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Lingchong You
Professor of Biomedical Engineering
The You lab uses a combination of mathematical modeling, machine learning, and quantitative experiments to elucidate principles underlying the dynamics of microbial communities in time and space and to control these dynamics for applications in computation, engineering, and medicine.
Current Research Interests
Quantitative biology, synthetic biology, machine learning, antibiotic resistance, microbiome
Current Appointments & Affiliations
Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 2019
Associate Professor in Molecular Genetics and Microbiology, Molecular Genetics and Microbiology, Basic Science Departments 2016
2355 CIEMAS, 101 Science Drive, Durham, NC 27708
Box 90281, Durham, NC 27708-0281
Youlab homepage
Education, Training, & Certifications
Ph.D., University of Wisconsin at Madison 2002
M.S., University of Science and Technology of China (China) 1997
B.S.E., Chengdu University of Science and Technology (China) 1994
Duke Appointment History
Associate Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 2012 - 2018
Assistant Professor of Biomedical Engineering, Biomedical Engineering, Pratt School of Engineering 2005 - 2011
OCT 3, 2019 Pratt School of Engineering
Machine Learning Is Providing New Insights When the Biology Is Too Complex for Traditional Research
DEC 5, 2018 Pratt School of Engineering
Distinguishing Resistance from Resilience to Prolong Antibiotic Potency
FEB 21, 2018 Pratt School of Engineering
New Tool Tells Bioengineers When to Build Microbial Teams
DEC 7, 2017 Center for Genomic and Computational Biology
Where Are All the Germs? Students Explore Duke's Microbiome
MAY 2, 2016 WFDD-Winston-Salem's "SciWorks Radio"
Lingchong You: Tales of the genetically altered, superhero bacteria swarmbot
Time Cues Help Keep Humans Looking Human
Antibiotics Don't Promote Swapping of Resistance Genes
MAR 2, 2016 Gizmodo
Biologists turn bacteria into 'swarmbots'
Bioengineers Design 'Swarmbot' Cells That Die If They Leave the Confines of Their Capsule
Why Do Dividing Cells End Up the Same Size?
OCT 27, 2014 BBC News
Lingchong You comments: Paper test can detect Ebola strains
Dispersal Patterns Key to Invasive Species' Success
NOV 11, 2013 The News & Observer
Chinese-Americans protest Kimmel skit in front of ABC station in downtown Raleigh
Growing Bacteria Keep Time, Know Their Place
Faculty Early Career Development (CAREER) Program. National Science Foundation. 2010
Fellowships for Science and Engineering. David and Lucile Packard Foundation. 2006
Selected Grants
Duke KURe Program awarded by National Institutes of Health 2013 - 2023
RoL: Modulation of microbial community dynamics by spatial partitioning awarded by National Science Foundation 2019 - 2023
Personalizing prebiotic therapies that target human gut microbiota awarded by National Institutes of Health 2017 - 2022
Summer Scholars Program in Genome Sciences and Medicine awarded by National Institutes of Health 2017 - 2022
University Training Program in Biomolecular and Tissue Engineering awarded by National Institutes of Health 1994 - 2022
A comprehensive research resource to define mechanisms underlying microbial regulation of host metabolism in pediatric obesity and obesity-targeted therapeutics awarded by National Institutes of Health 2016 - 2021
Dynamics of horizontal gene transfer in response to antibiotic treatment awarded by National Institutes of Health 2017 - 2021
Post-antibiotic effect and design of optimal antibiotic dosing protocols awarded by National Institutes of Health 2011 - 2021
A synthetic-biology approach to study scaling properties of self-organized patterns awarded by National Science Foundation 2014 - 2020
Bioinformatics and Computational Biology Training Program awarded by National Institutes of Health 2005 - 2020
Understanding the Skin Microbiome through the Integration of Metagenomics, Bioinformatics 2014 - 2019
Programming bacteria for materials fabrication awarded by Office of Naval Research 2015 - 2019
Temporal E2F Dynamics and Cell-Fate Decisions in Single Mammalian Cells awarded by National Institutes of Health 2014 - 2019
Enhancing imaging capability for quantitative and synthetic biology research and education awarded by Office of Naval Research 2018 - 2019
Reprogramming cellular behavior with synthetic gene circuits 2008 - 2018
Supplement - Understanding the Skin Microbiome through the Integration of Metagenomics, Bioinformatics... 2018
A synthetic biology approach to analyze evolution of programmed bacterial death awarded by National Institutes of Health 2014 - 2018
DURIP: Enhancing capability of quantitative biology research and education awarded by Office of Naval Research 2015 - 2016
Development of a Microfluidic Platform to Analyze Evolution of Programmed Bacterial Death awarded by Army Research Office 2014 - 2015
Engineering Microbial Swarmbots awarded by National Science Foundation 2010 - 2015
Delivery of Bacterial Therapeutics to Solid Tumors awarded by National Science Foundation 2009 - 2013
Coordinating Cell Killing by Communication: Biological Control and Cancer Therapy awarded by National Institutes of Health 2006 - 2012
High-performance Computing System for Bioinformatics awarded by National Institutes of Health 2009 - 2010
Modeling, Predicting, and Reprogramming Dynamic Cellular Networks awarded by National Science Foundation 2006 - 2008
BGI Genomics, Shenzhen, China
China Agricultural Univeristy
Joint Center for Life Sciences at Peking University and Tshinghua University
Shenzhen Institute of Advanced Technologies, Shenzhen, China
Publications & Artistic Works
Kim, Kyeri, and Lingchong You. "Bacterial Aggregation Leads to Collective Elimination.." Trends in Microbiology, January 6, 2020. https://doi.org/10.1016/j.tim.2019.12.001.
Zhu, Kui, Shang Chen, Tatyana A. Sysoeva, and Lingchong You. "Universal antibiotic tolerance arising from antibiotic-triggered accumulation of pyocyanin in Pseudomonas aeruginosa.." Plos Biology 17, no. 12 (December 16, 2019). https://doi.org/10.1371/journal.pbio.3000573.
Lopatkin, Allison J., Jonathan M. Stokes, Erica J. Zheng, Jason H. Yang, Melissa K. Takahashi, Lingchong You, and James J. Collins. "Bacterial metabolic state more accurately predicts antibiotic lethality than growth rate.." Nature Microbiology 4, no. 12 (December 2019): 2109–17. https://doi.org/10.1038/s41564-019-0536-0.
Tsoi, Ryan, Zhuojun Dai, and Lingchong You. "Emerging strategies for engineering microbial communities.." Biotechnology Advances 37, no. 6 (November 2019). https://doi.org/10.1016/j.biotechadv.2019.03.011.
Dai, Zhuojun, Anna J. Lee, Stefan Roberts, Tatyana A. Sysoeva, Shuqiang Huang, Michael Dzuricky, Xiaoyu Yang, et al. "Versatile biomanufacturing through stimulus-responsive cell-material feedback.." Nature Chemical Biology 15, no. 10 (October 2019): 1017–24. https://doi.org/10.1038/s41589-019-0357-8.
Wang, Shangying, Kai Fan, Nan Luo, Yangxiaolu Cao, Feilun Wu, Carolyn Zhang, Katherine A. Heller, and Lingchong You. "Massive computational acceleration by using neural networks to emulate mechanism-based biological models.." Nature Communications 10, no. 1 (September 25, 2019). https://doi.org/10.1038/s41467-019-12342-y.
Shi, Xiangguang, Lingchong You, and Ruo-Yu Luo. "Glycolytic reprogramming in cancer cells: PKM2 dimer predominance induced by pulsatile PFK-1 activity.." Physical Biology 16, no. 6 (September 18, 2019). https://doi.org/10.1088/1478-3975/ab3f5a.
Ma, Helena R., and Lingchong You. "Polar-opposite fates.." Nature Chemical Biology 15, no. 9 (September 2019): 850–52. https://doi.org/10.1038/s41589-019-0337-z.
Simon, Joseph R., Seyed Ali Eghtesadi, Michael Dzuricky, Lingchong You, and Ashutosh Chilkoti. "Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells.." Molecular Cell 75, no. 1 (July 2019): 66-75.e5. https://doi.org/10.1016/j.molcel.2019.05.010.
Song, Wenchen, Hai-Xi Sun, Carolyn Zhang, Li Cheng, Ye Peng, Ziqing Deng, Dan Wang, et al. "Prophage Hunter: an integrative hunting tool for active prophages.." Nucleic Acids Research 47, no. W1 (July 2019): W74–80. https://doi.org/10.1093/nar/gkz380.
Luo, Nan, Shangying Wang, and Lingchong You. "Synthetic Pattern Formation.." Biochemistry 58, no. 11 (March 2019): 1478–83. https://doi.org/10.1021/acs.biochem.8b01242.
Wu, Feilun, Allison J. Lopatkin, Daniel A. Needs, Charlotte T. Lee, Sayan Mukherjee, and Lingchong You. "A unifying framework for interpreting and predicting mutualistic systems.." Nature Communications 10, no. 1 (January 16, 2019). https://doi.org/10.1038/s41467-018-08188-5.
Sysoeva, T. A., Y. Kim, J. Rodriguez, A. J. Lopatkin, and L. You. "Growth-stage-dependent regulation of conjugation." Aiche Journal, January 1, 2019. https://doi.org/10.1002/aic.16848.
Meredith, Hannah R., Virgile Andreani, Helena R. Ma, Allison J. Lopatkin, Anna J. Lee, Deverick J. Anderson, Gregory Batt, and Lingchong You. "Applying ecological resistance and resilience to dissect bacterial antibiotic responses.." Sci Adv 4, no. 12 (December 2018). https://doi.org/10.1126/sciadv.aau1873.
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Dong, Peng, Manoj V. Maddali, Jaydeep K. Srimani, François Thélot, Joseph R. Nevins, Bernard Mathey-Prevot, and Lingchong You. "Author Correction: Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.." Nat Commun 9, no. 1 (November 13, 2018). https://doi.org/10.1038/s41467-018-07169-y.
Feng, Y., K. E. Marusak, L. You, and S. Zauscher. "Biosynthetic transition metal chalcogenide semiconductor nanoparticles: Progress in synthesis, property control and applications." Current Opinion in Colloid and Interface Science 38 (November 1, 2018): 190–203. https://doi.org/10.1016/j.cocis.2018.11.002.
You, Lingchong, and Eriko Takano. "Synthetic Biology: Reports from CSHA 2016 and More.." Biotechnology Journal 13, no. 5 (May 2018). https://doi.org/10.1002/biot.201800160.
Lee, Anna J., Shangying Wang, Hannah R. Meredith, Bihan Zhuang, Zhuojun Dai, and Lingchong You. "Robust, linear correlations between growth rates and β-lactam-mediated lysis rates.." Proceedings of the National Academy of Sciences of the United States of America 115, no. 16 (April 2, 2018): 4069–74. https://doi.org/10.1073/pnas.1719504115.
Tsoi, Ryan, Feilun Wu, Carolyn Zhang, Sharon Bewick, David Karig, and Lingchong You. "Metabolic division of labor in microbial systems.." Proceedings of the National Academy of Sciences of the United States of America 115, no. 10 (March 2018): 2526–31. https://doi.org/10.1073/pnas.1716888115.
Marusak, Katherine E., Julia R. Krug, Yaying Feng, Yangxiaolu Cao, Lingchong You, and Stefan Zauscher. "Bacterially driven cadmium sulfide precipitation on porous membranes: Toward platforms for photocatalytic applications.." Biointerphases 13, no. 1 (February 9, 2018). https://doi.org/10.1116/1.5008393.
Dong, Peng, Carolyn Zhang, Bao-Tran Parker, Lingchong You, and Bernard Mathey-Prevot. "Cyclin D/CDK4/6 activity controls G1 length in mammalian cells.." Plos One 13, no. 1 (2018). https://doi.org/10.1371/journal.pone.0185637.
Wu, Feilun, Jonathan H. Bethke, Meidi Wang, and Lingchong You. "Quantitative and synthetic biology approaches to combat bacterial pathogens.." Current Opinion in Biomedical Engineering 4 (December 2017): 116–26. https://doi.org/10.1016/j.cobme.2017.10.007.
Lopatkin, Allison J., Hannah R. Meredith, Jaydeep K. Srimani, Connor Pfeiffer, Rick Durrett, and Lingchong You. "Persistence and reversal of plasmid-mediated antibiotic resistance.." Nature Communications 8, no. 1 (November 22, 2017). https://doi.org/10.1038/s41467-017-01532-1.
Cao, Yangxiaolu, Yaying Feng, Marc D. Ryser, Kui Zhu, Gregory Herschlag, Changyong Cao, Katherine Marusak, Stefan Zauscher, and Lingchong You. "Programmable assembly of pressure sensors using pattern-forming bacteria.." Nat Biotechnol 35, no. 11 (November 2017): 1087–93. https://doi.org/10.1038/nbt.3978.
Srimani, Jaydeep K., Shuqiang Huang, Allison J. Lopatkin, and Lingchong You. "Drug detoxification dynamics explain the postantibiotic effect.." Molecular Systems Biology 13, no. 10 (October 23, 2017). https://doi.org/10.15252/msb.20177723.
Wu, Feilun, and Lingchong You. "Hacking DNA copy number for circuit engineering.." Nature Genetics 49, no. 8 (July 2017): 1164–65. https://doi.org/10.1038/ng.3923.
Shats, Igor, Michael Deng, Adam Davidovich, Carolyn Zhang, Jungeun S. Kwon, Dinesh Manandhar, Raluca Gordân, Guang Yao, and Lingchong You. "Expression level is a key determinant of E2F1-mediated cell fate.." Cell Death Differ 24, no. 4 (April 2017): 626–37. https://doi.org/10.1038/cdd.2017.12.
Tanouchi, Yu, Anand Pai, Heungwon Park, Shuqiang Huang, Nicolas E. Buchler, and Lingchong You. "Long-term growth data of Escherichia coli at a single-cell level.." Scientific Data 4 (March 28, 2017). https://doi.org/10.1038/sdata.2017.36.
Feng, Y., E. Ngaboyamahina, K. E. Marusak, Y. Cao, L. You, J. T. Glass, and S. Zauscher. "Hybrid (Organic/Inorganic) Electrodes from Bacterially Precipitated CdS for PEC/Storage Applications." Journal of Physical Chemistry C 121, no. 7 (February 23, 2017): 3734–43. https://doi.org/10.1021/acs.jpcc.6b11387.
Cao, Yangxiaolu, Allison Lopatkin, and Lingchong You. "Elements of biological oscillations in time and space.." Nature Structural & Molecular Biology 23, no. 12 (December 2016): 1030–34. https://doi.org/10.1038/nsmb.3320.
Lopatkin, Allison J., Tatyana A. Sysoeva, and Lingchong You. "Dissecting the effects of antibiotics on horizontal gene transfer: Analysis suggests a critical role of selection dynamics.." Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology 38, no. 12 (December 2016): 1283–92. https://doi.org/10.1002/bies.201600133.
Zhang, Carolyn, Ryan Tsoi, Feilun Wu, and Lingchong You. "Processing Oscillatory Signals by Incoherent Feedforward Loops.." Plos Computational Biology 12, no. 9 (September 13, 2016). https://doi.org/10.1371/journal.pcbi.1005101.
Cao, Yangxiaolu, Marc D. Ryser, Stephen Payne, Bochong Li, Christopher V. Rao, and Lingchong You. "Collective Space-Sensing Coordinates Pattern Scaling in Engineered Bacteria.." Cell 165, no. 3 (April 21, 2016): 620–30. https://doi.org/10.1016/j.cell.2016.03.006.
Lopatkin, Allison J., Shuqiang Huang, Robert P. Smith, Jaydeep K. Srimani, Tatyana A. Sysoeva, Sharon Bewick, David K. Karig, and Lingchong You. "Antibiotics as a selective driver for conjugation dynamics.." Nature Microbiology 1, no. 6 (April 11, 2016). https://doi.org/10.1038/nmicrobiol.2016.44.
Zhang, Carolyn, Ryan Tsoi, and Lingchong You. "Addressing biological uncertainties in engineering gene circuits.." Integrative Biology : Quantitative Biosciences From Nano to Macro 8, no. 4 (April 2016): 456–64. https://doi.org/10.1039/c5ib00275c.
Huang, Shuqiang, Anna Jisu Lee, Ryan Tsoi, Feilun Wu, Ying Zhang, Kam W. Leong, and Lingchong You. "Coupling spatial segregation with synthetic circuits to control bacterial survival.." Molecular Systems Biology 12, no. 2 (February 29, 2016). https://doi.org/10.15252/msb.20156567.
Marusak, K. E., Y. Feng, C. F. Eben, S. T. Payne, Y. Cao, L. You, and S. Zauscher. "Cadmium sulphide quantum dots with tunable electronic properties by bacterial precipitation.." Rsc Advances 6, no. 80 (January 2016): 76158–66. https://doi.org/10.1039/C6RA13835G.
Huang, Shuqiang, Jaydeep K. Srimani, Anna J. Lee, Ying Zhang, Allison J. Lopatkin, Kam W. Leong, and Lingchong You. "Dynamic control and quantification of bacterial population dynamics in droplets.." Biomaterials 61 (August 2015): 239–45. https://doi.org/10.1016/j.biomaterials.2015.05.038.
Tanouchi, Yu, Anand Pai, Heungwon Park, Shuqiang Huang, Rumen Stamatov, Nicolas E. Buchler, and Lingchong You. "A noisy linear map underlies oscillations in cell size and gene expression in bacteria.." Nature 523, no. 7560 (July 2015): 357–60. https://doi.org/10.1038/nature14562.
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Lee, Tae J., Jeffrey Wong, Sena Bae, Anna Jisu Lee, Allison Lopatkin, Fan Yuan, and Lingchong You. "A power-law dependence of bacterial invasion on mammalian host receptors.." Plos Computational Biology 11, no. 4 (April 16, 2015). https://doi.org/10.1371/journal.pcbi.1004203.
Meredith, Hannah R., Allison J. Lopatkin, Deverick J. Anderson, and Lingchong You. "Bacterial temporal dynamics enable optimal design of antibiotic treatment.." Plos Comput Biol 11, no. 4 (April 2015). https://doi.org/10.1371/journal.pcbi.1004201.
Meredith, Hannah R., Jaydeep K. Srimani, Anna J. Lee, Allison J. Lopatkin, and Lingchong You. "Collective antibiotic tolerance: mechanisms, dynamics and intervention.." Nature Chemical Biology 11, no. 3 (March 2015): 182–88. https://doi.org/10.1038/nchembio.1754.
Eidum, D., K. Asthana, S. Unni, M. Deng, and L. You. "Construction, visualization, and analysis of biological network models in Dynetica." Quantitative Biology 2, no. 4 (December 1, 2014): 142–50. https://doi.org/10.1007/s40484-014-0036-4.
Srimani, Jaydeep K., and Lingchong You. "Emergent dynamics from quorum eavesdropping.." Chemistry & Biology 21, no. 12 (December 2014): 1601–2. https://doi.org/10.1016/j.chembiol.2014.12.002.
Lopatkin, Allison J., and Lingchong You. "Synthetic biology looks good on paper.." Cell 159, no. 4 (November 2014): 718–20. https://doi.org/10.1016/j.cell.2014.10.003.
Dong, Peng, Manoj V. Maddali, Jaydeep K. Srimani, François Thélot, Joseph R. Nevins, Bernard Mathey-Prevot, and Lingchong You. "Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control.." Nat Commun 5 (September 1, 2014). https://doi.org/10.1038/ncomms5750.
Li, Bochong, and Lingchong You. "Stochastic sensitivity analysis and kernel inference via distributional data.." Biophysical Journal 107, no. 5 (September 2014): 1247–55. https://doi.org/10.1016/j.bpj.2014.07.025.
