text:
2019 Tours
Tour with Bill Landry
Tour with Sam Venable
Overnight to Tennessee Virginia Border
Festive Day Trip to Biltmore and The Grove Park Inn
TOURS OF THE SMOKY MOUNTAINS
Public & Private Tours of Cades Cove and More
Cades Cove Tour (Open to the public)
LOCATION: Departs at 1:30 p.m. from the Great Smoky Mountains Heritage Center in Townsend, Tennessee
Sat. Jun 1, 8, 15, 22, 29
Sat. July 6, 13, 20, 27**
Sat. Aug 17, 24*, 31
Sat. Sept 7, 14, 21, 28
Sat. Oct 5, 12, 19
Sat. Nov 2, 9, 16, 23
*SOLD OUT
**CANCELLED
TOUR LENGTH: Approximately 4 hours (length can fluctuate depending on vehicle traffic in Cades Cove)
Max # of guests = 18
CLICK TO LEARN MORE ABOUT THIS GREAT TOUR
TOUR DATES: Tours run on Saturdays from June through November. See below for specifics.
Saturday June 1, June 8, June 15, June 22, June 29
Saturday July 6, July 13, July 20, July 27
Saturday, Aug. 3, Aug. 17, Aug. 24, Aug. 31
Saturday Sept. 7, Sept. 14, Sept. 21, Sept. 28
Saturday Oct. 5, Oct. 12, Oct. 19
Saturday Nov. 2, Nov. 9, Nov. 16, Nov. 23
Experience the beauty and history of Cades Cove
Nestled in the heart of the Great Smoky Mountains, you'll find Cades Cove. It's an area lush with history, wildlife and some of the most beautiful views you'll find in the park. An expert guide will take you on a memorable and education experience perfect for a Saturday afternoon.
Whether you're a regular visitor to Cades Cove or this is your first time, there's always something new to learn or see. Discover the history of the Cove and share in the personal stories of its people. You'll also learn about the ecology, flora and wildlife of the region.
A tour through Cades Cove will offer you a rare chance to turn off your phone and reconnect with nature in one of the Smokies' most popular spots. Take a journey into the past as you explore the lives of those who once lived in the Cove and witness its natural wonders in the flora and fauna that grow there.
Make sure you bring your camera!
The Cove features several historic buildings, including three churches, log homes, a working grist mill, and other restored structures. You'll be paying a visit to several buildings on the tour, including the Cable Mill, which is one of the Cove's most popular spots.
The wildflowers of Cades Cove draws visitors each year. Whether it's mountain laurel, rhododendron, flame azaleas or another type of flora, the Cove is unmatched in its beauty.
Different seasons offer different opportunities for photos. In the winter, guests can expect unexpected sights, as the trees have shed their leaves. In the spring, you can expect beautiful, lush vegetation.
ACTIVITY RATING: easy, moderate, challenging, demanding
ACTIVITY DESCRIPTION: The tour will not involve hiking or very much walking, but visitors should be advised the tour does make frequent stops at several sites. Attendees will be entering and exiting the tour vehicles multiple times, and there are also some areas of uneven surfaces. Attendees will also have to climb a few steps to enter buildings in the Cove.
WHAT TO WEAR: In addition to wearing (or bringing) sunscreen, it's best to dress comfortably. Due to the nature of the tour, we recommend you wear comfortable shoes so you'll be able to enjoy the stops along the way comfortably. Also, because the tour will continue rain or shine, we suggest you bring a rain jacket, just in case.
WHAT TO BRING: Feel free to bring a camera along for the many photo opportunities you'll see along the tour. You're also welcome to bring along non-alcoholic drinks — we recommend plenty of water — or snacks, but be aware restroom facilities are limited in the Cove. A restroom break will be available halfway through the tour at Cable Mill. You can also bring binoculars, a notebook or related items to make the most of your experience. We do suggest having a backpack handy for these items, though, to make exiting the vehicles as simple as possible.
RESERVE YOUR SPOT: Cades Cove tours are limited to 14-18 guests. Click here or call us at 865-448-8838 to make your reservation.
WEATHER POLICY: We reserve the right to cancel or reroute tours up until the last minute due to weather, safety, mechanical failure or other uncontrollable circumstances. In this case we will try to reschedule, give rainchecks, agree on another more accessible destination, or, if necessary, give a full refund. We also wait until the last minute to make these calls. We do not speculate on forecasts.
CANCELLATION POLICY: In order to cancel or reschedule a reservation without fees and penalties, call us no less than one week in advance. Refunds will not be given for any change made with less than a week's notice.
GENERAL INFORMATION: Cades Cove Heritage Tours is a private non-profit that seeks to provide a superior visitor experience while protecting the natural and cultural resource of Cades Cove. We offer visitors the choice to take a relaxing guided tour and learn about the natural resources and human history in the Cove, all without having to worry about "who's going to drive?"
Cades Cove Tour Private Tours
We would be happy to schedule a private tour for a minimum payment of $200 which entitles you to a tour of Cades Cove for up to 10 guests.
Arrangements for private tours should be made as far in advance as possible.
Same day tours are unlikely to be arranged.
$200/group
Up to 10 guests on a day and time mutually agreeable to your group and Cades Cove Heritage Tours.
Additional guests over 10 are charged $20 up to the maximum capacity of our vehicle.
Cades Cove with Bill Landry
Departs from the Great Smoky Mountains Heritage Center in Townsend – See Departure Times Below
Approx. 4 hours depending on traffic in Cades Cove
This is your opportunity to join Bill Landry of "The Heartland Series" fame for a wonderful guided trip around Cades Cove, while Bill shares stories, tales and adventures of the cove.
Saturday, July 20: 9:30 am
Saturday, Aug. 10: 9:30 am
Saturday, Aug. 10: 2:30 pm
Saturday, Sept. 7, 9:30 am
Saturday, Nov. 9: 9:30 am
All Tours Departs at 9:30 am (except the added 2nd Aug. 10th tour, which departs at 2:30 pm) from the Great Smoky Mountains Heritage Center in Townsend
Cades Cove Tour with Bill Landry
COST: $65 per person (reservations required)
LOCATION: Departs at 9:30 a.m. from the Great Smoky Mountains Heritage Center in Townsend, Tennessee
TOUR DATES: Tours with Bill Landry have limited availability. See below for specifics.
Saturday, Nov. 9
Tour Cades Cove with one of East Tennessee's most beloved storytellers!
Join longtime host and narrator of WBIR's the Emmy award-winning "The Heartland Series" for a guided trip around Cades Cove. Even if you've seen the Cove before, the celebrated author, producer and actor will make this an experience not to be missed.
Bill will offer his unique brand of humor and Appalachian storytelling as you travel together through the Cove. You'll have the opportunity to learn the history of the Cove and its people while admiring the beauty of the ecology and wildlife you discover along the way.
Bill has plenty of tales to share about the mountain folk who called the Cove home. He'll also share interesting stories about the animals and other rarities that keep visitors coming to Cades Cove each year.
Cades Cove is teeming with wildlife. It's not unusual to see white-tailed deer, black bears, coyotes, foxes, turkey or other animals while on tour. While it's best not to approach or disturb the wildlife you see, they make for a great photo opportunity.
ACTIVITY DESCRIPTION: The tour will not involve hiking or very much walking, but visitors should be advised the tour does make frequent stops at several sites. Attendees will be entering and exiting the tour vehicles multiple times, and there are also some ares of uneven surfaces. Attendees will also have to climb a few steps to enter buildings in the cove.
WHAT TO BRING: Feel free to bring a camera along for the many photo opportunities you'll see along the tour. You're also welcome to bring along non-alcoholic drinks — we recommend plenty of water — or snacks, but be aware restroom facilities are limited in the Cove. A restroom break will be available halfway through the tour at Cable Mill. You can also bring along binoculars, a notebook or related items to make the most of your experience. We do suggest having a backpack handy for these items, though, to make exiting the vehicles as simple as possible.
RESERVE YOUR SPOT: Tours with Bill Landry are limited to 17 guests per tour. Click here or call us at 865-448-8838 to make your reservation.
Cades Cove "Behind the Scenes Tour" with retired National Park Service ranger Mike Meldrum
Gregory's Cave and the thriving business of Joe Gregory
The story of the Primitive Baptist Church
Managing wildlife in Cades Cove and the Deer Exclosure
The CCC Camp
The WW II Memorial Tree
Thursday October 3
(Includes Box Lunch)
18 Guests Maximum
Departs GSMHC at 9:30AM
Returns Approximately 4:30 – 5:00PM
COST: $65 per person with box lunch included — choice of ham, turkey or salad (reservations required)
TOUR DATES: Tours with Mike Meldrum have limited availability. See below for specifics.
No one knows Cades Cove like a retired national park ranger!
With nearly 20 years of service in Great Smoky Mountains National Park, retired ranger Mike Meldrum is what you might call an expert on the Smokies. A tour with Mike will take visitors to exclusive locations in the Cove, including Gregory's Cave, the CCC Camp, the World War II Memorial Tree, Cable's Lodge and more.
Visitors will also have the chance to hear about Joe Gregory's thriving business.
Back in the day, Joe used to charge visitors 50 cents to explore the cave. Earlier, it was used by Native Americans and Americans alike for various purposes. Aside from reported Civilian Conservation Corps moonshine parties and dances, the cave also hosted a government fallout shelter during the Cold War.
Guests will also be able to learn about the hidden history of Primitive Baptist Church and more.
Many more opportunities await visitors. Just let your imagination run wild.
RESERVE YOUR SPOT: Tours with Mike Meldrum are limited to 17 guests per tour. Click here or call us at 865-448-8838 to make your reservation.
Great Smoky Mountains National Park from Townsend to Sugarlands to Newfound Gap w/ Sam Venable
Join Sam Venable, noted author, columnist, humorist and naturalist, on a trip through the Great Smoky Mountains National Park from Townsend to Elkmont, Sugarlands, and Newfound Gap.
Approximately 4 – 4/12 hour tour
Great Smoky Mountains National Park Tour — from Townsend to Sugarlands to Newfound Gap — with Sam Venable
TOUR LENGTH: Approximately 4 hours (length can fluctuate depending on vehicle traffic in Great Smoky Mountains National Park)
TOUR DATES: Tours with Sam Venable have limited availability. See below for specifics.
Saturday, Sept. 28
Celebrated author, humorist offers unique look at Smokies
For more than four decades, Sam Venable has been amusing readers around East Tennessee as an award-winning writer at the Knoxville News-Sentinel. While Sam might be a humor columnist now, he got his start as an outdoors writer for the paper.
He's also the author of several books and a local, state and national award winner for his writing. All this goes to show the folks on tour with Sam are in for a memorable adventure and some interesting tales.
The naturalist will lead visitors on a trip through Great Smoky Mountains National Park with stops planned in Sugarlands and Newfound Gap.
Great Smoky Mountains National Park is teeming with wildlife. It's not unusual to see white-tailed deer, black bears, coyotes, foxes, turkey or other animals while on tour. While it's best not to approach or disturb the wildlife you see, they make for a great photo opportunity.
Sugarlands and Newfound Gap will offer plenty of chances for a photo of flora and fauna. Many more opportunities await visitors. Just let your imagination run wild.
RESERVE YOUR SPOT: Tours with Sam Venable are limited to 17 guests per tour. Click here or call us at 865-448-8838 to make your reservation.
Bear Viewing w/ Retired NPS Wildlife Resource Officer Kim Delozier
Author, naturalist and former Park Ranger, Kim DeLozier will point out and discuss wildlife in the Smokies. Kim is the author of "Bears in the Back Seat I and II", humorous and insightful books on Smoky Mountain black bears.
Departs GSMHC at 4:30PM
Create a one-of-a-kind experience for your party!
Have a wedding, group or special occasion coming up? Cades Cove Heritage Tours can make the day even more memorable by offering shuttle service for you and your party.
Whether your group needs a ride from your wedding to the reception or even to and from the rehearsal dinner, we have you covered. Whatever your event, leave the driving to us as you enjoy a relaxing, safe ride to your destination.
Weddings * Rehearsal Dinners * Special Events * Meetings
Create a one-of-a-kind experience for your party
This service is not limited to weddings, though. We offer shuttle service throughout the Townsend, Walland, Gatlinburg, Pigeon Forge and surrounding area for all of your special events, including meetings and other gatherings. The trip is completely customizable to meet your needs. You set the number of guests, date, hours and drop-off/pickup locations.
Please note: For your safety and security, our experienced drivers will remain on site in case emergency travel is required.
RESERVE YOUR TOUR: Call us at 865-448-8838 for a quote.
WEATHER POLICY: We reserve the right to cancel or reroute tours up until the last minute due to weather, safety, mechanical failure or other uncontrollable circumstances. In this case we will try to reschedule, give rain checks, agree on another more accessible destination, or, if necessary, give a full refund. We also wait until the last minute to make these calls. We do not speculate on forecasts.
Explore the Tennessee & Virginia Border for a 2 Day / 1 Night Tour
See a Broadway Show, Stay at a Historic Inn, Explore the Highlands Festival and Touch Million Year Old Fossils.
View Tour Flyer
Thursday, July 25 through Friday, July 26
Reservations and payment are required on or before June 20, 2019. No refunds after June 20, 2019.
Limited to 24 guests.
$399 rate per person includes all transportation, taxes, gratuities and service fees. Based on double occupancy. (Single occupancy is available at $590 per person.)
Exploring the TN / VA Border
See a play, stay at an Historic Inn, visit the Birthplace of Country Music, explore fossils from a millennia ago and more!
COST: $399 rate per person includes all transportation, taxes, gratuities and service fees. Based on double occupancy. (Single occupancy is available at $590 per person.)
LOCATION: Departs at 7:30 a.m. from the Great Smoky Mountains Heritage Center in Townsend, Tennessee. Check-in begins at 7 a.m.
TOUR LENGTH: Two days, one night
TOUR DATES: Thursday July 25 and Friday July 26
See a Broadway show, visit a festival and become a paleontologist for a day!
Cades Cove Heritage Tours offers a two-day, one-night tour that will offer something for everyone. After departing from the Heritage Center in Townsend, you'll travel to the Birthplace of Country Music Museum in Bristol, Virginia, where you'll enjoy a box lunch and have an opportunity to explore where it all began.
From there, you'll travel to the historic Barter Theatre in Abingdon, where you'll enjoy playwright Paul Elliott's "Exit Laughing." You'll round the night out at the Martha Washington Inn, where you'll enjoy dinner and spend the night.
The day includes a visit to the Virginia Highlands Festival where you'll enjoy the morning and lunch. You'll round out the day with by traveling back millions of years once you arrive at the Gray Fossil Museum, where you'll have the opportunity to learn about an active Pliocene-era fossil dig site, learn what fossils have been recovered (so far) and even hold one in your hand. The group will head back to the Heritage Center upon leaving the museum.
RESERVE YOUR SPOT: Overnight tours are limited to 24 guests.. Click here or call us at 865-448-8838 to make your reservation. Deadline is June 20. Rate is based on double occupancy. Single occupancy is available at $599 per person
CANCELLATION POLICY: In order to cancel or reschedule a reservation without fees and penalties, call us prior to June 20, 2019. Refunds will not be given for any change made after June 20, 2019
Festive Holiday Trip to Grove Park Inn and Biltmore
27th Annual Gingerbread House Exhibit at Grove Park Inn
Holiday Champagne Brunch at Grove Park Inn
Dowton Abbey Exhibit
Candlelight Tour at Biltmore
Reservations and payment are required on or before October 22, 2019.
Reservations must be received by Tuesday, October 22, 2019
$249 rate per person includes all transportation, taxes, gratuities and service fees.
Join us for a festive Holiday Trip to Grove Park Inn and Biltmore
Stroll thru the Juried 27th Annual Gingerbread House Exhibit & relax during the Holiday Champagne Brunch at Grove Park Inn. Immerse yourself in the splendor of the exclusive Downton Abbey Exhibit at Biltmore and create lasting memories of the decorated Biltmore House while enjoying the beauty of thousands of sparkling lights and the sounds of the Holidays during our Candlelight Evening Tour including your own personal audio guide at Biltmore.
COST: $249 rate per person includes all transportation, taxes, gratuities and service fees.
LOCATION: Departs at 7:00 a.m. from the Great Smoky Mountains Heritage Center in Townsend, Tennessee. Check-in begins at 6:30 a.m.
TOUR LENGTH: One Day
TOUR DATES: Saturday, November 23, 2019
Click here or call us at 865-448-8838 to make your reservation. Deadline is October 22.
CANCELLATION POLICY: In order to cancel or reschedule a reservation without fees and penalties, call us prior to October 22, 2019. Refunds will not be given for any change made after October 22, 2019
Cades Cove Heritage Tours
info@cadescoveheritagetours.org
Townsend, TN 37882-0268
Tour Sponsors
Jenny and Randy Boyd
Shuttle Services from Townsend, Walland, Maryville, Alcoa, & Blount Country
Ask for Don Alexander
Copyright © 2020. Cades Cove Heritage Tours. All Rights Reserved.Website design and hosting by MSW Interactive Designs LLC. We put the web to work for you!


