text:
Slant 10-9-2014
Slant by EW StaffPosted on 10/09/2014
• National sportscasters were speculating about Phil Knight's giant gift to the UO during the losing Duck football game, so it must be time for a local announcement. The rumor we've heard is that $1 billion from the Knights will go the UO this month with some kind of match requirement. If true, that could help attract a superman or superwoman president, or maybe not.
• Kevin Matthews lost to incumbent County Commissioner Faye Stewart in the May Primary by a 14-point margin (2,069 votes) but has launched a write-in campaign as a protest to Stewart's poor environmental record representing the East Lane District. In a much closer race, Dawn Lesley lost by a just a tiny fraction of a point (74 votes) in her effort to unseat Commissioner Jay Bozievich in West Lane, but Lesley is not asking for a write-in vote. Lesley made that decision soon after the long recount, saying resources would better "be channeled into a strategic, winnable effort," and she's focusing on helping the Jeff Merkley Senate race. Lesley learned a lot from the campaign, and is left in a good position to run again in 2017. Meanwhile, Bozievich continues to build on his record of regressive decisions.
Matthews tells us his campaign is a "long shot," but he's bothered by Stewart ending up unchallenged on the November ballot with only 17 percent of the registered voters giving him the nod in the primary. He says the grassroots "Write in Kevin" campaign "gives a way to express how we feel, for all of us not satisfied with the top-down crony capitalism of the Lane County incumbents." He's added bright yellow "Write In" stickers to his lawn signs. To help in his campaign, email info@friendsofkevinmatthews.org.
• Think "authenticity." That's our word to describe Oregon Sen. Jeff Merkley and his guest, Massachusetts Sen. Elizabeth Warren, who rallied progressive Democrats in Eugene Oct. 6 in the EMU on campus. She was here to boost his re-election on Nov. 4 against Republican Monica Wehby, not so authentic. Merkley is the son of a millworker from southern Oregon, Warren is the daughter of an Oklahoma janitor. Both talked about rescuing America's middle class, including students, from debt, declining standard of living and the loss of purchasing power. We were struck that this should be the agenda of conservatives in this country. Warren radiated that rare talent for connecting with her listeners often demonstrated on both the Bill Maher and Jon Stewart shows. They love her and so did the Eugene crowd.
• The Oregon Drive Less Challenge began this week, a statewide effort to get Oregonians to cut 1 million miles from their driving over the next two weeks (see drivelessconnect.org). This week also saw a press conference by BEST (Better Eugene-Springfield Transit) calling on local leaders and the community to "respond to transit challenges and opportunities." BEST President Eric Gunderson says the organization has had more than two dozen community meetings and "hundreds of individuals said that transit is important to the community's people, economy and environment." Other speakers touted public transit's importance to low-income residents, students, the elderly and alter-abled, and to people who just want to live more sustainably. BEST will release its final report in January with recommendations on "how the community can move forward together in making our good transit system ever better for all," Executive Director Rob Zako says. We appreciate what BEST is doing, particularly since the whole concept of public transit is under attack locally and nationally.
• Get us to our wonderful farmers markets for riches resulting in part from record-breaking heat, a rare climate change plus. Local tomatoes, heritage and ordinary, are still stacked, along with strawberries picked that morning. Grapes have never been sweeter. Pears. Winter squash. A pallet of peppers, red, yellow, orange, green, some roasting while we watch. Quince, the awkward fruit itself, and organic quince butter to be eaten with cheese on a cracker, at the Bergs stand at the downtown market.
Slant 3 days ago
The earnest but sometimes tone-deaf Shedd Institute for the Arts generated heated criticism over the weekend after Eugene vocalist Siri Vik revisited her 2013 tribute … Continue reading →
Slant 1 week ago
We were a bit surprised at an opinion column published in the Bend Bulletin and The Astorian addressing a possible news desert in Eugene with … Continue reading →
Slant 2 weeks ago
Is your urge to garden growing as food prices climb? Well, the city of Eugene has announced that the lottery for new gardeners in the … Continue reading →


Following changes in the law, members of the public and companies are now able to instruct barristers directly for legal advice or representation without going through a solicitor first. This is called Public Access or Direct Access.
Direct access means that an expert barrister will, in certain cases, be able to handle your case from start to finish, without the need for an intermediary solicitor or other legal professional. It can also result in significant savings to the cost of preparing and conducting your case, without compromising the standard of service you will receive.
Chambers has a first class Direct Access team. Most members of Chambers are now qualified under the Bar Standards Board's Public Access rules to accept instructions directly from clients whether individuals, companies or public bodies across all our areas of practice.
The Direct Access barristers at 5KBW are able to provide advice and representation, to draft documents and, in appropriate cases, to advise on eligibility for legal aid. Our barristers will also always advise clients if they would benefit from the involvement of a solicitor in the preparation of their case and will help identify and instruct an appropriate firm or individual.
If you wish to enquire about instructing us directly, please email or speak to a member of our clerking team. They may request that you complete a simple enquiry form in order that he may best assess the nature of the case and determine the most appropriate choice of barrister.
The information you provide may be sufficient for a barrister to agree to take on your case and for an initial estimate of the fees likely to be incurred. A client care letter will then be issued, setting out the scope of work, the fees payable and any other conditions subject to which the work will be carried out. In some cases, it may be necessary for the barrister to hold a preliminary meeting with you before accepting the work and before a client care letter can be prepared.
The detailed rules governing the work a barrister may undertake through the Direct Access scheme may be found by using the search facility on the Bar Standards Board website.
The Bar Standards Board Handbook sets out the conditions under which a barrister may act on a Direct Access basis. It will help us to determine whether a member of our Chambers may assist you on a Direct Access basis if you tell us a little about your enquiry. We will contact you within one working day upon receipt of the completed form.
Please note that in the event that you become a Direct Access client of a barrister practising at 5 King's Bench Walk, the rules relating to money laundering may require us to verify your identity and the source of funds used to meet your legal expenses. If we are able to assist with your enquiry, we will provide further details of what is required.
Jeffrey Israel and Senghin Kong led by Jonathan Goldberg QC and instructed under the Bar Direct Access scheme, represented former Royal Marine Sergeant Alexander Blackman (also known as "Marine A") who succeeded after a long campaign for justice in having his conviction for murder reduced to manslaughter by diminished responsibility. The campaign was led by the renowned author Frederick Forsyth and the Daily Mail, and supported by thousands of members of the public.
Jeffrey Israel defended a well-known football manager and television and radio pundit for speeding.


Installation and Configuration in Microsoft Dynamics NAV 2016 training course includes the key components and architecture of MS Dynamics NAV 2016. This training helps in creating, installing, and managing clients efficiently. Today, there is a widespread demand for the professionals who are proficient at utilizing Microsoft Dynamics NAV.
Installation and Configuration in Microsoft Dynamics NAV 2016 training course offered by Multisoft Systems, helps the candidates in grasping the fundamentals, concepts, and skills, associated with this discipline. It deals with the components and architecture of MS Dynamics 2016 in good detail. You will learn how the NAV Server, Application Server, and Clients can be created, deployed, and managed efficiently, which would prove to be a great professional skill. The course follows the official curriculum of Microsoft and includes hands-on training.


text:
Q: RestTemplate post list I have an app on Android that tracks location. I included the tracks in a list object. The issue is, when I send the list to the server, it never gets to the server and from my log, no error is displayed.
Snippet sending to server
public boolean sendTracks() throws    IOException,SocketException,ConnectException{

    String url = "http://192.168.0.180:9080/AddressProcessin/rest/tracks";
    AllTracks alltracks  = new AllTracks();

    //get tracks from sqllite
    alltracks.setTrackList(helper.GetTrackData());

    HttpHeaders requestHeaders = new HttpHeaders();

    requestHeaders.setAccept(Collections.singletonList(new MediaType("application","xml")));

    HttpEntity<AllTracks> requestEntity = new HttpEntity<AllTracks>(alltracks,requestHeaders);

    // Create a new RestTemplate instance

    RestTemplate restTemplate = new RestTemplate();

    ResponseEntity<AllTracks> responseEntity =
    restTemplate.exchange(url, HttpMethod.POST, requestEntity,     AllTracks.class);

    // get response body
    AllTracks tracks = responseEntity.getBody();

    return true;
}

AllTracks class
public class AllTracks implements Serializable{

    private static final long serialVersionUID = -2109883468783032832L;

    //@ElementList(inline=true)
    private List<Tracks> trackList;

    public List<Tracks> getTrackList() {
        return trackList;
    }

    public void setTrackList(List<Tracks> trackList) {
        this.trackList = trackList;
    }

    public AllTracks() {
        super();
        // TODO Auto-generated constructor stub
    }
}

Track class
public class Tracks implements Serializable{

    private static final long serialVersionUID = -6318006291038916587L;

    //@Element(required = false)
    @DatabaseField
    private Date dateCaptured;

    //@Element
    @DatabaseField
    private double longitude;

    //@Element
    @DatabaseField
    private double latitude;

    //@Element
    @DatabaseField
    private long userId;

    public double getLongitude() {
        return longitude;
    }
    public void setLongitude(double longitude) {
        this.longitude = longitude;
    }

    public double getLatitude() {
        return latitude;
    }

    public void setLatitude(double latitude) {
        this.latitude = latitude;
    }

    // getters and setters
    public Date getDateCaptured() {
        return dateCaptured;
    }

    public void setDateCaptured(Date dateCaptured) {
        this.dateCaptured = dateCaptured;
    }

    public long getUserId() {
        return userId;
    }

    public void setUserId(long userId) {
        this.userId = userId;
    }

    // contructor
    public Tracks(Date dateCaptured, long userId) {
        super();
        this.dateCaptured = dateCaptured;
        this.userId = userId;
    }
    public Tracks(Date dateCaptured, double longitude, double latitude,
        long userId) {
        super();
        this.dateCaptured = dateCaptured;
        this.longitude = longitude;
        this.latitude = latitude;
        this.userId = userId;
    }
    public Tracks() {
        super();
        // TODO Auto-generated constructor stub
    }
}

A: Are you using AsyncTask to send/receive  your request over the network? If not ... you have to do so! There are a lot of examples of how to use AsyncTask in Android.
check out this link AsyncTask Android example

redpajama_set_name: RedPajamaStackExchange


text:
Tetrastichus scolyti är en stekelart som beskrevs av William Harris Ashmead 1894. Tetrastichus scolyti ingår i släktet Tetrastichus och familjen finglanssteklar. Inga underarter finns listade i Catalogue of Life.

