text:
Q: How do we get/define filters in convolutional neural networks? How do I obtain filters from convolutional neural network(CNN)? My idea is something like this: do random images of the input images (28x28) and get random patches (8x8). Then use autoencoders to learn the common features of the patches (features = hidden units; approximately 100, for example). Then apply features filters to the input images and do convolution. Am I correct?
I am confused because sometime the literature state only using like, e.g. 8, filters, but in my case I have 100.

A: Convolutional filters are usually trained along with the rest of the network weights. The Tensorflow tutorial is a good place to start understanding convolutional neural networks in practice. https://www.tensorflow.org/versions/r1.0/get_started/mnist/beginners

redpajama_set_name: RedPajamaStackExchange


We began our day with a ramble through the Mendocino headlands, followed by blueberry Danish and fresh eggs from the chicken coop (poor chickies). Seriously, we really want to move to Mendocino.
It's beautiful. This was the view from the garden of the Bed & Breakfast we stayed at (though our "farm retreat" was two blocks up from the main building).
There's a music festival starting tomorrow, and the tents are already set up.
The architecture is simple and lovely. Everything is peaceful and you can walk on the beach undisturbed by other people.
We clambered down some rocks and found tidepools.
After strolling around town some more, we headed north… and before long found ourselves in densely forested redwoods.
I don't know why, but these woods seem to be a haven for all kinds of kitsch. This store was representative.
The forests are full of God, though, apparently.
Still, it was a beautiful drive, though naturally it felt worlds apart from the coastal highways.
Luckily we're back to the coast now, and tomorrow will head into Oregon! We're spending the night in Crescent City at a perfectly nice motel, the Lighthouse Inn, getting our rest in preparation for another long drive tomorrow.
I don't know how I am ever going to get through all these photos after I get back.


If you're increasing your online business or transferring to North Carolina, or making modifications to your small business, verify this website for the enterprise registration, licensing, tax and employee implications. Go to the U.S. Small Enterprise Administration web site to discover a tutorial on how to create a business plan.
Right here I will assist you figure out the best way to start up your enterprise so you can start making money utilizing your Cricut or Silhouette machine. The Secretary of State publishes the Submitting Guide, which presents pointers for registering business entities.
There are a number of crucial steps involved in starting a enterprise, including writing a marketing strategy, securing financing, researching and choosing a location, registering your small business, complying with tax necessities, and filing native and state licenses and permits.


text:
Gonzalez sworn in as Brooklyn's 33rd DA
By Nelson A. King
Brooklyn District Attorney Eric Gonzalez takes his oath of office.
Office of Brooklyn DA
Eric Gonzalez was inaugurated as the 33rd District Attorney of Brooklyn during a rousing ceremony at the Weylin in Williamsburg on Sunday, during which he outlined his vision of keeping Brooklyn safe and strengthening communities' trust in the criminal justice system by ensuring fairness and equal justice for all.
The event was attended by elected officials, judges, clergy members and community leaders.
Mayor Bill de Blasio opened the ceremony and Attorney General Eric Schneiderman, Public Advocate Tish James and Council Speaker Corey Johnson, among others, delivered remarks.
The Oath of Office was conducted by New York Chief Judge Janet DiFiore and salsa legend Willie Colon provided musical entertainment.
"I am so thrilled and honored to be standing here today. Growing up, my dream was to serve as an assistant district attorney in the Brooklyn DA's office. That's it. Never in my wildest dreams did I imagine that one day I would be sworn in as the District Attorney. I am humbled and honored beyond my ability to express," said Gonzalez in his address, thanking all present, including his family, for his success.
"I also want to acknowledge, and to remember, one towering figure who isn't with us today. And that is my friend and mentor, the late Great Ken Thompson [his deceased predecessor]," Gonzalez added. "We all imagined that today would be Ken's day, his second inauguration, and that we would be looking back on his accomplishments, and looking forward to the future. So, this is a bittersweet moment for me, and for many of us.
"I know that Ken would be so proud of where the Office stands today, of how the staff held together to carry on his legacy of reform," he continued. "We stand tall today on Ken's shoulders, and we look forward to a bright future because of the groundwork he laid in the far too short time he held the office of Brooklyn District Attorney."
Gonzalez said he was proud to work with Thompson on the reforms for which the Brooklyn District Attorney's office "continues to be known around the country."
He said the Conviction Review Unit, with 24 wrongful convictions vacated thus far, is the national model.
He also said the Begin Again program, in which nearly 150,000 warrants were vacated in a single day in Brooklyn, has contributed significantly to reducing the enormous backlog of warrants that have prevented thousands of New Yorkers from moving forward with their lives.
Gonzalez said the Young Adult Court is one of the most innovative in the country, helping young people get the services they need to avoid further criminal justice involvement.
"We have pursued all these reforms while keeping Brooklyn safe," he said. "We closed out 2017 as the safest year in my lifetime, and very possibly in yours: shootings and homicides hit record lows. Assaults, robberies, larcenies were all down."
But even with those accomplishments, the DA said there is more work to be done, announcing the Brooklyn DA's Justice 2020 Initiative, aimed at "keeping Brooklyn safe and strengthening communities' trust in our criminal justice system by ensuring fairness and equal justice for all."
He said he intends to make significant progress toward this vision by the end of 2020 by promoting a justice system predicated on fairness, equity, compassion and fiscal responsibility; doubling down on "our obligation, as prosecutors, to do justice, not just seek convictions;" working "toward outcomes that restore and heal victims and communities, and we will work to reduce racial disparities in our system;" and continuing to "identify and focus on those who do the most harm."
"In Brooklyn, what we won't do, however, is be passive in the face of cruel and misguided policies handed down from Washington, D.C., especially on immigration," Gonzalez affirmed.
"Our recently-formed Immigration Unit is being adopted by prosecutors across the country and rightly so — it helps our friends and neighbors in immigrant communities avoid unjust deportation and sends a message to the rest of the country that when it comes to our immigrant communities, doing what's right and fair doesn't conflict with our commitment to public safety," he added. "Rather, it enhances it; it makes us safer."
Kings County courts celebrate Caribbean Heritage Month
Brooklyn acting DA working for immigrant communities' rights
New policy for immigrant defendants
Clarke: Oppose Trump's immigration policies

redpajama_set_name: RedPajamaCommonCrawl


We wanted to send a quick reminder to those of you with a fiscal year ending June 30th. First is a summary of your options for when you actually execute your year end. Second are a few links to documents that can assist you with finalizing your MS-61 Report and generating a database backup.
The Avitar Tax Collect system has a hard fiscal year end close, which means that you cannot process work in the current fiscal year until the last fiscal year has been closed. When you close the fiscal year, the system captures the ending balances (outstanding invoice amounts by warrant) for that fiscal year and creates the beginning balances for the next fiscal year. The beginning and ending balances are extremely important as the beginning balances of this year must match the ending balances of the previous year. If they do not, you must explain to the Department of Revenue (DRA) why they have changed. With this in mind, the system was designed not to allow changes in a closed fiscal year, hence the hard close.
Close on July 1st: You can choose to run all of your reports, balance for the year, and close the fiscal year on July 1st (or immediately thereafter such as, for example, the morning of July 1st). This option allows you to then post payments for July and is recommended for those towns with a 2017P01 due date of July 1st. However, be aware that once you close you will not be able to post payments with a payment date prior to July.
Leave your books open and close by July 10th: By law, you can leave your books open for ten days into the next fiscal year, in order to post any payments you receive for June. Keep in mind that you should only be processing payments during this time, not assigning credits or posting abatements. All other transactions should have been completed prior to year-end. Note, this option will not allow you to post July payments until after you have closed your fiscal year.
It is up to you on which option you prefer, but remember that if you balance and run your reports on June 30th and subsequently post a payment or process any transaction (assign a credit, process an abatement, delete a transaction) you will need to rerun your reports. Note that if you do any work after June 30th and want it to be reflected on your Monthly Balance Report you will need to call us in order to recapture your June report.
Lastly, we have also provided documents that review in more details instructions for reviewing your MS-61, as well as instructions for creating a backup of your data (File | Backup Database).
This entry was posted in Tax Collect Software and tagged Close Fiscal Year, MS-61 Report, Year End. Bookmark the permalink.


China River Stone Crushing Plant manufacturers - Select 2018 high quality River Stone Crushing Plant products in best price from certified Chinese Stone Crusher .
China High Capacity River Stone Crushing , China High Capacity River Stone Crushing Plant for Road Construction (300TPH), Find details about China Stone Crushing Plant, Crusher from High Capacity River Stone .
Aug 01, 2016· Baichy self-designed and manufactured rock stone crushing plant, all direction services installation, guide, debug, adding device and modify according to act.
china stone crushers plants - libertyeduorg As China stone crusher plant manufacturer, supplies stone crusher plant for sale In the stone crusher plants, jaw crusher is used as primary crusher machine .
china stone crusher, stone crusher manufacturers , rock jaw crusher, rock crusher, jaw crusher quarry crusher is widely used for crushing various materials like stone, granite, trap rock, coke, coal, manganese ore .
Stone Crusher Plant for We recommend you the impact stone crusher we have sold many secondary crushers in the world, such as India, China, USA, Chile .
Jaw Crusher is a kind of Stone crusher, , for Stone crusher plant; Primary jaw crusher is widely used , Industries Development Zone of Zhengzhou, China .


