text:
Russia Warns US Of Response If Its Property Is Not Returned
MOSCOW (AP) — Russia has issued a new warning to the United States that it might expel U.S. diplomats or shut down its property in Russia if its diplomatic compounds in the U.S. are not reopened.
In response to reports of Russian meddling in the presidential election, the outgoing Obama administration last year expelled 35 Russian officials from the U.S. and shut down two Russian estates in the U.S. that President Barack Obama said were being used for spy operations.
The United States and Russia on Monday resumed high-level talks aimed at resolving irritants between Washington and Moscow.
In its readout of the meeting, the Russian Foreign Ministry said
Tuesday that the situation remains unresolved and that Moscow "reserves the right to reciprocal measures" if the Russian property is not returned.
(First reported by AP News and retrieved from Townhall News)https://townhall.com/news/politics-elections/2017/07/18/russia-warns-us-of-response-if-its-property-is-not-returned-n2356408 (July 18, 2017)
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Vaccines chief is doing a good job, but we need transparency
By Philippa May @HTnewsroom Entertainment Editor
Jesse Norman's wife Kate Bingham, chair of the Vaccines Task Force
Kate Bingham, the head of the Government's Vaccines Task Force, seems to be doing good job securing vaccine supplies for our country, and is laudably doing so without pay.
The issue is not with Kate (wife of Herefordshire MP Jesse Norman) as an individual, but with the Government and its processes for appointments and commissioning of services and supplies.
There is a network of Tory-linked appointments and companies around testing, vaccine procurement and PPE purchasing, of which Dido Harding (head of track and trace and wife of John Penrose MP) is the best known example.
The Guardian has dubbed this the 'rise of the Chumocracy''. Making appointments without always going through normal processes allows suspicion to arise.
Awarding contracts without competitive tendering has resulted in the National Audit Office criticising cabinet office procedures in a number of cases.
£21 million has been paid to a middle man to liaise with a jewellery designer for PPE.
Vast sums of money are being spent on our behalf with private providers, often with little or no track record of working with the NHS, without adequate scrutiny.
This was understandable at the start of the crisis in February and March, but eight months on it is important that proper processes are in place to prevent waste and the appearance of cronyism.
Anna Coda

redpajama_set_name: RedPajamaCommonCrawl


INTERACT (Interventions to Reduce Acute Care Transfers) is a quality improvement program that focuses on the management of acute changes in a resident's condition in order to stabilize the patient and avoid transfer to an acute care facility. This includes providing the Skilled Nursing Facilities (SNF) with clinical and educational tools and strategies for use in every day practice in long-term care facilities.
Reduce avoidable emergency department visits within the Medicaid/ dual eligible nursing home population by 25% by the year 2020.
To incentivize facilities to use various INTERACT tools.
Provide Skilled Nursing Facilities (SNF) with the tools, workforce, and IT support needed to provide the best quality care for their residents.
Eligibility is open to any Skilled Nursing Facility (SNF) within Care Compass Network's nine-county region.


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NXT BLD
DEVELOP3D
Featured Opinion Structural engineering
Understanding embodied carbon in buildings
Posted on November 30, 2021 at November 30, 2021 by Stuart Campbell
Identifying and calculating the embodied carbon in proposed structural designs is increasingly an area where technology can take the strain, leaving engineers free to take the decisions and actions that will help reduce it.
As we edge closer to the 2050 Net Zero deadline and the world becomes more environmentally conscious, increasing attention is being paid to how the construction industry can build greener. Embodied carbon may be an important part of the sustainability puzzle.
The current spotlight on construction isn't without just cause; it's a shocking statistic that the built environment and construction currently accounts for around 40% of global energy-related CO2 emissions, according to the World Green Building Council (WGBC). As such, we are all under pressure to reduce carbon footprints and improve sustainability, from manufacturers and suppliers to contractors and engineers.
Already, we have seen a number of initiatives that aim to encourage change within the construction industry. For example, 2019 saw UK structural engineering consultancies declare a 'climate and biodiversity emergency'. In 2020, the UK government published The Construction Playbook, outlining green initiatives for the sector; and earlier that year, Institution of Civil Engineers president Rachel Skinner launched her 'Shaping Zero' campaign.
According to RIBA, clients in all sectors are increasingly commissioning whole life carbon (WLC) assessments as part of project requirements, driven by both environmental and economic considerations. We are also increasingly seeing more contractors instigate positive change too, with many of the UK's largest construction companies pledging targets around carbon emissions, including Willmott Dixon, Kier, Mace and Balfour Beatty.
Explaining embodied carbon
There are many factors that feed into this greener, more sustainable future of construction, with embodied carbon being just one of them. The embodied carbon of a building is defined as the greenhouse gas emissions (GHG) generated from the building's construction lifecycle. This can include carbon emissions associated with all materials, products and systems, from raw material extraction, manufacturing and transportation, the construction process itself and right through the building's entire lifespan.
Conversations around embodied carbon are becoming increasingly common, with clients and developers asking the right questions and showing a desire to build greener, whether they're driven by a 'moral' change in mindset or a genuine interest in understanding the environmental and carbon impact of their building
Embodied carbon assessments are not yet a legislative requirement, although that's not to say there won't be some form of regulation or carbon tax in the future. Either way, conversations around embodied carbon are becoming increasingly common, with clients and developers asking the right questions and showing a desire to build greener, whether they're driven by a 'moral' change in mindset or a genuine interest in understanding the environmental and carbon impact of their building.
With a significant portion of a building's final embodied carbon value often determined as early on in the construction sequence as the initial design stage, it's clear that the sooner action is taken, the better. With that in mind, carbon modelling and assessments can be invaluable, helping engineers and contractors to reduce the level of embodied carbon within their structures and, ultimately, build greener.
Of course, in order to design smarter and greener, you need to have an understanding of the carbon levels that you are dealing with from the outset. By calculating the embodied carbon present early on in the construction sequence, engineers and other project stakeholders can have a detailed understanding of the level of carbon that the proposed structure contains.
And thanks to advances in digital technology, it is also possible to take this further, drilling down deeper, measuring intelligently and providing a live overview of the carbon contained within a structure's individual components.
Having access to such detailed data early on can be invaluable, providing the information and, most importantly, the time savings necessary to consider, assess and evaluate how this carbon figure can be minimised, in order to reduce building's environmental impact.
Design optimisation
In many ways, the task of reducing a structure's embodied carbon boils down to design optimisation. This, of course, is a challenge that engineers already face on every project, and it's typically associated with decisions around materials and resources used, the type of foundation or column support grid chosen, and the delivery of the architect's brief – all without impacting overall structural stability. The same is true for carbon. In many ways, it's just one more factor to consider in this balancing act.
In other words, along with building strength, structural performance, design efficiency and cost, engineers must also take into account the amount of embodied carbon present. And to reach the most efficient and optimised design, they will need to compare different designs, materials and approaches.
Now engineers must balance the building's strength and structural performance with the amount of embodied carbon present too, as well as the design efficiency and cost. By comparing different designs, different materials, different approaches and calculating and contrasting the carbon within each design iteration, engineers can work to settle upon the most efficient and optimised design from all points of view.
Reducing waste
Of course, in addition to being smarter when it comes to embodied carbon, there are other things that can be done. First, rather than building new, we should look to reuse and repurpose existing buildings, structures and infrastructure where we can. Where that isn't feasible, we should work to design and build multiuse, multipurpose developments that are adaptable for the future, as well as reducing the amount of raw materials required for their construction. While this all stems from the efficiency of design, as already discussed, material wastage can be another big contributor to the carbon problem within construction.
Designed for use at the initial stages of design, Trimble's Embodied Carbon Calculator Tool enables engineers to automatically view, measure and understand the level of carbon included in their schemes
Here, it's all about the importance of accuracy and correct first-time fabrications and assemblies. We all know that an unresolved issue at the design stage of a project can have a snowball effect as the construction sequence progresses, leading to errors at the fabrication stage, the component not fitting correctly once it reaches site and then requiring subsequent rework or refabrication.
Incorrect quantities and over-ordering can be another issue, again leading to material wastage and unnecessary CO2 being pumped into the atmosphere. With a 3D digital environment, material wastage can be avoided. Thanks to the high levels of detail and visualisation offered by a 3D model, and the digital rehearsal that it offers, project teams are able to really see design issues and clashes and resolve any potential issues before a project progresses to the fabrication stage.
What's more, thanks to the data integration that BIM software supports, teams can benefit from the automatic generation of accurate material quantity take-offs, as well as data from the model automatically being transferred to the fabrication shop floor, minimising the potential for human error.
It's clear that there is much still to be done if we are to hit the Net Zero 2050 target and slow down the effects of global warming. As we continue to create the buildings and infrastructure of the future, working to reduce the embodied carbon they contain could make a big difference in the global fight against climate change. It's a big opportunity for the sector's engineers to make a noticeable positive impact.
Stuart Campbell is business development manager for engineering at Trimble (UK).
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Q: The type 'MaxLengthAttribute' exists in both 'EntityFramework, and 'System.ComponentModel.DataAnnotations I am doing some spring cleaning on a legacy Web Api 2 project (target 4.5).  As part of the the nuget updates, I moved Microsoft.AspNet.WebApi.HelpPage from version 5.0.0 to 5.2.3.  Once I do that, I am getting the following message:

Error CS0433  The type 'MaxLengthAttribute' exists in both
  'EntityFramework, Version=6.0.0.0, Culture=neutral,
  PublicKeyToken=b77a5c561934e089' and
  'System.ComponentModel.DataAnnotations, Version=4.0.0.0,
  Culture=neutral, PublicKeyToken=31bf3856ad364e35'

It looks like this was a problem a while back when they moved that class from EF to DataAnnotations and the solution was to upgrade EF.  Since I have the most recent stable version of EF (5.2.3), I am not sure what else to do.  I tried removing System.ComponentModel.DataAnnotations from the using statements, but then it doesn't pick up the EF MaxLengthAttribute.  Cleaning and rebuilding the solution also does not work.
Any pointers are appreciated.