Tan, Cheemeng, Robert Phillip Smith, Ming-Chi Tsai, Russell Schwartz, and Lingchong You. "Phenotypic signatures arising from unbalanced bacterial growth.." Plos Computational Biology 10, no. 8 (August 7, 2014). https://doi.org/10.1371/journal.pcbi.1003751.
Pai, Anand, Jaydeep K. Srimani, Yu Tanouchi, and Lingchong You. "Generic metric to quantify quorum sensing activation dynamics.." Acs Synthetic Biology 3, no. 4 (April 2014): 220–27. https://doi.org/10.1021/sb400069w.
Lee, Anna Jisu, and Lingchong You. "Cell biology. Cells listen to their inner voice.." Science (New York, N.Y.) 343, no. 6171 (February 2014): 624–25. https://doi.org/10.1126/science.1250244.
Smith, Robert, Cheemeng Tan, Jaydeep K. Srimani, Anand Pai, Katherine A. Riccione, Hao Song, and Lingchong You. "Programmed Allee effect in bacteria causes a tradeoff between population spread and survival.." Proceedings of the National Academy of Sciences of the United States of America 111, no. 5 (February 2014): 1969–74. https://doi.org/10.1073/pnas.1315954111.
Payne, Stephen, and Lingchong You. "Engineered cell-cell communication and its applications.." Advances in Biochemical Engineering/Biotechnology 146 (January 2014): 97–121. https://doi.org/10.1007/10_2013_249.
Srimani, Jaydeep K., Guang Yao, John Neu, Yu Tanouchi, Tae Jun Lee, and Lingchong You. "Linear population allocation by bistable switches in response to transient stimulation.." Plos One 9, no. 8 (January 2014). https://doi.org/10.1371/journal.pone.0105408.
Payne, Stephen, Bochong Li, Yangxiaolu Cao, David Schaeffer, Marc D. Ryser, and Lingchong You. "Temporal control of self-organized pattern formation without morphogen gradients in bacteria.." Mol Syst Biol 9 (October 8, 2013). https://doi.org/10.1038/msb.2013.55.
Shats, Igor, Michael L. Gatza, Beiyu Liu, Steven P. Angus, Lingchong You, and Joseph R. Nevins. "FOXO transcription factors control E2F1 transcriptional specificity and apoptotic function.." Cancer Research 73, no. 19 (October 2013): 6056–67. https://doi.org/10.1158/0008-5472.CAN-13-0453.
Smith, R. P., Y. Tanouchi, and L. You. "Synthetic Microbial Consortia and their Applications," August 27, 2013, 243–58. https://doi.org/10.1016/B978-0-12-394430-6.00013-3.
Li, B., and L. You. "Predictive power of cell-to-cell variability." Quantitative Biology 1, no. 2 (June 1, 2013): 131–39. https://doi.org/10.1007/s40484-013-0013-3.
Tanouchi, Yu, Anna Jisu Lee, Hannah Meredith, and Lingchong You. "Programmed cell death in bacteria and implications for antibiotic therapy.." Trends in Microbiology 21, no. 6 (June 2013): 265–70. https://doi.org/10.1016/j.tim.2013.04.001.
Zhang, Ying, Yi-Ping Ho, Ya-Ling Chiu, Hon Fai Chan, Ben Chlebina, Tom Schuhmann, Lingchong You, and Kam W. Leong. "A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.." Biomaterials 34, no. 19 (June 2013): 4564–72. https://doi.org/10.1016/j.biomaterials.2013.03.002.
Pai, Anand, Yu Tanouchi, and Lingchong You. "Optimality and robustness in quorum sensing (QS)-mediated regulation of a costly public good enzyme.." Proceedings of the National Academy of Sciences of the United States of America 109, no. 48 (November 9, 2012): 19810–15. https://doi.org/10.1073/pnas.1211072109.
Tanouchi, Yu, Robert P. Smith, and Lingchong You. "Engineering microbial systems to explore ecological and evolutionary dynamics.." Current Opinion in Biotechnology 23, no. 5 (October 2012): 791–97. https://doi.org/10.1016/j.copbio.2012.01.006.
Riccione, Katherine A., Robert P. Smith, Anna J. Lee, and Lingchong You. "A synthetic biology approach to understanding cellular information processing.." Acs Synthetic Biology 1, no. 9 (September 2012): 389–402. https://doi.org/10.1021/sb300044r.
Chin, Jason W., and Lingchong You. "A different life?." Current Opinion in Chemical Biology 16, no. 3–4 (August 2012): 243–44. https://doi.org/10.1016/j.cbpa.2012.07.009.
Stone, Jana E., Philip N. Benfey, and Lingchong You. "Promoting collaborative interdisciplinary research at the Duke Center for Systems Biology.." Acs Synthetic Biology 1, no. 5 (May 2012): 153–55. https://doi.org/10.1021/sb300036z.
Payne, Stephen, Robert Phillip Smith, and Lingchong You. "Quantitative analysis of the spatiotemporal dynamics of a synthetic predator-prey ecosystem.." Methods in Molecular Biology (Clifton, N.J.) 813 (January 2012): 315–30. https://doi.org/10.1007/978-1-61779-412-4_19.
Song, Hao, and Lingchong You. "Modeling spatiotemporal dynamics of bacterial populations.." Methods in Molecular Biology (Clifton, N.J.) 880 (January 2012): 243–54. https://doi.org/10.1007/978-1-61779-833-7_11.
Tan, Cheemeng, Robert Phillip Smith, Jaydeep K. Srimani, Katherine A. Riccione, Sameer Prasada, Meta Kuehn, and Lingchong You. "The inoculum effect and band-pass bacterial response to periodic antibiotic treatment.." Mol Syst Biol 8 (2012). https://doi.org/10.1038/msb.2012.49.
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Tanouchi, Yu, Anand Pai, Nicolas E. Buchler, and Lingchong You. "Programming stress-induced altruistic death in engineered bacteria.." Molecular Systems Biology 8 (January 2012). https://doi.org/10.1038/msb.2012.57.
Wong, Jeffrey V., Bochong Li, and Lingchong You. "Tension and robustness in multitasking cellular networks.." Plos Computational Biology 8, no. 4 (January 2012). https://doi.org/10.1371/journal.pcbi.1002491.
Bonassi, Fernando V., Lingchong You, and Mike West. "Bayesian learning from marginal data in bionetwork models.." Statistical Applications in Genetics and Molecular Biology 10, no. 1 (October 27, 2011). https://doi.org/10.2202/1544-6115.1684.
Hallen, Mark, Bochong Li, Yu Tanouchi, Cheemeng Tan, Mike West, and Lingchong You. "Computation of steady-state probability distributions in stochastic models of cellular networks.." Plos Computational Biology 7, no. 10 (October 13, 2011). https://doi.org/10.1371/journal.pcbi.1002209.
Wong, Jeffrey V., Peng Dong, Joseph R. Nevins, Bernard Mathey-Prevot, and Lingchong You. "Network calisthenics: control of E2F dynamics in cell cycle entry.." Cell Cycle 10, no. 18 (September 15, 2011): 3086–94. https://doi.org/10.4161/cc.10.18.17350.
Song, Hao, Stephen Payne, Cheemeng Tan, and Lingchong You. "Programming microbial population dynamics by engineered cell-cell communication.." Biotechnology Journal 6, no. 7 (July 2011): 837–49. https://doi.org/10.1002/biot.201100132.
Elliott, Nelita, Tae Lee, Lingchong You, and Fan Yuan. "Proliferation behavior of E. coli in a three-dimensional in vitro tumor model.." Integrative Biology : Quantitative Biosciences From Nano to Macro 3, no. 6 (June 2011): 696–705. https://doi.org/10.1039/c0ib00137f.
Wong, J. V., G. Yao, J. R. Nevins, and L. You. "Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells." Methods in Enzymology 497 (May 30, 2011): 221–37. https://doi.org/10.1016/B978-0-12-385075-100010-X.
Yao, Guang, Cheemeng Tan, Mike West, Joseph R. Nevins, and Lingchong You. "Origin of bistability underlying mammalian cell cycle entry.." Molecular Systems Biology 7 (April 2011). https://doi.org/10.1038/msb.2011.19.
Wong, Jeffrey V., Guang Yao, Joseph R. Nevins, and Lingchong You. "Viral-mediated noisy gene expression reveals biphasic E2f1 response to MYC.." Molecular Cell 41, no. 3 (February 2011): 275–85. https://doi.org/10.1016/j.molcel.2011.01.014.
Li, Bochong, and Lingchong You. "Synthetic biology: Division of logic labour.." Nature 469, no. 7329 (January 2011): 171–72. https://doi.org/10.1038/469171a.
Wong, Jeffrey V., Guang Yao, Joseph R. Nevins, and Lingchong You. "Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells.." Methods in Enzymology 497 (January 2011): 221–37. https://doi.org/10.1016/b978-0-12-385075-1.00010-x.
Lee, Tae J., Guang Yao, Dorothy C. Bennett, Joseph R. Nevins, and Lingchong You. "Stochastic E2F activation and reconciliation of phenomenological cell-cycle models.." Plos Biology 8, no. 9 (September 21, 2010). https://doi.org/10.1371/journal.pbio.1000488.
Marguet, Philippe, Yu Tanouchi, Eric Spitz, Cameron Smith, and Lingchong You. "Oscillations by minimal bacterial suicide circuits reveal hidden facets of host-circuit physiology.." Plos One 5, no. 7 (January 2010). https://doi.org/10.1371/journal.pone.0011909.
Wang, Quanli, Jarad Niemi, Chee-Meng Tan, Lingchong You, and Mike West. "Image segmentation and dynamic lineage analysis in single-cell fluorescence microscopy.." Cytometry. Part a : The Journal of the International Society for Analytical Cytology 77, no. 1 (January 2010): 101–10. https://doi.org/10.1002/cyto.a.20812.
Song, Hao, Stephen Payne, Meagan Gray, and Lingchong You. "Spatiotemporal modulation of biodiversity in a synthetic chemical-mediated ecosystem.." Nature Chemical Biology 5, no. 12 (December 2009): 929–35. https://doi.org/10.1038/nchembio.244.
Tan, Cheemeng, Philippe Marguet, and Lingchong You. "Emergent bistability by a growth-modulating positive feedback circuit.." Nature Chemical Biology 5, no. 11 (November 2009): 842–48. https://doi.org/10.1038/nchembio.218.
Pai, Anand, Yu Tanouchi, Cynthia H. Collins, and Lingchong You. "Engineering multicellular systems by cell-cell communication.." Current Opinion in Biotechnology 20, no. 4 (August 2009): 461–70. https://doi.org/10.1016/j.copbio.2009.08.006.
Tanouchi, Yu, Anand Pai, and Lingchong You. "Decoding biological principles using gene circuits.." Molecular Biosystems 5, no. 7 (July 2009): 695–703. https://doi.org/10.1039/b901584c.
Brenner, K., L. You, and F. H. Arnold. "Response to Goldman and Brown: Making sense of microbial consortia using ecology and evolution." Trends in Biotechnology 27, no. 1 (January 1, 2009). https://doi.org/10.1016/j.tibtech.2008.10.004.
Isaacs, Farren J., and Lingchong You. "A brave new synthetic world. A report of the first meeting on 'Frontiers in Synthetic Biology', Boston, USA, 1-2 December 2008.." Genome Biology 10, no. 2 (January 2009). https://doi.org/10.1186/gb-2009-10-2-302.
Pai, Anand, and Lingchong You. "Optimal tuning of bacterial sensing potential.." Molecular Systems Biology 5 (January 2009). https://doi.org/10.1038/msb.2009.43.
Song, H., M. Gray, and L. You. "Spatiotemporal modulation of biodiversity in a synthetic predator-prey ecosystem." Aiche Annual Meeting, Conference Proceedings, December 1, 2008.
Smith, Cameron, Hao Song, and Lingchong You. "Signal discrimination by differential regulation of protein stability in quorum sensing.." Journal of Molecular Biology 382, no. 5 (October 2008): 1290–97. https://doi.org/10.1016/j.jmb.2008.08.009.
Brenner, Katie, Lingchong You, and Frances H. Arnold. "Engineering microbial consortia: a new frontier in synthetic biology.." Trends in Biotechnology 26, no. 9 (September 2008): 483–89. https://doi.org/10.1016/j.tibtech.2008.05.004.
Tanouchi, Yu, Dennis Tu, Jungsang Kim, and Lingchong You. "Noise reduction by diffusional dissipation in a minimal quorum sensing motif.." Plos Comput Biol 4, no. 8 (August 29, 2008). https://doi.org/10.1371/journal.pcbi.1000167.
Yao, Guang, Tae Jun Lee, Seiichi Mori, Joseph R. Nevins, and Lingchong You. "A bistable Rb-E2F switch underlies the restriction point.." Nature Cell Biology 10, no. 4 (April 2008): 476–82. https://doi.org/10.1038/ncb1711.
Lee, Tae, Guang Yao, Joseph Nevins, and Lingchong You. "Sensing and integration of Erk and PI3K signals by Myc.." Plos Computational Biology 4, no. 2 (February 29, 2008). https://doi.org/10.1371/journal.pcbi.1000013.
Balagaddé, Frederick K., Hao Song, Jun Ozaki, Cynthia H. Collins, Matthew Barnet, Frances H. Arnold, Stephen R. Quake, and Lingchong You. "A synthetic Escherichia coli predator-prey ecosystem.." Molecular Systems Biology 4 (January 2008). https://doi.org/10.1038/msb.2008.24.
Wong, Jeffrey V., Hao Song, and Lingchong You. "A whole more than the sum of its synthetic parts.." Acs Chemical Biology 3, no. 1 (January 2008): 27–29. https://doi.org/10.1021/cb700256w.
Tan, Cheemeng, Faisal Reza, and Lingchong You. "Noise-limited frequency signal transmission in gene circuits.." Biophysical Journal 93, no. 11 (December 2007): 3753–61. https://doi.org/10.1529/biophysj.107.110403.
Reza, F., K. Chandran, M. Feltz, A. Heinz, E. Josephs, P. O'Brien, B. Van Dyke, et al. "Engineering novel synthetic biological systems." Iet Synthetic Biology 1, no. 1–2 (August 15, 2007): 48–52. https://doi.org/10.1049/iet-stb:20060004.
You, L. "Engineering synthetic killer circuits in bacteria." Proceedings Design Automation Conference, August 2, 2007, 636–37. https://doi.org/10.1109/DAC.2007.375242.
Marguet, Philippe, Frederick Balagadde, Cheemeng Tan, and Lingchong You. "Biology by design: reduction and synthesis of cellular components and behaviour.." Journal of the Royal Society, Interface 4, no. 15 (August 2007): 607–23. https://doi.org/10.1098/rsif.2006.0206.
Tan, Cheemeng, Hao Song, Jarad Niemi, and Lingchong You. "A synthetic biology challenge: making cells compute.." Molecular Biosystems 3, no. 5 (May 2007): 343–53. https://doi.org/10.1039/b618473c.
Song, Hao, and Lingchong You. "Evolving sensitivity.." Acs Chemical Biology 1, no. 11 (December 2006): 681–82. https://doi.org/10.1021/cb6004596.
You, L., and J. Yin. "Evolutionary design on a budget: robustness and optimality of bacteriophage T7.." Systems Biology 153, no. 2 (March 2006): 46–52. https://doi.org/10.1049/ip-syb:20050026.
Ozaki, J., M. Barnet, C. H. Collins, F. H. Arnold, and L. You. "A synthetic E.Coli predator-prey system." Aiche Annual Meeting, Conference Proceedings, December 1, 2005.
Balagaddé, Frederick K., Lingchong You, Carl L. Hansen, Frances H. Arnold, and Stephen R. Quake. "Long-term monitoring of bacteria undergoing programmed population control in a microchemostat.." Science (New York, N.Y.) 309, no. 5731 (July 2005): 137–40. https://doi.org/10.1126/science.1109173.
You, Lingchong, Robert Sidney Cox, Ron Weiss, and Frances H. Arnold. "Programmed population control by cell-cell communication and regulated killing.." Nature 428, no. 6985 (April 4, 2004): 868–71. https://doi.org/10.1038/nature02491.
You, Lingchong. "Toward computational systems biology.." Cell Biochemistry and Biophysics 40, no. 2 (January 2004): 167–84. https://doi.org/10.1385/cbb:40:2:167.
You, Lingchong, Apirak Hoonlor, and John Yin. "Modeling biological systems using Dynetica--a simulator of dynamic networks.." Bioinformatics (Oxford, England) 19, no. 3 (February 2003): 435–36. https://doi.org/10.1093/bioinformatics/btg009.
Srivastava, R., L. You, J. Summers, and J. Yin. "Stochastic vs. deterministic modeling of intracellular viral kinetics.." Journal of Theoretical Biology 218, no. 3 (October 2002): 309–21. https://doi.org/10.1006/jtbi.2002.3078.
You, Lingchong, Patrick F. Suthers, and John Yin. "Effects of Escherichia coli physiology on growth of phage T7 in vivo and in silico.." Journal of Bacteriology 184, no. 7 (April 2002): 1888–94. https://doi.org/10.1128/JB.184.7.1888-1894.2002.
You, Lingchong, and John Yin. "Dependence of epistasis on environment and mutation severity as revealed by in silico mutagenesis of phage t7.." Genetics 160, no. 4 (April 2002): 1273–81.
You, L., and J. Yin. "Simulating the growth of viruses.." Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing, January 2001, 532–43. https://doi.org/10.1142/9789814447362_0051.
You, L., and J. Yin. "Patterns of regulation from mRNA and protein time series.." Metabolic Engineering 2, no. 3 (July 2000): 210–17. https://doi.org/10.1006/mben.1999.0139.
Endy, D., L. You, J. Yin, and I. J. Molineux. "Computation, prediction, and experimental tests of fitness for bacteriophage T7 mutants with permuted genomes.." Proceedings of the National Academy of Sciences of the United States of America 97, no. 10 (May 2000): 5375–80. https://doi.org/10.1073/pnas.090101397.
You, L., and J. Yin. "Amplification and spread of viruses in a growing plaque.." Journal of Theoretical Biology 200, no. 4 (October 1999): 365–73. https://doi.org/10.1006/jtbi.1999.1001.
Endy, D., L. You, I. J. Molineux, and J. Yin. "Prediction, design, and characterization of alternate genetic element orders for bacteriophage T7.." Abstracts of Papers of the American Chemical Society 217 (March 1999): U205–U205.
You, L., Q. Liu, Y. Shi, C. X. Wang, M. Lahaye, and V. Tran. "The conformational study of β-D-GlcA-(1,4) -L-Rha in solution by NMR and molecular dynamics simulations." Chemical Physics 224, no. 1 (November 15, 1997): 81–94. https://doi.org/10.1016/S0301-0104(97)00246-2.
Sysoeva, Tatyana A., Youlim Kim, and Lingchong You. "REGULATION OF CONJUGATIVE TRANSFER OF beta-LACTAM RESISTANCE FROM UROPATHOGENIC STRAINS OF ESCHERICHIA COLI." In Journal of Urology, 199:E285–E285. ELSEVIER SCIENCE INC, 2018.
Sysoeva, Tatyana, and Lingchong You. "REGULATION OF CONJUGATIVE TRANSFER OF B-LACTAM RESISTANCE FROM UROPATHOGENIC STRAINS OF ESCHERICHIA COLI." In Neurourology and Urodynamics, 37:S529–30. WILEY, 2018.
Teaching & Mentoring
BME 574: Modeling and Engineering Gene Circuits (GE, MC) 2020
CBB 574: Modeling and Engineering Gene Circuits (GE, MC) 2020
EGR 393: Research Projects in Engineering 2020
MGM 293: Research Independent Study I 2020
MGM 593: Research Independent Study 2020
PHARM 394: Research Independent Study 2020
BME 260L: Modeling Cellular and Molecular Systems 2019
BME 792: Continuation of Graduate Independent Study 2019
BME 493: Projects in Biomedical Engineering (GE) 2018
BME 590: Special Topics in Biomedical Engineering 2018
BME 791: Graduate Independent Study 2018
COMPSCI 394: Research Independent Study 2018
Scholarly, Clinical, & Service Activities
Outreach & Engaged Scholarship
Bass Connections Faculty Team Member. Blue Devil Resistome Project. August 2017 - May 2018 2017 - 2018
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Hey Rex That's interesting, and an idea I hadn't considered. We've replaced single injectors many times over the years and never had an issue with fuel control. But I can see that Ford would try to do that since there is no way to monitor fuel control with an ... Login to read more.


