Philips is finally back with a new Android Smartphone. While many the companies introducing new Smartphones, the electronic manufacturer was just watching all things happening in the market, but finally it is back to try its luck in the Smartphone market with an Android Smartphone. The device named W732 is an entry-level Smartphone with a neat form-factor, wide display and better battery life.
The Philips W732 Droid is powered by GHz single-core MediaTek MT6575 processor and features a 4.3-inch LCD IPS display with a 480×800 pixels resolution, 1GB of RAM, a 5MP rear camera and a juicy battery, 2,400mAh that will offer you 13 hours of continuous talk time or 10.5 hours of web browsing on a single charge.
The Philips W732 Droid also features Wi-Fi, Bluetooth and GPS on board for connectivity along with two SIM slots. Philips W732 Droid measures 126.37 x 67.44 x 12.3 mm, and weighs 166 grams. The Smartphone runs on Android 4.0 ICS.
The Philips W732 Droid will debut in China and Russia in the coming weeks and will be available in black, white, pink, blue and green colors. Unfortunately, pricing and global availability are yet to be announced, but stay tuned, we will keep you posted.
Next story Samsung Galaxy Note 2 to debut on August 30?


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Rightly Divide the Word of Truth
2023-Q1-01 Lesson Review: Part of God's Family
December 31, 2022 Andrew S. Baker (ASB) Season 231 Episode 1
Dec 31, 2022 Season 231 Episode 1
Andrew S. Baker (ASB)
Part of God's Family — Review of Lesson #1 of the 1st Quarter of 2023 -
The Sabbath School Lesson study guide can be found here:
https://ssnet.org/lessons/23a/less01m.html
The title of this quarter's theme is: Managing for the Master – Till He Comes
For the next 13 weeks (January to March 2023), we will take a look at the practical implications of occupying this planet in a meaningful and productive way while we wait for the Second Coming.
If you have any questions or comments, please send them to: BibleQuestions@ASBzone.com
Christ, Humanity and Salvation
Revelations of the Godhead (at TrueWisdom)
The Thing About Tithing (at TrueWisdom)
Tithing Q&A (at TrueWisdom)
During many of our podcasts, you will hear us make reference to "The Key Principles of Effective Bible Study," a document which outlines core concepts shown in the scriptures that will help you better understand many Biblical themes and doctrines. We have done a whole podcast series on these principles which can be found here (https://BibleStudy.ASBzone.com/357512/8572886).
Lastly, we recommend that you check out https://TrueWisdom.buzzsprout.com for additional Bible Study podcasts, covering many different Bible topics, and done in a slightly different format from the podcasts on this channel.
We pray that all of these resources will be very helpful to you in your Bible Studies.
All content © 2023 ASBzone: Rightly Divide the Word of Truth.

redpajama_set_name: RedPajamaCommonCrawl


Earlier I gave an on camera interview with the director of the American Bible Society's GOD SETS YOU FREE Program. I was able to share my story of hope with him, as well as answer the many questions he posed about what it is like to serve God while in prison, and what if any positive affects prison ministries have on those who are locked up.
In addition, with God's guidance, I presented a written message that the American Bible Society plans to include in their upcoming reprinted edition of the GOD SETS YOU FREE Bible that is due to be released this summer. The Bible will be distributed for free to prisons, jails and juvenile detention facilities throughout the United States.
The title of my message is, "Living with Hope." And my hope is that every man or woman who gets a copy of this Bible will read my words and then give careful consideration to the Lord Jesus Christ. I hope, too, that the Holy Spirit will draw those to Christ who need salvation.


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Nova Scotia assumes people want to donate organs after death
Nova Scotia ranked first in North America regarding presumed consent for organ donation.
Nearly a year after NS became the first jurisdiction in North America to assume residents agree to donate their organs if they die, it is difficult to gauge the program's popularity.
Everyone in Nova Scotia is presumed to have given consent to having their organs harvested and donated under the Human Organ and Tissue Donation Act. Which went into effect on Jan. 18, unless they opted out of the program while they were alive. In contrast, the rest of the continent requires people to opt-in.
Dr. Stephen Beed, medical director of the province's organ and tissue donation program, said the COVID-19 pandemic shut down his department between May and June, causing staff to be diverted to other areas of the health system.
"It's going to be hard to conclude anything in a year like this," Beed said recently, adding that he suspected the year-end numbers on donation rates were skewed. However, he stated that predicting how much by how much is difficult.
donate organs after death; Image from The Hill
Nonetheless, he expressed optimism because preliminary referral numbers were "significantly higher" earlier this year. "When we first started this, we had donor numbers in the high teens, and we wanted to get to the mid-to high-twenties," Beed explained. "I certainly hope that's where we're going to be as a new norm, if not higher."
According to Beed, the most recent figures show that approximately 50,000 people or about 5% of the province's population have opted out so far. "We do know from Canadian survey data that the percentage of people who say they want to opt-out is greater than 5%," Beed explained.
He also wondered if more public education is needed, given the number of people who have called the organ donation program to find out if they have to opt out. Many people, he claims, mistakenly believe they are not medically qualified to be donors.
"People are opting out for reasons we believe warrant more education," he said. "It's not that they disagree with the approach; it's that there are some misconceptions out there."
Get Nova scotia and Canada's top News, Latest News, and other News of the world only at the most trustable news website of Canada Novascotiajournal.com
Tags: death, donate organs after death, Dr. Stephen Beed, featured, Nova Scotia news, people want to donate organs
Unimaru art museum located between North and South Korea
Scientists are working on chewing gum that could help cut COVID-19 transmission
COVID-19 vaccinations for child aged 5 to 11 will begin in N.S. on December 2
The rainstorm is expected to hit eastern Nova Scotia and Cape Breton
Remembrance Day ceremonies in N.S. with COVID-19 protocols
N.S. reports 394 new cases of COVID-19, with 7 people hospitalized
On Nova Scotia's south shore, a historic land transfer has happened
The Bargain shop is return to new Waterford, Nova Scotia


