of Engineers is expected to be delivered in 2018.
Gold Major Positioned as 'Preferred Large Gold Producer Under Coverage' (10/31/2017)
BMO Capital Markets reported this producer's progress during Q3/17.
Excelsior Mining Very Close to Being Fully Permitted, Re-rating Awaits (10/30/2017)
Excelsior Mining continues to hit permitting milestones, says The Critical Investor, who profiles the company.
Gold Could Go Lower on Dollar 'Swan Song' Rally (10/30/2017)
Technical analyst Clive Maund charts gold's move in relation to the U.S. dollar.
Is Silver's Underperformance a Positive Sign? (10/30/2017)
Technical analyst Clive Maund explains what he believes silver's prolonged underperformance signals.
Rebirth in Nevada for Gold Explorer (10/29/2017)
Michael Ballanger examines a company with an earn-in agreement for a project at the confluence of three gold trends in Nevada.
Jack Chan's Weekly Precious Metals Market Update (10/28/2017)
Technical analyst Jack Chan charts the latest moves in the gold and silver markets.
Dollar Breaks Out (10/27/2017)
Technical analyst Clive Maund discusses the outlook for the dollar, euro and precious metals sector after the predicted major dollar breakout occurs.
Metrics 'Encouraging' for Q1/18 Commercial Gold Production (10/26/2017)
A Macquarie Research report finds many leading indicator metrics promising for early 2018 commercial production at a Nevada gold mine.
Major with Cohort of Mexican Mines Is On Track to Achieve 2017 Production Guidance (10/26/2017)
BMO Capital Markets reviewed this silver and gold miner's Q3/17 production results.
Q3 Production Results Have Analysts Watching and Waiting for What This Gold Miner Does Next (10/26/2017)
With guidance projections being met, analysts are looking forward to Q4 results from this gold miner.
Biopharmaceutical Firm Receives FDA Priority Review and PDUFA Date (10/25/2017)
Neil Maruoka, an analyst with Canaccord Genuity, provided an update on this biotech's progress through the regulatory approval process for one of its therapeutics.
Analyst Calls Biotech with Alzheimer's Therapy a 'High Risk, High Reward Opportunity' (10/25/2017)
Mackie Research reported on this company's recent announcement about one of its product candidates targeting Alzheimer's disease.
Coverage Initiated on Gene Therapy Company (10/25/2017)
Analyst David Nierengarten with Wedbush explained the rationale for adding this biotech to his coverage universe.
Biotech Receives Device Approval in Five More Countries; Revenue Up 122% YOY (10/25/2017)
Maxim Group reported on this firm's Q3/17 earnings, as well as its progress in obtaining international regulatory clearances for its medical system.
Oil & Gas Firm Offers 'High-Impact Exploration Potential' (10/24/2017)
Mackie Research provided the recent results from one of the exploration wells this energy company co-owns in Indonesia.
This Company Is Jumping on the Electric Vehicle Revolution (10/24/2017)
Anoop Prihar, an analyst with GMP Securities, explained the investment thesis for this metals corporation that began trading during the summer.
Will the Dollar Stop Oil's Rise? (10/24/2017)
Technical analyst Clive Maund discusses the signals for oil that he sees in the charts.
Q3/17 Mexican Silver Production Report Shines (10/24/2017)
A producer/explorer active in Mexico and Peru released third quarter production results that were in line with one analyst's expectations.
Jack Chan's Weekly Precious Metals Update (10/23/2017)
Dollar Getting Ready to Rally (10/22/2017)
Technical analyst Clive Maund discusses the outlook for the U.S. dollar and its implications for gold.
Silver Is Underperforming Gold (10/22/2017)
Technical analyst Clive Maund charts silver and discusses its relationship with gold.
Reverse Takeover for Explorer in the Golden Triangle (10/19/2017)
A company chaired by mining veteran Larry Nagy that has rights to a project in northern British Columbia's Golden Triangle has completed a reverse takeover and begins trading on the TSX Venture Exchange on Oct. 19.
Miner Exceeds Estimates for Gold Produced in Q3/17 (10/19/2017)
BMO Capital Markets reported the preliminary Q3/17 production results for this senior gold producer.
Biotech Announces 'Late-Breaking' Addition to Immunotherapy Conference (10/18/2017)
Michael King Jr., an analyst with JMP Securities, reported this biopharmaceutical firm was recently added to the roster of presenters scheduled for an upcoming annual meeting.
Biotech Releases Initial Zika Vaccine Trial Results; Advances Combo Trials (10/18/2017)
With multiple candidates in the pipeline advancing through clinical trials, Ram Selvaraju, an analyst with H.C. Wainwright & Co., provided insight into developments at a biotech addressing Zika virus, advanced bladder cancer and cervical dysplasia.
Columbus Gold Is Giving Free Shares Away (10/18/2017)
Bob Moriarty of 321 Gold discusses Columbus Gold's imminent spinout of its Nevada properties.
NASDAQ-Listed Blockchain Company Acquires TESS (10/17/2017)
One company continues to invest in the blockchain industry by acquiring an ownership interest in a firm developing a payment system for the telecom industry.
Gold at Year-End and a Lesson in Shorting (10/17/2017)
Ron Struthers of Struthers' Resource Stock Report provides his outlook on gold stock performance going into the end of the year, offers a lesson in Shorting 101, and discusses his views on particular companies.
Cannabis Cultivator Poised to Profit When Recreational Marijuana Becomes Legal in Canada (10/17/2017)
With the Canadian cannabis market primed to be worth billions upon legalization of recreational marijuana use, Lior Gantz of Wealth Research Group makes the case for an "undervalued" competitor.
Trio of Firm's Mines Log Strong Performance in Q3/17 (10/16/2017)
Analyst Andrew Kaip compared this silver, gold and base metals miner's reported Q3/17 production results to the numbers expected by his firm, BMO Capital Markets.
Comfortably Numb: Surviving the Assault on Silver (10/15/2017)
Precious metals expert Michael Ballanger discusses the current state of the precious metals markets.
Gold and the Dollar: Good News and Bad News (10/15/2017)
Technical analyst Clive Maund discusses what he sees as a "swan-song rally."
Silver May Pop and Drop in Setup for Upswing (10/15/2017)
Technical analyst Clive Maund charts the reasons silver bugs may have an opportunity for happier days ahead.
Future Upside Provides Excellent Entry Point for Fiore Gold (10/13/2017)
The Critical Investor provides an update on Fiore Gold, which has just resumed trading after completing a business combination with GRP Minerals.
An Aggressive Gold Exploration Play (10/12/2017)
Exploration on a massive project in Nevada holds the potential for "glory," says Daniel Ameduri of Future Money Trends.
Reliq Health Technologies Quickly Gaining Traction in Fast Growing Telemedicine Market (10/11/2017)
The rapid increase in the incidence of chronic diseases and the need to reduce healthcare costs have led to the growth in telemedicine, and Reliq Health Technologies is adding thousands of patients to its rolls.
Noted Geologist Peter Megaw Believes This Property Hosts the Source of Arizona Mining's Taylor Deposit (10/11/2017)
A project in Arizona about to undergo systematic exploration offers the potential of polymetallic resources including zinc.
NASDAQ-Listed Blockchain Company on the Move (10/11/2017)
A company that has shifted its focus to blockchain technology has garnered the interest of investors.
The Electric Car Blindsides the Automakers (10/11/2017)
With demand for electric cars on the rise, Ron Struthers of Struther's Resource Stock Report examines the lithium market and one company that he believes is in the right place.
Uranium Explorer Adds Mineralization to 'World-Class Project' (10/10/2017)
A company that has tallied up a series of impressive uranium discoveries on its Athabasca Basin property reported positive results for another 22 drill holes, and a pair of analysts have taken note.
Oil Breaking Out, But the Money Is Elsewhere (10/09/2017)
Tom Beck, founder of Portfolio Wealth Global, discusses the energy sector and highlights one commodity that he says appreciates when oil becomes overpriced.
Are Gold and the Dollar Rallying Together? (10/08/2017)
Technical analyst Clive Maund examines the relationship between the dollar and precious metals.
Is Silver Turning? (10/08/2017)
Technical analyst Clive Maund discusses movements in the silver price.
What Happened to the Crow? (10/08/2017)
Bob Moriarty of 321 Gold expounds on a theory on where the gold in Australia's Pilbara region came from.
If 'Bottoming Pattern' Develops, Oil Bear Market May Be Over (10/07/2017)
Technical analyst Jack Chan charts the latest moves in the oil and gas markets.
Cannabis Company Forms Partnership to Develop Pharmaceuticals (10/06/2017)
Analyst Russell Stanley with Echelon Wealth Partners explained the significance of the recent licensing agreement signed by this Canadian medical marijuana producer and distributor.
Telemedicine Platform Is Quickly Gaining Traction in Fast Growing Market (10/05/2017)
The rapid increase in the incidence of chronic diseases and the need to reduce healthcare costs have led to the growth in telemedicine, which has companies racing to bring new products to market.
Reliq Health Technologies Quickly Gaining Traction in Rapidly Growing Telemedicine Market (10/05/2017)
Flying Drones into a $32 Billion Market Opportunity (10/05/2017)
A Canadian company is expanding quickly in providing drone services to the agriculture industry in Canada and the U.S.
Graphite Seeing Some Upward Movement (10/05/2017)
With graphite prices starting to move upward, Ron Struthers of Struther's Resource Stock Report provides a primer on graphite and discusses his evaluation method for graphite companies.
Genesis Metals: Chevrier Phase 1 Drilling Completed, Phase 2 Program Commencing (10/05/2017)
The Critical Investor provides an update on Genesis Metals' drill results and the company's next steps.
Left Coast Zinc Hunt Leads to California (10/05/2017)
Zinc is on a hot streak right now, says Chris Parry of Equity Guru, who profiles one company that just announced a resource update for its California project.
Japan Is Fertile Ground for Biotech Deals (10/04/2017)
Japan and the United States are hotspots for companies that are looking to license-out exciting regenerative medicine technology, says Colin Lee Novick, managing director of CJ PARTNERS. In this interview with The Life Sciences Report, Novick discusses a regenerative medicine company pursuing partnerships in Japan for its cell therapies to treat chronic tendinosis, damaged or aged skin, and pattern baldness.
Small Biotech Enters Patent Deal with British Company Targeting Schizophrenia (10/04/2017)
A small-cap American biotech has secured a patent agreement with a larger British firm, garnering the attention of three sector analysts.
Demand for Cobalt Focuses Analyst's Attention on Streaming Company (10/03/2017)
A "large appetite" for cobalt, in demand for use in lithium ion batteries powering electric vehicles, contributed to a decision to initiate coverage on this firm.
Fire Prevention Company 'Undervalued, Underfollowed Play on New Safety Requirements' (10/03/2017)
Russell Stanley, an analyst with Echelon Wealth Partners, explained the significance of the milestone for this cooking fire prevention technology firm.
A Zinc Resource Update That Is Over the Moon (10/03/2017)
This company with a zinc project in California just came out with an updated resource, one which Etienne Moshevich of Alphastox.com calls "outstanding."
Visit to Vast Nunavut Exploration Camp Highlights Possibilities (10/03/2017)
James Kwantes of Resource Opportunities takes an in-depth look at a "promising" Canadian explorer.
The Monotonous Madness of Managed Markets (10/03/2017)
Precious metals expert Michael Ballanger discusses capital destruction in precious metals and banking.
Novo Starts Major Test Program (10/01/2017)
Bob Moriarty of 321 Gold discusses the core drill program that Novo Resources has undertaken.
A Tiny Junior Targeting 1-3 Moz High-Grade Gold Left Behind in the Past-Producing Idaho-Maryland Mine (10/01/2017)
The Critical Investor profiles a company tackling a gold project in California that halted production when it was nowhere near depletion.
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– Rick Mills, Ahead of the Herd
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Is this a joke? I would have needed to contact them for EVERY issue I received in the last 5 years.
LOL, I know right? I can count on one hand the number of issues I've ever gotten within 14 days, and I probably wouldn't need all the fingers, and that's going back maybe a decade or more.
Issue #190 arrived to me yesterday here in the states, Las Vegas to be exact. So it took about 25 days or so since #189 had arrived, so not as good as it used to be, but still in the OK range.