A Focus on Student Art Tours of New York City and Washington D.C.
Artists have been challenging us to see our world in new ways for centuries. Art teachers may want to consider bringing classes on tour of museums in New York City and Washington DC that house the most comprehensive collections of art in the United States. When organizing student tours of these cities it is important to consider these museums. It is no exaggeration to say that lives can be changed for the better by visiting such collections of art. For many students, this may be their only visit to a museum in a large U.S. city. Other students may decide to live in a large city and experience the cultural life firsthand.
The Guggenheim Museum, the Metropolitan Museum of Art, and the Museum of Modern Art are the three highlighted museums for New York City. The Phillips Collection, the National Portrait Gallery, and the Hirshhorn Museum & Sculpture Garden are recommended stops on the itinerary for Washington DC.
On Fifth Avenue in New York City the Solomon R. Guggenheim Museum is one of the most recognizable and iconic buildings. The Guggenheim Museum was designed by the American architect Frank Lloyd Wright. When planning your class trip to New York City, please look at the museum's online events calendar. Not only are there daily tours of "Art in the Round" but also there are films, and often in-depth discussions on pieces in the collection and even on the building itself. While the Guggenheim houses the private collections of art there are always new acquisitions on view. Check the timing of a student trip and coordinate attendance of special shows at the Guggenheim with a student travel company representative.
Not far from the Guggenheim is the Metropolitan Museum of Art, boasting one of the largest, most eclectic collections of art in the world. This world-class structure houses paintings, arms and armor, furnishings, sculpture and design from every imaginable worldwide location. This is an enormous museum, so please be sure to see their website to plan your visit with specific concentrations on the permanent collection as well as to view current and upcoming traveling exhibitions.
The Museum of Modern Art, known the world over as MoMA, has the most important collection of some of the most astonishing talent the world has ever seen. The permanent collection contains some of the most sought after art by Matisse, Van Gogh, Balla, Rothko, Warhol and others. One of the most popular exhibitions which is on view until January, 2016 is called, "This Is for Everyone: Design Experiments for the Common Good." The future of design and the technology that is creating the road to that future are highlighted in this strange and beautiful exhibition.
In the heart of Washington D.C., student travel groups may visit the National Gallery of Art and the famous Phillips Collection, as well as the National Portrait Gallery and the Hirshhorn Museum and Sculpture Garden.
The National Gallery of Art features painting, sculptures, prints, drawings and decorative arts from the Renaissance to the present day. The Photographic collection dates from 1839. On a pleasant day, student tour groups can wander through the Sculpture Garden. Here students can see art from around the world and the work of artists who either studied or taught at the Corcoran College of Art and Design and went on to distinguish themselves. A Media Arts section is also part of the museum, representing contemporary artists that work in digital media.
Art lovers from around the world travel to the Phillips Collection each year to see the Mark Rothko Room, and other famous paintings like Renoir's classic "Boating Party." Georgia O'Keefe, Man Ray and other artists are part of the permanent collection. Paul Klee fans know about the large number of his paintings at the Phillips, and visit often. The museum's website has comprehensive educational resources for much of the catalog. For instance Jacob Lawrence's "The Migration Series" is featured on the website. This series of sixty paintings depicts the migration of African Americans from the South to the North in the early twentieth century.
The National Portrait Gallery houses a complete collection of original portraits of the presidents of the United States. Besides presidents, the gallery holds paintings of other great American leaders such as Dr. Martin Luther King and Rosa Parks. Pop culture Hollywood icons Marilyn Monroe and Woody Allen share space with musical giants like George and Ira Gershwin. A visit to the National Portrait Gallery is as much about art as it is history. There are special exhibits detailing trends in America as well as struggles. See portraits of activists for labor rights, civil rights, and Native American activist Leonard Crow Dog, among many other famous Americans.
The Hirshhorn Museum and Sculpture Garden has a staff dedicated to a deep understanding of art and artist. The museum mission includes the statement, "Good art should elicit a response of 'Huh? Wow!' as opposed to 'Wow! Huh?'" Paintings by Francis Bacon and Joan Mitchell are part of this historic collection. The Sculpture Garden houses some of the most innovative and famous sculptors from around the world, including Brancusi, Rodin, Miro and others. Student tour groups are encouraged to visit the Hirshhorn and learn more with ARTLAB+ and special comprehensive class tours of the collections.
These museums house some of the great treasures of the United States, and visiting them with art classes is part of seeing art as a unique educational tool and framework for not only historical record, but also as a means to forge a better future for humanity and the planet. Artists are the best collaborators a teacher can have. Consider art the classroom itself. Take student groups on tour of these museums to expand their knowledge of art throughout the ages.
For more information about student art tours of New York City, Washington D.C. and other U.S. destinations, visit http://www.educatioanltravelconsultants.com.