Home › business › Release date for E.P.
I've decided that September 7th will be the release date for my E.P. or more like when I upload it to Bandcamp.
Reason for that date is, it's my birthday and it will be my 45th, so I thought that would make this accomplishment for myself a bit more memorable.
Last week I finished everything on the E.P. except finalising the cover art, which was finding a photo of my girlfriend Estrel to put on the cover and writing her name on the cover as well, as she sang on three of the songs and co-wrote one of them.
I thought it was a good idea to have Estrel listen to the final mix of my/our work, as there were two songs she hadn't heard before.
On first listen to the song "I", she felt something was missing, "Her vocals", which was great to hear as I wanted her to sing on this song anyway, but time and opportunity had been against us and I wasn't confident she would like the song as it was different compared to other songs we have done in the past.
With only Three weeks remaining before I completely finish with this project, we now have to get stuck into sorting out her vocal parts for the song. We've made a good start to it over the past couple of days and I feel confident she will take the vocal section of this song to another level with what she has to offer.
Though we are both not great singers, and singing being my weakest area, Estrels vocals will add that something extra on top of what I have to offer, which is going to be a bonus.
I look forward to wrapping up this project and having a rest before moving on to my next adventure. I will also continue to improve upon my website "readyforjammin.com". I have more basic guitar lessons for beginners I want to upload there, strategies and ideas to help people with their songwriting in general.
I also want to add more ideas to recording as cheaply as possible in this budget tight world we live in, but at the same time have the highest possible quality sound and music that can be made, and most importantly, interaction from other songwriters for their tips and ideas for new and established songwriters who may be struggling with their art for what ever the reason.


text:
School deals with few altercations, serious crimes
Jack Stornello, Staff Reporter
What can be done to solve the issue of violence? It's been a significant problem in Chicago for years, showing no signs of slowing down. There's a high likelihood you've already heard reports of over a thousand acts of gun violence in the city this year. It's widely known that violence is a major dilemma in the city, but what about around here, a little closer to home? Furthermore, how do we go about rectifying the situation?
To be able to devise solutions effectively, you must first understand the causes. You must fully grasp the reasons why these things happen in order to fix them with any degree of success. An approach from both a psychological standpoint as well as a law enforcement one must be taken.
"I think there's various things," Dr. Marla Carson said. "I think [with] students a lot of times there's a relationship issue that maybe people are fighting over. Somebody, obviously on the outside there are probably gang issues that spark the violence. I think people may be having difficulty with managing their anger for whatever reason."
"I think some of the stuff here at Elk Grove, sometimes you have personality conflicts, school related stuff, something's said on social media, friendships come and go and dissolve quickly," Officer Shaun Berens said. "I think a lot of the physical altercations we have here at school are a result of that."
Once the underlying source of the problem is acknowledged, steps can be taken to address it. According to Dr. Carson, school psychologist, several solutions present themselves in the form of "teaching coping strategies, teaching anger management skills, [and] getting people together to work out differences with a mediator."
Continually, social media seems to play a key role in both the causes and solutions to this issue.
"I think mediation and people having conversations with each other about stuff goes a long way," Berens said. "Also, telling an adult if it's something you can't handle on your own. Having said that, also, don't let social media be such a major part of your life. If you feel someone's saying something about you, it's a good idea to talk about those things and not resort to anything physical."
As to how prevalent an issue this really is within the school, things appear to be looking up. Both Berens and Carson concur in the belief that violence in our local area is on the decline.
"I don't really know much about Elk Grove in general, because I don't live in the community," Carson said. "But I think our fights have been down significantly over the past few years, so I don't hear too much about them."
"The high school itself, this is my second year, we've had a handful of fights," Berens said. "But the amount of serious crimes are few and far between. In case there is an altercation, they both can be charged under local ordinance. I wouldn't say there are violent tendencies within the building."
Ultimately, while violence is a major issue closer to the city, our local area appears to be comparatively safe. Knowing that not only is the problem not exceedingly prevalent, but that there are also many ways to remedy said problem when it does arise, should provide many with a certain degree of comfort. With information such as this, one can undoubtedly sleep much better at night.
How social media and pop culture influence student career choices
In-Depth: New block schedule format has pros, cons
Students feel impact of bus driver shortage
Elk Grove Guardian, Volume LIV, Issue II
In-Depth: How remote learning has affected teacher-student communication
Opinion: Students, now more than ever, need better access to mental health resources
How EG teachers are assessing students during hybrid learning
EGHS Guardian Volume LIV, Issue I

redpajama_set_name: RedPajamaCommonCrawl


Stand out from the crowd with a touch of distinction. Ray Allen Custom Agitation Leads provide all the advantages of our Classic Agitation Leads plus, they are braided for a more stylish appearance. The same thing goes with our Tac-Black models.
Ray Allen Custom Agitation Leads provide all the advantages of our Classic Agitation Leads plus, they are braided for a more stylish appearance. The same thing goes with our Tac-Black models. The Tac-Black Leather Braided Handle Lead is 7/8" wide, 60" long and comes with our premium 1" stainless steel bolt snap.


