Head on over to Adventures in Mommy Land to find my guest post with instruction on how to make this cute Santa ornanment with your toddler.
The post actually went up yesterday but I spent all day with my mom and dad for their birthdays. Their birthdays are just one day away from each other and they are the same age. I was a little neglectful of my online life in favor of my "real" life for a day! The post is still there, though, and there are still 9 days left until Christmas! I'd love for you to go check it out!
I saw your post on hands on:as we grow. The ornament is so cute!
I hope you do decorate trees outside.
It really is very cute, and I think even a non-crafter like me could manage it!
I meant BORN, not more. Ugh.
What a cute little ornament! And hooray for taking real life over blogland! Happy Birthday to your parents! My parents are the same age, too, born 13 days apart.


I'm not going to lie. I loved my Camaro at first. It was fast. It was loud. It was beautiful. It was the embodiment of the North American automotive enthusiast's dreams. I'd always wanted a muscle car from the time I was little. I'd been sold a dream come true, and for a while, all was well.
Then reality started to set in. You see, on a regular public road, I could wind out the Camaro in exactly one situation: Highway ramps. On a ramp, you could floor it, and rocket onto the highway, having reached cruising speed eons before you had to merge. Why not from a stop light you ask? People don't realize this, but it's really not realistic to floor it off of stop lights. Once you're used to it, all that's really going through your head is clutch wear, syncro wear (a piece of a manual transmission), the gallon of fuel you just burned, the 1000 km of tire life you just took off your set of $3,000 max-performance summer tires, and the very real chance that there's an undercover a row back.
I ran out of money one month in June. I generally budget pretty well, so I did what any nerd would do. I did a postmortem on my finances. What had gone so horribly awry?
As I detailed my finances, I discovered the horrible truth: I'd managed to literally burn through $550 in gas that month. I was only supposed to be going through $150. This was appalling to me. It just didn't seem worth it. So I tried to figure out how often I got to wring out my muscle car, to see if it was worth it to me. I started journaling my opportunities to unleash the beast. Yes, I know, how nerdy that makes me sound. Trust me, you don't know the half of it.
The answer? About twice a week. Despite taking the highway to work every single day, twice a day, when I was going onto the highway, or coming off, there was inevitably someone in front of me, preventing me from playing with my magnificent toy. Enough was enough. It was time to look elsewhere. I had always planned on moving to an electric car, but every time I'd run the numbers, I'd found them just a bit too expensive.
That's right. Coming from someone who owned a muscle car, my little electric Smart is more fun to drive. Why is that? Remember earlier, I was complaining about all the reasons why you didn't want to floor your muscle car off a light? The cost, the wear to transmission components, the fuel burned, and the cops? None of that applies to electric cars. There's no complicated transmission, no syncros, and no clutch. Your fuel is so cheap you can't bring yourself to care about its cost, and if you floor it off a light, there's no telltale engine roar to alert the police of your misdeeds. The result? You can floor this thing off every light. My car has an eco-meter, to let you know how economically you're driving the car. It's designed so the average drive will score 50%, and it tries to coach you towards 100%. I have drives where the eco meter is solidly sub-20%, and I'm grinning ear to ear.
When I drove the Camaro, it always bothered me that I was ripping through all this fuel, with no further justification than that I enjoyed it. The Smart on the other hand is so eco-friendly it spends its free time hugging trees for you. That may have different value to different folks, so I can only tell you, it has value to me.
In addition to it's relatively low purchase price, thanks to lucrative government subsidies in my area, it costs me almost nothing to drive. It's about $1.02 to drive to work and back. Assuming there are 20 work days in the average month, my 'fuel bill' is coming in at around $20. Compare that to the $550 I burned through earlier. In addition, I had to take my car in for its regular yearly maintenance last week. 1 hour of labor later, they had run a computer diagnostic, checked the battery, topped up the washer fluid, and sent me on my way. Maintenance on this thing is cheap.
I left this one to last, because it's the most surprising to me. Every muscle car owner secretly believes that the car will attract attention to them. They're designed to be big, loud, bright, and gorgeous. No one is going to argue that muscle cars are subtle! Reality doesn't live up to the advertising though. During the entire time I owned the Camaro, not a single person came up to talk to me about the car, not a single person stopped me at a gas station, or rolled down a window at a light. The Smart on the other hand gets loads of attention. The second day after I took it home, I got stopped at a local restaurant for someone to ask me if it was a hybrid. I get people rolling down windows to ask me if it's 100% electric, or to comment on how quickly I pulled away at the last light. I recently did a road trip, and stopped at a charging station on the side of the road. I had people pulling off the highway to come talk to me! This was the attention that I was supposed to get from the muscle car, coming instead in the form of the eco-curious asking about my little Smart.
I thought that I'd dislike the perceived step down to a diminutive electric car. My friends told me I'd regret it, and that I'd miss the Camaro. The reality is that the fun, the cost, and the attention have made the car a joy to own. I know my experience is not unique, because I hear the same thing from other electric car owners on a regular basis. So take it from a reformed gear head: Don't be scared of electric cars. Go give one a chance. I promise you, you won't regret it.
Stephen Grinwis is an EV evangelist, and general automotive enthusiast. His engineering background means he tends to nerd out a bit on the numbers. He focuses primarily on battery technology, wind power, and electric vehicles. If you can't find him running the numbers, or writing, you might find him lifting weights somewhere!


text:
Chiefs' FB Anthony Sherman, WR Gehrig Dieter and OG Andrew Wylie Throw a Holiday Party for Gold Star Families
The event marked a continuation of the Chiefs' "4th Quarter" social justice program
Matt McMullen
Community & Digital Media Producer
They each came from a different walk of life, from grandparents to little kids, but they all shared one commonality that led to this moment.
These individuals all had a family member who made the ultimate sacrifice in the line of duty, and as they filed into a local arcade on Monday afternoon, three members of the Kansas City Chiefs sought to honor that sacrifice in person.
"The military is something that's close to my heart," said fullback Anthony Sherman." [Chiefs' Director of Community Outreach] Chuck Castellano mentioned to me that there were some Gold Star families that might enjoy a night out, and I said to sign me up. This is something to give back and to provide a bright spot in their day. I wouldn't miss it for the world."
Andrew Mather
Gold Star families are relatives of U.S. servicemen and women who were killed while in service to our country. It's a sacrifice that can make the holiday season a difficult time for those missing loved ones, prompting Sherman, along with wide receiver Gehrig Dieter and offensive guard Andrew Wylie, to throw a holiday party for the families complete with food, gifts and plenty of hugs.
"You want to do everything you can to help them through the holidays," Sherman said. "It's the time of year when families get together, and we want them to be a part of everything that we have going on [in our family]."
That support was certainly felt throughout the entirety of the event, from racing go karts to a gift presentation.
"It's made everyone's day," said David Bussinger, who lost his nephew in the line of duty. "It takes your mind off of things for a little while. My grandchildren have been so excited since we learned that this was going to happen. We certainly think about my nephew and remember that this was for him. It just brings the family together for such a wonderful time."
The event was made possible through the Chiefs' social justice program, which seeks to support individuals who face barriers to opportunity through focus areas meaningful to NFL players.
Several members of the Chiefs have participated in initiatives of their own doing throughout the season and it continued this week as Sherman, Dieter and Wylie spent some time with those who have sacrificed so much for our country.
"It's exciting that I can be the bright spot in someone's day for a couple hours," Sherman said. "I can give my time to someone that's given a lot more than just that."
Photo Gallery: Gold Star Family Holiday Party
Chiefs players have made a commitment to social justice initiatives in 2018, one of which is the military, and hosted a holiday party for Gold Star families. The families were be treated to holiday gifts, go karts, arcade games, and food and beverages.
Chiefs players hosted a holiday party for Gold Star families.
Chiefs Recognize Whiteman Air Force Base with This Week's Salute to Service Honors Presented by Crown Royal
Col. Jeffrey Schreiner, who is piloting the B-2 Spirit aircraft flying over Arrowhead Stadium on Sunday, has been a Chiefs' fan his whole life
Chiefs Recognized TSgt. Randy Miller as Last Week's Salute to Service Honoree Presented by Crown Royal
TSgt. Miller was recognized during last Sunday's game against the Chargers
Chiefs Recognize SMSgt. Jeffrey Watson as This Week's Salute to Service Honoree Presented by Crown Royal
SMSgt. Watson is set to retire from four decades of service next month
Chiefs to Open Team-Themed Fitness Center in Overland Park in Spring 2021
Chiefs Fit to open enrollment center in January
Chiefs Recognize Sgt. Caleb Ash as This Week's Salute to Service Honoree Presented by Crown Royal
Sgt Ash has been deployed three times and won the national sniper competition earlier this year
Chiefs and GEHA Present Local Family with an Unforgettable Holiday Surprise
The Chiefs and GEHA partnered to provide a local mother with some much-needed financial assistance this week
Cancer Survivor Roger McCush Named the Chiefs' "NFL Fan of the Year" Nominee
McCush is one of 32 nominees for the NFL "Fan of the Year" award
Chiefs Recognize Marine Veteran Eric Gormly as This Week's Salute to Service Honoree Presented by Crown Royal
Gormly served six years in the Marine Corps and now helps local veterans pursue a higher education
Chiefs Recognize Tribal Elder Pete Fee as This Week's Salute to Service Honoree Presented by Crown Royal
Fee, a member of the Iowa Tribe of Kansas and Nebraska, served in the Army National Guard, the Navy and the Air Force
Arrowhead Stadium is a Voting Location This Fall: Here's What to Know
Kansas City residents who live in Jackson County can cast their ballots at Arrowhead Stadium this fall
Chiefs Recognize Senior Airman Alexander Cavender as This Week's Salute to Service Honoree Presented by Crown Royal
SrA Cavender has played a significant role in the Air Force's handling of the COVID-19 pandemic
Patrick Mahomes Named Among "100 Most Influential People of 2020" by TIME Magazine
The reigning Super Bowl MVP continues to earn his place on the global stage

redpajama_set_name: RedPajamaCommonCrawl


Been reading heaps about 1g Home Button failures - How's yours?
Discussion in 'iPod touch' started by OrangeDog, Oct 20, 2008.
Been reading heaps about 1g Home Button failures, mine seems to be lower than the glass and it used to be exactly flush with it. Also it does seem to have the same tactile feel that it used to. It's like the spring is flattening out.
i dont think there is a spring in the home button, but mine is also not as flush as it was.
Spring or whatever it is.....it sure does weaken quickly.
Mine's fine? exactly the same as when I got it a few months ago as far as I can tell.
Mines fine. I don't use home button to power on, I use the power button to turn on and off and home to take me back to home. My home button still feels pretty good.
Had my 1G for about a year almost and he home button feels and looks the same as it did when I first got it, and I don't really go easy on the iPod.
I have had mine since day one and mine is perfectly fine. No problems what so ever.
Maybe the people having trouble with their iPod's home button should stop using a hammer to push the button!
Someone have any links to forums or articles on this subject? Not calling it bogus, just haven't seen much of anything on it.
After 6 months of heavy use, mine's no different than it was on day one. What is the nature of the failure?
mine seems to be lower than the glass and it used to be exactly flush with it.
I've got a 1G touch and the home button has been lower than the glass from new. My son's home button is also lower than the glass on his 1G touch.
On both touch the home button has never been flush with the glass.
And they are both working perfectly.
I am up to my third replacement 1g iPod Touch (32gig).
My home button failed within two months after purchasing it. I have had a couple more replacements since for other issues relating to the screen e.g bright pixels on refurb replacements but have had no more issues yet relating to the home button again.
So glad now to have paid the money for an extended apple care.