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I attended Georgina's activate your sixth sense workshop last Saturday & would like to say what an amazing day it was.  Georgina is a very gifted lady with a wealth of knowledge, her life story is truly remarkable & I could have sat there all night & listened to her journey & the many instances that spirit had intervened in her life.  The exercises we did really assisted us with establishing what our primary "clair" was & there were lots of aha moments from the other attendees of the workshop.  I found the workshop extremely informative, I loved all the psychic protection information & will use the techniques on a daily basis.   Georgina also explained to us how to ask spirit for answers during our dreamtime,  I actually did this a couple of nights ago & it worked beautifully!  At the end of the workshop we were all treated to a psychometry reading, I could totally relate to my reading it all made perfect sense to me.  Georgina mentioned there was an energy drain related to my kidneys, when I visited my chiropractor (who also practices kinesiology) a couple of days after the workshop he confirmed via kinesiology this was correct, there was a blockage in my kidney meridian.   Looking forward to booking in for a futuristic reading in the very near future & attending the next workshop.  I would highly recommend booking in for a reading with Georgina & attending any future workshops that Georgina runs.  Thank you for sharing you wealth of knowledge Georgina & for being you.


Here are some of the best free apps, app updates and new apps that have landed in the App Store recently. Today's notable apps include a 50% discount on PDF Expert 5, the exciting new Enlight photo editor, and a feature-filled update to Hype 3. All app prices are USD and subject to change. Some deals may expire quickly, so grab them while you can.
Plump [Now free, down from $0.99] Plump is a tiny and fat creature who loves chocolate! Help Plump eat chocolates in a wonderful land full of adventures!
my monster voice [Now free, down from $0.99] Ever wanted to sound like a monster? 'my monster voice' changes your voice into creepy or funny monster sounds.
Touch™ [Now free, down from $0.99] Touch redefines how we stay in touch with close friends and family.
FunSlides - Make HD video from photos [Now free, down from $1.99] With FunSlides you can create fantastic REALTIME, HD video slideshows from your favorite photos.
C.H.A.O.S Combat Copters HD - №1 Multiplayer Helicopter Simulator [Now free, down from $2.99] CHAOS - is a unique opportunity to act online as a pilot of a military helicopter.
NanoStudio [On sale for $6.99, down from $9.99] NanoStudio is a recording studio for iOS, OS X and Windows.
Mix & Mash [On sale for $0.99, down from $1.99] Looking for an awesome crossfade app to improve your music? Look no further - Mix & Mash is simply the best crossfade app for iOS!
Osmos [On sale for $0.99, down from $2.99] Osmos features unique physics-based play, stellar graphics, and a hypnotic ambient soundtrack. Absorb or be absorbed!
Osmos for iPad [On sale for $0.99, down from $4.99] Osmos features unique physics-based play, stellar graphics, and a hypnotic ambient soundtrack. Absorb or be absorbed!
PDF Expert 5 - Fill forms, annotate PDFs, sign documents [On sale for $9.99, down from $4.99] PDF Expert 5 is a must-have app for anyone who reads, annotates or edits PDF documents on iPad or iPhone.
Auro: A Monster-Bumping Adventure [On sale for $1.99, down from $2.99] Set in an original fantasy universe, guide the brash, spoiled Prince Auro through procedurally generated dungeons, with only a handful of tactical spells - and your wits - to protect you.
WiFi Scanner [On sale for $6.99, down from $9.99] Using WiFi Scanner you'll see nearby wireless access points and their details, including channels, signal levels (RSSI), noise, channel width, and MAC address, signal quality, maximum data rate, encryption, and much more.
Seasonality Core [On sale for $14.99, down from $24.99] Seasonality Core has been the premier app for weather enthusiasts for the past 10 years.
Arizona Rose and the Pirates' Riddles [Now Free, down from $6.99] A clever code holds the key to buried treasure!
Total War: SHOGUN 2 Collection [On sale for $24.99, down from $49.99] Master your clan's unique martial strengths to triumph in spectacular real-time battles and dominate the turn-based campaign map.
Blink: Better Affiliate Links [$4.99] Blink is everywhere you need it. Blink has a powerful extension that makes its search and link conversion functionality available from other apps, so you can create links without interrupting the flow of your work. Blink also has URL scheme support, so you can incorporate it into workflows with other apps that support URL schemes.
Woodpost by Fuzel - Print your photos on wood [Free] Everything in the app, from the beautifully curated photo templates, to the simple & straightforward ordering process brings you the best way to print photos on wood.
Enlight [$3.99] Download Enlight - every photo app you've ever needed, wanted, or dreamed of - combined into one powerful, beautifully designed photo editor.
Introducing the new signing extension for iOS8 which enables you to complete and send your paperwork right away from their favourite personal and business apps such as Dropbox, Box, Cloudmagic, Mailbox, Onedrive, Camscanner, GeniusScan, Scannable, Slack and hundreds of other apps.
Now you can simply open a document sign and send it without the usual back and forth switching between apps.
The Hype Pro in-app purchase adds powerful features to Hype 3, such as Persistent Symbols, Dark interface theme and more.