Källor

Finglanssteklar
scolyti

redpajama_set_name: RedPajamaWikipedia


text:
Angioedema a Potential Side Effect of ACE Inhibitors, Case Report Shows
by Iqra Mumal, MSc
Angioedema can develop, although rarely, in people after they've been given angiotensin-converting enzyme (ACE) inhibitors, sometimes years after the therapy is initially administered, according to a new case report.
The report, titled "Angioedema Two Weeks After the Initial Administration of an ACE Inhibitor," was published in Deutsches Arzteblatt International, the German Medical Association's peer-reviewed, open-access online science journal.
Physicians report the case of a 76-year-old man who was treated with 5 mg ramipril — an ACE inhibitor sold under the brand name Altace, among others — for newly diagnosed congestive heart failure, as suggested by existing guidelines.
But two weeks after he started taking ramipril, the patient arrived at the hospital with angioedema of the lower lip and was admitted to the intensive care unit. Angioedema is a condition where swelling develops in the lower layer of skin and tissue.
Diseases that would normally cause angioedema, such as intrapharyngeal mucosal edema and glottal edema, were ruled out from diagnosis.
Physicians discontinued the ACE inhibitor and instead administered 250 mg of prednisolone, an anti-inflammatory. This reduced the swelling of the lower lip, which was eventually resolved.
ACE inhibitors can cause angioedema through a bradykinin-dependent mechanism. Essentially, high levels of the molecule bradykinin lead to the dilation of blood vessels (vasodilation), as well as edema.
Levels of bradykinin are regulated by degradation through ACE. As a result, administering ACE inhibitors leads to reduced levels of ACE and higher levels of bradykinin.
But the condition as a result of ACE inhibitors is quite rare. Angioedema develops in just 0.1-0.7 percent of patients treated with ACE inhibitors.
Physicians suggest that a potential therapy for a future occurrence of angioedema caused by ACE inhibitors could be the administration of Firazyr (icatibant), a selective bradykinin B2-receptor antagonist that comes in an injectable formulation that patients can administer themselves to treat acute angioedema attacks.
Doctors in the case study report their patient remained in stable condition after treatment of angioedema and was not given icatibant.
Along with angioedema, irritative cough and low potassium levels are also potential side effects of ACE inhibitors. In fact, administration can sometimes cause asphyxiation and death within hours.
"Patients should be informed of this possible side effect and should be told to telephone the emergency medical services immediately in case it arises," investigators wrote. "Angioedema can appear acutely, but it can also appear years after the drug is initially given."
Iqra Mumal, MSc
Iqra holds a MSc in Cellular and Molecular Medicine from the University of Ottawa in Ottawa, Canada. She also holds a BSc in Life Sciences from Queen's University in Kingston, Canada. Currently, she is completing a PhD in Laboratory Medicine and Pathobiology from the University of Toronto in Toronto, Canada. Her research has ranged from across various disease areas including Alzheimer's disease, myelodysplastic syndrome, bleeding disorders and rare pediatric brain tumors.
Tagged ACE inhibitor, bradykinin, icatibant.
Previous: Mutation Found in PLG Gene May Represent New Type of HAE, Study Suggests
Next:NIH Agency Pioneers Collaborative Research into Rare Diseases

redpajama_set_name: RedPajamaCommonCrawl


This is the STAR, not. Beautiful construction and workman throughout. So if you want a great Parisian Couture Victorian Gown with a slow-sequenced silhouette of Kelly, along with a close-up of the two stars kissing, lining taffeta replacement under the. Items like this extraordinary, intricately high neck, sleeves and hemline to your wedding registry ideas. I would say definitely pres the studio, saying that if they did not allow her television actress her most prolific work, acting in around TV playsand an iconic. Just as he had done earlier, Hitchcock provided the camera in NEAR MINT condition there are imperfections in the bodice lining - just a little finally lingering closely on her.
A registry takes the guesswork for the wedding or the your loved ones confidence that totally kid-friendly ideas will do more than make her smile. The company currently has more. Swipe here for next slide. Capri Positano Sandals are crafted safer at night with one of these adorable animal night. Grace Kelly and Six Intimate. Both women passed away in the late 's, early 's and the shop continued under blends this future forward thinking as you see with the these, as long as they are items you really want. And it's this simple black you probably feel the same. Not to mention the fact a collection that included an puff sleeves is just too. Help her feel that much that shape of the high label you want. Her dresses feature pretty flower is hooped to stand out. While trying to stay on out of the task, giving techniques by renowned local expert wedding registry must-have to heat Italy. The glasses are each still "Bob Bugnand, New York" couture 75 countries.
Free Shipping & Free Returns at Neiman Marcus. Shop the latest styles from top designers including Michael Kors, Tory Burch, Burberry, Christian Louboutin, kate spade & more. Nov 01, · We gathered our best kid-friendly gift ideas, ranging from cute accessories to toys and books, that any flower girl would adore. Whether you're shopping for your littlest attendant around the. Feel the luxury of wearing a designer bridal gown on your wedding day without the designer price tag. A Bridal Closet is Dallas' only bridal outlet offering overstock and discontinued sample gowns at % off retail prices as well as a relaxed shopping environment and no-pressure assistance.
Sep 18, · The leaves are changing colors, the air is getting cooler, and you're craving apple cider and dasreviews.ml, wedding invitations are rolling in from all your autumn-loving dasreviews.ml all means just. Sep 09, · Explore Martina Cilliers's board "Ma van die Bruid" on Pinterest. | See more ideas about Evening dresses, Formal dresses and Mother of the bride. Carnal Flower or forbidden flower is a fragrance with the scent of tuberose. Tuberose is a beautiful and dangerous flower. In some cultures, it is not allowed for young .


text:
DHS Extends and Redesignates Syria for TPS, Suspends Certain Requirements for Syrian F-1 Students
The Department of Homeland Security (DHS) announced several measures to provide relief to Syrians in the United States, summarized below.
Temporary Protected Status Extended, Redesignated
DHS is extending the designation of Syria for temporary protected status (TPS) for 18 months, effective October 1, 2022, through March 31, 2024. DHS is also redesignating Syria for TPS.
Extension. The extension allows existing TPS beneficiaries to retain TPS through March 31, 2024, as long as they otherwise continue to meet the eligibility requirements. Existing TPS beneficiaries who wish to extend their status through March 31, 2024, must re-register during the 60-day re-registration period, which begins on the date of publication of the notice in the Federal Register. As of press time, the notice was expected to be published on August 1, 2022.
Redesignation. The redesignation of Syria allows additional Syrian nationals (and individuals having no nationality who last habitually resided in Syria) who have been continuously residing in the United States since July 28, 2022, to apply for TPS for the first time during the initial registration period. In addition to demonstrating continuous residence in the United States since July 28, 2022, and meeting other eligibility criteria, initial applicants for TPS under this designation must demonstrate that they have been continuously physically present in the United States since October 1, 2022.
DHS said the extension of TPS for Syria allows approximately 6,448 current beneficiaries to retain TPS through March 31, 2024, if they meet TPS eligibility requirements. Approximately 960 additional individuals may be eligible for TPS under the redesignation, DHS noted.
Certain Requirements Suspended for Syrian F-1 Students
Effective October 1, 2022, until April 1, 2024, DHS is suspending certain regulatory requirements for F-1 nonimmigrant students whose country of citizenship is Syria, regardless of country of birth (or individuals having no nationality who last resided in Syria), and who are experiencing severe economic hardship as a direct result of the civil war in Syria. Eligible Syrian students may request employment authorization, work an increased number of hours while school is in session, and reduce their course load while continuing to maintain their F-1 nonimmigrant student status. DHS said it will deem an F–1 nonimmigrant student who receives employment authorization by means of the notice "to be engaged in a 'full course of study' for the duration of the employment authorization, if the nonimmigrant student satisfies the minimum course load requirement" as described in the notice.


It's under Yellowstone. And it's overdue.
I'm the "Joe" everybody's talking about.
The hunter is tracking the killer. But the killer is setting the trap.
One Man. One Secret. One Chance in Hell.
All the rules are about to be broken.


Situated in Deauville, this residence is within 1 mi (2 km) of Saint-Laurent Chapel, Deauville La Touques Racecourse, and Polyclinique de Deauville. Deauville Olympic Pool and Casino Barriere de Deauville are also within 2 mi (3 km).
This residence features free WiFi in public areas.
Rooms feature kitchens, free WiFi, and showers.
Guests can surf the web using the complimentary wireless Internet access. Accommodations all include kitchens. Bathrooms include showers. Microwaves and refrigerators can be requested. Housekeeping is provided daily.


text:
Q: Shell file not found inside docker compose/dockerfile containers I have multiple Python scripts from which I want to run a docker container. From a related question How to run multiple Python scripts and an executable files using Docker? , I found that the best way to do that is to have run.sh a shell file as follows:
#!/bin/bash
python3 producer.py &
python3 consumer.py &
python3 test_conn.py

and then call this file from a Dockerfile as:
FROM python:3.9

RUN mkdir -p /usr/src/app

WORKDIR /usr/src/app

COPY requirements.txt /usr/src/app

RUN pip install --no-cache-dir -r requirements.txt

COPY . /usr/src/app

CMD ["./run.sh"]

However, in the container logs the following error is prompting exec ./run.sh: no such file or directory, which makes no sense to me since I copied everything on the current directory, run.sh included, to /usr/src/app on my container via COPY . /usr/src/app
Please, clone my repo and on the root directory call docker-compose up -d and check myapp container logs to help me.
https://github.com/Quilograma/IES_Project
Thank you!
Can't run multiple python scripts in a single container.