text:
Patent application title: Pineapple plant named Rose (EF2-114)
Inventors: Ebrahim Firoozabady (Coral Gables, FL, US) Thomas R. Young (Coral Gables, FL, US)
Assignees: Del Monte Fresh Produce Company
IPC8 Class: AA01H500FI
USPC Class: PLT156
A new pineapple variety named `Rose` is provided. Internal red or pink color with unique shell morphology and possibility of flowering control trait are traits of the new variety.
1. A new and distinct variety of Ananas comosus plant named `Rose` with breeder name EF2-114 as shown and described herein.
SPECIES NAME
[0001] Ananas comosus.
VARIETY DENOMINATION
[0002] `Rose`, with breeder name EF2-114.
[0003] A new variety of pineapple (Ananas comosus), family Bromeliaceae, has been developed using genetic engineering techniques and named `Rose`. This process took 6 years; started in August 2005, using crown materials from variety MD2 (also known as Del Monte Gold pineapple), imported from Hawaii, to produce in vitro shoot cultures, introduce genes and DNA elements into leaf base sections, regenerate complete plants, perform field trials and select plants with internal pink-or red-colored fruits. The selected plants were asexually propagated in the field and via meristem culture to confirm the colored traits and other traits related to fruit and agronomic performance.
[0004] The plant is very similar to parental line, MD2, for plant and fruit characteristics and fruit internal quality. However, the internal flesh color in Rose is pink or red, due to accumulation of lycopene in the edible part of fruit, the shell morphology is referred as "Tiger" and it might be tolerant to natural occurrence of flowering. This new variety is best suited for the fresh market and residual fruit may be processed as juice or frozen product.
[0005] The main objective of this invention was to produce a unique and differentiated variety of pineapple by accumulation of high levels of carotenoids, in particular lycopene that produces red internal color while retaining most of the characteristics of the parental line, MD2.
[0006] The invention relates to carotenoid biosynthesis in pineapple plants. More specifically, this novel pineapple was produced by genetically transforming MD2 cells in tissue culture and regenerating complete plants (Scheme 1) with expression regulators that modulate lycopene biosynthesis in the internal section of the fruit. In addition plants were transformed with genes involved in ethylene biosynthesis pathway to control flowering in the plants.
[0007] Carotenoids are isoprenoid molecules that are widespread in nature and can occur as pigments in fruits, flowers, birds, and crustaceans. Animals are unable to synthesize carotenoids de novo, and rely upon the diet as a source of these compounds. Carotenoids may contribute fundamentally to human health and in recent years there has been considerable interest in dietary carotenoids with respect to their potential in alleviating age-related diseases in humans. This attention has been mirrored by significant advances in cloning most of the carotenoid genes and in the genetic manipulation of crop plants with the intention of increasing levels in the diet.
[0008] In plants, carotenoids are essential components of the photosynthetic apparatus and are responsible for the red, orange, and yellow color of many flowers and fruit. Our understanding of carotenoid biosynthesis has advanced dramatically in recent years (Hirschberg, 2001; Fraser and Bramley, 2004). The pathway involves a series of desaturations, cyclizations, hydroxylations, and epoxidations commencing with the formulation of phytoene (See FIG. 1). A subsequent series of desaturations is responsible for lycopene synthesis. After the desaturation reactions, the cyclization of lycopene is catalyzed by two enzymes, the beta-cyclase and the zeta-cyclase, leading to the formation of beta-carotene (two beta-rings) and alpha-carotene (one beta-ring and one zeta-ring) (Cunnigham et al., 1996, 1998).
[0009] The genes of interest are derived from edible plant species, pineapple (Ananas comosus) or tangerine (Citrus reticulata). Specifically, we have over-expressed a tangerine phytoene synthase gene (Psy) (Ikoma et al, 2001). The gene is under transcriptional control of the pineapple bromelain inhibitor (BRI) gene. We have also suppressed endogenous lycopene β-cyclase (b-Lyc) and/or lycopene ε-cyclase (e-Lyc) gene expression using RNA interference (RNAi) technology in order to increase accumulation of lycopene in edible tissues of pineapple fruit (Young, T. and Firoozabady, E. 2010, U.S. Pat. No. 7,663,021). We constructed sense- and antisense-oriented sequences of the b-Lyc and e-Lyc genes derived from pineapple, which are separated by an intron of the light-inducible tissue-specific LS1 gene derived from potato (Solanum tuberosum) to form a hairpin structure. These genes are under transcriptional control of the Bromelain inhibitor gene promoter, which drives strong fruit-enhanced expression of the RNAi construct (Wintz, H-C and Firoozabady, E. 2005).
[0010] Flower initiation in pineapple can occur naturally primarily due to cool temperatures and short days. Natural flowering of pineapple plants is a major industry problem. To achieve the controlled flowering trait, we have altered expression of genes involved in ethylene biosynthesis. Ethylene is a plant hormone that plays an important role in every phase of plant development, including seed germination, fruit ripening, leaf and flower senescence, and abscission. In plants, ethylene is synthesized from the amino acid, Methionine. The immediate precursor of ethylene in higher plants is 1-aminocyclopropane-1 carboxylic acid (ACC) (Adams and Yang, 1979).
[0011] Ethylene is known to inhibit flowering in most plants. In mango and pineapple, ethylene promotes flowering (Salisbury and Ross, 1992). Ethylene, ethylene producing compounds and auxins have been used to induce flowering in commercial pineapple production (Turnbull et al., 1993).
[0012] We isolated a meristem-specific ACC synthase (flACCS) gene from pineapple and constructed sense- and antisense oriented sequences of the ACC synthase gene, which are separated by an intron of the light-inducible tissue-specific LS1 gene derived from potato (Solanum tuberosum) to form a hairpin structure for RNAi suppression of endogenous ACC synthase. The RNAi construct is under transcriptional control of the meristem-specific ACC promoter derived from pineapple.
TRANSFORMATION METHOD
[0013] Pineapple was transformed by Agrobacterium tumefaciens-mediated transformation of organogenic tissues using a method described by Firoozabady (U.S. Pat. No. 8,049,067). To achieve both high-carotenoid and controlled flowering phenotypes, Agrobacterium strains containing either a transformation plasmid for increased carotenoid biosynthesis or for decreased ethylene biosynthesis were co-cultivated with recipient pineapple tissues. Putative transformed tissues were selected on media containing chlorsulfuron and subsequently screened for the presence of target genes by PCR.
[0014] A. tumefaciens, [Strain GV31011] (Koncz and Schell, 1986), is a disarmed Agrobacterium strain commonly used for the delivery of T-DNA into plant cells. Different genes were inserted into T-DNA in a binary vector (see FIG. 2) for introduction to pineapple. The Psy derived from Citrus reticulata, tangerine (Ikomaa et al, 2001), encodes an enzyme that coverts geranylgeranyl pyrophosphate (GGPP) to cis-phytoene, an intermediate in lycopene and beta-carotene biosynthesis.
[0015] The lycopene beta-cyclase gene (b-Lyc) derived from Ananas comosus, pineapple, encodes an enzyme that coverts lycopene to gamma-carotene, a metabolic precursor of beta-carotene.
[0016] The lycopene epsilon-cyclase gene (e-Lyc) derived from Ananas comosus, pineapple, encodes an enzyme that coverts lycopene to sigma-carotene, a metabolic precursor of alpha-carotene.
[0017] The modified acetolactate synthase (Chaleff, R. S., and Mauvais, C. J., 1984) (ALS) gene (surBHRA) derived from Nicotiana tabacum, tobacco, catalyzes the biosynthesis of branched chain amino acids even in the presence of chlorsulfuron (Lee, K. et al., 1988), which allows for the selection of transformed pineapple cells.
[0018] Plasmid pHCW1 used for pineapple transformations was constructed by the laboratory of Del Monte Fresh Produce Company, Richmond, Calif. pHCW1 contains a tetracycline resistance gene (tetRA) from plasmid RP1 and the origin of replication from plasmid pACYC, which allows for selection and maintenance in Escherichia coli and the pVS1 replicon derived from Pseudomonas aeruginosa, which ensures replication in Agrobacterium tumefaciens. pHCW1 contains the 25-base pair sequences that delimit the T-DNA transfer and a 110-base pair synthetic sequence between the borders that forms multiple cloning restriction sites to allow integration of different T-DNA cassettes (see Table 1).
[0019] The plasmid pCHW1 was used to create pHCW.T-7 and pHCWflACC3'-2 binary vector plasmids. Binary vectors were transferred to disarmed A. tumefaciens strain GV3101. The GV3101 with pHCW.T-7 vector was named AG76 and the one with pHCWflACC3'-2 was named AG62 (see FIG. 3). AG76 and AG62 were mixed together and used for pineapple transformation. Genetic elements of the vectors are described in Tables 2 and 3.
[0020] Genetic engineering of the MD2 took place in the Laboratory of Del Monte Fresh Produce Company in Richmond, Calif., USA, where transgenic plants were produced and propagated in tissue culture. Then the propaguls were taken to the research area of Corporacion de Desarrollo Agricola Del Monte, S.A. (Pindeco), Buenos Aires-Puntarenas, Costa Rica, for field evaluation, propagation in the field and in the laboratory for mass propagation of the variety.
[0021] A new variety of pineapple (Ananas comosus), family Bromeliaceae, has been developed using genetic engineering techniques and named `Rose` or EF2-114. Using crown materials from variety MD2 (also known as Del Monte Gold pineapple) to produce in vitro shoot cultures, introduce genes and DNA elements into leaf base sections, regenerate complete plants, perform field trials and select plants with internal pink- or red-colored fruits. The selected plants were asexually propagated in the field and via meristem culture to confirm the colored traits and other traits related to fruit and agronomic performance. The invention relates to production of a new and distinct variety of the Bromeliaceae, or pineapple family.
[0022] The new plant variety EF2-114 is characterized by pink or red flesh color and "Tiger" shell color, when compared with the parental line, MD2, and it might be tolerant to natural occurrence of flowering.
[0023] Internal color of fruits can be variable depending on the stage of ripening (FIG. 4). The tiger trait was defined as the color in shell has in the shoulder of each fruitlet a combination of colors green, yellow, orange (and red) due to expression of carotenoid genes in the shell (FIGS. 5-7). The plant morphology generally is the same as MD2, the parental plant (FIGS. 8-11).
BOTANICAL DESCRIPTION OF THE PLANT
[0024] The description of the new variety is based on observations of well fertilized specimens which were grown under field conditions, in the Buenos Aires region, Costa Rica, where temperatures generally range from 14° C. to 37° C., and annual rainfall averages 3251 mm.
[0025] The plants were grown at a research facility in Buenos Aires-Puntarenas, Costa Rica.
[0026] All fruit and plant characteristics including color terminology and color designations reported herein are in accordance with Brazilian Descriptors for MD2 (Table 4). Essentially, "Rose" is same as MD2 for all fruit and plant characteristics with the exception of fruit internal color, Tiger trait and possibly flowering control trait. See attached Brazilian Descriptors for MD2.
[0027] Plant identification:
[0028] Name.--Ananas comosus.
[0029] Parentage.--MD2.
[0030] Origin.--Genetic engineering of MD2 followed by selection in the field trials for the traits of interest.
[0031] Botanic.--Bromeliaceae or pineapple family. Subfamily: Bromelioideae. Genus: Ananas. Subgenus: comosus. Variety: "Rose", breeder's name EF2-114.
[0032] Commercial.--Bromeliad fruit plant.
[0033] General:
[0034] Fertility.--As any other grown up pineapple, this plant is self-incompatible. For this reason, seeds are rarely observed. Commercial propagation is via vegetative propagules (suckers, stem shoots and slips) and fruit crowns.
[0035] Vigor.--It is considered that the plant vigor is similar as to mother plants, MD2.
[0036] Yield.--Estimated yield is same as MD2, 120-125 MT/ha in Plant Crop and 95-100 MT/ha in Ratoon Crop.
[0037] Market.--Fruit will be designated to the international fruit market and commercialized into the fresh fruit market. Residual fruit may be processed as juice or frozen product.
[0038] 1. Adams D. O., Yang S. F. 1979. Ethylene biosynthesis: Identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc Natl Acad Sci U S A.; 76(1):170-174.
[0039] 2. Barker, R., Idler, K., Thompson, D., Kemp, J. (1983) Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol. Biol. 2:335-350.
[0040] 3. Chaleff, R. S., and Mauvais, C. J. (1984) Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443-1445.
[0041] 4. Chang, A. C. Y. & Cohen, S. N. (1978). Construction and characterisation of amplifiable multicopy DNA cloning vehicles derived from the p15a cryptic miniplasmid. J Bacteriol 134, 1141-1156
[0042] 5. Cunningham, F. X., Pogson, B. J., Sun, Z., McDonald, K., DellaPenna, D., and Gantt, E. (1996). Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8, 1613-1626.
[0043] 6. Cunningham, F. X., Jr., and Gantt, E. (1998). Genes and enzymes of carotenoid biosynthesis in plants. Arum. Rev. Plant Physiol. Plant Mol. Biol. 49,557-583.
[0044] 7. DePicker, A., Stachel, S., Dhaese, P., Zambryski, P., Goodman, H. M. (1983) Nucleotide sequences and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene. J. Mol. Appl. Genet. 1:499-511.
[0045] 8. Eckes, P., Rosahl, S., Schell, J. and Willmitzer, L., (1986) Isolation and characterization of a light-inducible, organ-specific gene from potato and analysis of its expression after tagging and transfer into tobacco and potato shoots, Mol. Gen. Genet. 205,14-22
[0046] 9. Firoozabady, E. U.S. Pat. No. 8,049,067, Organogenic transformation and regeneration.
[0047] 10. Fraser, P. D., & Bramley, P. M. (2004). The biosynthesis and nutritional uses of carotenoids. Progress in lipid research, 43(3), 228-65
[0048] 11. Hirschberg, J. (2001). Carotenoid biosynthesis in flowering plants. Curr. Opin. Plant Biol. 4,210-218.
[0049] 12. Hirschberg, I, M. Cohen, M. Harker, T. Lota, V. Mann, and I. Pecker. 1997. Molecular genetics of carotenoid biosynthesis pathway in plants and algae. Pure Appl. Chem. 69:2145-2150.
[0050] 13.
[0051] 14. Ikomaa, Yoshinori, Akira Komatsub, Masayuki Kitac, Kazunori Ogawad, Mitsuo Omurac, Masamichi Yanoc and Takaya Moriguchi, (2001) Expression of a phytoene synthase gene and characteristic carotenoid accumulation during citrus fruit development. Physiologia Plantarum 111: 232-238.
[0052] 15. Itoh, Y., Watson, J. M., Haas, D., Leisinger, T. (1984) Genetic and molecular characterization of the Pseudomonas plasmid pVS1. Plasmid 11:206-220.
[0053] 16. Koncz C, and Schell J. (1986) The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204: 383-396
[0054] 17. Lee, K. et al., (1988) The molecular basis for sulfonylurea herbicide resistance in tobacco. EMBO J. 7:1241-1248.
[0055] 18. Neuteboom L. W., Kunimitsu W. Y., Webb D, Christopher D. A., 2002. Characterization and tissue-regulated expression of genes involved in pineapple (Ananas comosus L.) root development. Plant Science, 165:1021-1035.
[0056] 19. Salisbury, F. B. and Ross, C. W. 1992. Plant Physiology, 4th ed., Wadsworth.
[0057] 20. TURNBULL, C. G. N.; NISSEN, R. J.; SINCLAIR, E. R.; ANDERSON, K. L.; SHORTER, A. J. Ethephon and causes of flowering failure in pineapple. Acta Horticulturae, Honolulu, n.334, p. 83-88, out, 1993.
[0058] 21. Young, T. and Firoozabady, E. 2010, U.S. Pat. No. 7,663,021. Transgenic pineapple plants with modified carotenoid levels and methods of their production
[0059] 22. Waters, S. H., Rogowski, P., Ginsted, J., Altenbuchner, J., and Schmidt, R. (1983) The tetracycline resistance determinants of RP1 and Tnl 721; nucleotide sequence analysis. Nuc. Acids Res. 11(17):6089-6105.
[0060] 23. Wintz, H-C and Firoozabady, E. 2005, patent PUBLICATION NUMBER-2006113709/WO-A2 Plant promoters, terminators, genes, vectors and related transformed plants.
FIGURE LEGENDS
[0061] FIG. 1. Enzymes and genes in the carotenoid biosynthesis pathway in plants and algae. (Adapted from J. Hirschberg et al., Pure & Appl. Chem. 69(10):2151-58). Genes used in pineapple transformations to create EF2-114 are shown in the boxes. Psy is phytoene synthase gene from tangerine and b-lyc and e-Lyc are partial sequences of pineapple lycopene β-cyclase and lycopene ε-cyclase genes respectively.