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The custodial parent is the person who has primary care, custody and control of a minor child(ren). A custodian may also be a caretaker who has legal custody and/or guardianship of the child(ren).
Below you will find information for custodial parents.
Application for Child Support Services: If you are not receiving public assistance, you can apply for child support services by contacting any Division of Child Support office or by calling 605.773.3641. You will have to fill out an application and pay a $5 fee.
Voluntary Paternity Acknowledgment: To voluntary acknowledge paternity, both parents must sign a paternity affidavit under oath. By signing the affidavit, the law presumes the named father is the biological father of the child and action can proceed to establish a child support obligation without any further legal proceeding to establish paternity.
Child Support Obligation: The South Dakota Legislature has established guidelines which courts must use to determine child support payments.
Medical Support: DCS will only enforce health insurance if the noncustodial parent is ordered to provide and available through employment.
Modification of Child Support: If you have a South Dakota child support order and want to modify the child support obligation, a Petition for Modification is required to be completed to initiate the process.
Automated Voice Response System: Custodial parents can use the automated voice response system toll-free from a touchtone phone to obtain up-to-date information about their child support payments.
DCS Customer Connect: Custodial parents can access a secure, interactive website to obtain recent payment information, amount of child support owed, and other beneficial information.
Electronic Payment of Child Support: Custodial parents can have their child support payments deposited directly into their bank accounts or an electronic payment called Way2Go MasterCard.
Conduent Way2Go Card: Custodial parents who have opted for the Way2Go MasterCard are able to access account transactions.
Child Support Offices
Change of Address and/or Employer
Child Support Calculator and Worksheet
Conduent Way2Go Card