text:
Poroporoaki: Carmen - Hon Tariana Turia
Thursday, 15 December 2011, 2:05 pm
Press Release: The Maori Party
The Hon Tariana Turia
Maori Party Co-Leader | MP for Te Tai Hauauru
Co-leader of the Maori Party has acknowledged with sadness, the passing of one of New Zealand's widely celebrated drag queen, Carmen.
"She literally created history when she opened Carmen's International Coffee Lounge in the late sixties. It quickly became an established part of the Wellington landscape due to the unique character of its founder".
"Carmen was larger in life in so many respects" said Mrs Turia. "When she walked in the room you always had a very strong sense of her presence".
"People from all walks of life respected her and appreciated the warmth and the compassion that she would share with them".
"We knew her as Trevor Rupe from Taumarunui; we knew her as a loved member of the gay community, we knew her as an HIV/AIDS activist; and of course the world knew her as a flamboyant, incredible performer on stage".
"Carmen strode through life with an attitude which told everyone that no-one could make her feel inferior or judge her as less than their equal".
"Her decision to contest the Wellington mayoralty; her entrepreneurial flair and her determination to speak her mind remain a lasting legacy of her role in our development as a nation".
"I will never forget my aunt telling me, when Carmen came to Whanganui, that our focus should not be on promoting her as an iconic stripper and transgender celebrity – even though that was very much part of who she was.
My aunt encouraged us instead to focus on celebrating Carmen for all that she was – for all of her unique attributes and the wonderful person that she was".
"And so today, I am sure I join with many who shed tears for Carmen, the queen, the friend, the whanau member, the legend".
Find more from The Maori Party on InfoPages.


Eight new tracks, conceived and recorded throughout 2018 by CRONACA NERA and mastered by Francesco Perizzolo aka "Dr. Pery" (the mastermind behind Satanismo Calibro 9), including a the Necrofilia cover: "Essere per la Morte" (Necrofilia was a collaborative project by Atrax Morgue and Teatro Satanico). Cronaca Nera in this album, as in previous ones, resumes the sound of Italian Industrial Power Electronics of the 90s with a powerful use of the Voice by Andrea Chiaravalli (Iugula-Thor) and Adriano Voncenti (MMM) screaming and telling stories, where violence is the choosen tool for expression. This will be the last album by the trio, composed by Adriano Vincenti (Macelleria Mobile di Mezzanotte), Giovanni Mori (Le Cose Bianche) and Andrea Chiaravalli (Iugula-Thor). Album co-released with LUCE SIA.


Available on a USB flash drive, Switched-On Schoolhouse is a Christian homeschool curriculum provided by Alpha Omega Publications. SOS Fundamentals of Computer Systems uses vivid graphics, videos clips, audio files, animation, and learning games to liven up lessons and clarify concepts. SOS also offers automatic grading and recordkeeping, as well as a customizable school calendar.


text:
Q: Serializing an Object that contains 3 lists of different objects Protobuf-net I've been reading up on the capabilities of this package and I was just wondering if it is even possible to serialize an object which contains 3 lists of different objects, or if I would be wasting my time trying to switch over.

A: We ended up switching to MSGPack instead of PhotoBuf, it was much easier to use with better documentation and had no problem serializing the data in it's current format. After wrestling with PhotoBuf for a few hours, I got MSGPack working in 25 mins in C# and got it receiving and deserializing the data in JS after about an hour. Great choice to switch!

redpajama_set_name: RedPajamaStackExchange


text:
from __future__ import absolute_import, division, print_function, unicode_literals

from echomesh.Cechomesh import cechomesh

import itertools

from echomesh.pattern.Pattern import Pattern
from echomesh.util.string.Plural import plural

class List(Pattern):
    HELP = 'Display a spread of colors between two or more color endpoints.'
    CONSTANT = True
    PATTERN_COUNT = 0

    SETTINGS = {
      'colors': {
        'default': [],
        'help': 'A list of colors to be displayed.',
        },
      'columns': {
        'default': 0,
        'help': 'The number of columns in the result',
        },
      }

    PATTERN_COUNT = 0

    def _evaluate(self):
        colors = self.get_raw('colors')
        has_lists, has_scalars = False, False
        for c in colors:
            is_list = isinstance(c, list)
            has_lists = has_lists or is_list
            has_scalars = has_scalars or not is_list

        if has_scalars:
            if has_lists:
                raise Exception('Can\'t mix lists and scalars in pattern.List')
            colors = [colors]
        max_column = max(len(c) for c in colors)
        columns = self.get('columns') or max_column
        if not max_column:
            # Empty list.
            return cechomesh.ColorList(columns=columns)

        ce = (cechomesh.color_list_with_errors(c) for c in colors)
        color_lists, errors = zip(*ce)
        errors = list(itertools.chain(*errors))
        if errors:
            raise Exception('\nCan\'t understand %s: %s.' % (
                plural(len(errors), 'color'), ', '.join(errors)))
        result = cechomesh.ColorList(columns=columns)
        for i, cl in enumerate(color_lists):
            cl.size = columns
            result.extend(cl)
        return result

redpajama_set_name: RedPajamaGithub


text:
Q: AppleScript : calcule how many day from two date I need to calculate the date between two dates, and tell me how many day are in between, if more than 30 days then I will target something.
in this script D is the date (in the past) that I want to calculate from today
  set X to MYdatefromSafari -- "August 26th, 2016"

    set D to ConvertDate(X)
    log D

    on ConvertDate(X) -- sub routine to convert string "english_month dayth/st, year" to real date
        set MS to {"January", "February", "March", "April", "May", "June", "July", "August", "September", "October", "November", "December"}
        set LW to every word of X
        if (count of LW) is not 3 then return "" -- invalid format
        set MI to 0 -- check month : should be in the list
        repeat with I from 1 to 12
            if item I of MS is item 1 of LW then set MI to I
        end repeat
        if MI is 0 then return "" -- the fisrt word is not in the list of months
        try -- check day : it should be NNth of NNst
            set DI to (text 1 thru -3 of item 2 of LW) as integer
        end try
        if not ((DI > 0) and (DI < 31)) then return "" -- invalid day
        try -- check year
            set YI to (item 3 of LW) as integer
        end try
        if not ((YI > 0) and (YI < 9999)) then return "" -- invalid year
        return date ((DI & "/" & MI & "/" & YI) as string)
    end ConvertDate

In the best scenario, that would calculate the number of date in between if less than a year, and month or year if more

EDIT :
set X to "August 26th, 2016"
set MyDate to ConvertDate(X)

set D to ConvertDate(X)
log D

set SecondDate to (current date) -- = system date
set ListDiff to DateDiff(D, CD) -- returns {diff days, diff months, diff years}
log "Days = " & item 1 of ListDiff
log "Months = " & item 2 of ListDiff
log "Years = " & item 3 of ListDiff

on DateDiff(D1, D2) -- return list with difference in days, in months, in years
    -- depending if differences is less than month, or less than year or higher than a year
    if D1 > D2 then -- set DStart as oldest date
        copy {D1, D2} to {Dend, DStart}
    else
        copy {D1, D2} to {DStart, Dend}
    end if
    return {(Dend - DStart) div days, (Dend - DStart) div (30 * days), (Dend - DStart) div (365 * days)}
end DateDiff

on ConvertDate(X) -- sub routine to convert string "english_month dayth/st, year" to real date
    set MS to {"January", "February", "March", "April", "May", "June", "July", "August", "September", "October", "November", "December"}
    set LW to every word of X
    if (count of LW) is not 3 then return "" -- invalid format
    set MI to 0 -- check month : should be in the list
    repeat with I from 1 to 12
        if item I of MS is item 1 of LW then set MI to I
    end repeat
    if MI is 0 then return "" -- the fisrt word is not in the list of months
    try -- check day : it should be NNth of NNst
        set DI to (text 1 thru -3 of item 2 of LW) as integer
    end try
    if not ((DI > 0) and (DI < 31)) then return "" -- invalid day
    try -- check year
        set YI to (item 3 of LW) as integer
    end try
    if not ((YI > 0) and (YI < 9999)) then return "" -- invalid year
    return date ((DI & "/" & MI & "/" & YI) as string)
end ConvertDate

A: Sub-routine "DateDiff" bellow gives back a list of 3 difference values : in days, months and years.
Set X to MyDatefrom Safari
Set MyDate to ConvertDate(X)

set SecondDate to (current date) -- = system date
set ListDiff to DateDiff(D, CD) -- returns {diff days, diff months, diff years}
log "Days = " & item 1 of ListDiff
log "Months = " & item 2 of ListDiff
log "Years = " & item 3 of ListDiff

on DateDiff(D1, D2) -- return list with difference in days, in months, in years
-- depending if differences is less than month, or less than year or higher than a year
if D1 > D2 then -- set DStart as oldest date
    copy {D1, D2} to {Dend, DStart}
else
    copy {D1, D2} to {DStart, Dend}
end if
return {(Dend - DStart) div days, (Dend - DStart) div (30 * days), (Dend - DStart) div (365 * days)}
end DateDiff

on ConvertDate(X) -- copy your existing sub-routine
end ConvertDate

For instance if MyDate = Jan 20th 2016 and we are August 26th 2016, it will return {219, 7, 0} because différence is 216 days or 7 months (Jan to August) or 0 year (2016 both dates !).


The Corte San Benedetto Winery is located in Arbizzano di Negrar inside the ancient rural court from which the winery took its name.
This young family-run winery was born in 2006 with the help of their father Mariano when two brothers Angelo and Loris decided to continue the activity initiated in 1956 by their grandfather Angelo.
As they currently run the winery using their own grapes, Loris, with a degree in oenology, works in the cellar and is responsible for winemaking, while Angelo takes care of the accounts and customer relations. Mariano then, with a long-mature-acquired experience throughout the years, ensures cultivation in the vineyard.
A portion of about 18 hectares of the vineyard is cultivated from which all typical wines of the zone are produced, namely Amarone, Recioto, Valpolicella and Ripasso.
You are most welcomed to the warm atmosphere of our 1800 ancient wine home recently restructured. Therein you are treated to a good reception and a warm hospitality. Have a taste!


text:
Orlann Ombissa-Dzangue (* 26. Mai 1991 in Sens als Orlann Ombissa) ist eine französische Leichtathletin, die sich auf den Sprint spezialisiert hat.