text:
Tips For Your Island Puzzle Adventure
Your Blog » Tips For Your Island Puzzle Adventure
Previous: Style, Beauty And Lifestyle
Next: How To Boost Your Credit Score
What Jonathan Blow (creator of Braid) has said about puzzle style is fascinating. He claims that when generating Braid, he did not attempt to make good puzzles. Alternatively he explored the space of things that could be carried out using the guidelines of the planet. Every single sufficiently fascinating thing he identified was packaged and presented to the player, in its most succinct kind. Another tremendous puzzle game is Braid, which perfectly illustrates this concept. In Braid you are introduced with a new time-based mechanic in every single planet - rewinding time, zones unaffected by your time skills, a shadow that seems after rewinding time, and so on.Fill-in-the-blank: A clue that consists of a blank where the answer goes. A single of the easiest varieties of clues to solve. Idea 90: Let players know that two objects need to be combined by creating them smell the exact same. WEINTRAUB: So there you have it, a sampling of recommendations and methods for cluing theme and fill entries. At the end of this article, you can see the total list of clues for the puzzle.As I wrote above, lots of them utilised to be acceptable. It is fairly ok to have a sliding block puzzle in a 15 year old game. But if you have been pondering of undertaking that right now, you ought to most likely think again. The mechanic is overused and played out, and so a lot of far better mechanics have been invented considering that sliding blocks had been well-liked.If you happen to be not seeking for the direct answers to puzzles and instead want to acquire a greater understanding of how to solve each sort, visit the Spoiler-Free of charge Tips and Puzzle Types page for a guide on the guidelines and principles of every single sort of puzzle.Make your way out of the cornfield and admire all the chirping chicks that stick to you. Climb the rope at the finish of the path and swing into the open window. Inside the barn, you'll uncover some machinery. Pull the starter by the wheel a couple occasions to get it running. Then head all the way to the left to open the door to let the chicks in. The aim is to get the box down from the rafters. With the chicks on your trail, run all the way to the proper and wait till they meet you there. In case you loved this informative article and you would want to receive details relating to one-time offer kindly visit the web page. Then run back to the lever. That lever will activate the blower, which sucks up the chicks and spits them at the box on the rafters, knocking it down to your level. Never be concerned, the chicks are all fine. Use the box to move to the next area.Kids can place their puzzle-solving abilities to the test with brain-busting puzzle books, games and quiz books from Usborne. The Saturday crossword is really the hardest puzzle of the week. Mondays have the most straightforward clues and Saturday clues are the hardest, or involve the most wordplay. Contrary to common belief, the Sunday puzzles are midweek difficulty, not the hardest. They are just bigger.By and massive, escape rooms are curated experiences. Just open a drawer - any drawer - in your home to see why it really is challenging for players to identify what's actually important. Get trivial. Since they're usually fairly simple and do not involve wordplay, trivia clues also could be relatively simple to answer. Plus, you can locate trivia answers in outside resources when you happen to be stuck.I think this explains the distinction in texture among puzzle games - but is broadly applicable to any game in which mechanics are learnt and mastered. Puzzle games merely one-time offer you this cycle as their explicit goal, and as such, it becomes all the a lot more apparent when it is absent.The unsolved loop problem can also be completed utilizing only three colours. In other words, employing the 14 tiles with only 3 of the colours make a shape with the longest 3 loops. Only the longest loop of each colour counts, and the aim is to make the sum of the 3 lengths as huge as possible. My very best solution had total length eight+eight+7=23, but I received a remedy by Neil 'Rourke with length 12+9+four=25. I don't know what the highest feasible total is.55 Handpicked Escape Area Puzzle Tips That Develop Joy & Terror. Light can be utilised as a tool to reveal or hide clues. It can also be utilised to develop atmosphere, supporting your theme and helping immerse your players in the atmosphere you have produced. Please leave a detailed description about the situation you are seeing. Incorporate issues like game name, how usually you can reproduce it, the flash player you are making use of, and uploading a screenshot to assist us resolve your issue faster.This tends to be fairly chaotic but very effective. Escape space games are won and lost primarily based on how swiftly two team members who found connected items can match them together. This consists of issues like a code and a important, a essential and a lock, or a pattern that seems the very same way in two different areas.The most useful two keys are A and S. At any time, press A to add all the numbers left in the game, and press S to multiply all the numbers left in the game. For example, if you have 8 eight 8 left, the solution is certainly 8 + eight + 8, please click the up coming post you only need to hit A on your keyboard. This is much more quickly than clicking 5 times with your mouse. Other keys are shown in the figure beneath. If you use a classic keyboard and has numpads, you can also use 4 for the upper left number, five for the upper appropriate, kristalmonaghan53.wikidot.com 1 for the reduce left and two for one-time offer - https://nikehyperchasesp.com/rodneyrickelblog54/rotieer-thoughts-bending-puzzle-game/ - the decrease right.


text:
babyTel Reviews and Plans
babyTel
Website: ca.babytel.net/en/business/overview/
Devices: Android, Blackberry, iOS, Windows & Mac
Coverage: AB, BC, MB, NB, NF, NS, ON, PEI, QC & SK
babyTEL is a Montreal-based VoIP service provider, providing both residential and commercial VoIP access throughout the United States and Canada. First launching in May 2004 as a Canada-only operator, babyTEL is believed to be now one of Canada's leading VoIP service providers. In February 2007, babyTEL also ventured south of the border by providing service in the U.S. market. Though worth mentioning, babyTEL has been in the telephony industry since 1991, making babyTEL a true experienced and seasoned veteran!
In GoneVoIP's babyTel Review, we found the Cloud PBX plans to have a good set of features for businesses such as:
Auto-Attendant
Voicemail-to-Email
It also one of the few VoIP providers to list Home Alarm support. The business plan also provides the integration with ROAMbaby; which is the babyTel smartphone and desktop app. When combined with the Business Phone the ROAMbaby app works as an extension giving the typical flexibility of carrying just one phone number.
Following are the current 3 plans babyTel is offering:
Lite Extension at $17 per month
Inc. 100 minutes of calling
All-In Extension at $27 per month
Inc. Unlimited incoming calls and Unlimited calls to Canada & the USA
All-In Extension with Phone at $27 per month (promo)
Same as above but includes a Yealink IP Phone
We found the rate for the monthly plan per user to be among the somewhat expensive in the market. While the plans say it's unlimited calling in the terms of service babyTel does state it's subject to fair usage; though it says it will compare use against others that have the same plan it does not report the limit in terms of minutes - a bit strange as the majority of providers nowadays do report it. Another odd fee babyTel as implemented is that if you want to use your own ATA they'll charge you a monthly fee; why would that be its a mystery. Finally, you also have to factor shipping costs, if you are buying a phone from them. The international calling rates from babyTel are good but not exceptional. When compared to other international long-distance providers such as G3 Telecom we found these are usually twice as expensive or more.
One of babyTEL's features is its single-line/multiple location capabilities, giving users the ability to route a single number to two or more phones that can be separated by a few feet or by thousands of miles. babyTEL is also a pioneer in social-networked VoIP having launched an early integrated telephone service for the Facebook platform called Telephone.
In all babyTel, Cloud PBX business phone solution does have very good features and seems good technology and support behind the sales offering. Though pricing practices and some lack of clarity in the services (how many minutes have unlimited plans?) make for an unappealing phone solution. The market has better offers out there.