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Home » Civil Servants » The Beginning of my journey and its end in the Department
The teacher I love, Mrs. Rashmi Saxena
मेरे प्यारे अध्यापक दीपक जी सर
The Great Teachers of My Life
Guarding from the Guardians
My experience with the Internet - good or bad?
Ode to Ms. Rutvi Mehta, my Social Studies teacher
Tribute to Prof. P. C. Mathur
The Beginning of my journey and its end in the Department
Somesh Arora
Somesh Arora is an Indian Revenue Service officer of the 1986 batch. He voluntarily retired in 2008 from the rank of Commissioner of Customs and Excise. He is now an advocate, journalist, tax author and columnist. He has a keen interest in astrology and poetry.
Every life has a different story with a purpose of its own. The jigsaw puzzle of life starts falling in place and is always better understood when in the later part of life one takes a hindsight view.
A small incident in life prompted me to come to the Department. It was in 1970's when I had just begun my college life.
One day I had a small throat ailment and had to visit a Homeopathic Doctor. I did not bunk my classes as I had two consecutive free periods. The clinic was run by a charitable hospital and was usually busy. Patients were accommodated by allotting numbers and had to wait for their turn.
The wait for me that day was rather becoming long as I had to attend the third period of an important subject, after the two free periods. While the wait was still on, a lady arrived along with two smartly turned up officers in uniform and was attended out of turn by the Doctor for about 15 to 20 minutes. The delay meant that I had to miss my class.
When my turn came I, being my usual expressive self, asked the Doctor as to why he had to break his own rules and accommodate people out of turn. Surprised at my query, the Doctor replied that the lady I was referring to was the Assistant Commissioner of Central Excise and was accommodated, as she was an important person.
The slight made me resolve to be the same important person as that lady was. Years later in 1981-82, I took my first shot at Indian Civil Services Exam in Amritsar, where only very few knew about Civil Services and there was hardly any guidance or coaching available. The first attempt itself took me to the interview stage.
While waiting for my turn at Dhaulpur House, a Clerk loudly called out my name. I packed up my files to be ready to go inside for the interview but the Clerk had other ideas. He loudly asked me if I knew anything about Civil Services? Surprised at his question, I asked him why he was asking. He replied that you are the only one at the interview stage who has filled up Indian Customs and Excise as the first preference. The whole hall had a hearty laugh. He convinced me and made me change my first preference to IAS, IFS etc. When the result eventually came, the attempt went waste despite my doing well in personality test.
Years later in 1986, I finally made it with a rank which allowed me to select IPS or Indian Customs and Central Excise Services. As the latter was the preferred one, I got into it and then did my probation for two years.
1986 batch of the Indian Revenue Service at the time of probation.
During probation we had occasion to be addressed by Shri J. P. Dutta, who was Chairman, C.B.E.C at that time.
Many of my batch mates, while undergoing training in Chennai, had to face ailments like typhoid as the acute water shortage and supply through tankers left us to drink some kind of contaminated water.
The story was no different at R.T.I., Bhandup in Bombay. Only this time, mosquitoes were taking us to task through malaria.
The vocal ones among the probationers decided to bring their grievances before the Chairman, driven by the desire to at least get a better deal for the forthcoming batches.
As soon as they started, they were disrupted by Mr. J. P. Dutta, who commented that while being taken in the service no one was promised conditions better than those in which the common citizens of India live; and that every one out of us had signed the dotted line on the agreement of service with full awareness of all terms and conditions. Therefore, it was expected of all the officers to accept whatever was offered or to exercise the right to quit rather than cribbing about service conditions.
Everyone became quiet after such a straight forward remark. Perhaps whatever he stated had a little deeper impact on me.
On getting my first posting in Delhi, providence had a plan to induct me in the Service by reporting to the same lady who was holding charge of Deputy Commissioner (P & V), New Delhi at that time. I recognized her and now knew her name to be Ms. Parveen Mahajan. Years in service made me understand that she was very dignified officer and accomplished in her own right. With passage of time and after I voluntarily left the service she went on to become the first Chairperson of the service. I got a chance to work with her at CEGAT, New Delhi.
Seizure of foreign currency hidden inside cricket bats at IGI Airport, Delhi in 1992.
Years went on in the Service, with ups and downs as usual in the life of any officer. Fortunately, there were more ups than downs.
1986 batch of the Indian Revenue Service at a get-together.
However, the premature end of service came at least twelve years before the due date of superannuation for me in the year 2008.
I had taken over the charge of Additional Director General (ADG), DGCEI, Bangalore, in the year 2006 when it was the smallest Zonal Unit and under-performing to boot. Its detection and recoveries were even lesser than the Regional Unit of Pune.
By the year 2008, I had been away from my family for almost 6 years. In two years at Bangalore, I had with my team made the Unit as the highest performing through robust detection of Service Tax cases. This included considering taxing of proceeds from organizing Lottery which led to Martin Lottery case and has thereafter been always treated as having potential of reoccurring revenue of more than One Thousand Five Hundred Crores (1500 Crores) per year and has led to even change in the definition of 'actionable claim' under GST.
I ensured that most of the staff working in DGCEI, Bangalore got their Presidential award which was till then was a rare occurrence.
After being away from family for six years, I made requests for the first time in my career for transfer to Delhi or around and had even in hand certain acceptances for deputation positions also. But same were rejected on the ground of a Transfer Policy made out by the then Finance Minister Shri P. C. Chidambaram.
My children who were left behind in Delhi where my wife was working as a Teacher were in school when I left and had become college pass outs when I decided to quit. Never did I try to meet any one over my request which were initially due to my mother suffering from cancer and being treated in Delhi.
The expense of two establishments had left the family with little savings. At that time I was reminded of the words of the first Chairman of the Service whom I had occasion to meet in NACEN. It was time to quit at the zenith of my career rather than begging for posting.
March 28, 2008, my last working day with the Indian Revenue Service at DGCEI, Bangalore.
But all decisions in life I had taken with divine approval and mandate. This time it was Sai Baba in Putta Purti (then in his worldly form) who was chosen as a medium to have my decision.
Once I had that approval, I worked out one or two options in the Corporate world through interviews. Whatever was learnt through Department was always coming handy.
I finally decided to put in my papers to join Reliance Haryana SEZ in Delhi, to be with my family. The concern was a semi-government company and though the project got called off, it provided me sufficient financial cushion to launch my further career in 'legal and consultancy profession' for which nature had prepared me years back when I was a JDR in CEGAT, New Delhi.
Life has always taken interesting turns and had so much to offer. The Department has sweet memories to carry forward and it was always a great learning experience gained by rotating myself from position to position and from Ministry to Ministry.
March 28, 2008, at DGCEI, Bangalore: leaving the office for the last time.
I have enjoyed the freedom afforded to me after voluntary retirement to the hilt by having multiple roles of being a journalist, columnist, author of legal texts, advocate and astrologer and talk shows on T.V. and other media and at varied seminars.
Astrology, which I picked up as a hobby while in service in 1994, enabled me to earn an all India silver medal and to represent India in Geneva Festival and at the United Nations. It has helped me in making friends internationally.
Awarded a silver medal in astrology by Finance Minister, Shri P. Chidambaram.
I am indebted to the service for having shown me charges from Kashmir to Kanyakumari and for having allowed to study abroad and visit more than 30 countries for investigation or for holding textile exhibitions.
My advice to all the officers in service will be to use the great opportunity they get of being part of this many faceted service which has presence in coasts, borders, hinterlands, seas, air and even overseas and to create their own intellectual worth. Do have your own hobbies and stay away from politics of any kind. With that success will touch their feet anywhere. Live with dignity and treat others the same way.
Enjoyed write-up cum journey
Permalink Submitted by Jaipal SIngh Rathore on October 3, 2018 - 12:58
Enjoyed write-up cum journey of life..it's quite inspiring. It's not an easy decision to leave golden career when you're at peak but there is a saying that you can't fly and roar in the sky unless don't leave the branch. Same is with me, started career with Indian railways as section officer and then joined department and served for 21 years but not satisfied the way life was going(getting retire at the age of 60 & doing mundane work) so took VRS at RIL SEZ Jamnagar when still 14 years service was to go. My friend who launched a company few year back at that time was doing pretty well, offered me a coveted position as Head Corporate Affairs, accepted and pursuing for last five years along with hobbies of trekking/hiking/marathon/writing/social service and making new friends everyday.
The biggest risk is not taking any risk.
A very inspirational read sir
Permalink Submitted by Nikita Jaju on October 3, 2018 - 17:24
A very inspirational read sir. Hats off to officers like you who give all of their self to the nation unconditionally.