ROI Marketing is investing in improvements that will allow it to enhance not only its own operations, but also its customers' capabilities. The company's goals for 2016 include setting up a regular weekly training session for contractors, builders and engineers who use and install the products ROI provides to wholesale distributors.
The company is a manufacturer's representative for decorative kitchen and bath products, plumbing fixtures and heating and air conditioning products. ROI serves a territory that includes Virginia, Maryland, the District of Columbia, Delaware, Eastern Pennsylvania and southern New Jersey. Products represented by the company are used in residential, commercial and industrial applications.
Rich-Tomkins Company's sales staff members are like athletes: They receive coaching to achieve their highest possible level of performance. "What sets us apart is our professional sales organization – we have brought in third-party professional sales coaches who work with our staff," President Stew Chaffee says.
In the complicated, ever-evolving world of electronic technology, an OEM that is trying to build an electronic device sometimes has trouble locating a supplier for its components. Similarly, companies that manufacture electromechanical or electronic components have difficulty knowing what components are being sought by OEMs. Bringing the two together with product expertise and market knowledge are the manufacturer's representatives of O'Donnell Associates North.
"Our primary job is to help the manufacturer create demand in the marketplace for their products," President Mark Conley explains. "We do that primarily by calling on the engineering community to understand what our customers are building, and then offer products that match what they need for their product. We match up the manufacturer with the customer and are extremely involved throughout that process.
Based in Mississauga, Ontario, Canada, the company represents manufacturers in the electrical industry, including General Electric Co.'s Lighting Division, The Eden Corp. and Lennox International Inc. Northwinds Corp. started operations in the 1990s as a service provider for Affiliated Distributors.
The company followed that path for 11 years. "In 2007, we changed our format to become a traditional sales agency, and the rest is history," it says.
With fewer people pursing vocational careers, trades-oriented businesses such as MJM Associates Inc. are putting a premium on recruiting capable employees. "One of the biggest challenges we have are the lack of qualified people entering the industry," says Cam Marklowitz, president of sales and marketing for MJM Associates, a company that represents manufacturers in the plumbing, heating, ventilation, refrigeration and air conditioning industries.
A lack of knowledgeable, skilled workers could hinder MJM Associates' ability to grow and provide a high level of service for its wholesaler and contractor customers. This is why the company is assisting trade schools in recruiting high school students. MJM Associates employees travel to vocational schools to lead training classes and Marklowitz serves in an advisory board at a community trade school near the company's headquarters in Centennial, Colo.
When it's minus 10 F and a rest home loses heat, people depend on a contractor to get it back up and running right away. The contractor depends on Michel Sales Agency to provide the support to make that quick response possible.
Michel Sales Agency is a manufacturer's representative for the plumbing, heating, ventilation and air conditioning market. The company sees itself as an extension of the manufacturer, President Kelly Michel says, and takes pride in the products it sells and supports.
Founded by Roy Hollabaugh Sr. in 1945 in Portland, Ore., Hollabaugh Brothers & Associates (HBA) is today a fourth-generation family business. The company's aim is to be the top manufacturer's representative in the plumbing and HVAC wholesale industry in the Pacific Northwest through superior marketing, sales, service and product knowledge.
For more than 67 years, Dawson Co. has represented and distributed some of the biggest and best-known names in the plumbing and HVAC industries throughout Southern California. The reasons for the company's prolonged success in one of the nation's largest markets are rooted in its bedrock principles of exceptional customer service and strategic financial management, according to Executive Vice President Frank Dunn. As the company looks forward to the future, it's safe to say that those business principles will continue to be the foundation on which Dawson Co. builds its continuing success.


text:
Home Campus DU PG Admissions 2019: Here's All You Need To Know
DU PG Admissions 2019: Here's All You Need To Know
Manas Dixit
After three (or four) short-lived years in an undergraduate course, all the graduates have this one question in mind – What next?
The ones who have a job offer in hand can sit back and relax until their date of joining.
The ones preparing for various competitive exams, meanwhile, put their feet down on the accelerator and work day and night to get through.
There are some, however, who plan to enroll themselves in Delhi University for a postgraduate (PG) course as well. Thus, for those seeking admissions in PG courses, here's everything you need to know about the DU PG Admissions 2019:
First things first, the registration process, which is mandatory for all the candidates looking to seek admission in PG courses, has started from 3rd June 2019 onwards. The last date to register is 17th June 2019. You can register here.
Just like the undergraduate courses, there are plenty of postgraduate courses offered by the University of Delhi. Here's a complete list of courses offered:
Faculty of Arts: The faculty of Arts is offering M.A. in
Arabic,
Buddhist Studies,
French, German,
Hispanic, Sociology,
Economics,
Political Science,
Geography,
Sociology,
Social Work,
Hindi,
Linguistics,
Bengali,
Comparative Indian Literature, Tamil, Persian,
Psychology,
Applied Psychology,
Punjabi,
Sanskrit,
Russian Studies,
Urdu,
Environmental Studies,
Japanese, East Asian Studies, and
Faculty of Commerce: The faculty of Commerce is offering M.Com.
Faculty of Science: The faculty of Science is offering M.Sc. in
Biochemistry,
Food and Nutrition,
Human Development and Childhood studies,
Fabric and Apparel Science,
Physics,
Zoology,
Mathematics Education,
Genetics,
Microbiology,
Plant Molecular Biology, and
Biotechnology,
Statistics,
Anthropology,
Forensic Science,
Music: M.A. in
Carnatic Music,
Hindustani Music, and
Percussion Music.
1. Age –
There is no minimum age bar for admission to the Postgraduate Courses in the University and its colleges except in the courses where the respective regulatory bodies have prescribed the minimum age requirement in their respective regulations.
2. Qualifying Examinations –
The eligibility for sitting in the qualifying examination (i.e. the entrance test) varies from course to course.
Details on the same are given in Annexure – VI (from page number 54 onwards) of the PG Bulletin of Information 2019-20.
3. Reservation and Relaxation –
The University strictly adheres to Central Government Policies in matters of Reservation and Relaxation, and in order to avail the same under EWS / OBC / SC / ST quota a candidate has to produce a valid certificate issued by any of the regulatory authorities.
More details on this can be found in Section 2.4 (page number 18) of the PG Bulletin of Information 2019-20.
Also Read: Courses That Commerce Students Can Pursue If They Don't Meet DU's High Cut Offs
All the candidates seeking admission to the Postgraduate (PG) courses are required to register online.
There is a common Web Portal dedicated to the Registration of applicants and a common Application Form for admission to all the Postgraduate Courses in the University. Online Registration details are available on the following link of PG Admission Portal: http://www.du.ac.in.
In case you are a first time user, you will be asked to register on the portal to access the PG Admission Portal.
Remember to note both your username and password safely, because you will need it to access your account on the portal as well as for all the formalities to be completed during the admission process.
Once the registration is complete, login to the portal with your username and password and complete the application form.
You will be asked to upload a passport size photograph as well as a copy of an identity proof, which could be any of the following documents – Aadhaar Card / Voter's Identity Card / PAN Card / Passport / Driving License.
Once done, submit the application by clicking on the 'Submit My Profile' button at the bottom.
Thereafter, proceed with "Apply in New Course" and fill in all the required details, as well as the admission criteria (i.e. Merit-Based, Entrance-Based or Both, as applicable)
After selecting the course, save your application and proceed to pay the application fees. The application Fee can be paid through any one of the available online payment options: Net Banking, Debit Card, Credit Card, and UPI.
The application fee is as follows:
For Unreserved and OBC candidates: Rs. 750
For SC, ST, PwD and EWS candidates: Rs. 300
Take care to pay the application fees, since the application will be considered complete only after the realization of online payment.
Another important pointer to note is that in case you wish to apply in more than one course, you will have to fill a separate application form for each course and pay the application fee for the same.
Few more important pointers:
Fill the application form carefully, as any changes in the form after its submission is chargeable.
Application Fee once deposited will not be refunded/adjusted in any circumstances.
Mode of Admission
All the Departments of the University, except those that offer inter-disciplinary or professional courses, have two modes of admission for Postgraduate Courses:
MERIT-BASED: 50% of the total seats will be filled on the basis of the applicants' merit in the Qualifying Examination. Wherever applicable, MERIT BASED option is open only for the applicants who have eligible Bachelor Degree from the University of Delhi.
ENTRANCE TEST BASED: Remaining 50% of the total seats will be filled on the basis of applicants' rank in Entrance Test and Interview/Group Discussion, if any
Irrespective of the mode of admission, you will have to mandatorily register online on the admission portal, the process of which is explained above.
Each Department of the University will release separate Admission Lists in both modes.
For more information on DU PG Admissions 2019, go to the official DU website here or read the Bulletin of Information here.
Image Credits: Google Images
Sources: DU Information Bulletin
Find the blogger at @manas_ED
You'd also like to read:
As A DU Student, Here Are Things That Frustrate Me Most About My College
admission in delhi university
admission in du
admissions in postgraduate courses
delhi university admissions
Delhi University courses
Delhi University PG courses
DU Admission portal
DU PG Admissions 2019
Masters from DU
Previous articleWhat Skipper Virat Kohli Revealed Before India's First World Cup Match
Next articleThis Pride Month, Love Matters India Launches #AgarTumSaathHo, To Bring Together LGBTQ Community & Their Straight Allies
http://edtimes.in/author/manas-dixit/
I talk fast and read slow, so I write.
St. Xavier's College's Zephyrus Fest Celebrates India's Indomitable Resolve To Foster The Entrepreneurial Spirit
Verba Maximus 2023, BITS Pilani Hyderabad Campus' 11th Foray Into The Literature Verse
Girl Student At Lahore's American School Seen Assaulting Fellow Student In Video
Kerala Is Granting Maternity And Menstrual Leave To All Its University Students
E-Summit By IIT Hyderabad Looks At The Current Indian Startup Ecosystem & The Multiple Challenges It Face In Today's Time
In Mewat, A Woman's Activity On Social Media Gets Judged For...
Social Opinions February 1, 2023
Lifestyle February 1, 2023
LGBTQ Pitch On Shark Tank India 2 For & By Queer...