The CANVIO AeroMobile by Toshiba is a wireless storage solution designed with mobile users in mind. The pocket sized device allows for wireless sharing and backing up of multimedia files between mobile devices as well as Mac and Windows PCs. You can access CANVIO's 128GB of storage via USB 3.0, a slot or its own wireless LAN network which allows you to transfer files without an internet connection. CANVIO is paired with Wireless SSD for Toshiba, a free mobile app that makes managing stored files convenient. Grab a CANVIO and preserve your multimedia files on-the-go.


text:
Dealer Audit
Funder Audit
Customer Login Secure File Upload
Digitisation of the Vehicle Audit Process
Boosting Growth Through Digitisation
Growth Through Digitisation of the Auto Finance Sector
Modern Dealer Audit Technology: the Evolution of Dealer Self Audits
Creating Your Stock Audit Plan: What to Check Before You Start
Motor industry
Finding Lost Revenue for Car Dealers
Results of 1000 Dealer Audits Cash is King, so the saying goes. In the automotive industry, this is especially true as cash flow management is one of the key challenges for dealers with literally millions tied up in vehicle inventory. The automotive industry is a very competitive and fast-paced environment. With tight profit margins, it's…
AI: Managing Data, Improving Business Intelligence
Artificial intelligence (AI) has become a common household term. We all know about Siri, the intelligent virtual assistant that comes with Apple products' operating system, but AI is much more than just her. From a business perspective, AI has many uses. From data analysis to automation, AI is rapidly being endorsed by businesses and is…
Now is the Time to Digitise
The current global context is pushing organisations to make better-informed, faster decisions to survive in a rapidly changing environment. As organisations begin to shift from the recovery phase of COVID-19 to the renewal, many are focused on what comes next and capitalising on the changes made to the business during the height of the crisis. One question…
5 Simple Ways to Improve Dealership Finances
Dealership finances can be complex and there is a lot to manage. In uncertain times, this is even more important and it's a challenging time for many but there are some simple things you can do to improve dealership finances. In this article we're going to share 5 simple ways that dealership CFOs can improve their…
Dealer Network: Simultaneous Group-wide Audit
When it comes to physical vehicle audits two of the biggest challenges are scale and speed. Audits need to be conducted and concluded quickly to be relevant but the bigger the scale the more challenging the process. We recently conducted a case study with a client that was avoiding conducting network stock audits as it…
WLTP: What is it all about?
WLTP (Worldwide Harmonized Light Vehicles Test Procedure) WLTP is a new, realistic measuring process for determining a car's fuel consumption, range and emissions. It is mandatory for all manufacturers and will be binding in all participating countries. However, while the name suggests it is a "worldwide" standard, no country in either North or South America is part…
What Impact will Artificial Intelligence have on Audits?
We are at an inflection point in the debate about what artificial intelligence (AI) means for industries and professions. With the critical mass of data now enough to feed the AI engine, its early applications are yielding some very interesting results. There are many truths and half-truths out there concerning the impact that AI will…
CheckVentory allows car dealers to self-audit (SAudit) more accurately
When most people see shiny new cars on a garage forecourt they don't know that the dealer doesn't own the cars. A financial institution or the distributor for the brand they represent does," says Adrian Walsh founder of CheckVentory, an automated stock checking system designed to replace manual inventory control in high value asset sectors…
Keeping stock pays dividends
Early career experiences often provide the seedbed of inspiration for future enterprise. In the case of Adrian Walsh, a combination of careers in the information technology and automative industries combined to provide the idea for CheckVentory, a new firm that recently won the 2015 PwC Docklands Innovation Award. While working as MD for one…
What's a Vehicle Identification Number?
What's Your Number? Most people don't realise that since the dawn of mass production, virtually every vehicle has had some form of a unique identifier stamped onto its body. These numbers helped manufacturers to link production to orders but numbering conventions varied by manufacturer. VINs were first used in 1954. From 1954 to 1981, there…
© 2021 CheckVentory Innovation Ltd. All Rights Reserved.


text:
These are either the worst, most horrible, senseless and stupidest movies I have seen, or just huge disappointments that didn't reach up to the hype that they created. And I am pretty sure this list is going to grow: …oh and there is no ranking, after all, they are all pretty bad.
Evil Dead:
The highly anticipated remake of the 80's horror flick, that had build up quite a fan community, failed in almost every angle: Bad storyline, horrible acting, and over-the top gore that was supposed to cover up the non-existing plot and lifeless characters. It might have been led the domestic box office for the opening weekend, but from there it just went down. Evil Dead has impressive gore effects, but you can forget the rest.
Prank:
What was meant to be a thought provoking Indie teenage drama turned out to be yet another weird hand-held camera movie that lacks not only in creativity but also in sense and meaning.
For more information about the movie click here
No One Lives:
Probably the weakest WWE release I have seen so far, even though it came with the most interesting storyline and really good actors. How did they mess this one up?
InAPPropriate Comedy:
This was not inappropriate, it was offensive in the worst kind of way. There is nothing positive I can say about this movie, it insulted my brain. Whoever helped creating this movie deserves a spot in the Hall of Shame.
Scary Movie 5:
Untalented writers, bad actors, and for being a parody it didn't have any memorable moments. I think I smiled twice, but that's about it. It's not worth taking anybody's time.
Spring Breakers:
Young chicks that run around in bikinis killing people, a Caucasian gangster that acts like he is some wannabe thug, wearing a grill and braids, dialogues that were exchanged with random pseudo poetry that doesn't make any sense… I know, it doesn't even sound bad, but it is. I had such high expectations for this movie, but it turned out to be a waste of time!
I am not even sure why this movie was made. It is completely meaningless, and it gives the two leading actresses a bad name. And this comes from a director who made movies like Scarface and Mission Impossible.
Compulsion:
The only thing i liked about this so-called thriller: it was colorful and had nice food-shots. But I can watch the Food Network for that.
Tyler Perry's Temptation – Confessions Of a Marriage Counselor:
I like Tyler Perry but this movie was a huge disappointment. The story had no depth, and even the actors didn't seem to know what their character's motivation was, so it ended up in a disaster.
Sharknado:
One good actor can't carry a whole tragedy of a movie. The subplot at the beginning wasn't connected to the story at all and the actors were highly unmotivated. Now they try to defend themselves by saying it was all meant to be funny. Oh well.


text:
Q: Keyboard and Mouse stop working after reboot I was testing my Virtualbox 6.0 on my Ubuntu 18.04 and I tried to boot Mac OS, Windows 7 and Windows 10 at the same time to see how it performs. However my Ubuntu froze and not responding anything, so I had to force it to reboot. After reboot, everything was fine before log into my home, but after that my mouse and keyboard no longer respond to anything, also I notice that seems like the system works, because I do see my Dropbox is logging in and it appears a system crash report, and after 5 mins the screen faded to black, so what can I do?
I tried to fix broken package in the grub but nothing helped.
Update after 5 mins
I tried to log into Ubuntu Wayland and this time it worked, however I have three displays, two connected to the graphic card and one on motherboard. After this incident the display on motherboard no longer is available. However, it is a black screen and only the mouse can be displayed. So I think there was a fundamental error of the system configuration. Do you have any idea how to automatically fix all configuration errors?
Also, if I go back to normal Ubuntu with three display on the keyboard and mouse still don't work

