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Q: How to check the value given is a positive or negative integer? Lets say i have the value 10 assigned to a variable;
var values = 10;

and i want to run a specific function if the value is a positive
if(values = +integer){
    //do something with positive
} else {
    //do something with negative values
}

How would this be achieved?

A: simply write:
if(values > 0){
//positive
}
else{
//negative
}

A: if(values >= 0) {
 // as zero is more likely positive than negative
} else {

}

A: if ( values > 0 ) {
    // Yeah, it's positive
}

A: if ( values > 0 ) {
    //you got a positive value
}else{
    //you got a negative or zero value
}

A: To check a number is positive, negative or negative zero. Check its sign using
Math.sign() method it will provide you -1,-0,0 and 1 on the basis of positive negative and negative zero or zero numbers
 Math.sign(-3) // -1
 Math.sign(3) // 1
 Math.sign(-0) // -0
 Math.sign(0) // 0

A: I know it's been some time, but there is a more elegant solution. From the mozilla
 docs:
Math.sign(parseInt(-3))

It will give you -1 for negative, 0 for zero and 1 for positive.

A: You can use the shifting bit operator, but it won't get the difference between -0 and +0 like Math.sign(x) does:
let num = -45;
if(num >> 31 === -1)
    alert('Negative number');
else
    alert('Positive number');

https://jsfiddle.net/obxptgze/

A: Am I the only one who read this and realized that none of the answers addressed the "integer" part of the question?
The problem
var myInteger = 6;
var myFloat = 6.2;
if( myInteger > 0 )
    // Cool, we correctly identified this as a positive integer
if( myFloat > 0 )
    // Oh, no! That's not an integer!

The solution
To guarantee that you're dealing with an integer, you want to cast your value to an integer then compare it with itself.
if( parseInt( myInteger ) == myInteger && myInteger > 0 )
    // myInteger is an integer AND it's positive
if( parseInt( myFloat ) == myFloat && myFloat > 0 )
    // myFloat is NOT an integer, so parseInt(myFloat) != myFloat

Some neat optimizations
As a bonus, there are some shortcuts for converting from a float to an integer in JavaScript. In JavaScript, all bitwise operators (|, ^, &, etc) will cast your number to an integer before operating. I assume this is because 99% of developers don't know the IEEE floating point standard and would get horribly confused when "200 | 2" evaluated to 400(ish). These shortcuts tend to run faster than Math.floor or parseInt, and they take up fewer bytes if you're trying to eke out the smallest possible code:
if( myInteger | 0 == myInteger && myInteger > 0 )
    // Woot!
if( myFloat | 0 == myFloat && myFloat > 0 )
    // Woot, again!

But wait, there's more!
These bitwise operators are working on 32-bit signed integers. This means the highest bit is the sign bit. By forcing the sign bit to zero your number will remain unchanged only if it was positive. You can use this to check for positiveness AND integerness in a single blow:
// Where 2147483647 = 01111111111111111111111111111111 in binary
if( (myInteger & 2147483647) == myInteger )
    // myInteger is BOTH positive and an integer
if( (myFloat & 2147483647) == myFloat )
    // Won't happen
* note bit AND operation is wrapped with parenthesis to make it work in chrome (console)

If you have trouble remembering this convoluted number, you can also calculate it before-hand as such:
var specialNumber = ~(1 << 31);

Checking for negatives
Per @Reinsbrain's comment, a similar bitwise hack can be used to check for a negative integer. In a negative number, we do want the left-most bit to be a 1, so by forcing this bit to 1 the number will only remain unchanged if it was negative to begin with:
// Where -2147483648 = 10000000000000000000000000000000 in binary
if( (myInteger | -2147483648) == myInteger )
    // myInteger is BOTH negative and an integer
if( (myFloat | -2147483648) == myFloat )
    // Won't happen

This special number is even easier to calculate:
var specialNumber = 1 << 31;

Edge cases
As mentioned earlier, since JavaScript bitwise operators convert to 32-bit integers, numbers which don't fit in 32 bits (greater than ~2 billion) will fail
You can fall back to the longer solution for these:
if( parseInt(123456789000) == 123456789000 && 123456789000 > 0 )

However even this solution fails at some point, because parseInt is limited in its accuracy for large numbers. Try the following and see what happens:
parseInt(123123123123123123123); // That's 7 "123"s

On my computer, in Chrome console, this outputs: 123123123123123130000
The reason for this is that parseInt treats the input like a 64-bit IEEE float. This provides only 52 bits for the mantissa, meaning a maximum value of ~4.5e15 before it starts rounding

A: Positive integer:
if (parseInt(values, 10) > 0) {

}

A: To just check, this is the fastest way, it seems:
var sign = number > 0 ? 1 : number == 0 ? 0 : -1;
//Is "number": greater than zero? Yes? Return 1 to "sign".
//Otherwise, does "number" equal zero?  Yes?  Return 0 to "sign".
//Otherwise, return -1 to "sign".

It tells you if the sign is positive (returns 1), or equal to zero (returns 0), and otherwise (returns -1).  This is a good solution because 0 is not positive, and it is not negative, but it may be your var.
Failed attempt:
var sign = number > 0 ? 1 : -1;

...will count 0 as a negative integer, which is wrong.
If you're trying to set up conditionals, you can adjust accordingly.  Here's are two analogous example of an if/else-if statement:
Example 1:
number = prompt("Pick a number?");
if (number > 0){
  alert("Oh baby, your number is so big!");}
else if (number == 0){
  alert("Hey, there's nothing there!");}
else{
  alert("Wow, that thing's so small it might be negative!");}

Example 2:
number = prompt("Pick a number?");

var sign = number > 0 ? 1 : number == 0 ? 0 : -1;

if (sign == 1){
  alert("Oh baby, your number is so big!" + " " + number);}
else if (sign == 0){
  alert("Hey, there's nothing there!" + " " + number);}
else if (sign == -1){
  alert("Wow, that thing's so small it might be negative!" + " " + number);}

A: if (values > 0) {
    // Do Something
}

A: I thought here you wanted to do the action if it is positive.
Then would suggest:
if (Math.sign(number_to_test) === 1) {
     function_to_run_when_positive();
}

A: 1 Checking for positive value
In javascript simple comparison like: value >== 0 does not provide us with answer due to existence of -0 and +0
(This is concept has it roots in derivative equations) Bellow example of those values and its properties:
var negativeZero = -0;
var negativeZero = -1 / Number.POSITIVE_INFINITY;
var negativeZero = -Number.MIN_VALUE / Number.POSITIVE_INFINITY;

var positiveZero = 0;
var positiveZero = 1 / Number.POSITIVE_INFINITY;
var positiveZero = Number.MIN_VALUE / Number.POSITIVE_INFINITY;

-0 === +0                     // true
1 / -0                        // -Infinity
+0 / -0                       // NaN
-0 * Number.POSITIVE_INFINITY // NaN

Having that in mind we can write function like bellow to check for sign of given number:
function isPositive (number) {
    if ( number > 0 ) {
        return true;
    }
    if (number < 0) {
        return false;
    }
    if ( 1 / number === Number.POSITIVE_INFINITY ) {
        return true;
    }
    return false;
}

2a Checking for number being an Integer (in mathematical sense)
To check that number is an integer we can use bellow function:
function isInteger (number) {
    return parseInt(number) === number;
}
//* in ECMA Script 6 use Number.isInteger

2b Checking for number being an Integer (in computer science)
In this case we are checking that number does not have any exponential part (please note that in JS numbers are represented in double-precision floating-point format)
However in javascript it is more usable to check that value is "safe integer" (http://people.mozilla.org/~jorendorff/es6-draft.html#sec-number.max_safe_integer) - to put it simple it means that we can add/substract 1 to "safe integer" and be sure that result will be same as expected from math lessons.
To illustrate what I mean, result of some unsafe operations bellow:
Number.MAX_SAFE_INTEGER + 1 === Number.MAX_SAFE_INTEGER + 2;          // true
Number.MAX_SAFE_INTEGER * 2 + 1 === Number.MAX_SAFE_INTEGER * 2 + 4;  // true

Ok, so to check that number is safe integer we can use Number.MAX_SAFE_INTEGER / Number.MIN_SAFE_INTEGER and parseInt to ensure that number is integer at all.
function isSafeInteger (number) {
    return parseInt(number) === number
    && number <== Number.MAX_SAFE_INTEGER
    && number >== Number.MIN_SAFE_INTEGER
}
//* in ECMA Script 6 use Number.isSafeInteger

A: I use in this case and it works :)
var pos = 0;
var sign = 0;
var zero = 0;
var neg = 0;
for( var i in arr ) {
    sign = arr[i] > 0 ? 1 : arr[i] == 0 ? 0 : -1;
    if (sign === 0) {
        zero++;
    } else if (sign === 1 ) {
        pos++;
    } else {
        neg++;
    }
}

A: You should first check if the input value is interger with isNumeric() function. Then add the condition or greater than 0.
This is the jQuery code for it.
function isPositiveInteger(n) {
        return ($.isNumeric(n) && (n > 0));
}

A: For checking positive integer:
var isPositiveInteger = function(n) {
        return ($.isNumeric(n)) && (Math.floor(n) == n) && (n > 0);
}

A: Starting from the base that the received value is a number and not a string, what about use Math.abs()? This JavaScript native function returns the absolute value of a number:
Math.abs(-1) // 1