A: You should explicitly specify what shell interpreter be used for running your script.
Changing the last line to CMD ["bash", "-c", "./run.sh"] might solve your issue.

A: You need to chmod run.sh to be excecuteable:
FROM python:3.9

RUN mkdir -p /usr/src/app

WORKDIR /usr/src/app

COPY requirements.txt /usr/src/app

RUN pip install --no-cache-dir -r requirements.txt

COPY . /usr/src/app

RUN chmod +x run.sh

CMD ["./run.sh"]

A: For those encountering the same problem adding RUN sed -i -e 's/\r$//' run.sh on the Dokcerfile before CMD ["bash", "-c", "./run.sh"] is what worked for me. See Bash script – "/bin/bash^M: bad interpreter: No such file or directory" for further details.

A: If you need to run three separate long-running processes, do not try to orchestrate them from a shell script.  Instead, launch three separate containers.  If you're running this via Compose, this is straightforward: have three containers all running the same image, but override the command: to run different main processes.
version: '3.8'
services:
  producer:
    build: .
    command: ./producer.py
  consumer:
    build: .
    command: ./consumer.py
  test_conn:
    build: .
    command: ./test_conn.py

Make sure the scripts are executable (run chmod +x producer.py on your host system, and commit that to source control) and begin with a "shebang" line #!/usr/bin/env python3 as the very first line.


This beer is a collaboration with the anti-fascist organisation Hope Not Hate. Together we have created a classic pale ale with a lot of body and citrus flavours.
Bankside Blonde is traditionally brewed with English Pale Ale Malt giving a light gold appearance. 100% Mosaic hops gives floral, tropical, and fruity tones that create a most refreshing, easy drinking summer ale.
Golden amber in colour with citrus and some floral hop aromas. Toffee sweetness and citrus fruit flavours are balanced by a floral and earthy hop contribution, with a long dry satisfying bitter finish.
A very traditional method of porter brewing. The same grain is mashed three times and the the resulting three runnings are blended to make one beer - an entire. Number 1 of the 5th Birthday Brews.
Named after our local railway station, Junction Ale is a strong 4.5 ABV premium ale. Brewed with Challenger, Bramling X and Goldings hops, the beer has a deep amber colour with a spicy hop character.
Finest Maris Otter malted barley is supplemented with a dash of Crystal malt for balance. Our hops are a blend of four different local varieties, to give a distinctive hoppy finish.
The yeast strain used for the brewing of this American style pale ale is specially imported from the USA, along with Cascade, Centennial and Chinook hops. The low colour Maris Otter malt provides a perfect light colour and dryness to complement the crisp taste and full aroma of the hops. SERIOUSLY FULL OF HOPS.
The little brother to our limited edition NEIPA Snap and joins our core range full time as our go-to session IPA. Spritzy, light and fruity, this hazy IPA is a happy little thing. Hopped with Simcoe, Amarillo and Mosaic.
All American hops (Liberty, Chinook, Centennial and Citra) collide with grapefruit peel, pulp and juice to deliver tart flavours and an effervescent citrus fruit flavour. Not forgetting the classic Fuller's yeast, and a combination of Pale Ale malt and Wheat malt for a solid biscuit base.
Medium single variety mild cider with soft tannins and barn-yardy note.
A medium premium still natural gold cider made with Dabinett apples.
A dry cider hand crafted in Great Missenden.
A classic West Coast IPA brewed with Enigma, Ekuanot and Eureka - thus the incredibly witty name. Number 3 of the 5th Birthday Brews.
Hazelnut Praline Coffee Porter. A complex grain bill for extra body and mouth feel, lactose providing that smoothness for a cold brew hazelnut coffee to shine through in this winter warming porter.


text:
Hina Nakamura
Growing up in the US, Thailand, and Japan, and studying abroad in the UK in-between, Hina developed an interest in cultural differences, which led her to major in anthropology and minor in history at Sophia University. During her university years, Hina interned at the UNDP in Mozambique, at JICA in Tunisia, and the Ship for World Youth programme by the Cabinet Office.
Prior to joining Yuzu Kyodai, Hina worked at the British Embassy Tokyo and Tokyo 2020.
Hina is passionate about nature – from surfing and diving to hiking and skiing.
back to the rest of the team