redpajama_set_name: RedPajamaStackExchange


Gastric cancer (GC) is a major health problem causing significant morbidity and mortality, ranking the third most common cause of cancer-related mortality worldwide (1, 2). Over the past decades, increasing evidence indicates that the pathogenetic mechanisms of cancer encompass tumor microenvironment (TME), emphasizing a tight correlation with its alterations for cancer initiation, progression and metastasis. The dynamic and mutualistic relationship between tumor and its TME is formed early in malignant growth and evolves throughout the life history of a tumor. TME is the primary location in which tumor cells and the host immune system interact. It is now widely accepted that the immune system can recognize and respond to tumor cells either naturally or following therapeutic intervention. The immune surveillance is considered to be an important host protection process to inhibit carcinogenesis and to maintain cellular homeostasis, while evading immune surveillance is a hallmark of biological capabilities in cancer development (3). The TME immune suppression is of the upmost importance for tumor cell survival, invasiveness and metastatic dissemination. Recently, new strategies for overcoming TEM immune suppression have led to the new and innovative cancer therapies that are considered amongst the medical breakthroughs.
As an important element of TME, human microbiota, particularly the gastrointestinal microbiota, has attracted increasing attention, as it can affect cancer growth and spread in many ways. Human microbiota has important effects on the host and that a balance of the microbiota is necessary for homeostasis. Owing to its ability to modulate host metabolism, inflammation and immunity, the human microbiota is involved in the initiation, progression and dissemination of various types of cancer both at the epithelial barriers and in sterile tissues (4–6). Growing evidence has revealed the pivotal role of the microbiota in educating and modulating the host local and distant immune system (4, 7, 8). Geva-Zatorsky et al. has shown that human gut microbiota comprises a treasure trove of immunomodulatory bacteria. With germ-free mice monocolonized with one commensal bacterial strain, they have confirmed that most these gut microbes exert several specialized, complementary, and redundant transcriptional and immunomodulatory effects by extensive, unsupervised immunophenotyping, and transcriptomics (8). The normal balanced microbiota has been proposed to have a crucial role in the establishment and maintenance of host immune homeostasis, in which the complexity of the microbial community elicits a comparably complex immunological response in the host. There is increasing evidence in animal and clinical studies that the gastrointestinal microbiota can markedly influence the clinical outcome of cancer immunotherapy, mainly mediated by shifting from immune suppression to antitumor immunity (9–11). Previous study indicated that commensal bacteria control cancer response to therapy by modulating TME, which mainly promoted an adaptive immune response against the tumors (12). So far, the gastrointestinal microbiota plays an important mechanistic role in antitumor immunity, which has implications for the use of immune checkpoint inhibitors to treat cancer.
Immune system dysfunction especially immune escape is critical for cancer occurrence and progression. Immunosuppressive cells, such as regulatory T cells (Tregs) and plasmacytoid dendritic cells (pDCs), play crucial roles in tumor immune escape (1, 13–15). The importance of forkhead box protein 3 (Foxp3) positive Tregs in mediating gastrointestinal immune homeostasis and promoting the establishment and maintenance of peripheral tolerance is evidenced in both animal and human experiments (16–18). However, in the context of cancer their role is more complex, and they are thought to contribute to the progress of many tumors (19). Although human T cells can transiently induce Foxp3 expression without acquisition of Treg function (20, 21), Ebert et al. has demonstrated that expression of Foxp3 by tumor cells is stable (22). Tregs accumulation at high density in various human carcinomas is generally associated with a poor prognosis, as expected from their capacity to inhibit antitumor immunity (23). pDCs are also one of the most important players in cancer immunity and has been identified in many solid malignant tumors (24, 25), which played an important tolerogenic role in tumor immunity. It has been shown that pDCs are recruited to solid tumor tissues and are also associated with poor clinical outcome (26, 27). Specifically, pDCs can effectively generate Tregs from naive CD4+ T cells, which contribute to tumor immune escape (28). Our previous study has found that Tregs and pDCs together in tumoral tissues contribute to the tumor immune escape in patients with GC (29). As mentioned above, we have learned that the gastrointestinal microbiota is essential regulator of mucosal immunity in the stomach. Specific members of the commensal microbiota, such as the genus Clostridium and capsular polysaccharide A (PSA)-producing Bacteroides fragilis, are potent inducers of Foxp3+Tregs (30, 31). By comparing pDCs in germ-free and specific pathogen-free mice, Swiecki et al. has found that the microbiota may promote egress of pDCs from bone marrow, which is required for homeostatic pDCs distribution (32). However, most of these studies that focused on the interplay between microbiota and the mucosal immunity were based on the intestinal environment, while these interactions in stomach were relatively understudied. How and whether the gastric microbiota in tumor and its adjacent tissues was associated with the alterations of Tregs and pDCs was still unclear. To address this issue, we analyzed the Tregs and pDCs in tumor and tumor-free tissues from the same GC patients by immunohistochemistry, and compared its gastric mucosal microbiota from normal, peritumoral and tumoral tissues by high-throughput sequencing technique. Then we explored the correlations between gastric microbiota and the two immunosuppressive cells. Our present study will provide new insights into the relationship between gastric microbiota and the tumor immunity, which will help to establish new strategies in antitumor therapy targeting on gastric microbiota.
Our present study enrolled 64 GC patients without preoperative chemotherapy from April 2014 to May 2017 from our hospital. Stomach tissues were obtained from patients with primary GC who accepted gastrectomy. The tumor and tumor-free tissues were collected and confirmed by pathological diagnosis. The tumor and tumor-free (2–5 cm adjacent to the cancer tissue, Peritumor; >5 cm adjacent to the cancer tissue, Normal) tissues were collected, which were confirmed by pathological diagnosis (Table 1). The clinical and pathological staging were based on 7th edition American Joint Committee on Cancer (AJCC) cancer staging manual of GC TNM Staging (33). Finally, 59 tumoral tissues, 61 peritumoral tissues, and 60 normal tissues were selected for microbiota analysis, which were based on DNA amount and quality appropriate for 16S rRNA gene amplification and sequence analysis. The immunosuppressive cells such as Tregs and pDCs in gastric tissues were analyzed using immunohistochemistry. The following criteria were used to exclude subjects: body mass index (BMI = weight in kilograms divided by the height in meters squared) >30; use of antibiotics, probiotics, prebiotics, or synbiotics in the previous month; preoperative chemotherapy, radiotherapy, or other biological treatment before gastrectomy. The fresh stomach tissues were obtained from surgery and stored at liquid nitrogen immediately until further use.
Table 1. Clinical parameters of the patients*.
Blood dendritic cell antigen-2 (BDCA2) and Foxp3 were selected as cell markers of pDCs and Tregs, respectively. The expression of these immunosuppressive proteins in tissues was evaluated via immunohistochemical analysis. Serial 5-μm frozen sections were used in this study. The details of the IHC procedure were performed as described in our previous studies (29, 34). Commercially available primary antibodies were used according to the manufacturer's instructions (mouse anti-BDCA2, 1:800 dilution, MACS, CA; mouse anti-Foxp3, 1:200 dilution, Abcam, Cambridge, MA, USA). Negative control staining was carried out with cold PBS in place of primary antibody. Numbers of BDCA2+pDCs and Foxp3+Tregs in each of 5 high-power fields (magnification 400 ×) were counted by two blinded, independent researchers using a microscope (objective lens, 40 ×; ocular lens, 10 ×; BX41 microscope, Olympus, Japan).
Gastric mucosal bacterial genomic DNA was extracted from tissue samples by using the QIAamp DNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions with minor modifications (35). Amplicon libraries were constructed with Illumina sequencing-compatible and barcode-indexed bacterial PCR primers 319F/806R, which target the V3-V4 regions of 16S rRNA gene (36). All PCR reactions were performed with KAPA HiFi HotStart ReadyMix using the manufacturer's protocol (KAPA Biosystems) and approximately 50 ng of extracted DNA per reaction. Thermocycling conditions were set at 95°C for 1 min, 55°C for 1 min, then 72°C for 1 min for 30 cycles, followed by a final extension at 72°C for 5 min. All PCR reactions were performed in 50 μl triplicates and combined after PCR. The amplicon library was prepared using a TruSeqTM DNA sample preparation kit (Illumina Inc, San Diego, CA, USA). Prior to sequencing, the DNA concentration of each PCR product was extracted with the MiniElute® Gel Extraction Kit (QIAGEN) and quantified on a NanoDrop ND-1000 spectrophotometer (Thermo Electron Corporation) and Qubit 2.0 Fluorometer (Invitrogen). The purified amplicons were then pooled in equimolar concentrations and the final concentration of the library was determined by Qubit (Invitrogen). Negative DNA extraction controls (lysis buffer and kit reagents only) were amplified and sequenced as contamination controls. Sequencing was performed on a MiSeq instrument (Illumina) using a 300 × 2 V3 kit together with PhiX Control V3 (Illumina).
The 16S rRNA gene sequence data set generated from the MiSeq run were first merged and demultiplexed into per samples using the QIIME version 1.9.0 with default parameters (37). Chimera sequences were detected and removed using the USEARCH software based on the UCHIME algorithm (38). Open-reference operational taxonomic unit (OTU) pick was then performed with USEARCH V7 referenced against Greengenes database version 13.8 at 97% sequence similarity (39, 40). OTUs with a number of sequences <0.005% of the total number of sequences were discarded as recommended (41). The result was an OTU table, which could be used for subsequent downstream analysis.
For taxonomic assignment, the most abundant sequences were chosen as the representative sequences of corresponding OTUs. Taxonomic assignment of individual datasets were classified against the Greengenes database version 13.8 using both RDP classifier and UCLUST version 1.2.22 methods implemented in QIIME (39, 42). Any sequences that were identified as members of Eukarya, Archaea, Mitochondria, Chloroplasts and Cyanobacteria lineages, were removed. Alpha diversity was calculated with QIIME software with Python scripts base on the sequence similarity at 97% level, including index of observed species (OTU number), abundance-based coverage estimator (ACE), Chao1 estimator, Shannon, Simpson, Evenness and PD whole tree. Sequence coverage was assessed in mothur by rarefaction curves and Good's coverage (43, 44). Beta diversity was measured by unweighted and weighted UniFrac distance calculated with 10 times of subsampling by QIIME. These distances were visualized by principal coordinate analysis (PCoA) (45). Hierarchical clustering was performed and heatmap was generated using a Spearman's rank correlation coefficient as a distance measure and a customized script developed in the R statistical package. The output file was further analyzed using Statistical Analysis of Metagenomic Profiles software package (STAMP) version 2.1.3 (46).
For the predictive functional analyses, PiCRUSt software package version 1.0.0 was used to identify predicted gene families and associated pathways from inferred metagenomes of taxa of interest identified from the compositional analyses, which was based on the fact that phylogeny and function are closely linked (47). Predicted functional genes were categorized into Kyoto Encyclopedia of Genes and Genome (KEGG) orthology (KO), and compared across patient groups using STAMP at KEGG level 3. Pathways and enzymes were assigned using KEGG database options built into the pipeline. The pathways that were non-prokaryotic, had fewer than 2 sequences in each cohort, or had a difference in mean proportions <0.1% were excluded from analysis. The characterization of microorganismal features differentiating the gastric microbiota was performed using the linear discriminant analysis (LDA) effect size (LEfSe) method (http://huttenhower.sph.harvard.edu/lefse/) for biomarker discovery, which emphasizes both statistical significance and biological relevance (48). With a normalized relative abundance matrix, LEfSe uses the Kruskal-Wallis rank sum test to detect features with significantly different abundances between assigned taxa and performs LDA to estimate the effect size of each feature. A significant alpha at 0.05 and an effect size threshold of 2 were used for all biomarkers discussed in this study.