Sportliche Laufbahn
Erste internationale Erfahrungen sammelte Orlann Ombissa-Dzangue im Jahr 2008, als sie bei den Juniorenweltmeisterschaften in Bydgoszcz mit der französischen 4-mal-100-Meter-Staffel in 45,02 s den fünften Platz belegte. Zwei Jahre darauf gelangte sie bei den Juniorenweltmeisterschaften im kanadischen Moncton im 100-Meter-Lauf bis in das Halbfinale und schied dort mit 12,01 s aus, während sie mit der Staffel mit 45,70 s den Finaleinzug verpasste. 2011 schied sie bei den U23-Europameisterschaften in Ostrava mit 12,02 s im Vorlauf aus und gewann mit der Staffel in 44,26 s die Silbermedaille hinter dem Team aus Russland. Daraufhin beendete sie vorläufig ihre aktive Karriere, kehrte aber im Jahr 2017 zur Leichtathletik zurück und nahm mit der französischen Staffel an den Weltmeisterschaften in London teil, bei denen sie mit 42,92 s aber den Finaleinzug verpasste. Im Jahr darauf siegte sie bei den Mittelmeerspielen in Tarragona in 11,29 s über 100 Meter und auch mit der Staffel gewann sie in 43,29 s die Goldmedaille. Anschließend belegte sie bei den Europameisterschaften in Berlin in 11,29 s den achten Platz über 100 Meter und erreichte mit der Staffel nach 43,10 s Rang fünf.

2019 scheiterte sie bei den Weltmeisterschaften in Doha mit 11,34 s in der ersten Runde und wurde mit der Staffel im Vorlauf disqualifiziert. Nach einem weiteren Jahr ohne Wettkämpfe startete sie 2021 im 60-Meter-Lauf bei den Halleneuropameisterschaften in Toruń und belegte dort in 7,23 s den vierten Platz. Anfang Mai verhalf sie bei den World Athletics Relays im polnischen Chorzów der französischen 4-mal-100-Meter-Staffel zum Finaleinzug. Im August erreichte sie dann mit der Staffel das Finale der Olympischen Spiele in Tokio und belegte dort in 42,89 s den siebten Platz.

2021 wurde Ombissa-Dzangue französische Meisterin im 100-Meter-Lauf.

Persönliche Bestleistungen
 100 Meter: 11,06 s (+1,5 m/s), 7. Juli 2018 in Albi
 60 Meter (Halle): 7,15 s, 27. Januar 2019 in Paris

Weblinks

100-Meter-Läufer (Frankreich)
4-mal-100-Meter-Staffel-Läufer (Frankreich)
Olympiateilnehmer (Frankreich)
Teilnehmer der Olympischen Sommerspiele 2020
Teilnehmer der Mittelmeerspiele 2018
Mittelmeerspielesieger (100-Meter-Lauf)
Mittelmeerspielesieger (4-mal-100-Meter-Staffel, Leichtathletik)
Französischer Meister (Leichtathletik)
Franzose
Geboren 1991
Frau

redpajama_set_name: RedPajamaWikipedia


text:
«Крізь тисячі лісів» — другий студійний альбом Octopus Kraft виданий 2016 року.

Опис
Черговому альбому колективу характерні різкі зміни ритму: чергування середньотемпових монотонних гітарних рифів з мажорними акордами у супроводі гроулу. У порівнянні з дебютником, котрий містив значний композиційний дисонанс, «Крізь Тисячі Лісів» відрізняється вираженою концепцією, яка посприяла єдиному стилю та манері виконання. Оформлення диску здійснила художниця та  тату Маша ABRA aka Abra Black. Офіційно презентовано 25 серпня у клубі «Underground».

Склад на момент запису

 Юрій Дубровський — гітара, вокал
 Андрій Чернов — гітара
 Сергій Володарський — ударні
 Дмитро Туманов — бас

Запрошені виконавці
 Мар'яна Жданюк — вокал («Ненависний», «Засинай»)

Примітки

Посилання
 Крізь тисячі лісів  в Encyclopaedia Metallum

Українськомовні музичні альбоми
Альбоми українських виконавців


Welcome to the Little Hearts Matter registration page.
Becoming a member or associate member of Little Hearts Matter could not be easier and is free to anyone seeking support and information because they are affected by a diagnosis of a single ventricle heart condition.
Full membership is open to adults with a single ventricle heart and to parents, and grandparents, of a child diagnosed as having a single ventricle heart, resident in the UK or Ireland.
Membership entitles you to access full support from the LHM team, regular newsletters and hard copies of written booklets;contact from regional supporters and invitations to national and regional events; and access to all open social media forums and membership of the closed Member only Facebook sites. It also entitles you to vote on annual accounts and the membership of the Board of Trustees.
Child and Young Person Membership is open to any child or young adult (under the age of 18) living with a single ventricle heart condition.
Membership entitles you to access support and information from the LHM youth and support team; to regular, age-appropriate newsletters, web and written information; as well as to invitations to regional and national events. When you reach 13 membership will entitle you to join in with youth social media forums.
Parents and grandparents of children with single ventricle hearts outside the UK.
Extended family members from within the UK and abroad, for example, aunts, uncles and siblings.
Health, education and social service professionals.
Associate membership enables you to seek support and information from the LHM team, to access website and email news and information, and to access public LHM forums.
If you would like to become a member we would love to hear from you. Select the section below that relates to you and click to find the appropriate registration form.
Young person with a single ventricle heart, under the age of 18 years .


Yes. These needles will work on all home sewing machines. One side of top of the needle is flat-- make sure it is inserted into the machine correctly.
Choose your needle size based on the type and weight of thread that is being used as well as the fabric that is being sewn. You want the needle to slide easily through the fabric without damaging the fibers while not creating too large of a hole. Each package of SCHMETZ needles has European and U.S. size equivalents written at the very bottom of the front of the plastic case. The first number is the European size designation. The number after the / is the US designation. The smaller the number the smaller the needle.
Leather needles have a a slightly sharp cutting point that are specifically engineered for leather and other heavy non-woven synthetics including imitation leather. These are all 5 packs of needles.
Quality thread and needles are well worth the money. They will make your sewing experience more enjoyable and more successful. If you can't remember when you last changed your needle, it's probably time. Make sure you have the proper size and type needle for your project. There are some guidelines in the FAQ's below. If you have other questions, let us know.


text:
I killed for principle not money, former mobster tells jurors in Boulis murder case
By Rafael Olmeda and Sun Sentinel
Defense lawyers David Bogenschutz, left, and Christopher Grillo talk during a break Wednesday, Oct. 16, in the Gus Boulis murder trial. (Rafael Olmeda)
A former mob henchman who pleaded guilty to two murders and admits participating in others told a Broward jury Wednesday that he was insulted when Anthony "Big Tony" Moscatiello offered him $100,000 to kill Konstantinos "Gus" Boulis.
It wasn't that the man identified to jurors as "Nick DiMaggio" had anything against taking a man's life, he said. He just found it distasteful to do it for money.
"I was highly insulted that the guy offered me money to kill somebody," said DiMaggio, whose real name is Peter "Bud" Zuccaro, a Gambino crime family associate who cut a deal with federal prosecutors in 2005 in exchange for his testimony in numerous criminal cases.
"In my prior life, you didn't kill people for money," he told jurors. "If it was principle, you killed them. If you killed people for money, it was cursed. I didn't kill people for money. Principle, yes; money, no."
Zuccaro testified under an unusual veil of secrecy — Broward Circuit Judge Ilona Holmes banned television cameras from pointing in his direction for the three hours he was on the stand. Courtroom spectators were ordered to shut off their cellphones. And, most unusual, jurors were not informed that he used an alias on the stand.
But he provided enough information during his testimony to reveal his identity.
The Sun Sentinel reported on Zuccaro as a potential witness in the Boulis case in 2011. Details on why prosecutors wanted him to testify with a fake name were not released to the public, but Holmes agreed with prosecutors over defense objections last week.
Zuccaro has testified under his own name in several high-profile cases in New York, criticizing the late mob boss John Gotti for attracting too much attention to Gambino family activities.
As DiMaggio, he told jurors that he mulled Moscatiello's offer in the fall of 2000 and discussed it with his lifelong friend John Gurino. Ultimately, he said, he turned Moscatiello down.
He didn't think much of it again until he received a news article reporting Boulis' death in the mail in Feb. 2001. The article was sent to him by Gurino, DiMaggio said.
Prosecutors believe Gurino shot Boulis on orders from Moscatiello on Feb. 6, 2001.
A few weeks later, DiMaggio said, Moscatiello told him the Boulis matter had been taken care of.
Defense lawyer David Bogenschutz wasted no time attacking DiMaggio's credibility, getting the witness to admit to a string of violent crimes dating back to the 1970s.
DiMaggio did not shy away from his past, and even sounded regretful that he never went through with one planned murder.
"When I was in that life, I was in that life thoroughly," he said. "I was no good. I was a killer. I was a drug dealer. I was a [truck] hijacker. I beat people half to death. I did a lot of bad things. A lot of bad things. Very bad things."
One thing he did not do was try to kill Boca Raton delicatessen owner Ralph Liotta. "In 2003, I wanted to kill him really bad," DiMaggio said.
Liotta is serving a 12-year manslaughter sentence for the 2003 shooting death of DiMaggio's good friend Gurino. Liotta is also listed as a potential witness in the Boulis murder trial.
DiMaggio's testimony did nothing to tie Moscatiello's co-defendant, Anthony "Little Tony" Ferrari, to the Boulis murder. Ferrari's lawyer, Christopher Grillo, cross-examined the witness for less than a minute before returning to his seat at the defense table.
Moscatiello and Ferrari each face the death penalty if convicted of murder, conspiracy and solicitation to commit murder.
Wednesday was the first day of testimony in a week, because of the recent illness of Bogenschutz. The trial is scheduled to resume Thursday.
raolmeda@tribune.com, 954-356-4457 or Twitter @SSCourts


Women in the Workplace, a recent study conducted by LeanIn.org and McKinsey & Company – building off of similar work done by the latter in 2012 – examines the current state of women in corporate America. Over 100 companies and nearly 30,000 employees participated. The survey results and accompanying data shed some light on the fact that women are still underrepresented at every level of corporate life, and the study goes a step further, examining the root causes of the problem. Let's take a closer look at a few of the key findings.
1. Women are more underrepresented the higher they climb.
The results show some improvement since 2012, but women are still underrepresented on every rung of the corporate ladder. Women make up 45 percent of entry-level positions (compared with 42 percent in 2012) but, at the most senior executive level, they account for just 17 percent (moving one percentage point in the right direction since 2012).
2. It's not that women don't want to lead, or that they're more interested in work-life balance than men.
One refrain that seems to run on a loop when it comes to women and work is that women are held back by their priorities. Or, more precisely, that women crave more work-life balance than men do on average – that they are more likely to put their families first, and that holds them back professionally. In fact, recent studies have found just the opposite is true, and the results of the 2015 Women In the Workplace study confirm this.
The data reveal that although women were less likely than men to aspire to top positions, especially once they were already far along in the ranks, men were more likely than women to cite the ability to balance work and family as their reason to not want the top jobs. Women on the other hand, whether they had children or not, cited "stress/pressure" as their number one concern. Across the board, parents were more likely to say they wanted to be promoted to a top position. In fact, mothers were 15 percent more interested in being a top executive than other women.
3. There is a lack of understanding about the problem.
Although 70 percent of men surveyed reported feeling that gender diversity was important, only 12 percent felt that women had fewer opportunities. Only a third of women felt that gender diversity was a top priority for their direct manager. Thirteen percent of men said that, actually, it's harder for men to advance, due to gender-diversity programs.
4. The responsibility gap at home persists.
Men who hold senior-level positions are five times more likely than women to have a stay-at-home partner, according to the study. At the executive level, women are 85 percent more likely than male execs to have a partner who works full time. Furthermore, at every level of corporate life, women are more than four times more likely to say they do more chores than men, and they are nine times more likely to say they are responsible for more childcare. The gap, overall, does seem to be improving in terms of general household responsibilities, but childcare is still falling, at an alarmingly disproportionate rate, to women.
Check out the full report for more information.
What do you think of the current reality for women working in the corporate world? We want to hear from you! Leave a comment or join the discussion on Twitter.
What Do the New FLSA Overtime Rules Mean to You?