Mar 28, 2019 1:00 AM - I'm writing to express my support on the District 86 referendum for improvements to Hinsdale South and Central High Schools. I'm a father of two kids (8 and 2) and I moved to this town because of the schools. It's been 57 years since this district passed a referendum. These schools have 50-year-old boilers, classrooms are falling behind and the security systems are totally inadequate. Also, programs like wrestling, football, cheer, and the National Honor Society have all sustained cuts. Growing up, my high school experience was the best time of my life and now my kids are staring at their future and it looks pretty bleak.
Mar 24, 2019 1:00 AM - A Hinsdale letter to the editor: As a physician, I strongly oppose the Trump-Pence administration's gag rule.
Nov 2, 2018 1:00 AM - A Hinsdale letter to the editor: In endorsing Peter Roskam for Congress, the Daily Herald was reassured by his "inclination to act as a check on the president's more troubling impulses."
Oct 27, 2018 1:00 AM - A Hinsdale letter to the editor: There are many reasons to vote "yes" on Nov. 6 for the high school referendum, but I will keep this short and give you one of the main reasons-property value.
Oct 13, 2018 11:55 AM - In its endorsement of Republican Deanne Mazzochi over Jim Caffrey for Illinois House District 47, the Daily Herald believes her grasp of the issues will make her a strong voice in Springfield.


text:
Der Wahlkreis Erfurt, Land – Weimar, Land II war ein Landtagswahlkreis zur Landtagswahl in Thüringen 1990, der ersten Landtagswahl nach der Wiedergründung des Landes Thüringen. Er hatte die Wahlkreisnummer 19.

Der Wahlkreis umfasste den kompletten damaligen  Landkreis Erfurt mit folgenden Städten und Gemeinden: Alach, Alperstedt, Andisleben, Apfelstädt, Bienstädt, Büßleben, Dachwig, Döllstädt, Eckstedt, Egstedt, Elxleben, Ermstedt, Frienstedt, Gamstädt, Gebesee, Gierstädt, Großfahner, Großmölsen, Großrudestedt, Haßleben, Ingersleben, Kerspleben, Kleinmölsen, Klettbach, Kühnhausen, Linderbach-Azmannsdorf, Markvippach, Mittelhausen, Mönchenholzhausen, Molsdorf, Neudietendorf, Nöda, Nottleben, Ollendorf, Riethnordhausen, Ringleben, Rockhausen, Schloßvippach, Schwerborn, Stotternheim, Tiefthal, Töttelstädt, Udestedt, Vieselbach, Walschleben, Waltersleben, Windischholzhausen, Witterda, Zimmernsupra und Niedernissa
sowie Teile des damaligen Landkreises Weimar-Land mit folgenden Städten und Gemeinden: Ballstedt, Bechstedtstraß, Bergern, Bad Berka, Berlstedt, Buttelstedt, Daasdorf a. Berge,
Daasdorf b. Buttelstedt, Ettersburg, Gaberndorf, Gutendorf, Heichelheim, Hochdorf, Hopfgarten, Hottelstedt, Isseroda, Kleinobringen, Krakendorf, Krautheim, Meckfeld b. Bad Berka,
Neumark, Niedergrunstedt, Niederzimmern, Nohra, Obergrunstedt, Ottstedt a. Berge, Ramsla, Rittersdorf, Schwarza, Schwerstedt, Tannroda, Thangelstedt, Tiefengruben, Tonndorf, Tröbsdorf, Ulla, Utzberg, Vippachedelhausen und Weiden.

Ergebnisse
Die Landtagswahl 1990 fand am 14. Oktober 1990 statt und hatte folgendes Ergebnis für den Wahlkreis Erfurt, Land – Weimar, Land II:

Es waren 51.087 Personen wahlberechtigt. Die Wahlbeteiligung lag bei 76,3 %.  Als Direktkandidat wurde Volker Sklenar(CDU) gewählt. Er erreichte 51,1 % aller gültigen Stimmen.

Weblinks
 Wahlergebnisse nach Gemeinden

Erfurt
Politik (Landkreis Gotha)
Politik (Landkreis Sömmerda)
Politik (Landkreis Weimarer Land)
Politik (Erfurt)

redpajama_set_name: RedPajamaWikipedia


text:
Home News Education 202012
NUGS Commends NDC For Last Minute 'Fa Nyinyinaa' Manifesto Promise
/ Education, News
The National Union of Ghana Students (NUGS) has said a manifesto promise by the main opposition National Democratic Congress (NDC) to fully absorb fees for all fresh tertiary students admitted from the 2020/21 academic is commendable.
The association of graduate students said in a press release dated December 1, 2020, that the policy is a step in the right direction.
The NDC announced that it has incorporated free tertiary education for students admitted in the 2020/2021 academic year into its 2020 manifesto.
In a press statement issued on Tuesday, December 1, 2020, National Chairman of the party, Samuel Ofosu-Ampofo, said the proposal to update the manifesto was made by NDC's Education Policy Group.
Also, continuing students will pay half of their school fees under the updated manifesto promise.
According to the statement, the next NDC government will absorb "the full school fees of Ghanaian students who will be admitted to tertiary institutions in the 2020/2021 academic year."
"This policy is an enhancement of our commitment in the People's Manifesto to absorb 50% of the school fees of all tertiary students for the 2020/2021 academic year through the 'Kyemupe' policy, which will now apply to only continuing students in tertiary institutions in the country," the statement added.
The NUGS said in its statement said that it will hold the NDC and John Dramani Mahama, the party's flagbearer, accountable in fulfilment of this policy proposal if it wins the elections in December 7, 2020.
With days to the presidential and parliamentary elections, the NDC seems to be trying to outdo the incumbent New Patriotic Party (NPP)'s free Senior High School policy which gained grounds in the 2016 electioneering campaign.
Read the full statement from NUGS below.
NUGS Applauds Gov't For Tuition-Free Tertiary Education
150,000 Free SHS Graduates To Enjoy Govt Scholarship
WACCE Trains Over 1,800 Peace Ambassadors Against Violent Extremism In Ghana
University Of Ghana Ends Admission Process For 2020/2021 Academic Year
TTU Will Ensure Safety Of All Students On Campus – Registrar
16-Year-Old Girl Gets 6-Year Full Sponsorship For Medical School
Will I Send Ghanaian Kids To School In January 2021, No! Omane Boamah Writes...
Education In Retrospect
Universities Registration Improves
Parents Of Basic School Pupils Prepare Ahead Of School Reopening
Other Education Stories
Parents Dread Financial Implications Of Schools Reopening


Bridgeway Treatment Services is a unique Behavioral Health treatment facility that specializes in 'Mindfulness' combined with standard 'disease model' treatment. This format can be used in CD, MH or Co-Occurring (CO) CD & MH conditions to educate clients to better understand how their maladaptive self-destructive coping behavior is negatively affecting their lives. Bridgeway Treatment Services uses the evidence based practices of Dialectical Behavior Therapy (DBT) and Cognitive Behavioral Therapy (CBT) to address and identify overwhelming emotional states, as well as the skills necessary to self-regulate and address addiction and mental health triggers. This is accomplished by learning Mindfulness, Interpersonal Effectiveness, Emotional Regulation Skills and Distress Tolerance Skills as a way to deal with the stressors that plague people with addiction, as well as mental health issues. These concepts are integrated with the disease model to educate clients on how to develop an overarching holistic approach to building a recovery plan that will last a life time. This is how Bridgeway Treatment Services, LLC is "Bridging Addiction & Mental Health to Self-Discovery through Mindfulness".
Bridgeway Treatment Services is certified to provide the following Behavioral Health Services: Outpatient Mental Health, Substance Use Disorder, Mental Health Assessments, Drug & Alcohol Assessments, Individual Treatment, DUI Assessments, Group Therapy, Drug & Alcohol Information School (ADIS), Family Therapy Level I, Intensive Out Patient (IOP) (2yr Deferred), Brief Intervention Treatment Level II, Out Patient (Relapse Prevention), Case Management Level III and Out Patient (Monthly Maintenance).
Rather than focusing solely on addiction, holistic treatment facilities treat patients in terms of their whole being. Holistic treatment is about more than just addiction and sobriety – it addresses the person's life in its entirety, including career, physical, familial, and spiritual aspects.
Non 12-step programs promote a non-religious approach to rehab, and avoid calling addiction a disease (as traditional 12-step programs do). Instead, non-12-step programs emphasize scientific evidence when it comes to both treatment and recovery, and adapt to new addiction research as it comes out. Non-12-step programs stress the importance of self-reliance and empowerment (rather than dependence on a higher power, as in traditional 12-step programs). Non-12-step programs also tend to focus on co-occurring mental illnesses, if present. Addiction can make a mental health issue worse, and vice versa, so it's important to address both.
Dialectical Behavior Therapy (DBT) is a modified form of Cognitive Behavioral Therapy (CBT), a treatment designed to help people understand and ultimately affect the relationship between their thoughts, feelings, and behaviors. DBT is often used for individuals who struggle with self-harm behaviors, such as self-mutilation (cutting) and suicidal thoughts, urges, or attempts. It has been proven clinically effective for those who struggle with out-of-control emotions and mental health illnesses like Borderline Personality Disorder.
Eating disorders include anorexia, bulimia, binge eating, and dysfunctional eating patterns. Many psychologists and other mental health professionals consider eating disorders to be food addictions, meaning food is being used in an addictive way (similar to drug or alcohol addiction). Certain substance abuse treatment programs will have treatment for eating disorders as one of the services offered. An eating disorder may also present as a co-occuring disorder or dual diagnosis alongside drug and alcohol addiction.
Dialectical Behavioral Therapy is a cognitive behavioral psychotherapy developed to treat individuals diagnosed with Borderline Personality Disorder.
How effective was Bridgeway Treatment Services?