redpajama_set_name: RedPajamaCommonCrawl


Moist sponge and a core of chocolate ice cream, finished with a gooey caramel sauce? Yes please!
Typical energy values per 100g: 1289kJ/308kcal.
Preheat the oven to 200C/180C Fan/Gas 6. Butter the insides of the pudding basins and line the base of each with baking paper. Put a baking tray in the oven to heat up.
Put the butter and dark chocolate in a bowl over a pan of simmering water and allow to melt. Remove the bowl from the pan, stir and set aside to cool for 15 mins.
Put the eggs, the yolks and the sugar in a bowl. Beat with an electric whisk for 5-10 mins until thick, pale and fluffy. Gently fold in the chocolate mixture, then fold in the flour.
Divide between the pudding basins then put on the heated baking tray and bake for 12 mins. The tops should be crisp but the centres should still be slightly gooey.
Scoop out the centres of the puddings, then place a ball of ice cream into each one. Use the scooped-out middles to cover the ice cream, flattening the surface with a small palette knife or blunt-bladed table knife.
Turn out each pudding onto a serving plate and remove the baking paper from the top. Warm the salted caramel sauce gently in a small pan, then pour a little over each pudding. Serve immediately.


MySQL server version for the right syntax to use near '@hotmail.com)' at line 1"
I will really apprecite any help here.
also, you are leaving yourself open to sql injection unless your server has magic quotes turned on. you can fix that with running $email_ad through addslashes() before putting it into the sql query.


text:
Jose Lorenzo praises ONYX control platform on Giovanni Pernice "This is Me" 2022 tour
Lighting designer, director and operator Jose Lorenzo uses Obsidian Control Systems' ONYX lighting control platform on tours in the UK and ran a 78 show tour by "Strictly Come Dancing" champion Giovanni Pernice using an NX2™ lighting console. The show, "This is Me," toured the UK and Ireland and highlighted Pernice's unique showmanship supported by a cast of dancers and singers.
Lorenzo initially purchased an NX Wing™, the complementary USB control surface to the ONYX platform, and used it on some local tours in the UK. Later in 2021, he bought Obsidian's compact yet full‐featured NX2 lighting console to use on the 2022 "This is Me" tour. "The number of parameters you get out of the console without having to spend a lot of money on NPU's and computer processor units is fantastic," he stated.
"We live in a world now where some lighting fixtures can take up to 200 channels so having more parameters is important and is one of the strengths of ONYX." The NX2 is able to process 64 Universes right inside the console without the need for costly external processing networks.
On "This is Me," Lorenzo controlled up to 80 intelligent lighting fixtures, some pixel mapped, some not. He ran up to 16 Universes on the show, 4 straight out of the back of the NX2 desk and 12 out of a NETRON EN12™, a 12‐port Ethernet to DMX gateway. A pair of 10‐ port NETRON DMX 10‐5™ DMX splitters were also used for data distribution. He also hooked into and controlled the house lights at each venue. "Networking with the Obsidian series products is really easy and straight forward compared to piecing together a system using different brands," he said. "It's also nice to have a console where you don't need to block off a bunch of seats to fit it in a house."
obsidiancontrol.com
elationlighting.com


While women make up about half the workforce, they remain underrepresented in boardrooms and C-Suites. This despite the fact that organizations with greater gender parity outperform others, and female leaders are better than their male counterparts at skills that can result in increased employee engagement and retention.
To improve your leadership development program to meet the needs of women employees with leadership potential, download Storming the C-Suite: Developing Your Female Leaders.
The natural traits that make women successful leaders.
Why organizations with mostly male leadership teams could be missing out on top talent.
Simple organizational changes you can make to support potential leaders.
Be sure to contact the corporate training experts at TrainingFolks for assistance in implement or updating your organization's leadership development program.


What a beautiful colour! It's amazing those tubers are still growing after all this time, she is obviously green fingered. Nice to see another peony this week.
Beautiful colour. It always amazes me how tight the buds on a peony are but how big the blooms are. What a shame they only last a couple of days.
A very late catch up from #HDYGG which did seem to be dominated by lovely peonies - I think this one was my favourite colour though.


