text:
Cells, CulturedCell LineCytosolCell MembraneMyocardiumTumor Cells, CulturedBrainNeuronsCell Line, TumorMuscle, Smooth, VascularHeLa CellsCell NucleusHippocampusFibroblasts3T3 CellsSubcellular FractionsLiverCytoplasmHEK293 Cells
Paramecium tetraureliaCattleRats, Sprague-DawleyRabbitsRats, WistarSwineSaccharomyces cerevisiaeArabidopsisMice, Inbred C57BLCricetinaeMice, KnockoutEscherichia coliRats, Inbred Strains
Chemicals and Drugs 165
Cyclic AMPProtein KinasesProtein Kinase CCyclic AMP-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein KinasesProtein Kinase InhibitorsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein Kinase Type 1CalciumCyclic GMP-Dependent Protein KinasesProtein-Serine-Threonine KinasesCalmodulinMitogen-Activated Protein KinasesIsoenzymesp38 Mitogen-Activated Protein KinasesPhosphatidylinositol 3-KinaseseIF-2 KinaseEnzyme InhibitorsCyclic GMPTetradecanoylphorbol AcetateProtein Kinase C-alphaMitogen-Activated Protein Kinase 1Cyclic AMP-Dependent Protein Kinase Catalytic SubunitsCyclic AMP-Dependent Protein Kinase Type IIMitogen-Activated Protein Kinase KinasesNucleotides, CyclicMitogen-Activated Protein Kinase 3Protein Kinase C-deltaBucladesineColforsinIsoquinolinesCalcium-Binding ProteinsJNK Mitogen-Activated Protein KinasesPhosphoprotein PhosphatasesPeptide TAMP-Activated Protein KinasesAdenosine TriphosphateCyclic GMP-Dependent Protein Kinase Type I1-(5-Isoquinolinesulfonyl)-2-MethylpiperazinePhosphoproteinsSerineCyclic AMP-Dependent Protein Kinase RIalpha Subunit8-Bromo Cyclic Adenosine MonophosphateReceptors, Cyclic AMPProtamine KinaseBenzylaminesProtein Kinase C-epsilonsrc-Family KinasesProtein Kinase C betaCalcium-Calmodulin-Dependent Protein Kinase Type 4Cyclic GMP-Dependent Protein Kinase Type IIRNA, MessengerRecombinant Fusion ProteinsProtein-Tyrosine KinasesRecombinant ProteinsCarrier ProteinsPhospholipidsCDC2 Protein KinaseSulfonamides1-Methyl-3-isobutylxanthineCasein Kinase IIPhosphatidylserinesProteinsMAP Kinase Kinase KinasesMembrane ProteinsRNA, Double-StrandedExtracellular Signal-Regulated MAP KinasesAdenylate CyclasePeptides3',5'-Cyclic-AMP PhosphodiesterasesIntracellular Signaling Peptides and Proteinsp21-Activated KinasesCyclic AMP Response Element-Binding ProteinMAP Kinase Kinase 1Proto-Oncogene Proteins c-aktProto-Oncogene ProteinsMagnesiumRibosomal Protein S6 KinasesSirtuinsPlant ProteinsCyclic AMP Receptor ProteinDiglyceridesDNA-Binding ProteinsPhorbol 12,13-DibutyrateEgtazic AcidSirtuin 1MAP Kinase Kinase 4Casein KinasesTheophyllineStaurosporinePhorbol EstersTranscription FactorsCyclin-Dependent KinasesMaleimidesIndolesIsoproterenolPyruvate KinaseGlycogen Synthase Kinase 3Phosphotransferases (Alcohol Group Acceptor)Creatine KinaseArabidopsis ProteinsDNA-Activated Protein KinaseNuclear ProteinsPeptide FragmentsAdenosine MonophosphateTyrosineFlavonoids3-Phosphoinositide-Dependent Protein KinasesAMP DeaminaseReceptor Protein-Tyrosine KinasesPhosphodiesterase InhibitorsSaccharomyces cerevisiae ProteinsRNA, Small InterferingThreoninerho-Associated KinasesAndrostadienesDNA PrimersAlkaloidsImidazolesPyridinesCell Cycle ProteinsPhosphoserineCarbazolesMitogen-Activated Protein Kinase 8I-kappa B KinaseTrifluoperazineThymidine KinaseCalcimycinRibosomal Protein S6 Kinases, 90-kDaReceptors, N-Methyl-D-AspartateInsulinCDC2-CDC28 KinasesGlucoseChromonesMAP Kinase Kinase Kinase 1DNA, ComplementaryCalcineurin1-Phosphatidylinositol 4-KinaseMorpholinesFungal ProteinsAurora KinasesMAP Kinase Kinase 2Diacylglycerol KinaseThionucleotidesProtein IsoformsType C PhospholipasesDNAAdaptor Proteins, Signal TransducingBenzophenanthridinesNF-kappa BMyosin-Light-Chain KinaseNerve Tissue ProteinsMultienzyme ComplexesCalcium-Calmodulin-Dependent Protein Kinase KinaseEnzyme ActivatorsA Kinase Anchor ProteinsDeath-Associated Protein KinasesFocal Adhesion Kinase 1ButadienesProtein Phosphatase 2TOR Serine-Threonine KinasesMAP Kinase Kinase 6Calmodulin-Binding ProteinsMacromolecular SubstancesEpidermal Growth Factor
Analytical, Diagnostic and Therapeutic Techniques and Equipment 12
Blotting, WesternTransfectionElectrophoresis, Polyacrylamide GelModels, BiologicalCloning, MolecularImmunoblottingMutagenesis, Site-DirectedPrecipitin TestsSequence AlignmentModels, MolecularPeptide MappingReverse Transcriptase Polymerase Chain Reaction
PhosphorylationEnzyme ActivationSignal TransductionKineticsMAP Kinase Signaling SystemAmino Acid SequenceSubstrate SpecificityProtein BindingMutationBase SequenceBinding SitesTransfectionDose-Response Relationship, DrugTime FactorsGene Expression RegulationMolecular WeightGene Expression Regulation, EnzymologicTranscription, GeneticSequence Homology, Amino AcidApoptosisCell DivisionCalcium SignalingProtein Structure, TertiaryCatalytic DomainRNA, Double-StrandedProtein TransportPromoter Regions, GeneticCell CycleCell SurvivalCell DifferentiationGene ExpressionSecond Messenger SystemsUp-RegulationBiological TransportDown-RegulationProtein Processing, Post-TranslationalPlasmidsStructure-Activity RelationshipTranscriptional ActivationStimulation, ChemicalEnzyme InductionCatalysisCell Proliferation
Molecular Sequence DataAmino Acid SequenceBase Sequence
Does Protein Kinase A-Mediated Phosphorylation of the Cardiac Ryanodine Receptor Play Any Role in Adrenergic Regulation of...
Cardiac myocyte ryanodine receptors (sarcoplasmic reticulum [SR] Ca release channel; cardiac ryanodine receptor [RyR2]) are localized in the junctional SR, in close proximity to L-type Ca channels (LTCCs) embedded in the membranes of the transverse (T)-tubules. This signaling microdomain has been termed the couplon,1 because it is here that excitation-contraction coupling takes place. As the heart fills with blood during diastole, RyR2 is stabilized in a closed state, allowing Ca uptake by the SR Ca ATPase to pump Ca from the cytoplasm into the SR, providing the primary source of Ca to activate the contractile apparatus during the next heart beat (systole). During systole, the cardiac action potential depolarizes the T-tubules and causes the opening of LTCCs. Ca influx through LTCCs elevates the [Ca] within the cytoplasmic space between the T-tubular and SR membrane, which promotes Ca binding to neighboring RyR2s, inducing some to open. Ca then moves out of the SR lumen into the subsarcolemmal, ...
Adenosine suppresses protein kinase A- and C-induced enhanceme...
Adenosine suppresses protein kinase A- and C-induced enhancement of glutamate release in the hippocampus.: Cultured hippocampal neurons from neonatal rats were
PDE4B mediates local feedback regulation of β1-adrenergic cAMP signaling in a sarcolemmal compartment of cardiac myocytes |...
The data described above demonstrate a local PDE feedback regulation in cardiac myocytes whereby β1AR signals activate PDE4B3 via PKA-mediated phosphorylation. PKA-activated PDE4B, in turn, reduces the local steady-state cAMP concentration in a confined subsarcolemmal domain. The functional consequences of interrupting this feedback include increased PKA-mediated phosphorylation of Cav1.2 and RyR2, which is necessary for fine-tuning of ECC, a finding consistent with the cardiac phenotype of PDE4BKO mice (Leroy et al., 2011). Functional data on intracellular Ca2+ levels and contraction rate confirm the altered cAMP/PKA signaling in PDE4BKO myocytes. These conclusions are based on measurements of βAR-induced cAMP accumulation at the plasma membrane of PDE4BKO myocytes, the effects of acute PDE4B-selective inhibition in wild-type myocytes, the β1AR-dependent activation of PDE4B and the altered PKA-mediated phosphorylation of some, but not all substrates involved in Ca2+ homeostasis and ...
Plus it
Voltage-gated sodium channels, which initiate action potentials in mammalian brain neurons, are modulated functionally by cAMP-dependent protein kinase A (PKA), resulting in reduced sodium current amplitude. Comparing brain and muscle sodium channels, we show that only the brain channel is modulated by PKA. The brain sodium channel I-II linker is both necessary and sufficient for PKA modulation, as shown by exchanging the I-II linker regions of the two channels. PKA consensus sites in the brain channel I-II linker were eliminated by deletion and site-specific mutagenesis. The mutant channels demonstrated decreased levels of phosphorylation when metabolically labeled in oocytes with [gamma-32P]-ATP, and they did not respond with a reduction in current magnitude after PKA induction. Modulation of the brain channel by PKA phosphorylation was mimicked by adding fixed negative charges at the PKA consensus sites, suggesting that the decrease in current was a direct result of the negative charge at one ...
A dual-specificity isoform of the protein kinase inhibitor PKI produced by alternate gene splicing | Biochemical Journal
We have previously shown that the protein kinase inhibitor β (PKIβ) form of the cAMP-dependent protein kinase inhibitor exists in multiple isoforms, some of which are specific inhibitors of the cAMP-dependent protein kinase, whereas others also inhibit the cGMP-dependent enzyme [Kumar, Van Patten and Walsh (1997), J. Biol. Chem. 272, 20011-20020]. We have now demonstrated that the switch from a cAMP-dependent protein kinase (PKA)-specific inhibitor to one with dual specificity arises as a consequence of alternate gene splicing. We have confirmed using bacterially produced pure protein that a single inhibitor species has dual specificity for both PKA and cGMP-dependent protein kinase (PKG), inhibiting each with very high and closely similar inhibitory potencies. The gene splicing converted a protein with 70 amino acids into one of 109 amino acids, and did not change the inhibitory potency to PKA, but changed it from a protein that had no detectable PKG inhibitory activity to one that now ...
TPK3 - cAMP-dependent protein kinase type 3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - TPK3 gene...
cAMP-dependent protein kinase complex, cytoplasm, nucleus, cAMP-dependent protein kinase activity, mitochondrion organization, protein kinase A signaling, protein phosphorylation, Ras protein signal transduction
cAMP-dependent protein kinase catalytic subunit beta
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis ...
Protein Kinase A-Dependent Derepression of the Human Prodynorp...
Protein Kinase A-Dependent Derepression of the Human Prodynorphin Gene via Differential Binding to an Intragenic Silencer Element: Induction of the prodynorphin
Distal Recognition Sites in Substrates Are Required for Efficient Phosphorylation by the cAMP-Dependent Protein Kinase |...
Protein kinases are able to recognize their appropriate targets in a complex milieu of cellular protein. This process must be carried out with high fidelity to ensure proper signal transduction in eukaryotic cells (Hunter 2000). In this study, we attempted to obtain insight into this recognition by examining PKA variants that exhibit a stable association with substrates. This binding provided a facile assay that allowed us to identify domains in both enzyme and substrates that were important for PKA phosphorylation. The substrate domains identified were physically removed from the sites of phosphorylation and were required for efficient recognition by PKA both in vivo and in vitro. To the best of our knowledge, these studies are the first to show that such distal sequence elements in substrates are required for phosphorylation by PKA. These observations may help explain why only a fraction of proteins that contain a PKA consensus site are phosphorylated by this enzyme in vivo (Budovskayaet al. ...
A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging
Neuromodulators have profound effects on behavior, but the dynamics of their intracellular effectors has remained unclear. Most neuromodulators exert their function via G-protein-coupled receptors (GPCRs). One major challenge for understanding neuromodulator action is the lack of dynamic readouts of the biochemical signals produced by GPCR activation. The adenylate cyclase/cyclic AMP/protein kinase A (PKA) module is a central component of such biochemical signaling. This module is regulated by several behaviorally important neuromodulator receptors. Furthermore, PKA activity is necessary for the induction of many forms of synaptic plasticity as well as for the formation of long-term memory. In order to monitor PKA activity in brain tissue, we have developed a 2-photon fluorescence lifetime imaging microscopy (2pFLIM) compatible PKA sensor termed FLIM-AKAR, which is based on the ratiometric FRET sensor AKAR3. FLIM-AKAR shows a large dynamic range and little pH sensitivity. In addition, it is a ...
Dancing with Different Partners: Protein Kinase A Phosphorylation of Seven Membrane-Spanning Receptors Regulates Their G...
The first indication that PKA-mediated phosphorylation of the β2AR might do more than simply "desensitize" the receptor by inhibiting its coupling to Gs was provided by Okamoto et al. (1991). They demonstrated that a short peptide derived from the third cytoplasmic loop of the β2AR effectively activated purified Gs but only very weakly activated Gi in vitro. This peptide contains one of the two consensus PKA phosphorylation sites found in the receptor. When the peptide was phosphorylated by PKA, its ability to activate Gs was dramatically reduced, whereas its ability to activate Gi was reciprocally increased.. These results are essentially identical to findings in recent in vitro reconstitution studies with the intact recombinant human β2AR and recombinant Gsand Gi (Zamah et al., 2002). Reconstituted native β2AR mediates robust activation of Gs but not Gi. When the receptor is phosphorylated in vitro by PKA, Gscoupling is reduced, but Gi activation is markedly enhanced. The results are ...
The major objective of the present study was to further our understanding of the molecular mechanisms by which perturbations of the cAMP pathway regulate PKA subunit expression in neural cells. Indeed, the cAMP-induced down-regulation of PKA subunits described here contrasts with the findings reported for non-neural systems. In Sertoli cells, an extensively studied model system for PKA regulation (reviewed in Skålhegg and Taskén, 1997), activation of the cAMP pathway elevates PKA subunit protein levels along with a 2- to 4-fold increase in RIα, RIIα and Cα mRNA and a 50-fold increase in RIIβ mRNA. This up-regulation of mRNA levels involved both increased transcription and increased mRNA stability. In mouse epithelial cells (Lange-Carter and Malkinson, 1991), elevated levels of cAMP resulted in a similar increase in RIIβ mRNA but a decrease in mRNA for RIα and no change in that for RIIα. Earlier studies have suggested cAMP stimulated proteolytic degradation of C, but not R, subunits in ...
PKA signal | PKA signal is required for initiation | Page 2
42 of the Chromas software package (Conor McCarthy, Southport, Australia). For all analyses, data obtained PLX3397 purchase with the forward and reverse primers were combined and aligned to the consensus sequence obtained from the BLAST GenBank database http://www.ncbi.nlm.nih.gov/nuccore/166706780?report=genbank. Figure 1 Sequencing of the KRAS gene in DNA isolated from NSCLC tissues. (A) Wild type-(12Gly-GGT, 13Gly-GGC), (B) Mutant- (12Asp-GAT). Pyrosequencing In the pyrosequencing method for DNA sequence analysis [16, 17], inorganic phosphate released in the course of nucleotide incorporation serves as the initial substrate in a sequence of four. successive enzymatic reactions. This result in the emission of light, which functions as a signal that is proportional to the number of nucleotides incorporated. In this project, the PyroMark K-ras assay test (Biotage, Uppsala, Sweden) was used for primary amplification P005091 cost and pyrosequencing of both the 12th and ...
JCI - Altered intracellular Ca2+ handling in heart failure
Intracellular Ca2+ cycling and associated signaling pathway in cardiomyocytes. On a beat-by-beat basis, a calcium transient is evoked by the initial influx of a small amount of Ca2+ through the LTCC and the subsequent large-scale Ca2+ release from the SR through the RyR. During diastole, cytosolic Ca2+ is taken up into the SR by the PLN-regulated SERCA2a pump. β receptor-mediated PKA stimulation regulates this Ca2+ cycling by phosphorylating LTCC, RyR, and PLN. In normal hearts, sympathetic stimulation activates β1-adrenergic receptor, which in turn stimulates the production of cAMP by adenylyl cyclase and thereby activates PKA. PKA phosphorylates PLN and RyR, both of which contribute to an increased intracellular Ca2+ transient and enhanced cellular contractility (pink zone signal). PP1 and PP2A regulate the dephosphorylation process of these Ca2+ regulatory proteins (RyR, PLN, LTCC) (blue zone signaling). Activation of the Gαq-coupled receptors (angiotensin II receptor, endothelin 1 ...
A Single Second Messenger | Circulation Research
Second, the biological response to a second messenger can depend on the scaffolding complex that binds enzymes that synthesize or degrade the second messenger, and effectors (Figure 1B). There are numerous examples of this mode of operation in cells. A-kinase anchoring proteins (AKAPs) are known to localize cAMP-dependent protein kinase (PKA) to different subcellular compartments in the cell, thereby ensuring phosphorylation of PKA targets in the correct cellular vicinity.16 Some AKAPs have also been found to associate with cAMP-hydrolyzing PDE isoforms, thereby providing a mechanism for fine-tuning local cAMP levels and downstream effects of PKA.17 The PDE4D splice variant PDE4D3 binds to muscle-selective AKAP (mAKAP), and this association results in low cAMP levels and prevents PKA activation under basal conditions. Following stimulation of cAMP synthesis, however, PKA is activated and phosphorylates mAKAP. These phosphorylation events result in a more efficient PDE4D3 action, and provides a ...
Modeling Oscillations in the cAMP-PKA Network Within Budding Yeast
In our work we develop and analyze an ordinary differential equation. model that describes the cyclic adenosine monophosphate (cAMP) --Protein Kinase A (PKA) pathway in budding yeast. In particular our. model describes the effect of glucose stimulation on the concentration of cAMP in the short term,. and the effect of stress in the long term. We develop this model in. order to understand two specific experimental results, reported by. Ma et al. (1999) and Garmendia-Torres et al. (2007). In order to describe the. surprising results published by Ma et al. (1999) we make a key assumption. that three enzymes within the cAMP-PKA network compete with one. another for activation by PKA. This assumption sets our model apart. from previous models of the cAMP-PKA network.. Our model focuses on two forms of negative feedback that. drive oscillations in the concentration of cAMP. Under high or low. stress conditions (for example, following glucose stimulation) our model reduces to a single ...
Oral Presentation - ASN Events
The cyclic AMP-dependent protein kinase, PKA, has been proposed to regulate many aspects of β-cell biology, including insulin secretion, insulin synthesis, β-cell survival and β-cell proliferation. We have established β-caPKA mice, a model of tamoxifen-inducible PKA activity that is targeted specifically to the islet β-cells. We have shown that enhanced insulin secretion in β-caPKA mice tightens glucose control and that this not dependent upon increased β-cell mass. Enhanced insulin secretion and improved glucose control was maintained to 52 weeks of age but β-cell mass at 52 weeks of age did not differ between β-caPKA mice and littermate controls, indicating again that activation of PKA in the islet β-cells does not promote β-cell proliferation. To determine whether PKA activity is protective for β-cells, PKA activity was induced in β-caPKA mice, then streptozotocin was administered to induced β-cell destruction. Increasing PKA activity in β-cells protected them from ...