Using the example of the clamping lever for the automobile industry, the potentials of the chipless form production by means of powder press technology can be impressively shown.
The project was preceded by an enquiry from the automotive supplier industry asking whether there was any prospect of manufacturing a tension lever from iron powder. Until then the product had been extensively machined from a semi-finished part. The manufacturer came under serious price pressure as the end customer was intending to reduce the purchase prices as quantities increased. Using conventional production methods (machining), production took around 7 h/part. This might be feasible for the production of prototypes and small series but is quickly pushed to its limits for larger quantities.
In the second stage of the project, therefore, a semi-finished part was manufactured at the customer's plant using existing mechanical presses. Due to the pressing process, this part has already received the labor-intensive external contour of the plan view. The cylindrical shoulder and the latch in the lower region were machined after heat treatment (sintering).
As a result of this, the manufacturing time was shortened to around 1.8 h and productivity was increased. However, it emerged that after the launch phase this product would be manufactured in industrial scale quantities which are common for the automotive industry in the premium segment, e.g. 50 - 100,000 items/month. In an advisory meeting on site, Komage drafted the concept for the near-net shape press sequence jointly with the customer and also promised true-to-contour relaxation of pressure on the green part as a result of performing the pressing process without fixed stops.
As a minimum requirement for the number of units, it had to be possible to produce at least 4 parts per minute which would justify the investment in the new press system alone. As it turned out later, a series of program and tool optimizations ensured that this target was clearly exceeded.
The customer subsequently invested in an experimental tool, which was produced in collaboration with the engineering design departments. The very first setup and test on our pilot plant machine (a freely programmable hydraulic press of the S series) impressively highlighted the possibilities, and as a result led to intensive business relations between the companies involved.