[0062] FIG. 2. Map of plasmid binary vector backbone, pHCW1, used for genetic engineering of pineapple. For definition of genetic elements see Table 1.
[0063] RB--Right Border
[0064] pVS 1--Agrobacterium origin of replication
[0065] TetR/A--Tetracyclin gene (bacterial selectable marker)
[0066] pACYC--Bacterial origin of replication
[0067] LB--Left Border
[0068] FIG. 3. Plasmid pHCW1 was used to create pHCW.T-7 binary vector (in AG76) and pHCWflACC3'-2 binary vector (in AG62). For definition of genetic elements see Table 2 and 3.
[0069] Vector Backbone:
[0070] RB: Right T-DNA Border
[0071] pVS1: Agrobacterium origin of replication
[0072] TetR/A: Tetracyclin gene (bacterial selectable marker)
[0073] pACYC: Bacterial origin of replication
[0074] LB: Left T-DNA Border
[0075] T-DNA
[0076] ALS cassette: EHS-Ubp (promoter)--ALS-ALS3' terminator
[0077] Psy casssette: BRIP (promoter)-Psy-Ubp terminator
[0078] bLyc cassette: BRIP (promoter)-bLyc (+)-LS 1 intron-bLyc(-)-Ubp terminator
[0079] eLyc cassette: BRIP (promoter)-eLyc(+)-LS 1 intron-eLyc(-)-Ubp terminator
[0080] flACS cassette: Ubp(promoter)-flACS(+)-LS1 intron-flACS(-)-Ubp terminator
[0081] FIG. 4. Shows the internal color of the fruits being pink to red due to accumulation of lycopene.
[0082] FIG. 5. Shows "Tiger" shell color in EF2-114 fruits. The color in shell has in the shoulder of each fruitlet a combination of colors green, yellow, orange (and red).
[0083] FIG. 6. A "Tiger" fruit showing shell and internal color at harvest.
[0084] FIG. 7. Shows an immature fruit of `Rose` with Tiger shell morphology (left) and an immature fruit of MD2 (right) both ˜145 days after forcing.
[0085] FIG. 8. Shows overhead view of `Rose` EF2-114 plant. FIG. 9. Shows a `Rose` EF2-114 plantation. FIG. 10. Shows EF2-114 (left) and MD2 (right) experimental plots. FIG. 11. Shows EF2-114 (left) and MD2 (right) meristem culture-derived plants. After 15 weeks growth in greenhouse, ˜15 cm long plants were transplanted in pots, and the pictures were taken 11 months later.
TABLE-US-00001 TABLE 1 Genetic Elements of plasmid pHCW1 Genetic Element Source and Function RB A 25 bp nucleotide sequence that acts as the initial point of DNA transfer into plant cells which was originally isolated from pTiT37 (Depicker et al., 1982). pvs1 The pVS1 replicon derived from Pseudomonas aeruginosa, which ensures replication in Agrobacterium tumefaciens (Itoh et al., 1984). TetRA A tetracycline resistance gene from plasmid RP1, which allows for selection of the binary plasmid in Agrobacterium tumefaciens and Escherichia coli (Waters et al., 1983). pACYC The origin of replication from plasmid pACYC, which ensures replication in Escherichia coli (Chang et al., 1979). LB A 25-nucleotide sequence that delimits the T-DNA transfer and acts as the endpoint of DNA transfer into plant cells. It was originally isolated from TiA6 (Barker et al., 1983). MCS A 110-nucleotide sequence that synthetically was created, which is composing of multiple cloning restriction sites in order to allow integration of different cassettes into the plasmid.
TABLE-US-00002 TABLE 2 Genetic Elements of T-DNA in plasmid pHCW.T-7 in AG76 Genetic Size Element (Kb) Function (Reference) BRIp2.5 2.5 A promoter derived from the bromelain inhibitor (BRI) gene from Ananas comosus that drives fruit- enhanced expressionof the target gene(s). Psy 1.131 A phytoene synthase (Psy) gene from Citrus reticulata, identical to the gene isolated from mandarin fruit (Ikoma et al. 2001), which encodes an enzyme in carotenoid biosynthesis. Ubpter 0.94 A terminator derived from the polyadenylation sequence of the ubiquitin (Ubi) gene from Ananas comosus terminates transcription of the transgene(s). BRIp2.5 2.5 A promoter derived from the promoter sequence from the bromelain inhibitor (BRI) gene from Ananas comosus that drives fruit-enhanced expression of the RNAi transgene. eLyc sense 0.504 A fragment of the lycopene ε-cyclase gene from fragment Ananas comosus in sense orientation, which is used in an RNAi expression system to down-regulate endogenous lycopene ε-cyclase, an enzyme in carotenoid biosynthesis. ST-LS1 0.193 An intron of the light-inducible tissue-specific ST-LS1 gene from Solanum tuberosum that functions as a spacer between sense and antisense gene fragments enhancing vector stability. eLyc 0.504 A fragment of the lycopene ε-cyclase gene from antisense Ananas comosus in antisense orientation, which is fragment used in an RNAi expression system to down- regulate endogenous lycopene ε-cyclase, an enzyme in carotenoid biosynthesis. Ubpter 0.94 A terminator derived from the polyadenylation sequence of the ubiquitin (Ubi) gene from Ananas comosus terminates transcription of the RNAi transgene. BRIp2.5 2.5 A promoter derived from the promoter sequence from a bromelain inhibitor (BRI) gene from Ananas comosus that drives fruit-enhanced expression of the RNAi transgene. b-Lyc 0.619 A fragment of the lycopene β-cyclase gene from sense Ananas comosus in sense orientation, which is used fragment in an RNAi expression system to down-regulated endogenous lycopene β-cyclase, an enzyme in carotenoid biosynthesis. ST-LS1 0.193 An intron of the light-inducible tissue-specific ST-LS1 gene from Solanum tuberosum (Eckes et al, 1986) that functions as a spacer between sense and antisense gene fragments enhancing vector stability. b-Lyc 0.619 A fragment of the lycopene β-cyclase gene from antisense Ananas comosus in antisense orientation, which fragment is used in an RNAi expression system to down- regulated endogenous lycopene β-cyclase, an enzyme in carotenoid biosynthesis. Ubpter 0.94 A terminator derived from the polyadenylation sequence of the ubiquitin (Ubip) gene from Ananas comosus terminates transcription of the RNAi transgene. Selectable Marker EHS1.7- 4.257 A promoter derived from the epoxide hydrolase Ubp1.5 (EHS) gene fused to the ubiquitin (Ubip) gene promoter and the native intron from Ananas comosus that drives constitutive expression of the selectable marker gene. EHS1.7 1.7 A promoter derived from the epoxide hydrolase (EHS) gene from Ananas comosus (Neuteboom et al, 2002) that drives constitutive expression of the selectable marker gene. Ubp1.5 1.5 + A 1.5 kb tetrameric ubiquitin gene promoter from 1.057 pineapple (Ananas comosus), which drives constitutive expression of the selectable marker gene. The promoter includes an endogenous 1057 bp intron sequence. ALS 1.17 A mutant acetolactate synthase gene from tobacco (Nicotiana tabacum), which confers resistance to chlorsulfuron and allows selection of transformed plant cells (Chaleff and Mauvais, 1984; Lee et al., 1988). ALS 3' 2.7 An endogenous terminator derived from untrans- lated polyadenylation signal of the acetolactate synthase (ALS) gene from Nicotiana tabacum (Chaleff and Mauvais, 1984; Lee et al., 1988).
TABLE-US-00003 TABLE 3 Genetic Elements of T-DNA in plasmid pHCWflACC3'-2 in AG62. Elements are the same as above (Table 2) except those mentioned below. Genetic Size Element (Kb) Function (Reference) flACS(+)sense 0.406 A 406 bp 3'sequence of meristem-specific ACC synthase gene, isolated from pineapple (A. comosus, in the sense orientation, which is used in an RNAi expression system to down- regulate endogenous ACC synthase, a key enzyme in ethylene biosynthesis, thereby producing a plant that displays improved characteristics such as delayed flowering. flACS(-) 0.406 A 406 bp 3'sequence of meristem-specific ACC antisense synthase gene, isolated from pineapple (A. comosus), in the antisense orientation.
TABLE-US-00004 Scheme 1: production of EF2-114 pineapples. Stage Date (time) Immature MD2 fruits (40 days prior to harvest) Aug. 10, 2005 arrived from Hawaii Meristems isolated from the crown and cultured Aug. 17, 2005 Established shoot culture and propagate in vitro Aug. 17, 2005 to Sep. 20, 2006 Longitudinal sections of shoots were prepared and Sep. 20, 2006 to Sep. cultured 29, 2009 Leaf base sections were prepared and mixed with Sep. 29, 2006 to Oct. Agrobacterium strains AG-62 and AG-76 and 02, 2006 cocultivated for three days Leaf bases were transferred to recovery media Oct. 2, 2006 to Oct. containing carbenicillin (to kill off 27, 2006 Agrobacterium) Leaf bases were transferred to selection media Oct. 27, 2006, Nov. containing antibiotics (to kill off residual 2, 2006, Nov. 14, 2006, Agrobacterium) and chlorsulfuron, CS (an Dec. 4, 2006, Jan. 3, herbicide), to select for transformed plant cells 2007, Jan. 29, 2007, and produce transformed organogenic materials. Feb. 15, 2007 A total of 7 transfers were done with increasing selection pressure to produce pure transgenic tiny buds. Tiny bud clusters were transferred (two times) to Mar. 13, 2007, Mar. media containing CS and antibiotics to further 29, 2007 grow and multiply transformed shoots and shoot buds Shoots and shoot buds were transferred to media Apr. 10, 2007 containing high level of Agar (8 and 10 g/l) to normalize transformed shoots and shoot buds Shoot clusters were transferred to media with CS, May 4, 2007 to Dec. antibiotics, Agar (7-10) and hormones to 14, 2007 normalize and propagate shoots. An event referred to as 16.184.1 included 14 lines Jan. 21, 2008 were subcultured in liquid media for further growth 9 lines or groups of the 16.184.1 event (consisting Feb. 14, 2008 of 349 shoots) were shipped to Pindeco for field trials. In Pindeco the 9 groups were numbered as 109-117 and were grown in the greenhouse for further growth and filed trials. 2 additional lines or groups of the 16.184.1 event Aug. 1, 2008 (consisting of 85 shoots) were shipped to Pindeco for field trials. In Pindeco the 2 groups were numbered as 208 and 209 and were grown in the greenhouse for further growth and filed trials. PCR analyses confirmed that the genes are Aug. 26, 2008 present in tissue culture-derived event shoot samples 2 additional lines or groups of the 16.184.1 event Jan. 26, 2009 (consisting of 150 shoots) were shipped to Pindeco for field trials. In Pindeco the 2 groups were numbered as 345 and 346, and were grown in the greenhouse for further growth and filed trials. Plants were grown in the GH for 15-20 weeks 2009-2012 before transferred to soil in the field trials. Fruits were cut for internal color evaluation. During the field trials groups 109, 110, 114, 116, 208, and 346 exhibited pink or red internal colors. Internal quality (Brix, citric acid, ascorbic acid and pH) were measured on the colored fruits and propaguls were grown for future generations' evaluation. Molecular analyses (PCR and Southern) confirmed the presence of the genes. Southern further confirmed that all these group are one transgenic even and collectively are referred as EF2-114
TABLE-US-00005 TABLE 4 MINIMUM DESCRIPTORS FOR PINEAPPLE (Ananas comosus (L.) Merrill) Proposed name for the plant variety MD-2 Code for each Description of the description Characteristic characteristic MD-2 1. Plant: attitude semi-erect (+) intermediary open 7 2. Plant: number of active low leaves medium 5 (+) high 3. Leaf: length short (+) medium 5 long 4. Leaf: width narrow (+) medium 5 broad 5. Leaf predominant color on light green upper face dark green 2 purple-green green purple purple vred 6. Leaf: anthocyanin coloration absent 1 present 7. Leaf: variegation absent 1 present 8. Leaf: spines absent (+) inconspicuos 2 conspicuos 9. Leaf: distribution of spines at at base only margin at apex only 2 (+) at base and apex regular irregular 10. Inflorescense: number of low flowers medium 5 (+) high 11. Flower: predominant whitish coloration of sepal greenish purplish 3 12. Flower: petals base free 1 fused 13. Flower: imbricate petals absent present 2 14. Flower: predominant whitish coloration of petal tip light purple medium purple 5 dark purple 15. Flower: ratio of white color low 3 at petal medium (+) high 16. Flower: disposition of anthers separate grouped 2 17. Flower: number of pollen at low 3 the anthers medium high 18. Flower: length of style short (+) medium 2 long 19. Suckers: number low (+) medium 5 high 20. Peduncle: length short 3 (+) medium long 21. Peduncle: diameter at the small middle portion medium 5 (+) large 22. Peduncle: number of (absent or very low) younglets/bulbs/slips (low) (+) (medium) 3 (high) (very high) 23. Peduncle: number of bracts low medium 5 high 24. Peduncle: imbricate bracts absent present 2 25. Peduncle: trichomes absent present 2 26. Fruit: length short (+) medium 5 long 27. Fruit: diameter at base small (+) medium large 7 28. Fruit: diameter at the middle small portion medium large 7 29. Fruit: diameter of tip small (+) medium large 7 30. Fruit: shape conic (+) conic to cylindric cylindric 3 eliptic global/spheric 31. Fruit: color before silvery green physiologic maturity, with light green the fruit completely shaped medium green dark green 4 red r purple 32. Fruit: color of skin at the (green) point to consume (green with yellow spots) (light yellow) (golden yellow) (redish orange) 4 (purple) 33. Fruit: color homogeneity of absent the skin present 2 34. Fruit: number of fruit basal (absent or very low) 1 slips (low) (+) (medium) (high) (very high) 35. Fruit: detachable fruitlets absent 1 present 36. Fruit: relief of fruitlet flat 1 prominent very prominent 37. Fruit: color of flesh white to cream yellow golden yellow 3 orange 38. Fruit: firmness of flesh soft medium firm 7 39. Fruit: fibrousness in the flesh low medium 5 high 40. Fruit: succulence low medium 5 high 41. Fruit: diameter of central small axis medium (+) large 7 42. Fruit: concentration of low soluble solids (Brix degrees) medium 5 (+) high 43. Fruit: acidity (fixed in low percentage) medium 5 (+) high 44. Fruit: flavor weak medium 5 strong 45. Crown: position erect open 3 decumbent 46. Crown: length short (+) medium 5 long 47. Crown: weight low (+) medium high 7 48. Crown: tendency to multiple (absent or very low) crowns (low) (medium) 3 (high) (very high) COMPLEMENTARY CHARACTERISTIC 49. Resistance to fusarium (high resistance) (Fusarium subglutinans) (resistant) (medium resistance) (medium susceptibility) (susceptible) (high susceptibility) 4 (+) See item "VI. OBSERVATIONS AND FIGURES".
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Less than a 10–minute walk from the beach, Tomić is located in a green area 4 km off Vir. Visitors can sit back on the furnished patio and make use of Barbecue amenities. The apartment presents a kitchenette and living area with sofa bed. The toilet comes with a shower. Tomić is 10 km from Privlaka. Zadar is obtainable in 40 minutes by automobile.
Apartments Štefani ~0,1 km, Vir price per day from € 54 Set 550 metres from a beach with a bar, Apartments Štefani is set 5 km from the city of Vir. It presents air-cooled apartments with a furnished balcony or an outdoors terrace. All apartments comprise satellite television and a kitchen with a dining area. Featuring a shower, private restrooms also come with free toiletries and towels.
Apartments Andrea ~0,5 km, Vir price per day from € 40 Situated in Vir, Apartments Andrea presents free bicycles. The beach with a bar is obtainable within 800 metres and the closest grocery store is positioned 150 metres away. This self-catering accommodation presents gratis wireless internet. The apartment will provide you with a TV and an outdoors terrace. There is a full kitchen with a fridge and kitchenware. At Apartments Andrea you will find grill amenities.
Tomić ~0.1 km, Vir price per day from € 42 Less than a 10–minute walk from the beach, Tomić is located in a green area 4 km off Vir. Visitors can sit back on the furnished patio and make use of Barbecue amenities. The apartment presents a kitchenette and living area with sofa bed. The toilet comes with a shower. Tomić is 10 km from Privlaka. Zadar is obtainable in 40 minutes by automobile.
Privlaka ( ~ 6.7 km ): 157+ accommodation units.
Sabunike ( ~ 9.2 km ): 54+ apartments.
Nin ( ~ 12 km ): 200+ apartments.
Zaton ( ~ 12.2 km ): 36+ apartments.
Petrcane ( ~ 14.6 km ): 201+ accommodation units.
Vrsi ( ~ 14.6 km ): 34+ apartments.
Beach Pedinka, Vir ~ 0,6 km from Tomić Gravel beach: ideal for families, with caffe bar. Excellent choice of watersports.
Beach Kozjak ~ 1,9 km from Tomić Gravel beach: ideal for families, with restaurant, with caffe bar, equipped with showers. Excellent choice of watersports.
Beach Jadro ~ 2,6 km from Tomić Gravel beach: recommended for young people, with restaurant, with caffe bar, equipped with showers. Excellent choice of watersports.
Beach Biskupljaca ~ 3 km from Tomić Concrete beach: ideal for families, with restaurant, with caffe bar, equipped with showers. Excellent choice of watersports.
Beach Mala Slatina ~ 3,1 km from Tomić Concrete beach: ideal for families, with restaurant, with caffe bar, equipped with showers. Excellent choice of watersports.


