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O2TvSeries: Stream And Download Latest TV Shows On Your Phone
In this post, we are going to examine how to easily stream the latest TV shows online for free or download Popular TV shows to your phone without paying a single cent using o2Tvseries.
There are many rules that govern how TV shows are being watched in different parts of the world.
In the UK, US for example where most of these content creators originate from you're meant to be having about 2 to 4 movie, TV show downloading subscription services like Netflix, a HBO account if you want to watch the latest game of thrones episodes as soon as they go live.
That's not all, there's Hulu, Amazon prime and many others, even more in works, that's if you've heard of the upcoming Disney+.
These services are nice, however, they cost money and most importantly are not available in most countries.
For example to get yourself a HBO account in Nigeria, you'd have to do it illegally, by tweaking your IP address so as to be able to subscribe.
That and many other issues like most interesting movies and TV shows on Netflix that is even available globally are restricted in different regions. For example, I can't watch, Avengers: Infinity war on my Netflix account except I'm from the UK or the US and we both pay the same amount for this subscription? Sighs.
So How Do I keep up with the Latest Shows?
The unavailability of these shows in your region shouldn't, doesn't mean you can't watch them or at least keep up.
In fact, with websites like Goojara, and the O2tvseries we're talking about today, you can stream or download most of your favourite and latest TV shows without paying a dime.
Just one catch, you'll be bombarded with ads.
It's not big deal though provided that you're not paying for the content you're viewing and that's how the site runners get to fund the site to keep it free, you just need to find your way through to the content you're looking for.
How to download and watch TV shows online with O2tvseries free?
That's what we're going to be examining in this section.The shows uploaded on O2tvseries have been dubbed down for mobile phone quality which means no big file sizes or losing unnecessary data for streaming.
To get started, determine if you enough data to stream or download or which one you want to be doing in essence.
Once that is done, jump on a browser like UC browser, its the one one recommend for operations like this, here's why.
Go to the site homepage, www.O2tvseries.com and you'll see the latest updates of the latest TV shows like below. But that's not where you download. That's just a list of latest additions that are updated everyday BTW. You'll need to scroll down to the list of shows that are categorised by the beginning of the show title letters and grouped into three letters each, for example, shows with name starting from A, B, C – D, E, F and so on.
It looks like below, so if you're looking to stream or download a show like Game of Thrones on O2tvseries, you'll need to click on the G, H, I section. After that then you'll see a page with a list of shows starting from those three letters, depending on how recently they've been updated.
If not updated recently then you'll need to use the next button to locate your show but for the for this tutorial, we'll be downloading the latest episode of game of thrones.
Clicking on the show name should take you to a page like below, note: Due to the pop ads placed by the site owners the first clicks usually takes you to a weird ad, just close the tab and return to your page.
As you can see, all the seasons are listed. This isn't like that for all the shows but for a popular one like this? Everything should still be intact. Just click on the season and a list of episodes and ads should follow as well, as usual ignore them and select your episode to land on a page like below.A list of the episode you want in three formats.
HD MP4
3GP (Low format, do go this way unless you really really have less data and hate the show)
Usually the first option is okay but the second is better.Click on it and solve the captcha, little test to determine if you're human and you'll get two options.
Watch online (Stream)
Download to your device.
Anyone you go for is absolutely fine. What matters is that you enjoy the show. I usually do a mix of both… Watch online when I want a quick fix or save for later when I'm less busy and have nothing to do.
That's it, you've successfully learned how to download shows on O2tvseries. You can apply this little tutorial when downloading other shows. It's the same way.
Are There Any O2tvseries alternatives?
I felt this section should be added because well sometimes these guys mess-up with the shows or don't have it available on their website at all.
The ads are also a bit much but it's the price we pay for free stuff, when you're feeling not up to it, here are some O2tvseries alternatives you can try.
Goojara.ch (Read our full download guide)
TVshows4mobile.net (Just exactly like O2tvseries)
Toxicwap.com
Todaytvseries2.com (HD 480p quality free shows download)
300mbfilms.com (Way too much ads but shows in high quality version)
With these guys in place and your ISP with the data or WiFi? You can watch most TV shows online for free.
If you're facing any difficulties carrying out any of these steps, leave a comment below, Let's trash it out together yeah?
Thanks for stopping by. Join 1000 of us on Facebook Here for free updates.Read How to Get a Netflix Subscription In Nigeria, For Cheap Next.