So you can use it this way:
var a = -1;
if(a == Math.abs(a)){
    // false
}

var b = 1;
if(b == Math.abs(b)){
    // true
}

A: The Math.sign() function returns either a positive or negative +/- 1, indicating the sign of a number passed into the argument. If the number passed into Math.sign() is 0, it will return a +/- 0. Note that if the number is positive, an explicit (+) will not be returned.
console.log(Math.sign(-3));
let num = -123;
let val = Math.sign(num);
if(val === -1){
    console.log(num + " is negative number");
}else{
    console.log(num + " is posative number");
}

redpajama_set_name: RedPajamaStackExchange


Single-cell level studies are being used increasingly to measure cell properties not directly observable in a cell population. High-performance data acquisition systems for such studies have, by necessity, developed in synchrony. However, improvements in sample purification techniques are also required to reveal new phenomena. Here we assessed a cell sorter as a sample-pretreatment tool for a single-cell level assay. A cell sorter is routinely used for selecting one type of cells from a heterogeneous mixture of cells using specific fluorescence labels. In this case, we wanted to select cells of exactly the same size, shape, and cell-cycle stage from a population, without using a specific fluorescence label.
We used four light scatter parameters: the peak height and area of the forward scatter (FSheight and FSarea) and side scatter (SSheight and SSarea). The rat pheochromocytoma PC12 cell line, a neuronal cell line, was used for all experiments. The living cells concentrated in the high FSarea and middle SSheight/SSarea fractions. Single cells without cell clumps were concentrated in the low SS and middle FS fractions, and in the higher FSheight/FSarea and SSheight/SSarea fractions. The cell populations from these viable, single-cell-rich fractions were divided into twelve subfractions based on their FSarea-SSarea profiles, for more detailed analysis. We found that SSarea was proportional to the cell volume and the FSarea correlated with cell roundness and elongation, as well as with the level of DNA in the cell. To test the method and to characterize the basic properties of the isolated single cells, sorted cells were cultured in separate wells. The cells in all subfractions survived, proliferated and differentiated normally, suggesting that there was no serious damage. The smallest, roundest, and smoothest cells had the highest viability. There was no correlation between proliferation and differentiation. NGF increases cell viability but decreases the proliferative ability of the PC12 cells.
We demonstrated a pretreatment method to collect well-characterized, viable, single cells without using fluorescent labels and without significant damage to the cells. This method is quantitative, rapid, single-step, and yields cells of high purity, making it applicable for a variety of single-cell level analyses.
Recent technical developments have enabled many phenomena to be detected at the single-cell level. For example, improved amplification of RT-PCR now enables single-cell analysis, while the micro-fabrication technique allows better control of cell positioning, morphological analysis, and examination of intercellular contacts between cells [1–3]. Conventional single-cell level measurement techniques such as patch clamping and time-lapse microscopy have also been improved by increasing the number of simultaneous measuring points, thus enabling high-throughput measurement without specialist technical knowledge .
Despite these advances, the simple application of these techniques to a conventional sample is not sufficient. Although heterogeneity within a cell population may have a negligibly small effect on the analysis in population measurements because of population averaging, it becomes prominent in single-cell level analysis. Thus, improvements in sample purification as well as measurement techniques are required for revealing new phenomena. Because a clonal cell line consists of genetically identical cells, it is suitable for single-cell assays. However, properties within a clonal line are not truly homogeneous because of differences in cell cycle, phenotypic variance, and cell damage caused by manipulations. Selection of cells based on empirical knowledge is necessary for meaningful data acquisition at the single-cell level, despite the bias that might be introduced by such a selection process. Thus, a quantitative and rapid sample-pretreatment method of selecting well-characterized and homogeneous cells from a cell line is important for high-quality data acquisition in single-cell assays.
A cell sorter is suitable for the quantitative and high-speed manipulation of individual cells and can be used to separate a specific type of cell based on a composite of flow cytometry (FCM) parameters. A cell sorter is routinely used to measure and sort particular type of cells from dispersed tissue or other mixtures of cell types. Cells can be measured and sorted using both fluorescent labels and light scattering. Measuring the fluorescence of a specific label is useful because labels can be used to amplify cell features. However, the labelling will be different for each application and for each sample. Moreover, labeling is not uniform and may even damage the cells. On the other hand, light scattering reflects general cell properties such as size and shape [5, 6], but is free from the above disadvantages.
In the present study, we applied a cell sorter to select cells of the same size, shape, and cell-cycle stage, based on light scattering parameters. We used both peak height and area (integrated value) of light scatter parameters (Fig. 2), of which only one was generally used to sort cells, and did not use a specific label. We used the rat adrenal pheochromocytoma PC12 cell line, which behaves like neural progenitor cells; the cells proliferate in serum-supplemented media and differentiate into sympathetic neuron-like cells with long neurites upon the addition of nerve growth factor (NGF) . The PC12 cells are easy to handle and culture, can survive when single cells are isolated in a separate well, and neuronally differentiated cells are readily distinguished under the microscope. At first, fractions rich in living cells and single cells were identified. Then, the single live-cell-rich region was divided into twelve subfractions, and the size and shape of the cells and cell phase in each fraction were correlated using light scattering parameters. Finally, to test the methods as well as to characterize the basic properties of sorted cells at a single-cell level, single cells were sorted from each subfraction into individual wells of a 96-well plate to determine the viability, proliferation, and differentiation potential with or without NGF.
Living and dead cell fractions. A and D: two-dimensional profiles of the PC12 cells stained with calcein AM and PI. Calcein-PI (A) and SSarea-FSarea (D, left), FSarea-FSheight (D, middle) and SSarea-SSheight (D, right). Cells without dye (B) and cells permeabilized by 0.1 % saponin with dye added (C) were used to define the dead cells. Calcein-positive and PI-negative cells (living cells) are represented by blue dots and calcein-negative or PI-positive cells (dead cells) are represented by red dots.
Schematic representation of the particle structures, with peak height and area. A: Single cell with uniform content (a), single cell without uniform content (b), and doublet cells with uniform content (c). B: Time course of signals. C: Position of the cells in the height and area profiles. D: A sphere consists of uniformly dispersed small reflectors and has a central part with a slightly different refractive index (see Discussion).
To determine the fractions containing living cells by light scattering, PC12 cells were stained with the fluorescent markers calcein AM and propidium iodide (PI). Calcein AM is a fluorogenic esterase substrate that is hydrolyzed to a green fluorescent product in living cells; thus, green fluorescence was an indicator of cells with esterase activity as well as an intact membrane to retain the esterase products. PI is a red fluorescent nucleic acid stain that is only able to pass through the compromised membranes, thus it marks dead cells. In Fig. 1, each blue dot identifies the calcein-positive and PI-negative cells as living cells, whereas each red dot represents calcein-negative or PI-positive cells, which were assumed to be dead cells.
The fact that fractions containing red and blue dots were clearly separated in the scatter profiles in Fig. 1 suggests that using four light scatter parameter enables better gating out of the dead cell. As expected, the red dots were concentrated in the low FSarea fraction and the low to medium SSarea fractions, which may contain cell fragments. We found that red dots were also concentrated in the upper fraction of FSarea-FSheight profile and especially in the upper fraction of the SSarea-SSheight profile. Because dead cells may contain structures with increased light scattering properties, such as vacuoles or aggregated proteins, the height-to-area signal ratio in this fraction was high (see Fig. 2).
It is difficult to completely disperse cells mechanically to obtain truly single cells; usually the dispersed suspension contains many cell aggregates. Extensive enzyme digestion and trituration may aid in breaking up cell clumps, but will tend to cause cell damage. In addition, small parts of the cells may re-aggregate. When the PC12 cells were dispersed using trituration by pipette in calcium- and magnesium-free phosphate-buffered saline (PBS-), more than half of the cells remained as aggregates (see also ). Filtration and density-gradient centrifugation are easy way to remove big aggregates, and cell aggregates can be discriminated using a cell sorter to gate out particles that have a large peak and/or prolonged (broad) signal. However, these methods cannot be used to distinguish between cell doublets and large single cells. Here, we hypothesized that a cell doublet may exhibit a lower height-to-area signal ratio than a single cell of the same size as shown in Fig. 2. Although the longer axis of the doublet will not always be positioned parallel to the flow, the accuracy of doublet detection can be increased by using both FS and SS, which are at right angles to each other.
We tested this working hypothesis by sorting cells based on the FSarea-FSheight profile or the SSarea-SSheight profile. The nuclei of the sorted cells were then stained with SYTO24, a cell-permeable green fluorescent nucleic acid stain, and the nuclei in each particle were counted. Fig. 3A shows the FSarea-FSheight results. The single cell ratio decreased as FSarea increased. However, even among low FSarea fractions, the lower FSheight-to-FSarea ratio fractions contained larger numbers of doublets. The same findings were also seen in the SSarea-SSheight profiles (Fig. 3B). Although late M phase cells were counted as doublets using this method, this limitation is unlikely to have a large impact on the results. We concluded that our working hypothesis was correct.
We further tested the hypothesis using fixed cells. Ethanol-fixed cells were stained with PI and analyzed for relative DNA content using FCM. Although the scatter profiles of these cells (Fig. 3C) were not identical to that of the living cells (Fig. 1D), the scatter profiles had roughly the same shape. In trace b of the PI profile (Fig. 3C), which corresponds to fraction b of the FSarea-FSheight profile, the first peak at 230 and the second peak at 460 corresponded to the G1 and M cell cycle phases, respectively. However, there were additional small peaks around 650 and 850, which positioned at about three and four times the fluorescence of the first peak, respectively. These peaks may correspond to cell aggregates. In fact, the lower FSheight-to-FSarea ratio fraction (r) contained 17% cells around the two extra peaks, but the higher FSheight-to-FSarea ratio fraction (g) contained less than 2% of cells around the third and fourth peaks. In addition, the cells in the higher FSheight-to-FSarea ratio fraction were in the higher SSheight-to-SSarea ratio fraction (Fig. 3C). We concluded again that our working hypothesis about the single cell fraction and light scattering was correct. These results suggest that single cells can be separated without using a specific dye, as they were abundant in low area-signal fractions and higher height-to-area signal ratio fractions. These fractions were both useful for gating out doublets.
Single and aggregate cell fractions. A, B: Number of aggregates in live cells, which were sorted according to their FS profile (A) and SS profile (B). A: Fifty gates in the FSarea-FSheight profile (left). Particles in each cross-section of gates a-c and gates 1–5 in the FSarea-FSheight profile were sorted. Then, the nuclei per particle were counted, and the number of cell aggregates per twenty-seven particles is shown (right). B: Eleven gates in the SSarea-SSheight profile (left), and the number of aggregates per twenty-seven particles (right). C: FSarea-FSheight (left), SSarea-SSheight (middle), and PI (right) profiles of the ethanol-fixed cells. The green and red dots shown in the SSarea-SSheight profile are the cells within the gate g and r shown in the FSarea-FSheight profile, respectively. Traces g, r and b in the PI profile correspond to gates g, r and b in the FSarea-FSheight profile, respectively. The numbers represent the percentage of cells in regions 1–4.
Based on the results thus far, we determined the fractions containing live single cells in the FSarea-FSheight and SSarea-SSheight profiles (Fig. 4A). Because the shape of gates a and b were almost linear, the four parameters could be represented by two parameters. We chose FSarea and SSarea. Violet dots in Fig. 4B indicate the cells in gates a and b. Twelve gates were then set within the gated FSarea-SSarea profile (Fig. 4B). These twelve gates were represented by their respective, normalized FSarea and SSarea coordinates (FSn, SSn), as shown in Fig. 4C. Live cell ratios based on Fig. 1 for all twelve fractions were more than 80%. The low FSn and high SSn fractions contained slightly fewer living cells than the other subfractions. However, all subfractions were thought to mainly contain living cells. To verify that each subfraction consisted of single cells, the subfractions were counted using syto24 (Fig. 4D). One hundred and twenty cells in each fraction were sorted, and the number of doublets counted. Although a few (less than 4%) doublets or single cells in M phase appeared in the large SSn fractions, their number was small enough to be negligible. These results suggest that the twelve subfractions consisted of single cells.
Living- and single-cell gates and the twelve subfractions. A: Living and single cell-rich gates in FSarea-FSheight (a, blue) and SSarea-SSheight (b, red) are shown. B: SSarea-FSarea profile. Left: dot plot of all cells (black) and cells in the a and b gates (purple dots). Right: contour plot of the cells in the a and b gates. The twelve sorting gates are represented by the green rectangles. C: Schematic representation of the twelve gates. SSarea and FSarea values were normalized to the smallest value and represented as SSn and FSn. D: The number of aggregate cells in the twelve subfractions (n = 120). The SYTO24-stained nuclei in the sorted cells were counted. Shading corresponds to that in C.
We next attempted to correlate light scattering parameters with cell microscopy. The subfraction cells were sorted and floating cells were imaged by phase contrast microscopy to reveal many cell types (Fig. 5A). We focused on three characteristics: diameter (d a ), elongation (e), and surface roughness (P/A) (see Methods).
Cell size and shape of the sorted cells in the twelve subfractions. A: Phase-contrast micrographs of the sorted cells: spherical and small (a); spherical and large (b); oval (c); irregular surface (d). B-D: The diameter (d a , B), elongation (e, C), and surface roughness (P/A, D) of the cells are shown. These values were evaluated from the phase-contrast images of cells (see Methods). Left, average for each subfraction; middle, average for SSn; right, average for FSn. Shading corresponds to that in Fig. 4C. The number of measured cells is shown on each column in B. The right ordinate of B is the relative cell volume (d a 3/d3), where d is the average of d a at (SSn, FSn) = (1.00, 1.24) (arrow head). The relative cell volume at (SSn, FSn) = (2.00, 1.24) (arrow) was about two. Statistically significant for C and D at *P < 0.05; **P < 0.005; ***P < 0.0005. Statistically not significant for B and C at #P > 0.05.
where a and b were the fitting parameters. The a value was 3.2, suggesting that SSn has a linear relationship with cell volume. Actually, the cell volume at (SSn, FSn) = (2.00, 1.24) was about twice as large as that at (SSn, FSn) = (1.00, 1.24) as shown in Fig. 5B. The b value was 6.8, suggesting that FSn has a weaker positive correlation with cell diameter. Elongation (e) also increased with the increasing SSn and FSn (Fig. 5C), but depended more on FSn than on SSn. The e at the largest SSn is larger than at other values. At middle and low FSn, although the d a were quite different, all the e were the same (P > 0.1) suggest that the larger cells are not necessarily the elongated cells. There may be two different mechanisms between volume and elongation.
The roughness of the cell surface, P/A, exhibited different dependencies from the cell diameter and elongation, increasing with SSn but decreasing with FSn. The cell, which had a high P/A value, had bubbles around it (Fig. 5Ad) and appeared non-viable. The Pearson's correlation coefficient for the P/A value compared to the ratio of calcein-positive and PI-negative cells (living cells) in the twelve subfractions was significantly smaller than zero (p < 0.05) also supports the view.
