Seven Ehrlichia strains (six HF strains and one Anan strain) that were obtained from laboratory mice by intraperitoneally inoculating homogenates of adult Ixodes ovatus collected in Japan were characterized. 16S rRNA sequences of all six HF strains were identical, and the sequences were 99.7, 98.2, and 97.7% identical to those of Anan strain, Ehrlichia chaffeensis (human monocytic ehrlichiosis agent), and E. muris, respectively. Partial GroEL amino acid sequencing also revealed that the six HF strains had identical sequences, which were 99.0, 98.5, and 97.3% identical to those of E. chaffeensis, the Anan strain, andE. canis, respectively. All HF strains were lethal to mice at higher dosages and intraperitoneal inoculation, whereas the Anan orE. muris strain induced only mild clinical signs. Light and electron microscopy of moribund mice inoculated with one of the HF strains revealed severe liver necrosis and the presence of numerous ehrlichial inclusions (morulae) in various organs. The study revealed that members of E. canis genogroup are naturally present inIxodes ticks. HF strains that can cause severe illness in immunocompetent laboratory mice would be valuable in studying the pathogenesis and the roles of both cellular and humoral immune responses in ehrlichiosis caused by E. canisgenogroup.
Ehrlichiae are obligatory intracellular bacteria that infect monocytes/macrophages, granulocytes, or platelets and cause a noncontagious, febrile systemic illness called ehrlichiosis in humans and in some varieties of domestic and wild animals (23-26). The severity of the disease varies from asymptomatic seroconversion to death, and severe morbidity is frequently documented. Ehrlichioses are now known as important emerging vector-borne zoonoses in the United States (1, 2, 4, 5, 23-26,33). Five different species of Ehrlichia (E. chaffeensis, human granulocytic ehrlichiosis [HGE] agent,E. sennetsu, Venezuelan human Ehrlichia [VHE, a strain of E. canis], and E. ewingii) are now known to infect humans. Sennetsu fever in western Japan and in Malaysia is caused by E. sennetsu (8, 17, 34). Human monocytic ehrlichiosis (HME) in the United States is caused by E. chaffeensis (1), and asymptomatic human infection with VHE occurs in South America (21). HME in Europe and Africa (11, 18, 32) is probably caused by anEhrlichia sp. closely related to E. chaffeensis. HGE in the United States and Europe is caused by an ehrlichial organism called HGE agent, which is closely related to E. equi andE. phagocytophila (5, 10). HGE caused by infection with another granulocytic Ehrlichia sp., E. ewingii (canine granulocytic Ehrlichia), was recognized in Missouri last year (4).
Ehrlichia spp. are transmitted to humans by specific species of infected ticks or trematodes from specific species of infected wild-animal reservoirs. For example, E. chaffeensis has been most commonly identified in the Lone Star tick (Amblyomma americanum) (3), and white-tailed deer are considered to be the major reservoir of E. chaffeensis (6,15). The HGE agent has been found in the deer tick (Ixodes scapularis) (20, 31), and white-footed mice are considered to be the major reservoir of the HGE agent in northeastern and midwestern United States (31). Most Ehrlichiaspp. characterized so far were isolated from domestic animals or humans. Only a few Ehrlichia spp. have been isolated from wild animals or vectors. Examination of Ehrlichia spp. in vectors and wild animals would provide an understanding of natural distribution and maintenance of ehrlichial organisms, as well as the diversity and evolution of ehrlichial populations. Such a study would provide a risk assessment for acquiring ehrlichial infection in particular geographic regions and, therefore, would facilitate proactive preventive measures. In 1983, Kawahara et al. isolated an infectious agent inducing splenomegaly in laboratory mice from a wild mouse (Eothenomys kageus) caught in central Japan. This organism was shown to be closely related to Ehrlichia canisby morphological and antigenic analysis (12), and 16S rRNA gene sequencing revealed it to be most closely related to E. chaffeensis. Since it is sufficiently distinct from any knownEhrlichia spp., it was designated E. muris(35). E. muris has been isolated from two additional species of wild mice in Metropolitan Tokyo and fromHaemaphysalis flava ticks in Japan (14) (Fig.1). Seroepidemiologic data suggested the exposure of humans and various wild animals to E. muris or related species in Japan (14).
Geographic region where Ehrlichia spp. were isolated from ticks or wild mice. Tick isolates are boxed. An asterisk indicates an isolate used for this study. Letters designate sources as follows: a, Fujita and Watanabe (7); b, Kawahara et al. (12); and c, Kawahara et al. (14).
Fujita and Watanabe (7) isolated 14Ehrlichia-like agents from Ixodes ovatus ticks collected in two northern prefectures in Japan from 1983 to 1994 (Fig.1). In the present study, we characterized five isolates collected from 1993 to 1994, which were reported by Fujita and Watanabe (7), and two additional tick isolates that have not been reported previously. To accomplish this analysis, we examined genetic, antigenic, and ultrastructural features and concluded that the strains are most closely related to E. chaffeensis, followed by E. muris and E. canis. Histopathologic observations in infected immunocompetent mice suggests that these new strains would be valuable in studying disease mechanisms and the role of immune responses in ehrlichiosis caused by the E. canisgenogroup.
Ehrlichiae isolated from I. ovatusticks.Five isolates (HF565, HF568-1, HF568-2, HF639-2, and HF642) were reported previously (7), and two isolates (HF652 and Anan) were obtained in the present study. HF652 was isolated fromI. ovatus collected in Aomori Prefecture in 1994, and Anan was isolated from I. ovatus collected in Tokushima Prefecture in 1994 (Fig. 1). All ticks were collected from vegetation with a standard 1-m2 flannel flag. Isolation ofEhrlichia spp. was done as described elsewhere (7). Briefly, ticks were soaked for 10 min in 70% ethanol with 0.1% povidone-iodine and then rinsed with phosphate-buffered saline (PBS, pH 7.2) with 0.5 to 1.0% calf serum (GIBCO, Grand Island, N. Y.). Of the pooled ticks, two to six—either male or female—were ground up with a depression slide glass and a glass pestle (Iuchi Co., Ltd., Osaka, Japan) or with a mortar and pestle in sucrose-phosphate-glutamate (0.0038 M KH2PO4–0.0072 M K2HPO4–0.0049 M l-glutamate–0.218 M sucrose [pH 7.2]) at 0.3 ml per tick. For isolation of infectious agents, 0.3 ml of the homogenate of each pool was inoculated intraperitoneally into one 6-week-old female ddY mouse (Funabashi Farm, Shizuoka, Japan). These isolates had been passaged through mice once or twice.
Analysis of 16S rRNA and GroEL sequences of strains.DNA was extracted from the spleens harvested from mice infected with strains HF565, HF568-1, HF568-2, HF639-2, HF642, HF652, or Anan by using a blood kit (Qiagen, Inc., Valencia, Calif.). PCR amplifications and nucleotide sequencing of total 16S rRNA genes were performed as previously described (14).
PCR amplification and nucleotide sequencing of groEL genes in DNA from the spleens of mice infected with HF and Anan isolates were performed by the method of Sumner et al. (30). The primer pairs used were HS43 and HS45 (30), Cha792u (5′-GGTGATGGAACAACTACATG-3′) and Cha1429d (5′-CCWARCATRTCTTTTCTTCT-3′), and Cha1293u (5′-GARGTDDARGGTGAAGC-3′) and Cha1770d (5′-TTCAACAGCAGCTCTAGTTG-3′), which were designed based onE. chaffeensis and HS43-HS45-amplified products. The PCR products were sequenced by using the PCR primers.
Phylogenetic analysis of DNA and amino acid sequences.Sequence data were prepared for analysis by using the AutoAssembler version 1.4 (Perkin-Elmer, Norwalk, Conn.). The corrected levels of divergences of nucleotide of the 16S rRNA gene and deduced amino acid sequences of GroEL were calculated by using the Genetic Information Processing Software GENETYX-WIN version 3.0 (Software Development Co., Tokyo, Japan) on a Power Mac 8500. A dendrogram was constructed by using the UPGMA method and data from the distance matrix.
Clinical signs, relative spleen size, and indirect fluorescence antibody (IFA) titer against E. muris.The 10% homogenates of spleens prepared from each mouse infected with one of the six HF strains at days 9 to 10 postinfection (p.i.), just before death, at a 1:10 dilution and the 10% homogenate prepared from the mouse infected with the Anan strain at day 10 or 15 p.i. without dilution were inoculated intraperitoneally or subcutaneously at 0.2 ml/mouse into five BALB/c male mice (8 weeks old). As positive controls the homogenates from mice infected with three strains of E. muris AS145 (type strain ), I-268 (14), and NA-1 (14) at 10 days p.i. were inoculated into five mice each. The spleen homogenates used as inocula contained similar levels of ehrlichial DNA based on the band intensity of the PCR products, except for that of the Anan isolate. The band intensity of the Anan isolate in the 10% spleen homogenate corresponded to an ∼103 dilution of isolates from HF strains. To assess the ehrlichial amount in the inoculum, the PCR amplification of each of the strains was performed by using a thermal cycler (Sankojyunyaku Co., Ltd., Tokyo, Japan) and GeneAmp reagents (Takara, Kyoto, Japan). A pair of primers, EC9 (5′-AAGGATCCTACCTTGTTACGACTT-3′) and EC10 (5′-AATCTAGATTAGATACCCTDGTAGTCC-3′, in which D = A, T, or G) were used based on the bacterial sequence of 16S rRNA (1). Each sample was amplified for 3 cycles at 94°C (1 min), 48°C (2 min), and 66°C (1 min 30 s), followed by 94°C for 9 min and 25 cycles at 88°C (1 min), 52°C (2 min), and 68°C (1 min 30 s). Products were electrophoresed through a 1.5% agarose gel to assess amplification efficiency. A 733-bp fragment was obtained after the amplification of DNA templates from all isolates.
The clinical signs of inoculated mice were observed daily until death or ≤20 days p.i. The relative spleen indexes (in grams per 100 g of body weight) were compared among all mice inoculated intraperitoneally on days 8 and 10 p.i. Antibody titers were compared for all mice inoculated subcutaneously on day 20 p.i. Antibody titers against E. muris, the HGE agent, andE. sennetsu were measured by the IFA test (12,29). Various tissues, including the liver, spleen, thymus, and bone marrow, were collected at days 7 to 10 from mice inoculated intraperitoneally with HF565 or Anan strain, fixed in 10% buffered formalin solution (pH 7.4), and embedded in paraffin. Sections were stained with hematoxylin-eosin and Giemsa.
Electron microscopy.The spleen and liver samples from mice intraperitoneally inoculated with strain HF565 at days 7 and 9 p.i. or with Anan strain at day 10 p.i. were cut into 1-mm-thick cubes and fixed and processed as described elsewhere (12). Ultrathin sections were stained with uranyl acetate and lead citrate, and the stained sections were examined with a Philips model 300 electron microscope at 60 kV.
Nucleotide sequence accession numbers.The GenBank nucleotide sequence accession numbers for the 16S rRNAs used for comparison in this study are as follows: E. chaffeensis,M73222; E. canis, M73221; E. ewingii, M73227;E. sennetsu, M73225; E. muris, type strain AS145,U15527; strain I-268, AB013008; and strain NA-1, AB013009. The nucleotide sequences of strain HF565 and strain Anan have been deposited in the GenBank data library under accession numbersAB024928 and AB028319, respectively. The accession numbers ofgroEL sequences used for sequence comparisons are Z15160 forBartonella bacilliformis, U13638 for Cowdria ruminantium, L10917 for E. chaffeensis, U88092 forE. sennetsu, X07850 for Escherichia coli, U96728for HGE agent, U96727 for E. equi, U96731 for E. canis, and U96729 for E. phagocytophila. Nucleotide sequences of strain HF-565 and strain Anan have been deposited in the GenBank data library under accession numbers AB032712 and AB032711, respectively.
16S rRNA gene and partial GroEL amino acid sequences and phylogenetic analysis.All ehrlichial strains isolated from wild mice or ticks in Japan, including three strains of E. murisAS145 (type strain, 11), I-268 isolated from an Apodemus speciosus wild mouse in Metropolitan Tokyo (14), or NA-1 isolated from a H. flava tick in Aichi Prefecture (14), are shown in Fig. 1. A total of 16 strains were isolated from adult I. ovatus: 12 strains in Fukushima Prefecture from 1983 to 1994, 3 strains in Aomori Prefecture from 1984 and 1994, and 1 strain in Tokushima Prefecture in 1994 (Fig. 1) (7). Four Fukushima strains (HF565, HF568-1, HF568-2, and HF639-2), two Aomori strains (HF642 and HF652), and one Tokushima strain (Anan) were examined in the present study.
Of the 1,449-bp 16S rRNA gene sequences compared, all HF strains had identical base sequences and between Anan and HF strains, only three bases were different (Table 1). Anan and HF strains belonged to the E. canis genogroup and were far apart from the E. phagocytophila (the HGE agent) andE. sennetsu genogroups (Table 1 and Fig.2). The HF strains were closest toE. chaffeensis, even more so than E. muris: HF strains and E. muris differ from E. chaffeensisby 19 and 29 bases, respectively (Table 1).
Phylogenetic relationships between Anan, HF, and otherEhrlichia strains based on 16S rRNA gene sequence comparisons. HF strains included HF565, HF568-1, HF568-2, HF639-2, HF642, and HF652.
Of the 409 deduced amino acid sequences of GroEL compared, all six HF strains had the identical amino acid sequence (Table2). All strains belonged to E. canis genogroup and were far from the E. phagocytophila(the HGE agent) or E. sennetsu genogroup (Table 2 and Fig.3). HF strains were closest to E. chaffeensis, followed by the Anan strain and E. canisin the dendrogram (Fig. 3). Between HF strains and E. chaffeensis, four amino acids were different, and between HF and Anan strains six amino acids were different (Table 2).
Phylogenetic relationships generated with UPGMA based on an alignment of the first 409 amino acid sequences of GroEL of HF and Anan strains and other members of the tribe Ehrlichieae.
Antibody reactivity.Antibody titers against E. muris were examined by the IFA test in the sera collected on day 20 p.i. from mice inoculated subcutaneously. The titers of sera from mice inoculated with all I. ovatus isolates were comparable with three strains of E. muris: AS145, I-268, and NA-1 (Table 3). There was no cross-reactivity of these sera with E. sennetsu or the HGE agent. Because E. muris, E. chaffeensis, E. canis, VHE, and E. ewingii are antigenically highly cross-reactive to each other (1, 2, 4, 12, 14, 21, 23-26), this result is in agreement with the 16S rRNA gene and GroEL sequencing data showing that all I. ovatus isolates examined belong to the E. canis genogroup rather than the E. phagocytophila or E. sennetsu genogroup.