We offer excellent experiences with a broad exposure clinically and socially that will serve a physician for the remainder of their career. The academic setting is strong and the opportunity to work with and within a vulnerable community offers learning that will be critical for 21st century success.
What is the Family Medicine Clinic like?
Providers work with multidisciplinary teams with pharmacists, diabetic and chronic disease APP's and other to promote maximal healthcare outcomes for our patients.
NS is a rotation during your HO-2 year. You will be the senior resident running the B6 inpatient unit overnight on a shift from 6PM-6AM for a 2 week period. You will be working with an assigned intern and have 4 days off. Prior to being assigned NS, you will have succeeding in rotating through the MICU at HFH.
The unique patient population we serve.
Our FMC's are located in the city limits and we work with patients of varying socioeconomic situations. The pathologic burden is heavy, as is the psychosocial burden. This provides the learner a unique opportunity in system based care and practice based learning. Medical knowledge requirements are high with this population of patients. Both ambulatory and inpatient medical care brings daily challenges and surprises.
Our curriculum is heavily focused upon timely and appropriate communication between physician and patient. Our behavioral science curriculum is embedded at both IP and ambulatory sites, reinforced with didactics, resident projects, and community assignments.
Process and assists us in the development of this competency with our learners.
EBM is critical in the delivery of high quality care, but the communication of that evidence and sharing those decision making pathways is critical in the span of an appointment. We focus on giving our residents usable skills that reinforce the visit experience for both them and their patient. Residents experience a longitudinal curriculum, and a presentation in EBM prior to graduation assuring their own fluency in the principles and standards of this skill.
What fellowships are available at HFH?
We offer a Family Medicine Sports Medicine Fellowship at HFH and Sleep Medicine that accepts Family Medicine residents.