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The Hill: "The Year of Witness" .. These are the strangest political stories in America during 2021 | Politics news
Puerto Rico and its plan to get out of bankruptcy, but without harming pensions | Video
Jennifer Coolidge Describes How Ariana Grande, Jimmy Fallon Helped Her Career Lull
The American newspaper, The Hill, said that in the midst of the epidemic, growing concerns about the future of democracy, an erratic economic recovery, and a former president refusing to step down, politics in the United States during the year 2021 was full of anxiety and anger.
But beyond the bleak headlines, some stories remind us that politics in this country can be an exercise full of wit, as it can attract the incompetent, the weird, the fraudulent and the totally uncommon to run for political office, serve in public office, or try to influence the national debate.
Here are the strangest stories that have marked the American political scene over the past year:
Utah governor threatens impeachment because of his name
Last August, an angry but unidentified citizen sent a letter to Republican Governor Spencer Cox asking him to change his last name or else "all decent people" in his home state would campaign to remove him from office.
"I don't know if you know this but when people say your nickname it sounds like another obscene word!" Cox wrote in his online message. "Because of your reluctance to change your hateful, filthy, obscene nickname, I and thousands of other Utahns will protest and will not We'll stop until you change your gruesome nickname to something a little less obscene."
A prominent Democrat loses to a truck driver
New Jersey Senate Speaker Stephen Sweeney, a Democrat, was for years considered one of the state's most powerful political figures, a nuisance to conservatives – Democrats and Republicans alike – with whom he clashed over just about everything.
But even so, this seasoned politician couldn't stand a commercial truck driver named Ed Doer, who spent little money on his Republican presidential campaign and so much effort knocking on voters' doors, denying his rival the chance to be re-elected in November. the past.
Fake Florida Filter
Republican Alan Garcia managed to win a Florida county during the 2020 election by a slim margin of 32 votes over Democratic incumbent Jose Javier Rodriguez, but a third candidate, Alex Rodriguez, received 6,382 votes.
It later turned out that Rodriguez had been recruited and pushed by former Republican Senator Frank Artells to run for the seat, and his mission was not to win, but merely to confuse voters and make them think they were voting for Democratic incumbent Jose Javier Rodriguez.
The authorities have brought 3 criminal charges against Artels, including transferring more than 44,000 dollars to the fake candidate, including paying the latter 10,000 dollars for a used Range Rover that did not even exist.
"Key" to the success of Michigan State Representative
Michigan Democratic Representative Jewell Jones faced a major problem last September when he was arrested for violating the terms of his pledge not to drive under the influence, but he made matters worse after he appeared in prison with a wrench in one of his feet.
This move led to two new charges against Jones, one of bringing a weapon to prison and one linked to his attempted escape, and the two new charges could send this power worker away into prison for half a decade.
The eternally young candidate
Audrey Clement has run multiple times in Arlington, Virginia, but her potential voters are a little confused about her age. This "permanent nominee" has told the media in the past that she's in her mid-50s, but when a reporter from The Washington Post looked through the records It became clear to her that she was born in 1949, 20 years before the date she claimed, and when asked about this contradiction, she relied on the Fifth Amendment of the American Constitution, which provides for the right of individuals not to incriminate themselves.
Father's heartbreaking tribute to five-year-old son
Golden State halts the Jazz streak
A draft executive order never signed by former President Donald Trump from December 2020 would have ordered the Defense Department to seize voting machines 'effective immediately' and appoint...
Posted at 17:55 ET (22:55 GMT) Friday, January 21, 2022 playing 2:56 Posted at 17:40 ET (22:40 GMT) Friday, January 21, 2022 2:49 Posted at 17:37 ET (22:37...
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Wealthy home workers retreat to their sheds
Robert Tonyan's three touchdowns lift Green Bay Packers to win over Atlanta Falcons and 4-0 start


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text:
Editorial Decision
Open Access Book
Unethical Behaviour
International Journal of Translational Science
Vol: 2016 Issue: 1
Published In: January 2016
Immunoassays for Affinity, Avidity and Competition: Methods to Evaluate Monoclonal (and Polyclonal) Antibodies Useful Assays in Development of New Antibodies, Better Understanding of Commercial Antibodies: Their Usefulness in Specific Aims of Research [SOP]
Article No: 1 Page: 131-134 doi: https://doi.org/10.13052/ijts2246-8765.2016.001
Read other article:
Immunoassays for Affinity, Avidity and Competition: Methods to Evaluate Monoclonal (and Polyclonal) Antibodies Useful Assays in Development of New Antibodies, Better Understanding of Commercial Antibodies: Their Usefulness in Specific Aims of Research
Received 20 October 2015; Accepted 25 October 2015; Publication 3 February 2016
Anita Lewis Antes
New Jersey Medical School/Rutgers University/Newark NJ E-mail: antesan@njms.rutgers.edu
ELISA (enzyme linked immune absorbant assay)
Enzyme-linked immunosorbent assay (ELISA) remains the most common method to identify clones of cells during the development of monoclonal antibodies. This technique is convenient for the rapid screening of large numbers of clones, subsequent to the fusion of splenocytes of immunized mice or rabbits with immortal myeloma cells. In general, when screening for the production of the desired antibody, an antibody to the target protein or an antibody from another host is generally unavailable. Thus, the standard test measures reactivity of attachment passively to the target antigen, and reporting of that attachment using a secondary antibody reporter (sandwich assay). Slightly better is the ELISA, which reacts the monoclonal within the cell culture (supernatant) with the antigen and then applies the mix to plates coated with an antibody against the target, but from another host species. In this way, the ability of the test clones to capture the target may be examined. This method is required when preparing antibodies for immunoprecipitation.
The sandwich assay may leave the investigator with as many as 100 or more prospective clones to further characterize. To identify clones for further investigation of the target antigen, cell supernatants may be too dilute. Thus, the expansion of this enormous number of clones for subsequent purification of the antibodies would be a daunting task. Here we offer EIA-based assays which simplify the process of excluding the majority of clones which will not serve the researcher's requirements. All the assays can be done from a single stock of each clone of as little as 2 ml volume.
1.1 Hybridoma Cells Propagation of Supernatants
Single cell subclones of candidate hybridomas are seeded at 1 × 105 cells/ 60 mm dish (or 2 × 106 cells/100 mm dish). Cell supernatants are collected 4–5 days post-seeding and then clarified by centrifugation at 5000 rpm for 5 minutes at 4°C. This is followed by filtering through a 0.2 micron sterile cellulose acetate membrane syringe filter. Supernatants should be stored at 4°C.
1.2 ELISA based Characterization of Monoclonal Antibody Epitopes
1.2.1 Affinity assay
Greiner flat bottom, non-tissue culture 96-well plates are coated overnight with 50–100 ng/well of the antigen. The plates are incubated overnight at 4°C. Row A will not be coated and will represent the evaluation of non-specific binding.
Plate surfaces are blocked with 100 μl/well PBS/0.1% Tween 20/1% BSA (Sigma), 1 hour at room temperature.
After thorough wash with PBS/Tween 20, 50 μl diluent (PBS) is added to wells C1 through H12; 100 μl of filtered cell supernatant is added to row B wells in triplicates. Using a 12 channel pipettor, 50 μl from row B is added to row C, mixed, and the process continued through row H, where the last 50 μl is discarded after diluting with the samples from row G. Note: 50 μl of cell supernatant, neat (undiluted), is also added to row A.
Incubation is performed at RT for 2 hours, followed by thorough wash with PBS/Tween. Secondary antibody Goat anti-Mouse H+L IgG conjugated to alkaline phosphatase (Jackson Immunoresearch) is applied at <0.5 μg/well, incubated at room temperature for 40 minutes. After thorough washing, the substrate is added to each well and the plates are read for alkaline phosphatase on the plate reader using a 405 nm filter.
1.2.2 Avidity assay
Sample plates are prepared as follows: 50 μl of diluent is added to all wells of a blank plate, except row A, into which 100 μl of target antigen (0.05–0.1 ng/μl) is placed.
Using a 12 channel pipettor, 50 μl from row A is mixed with the diluent in row B. The process is repeated up to row H, where the final 50 μl is removed and discarded. As previously described, plates are incubated overnight at 4°C, then blocked, and washed. Cell supernatants, in triplicate, undiluted, are added, 100 μl/well, and then incubated as described in the affinity assay. Average absorbance values are plotted vs antigen dilution value, and cell supernantants displaying linear response to the lowest antigen value are deemed most avid.
1.2.3 Competition assay
Results from theAffinityAssay are used to determine the concentration of each cell supernatants to be used in the experiment. Plotting the absorbance values vs the dilution value of the supernantants, the 50% point will be determined for each sample. Each of the cell supernatants will be diluted to reflect the concentration of 50% absorbance value. The assay will be performed in triplicate. First, a replicate plate with the diluted supernatants is prepared by dispensing 50 μl each of two supernatants (a pair) into the wells of a blank plate. The samples are then transferred to a standard coated plate, and the ELISA proceeded as described previously. Absorbance values of paired supernatants equal or greater than their 50% value alone suggested antibodies which could be used in capture reporter assays.
[1] Lequin, R. M. (2005). "Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)". Clinical Chemistry 51 (12): 2415–8.
[2] Engvall, Eva; Perlmann, Peter (1971). "Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G". Immunochemistry 8 (9): 871–4.
[3] Van Weemen, B.K.; Schuurs, A.H.W.M. (1971). "Immunoassay using antigen—enzyme conjugates". FEBS Letters 15 (3): 232–236.
[4] Kragstrup, Tue W; Vorup-Jensen, Thomas; Deleuran, Bent; Hvid, Malene (2013). "A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients". Springer-Plus 2 (1): 263.
A. L. Antes Research Teaching Specialist II, Research Teaching Specialist III, UMDNJ, Newark, New Jersey, USA.
Inventions: Property of the New York State Research Foundation Licensed non-exclusively to Oncogene Science.
R-264: "Monoclonal Antibodies to the Rouse Sarcoma Virus Transforming Protein", R-567: "Hybridoma AZA2E8" Epstein-Barr Nuclear Antigen Monoclonal Antibody.
International Journal of Translational Science, Vol. 1, 131–134.
doi: 10.13052/ijts2246-8765.2016.001
© 2016 River Publishers. All rights reserved.
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text:
Shelf Seas Modelling Programme
The European Regional Seas Ecosystem Model
The SSB website will be retired at the end of 2023, up to date information can be found at https://www.pml.ac.uk/science/Projects/ERSEM-(European-Regional-Seas-Ecosystem-Model)
The European Regional Seas Ecosystem Model (ERSEM)
ERSEM was initially developed in two projects, funded by the EU Marine Science and Technology programme: ERSEM (1990-1993), and ERSEM II (1993-1996), both under the leadership of Job Baretta at NIOZ (NL). In addition to NIOZ, these initial projects involved Plymouth Marine Laboratory (UK), the Universities of Oldenburg (DE), Hamburg (DE), Strathclyde (UK) and Aberdeen (UK), Marine Laboratory Aberdeen, Ecological Modelling Centre (DK) and CEAB (ES).
The earlier ambitions of the programme were significant, not only in coupling benthic and pelagic processes in a physical context and developing a prognostic model, but also in extending up the food chain to age-structured mesozooplankton models, pelagic and demersal fish, and even seabirds.
Each of the initial ERSEM projects produced a special issue describing model systems and results. After cessation of the ERSEM projects model development occurred more independently in three main centres: NIOZ and PML, producing variants using the ERSEM name, and at Bologna, where ERSEM formed the basis of the Biogeochemical Flux Model (BFM). Since 1995, we estimate that in excess of 200 peer reviewed papers have been published using ERSEM. The ERSEM model now aspires to reach beyond its name, addressing biogeochemical and ecological systems in many applications in global regional seas, and more recently the global ocean, engaging in a range of heuristic, predictive and impact studies.
The SSB-ERSEM package includes a re-locatable biogeochemical water column model based on GOTM-ERSEM. The code and model setups presented have been developed as a community modelling tool for the NERC Defra UK Shelf Sea Biogeochemistry program. The first release of the model provides a baseline model version upon which we can build and develop new process model descriptions, drawing on the experimental and observational activities undertaken by the wider community. We see developing a better synergy between modelling and experimental studies as key to progress over the next decade, hence this system can by run on a PC and provides an entry level modelling tool for non-specialist modellers to engage in process modelling.
Register and download code
Shelf Sea Biogeochemistry programme website - BODC
Shelf seas modelling progamme introduction
NERC Shelf Sea Biogeochemistry programme website
General Ocean Turbulence Model (GOTM)