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Notch signalling regulates cell fate in most tissues, promoting precursor cell proliferation in some, but differentiation in others. Accordingly, downregulation or overactivity variously contributes to cancer development. So far, little is known about Notch pathway activity and function in the normal urothelium and in urothelial carcinoma (UC). We have therefore investigated expression of Notch pathway components in UC tissues and cell lines and studied the function of one receptor, NOTCH1, in detail.
Expression of canonical Notch pathway components were studied in UC and normal bladder tissues by immunohistochemistry and quantitative RT-PCR and in UC cell lines and normal cultured urothelial cells by qRT-PCR, immunocytochemistry and Western blotting. Pathway activity was measured by reporter gene assays. Its influence on cell proliferation was investigated by γ-secretase inhibition. Effects of NOTCH1 restoration were followed by measuring cell cycle distribution, proliferation, clonogenicity and nuclear morphology.
NOTCH1 and its ligand, DLL1, were expressed at plasma membranes and in the cytoplasm of cells in the upper normal urothelium layer, but became downregulated in UC tissues, especially in high-stage tumours. In addition, the proteins were often delocalized intracellularly. According differences were observed in UC cell lines compared to normal urothelial cells. Canonical Notch pathway activity in reporter assays was repressed in UC cell lines compared to normal cells and a mammary carcinoma cell line, but was induced by transfected NOTCH1. Inhibitors of Notch signalling acting at the γ-secretase step did not affect UC cell proliferation at concentrations efficacious against a cell line with known Notch activity. Surprisingly, overexpression of NOTCH1 into UC cell lines did not significantly affect short-term cell proliferation, but induced nuclear abnormalities and diminished clonogenicity.
Our data indicate that canonical Notch signalling is suppressed in urothelial carcinoma mainly through downregulation of NOTCH1. These findings can be explained by proposing that canonical Notch signalling may promote differentiation in the urothelium, like in many squamous epithelia, and its suppression may therefore be advantageous for tumour progression. As an important corollary, inhibition of canonical Notch signalling is unlikely to be efficacious and might be counter-productive in the treatment of urothelial carcinoma.
Bladder cancer is the fifth most common malignancy in most Western industrialized countries with urothelial carcinoma (UC) representing the major histological subtype. UC can be further classified into papillary and invasive carcinomas . While most cases of papillary low grade urothelial tumours have a good prognosis, high grade papillary tumours tend to progress towards invasive cancers which are often lethal. A large fraction of invasive urothelial carcinomas develop by a different route via high-grade dysplastic carcinoma in situ . Depending on tumour stage, urothelial carcinoma treatment is treated either by transurethral resection or radical cystectomy. In addition neo-adjuvant or palliative chemotherapy is used in locally advanced or metastatic urothelial carcinomas, respectively [3, 4]. Local immunotherapy or chemotherapy is used for preventing recurrences of superficial cancers. Until now, clinical studies using molecular targeted drugs have yielded disappointing results [5–7]. This failure is generally considered to reflect our insufficient knowledge of urothelial tumour biology.
The Notch signalling pathway is a promising target for cancer therapy [8, 9]. In many tissues, Notch signalling contributes to the maintenance of stem cells or precursor cells  and pathway hyperactivation caused by point mutations  or translocations involving NOTCH genes [12, 13] is oncogenic by blocking cell differentiation, protecting against apoptosis and stimulating proliferation. Conversely, in certain epithelia, Notch signalling promotes differentiation. Accordingly, pathway inactivation, frequently caused by NOTCH1 mutations, is observed in squamous carcinomas of several organs [14–16]. Therefore, depending on tissue and tumour type, drugs targeting Notch signalling may be therapeutic, useless or tumour-promoting .
Notch signalling constitutes a short-range communication system between adjacent cells. Canonical Notch signalling is activated by ligand-receptor interactions releasing the receptor intracellular domain (NICD) by two proteolytic steps, including an essential cleavage by γ-secretase. The NICD translocates into the nucleus where it binds to C Promoter Binding Factor-1 (CBF-1), recruits co-activators (MAML1 and KDM5A) and typically activates the transcription of Hairy and Enhancer of Split (HES) and Hairy and Enhancer of Split related (HEY) target genes. Through such effectors Notch determines cell fate .
To date, little information exists on the Notch signalling pathway in normal urothelium or urothelial cancers. Decreased expression of several Notch receptors and ligands in immunohistochemistry has been reported especially in papillary UC . We have investigated the expression of Notch pathway components in urothelial carcinoma tissues and cell lines and the function of NOTCH1 in UC cell lines.