text:
Q: Lifetime error for method returning iterator I am facing a weird lifetime compilation error while trying to compile following simple XOR encryption Rust code.
pub struct Encrypt<'a> {
    key:&'a[u8],
    current_index: usize,
}

impl<'a> Encrypt<'a> {
    pub fn new<T: ?Sized + AsRef<[u8]>>(key: &'a T) -> Encrypt<'a> {
        Encrypt { key: key.as_ref(), current_index: 0 }
    }

    pub fn encrypt<T, U>(&'a mut self, data: T) -> impl Iterator<Item = u8>
                where T: IntoIterator<Item = U>, U: std::borrow::Borrow<u8> {
        let iter = data.into_iter().map(|b| {
            let val = b.borrow() ^ self.key[self.current_index];
            self.current_index = (self.current_index + 1) % self.key.len();
            val
        });
        iter
    }
}

I am getting following bompilation error:
error[E0700]: hidden type for `impl Trait` captures lifetime that does not appear in bounds
8  | impl<'a> Encrypt<'a> {
   |      -- hidden type `Map<<T as IntoIterator>::IntoIter, [closure@src/lib.rs:36:41: 40:10]>` captures the lifetime `'a` as defined here
...
41 |         iter

This is really strange because the method encrypt() does not return any reference, still the compiler appears to be associating the lifetime of self with it.

A: This is a common problem that can arise from incorrect lifetime annotations. The issue is that you're binding the returned iterator's lifetime to the lifetime of self which is 'a. But you don't care about the lifetime of self, you only care about the lifetime of the reference you're borrowing to it, &mut self, let's call that lifetime 's.
Now we just need to tell the compiler:
impl<'a> Encrypt<'a> {
    pub fn encrypt<'s, T, U>(&'s mut self, data: T) -> impl Iterator<Item = u8> + 's
    //             ^^         ^^                                                ^^^^
    // tie the return value's lifetime to the lifetime for which we burrow `self`
    // instead of the whole lifetime of `self`.
    where
        T: IntoIterator<Item = U> + 's,
        //                        ^^^^
        // That'll transitively require this bound
        U: std::borrow::Borrow<u8>,
    {
        let iter = data.into_iter().map(|b| {
            let val = b.borrow() ^ self.key[self.current_index];
            self.current_index = (self.current_index + 1) % self.key.len();
            val
        });
        iter
    }
}

redpajama_set_name: RedPajamaStackExchange


text:
package parameters

// Option value modes.
const (
	OptionValueNone OptionValueMode = iota
	OptionValueOptional
	OptionValueRequired
)

// OptionValueMode represents the different potential requirements of an option's value.
type OptionValueMode int

// Option provides the internal representation of an input option paremeter.
type Option struct {
	// The names of this option.
	Names []string
	// The description of this option.
	Description string
	// The name of an environment variable to read an option value from.
	EnvVar string
	// The value that this option references.
	Value Value
	// Does this option take a value? Is it optional, or required?
	ValueMode OptionValueMode
	// The name of the value (shown in contextual help).
	ValueName string
}