Mouse pathogenicity.When ca. 10% spleen of the homogenate of mice containing each strain was intraperitoneally inoculated, starting ca. day 7 p.i., all mice inoculated with six HF strains developed clinical signs of ruffled fur, inactivity, anorexia, dehydration, and weight loss, and they died from days 8 through 10 p.i. (Table 3). The lethality of the HF strains was dependent on the dosage and route of inoculation. With a dilution of up to 102 of the inoculum, all mice inoculated intraperitoneally died at days 8 to 10 p.i., but at a 104 dilution all mice survived. With the subcutaneous route, two of five mice died on days 8 to 10 p.i., but with >10-fold dilutions of inoculum all mice survived for more than 20 days p.i., at which time the experiment was terminated. The mice inoculated intraperitoneally with the Anan strain without dilution developed mild clinical signs similar to those inoculated with the E. muris strains (12, 13). Mice inoculated subcutaneously with the Anan isolate did not develop any significant clinical signs. None of mice inoculated intraperitoneally with the three strains of E. muris AS145, I-268, or NA-1 died during the 20-day p.i. period (Table 3). By PCR, ehrlichial DNAs present in 10% of the spleen homogenates were similar among all strains, including E. muris strains, except the Anan strain. The Anan strain in the spleen was approximately 1/103 of the HF565 strain in the spleen. Lack of death with the Anan strain was, however, not caused by small amounts of organisms in the inoculum. When the Anan strain at the same DNA level as that of the HF565 strain was intraperitoneally inoculated into mice, it did not kill them (data not shown).
At necropsy, mice inoculated with all strains developed splenomegaly. When spleen sizes were compared among intraperitoneally inoculated mice on days 8 to 10 p.i., the degrees of splenomegaly were greater in the following order: I-268, NA-1, E. muris (AS145) > Anan > all HF strains (Table 3). The mean relative spleen size of uninfected mice was 0.42 ± 0.04 (n = 5); therefore, there were seven- to twofold increases in the spleen size. The spleens were dull dark red and tender in HF strain-inoculated mice. In contrast, the tissues were less dark and firm in mice inoculated with Anan or E. muris strains.
Light and electron microscopy of organs of infected mice.By light microscopy of paraffin sections numerous dark-blue (Giemsa)- or purple (hematoxylin-eosin)-stained cocci in round inclusions (morulae) or by light microscopy of Epon-embedded 1-μm sections light-blue (toluidine blue)-stained organisms could be visualized. These organisms were frequently observed in the cytoplasms of mononuclear cells in the lumen and along the wall of small blood vessels in the spleen, liver, thymus, lung, bone marrow, and large intestine of mice intraperitoneally inoculated with strain HF565 on days 7 and 9 p.i. (Fig. 4). Prominent neutrophilic inflammatory cell infiltration was not evident in any of these organs. In the spleen, the red pulp was expanded, the white pulp was disorganized, and the follicles were not significantly activated. Thymic severe lymphoid depletion as seen in E. risticii-infected mice (27) was not evident in the mice infected with HF strain. The liver showed severe necrosis, especially around the central veins. Morulae were not seen in the necrotic area. In contrast, morulae were not detectable in the spleen of Anan strain-infected mice on days 10 and 15 p.i. The spleens of mice inoculated with Anan strain had increased cellularlity and well-developed follicles even at day 7 p.i. (data not shown).
Light micrographs of paraffin-embedded section of the thymus (A) and the lung (B) from the mice intraperitoneally inoculated with HF565 strain at day 9 p.i. Note several morulae packed with many ehrlichial organisms (colonies of ehrlichiae) along the capillary (arrows) (A) [magnification, ×1,070]) and in the capillary of the alveolar wall (arrows) (B) [magnification, ×1,700]). Hemtoxylin and eosin staining was used.
Under an electron microscope, numerous ehrlichial organisms were detected in membrane-bound inclusions in the cytoplasm of monocytes, macrophages, and eosinophils but not in neutrophils in the red pulp of the spleen or in Kupffer cells in the liver from mice inoculated with strain HF565 (Fig. 5). The organisms were generally round, but some were pleomorphic and variable in size, ranging from 0.4 to 1.0 μm in length and 0.2 to 0.7 μm in diameter. Each organism was bound by two membranes, and the outer membranes were ruffled (Fig. 5). Significant amounts of ribosomes and a fine meshwork of DNA strands were evident (Fig. 5). As noted previously in E. canis inclusions in DH82 cells (23) and in various E. chaffeensis strains (25), the intramorular matrix contained tiny fibrillar materials in some morulae (Fig. 5). Intramorular tubules of approximately 25 nm in diameter, which are found in the inclusions of several strains of E. chaffeensis in DH82 cells (25), were occasionally seen (one is indicated Fig.5B). As noted in E. chaffeensis (25), occasional association of mitochondria with ehrlichial inclusions was seen (Fig.5A and B). These findings are in agreement with the 16S rRNA andgroEL gene sequencing data showing that HF565 strain belongs to E. canis genogroup. Ehrlichial organisms were not detectable in the spleens of Anan strain-infected mice by electron microscopy.
Electron micrographs of morulae of HF565 in the cytoplasm of a monocyte (A) and an eosinophil (B) in the blood vessel in the spleen and in a Kupffer cell in the liver (C) from the mouse at day 7 p.i. Note the numerous pleomorphic coccobacilli enveloped in two layers of membranes embedded in a fine filamentous matrix in the membrane-bound inclusion in panel A. An intramorular tubule is indicated by the arrow in panel B. The inclusions are tightly packed with ehrlichiae without intramorular space in the Kupffer cells adhered to the endothelial layer lining the sinusoid. Magnifications: ×24,200 (A), ×21,000 (B), and ×16,400 (C).
16S rRNA genes and GroEL amino acid sequences, antigenic cross-reactivities, and ultrastructural features indicate that ourI. ovatus isolates in Japan belong to the E. canis genogroup and are most closely related to E. chaffeensis. E. chaffeensis has been found so far primarily in Amblyomma americanum and, less frequently, inDermacentor variabilis in the United States (3). A small number of the I. scapularis ticks tested were negative for E. chaffeensis (3). MostEhrlichia spp. found in Ixodes spp. in the United States and Europe are Ehrlichia spp. belonging to E. phagocytophila, E. equi, and the HGE agent genogroup (24). However, a partial 16S rRNA gene sequence identical to that of E. muris was recently found in Ixodes persulcatus ticks in Perm, Russia (22). Therefore,Ixodes spp. can be naturally infected by two distinct ehrlichial genogroups. I. ovatus is a common tick found throughout Japan (38). The adult-stage tick bites humans and large animals, and the nymphal- and larval-stages tick bite small mammals such as rodents (38). Whether, like E. muris, wild mice are reservoirs of these I. ovatusisolates is yet unknown. Two species of ticks have so far been found infected with Ehrlichia spp. in Japan. We previously isolated E. muris from H. flava ticks (14). According to Fujita and Watanabe (7), the ehrlichial infection rate for I. ovatus (16 of 439 ticks examined) is higher than for H. flava (0 of 351 ticks examined).
By electron microscopy HF565 organisms, which belong to the monocytotropic ehrlichiae, were detected in eosinophils. It is unlikely that I. ovatus was coinfected with granulocytotropicEhrlichia sp., since mouse neutrophils were not infected and our serologic and gene sequencing data did not reveal any contamination of granulocytotropic Ehrlichia sp. in the inoculum. Ehrlichial organisms were previously seen in neutrophils in the blood of HME patients (16, 19). Therefore, although this is a rare event and monocytes are the primary hosts, in severe infections some granulocytes are infected with the E. canis genogroup. Whether this occurs with granulocytes from uninfected healthy hosts in vitro or due to alterations that occur in granulocytes in infected hosts is unknown.
Ehrlichia spp. can be subdivided into three genetically distinct groups (genogroups). Two ehrlichial genogroups have been isolated in Japan to date. E. sennetsu is the first ehrlichial organism discovered in Japan. It was isolated from the blood of patients with "Hyuga fever" or "Kagami fever," which was endemic in Kyushu, Southern Japan, in the 1950s (8, 17). SF agent, which belongs to E. sennetsu genogroup (36), had originally been isolated in Japan in 1962 fromStellantchasmus falcatus metacercarial parasites on gray mullet fish in Kyushu (9). The second genogroup organisms isolated were the E. muris strains (12,14) and I. ovatus strains described here. The thirdE. phagocytophila genogroup has not yet been identified in Japan.
HF strains, when inoculated intraperitoneally, were more virulent in immunocompetent laboratory mice than any other ehrlichial strains we examined. Because all six strains obtained at different times in different geographic regions had similar levels of virulence, it is unlikely that the viruses or other agents are contaminated in all of these isolates. We have tried to cultivate HF and Anan strains by using various cell lines. We have not cultivated any of them yet, but we have not found any contamination by bacteria, viruses, or parasites in these isolates. Haemobartonella or Eperythrozoon spp. were not detectable on a blood smear or by a PCR based on the 16S rRNA gene sequences we determined (28). Therefore, it is unlikely that other contaminating agents are responsible for the virulence of the HF strains.
Of significance is the striking difference in mouse pathogenicity between HF strains and the Anan strain, despite the closeness of their 16S rRNA gene sequences. Massive ehrlichial proliferation was evident in immunocompetent mice with HF stains, whereas proliferation of the Anan strain-like E. muris appears to be held in check. As previously noted with E. muris infection of mice (13), this may be due to active immune stimulation with the Anan strain, as evidenced by increased cellularlity and follicle stimulation in the spleen. On the other hand, with lethal dosages of HF strain, the spleen structure was disorganized and follicle stimulation was not evident. With the HF strain widespread prominent liver necrosis similar to that previously noted in fatal HME (D. H. Walker, J. P. Taylor, J. S. Blie, and C. Dearden, Abstr. 89th Annu. Meet. Am. Soc. Microbiol. 1989, abstr. D76, p. 95, 1989) or in SCID/beige mice experimentally infected with E. chaffeensis(37) was seen. In connection with this observation, one of the characteristic laboratory findings of HME as well as with HGE is increased liver enzyme activities. What gene products of HF strain are responsible for this fulminant infection in immunocompetent mice is unknown. These two isolates, i.e., HF and Anan, the closest relatives of E. chaffeensis, may provide a valuable comparative immunocompetent mouse disease model for understanding pathogenesis and roles of immune responses in ehrlichiosis caused by the E. canis genogroup.
We thank Fumihiko Mahara for kindly providing accommodations for tick collection in Anan City, Tokushima, Japan.
A part of the study was supported by the grant RO1 AI30010 from the National Institutes of Health.
Returned for modification 8 December 1999.
(1991) Ehrlichia chaffeensis, a new species associated with human ehrlichiosis. J. Clin. Microbiol. 29:2838–2842.
(1992) Ehrlichia ewingii sp. nov., the etiologic agent of canine granulocytic ehrlichiosis. Int. J. Syst. Bacteriol. 42:299–302.
(1993) Amblyomma americanum: a potential vector of human ehrlichiosis. Am. J. Trop. Med. Hyg. 49:239–244.
(1999) Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. New Engl. J. Med. 341:148–155.
(1994) Identification of granulocytic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32:585–595.
(1995) Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Ambryomma americanum (Acari: Ixodidae). J. Med. Entomol. 32:368–374.
(1996) Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N. Engl. J. Med. 334:209–215.
(1991) Human infection by Ehrlichia canis in Spain. Med. Clin. 96:236–237.
(1993) Characterization of ehrlichial organisms isolated from a wild mouse. J. Clin. Microbiol. 31:89–96.
(1996) Impaired antigen-specific responses and enhanced polyclonal stimulation in mice infected with Ehrlichia muris. Microbiol. Immunol. 40:575–581.
(1999) Comparison of Ehrlichia muris strains isolated from wild mice and ticks and serologic survey of humans and animals with E. muris as antigen. J. Clin. Microbiol. 37:1123–1129.
(1997) Isolation of Ehrlichia chaffeensis from wild white-tailed deer (Odocoileus virginianus) confirms their role as natural reservoir hosts. J. Clin. Microbiol. 35:1681–1686.
(1987) Human infection with Ehrlichia canis, a leukocytic rickettsia. N. Engl. J. Med. 316:856–853.
(1991) First European cases of ehrlichiosis. Lancet 338:633–634.
(1997) Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. J. Clin. Microbiol. 35:2496–2502.
(1995) Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J. Infect. Dis. 172:1007–1012.
(1996) Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J. Clin. Microbiol. 34:2133–2139.
(1999) Monocytic Ehrlichia in Ixodes persulcatus ticks from Perm, Russia. Lancet 353:722–723.
(1991) The tribe Ehrlichieae and ehrlichial diseases. Clin. Microbiol. Rev. 4:286–308.
(1996) Ehrlichiae. in Proceedings of the 5th International Symposium on Rickettsiae and Rickettsial Diseases Slovak Academy of Sciences, Bratislava, Slovak Republic. eds Kazar J., Tomen R. (International Society of Rickettsiae and Rickettsial Diseases, Bratislava, Slovak Republic), pp 272–286.
(1999) Clinical and biological aspects of infections caused by Ehrlichia chaffeensis. Microb. Infect. 3:1–10.
(1999) Ehrlichiae of veterinary importance. in Rickettsiae and rickettsial diseases at the turn of the third millenium. eds Raoult D., Brouqui P. (Elsevier, Paris, France), pp 393–405.
(1987) Reduced immune responsiveness and lymphoid depletion in mice infected with Ehrlichia risticii. Infect. Immun. 55:2215–2222.
(1997) Western immunoblot analysis of Haemobartonella muris and comparison of 16S rDNA sequences of H. muris. H. felis, and Eperythrozoon suis. J. Clin. Microbiol. 35:823–829.
(1997) Direct isolation and cultivation of human granulocytic ehrlichia from a human patient. J. Infect. Dis. 175:210–213.
(1996) Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc. Natl. Acad. Sci. USA 93:6209–6214.
(1992) A case of human ehrlichiosis acquired in Mali: clinical and laboratory findings. Am. J. Trop. Med. Hyg. 46:161–164.
(1990) Biological properties of the genus Ehrlichia: substrate utilization and energy metabolism. in Ehrlichiosis: a vector-borne disease of animals and humans. eds Williams J. C., Kakoma I. (Kluwer Academic Publishers, Boston, Mass), pp 59–67.
(1995) Ehrlichia muris sp. nov., a new species of Ehrlichia, identified on the basis of 16S rRNA base sequence, serological, morphological, and biological characteristics. Int. J. Syst. Bacteriol. 45:250–254.
(1996) Characterization of SF agent, an Ehrlichia sp. isolated from the fluke Stellantchasmus falcatus, by 16S rRNA base sequence, serological, and morphological analyses. Int. J. Syst. Bacteriol. 46:149–154.
(1998) Infection of the laboratory mouse with the intracellular pathogen Ehrlichia chaffensis. Infect. Immun. 66:3892–3899.
(1980) Ixodes ovatus Neumann. in Illustrations of the mites and ticks of Japan. Zenkoku Nosonkyoika Kyokai, Japan. (In Japanese.), ed Ehara S. pp 149–155.