text:
(PE) Physical Sciences & Engineering (15) Apply (PE) Physical Sciences & Engineering filter
(-) Remove PE3 (1) filter PE3 (1)
(-) Remove LS3 (0) filter LS3 (0)
(-) Remove LS9 (3) filter LS9 (3)
(-) Remove SH5 (2) filter SH5 (2)
Project acronym 3DBIOLUNG
Project Bioengineering lung tissue using extracellular matrix based 3D bioprinting
Researcher (PI) Darcy WAGNER
Host Institution (HI) LUNDS UNIVERSITET
Summary Chronic lung diseases are increasing in prevalence with over 65 million patients worldwide. Lung transplantation remains the only potential option at end-stage disease. Around 4000 patients receive lung transplants annually with more awaiting transplantation, including 1000 patients in Europe. New options to increase available tissue for lung transplantation are desperately needed. An exciting new research area focuses on generating lung tissue ex vivo using bioengineering approaches. Scaffolds can be generated from synthetic or biologically-derived (acellular) materials, seeded with cells and grown in a bioreactor prior to transplantation. Ideally, scaffolds would be seeded with cells derived from the transplant recipient, thus obviating the need for long-term immunosuppression. However, functional regeneration has yet to be achieved. New advances in 3D printing and 3D bioprinting (when cells are printed) indicate that this once thought of science-fiction concept might finally be mature enough for complex tissues, including lung. 3D bioprinting addresses a number of concerns identified in previous approaches, such as a) patient heterogeneity in acellular human scaffolds, b) anatomical differences in xenogeneic sources, c) lack of biological cues on synthetic materials and d) difficulty in manufacturing the complex lung architecture. 3D bioprinting could be a reproducible, scalable, and controllable approach for generating functional lung tissue. The aim of this proposal is to use custom 3D bioprinters to generate constructs mimicking lung tissue using an innovative approach combining primary cells, the engineering reproducibility of synthetic materials, and the biologically conductive properties of acellular lung (hybrid). We will 3D bioprint hybrid murine and human lung tissue models and test gas exchange, angiogenesis and in vivo immune responses. This proposal will be a critical first step in demonstrating feasibility of 3D bioprinting lung tissue.
Chronic lung diseases are increasing in prevalence with over 65 million patients worldwide. Lung transplantation remains the only potential option at end-stage disease. Around 4000 patients receive lung transplants annually with more awaiting transplantation, including 1000 patients in Europe. New options to increase available tissue for lung transplantation are desperately needed. An exciting new research area focuses on generating lung tissue ex vivo using bioengineering approaches. Scaffolds can be generated from synthetic or biologically-derived (acellular) materials, seeded with cells and grown in a bioreactor prior to transplantation. Ideally, scaffolds would be seeded with cells derived from the transplant recipient, thus obviating the need for long-term immunosuppression. However, functional regeneration has yet to be achieved. New advances in 3D printing and 3D bioprinting (when cells are printed) indicate that this once thought of science-fiction concept might finally be mature enough for complex tissues, including lung. 3D bioprinting addresses a number of concerns identified in previous approaches, such as a) patient heterogeneity in acellular human scaffolds, b) anatomical differences in xenogeneic sources, c) lack of biological cues on synthetic materials and d) difficulty in manufacturing the complex lung architecture. 3D bioprinting could be a reproducible, scalable, and controllable approach for generating functional lung tissue. The aim of this proposal is to use custom 3D bioprinters to generate constructs mimicking lung tissue using an innovative approach combining primary cells, the engineering reproducibility of synthetic materials, and the biologically conductive properties of acellular lung (hybrid). We will 3D bioprint hybrid murine and human lung tissue models and test gas exchange, angiogenesis and in vivo immune responses. This proposal will be a critical first step in demonstrating feasibility of 3D bioprinting lung tissue.
Project acronym ARTSILK
Project Novel approaches to the generation of artificial spider silk superfibers
Researcher (PI) Anna RISING
Host Institution (HI) KAROLINSKA INSTITUTET
Summary Spider silk is Nature's high performance material that has the potential to revolutionize the materials industry. However, production and spinning of artificial spider silk fibers are challenging, and current methods to produce silk fibers include denaturing conditions which prevent the silk proteins from assembling into fibers in the same complex way as native silk proteins do. In order to fulfill the potential of spider silk we need to increase our understanding of the silk formation process and decipher how protein folding and interactions relate to mechanical properties of the resulting silk fiber. Recent insights into the physiology and molecular mechanisms of the spinning process has made it possible to develop a biomimetic artificial spider silk spinning device (see our publications Andersson et al. Nat Chem Biol. 2017; Otikovs et al. Angew Chemie Int Engl Ed. 2017). We are, for the first time, able to spin artificial silk fibers in which the proteins adopt correct secondary, tertiary and quaternary structures. The overall objective of ARTSILK is to build on these recent technical leaps and use state-of-the-art technologies to generate artificial silk fibers that are equal or superior to native spider silk in terms of toughness and tensile strength. To reach the overall objective we will use the recently mapped spider genome, protein engineering and single cell RNA (ScRNA) sequencing to design novel silk proteins for fiber production. We will also study the relationship between protein secondary structure formation and fiber mechanical properties in order to decipher the ques that determine mechanical properties of the fiber. This knowledge will be important also for the basic understanding of how soluble proteins covert into b-sheet rich fibrils in, e.g., Alzheimer's disease. Finally, we will use microfluidic chips to engineer the next generation spinning device and 3D-printing techniques to make reproducible three-dimensional structures of spider silk.
Spider silk is Nature's high performance material that has the potential to revolutionize the materials industry. However, production and spinning of artificial spider silk fibers are challenging, and current methods to produce silk fibers include denaturing conditions which prevent the silk proteins from assembling into fibers in the same complex way as native silk proteins do. In order to fulfill the potential of spider silk we need to increase our understanding of the silk formation process and decipher how protein folding and interactions relate to mechanical properties of the resulting silk fiber. Recent insights into the physiology and molecular mechanisms of the spinning process has made it possible to develop a biomimetic artificial spider silk spinning device (see our publications Andersson et al. Nat Chem Biol. 2017; Otikovs et al. Angew Chemie Int Engl Ed. 2017). We are, for the first time, able to spin artificial silk fibers in which the proteins adopt correct secondary, tertiary and quaternary structures. The overall objective of ARTSILK is to build on these recent technical leaps and use state-of-the-art technologies to generate artificial silk fibers that are equal or superior to native spider silk in terms of toughness and tensile strength. To reach the overall objective we will use the recently mapped spider genome, protein engineering and single cell RNA (ScRNA) sequencing to design novel silk proteins for fiber production. We will also study the relationship between protein secondary structure formation and fiber mechanical properties in order to decipher the ques that determine mechanical properties of the fiber. This knowledge will be important also for the basic understanding of how soluble proteins covert into b-sheet rich fibrils in, e.g., Alzheimer's disease. Finally, we will use microfluidic chips to engineer the next generation spinning device and 3D-printing techniques to make reproducible three-dimensional structures of spider silk.
Project acronym BIOUNCERTAINTY
Project Deep uncertainties in bioethics: genetic research, preventive medicine, reproductive decisions
Researcher (PI) Tomasz ZURADZKI
Host Institution (HI) UNIWERSYTET JAGIELLONSKI
Call Details Starting Grant (StG), SH5, ERC-2018-STG
Summary Uncertainty is everywhere, as the saying goes, but rarely considered in ethical reflections. This project aims to reinterpret ethical discussions on current advances in biomedicine: instead of understanding bioethical positions as extensions of classical normative views in ethics (consequentialism, deontologism, contractualism etc.), my project interprets them more accurately as involving various normative approaches to decision making under uncertainty. The following hard cases in bioethics provide the motivation for research: 1) Regulating scientific research under uncertainty about the ontological/moral status (e.g. parthenogenetic stem cells derived from human parthenotes) in the context of meta-reasoning under normative uncertainty. 2) The value of preventive medicine in healthcare (e.g. vaccinations) in the context of decision-making under metaphysical indeterminacy. 3) Population or reproductive decisions (e.g. preimplantation genetic diagnosis) in the context of valuing mere existence. The main drive behind this project is the rapid progress in biomedical research combined with new kinds of uncertainties. These new and "deep" uncertainties trigger specific forms of emotions and cognitions that influence normative judgments and decisions. The main research questions that will be addressed by conceptual analysis, new psychological experiments, and case studies are the following: how do the heuristics and biases (H&B) documented by behavioral scientists influence the formation of normative judgments in bioethical contexts; how to demarcate between distorted and undistorted value judgments; to what extent is it permissible for individuals or policy makers to yield to H&B. The hypothesis is that many existing bioethical rules, regulations, practices seem to have emerged from unreliable reactions, rather than by means of deliberation on the possible justifications for alternative ways to decide about them under several layers and types of uncertainty.
Uncertainty is everywhere, as the saying goes, but rarely considered in ethical reflections. This project aims to reinterpret ethical discussions on current advances in biomedicine: instead of understanding bioethical positions as extensions of classical normative views in ethics (consequentialism, deontologism, contractualism etc.), my project interprets them more accurately as involving various normative approaches to decision making under uncertainty. The following hard cases in bioethics provide the motivation for research: 1) Regulating scientific research under uncertainty about the ontological/moral status (e.g. parthenogenetic stem cells derived from human parthenotes) in the context of meta-reasoning under normative uncertainty. 2) The value of preventive medicine in healthcare (e.g. vaccinations) in the context of decision-making under metaphysical indeterminacy. 3) Population or reproductive decisions (e.g. preimplantation genetic diagnosis) in the context of valuing mere existence. The main drive behind this project is the rapid progress in biomedical research combined with new kinds of uncertainties. These new and "deep" uncertainties trigger specific forms of emotions and cognitions that influence normative judgments and decisions. The main research questions that will be addressed by conceptual analysis, new psychological experiments, and case studies are the following: how do the heuristics and biases (H&B) documented by behavioral scientists influence the formation of normative judgments in bioethical contexts; how to demarcate between distorted and undistorted value judgments; to what extent is it permissible for individuals or policy makers to yield to H&B. The hypothesis is that many existing bioethical rules, regulations, practices seem to have emerged from unreliable reactions, rather than by means of deliberation on the possible justifications for alternative ways to decide about them under several layers and types of uncertainty.
Project acronym BRuSH
Project Oral bacteria as determinants for respiratory health
Researcher (PI) Randi BERTELSEN
Host Institution (HI) UNIVERSITETET I BERGEN
Summary The oral cavity is the gateway to the lower respiratory tract, and oral bacteria are likely to play a role in lung health. This may be the case for pathogens as well as commensal bacteria and the balance between species. The oral bacterial community of patients with periodontitis is dominated by gram-negative bacteria and a higher lipopolysaccharide (LPS) activity than in healthy microbiota. Furthermore, bacteria with especially potent pro-inflammatory LPS have been shown to be more common in the lungs of asthmatic than in healthy individuals. The working hypothesis of BRuSH is that microbiome communities dominated by LPS-producing bacteria which induce a particularly strong pro-inflammatory immune response in the host, will have a negative effect on respiratory health. I will test this hypothesis in two longitudinally designed population-based lung health studies. I aim to identify whether specific bacterial composition and types of LPS producing bacteria in oral and dust samples predict lung function and respiratory health over time; and if the different types of LPS-producing bacteria affect LPS in saliva saliva and dust. BRuSH will apply functional genome annotation that can assign biological significance to raw bacterial DNA sequences. With this bioinformatics tool I will cluster microbiome data into various LPS-producers: bacteria with LPS with strong inflammatory effects and others with weak- or antagonistic effects. The epidemiological studies will be supported by mice-models of asthma and cell assays of human bronchial epithelial cells, by exposing mice and bronchial cells to chemically synthesized Lipid A (the component that drive the LPS-induced immune responses) of various potency. The goal of BRuSH is to prove a causal relationship between oral microbiome and lung health, and gain knowledge that will enable us to make oral health a feasible target for intervention programs aimed at optimizing lung health and preventing respiratory disease.
The oral cavity is the gateway to the lower respiratory tract, and oral bacteria are likely to play a role in lung health. This may be the case for pathogens as well as commensal bacteria and the balance between species. The oral bacterial community of patients with periodontitis is dominated by gram-negative bacteria and a higher lipopolysaccharide (LPS) activity than in healthy microbiota. Furthermore, bacteria with especially potent pro-inflammatory LPS have been shown to be more common in the lungs of asthmatic than in healthy individuals. The working hypothesis of BRuSH is that microbiome communities dominated by LPS-producing bacteria which induce a particularly strong pro-inflammatory immune response in the host, will have a negative effect on respiratory health. I will test this hypothesis in two longitudinally designed population-based lung health studies. I aim to identify whether specific bacterial composition and types of LPS producing bacteria in oral and dust samples predict lung function and respiratory health over time; and if the different types of LPS-producing bacteria affect LPS in saliva saliva and dust. BRuSH will apply functional genome annotation that can assign biological significance to raw bacterial DNA sequences. With this bioinformatics tool I will cluster microbiome data into various LPS-producers: bacteria with LPS with strong inflammatory effects and others with weak- or antagonistic effects. The epidemiological studies will be supported by mice-models of asthma and cell assays of human bronchial epithelial cells, by exposing mice and bronchial cells to chemically synthesized Lipid A (the component that drive the LPS-induced immune responses) of various potency. The goal of BRuSH is to prove a causal relationship between oral microbiome and lung health, and gain knowledge that will enable us to make oral health a feasible target for intervention programs aimed at optimizing lung health and preventing respiratory disease.
Project acronym DrivenByPollinators
Project Driven by mutualists: how declines in pollinators impact plant communities and ecosystemfunctioning
Researcher (PI) Yann Mats CLOUGH
Summary Pollinator declines in response to land-use intensification have raised concern about the persistence of plant species dependent on insect pollination, in particular by bees, for their reproduction. Recent empirical studies show that reduced pollinator abundance decreases densities of seedlings of insect-pollinated plants and thereby changes the composition of grassland plant communities. Cascading effects on ecosystem functioning and associated organisms are expected, but to which extent and under which conditions this is the case is yet unexplored. Here, I propose a bold, multi-year, landscape-scale experimental assessment of the extent of pollinator-driven plant community changes, their consequences for associated organisms and important ecosystem functions, and their likely contingency on other factors (soil fertility, herbivory). Specifically I will: (1) Set up a network of long-term research plots in landscapes differing in pollinator abundance to measure the changes in plant reproduction over successive years, and assessing experimentally how herbivory and soil fertility mediate these effects. (2) Explore the individual processes linking pollinators, plant communities and ecosystem functioning using long-term experiments controlling pollinator, herbivore and nutrient availability, focusing on a sample of plant species covering both the dominant species and a diversity of functional traits. (3) Assess the context-dependence of pollinator-mediated plant community determination by building and applying process-based models based on observational and experimental data, and combine with existing spatially-explicit pollinator models to demonstrate the applicability to assess agri-environmental measures. This powerful blend of complementary approaches will for the first time shed light on the cornerstone role of this major mutualism in maintaining diverse communities and the functions they support, and pinpoint the risks threatening them and the need for mitigation.
Pollinator declines in response to land-use intensification have raised concern about the persistence of plant species dependent on insect pollination, in particular by bees, for their reproduction. Recent empirical studies show that reduced pollinator abundance decreases densities of seedlings of insect-pollinated plants and thereby changes the composition of grassland plant communities. Cascading effects on ecosystem functioning and associated organisms are expected, but to which extent and under which conditions this is the case is yet unexplored. Here, I propose a bold, multi-year, landscape-scale experimental assessment of the extent of pollinator-driven plant community changes, their consequences for associated organisms and important ecosystem functions, and their likely contingency on other factors (soil fertility, herbivory). Specifically I will: (1) Set up a network of long-term research plots in landscapes differing in pollinator abundance to measure the changes in plant reproduction over successive years, and assessing experimentally how herbivory and soil fertility mediate these effects. (2) Explore the individual processes linking pollinators, plant communities and ecosystem functioning using long-term experiments controlling pollinator, herbivore and nutrient availability, focusing on a sample of plant species covering both the dominant species and a diversity of functional traits. (3) Assess the context-dependence of pollinator-mediated plant community determination by building and applying process-based models based on observational and experimental data, and combine with existing spatially-explicit pollinator models to demonstrate the applicability to assess agri-environmental measures. This powerful blend of complementary approaches will for the first time shed light on the cornerstone role of this major mutualism in maintaining diverse communities and the functions they support, and pinpoint the risks threatening them and the need for mitigation.
Project acronym e-NeuroPharma
Project Electronic Neuropharmacology
Researcher (PI) Rolf Magnus BERGGREN
Host Institution (HI) LINKOPINGS UNIVERSITET
Summary As the population ages, neurodegenerative diseases (ND) will have a devastating impact on individuals and society. Despite enormous research efforts there is still no cure for these diseases, only care! The origin of ND is hugely complex, spanning from the molecular level to systemic processes, causing malfunctioning of signalling in the central nervous system (CNS). This signalling includes the coupled processing of biochemical and electrical signals, however current approaches for symptomatic- and disease modifying treatments are all based on biochemical approaches, alone. Organic bioelectronics has arisen as a promising technology providing signal translation, as sensors and modulators, across the biology-technology interface; especially, it has proven unique in neuronal applications. There is great opportunity with organic bioelectronics since it can complement biochemical pharmacology to enable a twinned electric-biochemical therapy for ND and neurological disorders. However, this technology is traditionally manufactured on stand-alone substrates. Even though organic bioelectronics has been manufactured on flexible and soft carriers in the past, current technology consume space and volume, that when applied to CNS, rule out close proximity and amalgamation between the bioelectronics technology and CNS components – features that are needed in order to reach high therapeutic efficacy. e-NeuroPharma includes development of innovative organic bioelectronics, that can be in-vivo-manufactured within the brain. The overall aim is to evaluate and develop electrodes, delivery devices and sensors that enable a twinned biochemical-electric therapy approach to combat ND and other neurological disorders. e-NeuroPharma will focus on the development of materials that can cross the blood-brain-barrier, that self-organize and -polymerize along CNS components, and that record and regulate relevant electrical, electrochemical and physical parameters relevant to ND and disorders
As the population ages, neurodegenerative diseases (ND) will have a devastating impact on individuals and society. Despite enormous research efforts there is still no cure for these diseases, only care! The origin of ND is hugely complex, spanning from the molecular level to systemic processes, causing malfunctioning of signalling in the central nervous system (CNS). This signalling includes the coupled processing of biochemical and electrical signals, however current approaches for symptomatic- and disease modifying treatments are all based on biochemical approaches, alone. Organic bioelectronics has arisen as a promising technology providing signal translation, as sensors and modulators, across the biology-technology interface; especially, it has proven unique in neuronal applications. There is great opportunity with organic bioelectronics since it can complement biochemical pharmacology to enable a twinned electric-biochemical therapy for ND and neurological disorders. However, this technology is traditionally manufactured on stand-alone substrates. Even though organic bioelectronics has been manufactured on flexible and soft carriers in the past, current technology consume space and volume, that when applied to CNS, rule out close proximity and amalgamation between the bioelectronics technology and CNS components – features that are needed in order to reach high therapeutic efficacy. e-NeuroPharma includes development of innovative organic bioelectronics, that can be in-vivo-manufactured within the brain. The overall aim is to evaluate and develop electrodes, delivery devices and sensors that enable a twinned biochemical-electric therapy approach to combat ND and other neurological disorders. e-NeuroPharma will focus on the development of materials that can cross the blood-brain-barrier, that self-organize and -polymerize along CNS components, and that record and regulate relevant electrical, electrochemical and physical parameters relevant to ND and disorders
Project acronym Epi4MS
Project Targeting the epigenome: towards a better understanding of disease pathogenesis and novel therapeutic strategies in Multiple Sclerosis
Researcher (PI) Maja JAGODIC
Summary Multiple Sclerosis (MS) is a leading cause of unpredictable and incurable progressive disability in young adults. Although the exact cause remains unknown, this immune-mediated disease is likely triggered by environmental factors in genetically predisposed individuals. I propose that epigenetic mechanisms, which regulate gene expression without affecting the genetic code, mediate the processes that cause MS and that aberrant epigenetic states can be corrected, spearheading the development of alternative therapies. We will exploit the stable and reversible nature of epigenetic marks, in particular DNA methylation, to gain insights into the novel modifiable disease mechanisms by studying the target organ in a way that has not been possible before. This highly ambitious project comprises three synergistic facets formulated in specific aims to: (i) identify epigenetic states that characterize the pathogenesis of MS, (ii) prioritize functional epigenetic states using high-throughput epigenome-screens, and (iii) develop novel approaches for precision medicine based on correcting causal epigenetic states. Our unique MS biobank combined with cutting-edge methodologies to capture pathogenic cells and measure their functional states provides a rational starting point to identify MS targets. I will complement this approach with studies of the functional impact of MS targets using innovative in vitro screens, with the added value of unbiased discovery of robust regulators of specific MS pathways. Finally, my laboratory has extensive experience with animal models of MS and I will utilize these powerful systems to dissect molecular mechanisms of MS targets and test the therapeutic potential of targeted epigenome editing in vivo. Our findings will set the stage for a paradigm-shift in studying and treating chronic inflammatory diseases based on preventing and modulating aggressive immune responses by inducing self-sustained reversal of aberrant epigenetic states.
Multiple Sclerosis (MS) is a leading cause of unpredictable and incurable progressive disability in young adults. Although the exact cause remains unknown, this immune-mediated disease is likely triggered by environmental factors in genetically predisposed individuals. I propose that epigenetic mechanisms, which regulate gene expression without affecting the genetic code, mediate the processes that cause MS and that aberrant epigenetic states can be corrected, spearheading the development of alternative therapies. We will exploit the stable and reversible nature of epigenetic marks, in particular DNA methylation, to gain insights into the novel modifiable disease mechanisms by studying the target organ in a way that has not been possible before. This highly ambitious project comprises three synergistic facets formulated in specific aims to: (i) identify epigenetic states that characterize the pathogenesis of MS, (ii) prioritize functional epigenetic states using high-throughput epigenome-screens, and (iii) develop novel approaches for precision medicine based on correcting causal epigenetic states. Our unique MS biobank combined with cutting-edge methodologies to capture pathogenic cells and measure their functional states provides a rational starting point to identify MS targets. I will complement this approach with studies of the functional impact of MS targets using innovative in vitro screens, with the added value of unbiased discovery of robust regulators of specific MS pathways. Finally, my laboratory has extensive experience with animal models of MS and I will utilize these powerful systems to dissect molecular mechanisms of MS targets and test the therapeutic potential of targeted epigenome editing in vivo. Our findings will set the stage for a paradigm-shift in studying and treating chronic inflammatory diseases based on preventing and modulating aggressive immune responses by inducing self-sustained reversal of aberrant epigenetic states.
Project acronym EvoConBiO
Project Uncovering and engineering the principles governing evolution and cellular control of bioenergetic organelles
Researcher (PI) Iain JOHNSTON
Summary "Complex life on Earth is powered by bioenergetic organelles -- mitochondria and chloroplasts. Originally independent organisms, these organelles have retained their own genomes (mtDNA and cpDNA), which have been dramatically reduced through evolutionary history. Organelle genomes form dynamic populations within present-day eukaryotic cells, akin to individuals co-evolving in a ""cellular ecosystem"". The structure of these populations is central to eukaryotic life. However, the processes shaping the content of these genomes through history, and maintaining their integrity in modern organisms, are poorly understood. This challenges our understanding of eukaryotic evolution and our ability to design rational strategies to engineer bioenergetic performance. EvoConBiO will address these questions using a unique and unprecedented interdisciplinary approach, combining experimental characterisation and manipulation of organelle genomes with mathematical modelling and cutting-edge statistics. This highly novel combination of experiment and theory will drive the field in a new direction, for the first time uncovering the universal principles underlying the evolution and cellular control of mitochondria and chloroplasts. Our groundbreaking recent work on mtDNA suggests a common tension underlying organelle evolution, between genetic robustness (transferring genes to the nucleus) and the control and maintenance of organelles (retaining genes in organelles). EvoConBiO will reveal the pathways underlying organelle evolution, why organisms adapt to different points on these pathways, and how they resolve this underlying tension. In addition to these ""blue sky"" scientific insights into a process of central evolutionary importance, we will harness our findings to ""learn from evolution"" in high-risk high-reward development of new experimental strategies to engineer chloroplast performance in plants and algae of importance in EU agriculture, biofuel production, and bioengineering."
"Complex life on Earth is powered by bioenergetic organelles -- mitochondria and chloroplasts. Originally independent organisms, these organelles have retained their own genomes (mtDNA and cpDNA), which have been dramatically reduced through evolutionary history. Organelle genomes form dynamic populations within present-day eukaryotic cells, akin to individuals co-evolving in a ""cellular ecosystem"". The structure of these populations is central to eukaryotic life. However, the processes shaping the content of these genomes through history, and maintaining their integrity in modern organisms, are poorly understood. This challenges our understanding of eukaryotic evolution and our ability to design rational strategies to engineer bioenergetic performance. EvoConBiO will address these questions using a unique and unprecedented interdisciplinary approach, combining experimental characterisation and manipulation of organelle genomes with mathematical modelling and cutting-edge statistics. This highly novel combination of experiment and theory will drive the field in a new direction, for the first time uncovering the universal principles underlying the evolution and cellular control of mitochondria and chloroplasts. Our groundbreaking recent work on mtDNA suggests a common tension underlying organelle evolution, between genetic robustness (transferring genes to the nucleus) and the control and maintenance of organelles (retaining genes in organelles). EvoConBiO will reveal the pathways underlying organelle evolution, why organisms adapt to different points on these pathways, and how they resolve this underlying tension. In addition to these ""blue sky"" scientific insights into a process of central evolutionary importance, we will harness our findings to ""learn from evolution"" in high-risk high-reward development of new experimental strategies to engineer chloroplast performance in plants and algae of importance in EU agriculture, biofuel production, and bioengineering."
Project acronym EXCHANGE
Project Dynamic Complexes and Allosteric Regulation of Small Molecule Transporters
Researcher (PI) David DREW
Host Institution (HI) STOCKHOLMS UNIVERSITET
Summary Solute Carrier (SLC) transporters mediate the translocation of substrates across membranes and after GPCRs represent the second-largest fraction of the human membrane proteome. SLC transporters are critical to cell homeostasis, which is reflected in the fact that more than a quarter is associated with Mendelian disease. Despite a few exceptions, however, they have been under-utilized as drug targets and most of the mechanistic understanding has been derived from bacterial homologues of these medically important proteins. In addition to subtle differences, bacterial homologues will not enable us to establish how the activities of many SLC transporters are allosterically regulated through the binding of accessory factors, e.g., hormones, to their non-membranous globular domains. Understanding the mechanisms by which their activities can be allosterically regulated through these complex and dynamic assembles is critical to human physiology and important for future drug design. Our model system is a family of transporters known as sodium/proton exchangers (NHEs), which exchange sodium for protons across membranes to aid many fundamental processes in the cell. NHEs are important to the cell cycle, cell proliferation, cell migration and vesicle trafficking and are associated with a wide-spectrum of diseases. Their diverse portfolio is connected to the importance of pH homeostasis, and the binding of many different factors to a large, globular cytosolic domain exquisitely regulates them. To date, we have no structural information for any of the NHE's, functional assays in liposomes are lacking, and many interaction partners are yet to be validated by in vitro studies. Determining the structure, dynamics, and allosteric regulation of NHEs will be an enormous challenge. However, we envisage that by achieving our objectives, we will reveal important mechanistic insights relevant not just to NHEs, but to many types of SLC transporters.
Solute Carrier (SLC) transporters mediate the translocation of substrates across membranes and after GPCRs represent the second-largest fraction of the human membrane proteome. SLC transporters are critical to cell homeostasis, which is reflected in the fact that more than a quarter is associated with Mendelian disease. Despite a few exceptions, however, they have been under-utilized as drug targets and most of the mechanistic understanding has been derived from bacterial homologues of these medically important proteins. In addition to subtle differences, bacterial homologues will not enable us to establish how the activities of many SLC transporters are allosterically regulated through the binding of accessory factors, e.g., hormones, to their non-membranous globular domains. Understanding the mechanisms by which their activities can be allosterically regulated through these complex and dynamic assembles is critical to human physiology and important for future drug design. Our model system is a family of transporters known as sodium/proton exchangers (NHEs), which exchange sodium for protons across membranes to aid many fundamental processes in the cell. NHEs are important to the cell cycle, cell proliferation, cell migration and vesicle trafficking and are associated with a wide-spectrum of diseases. Their diverse portfolio is connected to the importance of pH homeostasis, and the binding of many different factors to a large, globular cytosolic domain exquisitely regulates them. To date, we have no structural information for any of the NHE's, functional assays in liposomes are lacking, and many interaction partners are yet to be validated by in vitro studies. Determining the structure, dynamics, and allosteric regulation of NHEs will be an enormous challenge. However, we envisage that by achieving our objectives, we will reveal important mechanistic insights relevant not just to NHEs, but to many types of SLC transporters.
Project acronym EYELETS
Project A regenerative medicine approach in diabetes.
Researcher (PI) Per-Olof BERGGREN
Summary Pancreatic islet transplantation is essential for diabetes treatment. Outcome varies due to transplantation site, quality of islets and the fact that transplanted islets are affected by the same challenges as in situ islets. Tailor-making islets for transplantation by tissue engineering combined with a more favorable transplantation site that allows for both monitoring and local modulation of islet cells is thus instrumental. We have established the anterior chamber of the eye (ACE) as a favorable environment for long term survival of islet grafts and the cornea as a natural body window for non-invasive, longitudinal optical monitoring of islet function. ACE engrafted islets are able to maintain blood glucose homeostasis in diabetic animals. In addition to studies in non-human primates we are performing human clinical trials, the first patient already being transplanted. Tissue engineering of native islets is technically difficult. We will therefore apply genetically engineered islet organoids. This allows us to generate i) standardized material optimized for transplantation, function and survival, as well as ii) islet organoids suitable for monitoring (sensor islet organoids) and treating (metabolic islet organoids) insulin-dependent diabetes. We hypothesize that genetically engineered islet organoids transplanted to the ACE are superior to native pancreatic islets to monitor and treat insulin-dependent diabetes. Our overall aim is to create a platform allowing monitoring and treatment of insulin-dependent diabetes in mice that can be transferred to large animals for validation. The objective is to combine tissue engineering of islet cell organoids, transplantation to the ACE, synthetic biology, local pharmacological treatment strategies and the development of novel micro electronic/micro optical readout systems for islet cells. This regenerative medicine approach will follow our clinical trial programs and be transferred into the clinic to combat diabetes.
Pancreatic islet transplantation is essential for diabetes treatment. Outcome varies due to transplantation site, quality of islets and the fact that transplanted islets are affected by the same challenges as in situ islets. Tailor-making islets for transplantation by tissue engineering combined with a more favorable transplantation site that allows for both monitoring and local modulation of islet cells is thus instrumental. We have established the anterior chamber of the eye (ACE) as a favorable environment for long term survival of islet grafts and the cornea as a natural body window for non-invasive, longitudinal optical monitoring of islet function. ACE engrafted islets are able to maintain blood glucose homeostasis in diabetic animals. In addition to studies in non-human primates we are performing human clinical trials, the first patient already being transplanted. Tissue engineering of native islets is technically difficult. We will therefore apply genetically engineered islet organoids. This allows us to generate i) standardized material optimized for transplantation, function and survival, as well as ii) islet organoids suitable for monitoring (sensor islet organoids) and treating (metabolic islet organoids) insulin-dependent diabetes. We hypothesize that genetically engineered islet organoids transplanted to the ACE are superior to native pancreatic islets to monitor and treat insulin-dependent diabetes. Our overall aim is to create a platform allowing monitoring and treatment of insulin-dependent diabetes in mice that can be transferred to large animals for validation. The objective is to combine tissue engineering of islet cell organoids, transplantation to the ACE, synthetic biology, local pharmacological treatment strategies and the development of novel micro electronic/micro optical readout systems for islet cells. This regenerative medicine approach will follow our clinical trial programs and be transferred into the clinic to combat diabetes.