Next, we examined the association between light scattering and cell cycle. Because light scattering profiles have been closely correlated to nuclear morphology [9, 10], this method may also be useful in distinguishing cells in a specific stage of the cell cycle. The PC12 cells in the twelve subfractions were sorted then fixed and stained with PI. The relative DNA contents in the subfractions were measured again by FCM. Fig. 6A shows examples of PI profiles in two subfractions. The lower SSn and FSn subfractions (1.33, 1.24) contained only G1 phase cells but the higher SSn and FSn subfraction (2.33, 1.48) contained many S ~ M phase cells. Fig. 6B shows the percentage of S ~ M phase cells in all subfractions. Although the percentage increased with both increasing SSn and FSn, the percentage at the largest SSn is apparently larger than at other values. This distribution was the same as observed for elongation (Fig. 5C). In fact, the Pearson's correlation coefficient between the ratio of cells in S ~ M phase and elongation was 0.9 (significantly positive at P < 0.0025), indicating that elongated cells are in S ~ M phase and abundant in the high FSn fractions. It is surprising that the Pearson's correlation coefficient between the ratio of cells in S ~ M phase and cell size was not significantly positive (P > 0.05). Although the cell volume at (SSn, FSn) = (2.00, 1.24) was twice as large as that at (SSn, FSn) = (1.00, 1.24), more than 90% of cells in both subfractions were in the G1 phase. One might expect a specific cell volume at which the cells started to synthesize DNA, however this was not the case. The results suggested that PC12 cells in G1 phase are spherical even if the cell volume has increased, and then the cells move into S phase and start to elongate.
Cell cycle of the sorted cells in the twelve subfractions. Live cells were sorted according to the twelve subfractions, then fixed and stained with PI. A: Two examples of PI profile. Upper trace: (SSn, FSn) = (2.33, 1. 48), lower trace: (1.33, 1.24). The left peak corresponds to the G1 phase and the right region corresponds to S, G2 and M phase. B: Percentage of S, G2 and M phase cells. Shading corresponds to that in Fig. 4C.
We performed a fundamental single cell assay to verify our sorting method developed for doing single-cell assays, and to determine the fundamental properties of single cells. The single sorted cells of all twelve subfractions were cultured in separate wells of a 96-well plate and tested for viability, proliferation, and differentiation potential.
The viability (Vb) of cells from all subfractions was higher than 20% and up to 60% in the presence of NGF (Fig. 7). Because it is well known that viability is low with low concentration culture, the observed viabilities were higher than expected. The differentiation ratio of survived cells was less than 3% without NGF but more than 70% with NGF in all subfractions. The survived cells divided more than five times without NGF but less than 3 times with NGF within 13 days. The sorted single PC12 cells behaved normally; they proliferated readily in medium without NGF but proliferation slowed and differentiation into neuronal cells was frequent in medium containing NGF . These results suggest that the cells responded normally to NGF and therefore sorting and single-cell culturing do not introduce serious damage to the cells.
Viabilities of single-sorted cells plated in separate wells. Cell viability in each of the twelve sub-fractions with (B) and without (A) NGF. Left, average for each subfraction; middle, average for SSn; right, average for FSn. Shading corresponds to that in Fig. 4C. Statistically significant at *P < 0.05; **P < 0.005. C: SER versus NGF for each subfraction. The slope of the line is 1.
One might expect a negative correlation between proliferation and differentiation potential in these cells; however this was not the case. The differentiation ratio and proliferation rate were similar in all subfractions. It is notable that viability at subfraction (1.00, 1.24) was significantly higher than for the other subfractions (Fig. 7C). Because this subfraction contained the smallest, roundest, and smoothest cells (Fig. 5), this was an intuitive result. It is also notable that the cells with NGF showed high viability but a low proliferation rate. The VbNGF values were significantly larger than the VbSER values (P < 0.05), and the average VbNGF to VbSER ratio was 1.3 (Fig. 7C). Although the addition of NGF to serum-free medium, in which the cells can not survive, dramatically improves cell viability , the effect of adding NGF to serum-supplemented medium, in which the cells can survive and proliferate, has not been measured. Generally, cell viability assays are performed using mass cultures, and viability factors cannot be distinguished from proliferation factors. On the contrary, our single cell assays can evaluate viability and proliferation independently. We demonstrated directly that NGF increases cell viability but decreases the proliferative ability of the PC12 cells when cultured in the presence of serum.
This study demonstrated a non-staining sample-pretreatment method to select well-characterized homogeneous cells from a cell line for single-cell level analysis. Although a cell line is genetically identical, it is morphologically heterogeneous due to aggregation, cell cycle, and phenotype variance. We used both peak height and area of light scattering parameters to sort PC12 cells into live single-cell fractions, without the use of a specific label. Although it is usually difficult to discriminate between single large cells and small cell aggregates, we successfully separated these classes using the height-to-area signal ratio of both FS and SS. The distinction methods were versatile enough to apply to both living and ethanol-fixed cells. Moreover, this discrimination method is applicable not only to pretreatment steps but also in the data acquisition stage.
We showed that the FSn is closely related to both the degree of cell elongation and the amount of DNA per cell, and that the SSn was proportional to the cell volume. This is in contrast to the commonly accepted association between light scattering and cell morphology that FS represents the size and SS represents internal structure (cf. owner manual of ALTRA). Light scattering profiles are closely related to morphological features including size, shape, surface detail, and intracellular structure of the particle [6, 9, 10, 12]. The dependency, however, varies according to the structure of the particles and the acquisition method applied [9, 13]. The commonly accepted association was based mainly on the analysis of different-size beads or different kinds of hemocyte using narrow angle analysis [9, 10]. However, current machines use wide angles; for example, the FS and SS of ALTRA are the summation of light scattering data from -23° to 23° and from 42° to 138° from the laser axis, respectively. Moreover, we tested an adherent cell line, in which the component cells are almost of the same structure and size. Thus, it is not surprising that our results differed from the accepted association. One possible explanation of our results is that the refractive index of the cell nuclei differs slightly from that of the cytosol and that the cell contains many small particles that reflect light and are distributed homogeneously (Fig. 2D). Diffraction and refraction are the main components of forward scatter and thus are affected by the size of the nucleus, which is roughly in proportion to the DNA content of the cell. On the other hand, reflection from small particles is the main component of side scatter. Because SSn or SSarea is the time integration of side scatter, it is proportional to the number of particles in the cell and as a consequence, is proportional to cell volume.
Light scattering can be used to gain quantitative criteria of cell appearance, which is a good indicator of cell properties. In experiments where data are acquired using a microscope, such as patch clamping, cells must be selected based on appearance to acquire meaningful data. Based on microscopic observation, cell aggregates, dead cells, and less vital cells can be excluded and cells from which data can be acquired, are selected. This empirical selection, however, may introduce an unknown bias to the experiments. Our single-cell assay showed that the roundest and smoothest cells have high viability. This intuitive result suggests that cell selection using light scattering data is consistent with empirical selection based on cell appearance, but without introducing unknown bias.
In the single-cell assay we used the auto-clone option. Because the conventional limiting dilution method is stochastic, there is no guarantee that individual cells are in separate wells. However, auto-clone enabled us to plate a precise number of cells directly into a separate well. Thus, this option would also be useful for single-cell assays of cell extracts, such as single-cell RT-PCR. This option also enabled us to assess cell viability (survival ratio) and cell proliferation independently, which is difficult to achieve in conventional mass culture. Finally, the single-living-cell assay results confirmed that the cell sorting method used in this study caused no significant damage to the cells, as the sorted single cells in the individual wells survived, proliferated and differentiated normally.
We first demonstrated here that the live single-cell can be distinguished from cell doublets or aggregates from the height-to-area signal ratio in the light scatter parameter. We also demonstrated that the cell volume is estimated by SSarea, while the elongation and the level of DNA in the cells is estimated by using FSarea, which suggests that elongation of cell occurs at the late stage of cell cycle. Then we performed a single cell assay using auto-clone option. The single isolated cells survived, proliferated and differentiated normally, suggesting that no serious damage was caused by the method. We also show the relationship among basic properties of cells such as morphology, cell cycle, viability, proliferation and differentiation. Viability of a cell is estimated from the scattering data separately from the proliferation.
These results suggest that using a cell sorter as a pretreatment method for single-cell assay has several significant advantages. First, the light scatter profiles provide quantitative indicators of microscopic cell appearance, thereby reflecting empirical selection without introducing unknown bias. Moreover, fluorescent dyes such as GFP can be included into the technique if required. Second, because the cells can be sorted based on DNA content and fine differences in cell size, cell volume dependent characters, such as whole-cell expression levels and currents, can be measured with low variance. Third, the auto-clone option enables an exact number of selected cells to be plated into separate wells. Finally, all of these treatments can be done at once and is rapid and less invasive to the cells. Thus, cell sorting as used in this study provides a valuable pre-treatment step for single-cell level assays. This method can be applied not only to various types of single-cell assay but also to single-cell level analysis to investigate complex systems, such as the dynamics of multicellular interactions in which a small difference in the initial state can cause a large difference in the final state.
PC12 cells were obtained from the Riken Cell Bank (Cat #: RCB0009; Ibaragi, Japan). PC12 cells were cultured according to the method described by Green et al. . Briefly, PC12 cells were grown on collagen I-coated plastic dishes (Biocoat; Becton Dickinson, NJ, USA) at 37°C in a humidified incubator in an atmosphere of 5% CO2. The normal culture medium consisted of 85% Dulbecco's modified Eagles' medium (DMEM; Invitrogen Corporation, Carlsbad, CA, USA), 10% heat-inactivated horse serum (Invitrogen), 5% heat-inactivated newborn calf serum (Invitrogen), 50 units/ml penicillin and 50 μg/ml streptomycin (Invitrogen), and 4 mM L-glutamine (Invitrogen).
The culture medium was first replaced with ice-cold calcium- and magnesium-free phosphate-buffered saline (PBS-) containing 1 mg/ml bovine serum albumin (BSA), then the cells were detached from the dish by forceful aspiration of the solution through a 1-ml plastic pipette. The cell suspension was then filtered through a 40-μm nylon mesh (Becton Dickinson) to remove large cell aggregates. Preliminary experiments showed that the FCM parameters depended on temperature, therefore all solutions and samples were kept on ice during subsequent procedures. In experiments that included cell staining, fluorescent dyes were added to the suspension at this point. Preliminary experiments showed that the FCM profile drifted about 20 minutes after harvesting and became relatively stable thereafter. Thus, the suspension was kept ice cold for 30 minutes after harvesting, and then FCM acquisition and cell sorting were performed within 40 minutes using an EPICS ALTRA (Beckman Coulter, Inc., Miami, FL, USA). All measurements were made using an argon laser at 488 nm. The beam size at the focal plane was 6 μm in height and 100 μm in width. The cells were sorted according to four parameters, which comprised the peak height and area of forward scatter (FSheight and FSarea) and side scatter (SSheight and SSarea). The peak shape (peak width or time of flight), which is the transit time of a particle in the laser beam plane, is also a basic parameter. However we did not use the peak shapes because it was sensitive to the operation and was relatively unstable. PMT 2 was used to measure the SS because it had both area- and peak-value output.
The cell sorting settings were as follows: flow chip diameter, 100 μm; sheath solution, IsoFlow (Beckman Coulter); sheath pressure, 8.8 – 9.2 PSI; crystal frequency, 19.0 – 21.5 kHz; crystal drive, 15–30%; delay, 25 – 32 drops; sort mode, 3; coincident abort, on; and pulse pile up (PPU) sensitivity, 4–10 μs.
To evaluate the size and shape of the cells, phase-contrast images were captured using a digital camera (Coolpix 995; Nikon, Tokyo, Japan) placed on an inverted microscope (TS100; Nikon). After importing the images to a computer, an outline of each cell image was traced by hand. The perimeter (P), area (A), and the major and minor axes of the best-fitted ellipse (d maj and d min ) for the outlined cells were then measured using image analysis software (Scion Image; Scion Corporation, Frederick, ML, USA).
To estimate the cell diameter, d maj , d min and the equivalent circular diameter ( d p ≡ P 2 π MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacqWGKbazdaWgaaWcbaGaemiCaahabeaakiabggMi6oaalaaabaGaemiuaafabaGaeGOmaidcciGae8hWdahaaaaa@3554@ ) and equivalent area diameter ( d a ≡ A π MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacqWGKbazdaWgaaWcbaGaemyyaegabeaakiabggMi6oaakaaabaWaaSaaaeaacqWGbbqqaeaaiiGacqWFapaCaaaaleqaaaaa@3441@ ) were used. However, because these four values had a strong correlation (Pearson's correlation coefficients were greater than 0.96), only d a is shown as data. To quantify the cell shape, ellipticity ( e ≡ d m a j d min MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacqWGLbqzcqGHHjIUdaWcaaqaaiabdsgaKnaaBaaaleaacqWGTbqBcqWGHbqycqWGQbGAaeqaaaGcbaGaemizaq2aaSbaaSqaaiGbc2gaTjabcMgaPjabc6gaUbqabaaaaaaa@3B09@ ) and the perimeter to surface area ratio ( P / A ≡ d p d a MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacqWGqbaucqGGVaWlcqWGbbqqcqGHHjIUdaWcaaqaaiabdsgaKnaaBaaaleaacqWGWbaCaeqaaaGcbaGaemizaq2aaSbaaSqaaiabdggaHbqabaaaaaaa@3757@ ) of the cells were calculated. In the case of a circle, e = P/A = 1, whereas e and P/A inequality give e ≥ 1 and P/A ≥ 1. In general, e is an indicator of the degree of elongation and P/A is an indicator of cell surface roughness.
To determine the fractions containing living cells, 100 nM of Calcein AM (c3100; Molecular Probes, Inc., Eugene, OR, USA) and 100 ng/ml of propidium iodide (PI, A4378; Sigma-Aldrich, St. Louis, MO, USA) were used. Calcein and PI signals were measured using a 525-nm bandpass filter and a 675-nm bandpass filter. Because the Calcein and PI fluorescence overlap was negligible, the fluorescence signals were not compensated.
To counting nuclei, the live cells were stained by 5 μM of Syto 24 (S7559: Molecular Probes, Inc).
To assess the cell cycle stage, total DNA in each lot of PC12 cells was measured using FCM. The cells were suspended in 300 μl of ice-cold PBS- and 700 μl of -20°C ethanol was added to the suspension while vortexing gently. The fixed cells were stored at -80°C. The cells were washed in PBS- and re-suspended in PI staining solution containing 20 μg/ml of PI, 0.1% (v/v) Triton X-100, and 200 μg/ml of DNase-free RNase in PBS-. The cells were incubated for 30 minutes at room temperature and were kept ice cold before FCM acquisition. PI signals were measured using a 675-nm bandpass filter.
PC12 cells were divided into fractions according to the twelve-subfraction separation (Fig. 4C). Single cells were plated into the individual wells of a 96-well plate using the auto-clone option of the ALTRA cell sorter. The success rate for plating one cell per well was more than 90%. Each well was precoated with collagen I (Iwaki, Tokyo, Japan) and contained 100 μl of normal culture medium.
Two day after sorting, half of the wells were given an additional 100 μl of culture medium containing 100 ng/ml of NGF without serum, and the other half were given culture medium with serum. Thus, half of the wells contained 50 ng/ml NGF, 5% HS and 2.5% NCS ("NGF" condition), and the other half contained normal culture medium ("SER" condition). Half of the culture medium was changed every 2 to 4 days. The cells were cultured from eleven to thirty days after sorting. Cell viability (Vb) or survival ratio was the ratio of the number of wells containing any cells to the number of single-cell plated wells. The differentiation potential for the surviving cells was the average ratio of neurite-bearing cells per total cells in a well. The proliferation potential for the surviving cells was the logarithm of the average cells in a well to the base 2.
This work was supported by a research fellowship (13–83203) from the Japan Society for the Promotion of Science for Young Scientists, a 21st century COE grant from the Japan Society for the Promotion of Science. The authors would like to thank Mr. Yasuhiko Nagasaka from Beckman Coulter Inc. for his technical assistance and helpful advice regarding flow cytometry and cell sorting.
KO and TY designed the included experiments. KO performed all of the experimental work and drafted the manuscript. TY, KK, and MA coordinated and supervised the study. All authors read and approved the final manuscript.