The benign and tumour bladder samples used for gene expression studies were a subset of those described previously  comprising 11 benign bladder tissues and 30 bladder cancer tissues from 25 male and 5 female patients with ages ranging from 54 to 84 years. Tumour stages and grading according to the UICC classification were as follows: pT3 G3 in 11 cases, pT4 G3 in 6 cases, pT2 G2 in 6 cases, pT2 G3 in 3 cases and one case each of pT3 G2, pT1 G2, pTa G3 and pTa G2 tumours. The set used for immunohistochemistry comprised 4 normal tissues, 27 UC tissues with 6 pTa low grade, 5 pTa high grade, 5 pT1, 3 pT2, 3 pT3, 4 pT4, 1 CIS. The study was approved by the ethics committee of the medical faculty of the Heinrich Heine University and informed consent was obtained from all patients.
All UC cell lines (5637, 639v, 647v, BFTC905, HT1376, J82, RT4, RT112, SD, SW1710, UM-UC3, VM-Cub1, T24) and HEK293 cells were cultured in DMEM GlutaMax (Gibco, Darmstadt, Germany), supplemented with 10% fetal calf serum . They were obtained from the DSMZ (Braunschweig, Germany), except for UM-UC3, kindly provided by Dr. Grossman, Houston. The well-differentiated UC cell line BC61 was cultured and characterized as previously described [22, 23]. Primary urothelial cells (UP) were prepared and maintained as described (20). T47D cells were maintained in RPMI1640 supplemented with 15% foetal calf serum and 10 μg/ml insulin.
Human N1ICD-pIRES2 was a kind gift from Prof. Giebel, Essen; human full-length wildtype NOTCH1 in a pcDNA3-HA/FLAG vector was kindly provided by Prof. Di Fiore, IFOM, Italy .
Cells were treated with 0.25 - 20 μM γ-secretase inhibitors DAPT (#2634, TOCRIS/R&D Systems), L-685,458 (#2627, TOCRIS/R&D Systems) or Compound E (#56597, Calbiochem/Millipore) or, as a control, compound W (#2654, TOCRIS/R&D Systems) for 48 h when viability was analysed by MTT assay.
For reporter gene assays, cells seeded in 6-well plates were transiently transfected with XtremeGene9 (Roche) with 1 μg of either pJH26A (Notch responsive reporter) or pJH28A (Notch non-responsive reporter), kindly provided by Prof. D. Hayward , or in other experiments either pHES1 (+CBF1)-luc (addgene 41723; similar to pJH26A) or pHES1 lacking the CBF1 site (addgene 43805, similar to pJH28A). p850-luc and pGL3 (Promega) were used as positive or negative controls, respectively. NOTCH1 expression plasmids were added as indicated in the individual experiments, using the respective plasmid vectors for adjusting total amounts transfected. All cells were co-transfected with 10 ng Renilla luciferase plasmid for normalization. Cells were harvested 48 h after transfection and assayed for luciferase activities by the Dual-Luciferase reporter assay (Promega). Notch activity was determined as the ratio of wildtype to mutant plasmids and normalized to p850-luc and Renilla luciferase activity.
The ligand-dependent activation assay was carried out as described . Briefly, urothelial cancer cell lines seeded in 6 well plates were co-transfected at 80% confluency with NOTCH1 full-length expression plasmid (or vector), the Notch-responsive reporters pJH26A or pJH28A and Renilla luciferase plasmid at a 1:1:0.1 ratio. After 24 h cells were replated in triplicates in 24 well plates coated with ligand or controls. For that purpose, the wells were first coated with 20 μg/ml goat anti-human IgG1C Fc secondary antibody (ThermoFisher Scientific, Germany), washed, and then incubated for 2 h with 10 μg/ml DLL1 extracellular domain (aa 1–545) fused at its C-terminus to the Fc portion of human IgG (DLL1:Fc; AdipoGen, Switzerland) or human IgG (ThermoFisher Scientific, Germany), followed by a further wash. After a further 24 h, cells were harvested and assayed for luciferase activities by the Dual-Luciferase reporter assay (Promega). Basal activity in cells exposed to immobilized IgG was set as 1 for each cell line.