For continuous variables, independent t-test, White's non-parametric t-test, and Mann-Whitney U-test were applied. For categorical variables between groups, Pearson chi-square or Fisher's exact test was used, depending on assumption validity. For taxon among subgroups, ANOVA test was applied (Tukey-Kramer was used in post-hoc test, Effect size was Eta-squared). For correlation analyses, Spearman's rank correlation test was used. False-discovery rate (FDR) was calculated according to Benjamini-Hochberg, FDR-corrected p-values were denoted as QFDR and was used when performing all untargeted screening analyses of different taxa. Statistical analysis was performed using the SPSS V19.0 (SPSS Inc., Chicago, IL) and STAMP V2.1.3 (46). Correlations between immunosuppressive cells and key functional bacteria were assessed by calculating the Pearson correlation coefficient (r). GraphPad Prism version 6.0 (San Diego, CA) was used for preparation of graphs. All tests of significance were two sided, and p < 0.05 or corrected p < 0.05 was considered statistically significant.
The sequence data from this study were deposited in the GenBank Sequence Read Archive with the accession number SRP172818.
BDCA2+pDCs and Foxp3+Tregs were two major immunosuppressive cells in TME. BDCA2+pDCs are a relatively rare DC subset in tissues, while Foxp3 is the best-known marker for evaluating real Tregs that have a suppressive function. Immunohistochemical staining of BDCA2 revealed that the absolute numbers of pDCs were present in stomach peritumoral and tumoral tissues of GC patients, and Foxp3 indicated that Tregs were also increased than those in normal tissues (Figure 1A). We observed that increased number of BDCA2+pDCs in peritumoral (11.68 [range, 1.60–27.60], p < 0.001) and tumoral tissues (9.51 [range, 0–30.20], p < 0.001), when compared with the normal tissues (4.14 [range, 0–24.75]). We also found that the numbers of BDCA2+pDCs in tumoral tissues were significantly lower than that in peritumoral tissues (p < 0.05). In addition, the number of Foxp3+ Tregs in tumoral tissues (9.12 [range, 1.00–28.60]) were higher than that in peritumoral tissues (7.68 [range, 1.00–15.40], p < 0.001), and three times that in normal gastric mucosa (2.83 [range, 0.00–14.80], p < 0.001) (Figure 1B). We found that the prevalence of pDCs was significantly associated with that of Tregs in gastric tissues (Figure 1C; r = 0.287, p = 0.000). These results indicated that increased numbers of pDCs and Tregs in stomach tissues of GC patients might contribute to the TME immune suppression of GC.
Figure 1. Increased BDCA2+pDCs and Foxp3+Tregs in tumoral and peritumoral tissues. Representative immunohistochemical (IHC) staining of BDCA2 and Foxp3 in tumoral, peritumoral, and normal gastric tissue are shown (magnification, × 400) (A). Box plot set showing the BDCA2+pDCs and Foxp3+Treg cell level in different tissues. Independent t-tests were used to analyze variation among the three stomach microhabitats. #p < 0.01 when compared normal group with peritumoral and tumoral groups; *p < 0.05 when compared peritumoral group with tumoral groups (B). The presence of BDCA2+pDCs was positively correlated with the increased number of Foxp3+Tregs in stomach tissues (C).
With MiSeq platform, totally 10,099,372 high-quality reads with an average of 56,107 reads per sample were used for following analysis, with 3,283,689 reads in normal group, 3,591,578 reads in peritumoral group and 3,224,105 reads in tumoral group, respectively. The Good's coverage was 99.98%, indicating that almost all bacteria in the stomach have been obtained. The diversity indices such as Shannon and Simpson were significantly increased in peritumoral group, when compared with normal and tumoral groups (Figures 2A,B; p < 0.05), while the richness indices such as PD Whole tree but not observed species was significantly decreased in peritumoral and tumoral groups when compared with normal group (Figures 2C,D; p < 0.05). However, PCoA based on Bray-Curtis distance could not separate the three groups into different clusters (Figure 2E).
Figure 2. The diversity and richness of the gastric microbiota in different stomach microhabitats. Diversity indices such as Shannon (A) and Simpson (B), and richness indices, such as PD whole tree (C) and observed species (OTU number, D), were used to evaluate the overall structure of the gastric microbiota in the three stomach microhabitats. The data are presented as the mean ± standard deviation. Unpaired t-tests (two-tailed) were used to analyze variation among the three stomach microhabitats. Principal coordinate analysis (PCoA) plots of individual gastric mucosal microbiota based on Bray-Curtis distance (E). Each symbol represents a sample. *p < 0.05; NS, no significant.
LEfSe has found that Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Acidobacteria, and Fusobacteria were the dominant phyla in stomach. Our present data indicated that Proteobacteria was enriched in peritumoral group, while Firmicutes and Fusobacteria were enriched in tumoral group (p < 0.05). However, the abundant phyla, Bacteroidetes, and Actinobacteria, were not changed significantly among the three groups. In present study, we found that the composition of the gastric microbiota in tumoral group changed dramatically. At the genus level, we found that the only several abundant genera such as Halomonas, Shewanella, Enterococcus, and Brevundimonas were increased significantly and Legionella was obviously decreased in peritumoral group when compared with normal group (Figure 3; LDA score>2; p < 0.05). However, more abundant genera such as Streptococcus, Peptostreptococcus, Lactobacillus, Bifidobacterium, Neisseria, Veillonella, and Shewanella were increased, while Staphylococcus and Corynebacterium were decreased significantly in tumoral group when compared with the normal group. We also found that Enterococcus, Selenomonas, Halomonas, Bifidobacterium, Dialister, Roseburia, and Staphylococcus were increased significantly and Lactobacillus, Shewanella, and Megamonas were decreased in tumoral group when compared with peritumoral group. Interestingly, the GC associated pathogen, Helicobacter pylori (HP), was increased significantly in peritumoral group but not in normal and tumoral groups (p < 0.05). However, microbial biomarkers discovery using LEfSe found that the LDA score value was not higher than 2 fold for HP in our present study.
Figure 3. Different bacterial taxa among the three stomach microhabitats. LEfSe identifies the taxa with the greatest differences in abundance among the three stomach microhabitats. Only the taxa meeting a significant LDA threshold value of >2 are shown.
We explored potential correlations between the gastric mucosal bacterial populations and two major immunosuppressive cells in TME. With Pearson's correlation analysis, we found that Stenotrophomonas, Acinetobacter, Gemella, Neisseria, Aquabacterium, Haemophilus, Novosphingobium, Streptococcus, Massilia, Gemmiger, Chryseobacterium, and Brevundimonas were positively correlated with BDCA2+pDCs, while Comamonas and Brevibacterium were negatively correlated with BDCA2+pDCs in the Heatmap (p < 0.05; Figure 4). In addition, the genera of Streptococcus, Massilia, Oribacterium, Campylobacter, Selenomonas, Dialister, Photobacterium, and Fusobacterium were positively correlated with Foxp3+ Tregs, while Rhizobium, Gaiella, Cupriavidus, Faecalibacterium, and Dolosigranulum were negatively correlated with Foxp3+ Tregs (p < 0.05). Notably, most of these bacteria that significantly correlated with immunosuppressive cells in TME were non-abundant bacteria. In details, Stenotrophomonas was positively correlated with BDCA2+pDCs (r = 0.348; p = 0.000) and Comamonas was negatively correlated with BDCA2+pDCs (r = −0.154; p = 0.039), while Selenomonas was positively correlated with Foxp3+Tregs (r = 0.275; p = 0.000) and Gaiella was negatively correlated with Foxp3+Tregs (r = −0.163; p = 0.029). However, no significant correlations were observed between Helicobacter and the two major immunosuppressive cells in TME (p > 0.05). These correlations between the gastric mucosal bacteria and the two major immunosuppressive cells might contribute to the immune system dysfunction in TME.
Figure 4. Correlations between gastric microbiota and immunosuppressive cells in the TME. The pearson's correlation between gastric mucosal microbiota and two major immunosuppressive cells such as BDCA2+pDCs and Foxp3+Tregs in the TME was shown in the heatmap. The four genera such as Stenotrophomonas, Comamonas, Selenomonas, and Gaiella were shown their correlation with BDCA2+pDCs and Foxp3+Tregs in details. *p < 0.05; **p < 0.01.
The functional content of the gastric microbiota was predicted by PiCRUSt based on closed-reference OTU picking. We utilized STAMP (statistical analysis of taxonomic and functional profiles) to analyze differences at the KEGG level 3, a database for understanding metabolic functions, and at the gene level (Welch's t-test, Benjamini-Hochberg adjustment). In our present study, there were greater gastric microbiota associated KEGG gene function changes in tumoral group than those in normal and peritumoral groups, which was similar with the changes of gastric microbiota mentioned above. At KEGG level 3 that included 328 KEGG pathways, we identified eight different KEGG pathways that were significantly different between normal and peritumoral groups, 15 different KEGG pathways that were significantly different between peritumoral and tumoral groups, and 25 different KEGG pathways that were significantly different between normal and tumoral groups (QFDR < 0.05, Figure 5). These differential KEGG pathways mainly belonged to metabolism-related pathways, environmental information processing pathways, cellular processing pathways, genetic information processing pathways, human diseases-related pathways and organismal systems-related pathways. Nine and nineteen differential KEGG pathways predicted to be significantly upregulated in tumoral group, while 6 and 11 differential KEGG pathways were significantly downregulated when compared with peritumoral and normal groups, respectively. Several metabolism-related pathways were upregulated simultaneously in both peritumoral and tumoral groups, which might contribute to the GC TME changes.
Figure 5. PiCRUSt-based gastric microbiome study in the three different stomach microhabitats. The different bacterial functions in the three stomach microhabitats were evaluated between each other based on two-sided Welch's t-test. Comparisons among the three stomach microhabitats for each KEGG pathway at level 3 were shown by percentage. The Benjamini–Hochberg method was used for multiple testing correction based on the false discovery rate (FDR) by STAMP.
Growing evidence indicates that complex interactions among cells and their microenvironment is critical for both normal tissue homeostasis and tumor growth. TME has many components such as cells, soluble factors, signaling molecules, extracellular matrix and mechanical cues that can promote neoplastic transformation, support tumor growth and invasion, protect the tumor from host immunity, foster therapeutic resistance, and provide niches for dormant metastases to thrive (49). Although the importance of microenvironmental alterations in tumor development is recognized, the molecular mechanisms underlying these changes are only now beginning to be understood. Recently, human microbiota that thrive on and within the human habitats has been considered as one of the important elements of TME, which have been implicated in the development of some cancers including GC. Mounting evidence indicates that the gut microbiota affects inflammation and immunity not only locally at the mucosal level but also systemically, which contributes to initiation, progression, metastasis and even treatment of cancer. In human stomach, the gastric mucosal microbiota has recently been investigated by high-throughput sequencing techniques, which showed that the gastric microbiota is more complex than previously appreciated. Considerable progress has been made toward understanding the altered composition and diversity of the gastric microbiota in gastric diseases (50–58), which has provided evidences that bacteria, including members of Proteobacteria, Firmicutes, Actinobacteria, and Fusobacteria phyla, can be regularly detected in gastric biopsies with gastric microbial dysbiosis associated with GC. Of course, the inter-personal variations among the stomach microbiota could not be ignored. The GC are multifactorial pathologies involving changes in the gastric microbiota, which can affect inflammatory processes of the stomach TME that contribute to cancer and its therapy. In human, defining inflammation and the presence of inflammatory cells within or surrounding the tumor is a critical aspect of modern pathology. Given the lack of understanding of the impact of inflammation on the progression of GC, it would be premature to design clinical therapeutic strategies that target inflammation as an approach to treating established cancers at the present time. However, numerous experimental studies suggest that changed immune response such as inflammatory cells and their associated mediators that regulated by gut microbiota form a microenvironment favoring the development of colorectal cancer, presumably by enhancing DNA damage in epithelial cells (59–61). Recent advance has indicated that cancer immunotherapy targeting these altered immune profiles has emerged as a powerful and promising clinical approach for treatment of cancer. The intimate interactions between host microbiota and immunological responses in TME may shed light on novel anticancer therapeutic strategies by manipulating human microbiota.