Responsible for statistical aspects of clinical and pre-clinical research projects including study design, sample size estimation, patient randomization, case report form development, writing statistical analysis plans, statistical analysis and interpretation of data, and reporting of results. Work with SAS programmers as part of a biostatistics team.
Knowledge of applied parametric and nonparametric statistics, strong SAS programming skills, and the ability to translate client needs into statistical practice and the ability to educate clients in the use of statistics are essential.
Candidate with 2+ years experience conducting statistical analysis of clinical research data.
Experience using SAS, Oracle Clinical, and/or ClinTrial.
Typically has a Masters or a Ph.D. degree in statistics, biostatistics, or related field with at least 6 years of pharmaceutical or related experience.


text:
Postwoman suffered 'large open' wounds in Doberman attack
She needed 45 stitches
Michael Steed
Lisa Porter
Sharon Singer's treatment continues (Image: David Singer)
The owner of a dangerous dog has been ordered to pay £1,000 compensation to a postwoman who suffered large open wounds to her arms and cuts to her hand and fingers after a savage attack.
Postwoman and mother of two Sharon Singer, 47, was attacked by the female black Doberman as she delivered mail to the home of Julian Palfreyman at Gold Fen Bank in Wrangle on the morning of July 4.
Mrs Singer, who had been a postwoman for 19 years, went to the detached house where she had been many times before, to deliver the morning mail.
Postwoman 'may never recover' from savage mauling by two dobermans
She let herself in through a gate to put a letter into the letter box when she heard barking and then saw a Doberman she had never seen before, barking at her.
Sharon Singer suffered horrific injuries (Image: Sharon Singer)
Mrs Singer was afraid the dog might attack her, she put her hands up in front of her and backed slowly away.
But without any warning, a second Doberman 'came from nowhere' and attacked her.
Julian Palfreyman was charged with having a dangerous dog and has appeared at Boston Magistrates' Court where he pleaded guilty to the offence.
Jim Clare, prosecuting, said Mrs Stringer was bitten severely on the left forearm and she screamed out in pain and for help, but was then bitten on the arms, legs and hands.
'A paper boy would not have survived dog attack that left my wife in hospital'
He said that in a 'few seconds' the owner, Mr Palfreyman, came into view and shouted at the dogs.
He grabbed the dog, called Mezzie, that was attacking Mrs Singer, but it broke free and again attacked her, biting her on the lower back and bottom, until she finally got out of the gate and into her van.
She was bleeding and in a lot of pain and Palfreyman then took her to the Accident and Emergency Department at Boston Pilgrim Hospital, where she spent four days.
Mr Clare said Mrs Singer had large open wounds to her left forearm, cuts to her hand and fingers and an open wound on her right arm.
He said she had a total of 19 wounds which needed 45 stitches and that five weeks later she still had difficulty sleeping, was still in pain and was suffering from muscle wasting.
She had hours of surgery to repair muscles, tendons, nerves and ligament damage and she may need another operation but was left with no feeling in the little finger of her left hand.
He said she was that day undergoing an operation in London to try and repair the damage to the nerves in her hand and there was no timescale for a return to work, as she had no feeling in her hand and was unable to drive.
Mr Clare asked the magistrates to order the immediate destruction of the dog which Palfreyman accepted was dangerously out of control although he told police it had never shown any aggression previously.
Sharon Singer shares her round with husband David (Image: David Singer)
In mitigation, Andrew Wheldon said Palfreyman did not minimise the seriousness of what had happened but the incident 'could not have been foreseen'.
He said he had only moved into the house the day before and it was securely fenced.
Palfreyman had let the dogs out while he made a cup of tea and ran outside when he heard the screaming.
Bereaved mum urges drivers to activate new feature on their iPhones
Mr Wheldon said the police had seized both dogs but had released back to Palfreyman the one that was not involved in the attack.
He said there was now increased security at the house with double gates, and anyone visiting had to ring a bell to obtain access through an electric gate that could only be opened from the inside.
The postbox was now outside the property, he told the magistrates and asked the not to order the immediate destruction of the dog.
He said Palfreyman had had dogs all his adult life and had owned Mezzie since it was a pup.
The incident had changed his life, he told the court, because of worry for Mrs Singer and the incident had triggered his split from his wife.
Ram savagely attacked by dog in a secured field
The magistrates told Palfreyman it was a 'very serious matter' and that Mrs Singer had suffered 'horrendous injuries' for which they gave their sympathies.
However, they said that because of the steps he had taken at his house, they would not order immediate destruction of the dog but would issue a contingent destruction order which effectively acts as a kind of 'suspended sentence' on the dog.
They ordered Palfreymen to carry out 200 hours of unpaid work for the community and to pay £1,000 compensation to Mrs Singer and court costs of £85.
Speaking before the case, Mrs Singer's husband David, who jobshared the round with his wife said: "Sharon is lucky to be alive. It's not just posties who are at risk of being attacked by dogs, it's also people who leaflet and deliver newspapers.
"A paperboy would not have lived after an attack like what happened to Sharon.
"We have both been bitten before but this was at the other end of the scale. You don't go to work to get injured.
"I just want to make dog owners more aware of their responsibilities. Nine times out of ten people are brilliant but there's a small percentage who are not.
"It's unbelievable the amount of times I've heard people say: 'He's never bitten anyone before.'
"OK, he's never bitten before, but look at what he can do when he does.
"We are campaigning for people who have dogs to install a metal post box outside the perimeter of their property.
"If you can afford to keep a dog, you can afford to spend £10 on a metal box."


Posterity (Express) opens doors again.
Posterity also hopes that customers will approach with their requirements for items we no longer 'held in stock'. 32 years experience and contacts in U.K. and Europe means they can source most things, especially for landscaping and garden ornament.
The gallery will aim to open in November, and will concentrate on art for investment. Paintings and limited edition prints by 20th.c artists of note, whose work is considered sure to appreciate in value year after year.
The web site will be kept bang up to date.
New address: Unit 4, Old Wharf Trading Estate, Ledbury, HR8 2PQ.
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Andy Cohen will never forget his best friend.
It's been more than 10 years since Natasha Richardson passed away after her fall on a Canadian ski slope.
But after nearly a decade, the Watch What Happens Live! host continues to live his life with one of his dear friend's greatest mantras.
While appearing on the latest Today Originals episode of "Quoted By … With Hoda," Andy recalled a quote that remains at the center of his life.
"One that I sat with was the chorus of 'Cabaret.' It's the title track from the musical Cabaret. 'What good is sitting alone in your room? Come hear the music play. Life is a cabaret, old chum. Come to the cabaret,'" Andy recalled to Hoda Kotb. "I think it's a mantra almost. Come hear the music. What are you doing inside? Live your life! Go out."
He continued, "And then the tenth anniversary of my dear friend Natasha Richardson's very untimely passing and if anything represented her, it was this sentiment. And not only did she win the Tony for this song, but there would be times where late at night, a group of friends would gather and we would coax her into singing that song."
Andy said the song and lyrics were "like a rallying cry" for how to live your life on this earth.
"It was so the way she lived her life every minute of it," he explained. "It speaks to me as something that remembers my friend and it speaks to me as part of the way I live my life in New York City and I know that my child will only add to that. I'll bring him out to hear the music play. It's how I want him to live my life."
During the candid discussion, the new dad also opened up about parenthood. More than two months after welcoming a baby boy via surrogate, the Bravo host believes baby Benjamin arrived right on time.
"I feel like everything that has happened in my life has happened at the right time and for a reason," Andy shared. "I feel like he came at the right time. I wasn't ready for him to come. I was too busy at the cabaret. Now I'm ready to bring him to the cabaret."


text:
IPC re-approves Rix's Creek mine extension
Industry News / Mining & Resources
Bloomfield's Rix's Creek South Project is approved and will allow mining operations to continue for 21 years
It is estimated to produce an additional 25 million tonnes of product coal with royalties of more than $104 million
The Rehabilitation Strategy and Rehabilitation Management Play are required to be updated every 3 years
WOW, the Independent Planning Commission (IPC) is adopting shift work hours like the mines it approves.
On Saturday, when everyone was looking at Bureau of Meteorology radar to check out some very welcome rainfall, the IPC released a statement saying – a week after approving the Rix's Creek mine extension and then almost immediately saying that approval was invalid – it is now approved.
So for the record Bloomfield's Rix's Creek South Project is approved and will allow mining operations to continue for a further 21 years.
Bloomfield Collieries Pty Ltd lodged a state significant development (SSD) application to expand its Rix's Creek South Mine, 5km northwest of Singleton, and continue open cut mining operations there until 2040.
Under the proposal, it is estimated an additional 25 million tonnes of product coal, with royalties of more than $104 million, would be recovered from the mine over that period.
This decision will please the mine's owners, employees and contractors, the member for Upper Hunter Michael Johnsen, Federal member for Hunter Joel Fitzgibbon and the NSW Minerals Council.
For those opposed to further extensions of mining operations in the Singleton Local Government Area the passing comments by the IPC on air quality based on 'best practice' outcomes raises many concerns given the significant number of air quality alerts for the surrounding area.
Commissioners Mary O'Kane (Chair), Andrew Hutton and Tony Pearson were appointed to consider the application say in their statement:
After carefully considering all the evidence and weighing the community's views, the Commission has today (Saturday 12 October 2019) determined to approve with conditions the SSD application, which will allow an expansion of mining activities to the south of the existing Pit 1 and northwest of the existing Pit 3.
In its Statement of Reasons for Decision, the Commission concluded the application is in the public interest – because (in summary):
it is "in respect of an existing brownfield site, with existing operational mining infrastructure"
"noise and vibration impacts have been adequately assessed" and "appropriate mitigation, management measures and accelerated noise attenuation are proposed"
"air quality has been adequately assessed and found by the Department to be aligned with contemporary best practice for NSW coal mines, and improvements should continue to be pursued over time" and "the Ministry of Health was satisfied with the assessment and recommended conditions"
"GHG emissions have been adequately minimised as far as practicable and within the capability of the Applicant's control… Conditions of consent have been included to require the Applicant to take all reasonable steps to improve energy efficiency and to reduce the Application's GHG emissions over the life of the Application"
"biodiversity offsets have been quantified and a staged offset strategy identified to retire the necessary biodiversity offsets for the Application"
"biodiversity impacts have been appropriately assessed and can be managed through conditions of consent"
"water resources have been appropriately addressed and conditions of consent proposed to ensure impacts are appropriately managed and mitigated"
"rehabilitation and risk of early closure has been appropriately assessed… The requirement for a Rehabilitation Strategy and Rehabilitation Management Plan, updated every 3 years, and incorporating risks of unplanned closure into risk assessments, provides assurance that the Applicant's closure arrangements will consider unplanned closure and have regard for the Council's strategic planning and community expectations given the close proximity of the Application to the Singleton Township and Camberwell"
"visual impacts are appropriately assessed and considered to be relatively minor overall given the existing operation"
"heritage impacts have been assessed and would be appropriately managed through conditions of consent"
"blasting impacts have been adequately assessed and would be appropriately managed"
"traffic impacts have been adequately assessed and would be appropriately managed"
"social and economic impacts have been adequately assessed appropriately managed, consistent with the guidelines for the economic assessment of mining and coal seam gas proposals… The Application would generate economic and social benefits as a result of employment opportunities and revenue to the State"
"A planning agreement would be entered into by the Applicant and [Singleton] Council which would provide further benefits locally"
"the Applicant has adequately addressed the recommendations in the Commission's Review Report" and
"the Application meets the principles of [Ecologically Sustainable Development]".
The Commission has imposed conditions on its approval which are designed to "prevent, minimise and/or offset adverse social and environmental impacts; set standards and performance measures for acceptable environmental performance; require regular monitoring and reporting; and provide for the ongoing environmental management of the development."
Originally published by Muswellbrook Chronicle.
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Just in time for Giving Tuesday, the Web site — whose tag line is "Shop for Good" — launches.
Pamela Trefler had an "a-ha" moment after she realized that the castoffs in her wardrobe were worth serious money.
After a closet clean-out session, a consignment store wrote her a check for $40,000, which she promptly used to buy a computer lab for a Boston school. Trefler knew other women had unwanted items in their closets that could be re-sold to benefit nonprofit groups. From that seed, Union & Fifth was born. Just in time for Giving Tuesday, the Web site — whose tag line is "Shop for Good" — launches today.