text:
Entry of Alphaherpesviruses Mediated by Poliovirus Receptor-Related Protein 1 and Poliovirus Receptor
Robert J. Geraghty,
Claude Krummenacher,
Gary H. Cohen,
Roselyn J. Eisenberg,
Patricia G. Spear*
R. J. Geraghty and P. G. Spear, Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL 60611, USA.
C. Krummenacher and G. H. Cohen, School of Dental Medicine and Center for Oral Health Research, University of Pennsylvania, Philadelphia, PA 19104, USA.
R. J. Eisenberg, Center for Oral Health Research and School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Science 05 Jun 1998:
DOI: 10.1126/science.280.5369.1618
Robert J. Geraghty
Claude Krummenacher
Gary H. Cohen
Roselyn J. Eisenberg
Patricia G. Spear
A human member of the immunoglobulin superfamily was shown to mediate entry of several alphaherpesviruses, including herpes simplex viruses (HSV) 1 and 2, porcine pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1). This membrane glycoprotein is poliovirus receptor-related protein 1 (Prr1), designated here as HveC. Incubation of HSV-1 with a secreted form of HveC inhibited subsequent infection of a variety of cell lines, suggesting that HveC interacts directly with the virus. Poliovirus receptor (Pvr) itself mediated entry of PRV and BHV-1 but not of the HSV strains tested. HveC was expressed in human cells of epithelial and neuronal origin; it is the prime candidate for the coreceptor that allows both HSV-1 and HSV-2 to infect epithelial cells on mucosal surfaces and spread to cells of the nervous system.
Alphaherpesviruses, including HSV-1, HSV-2, PRV, and BHV-1, infect a variety of cell types in culture, resulting in efficient virus production in a short replicative cycle. Infection in the natural host is characterized by lesions in the epidermis, usually on mucosal surfaces, with spread of virus to the nervous system and establishment of latent infections in neurons. Binding of alphaherpesviruses to cells occurs primarily through an interaction of virion glycoprotein C (gC) with cell surface heparan sulfate, whereas fusion between the virion envelope and cell membrane requires the glycoproteins gB, gD, gH, and gL (1).
Several lines of evidence suggest that alphaherpesvirus gD interacts with a cell surface receptor in addition to heparan sulfate to mediate viral entry and that, in certain cell types, HSV-1, PRV, and BHV-1 can use a common gD receptor for entry (1, 2). Recently, a gD receptor for entry of HSV-1 and HSV-2 was identified as an additional member of the tumor necrosis factor receptor family, called herpesvirus entry mediator (3, 4), and is designated here as herpesvirus entry mediator A (HveA). HveA is the principal receptor for entry of HSV into human lymphoid cells but not into other cell types (3). Also, HveA fails to mediate the entry of PRV (3). A second mediator of HSV entry identified recently (5) was shown to be poliovirus receptor-related protein 2 (6). No function and no poliovirus receptor activity have been reported for this protein, and it is therefore designated herpesvirus entry mediator B (HveB). HveB mediates the entry of HSV-2 strains, PRV, and certain viable mutants of HSV-1 but fails to mediate the entry of wild-type HSV-1 strains or BHV-1 (5).
These results demonstrate that multiple alphaherpesvirus coreceptors exist, differing in their specificities for individual viruses in the subfamily. Neither HveA nor HveB fits the specifications for a coreceptor that can mediate entry of both HSV-1 and HSV-2 into epithelial cells at the initial site of infection and into neuronal cells for the establishment of latent infection. Also, neither HveA nor HveB serves as a coreceptor for all these viruses—HSV-1, PRV, and BHV-1—and therefore could not be a common coreceptor for these human and animal alphaherpesviruses. Because HveB is closely related to the poliovirus receptor (Pvr) (7) and to poliovirus receptor-related protein 1 (Prr1) (8), we explored the possibility that one or both of those proteins might mediate the entry of HSV-1 and -2 as well as PRV and BHV-1.
Chinese hamster ovary (CHO) cells express heparan sulfate chains, to which alphaherpesviruses can bind. However, CHO cells are resistant to the entry of HSV-1, PRV, and BHV-1 because of the absence of coreceptors required for virion cell fusion (3, 5, 9). CHO cells were transfected with plasmids expressing Pvr or Prr1 (10) and then inoculated with virus (11) to determine whether expression of the cell proteins could provide the necessary coreceptors for viral entry. Prr1 mediated the entry of several HSV-1 strains and three HSV-1 mutants (ANG, Rid1, Rid2) with amino acid substitutions in gD that preclude the use of HveA for entry (3) (Fig. 1). Prr1, designated here as HveC, also enhanced infection by HSV-2 strains (Fig. 1), although the enhancement was not as great because control CHO cells are partially susceptible to HSV-2 infection (9). HveC expression rendered CHO cells susceptible to PRV and BHV-1 as well as HSV, entry being a function of virus dose (Fig.2). Three independently isolated cell lines (CHO–HveC-1, -2, and -3) stably expressing HveC were also capable of being infected by PRV, BHV-1, and HSV-1 (12). Pvr mediated the entry of PRV and BHV-1 but not of the HSV-1 strains (Fig.2). The fact that HveC and Pvr, designated Pvr-HveD, can mediate entry of PRV and BHV-1 does not strictly imply that human cells could support the replication of those viruses; it does suggest, however, that the animal homologs of HveC and Pvr-HveD could mediate entry of those viruses into cells of the natural hosts.
Enhanced entry of HSV-1 and -2 strains into CHO cells expressing HveC. Subconfluent CHO-IEβ8 cells were transfected with a plasmid expressing HveC (pBG38, solid bars) or a control plasmid (pcDNA3, open bars) and 24 hours later replated in 96-well plates (about 2 × 104 to 4 × 104 cells per well). After 24 hours, the cells were incubated with virus at a range of concentrations and β-Gal activity was quantitated as a measure of viral entry, as described (3,11). The results depicted were for 50,000 pfu per well, in the linear range of plots of virus dose against β-Gal activity. Each experiment was performed with a subset of the viruses that always included HSV-1(KOS). Within each experiment, all values were made relative to the value obtained for the HSV-1(KOS)/HveC infection. Each virus was inoculated in triplicate; the mean values plus SDs for at least two separate experiments are shown.
Enhanced entry of HSV-1, PRV, and BHV-1 into HveC- and Pvr-HveD–expressing CHO cells. Subconfluent CHO-IEβ8 cells (3, 11) were transfected with plasmids expressing HveA (pBec10) (3), HveC (pBG38), Pvr-HveD (pBG42.16), or control DNA (pcDNA3). After 24 hours, the transfected cells were replated in 96-well plates (about 2 × 104 to 4 × 104 cells per well) and exposed the next day to HSV-1(KOS), HSV-1(KOS)Rid1, PRV(Kaplan), or BHV-1(Cooper) isolates expressing β-Gal (11). Six hours after inoculation, cells were lysed and β-Gal activity was determined as a measure of virus entry (11). The infections were done in triplicate and were repeated four times. The mean values plus SDs for a representative experiment are shown.
The nucleotide sequence of our isolate of HveC cDNA (GenBank accession number AF060231), as well as I.M.A.G.E. Consortium Clone 287663 (a fragment of HveC cDNA isolated from brain tissue) (13) differed from the originally published sequence (8) by the absence of single bases at positions 582, 597, and 617. The differences affected the amino acid sequence over a short range but maintained the overall open reading frame (14). Diagnostic restriction enzyme analysis of our clone, as well as the corresponding region amplified by polymerase chain reaction (PCR) from a HeLa cell cDNA library, verified our sequence (12). The hveC gene is located on human chromosome 11 (8), which is of special interest because a human gene capable of conferring to Chinese hamster lung cells a susceptibility to HSV-1 infection was previously mapped to human chromosome 11 (15). The genes for HveB and Pvr-HveD are located on chromosome 19 (6, 16), whereas that for HveA is on chromosome 1 (17).
To identify human cell types in which entry of HSV-1 might be mediated by HveC, we performed reverse transcription–PCR (RT-PCR) analysis with primers specific for HveC cDNA on total RNA isolated from human cell lines and primary cells (18). HveC mRNA expression was detected in NT2 cells (teratocarcinoma), SH-SY5Y and IMR-5 cells (neuroblastomas), HL-60 cells (promyelocytic leukemia), primary human diploid fibroblasts, primary human foreskin keratinocytes (Fig.3), and HeLa cells (12) but not in HEL299 cells (embryonic lung fibroblasts) or phytohemagglutinin-activated T cell blasts (Fig. 3) (18). As expected, expression of HveC mRNA was also detected in CHO cells that were stably expressing HveC cDNA but not in control CHO cells or in CHO cells stably expressing HveA or HveB (Fig. 3). RT-PCR performed with primers specific for HveA cDNA yielded the expected product in keratinocytes and T lymphoblasts but not in NT2, SH-SY5Y, or IMR-5 cells (12). NT2, SH-SY5Y, and IMR-5 cells are susceptible to HSV-1(KOS) infection (12) (Fig.4). HveC is the best candidate for the entry protein used by HSV-1(KOS) in these cells because expression of HveC, but not of HveA, was detected and neither HveB nor Pvr-HveD mediates HSV-1(KOS) entry. Although all four of the herpesvirus entry proteins are expressed in many human tissues and organs, expression of HveA is detected principally in lymphoid organs (17, 19), whereas HveB, HveC, and Pvr-HveD can be expressed in cells of the nervous system (5, 7) or in cells cultured from the nervous system (Fig. 3).
Expression of HveC mRNA. Total RNA was isolated from established cell lines and primary cells, the cDNA was obtained, and PCR was performed as described (18). (A) HveC mRNA expression in CHO lines stably expressing HveA (HveA-12), HveB (HveB-1), or HveC (HveC-1) and also the parental CHO cell line (K1) (18). (B) HveC mRNA expression in human cell lines as described in the text (18) and in primary cell cultures: HDF, human diploid fibroblasts; HuFK, human foreskin keratinocytes; HuTL, phytohemagglutinin-activated human T cell blasts (18). (C) β-Actin mRNA expression detected in the RNA samples from the corresponding lanes in (B) (18). The β-actin control results are also presented in a figure demonstrating HveB expression in these same cell lines (5).
Blocking HSV-1 infection by secreted HveA and HveC. HSV-1(KOS)tk12 was preincubated with BSA, HveA(200t), or HveC(346t) for 1 hour at 37°C. The cell lines (about 4 × 104 cells per well) were then exposed to the mixtures of virus and protein in 96-well plates for 1 hour at 4°C before transfer to 37°C for 6 hours. CHO–IEβ8/HveA (CHO-HveA) cells and CHO–IEβ8/HveC (CHO-HveC) cells were generated by transfection of CHO–IEβ8 cells (3) as described (21). Cells were lysed and β-Gal activity was quantitated as a measure of infection by HSV-1(KOS)tk12 (20). The values obtained for infections in the presence of added protein were compared with the infection obtained in the absence of added protein to determine percent of control. All values represent the average of at least two experiments performed in triplicate.
A secreted form of HveA, HveA(200t), binds to virus by an interaction with HSV-1 gD and blocks infection by HSV-1 (4). To ascertain whether HveC interacts with virion proteins to mediate entry, we incubated HSV-1 with HveA(200t) or with a secreted form of HveC, HveC(346t) (20), prior to infection. Incubation of HSV-1 with either of these truncated proteins inhibited infection of NT2 cells, IMR-5 cells, CHO cell lines stably expressing HveA (21) or HveC (22) (Fig. 4), and SH-SY5Y cells (23) in a dose-dependent manner. Either protein competed with membrane-bound forms of HveA or HveC for interaction with virus, thereby inhibiting infection. As expected, HveA and HveC were expressed on the surface of CHO–HveA-12 and CHO–HveC-1 cells, respectively, according to flow cytometric analysis (3, 12). Recent results demonstrate that HveC(346t) binds purified gD from HSV-1 and HSV-2 in vitro (22). Therefore, HveC(346t) may bind the gD present on the HSV-1 virion to inhibit infection, and HveC may be a receptor for virion gD.
Whereas HveA, HveB, and Pvr-HveD are active for entry of subsets of the alphaherpesviruses tested here, HveC mediated entry of all these viruses. Although the gD family members of the alphaherpesviruses share only 10 to 15% amino acid sequence identity, there is general conservation of the positions of six cysteine residues and probably conservation of a domain recognized by HveC. Substitutions at position 27 in HSV-1 gD abrogate entry via HveA (3) and enable entry via HveB (5) but have no effect on entry via HveC. These findings, coupled with the ability of HveA and HveC to compete for critical sites on virions to block infection, indicate that each entry protein may recognize overlapping but distinct structural domains of gD. Animal homologs of HveB, HveC, and Pvr-HveD probably are the principal coreceptors for entry of animal alphaherpesviruses and may be active for HSV strains, as predicted by experiments that identified gD coreceptors recognized by both animal and human alphaherpesviruses (2); however, this remains to be determined.
HSV-1 and HSV-2 strains exhibit differences in pathogenesis, some of which may be attributable to preferences for different entry receptors and, therefore, to targeting of different cell types. However, a common feature of all HSV-1 and HSV-2 strains, and of most alphaherpesviruses, is the ability to replicate in mucosal epithelia and to invade adjacent nerve endings, thereby establishing a latent infection in nerve cell bodies. The results presented here implicate HveC as the prime receptor allowing for HSV-1 and HSV-2 infection of mucosal surfaces and spread to the nervous system and suggest it as a prime target for innovative prophylactic or therapeutic interventions. Moreover, HveC homologs may serve a similar role for infections by animal alphaherpesviruses of their natural hosts and thus may account for the cross-interference patterns observed for human and animal alphaherpesviruses.
↵* To whom correspondence should be addressed. E-mail: p-spear{at}nwu.edu
P. G. Spear
, Semin. Virol. 4, 167 (1993); T. C. Mettenleiter, in Viral Vectors (Academic Press, New York, 1995), pp. 367–393.
G. Campadelli-Fiume, M. Arsenakis, F. Farabegoli, B. Roizman, J. Virol. 62, 159 (1988); E. A. Petrovskis, A. L. Meyer, L. E. Post, ibid., p. 2196; D. C. Johnson and M. W. Ligas, ibid., p. 4605; D. C. Johnson, R. L. Burke, T. Gregory, ibid. 64, 2569 (1990); A. Karger, T. C. Mettenleiter, Virology 194, 654 (1993); C. C. L. Chase, C. Lohoff, G. J. Letchworth III, ibid., p. 365; W. Lee and A. O. Fuller, J. Virol. 67, 5088 (1993).
R. I. Montgomery,
M. W. Warner,
B. J. Lum,
, Cell 87, 427 (1996).
J. C. Whitbeck, et al., J. Virol. 71, 6083 (1997); A. V. Nicola et al., ibid 72, 3595 (1998).
M. W. Warner et al., Virology, in press.
F. Eberle
, Gene 159, 267 (1995).
C. L. Mendelsohn,
E. Wimmer,
V. R. Racaniello
M. Lopez
M. T. Shieh
, J. Cell. Biol. 116, 1273 (1992).
The 5′ half of the Prr1 cDNA insert in pBG38 consisted of the Hind III to Bst EII fragment of the I.M.A.G.E. Consortium Clone 140737, obtained from a placenta cDNA library (13). The 3′ half of the Prr1 cDNA extended from the Bst EII site to the stop codon and was obtained by two rounds of PCR amplification of a placenta cDNA library (Clonetech) with use of primers int1 (5′-TCCTTCACCGATGGCACTATCC) and 108 (5′-ACACGTACCACTCCTTCTTG) for the first round and primers int1 and 104 (5′-GCTCTAGAGCGGCTACACGTACCACTCCTT) for the second round. The initial thermocycling conditions were 94°C for 1 min, 70°C for 1 min, and 72°C for 1.5 min. After every three cycles, the annealing temperature was decreased 3°C until 55°C was reached, after which cycling was continued to 35 total cycles. Two percent of the first PCR volume was used as template with the second primer set under the cycling conditions. The Prr1 cDNA was ligated into pcDNA3 (Invitrogen) via Hind III and Xba I sites. The Pvr cDNA (α form) was PCR amplified from a HeLa cDNA library (Invitrogen) as described (16), with primers pvr01 (5′-TCTGGAGCTTGAAGAAGTGGG) and pvr07 (5′-CACCTTGTGCCCTCTGTCTG) for the first round and pvr01 plus pvr08 (5′-CCTCTCAGTCCCGACGCTGT) for subsequent rounds of amplification. The Pvr cDNA was inserted into pcDNA3 via the Eco RV site to yield pBG42.16.
Infectivity assays were performed in 96-well plates as described (3). Subconfluent CHO-IEβ8 cells were transfected by using Lipofectamine (GibcoBRL). CHO-IEβ8 cells contain an immediate-early promoter of HSV-1 upstream of the Escherichia coli lacZ gene and express β-galactosidase (β-Gal) upon HSV entry, as a result of transactivation by VP16 (3). The viruses used for the infectivity assays in Fig. 2 were HSV-1(KOS)tk12 (5), HSV-1(KOS)Rid1-tk12 (5), PRV(Kaplan)gH− (24), and BHV-1(Cooper)v4a (25). These viruses express β-Gal from a lacZcassette inserted within their genomes. PRV(Kaplan)gH−virus was propagated on SW78 cells to obtain infectious virus. The description and references for the virus strains used in Fig. 1 were as described (3) except for HSV-1(mP) (26) and HSV-2(G) (27).
R. J. Geraghty and P. G. Spear, data not shown.
G. G. Lennon,
C. Auffray,
M. Polymeropoulos,
M. B. Soares
, Genomics 33, 151 (1996).
Nucleotide sequence analysis of pBG38 was performed by the University of Chicago Cancer Research Center DNA Sequencing Facility. The sequence differences were absences of cytosines at base pairs (bp) 582, 597, and 617 [numbered according to sequence published in (8)]. These changes yield a deletion of one amino acid residue and the resulting sequence Glu-Ala-Glu-Tyr-Gln-Glu-Ile-Arg-Asn-Pro-Asn-Gly-Thr-Val (amino acids 192 to 205) instead of Glu-Ala-Arg-Val-Pro-Gly-Asp-Ser-Gly-Thr-Pro-Met-Ala-Pro-Val (amino acids 192 to 206).
U. Francke and B. Francke, Somat. Cell Genet. 7, 171 (1981).
S. Koike et al., Embo J. 9, 3217 (1990).
B. S. Kwon
, J. Biol. Chem. 272, 14272 (1997).
SH-SY5Y cells were obtained from D. Pleasure and grown in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal calf serum. IMR-5 cells were also obtained from D. Pleasure and grown in RPMI medium with 10% fetal bovine serum. CHO K1 and HEL299 were obtained from American Type Culture Collection (ATCC) and grown as described (5). NT-2 and HL60 cells were obtained from ATCC and grown in DMEM and 10% fetal bovine serum. The primary human diploid fibroblasts were obtained from M. K. Rundell and the primary human foreskin keratinocytes were obtained from L. Laimins (28). The activated T cell blasts were prepared, and CHO–HveA-12 cells were isolated as described (3). CHO–HveB-1 cells have been described (5). Total RNA was isolated from 1 × 106 to 5 × 106 cells by using the RNeasy kit (Qiagen). The 3′ RACE kit (GibcoBRL) was used for RT. PCR amplification of HveC sequences was performed with int1 (10) and 105 (5′-TCAACACCAGCAGGATGCTC) primers and one round of thermocycling conditions (10). The primers int1 and 105 spanned three predicted exons; therefore, amplification of contaminating genomic DNA would result in a band of greater size than the HveC-cDNA–specific (738 bp) band detected. The β-actin control primers and thermocycling conditions have been described (29). The samples were subjected to electrophoresis on a 1% agarose gel and visualized by ethidium bromide staining.
H. Hsu
, J. Biol. Chem. 272, 13471 (1997); S. A. Marsters et al.,ibid., p. 14029.
HveC(346t) was truncated after amino acid 346 to yield a protein consisting of the predicted ectodomain of HveC, mellitin signal sequence at the NH2-terminus to facilitate secretion from insect cells, and a histidine tag at the COOH-terminus to facilitate purification from culture medium. Methods for construction of recombinant baculovirus that expressed HveC(346t) and purification of the secreted protein were similar to those described for HveA(200t) (4 , 22). Blocking experiments were carried out in 96-well plates (about 4 × 104 cells per well). Serial dilutions of HveA(200t), HveC(346t), or bovine serum albumin (BSA) were mixed with virus solution [4 × 105 plaque-forming units (pfu) of HSV-1(KOS)tk12 per milliliter (5), 30 mM Hepes, DMEM, and 10% fetal calf serum]. The mixtures were incubated at 37°C for 1 hour and then chilled on ice. The cells were chilled at 4°C for 10 min before infection. Protein and virus mixture (100 μl) was added to each well and incubated at 4°C for 1 hour, after which the plates were transferred to 37°C for 6 hours. Cells were lysed by adding to each well 100 μl of culture medium containing 1% NP-40. Fifty microliters of cell lysate was transferred to another 96-well dish, 50 μl of chlorophenol red-P- d -galactopyranoside was added, and the kinetics of β-Gal activity was measured at 570 nm.
T. Terry-Allison et al., J. Virol., in press.
C. Krummenacher et al., ibid., in press.
C. Krummenacher, G. H. Cohen, R. J. Eisenberg, data not shown.
N. Babic
, J. Gen. Virol. 77, 2277 (1996).
J. M. Miller,
C. A. Whetstone,
L. J. Bello,
W. C. Lawrence,
J. C. Whitbeck
, Am. J. Vet. Res. 56, 870 (1995).
M. D. Hoggan,
B. Roizman
, Am. J. Hyg. 70, 208 (1958).
J. M. Ejercito,
E. Kieff,
, J. Gen. Virol. 2, 357 (1968).
J. T. Thomas,
L. A. Laimins
, J. Virol. 72, 1131 (1998).
J. C. Willey
, Am. J. Respir. Cell Mol. Biol. 14, 262 (1986).
We thank T. Mettenleiter for PRV(Kaplan)gH−, L. Bello for BHV-1v4a, C. Waltenbaugh for use of his spectrometer, N. Susmarski for work with cell lines and virus stocks, W. Hou for technical assistance, and R. Xu for CHO-IEβ8/HveC cells. We also thank R. Montgomery, M. Warner, and P. Speck for helpful discussions, and R. Longnecker and L. Laimins for suggestions on the manuscript. Supported by a grant from the National Institute of Allergy and Infectious Diseases (RO1 AI 36293 to P.G.S.) and by grants from the National Institute of Neurological Disorders and Stroke (NS-36731, NS-30606, and AI-18289 to R.J.E. and G.H.C.). R.J.G. is supported by National Research Service Award F32 AI09471.
You are going to email the following Entry of Alphaherpesviruses Mediated by Poliovirus Receptor-Related Protein 1 and Poliovirus Receptor
By Robert J. Geraghty, Claude Krummenacher, Gary H. Cohen, Roselyn J. Eisenberg, Patricia G. Spear
Science 05 Jun 1998 : 1618-1620