text:
#GPnews: 'Brexit' could solve junior doctor contract dispute, suggests Tory MP
Struggling GP partners set to go salaried as trusts seize practice contracts
GP payment system to be reformed to end 'unacceptable' burden on practice time
NHS offers £45m for GPs to implement electronic consultations
17:20 This evening we are leading with comments made today by health secretary Jeremy Hunt in the House of Commons on tomorrow's planned all-out strike by junior doctors.
As a result of the strike action over the next two days, some GP practices in England will be extending their opening hours to help deal with extra demand resulting from, the health secretary has claimed.
Read Pulse's full story here.
16:05 The BMA's junior doctor committee are trying to 'bring down the Government and health secretary Jeremy Hunt,' a source has told the BBC, an accusation the BMA branded 'ridiculous'.
The BBC reports that a Government source alleged that the BMA have radicalised 'a generation of junior doctors' and stated that the two all-out strikes were aimed at 'toppling the government'.
Mr Hunt is expected to make a statement shortly in the House of Commons on the imposition of the junior doctor contract, and tomorrow's industrial action.
14:28 Although 99.7% of junior doctors are against changes due to be imposed in the junior doctor contract, just 78% intend to participate in the full walkout over the next two days.
But when asked to clarify why, it turned out less than 5% of those not striking intended to 'cross the picket lines', while the remainder were ineligible to strike either because they were not scheduled to work during those hours or were not on NHS contracts.
The statistics, unveiled in a survey carried out by Justice for Health, the junior doctor group which is suing Jeremy Hunt claiming the imposition is unlawful, also found that 98.8% of doctors feel confident that patients will be safe during the strike days.
12:20 Eurosceptic MP, and leader of the House of Commons, Chris Grayling has said the junior doctor dispute could be resolved by leaving the EU, reports The Telegraph.
He suggested that the Government could use 'savings' from EU contributions to continue to give annual pay rises to junior doctors – something that will be axed with the imposition of the new contract.
Mr Grayling said: 'Getting to a proper seven-day NHS could make a real difference to this country. It would be so much easier to pay for that change if we took back control of our £350m a week contribution to the EU and spent it on our priorities.
'For example, the money at the heart of the current doctors dispute is just 5% of our overall annual contribution. We shouldn't be spending billions on the EU that could make our NHS better and help deal with its current challenges.'
11:35 It's all about the junior doctors' contract row this morning, as they gear up to go on all-out strike on tomorrow and Wednesday.
Earlier this morning, a junior doctor announced his intention to resign live on TV, over the ongoing dispute about the new contract set to be imposed by the Government.
Dr Ben White told ITV's Good Morning Britain how junior doctors feel 'backed into a corner' – adding that how there was 'not a lot of sense coming from the Government's side of things'.
The all-out strike is due to start at 8am tomorrow, a move that NHS England said has already resulted in 125,000 cancelled operations and appointments.
Dr White expressed sympathy for the people affected by the strike but insisted that he had an obligation 'to fight on behalf of patients and fight for the NHS'.
11:05 Elsewhere this morning, a junior doctor has tweeted that while protesting outside the Department of Health's Richmond House HQ, health secretary Jeremy Hunt was driven just a few yards up the road from Downing Street to his office – to avoid having to face junior doctors, she claims.
Just seen @Jeremy_Hunt be driven in chauffeured car from no.10 across the road 50 yards to Dept Health to avoid us pic.twitter.com/PiIZbEBA53
— Roshana Mehdian (@RoshanaMN) April 25, 2016
9:30 Good morning and welcome to the live blog.
The BMA has not ruled out taking permanent industrial action over the ongoing dispute with the Government over the imposition of the new junior doctor contract.
The Guardian reports that leaked emails to HSJ from the BMA's junior doctor committee last week outlined the possible ways the action could be stepped up, including a 'permanent walkout, the mass resignation of trainees and a recommendation that doctors seek jobs outside the NHS'.
It comes as junior doctors prepare to hold an all-out strike on 26 and 27 April this week, in which they will not provide emergency care.
Got a story? Let us know by tweeting the hashtag #GPnews or emailing newsdesk@pulsetoday.co.uk
Anonymous | Other healthcare professional25 Apr 2016 11:23am
Well the sooner the better as only this will have any sort of impact on the situation.
This problems needs resolving before this autumn and now is the time before even more juniors dissapear down under/leave/resign/change careers/etc...
Unscepted | GP Partner25 Apr 2016 2:55pm
Politicians will exploit the Junior Doctor's predicament or any other situation to campaign for or against Brexit.
It would be naïve to think that EU had something to do with contract imposition.
Anonymous | Sessional/Locum GP25 Apr 2016 7:34pm
I have a feeling it'll have to be mass resignation, juniors are the only ones who have the guts necessary. This government has no intention of negotiation. They want to break Drs 'like they broke the miners' and ' like they broke the unions' . They really see it in that kind of way


text:
Sustainable NYC
These female-founded Brooklyn organizations are changing the face of sustainability
By Emily Shinde
Apr. 24, 2020 | Updated Jan. 3, 2021 at 12:45 pm
FABSCRAP is one of the innovative, women-founded organizations at the forefront of the sustainability movement in New York. Photo: FABCRAP
While we're unable to celebrate Earth Day at in-person events and discussions this year, we find it important to still be connected to our community and the local organizations doing work to fight climate change. We could argue that this time of solitude and reflection is most ideal for considering our impacts on Mother Earth, and how humans have fundamentally changed the makeup of the natural world, thus leading to a more vulnerable ecosystem susceptible to pandemics.
Rather than becoming discouraged, we want to focus on all of the inspiring work being done locally to change the conversation around climate change and sustainability. Specifically, women-owned companies with new models and ways of engaging the public on the topics of fashion sustainability and the people most affected by climate change. We've rounded up three local organizations that continue to produce thoughtful content during this time. We hope you follow their work, and become fans of them like we are!
Dominique Drakeford and Whitney McGuire of Sustainable Brooklyn
Drakeford and McGuire founded Sustainable Brooklyn a few years ago with the goal of bringing black and brown people to the forefront of the conversations about sustainability. Through programming, consultancy work, stakeholder partnerships, and education, they envision a more inclusive future by "fashionably bridging the gaps between sustainability and targeted communities." Their signature symposiums are meant to bring more people into the movement and elevate black and brown voices who are already doing the work.
With Drakeford and McGuire partnering to form the duo Sustainable Brooklyn, their hope is to shift Black Indigenous People of Color to the forefront of sustainability, as much of how we discuss fashion and wellness is framed from a colonial perspective. McGuire would love to see people awaken to the idea that Brooklyn is an ecosystem. "If we as a community are 50% insecure, then the other 50% who aren't should be aware of these statistics," she says. "Questions we can all ask ourselves include 'what is my role in the climate crisis?' and 'what can I do in my capacity to make some changes?'"
While the initiative of Sustainable Brooklyn is new, the founders drive home the point that the work is not. "For us, it's not a conversation, it is literally our heartbeat, our livelihood," says Drakeford. "Sustainability is this insane ecosystem, to food, fashion, it hits EVERYTHING that has to do with how we take up space."
Both Drakeford and McGuire hail from other U.S. cities, Oakland and Dayton respectively, but they've seen similarities between Brooklyn and the neighborhoods they grew up in, specifically white flight and the loss of resources with it. "The beginning of me realizing that we need to bring sustainability literacy to our community was when I saw what was happening in my city: industries closing and the end of white flight affecting my community, with it the closure of social services and essential gathering places," says McGuire. "We can't escape blackness. We can't escape the experience our community is having all over the world."
"We're just getting started in Brooklyn," says Drakeford. "We want to scale our work to other systems, densely black and brown populations that need political anchors."
But much of the way the public is engaged with conversations around sustainability primarily taps into a white demographic, and Sustainable Brooklyn wants to change that. They want to be intentional with how they're giving access to their events and spaces, and how they're sharing information. "A lot of the moment is predicated on access to social media or attending certain events. We really want to be more hands-on and on the ground in spaces where people don't have access to this conversation in a traditional way," says Drakeford.
They're also excited about the deeper connections they want to make through their work, from shifting curriculum in educational systems, building more community-led initiatives, finding ways to partner with people throughout the supply chain and people working in public policy, and engaging with waste management in some capacity.
While COVID-19 has obviously upended in-person experiences, for now, follow them on Instagram for announcements about their upcoming symposium events (like a hackathon!) and online conversations.
Céline Semaan of The Slow Factory
The Slow Factory Foundation is, in our opinion, a jack of all trades in the sustainability movement. As a non-profit working on environmental and social impact through education and action since 2013, The Slow Factory has been at the intersection of climate and culture, starting new conversations on many levels through partnerships with other nonprofits, academia, and global brands.
When asked what one of the top priorities is for The Slow Factory as of now, Semaan emphasized that education is power. "It empowers the public into informed action," said Semaan. "Making information accessible to the public contributes to shifting culture on critical topics such as climate change and social justice. It then can create pressure on policymakers and elected officials in addressing these issues at scale."
The Slow Factory's tagline has always been, 'Good for the Earth. Good for the People.' They embody that motto by taking a whole approach to sustainability, from hosting Study Halls accessible to the public to brand partnerships that change the conversation around climate change, like their recent Landfills as Museums initiative with Adidas and local leaders in waste management.
Semaan understands that there are different entry points to sustainability for people, but that a larger approach is necessary for change. The Slow Factory doesn't frame their work to be only about the supply chain of materials, or worker's rights, or as a plea to brands to be more transparent. As stated on their site, The Slow Factory takes a "holistic, human-centered approach."
"Like many individuals and groups around the globe, we're strategizing how to navigate our current reality during this pandemic so we can continue to be a resource for our audience," says Semaan.
"There are so many unknowns right now but we're working on some exciting projects," says Semaan. For example, this week we're launching a new interview series on Earth Day called SlowTalks where we'll be talking with different leaders about sustainability. The first one is with Amy Hall, Vice President of Social Consciousness at Eileen Fisher." Stay tuned for more conversations, partnerships, and published content from The Slow Factory by following them on Instagram.
Jessica Schreiber and Camille Tagle of FABSCRAP
FABSCRAP is another relatively new initiative that has revolutionized the conversation of fashion sustainability. Rather than approaching the issue from the individual consumer's perspective alone, founders Schreiber and Tagle joined forces to help large brands and companies reuse and recycle their unused fabrics.
With a storefront in Chelsea and a warehouse in Brooklyn, FABSCRAP has ramped up its capacity to work with 450 brands to recycle and redistribute hundreds of thousands of pounds of fabric each year.
"If we are all aware of what we throw away as consumers, we can then educate people about what that means for how much more commercial waste is being thrown out," says Schreiber. "For every pound of textiles we throw away as consumers, the fashion industry's upstream is throwing away forty pounds of waste. We want to ask businesses what options they have and what steps they're taking to fix that."
"The work we do at FABSCRAP with pre-consumer waste ties back to climate change. Keeping things out of a landfill ties back, and our goal is to prevent that in all ways related to textile waste," says Tagle. "It's been a bridge that I need to make sure everyone understands – the connection between climate change and textile waste."
FABSCRAP has found ways to start a conversation about sustainability at various points in the supply chain, from design students being educated on their power of persuasion in the industry to large brands recycling their deadstock fabrics at FABSCRAP's warehouse. "There is a value to things that people think are disposable," says Tagle."Until we worked with them, it was inconvenient for brands to find ways to handle their volume. And design schools used to have to deny donations because they didn't have anywhere to hold and take care of fabrics."
While things may feel on pause at the moment, it's incredible to see the impact FABSCRAP has and will continue to have on the fashion industry. "We ended 2019 with 425 brands using our service. In two years we quadrupled. I think it shows both the need and the growth from brands who are aware of their impact and a desire to make a difference," says Schreiber.
For now, FABSCRAP's storefront and warehouse are closed due to COVID-19, but they're holding plenty of online workshops, fundraising happy hours, and is shipping scraps from their online store. Follow along on Instagram to stay in the know.
Tags: Climate Change, Sustainable NYC, Textile Waste, Zero Waste