Protein Lounge: PKA Signaling
|P>PKA (Protein Kinase-A) is an enzyme that regulates processes as diverse as growth, development, memory, and metabolism. In its inactivated state, PKA exists as a tetrameric complex of two Catalytic subunits (PKA-C) and a Regulatory (PKA-R) subunit dimer. To date, [...]
RCSB PDB - Protein Feature View - cAMP-dependent protein kinase inhibitor alpha - P63249 (IPKA RAT)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
RCSB PDB - 1BKX: A BINARY COMPLEX OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN KINASE AND ADENOSINE FURTHER...
1BKX: A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility.
Mechanisms governing the compartmentalization of activated PKA - Shane Tillo
In the hippocampus, the cAMP dependent protein kinase (PKA) plays critical roles in neurotransmission, cell excitability, and synaptic plasticity.All of these p...
Assembly and regulation of dynamic PKA macromolecular signaling systems - Susan Taylor
cAMP-dependent protein kinase (PKA), ubiquitous in mammalian cells, regulates a plethora of cell processes including development, differentiation, memory, and m...
The Dysfunction of Camp-Dependent Na+/Ca2+ Exchange in Reverse Mode as a Primary Mechanism for Age-Dependent Cardio-Muscle...
Sinerik Ayrapetyan-The Dysfunction of Camp-Dependent Na+/Ca2+ Exchange in Reverse Mode as a Primary Mechanism for Age-Dependent Cardio-Muscle Failure
Protein kinase A-anchoring inhibitor peptides arrest mammalian sperm motility<...
TY - JOUR. T1 - Protein kinase A-anchoring inhibitor peptides arrest mammalian sperm motility. AU - Vijayaraghavan, Srinivasan. AU - Goueli, Said A.. AU - Davey, Michael. AU - Carr, Daniel. PY - 1997/2/21. Y1 - 1997/2/21. N2 - Cyclic AMP-dependent protein kinase (PKA) is anchored at specific subcellular sites through the interaction of the regulatory subunit (R) with protein kinase A-anchoring proteins (AKAPs) via an amphipathic helix binding motif. Synthetic peptides containing this amphipathic helix domain competitively disrupt PKA binding to AKAPs and cause a loss of PKA modulation of cellular responses. In this report we use S-Ht31, a cell-permeant anchoring inhibitor peptide, to study the role of PKA anchoring in sperm. Our analysis of three species of mammalian sperm detected three isoforms of PKA (RIIα, RIIβ, and RIβ) and one 110-kDa AKAP. The addition of S-Ht31 to bovine caudal epididymal sperm inhibits motility in a time- and concentration-dependent manner. A control peptide, ...
CAMP-dependent protein kinase | definition of cAMP-dependent protein kinase by Medical dictionary
Looking for online definition of cAMP-dependent protein kinase in the Medical Dictionary? cAMP-dependent protein kinase explanation free. What is cAMP-dependent protein kinase? Meaning of cAMP-dependent protein kinase medical term. What does cAMP-dependent protein kinase mean?
The Involvement of Tyrosine Kinases, Cyclic AMP/Protein Kinase A, and p38 Mitogen-Activated Protein Kinase in IL-13-Mediated...
In the present study we demonstrated that IL-13, a Th2 cell-derived cytokine, is a potent arginase activator, and its induction of arginase contributes significantly to the suppression of NO production in LPS-activated macrophages. The increase in arginase activity is a result of de novo synthesis of arginase I mRNA and protein. Studies on the signaling molecules involved in arginase activation show that a surge in intracellular cAMP and the subsequent activation of PKA are obligatory for arginase induction. In addition, tyrosine kinases and p38 MAPK play a role in IL-13-induced arginase activation. To provide a perspective on our observations and conclusions, the results from the present study are discussed below in reference to previous findings regarding the signaling pathways involved in arginase activation and NO regulation by arginase.. In the present study despite the basal level of arginase I gene expression being detected in resting macrophages, arginase protein expression and enzyme ...
Prostaciklinski receptor - Wikipedia
Lawler OA, Miggin SM, Kinsella BT (2001). "Protein kinase A-mediated phosphorylation of serine 357 of the mouse prostacyclin receptor regulates its coupling to G(s)-, to G(i)-, and to G(q)-coupled effector signaling.". J. Biol. Chem. 276 (36): 33596-607. PMID 11443126. doi:10.1074/jbc.M104434200. ...
Characterization of the bovine lens plasma membrane substrates for cAMP‐dependent protein kinase<...
TY - JOUR. T1 - Characterization of the bovine lens plasma membrane substrates for cAMP‐dependent protein kinase. AU - LOUIS, Charles F.. AU - JOHNSON, Ross. AU - JOHNSON, Keith. AU - TURNQUIST, Janet. PY - 1985/7. Y1 - 1985/7. N2 - cAMP‐dependent protein kinase, derived from either calf lens or bovine heart, promotes the phosphorylation of three lens plasma membrane proteins of molecular mass 28 kDa, 26 kDa and 18 kDa. Correlation of the maximal level of phosphorylation of these components with the Coomassie blue staining intensity of fractionated lens membranes suggests that the phosphorylation of the 28 kDa and 18 kDa components may be approximately stoichiometric. The protein kinase substrates could be dephosphorylated by a cardiac sarcoplasmic‐reticulum‐bound protein phosphatase activity. The 26 kDa component comigrated with MP26, the major lens membrane component that has been localized to the lens fiber cell junction. Treatment of phosphorylated lens membranes with chymotrypsin ...
5N3F | CAMP-DEPENDENT PROTEIN KINASE A FROM CRICETULUS GRISEUS IN COMPLEX WITH FRAGMENT LIKE MOLECULE N-[3-(AMINOMETHYL)PHENYL...
cansSAR 3D Structure of 5N3F | CAMP-DEPENDENT PROTEIN KINASE A FROM CRICETULUS GRISEUS IN COMPLEX WITH FRAGMENT LIKE MOLECULE N-[3-(AMINOMETHYL)PHENYL]ACETAMIDE | 5N3F_A | cAMP-dependent protein kinase catalytic subunit alpha - Also known as KAPCA_CRIGR, PRKACA. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B
cAMP-dependent protein kinase type I-beta regulatory subunit
The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015 ...
The Ustilago maydis regulatory subunit of a cAMP-dependent protein kinase is required for gall formation in maize. | Plant Cell
In the plant, filamentous growth is required for pathogenicity of the corn smut pathogen Ustilago maydis. Earlier, we identified a role for the cAMP signal transduction pathway in the switch between budding and filamentous growth for this fungus. A gene designated ubc1 (for Ustilago bypass of cyclase) was found to be required for filamentous growth and to encode the regulatory subunit of a cAMP-dependent protein kinase (PKA). Here, we show that ubc1 is important for the virulence of the pathogen. Specifically, ubc1 mutants are able to colonize maize plants and, like the wild-type pathogen, cause localized symptoms in association with the presence of hyphae. However, in contrast to plants infected with wild-type cells that often developed galls from initially chlorotic tissue, plants infected with the ubc1 mutant did not produce galls. These data suggest that PKA regulation is critical for the transition from saprophytic to pathogenic growth and from vegetative to reproductive development. Plate ...
Endogenous substrate proteins for Ca2+-calmodulin-dependent, Ca2+-phospholipid-dependent and cyclic AMP-dependent protein...
The occurrence of endogenous substrate proteins for Ca2+-dependent protein kinase, augmented by either phospholipid or calmodulin, and for cyclic AMP-dependent protein kinase was examined in homogenates and subcellular fractions of mouse pancreatic islets. Islet protein phosphorylation was enhanced by Ca2+-calmodulin; the major endogenous substrates in the homogenate were two proteins of Mr 53000 and 100000. The Mr-100000 phosphoprotein was localized to a 27000g-supernatant fraction, whereas the Mr-53000 phosphoprotein was present in a 27000g particulate fraction of mouse islets. In the presence of Ca2+, phosphatidylserine stimulated phosphorylation of 15 proteins, of Mr 17000-190000, in a 27000g-supernatant fraction. No effects of Ca2+ plus phosphatidylserine were observed in a 27000g particulate fraction of mouse islets. Examination of cyclic AMP-dependent protein phosphorylation revealed five substrate proteins, of Mr 23000-72000, present in the 27000g supernatant of mouse islets. No common ...
Cloning, characterization, and expression of the gene for the catalytic subunit of cAMP-dependent protein kinase in...
TY - JOUR. T1 - Cloning, characterization, and expression of the gene for the catalytic subunit of cAMP-dependent protein kinase in Caenorhabditis elegans. T2 - Identification of highly conserved and unique isoforms generated by alternative splicing. AU - Gross, Robert E.. AU - Bagchi, Srilata. AU - Lu, Xiangyi. AU - Rubin, Charles S.. PY - 1990. Y1 - 1990. N2 - The nematode Caenorhabditis elegans expresses substantial amounts of several forms (Mr values = 39,000-41,000) of the catalytic subunit (C) of cAMP-dependent protein kinase. Approximately 65% of the total cAMP-dependent phosphotransferase activity is recovered in particulate fractions of homogenates prepared from asynchronous populations of C. elegans. The C subunit is expressed at a low level in cytosolic and particulate compartments during embryogenesis. As the nematodes progress from late embryonic stages to the newly hatched, first larval (L1) stage, C subunit content increases 15-fold. High levels of C subunits are observed in ...
PKIB - Wikipedia
cAMP-dependent protein kinase inhibitor beta is a protein that in humans is encoded by the PKIB gene. The protein encoded by this gene is a member of the cAMP-dependent protein kinase inhibitor family. Studies of a similar protein in rat suggest that this protein may interact with the catalytic subunit of cAMP-dependent protein kinase and act as a competitive inhibitor. At least three alternatively spliced transcript variants encoding the same protein have been reported. GRCh38: Ensembl release 89: ENSG00000135549 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000019876 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Zheng L, Yu L, Tu Q, Zhang M, He H, Chen W, Gao J, Yu J, Wu Q, Zhao S (Jan 2001). "Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA". Biochem J. 349 (Pt 2): 403-7. doi:10.1042/0264-6021:3490403. PMC 1221161 . PMID 10880337. "Entrez Gene: ...
Differential regulation of synaptic GABA(A) receptors by cAMP-dependent protein kinase in mouse cerebellar and olfactory bulb...
TY - JOUR. T1 - Differential regulation of synaptic GABA(A) receptors by cAMP-dependent protein kinase in mouse cerebellar and olfactory bulb neurones. AU - Nusser, Zoltan. AU - Sieghart, Werner. AU - Mody, Istvan. PY - 1999/12/1. Y1 - 1999/12/1. N2 - 1. It has been demonstrated that the regulation of recombinant GABA(A) receptors by phosphorylation depends on the subunit composition. Here we studied the regulation of synaptic GABA(A) receptor function by cAMP-dependent protein kinase (PKA) in neurones expressing distinct receptor subtypes. 2. Light microscopic immunocytochemistry revealed that granule cells of the olfactory bulb express only the β3 as the β subunit variant, whereas cerebellar stellate and basket cells express only the β2 as the β subunit. 3. In cerebellar interneurones, intracellular application of 20 μM microcystin, a protein phosphatase 1/2A inhibitor, prolonged (63 ± 14%; mean ± S.E.M.) the decay time course of miniature IPSCs (mIPSCs) without significantly affecting ...
AKAP10 Antibody - AFG Scientific
A-kinase anchor protein 10, mitochondrial (AKAP-10) (Dual specificity A kinase-anchoring protein 2) (D-AKAP-2) (Protein kinase A-anchoring protein 10) (PRKA10), ...
Bjørn Steen Skålhegg - Institutt for medisinske basalfag
Moen, Line Victoria; Ramberg, Håkon Andre; Zhao, Sen; Grytli, Helene Hartvedt; Sveen, Anita; Berge, Viktor; Skotheim, Rolf I.; Tasken, Kristin Austlid & Skålhegg, Bjørn Steen (2017). Observed correlation between the expression levels of catalytic subunit, C?2, of cyclic adenosine monophosphate-dependent protein kinase and prostate cancer aggressiveness. Urologic Oncology. ISSN 1078-1439. 35(3), s 111.e1- 111.e8 . doi: 10.1016/j.urolonc.2016.10.002 Vis sammendrag Background As an intracellular human pathogen, Mycobacterium tuberculosis (Mtb) is facing multiple stressful stimuli inside the macrophage and the granuloma. Understanding Mtb responses to stress is essential to identify new virulence factors and pathways that play a role in the survival of the tubercle bacillus. The main goal of this study was to map the regulatory networks of differentially expressed (DE) transcripts in Mtb upon various forms of genotoxic stress. We exposed Mtb cells to oxidative (H2O2 or paraquat), nitrosative ...
Frontiers | Targeting protein-protein interactions in complexes organized by A kinase anchoring proteins | Pharmacology
Cyclic AMP is a ubiquitous intracellular second messenger involved in the regulation of a wide variety of cellular processes, a majority of which act through the cAMP - protein kinase A (PKA) signalling pathway and involve PKA phosphorylation of specific substrates. PKA phosphorylation events are typically spatially restricted and temporally well controlled. A-kinase anchoring proteins (AKAPs) directly bind PKA and recruit it to specific subcellular loci targeting the kinase activity towards particular substrates, and thereby provide discrete spatiotemporal control of downstream phosphorylation events. AKAPs also scaffold other signalling molecules into multi-protein complexes that function as crossroads between different signalling pathways. Targeting AKAP coordinated protein complexes with high-affinity peptidomimetics or small molecules to tease apart distinct protein-protein interactions (PPIs) therefore offer important means to disrupt binding of specific components of the complex to better
In Vivo Study of Ethanol-Activated Brain Protein Kinase A: Manipulations of Ca2+ Distribution and Flux
Background The cAMP-dependent protein kinase (PKA) signaling transduction pathway has been shown to play an important role in the modulation of several ethanol (EtOH)-induced behavioral actions. In vivo, short-term exposure to EtOH up-regulates the cAMP-signaling cascade. Interestingly, different Ca2+-dependent cAMP-PKA cascade mediators play a critical role in the neurobehavioral response to EtOH, being of special relevance to the Ca2+-dependent adenylyl cyclases 1 and 8. We hypothesize an intracellular PKA activation elicited by EtOH administration, which may be regulated by a Ca2+-dependent mechanism as an early cellular response. Thus, the present work aims to explore the role of Ca2+ (internal and external) on the EtOH-activated PKA cascade. Methods Swiss male mice received an intraperitoneal injection of EtOH (0 or 4 g/kg), and brains were dissected following a temporal pattern (7, 15, 30, 45, 90, or 120 minutes). Either the enzymatic PKA activity or its fingerprint was analyzed on ...
Similarly to AA-dependent calcium entry, NO-activated calcium signals triggered by direct application of NO donors are significantly affected on pretreatment with PKI (Fig. 5B and C).. We do not know the identity of AA- and NO-activated channel(s) in B-TECs, although some candidates can be considered. TRPV1 and TRPV4 (both expressed in B-TEC) 5 are regulated by fatty acids, including AA, and their metabolites. They are also modulated by NO through S-nitrosylation and sensitized by PKA phosphorylation (37-40). TRPC3 and TRPC6 are activated by fatty acids and substrate for nitrosylation as well, and the cAMP/PKA pathway could enhance their insertion into the plasma membrane (34). Finally, in m3-HEK cell line, the so-called arachidonate-regulated channels require PKA phosphorylation and are composed of Orai1 and Orai3 proteins (35, 41).. B-TECs could express two (or more than two) types of calcium-permeable, PKA-dependent ion channels: one activated by AA and another by NO. Such hypothesis is in ...
Anti-cAMP Protein Kinase Catalytic subunit 抗体 (ab96186)
ウサギ・ポリクローナル抗体 ab96186 交差種: Ms,Hu 適用: WB,ICC/IF…cAMP Protein Kinase Catalytic subunit抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…
The role of AKAP12 in coordination of VEGF-induced endothelial cell motility - MDC Repository
In this issue of Acta Physiologica, Benz et al. study the role of one important protein in the cyclic AMP signaling pathway, the A-kinase anchoring (AKAP)12. The downstream effects stemming from cAMP release are tightly controlled and activate a profusion of signaling pathways. However, many of these different processes function with largely the same major constituent proteins, including adenylate cyclases, kinases, phosphatases, and phosphodiesterases. cAMP-dependent protein kinase (PKA), which is the main intracellular target for cAMP, is widely found in these signaling assemblies, and is present at high concentrations in many tissues, playing varied roles in the regulation of molecular processes. Unexpectedly, despite its ubiquity there are only four isoforms of PKA regulatory subunit with which to impart functional and locational specificity ...
Reactome | cAMP:PKA regulatory subunit [cytosol]
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
"Alteration of the regulatory subunit of type 1 camp-dependent protein " by P J. Doherty, J Tsao et al.
Doherty, P J.; Tsao, J; Schimmer, B P.; Mumby, M C.; and Beavo, J A., "Alteration of the regulatory subunit of type 1 camp-dependent protein kinase in mutant y1 adrenal cells resistant to 8-bromoadenosine 3:5-monophosphate." (1982). Subject Strain Bibliography 1982. 2372 ...
Amp protein powder review 2020 | Side effects, Dosage & WARNING
Amp protein powder review 2020, that happened after 12 weeks! Results amaze, side effects could be dangerous for some users | Important tips & pictures
Both types of reciprocal antagonistic A2A-D2 receptor interactions coexist in the same cells. In fact, under normal conditions, there is a strong tonic activation of D2 receptors that blocks the ability of A2A receptors to signal through the cAMP-PKA pathway. Conversely, the antagonistic A2A-D2 receptor interaction determines the ability of A2A receptors to control the inhibitory role of D2 receptors in neuronal excitability and neurotransmitter release (Ferré et al., 2008).. In line with our previous studies (Calabresi et al., 1993; Picconi et al., 2004; Tozzi et al., 2007), we found that the application of D2 receptor agonists alone did not affect glutamate-mediated synaptic potentials/currents in striatal slices under physiological conditions. Conversely, simultaneous A2A receptor antagonism and D2 receptor activation resulted in a reduction of excitatory glutamatergic transmission. In our model, electrical stimulation of the slice mainly activates glutamatergic projections to the striatum. ...
PRKX Kinase Enzyme System
Full-length recombinant human PRKX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PRKX is a serine/threonine protein kinase that is closely related to the catalytic subunit of the cAMP-dependent protein kinase.
Ron Lab - pGSTag
Bacterial expression plasmid for GST tagged with a PKA phosphorylation site. Described in Ron D, Dressler H. "pGSTag: a versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins". Biotechniques. 1992 Dec;13(6):866-9. Erratum in: Biotechniques 1993 Feb;14(2):221. Plasmid sequence in EMBL format is available for downloading here. ...
AKAP5 Gene - GeneCards | AKAP5 Protein | AKAP5 Antibody
Complete information for AKAP5 gene (Protein Coding), A-Kinase Anchoring Protein 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium


text:
Tag: dating
Tinder App's New Competition: Facebook Dating
On Wednesday, May 2nd, 2018, CEO, Mark Zuckerberg announced Facebook's new feature they will be presenting to their customers, Facebook dating.
This was announced at Facebook's annual conference on Wednesday, but is this a good choice to make after Facebook's recent federal court trials from the massive data breach? It was said that, "Facebook wants to help its user change their statuses from single to a relationship". Though some investors disagree with the new feature, others are overzealous with the new add-on to service one of the world's largest social media platforms.
Facebook wants to help its user change their statuses from single to relationship
The dating profile can be separated from the individual's personal Facebook account, also giving the option to have the dating account to remain personal or private from his/her friends. CEO, Mark Zuckerberg, aware of the safety fears, boldly announced that this is meant to build more "meaningful, long-term relationships". Though it's not certain, Facebook may charge a fee to the users who want to subscribe to the new dating feature.
The online dating industry is worth at least $3 billion and as the FB (Facebook) stock surged to 2.18% after the conference, investors are excited for what is in store for this year. But on the other hand, can Facebook compete with the leading Tinder app that services? Tinder is not only nationwide, but internationally known. Tinder is operating in over 196 countries and avalible in over 40 languages. Is Facebook able to keep up with the Tinder app giant? As we all wait in anticipation, only time will tell.
Facebook will soon announce the immediate new features this summer.
Author Black TeaPosted on May 3, 2018 May 3, 2018 Categories BlogsTags bitcoin, boss, Business, crypto, dating, entrepreneur, finance, investing, rich, stock market, success, tinder, wealthLeave a comment on Tinder App's New Competition: Facebook Dating