I've always wanted to make my own chocolate hazelnuts spread and here it is. Making it at home lets you control the content of sugar and of hazelnuts which is important as the kids love it and want to give them the best.
This is a more intense version of Nutella made at home. Tastier, healthier and absolutely delicious. If served with homemade bread it's even better.
Process nuts in a food processor for about 5 minutes until you get a smooth liquid butter texture.
Meanwhile melt chocolate over a bain-marie until smooth. Add the melted chocolate, sugar, cocoa powder, oil and vanilla extract to hazelnuts and process until smooth.
Transfer the spread into a small sauce pan and cook at 50 C (very low temperature) for 5 minutes then place finished hazelnut chocolate spread to an airtight container, let cool then refrigerate. Stir before serving.
Nutrition facts Whole Spread - Calories:2045, Fat:146.4 g, Saturated Fat:53.4 g, Carbohydrates:166.4 g, Sugar:138.0 g, Fiber:15.9 g, Protein:27.6 g, Cholesterol:46 mg, Calories from Fat 1318, Sodium 160 mg, Vitamin A 8%, Calcium 47%, Vitamin C 8%, Iron 50%, Nutrition Grade D, Percent Daily Values are based on a 2,000 calorie diet.
This looks so yummy! I've been looking for this recipe for awhile!