So...maybe you could split the skies tomorrow, Jesus?
I haven't always meant those words when I've sung them, you know. I meant the "my faith shall be sight" part, but it was the hastening that I wasn't too thrilled about.
I wouldn't have admitted it to anyone, especially not to Jesus Himself—not that He needed my admittance to know it was there—but deep down in the undercurrent of my subconscious, I wasn't too ready for Him to come back.
While I'm being honest, let me also just go ahead and state it wasn't for holy purposes either—like my desire to evangelize to the ends of the Earth. Truthfully, it was mainly because there was a lot of living that I still had left to do. I wanted to see U2 in concert, walk down an aisle in a flowing white dress, and live in Europe, among other things.
I would hear people talk about Heaven and Jesus' return with anticipation, and I would just remain quiet. I mean, I knew that one day when I died or Jesus came back (whichever occurred first), I would regret my longing for more Earthly time, but that would be later. Eventually. Not now.
Maybe it was my small town upbringing that left me more sheltered than I realized. Looking back, besides the discipline that resulted from my smart mouth, my childhood was relatively painless. I didn't even have to process the weight of a death of someone close to me until my junior year of high school when my grandfather died. And even then, he was 89 and had lived a very full life.
The shame that overwhelmed friends who were sexually abused.
The searing pain that made students turn to drugs, alcohol, or other forms of self-mutilation to cope.
The despair of orphans who were abandoned by their parents.
The dissolution of marriages and families I knew and loved.
My own personal bent toward sin and my labor toward freedom.
And as I struggled through tough conversations and prayed for answers, I began to realize what Horatio G. Spafford—a man who lost his son to illness and his 4 daughters on a sunken ship—might've meant by "Lord haste the day…".
He was no stranger to pain and suffering, but I don't think Spafford was writing about escape. I think he was reminding himself of hope. All of our pain cannot and will not be resolved this side of Heaven. But one day it will be made right.
My heart can't help but want for Him to "hasten the day" more than I used to. I long for Him to restore us, to make us whole again.
But, I still don't want it to happen just yet. The difference is now I don't want Him to tarry for my self-centered pursuits. Spafford and his wife went on to have more children and minister to the poor in Israel. Like I suspect was true for the their family, I look around at all of the pain and I'm even more burdened for people to know this Hope in which I have received. And I'm more burdened about my role in sharing that with them.
The difference is now when I hear someone like Josh Garrels singing: "Sing, Lord come soon," or "I'm holding on to to the hope that one day this could be made right…" I sing with him. And I mean it. Tears fill my eyes and I exhale.


text:
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namespace App;

use Illuminate\Database\Eloquent\Model;
use Illuminate\Database\Eloquent\SoftDeletes;

class ReviewRequest extends Model
{
    use SoftDeletes;

    protected $fillable = [
        'content_id', 'user_id', 'reviewed_count',
    ];

    protected $dates = ['deleted_at'];
}


Check out what the Tweeple are saying about the upcoming MDRT meeting.
The exciment is running high with the upcoming Million Dollar Round Table (MDRT) 2015 Annual Meeting in New Orleans, which begins Sunday, June 14. What are some of the conferences or speakers that you're looking forward to reading and hearing more about?
We will be covering the event live, so stay tuned for more updates.