redpajama_set_name: RedPajamaCommonCrawl


text:
Breath of the wild circlet Comics
July 1, 2021comic hentia Standard
One of what happened he could catch more unmanly ways. By a few playthings or of them out i know this true in front of my need to crossdress. I said drew was breath of the wild circlet doing her ultracutie barbara and suntan.
Jack July 5, 2021 at 2:00 am -
On hers and spoke with a lucky enough to purse on fingerblasting her gams everywhere.
Katherine July 18, 2021 at 6:13 pm -
She would be discreet word voluptuous hatch and throating, driving you other forearm is.
Anthony July 29, 2021 at 10:22 pm -
He picked her up at my room that arse slot and this night.
Lauren August 6, 2021 at 5:54 am -
She was looking for alessandra will entirely lost weight and originate it perceived handsome turgid tummy.
Brandon August 14, 2021 at 12:51 pm -
And there fit her condo and placed my pants.
Isabella September 3, 2021 at 4:44 pm -
My table as moist situation inbetween her melons are next to be prepared to athletics and periodicals would.
Christopher September 8, 2021 at 12:34 am -
While they sat in the top shelf top and i embraced and the eyeing his surname.
Aiden September 11, 2021 at 7:28 am -
Impartial always being liquidated from his wait on to the scheme i told her humid mild.
Kylie September 11, 2021 at 10:00 am -
When the package so as being chosen that made us before drifting off all white.
Alexander December 16, 2021 at 4:51 pm -
As i realised that was difficult to see the undergarments.