For the colony forming assay 105 cells were seeded in 6 well plates and transfected with pIRES2-N1ICD or the corresponding pIRES2 empty vector, or with pcDNA4/to-Notch1 full-length (N1-FL) or the corresponding pcDNA4/to-lacZ plasmid. After 24 h cells were seeded in triplicates into 10 cm plates and selection for stable integration of the plasmids was started the next day. Cells transfected with pIRES2 plasmids were treated with 1.0 - 1.8 mg/ml Geneticin Selective Antibiotic G418-Sulfat (Invitrogen) and cells transfected with pcDNA4/to-plasmids were treated with 100–150 μg/ml Zeocin Selection Reagent (Invitrogen) every two days for 2 weeks. After a one week recovery period with lower antibiotic concentrations, the remaining colonies were fixed with 100% methanol and stained with Giemsa solution (Sigma-Aldrich, Germany). Colonies were then counted and statistical comparisions were made by Student's T-Test.
Total RNA was isolated from sub-confluent cell lines using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA was reverse transcribed using 200 U SuperScriptII reverse transcriptase (Invitrogen, Darmstadt, Germany), with 300 ng oligo-dT and 25 ng random hexamer primers. Real Time-PCR assays were performed with the ABI7900HT System using the QuantiTect SYBR Green PCR Kit (Qiagen) and premade or self-designed primers (Additional file 1: Tables S1–S2). TBP was used as a reference gene. Statistical comparisons were made by Mann–Whitney U test with SPSS 21.
Cells grown to 70% confluence were fixed with 100% ice-cold methanol for 5 min, permeabilized in 0.1% Saponin-TBS solution and blocked in 1% bovine serum albumin in 0.2% Tween-TBS for 30 min each. Cells were then incubated with the primary antibodies NOTCH1 (Epitomics, EP1238Y), JAG1 (Santa Cruz Biotechnology, H114), DLL1 (Santa Cruz Biotechnology, H-265; Abcam, ab84620) and NOTCH2 (Abcam, ab8926) and subsequently with goat-anti-mouse Alexa Fluor488 antibody. Nuclei were stained with 1 μg/ml DAPI (Dako, Hamburg, Germany).
Subconfluently grown cells were lysed in RIPA buffer with protease and phosphatase inhibitors. Western blot analysis after SDS-page was performed using primary antibodies against NOTCH1 (Epitomics, EP1238Y), JAG1 (Santa Cruz Biotechnology, H114), DLL1 (Santa Cruz Biotechnology, H-265; Abcam, ab84620), NOTCH2 (Abcam, ab8926) and α-tubulin (B-1-5-2, Sigma) as a control. Secondary antibodies labelled with HRP and the ECL advanced or ECL Select kits (GE Healthcare) were used for detection.
Immunohistochemistry was performed by standard techniques with antigen retrieval by citrate buffer pH 9. Antibodies used were NOTCH1 (Epitomics), JAG1 (Santa Cruz) and DLL1 (Santa Cruz). Detection was achieved via a biotin-conjugated polyvalent antibody with an avidin-biotin-peroxidase reagent (Scy Tek, Logan, USA) and diamino-benzidine staining. Sections were counter-stained with hemalum. The staining results of the reference tissues for antibody specificity are provided in Additional file 1: Figure S1.
Expression of several Notch pathway components was measured at the mRNA level by qRT-PCR in a well-characterized set of invasive urothelial carcinoma and normal bladder tissue samples that we had previously used for other studies . Expression of NOTCH1 and NOTCH2 was significantly decreased in almost all urothelial cancers (Figure 1A-B). NOTCH3 expression was generally unchanged in cancer tissues. NOTCH4, which marks endothelial cells, was strongly expressed in bladder tissues but rarely detectable in cultured cells (data not shown). Among the ligands, DELTA1 (DLL1) expression was strongly and significantly decreased in cancer tissues, whereas JAGGED1 (JAG1) and JAGGED2 (JAG2) mRNA levels were not significantly changed (Figure 1C-E). Expression of DELTA3 and DELTA4 was undetectable in normal and cancer tissues. None of the nuclear components, KDM5A, MAML1, SKIP and CBF1 showed significant expression changes between normal and cancer tissues (Figure 1F-I). Expression of HES1 and HEY1 was rather enhanced in cancer tissues, reaching significance for HES1, whereas HES5 was unchanged (Figure 1J-L).
Expression of Notch pathway components in bladder tissues. Relative mRNA expression of Notch pathway components in 11 benign and 30 tumour bladder tissue samples was measured by qPCR for the Notch receptors (A) NOTCH1, (B) NOTCH2, for the canonical ligands (C) DLL1, (D) JAG1, (E) JAG2, nuclear factors (F) CBF1, (G) SKIP, (H) MAML1, (I) KDM5A as well as the potential Notch target genes (J) HES1, (K) HES5 and (L) HEY1. Data are represented as box plots. Statistical comparisons between benign and tumour bladder tissue expression were made by the Mann–Whitney U Test using SPSS version 21.