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Wonderwall To Be Here
The Adventures of Ghosthorse and Stillborn (Touch and Go, 2007) is the third full length studio album by CocoRosie. It was produced by Valgeir Sigurðsson, Björk's longtime collaborator. CocoRosie is a musical group formed in 2003 by sisters Bianca "Coco" and Sierra "Rosie" Casady. The sisters were born and raised in the United States, but formed the band in Paris after meeting for the first time in years. As children, the sisters spent summers with their father, an Iowa farmer who became interested in Native American religion, while he visited Indian reservations and took part in vision quests. The album's cover is a photo by Pierre et Gilles that features Sierra, Bianca, and Bianca in drag. Their music has been called "freak folk", and incorporates elements of pop,blues, opera, electronica, and hip hop. On May 13, 2008, CocoRosie released a new single entitled "God Has a Voice, She Speaks Through Me". The nomadic duo (currently and temporarily residing in New Mexico) spent much of 2008 writing and recording in Buenos Aires, Melbourne, Berlin, New York, and Paris, finding amazing and diverse musicians to collaborate along the way. The result is their 11-song 2010 Sub Pop debut, Grey Oceans. CocoRosie was named the 16th most influential artist of the decade by betterPropaganda in 2009.

redpajama_set_name: RedPajamaCommonCrawl


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Guillaume Deffontaines, né en , est un directeur de la photographie français.