text:
Leicester selected
Passenger killed in crash in Humberstone
https://www.bbc.com/news/uk-england-leicestershire-11646080
A 64-year-old woman died when the car she was a passenger in collided with another vehicle in Leicestershire.
The crash happened in Colchester Road in Humberstone at the junction with Elmcroft Avenue on Wednesday afternoon.
Valerie Dunkley, of Leicester, was a passenger in a Rover Metro which collided with a Porsche Carrera. She died at the scene, police said.
The Rover driver was taken to the Leicester Royal Infirmary where he remained in a serious condition.
The male driver of the Porsche was treated in hospital for minor injuries.
Det Con Rob Lapworth said: "The Porsche was travelling southbound along Colchester Road towards the A47 Uppingham Road and the Rover was travelling in the opposite direction when the collision occurred.
"I would appeal to anyone who was travelling along these roads yesterday afternoon to contact us if they witnessed the collision or saw either vehicle beforehand. Any information, no matter how small, could help us with our investigation."
Leicestershire Constabulary
Trump impeachment trial: Latest updates


As an independent wedding celebrant in the region, I have had the pleasure of working with wedding and event planners throughout area, conducting ceremonies in a number of venues and meeting many wonderful wedding couples.
The Algarve is the perfect setting for your wedding ceremony. I particularly love the dramatic outdoor ceremonies with the vivid blue sky providing the ultimate backdrop. Originally from the UK, I have lived in the Algarve in Portugal since 2004 and I call it home.
My background in public speaking and working with people, provides a good grounding for the role, but I believe that the greatest strength a celebrant can offer is to help put couples and their family and friends at ease. Ensuring this is the most important part of my role and something I work hard to achieve.


Gosh, this one is chutney queen's fav - a savoury one it is lip-smakingly good. How someone described it at a market - "it's explode in your face kinda good".
Fav sourdough toastie recipe (chutney, cheese and poached chicken). With a dhal curry. Boil some rice and few spoons of this epic, lip-smacking chutney. Few blobs on your home made pizza.


In Canada, the weather in September feels a whole lot different than it does in January or February. Classrooms have a different feeling to them as well: at the start of the school year it seems like there is so much time to get everything done, to cover all of the learning expectations for the year or semester. As educators, we know all too well that it doesn't take long for that feeling of "abundance of time" to quickly evaporate. Part way through the year, it already feels like a mad dash to cover everything left in the curriculum. We often can't afford to lose even one instructional day, and then in the winter months along comes the 'Snow Day'.
Many northern school districts will experience inclement weather days, or "snow days", where school buses are cancelled and school may be closed for days at a time. Even the best laid unit plans can be totally derailed when a snow day hits.
It is challenging to miss out on teaching days given the amount of curriculum content we have to cover. The number of snow days that happen each year fluctuates, but no matter what, those lost days have a huge impact on getting through the class material, leaving us feeling that there is not enough time to accomplish it all. The solution to this is of course a digital one.
In the first days of the school year, we let our classes know that the expectation on an unexpected snow day is that students should log into their school Gmail accounts and check for an email from their math teacher about the lesson for that day (as a teacher with Hapara, you can easily send this email to your class from the Class Info Tab). If the weather network is predicting a heavy snowfall or some freezing rain in the coming days, then we remind students ahead of time to check their Hapara Workspace for the learning expectations for that day's lesson. Forget about missing a full instructional day, sometimes it's like our class doesn't even miss a beat!
Workspace is a good tool for organizing the various elements of any particular topic. It makes room for posting a clear learning goal (something like: "I will be able to calculate the missing interior angles of all polygons") as well as learning resources. The content of the lesson can be delivered in a number of ways online, many of which will provide a digital learning experience that continues the momentum for student engagement with the material.
Sometimes, when there is a brand new concept that needs to be taught, the teacher will create a short video explanation, or find a similar link on Youtube, where they can share the appropriate links with the students. Other teachers have run a live screen-capture chat with an almost full class of students on the popular video-game streaming website Twitch. And others still will decide to do an 'App-Smash' and get students to film themselves and explain their thinking using a program called Flipgrid. Most of the time, we will just have the prepared notes for the lesson, with examples and explanations all included.
The idea is that students will be at home all day with not much else to do anyway, so they can certainly spare an hour or two to investigate the content of their math lesson, and then work on some of their own questions and problems to solve. In Workspace, teachers can assign an Evidence task as an exit-card or a "Did You Get It?" mini-quiz. Often we will provide the solutions so that the students can check their own results and make sure that they are on track.
If students have a question or require a clarification about something specific, they are sometimes encouraged to email the teacher directly, before the school day ends – but some teachers have created a Google Doc for their whole class that students can all edit and post their questions there, for everyone to see. So often if one student has a question, then there are several others that had the exact same question — this way everyone can benefit from the answer.
Using Hapara with G Suite and a variety of other apps has helped our school board and especially my math department move the students into a blended-learning environment in a way that has been quite seamless for students, while also helping teachers decrease their anxiety of lost time due to inclement weather. As educators we always need to be flexible, and leveraging digital is one great way to make sure that the deep learning continues, even on a snow day!