text:
Kellee Edmonds
edmondsk@aascu.org
AASCU NAMES 36 HIGHER EDUCATION LEADERS TO ITS 2022 EMERGING LEADERS PROGRAM
WASHINGTON, June 14, 2022 – A new cohort of 36 higher education professionals will participate in the 2022 Emerging Leaders Program (ELP), a premier leadership development program of the American Association of State Colleges and Universities (AASCU). The program was created in 2015 to prepare early to mid-career academic and administrative emerging leaders who aspire to advance to administrative roles with greater responsibility and oversight.
"The diverse and motivated campus leaders in the ELP class of 2022 reflect the strength and value of what happens when the AASCU network comes together," said Dr. Charles L. Welch, president of the Arkansas State University System, former chair of the AASCU Board of Directors, and ELP coordinator. "We are pleased to bring the ELP cohort to Washington, D.C., for three days of in-person programming for the first time in three years and look forward to lively discussions and insightful learning experiences that will inspire and equip participants to shape our institutions to promote inclusivity, equity, and student success. I look forward to a great year of learning and personal development."
With a curriculum designed to develop the skills and competencies necessary for the next generation of higher education leaders, the 2022 ELP cohort will engage with a rich and experienced bench of faculty to cover topics such as equity and social justice leadership, navigating and leading through change, finance and administration, leading as a provost, and more. The group will meet for an in-person retreat, monthly virtual workshops, and small-group sessions for reflection and study of promising practices. Additionally, each member of the ELP cohort will demonstrate their leadership skills to their campus community by leading an on-campus project or initiative with the support of their president/chancellor or cabinet-level administrator.
View the full cohort list.
The American Association of State Colleges and Universities (AASCU) is a Washington, D.C.-based higher education association of nearly 400 public colleges, universities, and systems whose members share a learning- and teaching-centered culture, a historic commitment to underserved student populations, and a dedication to research and creativity that advances their regions' economic progress and cultural development. These are institutions Delivering America's Promise.


text:
layout: post
title: "Dilema Blogger Pencari Dollar"
subtitle: "Menjadi dilema di antara mendapat ranking penelusuran dan dollar."
description: "Rencana Google menyingkirkan iklan interstitial menjadi dilema bagi Blogger yang ingin mendapatkan dollar dan juga ranking di mesin penelusuran."
date: 2016-08-24
author: "Adhy Suryadi"
tags: [ Blogging ]
header-img: "img/post/dollars-online.jpg"
image: "img/post/dollars-online.jpg"
social-js: "https://cdn.ampproject.org/v0/amp-social-share-0.1.js"
iframe-js: "https://cdn.ampproject.org/v0/amp-iframe-0.1.js"
accordion-js: "https://cdn.ampproject.org/v0/amp-accordion-0.1.js"
---

Baru-baru ini Google telah mengumumkan bahwa mereka akan meningkatkan hasil penelusuran mobile yang benar-benar memberikan pengguna mobile mendapatkan pengalaman pencarian Google di perangkat mereka menjadi lebih baik.

Seperti telah kita ketahui bahwa dua tahun yang lalu Google telah memberikan label "Mobile-friendly" di hasil pencarian untuk website yang benar-benar mendukung tampilan mobile sehingga pengguna tidak perlu melakukan zoom pada konten yang ingin dibacanya.

Dengan begitu pengguna mobile langsung mengetahui bahwa website yang akan dibacanya sudah mendukung tampilan mobile. Namun kini Google akan menghapus label "mobile-friendly" untuk merapihkan hasil pencarian, namun "mobile-friendly" sebuah website akan tetap menjadi faktor ranking penelusuran.

Namun Google juga telah menemukan banyak website yang menggunakan hal yang cukup mengganggu pengguna mobile. Banyak website menggunakan iklan pop-up yang tampil sebelum pengguna membaca konten rujukan hasil pencarian, bahkan iklan pop-up ini berkesan mengelabui pengunjung website.

Untuk itu Google pada 10 Januari 2017 mendatang akan benar-benar menyingkirkan website dengan iklan pop-up seperti itu dari hasil pencarian, sehingga pengguna mobile tidak merasa dikelabui oleh mesin pencari karena menampilkan hasil pencarian dengan link yang seolah menipu.
{% include adsense_middle_post.html %}
Tentunya hal ini menjadi dilema bagi Blogger pencari dollar di internet. Di satu sisi ingin mendapatkan penghasilan dengan memasang iklan. namun di sisi lain iklan yang dipasang malah berbenturan dengan aturan mesin pencari Google.

Seperti kita ketahui bahwa iklan pop-up merupakan sebuah unit iklan yang paling mudah mendapatkan dollar, untuk itu banyak Blogger yang memasang iklan pop-up ini.

Namun tentunya hal ini (iklan pop-up) menjadi tidak berguna jika web/blog tidak muncul di hasil pencarian yang notabene hasil pencarian merupakan pintu masuk pengguna terbesar ke sebuah web/blog. Jika tidak ada pengunjung yang masuk maka tidak akan ada penghasilan yang didapat.

Namun Google juga memberikan penjelasan lainnya. Jika web/blog memiliki konten yang kuat dan benar-benar bagus dan relevan dengan kueri penelusuran, maka web/blog masih memiliki kemungkinan memiliki ranking untuk tampil di halaman hasil penelusuran walaupun menggunakan iklan pop-up.

Jadi sampi saat ini Google masih memegang teguh prinsip "Content is the King". Jika blog Anda ingin bertahan atau memiliki kesempatan di halaman pertama Google walaupun menggunakan iklan pop-up, maka silahkan buat konten blog yang benar-benar bagus dan original yang memenuhi kriteria mesin pencari.

**[Sumber bacaan](https://webmasters.googleblog.com/2016/08/helping-users-easily-access-content-on.html "Sumber bacaan"){: target="_blank" rel="nofollow"}**

redpajama_set_name: RedPajamaGithub


Below are our 2018-2019 tuition rates. Tuition increases about 5-7% annually.
Epiphany Early Learning provides breakfast, morning snack, lunch and afternoon snack. All meals are included in tuition prices.
Click here to see a sample menu. Our chef, Julia, makes meals with 95% organic and locally sourced food.
Epiphany Early Learning values a diverse community and our goal is to have a moderate scholarship fund so we can provide financial aid to families. Currently we are able to offer minimal scholarships. If you are interested in applying for a scholarship, please contact the director.


text:
Q: how to display the selected check box value in javascript I want to dispaly the selected check box value if i click on the print button
here is my code
<! DOCTYPE html>

<form>
    <input type="checkbox" id="bk" name="vehicle" value="Bike">I have a bike

    <input type="checkbox" id="cr" name="vehicle" value="Car">I have a car

     <input type="submit" value="   Print   " size="30" onClick="checkbox()">
</form>

If I select Bike check box and click on print button, it should display the only Bike.

A: i have edited your code and modified according to your needs. please go through the code.
    <! DOCTYPE html>

<form>
    <input type="checkbox" id="bk" name="vehicle" value="Bike">I have a bike

    <input type="checkbox" id="cr" name="vehicle" value="Car">I have a car

     <input type="submit" value="   Print   " size="30" onClick="return checkbox();">
</form>

A: This function would create an array with the values of each checked item inside your form:
function checkbox()
{
  var checked = [].reduce.call(document.forms[0].vehicle, function(current, item) {
    if (item.checked) {
      current.push(item.value);
    }
    return current;
  }, []);
  alert(checked);
}

Demo
Here, document.forms[0].vehicle returns both checkboxes with the name vehicle inside your form; I'm using document.forms[0] because you didn't give the form a name :)

A: heres ur code.. this should solve ur problem..
  <! DOCTYPE html>

<form>
    <input type="checkbox" id="bk" name="vehicle" value="Bike">I have a bike

    <input type="checkbox" id="cr" name="vehicle" value="Car">I have a car

     <input type="submit" value="   Print   " size="30" onClick="return checkbox();">
</form>

redpajama_set_name: RedPajamaStackExchange


text:
Q: C*-Algebras and Operator Theory - Gerald J. Murphy Theorem 4.1.2 I'm stuck On the theoremTheorem 4.1.2, from   C*-Algebras and Operator Theory by Gerald J. Murphy

4.1.2. Theorem. Suppose that $(p_\lambda)_{\lambda\in\Lambda}$ is a net of projections on a Hilbert space $H$.
(1) If $(p_\lambda)$ is increasing, then it is strongly convergent to the projection of $H$ onto the closed vector subspace $(\cup_{\lambda}p_\lambda(H))^-$.
(2) If $(p_\lambda)$ is decreasing, then it is strongly convergent to the projection of $H$ onto $\cap_\lambda p_\lambda(H)$.

I have no idea how to deal with. Any hints or suggestions
would be welcome!