Biographie

Guillaume Deffontaines fait ses études à l'École nationale supérieure Louis-Lumière. Il commence sa carrière professionnelle comme assistant caméra (auprès de Christophe Beaucarne), puis assistant photographie (notamment sur Paris de Cédric Klapisch), avant de devenir directeur de la photographie. Il collabore depuis régulièrement avec Bruno Dumont.

Filmographie

Distinction

Nomination
 2017 : César de la meilleure photographie pour Ma Loute

Notes et références

Liens externes

 Guillaume Deffontaines à l'Afc

Directeur de la photographie français
Étudiant de l'École nationale supérieure Louis-Lumière
Naissance en 1968

redpajama_set_name: RedPajamaWikipedia


Diagnostic imaging equipment is used by medical practitioners to study the human body and its complex structure. By using imaging technologies, monitoring, diagnosing, screening, and treating a wide range of medical ailments is made easier and more efficient. The improved efficiency of these devices has helped the healthcare industry in delivering effective medical intervention, as it has remarkably improved the ability of doctors to treat and diagnose any injury or disease.
In the past few years, the global diagnostic imaging devices market has witnessed steady growth due to the increasing number of geriatrics, high prevalence of chronic diseases and injuries, growing awareness about benefits of timely diagnosis, technological advancements, growing applications of diagnostic imaging devices, and improving investments. These factors are expected to drive the global diagnostics devices market to a figure of US$35.8 bn by the end of 2020 from US$26.4 bn in 2014, registering a CAGR of 5.2% during the forecast period therein. However, the global diagnostic imaging devices market faces certain restraints such as a high risk of cancer due to excessive exposure to radiation used in some diagnostic imaging devices, poor supply of helium for magnetic resonance imaging systems, and tough regulatory requirements.
The global diagnostic imaging device market is segmented on the basis of device type and geography. The types of devices used in this market are x-ray devices, magnetic resonance imaging (MRI), ultrasound systems, nuclear imaging systems, computed tomography (CT) scanners, and others. Ultrasound systems and x-ray diagnostic imaging devices are further divided on the basis of portability and technology. Furthermore, the magnetic resonance imaging devices segment is divided on the basis of architecture, while computed tomography scanners are sub-segmented on the basis of the type of slice. Geographically, this market is segmented into Europe, Asia Pacific, North America, and Rest of the World.
According to a research report, North America holds a dominant share in the global market for diagnostic imaging devices. This share of the regional market is attributable to the high prevalence of injuries and diseases in the region, along with an increasing pool of geriatric population.
Analysts predict, demand from Asia Pacific will boost the diagnostic imaging devices market, as the demand for healthcare increases in the developing nations of India and China. This positive trend is expected to continue in the coming five years. Other developing nations will also propel the diagnostic imaging devices market due to the huge pool of patients, growing awareness about diseases, increasing healthcare infrastructure, and improving government investments in the regions.
Ultrasound devices are popularly used for imaging, as they are the most effective ones to diagnose various diseases and to study complex internal body structures such as organs and soft tissues. Owing to these reasons, the global ultrasound diagnostic imaging devices market is estimated to reach a valuation of US$8.3 bn by the end of 2020 from US$6.1 bn in 2014, registering a steady CAGR of 5.3% from 2014 to 2020.
Some of the top players in the global diagnostic imaging devices market are Samsung Electronics Co., Ltd., Siemens Healthcare, Hitachi, Ltd., Toshiba Corporation, General Electric Company, Carestream Health, Inc., and Koninklijke Philips N.V.


The official blog for the informed fan entrenched in the Sawx/Yanks rivalry.
The youth movement in the Bronx is continuing to pay winning dividends. The New York Yankees have won seven games in a row and show no signs of slowing down as they play themselves back into serious playoff contention. Not only are they jockeying for position in the Wild Card standings, where they are currently one game out of the 2nd spot, but are now only three games back of the rival Sawx in the race for the AL East. Yep, this is the same team that held an early "Back to School" sale at the trade deadline. And the result? A record of 24-13 and a month long display of what the future may hold in the Bronx.
After a ten game homerless streak where more than a few commentators starting squawking about Kevin Maas, Gary Sanchez returned to early August form with HRs in each of the last two games. The big hitting catcher also pulled a Kelly Leak when he whacked an intentional ball to deep left center, which was good enough to merit a sacrifice fly. Seriously, good luck pitching around this guy. Brian McCann seems to have adjusted to his new role as DH and back-up catcher with a .313 week highlighted by 3 HRs. McCann should easily eclipse the 20 HR mark for the ninth straight season with one more blast. Jacoby Ellsbury and Brett Gardner are back to early season form hitting .391 and .318, respectively, over the last week. Gardner in particular saved Tuesday night's 7-6 win against the Blue Jays when he tracked down Justin Smoak's line drive to end the game. And Tyler Austin is making a serious case to be the Yankees new full-time first baseman. Next spring, he will have to complete with Greg Bird, who is still recovering from shoulder surgery that has sidelined him this year, but Austin is garnering plenty of favor after Thursday night's game winning HR over the Rays. At the very least, Austin should be the new 4th OF with Aaron Hicks now thankfully dispensable.
Despite my reservations, Masahiro Tanaka continues to impress as the much needed Yankee ace. Tanaka has won two games during the current win streak and has lowered his ERA to just above 3.00. Most importantly, he has proved to very effective against the explosive line up of the Toronto Blue Jays. Over three starts this season, he has a 2.60 ERA which will be counted on again as the Yanks have one more trip planned north of the border. Bryan Mitchell's first start of the year was fruitful as he held the aforementioned Jays scoreless over five innings. And while Michael Pineda and CC Sabathia are having their recurring struggles, Joe Girardi is having no issues using his newly expanded September bullpen to get outs. Luis Severino has blossomed in his new middle relief role. Tommy Layne and Chasen Shreve have found success in their recent outings. And even Blake Parker came up big when he found himself in the unfamiliar role of having to get Dellin Betances out of a jam in Tuesday night's heart-attack inducing 9th inning. Despite the hiccup, Betances should be fine especially with fellow relief ace Tyler Clippard along for back-up.
Another September and the Yankees are playing meaningful baseball. More importantly, they are hot at the right time and not stumbling to the finish line. Not sure they will challenge the 2007 Colorado Rockies 21-1 run but the Yankees are finding many ways to win with a new hero almost every night. There's plenty of baseball and plenty of hope left in the season.


I am available by appointment for healing work at a beautiful studio space (Ridgefield/Danbury, CT) that I created specifically for women. I offer a unique session which combines therapeutic massage, organic creams & essential oils and reiki.
Each and every session includes the use of hydrotherapy, aromatherapy and energy work. Everything was designed to create a healing experience for mind, body and spirit.
Angel and oracle cards are a divination tool (gift!) They offer an intuitive way to help bring clarity, inspiration and wisdom. Divine guidance is always at our disposal but connecting to is a healing art we have forgotten.
I begin every reading with a distance Reiki session, which is in and of itself, a very special healing.
A reading includes the pictures of the cards drawn and a detailed, written reading that is emailed to you for you to keep. A reading is always given with the intention of inspiration, illumination and clarification.
Please use the form below to request a reading and any special information you'd like to consider. Although, sometimes, it is best to trust that the cards will reflect the answers that you seek.


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Artist's midcentury enclave asks $1.9M
New, 1 comment
Huge windows create the perfect art studio
By Megan Barber@megcbarber Mar 2, 2020, 10:30am EST
Photos by John Videler, courtesy of William Pitt Julia B. Fee Sotheby's International Realty
Midcentury homes come in all shapes and sizes, and the latest to catch our eye is this five-bedroom, six-bath home in Westport, Connecticut. Built in 1964, the home features a central A-frame with long horizontal wings off each side of the triangle.
Know of a great House of the Day?
Get thee to the tipline and send us your suggestions.
The airy house also boasts exposed beams, clerestory windows, and floor-to-ceiling glass. In addition to its midcentury credentials, the home is the enclave of artist Marlene Siff and is filled with oversized, brightly colored paintings and sculptures—all for sale along with the house. Siff's studio is a wonder: a light filled oasis with 14-foot walls of glass that look out onto the forest-like backyard.
Other perks in the home include a library, billiards room, a glass-surround tower with epic views, and a sunken conversation pit with fireplace. Outside you'll find a pool, pool house, and tennis court. Interested? 15 Broadview Road is on the market now for $1,900,000.
The airy house has exposed beams and an extensive collection of Siff's paintings and sculptures.
The open-concept kitchen sits adjacent to the living room with bright skylights.
Siff's artist studio has 14-foot walls of glass and clerestory windows with views out to the forest-like yard.
The A-frame section f the home features more of Siff's art in a cozy nook.
The sunken conversation pit features a fireplace, built-in bookshelves, and floral seating.
Frank Lloyd Wright's Final Design, Auctioned Last Year for $1.7M, Now Asks $8M. Huh?
15 gorgeous backdrops for your next Zoom call Want a better video chat background? Try these options, carefully selected to look sufficiently realistic.
Gorgeous algae-filled chandelier also purifies the air The Exhale Chandelier harnesses the power of photosynthesis to remove CO2 from the air and produce oxygen.
Enchanting 'gingerbread house' estate wants $2.2M Located in the town of Tyringham, Massachusetts, Santarella is set on nearly four acres and comprises four separate homes, with the earliest one dating back to the 1750s.


The IHS/hornplayer.net Free Classified Ads are for (French) horns and horn related items only. Irrelevant ads will be deleted immediately and users who post them may be blocked from the site.
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Considering that 100 years ago, doing the laundry involved some serious arm muscles, I feel pretty lucky to be able to toss clothes into a machine and have them come out clean.
That said, keeping a busy household in fresh garments, sheets and towels isn't always easy. For a smooth laundry day (and no more missing socks!), here are 10 tips and tricks. Try one or try them all.