First draw a long and straight line that will pass through the center of the entire sword. Claymore is a long sword, so this line should be quite long. Claymore is a long sword, so this line should be quite long.... When you purchase a sword from Cold Steel, you're buying more than just "a sword", you're investing in hand crafted beauty along with detailed, authentic reconstruction of a sword from a specific time period. Cold Steel swords provide an elegant, hand crafted look and feel you just can't find anywhere else. Made from High Carbon steel, Cold Steel swords are an excellent addition to any collection.
A sabre (British English) or saber (American English) is a type of backsword with a curved blade associated with the light cavalry of the early modern and Napoleonic periods.... Manual of Arms--Saber and Sword: General The nomenclature for the saber is saber, officer, infantry, revolutionary. The blade is 31 inches long. The nomenclature for the sword is sword, infantry, Army, noncommissioned officer, model 1840.
As a gang of robbers try to break in through a shop window, this group of jewellery shop workers quickly arm themselves with long blades to drive the would-be thieves away. how to become c tpat certified A sabre (British English) or saber (American English) is a type of backsword with a curved blade associated with the light cavalry of the early modern and Napoleonic periods.
Is it a "Sword" or a "Saber"? Marlow White Uniforms Inc.
This is the position before the command Draw, SABER (SWORD) and after the command Return, SABER (SWORD). The hands are behind the trouser seams and the thumbs touch the first joint of the forefingers ( Figure - 2 ). how to clear off the passwords that appear on gmail When you purchase a sword from Cold Steel, you're buying more than just "a sword", you're investing in hand crafted beauty along with detailed, authentic reconstruction of a sword from a specific time period. Cold Steel swords provide an elegant, hand crafted look and feel you just can't find anywhere else. Made from High Carbon steel, Cold Steel swords are an excellent addition to any collection.
When you purchase a sword from Cold Steel, you're buying more than just "a sword", you're investing in hand crafted beauty along with detailed, authentic reconstruction of a sword from a specific time period. Cold Steel swords provide an elegant, hand crafted look and feel you just can't find anywhere else. Made from High Carbon steel, Cold Steel swords are an excellent addition to any collection.
This is the position before the command Draw, SABER (SWORD) and after the command Return, SABER (SWORD). The hands are behind the trouser seams and the thumbs touch the first joint of the forefingers ( Figure - 2 ).


Heather Fleming presented an example of her company's partnership with Inovations for Poverty Action to improve hand sanitation in Kenya.
Heather Fleming, founder and CEO of Catapult Design, gave a lecture Monday night that moved students in the room to take notes on their phones.
Raised in Vanderwagen, New Mexico, less than a mile from the Navajo reservation, Fleming stressed the role of engineers and designers in addressing the inequities she witnessed growing up.
Fleming is the third speaker to be featured at the Dana M. Dourdeville Lecture on Engineering in Service to Society, which was established in 2015 to feature the "profound impact science and technology can have on society," said Dean of the School of Engineering Lawrence Larson.
Fleming highlighted the correlation between dramatic population growth and poorer nations to show the necessity of engineering in global development. She pointed to entrepreneurs who helped Rwandan women stay in school with a low-cost, locally-manufactured sanitary pad as an example of a design initiative addressing humanitarian challenges.
People-oriented problem solving first attracted Fleming to engineering when her cousin created a water tank that gave running water to those without it.
After graduating from Stanford, selling flowers for a year and working with remote controls and 3D TVs, it was through Engineers Without Boarders that Fleming rediscovered a link between engineering and humanitarianism.
"After a year of stumbles, we incorporated Catapult Design to continue what we learned in our day jobs as product design consultants — and our night and weekend jobs as volunteers — to try to tackle these global development challenges," she said.
Nine years later, Catapult Design has taken on 60 design projects in 14 different countries to make design accessible to organizations working to solve global, humanitarian problems, she said.
Fleming walked audience members through Catapult Design's partnership with Innovations for Poverty Action to improve hand washing in rural Kenya. The presentation featured video clips that showcased Fleming's trip to Kenya, conversations to identify how people think about and use soap, meetings with stakeholders and manufacturers, prototyping and the end product — a functioning, durable hand-washing system.
Fleming also observed little available information surrounding how to start a business. To address the gaps in knowledge, she followed the trajectory that Catapult Design had established. After a process that included many conversations with the few entrepreneurs they could find, Fleming and her team prototyped "Build Navajo" in December 2016, she said. The website visually illustrates the process of starting a business, as detailed by successful Navajo entrepreneurs.
Nadia Goldberg '21 said the talk has changed the way she thinks about her career.


The Dutch precision technology community is built on cooperation and communication. In order to allow the sharing of the available expertise and experience in the field of precision and control technology to continue, DSPE (Dutch Society for Precision Engineering) organizes the DSPE Conference on Precision Mechatronics every two years. The fourth edition is scheduled for 4 and 5 September 2018. The theme for 2018 is 'Precision Imagineering', the combination of precision, 'imagination' and 'engineering'. A company can start with a dream or imagination, but engineering skills in the broadest sense of the word are needed to actually turn an early idea into a successful product, service or company. The deadline for submission of abstracts is February 1, 2018.
This year's theme 'Precision Imagineering' stands for the combination of Precision, Imagining and Engineering. It is inspired by the discussion in the Advisory Board that any successful business starts with a dream or with 'Imagination' but it takes 'Engineering' skills in the broadest meaning to actually transform the initial idea into a successful product and business. When it comes to precision engineering products it is evident that one needs to master the various aspects of precision engineering (design principles, dynamics, control, thermal aspects, contamination, …) in great depth. But the development of modern mechatronic devices, like IC equipment, electron microscopes, harvesting robots or medical robots for eye surgery, also requires mastering the art of managing complexity by applying systems engineering and having a keen eye on the growing importance of software and the possibilities and challenges that new developments such as artificial intelligence and deep learning offer to the precision engineering community.
In line with the above DSPE strive to extend the traditional core topics of the conference with a session about software and systems engineering aspects in the development of precision equipment and the conference will be open to members of the SASG (System Architecting Study Group). Also they will select the guest speakers in line with the conference' focus. With three guest speakers, 21 oral presentations and hopefully many posters and demonstrations, and a social event there will be plenty of food for thought, discussion and networking.
Areas of interest include (but are not limited to): pm-stability, disruptive technologies & approaches, additive manufacturing, topology optimization, new materials, design principles, sensors, actuators, metrology, imaging technology, thermal design, vacuum & contamination, motion control, dynamics & acoustics, reliability, energy efficiency, machine specs vs. user needs, new business, value creation, open innovation.
The 4th DSPE conference on precision mechatronics, which will take place on September 4-5, 2018 at the inspiring conference location De Ruwenberg in Sint Michielsgestel, The Netherlands.


A key which uniquely identifies a discovered object in the CommandCentral Storage database. OIDs are represented in XML files as hexadecimal strings with a maximum length of 128 characters. Also called object reference.