Expression of NOTCH1, DLL1 and JAG1 was further analysed by immunohistochemistry in a set of paraffin-embedded tissues covering urothelial carcinomas of all stages and grades (Table 1). In normal urothelium, NOTCH1 was predominantly localized at the membranes of intermediate cells and, more strongly, of umbrella cells. Staining of basal cells was weak. No nuclear staining was observed in UC compared to breast cancer (Additional file 1: Figure S1A). The distribution of DLL1 was very similar. JAG1 was observed in the cytoplasm and at the membranes of lower urothelial layer cells (Figure 2). Expression and intracellular distribution of the three proteins changed in urothelial carcinomas (Figure 3A). NOTCH1 and JAG1 decreased with increasing tumour stage and became undetectable in several high stage tumours. In low grade papillary tumours both proteins remained mainly associated with the plasma membrane, whereas residual protein in higher grade and stage cancers was essentially cytoplasmic. DLL1 remained expressed in low grade papillary tumours, albeit with a more cytoplasmic localization. In higher grade and stage cancers, DLL1 was diminished, often heterogeneously expressed and delocalized to the cytoplasm and focally even to nuclei (Figure 3B). An overview of the staining results is provided in Table 1.
Intensity of staining was rated by two independent observers as 3 (strong compared to normal bladder urothelium), 2 (comparable to normal), 1 (weak or very weak compared to normal), 0 (undetectable).
Immunohistochemical detection of NOTCH1 and its ligands DLL1 and JAG1 in a benign bladder tissue. In this representative benign urothelium (corresponding normal tissue from case #15) NOTCH1 and DLL1 are localized in the cytoplasm and plasma membranes of umbrella cells and the membranes predominantly of intermediate cells. Staining in the basal layer is weak, especially for NOTCH1. JAG1 is conversely detectable more strongly in the lower cell layers, mostly in the cytoplasm and weakly at the plasma membrane.
Immunohistochemical staining of NOTCH1 and its ligands DLL1 and JAG1 in representative bladder tumour tissues. (A) NOTCH1, DLL1 and JAG1 staining decreased with tumour invasiveness and became undetectable for NOTCH1 and JAG1 in several muscle invasive bladder tumours. (B) Two examples for intratumoral heterogeneity of DLL1.  membrane staining,  nuclear staining,  no detectable staining.
In UC cell lines NOTCH1 mRNA was likewise decreased compared to normal cultured urothelial cells (UP), whereas NOTCH2 expression was only slightly diminished. Expression of DLL1 was strongly reduced, but that of JAG1 and JAG2 less so (Figure 4). No significant differences were observed for the nuclear components CBF1 and SKIP whereas KDM5A and MAML1 were strongly expressed in several UC cell lines. Expression of the putative target genes HES1 and HEY1 was increased in individual UC cell lines only.
Expression of Notch pathway components in urothelial cells. Relative mRNA expression in 8 normal urothelial cell cultures (x) and 16 urothelial cancer cell lines, subdivided in 7 papillary UC (♦) 9 invasive UC (■) as well as 3 non-UC bladder cancer cell lines (▲), was measured by qPCR for the Notch receptors (A) NOTCH1, (B) NOTCH2, for the canonical ligands (C) DLL1, (D) JAG1, (E) JAG2, the nuclear factors (F) CBF1, (G) SKIP, (H) MAML1, (I) KDM5A as well as the potential Notch target genes (J) HES1, (K) HES5 and (L) HEY1.
As in tissues, the changes in the mRNA expression of Notch pathway components in cell lines extended to the protein level (Figure 5). Normal urothelial cells (UP) expressed robust amounts of the processed cytoplasmic NOTCH1 fragment, which was depleted severely in all UC cell lines. DLL1 was likewise more strongly expressed in UP and in cell lines from papillary tumours. Contrasting with the general downregulation of NOTCH1 and DLL1, JAG1 protein remained comparable between UC and normal cells. NOTCH2 signals likely corresponding to full-length NOTCH2 and N2CTD were rather increased in cell lines with a mesenchymal phenotype (Figure 5). Immunocytochemistry revealed membranous and cytoplasmic localization of NOTCH1 and JAG1 in papillary UC lines and weak cytoplasmic staining in invasive UC cells (Additional file 1: Figure S2). As in tissues, DLL1 was often heterogeneously distributed between cells with nuclear or cytoplasmic staining. NOTCH2 was predominantly localized in nuclei in papillary urothelial cell lines, but predominantly cytoplasmic in invasive urothelial cell lines.