CARFAX One Owner Our 2006 Saturn VUE shown in Deep Blue stands tall and serves up some stiff competition in the compact SUV market! This SUV is roomy and practical and one-ups the others with unique styling, high quality interior finishes, and additional options not offered by others in this price range. Under the hood sits a 3.5 Liter V6 producing an impressive 250hp. Paired with a 5 Speed Automatic transmission, our Front Wheel Drive is super-enjoyable to drive!
The VUE's comfy front bucket seats and a reclining rear bench seat make this a perfect ride fit for five, and gives your rear passengers plenty of legroom. It even has enough room to haul an 8 foot ladder! Take a look at our photos and see just how nicely appointed this immaculate VUE is. Your needed safety features are all in place, too.
This is a GREAT SUV for the money and is going to make one lucky person very happy! Print this page and call us Now... We Know You Will Enjoy Your Test Drive Towards Ownership!


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Ann Radcliffe and the female Gothic genre
Radcliffe's most famous work
One of the most challenging and rewarding courses I've taken is on Ann Radcliffe (1764-1823). Regarded as the principal creator of the female Gothic genre, Radcliffe is credited as establishing a standard and new formula for Gothic fiction which earned it great respect and a larger readership. Patriarchal authority and institutions were challenged and examined in the Female Gothic. Unlike male writers such as Matthew Lewis who wove scenes of sexual assault into their work, Radcliffe's terrors come from implied or possible physical assaults, and the "explained supernatural". Her essay, "On the Supernatural in Poetry", examines how she distinguishes between "terror" and "horror".
We read four of her six novels in class: A Sicilian Romance (1790), The Romance of the Forest (1791), The Mysteries of Udolpho (1794), and The Italian (1797). The Mysteries of Udolpho, which most recently inspired Guillermo del Toro and his movie Crimson Peak, is 672 pages long and is considered Radcliffe's masterpiece.
Radcliffe is a great storyteller with an incredible sense of adventure. I found myself captivated by her exotic settings and intriguing characters. Although few modern readers have heard of her, Radcliffe has influenced several writers including Sir Walter Scott (1771-1832), Jane Austen (1775-1817), Edgar Allan Poe (1809-1849), and more recently, Flannery O'Connor (1925-1964) and Anne Rice (1941).
For more on Radcliffe:
Ann Radcliffe (biography)
Ann Radcliffe (Encyclopedia Britannica)
Ann Radcliffe – An Introduction
The Female Gothic – An Introduction
Davison, Carol M. History of the Gothic: Gothic Literature 1764-1824. Cardiff: University of Wales Press, 2009.
Ellis, Markman. The History of Gothic Fiction. Edinburgh: Edinburg University Press, 2000.
Heiland, Donna. Gothic & Gender: An Introduction. Oxford: Blackwell Publishing, 2004.
Mulvey-Roberts, Marie. The Handbook of the Gothic. New York: New York University Press, 2009.
Wright, Angela. Gothic Fiction: A Reader's Guide to Essential Criticism. New York: Palgrave Macmillian, 2007.
Category: Creative Writing Programs, General, Links, Recommended Books Tagged: Ann Radcliffe, English literature, female gothic, literature, reading, reading and writing, recommended books, writing resources
In Thirteen Ways of Looking for a Poem: A Guide to Writing Poetry, Wendy Bishop states that "contemporary poets prefer rhyme that doesn't call attention to itself; concrete, particular images; and conversational… language." Since starting a course in contemporary poetry, I've been intrigued by Billy Collins' poems. They epitomize the "unexpected phrases and strong sensory details" that Bishop includes in her characteristics of contemporary poetry.
See or hear Collins read his poems by clicking on the following links:
Forgetfulness (animated)
I Chop Some Parsley While Listening to Art Blakey's Version of 'Three Blind' Mice
Some Days (animated)
Walking Across the Atlantic (animated)
Now and Then (animated)
The Trouble With Poetry
Category: General, Recommended Books Tagged: Billy Collins, contemporary poetry, contemporary poets, creative writing, literature, poetry, reading, Wendy Bishop, Writing Poetry
I have a tendency of reading more than one book at a time. Right now, Chang-Rae Lee's A Gesture Life is sitting on my nightstand. It's what I'm reading at home. Judy Fong Bates' Midnight at the Dragon Café is in my bag. It gets read everywhere else, especially in my car as I wait for my daughter during her piano lessons and band practice.
A Gesture Life by Chang-Rae Lee
Lee, a Korean-American writer, immigrated to the States in 1965. A Gesture Life weaves some heavy issues into the storytelling, including the treatment of Korean comfort women by the Japanese Imperial Army during World War II – something the protagonist witnessed during his years of military service. Needless to say, it has a profound effect on him that lasts a lifetime.
Midnight at the Dragon Cafe by Judy Fong Bates
Fong-Bates is a Chinese-Canadian writer. I'm only a couple of chapters into Midnight at the Dragon Café, which was recommended to me by a friend. The book was the 2011 One Book Community Read for the city of Toronto.
Category: General, Recommended Books Tagged: american writers, asian-canadian, books, chang rae lee, cultural stories, korean-american writer, literature, reading, recommended books
April Poems
April flowers bring…
Emily Dickinson said, "If I feel physically as if the top of my head were taken off, I know that is poetry."
Organized and sponsored by the League of Canadian Poets, Canada has acknowledged April as National Poetry Month since 1999. The United States introduced the idea in 1996. Great Britain celebrates October as their National Poetry Month.
Sometimes I struggle through a poem, lost in the words or its meaning. I love the freedom that comes with reading them. There are no right or wrong answers – there shouldn't be. You can read a poem, and it is yours to interpret; yours to personalize. I love how some poems make me feel. Like music, they can provoke me to tears, to laughter. Or they can leave me thinking, reflecting. Sometimes, they even leave me confused and dazed.
A few of my favourites:
The Cross of Snow by Henry Wadsworth Longfellow
A haunting poem. Longfellow's wife burned so badly when her dress caught on fire, she died shortly afterwards.
i carry your heart with me by e.e. cummings
One of the best love poems ever.
I Am in Need of Music by Elizabeth Bishop
A classic.
And in honour of both National Poetry Month and the month of April:
Always Marry An April Girl by Ogden Nash
My favourite Canadian poets include: Leonard Cohen, Michael Ondaatje, Margaret Atwood and Catherine Graham.
More on National Poetry Month:
Celebrate National Poetry Month with CBC Books
National Poetry Month FAQ
Category: General Tagged: literature, National Poetry Month, poems, poetry, reading, writing
Happy, dark, or ironic – Short stories with a twist
Whether they are happy, dark, or ironic, I love twist endings. I stumbled onto "A Letter to God", a short story by Gregorio Lopez Fuentes (translated by Donald A. Yates). It is about a poor farmer named Lencho who sadly loses his crops during a terrible hailstorm. Poor, but a man with strong faith, he writes to God and asks for money to help him get through the winter. The ending made me laugh out loud – it was so nutty. You can click on the story title to read it online.
Some of my other favourite short stories with a twist include "The Necklace" by Guy de Maupassant and "The Story of an Hour" by Kate Chopin.
It takes talent and good storytelling to create a twist ending that works. It can't feel forced or heavy-handed to be effective.
Here are some links you might want to check out:
Five Ways To End With A Twist (written for screenplays but applicable to all genres)
Writing Short Stories With A Twist Ending
Classic Twists (collection of short stories)
Category: Short Writing Tagged: creative writing, literature, reading, short stories, stories with a twist, writing, writing resources
My first e-reader (sigh)
When I was asked if I wanted an e-reader for Christmas, my answer was a flat out, "no". I love the texture of paper pages, the smell of new books and the worn-out feel of second-hand books. I also love walking into bookstores and being surrounded by books of all sizes, shapes, and colours.
But when the second book that I really wanted to read was only available in e-book format, I thought maybe it was time to at least be open to the possibility of an e-reader.
As soon as I walked into my local Chapters bookstore, I was greeted by a friendly Kobo salesperson who happily walked me through his display.
"This is the one I own," he said, and passed me a Kobo Glo.
It didn't feel so bad in my hands, especially when it was put into a case that looked like a book cover.
I wasn't entirely sure if it was a good or bad thing that the reader could customize the font size, justification, margins, and even the line spacing. I knew editors who laboured and agonized over such decisions. The fact that any reader could now arbitrarily change everything seemed somehow wrong.
I love the built-in dictionary though. By pressing any word, I instantly get its meaning. As well, the ability to highlight passages and make notes about them is very cool.
The Kobo Glo also has a built-in light which I'm thinking will be great for reading in my car when I'm waiting for my daughter in the parking lot of her music school.
Still not sure where I stand in the e-book vs traditional book debate. Will find out soon though. I've downloaded a few books including The Ginger Man by J.P. Donleavy which was recommended to me by Donleavy's grandnephew, and hard to find in print version in Toronto.
Category: General, Recommended Books Tagged: books, e reader, e-books, e-readers, reading, recommended books
Black Beauty by Anna Sewell (1877)
"It's told from the point of view of a horse," my daughter, Claire, told me when she started reading Black Beauty. "I don't know if I want to read it." She finished the book today, and concluded that it was a great read, and Black Beauty was a character she wouldn't ever forget.
When Claire asked me to name some memorable characters from books I had read, the first few names that came to mind were:
Nomi Nickel, A Complicated Kindness by Miriam Toews (Canadian)
Sixteen-year old Nomi, who lives in a Mennonite community, is abandoned by both her mother and sister. She asks a question that continues to haunt me: "Is it wrong to trust in a beautiful lie if it helps you get through life?"
Atticus Finch, To Kill A Mockingbird by Harper Lee
As a kid, I had a crush on Atticus, and wondered how many fathers were like him in real life. He was so wise and had the right things to say about everything that mattered: "You never really know a man till you walk a mile in his shoes."
Dunstan Ramsay, Fifth Business by Robertson Davies (Canadian)
I read this book in grade 12 English class. Dunstan's involvement with Mary Dempster, a woman he spends his life trying to make a "saint," is a result of his deep rooted childhood guilt: "I feared to go to sleep and prayed till I sweated that God would forgive me for my mountainous crime… I was alone with my guilt, and it tortured me."
Anne Shirley, Anne of Green Gables by L.L. Montgomery (Canadian)
I remember wondering if I should add an "e" to the end of my name after reading Anne say, "A-n-n looks dreadful, but A-n-n-e looks so much more distinguished."
Iago, Othello by William Shakespeare
I'm wowed by his evil brilliance as much as I fear it. I still get chills reading, "I am not who I am."
Category: General, Recommended Books Tagged: books, canadian writers, literature, memorable characters, reading, recommended books, writers