text:
Карлос А. Кариљо има више значења:

 Општина Карлос А. Кариљо (Веракруз), општина у савезној држави Веракруз у Мексику
 Карлос А. Кариљо (Карлос А. Кариљо, Веракруз), насеље у савезној држави Веракруз у Мексику

redpajama_set_name: RedPajamaWikipedia


Will You Be Rich One Day? What are the signs of success to look for?
Well, it depends on your personal qualities. If you are on the right track, then success will surely come your way.
In this article, you will learn 3 characteristics that are shared by all successful people. If you have similar characteristics, then you have a strong potential to be one of them.
SIGN#1: You Have Written Goals.
Goal setting seems so intuitive and therefore unnecessary to even talk about it.
84% of the people had no specific goals at all!
14% had goals but they were not in written form.
Only 3% has clearly written goals and had plans to accomplish them.
As you can see, the vast majority of the people don't have clearly written goals. This is a very embarrassing statistical figure because numerous researchers have found that if you write down your goals, you are much more likely to achieve them.
For instance, Dr. Gail Matthews, a psychology professor at the Dominican University in California found out that you are 42% more likely to achieve your goals and dreams, simply by writing them down on a regular basis!
After all, you can't hit the target that you don't see!
While this could be partially true, in reality, when you don't write down your goals, they are often vague.
However, these goals are simply too vague and they don't urge you to take specific actions today.
Also, if you decide to keep your goals in your mind, they are likely to get jumbled with the 100s of other thoughts that you have every second.
In fact, 25% of the New Year resolutions are dropped within the 1st week of the year.
So, as you can see, if you don't write down your goals and you aren't serious about achieving them, the chances of achieving the same are very little.
Coming back to the Harvard study (the one we had discussed earlier), 3% of the people who had written down their goals were earning 10 times more than the rest 97%!
Therefore, if you have clearly written goals, you are ahead of 97% of the population and on the right path to success.
Nowadays, positive psychology is very popular.
Quotes like these can be very deceptive. Earlier, I used to fall into the trap of false optimism as well.
The above statements are usually far from what we really can achieve.
It is good to be optimistic.
At the same time, it is important for you to do your homework - study the market, study the situation, study the facts and be aware of the reality.
"Everything you want is coming. Relax and let the universe pick the timing and the way. You just need to trust that what you want is coming, and watch how fast it comes." Abraham Hicks.
Such quotes can cause you to live in a delusion.
As you can see, Branson doesn't give you a false sense of optimism. Not everything will be positive. There will definitely be ups and downs. But it is still possible.
So what does it mean to be a realistic optimist?
You've done your homework (research). You are aware of the facts and reality.
You see that there are pros and cons of your idea.
In such a situation of uncertainty you say "Yes".
If you follow the above steps, then you are taking calculated risks. You are better prepared to face the hardships and persist till you achieve your rewards and goals in the end.
If you are a Realistic Optimist and you take calculated risks, it is a clear sign that you are on the right path to success.
SIGN#3: You Turn Your Ideas Into Actions QUICKLY!
Most people have good ideas and plans for achieving them, SOMEDAY. However, most people don't take determined actions to turn these ideas into action.
Bill Gates wrote a book "Business @ the Speed of Thought." Bill Gates and several other billionaires often stress the importance of turning ideas into actions QUICKLY!
What Woody Allen highlights here is that most people don't even take the first step to turn their dreams into reality.
Therefore, if you are the type of person who puts plans into actions quickly, you are ahead of the majority of the people.


PwC's cyber security practice has worked closely with BAE Systems and other members of the security community, along with the UK's National Cyber Security Centre (NCSC), to uncover and disrupt what is thought to be one of the largest ever sustained global cyber espionage campaigns in an operation referred to as 'Operation Cloud Hopper'.
Since late 2016, PwC and BAE Systems have been collaborating to research the threat, brief the global security community and assist known victims. The threat actor behind the campaign is widely known within the security community as 'APT10', referred to within PwC UK as 'Red Apollo'.
The espionage campaign has targeted managed IT service providers (MSPs), allowing the APT10 group unprecedented potential access to the intellectual property and sensitive data of those MSPs and their clients globally. This indirect approach of reaching many through only a few targets demonstrates a new level of maturity in cyber espionage – so it's more important than ever to have a comprehensive view of all the threats your organisation might be exposed to, either directly or through your supply chain.
The sheer scale of the operation was uncovered through collaboration amongst organisations in the public and private sectors, but is still only likely to reflect a small portion of APT10's global operations. A number of Japanese organisations have also been targeted in a separate, simultaneous campaign by the same group, with APT10 masquerading as legitimate Japanese government entities to gain access.
More detail on the operation is included in our joint report with BAE Systems, available to download below. You can also download separate documents outlining the key indicators of compromise to check for and technical details relating to APT10.
For any questions on the operation or APT10 please contact our Threat Intelligence team, or for advice on protecting your organisation please contact Threat Detection and Response on the details below.