Never mind 2012, what impact will U.S. Latinos have in 2016?
I think that the GOP is going to take back Congress in November. And fast forwarding to 2012, it is becoming entirely plausible that an ineffective President Barack Obama will step aside and allow U.S. Secretary of State Hillary Clinton to try to hang on to the White House.
I have no idea how 2012 will pan out. It may well be that the GOP will rebound during 2010-12 and seize back the White House. That could happen. The bigger question is, what affect will the GOP's bashing of Latinos do to the Republican Party not in 2012, but rather in 2016? With, or without, a new amnesty, the future is looking quite grim for the GOP.
Think about it. Latino children who are 12 right now will be 18 in 2016. Many if not most of these kids are American citizens. How do you think they will be voting?
There are significant and increasing demographic ties throughout the Western Hemisphere. Today, approximately 15 percent of U.S. citizens claim Hispanic heritage. By the middle of the century, this could easily rise so that nearly one quarter of the population of the United States will trace its heritage to Latin America and the Caribbean.
More than 500 million people – one half of the hemisphere's population – live in Central and South America and the Caribbean. United Nations statistics show that in 2000, Latin America and the Caribbean consisted of 8.6 percent of the world's population. While this percentage is expected to remain static, the actual population in real numbers may grow to 768 million by 2050.
While the U.S. is thought of as primarily an English-speaking nation, it is also the 5th largest Spanish-speaking country in the world.
And what does the U.S. Census think? Well, take a look at the graphic atop this post. That graphic shows U.S. population growth. Check out how fast the Latino portion of our population is going to grow compared to everyone else!
The United States will be majority-minority nation by 2042. By 2050, the country will be 54 percent minority as Latinos double from 15 percent to 30 percent of the population, Asian Americans increase from 5 percent to 9 percent, and African Americans move from 14 to 15 percent.
Unaffiliated or secular voters—not white evangelical Protestants—are the fastest-growing "religious" group in the United States. The percentage of adults reporting no religious affiliation almost tripled from 1944 to 2004, rising from 5 percent to 14 percent. Projections indicate that by 2024, 20 percent to 25 percent of U.S. adults will be unaffiliated.
This trend—combined with growth among non-Christian faiths and race-ethnic trends—will ensure that by the 2016 election (or 2020 at the outside) the United States will have ceased to be a white Christian nation.
Here is the bottom line – the GOP may win in 2010, and in 2012, but after that, it is over for them, period. Unless of course they change their ways and stop bashing minorities, gays, etc. Yes, fat chance of that.
I know this will be hard for our Republican readers to swallow. I have been warning your party for years. Enjoy your victories in November and perhaps even in 2012. They will be your last.
Bennett, who was kicked off his party's primary ballot last month at the Utah GOP convention, predicted that Republicans will retake control of the House of Representatives in November, but suggested that the shift in power may be short-lived given the lack of solutions coming from their side of the aisle.
"The concern I have is that ideology and a demand for absolute party purity endangers our ability to govern once we get into office," Bennett said.
think of the harm they could cause during the final 2, 4, or 6 years of their death throes. I don't want to.
What may surprise our readers is that PPIC found that more Latinos are conservative than liberal.
Therefore, their support should not be taken for granted by the "progressive" Democratic Party.
They're conservative on social issues. They're generally more religious, against abortion, less accepting of gay rights etc. But they're progressive as far as economics go, and REALLY what do you think could EVER entice many of them to vote for the Latino-bashing Republicans?
One thing you can say about "W" is that he at least made an attempt to reach out to the Latino community. As stated previously, Ron Winship and I covered his presentation at the Ontario Convention Center in Jan. 2002 that was sponsored by several Latino business groups who spoke highly of his inclusion of Hispanics.
We also covered (and interviewed) U.S. Treasurer Rosario Marin in July of 2002 as the White House promoted the No Child Left Behind Act at Huntington Park H.S. with a focus on having Hispanic students excel in the public school system so that like Rosario's example, could make a better life for themselves.
She was born in Mexico City and has experienced the American dream.
You want a true Latino role model? She's the ticket.
Rosario, an illiterate immigrant, is the first Mexican born citizen to head up the U.S. Treasury Department. In fact she is the only foreign born Treasurer of the USA.
I know Rosario very well. When I worked in Latino outreach for your party I worked with her and many other Latinos who went on to work for Bush and Schwarzenegger.
She however got busted for charging travel expenses to her agency, that she incurred when traveling to go get paid to speak at a function. That was, as one might expect, no bueno. She had to resign. I am not sure what she is doing now.
Art. Sharing the stage with Rosario was Grammy recording artist winner Jon Sekada who shared with us his life as an immigrant who grew up in the streets of Miami. He succeeded and attributed education as being a big part of his success. He told us that he looked up to his teachers, went to college, and was aware of the challenges yet kept his traditions and language alive.
Brother Vern. My friend. This national tour was during a Republican administration.
We were told that $11.5 billion was being spent on Title I. Therfore I cannot say if this endeavor became another unfunded mandate or not.
Note: Rosario Marin lost to Bill Jones in the 2004 primary race to challenge Barbara Boxer. We all know the outcome of that election as once again Republicans put another candidate into the Senate race arena.
Do you know how silly you sound, going on and on about one hispanic Republican lady, one exception to the rule? Stop.
There were several messages in those comments starting with her being an illiterate immigrant from Mexico City yet climbing the ladder of success.
The same applies to the value of getting a good education and learning the English language if you intend to get a well paying job. Oh, I didn't add those comments from that event .
Vern. Forget for the moment the impact of future election participation. With almost 50% drop out from some of our high schools there is a much bigger issue that Hispanic's must recognize and address.
Brother Vern. The impact of Hispanics in 2016 is quite simple. Don't wait. Register to vote as Republicans and cast your votes for Carly as Barbara needs to return to Fisherman's Wharf.
I grant you, that's one of the few issues W was good on.
On the other hand, so was John McCain and Lindsey Graham back then.
This decade's GOP is anti-immigrant, anti-Latino, anti-hispanic, anti-illegal, whatever. Believe me, it's all the same to them, they know it's a racial thing.
Things always change with time, and no matter how much things change, it always seems to remain the same.
In the future, the possible Latino majority will be the new Republican majority of the Democratic party.
I think he might be saying that Latinos will be the majority of the Democratic party – which sounds true enough – and that they will make the party more conservative on social issues – which may also be true.
Except really, I think it's more older Latinos that are hung up about gays and abortion, the future is more with the younger Latinos who are hip and open-minded.
on purpose, just like with this holding back and stereo typing the unemployed.
for unemployment checks and so many of you are now standing on street corners with signs" You're being "laughed at", by us "it's the brown/black people!
we will not only enjoy our victory, we will make sure you hate it. all trends can be reversed, perhaps immigration should have some diversity, you know, people who are not Latino. How about more whites or Vietnamese. What will happen to la raza then? hopefully the four years after our victory will bring about immigration changes that will not only stop la raza but dilute them, boot them, and moot them.
dmw why do you think far left vern and the other libs want them . they know they cant vote ..the majorty of ILLEGAL immigrants that come here are poor low skill THEY WANT THE FREE HANDOUTS . they want to give them amensty so they can vote for dems . .the dems are a party of handouts , freebies , since they come here with nothing they will vote for them IF WE LET THEM . this will keep them in power . they are always yelling they want comprehensive immigration reform =aka amensty . our laws are fine THEY ARE NOT ENFORCED that is the problem .
Read the post. Click the links. It is game over for the GOP with or without a new amnesty. Sayonara haters!
Read the report. It is already too late. Unless the rest of you start breeding like rabbits, it is game over.
Mexicans are not Latinos! They are descendants of Aztecs or Mayan Indians. Mexicans should not be referred to as Latinos. Here in Orange County, when they say "Hispanic" or "Latino", it is readily accepted as Mexican. People need to be more educated about the heritage of various peoples within the North South and Central American regions. Mexicans are not Latinos!