Marissa Rundell filmed an angry passenger yelling at a flight attendant after being seated next to Rundell and her 8-month-old son.
A woman on her way to Syracuse found her trip cut short after she was booted from the plane for refusing to sit next to a crying baby.
The child's mother, 19-year-old Marissa Rundell, told her story on Facebook earlier this month, and the video she posted of the angry passenger has quickly gained popularity online.
During an Endeavor Air flight from New York's JFK Airport to Syracuse's Hancock Airport, Rundell, who was traveling with her 8-month-old son, says that the unidentified woman was the last to board the plane and began causing a ruckus even before she was actually seated. The woman allegedly began complaining about having to sit in the back of the plane, Syracuse.com reports, calling it "f*cking ridiculous" and slamming her bags down. Rundell says that, after she asked the woman not to use that type of language around her son, the woman responded by telling her to "shut the f*ck up and shove it."
Rundell says that the woman then started complaining about her seat, yelling, "I'm not sitting next to a crying baby!" Rundell responded by telling her that her baby wouldn't be crying during the entire trip, but the woman insisted that she would not sit next to a crying baby. When a flight attendant then informed her that she could take the next flight, she refused and asked for the employee's name — Tabitha — and ID number.
"Thank you, Tabitha," the woman reportedly said. "You may not have a job tomorrow."
When Tabitha then told another member of the crew that she wanted the unruly passenger removed from the flight for "screaming at the baby," the woman changed her tune, apologizing and explaining that she was "really stressed out." Unfortunately for her, her apologies didn't save her travel plans — she was kicked off the flight anyway, and Rundell shared video of the incident on her Facebook page.
"This lady thought she was going to be rude to me and Mason now she has no way home today. Thank you to the lovely Delta flight attendant for not letting this women bully us," Rundell wrote, adding, "Karma is a b*tch."
Her video has been viewed over 1.4 million times. Check it out below.


text:
package org.snf4j.core.allocator;

import java.nio.ByteBuffer;

/**
 * Allocates and manages {@link ByteBuffer}s used by the sessions for I/O
 * operations.
 *

 * The implementation should be thread safe.
 *
 * @author SNF4J.ORG
 */
public interface IByteBufferAllocator {

	/**
	 * Tells whether or not this allocator allocates buffers that are backed by
	 * an accessible byte array.
	 *
	 * @return true if the buffers allocated by this allocator are backed by an
	 *         accessible byte array
	 */
	boolean usesArray();

	/**
	 * Allocates a new byte buffer.
	 *
	 * @param capacity
	 *            The new buffer's capacity, in bytes
	 * @return The new byte buffer
	 */
	ByteBuffer allocate(int capacity);

	/**
	 * Tells if an implementation of the allocator supports releasing of no
	 * longer used buffers.
	 *
	 * @return true if the releasing is supported
	 */
	boolean isReleasable();

	/**
	 * Informs that given buffer that was allocated by this allocator is no
	 * longer used and can be released.
	 *
	 * @param buffer
	 *            buffer to be released
	 */
	void release(ByteBuffer buffer);

	/**
	 * Ensures that returned buffer will contain some room for new data. The
	 * content of the original buffer must be preserved in the returned buffer.
	 * If the original buffer is full and its capacity reached the max capacity
	 * the method should throw an {@link IndexOutOfBoundsException}.
	 *

	 * It is only used to manage the capacity of the input buffer associated
	 * with the stream-oriented sessions.
	 *
	 * @param buffer
	 *            buffer in the write mode (i.e. not flipped yet)
	 * @param minCapacity
	 *            min capacity
	 * @param maxCapacity
	 *            max capacity
	 * @return the original buffer or new one in the write mode.
	 * @throws IndexOutOfBoundsException
	 *             if the buffer is full and its capacity reached the max
	 *             capacity
	 */
	ByteBuffer ensureSome(ByteBuffer buffer, int minCapacity, int maxCapacity);

	/**
	 * Assures that returned buffer will contain room for new data. The
	 * content of the original buffer must be preserved in the returned buffer.
	 * If there is no room in the buffer for the new data the method should throw
	 * an {@link IndexOutOfBoundsException}.
	 *

	 * It is only used to manage the capacity of the input buffer associated
	 * with the engine driven stream-oriented sessions.
	 *
	 * @param buffer
	 *            buffer in the write mode (i.e. not flipped yet)
	 * @param size
	 *            size of data to be put to the buffer
	 * @param minCapacity
	 *            min capacity
	 * @param maxCapacity
	 *            max capacity
	 * @return the original buffer or new one in the write mode.
	 * @throws IndexOutOfBoundsException
	 *             if there is no room in the buffer for the new data
	 */
	ByteBuffer ensure(ByteBuffer buffer, int size, int minCapacity, int maxCapacity);

	/**
	 * Tries to reduce the capacity of the buffer based on its content. The
	 * content of the original buffer must be preserved in the returned buffer
	 *

	 * It is only used to manage the capacity of the output buffers associated
	 * with the stream-oriented sessions.
	 *
	 * @param buffer
	 *            buffer in the write mode (i.e. not flipped yet)
	 * @param minCapacity
	 *            min capacity
	 * @return the original buffer or new one in the write mode.
	 */
	ByteBuffer reduce(ByteBuffer buffer, int minCapacity);

	/**
	 * Extends the capacity of the buffer based on its current capacity. The
	 * content of the original buffer must be preserved in the returned buffer.
	 *

	 * It is only used to manage the capacity of the input buffer associated
	 * with the datagram-oriented sessions. The method is called after receiving
	 * a datagram that fully filled up the input buffer.
	 *
	 * @param buffer
	 *            buffer in the write mode (i.e. not flipped yet)
	 * @param maxCapacity
	 *            max capacity
	 * @return the new buffer
	 */
	ByteBuffer extend(ByteBuffer buffer, int maxCapacity);
}

redpajama_set_name: RedPajamaGithub


text:
Himachal Pradesh Cabinet directs to check the illegal mining and smuggling of medicinal herb Nag Chhatri
Nov 09, 2012The News HimachalNewsComments Off on Himachal Pradesh Cabinet directs to check the illegal mining and smuggling of medicinal herb Nag Chhatri
Himachal Pradesh Cabinet in its Meeting, presided by Chief Minister Prem Kumar Dhumal, approved to check the illegal and unscientific mining in different river beds and forest areas all over the State to protect environment and other hazards emerging out of such activities by directing the concerned authorities to take effective steps and stern action against the errants, especially in the forest areas, under the prevalent rules and regulations enacted for the purpose by the State Government.
In its meeting, Himachal Pradesh Cabinet also takes a serious note on smuggling of 'Nag Chhatri,' 'Trillium Govanimum', a perennial medicinal plant found in the temperate zone of the State, and directed to check effectively, so that its indiscriminate and excessive exploitation that is posing a serious threat to it, will be checked and stopped it at the movement.
Cabinet also granted approval to the Market Intervention Scheme (MIS) for Citrus Fruit during 2012-13 to procure Kinnow, Malta, Santra and Galgal etc. fruit from the growers by designated agencies of HPMC, APAIC and HIMFED through 54 procurement centres proposed to be set up in fruit growing areas all over the State.
Out of the estimated total produce of 27,395 MTs. 500 MTs. Kinnow/Malta/Santra and 100 MTs. of Galgal is proposed to be procured under the Scheme. The B grade Kinnow/Malta/Santra shall be procured at a rate of Rs. 6.00 per kg, C Grade at Rs. 5.50 per kg. and Galgal at Rs. 4.50 per kg. Designated agencies shall be entitled to get Rs. 2.65 per kg. as handling charges for procurement of Kinnow/Malta/Santra while for Galgal a rate of Re. 1.00 per kg. shall be paid. The Scheme shall be implemented during the period from November 20, 2012 to February 15, 2013.
In the meeting it was decided to provide 10,000 MTs or actual apple procured under MIS during current apple season, to HPMC free of cost for production of apple juice. It was approved to allow HPMC actual sale price of Rs. 1.46 per kg. and HIMFED Rs. 1.57 per kg., against the estimated price of Rs. 3.00 per kg. for the apple procured under MIS during the apple season of 2010, when the State had record apple crop.
himachal enws Himachal Pradesh Cabinet Nag Chhatri Prem Kumar Dhumal
Previous PostHP University's annual exams to start from March 15 Next PostLalita Vakil awarded 'Shilp Guru Award' with elation from the Governor and CM
BJP wins the battle but fails to choose its leader
Himachal Elections 2017: BJP wins 44 seats, Congress 21
Himachal Elections 2017: PM asks people to bid farewell to corrupt Govt