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text:
Educator Course Catalog
Courses on Demand
Independent Learners
Experiential Learning Project
ExperienceED
Experiential Learning Artifacts
ELP Artifacts: American Government
ELP Artifacts: Introduction to Communication
ELP Artifacts: Introduction to Information Technology
TEL Mastery Standards
About TEL
The Four P's of Sustainable College-Level Learning
Nov 10, 2019 | Learning Design
As the leader of an organization committed to affordable learning solutions, and as the architect behind TEL's $99 courses for college credit, I field plenty of questions about how to design and deliver affordable education in a sustainable and scalable fashion.
How can you offer courses at such low prices when even community colleges and regional public institutions charge 4X to 5X as much (plus textbooks and fees I many cases)?
How can you provide high-quality content and engaging learning experiences with such low prices?
Design Constraints and Assumptions
The answers to these questions begin with a discussion about constraints and assumptions.
From the beginning, TEL has utilized a backward design model to define our products and pricing, which has resulted in four foundational constraints that we impose on our work.
Affordability — TEL is a missional organization driven by a commitment to make quality learning affordable for and accessible by everyone. Early on, we determined through conversation with young adults and adults that we would need to provide three-credit-hour college courses for between $75-$100 in order to give anyone in the U.S. the opportunity to get started with their postsecondary learning.
Quality — A second constraint on our work is related to providing a quality learning experience. At TEL, we define this quality learning experience as one that leads to the demonstrable mastery of course concepts (and tied to granular learning outcomes). In other words, our courses must provide measurable evidence that students have learned course subject matter and that they can apply their learning in meaningful ways.
Flexibility — We also understand that the impact of our work is, in many ways, determined by the number of different audiences our products can reach. In order to meet the needs of diverse markets, we must design courses that easily adaptable to a wide variety of implementation models, audiences, and schedules. They must support independent and self-paced learners — from working adults to homeschool students — looking for a flexible solution to earn college credit. They must work for urban school districts and rural schools, homeschool and independent learners, and medium-sized businesses and correctional centers.
Accessibility — Equally important, TEL courses must be widely accessible if we are to achieve our mission. This means course materials should meet the needs of resource-constrained schools, students with low-bandwidth availability, and learners with special needs. For TEL, accessibility also extends to reaching learners with personal or social mindsets that likely prevent them from enrolling in traditional college courses — first-generation college students, adult workers who have been out of school for 10+ years, and incarcerated men and women.
The Four P's of Designing for Affordable, Sustainable College-Level Learning
We have worked, for more than two years now, to design college-level courses that can help us reach our goal of creating a model for affordable, sustainable college learning that can make it possible for anyone in the U.S, to start or continue their post-secondary studies. Our backward design approach — starting with final product design and constraints, and then working backward to define processes and product to match — has made it possible for us to work intentionally and relatively quickly to meet our mission goals. Along the way, we have identified four distinct areas or opportunities for innovation to support our efforts.
At TEL, process innovation applies to organizational structure, communication protocols and tools, workflows, and logistics. In everything we do, we strive to find ways to be cost-effective, efficient, and effective. This affects decisions about hiring experts vs. training people internally, as well as finding the right balance for both. It influences company culture, decisions about the tools we use, and what we look for in employees and contractors. It also means continuous process engineering, often resulting in frequent and quick changes. Of course, our emphasis on the process only works if everyone in the feels empowered to test and implement changes that will lead to our desired results.
While our end product is education via the delivery of online/hybrid courses/services, much of what we do resembles the work of traditional education publishers. We design, create, publish, and deliver content in the form of college-level courses. Stages of development include learning design (course design, content outline, assessment-to-skills alignment), content curation and creation, editorial, media development, production, QA, and final product publishing for use by students. Part of our innovation is tied to the utilization of a single product model that supports our learning goals and requirements for rapid publishing and revision processes. This innovative product model allows us to integrate everything from product development and production to dynamic customization to our partner institutions/schools and efficient course redesign within one framework with complementary workflows.
Our products at TEL are a combination of learning design, content, instruction, and technology. We spend significant time and energy determining the right amount and use of each of these elements. We use learning design to address learning outcomes and experience and to integrate different levels of instruction into our content. Our content model supports our goals for student mastery as well as cost-effective and flexible production via templated, stackable lessons. To complement these efforts, our technology is designed specifically to support scalable affordability, both in terms of publishing and instruction. Technology also provides a foundation for data analysis related to student performance and instruction/support requirements.
Bill Gates remarked in a recent interview how Microsoft's early success was driven by strategic partnerships. TEL's ability to scale high-quality, affordable learning is also facilitated by key partnerships. These include strategic technology partners, such as MonotrEDU (exam proctoring) and Peerceptiv (peer-review and engagement), who provide cost-effective, specialized technology to support our mission. Sustainable affordability is also supported by our higher education partners, who believe in our model and support our mission with their infrastructure and regional accreditation, and our school partners who provide students with needed local guidance in their learning journey.
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Better Not Ask Why (A Parable)
Episode 1: 5 BIG Problems to Solve in Education
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text:
Q: Symfony Value of a SELECT I have a Select which get a list of string, but i need to insert in database their ID, not their name.
I work under symfony 4.2
In my Form, i use this (which works):

    $builder
        ->add('cours', EntityType::class, array(
          'class' => Cours::class,
          'label' => false,
          'placeholder' => 'Cours',
          'required' => true,
          'choice_label' => 'nomUe',
          'choices' => $formation->getCours(),
          'attr' => array('class' => 'custom-select')
        ))

I looked the documentation and i want to insert something like that

          'choice_value' =>  $formation->getId(),

But that gives me an error like :

And idea? Thank you!

A: in fact you have probably redefined the __toString method in your Class entity .... that's why you have the name in visual in the form ... but in the database it will record the ID of your entity.
so you have to remove the line choices in your form ... so in the end your form should look like this:
    $builder
        ->add('cours', EntityType::class, array(
          'class' => Cours::class,
          'label' => false,
          'placeholder' => 'Cours',
          'required' => true,
          'choice_label' => 'nomUe',
          'attr' => array('class' => 'custom-select')
        ))

However ... I imagine that your __toString method which is in the Class entity looks like this :
public function __toString()
    {
      return $this -> getName() ;
    }

so, if you prefer to have the Id rather than the name of the entity in the form, you'll have to display it:
public function __toString()
    {
      return  (String) $this -> getId() ;
    }

redpajama_set_name: RedPajamaStackExchange


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Travis Hopson
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Review: 'The Parts You Lose', Aaron Paul Plays An Escaped Fugitive In A Dull Thriller That Goes Nowhere
By Travis Hopson10/04/2019View Comments
Everything about director Christopher Cantwell's The Parts You Lose can be summed up in a single sentence: A young deaf boy befriends an injured fugitive and learns life lessons while nursing him back to health. That it's so easily boiled down isn't a problem, all movies should be as simply defined, but it's incumbent upon the filmmaker to do something with it. That's the problem facing Cantwell's slowest of slow burn dramas, is that it lacks necessary tension and stakes when the circumstances tell us there should definitely be some.
The talented group of actors give it their best shot to fully realize the premise's possibilities, chief among them deaf actor Danny Murphy. He plays Wesley, whose life is difficult enough beyond his deafness. There's a severe lack of hope in the downtrodden North Dakota town in which he lives with his supportive mother (Mary Elizabeth Winstead) and younger sister. His abusive, uncaring father (Scoot McNairy) is often away for long spells, and when he returns casts unfair expectations on Wesley, such as stopping all that sign language stuff he does with his mother. Makes him weak, or so he thinks. Wesley gets bullied at school, in particular by one kid who gets kicks from putting boogers in his hair.
Everything changes for Wesley when he discovers a half-dead man (Aaron Paul) lying in the snow. On a whim, he drags the unconscious, unnamed individual to a barn and begins nursing him back to health. Over time they become friends, or something close to it, with Wesley being taught how to defend himself from his abusers (with a backpack full of piggy bank coins) by his gruff, thuggish new father-figure. Meanwhile, we learn from TV reports and occasional visits from the law that the man is a wanted fugitive, and a dangerous one at that.
There's a good story here that could've made for a light-hearted coming of age drama or a tension-packed thriller. Unfortunately, Cantwell seems disinterested in choosing either, with little that happens in the film carrying much dramatic weight. While a competitive game of checkers momentarily teases a much darker side to Paul's character, little else conjures up the high-pressure scenario that having a desperate criminal within arm's reach of a defenseless child and his unsuspecting family should. Even the manhunt is dull, told in such a distant way that we hardly believe there's anything to be concerned over.
Cantwell's unhurried pace may be a bore, but it does offer glimmers of opportunities that sadly go to waste. His use of sound, and the lack thereof, to capture the silence and loneliness of Wesley's world is effective. There's a more engaging story to be found in Wesley trying to navigate a life that is always going to be an uphill climb, so much so that a wayward outlaw seems like a possible way out. But the script by Darren Lemke (who wrote Goosebumps and the upcoming Gemini Man) is thin on details, and under-utilizes its performers. The Parts You Lose is watchable mainly because of the cast's efforts, but has little else to offer.
Rating: 2.5 out of 5
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Covid-19 Update #2
by Francis Miller | Apr 6, 2020 | Blog Entry | 0 comments
Greetings supporters of the Dougbe River School,
As you heard in our last update, we are monitoring very closely how the COVID-19 situation is affecting Liberia, and particularly the Dougbe River School. We wanted to follow up by sharing some news about Executive Director Isaac Monah, who was in Liberia as the virus began making its way around the globe, and becoming a factor in the United States.
Isaac had plans to travel back to the US from Monrovia on Friday, March 20th. His original itinerary would have taken him through Brussels. When Europe began shutting down, he was forced to take an alternate route via Accra, Ghana. After he left Monrovia and arrived at the Kotoka International Airport in Accra, he learned that his connecting airline had suspended operations, thereby canceling his flight to the US. He had to stay in the airport overnight, along with many other travelers from various parts of the world, who were unsure about their flying fates. The Ghanaian government eventually decided to shutdown the airport and Isaac was ushered into government buses and taken to a local hotel for quarantining.
All of the travelers were tested for novel coronavirus. Fortunately, Isaac tested negative. However, he will have to remain in the hotel for 14 days. He will be tested again before he is allowed to leave. (By the time most of you read this, he should be awaiting the results of that follow-up test.) Once cleared, he will be able to stay in a hotel of his choosing, and work with the US Embassy on completing his journey home. He has also been in touch with Sen. Sherrod Brown's office.
Isaac has done his best to stay active in his room, and is keeping close tabs on the operations of the school. He sends his regards to everyone and asks that you continue to pray for his safe passage back to the US, for the students and staff of the Dougbe River School, and all who are suffering because of the virus.
Mercy and peace be with you all,
Francis Miller
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Ministry of Education Reopens Schools in Liberia
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Liberia Has Declared a State of Emergency
Campus Safety Plan for Covid-19 Crisis
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Copyright Dougbe River School, 2020.
Dougbe River School is a 501(c)(3) organization. Gifts are deductible to the full extent allowable under IRS regulations.


My latest post is actually a "page", one of those postings that stands independently from the usual time sequence. It's titled, "Distinctions". I think you'll enjoy it, and hopefully it'll spark all sorts of useful thoughts.
That page links to so much great writing. You really are talented at getting your thoughts across. I will be re-reading some of these.