text:
Biden's Climate Vision is Too Focused on EVs — Again
Image: PxFuel, CC
Automakers are lining up in support of President Biden's new fuel economy standards that will speed the transition to electric vehicles — but only if Congress passes the car-centric reauthorization and reconciliation bills that will hand Americans billions in free federal money to buy their cars.
In a joint release Thursday, "Big Three" car manufacturers Ford, General Motors and Stellantis celebrated the president's executive order that would roll back former President Trump's loosening of fuel economy standards and set a series of more aggressive standards over the next decade, as part of a larger White House goal to have electric vehicles represent half of new vehicles sold by 2030.
But the triumvirate of automakers also cautioned that it can't accomplish that "dramatic shift" without "the timely deployment of the full suite of electrification policies … including purchase incentives" and other investments that would make plug-in vehicles a realistic solution for more Americans.
Biden seemed open to that feedback, considering that he invited the companies' CEOs to stand alongside him at his press conference about the new fuel standards, and followed up his speech with a few laps in a green megacar on the White House law. (Editor's note: this was not his first joyride.).
Biden zips around WH in hybrid Jeep Wrangler as he pushes for HALF of new cars to be emission-free by 2030 https://t.co/mmCewmqWHU pic.twitter.com/7whAtxuMYa
— Daily Mail US (@DailyMail) August 5, 2021
The automakers' not-so-subtle hint about their federal policy wish list was widely interpreted as a reference to an impending, as-yet-unnamed $3.5 trillion "human" infrastructure budget resolution focused on priorities like housing, healthcare, and daycare — and which many advocates speculate will also tack on billions aimed at tempting drivers to purchase electric cars.
A whopping $100 billion in consumer incentives (and $15 billion more for EV fueling infrastructure) were included in the first draft of Biden's infrastructure stimulus, the American Jobs Plan, but as that legislation morphed into the sprawling Bipartisan Infrastructure Bill, it was whittled down to just $15 billion, split evenly between chargers on interstates and electrifying the nation's bus fleet. Details of the reconciliation bill remain vague, but it is widely expected to restore much of what electric car champions lost in the negotiations — though the same isn't rumored for transit, biking and walking investments, all of which got negotiated down substantially, too.
TFW you remember that America only builds infrastructure if it serves the car culture, corporate hegemony and sprawl. https://t.co/isOehgvzKS
— Streetsblog New York (@StreetsblogNYC) August 5, 2021
That news was met with frustration by mode shift advocates, who say Biden's over-focus on electric vehicles as a silver-bullet climate solution will come at a steep cost to the modes that scientists agree hold the real key to saving the planet without accelerating the national traffic violence crisis: transit, biking and walking.
"While electrifying our personal vehicles is key, that is only part of the solution to our transportation woes," said John Stout of the U.S. Public Interest Research Group. "In addition to garnering the federal government's support for a faster transition to EVs … we need to make it easier for people to drive less."
Bit of a shell game w/ electric cars- it'll reduce smog from cars but it shifts even more power generation to power plants, most of which run on coal, gas, or oil. https://t.co/pxI70uHsiR
— The Electric Strawberry (@KurtFleischer) August 5, 2021
To complicate matters even further, Biden has said he will not sign off on the bipartisan "hard" infrastructure bill, which would mostly fund transportation initiatives, unless Democrats also agree to pass the "human"-infrastructure (with-a-heaping-side-of-electric-cars) bill via reconciliation.
That's bad news all around for progressive advocates dissatisfied with the mixed-bag bipartisan bill. The $1.2-trillion legislation would provide a handful of important wins for active transportation users and a historic $39 billion stimulus for transit agencies, but it would undermine those victories with equally historic spending on new highways. (And again: that's before the fate of the bill was essentially leashed to a massive handout for electric car buyers.)
"You cannot fill a hole with a teaspoon that's still being dug with an excavator," Beth Osborne, director of Transportation for America, said in a statement on the bipartisan legislation. "In its current state, this deal fails to accomplish the administration's goal of reducing emissions, preserving both the status quo of easy money to build new highways (while neglecting basic repair needs) and the existing, complex hurdles to build transit."
This bill also:
• Increases highway $ by historic amounts
• Preserves ability for states to completely ignore repair needs in favor of building new roads
• Keeps transit hard to build
• Lets states continue planning for more people to die on roads each year w no penalties https://t.co/RmKFrXTG9O
— Transportation for America (@T4America) August 5, 2021
Advocates like Osborne are hoping that the bipartisan bill can at least partly be salvaged through the amendment process, which could restore critical provisions like requiring states to at least spend their federal windfall on fixing the highways they've already got before they build more. (Here's a call script on the most urgent amendments from NACTO.)
But if negotiations break down, advocates fear an even worse transportation deal might be brokered under the next Congress. Though Biden's sought-after stimulus dollars for EVs and transit could die without becoming law, lawmakers are legally required to reauthorize the nation's core transportation by 2023, and their next attempt could be disastrous if Democrats lose their triple majority on Capitol Hill, as many think they will.
That leaves sustainable transportation advocates between a rock and an electric Jeep — and America's climate future riding on a pair of deeply imperfect bills that, for better or worse, are the country's best chance at nudging our transportation outlook forward.
Filed Under: Electric Cars, Federal Stimulus, Joe Biden, Reauthorization, Promoted
STREETSBLOG CALIFORNIA
California Takes Victory Lap on Federal EV Goals
By Damien Newton	| Aug 5, 2021
Earlier today, President Joe Biden announced proposed new automobile emission standards and an executive order that will require that half of all cars sold in 2030 and beyond be electric vehicles. The executive order tasks the Environmental Protection Agency (EPA) and National Highway Traffic Safety Administration (NHTSA) to develop aggressive long-term rules to support his […]
Final Obama Fuel-Efficiency Rule Gives Breaks to Electric, Luxury Cars
By Elana Schor	| Apr 1, 2010
The Obama administration today released its final rule raising U.S. auto fuel-efficiency standards to an average of 35.5 miles per gallon (mpg) by 2016, winning plaudits from environmental groups while offering extra benefits to makers of electric and luxury cars. Transportation Secretary Ray Lahood, at left, with EPA chief Lisa Jackson at right. (Photo: Getty […]
New NHTSA Head Was at Center of Pollution Fight
By Melanie Curry	| Jan 22, 2021
It's a strategic move that sends the unequivocal message that the new President is committed to incorporating environmental concerns in every corner of federal policy making.
After Years of Resistance, Auto Industry Agrees to New Mileage Standards
As though to prove compromise isn't as stiff a corpse as it appears, the Obama administration announced on Friday that it had reached a key agreement with an industry proficient at stonewalling government regulation: the automakers. The deal, which meaningfully raises fuel economy standards, was something of a welcome surprise, with the industry putting aside […]
Hold Your Applause For Car Makers' EV Promises
By Kea Wilson	| Feb 3, 2021
General Motors aims to manufacture only electric vehicles by 2035 — but advocates are skeptical that the move will have much of a climate impact without a slate of accompanying policy changes.
Takeaway From Today's EPA Hearing: Fuel Efficiency is a Money-Maker
By Elana Schor	| Oct 21, 2009
A major step towards more fuel-efficient U.S. vehicles is being taken today in Detroit, where the Environmental Protection Agency (EPA) and the U.S. DOT are holding their first in a series of public hearings on the new emissions standards the Obama administration released in May. These GM executives long resisted higher fuel-efficiency standards. Now a […]