A: As established earlier in Murphy, an increasing net of projections that is bounded above by a self-adjoint element converges strongly to its supremum. Hence, for $(1)$ we must check that if $p$ is the projection onto $K= \overline{\bigcup_\lambda p_\lambda H}$, then $p$ is the supremum of the net $\{p_\lambda\}$.
This is rather easy, since $p \geq p_\lambda$ for all $\lambda$ because $pH \supseteq p_\lambda H$ by definition of $p$. Thus $p$ is an upper bound for this net.
Moreover, if $q \geq p_\lambda$ for all $\lambda$, then clearly $q \geq p$ and
$$(p-q)p_\lambda = pp_\lambda-q p_\lambda = p_\lambda- p_\lambda = 0$$
so that $p-q$ annihilates the subspace $K$. Hence,
$$0 = (p-q)p = p-qp= p-q$$
and thus $q=p$. Thus, $p$ is the least upperbound. Can you now do $(2)$ yourself?


You will find the Fusion Forms Tools on their own toolbar. If you do not see the tool bar you can display it by selecting the menu item View then putting a check in front of the Form Tools list item.
You can select the Form Area tool from the Form Toolbar and drawing a rectangle to hold your form.
The Create Form dialog box will open to ask you what type of form you want.
You can draw your own layout region, text box, or table using those tools, then put a check in the box on the selected object properties palette to set that object as a form. There is also an option to select the entire layout as the form on the layout properties palette. This is basically the same as using the Form Area tool but it is a manual process. This second method is good if you already have an area on your page that you want to make a form.
What ever method you choose to hold your form is fine. Use the tools from the Form Toolbar to select and place the form elements you want to use. Just select the form element from the toolbar and draw a rectangle where you want that form element to be shown. The Form Toolbar has the following form elements you can use.
Multi-Line: This is a multiple line input box, as the input grows a scroll bar appears.
Field Label: Select this tool to place text you wish to be associated with a form field. The label will be part of the form processing and next to the form contents in the email that is sent. In the case of radio buttons you can select several buttons to associate with one label. Select the Form Label Inspector tab , then select the form element from the drop-down box to associate with the form label.


As Seattle trademark and copyright lawyers, we help clients protect their intellectual property through registration. We also help clients desiring to license trademarks and copyrights through licensing.
For clients seeking trademark and service mark registration, we assist in first determining whether there are any potentially conflicting trademarks or service marks. We additionally prepare the registration filings, and, when we know how the mark is to be used, we determine the appropriate class(es) for which registration should be made.
For clients doing business internationally, it's important to determine whether registration should be made in countries outside the United States. If such registrations are desired, we can work with local counsel in other countries to pursue appropriate registration.
In connection with registration, we also advise clients concerning the initial registration fees required, the term of the initial registration, and the dates at which renewals should be undertaken in order to preserve protection.
After the registration documents have been filed, we work on behalf of clients to answer any questions that may be forthcoming from the United States Patent and Trademark Office, including any objections that they may make to registration.
We prepare and file copyright registrations on behalf of clients in order to better protect their works. Additionally, we counsel companies of matters concerning copyright law, including expiration of copyright and licensing.
We also assist companies who may become aware of infringing uses of trademarks, service marks, or copyright.


Dale Haralson was the kind of patient some doctors would rather avoid. In 2005, the Tucson, Ariz., lawyer had a triple-bypass operation, then contracted an infection that spread to his chest, lungs, blood and bone. He needed more surgery and was told he would need a permanent feeding tube. But few surgeons wanted to take his complex case.
A former partner recommended that he see Steven D. Knope, a local primary-care doctor who treats a small group of patients in exchange for an annual fee. Dr. Knope took over Mr. Haralson's care, and found a skilled and willing surgeon and a pulmonologist who said that, instead of a feeding tube, he just needed to use an inhaler every day.
Today, Dr. Knope handles all the routine medical care for Mr. Haralson and his wife, Betty, and tracks the couple's general health and fitness. Together, they pay him $10,000 a year, and think it's money well spent. "All you need is one crisis and a good outcome and you know it's worthwhile," says Mr. Haralson, age 71, who exercises regularly, still practices law and still eats chocolate cake.
In particular, there are a few points worth highlighting.
1) Concierge doctors take care of some complex, challenging patients whose needs could not be met in the standard fast-food medical model. If a doctor taking Medicare or conventional insurance has to average 7 minutes per office visit just to pay the overhead, there's no way he can do justice to his patient's medical needs.
2) Concierge medicine is not just for the wealthy. Those services are affordable to patients of modest means, if they are willing to budget for them and place a high priority on good medical care. It's no different from the fact that some parents who really value their kids' education will make it a priority to budget carefully and send their kids to a good private school rather than subject them to the harms of a government-run public school.
3) Both parties win because concierge medicine represents a shift towards free-market principles. Patients receive the care they need at a fair price. Physicians are able to practice according to their medical conscience, while being appropriately compensated. This is just an example of the broader virtue of free markets, where buyers and sellers exchange money for services on a purely voluntary basis. In a free market, the exchange occurs only if both parties deem it to be in their benefit. The concierge physician succeeds only by offering a genuine value to his patient. Hence, they are willing and eager to do so, as the many happy patients in the article can attest to.
Maintain your private medical care if at all possible. If you are relatively healthy, look into a high-deductible health insurance plan linked to a Health Savings Account (HSA). Start putting money away in that HSA for a rainy day. Find a "concierge physician" or doctor with whom you can establish a direct financial relationship; someone who will act as your medical advocate in a system that is broken and will only get worse. You get what you pay for and medicine today is no different.
After all, it's your life at stake.


text:
Blast From the Past: Step Inside 'The House of Seven Gables'
History haunts a bewitched Brahmin family in Massachusetts. Relationships between its members and others, though, prove more meaningful than a cure for the curse.
A romance written by Nathaniel Hawthorne immediately after the 'Scarlet Letter' (q.v.) and published in 1852. The scene is laid in Salem, the author's native town, which he had quitted for a residence in western Massachusetts when his fellow townsmen became incensed over the personalities in the Introduction to the 'Scarlet Letter.'
Something of the resentment shown in this unfortunate sketch is seen in the creation of Judge Pyncheon, the villain of 'The House of the Seven Gables,' who is said to have been drawn after the local politician whom Hawthorne held chiefly responsible for his removal from the Salem custom-house. Other materials in the tale which have a personal or family origin are the inherited curse of the Pyncheons, which was really invoked on an early Hawthorne who was one of the witch-judges, and the account of lost title-deeds to vast estates in Maine, which was one of the traditions in the family of the author's mother.
The story involves the aristocratic Pyncheons, who own the house of the seven gables, and their humble fellow-townsmen, the Maules. Between these two families has existed a strange relationship since early colonial times when Matthew Maule pronounced a dying curse on his enemy, a Pyncheon who had brought about his condemnation as a wizard. The reader, however, cares far less for the plot which brings about the removal of the curse than for the treatment of characters and scenes, and the atmosphere which pervades the whole.
There is an element of humor which is lacking in the 'Scarlet Letter' and no work of the author shows better his delicacy and subtlety. The portrayal of Hepzibah, the poverty-stricken but proud descendant of the Pyncheons, is masterly in its blending of sympathy and playfulness. While inferior to the 'Scarlet Letter' as a unified work, 'The House of the Seven Gables' is richer in passages that linger in the reader's memory, and is the romance of Hawthorne most frequently studied in schools and recommended for the reading of young persons.
Encyclopedia Americana, 1920


After going down early, the Lady Crushers battled back all night long.
Bishop McCort never led in the game until the 61st minute when Alexis Regula sent a crossing pass towards the goal and Lady Rams keeper Kylie Orndorf misplayed the ball. It slipped through her fingertips and into the goal. Orndorf was solid for the Lady Rams stopping five of the Lady Crushers' eight shots in the first half and finished with seven in the game.
Bishop McCort held a significant advantage in time of possession after going down 2-0. It was in Penns Valley's half of the field for 30 of the 40 first minutes. While holding that advantage, the Lady Crushers outshot the Lady Rams 12-4. They also amassed eight corner kicks to Penns Valley's none.
The Lady Rams (8-7-4) felt the pressure of not being able to get anything going offensively. When they got their chances, they seemed to rush their shots and misfired on the net.
Right before Regula's eventual game-winning goal, Penns Valley had an opportunity to claim the lead on a free kick — its first shot on goal since five minutes into the game. Cheyenne Swartz booted a kick that ricocheted of the crossbar. Keira Whitman corralled the rebound but her shot went wide right.
The Lady Rams sprinted out to a two-goal advantage five minutes into the game thanks in part to sloppy passing from the Lady Crushers.
Destiny Andrus rocketed a Bishop McCort turnover in the box to the bottom left corner of the net and past Crushers keeper Maura Clark 2:30 into the game. Two minutes later, Andrus connected with Valerie Kubalak, and Kubalak sent it to the same corner as Andrus to make it 2-0.
Bishop McCort rallied to tie the game thanks in part to Katie Downey and Regula.
Regula put the Lady Crushers on the board at the 28th minute mark when she took a pass from Haley Thomas on a free kick and caught Penns Valley sleeping. Four minutes later, Downey fired one from just outside the box and past Orndorf.
Bishop McCort will next play No. 1 seed Bishop Guilfoyle in the semifinals on Wednesday at a site and time to be determined.


text:
Q: Passing a method as an argument I have a method SaveChanges<T>(T object) that is frequently called throughout my code, except depending on the action calling the method, there would be a different method called from within SaveChanges.  Something like this...
protected void SaveChanges<T>(T mlaObject, SomeFunction(arg))
    where T : WebObject
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        SomeFunction(arg);
    }
}

Usage Examples:
SaveChanges<MlaArticle>(article, article.Authors.Remove(person)) //person is an object of type MlaPerson
//OR
SaveChanges<MlaArticle>(article, article.RelatedTags.Remove(tag)) //tag is an object of type Tag
//OR
SaveChanges<MlaArticle>(article, article.RelatedWebObjects.Remove(location)) //location is an object of type MlaLocation

I've read up on delegate methods but I'm a little confused as to how to implement this with my requirements or if my requirements warrant use for delegates at all.
EDIT: Also, would it be possible to pass multiple Actions?