Over the last six months I have given a tremendous amount of thought to starting a new blog that better reflects my current take on "life, the universe and everything" than my build peace blog. Everytime I would find the perfect domain name for a new venture and begin to build a new whirling mass of me-ness in cyberspace I'd find that I missed this blog into which I had put so much energy for so many years. Please allow me to be a bit self-reflective here and a bit self-reflexive too as I deconstruct my thought processes about writing and blogging about inner peace, world peace, paths to peace, tools of peace, tools for peace, and… well you get the idea.
I have to admit I heaved a huge sigh of relief the evening of the election, Tuesday November 4th, when I gathered with a couple thousand other Tucsonans to watch the returns come in at the University Marriott. I will never forget standing next to a long retired colleague of my husband who came to the U of A after being booted from the California University system during the highly political days of the free speach civil rights era turbulence of the early 1960s and who helped establish ACLU to this Old West enclave. As McCain was conceeding defeat I happened to see this man's face as complete and utter joy washed over it.
At that time I decided that I was going to take a couple days off for R & R. I ended up taking off six months. Long story, short version: I had continuing pain after a bout with the stomach flu and ended up finding out I have Non-alcoholic Fatty Liver between Thanksgiving and Christmas. After my esteemed scientifically-gifted genius research professor husband (he might read this, lol) looked at the literature, we think that the combination of anti-depressants I've been on for many years could have contributed to the development of this condition as well as my being significantly overweight. It could relate to the invasive and often toxic medical tests and treatments I underwent as a child. I never had hepatitis nor am I an alcoholic.
I don't do well with being ill. It makes me depressed. I have been working on, and off — a lot of off, some on — on a book about my childhood experience on the receiving end of my mother's instability that I have come to realize falls into the range of behavior that has been termed Munchause Syndrome by Proxy. At least partially due to being "sickly" and kept out of school and shuttled from doctor to doctor when I was little so that it was not uncommon for me to miss up to 100 school days a year during my primary education.
My journals during the teen years are painful to read but allowed me as a middle-aged adult to see that my isolation during my childhood coupled with being thrust into the socially topsy-turvy, psychedelic and sexually liberated world of the 1970s as a totally naive, socially incompetent, pathologically shy human being were the primary factors that contributed to multiple rapes, exploitation, and emotionally abusive relationships, and less than wise choices that pretty much characterized my teens and young adult life.
Writing helped save my life time and again when the blank page or screen was the only tool through which I could communicate – even if that communication was basically just me talking to myself. I became fairly good at this apparently, and the winning of a state-wide writing competition in High School allowed me to win scholarships that made it possible for me to attend college. Writing continued to be my main communication channel with the world as I wrote essays, exams, reports and post-graduate research reports and theses.
Then the internet evolved into the web and I reached out through words and found that while I was a social mutant (and mutations can be a very good thing that allow the process of evolution to happen) I was also thinking things and making observations that other people found interesting and sometimes even amusing when I wrote about them. I was blogging long before a thing called a web log existed.
Then CODEPINK and I found each other. This blog, Build Peace, has been the most consistent and meaningful body of my writing to date. It allowed me to enter the BlogHer community of women's online voices.
A few days ago I was talking to a Second Life friend about my dilemma of attempting to shift my peace activism and writing from a broader and more tempered perspective than I had in Build Peace. She said, "Repurpose the blog." Initially I thought, "Ugh, corporate speak. Yuck. Ptooey." But then, trying to live the peace and be the peace I write about, I decided that I can continue writing/blogging about info that helps me build my own peace and share that with others that is the only way peace in our world will ever be built – person to person, one word at a time…. and with my bias showing I will further state that a sustainable peace must be built by women talking to each other and creating the world we want for our children.
Words like emergence, energy work, calmness, spiritual, belief, attraction and the like will be far more common here than in the past. I will talk about my struggle to lose weight, the challenge of shifting to an empty nest at home, and about healing. Politics will undoubtedly creep in… I've been political since I was about 10, but I will try to mediate my outrage and use words like Fascist and F**k far less often and perhaps not at all from this time forward. Maybe I can even get the Huffington Post to end their ban on links to my site in their comments. Purportedly this is because of profanity. We'll see.
Anyway, consider this blog repurposed. The appearance will change dramatically. The content may too. Peace to you all.
« I'm thankful for the brave women of the peace movement.
No Prescription medication Pharmacy. Get Cheap Drugs online. Buy Pills Central.


text:
Owen Coyle: The players are hungry for success
ISL Media
Rutvij Joshi
The Marina Machans will be looking for their third title in the ISL on Saturday night.
ATK FC take on Chennaiyin FC in the final of the sixth edition of Indian Super League at Fatorda Stadium in Goa on Saturday. In the short history of the league, both the teams have tasted success twice and will be looking to grab their third ISL title. However, the game is set to be played behind closed doors due to the coronavirus outbreak in the country. Before the grand finale, Chennaiyin's head coach Owen Coyle and captain Lucian Goian attended the press conference and addressed the media.
The former Burnley manager started the presser by giving his thoughts on the season and the ISL final. He said, "First and foremost, we as a group have worked hard to reach the final and we are not here by accident, we are here because we've earned it."
"When I came here, we were in a difficult situation, we had to win every week because teams like Mumbai and Odisha were winning games and also FC Goa, ATK and Bengaluru FC were the top three. They had set the bar and everybody else was trying to grab the fourth spot. But, the team deserves credit to win key games and have a mentality to adjust to the situations. We kept the disappointment of the NorthEast game behind and started focusing on the next game."
Mentality ahead of the final
"The mentality doesn't change even when we reached the playoffs. We were under pressure against FC Goa and everybody thought that they could win it because they were the #1 team for a reason. But, since January, we've enumerated the most points than anybody else in the league. We knew we could win the game. We respected them, but we never feared them and tomorrow we go with the same attitude. We are under pressure because we want to be champions because we've worked hard and we have got desire. The players are hungry for success and ATK will also be trying to win the game," Owen Coyle quoted.
Team atmosphere after Owen Coyle took over
The Chennai-based outfit had a difficult campaign last season. Yet, they decided to retain their then head coach John Gregory for another season, but things didn't change and John Gregory left the club. Owen Coyle came as his replacement and since then, the Marina Machans managed to do the unthinkable and reach the final.
Lucian Goian discussed the Englishman's influence as he went on to say, "When he came, he changed the way of training and also changed our attitude to training. I've seen in football that the way you train is the way you play. He gave confidence to some young players who were down at the start of the season and some positive results at the beginning contributed to the confidence."
"We became a much stronger group because we started to know each other well. At the start of the season, we were 25 new players and we didn't know each other. We didn't know our characters, we didn't know when to make ourselves better and make connections between us. In the second part of the season, we showed we are a very strong group and we deserved to be here," he added.
Lucian Goian signed up for the Marina Machans in the summer from Mumbai City on a free transfer and he shared his thoughts about the transfer, "I knew what happened last year with them and I thought that they will learn from their mistakes. The team were the two-time champions and it was a different challenge for me. Now, I look back to it and think I have made a wonderful choice."
Third ISL final in six years
Chennaiyin FC are playing their third Indian Super League final in six years and club captain Goian said it was the perfect opportunity to be part of the club's history. "I told the boys that tomorrow we are going to be part of the history of this club. If we move out nobody will remember us next year, but if we win the game and win the final then people will remember us after 10 years. Some players will win the third title with the club and they will be part of the photos of the club."
Coaching in English Championships and ISL
Owen Coyle has prior experience of coaching in the final with English Championship side Burnley, when they won promotion to the Premier League. He was asked about the difference in coaching in India and England. "Coaching in ISL is simple in many ways. We have fantastic a group of players, I remember we played 61 games that season and we had a financial embargo too. There are many similarities between the Championship and the ISL."
Related News | ARTICLE CONTINUES BELOW
Preview: Bengaluru FC meet Chennaiyin in high-stakes Southern Derby
Top five youngsters in ISL 2022-23 so far
Simon Grayson: Aim is to play with confidence not arrogance
Neither of the teams have reported any additional injuries over the last few weeks, meaning that they're both likely to be at full strength for the final.
While the side from Kolkata currently edge the Marina Machans in terms of head-to-head, it's interesting to note that both the league fixtures between the sides this season ended in away wins.
Predicted Lineup
Kaith, Renthelei, Sabía, Goian, Jerry, Vanspaul, Thapa, Chhangte, Crivellaro, Schembri, Valskis.
The final of the Indian Super League can be viewed on Star Sports 2 and Star Sports 2 HD. It's also available for viewing on Hotstar and Jio TV on other streaming platforms.
For more updates in Indian Football, follow Khel Now on Twitter.
Antonio López HabasATKChennaiyin FCISL-Indian Super LeagueLucian GoianOwen CoyleRutvij Joshi


Check out this Hindi movie . Hindi Movie New HD Movies in Hindi to Watch . 4. Salaam Bombay (1988) The movie directed by Mira Nair and the stars are Shafiq .
Bombay Velvet 2015 Full Movie Watch Online Free Download HD . Watch Online Bombay Velvet 2015 Full Movie in HD . love, greed, and jazz Bombay Velvet is the .
Dhoom 4 Full Movie Hd 1080p Watch or download movies . ninja 2 full hd movie in hindi download; . tamil a to z movie hd download; jorny bombay to goa full movie .
Hate Story 4. Baaghi 2. . Shahid has revealed that he bonded with the actor over their common love for music. .


Social Finance is a not-for-profit organisation that works in partnership with government, funders and the social sector to achieve sustainable social impact at scale. Since we started in 2007, our best-known innovation, the Social Impact Bond, has spread to 25 countries.
In the UK, we work on driving social change across issues such as homelessness, unemployment, mental health, education and vulnerable children. Internationally, we design results-based models for issues such as maternal health, education, employment and waste management.
Our health and employment team is committed to helping people with health issues or disabilities to attain and sustain fulfilling, paid employment. We have been working for over five years to help grow high-quality health and employment services, in particular those that adopt the best practice Individual Placement and Support (IPS) approach. IPS helps people with serious mental illness to find and keep a job that matches their preferences. It has an international track record of delivering outstanding outcomes. We are passionate about its potential to change lives.
We are currently working with NHS England and other partners to rapidly expand the number of people with serious mental health issues who can access these services through a programme called IPS Grow.


The Texas Motor Speedway Dirt Track is home to multiple events throughout the year including ASCS Sprint Car, Limited Modified and Factory Stock car races as well as Monster Truck and motorcycle events.
The Dirt Track is a clay surface, four-tenths of a mile, banked 12 degrees and is located adjacent to the backstretch of Texas Motor Speedway. The grandstands seat just under 11,000, plus another 300 in the luxury suites.
Call (817) 215-8500 for information or click here for the next scheduled event at the Texas Motor Speedway Dirt Track.
Click any image below to see what it's like to attend a dirt track event at Texas Motor Speedway.