redpajama_set_name: RedPajamaGithub


text:
Escherichia coli Induces DNA Double-Strand Breaks in Eukaryotic Cells
Jean-Philippe Nougayrède1,
Stefan Homburg2,
Frédéric Taieb1,
Michèle Boury1,
Elzbieta Brzuszkiewicz3,
Gerhard Gottschalk3,
Carmen Buchrieser4,
Jörg Hacker2,
Ulrich Dobrindt2,
Eric Oswald1,*
1 INRA, UMR1225, Ecole Nationale Vétérinaire de Toulouse, Toulouse F-31076, France.
2 Institut für Molekulare Infektionsbiologie, Universität Würzburg, Würzburg D-97070, Germany.
3 Göttingen Genomics Laboratory, Universität Göttingen, Göttingen D-37077, Germany.
4 Institut Pasteur, Unité de Génomique des Microorganismes Pathogènes and CNRS URA 2171, Paris F-75724, France.
↵* To whom correspondence should be addressed. E-mail: e.oswald{at}envt.fr
Science 11 Aug 2006:
Vol. 313, Issue 5788, pp. 848-851
DOI: 10.1126/science.1127059
Jean-Philippe Nougayrède
INRA, UMR1225, Ecole Nationale Vétérinaire de Toulouse, Toulouse F-31076, France.
Stefan Homburg
Institut für Molekulare Infektionsbiologie, Universität Würzburg, Würzburg D-97070, Germany.
Frédéric Taieb
Michèle Boury
Elzbieta Brzuszkiewicz
Göttingen Genomics Laboratory, Universität Göttingen, Göttingen D-37077, Germany.
Gerhard Gottschalk
Carmen Buchrieser
Institut Pasteur, Unité de Génomique des Microorganismes Pathogènes and CNRS URA 2171, Paris F-75724, France.
Jörg Hacker
Ulrich Dobrindt
Eric Oswald
For correspondence: e.oswald@envt.fr
Transient infection of eukaryotic cells with commensal and extraintestinal pathogenic Escherichia coli of phylogenetic group B2 blocks mitosis and induces megalocytosis. This trait is linked to a widely spread genomic island that encodes giant modular nonribosomal peptide and polyketide synthases. Contact with E. coli expressing this gene cluster causes DNA double-strand breaks and activation of the DNA damage checkpoint pathway, leading to cell cycle arrest and eventually to cell death. Discovery of hybrid peptide-polyketide genotoxins in E. coli will change our view on pathogenesis and commensalism and open new biotechnological applications.
Escherichia coli is both the most common cause of infections by Gram-negative bacilli and a commensal of the normal gut microflora (1). The versatility of this pathogen arises from production of a diverse array of virulence factors that manipulate basic host cell functions (2, 3), such as the cyclomodulins, which target the host cell cycle and influence whether an infected cell will grow and divide, or die (4). We observed that certain E. coli strains induce megalocytosis in cultured eukaryotic cells, characterized by a progressive enlargement of the cell body and nucleus and the absence of mitosis (Fig. 1). This cytopathic effect, reminiscent of the effect of cyclomodulins, was observed upon transient infection of different mammalian cells (HeLa, CHO, A375, and IEC-6) and induced by pathogenic E. coli strains isolated from meningitis and urinary tract infections and by certain commensal strains but not by laboratory K-12 strains nor by enteropathogenic or enterohemorrhagic E. coli (Fig. 1A, fig. S1, and table S1). The cytopathic activity was contact-dependent and was not observed when bacteria were separated from mammalian cells by a 0.2-μm permeable membrane. Inhibition of bacterial internalization with cytochalasin-D did not abrogate the cytopathic effect. Heat-killed bacteria, gentamicin-killed bacteria, bacterial culture supernatants, and bacterial lysates were not cytopathic (fig. S2). This effect could not be explained by the production of toxins known to alter the host cell cycle such as Cytolethal Distending Toxins (CDT) (5), Cycle Inhibiting Factor (6), or Cytotoxic Necrotizing Factors (7), or by the production of α-hemolysin (8). Engineered mutants and strains devoid of these toxin genes remained cytopathic (table S1).
Morphologic changes were induced upon transient infection of epithelial cells with E. coli. (A) Live pathogenic E. coli strain IHE3034 or laboratory strain DH10B was added directly onto HeLa cells, cocultivated for 4 hours, then washed. The cells were incubated for 72 hours with gentamicin before staining with Giemsa. Scale bars, 100 μm. (B) Cell nuclei (blue), F-actin (red), and α-tubulin (green) demonstration 6 to 72 hours after transient infection with IHE3034 (bottom) or without IHE3034 (top). Scale bars, 40 μm.
To identify the bacterial genes involved in this phenotype, we generated transposon mutants in two cytopathic E. coli strains. Negative mutants had transposons clustered in a 54-kilobase chromosomal region (Fig. 2) that exhibited typical features of a genomic island and was inserted in the asnW tRNA locus, an integration hotspot for foreign mobile DNA elements (2). The genomic island was fully sequenced in newborn meningitis strain IHE3034, and the presence of an identical genomic island was confirmed in newborn meningitis strain SP15 (9), commensal strain Nissle 1917 (10), and uropathogenic strain CFT073 (11). To show the involvement of this genomic island in the induction of the megalocytosis phenotype, we deleted the entire island in IHE3034, resulting in a noncytopathic mutant (fig. S3). In contrast, laboratory E. coli strain DH10B hosting a bacterial artificial chromosome (BAC) bearing the complete genomic island triggered megalocytosis and proliferation arrest in transiently infected cells, whereas DH10B harboring the empty BAC vector did not (fig. S3).
Schematic map of the 54-kb pks island. Localization of transposon insertions in strains IHE3034 and SP15 resulting in loss of the cyopathic effect are indicated by black and gray flags. Open reading frames (ORFs) whose gene products are involved in peptide-polyketide synthesis and cytopathic effect are indicated in different shades of blue (NRPS and PKS, dark blue; others, light blue). ORFs not strictly required for the cytopathic effect are shown in white. Transposase and integrase ORFs are shown in gray. ORF designations are given below the ORF symbols. Clb, colibactin. The predicted functions are shown above the ORF; ppt, phosphopantetheinyl transferase; nrps, nonribosomal peptide synthetase; pks, polyketide synthase; hcdh, hydroxyl acyl coA dehydrogenase; acp, acyl carrier protein; dhg, αβ dehydrogenase; at, acyl-transferase; am, amidase; te, thioesterase. The predicted domain organization of NRPS and PKS is indicated: A, adenylation; ACP/PCP, phosphopantetheine/acyl carrier; AT, acyltransferase; C, condensation; Cy, cyclization; ER, enoyl reductase; KR, ketoacyl reductase; KS, ketoacyl synthase; OX, oxidation.
To test the distribution of this genomic island within the species E. coli, we performed a survey on 55 intestinal pathogenic E. coli strains (enteroinvasive, enteropathogenic, enterohemorrhagic, enterotoxigenic, and enteroaggregative E. coli), 97 extraintestinal pathogenic E. coli (ExPEC) strains, and 32 strains isolated from the feces of healthy individuals. Polymerase chain reaction (PCR) screening indicated that this genomic island is absent in intestinal pathogenic E. coli strains, but present in 53 and 34% of the ExPEC and fecal isolates, respectively. Furthermore, PCR screening of the complete E. coli reference collection indicated that this genomic island is restricted to, and widely distributed in, the B2 phylogenic group that comprises commensals and ExPEC strains (12, 13) (fig. S4).
The genomic island, hereafter named pks island, encodes a machinery for the synthesis of peptide-polyketide hybrid compounds. This machinery consists of three nonribosomal peptide megasynthases (NRPS); three polyketide megasynthases (PKS); two hybrid NRPS/PKS megasynthases; and nine accessory, tailoring, and editing enzymes (table S2). NRPS and PKS are large multifunctional enzymes, found in bacteria and fungi, that produce an immense variety of peptides and polyketides of broad structural and biological activity (14, 15). In silico analysis of the megasynthases encoded by the genomic island revealed a typical but complex modular structure (Fig. 2). Noteworthy is the thiazole-forming NRPS module in ClbK (composed of heterocyclization, cysteine-specific adenylation, oxidation, and peptidyl carriage domains). Thiazole rings are signature pharmacophores common to many natural products and are important functional elements [e.g., intercalating DNA as in the case of the peptide-polyketide bleomycin (16)].
Systematic mutagenesis of the pks-island genes in DH10B harboring the pks island on a BAC (BACpks) showed that all of the PKS and NRPS and eight of the nine accessory and tailoring enzymes were required to induce the cytopathic effect (Fig. 2 and table S2). Only mutation of the gene coding for a putative efflux pump (17) did not alter the cytopathic activity, possibly because other efflux pumps encoded elsewhere on the chromosome could rescue this mutation. Reverse transcription PCR experiments indicated that the genes of the pks island were transcribed under in vitro conditions, as well as during contact with host cells (fig. S5). Together, these genetic and functional analyses indicate that the E. coli pks island codes for a polyketide-peptide hybrid cytotoxin.