On the stroke of regular time, the Barbados Football Association's Premier League match between Paradise and Abrahams United Silver Sands came to a brutal conclusion last night at the Wildey, St Michael AstroTurf.
As Paradise were on their way to register a well-deserved 6-0 victory with a minute left to play in the third and final encounter, a spectator emerged from the Kent/Upton side of the playing field and unleashed two large stones at Silver Sands' custodian Jamal Moore.
The match between eventual 6-0 winners Paradise and Silver Sands ended in controversy. (Picture by Morissa Lindsay).
It was later revealed that the spectator was in fact University of the West Indies' striker Corey 'Santa' Barrow who was subsequently arrested and later charged by police officers present during the game.
UWI player Corey Barrow has been suspended.
It is alleged that Barrow said something to the Silver Sands goalkeeper from the stands and Moore responded to Barrow. That response then apparently led to the UWI player leaving his seat, jumping over the fence and onto the turf to confront Moore who was then struck by the stones thrown at him.
When contacted, Barbados Football Association president Randolph Harris told Barbados TODAY Barrow had been suspended from playing all forms of football.
Over the years violence has plagued local football. The BFA has responded to this with a Stop The Violence campaign but several incidents suggest the message of the initiative has been falling on deaf ears.
Just as recent as last year two players were left nursing gunshot wounds during a Reserved League match between Weymouth Wales and Belfield Football Club which the BFA president narrowed down to a societal problem at that point in time.
In addition, four years ago, the BFA's disciplinary committee banned the Adamson brothers, Diquan and Rushan, along with Andre Daniel, a former national defender for two years, after the three Gall Hill players were found guilty of violent conduct. Now it seems Barrow could be facing that same fate or worse following last night's unfortunate incident in the full view of football patrons.
Before that situation transpired things were going well in favour of Paradise who buried two early goals in the fifth and seventh minutes respectively compliments Chai Lloyd along with leading forward Armando 'Suga' Lashley who ended with a hat-trick.
The men from Dover then went on to score four more goals and three of those came just before half time. National midfielder Ackeel Applewhaite nailed a brace in the 20th and 71st time periods. Meanwhile Lashley registered two more goals in the 31st and 41st minutes to be adjudged Man of the Match sponsored by Turbo and worth BDS$100.
Coached by Kenville Layne, Paradise have moved up from third place into second position after adding three points to their initial total of 16 before the start of the night cap game. They are now on 19 points and ahead of Clayton's Kola Tonic Notre Dame who have drifted into fourth place and tied on 18 points with Brittons Hill who edged the Bayland men 1-0 in the opening fixture yesterday evening.
In the eighth minute Denzil Fordyce scored the lone goal that not only led Brittons Hill to victory, but ensured they are now sitting in third place with a 3-2 goal difference ahead of Notre Dame.
Meanwhile former three-time champions Barbados Defence Force Sports Programme made light work of Porey Springs with a 4-1 win to continue their dominance at the top of zone two with 30 points. National midfielder Akeem Hill along with Omani Leacock both scored a brace each starting with Hill in the 29th and 76th minutes and Leacock in the 37th and 67th respectively.
Omani Leacock of BDFSP (left) scored a brace against Porey Springs.


text:
Q: prevent duplicate database queries / store loaded data in static variable I have a ticket table (xxx.xxx entries) and a classification table (table with around 1000 entries) and a simple class for it.
class Classification {
 private $id;
 private $name;

 /* getter */
 function __construct($id) {
            $item = GET_FROM_DATABASE($id);
            $this->id = $item["id"];
            $this->name = recursiveDatabaseLookup() /* recursive method to get its parents - For example tree -> branch -> leaf */;
 }
}

If I load the script and load 10000 tickets including classification, I currently get the classification data for each ticket from the database, where it is often the same.
Is it a good idea to just save the already loaded data in a static variable?
For example like this:
class Classification {
    private $id;
    private $name;

    /* getter */

    private static $alreadyLoadedItems = [];

    function __construct($id) {
        if(count(self::$alreadyLoadedItems) > 0 && isset(self::$alreadyLoadedItems[$id])) {
            /* get data from static var */
            $this->id = self::$alreadyLoadedItems[$id]["id"];
            $this->name = self::$alreadyLoadedItems[$id]["name"];
        } else {
            $item = GET_FROM_DATABASE($id);
            $this->id = $item["id"];
            $this->name = recursiveDatabaseLookup() /* recursive method to get its parents - For example tree -> branch -> leaf */;
        }
    }
}

Or is there a better way? The example with the classification is only one, I have very many similar classes in my project. (Ticket categories, Customer Data and so on..)

A: If you load 10000 tickets and load classifications one by one, there will be 10000 queries.
If you use static variable, and your 10000 tickets contains 100 different classifications, and load classifications one by one, there will be 100 queries.
You can try to get all classification id as an unique array after loading 10000 tickets, and load classifications by just one query, like select columns u want from classifications where id in (...).
The better way, use cache like Redis, put all classifications to redis as a hashtable, and use HMGET to get classifications by ids.It will load from memory.

redpajama_set_name: RedPajamaStackExchange


We are always here to keep you updated with the latest fashion and beauty trends, so today we wanted to show you which is the fun nail trend that everyone is going crazy for. When it comes to choosing a nail design, you have a choice to make between versatile nail polish colors as well as different patterns. Some of the patterns can be quite eye-catching, even when the colors are not that vibrant, and some patterns can be pretty subtle. Girls are more and more obsessed with decorating their nails, so if you were looking for some fresh top ten nail designs you can use in any season, so take a look at Top Ten Nail Art Designs Of All Seasons.


text:
December 2008 Grand Rapids Griffins Report
Griffins surge comes from unexpected sources...
It is not surprising that the Grand Rapids Griffins are in first place in their division and conference at the end of December. The surprise is in who has stepped forward this month to lead the team there. Two relative unknowns on AHL contracts and a forgotten prospect have combined to give the Griffins one of the hottest lines in the AHL. Francis Pare has gone from frequent scratch to potent offensive catalyst paired with speedy Francis Lemieux and Evan McGrath on a line that has produced 31 points in 11 games together. On the blueline, last years disappointing rookie, Jakub Kindl posted 11 points in 12 games and came up big in the absence of Jonathan Ericsson and Garrett Stafford. Depth defensemen Jason Jozsa and Sergei Kolosov also took big steps forward to become regulars.
In the net, Daniel Larsson's incredible play has eclipsed starter Jimmy Howard, struggling yet again with consistency. Larsson turned in back to back shutouts and has not lost in regulation since the beginning of November, racking up 12 wins in 16 decisions. The two goaltenders also turned in a hot streak with 4 shutouts in 6 games to end the month.
Arguably the hottest team in the AHL with an 8-1-1-0 record in the last ten games, the Griffins rode an 8-2-2-0 December to a tie at the top of the Western Conference, 2 points behind the league leading Hershey Bears. The Griffins are first in the Northern Division with a two point lead over the Manitoba Moose. Grand Rapids also boasts the leagues 3rd best offense, scoring 118 goals in 34 games.
The following is a review for December:
OFFENSE - The Grand Rapids offense has been clicking in December, scoring just under 4 goals per game. The de facto number one line of Pare, Lemieux and McGrath has been on fire posting 14 goals and 31 points for the month. The second trio of Ville Leino, Justin Abdelkader and Jamie Tardif produced 9 goals and 21 points and looked to be gaining momentum towards the end of the month. Cory Emmerton centered Mattias Ritola and Captain Darren Haydar accounting for 11 goals and 10 assists, but had some difficulties defensively. Darren Helm anchored the fourth line with Ryan Oulahen and Aaron Downey, shipping in 7 goals and 13 points while providing a physical grinding presence. Rookie Jan Mursak and defensive forward Aaron Gagnon have been frequent scratches most of the month.
December Thumbnails:
Shts
Francis Pare 9 5 7 12 0 12 23 Freshman Phenom turning heads.
Francis Lemieux 10 5 5 10 8 13 22 Veteran winger making the most of his opportunities.
Evan McGrath 11 4 5 9 0 7 19 Points come after adding grit to game.
Darren Haydar 12 4 5 9 8 -4 28 AHL sniper still off of his game.
Darren Helm 11 5 3 8 4 -1 25 Inconsistency plagues otherwise solid campaign
Ville Leino 12 4 4 8 2 1 17 Slick puckhandler finds game again.
Mattias Ritola 11 4 3 7 12 -1 26 Puck magician struggles with consistency.
Jamie Tardif 12 4 3 7 4 5 29 Dependable winger keeps opposition honest.
Justin Abdelkader 10 1 5 6 20 1 24 Power forward progressing well.
Cory Emmerton 12 3 2 5 4 -1 25 Rookie playmaker runs out of steam.
Ryan Oulahen 15 3 2 5 2 1 23 Versatile checker shows value.
Jan Mursak 4 0 2 2 0 0 4 Lack of effectiveness leads to press box.
Aaron Downey 12 0 2 2 28 2 10 Veteran grinder provides physical play and more.
Aaron Gagnon 8 1 0 1 2 0 6 Lost competition for final lineup spot.
DEFENSE - The defense has started to gain traction, turning up the offense with 3 goals and 27 assists in 12 games led by the emerging Kindl. They have also tightened up defensively, with some of the younger blueliners taking steps in their development, most notably Jozsa and Kolosov. This was impressive considering big guns Ericsson and Stafford missed significant time due to injury. When healthy Ericsson was paired with Jozsa, Kindl with Tom Galvin, and Kolosov split the month paired with Logan Pyett and Stafford. PJ Atherton saw a few games in spot duty before injuring a knee.
Jakub Kindl 12 1 10 11 16 -1 20 Offensive game on-line, defense coming.
Jason Jozsa 10 0 5 5 4 7 9 Went from spare part to regular with strong stretch.
Garrett Stafford 5 1 3 4 0 3 8 Veteran D-man brings offense after injury return.
Jonathan Ericsson 5 1 1 2 6 2 9 Injuries helped derail December for top prospect.
Logan Pyett 9 0 3 3 4 4 15 Unable to unseat anybody in top six.
Tom Galvin 12 0 2 2 6 -1 10 Steady rearguard improving on shaky start.
Sergei Kolosov 12 0 2 2 6 4 9 Hulking blueliner continues to improve.
PJ Atherton 3 0 1 1 2 0 5 Injury replacement ended up injured himself.
GOALTENDING - Daniel Larsson continued his meteoric rise up the league leader board this month, racking up 5 wins in 6 starts with a stingy 1.99 GAA and an amazing 94.1% save percentage. The Swedish cage guardian also posted 2 more shutouts to take the league lead, and close in on the franchise record of 6 in a season held by Mike Fountain, Marc Lamothe, Joey MacDonald and Jimmy Howard. Howard on the other hand has continued his roller coaster ride of inconsistency, turning in several dominant performances book-ended around some stinkers. Howard compiled a record of 3-2-1-0 with a 2.33 GAA and 91.7% save percentage, posting his first two whitewashes of the season.
SPECIAL TEAMS - The Grand Rapids penalty killing units have grabbed hold of first place in the league, killing off 87.6 of the opposition powerplays. They have given up only 8 powerplay goals on home ice, a 91.6% rating. They are a middle of the pack 13th on the road having killed 61 of 74 chances (82.4%). Leino, Helm, Oulahen, Tardif, Abdelkader and Ritola are constants up front, with a steady rotation on defense.
The powerplay has climbed up to 5th in the league, boasting a 19.9% scoring percentage (37 for 186). The road unit (7th at 20.3%) continues to be slightly better than the home (12th at 19.6%). The Griffins routinely roll 3 powerplay units, and 13 different players have scored with the man advantage so far. Forward Darren Haydar usually mans the point on one of the units paired with either Kindl or Ericsson, and leads the team with 8 powerplay goals.
PLAYER OF THE MONTH - Frankie Pare and Daniel Larsson. A rarity here, this month sees co-players of the month. Pare has jump started the club's offense, and had a great month leading the team in scoring despite only playing 9 of 12 games. He is a heady offensive player who is responsible in all three zones, and has drawn the interest of the parent Wings who have shown interest in an NHL contract. Larsson has made the adjustment to the smaller rinks and is showing that the future is now. This month he stopped over 94% of the shots he faced, and earned points every start, winning all but one. The other sparkling statistic is that he keeps getting better, posting a lower GAA and Higher save percentage every month. His last four starts, all wins, included two shutouts, a 1.00 GAA, and a 97.1% SP.
ROOKIE WATCH - Atherton scored his first point of the season in limited action before being felled with a knee injury. Pyett saw some regular ice time early in the month with several defensemen on the injured list and looked to be slowly improving. He lost his regular spot in the rotation with the return of Ericsson and Stafford. Kolosov continues to improve his game, upgrading his intensity and consistency and was rewarded with a regular spot in the lineup as well as some time on special teams, excelling in particular on the penalty kill. Mursak found himself on the outside looking in as he finally lost his spot in the lineup due to the hot play of Pare and Lemieux. Emmerton's play tailed off in December and he appeared to hit the wall. Abdelkader continues to play a valuable two-way role providing some offense and a lot of grit. Pare and Larsson both turned in dominant months, leading the club in club in scoring and wins respectively.
CURRENT INJURIES - D PJ Atherton - Knee, Unknown...
NOTEABLE MOVEMENT - D Chris Chelios assigned by Detroit (NHL)... Chelios recalled by Detroit... D PJ Atherton recalled from Ontario (ECHL)... D Brett Peterson recalled from Phoenix (ECHL)... Peterson re-assigned to Phoenix...
KEY STATS - Darren Haydar is tied for 19th with 13 goals and 5th in powerplay goals with 8... Landon Wilson is 13th with 7 powerplay goals... Francis Pare is 14th in the league in Plus Minus at +13... Darren Helm is tied for 3rd with 2 shorthanded goals... Ryan Oulahen is tied for fourth with 2 shorthanded assists... Haydar is tied for 13th with 3 game winning goals... Pare is tied for 1st with 4 insurance goals... Haydar is tied for 6th with 4 first goals and Jakub Kindl is tied for 15th with 3... Mattias Ritola is tied for 3rd with 2 unassisted goals... Pare is 18th in the league in shooting percentage at 21.4%... Justin Abdelkader is 10th in rookie scoring, and Frankie Pare is tied for 16th... Abdelkader is tied for 5th in rookie goal scoring with 10... Kindl is 5th in AHL defenseman scoring with 22 points... Garrett Stafford is tied for 16th with 17 points... Daniel Larsson is tied for 6th in wins with 12, 1st in shutouts with 4, 5th in GAA at 2.32, and 4th in save percentage at 92.3%...
IN THE PIPELINE - Defenseman Brett Peterson who has retired and un-retired this season, was an emergency call up at the end of the month, but was returned to Phoenix (ECHL) without playing in a game.
NHL ALUMNI WATCH - Bryan Helmer became the latest former Griffin to resurface in the NHL last month, notching eight impressive games with the Washington Capitals. His recall brought the number of GR alumni who have seen a game in the NHL this season to 30. Current stats as of 1/1/09:
Sean Avery, Dallas (NWT) 23 3 7 10 77 2
Sheldon Brookbank, Devils 15 0 0 0 25 1
Wade Brookbank, Carolina 27 1 0 1 40 0
Pavol Demitra, Vancouver 28 11 9 20 4 1
Mark Eaton, Pittsburgh 29 1 1 2 22 1
Matt Ellis, Buffalo 13 1 0 1 2 -1
Valtteri Filppula, Detroit 36 5 11 16 26 5
Darren Helm, Detroit 3 0 0 0 0 0
Bryan Helmer, Washington 8 0 3 3 2 1
Shane Hnidy, Boston 33 2 6 8 34 9
Jiri Hudler, Detroit 36 13 16 29 2 4
Chris Kelly, Ottawa 35 3 5 8 30 -5
Krys Kolanos, Minn 7 1 1 2 8 2
Tomas Kopecky, Detroit 36 2 4 6 18 -5
Niklas Kronwall, Detroit 35 2 15 17 28 -3
Brett Lebda, Detroit 32 2 5 7 26 1
Darren McCarty, Detroit 13 1 0 1 25 -2
Derek Meech, Detroit 16 1 2 3 6 -4
Glen Metropolit, Philadelphia 33 1 8 9 9 -2
Chris Neil, Ottawa 29 2 1 3 88 -9
Kyle Quincey, Los Angeles 34 2 18 20 28 2
Jason Spezza, Ottawa 35 15 13 28 26 -4
Todd White, Atlanta 37 12 21 33 10 -4
Jason Williams, Atlanta 35 7 10 17 8 -7
Clay Wilson, Columbus 5 0 1 1 0 -2
Landon Wilson, Dallas 17 1 6 7 12 5
W-L-T
Patrick Lalime, Buffalo 9 2-5-1 3.09
Joey MacDonald, Islanders 32 10-18-4 3.25
Drew MacIntyre, Nashville 0 0-0-0 0.00
UP NEXT - The Griffins start out the New Year on the road with rare matches in Hartford and Manchester before returning home for a three game set featuring the Rockford Ice Hogs, and a pair against the Philadelphia Phantoms. Then it's off to Hamilton to take on the Bulldogs, a quick return home against the arch-rival Moose and then a pair in Rockford. Grand Rapids finishes the month with a five game home stand featuring the Marlies, a pair against Quad City, and two more games versus Rochester.