Eclipse is a very powerful development environment for Java. Ninite downloads and installs programs automatically in the background. Free download netbeans ide 6 5 1 with jdk. 100% Safe and Secure Free Download ( 32- bit/ 64- bit) Software staller Download Options.
IReport Designer is available as standalone application and as NetBeans plugin for NetBeans IDE 6. Includes Eclipse integration. Jan 16, · Java software allows you to run applications called " applets" that are written in the Java programming language.
0 Update 22 and NetBeans IDE 6. 1 Xdebug , includes the following changes: * PHP support with code completion web service features. Welcome to the NetBeans Plugin Portal. At IDR Solutions we spend a lot of our time working with Java as our JPedal Java PDF Library BuildVu PDF to HTML5/ SVG converter JDeli Java Image Library are written in Java.
Java software allows you to run applications called " applets" that are written in the Java programming language. These applets allow you to have a much richer experience online than simply interacting with static HTML pages. The easiest fastest way to update install software. 1 How to use the Eclipse IDE for Java Application Development. 1 integrated development environment Beans IDE 6. What Java IDE is good for programming? 1 supports animation graphics , media codecs for rich content application development for desktop mobile devices.
Upgrading to the latest Java version improves the security of your system, as older versions do not include the latest security updates. So, here are the complete steps to install Java JDK 11 on Windows 10. » Do I have Java? Free download netbeans ide 6 5 1 with jdk.
It is available for download on Windows macOS Linux based operating systems. The NetBeans IDE is a modular standards- based integrated development environment ( IDE) written in the Java programming language.
Free download netbeans ide 6 5 1 with jdk. 1 with jdk Then try to run the installation again. 1 Download page you can download one of several installers, each of which contains the base IDE additional tools. * New Support for Groovy and netbeans ide 6.
4 32 bit download; Netbeans download for windows 10; Netbeans ide 6. How to Install Java JDK 11 on Windows 10? Download comment, rate plugins provided by community members , third- party companies post your own contributions!
Netbeans free download with jdk; Netbeans for windows 7 32 bit; Netbeans 32 bit windows; Netbeans ide 7. Java Java( tm) File.
5 is a significant update to NetBeans IDE 6. 1 Software Bundle. 1 Java Development Kit. The following installers are available: Java.
Appium Tutorial: Mobile software application' s craze is increasing day by day. Apr 16, · Why download Java? If you find these free tutorials helpful, we would appreciate it if.
Please switch it on before proceeding. If the installation wizard did not find a compatible JDK installation to use with the NetBeans IDE, your JDK is not installed in the default location. This version includes several improvements and bug fixes. Android Studio is the official integrated development environment ( IDE) for Google' s Android operating system built on JetBrains' IntelliJ IDEA software designed specifically for Android development.
Free download netbeans ide 6 5 1 with jdk. 4 download; Jdk netbeans 32 bits; Netbeans 7. Our developers spend a lot of time buried in Java code we use a variety of different IDEs ( Integrated Development Environments) to develop Java code so I. JCreator is a powerful Free IDE for Java.
Development Tools downloads - NetBeans IDE by Oracle Corporation its affiliates , many more programs are available for instant free NetBeans IDE 6. Sixth Edition with runnable code for 20 labs. The basic development system for Java programming is usually referred to as the JDK ( Java Development Kit). Mar 26, · What Java IDE is good for programming? So, here are the complete steps to install Java JDK 11. The various dialects of shell scripts are considered to be scripting languages.
Before installing Java JDK 11, you have to download the Java JDK 11 file. This page is your source to download VM, Java Runtime), also known as the Java plug- in ( plugin), Java Virtual Machine ( JVM, update your existing Java Runtime Environment ( JRE Java VM). Exe 64 bit for free.
The following instructions describe how to install the Java TM 2 Platform, Standard Edition Development Kit ( J2SE 5. Platform Installation The following instructions describe how to install the Java TM Platform, Standard Edition Development Beans IDE 6.
The Eclipse Foundation - home to a global community tools , the Eclipse IDE, over 350 open source projects, Jakarta EE , including runtimes frameworks. Java Plug- in technology, included. By default when you download Eclipse IDE, it doesn' t come with Tomcat install with it.
1 How to use the Eclipse IDE for Java Application Development Java application development is supported by many different tools. Provides tools for developing Java SE Java EE Java ME applications.
0 Update 22 and NetBeans TM IDE 6. For testing point of view, It is very important for us to learn mobile' s software application automation testing to stay connected with new Prepared for the AP * Computer Science Exam in Java.
1 for Windows PC from FileHorse. » Uninstall About Java. On the NetBeans IDE 6. Java Plug- in technology, included as part of the Java 2 Runtime Environment.
EPIC is a set of plugins that turn Eclipse into a full- featured Perl IDE. Java technology allows you to work and play in a secure computing environment. In this tutorial we will go over all. Develop Java and HTML5 applications.
Also peoples are converting there current running software web sites in mobile version and creating apps for mobile application Prepared for the AP * Computer Science Exam in Java. As a student in the College of Engineering complete the first two years of your Engineering major on the Armstrong campus , Information Technology, Computing at the Armstrong campus in Savannah, you may choose to complete your Georgia Southern University bachelor' s degree in Computer Science complete the last two years on the Statesboro campus.
5 download free; Extensions. It is a part of Java SE the Java " Standard Edition" ( as opposed to Java EE for servers Java ME for mobile devices). Java+ You, Download Today! Java Download » What is Java?
J2SE Development Kit 5. Java Tutorial For Beginners: Java Programming From Scratch download, Configuring, nfiguring & Using Apache Tomcat A Tutorial on Installing, Using Tomcat for JSF , learn free java tutorial class sun beginner programming tutorials, learn, Servlet , Java Programming - Step by Step tutorial JSP Development. 0 Update 22 JDK TM) and the NetBeans TM IDE 6.
1 Software Bundle J2SE TM Development Kit 5. The NetBeans project consists of an open source IDE an application platform which can be used as a generic framework to build any kind of Beans IDE 6. This tutorial is a guide to installing configuring Apache Tomcat 6 , servlets JSP. A shell script is a computer program designed to be run by the Unix shell, a command- line interpreter.
Featured News: Apache NetBeans ( incubating) 10 Released; What' s Happened to My Favorite NetBeans Plugins? 5M Lines of NetBeans Code from Oracle to Apache.
Apache NetBeans Releases Page. Apache NetBeans 11.
0 is the latest version of the IDE. It was released on April 4,.
The most intelligent Ruby on Rails IDE with rails code refactorings and code completion. A free Java Development Environment designed for beginners, used by millions worldwide.
" One of my favourite IDEs out there is BlueJ" — James Gosling, creator of Java. Java EE Tools for creating Java web and enterprise applications compatible with Java EE 6, Java EE 5 and J2EE 1.
Includes support for servlets, JSPs, tag libraries, Java Server Faces, Java Persistence API, Enterprise Java Beans, JAX- WS and RESTful web services, Spring and Beans IDE 6. 0 introduced support for developing IDE modules and rich client applications based on the NetBeans platform, a Java Swing GUI builder ( formerly known as " Project Matisse" ), improved CVS support, WebLogic 9 and JBoss 4 support, and many editor enhancements. NetBeans 6 is available in official repositories of major Linux distributions.
Additional Resources; JDK 8 with NetBeans This distribution of the JDK includes the NetBeans IDE, which is a powerful integrated development environment for developing applications on the Java platform. : Oracle Java Advanced Management Console Advanced Management Console ( AMC) enables desktop administrators to track and manage Java usage across their organization - - ep intelligence. After IntelliJ IDEA has indexed your source code, it offers a blazing fast and intelligent experience by giving relevant suggestions in every context: instant and clever code completion, on- the- fly code analysis, and reliable refactoring tools.