To characterize the mode of action of this new cytotoxin, we examined the cell cycle of infected mammalian cells exposed to cytopathic E. coli strains. Flow cytometry analyses showed that the nucleus of the giant cells had a 4n DNA content (Fig. 3A). This observation, together with the absence of dividing cells (Fig. 1B), indicates that giant cells were blocked at the G2/M transition. Time-course experiments in which cells were synchronized at the G1/S transition and then exposed to bacteria showed that DH10B BACpks-exposed cells lagged in S phase for 48 hours and eventually accumulated in G2/M, whereas control cells went through S phase in less than 12 hours and continued a normal cell cycle (Fig. 3A). We examined whether the G2 checkpoint that stops the cell cycle in response to DNA injury was activated (18). Ataxia-telangiectasia mutated protein (ATM), a central protein in DNA damage response (19), was activated in DH10B BACpks-exposed cells together with the ATM signal-transducer Chk2 (Fig. 3B). Chk2 is known to phosphorylate Cdc25C protein, resulting in its inactivation by cytoplasmic retention by 14-3-3 proteins. As expected, we observed that Cdc25C was excluded from the nuclei of DH10B BACpks-exposed cells (Fig. 3C). Consistent with the nuclear exclusion of Cdc25C, we observed high levels of inactive phosphorylated (Tyr15) form of Cdk1 in DH10B BACpks-exposed cells (Fig. 3B), thus explaining the G2/M block. Further evidence that the G2 checkpoint is activated in cells exposed to cytopathic E. coli was obtained by inhibiting ATM with caffeine (20). The G2 block was alleviated, as a substantial number of cells reentered M-phase upon caffeine treatment (Fig. 3D). Hence, the DNA damage signaling cascade, starting with ATM activation, is activated upon exposure to E. coli harboring the pks island.
Transient infection with E. coli harboring the pks island induced cell cycle arrest and activation of the G2 checkpoint. (A) HeLa cells were synchronized in G1/S (Synchro) or left unsynchronized (Unsynchro), then infected 4 hours with DH10B harboring the BACpks or the vector alone. Cell cycle progression was monitored by flow cytometry at given times after infection. (B) G1/S-synchronized HeLa cells were infected as before and the activation of the DNA damage pathway was examined 48 hours after infection by Western blotting, with the use of antibodies that recognize the phosphorylated (p) forms of target proteins. As controls, cells were treated with etoposide and purified Cytolethal Distending Toxin (CDT), both known to activate the DNA damage cascade response. kDa, kilodaltons. (C) Cells were infected as in (B) and intracellular localization of Cdc25C was observed by confocal microscopy. Note Cdc25C cytoplasmic sequestration in giant cells, whereas in controls Cdc25C was found in nuclei of dividing cells (arrows). (D) G1/S-synchronized HeLa cells were infected, incubated for 42 hours, and further treated with or without caffeine for 6 hours. Cell cycle distribution was analyzed by bivariate flow cytometry for DNA content and mitotic phosphoproteins (MPM-2) to discriminate mitotic cells from G2 cells in the 4n population. Percentages of mitotic cells are shown.
To examine whether exposure to E. coli harboring the pks island inflicts DNA injury to host cells, we monitored the phosphorylation of histone H2AX, a sensitive marker of DNA double-strand breaks (DSBs) (21). Both HeLa cells and nontumor intestinal crypt IEC-6 cells exhibited nuclear phosphorylated H2AX (γH2AX) within 4 hours (Fig. 4A and fig. S6). The γH2AX signal of the infected DH10B BACpks cell population increased in a dose-related manner, ranging from distinctive nuclear foci to pan-nuclear response, reaching saturation at an infectious dose of 100 bacteria per cell (Fig. 4, A and B). Twenty-four hours after infection with low dose of DH10B BACpks (20 bacteria per cell), a subset of cells showed background levels of γH2AX (Fig. 4B), suggesting that these cells endured moderate DNA damage and repaired their DNA. The occurrence of DSBs in infected cells was confirmed with the use of the single-cell gel electrophoresis (comet) assay. Four hours after exposure to bacteria, DNA lesions were detected in cells exposed to DH10B BACpks (Fig. 4C). The comet tail moment increased with the number of infecting DH10B BACpks bacteria (Fig. 4D), indicating increased amounts of DSBs.
Exposure to E. coli harboring the pks island induced host DNA DSBs. (A) HeLa cells were infected with DH10B harboring the BACpks or empty vector [multiplicity of infection (moi) = 100] or with a 50:50 mix of each strain and examined 4 hours later for DNA (blue) and for phosphorylated H2AX (γH2AX) (green). Scale bars, 20 μm. (B) HeLa cells were infected 4 hours with given moi, then 4 to 24 hours later, γH2AX was quantified by flow cytometry. (C) HeLa cells were infected or treated with etoposide, then embedded in agarose, lysed, and subjected to an electric field in neutral condition that allowed migration of broken DNA out of nuclei (comet assay). DNA was stained and examined by fluorescence microscopy. (D) Cells were infected as in (B), the comet assay was performed, and the mean comet tail moment (product of tail length and fraction of DNA in the tail) was measured. The error bars represent the standard error of the mean.
E. coli strains harboring a genomic island, widely distributed in both pathogenic and commensal isolates, induce DSBs upon transient contact with epithelial cells (fig. S7). This genomic island is present in Nissle 1917, a commensal strain of E. coli that is an excellent colonizer in mice and humans and has been widely used as a probiotic treatment for intestinal disorders, such as ulcerative colitis and Crohn's disease (22–24). Slowing the renewal of the intestinal epithelium by blocking the cell cycle could be a bacterial strategy to prolong colonization of the intestinal epithelium, which in turn should have an impact on pathogenicity and commensalism (12). The amount of the genotoxin produced by different strains together with the location and duration of the contact with the target host cells may be critical to whether commensalism or pathogenicity is promoted. Because DNA DSBs can give rise to genomic instability (25), the occurrence of bacteria with the pks island may also constitute a predisposing factor for the development of intestinal cancer (26). Friend or foes, synthesis of a bioactive polyketide-peptide in E. coli, the workhorse organism for genetic engineering, should facilitate progress in engineering hybrid peptide-polyketide biosynthetic pathways for making natural products such as anticancer agents, antibiotics, and immunosuppressants (27, 28).
Supporting Online Material
www.sciencemag.org/cgi/content/full/313/5788/848/DC1
Figs. S1 to S7
Tables S1 to S3
J. B. Kaper, J. P. Nataro, H. L. T. Mobley, Nat. Rev. Microbiol. 2, 123 (2004).
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M. Lara-Tejero, J. E. Galan, Science 290, 354 (2000).
O. Marches et al., Mol. Microbiol. 50, 1553 (2003).
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R. A. Welch et al., Proc. Natl. Acad. Sci. U.S.A. 99, 17020 (2002).
F. L. Nowrouzian, A. E. Wold, I. Adlerberth, J. Infect. Dis. 191, 1078 (2005).
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C. R. Hutchinson, Proc. Natl. Acad. Sci. U.S.A. 100, 3010 (2003).
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A. Sancar, L. A. Lindsey-Boltz, K. Unsal-Kacmaz, S. Linn, Annu. Rev. Biochem. 73, 39 (2004).
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E. P. Rogakou, D. R. Pilch, A. H. Orr, V. S. Ivanova, W. M. Bonner, J. Biol. Chem. 273, 5858 (1998).
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H. A. Malchow, J. Clin. Gastroenterol. 25, 653 (1997).
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We thank E. Helloin for experimental support and K. Sankaran, S. Tucker, and J. Piel for insights. This work was supported by European Union grant QLK2-CT-2002-00944, the German Research Foundation (Sonderforschungsbereich 479, TP A1, and International Graduate College 587/2), and the Competence Network Göttingen "Genome Research on Bacteria." The nucleotide sequence of the complete pks-island was submitted to the European Molecular Biology Laboratory (EMBL) database (accession number AM229678).
You are going to email the following Escherichia coli Induces DNA Double-Strand Breaks in Eukaryotic Cells
By Jean-Philippe Nougayrède, Stefan Homburg, Frédéric Taieb, Michèle Boury, Elzbieta Brzuszkiewicz, Gerhard Gottschalk, Carmen Buchrieser, Jörg Hacker, Ulrich Dobrindt, Eric Oswald
Science 11 Aug 2006 : 848-851
Microbes that normally live in the gut produce a small molecule that slows the turnover of the gut lining by damaging host DNA, possibly enhancing their colonization.