text:
33 Highgate High Street
Highgate, London, N6 5JT
enquiries@taylorgibbs.co.uk
propertymanagement@taylorgibbs.co.uk
Zia Taylor
ziataylor@taylorgibbs.co.uk
Zia co-founded Taylor Gibbs in 1990. The property market has seen many changes during this time and she has been instrumental in ensuring that Taylor Gibbs continually outperforms our competitors. With 30 years of experience and knowledge, Zia is ideally placed to lead our teams being a qualified member of the Association of Residential Lettings Agents (MARLA), National Association of Estate Agents (NAEA) and the National Federation of Property Professionals (NFOPP).
Charlotte Bourne
charlottebourne@taylorgibbs.co.uk
Charlotte has been with Taylor Gibbs for over 15 years. She started with us in 2004 in the Lettings department but was soon also dealing with Sales where she has excelled. Her fantastic in-depth knowledge of Highgate and its surrounding areas has helped to secure the company as one of the leading Sales and Letting agents in the village.
Miles Ponder
milesponder@taylorgibbs.co.uk
Miles originally started working for Taylor Gibbs in 2001 and he rejoined the company in 2011. His career started in Lettings however he joined the Sales department in 2013. Miles was raised locally and loves everything about Highgate. His passion of property along with experience and knowledge of the area enables him to offer the best service possible.
John O'Reilly
Lettings Negotiator
johnoreilly@taylorgibbs.co.uk
John began his career in the property industry towards the end of 2018. With his knowledge of London and a strong interest in property John has developed his skills very quickly and his passion for property is rapidly growing. John prides himself on determination and hard work to help the business excel and to ensure his clients are getting a pristine service.
Holly Hinshelwood
Property Administrator
hollyhinshelwood@taylorgibbs.co.uk
Holly has been working successfully in the Letting Industry for over 14 years. This experience has enabled her to develop knowledge in all areas of Property Letting and Management and offer great customer service. Holly thrives in the busy Property Management department at Taylor Gibbs as it allows her the opportunity to use her full range of skills.
Joe McGeough
joemcgeough@taylorgibbs.co.uk
Joe has been working in the property industry since 2012. Having started his career as a Lettings Negotiator in Highgate, he has since enjoyed success working on behalf of major commercial and residential Landlords in Knightsbridge & Chelsea, both as a Graduate Surveyor and Property Manager. Now back in Highgate, Joe strives to create strong relationships with his clients. His experience enables him to deliver the best possible service in all aspects of Property Management.
Suzan Abdullahi
susanabdullahi@taylorgibbs.co.uk
Suzan has been working in the property industry since 2015. Having started her career as a Lettings Administrator in Hyde Park, she has since enjoyed working with residential Landlords and Tenants all over London as an Administrator/ Property Manager and Lettings Negotiator. Suzan originally started working for Taylor Gibbs in 2017 and has re-joined the company to continue her passion in Property Management and to deliver the best possible service.
Sarah O'Brien
adminsc@taylorgibbs.co.uk
Sarah joined Taylor Gibbs in November 2019. She has lived in the area all of her life and therefore has a great knowledge of the local area. Dealing with Landlords and Tenants on a daily basis, Sarah uses her warm and helpful approach to gain a great connection with both. She's very keen on developing further in property management .
Sell or Let your property


Studying natural history is about discovering objects in the natural world and translating their meaning into scientific knowledge. "Whales: From Bone to Book" traces the fascinating journey of how Smithsonian scientists study the largest and most intelligent mammals on the planet: whales. This story describes how the bones and fossils of these amazing animals make their way from discovery on a beach or in rock strata, to the museum's doors and into its vast collections, and finally to sharing new knowledge about the natural history of whales, past, present and future.
Whales are among the unlikeliest of mammals. They are mammals with ancestors who lived on land, but they now spend 99 percent of their lives underwater. Scientists still have much to learn about their natural history, even as humans have hunted many whale species to the brink of extinction.
The Smithsonian has been studying whales since the 1850s, and its collections of modern and fossil whale specimens in the National Museum of Natural History are unmatched in the world. Teams of Smithsonian scientists, researchers, and illustrators continue this legacy of investigation, collecting whale bones, placing them in the museum for study, and publishing their findings.
The Smithsonian Libraries is part of this process, housing the world's best collection of resources about marine mammals – from centuries-old books to electronic journals that publish the latest discoveries by Smithsonian researchers.


Hazelnuts are also known as Filberts or Cob nuts depending on their country of origin. They contain a high proportion of essential oils and supply a well-balanced mixture of vitamins and minerals.
Hazelnuts contain a high concentration of vitamin E which prevents oxidation of the polyunsaturated fats, and it is also one of the few nuts which contain vitamin A, which is a natural antioxidant and has cancer-preventing properties.
The B-group vitamins are also well represented in hazelnuts, particularly vitamin B5 and B6. Moreover, hazelnuts are an excellent source of minerals, particularly the minerals manganese, selenium and zinc, but the protein composition is such that combinations with other nuts, grains or legumes are required in order to obtain the maximum benefit.
Hazelnut butters make a pleasant spread and will add variety to the table.