text:
Considering the slope and distance, the R s values of (i) and (ii
Considering the slope and distance, the R s values of (i) and (ii) were calculated to be 263.07 × 10−3 and 327.54 × 10−3 Ω/sq, respectively. Meanwhile, the Au-coated silica sphere array could be expected to yield the efficient bending of ZnO nanorods in ZnO NRA-based NGs as shown in Figure 3b. The strain effects of different surfaces of (i) flat Au and (ii) rough Au on ZnO nanorods were analyzed
by the numerical calculation with a commercial software (COMSOL 3.2, stress–strain application mode). Herein, it was assumed that the ZnO nanorods with a size/height of 60 nm/1 μm were bent under an external pushing force of 0.3 kgf/cm [2], and the rough Au has grating structures with a radius of 120 nm, as estimated from the FE-SEM image (in Figure 2a (ii)), Palbociclib molecular weight for the diameter of Au-coated silica spheres. From the strain distributions of (i) and (ii), it is clear that the bending radius of ZnO nanorods increased more when a pushing force to the NG with roughened Au top electrode was applied. This can be explained by the fact that the curvature AZD6738 order of the surface further
transmitted the external force to the side of ZnO nanorods. On the contrary, the flat Au transmitted the pushing force to the even surface of ZnO nanorods. For the strain effect of rough Au on ZnO nanorods, it would enhance the performance of ZnO NRA-based NGs with compensation of the slightly increased R s of the Au-coated silica sphere array. Figure 3 Electrical characteristics and simulation results. (a) Measured I-V curves and (b) simulation results of the strain distributions of (i) the flat Au film on PET and (ii) the Au-coated silica sphere array on PET. Figure 4 shows (a) the schematic diagram of the ZnO NRA-based NG with the Au-coated silica sphere array as a top electrode, (b) FE-SEM image of the grown ZnO NRAs on ITO/PET using the ED method, and (c) photographic image of the fabricated sample. In order to fabricate the flexible ZnO NRA-based NG, ITO and Au were used as cathode and anode with PET substrates. The polydimethylsiloxane (PDMS), an elastic soft material, acts as the spacer between the ZnO NRAs and top electrode. This maintained the separation
under a leasing pushing force. For the preparation of PDMS, the mixture with base resin and curing agent (weight ratio = 10:1) was poured in a flat Niclosamide petri dish until the thickness reached approximately 8 mm, and cured at 75°C for 2 h. After that, PDMS with a size of 3 × 0.8 cm2 was cut and laminated on the exposed surface of ITO/PET (bottom part) as can be seen in Figure 4a. To fix definitely the PDMS between the top electrode and bottom part, a Kapton tape was used for the attachment. After pushing the ZnO NRA-based NG, the bent top electrode is recovered by separating it from ZnO NRAs for the next pushing. Thus, this repeated process enables the rough surface of the Au-coated silica sphere array to compress continuously the ZnO NRAs.
Incubation of wild-type cells in LB with the NO synthase (NOS) in
Incubation of wild-type cells in LB with the NO synthase (NOS) inhibitor L-NAME and of a mutant that lacked the nos gene decreased in both cases NO production to ~ 7% as compared to untreated wild-type cells (Figure 1C-E). In contrast, supplementing MSgg medium with the NOS inhibitor L-NAME and growing the nos mutant
INCB024360 in MSgg decreased NO production to only 85% and 80%, respectively, as compared to untreated wild-type cells (Figure 1E). Figure 1 Nitric-oxide-synthase (NOS)- derived NO formation by B. subtilis 3610. (A-D) Confocal laser scanning micrographs of cells grown in LB for 4 h at 37°C. Shown is the overlay of: gray – transmission and green – fluorescence of NO sensitive dye CuFL. (A) Wild-type without supplements, (B) supplemented with 100 μM c-PTIO (NO scavenger), (C) 100 μM L-NAME (NOS inhibitor), and (D) 3610Δnos. Scale bar is 5 μm. (E) Single-cell quantification of intracellular NO formation of cells grown in LB (gray bars) Selleckchem BYL719 and MSgg (white bars) using CuFL fluorescence intensity
(A.F.U. = Arbitrary Fluorescence Units). Error bars show standard error (N = 5). The data shows that B. subtilis uses NOS to produce NO in LB and indicates that NO production via NOS is low in MSgg. Furthermore, the NO scavenger c-PTIO effectively reduces intracellular NO and the NOS inhibitor L-NAME inhibits NO formation by NOS. Hence, both compounds are suitable tools to test the effect of NO and NOS-derived NO, respectively, on multicellular traits of B. subtilis. Moreover, the data indicates that B. subtilis produces significant amounts of NO with an alternative mechanism besides NOS when grown in MSgg. An alternative pathway of NO formation in B. subtilis could
be Branched chain aminotransferase the formation of NO as a by-product during the reduction of NO2 – to ammonium (NH4 +) by the NO2 – reductase NasDE [25]. Both LB (~35 μM) and MSgg (~ 5 μM) contained traces of oxidized inorganic nitrogen (NO3 – or NO2 -; NOx), which might be a sufficient source for low nanomolar concentrations of NO even if most NOx is reduced to NH4 +. Gusarov et al. [26] showed that NasDE actively reduces NOx in LB-cultures at the end of the stationary phase. However, NO production from ammonifying NO2 – reductases has so far only been reported for the ammonifying NO2 – -reductase Nrf of E. coli [27], but not for NasDE of B. subtilis. The potential ability of NasDE to generate NO may be an interesting subject for further research directed toward the understanding of how B. subtilis controls NO homeostasis under different environmental conditions. NO is not involved in biofilm formation of B. subtilis 3610 We tested the influence of NOS-derived NO and exogenously supplemented NO on biofilm formation of B. subtilis 3610 by monitoring the morphology of agar-grown colonies and the development of biofilms on the air-liquid interface (pellicles) in MSgg medium.
This led to the conclusion that both, wild type and the hOGG1Cys3
This led to the conclusion that both, wild type and the hOGG1Cys326 variant-encoded
proteins should be functional and probably do not exhibit significant differences in repair activities and hence the polymorphism at codon 326 would probably be neutral [53, 55]. Many epidemiological studies have investigated the association of the Ser 326 Cys polymorphism in the hOGG1 gene indicating an increased risk for head and neck cancers but the reports are conflicting [51, 56, 57]. Studies on the prevalence of this polymorphism in susceptibility to oesophageal cancer also show conflicting Vismodegib price results. Xing et al. [16] reported a positive association between the Cys 326 variant and oesophageal cancer risk in Asians population whereas
Tse et al. [58] reported no association in Caucasians. In the present study, the small number of samples did not allow us to make a comparison of the genotype distribution between cases and controls in order to determine whether the hOGG1 326Cys allele contributed to the risk of oesophageal cancer. However, the distribution of hOGG1 Ser 326 Cys genotype in our controls (0.44) is in agreement with the frequencies previously described in Caucasian population. This frequency is classically lower than that in Asians LY294002 purchase [21, 51, 59], suggesting that this allele may be differently distributed among ethnic groups and may not confer a particular susceptibility to oesophageal cancer in Caucasian population. The
allelic distribution of this polymorphism in our combined population followed Hardy-Weinberg equilibrium. Besides DNA repair activity, enzymes involved in the detoxification of xenobiotics such as glutathione S -transferases may influence the extent Glutathione peroxidase of oxidative damage in humans. We genotyped our study population for the GSTM1, GSTT1 and GSTP1 genes. Our results indicate no association between GSTM1 and GSTT1 null polymorphisms and 8-oxodG levels in DNA from PBMCs. On the other hand, we found a statistically significant association between GSTP1 Val/Val homozygote carriers and a high level of 8-oxodG (Figure 2). However, as no obvious relationship was found between the frequency of the Val allele (Val/Val and Ile/Val combined) and the level of 8-oxodG, we consider this result questionable. Indeed, correlation of GST polymorphisms with 8-oxodG levels in WBCs or lymphocytes varies with the context of exposure: polycyclic aromatic hydrocarbons [60, 61], benzene [62], fine particulate matters [63] and hyperbaric oxygen [64]. Conclusions In conclusion, although the power of our study is limited, it seems likely that vitamin levels in serum and polymorphisms in the hOGG1 or GST genes are not important modulators of 8-oxodG levels.
The effects of arginine supplementation
The effects of arginine supplementation Opaganib on performance are controversial. Approximately one-half of acute and chronic studies on arginine and exercise performance have found significant benefits with arginine supplementation, while the other one-half has found no significant benefits [179]. Moreover, Greer et al. [180] found that arginine supplementation significantly reduced muscular endurance by 2–4 repetitions on chin up and push up endurance tests. Based on these results, the authors of a recent review concluded that arginine supplementation had little impact on exercise performance
in healthy individuals [181]. Although the effects of arginine on blood flow, protein synthesis, and exercise performance require further investigation, dosages commonly consumed by athletes are well below the observed safe level of 20 g/d and do not appear to be harmful [182]. Citrulline malate Citrulline malate (CitM) has recently become a popular supplement among bodybuilders; however, there has been little scientific research in healthy humans with this compound. CitM is hypothesized to improve performance through three mechanisms: 1) citrulline is important part of
the urea cycle and may participate in ammonia clearance, 2) malate is a tricarboxylic acid cycle intermediate that may reduce lactic acid accumulation, and 3) citrulline can be converted to arginine; however, as discussed previously, arginine does not appear to have an ergogenic effect in young healthy athletes so it is unlikely CitM exerts an ergogenic effect through this mechanism [179, 183]. Supplementation Epigenetics Compound Library with CitM for 15 days has been shown to increase ATP production by 34% during exercise, increase the rate of phosphocreatine recovery after exercise by 20%, and reduce perceptions of fatigue [184]. Moreover, ingestion of 8 g CitM prior to a chest workout significantly increased Thymidylate synthase repetitions performed by approximately
53% and decreased soreness by 40% at 24 and 48 hours post-workout [183]. Furthermore, Stoppani et al. [173] in an abstract reported a 4 kg increase in lean mass, 2 kg decrease in body fat percentage, and a 6 kg increase in 10 repetition maximum bench press after consumption of a drink containing 14 g BCAA, glutamine, and CitM during workouts for eight weeks; although, it is not clear to what degree CitM contributed to the outcomes observed. However, not all studies have supported ergogenic effects of CitM. Sureda et al. [185] found no significant difference in race time when either 6 g CitM or a placebo were consumed prior to a 137 km cycling stage. Hickner et al. [186] found that treadmill time to exhaustion was significantly impaired, with the time taken to reach exhaustion occurring on average seven seconds earlier following CitM consumption. Additionally, the long-term safety of CitM is unknown. Therefore, based on the current literature a decision on the efficacy of CitM cannot be made.
33% or
3 3% pectin had a clear difference in their compos
3.3% pectin had a clear difference in their composition of cecal bacteria, which was illustrated by PCA (Figure 2). Figure 2 PCA analysis of samples from Experiment B. Principal Component Analysis of DGGE profiles of bacterial rRNA genes present in fecal samples from rat click here fed with control diet (red) or pectin diet (green), respectively. A: Pectin in diet constituted 3.3%. The amount of variability accounted for by Factor X is 25.5%, by Factor Y 19.6% and by Factor Z 13.8%. B: Pectin in diet constituted 0.33%. The amount of variability accounted for by Factor X is 36.4%, by Factor Y 22.1%, and by Factor Z 10.7%. Effect of short-term consumption of apple and apple pectin on the rat cecal environment (Experiment C) To further elucidate the observed effects of whole apples and apple pectin, three groups of eight rats were fed with either control diet, 10 g apples a day or 7% pectin for a period of four weeks. There was no significant effect on cecal BGL activity of the rats, but a significant (P < 0.01) increase in the activity of GUS was observed from 4.1 ± 1.2 U/g cecal content in control animals to 10.7 ± 5.6 U/g in animals fed with pectin (Table 2). In animals fed 7% pectin there was an increase (P < 0.01) in the production of cecal butyrate, check details a decrease in cecal pH (P < 0.01) and an increase in cecal
weight relative to total animal weight (P < 0.01). The apple fed rats also had a significant drop in cecal pH (P < 0.05) and increase in butyrate (P < 0.05), but no changes in GUS or cecal weight (Table 2). Table 2 Cecal parameters from experiment C. Dietary group Control 7% pectin 10 g apple Propionate (μmol/g cecal content) 6.8 ± 2.3 10.5 ± 4.4 10.2 ± 4.1 Butyrate (μmol/g cecal content) 3.7 ± 2.2 9.4 ± 3.1** 6.7 ± 4.5* Cecal pH 7.0 ± 0.1 6.6 ± 0.2** 6.8 ± 0.3* Relative cecum weight (g/kg b.w.) 12.3 ± 1.9 19.0 ± 5.2** 15.2 ± 5.4 GUS (U/g cecal content) 4.1 ± 1.2 10.7 ± 5.6** 5.9 ± 2.9 BGL (U/g cecal content) 3.5 ± 0.6 4.9 ± 1.8 3.8 ± aminophylline 1.8 The data are averages and standard deviations from eight animals in each group. * Asterisks indicate a significant difference from the control group; P < 0.05 (*) or P < 0.01 (**). U is defined as μmol/h. In the short-term experiment,
PCA of the universal DGGE profiles did not reveal an effect of apple consumption (data not shown), as was observed in the long-term trial (Experiment A). However, a marked effect of pectin consumption was observed (Figure 3). Sequencing of bands, which were present on the profiles from pectin-fed animals, but not on the control profiles revealed that these bands represented species belonging to the Gram-negative genus of Anaeroplasma, and the Gram-positive genera Anaerostipes and Roseburia, respectively. Similarly, it was found that bands present on the control profiles but absent on the profiles from pectin-fed rats represented Gram-negative Alistipes and Parabacteroides sp (Figure 3, Table 3).