Traditionally, many previous studies have always considered the whole stomach as one habitat (50–58). However, the stomach microhabitats are not uniform; rather, they vary considerably across sites within the stomach, at the same site over time, and with health status. Different stomach microhabitats might determine different gastric microbiota and different gastric mucosal immunity, which formed quite distinct TME. In our present study, we investigated the connection between gastric microbiota and immunological responses especially immune escape in specific gastric microhabitats for the first time. Our present study found that higher relative abundance of the gram-negative phylum of Fusobacteria was associated with TME. Previous study has shown that Fusobacterium nucleatum (belonged to Fusobacteria phylum) can express the Fap2 protein that is able to interact directly with TIGIT (T-cell immunoglobulin and ITIM domain), an immune checkpoint marker that can be expressed on T cells in the TME, thus influencing patients' prognoses (62). In fact, it was more difficult to obtain healthy stomach tissues as controls; therefore, we screened confirmed GC tumoral, and peritumoral tissue, and neighboring normal tissue from the same GC patient to investigate discrepancies of gastric microbiota and its mucosal immunity in the TME, which could effectively screen key players for GC development.
From the three stomach microhabitats, the two major immunosuppressive cells, BDCA2+pDCs and Foxp3+Tregs, are significantly higher in peritumoral and tumoral tissues than those in normal ones. DCs are the most potent professional antigen-presenting cells of the immune system, either boost the immune system (enhancing immunity) or dampen it (leading to tolerance), depending on their maturation status (63). This dual effect explains the dual outcomes of cancer progression. Numerous experimental and clinical evidence shows that one DC subtype, pDCs, possess immunosuppressive and tolerogenic property and, thus, promote tumor growth and progression, which has been found to infiltrate in various cancer including GC (29, 64). Consistent with our previous study, we also found that pDCs preferentially accumulated in peritumoral tissue rather than that in tumoral ones (p < 0.05). This distribution bias has also been reported in cervical neoplastic lesions, in that pDCs were mainly detected in the stroma underlying the tumor rather than within the cancerous epithelium (65). BDCA2 is a novel type II C-type lectin presumably involved in ligand internalization, processing and presentation, as well as in inhibition of α/β-interferon (IFN-α/β) synthesis in pDCs (66). Tel et al. has demonstrated that immature pDCs can be readily detected by their expression of BDCA2, acting as a hallmark of human pDCs, which is down-regulated when pDCs mature (67), despite the latest finding conducted by Vescovi et al. has shown that BDCA2 staining remains detectable in toll-like receptor 7 (TLR7)-activated pDCs (68). pDCs show quite different functions in TME, depending on its immature or mature state. Mature pDCs acquire dendritic morphology, upregulate MHC and T cell costimulatory molecules, and produce high amounts of IFN-α (69), which can affect tumor cell proliferation and tumor metastasis (70), while immature pDCs cannot demonstrate the similar functions. With higher level of BDCA2 expressed in peritumoral and tumoral tissues, we speculated that the majority of pDCs in these tissues might belong to immature state. Similar to mechanisms previously shown in tumor cells (71–73), recent study conducted by Basit et al. showed that toll like receptor (TLR)-stimulation changed expression of genes regulating oxidative phosphorylation (OXPHOS) and glutamine metabolism in pDCs, while inhibition of glutaminolysis and OXPHOS prevented pDCs activation (74). Previous studies have also shown that immature pDCs recruited to the tumor site mediated by the interaction of CXC chemokine receptor 4 (CXCR4), resulting in promotion of pDCs-mediated generation of CD4+CD25+Foxp3+Treg cells, which leads to anergy and immune suppression, favoring the immune escape of tumor cells (75, 76). Hence, our current study has found a positive correlation between BDCA2+pDCs and Foxp3+Tregs in stomach tissues, which is consistent with the findings performed by Geva-Zatorsky and colleagues (8). In addition, several studies has found that the prognosis of different types of tumors is inversely related to the number of tumor-infiltrating pDCs (25, 26).
Tregs, one kind of tumor-infiltrating lymphocyte, played a crucial role in maintaining immunological self-tolerance when the immune system eradicated tumor cells. Previous studies have found that high density of tumor-infiltrating Tregs was accumulated in various human carcinomas (77–81). The function of these Treg cells has been described to suppress proliferation, induction/activation of CD8+ and CD4+ T cells in a COX-2-dependent manner, which is involved in establishing immunological tolerance in cancer immunosuppression (82). Treg-mediated immunosuppression has been considered as one of the crucial immune evasion mechanisms in tumor whereby they are able to overcome the anti-tumor activity of CD8+ cytotoxic T cell, DCs and natural killer cell (83). Different Treg subsets can produce different inhibitory cytokines and induce different lymphocytes, endowing Tregs with different functions. Increasing evidence shows that the transcription factor Foxp3 expression plays a key role in Treg-mediated dominant suppression and that Foxp3 expression significantly contributes to invasive breast cancer by tipping the balance toward a more immunosuppressive microenvironment (84–86). Yuan et al. has found that elevated Foxp3 expression in tumor-infiltrating Treg cells was associated with the TNM stage in GC patients (82). Previous study has also found that intratumoral Foxp3+Tregs/CD8+T ratio was an independent predictor for the prognosis of GC, with higher Foxp3+Tregs/CD8+T ratio representing worse overall survival (87). Our present study found that higher levels of Foxp3+Tregs were observed in peritumoral and tumoral tissues than that in normal tissue. Different from the alterations of BDCA2+pDCs, there was no significant differences of Foxp3+Tregs between peritumoral and tumoral tissues, which indicated that peritumoral and tumoral tissues showed similar TME that could not effectively prevent the invasion of tumor cells.
Both higher BDCA2+pDCs and Foxp3+Tregs in peritumoral and tumoral tissues demonstrated the disturbance of gastric mucosal immunity homeostasis. Although commensal bacteria were crucial in maintaining immune homeostasis of the intestine, the roles of gastric microbiota on gastric mucosal immunity regulation were still unclear. Our present study found that the gastric microbiota was not uniform among the different microhabitats in the gastric TME, with decreased diversity in peritumoral tissue and decreased species richness in peritumoral and tumoral tissues, while the composition of the gastric microbiota changed significantly in tumoral tissues. Geva-Zatorsky et al. has demonstrated that microbial diversity in the gut ensures robustness of the microbiota's ability to generate a consistent immunomodulatory impact, serving as a highly important epigenetic system (8). Previous studies have found that the increased abundance of HP in the tumoral sites is classically believed to contribute to GC tumorigenesis, and mass eradication of HP significantly decreases the risk of developing cancer in infected individuals without pre-malignant lesions (88–90), reinforcing the theory that HP influences early stages in gastric carcinogenesis. However, our present study only found higher relative abundance of HP in peritumoral tissue than that in normal and tumoral tissues, but no correlations between HP and gastric immunosuppression. This might be associated with its "hit and run" mechanism for GC development, which HP is no longer present in the intratumoral microhabitat at the time GC is identified (91). The changed hypochlorhydric mucosal environment in tumoral tissue was not suitable for HP colonization. Although HP was not correlated with immunosuppressive cells, its colonization could also affect the overall structure and composition of the gastric microbiota, which might affect the antitumor immunity and participate in GC development indirectly. Our study found that many non-dominant gastric bacteria were significantly correlated with the two major immunosuppressive cells. Most of these bacteria belonged to the phyla Proteobacteria and Firmicutes, which modulated gastric mucosal immunity alone or in together with its bacterial metabolites. Fujiwara et al. described a reduction in pDCs in mice with a restricted microbiota distinct from that typical of specific pathogen-free mice (92), whereas Dasgupta et al. revealed induction of pDCs in mesenteric lymph nodes by B. fragilis during ongoing colitis (93). Recently, Geva-Zatorsky et al. has found that the frequency of pDCs in colon and small intestine is influenced by gut microbiota but with great variable in germ-free mice monocolonized with one commensal bacterial strain. They found that the transcripts (from the colons of monocolonized mice) most correlated with pDCs frequency were enriched in lipid or protein digestion and metabolic pathways, an observation suggesting a connection between pDCs and the metabolic and nutrient uptake functions of the gut (8). Previous study found that Stenotrophomonas was associated with a wide spectrum of diseases and substantial morbidity/mortality in immunosuppressed patients with cancer (94). Stenotrophomonas showed immunostimulatory properties, inducing TNF-α expression and contributing significantly to inflammation, which might participate in TME modulation in the stomach. Our present study firstly demonstrated that Stenotrophomonas was positively correlated with BDCA2+pDCs. Comamonas was a cellulolytic microbe, which could impact the host metabolism in cancer patients and are associated with inflammation (95). Recently, Garcia-Gonzalez et al. has demonstrated that Comamonas could decrease 5-fluoro-2'-deoxyuridine efficacy, but increase the efficacy of the topoisomerase I inhibitor camptothecin (96). In our present study, we found that Comamonas was negatively correlated with BDCA2+pDCs, which might play a role in antitumor immunity. Our previous study observed that Selenomonas was enriched in patients with colorectal cancer and almost absent from healthy individuals (35). In GC patients, we also found higher relative abundance of Selenomonas in peritumoral and tumoral microhabitats, which exerted local effects on the immune system and was correlated with Foxp3+Tregs positively. In addition, a new microorganism often isolated from soil, Gaiella, also showed immunostimulatory properties and was negatively correlated with gastric Foxp3+Tregs through an unknown mechanism. Our present study found that the functional content of the gastric microbiota changed significantly in tumoral tissue, especially in metabolism-related pathways. Overall, the impaired gastric mucosal immunity might be the results of the interplay between the gastric microbiota and the stomach, which lead to tumor escape from immune surveillance.
In summary, we firstly demonstrated the relationship between gastric mucosal microbiota in different microhabitats and its corresponding gastric mucosal immunity in GC patients. The recruitment of two major immunosuppressive cells, BDCA2+pDCs and Foxp3+Tregs, represented the disturbance of gastric mucosal immunity homeostasis in the gastric TME that contributed to the GC development. The structure, composition and function of the gastric mucosal microbiota changed significantly among different microhabitats. Interestingly, we found that the gastric microbiota especially those non-abundant genera was correlated with BDCA2+pDCs and Foxp3+Tregs significantly. Our findings implied that the gastric microbiota might participate in modulating tumor-immune microenvironment actively, which indicated that gastric microbiota might be a key orchestrator of cancer therapy. Our present study will provide new insights on personalized GC diagnosis and treatment by modulating TME by gastric microbiota.
The present research was approved by the Ethics Committee of the First Affiliated Hospital, School of Medicine, Zhejiang University (Zhejiang, China). Informed written consent was obtained from each of the patients before enrollment.
ZL and XsL conceived and designed the experiments. ZL, XsL, LS, XL, YC, YM, FJ, and CY performed the experiments. XsL, LS, and ZL analyzed the data. ZL, LS, and XsL wrote the paper and edited the manuscript. The final manuscript was read and approved by all authors.
The authors thank all of the participants who recruited patients in this study. This present work was funded by the grants of the National Natural Science Foundation of China (81771724, 31700800, 81790633, 81273254, 31870839), National S&T Major Project of China (2018YFC2000501), Science and Technology Planning Project of Zhejiang Province (2015C33174), Zhejiang province key science and technology innovation team (2013TD13), Major Project of Science and Technology Department of Zhejiang province (2014C03040-1), and Project of Zhejiang Provincial Education Department (Y201326985).
24. Hartmann E, Wollenberg B, Rothenfusser S, Wagner M, Wellisch D, Mack B, et al. Identification and functional analysis of tumor-infiltrating plasmacytoid dendritic cells in head and neck cancer. Cancer Res. (2003) 63:6478–87.
70. Mccarty MF, Bielenberg D, Donawho C, Bucana CD, Fidler IJ. Evidence for the causal role of endogenous interferon-alpha/beta in the regulation of angiogenesis, tumorigenicity, and metastasis of cutaneous neoplasms. Clin Exp Metastasis. (2002) 19:609–15.
76. Moseman EA, Liang X, Dawson AJ, Panoskaltsis-Mortari A, Krieg AM, Liu YJ, et al. Human plasmacytoid dendritic cells activated by CpG oligodeoxynucleotides induce the generation of CD4+CD25+ regulatory T cells. J Immunol. (2004) 173:4433–42.
92. Fujiwara D, Wei B, Presley LL, Brewer S, Mcpherson M, Lewinski MA, et al. Systemic control of plasmacytoid dendritic cells by CD8+ T cells and commensal microbiota. J Immunol. (2008) 180:5843–52.
Copyright © 2019 Ling, Shao, Liu, Cheng, Yan, Mei, Ji and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.