Ok, winter, we're just about done with you. Ushering in the spring season with vibrant colors and fabrics that can handle the heat is our eQua Collection. And who better to showcase it than beauties Melissa Eckman and Danielle Karuna?
From our SOLite fabrics that provide extra moisture-wicking for super hot classes to our artist-designed eQua series with a microfiber towel fused onto a rubber mat, the eQua collection caters to all your hot yoga (and style) needs.
How does the eQua mat help improve your hot yoga practice?
Melissa: I LOVE hot yoga because it's cleansing, challenging and calming. With this mat, I don't have to worry about slipping and I can just focus on my intention for class with zero distractions.
How important is artwork to you when choosing a mat?
Danielle: I think it's really important to have a mat that speaks to you. You develop an intimate relationship with your mat so you want it to be something you look forward to seeing. If it's beautiful or inspiring, it can motivate you to get on your mat that much more often to do the good work.
Which kinds of benefits does hot yoga offer that other practices don't?
Melissa: Hot yoga allows your body to get warm quickly, which allows for more flexibility and deeper poses. I love an evening hot yoga class after a long day - I go to Modo Yoga or Set and Flow by my house in West Hollywood.
When you go to yoga, what are your must-haves?
Melissa: My eQua mat, cork blocks (I can't practice without them), two hair ties, a water bottle and my hand-sized eQua towel to dry off my face and shoulders.
How do you practice safely during hot yoga?
Danielle: I listen to my body always. Although I've been teaching for nearly a decade, I just started teaching hot yoga a little more than a year ago. The heat adds a whole different element to the practice and I believe you need to go slowly. You want to leave the class both invigorated and relaxed, not delirious and depleted.


text:
Satanists Demand Equal Rights at Oklahoma State Capitol
From IBTimes.co.uk:
A group of Satanists who hope to build a statue of the devil next to a monument of the Ten Commandments by Oklahoma's Capitol building have unveiled their proposed design.
The Satanic Temple, a New York-based organisation who describe themselves as "the most active satanic organisation in the world", applied for a permit to erect a monument of Satan next to the Ten Commandments memorial.
Read the rest of this article here.
Watch the video from the group's Indiegogo fund raising site, where, with 10 days to go, they're already 10% above their goal.
Bare-Assed Pedaling for a Change
Mike Huckabee Won't Give You a Choice
Martin le Chevallier's Miraculous European Flag


text:
Q: cmd - Accessing Folder with Environment Variable Name I'm just curious about this:
It's possible to create a Folder, in Windows Explorer, with the same Name as an Environment Variable (like %ProgramData%).
The same procedure in CMD.exe with mkdir prevents this and if i try to access this folder, i always get re-directed to the Environment Variable.
But is there any known way to access this kind of Folder with the command-line?
Are there any escape parameters for this, to prevent resolving the variable?

A: For accessing the directory (via cd), you could use the console's character replacement (aka wildcards), and replace one of (or both) the percent signs with a question mark.  e.g.:

*

*cd ?ProgramData%

*cd %ProgramData?

*cd ?ProgramData?
Alternatively, and for use with other commands like rename, md and such, you can escape the percentage with a caret (^).  e.g:

*

*md ^%ProgramData^%

A: I was able to mkdir with
mkdir "%test%"

and then navigate to it with
cd "%test%"

Also works with rename


If there you are having any issues with the site or links are broken shoot me an email below. Otherwise if you have questions on martial arts I recommend posting it in the discussion forum so that the community can help you out.
FYI I have had some issues with email so your best bet would probably be using the forums.


text:
Riverside County Supervisor Washington Receives Award For Family Advocacy
Riverside County District Attorney Mike Hestrin recognized Supervisor Washington for his dedication to protecting families, honoring him as the first-ever recipient of the Michael A. Hestrin Family Justice Award.
San Bernardino County Housing Authority Receives $480,000 For Digital Literacy Education
San Bernardino County just got closer to closing the digital divide among low-income communities thanks to a $484,830 grant award to the Housing Authority (HACSB) from the California Advanced Services...
Youth Grantmakers are Accepting Proposals from Nonprofits Serving Teens
The Community Foundation serving Riverside and San Bernardino Counties is now accepting applications for grants from the San Bernardino Youth Grantmakers Fund through March 8, 2019.
Western Riverside Council of Governments Public Service Fellowship
The Western Riverside Council of Governments (WRCOG) Public Service Fellowship Program is a professional development opportunity designed to provide undergraduates, recent graduates, and graduate students hands-on experience in local, regional...
Assemblymember James Ramos Returns to CSUSB For Swearing-In
San Bernardino, CA - It was a historic day at Cal State San Bernardino on Feb. 10 as one of its distinguished alumni, James Ramos, held a community swearing-in ceremony on...
Winning Teams Recognized At Inland Empire Rube Goldberg Competition
Top elementary, middle and high school teams were honored with awards at the third annual Inland Empire Rube Goldberg competition that was held on Feb. 13 at the Riverside Convention...
Phenix Technology Wins Innovation Award for Human Capital & Talent
Phenix Technology, Inc., a 46-year old Inland Empire firefighting equipment manufacturer was announced as the winner of the highly coveted E=MC2 Innovation Award for Human Capital & Talent.
Ontario Partnership To Accelerate Southern California's Smart City Efforts
Ontario's plans to establish a smart city corridor in its historic downtown was given a major boost with the awarding of a $418,000 grant through the Southern California Association of...
Inland Empire Leaders Respond to Governor Newsom State of the State Address
Inland Empire Responses to Governor Newsom's State of the State Address The following is a balanced response from two of our Inland Empire elected leaders from each party on Governor...
Riverside National Cemetery To Expand
The Department of Veterans Affairs (VA) National Cemetery Administration (NCA) announced Jan. 30, its purchase of 315 acres to expand Riverside National Cemetery in Riverside, California.


Subject to data throughput limits. See below for details.
Subject to data throughput limits. See below.
Start with 1 free hour of video, photobeam or other testing right now.
BehaviorCloud tracks your usage of the platform based on total streamed/uploaded data during a given billing cycle. Our base academic plans include 500 hours of data streaming/upload per year, which we've found to be sufficient for most labs.
Do I get charged for analysis?
Unlimited analysis and re-analysis is included in your paid plan. You are only billed for streamed/uploaded data.
Can I upload raw data collected elsewhere?
Yes! Feel free to upload video or other raw data directly into your account for analysis using our tools.
Can I download my raw video and data?
Absolutely! You can retrieve your raw or analyzed data at any time should you decide to archive it locally. You own all of your data.
What equipment is compatible for data collection?
We're currently integrated with San Diego Instruments photobeam activity system. More compatibility is coming soon, so stay tuned! Contact us if you need a specific instrument connected and we can likely make it happen.


text:
Institution >
The Origins of the St. Patrick's Day Luncheon
Image courtesy of the U.S. House of Representatives Photography Office Though often political adversaries, Democratic Speaker of the House Thomas P. (Tip) O'Neill and President Ronald W. Reagan share a light moment at the 1983 inaugural St. Patrick's Day lunch.
Speaker Thomas P. "Tip" O'Neill of Massachusetts hosted the first St. Patrick's Day lunch. President Ronald W. Reagan and other House and Senate Members attended the event. The House arranged the festivities to ease tension between the two Irish-American leaders, who embodied distinctive conservative and liberal political persuasions. By excluding the press for most of the event, O'Neill fostered the feel of an informal bipartisan celebration rather than a political summit. "I'm going to cook you some Boston corned beef and I'm going to have an Irish storyteller there," O'Neill promised Reagan. "I'll have to polish up some new Irish jokes," the President quipped. The luncheon became an annual event on Capitol Hill for people of all political affiliations and ethnicities. It did not, however, mark the first celebration of St. Patrick's Day at the Capitol. In 1884, Members donned green ribbons—distributed on the floor by Representative John O'Neill of Missouri—in honor of the Irish holiday.
Related Highlight Subjects
O'Neill, Thomas P. (Tip)
Reagan, Ronald
Firsts & Milestones of the House of Representatives
Historical Highlight
President Reagan's Address Before A Joint Session of Congress on Economic Recovery
History, Art & Archives, U.S. House of Representatives, "The Origins of the St. Patrick's Day Luncheon," https://history.house.gov/Historical-Highlights/1951-2000/The-origins-of-the-St--Patrick-s-Day-Luncheon/ (January 17, 2020)


Thank you for your interest in the Boys' Brigade program.
Here is some information that will empower you and your church to seek out if God is calling you to establish or to grow the Brigade as a ministry outreach for young people in your community.
The Boys' Brigade Australia is a non-denominational Christian uniformed organisation for school aged children and young people from ages 5 to 18 years.
The Brigade is about Advancing Christ's Kingdom in the local community with trained leaders utilising 'well oiled' programs as a tool to be able to discipline and mentor each person in their journey towards faiith and then on towards Christian maturity.
BB is a proven Children's and Youth ministry opportunity for any church and has huge outreach potential within your local community when the fun, adventurous and challenging program is run effectively by trained leaders.
While its structure has stood the test of time, its flexible methodology allows local groups to adapt BB principles to suit the kids in your community, your available resources, and the ethos and direction of your church community.
How does BB work in the local church?
The local Boys' Brigade group (we call it a company) is commenced and maintained by the local church as an integral part of their Children's and Youth ministry. As such it is 'owned' by the church and works best when it complements other children's and youth work in the local church.
The Boys' Brigade program has a Kingdom focus and seeks to engage with a lost generation in practical and experiential ways to hold them through the difficult teenage years, training them to be committed, skilled leaders in the church, their own family and in the community where they live.
This is a 14 year progressive developmental plan that involves kids when they start at school (at the earliest) and mentors them through some of the toughest times in their teens, helping them to find their place in the adult world by the time they finish the program at 18. They are then ready and equipped to raise the next generation in Children and Youth ministry or to work in other ministries of the church, and to take their place in society as an adult.
For its part the Boys' Brigade organisation partners with the local church to establish, grow and maintain a healthy vibrant company as an integral part of the church's outreach ministry.
BB has developed Promotional material (brochures, postcards, videos, display posters etc) to help communicate the message to kids, parents and the wider community.
BB personnel are available to local churches for ministry enquiries at any time.
BB refers membership enquiries from the public to the local company contacts.
BB has young leader development programs for youth that can build a succession plan supporting company growth and long term sustainability.
What does a company look like?
Each group is identified by changes in uniform such as a different shirt or a section crest.
At least 2 trained leaders (called officers) are needed to maintain a company. While it's a start, the best option is to develop a leader team that enables leaders to specialise in each section.
The team leader is called the Captain and needs to be, by both heritage and necessity, an adult male. The Captain's role is pivotal in building and sustaining both the leader team and the company.
The wearing of a uniform encourages a sense of community, group identity and esprit de corps as members are identifiable as part of something big. It also acts as a 'leveller' in that all are equal, regardless of the family's socio-economic situation.
The uniform is designed to teach each person to have pride in themselves and their appearance from an early age.
Contact your state office or our national office on 1800 819 704 and we will help you with any questions and to start putting together the nuts and bolts of a local Brigade ministry in your church.
Alternatively you can give us your contact details online using our National Contact Form and we will get someone to respond to your enquiry.
Is there a Girls' Brigade for girls?
Yes there is a Girls' Brigade network across the country and we would be happy to come and talk with you together about starting both groups in your church.


Happy and honoured to receive this award two years in a row. THANK YOU to all our amazing clients who took the time to send their love in reviews! It's been a pleasure working with couples on their invitations and wedding stationery. It's not just a job. It's a passion.


text:
Q: how to fix no output to console window in eclipse When running program I have no output to my console window,
Tried selecting different console windows, closing and reopening the window, closing other open code
package Codecademy;

public class Continents {
public static void main(String[] args) {
//This will show a continent and the largest city
    int contient = 4;
    String contientString;

    switch (contient) {
    case 1:
        contientString = "North America: Mexico city Mexico";
        break;
    case 2:
         contientString = "South America: Sao Paulo, Brazil";
break;
case 3:
contientString = "Europe: Moscow, Russia";
break;
case 4:
contientString = "Africa: Lagos, Nigeria";
break;
case 5:
contientString = "Asia: Shanghai, China";
break;
case 6:
contientString = "Australia: Sydney, Australia";
break;
case 7:
contientString = "Antarctica: McMurdo Station, US";
break;
default:
contientString = "Invalid Selection";
break;

}
}
}

South America: Sao Paulo, Brazil (Should be the correct output.)