A: UPDATE I was a little unclear from your question if the arg passed in is being used anywhere else in the method, it doesn't look like it is, so you can just take an Action and use a lambda to specify the delegate to call with the captured argument:
protected void SaveChanges<T, TArg>(T mlaObject, TArg arg, Action undoFunction)
    where T : WebObject
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        undoFunction();
    }
}

To which you can pass:
SaveChanges(article, () => article.Authors.Remove(person));

Or if it's the myObj itself, in which case (as sixlettervariables already answered) you can just pass it back in a delegate as per his code.
Or, whether the arg is different from mlaObject and you want to also do other things on it in the code, in which case you could do:
protected void SaveChanges<T, TArg>(T mlaObject, TArg arg, Action undoFunction)
    where T : WebObject
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        undoFunction(arg);
    }
}

And then have:
SaveChanges(article, person, article.Authors.Remove);

A: protected void SaveChanges<T,U>(T mlaObject, Action<U> action, U arg)
    where T : WebObject
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        action(arg);
    }
}

Hope I understood the question correctly...

A: Your SaveChanges method would look something like:
protected void SaveChanges<T,TArg>(T mlaObject, TArg arg, Action<T,TArg> someFunction)
    where T : WebObject
{
   ...
}

Called like:
SaveChanges<MlaArticle,Person>(article,person, (article,person) =>  article.Authors.Remove(person))

A: How about:
protected void SaveChanges<T>(T mlaObject, Action<T> rollback)
    where T : WebObject
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        rollback(mlaObject);
    }
}

Called like:
this.SaveChanges(myObj, x => article.Authors.Remove(x));

Now, from a second read of your question, I don't see a point in passing the mlaObject as it is never used.
// this.SaveChanges(
//     () => article.Authors.Remove(author),
//     () => article.RelatedTags.Remove(tag));
protected void SaveChanges(params Action[] rollbacks)
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        foreach (var rollback in rollbacks) rollback();
    }
}

// Overload to support rollback with an argument
// this.SaveChanges(
//     author,
//     article.Authors.Remove,
//     authorCache.Remove);
protected void SaveChanges<T>(T arg, params Action<T>[] rollbacks)
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        foreach (var rollback in rollbacks) rollback(arg);
    }
}

A: protected void SaveChanges<T>(T mlaObject, Action<T> functionToCall)
{
    try { this._db.SaveChanges(); }
    catch (Exception e)
    {
        Console.WriteLine("Error: " + e);
        functionToCall(mlaObject);
    }
}

Call like this:
SaveChanges(actualArticle, article => article.Authors.Remove(person));

I left out the WebObject bit as it was not used in the function at all.

A:
if my requirements warrant use for delegates at all.

If you want the SaveChanges method to perform some function you have two options

*

*have it perform the function directly (code inside the method, or call some second method from inside the method); or

*provide the function to the SaveChanges method as a delegate.

When to use each of these is a design choice that is up to you and will depend on the scenario, the overall solution and your preference.
Benefits of the first

*

*Able to see every possible outcome of the SaveChanges method in one place

*Less confusing for someone who doesn't know how delegates work

Benefits of the second

*

*Able to exclude every possible function from the SaveChanges method (it doesn't require an enormous case or if else if else if)

*The functions that are passed to the SaveChanges method can be above it in the call stack, it doesn't need to know what they are or how they work, they can do things it doesn't understand, and they can be reused - called elsewhere or used as delegates in other functions.

I think the first point is the main one here. If you are only handling a couple of scenarios then it's okay to have a an if else if else if, but if you get more than a handful of options and prefer a more generic SaveChanges method then psdd that delegate.


text:
This article was published on January 12, 2016
Google researcher left speechless as Trend Micro exposes all customers to attack
Former TNW employee
Owen was a reporter for TNW based in Amsterdam, now a full-time freelance writer and consultant helping technology companies make their word (show all) Owen was a reporter for TNW based in Amsterdam, now a full-time freelance writer and consultant helping technology companies make their words friendlier. In his spare time he codes, writes newsletters and cycles around the city.
This one's a head scratcher, but a thread on Google Code has revealed that Trend Micro exposed its antivirus customers to attack, after it installed a wide-open Node.js server by default on its customers' computers.
The Node.js server is part of Trend Micro's 'Password Manager' utility and installed with the company's antivirus software, as well as being set to open at startup, by default.
It leaves a number of ports open to the world, which could expose users to any website executing a malicious application on their machine by sending a JavaScript request. That means an attacker could easily remotely download code and execute it on a victim's machine without their knowledge.
If you thought it couldn't get any worse, it does.
Trend Micro also adds a self-signed security certificate to the user's certificate store, so the user won't see any HTTPS errors.
Tavis Ormandy, a researcher at Google's Project Zero vulnerability team, wrote in the thread that "this thing is ridiculous."
As research into the flaw continued, Ormandy found that an attacker could silently steal the passwords stored in Trend Micro's safe and decrypt them, all using the original flaw, telling the company that "I really hope the gravity of this is clear to you, because I'm astonished about this."
He then recommends that the company disable the feature, to protect users:
In my opinion, you should temporarily disable this feature for users and apologise for the temporary disruption, then hire an external consultancy to audit the code. In my experience dealing with security vendors, users are quite forgiving of mistakes if vendors act quickly to protect them once informed of a problem, I think the worst thing you can do is leave users exposed while you clean this thing up. The choice is yours, of course.
Ultimately, Trend Micro chose not to disable the tool, instead issuing an emergency fix to disable the first vulnerability two days ago.
The issue was first disclosed to Trend Micro on January 5, but wasn't resolved until today — even now it's only partially fixed, with the company issuing an emergency patch.
In response to Trend Micro dragging its heels, Ormandy said that he "[doesn't] even know what to say – how could you enable this thing *by default* on all your customer machines without getting an audit from a competent security consultant?"
The thread is now public and it's an incredible insight into how enterprise-grade antivirus software, which is supposed to protect users against threats, actually left them wide open to attack.
Published January 12, 2016 - 12:15 am UTC


text:
Netball Delay Marks Top-Level NZ Sport Shutdown
March 23, 2020 by Gary Hicks
The coronavirus COVID-19 pandemic has seen the top-flight sports scene in New Zealand laid bare after the Domestic Netball Championship got postponed for two weeks. This was the only professional competition still in place in the country but the halt to play has now been announced, with the earliest possible return target date set for April.
The games' opening rounds were completed and the second and third ones will be put back into place after the new governmental edict banning mass indoor gatherings of 100+ people gets rescinded.
Now every single top-level sport in New Zealand has had its events cancelled outright or postponed, along with almost all community sports.
All Six Nations games have been postponed and Super Rugby events have also all been called off. There is no indication as to when they'll return. But NZ Rugby is hoping to create a competition limited to domestic teams only for its five Super Rugby squads to fill the void left by the main competition being suspended indefinitely.
In related news, the NZ Warriors and Wellington Phoenix are currently basing themselves in Australia to stay in the NRL and A-League contests, respectively. The NZ National Basketball League has made it compulsory for all players and members of team management to self-isolate for two weeks after arriving from overseas, and the final two One Day Internationals that the Black Caps had scheduled in Australia were cancelled, along with the Australian Twenty20 Series set to unfold here later this month.
Silver Ferns coach Noeline Taurua says her side played an extremely "timid" game against England's national side – and that their performance marks a solid step backwards from the steely ….


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DOCTOR, satirist, actor, author, lecturer, writer, director - Jonathan Miller has packed an extraordinary amount into the past 45-plus years.
He qualified as a doctor of medicine in 1959 but changed course for the theatre when he was invited to join Peter Cook, Dudley Moore and Alan Bennett in a new satirical revue called Beyond The Fringe. It was a sensation which made Miller a household name.
But it was also the start of a career that would be lead him on a variety of intellectual and artistic adventures.
Tonight it is in his capacity as one of Britain's finest opera directors that he brings the New London Consort's remarkable semi-staged production of Monteverdi's masterpiece L'Orfeo to Lighthouse in Poole.
But he points out that although he has been an acclaimed director of operas internationally for well over 30 years, he didn't actually have any experience of the operatic stage before being asked to direct his first production.
"I had never even been to an opera before I was asked to do one," he told me. In fact, he says almost everything he's ever done has been as the result of a request.
"I never sought anything really. I only left medicine when I was invited to do Beyond The Fringe. Then I was asked to do a play at the Royal Court and then to produce Monitor for the BBC. One thing led to another. I've never gone looking for things. It's all been a sequence of invitations."
The production was originally commissioned by the South Bank Centre in 2003 so, needless to say, Miller has moved on to other projects. In fact he's currently working on an exhibition about the history of camouflage.
He tells me that camouflage was a term first coined during the First World War when aerial reconnaissance started making it easier to track the movement of troops.
Also, the bright red military tunic was becoming something of an embarrassment.
"Once you had a people's army as you did in the First World War You couldn't simply chuck lots of brightly coloured men into warfare and not care about the casualties."
Blimey it could almost make an opera!


Johnny Mercer´s much-deserved place in the songwriting pantheon of great creative artists is based on his superlative achievements as a major lyricist, a composer, a distinctive pop singer, and a recording executive.
Born in Savannah, Georgia in 1909, young John H. Mercer set off to New York during the Depression to become an actor on Broadway. At his first audition, he was told that what they needed was "girls and songs." He obliged by writing his first song, "Out of Breath and Scared to Death of You" that found its way into the Garrick Gaieties of 1930. He also met and married a chorus girl from that show, launching both a successful songwriting career and a 40+ year marriage. In time, he was also singing on radio with Benny Goodman and Paul Whiteman and collaborating with some of the major composers of Tin Pan Alley and Hollywood.
Among his hits beginning in the Thirties: "Lazy Bones" and "Skylark"(with Hoagy Carmichael); "I´m An Old Cowhand" (words & music by Mercer); "Too Marvelous for Words" and "Hooray for Hollywood(with Richard Whiting); "Jeepers Creepers" and "You Must Have Been A Beautiful Baby"(with Harry Warren); "Blues in the Night," "That Old Black Magic," "My Shining Hour," "One For My Baby," "Come Rain or Come Shine," "Ac-cent-tchu-ate The Positive"(with Harold Arlen); "Dearly Beloved," "I´m Old Fashioned"(with Jerome Kern); a string of Hollywood hits such as "On The Atchison, Topeka and the Santa Fe," "Laura," "In the Cool, Cool, Cool of the Evening"(1951 Academy Award winner), "Something´s Gotta Give," and as late as the Sixties with Henry Mancini, Academy Award winners "Days of Wine and Roses," and "Moon River."
Other Mercer standards: "Day In-Day Out," "And The Angels Sing," "Fools Rush In." "I Remember You," "Dream," and dozens of others forever linked to the Forties and Fifties.
Always a recognizable, jovial personality as a singer and songwriter, Mercer also founded Capitol Records and became a major commercial power in the music industry. Above all else, Mercer´s lyrics are his calling card: the trains, the rivers, the birds, the sights and sounds of the American landscape all repeatedly show up in a way that have made him America´s greatest songwriting Folk Poet.