redpajama_set_name: RedPajamaCommonCrawl


The IRS released the 2018 required contribution percentage for the "affordability" threshold under the Affordable Care Act (ACA). The percentage will decrease to 9.56% from 9.69% in 2017. As a reminder, under the ACA's Employer Shared Responsibility provision – which is still applicable as of now - "applicable large employers" (generally, those with 50+ employees) must offer an affordable, minimum value health plan to their full-time employees and child dependents or be subject to a §4980H penalty for each full-time employee who receives a subsidy to purchase health insurance through the Marketplace / Exchange.
Update on ACA repeal: Senators are busy reviewing the ACA repeal bill "Better Care Reconciliation Act of 2017" released this morning. The bill will be scored by the Congressional Budget Office to estimate its impact on the budget and insurance premiums as well as the number of insured individuals. Senate Majority Leader Mitch McConnell plans to hold a vote next week and hopes to get the 50 votes needed to pass it.
The discussion draft of the bill, which is expected to change prior to the vote, appears similar to the American Health Care Act (AHCA) passed by the House of Representatives in May.
WHAT THIS MEANS TO EMPLOYERS: Employers who are subject to the ACA's Employer Shared Responsibility provision and who want to avoid exposure to penalties for not offering an affordable, minimum value health plan should review their employee contribution strategy to ensure that the total employee cost for enrollment in single coverage is no more than 9.56% of income for 2018. In order to meet this requirement, some employers will have to decrease the cost to employees for next year.
All employers should stay abreast of the progress of ACA repeal and replace measures. Until actual changes are made to the law, employers must maintain compliance with current rules to avoid potential adverse consequences.


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HÀ NỘI – Seeing share prices tumbling, some companies have decided to buy back their shares in an effort to increase their value.
Lam Sơn Sugar Joint Stock Corporation (LSS) decided to repurchase six million of the company's shares, equivalent to 8.6 per cent of outstanding shares, on the HCM Stock Exchange, after its shares lost over 33 per cent of their value in the last four months.
The transactions are expected to happen from December 1-31.
LSS shares have fallen below the par value of VNĐ10,000 a share since early this month, a loss of 33.2 per cent from the peak of VNĐ15,000 on June 26, and a loss of 15 per cent since the start of 2017. At this price, the company will likely spend about VNĐ54 billion for the repurchase.
The performance of sugar makers has slid this year after a strong showing in 2016. Revenues of Lam Sơn Sugar declined 53 per cent year-on-year to VNĐ214 billion (US$9.4 million) in the first quarter of the fiscal year 2017-18. Its net profit, at just over VNĐ12 billion, was just one third of its profits over the same period in 2016.
In 2016, the company's revenues and pre-tax profits increased 18 per cent and 38 per cent, respectively.
The poor result could be blamed on falling sugar prices on the world market, currently being traded around $15 per pound of sugar, down sharply from $24 per pound at the end of September 2016.
Another sugar company, Thành Thành Công Tây Ninh JSC (SBT) is also considering a repurchase of 83.5 million shares, or 15 per cent of outstanding shares, after its shares plummeted 50 per cent in the last three months, from over VNĐ41,000 per share in early August to just over VNĐ21,000 on Monday.
The company will seek shareholders' approval for the repurchase programme, which is expected to take place during daily trading on the stock exchange in the shareholders' meeting on November 20.
Earlier, the company's trade union registered to buy five million shares of the company for long-term investment purposes from November 15 to December 14.
After the merger with Biên Hòa Sugar (BHS) on September 6 this year, the total assets of SBT increased from VNĐ7.8 trillion to VNĐ19.4 trillion, of which equity capital is more than VNĐ6.9 trillion and liabilities were nearly VNĐ12.5 trillion. The company holds about 30 per cent of the domestic sugar market share.
Besides sugar firms, garment manufacturer Everpia JSC (EVE) has also announced a repurchase programme of three million shares, equivalent to 7.1 per cent of its capital. The company's shares have lost about 32 per cent of their value this year after it reported gloomy business results in the first nine months: the company posted VNĐ79.7 billion in net revenues and VNĐ32.4 billion in net profit, down 62 per cent and 50 per cent year-on-year, respectively.
Hoàng Quân Consulting-Trading-Service Real Estate Corporation (HQC) has decided to buy back a maximum of 10 million shares, equal to 2 per cent of outstanding shares, to maintain the share value after it plummeted 50 per cent this year to around VNĐ2,600 per share.


IF I'M UNDER 18 WILL I BE ALLOWED ENTRY TO CROSS THE TRACKS?
Children of all ages are permitted. All children and young persons up to and including the age of 17 must be accompanied by an adult over the age of 26. This MUST be the parent, legal guardian or nominated guardian of the young person. An adult over the age of 26 can accompany a maximum of 4 young persons.
Babies and children under 10 do not need a ticket, so enter free of charge.
Children aged 10 and over require their own ticket charged at 50% of the adult ticket price.
The adult ticket price will apply from the age of 18.
We accept passports, UK driver's licenses or provisional licenses.
WHEN DOES CROSS THE TRACKS TAKE PLACE? WHAT ARE THE TIMINGS?
Cross The Tracks will be held on Sunday the 9th of June 2019. Gates open at 12pm midday to Brockwell Park, the event will run to around 10.00pm (subject to license).
Last entry to the event is 8pm.
Tent. They will also be allocated at each toilet block.
will offer an alternative serving policy if a lowered counter is not made available.
Please ask our staff for help.
limited and is within picnic areas only.
stages. Lasers may be used for some performances.
ARE THE SET TIMES AVAILABLE?
Set times for the Brockwell Park will be available nearer the time of the event. Please check our social networks for updates.
WHERE CAN I BUY A CROSS THE TRACKS TICKET?
IF I LEAVE CROSS THE TRACKS, WILL I BE ABLE TO GET BACK IN?
No, there's no re-entry. The same applies to our night events..
ON THE DAY WHERE CAN I COLLECT MY LOST PROPERTY?
Lost property will be clearly labelled on site. Please ask the staff there for more information..
ARE THERE ANY LOCKERS ON SITE?
There are no lockers on site, please try to avoid bringing large bags or any other item which may need storing whenever possible.
WILL I BE ABLE TO BRING MY OWN FOOD AND DRINK TO CROSS THE TRACKS?
Not once you've reached the search teams, after then you cannot take food and drink onto the site – apart from one sealed plastic bottle of water. Local food and drink vendors will be available on site. No food and drink may be brought to the late night venues..
ARE DOGS ALLOWED INTO THE EVENT?
No pets are allowed on site. Assistance dogs will be permitted.
DISABILITY ACCESS DOES CROSS THE TRACKS HAVE DISABLED ACCESS?
All disabled customers are welcome at Cross The Tracks. There are a number of facilities on site to accommodate disabled customer's needs, these vary from venue to venue. Not all disabled customer's need use of these facilities but we will endeavour to assist with access and provide a free carer/PA ticket where required to ensure your stay is comfortable and most of all enjoyable.
I'D LIKE TO TRADE AT CROSS THE TRACKS, WHO CAN I CONTACT?
Please email info@xthetracks.com for more information.


text:
Live Gig Video: Glass Animals perform mega hit 'Gooey' on David Letterman
By Mary Chang on Friday, 27th February 2015 at 4:00 pm
The speed of Glass Animals' train doesn't show any signs of slowing down. After sufficiently creeping us out last week with the new promo for 'Black Mambo' and the Oxford band's most recent UK live appearance last Sunday at the 6Music Festival 2015 in Newcastle, they jetted back to the States.
In New York City, they played a one-off intimate show for fans on Tuesday night at the Box in the Lower East Side, which they then followed with this performance of their mega hit 'Gooey' on the venerated Ed Sullivan Theater stage for Late Night with David Letterman Wednesday. They brought their full jungle kit, which naturally host Dave couldn't help but joke with the band's Dave about needing some ferns for his digs. Watch it below.
Glass Animals' next UK tour begins Monday in Cambridge.
https://www.youtube.com/watch?v=Z1cycNRMS2o
Live Gig Video: Glass Animals perform their hit single 'Gooey' at Capitol Studios in Los Angeles
Live Gig Video: Bob Dylan performs 'The Night We Called It a Day' on penultimate Late Show with David Letterman
Live Gig Video: Daughter perform 'Youth' for David Letterman
Live Gig Video: Divine Fits perform 'Baby Got Worse' for David Letterman
Live Gig Video: Holy Ghost! perform 'Dumb Disco Ideas' on David Letterman
Tags: 2015, davidletterman, february2015, glassanimals, live, newyork, video