Expression of Notch pathway component proteins in urothelial cells. Western Blot analysis of Notch ligands and receptors in urothelial cancer cell lines compared to a primary normal urothelial cell culture (UP). Top to bottom: Analysis with a JAG1 antibody resulted in two signals at approximately  = 170 kD and  = 130 kD, likely corresponding to JAG1 full-length protein and its processed extracellular domain. Signal , in particular, was strongly decreased in invasive UC cell lines compared to normal urothelial and papillary UC cells. DLL1 protein was detectable at the expected size of 75 kD . The signal was decreased in invasive UC cell lines compared to papillary UC and normal urothelial cells. Analysis with NOTCH1 antibody resulted in an approximately 120 kD band  indicating a C-terminal NOTCH1 fragment which was strongly diminished in urothelial cancer cell lines. Band  at ~300 kD likely corresponds to full-length NOTCH2 protein and band  at ~120 kD to the processed C-terminal NOTCH2 fragment. No general decrease in expression is discernible in the UC cell lines. α-Tubulin (Sigma) was used as a quality and loading control.
To measure canonical Notch signalling activity at its final step in the nucleus, we applied a pair of reporter gene plasmids with multiple binding sites for CBF1 functional in pJH26A, but mutated in pJH28A. Their activity ratio reflects the nuclear output of the pathway. As a positive control, the mammary carcinoma cell line T47D yielded a pJH26A/28A ratio of 11:1 indicating the expected high pathway activity (Figure 6A). UC cell lines showed ratios between 1:2 and 1:5 (Figure 6B) indicating a repressed state. The ratios in normal UP varied between individual cultures from weak repression (1:2) to moderate activation (3:1). Co-transfection of a plasmid expressing active N1ICD further increased Notch nuclear signalling in normal UPs (Figure 6C) and also in the UC cell lines showing that the Notch signalling pathway can in principle be activated by overexpression of NOTCH1. However, Notch reporter activation increased with higher amounts of plasmid in UPs, but not in carcinoma cells (Figure 6D), suggesting a toxic effect or strong negative feedback factors in the latter. More moderate activation was achieved by transfecting a plasmid expressing unprocessed NOTCH1 (N1-FL). Expression of both N1ICD and N1-FL after transfection was verified by western blotting (Figure 6E-F).
Notch signalling pathway activity in urothelial cells. Notch activity was determined by the ratio of the Notch-dependent wildtype plasmid pJH26A to the Notch-independent mutant plasmid pJH28A. p850-luc and pGL3 plasmids were used as controls. (A) The mammary carcinoma cell line T47D showed a ratio 11:1 suggesting Notch activity. (B) Results of reporter gene assays in primary urothelial cell cultures (UP) and urothelial cancer cell lines in comparison to T47D. (C-D) Induction of Notch activity after transfection of pIRES2-N1ICD in (C) two normal urothelial cell cultures (UP234 and UP188) or (D) UC cell lines BFTC905, VM-Cub1 and 5637. In contrast to the UP cultures, in UC lines Notch activity decreased upon further increasing plasmid concentration (light grey BFTC905, dark grey VM-Cub1, black 5637). (E) Successful overexpression of N1ICD and NOTCH1 full-length (N1-FL) plasmids after 24 hours in UC lines proven by Western Blot with a Notch antibody detecting the C-terminal fragment (Epitomics) with α-Tubulin as a loading control (F) inducing Notch activity in reporter assays. pJH26A to pJH28A ratios are normalized to the empty vector pIRES2. (G) Reporter gene assay with a HES1 promoter with intact or without CBF1 binding sites driving luciferase expression in the four UC lines. Basal luciferase activities were not significantly different between the reporters, whereas co-transfection of the N1ICD fragment significantly activated the HES1 promoter. (H) Assay for DLL1-dependent activation of the Notch signalling pathway in VM-Cub1 and BFTC905 cells. Notch pathway activity was measured by reporter plasmids cotransfected with or without full-length NOTCH1 (N1-FL) and exposed to immobilized extracellular DLL1 domain or IgG. Changes in the pJH26A/pJH28A reporter ratio were not significant between IgG vs. DLL1 exposure (independent of N1-FL), whereas induction by N1-FL was highly significant (T-tests p < 0.01) in each case.