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'A good book is the precious life-blood of a master-spirit'
Buy from Apple
Buy from Barnes & Noble
Buy from Kobo
Buy from Harper Collins
Buy from Hive
Buy from WHSmith
William Burroughs and James Grauerholz
'Where are the snows of yesteryear. And the speedballs I useta know? Well, I guess it's time for my Ovaltine and a long good night.'
In 1996 William Burroughs began writing a final journal. He died the following summer after a life of notoriety: godfather of the Beat writers, author of thirteen controversial novels, druggy, dangerous and bleak. Spanning the realms of personal memoir, cultural criticism and fiction, Burroughs' diaries include anecdotes and memories, entries on his beloved cats and the joys of housekeeping, and musings on drug-taking, humanity and government cover-ups.
'Last Words' contains some of the most brutally personal prose in the William Burroughs canon, and the deaths of his friends, Allen Ginsberg and Timothy Leary, provide a window onto his own preparations for death – a quest for absolution marked by a profound sense of guilt and loss.
Reviews of Last Words
'He is a writer of enormous richness whose books are a kind of attempt to blow up this cosy conspiracy, to allow us to see the truth.' J. G. Ballard
'These journals make for unbearably poignant reading. Unlikely as it may sound, Bill Burroughs was only human after all.' The Times
'An exploration in depth, and in sum, of Burroughs' personality and creative pre-occupations…[A] rich repetition, with variations, of a string of half-conscious fancies, scenarios and literary allusions. "Last Words" also presents fresh clues to the larger design of his imagination, and a means of gaining a renewed perspective on his work.' New York Times
'"Last Words" reveals the author of "Naked Lunch" riddled with arthritis and still saddled with guilt for shooting his common-law wife in 1951. Although he seems more vulnerable than ever before, the anti-establishment anger continues to flare up at odd moments, his skewed sense of humour still sends out sparks.' Time Out
'There's a savage glamour about William Burroughs, both in his writing and his life…"Last Words", made during the last nine months of his life, shows him to be as sharp-minded as ever.' Ham & High
'Elegiac and filled with a curious kind of contentment at the way things have turned out. For the first and only time, he reveals a gentler self, full of years and filled with grace. He was a great American writer to the end.' Gay Times
'Fascinating. Burroughs surfaces among his words as a bent, acute, watchful, irritated, clever old man, like a sparkling eye peering out from the greasy broken panes of a dilapidated building.' Financial Times
'"Last Words" is filled with memories and reminiscences delivered in staccato poignancy. Burroughs cuts up his recollections and dreams, merging, always playfully, sometimes painfully, fact with fiction…A welcome addition to the extensive Burroughs oeuvre.' Scotsman
More from William Burroughs, James Grauerholz
Naked Lunch: The Restored Text
The Place of Dead Roads
The Ticket That Exploded
Word Virus: The William Burroughs Reader
The Atrocity Exhibition


Written with support from Anthony Gonzalez, Paul Miller, Steven Williams, George Nelson, Timothy Moore, Ryan Lee, Jonathan Nelson, Scott Johnson, Donald Nelson, Charles Johnson, Raymond Davis, William Turner, George Allen, Jack Collins, Frank Rodriguez, Benjamin Allen, Brian Collins, Brandon Perez, Thomas Gonzalez, Andrew Martinez.
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Saturdays kids
Don't be jealous of successful people – befriend them
Maybe the sole purpose of television is to make us feel defeated.
I know I must have felt that way before.
Every time I turn on the channel, a new ad for a car I don't have, a new reality show is shot in a foreign location where I've never been or a movie with a beautiful girl that I will never see..
Social media in exactly the same way.
How many times have you clicked through a Facebook profile picture of a "friend" (whom you've never met) at 1:37 and at least one * teenager * got a little jealous because they seem to be living a much more exciting life than you, good looking people And full of more sense?
And they are always on vacation. Dear God! Why all their pictures from vacation?
Who is giving this cruise out ????
Things like this really bothered me for 3 reasons:
1.) I felt like I was "backward" for my age. At age 24, I had close friends who quickly climbed the corporate ladder and what I thought was big money. And other friends who got a doctorate or professional degree were doing really great, exciting work
I didn't want any of them, but I couldn't help feeling a dweeb talking to them occasionally. When they asked me what I was doing, I could really tell them I was still "working in the restaurant and trying to get things out."
2.) I felt like no one was behind me. I knew I wanted to start my own business (s), but I had no mentor to give me strong advice or a step-by-step action plan. I had no one who knew the rope and could help me if I fell. I wanted someone to tell me "do it, then do it".
Unlike corporate world or graduate school, there are really no guidelines or "best practices" for being an entrepreneur. This is a shit storm you have to find yourself. That's why it's so scary.
3.) I felt like I was in a vacuum. In "real life" I was really the only person I knew who wanted to be an entrepreneur. All the people where I worked… they just wanted to be on the clock দিন day after day.
I wanted to meet others like me.
Who were driven to get more out of their lives and really wanted to create something new.
I could bounce ideas from people and it wouldn't make me feel "weird" to think or talk about personal development.
I always knew that if I was able to fill my whole life with the people I wanted to be, success was pretty much guaranteed. It's just common sense.
Why? Because people almost always meet the expectations set for us – whether the expectations are set by ourselves or others.
This is why stereotypes work.
I have taught college admission preparation (SAT, ACT, etc.) for many years and I can tell you that stereotypes have a statistically significant effect on how minorities and women feel about their math abilities. It has nothing to do with IQ – which varies from person to person.
The real answer is simple: if you're told "statistically speaking", you're going to perform worse than your classmates on a particular subject … guess what? You often do.
There are expectations and your brain meets them. It's not magic, it's science.
Stereotypes can also meet positive expectations for you.
If you live in a family full of professional athletes, hopefully you will excel in sports and possibly become a professional. And what is happening in the end? You see multiple generations of the same family playing professionally year after year. Look at the Manning family in the NFL. Think they're all just "genetically gifted"? This is a prime example of expectation in the workplace + years of training.
So over the past four years, I've broken my ass to connect with other entrepreneurs who are doing amazing things. I've gone out of my way to build mentorship relationships with startup founders, millionaires, and high-performance executives in all fields.
Because I was sick of feeling jealous of those who are doing well.
I realized that if I wanted to be one of them, I had to do whatever it took to get on their peers.
And I did. But it certainly didn't happen overnight.
You need more than luck to succeed in affiliate business. But when I was finally able to build a huge community of super-valuable people around me, I noticed something incredible: it was almost impossible to fail.
If I had a question, doubt or fear, there was a pre-formed community around me who actually wanted to help me. If I didn't know which way to turn, there was a consultant at Speed Dial for any problems I might encounter.
And above all, there was a new level of expectation on my part by those around me. They just hoped I'd do better, and I didn't want to disappoint them.
Turned out I was making an entrepreneur in me all the time?
Creating that community of friends and mentors was the ultimate tool for kicking my progress fast – and perhaps the biggest reason I was able to move from one day job to self-employment so quickly.
By Ethel Thomas
There are 3 reasons why your idea doesn't stop (and what to do about it).
How I gave my business idea to Mark Cuban without going to 'Shark Tank'
Franchising as a young entrepreneur: Why it makes sense
Who is the CEO of Bang
When was YouTube created? – Under 30 CEO
How to get the rare Tesla Tequila
How long is a business day? 7 tips to get through
© 2023 Saturdays kids. All Right Reserved Lawman Blog By Moral Themes


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Dogwood State Bank
4051 S. Memorial Drive
Dogwood State Bank got its start in the spring of 2000 when several Morehead City bankers began to talk about starting a community bank. They felt that a bank that was locally owned and operated could… read more
Dogwood State Bank got its start in the spring of 2000 when several Morehead City bankers began to talk about starting a community bank. They felt that a bank that was locally owned and operated could provide better personal service, especially with decisions being made locally by people who live and work in and are committed to the community. They also felt that money deposited in the bank should be lent locally to help the community grow and prosper. It would also be important for employees to be dedicated to giving back to the community through local involvement. The name ''Dogwood State Bank'' was chosen to reflect the flavor of the coastal area as well as the solid foundation of the bank.
We are located in the Albemarle Center complex at the corner of Memorial Blvd and Chapman Rd next to UHAUL. We are down the street from Pitt Community College.


PLEASE DO NOT bring wine to winemaker events, unless otherwise specified.
Corner 103 Wine Club Members will receive a special promo code to get the Member Special Pricing.
Suite D Social Club and RATW Wine Club Members should already have your special code.
Any questions, please contact Jenn at 707-933-3000 x10 Monday - Friday.


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Accenture Teams with SAP to Develop Cloud Solution to Digitally Transform Oil and Gas Upstream Operations
New solution includes artificial intelligence, mobility and blockchain capabilities that provide full transparency into operations and cash flow
NEW YORK; Sept. 18, 2018 – Accenture (NYSE: ACN) is expanding its collaboration with SAP in the oil and gas industry by co-developing an SAP® S/4HANA Cloud® solution designed to help oil and gas companies significantly cut operational costs and create new revenue opportunities.
In an environment of volatile oil prices, sustained bottom-line pressure, and changing energy portfolios, upstream oil and gas operators are increasingly looking to innovate and simplify operations with standard processes and automation. The new SAP S/4HANA Cloud solution for oil and gas companies is a portfolio of intelligent cloud services that set new industry standards with more transparency into operations and cash flow through cutting-edge technologies including artificial intelligence (AI), mobility and blockchain. The solution is available via both public and private cloud.
"The oil and gas industry has traditionally been a leader in deploying intelligent technologies," said Jan van den Bremen, senior managing director and global technology officer for the Accenture Resources operating group, which serves the chemicals, oil and gas, natural resources and utilities industries. "Working with SAP and a consortium of industry leaders, we are co-innovating on this new solution and harnessing the flexibility of SAP S/4HANA Cloud to add agility and scale to operational processes, from finance to asset management to field logistics, helping companies transform into intelligent, digital enterprises."
The ready-to-use solution is being developed with input from a consortium of oil and gas industry leaders, including Shell, and is based on industry standards. "Delivery of oil and gas industry requirements within SAP S/4HANA Cloud will enable us to accelerate our strategy towards a standard SaaS ERP platform. We are very happy to see SAP collaborate with Accenture to develop these industry requirements, given Accenture's 40 years of experience in the oil and gas industry," said Frank Westerhof, general manager, Enterprise Platforms, Shell.
"SAP and Accenture are committed to helping oil and gas companies gain better visibility into their operations and transform into intelligent enterprises," said Benjamin Beberness, global vice president, Industry Business Unit Oil and Gas at SAP. "We expect that this new solution will help oil and gas companies maintain high standards and cut costs with the flexibility and agility that comes with a cloud deployment."
The Accenture and SAP alliance spans more than 40 years. Accenture is SAP's first alliance partner to co-develop core and industry solutions based on SAP S/4HANA. Accenture has one of the world's largest teams skilled in SAP solutions and services, with more than 47,000 practitioners globally, and has received 38 SAP Pinnacle Awards for excellence in developing innovative solutions that help clients run their businesses better and more efficiently.
SAP, SAP S/4HANA and other SAP products and services mentioned herein as well as their respective logos are trademarks or registered trademarks of SAP SE (or an SAP affiliate company) in Germany and other countries. See http://www.sap.com/corporate-en/legal/copyright/index.epx for additional trademark information and notices.
All other product and service names mentioned are the trademarks of their respective companies.
Julie Bennink
julie.l.bennink@accenture.com
Guy Cantwell
guy.cantwell@accenture.com