A well-made puttanesca sauce can be one of the most intensely flavorful of all the types of sauces in Italian cuisine. The recipe I have for you today is quick, inexpensive, and contains easy to find ingredients. The combination of olives, capers, garlic, hot pepper flakes and anchovies (don't panic! See below for more on this critical ingredient), makes for an unforgettable sauce.
Best of all, you'll easily be able to make this dish in 20-25 minutes from start to finish.
Of course this dish takes its name from uh, ladies of the night. Depending on whose history you prefer, either this was a dish prostitutes would offer to entice customers into brothels, or it was an easy and cheap meal that prostitutes could prepare between customers. Let's just leave that subject right there, shall we? Ever since my inappropriate discussion on stiffly beaten egg whites, I've been trying to keep this blog more G-rated.
The entire dish should cost around $7-8 to make and it serves 5-6 easily. That may not quite qualify for laughably cheap, but it's pretty close. Furthermore, this is a dish that scales quite easily, so you can certainly double the recipe for larger dinner groups.
One final comment on anchovies. If you don't like them, fear not. Laura can't even sit in the same room with an anchovy, yet she absolutely loves this dish. Do not leave them out.
Then, add each of the other ingredients one by one as the sauce comes to a boil.
Once you've added all the ingredients and the sauce is beginning to boil, turn down the heat to medium low. Let the sauce simmer and thicken for 10-15 minutes, stirring occasionally, while you cook the pasta.
Ladle sauce liberally onto pasta and serve immediately!
First, let's take a quick look at a photo of all the ingredients you'll need.
Notice the seemingly extraneous glass of wine. Although this recipe doesn't include wine as a specific ingredient, we have seen before that a glass of wine serves critical functions for the chef, both as an analgesic and a mood-lifter.
Use the spatula to break up the tomatoes into coarse chunks. You'll probably want to at least quarter every tomato, even the smaller ones. Proceed carefully with this part of the recipe, and do not wear white.
I usually pour the capers into a small strainer and then run cold water over theme to rinse them. After that, you can drop the little guys straight into the pan.
Add each of the other ingredients one by one. In goes the garlic!
If you either loathe or fear anchovies, or if they gross you out in any way, skip the next two pictures. But DO NOT LEAVE THEM OUT OF THIS RECIPE. They are an absolutely critical element of the overall flavor of the sauce. Remember, even though Laura claims she cannot sit in the same room with an anchovy, she still loves this dish.
Chop up the anchovies into smallish pieces, maybe the size of an aspirin tablet or smaller. You'll want them to disintegrate in the boiling sauce.
Ladle onto your pasta. A wide/flat pasta like linguine or fettucine works well for this dish, but honestly any type of pasta is fine.
Literacy alert! I'll give away a free gift subscription to Casual Kitchen for any person who leaves a comment naming the author and poem that's excerpted in the picture above. :) Good luck!
Looks like a great recipe, by the way.
First you liberated the lowly beet from the dirt-floor of the Slavic cellar; now you have raised from the depths the anchovy and brought it to it's rightful status as mini wonder-food, the miracle of Amalfi, the fishy flavor-bomb, a sort of natural MSG from the ocean.
I often slip anchovies in to sauces and braised dishes and have sometimes had to hide the can so as not to turn off some who know not what they fear.
I usually add half to the dish and eat the other half from the can on bread, washed down with a beer. this is only for those who "really" love anchovies.
I have noticed in the past few years that fresh anchovies have made a comeback on some menus, particular at spanish tapas and italian restaurants. When they are fresh they are stunningly good.
For a more traditional Napolitan or Amalfitan anchovy experience, order an anchovy pizza at Patsy's in East harlem or Pepe's in New Haven.
So true about people "knowing not what they fear." So true.
And I've been known to sneak a few anchovies myself.
Just made this tonight and it's spectacular! I didn't know what I'd do with the remainder anchovies, so minced them fine and threw them all in. They added an elusive and savory flavor, but I couldn't see them in the finished sauce.
I'm trying not to go into the kitchen and drink the rest of the sauce.
Thanks for your comment Alphacyg! I've been tempted to drink this sauce on occasion too. It is that good. Thanks for reading!
never used a caper before, are you supposed to cut them in anyway or just chuck 'em in whole?
Mr.Physix, Just chuck 'em in whole. At least that´s what I do. No need to cut them up.
This looks so good! We're not big fans of large cooked tomato hunks in things so I will probably use diced tomatoes, but I'm definitely going to try it soon. I've always wanted to make puttanesca.
Great recipe! Puttanesca sauce is a favorite of mine and I will definitely bookmark this page...I love this colorful, chunky, tasty sauce on my pasta.
Kaija, interesting and creative ingredient substitution idea! Thanks for sharing.


Five internees, with two crouched near the snow-covered ground and in the act of throwing large snowballs. Men are posed in front of a building with one framed window visible. Building seems to be made from studded wood planks. Nello Trasciatti is standing in the middle in the back.
Internees were not allowed to have their own cameras in camp. However, it seems that Giacomelli or one of his campmates were able to secure such a camera, as this is clearly an informal photograph. Given the setting and subject, it was unlikley that the official government photographer was involved.
The location cannot be confirmed; it is likely either Fredericton Internment Camp or Camp Petawawa. Most of the other pictures in this collection were taken at Fredericton Internment Camp in Fall 1942 or Winter 1942/1943.
Although recreational activities were organized during downtime, internees were often lonely and bored.
NOTE: there is a duplicate print in the collection (DICEA2011.0001.0020).


Custom new home design builders are for people who want to live in a particular location like Alawa other than buying a pre-made building. Strategies and plans are created by the home designers, builders and architects according to the home owner's choice such as the building design layouts, location, size and budget.
The home buyers or land owners hire new custom home builders to construct a house on an already bought building space. All types of custom design building services are available at the companies in Alawa. Custom design buildings last longer than the pre-constructed buildings due to the quality of building materials used in them and attention to detail.
Custom design buildings are unique and can be made according to the building owner's requirements. An architect can become handy in designing the house blueprint before construction. Custom design building companies found in Alawa have their own architects and builders so you don't need to hire any extra labour or concern yourself with the council approval process. All this should be a part of a professional builders system..
Custom design buildings are designed and constructed according to the building owner's choice, so, if you're interested in having a custom building service, the materials, paints, decor, etc. will all be up to you.
When making decisions of your house construction, you are literally helping the builders and designers in their work.
You can choose the appliances, such as air conditioners and heaters accordingly.
With custom design building services, you are able to build your dream house your way.
Custom design building services are also great for office buildings. You can design your new office building and have it constructed under your budget and personal requirements.
The Alawa building constructors offer valid plans and strategies for you to choose from.
You can get help from the financial advisor's regarding your plans, ideas and building budget.
Basically you as the upcoming owner are in full and absolute control..
When choosing your Custom design builder for you new home it is important to note some important points.
Gives your Alawa home a modern feeling and keeps it competitive to the current house engineering innovations.
The first step in owning a home is building it, and the next important aspect of being a home owner is keeping the homes condition at its best from the day that you have built your custom designed dream house.
With a luxurious home you need a qualified Alawa professional expert trades person to help with bathroom maintenance, indoors, windows repairs, and general yard maintenance. This will give you peace of mind and make sure that your home is always at its very best condition, year in and year out.