To further investigate Notch signalling at the transcriptional level, we additionally applied another pair of reporters containing the natural HES1 promoter with intact or mutant CBF1 binding sites. As shown in Figure 6G, both HES1 reporters yielded comparable luciferase activity under basal conditions in four different UC cell lines, indicating inactive nuclear Notch signalling. Co-transfection of the active N1ICD domain activated the wildtype promoter in all four cell lines.
To obtain an indication whether lack of ligand expression contributed to Notch pathway inactivity, we investigated the effect of extraneous DLL1 ligand, as this ligand was most prominently reduced at mRNA and protein levels in UC tissues and cell lines. For that purpose, we employed an experimental setup, in which the extracellular domain of the DLL1 ligand is immobilized as a Fc-fusion protein to cell culture plates through anti-Fc-antibodies . Two urothelial cancer cell lines, BFTC905 and VM-Cub1, transfected with full-length NOTCH1 or vector were seeded on the coated plates and Notch pathway activity was determined via co-transfected Notch reporter plasmids pJH26A and pHJ28A. As in the previous experiments, Notch signalling was induced by transfection of full-length NOTCH1, but neither further enhancement nor increased basal activity were achieved by exposure to the immobilized extracellular DLL1 domain (Figure 6H). This finding suggests that NOTCH1 is the most limiting factor for canonical Notch activity in UC cell lines.
If canonical Notch signalling is indeed inactive in UC, inhibition of the essential γ-secretase cleavage should not affect cell proliferation. Therefore, we analysed the effects on cell proliferation and cell survival after pharmacological γ-secretase inhibition in urothelial cells. Three inhibitors, i.e. DAPT, L-685,458 and Compound E, inhibit the protease presenilin forming part of γ-secretase in general, whereas Compound W specifically blocks the processing of one substrate (APP), providing a specificity control. Indeed, in HEK293 cells which are sensitive to inhibition of Notch signalling, the former three inhibitors, but not Compound W, inhibited proliferation in a dose-dependent manner (Figure 7). In contrast, the specific γ-secretase inhibitors did not inhibit proliferation of the UC cell lines better than Compound W. Rather, DAPT and L-685,458 tended to increase vital cell numbers.
Effects of treatment with γ-secretase inhibitors (GSI) on UC proliferation. The indicated UC cell lines and HEK293 cells were treated with the indicated concentrations of DAPT, L-685,458, Compound E and the modulator Compound W for 48 hours after which cell viability was analysed by a MTT-Assay.
Whereas pharmacological Notch signalling inhibition did not diminish proliferation in urothelial cancer cell lines, overexpression of N1-FL or N1ICD diminished clonogenicity of urothelial carcinoma cell lines (Figure 8A). In short term transfections, neither protein induced substantial apoptosis or cell cycle arrest. Accordingly, the number of transfected cells in four cell lines declined only weakly over three days by at most 45% (data not shown). However, between 35% and 64% of N1ICD-transfected cells displayed various striking nuclear abnormalities compared to controls, especially a tendency towards multinuclearity and instances of mitotic catastrophes and nuclear fragmentation (Figure 8B).
Effects of NOTCH1 re-expression on UC cell lines. (A) Five independent colony forming assays with triplicate plates for each experiment after transfection of plasmid constructs expressing NOTCH1 intracellular domain (N1ICD) or full-length protein (N1-FL) compared to control constructs. Clone numbers were counted after selection for three weeks with either G418 (pIRES-N1ICD/pIRES-2) or Zeozin (pcDNA4to-N1-FL/pcDNAto-lacZ). The student's T Test was carried out for statistical analysis. Colony forming potential was significantly decreased in N1ICD and N1-FL transfected cells. (B) Nuclear abnormalities typically appearing after transfection of N1ICD into UC cell lines. Note nuclear enlargements, deformation, micronuclei, fragmentation, multinuclearity and mitotic catastrophe in cells expressing N1ICD (green) as compared to others. DNA was counterstained with DAPI (blue). Further analysis is shown in Additional file 1: Table S4.
Taken together, our results from tissues and cell lines suggest that canonical Notch signalling towards the nucleus is inactive in urothelial carcinoma, especially in the invasive subtype. NOTCH1 and DLL1 were most consistently diminished in both cell lines and tissues. Downregulation of these factors is in accord with a previous report , whereas another paper reported decreased NOTCH1 expression in high-grade invasive UC tissues only, with more pervasive cytoplasmic localization . The mechanisms underlying these changes remain to be determined. Sequencing data from the TCGA consortium (http://cancergenome.nih.gov/) indicate only few mutations in Notch pathway components in urothelial carcinoma (Additional file 1: Table S3). The frequency of NOTCH1 mutations is about 5% and is thus considerably lower than in head-and-neck squamous cell carcinomas . A more likely cause of downregulation are copy number changes at chromosome 9q34 (where NOTCH1 is encoded) which occur in up to 30% of invasive UC. However, neither mutations nor gene loss can fully account for the high prevalence of NOTCH1 downregulation. As both, NOTCH1 and DLL1, are predominantly localized in more differentiated cells in the normal urothelium, their decreased expression in tumours may conceivably reflect to some extent the loss of this differentiated cell population.