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Вя́йнё Ху́хтала (; род. 24 декабря 1935, Сийкайоки, Финляндия - 18 июня 2016, Йямся, Финляндия) — финский лыжник, олимпийский чемпион, призёр чемпионатов мира. Брат лыжника Эйно Хухталы.

Карьера
На Олимпийских играх 1960 года в Скво-Вэлли, завоевал золотую медаль эстафете, кроме того был 13-м в гонке на 15 км. В том же году первенствовал на Шведских лыжных играх в Фалуне.

На Олимпийских играх 1964 года в Инсбруке, завоевал серебро в эстафетной гонке, а также занял 4-е место в гонке на 15 км и 14-е место в гонке на 30 км.

На чемпионате мира-1962 в Закопане завоевал серебряную медаль в эстафете, а также был 13-м в гонке на 15 км.

На чемпионатах Финляндии побеждал 6 раз, 2 раза в гонках на 15 км (1961 и 1962) и 4 раза в эстафете ((1960, 1961, 1963, 1964).

Кроме лыжных гонок серьёзно занимался академической греблей, в частности, трижды побеждал на чемпионатах Финляндии (1957, 1958 и 1960) в классе парных четвёрок лёгкого веса без рулевого.

Примечания

Ссылки

Лыжники Финляндии
Олимпийские чемпионы от Финляндии
Олимпийские чемпионы по лыжным гонкам
Чемпионы зимних Олимпийских игр 1960 года
Серебряные призёры зимних Олимпийских игр 1964 года
Лыжники на зимних Олимпийских играх 1960 года
Лыжники на зимних Олимпийских играх 1964 года
Гребцы (академическая гребля) Финляндии
Спортсмены нескольких видов спорта


For over 100 years, Speed Queen has been a leading manufacturer of laundry equipment, providing great quality and technology that has revolutionized the industry.
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For a high quality washer or dryer that's built to last, consider Speed Queen. Universal Appliance and Kitchen Center offers a great selection of Speed Queen laundry machines, helping you find the perfect addition to your home or business. Be sure to browse our impressive selection of laundry appliances today.


Invisalign treatment has become a popular alternative to traditional braces for correction of crooked or slightly misaligned teeth. Recent evidence indicates that Invisalign can also be effective in correcting minor malocclusions, or a misaligned bite. Our dental office offers Invisalign in the San Antonio area, and we can help you determine if your malocclusion can be effectively treated in this manner.
Traditional orthodontics to correct crooked teeth or misalignment uses metal braces affixed to the teeth. The braces are connected with rubber bands that create pressure on the braces to move the teeth into the appropriate place. For major malocclusion or extremely crooked teeth, braces are still often the most effective option. However, for less severe alignment problems, Invisalign can be a preferred option.
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Not all misalignments and malocclusions can be effectively treated with Invisalign. The best way to determine if Invisalign is a good option for you is to schedule a consultation with your dentist to discuss your needs and goals. If your dentist believes your goals can be met through Invisalign treatment, then you will move on to discuss how long your treatment will last and how to ensure the best long term results. You will have to wear your aligner trays at least twenty-two hours every day, and after treatment is completed, you will wear a retainer periodically as recommended by your dentist to be sure your teeth remain in the proper position.
To find out more about how Invisalign can correct your misalignment or minor malocclusion, please contact our office to schedule a consultation.


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Blog: Montreal Qualifying
Date: 7th June 2015 at 9:48pm
No alarms and no surprises
He said he was surprised. But possibly he was the only one.
Lewis Hamilton claimed a decisive pole position for today's Canadian Grand Prix and at no point did it feel like a shock. Almost from the first moment wheels turned on the Île Notre-Dame this weekend it was clear that the Mercedes power unit was the thing to have. And of course chief among them were those in the works cars.
Then we have Lewis himself, for whom the Montreal track is the happiest of hunting grounds.
It all had a nice symmetry to it also. It was pole number 44 of Lewis's career for car number 44, also here where in addition to his strong record he also took his first F1 pole and win eight years ago. It's pole number six for him from seven rounds this season too. Some things just seem to have their own inevitability.
Not that we should forget where we are. The Circuit Gilles Villeneuve, almost the sport's equivalent of the Bermuda Triangle. Where things are bound to happen. And in advance of yesterday's qualifying session they happened to Lewis.
Almost unbelievably he finished up in the Saturday morning practice session bottom of the pile and close to six seconds off the pace, which was a consequence of on both of his runs flat-spotting the tyres under braking in the cooler temperatures and having abandon the run. This meant that he could not at all refine his set-up or approach from Friday going into the qualifying hour.
'I even surprised myself really just how quickly I got back to it' he said later.
'We did a longer first run in Q1 which enabled me to get back onto it. And it was relatively easy to be honest.'
Ah, now we're getting closer to how it appeared to the rest of us. Almost immediately indeed in the qualifying session Lewis was markedly the quickest out there. There was a tiny doubt when in his first Q3 effort he lost two tenths to his team mate Nico Rosberg in sector one, a consequence apparently of some snap oversteer, but by the end of the lap he'd taken that back and more and was under Rosberg's time by a clear three tenths. The next time around Lewis claimed the lost sector one time back thanks very much and shaved 0.177 off his previous mark. Nico didn't improve (more of that anon) and that was that.
Ferrari some thought, with a much-vaunted engine upgrade, might be able to do something. But if silver supremacy in qualifying looked extremely likely in advance once Sebastian Vettel had fallen out of things early, it was certain.
Vettel having appeared as normal in Q1 disappeared almost as quickly, due to electrical problems. He reemerged late in that session but seemingly in desperation as the car was far from healed. Two last-gasp efforts didn't really get him close to making Q2. It got worse for him too as an additional five-place grid drop awaited him for overtaking Roberto Merhi under red flags in the morning practice session. Vettel claimed the Manor was going especially slowly but those that saw the footage thought not. It seemed an extraordinary oversight by Seb whatever was the case, given many reckon he is possessed of the finest brain in the sport.
Seb while downbeat at it all still was hopeful of progress in the race from starting in eighteenth. 'We have plenty of tyres and anything can happen' he said. 'We have a strong car and a strong pace, so I should be able to score good points.'
This meant that for Lewis his team mate Rosberg was the only credible challenger and while he as ever kept him honest, occasionally pipping him, in the end he rather presented his team mate with a gaping net. Not that he needed it.
His first set of tyres used in Q3 he said were 'bad', offering little rear grip. And while his team recommended initially that he keep the set-up as it is on the basis the next set would be better Nico reckoned that would be 'just guesswork, I had to adapt the car', and took front wing off accordingly. Next time out sure enough the tyres were back as normal and Nico's car now understeered due to the bum steer set-up change. He didn't improve his time on his second effort ('It was a good lap, just slow' he said) and indeed at no point of Q3 improved on his Q2 time. He admitted afterwards to 'chasing your tail' on the matter.
'It's amazing how it goes' he rued. There are these tiny things in the end that make such a big difference. Today it got me, another time it'll be another driver. Everything just has to come together to get pole position in F1, it's such a fine line'.
'The race is tomorrow, so still some good chances' he added. 'Here it's possible to overtake, so it's not all over yet.'
'There could be some surprises'.
Both Hamilton and Merc boss Toto Wolff admitted to being pleasantly surprised by the gap to the red cars, which they expected to be around two tenths when in fact it was upwards of three times that. Still, as Wolff cautioned, with Vettel sitting it out in effect Ferrari likely hadn't quite shown the full content of its hand.
Kimi Raikkonen in the other Ferrari self-admittedly now is struggling in qualifying, but there was enough of a gap between the Mercedes and the rest for him to just slot himself into, for a P3 start. It's his best Saturday result of the season and as the man himself outlined afterwards there are a few reasons not to count him out for tomorrow.
'Tomorrow is the race, usually we are a bit stronger there.
'So we have to make a good start and go from there and see what comes out. There's still quite a bit of work to be done as a team to be exactly where we want to be – in the front. But y'know, it's one of those days. You're not happy but in a way it's been better than in the past.
'We can only do our best and we will see in the race, obviously, what will happen but we were pretty OK in the long runs so I feel fine with it. But obviously the race is a different story and yes, we have all the Mercedes cars in front of us but it shows something, that we are doing something right to get there. But there's still work to be done. Who knows? We will try to beat all of them and see what happens.'
As mentioned Montreal this weekend was Mercedes country and this was underlined by seven of the top ten in the qualifying order being so powered. Indeed likely all of them would have made it but for Felipe Massa also having to bow out in Q1, in his case with a lack of engine power, a consequence of a loose fixing in the wastegate.
Valtteri Bottas's Williams was best of the rest in P4, under a tenth over Kimi's best, while the two Lotuses were next up and also close at hand, with Romain Grosjean ahead. Grosjean indeed thought that third place may have been possible but for a strange team mix-up which led to both Lotus cars being released from the garage at almost precisely the same moment for their final runs, to the point almost of tripping over each other.
'It was just a misunderstanding between the two timing going out of the garage' said Grosjean later.
'It didn't help the preparation of the last lap. I thought I could take the slipstream from Pastor but I was too far [away] at the start of the lap. [With] perfect preparation, perfection slipstream, because you can easily gain a tenth on the back straight, I could have probably been P3.'
Nico Hulkenberg in the Force India was next up in P7, very happy that there was nothing left on the table in his best lap, while his team mate Sergio Perez was tenth and he rued that he couldn't replicate his Q2 pace, which would have been good enough for P8. The Silverstone team often shows up well here.
In between them we had the Red Bulls and both of its drivers were jarringly frank later about the team's current woes. Daniel Ricciardo indeed painted the picture of a team rather lost, that it doesn't have a grasp of how to improve the car at the moment and that various recent upgrades hadn't worked. It's all a long way from the Milton Keynes team's recent pomp, when everything it did seemed to show up on the stopwatch pretty much immediately.
Another under fire was Honda, when just a day after its motorsport boss Yasuhisa Arai had spoken with great optimism about the developed unit it had brought here it had the ignominy of Jenson Button sitting qualifying out thanks to an engine change while Fernando Alonso with a change of his own didn't appear in Q3 until late and only managed three laps. Arai had to bat away rather searching questions later. He still insists that matters will become more conspicuously good in the second half of the year.
Of course the matter of who is to be at the front right now has a lot less of whistling in the dark about it. Even here. Especially here. We know what they say about races at Montreal, but you suspect it'll have to do its worst and more for Lewis Hamilton to be deprived of victory today.