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Spokane City Council hires Brian McClatchey as legal adviser
Sat., April 4, 2015
By Nicholas Deshais nickd@spokesman.com(509) 459-5440
An ally of Spokane City Council President Ben Stuckart has been chosen to replace city attorney Mike Piccolo as the council's legal adviser, a move designed to assert the council's independence from Mayor David Condon's administration.
Brian McClatchey, a local attorney with ties to regional tribes and experience on the city's Plan Commission, was picked by a unanimous vote of council members Thursday afternoon.
Councilman Mike Fagan, who voted to approve McClatchey's hiring, said Friday that he would have preferred hiring another finalist for the position, Jo Ellen Thomas, over McClatchey, saying her "qualifications and experience blow Mr. McClatchey out of the water. They do, truly."
Fagan, the most conservative member of the council, said he supported McClatchey's hiring instead, "acknowledging the fact that I'm going to have to work with him for the next year. I don't want to start out on a bad note with him. But am I going to get a fair shake in the future? I don't know. Only time will tell."
McClatchey said he looked forward to his role in "providing information to every single council member to help get their legislative priorities to reality."
"I will treat every council member as a client and provide my best efforts to each and every one of them," he said. "I look forward to forming a sound relationship with Mike Fagan. I enjoy working with him. I think he brings a refreshing voice."
McClatchey has donated nearly $3,000 to liberal and Democratic candidates and causes in recent years, though not to Stuckart. In November, when Stuckart became the subject of an ethics complaint for improperly sharing an email dealing with a confidential lawsuit, Stuckart said he had hired McClatchey to represent him.
At the time, McClatchey called the ethics complaint a "distraction."
The ethics committee ruled that Stuckart had violated the city's ethics code, but could not find any evidence that his actions hurt the city. He was fined $250.
At Thursday's meeting, Stuckart said he and McClatchey had ultimately decided not to work together on the ethics complaint. McClatchey echoed Stuckart.
"I decided that there might be an appearance of conflict so I withdrew and I didn't represent Ben in front of the ethics committee," McClatchey said.
When McClatchey begins on April 20, he will perform most of the roles of a city attorney, including legal research and analysis, drafting ordinances and resolutions, and attending council meetings. He will not represent the council in court, because of restrictions in the city charter. McClatchey's position as policy adviser will come with an $87,000 salary, which was funded by the council when it approved this year's budget in November.
As policy adviser, McClatchey will be an at-will, exempt employee, meaning he won't be part of a union. Due to an ordinance the council passed this year, votes from five council members are needed to remove McClatchey. Piccolo, whom he is replacing, will remain with the city attorney's office.
"It's an amazing opportunity to be the first person to have this job. It's something the council needs," McClatchey said. "They need the right staffing in place to help them be as smart as they possibly can. It's not about us versus them."
Under Stuckart's leadership, the council has said strengthening its autonomy was a goal.
"We've said it since 2012. We were all really clear we wanted to assert the independence of this branch of our government. This is one step in that direction," Stuckart said. "If there's disagreement on a piece of legislation, where the mayor opposes it and the council is moving it forward, there needs to be a clear delineation on the legal end."
Aside from numerous council assistants, the council office employs a budget analyst, Debra Robole, but she was hired by the council before Stuckart was elected.
Councilman Mike Allen, who was absent during Thursday's vote, said McClatchey was his "first choice."
"I always found him to be very professional and courteous," Allen said, noting that he believed McClatchey would offer fair advice to all members. "With any of these jobs, if his performance is biased, then that will definitely present itself."
Stuckart agreed, but suggested that politics are a part of City Hall.
"It was a unanimous decision. That speaks highly of Brian," he said. "Nobody questions Theresa Sanders' ability to be a great, strong city administrator. And she donated to the mayor in 2011 and maxed out this time. But nobody questions her advice."
Sanders, who holds one of the most influential positions at City Hall, has donated $1,800 to Condon's re-election campaign, to his 2011 campaign, and to other conservative or Republican candidates.
McClatchey, a graduate of the University of Michigan Law School, recently practiced law with K&L Gates and was the in-house attorney for the Coeur d'Alene Casino from 2007 to 2012. He has owned his own law firm since last summer and is the vice president of the city's Plan Commission.
He also chairs the commission's subcommittee on transportation, a new board tasked with determining how the 2014 street levy money will be spent during the next 20 years. With his new position, McClatchey must resign from those roles at the city.
Published: April 4, 2015, midnight
Tags: ben stuckart, brian mcclatchey, coeur d'alene casino, david condon, mike allen, mike fagan, mike piccolo, plan commission, spokane city hall, theresa sanders


text:
Q: Problem on JSON date Parsing I have a JSON source that sends its data that I display on my web page.  Problem is, the
Date part that it is returning is some sort of a javascript object.
Please look at the sample response.
dteInstallDate
 date   16
 day    4
 hours   0
 minutes   0
 month   8
 nanos   0
 seconds   0
 time   1284566400000
 timezoneOffset -480
 year   110

When I try to print the object as console.log.  It prints
data.resource_data.dteInstallDate.toString()
[object Object]

My problem is that I want to set this JSON response into my Jquery hidden input it updates the data into Object Object.
$("div#pmItems").find("#dteInstallDate").val(data.resource_data.dteInstallDate);

I am having thoughts on how to send Date object from Server Side into Javascript.  Should I just convert it to string or send it as a long value.  Which one do you think is better?
I have Spring MVC as my backend

A: You have a JSON source, correct? JSON is simply a notational standard for the purpose of cross-language communication. While a JSON object has the same notation as a shorthand-declared Javascript object, it is really just a representation of any object in any language. If you were to get a JSON feed that looked like this:
{
    "dteInstallDate":
    {
        "date"      : 16,
        "day"       : 4,
        "hours"     : 0,
        "month"     : 8,
        "nanos"     : 0,
        "seconds"   : 0,
        "time"      : 1284566400000,
        "timezoneOffset": -480,
        "year"      : 110
    }
}

In Javascript, all you'd have to do is call up the dteInstallDate.time property and put it into a new Date() object. A working example is here:
http://jsfiddle.net/kAxfY/
As you can see, the time is a little off what they say...I tried fooling around with the timezoneOffset to make it more accurate, but I couldn't. Typically, timezone offsets are done in minutes, so -480 would mean that it's -480/60 = -8 hours behind GMT. For more info on the Javascript Date object, see this page:
http://www.w3schools.com/jsref/jsref_obj_date.asp
Edit: and perhaps a bit more toward the point of your post...it doesn't matter how you send this data across to the browser, as long as enough information is there to create a Date object in Javascript. The four options for creating the date object are:
//no argument means it will create a date representing the local time on the machine on which the browser is running
var d = new Date();

//milliseconds
var d = new Date(1284566400000);

//a string
var d = new Date("October 13, 1975 11:13:00");

//giving precise values for each unit of time
var d = new Date(year, month, day, hours, minutes, seconds, milliseconds);


: : I am interested in finding out about the Reynolds family in Mohill. My great-grandmother was named Brigid Reynolds from Mohill. She emigrated to the US at the time of the potato famine, after marrying Michael Early. I would love to find out more information, especially about surviving relatives. Thank you!
: Joan, I am not sure if we have corresponded before. My Stanfords and Carrolls are from Mohill and settled in New Haven, CT in 1847. In doing research on these families in both Mohill and New Haven, I have come across many Reynolds and Early's. I will go over my notes and databases and let you know what I have. Most of the names I have would have some connection to a Stanford or a Carroll.
My mother was a Reynolds from Mohill her Town land was Lisomadaun. We are very close i live in Wallingford. Are a Member of the New Haven Gaelic Club.


The Buñol Castle is made up of two enclosures located on a rocky ridge between the Buñol river ditch and the Borrunes ravine, separated by artificial pits that are saved by stone bridges, defended by towers that function as doors. The first enclosure, called "North enclosure", is a straight polygon flanked by two towers in the corners further north and a central tower that serves to defend the door. The perimeter is crossed by a wall in which there are semi-detached houses from the 20th century. On the opposite side to the entrance tower, there is a door through which the bridge that crosses the central moat is accessed. Through a tunnel made at the base of the Homage Tower, you access the second site, which preserves the residential buildings of the fortress.
INTERIOR FEATURES: Arches, open interiors, staircase, tiled floors.
EXTERIOR FEATURES: Arches, balconies, bridges, cobblestones, doors with nails, metal fences, fountains, gates, porch, patio.