text:
Home > Club Info > Meet Our Sponsors
Club Info Juniors
28th June 2017 5th September 2018 Storm Netball Club1458
Storm is proud to be associated with some terrific sponsors who enable us to continue to push the club forward.
Sponsorship is a vital part of the club achieving its goals and can be a fulfilling role for a business to work alongside an ambitious and forward thinking club like Storm.
We are very lucky to be associated with the following companies. Please click on the links to go direct to their website. And if you'd like to join our sponsorship team please get in touch via our contact page.
MINING SEARCHES UK
www.miningsearchesuk.com
Mining Searches UK became a Storm sponsor at the start of the 2018/19 season.
The company has taken sponsorship of the club's Senior match dresses, training skins, Coaching Jackets and Warm Up Shirts for the entire Junior and Senior sections.
KHK Media
www.khkmedia.co.uk
Storm's Senior and U16 Team play in KHK Media sponsored dresses after the photography company run by Andrew Trenoweth agreed a new sponsorship deal at the start of the 2012/13 season.
No stranger to the club, Andrew started working with Storm in 2008. Then In 2009 KHK Media sponsored the team with a set of match balls, and in 2010 become a main team sponsor providing the senior teams with match dresses and team jackets for the support staff.
In 2012 they have continued their commitment to the club by helping to provide new Under Armour match dresses for the four senior teams and U16 Tornadoes.
"I am delighted to continue working with Storm," Andrew said. "The club has grown incredibly in the last few years and it is a pleasure to be able to help Storm continue to push forward. I take great pride in seeing the players and officials look so professional and I think it has really helped raise the profile of Storm Netball Club."
As part of the sponsorship deal KHK Media will continue to supply the club with match and social event photography and it's something Andrew has has enjoyed doing for the past five seasons. "The last few years have been amazing and I've been able to experience some incredible matches and events first hand," he said.
Philosophi – Experience Digital
http://www.philosophi.uk/
We're delighted to welcome aboard for three seasons from 2016, Philosophi as one of our main Junior team sponsors.
Philosophi is a results driven, digital marketing agency focusing in the B2C retail and professional services sectors. They have a wealth of experience, a highly skilled team and happy satisfied clients.
They will bring you creativity, innovative solutions and value for money. The team will strive to get you the very best return on your marketing investment and can help plan, deliver, monitor and report on any digital marketing campaigns.
Storm is thrilled to be working with Philosophi who will be the club's U13 & U12 match kit sponsors for the Cornwall Junior League from the 2016/17 season.
Tagged Sponsorship
South West Fixtures Are Out
Storm Netball Club
Storm Netball Club is one of Cornwall's premier sports clubs. Playing in the South West Regional League at Senior level with teams also in the Cornwall Senior & Junior Leagues.
http://www.stormnetballclub.com
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This is a Book Club thread for Elminster Must Die (part of "The Sage of Shadowdale" saga), by Ed Greenwood. Please discuss the Prologue and chapters 1 - 5 herein.
Hi, Sage. I'm not sure if Wizards is still regarding ELMINSTER MUST DIE! as Book 1 of "The Sage of Shadowdale" trilogy.
In the published tome, the entire saga of Elminster now seems to be headed "The Sage of Shadowdale" and all "Book 1" mentions have been expunged.
Ed has a 6-book contract, so I'm not sure it's a trilogy.
Ed, of course, can't say - - not just legally; I don't think he KNOWS.
I appreciate the clarification, milady. I was working from old data on the Wizards Novel Page. I'll make the necessary adjustments.
Indeed, it looks as though Sage of Shadowdale may be a blanket title for the entire tale of Elminster, all the way back to the beginning. Elminster Ascending bears the title as well, indicating that Sage of Shadowdale may [finally] be giving old El an umbrella grouping the way that The Legend of Drizzt indicates tales about the drow ranger and his comrades.
Nice little nod to our own Garen Thal.
I haven't gotten the book yet, but from where I'm sitting, there's nothing 'little' about it. Knowing what Ed means by it (both because he forewarned me it would be in the book, and because he's told me more directly), it's a compliment of the highest order, and well-appreciated.
Sage, Maybe we need a scroll just for all the easter eggs that our fellow scribes can spot!
I'll decide on that once I finally receive my copy.
Ok, where do we start.
The Steel Regent. And with a snarky, disrespectful utterance of Vangey's name to boot.
What more could a Greenwood fan mask for?
I haven't read this book yet, (still waiting for the shipment) but one quick question: (Ed's novels usually involve the cockroach Manshoon and far too many Zhents, whom I really tried to like or at least spare a mote of liking but always failed to.) Does this book feature them again ? Or are there other equally strong or greater power groups that desire and scheme for the ultimate demise of our beloved El?
The novel features an old foe, High Knights galore, an arrogant War Wizards.
All who wish to kill El.
I could be wrong, but it seems Manshoon has been in less than half of Ed's Novels. I understand you do not like him....so why do we need to see some variant of "that cockroach Manshoon", which your posts usually feature?
And without spoiling later parts of the novel...this is not the same "manshoon" ,in the same ways Ed has been asked to write about in the past(bumbling, inept almost comical and not near evil enough). He is much more like what Ed's original charcter is.
remember this, always, when regarding Ed's or anyone else's Realms novels: they are all "work for hire." Authors (yes, including Ed and Bob Salvatore) write about characters they are told to write about, not "whoever they may feel like" writing about.
As for your query: if there are any Zs included, that's not confirmed to the reader. Yes, "M" appears. One of the themes of the book is the manias/toll of surviving for centuries and using/abusing/overusing/becoming ravaged by magic. El and the other Chosen aren't the ONLY characters affected by this, that Ed is comparing and contrasting in the book.
Nor is this the only game Ed is playing, in EL M D!
Heh. Wait'll you see BURY ELMINSTER DEEP . . .
remember this, always, when regarding Ed's or anyone else's Realms novels: they are all "work for hire." Authors (yes, including Ed and Bob Salvatore) write about characters they are told to write about, not "whoever they may feel like" writing about.As for your query: if there are any Zs included, that's not confirmed to the reader. Yes, "M" appears. One of the themes of the book is the manias/toll of surviving for centuries and using/abusing/overusing/becoming ravaged by magic. El and the other Chosen aren't the ONLY characters affected by this, that Ed is comparing and contrasting in the book.
Emphasis mine..that's what I was clumsily trying to convey!
The fact that this book is "about" El is more than enough reason for me to read it. It's just that I hate annoying surprises...I'd undoubtedly be annoyed if I suddenly read the name of the cockroach in this novel without knowing before hand. At least by knowing what to expect, I'd be ready with my insect repellent spray!
That I know. You mentioned that in one of your replies to my query in Qs for Ed. The purpose of my question is only to know what to expect, because I don't like annoying surprises, and the cockroach's sudden presence in any book is nothing but. Besides, my insect repellent spray is not unlimited; I'd have to order more from Japan.
dennis, there's nary a Zhent in sight, except Manshoon. Unless some of the nobles or other attendees at the feast or on the Palace staff are secretly Zhent agents, of course (and if they are, it's not even hinted at). Some characters are Manshoon's agents/pawns/mind-controlled dupes, but that's a different thing.
And from the tone of Ed's writing, I'd say he's hinting that at least one of those agents is going to break free/get out of hand, in a later book.
Ah, this book is FUN. It's got me excited about the Realms again, and I didn't think that was possible . . .
dennis, there's nary a Zhent in sight, except Manshoon.
My worry, exactly. As I love him so much! ...Fortunately for me, no cockroach (yet) is strong enough to thwart me from reading this book.
One of the worst written books that I've ever had the misfortune of reading. What pisses me off more is that I actually had to drive to the book store and buy it since it isn't available in Kindle format! Such an utter waste of time/money.
Why use 50 sentences when 1 would have done? Now everything needs 50,000 moronically written descriptions. My eyes were bleeding after reading the same old stupid quotes like "For the good of the realm" or "I am an agent of the crown, you will obey me"...then 2 seconds later the person dying. The book really made no sense at all. This book is literary vomit.
....No offense but this really is the worst FR's book in history.
Well, now I am actually looking forward to reading the book, that review made it seem like a book I would like. The longer sentences and longer descriptions the better.
Great book thus far, great beginning.
Though I suspect, having read previous Ed books, the fighting will slacken for a bit as we get more conversation and coloring in the characters.
I'm delighted with my purchase, and will be reading right along to the end, expecting more superb entertainment. And I DO expect it to make sense. It being Ed, there will be subtleties and hints, not everything stated baldly...but then, that's what I'm expecting and hoping for.
I'll check in again when I get to the end.
Another enjoyable read from Ed's pen. These opening chapters are the most "gamer-ish" (I suppose another possible term would be "Salvatore-like") in that they feature more "don't make me fight you"/sneer/WHAM big fight" scenes than the rest of the narrative.
Looking across the current living and actively-writing SFWA membership, I count Ed in the top handful of a group of writers that wins few awards and writes few "ground-breaking" or seminal works, but instead tells good yarns that invite re-reading. Of the dead writers in this group, I can readily name Randall Garrett, E.E. "Doc" Smith, James H. Schmitz, and H. Beam Piper . . . but the living writers in this group are headed by Joe Lansdale, Christopher Moore, and Ed.
For Realms fans, this book is more than a solidly enjoyable re-read. It "gives back" a living, interesting, colorful Cormyr to the fans, introduces some great new characters whom I hope we'll be seeing a lot more of, and showcases some old favorites in ways that make it clear to everyone that they're still "in play," very much on the table.
Ed delivers. Well worth the money, and one more step of improvement in his Realms books. Since he started the Knights trilogy, each successive book has been better written. This one is rushed at the end (not that such pacing doesn't fit the tale well, mind you); I look forward eagerly to the sequel, and (despite having turned out more than a dozen sf and fantasy novels of my own, over two decades) energetically envy anyone who can write this well. His dialogue is deft when it doesn't sparkle.
So, I'll dine on this particular literary vomit, with sauce.
(Wince) Now THERE'S a visual.


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text:
SAN DIEGO COMIC CONVENTION ANNOUNCES
WONDER WOMAN AS THIRD INDUCTEE INTO THE
COMIC-CON MUSEUM CHARACTER HALL OF FAME
Virtual Ceremony to be Held
HERE at 4:00pm PT October 21, 2021
In celebration of her 80th anniversary, San Diego Comic Convention (SDCC) has announced that Wonder Woman will be the third inductee into the Comic-Con Museum Character Hall of Fame.
Recognizing the Amazonian princess as a character that embodies the strength and power of the female spirit, she has become a universal symbol of truth, justice, and equality. She has been headlining stand-alone comics since the 1940s, and with her iconic magic lasso she would go on to capture the hearts of countless more fans via her hit 1970's television series as well as roles in animated shows and movies. The groundbreaking 2017 film Wonder Woman by director Patty Jenkins and starring Gal Gadot, was the largest grossing live-action film ever for a solo female director at the time of release.
"Wonder Woman is the perfect inductee into the Comic-Con Museum Character Hall of Fame," commented David Glanzer, spokesperson for SDCC. "Her incredible 80-year legacy and influence on popular arts and culture cannot be denied and may even be more significant today than any time in her long venerated history."
The virtual induction ceremony will allow fans from all over the world to watch the festivities online on October 21, at 4:00pm PT HERE.
The Comic-Con Museum Character Hall of Fame recognizes the value and influence of iconic characters who have made contributions of significant impact on comics and related popular art. Wonder Woman joins last year's inductee PAC-MAN, and Batman, the Museum's first honoree.
Previous MCHOF Inductees
2020: PAC-MAN™
The Comic-Con Museum is thrilled to announce PAC-MAN™ as the second inductee into the Comic-Con Museum Character Hall of Fame.
Born in 1980 and widely considered the original digital game mascot, PAC-MAN™ made a profound impact on the video game industry, the role of storytelling in games, and popular culture as a whole.
For an entire generation, PAC-MAN™ ignited a love of video and arcade games. Those of us who were there for the start of PAC-MAN™ remember going to the arcade and lining up for a chance to play while our friends stood around and cheered on. We were able to connect with PAC-MAN™ on an emotional level in a way we hadn't with other video game characters.
For the generations that followed, PAC-MAN™ is an icon and one of the most widely recognized images on the planet. He plays a significant role in the history of video games, and his presence continues to pervade not only in the game industry but in all areas of popular culture today including fashion, film, food, music, and art.
By honoring PAC-MAN™ we pay homage to digital creators and the vision and values that inspire them. PAC-MAN's™ creator, Toru Iwatani, had no training as a designer or programmer, but he had a powerful vision. A true pioneer and champion for inclusivity, Iwatani aimed to design a game that would appeal to female players. With the creation of PAC-MAN™, Iwatani helped to open the doors of the gaming industry to include all players while also helping creators to understand the opportunity they have to not only make a fun game, but to make a positive impact as well.
As we reflect on 40 years of PAC-MAN™, we are grateful for his contributions to popular culture, for the many decades of fun he has provided to us all, and we look forward to what he will bring us in the future.
Click here to view the PAC-MAN™ induction video!
2019: Batman
DC LOGO, BATMAN: TM & © DC. Jim Lee.
In July of 2019, Batman, DC's iconic Super Hero, was honored as the inaugural inductee into the Comic-Con Museum Character Hall of Fame.
As part of the induction ceremony, Jim Lee, DC Publisher and Chief Creative Officer, summed up why Batman is so deserving of the honor.
"Batman is very real. The core of Batman, the qualities that made him endure from generation to generation is as true to life as it gets. Batman is an inspiration that makes us believe that it's possible to take any hardship and forge it into something good. Batman is real in every person who summons the strength and resilience to keep going, and that my friends is a superpower."
Sign up for Museum Insider Newsletter!
Comic-Con Museum
Museum CHOF Sponsors 2020


The music room got some tweaking around the holidays when I redid the living room and is well on its way to being "done".
I posted about the wall art here.
I'd like to find two small round matching side tables and either table lamps or floor lamps to go along side the chair and a halves. I'm also considering an oversized leaning mirror against the wall on the left, partially behind the chair there.
And I'm trying out a cool new feature! If you want to shop the furniture, fixtures, and decor seen in my home, you can shop by clicking on them below!