RHHS members of the National Honor Society shared a little bit of their Saturday helping the Easter Bunny Hide treats for the annual Easter Egg Hunt at the city park.
On Thursday, March 22, the RHHS track teams opened the season at the Butler Invite. The following athletes placed in the top eight for their events and scored points for the team.
Games scheduled for tonight against Lakeland have been cancelled.
Mrs. Wheatley's French II & III and Spanish II & III send their thanks to Mrs. Heather Diehl and Farmers Insurance. Mrs. Wheatley received "Teacher of the Month" for January and the donation that came with it was used to purchase groceries for the students to try out a few recipes. Keep your eyes out for their "cooking shows". They are in production now.
The baseball game with Lamar has been moved from 4-3-18 to 4-18-18.


text:
Saiguède es una comuna (municipio) de Francia, en la región de Mediodía-Pirineos, departamento de Alto Garona, en el distrito de Muret y cantón de Saint-Lys.

Su población en el censo de 2006 era de 689 habitantes.

Está integrada en la Communauté de communes Coteaux du Saves et de l'Aussonnelle.

Demografía

Referencias

Enlaces externos

 INSEE
 Elecciones municipales de 2008

Saiguede

redpajama_set_name: RedPajamaWikipedia


Property Description: This is an intimate, unique beachfront residential apartment on Rawai Beach in southern Phuket due for completion in Q3 2015.
Built by an English developer to a high specification and European standards the apartments enjoy features such as cavity walls for sound-proofing, an excellent feature in apartment living.
There are only 7 apartments in total, with large 160sqm 2-bedroom units still available.
At present the interiors are incomplete since the Developer would prefer to offer potential buyers the opportunity to choose their own tiles and kitchen fittings.Samples of those chosen are per the photos.
Each apartment enjoys not only spacious internal accommodation with a central open-plan living/dining/kitchen area and two good-sized en-suite bedrooms to either side for maximum privacy, but also a very large covered terrace. This is equipped with a fitted wet-bar and outdoor kitchen for entertaining.
This area can be used whatever the weather and extends the living space considerably.
From here and from the comfort of each room within the interior owners and their guests can enjoy the fantastic sea views across the sparkling waters just 50 metres away, and the outlying islands.
Contact today for full details as only a limited number of units remain.
Neighborhood Facts: Rawai Beach, Phuket is a 'best kept secret' boasting untouched natural beaches, sand and palm trees, a perfect holiday location close to all amenities.
The complex is an easy walk to the wealth of restaurants offering both Thai and International cuisine, bars, shops, banks, cafes etc.
The small sandy beach of Laem Ka is less than a 10-minute walk away and the fresh seafood market is within walking distance offering the freshest catch of the day which can be taken home for a BBQ or cooked to perfection at the restaurants opposite.
Chalong, with its supermarkets such as Tesco, Makro and Villa Market is around a 10-minute drive away, whilst Phuket Town offering cinemas, shopping malls, boutique restaurants, bars and entertainment can reached within 20 minutes.
Phuket airport is approximately 55-minutes away along the central highway which bisects the Island.


Two ex-gfs are just hanging out on a bed wondering what to do with their day. Suddenly Johnny, Peppers step brother, walks by her room with just a towel on. Dani can't stop staring at Johnny's meat. Being naughties, these two nice young lookings jump on that meat as quickly as possible and have a numerous amount of orgasms in the process.


Our "guest" at Thursday of Borrowed words is Leigh Richmond ( 1772- 1827); He wrote 15 marriage admonitions for his daughters... and us.
When your sister Mary left her paternal roof, I gave her a paper of admonitions, which I requested her to read often. I do the same for you, in the form of a friendly string of maxims, to regulate your conduct in your new and very responsible situation.
1. Aim at keeping a devoted heart for God in the least and most common transactions of every hour—as well as in those events which may seem to call the loudest for manifestations of Christian prudence and principle.
2. Pray regularly and frequently, not seldom and occasionally—for grace to live by.
3. Remember the Christian principles and examples of your father's house, and everywhere endeavor to preserve its character, by consistency in conduct, conversation, and temper. Keep in constant recollection—the wise, prudent, and conscientious example of your dear mother.
4. Form no hasty friendship; and none whatever, but such as may promote seriousness of heart, tongue and life.
5. Beware of cheerfulness degenerating into levity. Let no natural vivacity of temper, no occasionally indulged sallies of humor and jocularity—throw a shadow over the exercise of solid principle. Little foolish things give a color to character, and are more easily imitated, than serious and good sentiments.
6. Guard against hasty judgments of character, and above all against uttering hasty opinions, and making remarks to the disparagement of others. Particularly avoid making the errors, failings, faults, or follies of others—the subject of rash and unguarded remarks. Be known for charity, forbearance, and kindness. Be slow to judge—rather than swift to speak.
7. Wherever you are, in the first place, remember that God's eye is upon you; and then imagine also that your husband and father are present. It may be a fanciful—but it is a profitable supposition.
8. Keep Christ's golden rule,"Do unto to others—as you would have them do unto you" in perpetual remembrance. It is the panacea for most of the social evils of life.
9. Be conscientious towards all;friendly with few; confidential with fewer still; strictly intimate with fewest of all.
10. When you think of your father, bear with his infirmities and pardon his faults—but remember his principles and instructions, so far as they have been agreeable to the Word of God.
11. Do not be content with anything short of deep, sincere, diligent, and decided piety.
12. If you and your husband happen to differ in opinion or feeling on any point—remember whom you have promised to love, honor, and obey—and this will settle all things.
13. Of your husband's warm affections towards you, I entertain no doubt—strive to preserve them by daily elevation of character; and not so much by fondness—as by prudence and dignity. May you both learn to raise a home of marital happiness—by mutual wisdom and love.
14. Observe great simplicity and plainness in dress. You should be a pattern to others in this respect. There is a just complaint made of many females who profess to be pious—that they are far too showy and mirthful in their outward apparel. Remember the apostle Peter's injunction, "Your beauty should not come from outward adornment, such as braided hair and the wearing of gold jewelry and fine clothes. Instead, it should be that of your inner self, the unfading beauty of a gentle and quiet spirit, which is of great worth in God's sight."
15. Christ has been made known to you fully and freely; let Christ be your all in all, both now and forever.
Many of these advices are forgotten, many sound "out-dated", even for some women who profess to be Christians; but let's be sincere, Christian women nowadays desperately need to take some of these godly advices.
Which other would you include?


text:
Dr. M.P. Narayanan
Dr. M.P. Narayanan is an eminent administrator and technocrat with a long and successful innings behind him holding important official positions. Dr. Narayanan currently apart from running a prominent scientific NGO also contributes his expertise to various high power think tanks/committees. His strong area of interest is planning for sustainable development. In this quest Dr. Narayanan has been active in projects in areas such as disaster management, environmental rehabilitation, freedom of information, IT for the common man etc. A Mining Engineer by profession, committed to Public Sector Management ethos and a proponent of Innovative Man-Management approach of functioning. Has served the Public Sector and sustainable development over a period of 33 years. After serving the Public Sector in the Senior most capacities for about 33 years, Dr. Narayanan has started his initiatives in developing public awareness in the field of Information Technology. He started involving himself vigorously with 2 NGOs viz. Centre for Science, Development and Media Studies (CSDMS) and GIS Development. Apart from his involvement in Information Technology fields, Dr. Narayanan has been very actively involved in the activities of World Mining Congress. He was one among the leading personalities who took part in bringing up a very grand success of World Mining Congress, 2003 held in New Delhi, India. He was also one among the active persons who took part and bringing up successfully World Mining Congresses in Iran, Turkey and Poland subsequently. M.P. Narayanan believes in creativity in work, practices inter-disciplinary approach in decision making and is rooted in Indian tradition and culture. Always in Quest for Excellence.
Water Balancing Plans through data-driven Solutions
News Update/Press Release August 23, 2021, New Delhi: Safe Water Network, the internationally renowned NGO, and USAID presented their insights on 'Making City Water Positive through City Water Balance Plans'
Role of Csr in Nation Building
Recently, India became the world's fifth largest economy by overtaking the United Kingdom. Now, the United States, China, Japan, and Germany are the only nations with economies larger than India's
Sport of Life initiative to support injured players in need
National, 4th March 2022: Healthium MedTech, a global MedTech company, today inaugurated the 'Sport of Life' initiative, with Abhinav Bindra Foundation Trust (ABFT). ABFT aims to support injured players in
Test press release
Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Maecenas porttitor congue massa. Fusce posuere, magna sed pulvinar ultricies, purus lectus malesuada libero, sit amet commodo magna eros quis urna. Nunc
Maruti Suzuki partners Government of Haryana to set up State's first Japan-India Institute for Manufacturing (JIM)
PRESS RELEASE New Delhi, January 28, 2019: Maruti Suzuki India Limited (MSIL) has signed a Memorandum of Agreement (MoA) with Government of Haryana to set up Japan-India Institute for Manufacturing (JIM),