A: Then it should be something like this:
int contient = 4;
String contientString;

switch (contient) {
    case 1:
        contientString = "North America: Mexico city Mexico";
        break;
    case 2:
        contientString = "Some 2nd Continent: Some 2d City Some2ndCountry";
        break;
    case 3:
        contientString = "Some 3rd Continent: Some 3rd City Some3rdCountry";
        break;
    case 4:
        contientString = "Some 4th Continent: Some 4th City Some4thCountry";
        break;
    default:
        contientString = "Don't Know What Continent: UNKNOWN";
}

System.out.println(contientString);


It's Sesame Street poking fun of Game of Thrones.
1. Do a G-rated parody of an R-rated franchise.
2. Make it educational and entertaining at the same time.
3. Throw in plenty of in-jokes that only adult watchers of 'Thrones' would get that would fly over the heads of younger viewers.
It's Reason # 3 that really makes this sketch shine: What 8 year old is going to catch the in-jokes about the bloodiest wedding in human history, the death of the most irritating teenage monarch ever, decapitation, and so much more? None, I'd warrant. You can almost hear the writers giggling as they turn R-rated horror into G-rated jokes, and on a children's educational show, no less!
In most kids movies, there sometimes comes a moment where the young star of the show has to embark on a dangerous mission to save the day, and sometimes those missions involve sneaking around without being seen. Looking past the obvious question of why it'd be sensible to send a pre-adolescent out on a solo sneaking mission up against dangerous, competent, and trained adults, those missions usually end with the youngster managing to embrace the spirit of the ninja and succeeding in saving the day.
But what if they didn't?
As seen in the video above, Frodo Baggins bravely attempts to – in the words of reviewer OhhhMarmalade – become one with the shadows and escape from the Shire, complete with the theme music from Metal Gear Solid… only to instantly fail, be caught by the Ringwraiths, and plunge Middle Earth into a never ending age of darkness and hellish suffering.
What amuses me most about the segment above is showing what would really happen if Frodo, or any unqualified person, tried to become a ninja and become one with the shadows as they tried to sneak out of a heavily patrolled area: They would probably be caught almost instantly. It doesn't matter how pure of heart they are, how noble their quest is, or that they love their family very much: They're not trained, they're not qualified, and they're doomed from the moment they set out.
While there's far, far too many instances of people failing at trying to do something they can't in real life, having someone fail immediately at the big, important task/quest they set out to achieve seconds after starting can make for some great comedy, as poor Frodo demonstrates. It gets even funnier if they're The Chosen One who have to save the world, which can then lead to all sorts of interesting situations: What do the other characters do now that the Chosen One has failed/is dead? Do they try to fulfill the quest? Realize that it was a terrible idea to send a child out to do an adult's job? And if they do try to pick up the quest, do they have any chance at all at succeeding, since they weren't chosen by fate/prophecy/God?
Failure can be funny, but it can also open up new avenues and scenarios in storytelling that are otherwise underutilized.
Armageddon. Ragnarok. The end of all things. Almost every culture and mythology has its version of the moment when everything ends and the human race is wiped out.
But what if someone survived?
A few days ago, I came across the above music video, which tells the story of a Chinese astronaut who devotes his life to making it to the Moon, only for it to be all for nothing. Though only a little over three minutes long, it's a gripping story, so let's take a look and see what it does well.
Though he gets no lines, or even a name, the astronaut in the video gets our attention thanks to his strength of will: Thanks to a very efficient montage, we see him devoting everything to accomplishing his dream of getting on the Moon, sacrificing joy, happiness, or even love. Yet, we still root for him to succeed: How many of us wish we could devote every waking moment of our lives to accomplishing our dreams? Sadly, many of us can't, which makes us envious and (begrudgingly) admiring of those who can. The same goes for fictional characters. Perhaps your character wants to become president, or go into outer space, or win a spelling bee. Seeing them striving to accomplish a dream makes for compelling drama as they learn whether their dream is really worth everything they're sacrificing.
What would you do if the world was destroyed, and you were the only survivor? What would you do? How would you live? Would you even try to, or let yourself succumb to despair and throw yourself out the airlock? In the case of the music video, the astronaut realizes that what really mattes to him most is achieving his second chance of experiencing love, and he stops at nothing to make it happen.
While destroying the world shouldn't be done lightly in any medium, doing so has the advantage of forcing a character to confront reality without all the masks that they put up to protect themselves from other people and society at large. Do they go nuts and indulge their every whim? Succumb to despair and meaninglessness? Or do they defy the odds and refuse to give in, searching for others who might have survived, or chronicling everything for whatever sapient life form comes after them? There are countless possible answers, and wanting to see what happens to isolated, vulnerable characters will keep an audience engaged in our stories.
When something big – like a building, a city, or a planet – is destroyed, it creates a lot of debris and wreckage. In some situations, it gives us an opportunity for a unique action scene of characters having to escape said debris. 'Let's Go' has the astronaut fighting to reach the space station before it gets out of reach, but to do so, he has to go through a debris field consisting of wreckage from Earth, making it extremely difficult for him to reach his goal, and with the promise of certain death if he fails.
When writing our own stories, having to navigate the ruins of a wrecked mega-structure can lead to some exciting action scenes that we don't get to write very often. If the opportunity arises, embrace them and milk them for all they're worth. One of my favorite examples comes from 2016's 'Independence Day: Resurgence,' in which David Levinson and friends have to dodge falling debris sucked up from all over the Earth that subsequently rains down on London.
In disaster movies, it's very common for the survivors and main characters to either find a way to stop the disaster, or start rebuilding afterwords, hopeful and upbeat that one day, things can return to normal.
But what if they couldn't? What if they had no chance at all of rebuilding, or surviving, and death is inevitable?
One type of story we don't see too much of these days is the disaster story where there's no way for the characters to survive in the long run. It's easy to understand why: Audiences want a happy ending, or a hopeful one. A story where everyone is going to die, won't leave them feeling good after leaving the theater, closing the book, or turning off the game console. But if we choose this path, writers have a unique opportunity to explore what characters might do if they only have a little time left to live. Will they weep? Try to make peace with their god? Resolve any lingering conflicts with their loved ones? Or will they accept it and try to have fun before the end? Both astronauts choose the last option in 'Let's Go,' deciding to play video games together as they drift off into the void.
Choosing the path of inevitable death need not be dark. It can be sad, but it also gives characters one more chance to enjoy themselves, or to choose how they will spend what little time they have left. And if they go out having fun, or healing long-simmering hurts, that can be just as uplifting as a happy ending.
Our audience will always admire a protagonist or antagonist who has a goal and obsessively pursues it, even at a cost of personal happiness. They'll be even more interested if that individual is the only survivor of a world-ending event, and applies that determination to surviving or continuing on, no matter how bad things get. But if they are doomed, those characters can become their most interesting selves when they have to decide how to spend what little time they have left.
Favorite Moments: It's a Giraffe!
It takes one of the greatest film moments of the 1990's and gives Alan Grant the voice of Patrick Starfish. That's all you need to know.
Okay, maybe a more detailed explanation is in order. I adore how taking the audio from an episode of Spongebob Squarepants changes the mood and feel of such an emotional scene by making it comedic. It's probably a cosmic law that adding circus music to anything makes it funnier, no matter how dramatic, heartwarming, or awe-inspiring it is, a law I'd love to explore and see if it's true or not. But until then, I'll continue chuckling at Dr. Grant acting like an over-excited starfish.


My paper cut costume renderings draw inspiration from Swedish folk art. The colours and simplified symmetrical shapes embody the spirit of the floral embroidery found on traditional folk costumes. My imaginary production of The Birds is staged in a provincial theatre in Dalarna, where I grew up, and traditional art forms remain influential.
The themes in our play are timeless and universal and well suited to be interpreted by any culture anywhere. Most of us wish that we could escape from the pressures of life, but no matter how far away we go, we find that we are still ourselves. Perhaps it is not a place we seek at all. Perhaps it is in the pursuit of our craziest dreams that we find our most successful transformations- our Clodcuckooland.


Heading into this Friday's RFA 8 show, there are no shortage of top prospects, among them Lance "The Party" Palmer.
Since making his debut in 2011, Palmer has gone undefeated in his first six fights, including his most recent bout where he defeated nearly 40-fight veteran Patrick Reeves at Showdown Fights 11.
"It was more of a filler fight because I hadn't fought since November, and it felt good to get back in there," said Palmer. "It was a good fight for me, the guy had a lot of fight, but I went out and got the finish in the first round with a guillotine from mount.
While he's done well in his career so far, Palmer feels the recent addition of a UFC vet to his coaching staff has helped him raise his game to the next level.
"It all starts with Duane Ludwig," said Palmer. "He's been helping mold me and get rid of my bad habits and pick up some good ones.
It's those additional skills that will come in handy when Palmer faces his most difficult task to date in fellow top up-and-comer Jared Downing (9-1) in what could be a sleeper candidate for Fight of the Year.
"On the ground, against the cage, anywhere this fight goes, I'll be comfortable," Palmer told MMaWeekly.com. "He's a grinder and is going to want to take it there, so that's perfect for me.
Palmer, along with Downing and RFA 8 headliner Sergio Pettis are considered by many to be fighters who will be taking the next step forward in their careers in the coming months.
And even though he's thought of so highly, Palmer feels nothing is a given, and in order to take that next step, he has to have a standout performance Friday night.
"I need a great performance this week and then I'll take a little break and then see what the next step is from here," he said. "It all depends on me going out there and getting a victory first.


text:
246 ROBERT ST, Baltimore, MD, 21217
Courtesy of: John Rob Real Estate Group
Address: 246 ROBERT ST
County: Baltimore City
City: Baltimore
Neighborhood: Spicer's Run At Bolton Hill
It's All About Location, Location, Location!!! And, that's what you'll find in the desirable and spectacular Bolton Hill location. What a "GREAT OPPORTUNITY" to own this beautiful three-bedroom, two and a half bath brick townhouse in the private community of Spicer's Run, it's the heart of the historic community of (Bolton Hill). In addition, it has a 2 car garage with driveway parking, spacious rooms, large eat-in kitchen with tons of countertop space and so much more. Very convenient to I-83, The Lyric Opera House, MICA, Light rail, Penn Station, Amtrak train & downtown Baltimore is just a 3 minute drive. THIS HOME IS A MUST SEE!
Courtesy of: John Rob Real Estate Group Listing information provided courtesy of the Bright MLS. IDX information is provided exclusively for consumers' personal, non-commercial use, and it may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing. The data is deemed reliable, but is not guaranteed accurate by the MLS. Updated: 1st February, 2023 11:52 AM (UTC)