A little girl's doll is probably the last thing you'd expect to be the most talked about moment of the night during the final game of Australia's Rugby League. But when the two-year old daughter of Johnathan Thurston, captain of the winning team North Queensland Cowboys, came onto the pitch to celebrate carrying a dark-skinned doll, it was a highly symbolic and emotionally-charged moment for many. For the first time in the league's history, the captains of the two teams during the football final were indigenous Australians — at a time when Australian sport is rife with racism against indigenous players, and the country is having a heated debate on the constitutional rights of its indigenous people. In that context, the image of a little girl carrying a dark-skinned doll was hailed by many as a beautiful moment of diversity.


Demographic data – When do I get it for my account?
You are here: Home / Plus-poster / Demographic data – When do I get it for my account?
Most of my Swedish customers have now got the demographic reports in their Analytic accounts, and I'm totally excited and thoroughly supprised over how good they are.
Yet, I'm frustraded that I haven't got this in my own account, which means that I can't blog about these great new features in Analytics. Does anyone know when this will be rolled out to all accounts?
I feel your frustration! There doesn't seem to be any pattern to the way it's rolling out to accounts, but it's happening and it's happening pretty quickly.
I wasn't even aware of this. This would indeed be brilliant… when, when, when in the UK?
Make sure you are using DoubleClick integration (dc.js) – https://support.google.com/analytics/answer/2444872 as well as enabling demographic and interest report in your web property.
"Your Demographics and Interest Reports have been enabled, but your Analytics tracking code does not include the necessary support to show them." – what should I do in my Google Tag Manager?
Yeah. My mistake. Currently is Universal Analytics (analytics.js) and I need to set dc.js.
Has anyone an idea how good those estimates are? I guess they are using the same data as for AdWords interest based categories and to my experiences the targeteting can sometimes be good, and sometimes seem way off (considering ctr and other parameters).
+David Stockelberg it's using DoubleClick data – the same type of info you would use to target audience. It can't be perfect but it's directional.
+David Stockelberg +Stephane Hamel I think it is good that you can segment your data and see what are your audience's demographic data for users which are creating conversions instead of these who are not. So you can use it for AdWords to create your ads.
+Stephane Hamel "Directional"? Question is: is it actionable? Which relates to it´s truthfulness (which in a pragmatist concept of knoweldge leads us back to actionability, anyway I won´t dwell on philosophical questions here).
I guess Google has made research to make sure it´s not way off and gives a totally wrong picture. So the machines probably do a pretty good job. But all statistics and averages depends a lot on volume, and I´d guess for many smaller sites the stats actually give a wrong (un-actionable) picture. The bigger the volume, the better the picture, I presume. But that will also depend on your goals and actions. For an ecommerce advertiser in a very competitive segment with razorthin margins that need to know if they should higher their bids with 2%, it might not be a good idea to use this data even if they have high volume of visit/ors? I don´t know.
i also had that problem and when asking why i still don't have it they always delete my post.
+Jakob Aindreas Rivera – some of your posts were removed because they are very basic or repetitive questions already addressed several times earlier. A simple search on Google or in this community would provide the answers you are looking for.
+Jakob Aindreas Rivera kind in mind most new features are gradually being deployed, so it might take some time before it becomes available to you.


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HALIFAX -- A teacher who co-authored an independent report on inclusive education in Nova Scotia says students with learning challenges cannot succeed under the current program, which is why more funding is needed to transform the province's approach to education.
Adela Njie, one of three commission members who spent a year drafting a five-year plan for reform, told a news conference Monday there weren't enough supports in place to help one of her students, who rarely came to school because of severe anxiety.
"There are numerous examples of students who today are not able to be successful because we have a rigid program," she said. "This is why we need a new model: to meet the needs of the students when they need it and where they need it."
The commission estimates about a third of the province's 118,000 students need some form of support at school.
Their 124-page report recommends more funding for psychologists and behaviour support teachers, and calls for faster student assessments and behaviour intervention.
The report also recommends increasing funding for inclusive education over five years, with the province spending $70 million to $80 million annually by year five, a seven per cent increase for the Education Department's overall budget.
It also recommends hiring 30 behaviour support teachers, 12 school psychologists and 12 regional health nurses this September, as well as paying for 400 school psychology and 200 speech-language pathology student assessments.
In total, the report recommends hiring between 600 and 700 more specialists by 2022.
Nova Scotia's inclusion policy requires the province to make sure students with special needs are given support to succeed in a regular classroom setting.
It's been 20 years since the policy has been reviewed.
That's why the commission is calling for an updated policy and short-term fixes to help priority students in the upcoming school year.
The commission was charged with producing the study in the wake of a bitter labour dispute last year between the provincial government and 9,300 public school teachers.
When the province introduced a bill to impose a collective agreement, many teachers came forward to suggest the inclusion policy wasn't working. Some made emotional pleas about violence in classrooms and having to deal with 30 students with vastly different needs.
Education Minister Zach Churchill said the government has broadly accepted the objectives in the report, but he said flexibility will be required as it works with its partners, including the Nova Scotia Teachers Union.
He acknowledged that implementing the short-term recommendations will be a challenge because there aren't enough specialists to fill the positions.
"In Atlantic Canada, there's actually a deficit in those specialist positions," Churchill said. "We have to work with our post-secondary partners to ensure that we're producing more graduates to meet those needs."
Churchill said the government has already established a bachelor of education steering committee with the post-secondary schools that offer the programs.
The government earmarked $15 million in last week's provincial budget to help implement the report, which Churchill said is in line with the commission's recommendations for this year.
Teachers union president Liette Doucet said the union came up with the idea for an independent commission after concerns were raised by teachers and others.
"In many ways, today's report validates the stand that teachers have been taking over the past two years," Doucet said in an emailed statement.
"It is clear they conducted a full and thorough consultation and have captured the concerns teachers, parents, students and stakeholders have been voicing now for several years."
The report also recommends setting up a "multi-tiered system of supports," saying 80 to 90 per cent of students get the help they need to succeed, while five to 10 per cent need more targeted help, and one to eight per cent need more intensive interventions. It said similar models have worked in Nunvut and Saskatchewan.


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Beijing Impression > Beijing Attractions > Qufu
Confucius Temple in Qufu
Confucius (551B.C.-419B.C.) is the founder of Confucianism. In the second year after he died, his mansion was reconstructed into the Confucius Temple.
Confucius Family Mansion
Confucius Family Mansion located to the east of the Confucius Temple is the resident of Confucius family. First built in Song Dynasty, it has been repaired and enlarged for many times.
Confucius Family Graveyard
Confucius Family Graveyard also called Sacred Graveyard is located outside of the north gate of Qufu City. Occupying about 3000 mu, with a bounding wall of 7km in length, it is the family graveyard for Confucius and his descendents.


Re: Cert only authentication (EAP-TLS) 05-07-2013 01:00 PM Thanks, you were right, and I'd already done those steps, just hadn't removed the domain-controller from the Authentication sources list. now the only thing it can test is the cert.... Get your Wednesday morning in Scottsdale off to a vigorous start by joining us for the 2017 NAC Concession & Hospitality Expo Annual Fun Run/Walk. This annual event starts at 7 a.m. so there is plenty of time to get some exercise in and be ready for a value-packed day at Expo. Dawn Majewski, CCM, of Marcus Theatres is once again heading up the event.
Submit your application early, we tend to get a lot of "procrastinators" and get swamped the last week in December. Early applicants will get processed more quickly. If we need clarification, we will strive to contact you prior to the deadline date. If you wait to submit until December and we find your application to be non-approvable, you risk having to reinstate with penalty costs... The American Association of Nurse Assessment Coordination (AANAC) is a non-profit membership organization representing more than 15,000 long-term care nurses and healthcare professionals who provide quality care for nursing home residents across the nation.
Cisco Support Community. Directory. Network Infrastructure. WAN, Routing and Switching... Order a Certificate of Title or a survey or strata plan for any property in Western Australia. Learn about property ownership, lodging forms and strata reform.
dot1x GPO config has nothing to do with the cert encountered during NAC agent download. That cert is from the https connection to ISE. To avoid that cert, you need to have proper cert selected for Client Provisioning portal. The cert need to have ISE hostname as common name (or wildcard) and either be signed by a trusted public CA, or enterprise CA that the clients trust.
Anyconnect loaded on the Mac to get the cert (is this possible??) Manually install the root certificate and then request/install the user certificate (what they want to avoid) Which (if any) of those options is most reasonable, or is there a better way?
Cisco NAC Appliance Enforcing Host Security with Clean Access Authenticate, inspect, remediate, and authorize end-point devices using Cisco NAC Appliance Jamey Heary, CCIE® No. 7680 Contributing authors: Jerry Lin, CCIE No.
You must graduate from a POST certified academy and meet the eligibility requirements listed under NAC 289.110. If you are employed with an agency, you will be sent to an academy. Once you've graduated, your agency point of contact will complete the process for your certification.