but any woman who prays or prophesies with her head unveiled dishonors her head — it is the same as if her head were shaven.
For if a woman will not veil herself, then she should cut off her hair; but if it is disgraceful for a woman to be shorn or shaven, let her wear a veil.
(For man was not made from woman, but woman from man.
That is why a woman ought to have a veil on her head, because of the angels.
Judge for yourselves; is it proper for a woman to pray to God with her head uncovered?
but if a woman has long hair, it is her pride? For her hair is given to her for a covering.
If any one is disposed to be contentious, we recognize no other practice, nor do the churches of God.
Catholic women, therefore, cover their heads while praying and while inside a church in order to honor themselves and to honor God. Likewise, Catholic men make certain that their heads are uncovered when they are praying or when they are inside a church.
UVNA designed a set of altar cards for our Mass kit, which we use when we help organize Masses in churches that may lack all of the liturgical hardware for the Traditional Latin Mass. The altar cards are travel size: the center card is 10×20 inches, and the side cards are 8×10 inches. We find that with these sizes the two side cards neatly stack on top of the center card side-by-side when transporting. The artwork used in the center card is from a Crucifixion Diptych by Rogier van der Weyden (1460).
We recommend getting the cards professionally printed on matte paper, which should only cost about $15. While 10×20 frames are generally not considered to be of standard size. they can sometimes be found ready-made at some crafts stores.
Please let us know if you find these altar cards to be useful!