This is a really fun, hands on Sunday school lessons that is a Jesus and Nicodemus Lesson that talks about measuring God's love using the text John 3:16. This is a wonderful lesson for the month of February and Valentines Day.
This weeks Jesus and Nicodemus Lesson covered John 3:1-21 and measuring God's love and we did lots of hands on kids activities and crafts themed around Measuring God's Love and John 3:16. This lesson is perfect for 1st grade, 2nd grade, 3rd grade, 4th grade, or 5th grade kids especially in February around Valentines Day.
We started about by giving each of the kids a ruler, a pen, and this card (printable included below). On it were several things for them to measure. We explained that these were ways for the kids to measure distance, time, height etc. in preparation for our lesson today when we were going to measure God's love for us.
We had several stations with stop watches to measure time, tape measures to measure height, measuring cups to measure rice, and more.
When we came back together as a group, we talked about how we can measure things, but can't measure God's love; it is infinite! And it will NEVER run out!
This is such a cute, simple craft for kids! Just print the John 3:16 Valentine Heart below on cardstock. Then, the kids will use a hole punch to make holes around the heart. Next they will color in the hearts in the center that spell "valentine". Finally, they will use about 2 feet of red yarn to sew the heart. around the outside.
The kids really enjoyed this craft and it was perfect to make right around Valentines Day. We encouraged the kids to think if there was someone they knew they could give the heart to, to share God' love with others!
You are welcome to use this at home, at church, homeschool coops, etc.
I don't see the Jesus and Nicodemus lesson on TPT. I am the Director of Children's Ministry at our church. I would like to offer the craft and measuring activity to our volunteers. Is there something I am missing or a way to purchase for use in our Sunday School classes? Thanks!
you are welcome to use any of our bible crafts FREE at churches as long as they are unaltered.
I can not find this lesson on your TPT Store either. Is there another way to access this lesson?
I don't see a link to view/download the Jesus/Nicodemus lesson…?
These are all the hands on activities / crafts we used for the lesson to supplement the Sunday School curriculum we were given to make it more engaging. You could grab a video from you tube, read teh story from the Bible, do a skit or puppet show of the story from the Bible in John 3:1-21.


Floral design attached to the car will produce shades of cool, beautiful and gorgeous. In addition, ..
Whatever form the flower designs will be used as car sticker, it would produce a highly artistic wor..
According to the designer, the image above is made because his heart was hit romance. He wants to ha..


Moo! Grill an Argentinian coffee and wine bar serving Lomitos and empanadas. The concept is from Cordoba, where the Lomitos (steak sandwich) is very popular. A year and a couple months in the location, serving excellent Argentinian steaks, beer, and of course wine, definitely a unique place.
I bump into this place after reading a couple reviews. Arrived with a couple friends and immediately I felt like home, the service is perfect, obviously it helps speaking in Spanish but Moo! Makes sure everyone is treated like part of the family, is more like you're in a friend's house having drinks and a bbq.
I had a late lunch that day, and wasn't going to eat too much until I saw the menu, I wanted to try everything as usual! And the prices are really accessible. So I went mental, basically I order everything that caught my eye and attention. Empanadas checked, one with beef the other humitas checked. Then I went for a Beef Lomito checked, and a Choripan checked. Two huge and tasty sandwiches in toasted ciabatta with salad, sauces, and other trimmings, in the case of the Lomito the ham, the melted cheese, and the egg was the perfect company for the tasty Argentinean beef. While the Choripan with roasted peppers completed the scene.
The food was amazing period. To refresh the evening Quilmes, and some Argentinean wines gave the extra pleasure for the night. If you want to try something more substantial, you can go for any of their steaks, Bife de Lomo, Bife de Chorizo, the latter my favourite.
I will be returning regularly to Moo! To be honest is a no brainer, good food, nice beers, excellent wines, centrally located, friendly service, and reasonable prices. Too bad the owner is not a Boca fan, but hey nobody is perfect. Che boludo que esperas para ir!


lowqui.com ~ Prepossessing Pokemon Lucario Coloring Pages In Beatiful Pinterest You can quickly transform that space into something usable and comfortable. We take a closer look at it as well as some alternatives. Prepossessing Pokemon Lucario Coloring Pages In Beatiful Pinterest is one way to turn a dingy unfinished room into additional space that's attractive and comfortable. It does this by applying their expertise in building science and system design to the .


text:
Q: (optimization) Do i change "SpriteRenderer.Sprite" to the same texture every frame, or condition before? I know it's a simple question but i really don't know the answer, what is the the most optimize way to do ?
first (condition) :
        if (sprRenderer.Sprite != Sprites[Frame])
        {
            SprRenderer.sprite = Sprites[Frame];
            Debug.Log("draw");
        }

second (without condition) :
            SprRenderer.sprite = Sprites[Frame];
            Debug.Log("draw");

if you want more details that it's the update code (on start 'timer' = 1, 'AnimParameter' it's an event that loop animation, 'NameAnimation' it's this animation) :
    void Update() {

    Timer += Time.deltaTime;

    if (Timer >= 1f / FramePerSec)
    {
        if (Frame < Sprites.Length - 1)
        {
            Frame++;
        }
        else
        {
            if (AnimParameter.CurrentAnimation != NameAnimation)
            {
                enabled = false;
            }

            Frame = 0;
        }

        SprRenderer.sprite = Sprites[Frame];
        Debug.Log("draw");

        Timer = 0;
    }
}


If you've been following the news and reading the details religiously, you will know that pretty much everything that you love is now dangerous to health. It seems like the ingredients that go into the best dishes in the world are going to kill us all.
Last year, they took Maggi off the shelves because of the high lead content. While that's a good reason to take something off the shelf, why hadn't anyone noticed it before? This year, everything from bread to hot coffee seems to be a troublemaker and the list of things we can eat or drink without double checking Google seems to be getting shorter and shorter.
These foods have been around for years and people have eaten them in various different shapes and sizes and forms, but it's now that everything is setting our bodies off. Is it the ingredients that are leading us to death or have our bodies changed so much that we don't know how to handle it?
The list just keeps on growing, because clearly every single day there's something going wrong with the food in the world. Sure, the population is increasing and so is everything that goes with it. With the increase in population and the increase in demand for products, it might be a problem to be able to produce the same amount all the time. So there's a chance that a lot of ingredients are being replaced with unhealthy substitutes just to make up for what's missing.
Putting aside the obvious factors why junk food is unhealthy for us, there are so many other things that are considered unhealthy and when you're done with this list, you're going to feel like you're missing out on so much in life.
What is considered unsafe these days?
– Bread: A study found that there were cancer-causing chemicals found in bread in India. Whether all bread is dangerous or just a certain kind is still unknown, but if it's in one kind of bread, what are the chances of it not being in the others?
– Alcohol: A brand new analysis found that alcohol is the primary cause of seven different kinds of cancer. The more you drink, the higher the risk. So all the studies you've read about a glass of wine being good for you are now being debunked, because seven kinds of cancer is no joke.
– Hot Drinks: This might just be the most bizarre thing we've read all year. In June of this year, it was reported that hot drinks above 65 degrees Celsius could cause people to develop cancer of their esophagus, the eighth most common form of cancer worldwide. So if you're a fan of the hot stuff, take it down a notch.
– Meats: A WHO report revealed that eating processed meat can lead to bowel cancer, while red meats are probably carcinogenic. They even categorized processed meats like hot dog, sausage and bacon in the same group with tobacco, asbestos and diesel fumes, which are proven carcinogenic agents.
– Absinthe: You probably didn't know this and that's okay, because nobody did, but the hallucinogen part of the drink is made from a toxic chemical named thujone, which is not good at all. Besides having hallucinations, it could do a lot more damage.
– Instant Noodles: In some situations, people still believe that Maggi and Ramen and other instant noodle brands are bad for health. While they're being tested and retested constantly, the fear that they are not safe continues to haunt certain people.
Besides avoiding these foods, there isn't a lot that you can do to keep yourself healthy. Everything you assume is okay to eat, might suddenly be proved wrong. It's just a case of balancing out your diet and eating carefully, because anything else might be a little too difficult to do really.
Hi Surya, you can find all our current recipes here: https://www.hungryforever.com/category/recipes/ If there are any that you can't find, email us and let us know. Or contact us via Facebook.


text:
Donation to MPD leads to a purchase of ballistic shields for officers
Posted: Jan 24, 2023 12:02 PM CST
Photo credit: iStock images, user: zabelin
MILWAUKEE (CBS 58) -- The Milwaukee Police Department has announced the purchase of special tactical ballistic shields as a direct response to multiple MPD officers being injured by gunfire, or being shot at, while on duty.
A press release from MPD on Tuesday, Jan. 24 notes that the purchase aims to better protect officers in life-threatening situations, particularly given the proliferation of guns and other firearms in Milwaukee.
A donation by Minnesota-based Johnson Brothers is what made the purchase possible, according to MPD. The company has done business in Milwaukee since 1975.
Bill Anderson, President of Johnson Brothers of Wisconsin said in the press release that the donation was rooted in an appreciation for the work of MPD officers. "They put their lives on the line every day, and this donation is simply our way of saying 'thank you,' and a token of our appreciation for the critical work they do every day in keeping our community safe."
Chief Jeffrey Norman expressed gratitude for the donation, noting, "We can't thank them enough."
Mark McClain, President of the Milwaukee Police Foundation believes that the shields will allow MPD to have the necessary tools and resources to be safer while helping build community trust.