The Pfhorums • View topic - Weland stops working.
Board index ‹ Content Creation ‹ Mapping ‹ Weland stops working.
Weland used to work now it doesn't.
It crashes after creating a few lines, as though it can't handle creating a polygon even before I fill it.
We're talking a minute of use tops. Won't crash if it just sits there.
I get a message that states: "Weland has stopped working."
I have the same issue, but only on the Windows version (Windows 10 for me). The Mac version runs fine without a hitch, but I would like the option to use both.
Shocktart wrote: Running Windows 7.
Same as well, Windows 10 here. I use to be able to fix it by uninstalling GTK and reinstalling GKT (Don't know why this helped, just did) but now it no longer works for me.


text:
Q: Printing the function that prints True, but also prints false My problem with this code is that I want it to print Current Offence: Player, but also Current Defender: Cpu
Here is the code:
import random

Player = False
Cpu = False
user = input("Please enter [H]eads or [T]ails: ")

def flip_coin(Player, Cpu, user):
    # Flips coin to determine who has the ball first.
    COIN = ["H", "T"]
    coin = random.choice(COIN)
    print("Coin is on: " + str(coin))

    if coin == user:
        Player = True
        return "Player"
    else:
        Cpu = True
        return "Cpu"

print("Current Offence: " + str(flip_coin(Player, Cpu, user)))
print("Current Deffence: " + str(flip_coin(Player, Cpu, user)))

Output:
Please enter [H]eads or [T]ails: H
Coin is on: H
Current Offence: Player
Coin is on: H
Current Deffence: Player

Expected Output:
Please enter [H]eads or [T]ails: H
Coin is on: H
Current Offence: Player
Current Deffence: Cpu

How can I make it to where Current Offence is on Player, but also Current Deffence is on Cpu without it being on Player?

A: You only need one bool (or int) to hold all the information:
import random

players = ["Cpu", "Player"]

def flip_coin(coin):
    return random.choice("HT") == coin

user = input("Please enter [H]eads or [T]ails: ")
player = flip_coin(user)
print(f"Current Offence: {players[player]}")     # Note: True == 1
print(f"Current Defence: {players[not player]}") # and: False == 0


Work for Palo Alto Networks URL Filtering PAN-DB?
Learning about Palo Alto Networks URL Filtering PAN-DBf?
The visibility into what is actually happening on the network with respect to URL traffic.
I really don't have anything bad to say about the product, I highly recommend the product.
The firewall and the tools that are being developed for this platform are phenomenal! They are now coming out with a new compliment product to the PA - Magnify .. .check out this vendor... they will not let you down.
I am using the information to better tighten down security. I am also using the information to better form governance for this organization.
What I like about using Palo Alto for URL filtering is the fact that when you submit a change for a website to be placed in a different category, they are very quick to respond whether they believe your recommendation should be validated or denied.
What I dislike about Palo Alto for URL filtering is that when you do submit a chance for a different category, and it is denied, there is no reason why. Also, that it seems they dont look at the website in question and its obvious that the category should change, but it still gets denied the change.
Verify the websites being submitted for change, that the change is valid and accurate to its request.
We are solving issues with websites that are safe for school use being blocked by PA because the website is listed as not having a category, but when we request a change of category to usually education, it gets changed fairly quickly then our district can access those websites.
"Palo Alto Filtering is Excellent"
"URL Filtering at its finest"
Palo Alto enables network engineers to block malicous URL's and a variety of other categories such as C&C, parked, malware, websites. This is a great tool when utilized properly.
There should be a way to explode URL traffic to see individual links and websites an employee is going to, this helps in determining the activity that should be addressed.
This has millions and billions of malware sites that is already blocking and different categories to customize URL blocking or alerting. Great way to protect from all phishing and malware attacks.
None. Great product and awesome features PAN has.
Protecting our corporate and production environment with this great URL filtering. Before we had several user got affected to the phishing and malware attacks. After implementing this PAN URL filtering, none of the users got affected.
"Updates more frequently than others"
Frequent database updates make this product more desireable than the competitive products which update daily.
Suggested category changes submitted through their online form, as with competitor products still take too long to be approved.
Protecting staff from bad websites is a top priority.
Learning about Palo Alto Networks URL Filtering PAN-DB?
* We monitor all Palo Alto Networks URL Filtering PAN-DB reviews to prevent fraudulent reviews and keep review quality high. We do not post reviews by company employees or direct competitors. Validated reviews require the user to submit a screenshot of the product containing their user ID, in order to verify a user is an actual user of the product.
Hi there! Are you looking to implement a solution like Palo Alto Networks URL Filtering PAN-DBf?


Gordon Robertson was in a Hindu temple where he saw a woman worshiping and praying to a stone cow. As he was having negative thoughts about the idolatry, he heard a voice behind him say, "No one has ever told her." He turned around and was having an open-eye vision of Jesus.
He decided to become a missionary. He returned to the USA and continued with his successful legal career. Soon he lost interest in his missionary plans.
Gordon Robertson had never prayed for anyone in his congregation to be healed. Then, one day while he was preaching, the Lord told him that He wanted to heal the sick that day. A 12-year-old boy's mother picked her child up ; he had never walked a day in his life. Mr. Robertson commanded the polio symptoms to leave and they did. Watch the video below.
Watch the entire interview between Gordon Robertson and Sid Roth.


text:
Sniper Advanced Targeting Pod demonstrates Maverick missile compatibility
Sniper Advanced Targeting Pod ...
October 10, 2006 The Sniper Advanced Targeting Pod (ATP) is the targeting system of choice for both the U.S. Air Force and Air National Guard and recently became an even more valuable bit of kit when it successfully demonstrated its compatibility with the launch of a Maverick missile from an adjacent A-10C wing pylon. Combat proven on the F-15E and F-16, Sniper's advanced targeting technology and features are changing the way the armed forces operate in theatre by providing new capabilities in non-traditional intelligence, surveillance, and reconnaissance (ISR). The Sniper is understandably very sensitive – in order to do its job, it contains a high-resolution, mid wave 3rd generation forward looking infrared (FLIR), a dual-mode laser and a CCD-TV along with a laser spot tracker and a laser marker. The advanced image processing algorithms, combined with rock steady stabilization techniques, provide cutting-edge performance but there are obvious issues in firing the rockets it does the precision strike mission targeting for when they are just a few inches away. The ability to fire missiles so close to the Sniper ATP uniquely qualifies Sniper for this weapon configuration, doubling the previous A-10C Maverick loadout capabilities.
Sniper provides vastly improved long-range target detection/identification and continuous stabilized surveillance in support of ground forces. For the first time, aircrews can detect and identify many classes of improvised explosive devices (IEDs), weapon caches, and individuals carrying armaments, all outside jet noise ranges. The superior imagery and JDAM-quality coordinates provided by Sniper keep aircrews out of the range of threat air defenses in their defining moments.
Fully compatible with the latest standoff weaponry, the pod provides automatic tracking and laser designation of tactical size targets via real-time imagery presented on cockpit displays. Likewise, the supersonic, low-observable design results in a substantial reduction in drag and weight.
"The addition of Sniper ATP greatly enhances the capabilities of the A-10," said Ken Fuhr, Sniper ATP program director at Lockheed Martin Missiles and Fire Control, prime contractor for the U.S. Air Force-led ATP program. "It provides the A-10 the ability to deliver both laser-guided and J-series weapons while enhancing existing close air support capabilities and providing greater capability to positively identify targets and growth capability for future weapons."
The test was conducted in August by the U.S. Air Force's 46th Test Wing, 40th Flight Test Squadron at Eglin Air Force Base (AFB) in Ft. Walton Beach, FL. The Sniper ATP was mounted on an A-10C Precision Engagement aircraft adjacent to the Maverick missile mounted on the LAU-88 missile rail. In this configuration, Sniper ATP is approximately 15 inches from the missile body. Test pilots from the 40th Test Squadron at Eglin AFB, FL, and the 422nd Test Squadron at Nellis AFB, NV, reported that they are impressed with Sniper ATP's performance during the developmental and operational flight tests of the A-10C. The A-10C's avionics upgrade and targeting pod integration are part of the Precision Engagement (PE) program, led by Lockheed Martin Systems Integration-Owego in New York.
Sniper ATPs are currently flying on the U.S. Air Force F-15E, F-16 and A-10 and are set to fly on the B-1 this month. Unlike other pods, Sniper ATP features a unique common aperture that eliminates boresight errors, ensures image stability even at supersonic speeds and has the most precise geo-location accuracy.
Additionally, Sniper ATP's true two-level maintenance eliminates expensive intermediate-level test equipment. Sniper ATPs have proven superior in tens of thousands of flight hours in thousands of sorties in support of Operations Iraqi Freedom and Enduring Freedom.
MilitarySniper
Loyal Wingman combat drone completes first high-speed taxi tests
Aerojet Rocketdyne hypersonic scramjet engine sets new thrust record
Artificial intelligence co-pilots military jet for the first time
LiquidPiston's "inside-out" rotary X-Engine wins Army research contract