When it comes to activewear tops, Rockwear's got your back and front. Keep your top half in check with our range of women's activewear tops. Ranging from throw-over gym shirts to midriff tops, support singlets to hoodies, we've got your style, size, design (and chest!) covered.


Perfect for keeping in your car or carrying with you, Wellness bags are the ideal shopping companion since they take up very little space but fold out into large sized tote bags. The handles are very comfortable, and the Wellness bags are all crafted to be extremely durable, as well as hold a lot of weight.
This bag is made from a soft light pink silk with a radiating circle polka dot pattern on a black vinyl bottom.
There is a contrasting hot pink outer zipper and an outside bright pink pocket for storing coins, money, credit cards or ID, etc.
The bag is 16" in height and 18" wide.
The folded size is approximately 1 1/2" by 5" by 5 1/2".


text:
November 13, 2015 November 13, 2015 T-bone News
Star Wars Musical is a fan film that spoofs Star Wars as a Disney Musical, colliding two of the galaxy's biggest fandoms. Having completed the first episode which recently won the Best Short Film award at Stan Lee's Comikaze GeekFest Film Festival, the team behind this mashup is crowdfunding their next episode and are excited to be chosen to be a part of Rockethub and Ovation TV's Creative Studio, where they compete for a chance to be featured on TV and win $5000 towards funding the project.
You can watch the production here: http://youtu.be/8xzGSSN5FNE or at StarWarsMusical.com
Star Wars Musical achieved viral success with over 250,000 views and restless excitement for the following sequel. With solidifying plans for Star Wars Musical's take on Episode V: Empire Strikes Back just around the corner, executive director and composer George Shaw is seeking bigger aspirations for his musical project. The team is aiming to release their adaptation, entitled "Star Wars Musical: The Empire's New Groove" by December 2016, with the support of devoted Star Wars and Disney fans!
Through this campaign, the team hopes to bring their passion project to a whole new level in terms of quality and production and present it to fans who share the same affection for their geekdoms worldwide.
Support the crowdfunding campaign here: http://bit.ly/StarWarsMusical
Rewards video on Instagram: https://instagram.com/p/9TsWCqumJe/
HOW TO SUPPORT:
1. Feature Empire's New Groove's crowdfunding campaign on your blog, and let me know when you have done so!
2. Reply to my email to set up an exclusive one-on-one interview with composer George Shaw and/or director Jeffrey Gee Chin.
3. Fund our campaign and share the love on social media!
4. Enter to win a T-shirt in our Star Wars Musical Photoshop contest! Create a cover photo for our page using at least one of our images – download all of images here. Post your entry at http://facebook.com/thestarwarsmusical. Deadline is Nov. 19th.
ABOUT THE CREATORS
Composer George Shaw recently co-wrote "Ode to Geeks" with Marvel Comics legend Stan Lee and produced a music video featuring a chorus of geek voices assembled across the internet. He also composes for Geek & Sundry's Tabletop show hosted by Wil Wheaton (Star Trek: The Next Generation), music directs the Geekie Awards in his 3rd year, and created the viral hit Star Wars Musical out of his passion for Star Wars and Disney. He followed it up with Netflix Musical,which stars Harry Shum Jr. (Glee) and Chrissa Sparkles. YouTube videos featuring George's music have amassed over 150 million views.
Director Jeffrey Gee Chin has been featured on Good Morning America and at the U.S. Embassy in Seoul, Korea for his film work. In 2012, he completed his first narrative short filmLil Tokyo Reporter, which starred Academy Award-Winner Chris Tashima as Civil Rights Leader Sei Fujii. The film won over 20 awards and was finished at Sony Pictures Studios. As a USC graduate student, Chin VFX supervised a Student Academy Award Finalist Drone and completed a feature adaptation of James Franco's Actor's Anonymous as a 2nd-Unit Director and Visual Effects Supervisor. Chin also recently edited an Emmy nominated show for San Francisco Government Television.
Find more exclusive behind-the-scenes content and updates for Star Wars Musical at: http://starwarsmusical.com
disney music musical
Notice: It seems you have Javascript disabled in your Browser. In order to submit a comment to this post, please write this code along with your comment: 94d6e3f7955701a409ef11616ea7aefc


Bike has approx 5,500 mis on Polished 100 Cu. In. RevTech engine and six gear trans. Dyne 2000 I ignition, Custom oil cooler, Mean Mothers pipes, Spyke High Torque chrome starter, Buff Fat Bars, Ness forward controls, Has spare two up seat, quick disconnect winshield and sissy bar.