Once you have installed and activated the plugin, each widget added to a sidebar in Dashboard>>Appearance>>Widgets attains a further set of options, which will show or hide the widget; To all users, to logged in users only or to logged out users and on specific pages or posts.
Using Display Widgets SEO Plus can be a little complex if you have a large number of widgets you wish to use, the default is that a widget will be displayed on all pages and for all users, but you can choose to either; Hide a widget on all pages except those selected, or to display a widget on all pages except those selected.
Display Widgets SEO Plus has a very nice feature whereby if you please and "!" at the beginning of the widget title, the widget title will not be displayed at the front end but is displayed at the back end. This is useful when there's no need to display a title but you need one to keep track of your widgets in widget configuration page.
Display Widgets SEO Plus also supports BuddyPress very well, allowing widgets to display on the various BuddyPress pages, the individual group pages, forums, activity etc. This feature is at the top level for pages, you can select for example all Group pages and this will display the widget on the sidebar of all individual groups, what you can do is only display a widget in the sidebar of group "X". The developer hopes to add that feature later.
Overall I give this plugin a score of 9 out of 10. I think that it can be overly complex but it supports BuddyPress very welland gives a great deal of control over where and to whom a widget can be displayed.


text:
3989 Odin eller 1986 RM är en asteroid i huvudbältet som upptäcktes den 8 september 1986 av den danska astronomen Poul Jensen vid Brorfelde-observatoriet. Den är uppkallad efter den fornnordiska guden Oden.

Asteroiden har en diameter på ungefär 3 kilometer.

Referenser

Huvudbältesasteroider
Småplaneter namngivna efter mytologiska figurer
Astronomiska upptäckter av P Jensen
Astronomiska upptäckter 1986

redpajama_set_name: RedPajamaWikipedia


text:
//
//  LinkedListsDetectaCycle.cpp
//  HackerRank
//
//  Created by Fabrizio Duroni on 01/11/2016.
//
//  https://www.hackerrank.com/challenges/ctci-linked-list-cycle

#include <iostream>
#include <string>

using namespace std;

/*!
 C alike struct definition.
 The attribute Node is be declared with struct
 because in C, Node is not yet defined at this point.
 */
struct Node {

    int data;
    struct Node* next;
};

/*!
 Detect a cycle in a linked list.
 Detection is done using Floyd's cycle algorithm.

 @param head the head of the linked list.
 */
bool has_cycle(Node* head) {

    Node* slowNode = head;
    Node* fastNode = head;

    while (slowNode != nullptr && fastNode != nullptr && fastNode->next != nullptr) {

        slowNode = slowNode->next;
        fastNode = fastNode->next->next;

        if (slowNode == fastNode) {

            return true;
        }
    }

    return false;
}


text:
Appleby-in-Westmorland – miasto i civil parish w Wielkiej Brytanii, w zachodniej Anglii, w hrabstwie Kumbria, w dystrykcie Eden. Położone jest nad rzeką Eden, w połowie drogi między Penrith i Brough. Miasto do roku 1974 nazywało się Appleby nazwę zmieniono, by zachować ciągłość historyczną. W 2011 roku civil parish liczyła 3048 mieszkańców.

Historia
Miasto założone przez Normanów. Rozwinęło się dopiero w XVIII wieku za sprawą targu koni, który odbywał się corocznie aż do roku 1974. Pierwszy koński targ miał miejsce w roku 1685, a pozwolenie wydał król Jakub II. Obecnie restytuowany i jest jednym z największych tego typu wydarzeń w Europie, choć nie jest to impreza oficjalna.

Przypisy

Miasta w Kumbrii
Civil parishes w Kumbrii
Eden (dystrykt)


text:
Home › Culture › Old Textile Mill Won the City of Oslo Architecture Award
Old Textile Mill Won the City of Oslo Architecture Award
By ThorNews on October 17, 2013 • ( 0 )
On Wednesday, the City of Oslo Architecture Award was awarded in Oslo City Hall and the old textile mill in Nedre gate 5-7 won this year's award. The project is carried out by architects from Space Group Arkitekter AS and landscape architects from Sundt & Thomassen AS.
Nedre gate 5-7 is located in a listed industrial area by the Akers River. The building that was a textile mill from the 1890s until 1933 is now adapted to a wide range of cultural activities, which the jury appreciated.
The project has preserved historic features including roof and brick walls. The building is characterized by innovative architecture with open mezzanine floors, large windows providing ample light and a sculptural staircase.
(article continues)
– In dialogue with conservation authorities, the endangered textile mill from the 1890s has been further developed and given new life with a wide range of cultural activities. The project demonstrates innovation regarding conservation issues and architectural and building design solutions. In a discreet and exemplary way, past and present is linked together – for the future, the jury wrote.
The aim of the award is to highlight a unique building built in Oslo in 2012.
This year there were 22 candidates. Nedre gate 5-7 was named one of three finalists, along with Enebolig Sømme and Tjuvholmen Icon Complex, better known as the Astrup Fearnley Museum.
The City of Oslo Architecture Award will go to a building that represents innovative architecture or landscape architecture with high conceptual quality and execution, and that brings something new – aesthetically, functionally or culturally.
Source: osloby.no
Photos by: Space Group Arkitekter AS
‹ Norway's new Minister of Culture and Church Affairs: Thorhild Widvey
Norwegian Apple Day – October 17th ›
Categories: Culture, Design


BUSINESS PLAN FOR THE EXPANSION OF NIGHTINGALE MEDICAL CENTRE IN KISUMU COUNTY PROPOSED BUSINESS COMMENCEMENT PERIOD: From January 2014 May 2013 CONTACT DETAILS: Dr Philip Chek Nightingale Medical Centre P... A Sample Convenience Store Business Plan Template Convenience Store Business Overview One of the easy to enter and thriving business line that an entrepreneur how is looking towards starting a business of his or her own is to go into retailing business.
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business plan for bookstore cafe Topic of the manual is about the greatest of these business plan for bookstore cafe can have lots a large number of different products represented.
A Sample Convenience Store Business Plan Template Convenience Store Business Overview One of the easy to enter and thriving business line that an entrepreneur how is looking towards starting a business of his or her own is to go into retailing business.
Green Office office supplies retail business plan company summary. Green Office is a start-up company offering a wide range of office supplies, all of which are environmentally friendly. Green Office is a start-up company offering a wide range of office supplies, all of which are environmentally friendly.


Save 10% on a new custom deck from HAL CO when you submit your request today!
Building custom decks can have a huge impact on the look and feel your Gainesville, VA home. This is because of the endless possibilities that they generate. No matter what type of home you own, custom decks can help to give them that new edge. Building custom decks is essentially a window of endless possibilities in Gainesville, VA. You can choose any material that you like, such as wood, metal, or composite deck materials, and then go from there. Any shape or size that you want is an option, and you can even further customize custom decks to meet any other particular needs that you may have.
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* Fils de Dieu goes on like a cocktail (possible a mojito) with citrus, coconut, ginger, and green notes blending together.
* As it develops, it smells even more like a mojito with lime and vanilla joining in as the coconut note increases in intensity.
* This wears differently on me sometimes. It's gone in a floral direction a few times with rose and jasmine coming up. Other times the mojito gets dosed with a foody notes of cinnamon, cardamom, and rice.
* The mojito drifts away as vanilla and amber form the base with a soft leather and musk chiming in.
Summary: When I was in Paris last year at Eldo boutique in the Marais, I got to sniff this and it was referred to as Filipino Houseboy. I am guessing they renamed it because they were afraid that it might be considered offensive. Seeing as this is the same brand that released Secretions Magnifiques, arguably the most offensive fragrance ever made, I am unclear as to why they changed the name. I love this scent and plan to buy a bottle as it is light enough for summer wear as an Eau du cologne but has enough weight to stick around. Fils de Dieu could be considered office-friendly in light doses. If you spray on too much, it does start to project loudly. Longevity is strong, more than 8 hours.
Fils de Dieu is available at Lucky Scent , Min New York , Henri Bendel and other select boutiques.
This one is on my to-try list.
It's a great one. I was impressed by the longevity of it. I found out that if you go to the Paris boutique, they actually have a limited edition of bottles labeled as Philipino Houseboy.
Well, you know how I felt about the old name. Now the new name is too long to say.
Tried this at Scent Bar this week. I want a bottle badly.
I am totally with you. I found someone in Paris who is going to buy me a bottle of Philipino Houseboy.. because that so fits with me decor… don't you think?
At least I can remember the name PH. I can't remember what it is called here.


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