text:
The Alliance Alive Releases In Japan On March 30, 2017
By Sato December 1, 2016
FuRyu announced during a Famitsu live stream that their upcoming Nintendo 3DS RPG, The Alliance Alive, will release in Japan on March 30, 2017. A demo event will also take place in Japan on December 23, 2016. [Thanks, Hachima.]
The above is a peek at some pre-order bonuses and store-exclusive bonuses that will be available for the game.
The demonstration event will take place on December 23, 2016, featuring a talk session with the developers and other guests.
Famitsu will also host their next live stream for the game on December 8 at 9:30pm JST, which will be the first gameplay showing for the game.
The Alliance Alive releases in Japan on March 30, 2017 for Nintendo 3DS.
More in Nintendo 3DS
This Week in Sales: Nintendo Switch and Pokemon Sword & Shield Off to a Strong Start in 2020
By Sato 6 days ago 0
Dragon Quest XI S' Most Popular Character Overseas Is Sylvando
By Alistair Wong January 10, 2020 0


text:
Two South Shore women answer the call for tasty, gluten-free baked goods
Valerie A. Russo
The number of people who have food allergies is growing, creating a demand for tasty food that is safe for people with food allergies and digestive ailments. Two local business owners – Carolyn Shannon of Cal's Baking Company in Hingham and Heather Diamond of Diamond Baking Co. in Weymouth - have created baking mixes and custom cakes for this growing market.
An estimated 12 million people in the United States have food allergies, according to a 2008 report from the Centers for Disease Control and Prevention. The figure includes 3 million children – four out of 100, an 18 percent increase from a decade ago. An additional 3 million Americans have celiac disease, the inability to absorb nutrients if they eat any foods containing gluten.
Whether these statistics indicate an overall increase in sensitivities to certain foods or an increase in testing and better reporting of food allergies, the result is the same: greater demand for tasty food that is safe for people with food allergies and digestive ailments.
Two local business owners – Carolyn Shannon of Cal's Baking Company in Hingham and Heather Diamond of Diamond Baking Co. in Weymouth – have stepped up to the plate with original baking mixes and custom cakes for this growing market.
One smart cookie
For 8-year-old Kevin Shannon of Hingham, the cure for chronic eczema wasn't a salve or steroid cream prescribed by an allergist. It was a strict diet of gluten- and casein-free foods, including chocolate chip cookies made by his mother from her gluten-free recipe.
The cookies were the first of many gluten-free baked goods that Carolyn Shannon made for Kevin and her husband, Dan, who were tested for food allergies and found to be sensitive to the gluten protein in wheat and the casein protein in milk and butter.
"Within two weeks of being on a gluten-free diet, Kevin's eczema and Dan's joint pain and stomach aches were completely gone. We were true believers," said Shannon.
Since the diagnosis two years ago, Shannon has developed new recipes and adapted some of her mother's recipes to make gluten- and casein-free cakes, cookies, brownies, pancakes, waffles, breads and pizza dough for her family and baking mixes for sale to the public.
Each recipe – which requires a slightly different proportion of brown rice flour, white rice flour and tapioca flour – took months to perfect.
"When I decided to make gluten-free bread, I thought, 'how hard can that be?'" said Shannon, an experienced baker.
"I put together a mix that I thought would work and put it in the oven. I swear it weighed 10 pounds, and it was horrible tasting. It took me months and months to develop the bread mix and pizza dough mix, the newest additions to my product line."
But the time and effort was worthwhile, Shannon said, because her products tasted so much better than the gluten-free mixes and frozen baked goods available in stores.
"When you start a gluten-free diet, you buy everything gluten free that you can find. What you quickly find is that the products out there are horrible. You think, 'this can't be how I have to eat for the rest of my life,'" Shannon said.
Shannon and her sons Brian, 6, and Nicholas, 9, are not sensitive to gluten or casein, but at home, the whole family eats gluten- and casein-free meals to make it easier for Kevin and Dan.
Shannon, who teaches first grade in the Whitman-Hanson Regional School District, initially had no plans to market her gluten-free baking mixes. Her husband was so impressed with the taste of her desserts, though, he persuaded her to sell the mixes.
They turned their garage into a commercial kitchen where Shannon prepares and packages the mixes she sells in local stores and through her Web site, www.calsbakingcompany.com. Although no one in her family is allergic to nuts, Shannon decided to make her mixes nut free (as well as gluten free and casein free) to appeal to more customers.
To meet the demand for her mixes, Shannon spends 24 to 32 hours a week on a variety of tasks: ordering supplies, printing and attaching labels to the empty bags, combining ingredients in a bowl with a big whisk and slotted spoon to make the mixes, filling and weighing the bags and testing each batch.
"Let's say I spend the day making 40 bags of chocolate cake mix. I would take one of those bags and bake a cake to make sure it comes out the way it is supposed to," she explained.
Customers need only a few additional ingredients, such as eggs, oil, milk and butter, to use the mixes. For casein-free milk, Shannon recommends Silk Soy Milk, a liquid, and Dari-free, a soy-free powder that can be used by people who are sensitive to soy products. For casein-free butter, she recommends Organic Smart Balance, and for chocolate chip cookies, she uses a soy-based brand of chips called Enjoy Life.
Cal's Baking Company mixes are available at Good Health Natural Foods in Quincy and Hanover, Healthy Appetites in Plymouth, All the Best Natural Foods in Cohasset and the Fruit Center Marketplace in Hingham and Milton.
Have it your way
On any given day, Heather Diamond of Milton is up to her elbows in gluten-free flour in her commercial kitchen one floor below Shaw's Supermarket in Weymouth.
"I've always loved baking; it's my passion. I've done it as far back as I can remember," said Diamond, who attended Johnson & Wales University for baking and pastry arts and worked as a pastry chef for five years before launching her own business, Diamond Baking Co., in 2008.
But you won't find her cakes, cookies, brownies, scones and tea breads on the shelves of supermarkets or other retail stores.
Diamond's baked goods are made to order, usually for special occasions, such as weddings, anniversaries, showers, birthdays, christenings, First Holy Communions, bar and bat mitzvahs and graduations.
"Gluten-free is growing in popularity," Diamond said. "There are more and more cases of celiac disease being diagnosed, but it's not just celiacs who are on a gluten-free diet. It supposedly helps kids who are autistic and anyone with Crohn's disease. Some people switch to a gluten-free diet because it makes them feel better, more energized."
Diamond also supplies gluten-free desserts to one local restaurant, Peppercornz On Main in Weymouth, and sells her gluten-free cookies, brownies and cupcakes from 2:30 to 6:30 p.m. Thursdays through October at the farmers'market in Plymouth.
Diamond's first experience with gluten-free baking was making a birthday cake for a friend with multiple allergies. In place of wheat flour, she substituted a mixture of three gluten-free products – brown rice flour, tapioca flour and potato starch. Instead of butter and cow's milk, she used canola oil and rice milk.
"It was fun and a challenge creating recipes that actually tasted good and were not too crumbly," Diamond said. "The wheat is going to bond it, and that's missing."
She also uses fresh local ingredients, such as organic cage-free eggs, stone ground organic chocolate and fruit from local farms.
To date, Diamond has developed a dozen recipes for cakes and more than a dozen recipes for cookies, brownies, muffins, scones and tea breads.
Don't ask for copies – her secret recipes are the result of many months of work and taste testing by friends and family to get the right proportions of the gluten-free ingredients.
"Every recipe is gluten free and nut free, but I can also make something dairy free, soy free or egg free for someone with multiple allergies," she said.
Her favorite recipe is for gluten-free, dairy-free carrot cake.
"When I give that cake to people who are not on a gluten-free diet, they say it's the best carrot cake they've ever tasted – melt-in-your-mouth moist. It has real carrots," she said.
Her baking schedule varies, depending upon orders. April through October are her busiest months for wedding cakes and wedding cupcake towers.
She makes just one wedding cake per day because she does everything herself, from the baking to the decorating to the delivery. Year-round, she makes gluten-free baked goods with 48 hours' notice. Customers can pick up orders at her commercial kitchen or receive delivery for orders more than $100.
Customers planning a wedding or other special event can schedule a tasting. For more information, see www.DiamondBakingCo.com.
The Patriot Ledger


Mini Mounting Plate with Single 15mm Rod Clamp 1906 is a simple plate with various functions, used with any industry standard 15mm rod. It includes two cold shoes on the plate, which allows to attach microphone, wireless receiver or articulating arm. Besides, it comes with an adjustable groove to keep the kit balance when using monitor or EVF. Body of the plate is equipped with several 1/4'' & 3/8'' mounting points for additional accessories attachment.