We frequently receive questions from our members and clients about our current approach to testing. Some of you have accounts in our test system, some of you have the "demo" accounts introduced earlier this year, and all of you have access to the test prefix 10.5072 as part of your regular production account. What's the difference? Which one are you supposed to use for what?
These different options seem to have generated unnecessary confusion and therefore we're streamlining our approach to test accounts. We want to make a clear distinction between when you're doing something real and lasting vs. something that's just a test. This means that moving forward there will be production accounts (for real stuff) and test accounts (for fake stuff). That's it.
Your production account is an account in our production system. Everything you do there has the potential to be live. A Findable DOI resolves in the real world. Draft DOIs you make are still private, but they can be upgraded to Registered or Findable DOIs that are actually registered with the global handle server.
Your test account is an account in a completely separate test system. Nothing you do there is or has the potential to be live. You have access to all the same functions as in the production system, but everything you do is only pretend. A DOI marked Findable in the test system is not actually resolvable in the real world, but it will mimic that functionality so you can see what it looks like.
Every Provider will receive a test account. This will allow Providers to create test accounts for their Clients to use.
Demo accounts will be discontinued. If you currently have a demo account, DataCite staff will be contacting you individually throughout November to assign you a test account instead.
The test prefix 10.5072 will be discontinued. New users will not receive a test prefix as part of their production accounts during onboarding. For existing users, the test prefix will be officially removed from your production account in March 2019. This will give you time to adjust your workflows and request a test account to fulfill your testing needs.
We hope that these changes will ultimately make testing DataCite services easier to understand. For more information on test accounts, see our newly updated documentation at https://support.datacite.org/docs/testing-guide. And if you have any questions or concerns, please contact us at support@datacite.org.


A lot of white women these days are open to the idea of dating a black man which is a big contrast to 20 years ago where interracial relationships were not socially acceptable. When people saw an interracial couple in the street, they were often greeted with the disapproving look but things have changed now and society is more minded, however, in certain circumstances, most white women find themselves falling head over heels in love with a black man who is married. When this happens what should they do? Here are a few important tips for white women who are in love with a married black man.
It is very important for every woman out there black or white to think the exact same way a man would think if they want to be happy in their love life. There are not many men out who are ruining their lives because of the woman in their life because there is this idea that most men do not put their love life first especially if they are looking to succeed in life. This is part of the reason why most men look happier than women because love is blind and millions of women everywhere are willing to change locations, leave family and friends behind, drop out of school or even put future plans on hold for the sake of a man. This is the wrong way to go by things even if the guy is not married to anyone, however, if he happens to be married, this can be a kick in the teeth. It is a massive blow because as a woman in love, you are giving up a piece of yourself for a person who is always taken. Over time it will make you resentful which is never a good thing. Look at the man for what he is and if he is telling little white lies just to keep around, it is time to re-evaluate the interracial relationship you have.
If you can somehow better your lifestyle in the process like have the man pay for your bills, buy you a car or a home, fund your trips and buy you expensive gifts, when things go south and not working anymore, you will at least have something to show for the time spent together. It can be seen as cruel in the eyes of other people but if he is playing you along while he is married, that is a good way to get back at him.
You have to be really honest with yourself when dealing with a married man because if he is still married to his wife, he has no plans to leave her. Many men have the tendency of dating a woman until she starts to expect more from him then he leaves her for another. The cold hard truth is most guys will take a woman for a ride for as long as they allow him to hence why you have to be true to yourself so that you are not seen as doormat.


There is a lot of folklore about cider, from stories of rats in the press, to complicated definitions of scrumpy vs. cider, but the truth is its one of the oldest and more honest drinks - and on my first attempt - could not believe the simplicity involved in making it. Some will tell you the trick is in the right combination of apples - with a good mixture of sweet and sharp, and cider apples have a higher tannin content, giving extra bite etc, and there is some logic to this, but of course, unless you are taking this very seriously, you will be like me, offered a pile of apples and decide to have a go at making cider. My theory is - if the juice in its raw state is quite drinkable, you will probably make an ok cider out of it.
First you need to aquire your apple juice - either from apples - or I have heard of people making acceptable cider from shop apple juice (the kind without preservatives!). Assuming you are not going to cheat!, you need to wash and chop your apples, mash them somehow, then press them. You can hire a crusher and press from brewery shops for a reasonably rate, or you can buy a cheap pulper blade to put on your drill and make your own press . We have found that freezing the apples first does help extract lots of juice - but you have to have plenty of freezer space for this.
Fit an airlock and let the bubbling begin. It will look pretty muddy to start with, and a sediment will settle out. Rack after a few weeks, then leave to work itself out. When it has completely finished - bottle into plastic fizzy drinks bottles. Often the bottling process can cause a secondary fermination, and really that is a bad idea with glass bottles... if they burst. If you prefer, you can make your cider sparkling by either letting this secondary fermination take place, or add a small amount of sugar to each bottle (about 1/2 tsp per pint) and wait until the bottles feel tight, then move to a cool place.
The result? Clear, slighly acid but drinkable, fairly strong (in a sneak up on you sort of way!). Easy to do, easy to drink.


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Canon Europe and Canon Production Printing Announce Senior Executive Changes
6th Dec 2022 | Source: APO Group
Canon Europe (www.Canon-Europe.com/) today announced Taz Nakamasu, Executive Vice President Digital Printing & Solutions (DP&S) for Canon EMEA, has been appointed Global CEO Canon Production Printing (CPP). He replaces Mick Asada who will be returning to Tokyo to take on a new Canon Inc. headquarter assignment at the end of the year.
For the past four years, under Mick's leadership, CPP has expanded its portfolio and has focused on new growth areas like Graphic Arts
Mr Nakamasu has over four decades of experience with Canon where he has served in various senior leadership roles including Group Executive of OIP Small Office System Group, Canon Inc, Group Executive of Corporate Planning Development, Canon Inc, as well as President of Canon Australia.
For the past six years, Mr Nakamasu has held the position of Executive Vice President, Digital Printing & Solutions for EMEA where he has significantly contributed to the strategic direction and reformation of this business area and range of products, solutions and services. This included integrating the sales and marketing responsibility for Canon Production Printing's portfolio into EMEA bringing the technology even closer to its customers. He will take on this new role based in Venlo, the Netherlands from January 1, 2023.
Commenting on the appointment, Taz Nakamasu said "I am very excited for the opportunity to lead Canon Production Printing at such a critical time. For the past four years, under Mick's leadership, CPP has expanded its portfolio and has focused on new growth areas like Graphic Arts. I look forward to working with the team and continuing the business evolution."
Hiro Imamura, currently Vice President Marketing & Innovation EMEA Digital Printing & Solutions, will be promoted to Executive Vice President DP&S, Canon EMEA. Mr Imamura brings nearly 40 years of collective experience in the printing industry and as well as previously working in Canon Inc. and Canon USA sales region. He will commence in this new role from January 2023. A successor will be announced shortly.
Distributed by APO Group on behalf of Canon Central and North Africa (CCNA).
Partech raises fourth seed fund
The extremely unsecure crystal ball


Remember back to some of your life's most memorable moments? Maybe it was a graduation, sports event, or wedding? Or perhaps it was a birthday, anniversary, baby shower, or funeral? Can you imagine if there was no music at these events? For many of us, music is the tapestry that paints the picture of our life's memories. Learning to sing, can help you be even more connected!
My Local Music Lessons offers Voice Lessons in Aurora CO. More and more busy families and professionals are enjoying the benefits of in home music programs. We save you time and give you peace of mind! Also, we have a convenient online schedule system. First, enter your location and instrument. Next, choose your instructor and a time and day to meet. Also enter coupon code 1stfree at checkout for 25% off your 1st 4 pack. The top performers in the world have voice coaches and all study voice lessons. Vocal training is important for anyone who wishes to pursue a career in singing or acting. We provide professional training, and make singing FUN! If you are looking for Voice Lessons Aurora CO, you found the right place.
Human anatomy and physiology as it relates to the physical process of singing.
First, Music Theory is the foundation that all music is created with. If you are a scientist, you had to pass Chemistry 101. Music Theory is the science of music and explains everything in the music world. This includes intervals, ear training, keys and key signatures. Also, it includes chords in a key, and variations to those chords. In addition, we learn about variations include color chords, connector chords, and substitute chords. Finally, we teach you how these variations affect the melody, which is what the singer sings!
Are you struggling with fear of performance or public speaking? Voice lessons can help you overcome this fear. First, you will build confidence with our step by step programs! Gaining a foundation you can count on will build confidence. Subsequently, we will help you become free of the fear of failure. Also, studies show singing has health benefits. In addition, singing boosts brain development and can increase IQ. Subsequently singing teaches discipline. When you learn to sing your favorite songs, you will get satisfaction! Finally, singing is FUN!
Use your diaphragm! Correct breathing techniques in voice lessons are the most important skill to learn. First, proper singing techniques involve your whole body. Relying solely on your vocal chords to hit notes in a song will cause pain and strain. Also, if you feel any pain you are not using proper technique. In fact you are potentially causing damage. In order to sing using your diaphragm you need to have good posture. Your singing teacher will work with you to plan your breaths accordingly. Finally, you will have enough air to hit the notes and to finish the note without cutting it short or trailing off.
First, click the "purchase lessons" button. Next enter your location and instrument. Also, choose an instructor as well as a time and day. Finally, enter coupon code 1stfree at checkout for 25% OFF your 1st 4 pack! We save you time and give you peace of mind! Email booking@mylocalmusiclessons.com or call 303 558 6160 with questions. See our website My Local Music Lessons, our Instructors Page, and our Helpful Info Page. Email us at booking@mylocalmusiclessons.com or call 303 558 6160 with questions!


An unofficial theme was set for the MATS Bootcamp for April as underwater themed and dishware.
I'm not sure I like how uniform the symmetry is as the original sketch was much more organic – think I might try redrawing it.
sea anemone repeat pattern in black/white and green bg.
band of colour around the edges with anemone motifs in circular formation – the ones underneath are the tentacles radiating from the centre.
sea anemone on 'traditional' style plate layout.
These are not quite what I envisaged when I started … I'm not sure if thats a good thing – I need a rethink.
here's another plate mockup set – I just added my Sea Anemone to some plates I found on the internet (fab plates!!) sea coloured one on Anthropologie website and white dinner plate is Portobello-porcelain from The White Company – tried varying the size of the graphic as well – I really want to re-draw this whole thing as I preferred the original sketch where it was far more organic and something very liquid about the 'mouth' part – this doesn't seem very 'mouth' like at all… the mouth of a Sea Anemone.
update: actually I decided to make a more complicated repeat… I've made several and now I have a sudden urge to poke my eyes out as I've been up all night! gargh.
I think it's too small – ok… I'll postpone poking my eyes out (it's a shape you can't swop your eyes out for a bit!) and see if I can change it.
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