My philosophy when working with clients is body, mind and soul – and none can be mutually exclusive. So, what does it look like when you come to see me?
When we work together I'll look at the whole you, with a holistic treatment plan. I'll examine dietary, lifestyle and mindfulness influences that contribute to your current state of health, factoring each piece into the bigger puzzle.
An in-depth analysis of your current health state.
Initial consultations range from 60-90 minutes, depending on your case. There's no rushing when it comes to getting to the bottom of your health issue.
We'll dive into your past, to understand your present, to change your future – for the better!
Dietary recommendations and recipe ideas.
I don't provide meal plans as, to blunt, I don't believe they work for everyone. Instead, I'm going to empower you with the right tools to change your life for good, not for a diet.
Information handouts relative to your case.
Knowledge is power, and I'm going to empower you with my knowledge.
Let's dive deep into the unknown to tackle your case head on. No beating around the bush here.
I'll work with you to monitor and track the progress of your case with routine blood tests and ultrasounds (where appropriate).
Tailored to your needs, budget, schedule and lifestyle. It's my mission to make helping you, help yourself, so simple and easy. Fuss free!
My theory goes "more than food, state of mind". As part of our treatment approach we will address emotions, feelings and look at where your state of mind is placed.
I'm a firm believer that supplements cannot replace a healthy diet. Working with me, you'll get to the bottom of your health issues, as we work towards solutions not symptom management. I do believe however the supplementation can support a healthy body and balanced diet. There's no doubting that the world isn't what it used to be, and with more toxins and fewer nutrients in our foods, smart supplementation can be a useful tool.
I work with clients on a broad spectrum of health / disease states, however I do take a special interest in a few. My passion lies with helping women overcome hormonal imbalances, mainly associated with conditions like PCOS.
Available face-to-face in Melbourne at The A Life clinic, or via Skype. BOOK NOW!
Annalise practices at The A Life, 699 Nicholson Street, Carlton North Victoria and is available on Tuesday, Thursday and Saturday.
Still have a few questions? Drop me a line via the form below and we can catch about how I can help you and arrange an appointment from there!
Let me know of any questions you might have. If you are interested in scheduling an appointment let me know of the day and time that is most suitable to you.
"Annalise has been nothing short of amazing. Her knowledge and expertise in nutrition gave my all the right information I needed to make more conscious choices throughout my day, allowing my body to finally feel amazing. She helped with my gut problems, something I've always suffered from; that no one else was ever able to give me adequate advice / information on. I am so happy to finally say I have been feeling the best I ever have, and it's all thanks to this amazing human!"


text:
Q: Creating X509 certificate in C using post-quantum public key algorithm? I'm trying to implement a self signed x509 certificate that uses a post-quantum (PQ) public key algorithm as the public key algorithm. I looked at the openssl library in c, and the way it's done using RSA. I'm essentially trying to replicate the same format. From what I've seen in the openssl library, RSA and a couple of other supported algorithms are integrated in the crypto EVP layer (the key is stored as EVP_PKEY). The functions in the openssl library that I'm trying to use are X509_REQ_set_pubkey(X509_REQ *x, EVP_PKEY *pkey), and some other functions that have very similar inputs. Is there a way that I can integrate the PQ algorithm into the EVP layer? If not, is there any way around using the EVP layer that would achieve the same goal?
I have tried looking into the evp source code in the openssl library. It seems it only supports certain algorithms such as RSA, EC... I'm not sure if it's possible to incorporate the PQ algorithm into the EVP layer. I'm following along the example in this link: (https://www.codepool.biz/how-to-use-openssl-to-generate-x-509-certificate-request.html) to generate the certificate. Instead of RSA key, I just plug in the PQ algorithm key. So far when I create my certificate, it's always outputted in the wrong format.
I'm using this command: openssl x509 -in x509Req.pem -text -noout to read the certificate generate. It always shows the error message
unable to load certificate \n 140688586052032:error:0906D06C:PEM routines:
PEM_read_bio:no start line:../crypto/pem/pem_lib.c:691:Expecting: TRUSTED CERTIFICATE

A: The EVP API uses a "ENGINE" for it's implementation of all the symmetric algorithms (ciphers), digests and asymmetric algorithms (public key algorithms). A ENGINE module can be added / replaced.  See openssl engine command.
I think this is the area you want to look into.  I don't think there is a lot of documentation around this area (that I could find) but there are sample engines you can look into.
If you download the openssl source you can look in the engines folder for sample engines you can use to start to implement you custom algorithm(s).
Good luck!


I want to thank Henny Blomme for the time and effort he spent on my book, and for his insightful comments (see here). His objections could be roughly divided into two groups: one concerns my distinction between realism and anti-realism, and the other concerns my interpretation of things in themselves. I will reply to them in turn.
The goal of Chiba's book is to answer the following question: Is Kant's 'transcendental idealism' 'realism' or 'idealism'? (Chiba 2012:2) Chiba concludes that Kant is an anti-realist: objects do not exist independently of our cognition. Chiba's book contains a lot of interesting and precise analysis of parts of Kant's argument in the Critique of Pure Reason. So it should be clear that the following remarks, although they express disagreement or reservation, are in no sense meant to hold back anyone from reading Chiba's book. The contrary is the case: although I do not agree with everything Chiba affirms and defends, I can recommend his in its detail very informative dissertation to anyone who is interested in the attempt to link Kant's theory to the contemporary 'realism' debate.


text:
Q: Simulate different network condition in docker Run test using Selenium + Chrome that run inside a Docker container, (Host is Ubuntu)
Need to simulate different network condition for each container, in and out bandwidth, latency, firewalls. can I use tool like https://github.com/tylertreat/Comcast on each container and it will not effect the networking condition of other containers?
Is there a better way to do that?
I see this https://github.com/docker/docker/issues/37 but not clear how to use it.

A: I'm using this command to affect only the network conditions of one container:
sudo nsenter -t PID -n comcast --latency=2000 --target-port=9200

PID needs to be one of the PIDs of the container
nsenter ... -n enter in the network namespace of the container

A: Found this seem to be just working,
docker run with --privileged=true
in the docker image can use any program that work to shape the network traffic, worked for me comcast, tc and iptables
each docker get his own network profile and one container does not effect other containers.
see also
https://github.com/tylertreat/comcast/issues/48#issuecomment-326088625


Are your customers having a hard time managing and backing up data? Without the appropriate tools, data protection becomes unnecessarily complicated, risky and wasteful. While no serious company can afford to take data protection lightly, no agile company can afford to bury its IT crews in data management.
Conventional backup and recovery tools are inflexible. They cannot be scaled across a large number of multitenant clients and do not offer a centralized console to manage any and all of your data management functions. They are sluggish, error-prone, and just another pain to manage. They're the reason why sometimes companies fail to backup and lose critical data. But your clients can do better.
Now you can offer your clients the protection, productivity, and peace-of-mind they want from a data management tool. Acronis Cloud Backup is the market leader in automated Cloud based data protection, backup and recovery. It's the tested and proven solution that's already on top of your clients' shopping list. And now you can sell it to them at zero Capex.
Don't you worry about a thing! Acronis AnyData Engine is the industry's most robust, complete and diversified backup technology that stores data locally as well as on the Cloud. With automated, single-point data management and bare-metal recovery, the data isn't going anywhere.
Acronis Backup Cloud is the industry-unique solution built from ground up for MSPs, ISPs, CSPs and other service providers. Manage customers from a unified point on up to three rebrandable channels levels, with unlimited tenants on every channel level under one partner subscription.
The easy-to-use, powerful Acronis Backup doesn't only make life easier for your clients, but also brings you easy profits and long-term customers. Expand your portfolio and start earning incremental revenues with no upfront cost. For a monthly usage based price, you and your clients will find it extremely affordable.


Royal Bank of Scotland has risked sparking anger amongst employees after increasing contributions for its final salary pension scheme from zero to 5pc of salaries.
Over 40,000 employees were told on Monday they would need to make contributions if they wanted to retire with their full entitlement at the age of 60. Staff will be able to retire with full pensions whilst paying no contributions if they continue to work until 65.
The change was branded "unaffordable" by Unite national office David Fleming. He said: "With 28,000 workers receiving no pay rise this year, these changes will make access to the pension scheme unaffordable for many. The bank is attempting to push through these changes without any proper negotiations with the union.
The change affects those staff members who joined before 2006 when the final salary scheme was closed.
The decision to penalise rank and file employees is bound to attract criticism given the level of bonuses still enjoyed by senior investment bankers.
The state-owned bank paid £785m in bonuses last year although chief executive Stephen Hester decided to forego his payout.


It's finally time to put that new bounty system to use. Yes, it's been coming for several weeks but now we're finally ready to turn Tanoa into a war zone again. In effort to keep things on Tanoa island fresh and exciting we would like to announce the 24-hour player vs player (PvP) event!
That's right - PVP day is upon us! Starting 13:00 on 2017-09-01 through to 13:00 on 2017-09-02 all of Tanoa will be a PVP zone.
Those of you who have lived in the serenity of the PVE zone can expect your once-friendly island inhabitants to think twice before sharing a mission or backing out of loot in the interest of fairness. Frankly, I'd expect them to murder you on sight. For 24 hours Tanoa will become it's historic alter-ego and become World War 2.
Otherwise, please feel free to shoot on sight. You will stand as owner of the land or kneel like peasants. The choice is yours. You have one week to decide!


The Grey Bruce Woodturners Guild has a long history of comraderie amongst the wood turners of Grey and Bruce Counties in the province of Ontario in Canada. By November of 1992, with the help of Steve Krauss at the regional Welbeck's Saw Mill, Ken Goldspink had gathered a list of names in Grey and Bruce counties who were interested in forming a wood turning guild. Eventually, the inaugural group met at the home of Clive Taylor near Kemble. Some of the founding members include Clive & Margy Taylor, Ken Goldspink, Bob Hastings, Steve Smith, Dan Braniff, the late Jim O'Dell, Michael Armstrong, and the late Dave Saker, and some unknown others. The first executive of the guild included President Clive Taylor as President, Margy Taylor as Secretary, and Ken Goldspink as Treasurer.
In the early days, monthly guild meetings were rotated among member homes, automatically providing an opportunity to see the various member workshops. Later, the guild found a home in the Saugeen District Secondary School in Port Elgin. The arrangement included use of the school's lathes, in return for routine maintenance on the equipment. In the spring of 2005, the guild moved to its present location, the old firehall in Kincardine. As part of its grand opening in the summer of 2005, guest turner Lyle Jamieson provided a week-long workshop on bowl and hollow turning, with participants from other Ontario woodturning guilds. Oneway kindly loaned a 2436 lathe for this workshop.