Join us for Swing Into Spring, our annual fundraiser celebration on May 10, 2019 to raise money to give women and girls a boost.
New mom? Expecting a baby? Join our FREE MomShare drop-in support group for women and infants, Tuesdays 1-2:30 p.m. or the 1st and 3rd Saturdays, 9:30 to 11:00 am.. Email Lisa at lfattori@med.umich.edu for topics and announcements.
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Consider job coaching whether you are looking for a job or seeking to upgrade.
There's room to talk at The Women's Center. Individuals with health insurance have the opportunity to decide between our sliding-fee counseling services or our new insurance-based counseling program.
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Don't worry. Just call us at 605.642.7973 and we will reprint your ticket and hold it for you at Will Call. We will also reprint your ticket at the box office the night of the event, but if possible, call us first (before 5 p.m.). Sometimes the box office lines get long and we would like to avoid delays. If you are in a group, it helps if we can get your seat number. If you don't have it, just let us know the name of the person who bought the tickets, and we'll handle the rest.
We process our new subscribers in the order in which they are received. New subscribers will be seated following our renewing subscribers.
Patrons who buy our Subscription Season tickets also receive adult ticket discounts on other non-subscription events automatically. Whether buying your non-season event ticket online or through our box office, the discount will be applied automatically.
All ticket sales are final and non-refundable. If you are unable to attend a show that you have a ticket for, feel free to give it to any of your friends or family. They are transferable. In the event that a performance is cancelled, all tickets will be refunded right away.
When purchasing tickets online, you will be able to select your seats through our interactive seating chart. Just below, is a view of our seating chart. Every ticket you purchase will give you the chance to select and reserve your seat(s).


text:
Q: Navigation Experimental is not unmounting components I am using navigation experimental for my react-native application's navigation. When I am pushing a repeating route in my stack I am checking it whether that already exists in my stack if it does I use replaceAt() of NavigationUtils to replace that page with the new route, but my component is not unmounting when I do this.
Expected Behaviour: When I replace a route that already exists in my stack then my navigation experimental should unmount the current route and should replace it with new route and by mounting that component again.
My Navigation Reducer is this.
 const initialNavState = {
       index: 0,
       routes: [{key:'Second',title:'Second'}],

     }

 export default function navigationState(state = initialNavState, action) {
      console.log('current routes:',state);

      switch (action.type) {

        case NAV_PUSH:

           console.log('Page requested to open :',action.state.key)

          // trying to push the route where we already are, no need to change a thing
           if (state.routes[state.index].key === (action.state.key && action.state.key)) return state;

         // ensure no duplicate keys

           const index=NavigationStateUtils.indexOf(state, action.state.key);
           console.log('DoesPage existed already in stack (index value if exists and -1 it doesnt):',index);

           if(index > -1)
            {
               console.log('got index in stack, goin to replace it',index)
               return  NavigationStateUtils.replaceAt(state,action.state.key,action.state);
            }

          return NavigationStateUtils.push(state, action.state);

       case NAV_POP:
                    if (state.index === 0 || state.routes.length === 1) return state
                       return NavigationStateUtils.pop(state)

       case NAV_JUMP_TO_KEY:
                       return NavigationStateUtils.jumpTo(state, action.key)

       case NAV_JUMP_TO_INDEX:
                       return NavigationStateUtils.jumpToIndex(state, action.index)

       case NAV_RESET:
                       return {
        ...state,
        index: action.index,
        routes: action.routes
    }

          default:return state
   }
  }

Please tell me where I am getting wrong in the implementation.


text:
Krenta (cyr. Крента) – wieś w Serbii, w okręgu zajeczarskim, w gminie Knjaževac. W 2011 roku liczyła 67 mieszkańców.

Przypisy

Miejscowości w okręgu zajeczarskim


text:
Manchester United: 2018 Tour
Date: 13th July 2018 at 5:39pm
Written by: Guest Blogger
As part of the 2018 tour, Old Trafford's Manchester United football club will play five games when they return in July to the United States. One of the five games would be against their arch rival, Liverpool.
Teams to be faced by Manchester United on Tour
The Reds are scheduled to fly to the United States on the 15th of July after which they will be playing two friendly matches against Club America on the 19th of July, and San Jose Earthquakes on the 22nd of July. After the friendlies they would be taking part in three International Champions Cup matches, playing against AC Milan and Liverpool on the 25th of July, and Real Madrid on the 28th of July.
Fixtures for the tour matches
1. Manchester United Vs Club America: July 19th @19:00 local time. Venue: Phoenix University Stadium. This will be the first game of the Tour 2018 presented by Aon. The Eagles, based in Mexico City, have previously played against Manchester United (the Reds) in the year 2015. During the face-off, which took place in Seattle, over 45,000 supporters were in attendance. Manchester United came out on top; with the match score being 1-0 after Morgan Scheiderlin scored the only goal in the match. The stadium capacity for this year's match would be the entire 75,000-capacity of the Phoenix University Stadium.
2. Manchester United Vs San Jose Earthquakes: July 22nd @14:00 local time. Venue: Levi's Stadium. This fixture has the potential for over 60,000 fans to be in attendance at the Levi's Stadium in Santa Clara, California. It would be the second match of the Tour 2018. Sanjose Earthquakes compete in the Western Conference of MLS. Based in San Jose, California, the San Jose Earthquakes have also previously tangoed with the Reds in 2015. The match took place at the California Memorial Stadium. Juan Mata, Memphis Depay, and Andreas Pereira scored the three goals of the match in favour of Manchester United 3-1 Sanjose Earthquakes.
3. Manchester United Vs AC Milan: July 25th @20:05 local time. Venue: Rosebowl Stadium. This will be the Red's third game of the Tour in the International Champions Cup. AC Milan came out on top back in 2004, in New Jersey, with a 9-8 win through a penalty shoot-out against Manchester United. That was the last time they played against the Reds in pre-season.
4. Manchester United Vs Liverpool: July 28th @17:05 local time. Venue: Michigan Stadium. This is the second biggest venue in the world. In the Premier League of last season, the Reds were undefeated against Jurgen Klopp's side. There was a goalless draw at Anfield after which a glorious victory of 2-1 happened at Old Trafford. This fixture of July 28 will be one of the fiercest rivalries in English football.
5. Manchester United Vs Real Madrid: July 31st @20:05 local time. Venue: Hard Rock Stadium. This will be the final match of the 2018 Tour. Manchester United will be playing against the winners of the Champions League (Real Madrid) in Miami. During the Champions cup which took place at Santa Clara, California, the Reds defeated Los Blancos on penalties. However, the Spanish side got their redemption in the UEFA Super Cup match the following month.
Viewing Channels for the fixtures
Do not miss a second of any of the matches as they would be streamed live globally on the internet at MUTV.com. The MUTV app is also available. In the UK and Ireland, Sky or Virgin Media's MUTV will also be airing the matches.
Where to purchase match tickets
You can get your tickets booked and take full advantage of a wide range of seating choices. You would be enabled to conveniently focus on your plans for a stateside trip to see the Reds live in action.


This handcrafted "Whirlygig" helicopter is made from Aromatic Cedar and Cypress hardwood. The top blade and tail rudder move and actually spins. Your little one will no doubt use their imagination with this toy. A great Waldorf Educational Toy! It is finished with a non-toxic high gloss finish, and as always can be personalized with your child's name, birth date or whatever would make it special to them. It measures 11" Long by 8" Wide by 4" High.


TATR is a 550+ mile, solo, self-supported bikepacking adventure through the Adirondack Mountains of New York State. The annual grand depart is the second Friday of September, or riders can tour the route at any time.
Last year's results are posted! A big congrats to Shane Kramer, current record holder. TATR is a difficult route, a true Adirondack adventure.
Follow TATR on Facebook or send an email to theadirondacktrailride@gmail.com to be added to our e-news list. Stay up to date on all things TATR!


Okay, Gateway wasn't going to be any help so I turned to the Interwebs. As you might recall from the start of this saga, I was not the only person who had the flickering monitor problem. Real Geeks had troubleshooted (troubleshot?) the problem to a specific chip on a specific board in the monitor. Apparently there is an overheating/poor-manufacturing problem that causes the chip to pull away from the board. But the chip could be reflowed. With the help of Lon Jungemann, I gave this a try. It did not work. So now, instead of a monitor that flickered, I had a monitor that just stayed black. Not necessarily an improvement.
Oh well, I tried. I was about to throw it in the trash recycle it responsibly when I recalled reading when I was searching for a fix that people did actually repair these. So on the off chance that someone would need parts, I put it on eBay. I didn't want to disappoint anyone, so it had a big, "not working. for parts only" in the listing. In fact, I didn't include any information on it at all. I figured that only someone who knew what they were doing would bid on so sparse a listing.
Maybe I could make $50 toward my next monitor.
That's right, $304.99 for my broken monitor. The winning bidder was John Harlan he goes by johnslcdrepair, johnhis11_computers, xhd3000repairman43560 as well as computerguru43560 (if you've found this post by searching for any of those names, you'll want to read the next parts of this post as well –how's that for foreshadowing?), and also had several of these monitors listed on eBay. So he knew what he was getting into. He asked some questions about how I had tried to reflow the chip before he bid, so everything seemed fine.
But he didn't pay right away, and after a week went by another bidder contacted me and said that he had recently been outbid on several of these monitors by John Harlan and wanted to know if I would sell the monitor to him if this sale fell through. We even agreed on a price (he started at $25 and I started at $304.99 and we met at $250). So I opened a "did not pay" dispute with eBay. Shortly thereafter John sent the money via PayPal. Now, in his defense, John did tell me that he was a little tight on money and would take a few days to pay (but not more than a week). But John did win the bid, and had sent payment, so we packaged the monitor up.
Yes, that's a screen covered with a towel then 1/2 inch thick Styrofoam then 1/8 inch thick plexiglass then 2 layers of bubble wrap then surrounded by air bags on all sides.
Read the continuing saga in part three.
Were you packing that monitor for the mail, or for the apocalypse?
Yeah -- two giant hands now, too.
It was an entire evening of packing, and the use of lots and lots and lots of tape.
It's not the hand that bothers me so much, it's that finger. Why'd I draw the knuckle lines and a fingernail?
It's a very unflattering cartoon.
I'm on the edge of my seat! I can't wait until tomorrow's installment of SOYJH!
This is like waiting for the season finale of Battlestar Galactica!
Are you sure this is done in five parts or ten?
Most search engines are smarter than that now. Repeated words probably lower your page rank for that term if they are out of context.
Nuts. Okay I'll stop. But you are on the first page on Google when you search for his name. I was just trying to get you higher on the page.
I thought you were doing the Bloody Mary thing and trying to make him appear in a mirror.