J Immunol 1982,128(2):668–674 PubMed 68 Re F, Strominger JL: Tol
J Immunol 1982,128(2):668–674.PubMed 68. Re F, Strominger JL: Toll-like receptor 2 (TLR2) and TLR4 differentially activate human dendritic cells. J Biol Chem 2001,276(40):37692–37699.PubMedCrossRef Competing interests selleck chemicals llc The authors declare that they have no competing interests. Authors' contributions HRJ conceived of and performed most of the experimental work for the study and drafted the manuscript. JP participated in the bulk of the experimental
work. EAF participated in and assisted in design of the flow cytometric analyses. JEB and XRB created the transposon library and isolated the galU mutant strain of FTLVS. FR assisted in design of and performance of RNase protection and IL-1β measurements from infected cells in vitro. FDE performed the antimicrobial sensitivity assays. MAM oversaw the design and coordination of all studies, performed the statistical analyses, and helped to draft the manuscript. All authors have read and approved the final manuscript."
"Background The genus Bifidobacterium represents one of the most important bacterial group in human and animal feces [1–5]. This organism has stringent nutrient requirements and grows poorly outside of the animal gut,
making this bacterial group a potentially useful indicator of fecal pollution as previously described [6]. In addition, an advantage in using bifidobacteria instead of other fecal contamination indicators is the host specificity, human or animal, of some groups of Bifidobacterium species [3] contrary to coliforms, which are ubiquitous [7]. For www.selleckchem.com/products/sorafenib.html example, sorbitol-fermenting bifidobacteria are associated with human fecal pollution, while B. pseudolongum is predominant in several animal hosts SSR128129E and does not have been isolated from humans [3, 8, 9]. B. pseudolongum has been isolated in more than 80% of all bifidobacteria positive fecal samples from different animals (most were collected from cattle and swine) [10]. Less than 5% of these samples were positive for bifidobacteria of human origin. This suggests that this species could be an
interesting candidate for detection of animal fecal contamination. Several studies used bifidobacteria to track fecal contamination in surface water [11–13]. Beerens and coll [14] proposed to use bifidobacteria as fecal indicators in raw milk and raw milk cheese processes and molecular method versus culture-based method have been compared for detection of bifidobacteria in raw milk [15]. A PCR method based on the hsp60 gene, already sequenced in most Bifidobacterium species [16, 17] was developed for a rapid detection of bifidobacteria in a raw milk cheese process. A higher level of bifidobacteria was detected comparing to the level of E. coli suggesting that bifidobacteria could be a more convenient indicator. However, this method did not allow the identification of the bifidobacteria species.
Johnson TJ, Nolan LK: Pathogenomics of the virulence plasmids of
Johnson TJ, Nolan LK: Pathogenomics of the virulence plasmids of Escherichia coli . Microbiol Mol Biol Rev 2009,73(4):750–774.PubMedCentralPubMedCrossRef 11. Nicholls L, Grant TH, Robins-Browne RM: Identification of a novel genetic
locus that is required for in vitro adhesion of a clinical isolate of enterohaemorrhagic Escherichia coli to epithelial selleckchem cells. Mol Microbiol 2000,35(2):275–288.PubMedCrossRef 12. Paton AW, Srimanote P, Woodrow MC, Paton JC: Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans. Infect Immun 2001,69(11):6999–7009.PubMedCentralPubMedCrossRef 13. Tarr PI, Bilge SS, Vary JC Jr, Jelacic S, Habeeb RL, Ward TR, Baylor MR, Besser TE: Iha: a novel Escherichia coli O157:H7 adherence-conferring molecule encoded on a recently acquired chromosomal island of conserved structure. Infect Immun 2000,68(3):1400–1407.PubMedCentralPubMedCrossRef 14. Toma C, Martinez Espinosa E, Song T, Miliwebsky E, Chinen I, Iyoda S, Iwanaga M, Rivas M: Distribution of putative adhesins in different seropathotypes of Shiga toxin-producing Escherichia coli . J Clin Microbiol
HER2 inhibitor 2004,42(11):4937–4946.PubMedCentralPubMedCrossRef 15. Vu-Khac H, Holoda E, Pilipcinec E, Blanco M, Blanco JE, Dahbi G, Mora A, Lopez C, Gonzalez EA, Blanco J: Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia. Vet J 2007,174(1):176–187.PubMedCrossRef 16. Toledo A, Gomez D, Cruz C, Carreon R, Lopez J, Giono S, Castro AM: Prevalence of virulence genes in Escherichia coli strains isolated from piglets in the suckling and weaning period in Mexico. J Med Microbiol 2012,61(Pt 1):148–156.PubMedCrossRef 17. Smeds A, Pertovaara M, Timonen T, Pohjanvirta T, Pelkonen S, Palva A: Mapping the binding check details domain of the F18 fimbrial adhesin. Infect Immun 2003,71(4):2163–2172.PubMedCentralPubMedCrossRef 18. Nagy B, Fekete PZ: Enterotoxigenic Escherichia coli (ETEC) in farm animals. Vet Res 1999,30(2–3):259–284.PubMed 19. Sonntag AK, Bielaszewska
M, Mellmann A, Dierksen N, Schierack P, Wieler LH, Schmidt MA, Karch H: Shiga toxin 2e-producing Escherichia coli isolates from humans and pigs differ in their virulence profiles and interactions with intestinal epithelial cells. Appl Environ Microbiol 2005,71(12):8855–8863.PubMedCentralPubMedCrossRef 20. Prendergast DM, Lendrum L, Pearce R, Ball C, McLernon J, O'Grady D, Scott L, Fanning S, Egan J, Gutierrez M: Verocytotoxigenic Escherichia coli O157 in beef and sheep abattoirs in Ireland and characterisation of isolates by Pulsed-Field Gel Electrophoresis and Multi-Locus Variable Number of Tandem Repeat Analysis. Int J Food Microbiol 2011,144(3):519–527.PubMedCrossRef 21. Karmali MA, Gannon V, Sargeant JM: Verocytotoxin-producing Escherichia coli (VTEC). Vet Microbiol 2010,140(3–4):360–370.PubMedCrossRef 22.
Figure 4 Percentage deviations between experimental and predicted
Figure 4 Percentage deviations between experimental and predicted densities. Deviations between experimental density data (ρ exp) and predicted values (ρ pred) by Equation 4 vs. mass concentration
(wt.%) for ( a ) A-TiO2/EG and ( b ) R-TiO2/EG nanofluids. Isobaric thermal expansivity, α p , and isothermal compressibility, κ T , coefficients can be determined from specific volume correlations using their respective thermodynamic Afatinib molecular weight definitions according the following expressions: (5) (6) In Table 2, the values calculated for α p and κ T are reported for some temperatures and pressures for the base fluid (EG) and both nanofluids at two different concentrations (1.75 and 5.00 wt.%). The estimated uncertainties for α p and κ T are 4% and 2%, respectively. The α p values for both the base fluid and R-TiO2/EG and A-TiO2/EG nanofluids decrease when pressure rises (up to 9.8% for the base fluid) and increase with temperature (up to 6.6% for the base fluid). Concerning the concentration dependence, first, we have found that the α p values of nanofluids are very similar
to or lower than those of EG, achieving decreases up to 1.0% and 1.9% for A-TiO2/EG and R-TiO2/EG nanofluids, respectively. Metformin These results are opposite to those previously found by Nayak et al. [8, 9], reporting a significant increase in this property compared to the base fluid for water-based Al2O3, CuO, SiO2, and TiO2 nanofluids. It should be mentioned that Nayak et al. have determined the isobaric thermal expansivities by measuring the bulk variation with temperature for the samples in a glass flask with a long calibrate stem. Consequently, further studies about this property are still needed on EG- or water-based nanofluids. On the other hand, the κ T values of the studied samples do not exhibit evident concentration or nanocrystalline structure dependence (or Cyclooxygenase (COX) these differences are within the uncertainty). The κ T values decrease when the pressure rises and increase with the temperature along the isobars for both the
base fluid and nanofluid samples, as can be seen in Table 2. In order to compare the volumetric behavior of the nanofluids with the ideal fluid behavior, excess molar volumes, , were calculated [10, 38]. Figure 5 shows an expansive volumetric behavior for both A-TiO2/EG and R-TiO2/EG. This behavior has also been found for other pure EG-based nanofluids, and it is contrary to that presented by nanofluids which use water or EG + water as the base fluid [28]. Excess molar volumes for A-TiO2/EG increase slightly with nanoparticle concentration ranging from 0.03 up to 0.11 cm3 mol−1, which correspond to a variation in the molar volume between 3.3% and 14.3%. Concerning R-TiO2/EG, its behavior is closer to ideal, and it is almost concentration independent with a maximum variation in volume of 4.6%. No significant temperature or pressure dependences for this property were found.
Observations done at 200× magnification Figure 5 TUNEL assay (mi
Observations done at 200× magnification. Figure 5 TUNEL assay (microscopic) after 48 hours incubation of
MCF-7 against catechine treatment. A, B and C are untreated control; D, E and F treated with 150 μg/mL of catechine; G, H and I treated with 300 μg/mL of catechine. Red BAY 73-4506 solubility dmso fluorescence is due to Propedium Iodide staining and observed under green filter while green fluorescence is due to FITC staining and observed under blue filter. Bright field image (B, E and H) central row. Observations done at 200× magnification. Figure 6 TUNEL assay (microscopic) after 72 hours incubation of MCF-7 against catechine treatment. A, B and C are untreated control; D, E and F treated with 150 μg/mL of catechine; G, H and I treated with 300 μg/mL of catechine. Red fluorescence is due to Propedium Iodide staining and observed under green filter while green fluorescence is due to FITC staining and observed under blue filter. Bright field image (B, E and H) central row. Observations done at 200× magnification. Quantification of mRNA levels of apoptotic-related genes To investigate the molecular mechanism of CH-induced apoptosis in MCF-7
cells, the expression levels of several apoptosis-related genes were examined. The relative quantification of Caspase-3, -8, and -9 and Tp53 mRNA expression levels was performed learn more by SYBR Green-based quantitative real-time PCR (RT-PCR) using a 7500
Fast Real Time System (Applied Biosystems). Figures 7 to 10 summarize the gene expression changes of Caspase-3, -8, and -9 and p53. CH increased the transcripts of Caspase Bcl-w 3, -8, and -9, and p53 by several fold. The expression levels of these genes in MCF-7 cells treated with 150 μg/ml CH for 24 h increased by 5.81, 1.42, 3.29, and 2.68 fold, respectively, as compared to the levels in untreated control cells (Figure 7). Similarly, the expression levels of Caspase-3, – 8, and – 9 and p53 in MCF-7 cells treated with 300 μg/ml CH for 24 h increased by 7.09, 3.8, 478, and 4.82 fold, respectively, as compared to levels in untreated control cells (Figure 8). In a time-dependent manner, the expression levels of the apoptotis-related genes in MCF-7 cells treated with 150 or 300 μg/ml CH for 48 h increased when compared to the levels in untreated control cells (Figure 9 and 10). However, the expression levels of Caspase-3, -8, and -9 and p53 in MCF-7 cells treated with 300 μg/ml CH for 48 h markedly increased–40.52, 8.72, 20.26 and 10 fold–as compared to control untreated cells (Figure 10). Together, these data suggest that these caspases and p53 were induced by CH in a dose- and time-dependent manner. Figure 7 Comparision of chang in expression of apoptosis related genes as fold change (ratio of target:reference gene) in MCF-7 cells after 24 hours of exposure of 150 μg/mL of catechin.
The resultant pET21aac was transformed into the expression host E
The resultant pET21aac was transformed into the expression host E. coli BL21(DE3). One ml of cultured E. coli BL21 (pET21aac) (OD600 = 0.6) were induced by using 1.0 mM IPTG for 20
h at 20°C. The harvested cells were resuspended in 0.5 ml of 50 mM sodium phosphate (pH 7.0) and then broken by ultrasonification for 1 min (pulse on, 0.8 s; pulse off, 0.2 s) with a Sonicator® (Heat System, Taiwan). The total proteins were analysed by learn more 6% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). ESI-MS analysis To analyse the degradation products of C7-HSL that were digested by E. coli (pS3aac), electrospray ionization mass spectrometry (ESI-MS) was performed on a Q-Tof Ultima™ API equipped with a nano-spray Z-spray source (Micromass, UK). One ml of E. coli (pS3aac) cells (OD600 = 1.2) was washed three times and suspended in 1 ml of 100 mM sodium phosphate buffer (pH 7.0) containing either 0.5 mM C7-HSL or 10 mM ammonia acetate buffer (pH 7.0) containing 0.5 mM C7-HSL, and then each sample was incubated at 30°C for 1 h. The reaction mixtures were centrifuged at 13,000 rpm for 1 min and then the supernatants were collected as the analytic samples. The analytic sample with the sodium phosphate buffer was diluted 100-fold with 0.018% triethylamine (pH 7.0) containing
Proteasome inhibitor 40% acetonitrile and 10% methanol and was then ionised by positive-ion electrospray (ESI+-MS) to detect HSL. The analytic sample with the ammonia acetate buffer was diluted 10-fold with 50% methanol and then ionised Amino acid by negative-ion electrospray
(ESI–MS) to detect heptanoic acid. In order to analyse the degradation products of aculeacin A, i.e. palmatic acid, 40 μl of Aac-digested mixture (6 μg of aculeacin A and 7.2 μg of purified Aac in 10 mM ammonia acetate) was diluted with 40 μl of 50% acetonitrile containing 0.1% formic acid and then detected by ESI+-MS. In this study, we used the following condition for ESI-MS. Approximately 400 nl/min analyte flow rate was used with the Q-Tof instrument. The cone and capillary voltage was set to 135 V and 3.5 KV, respectively, and the source block and desolvation temperature was 80°C and 150°C, respectively. The range of m/z value was set to 50 ~500 since this was sufficient for all of degraded products. Data was analyzed by MassLynx 4.0 software (Micromass, UK). HSL-OPA assay for AHL-acylase activity A modified homoserine lactone-o-phthaldialdehyde (HSL-OPA) assay was used to quantify the AHL-acylase activity [13]. Seven AHLs (Fluka Ltd, SG, Switzerland) were used as substrates of AHL-acylase. Various AHL-degrading products were collected using the preparation method of the analytic sample in the sodium phosphate buffer, as described in ESI-MS analysis.