Experience the city's street food beyond hot dog carts and pretzel stands on this New York food tour.
Select from tours in Midtown or Downtown.
Your friendly, knowledgeable guides have been featured in the New York Times, USA Today online, WNBC and Time Out New York.
Enjoy 6 food tastings, which may include banh mi Vietnamese sandwiches, Korean short ribs, Mexican chocolate brownies, schnitzel, and more from a variety of the many delicious food carts in the area.
Learn about these businesses from their proprietors and uniquely explore the neighborhood.
Discover the culinary creations of some of New York's best curbside kitchens!
This tour will take you on a strolling lunch in Midtown or Lower Manhattan to sample an international buffet of offerings, observe the constructive genius that cram full kitchens into improbably small carts, and hear the stories of their proprietors - from struggling immigrants to culinary school drop-outs, to former 4-star restaurant chefs.
Along the way, learn about the history of the neighborhood and the challenges of running these thriving but feverishly demanding small businesses.
This tour offers a rotating menu from day to day to represent the wide variety of delicious options that are available from the food carts and trucks.
You will be accompanied by other tour participants, with a maximum of 16 per tour. For groups larger than 6 please contact Cloud 9 Living directly for pricing.
Tours run rain or shine. There will be no cancellations due to weather unless experience provider cancels the tour due to safety concerns.
Wear comfortable shoes and dress appropriately for the weather.
For this experience, it is recommended to request bookings at least 14 days in advance of intended date.
Once you have scheduled a date and received a booking confirmation, no refunds will be given but rescheduling your experience is allowed up to 72 hours prior to the Experience for a $10 per ticket fee payable to the experience provider. Failure to appear on your booked date, cancel, or reschedule at least 72 hours prior to your booked date will result in the loss of the value of your experience.
This tours rain or shine, so please plan ahead and dress for the weather. In extreme cases, however, the experience provider does reserve the right to cancel tours if the weather conditions are dangerous. You would be contacted at least 2.5 hours before your tour if this were the case.
This tour involves approximately one to two miles of walking.
Yes, there will be opportunities to use the restroom at least once during the tour.
Yes! There will be at least one opportunity to sit down during the tour.
Gratuity for your guide is not included but is always appreciated.
Our guide (Brian Hoffman) was very friendly and knowledgeable, and made the interesting and tasty 2 hours fly by. One of the highlights of our trip to New York.
I absolutely loved my walking tour of the street vendors in New York. I didn't expect to get so much food or gain so much knowledgce about how it all works. Very, very happy and would highly recommend to anyone.
We will never look at a food carts the same, after taking the Financial District Food Cart's Tour within New York City. Brian was our guide, and did an outstanding job educating us on the various aspects of New York City's tastiest food carts! As one who knows his subject, Brian was witty, knowledgeable and friendly. His enthusiasm was infectious as he led us through the streets of New York's financial district, lending us some very tasty food cart cuisine! When you think of it, those food carts located within the financial district would likely be the very best, because they, unlike those in touristy Manhattan, survive on repeat/local business (you know...those white collar types who really know what good food is). Thus, our tour was one we'll remember, not only because of the tasty treats we were given, but because of the wealth of interesting facts Brian provided about the cart's history, what one must go through to even get a cart on the street, the cleanliness factor of the carts, and even the history of many of the characters who operate them. Our tour was one of the most fun things we did while in The Big Apple (and we visit annually and do lots of stuff). The food from the several carts was tasty (we're not talking hot dogs here), and Brian was the perfect guide, gentleman and entertainer! Plan to walk a bit...not a tour for those who have difficulty navigating the sidewalk. We heartily recommend this tour without abandon!
What a serendipitous experience. The eight members of our group, our guide Brian and certainly the food made for a magical time in midtown Manhattan.
For the price of the tour, we felt very little was offered.
Well done you should feel proud.. Thanks for having us..
My boyfriend and I had a blast! Not only was our tour guide friendly and fun, but very informative. The food was amazing and we were able to contribute to the local businesses. Will be definitely be doing this again.


Join us one of our fabulous Loving Welsh Food tours. Enjoy Cardiff's delicious food and drink while visiting our beautiful capital city and discovering its people, culture and traditions.
These Tours run every Friday and every Saturday from Friday 1st February / Sat 2nd February to Friday 29th November /Sat 30th November.
A walking & tasting tour including 8 tasting stops showcasing Welsh and Cardiff's cosmopolitan food.
The Cardiff Lunch & Landmarks Tour runs on the first Saturday of every month. The tour includes tastings and a 2 course lunch in different venues in Cardiff & Cardiff Bay. Our restaurants are chosen not only for their fabulous food but also their heritage and interest. We travel from Cardiff city centre to Cardiff Bay by water bus to explore what Cardiff Bay has to offer foodies for dessert.
The first date for our Cardiff Lunch & Landmarks Tour is Saturday 2nd February 2019 and runs on the first Saturday of every month.
Loving Welsh Food also offer Welsh cooking workshops for groups, where you get to make, bake and take home your very own Welshcakes. We cook in a fabulous venue overlooking the Principality Stadium right in the heart of Cardiff.
Group tours are available in the Garden of Cardiff – the Vale of Glamorgan including our Welsh Food & Drink Safari tour where you'll spend the day sampling some of the fabulous food and drink on offer and get to hear the growers and farmers' unique stories.
"Delicious tastings, lunch in beautiful surroundings and an entertaining tractor tour, ending with a welcome cup of tea and delicious cake in a beautiful farmhouse garden".
The Castles & Cuisine tour enables you to visit the beautiful and historic "Castell Coch" (Red Castle), enjoy a Welsh lunch in a traditional pub and then try our fabulous Welsh wine at a local vineyard.


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Monmouth County: The Monmouth County Sheriff's Office has been, and will remain, on heightened alert in preparation for the 14th anniversary of the September 11th attacks on Friday.
The Monmouth County Sheriff's Office routinely conducts security checks at critical infrastructures throughout Monmouth County. During the 14th anniversary of 9/11, law enforcement will have a greater presence and heightened vigilance. The sheriff's office will be supplementing local police patrols at various locations, including ferry terminals and other major transportation hubs. In addition, the sheriff's office K-9 teams will be sweeping ferry terminals and train stations, as well as major 9/11 remembrance observances, as requested. Sheriff's Marine #1 will also be out patrolling the waterways and conducting homeland security checks throughout the day.
As always, the Monmouth County Sheriff's Office asks that people remain vigilant and report any suspicious activities to the local authorities.


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How to buy Indian Instagram Followers ?
So, you need to simply visit www.Indianlikes.com and then click on to Indian Instagram followers, once you are done with the payment,after few hours you will see followers interacting with your profile, these followers are true followers as well as active users too.
How Indian likes focus on Indian followers only ?
Indian Instagram followers is their personal service, they usually have a good traffic ontheir website. They can easily promote your page to vast audience and can converts the users into your followers. These are real followers and you can easily target your audience as per the requirement.
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Yes these users are 100% active as these followers will get indulge with your page and will be interested in liking and commenting on your post. Indian likes is a trusted website and promise everyone to give trusted users or followers so there is no risk behind it. You can easily use that.


Lily Simpson runs The Detox Kitchen, whose boutique delis and specially designed health menus have won thousands of customers – including international celebrities such as Elle Macpherson, Sophie Dahl and Suki Waterhouse. She and consultant nutritionist Rob Hobson are devoted to their philosophy of great health through great food. Inside this book are 200 of Lily's exquisite recipes brightly delicious and packed full of flavour thanks to her clever combinations of herbs, spices and oils. All wheat‐, dairy‐ and refined sugar‐free, you'll find recipes for invigorating breakfasts, zingy raw salads, delicious snacks and dips, vibrant fish and meat dishes, and scrumptious sweet treats.But this is not just a recipe book. Rob explains how to use these dishes to target your health needs, whether that's losing weight, gaining energy, getting clearer skin – or just having a weekend detox after a few days of indulgence. Eat your way to glowing health with this smart new guide to daily wellbeing.
Beat the BBQ Bloat: How to Have a Healthy BBQ!
Are You Ready for the City Strongman Workout?


She was accused of stealing items from the 7-Eleven store on Cranberry Highway in East Wareham. She faces arraignment in Wareham District Court.
Police also arrested a Plympton man following a weekend disturbance in Wareham. Police responded to a report of an incident on Claudia Street Sunday afternoon around 2:45.
Thirty-year-old John Guilford Jr. of Plympton was arrested on several charges, including disorderly conduct, disturbing the peace, and threats to commit a crime.