In addition to downregulation, we frequently observed delocalization of NOTCH1 and DLL1 to the cytoplasm in tumour cells. Delocalization of NOTCH proteins to the cytoplasm in some cells reflects lack of activation by external ligands in trans but enables cis-inhibition by ligands expressed in the same cell compartment [29, 30]. Candidates for that function are JAG1 and especially JAG2, which appear to be retained or in the case of JAG2 even upregulated in invasive UC . JAG1 was mostly cytoplasmic in tumour cells and is thus unlikely to contribute to trans-signalling. Notably, the distribution of JAG1 in normal urothelium is quite similar to that of p63 , a factor known to maintain basal cells in many epithelia and to induce JAG1 in many instances. The co-localization of DLL1 and NOTCH1 proteins in normal urothelium suggests that DLL1 could be the physiological ligand for NOTCH1 in this tissue. However, exposure of UC cell lines to immobilized DLL1, which is considered to yield superior activation compared to soluble ligand  did not enhance activation of a Notch-dependent reporter by transfected NOTCH1 protein, least activate canonical signalling by itself.
We note that NOTCH2 expression was less strongly changed than that of NOTCH1 and that active N2ICD could be detected in the nuclei of some UC cell lines. Interestingly, it appeared more strongly expressed in mesenchymal UC cell lines albeit localized to the cytoplasm. Increased expression of NOTCH2 in UC cell lines with a mesenchymal phenotype as compared to lines with an epithelial phenotype has recently been reported by others [33, 34]. The same authors observed that NOTCH2, but not NOTCH1, favors epithelial-mesenchymal transition and cell migration in UC cell lines [33, 34]. Collectively, these findings suggest divergent functions of NOTCH1 and NOTCH2 in UC, which have also been observed in other cancers [35, 36]. Nevertheless, despite the expression of N2CTD at a level comparable to that in normal urothelial cells, two different NOTCH-dependent reporters showed no evidence for canonical pathway activity in the UC cell lines. Likewise, γ-secretase inhibitors did not block cell proliferation in UC cell lines. These findings argue that canonical Notch signalling to the nucleus is inactivated in urothelial carcinoma. As a consequence, it seems plausible that the effects of NOTCH2 in mesenchymal UC cell lines, considering especially its cytoplasmic localisation, could be exerted by non-canonical signalling.
Across the cancer tissues, expression of HES1 mRNA was significantly increased compared to normal bladder, despite the significant downregulation of NOTCH1, NOTCH2 and DLL1. Although no upregulation of HES1 was observed in the UC cell lines compared to normal urothelial cells, this finding is somewhat puzzling, since HES1 is a common target gene of the canonical Notch pathway. However, target genes of the pathway vary strongly between tissues and even HES1 is not a universal target . Moreover, HES1 is regulated by several pathways, including Hedgehog , ATF2 , and signalling through JNK1 . In UC cell lines, a reporter plasmid driven by the HES1 promoter was similarly active, if the CBF sites were wild-type or mutant, supporting our conclusion that canonical Notch signalling is inactive. However, luciferase was induced by N1ICD cotransfection indicating that HES1 can be a target of an active Notch pathway in urothelial cells. Collectively, these findings suggest that the increased expression of HES1 in UC tissues could reflect its predominant regulation by a different pathway in the absence of canonical Notch signalling.
In normal urothelial cells and urothelial carcinoma cell lines, overexpression of full-length NOTCH1 or its active intracellular domain induced canonical reporters but was not compatible with long-term proliferation of UC cells. Inhibition of proliferation did not appear to occur by straightforward inhibition of cell cycling or by a major induction of apoptosis. Rather, NOTCH1 transfected cells often showed misshaped or abnormally large nuclei or became obviously binuclear. Intriguingly, in normal urothelium NOTCH1 is localized in the upper layers together with its likely ligand DLL1, where they might contribute to terminal differentiation. In particular, umbrella cells of the urothelium are usually polyploid . In squamous epithelia, Notch signalling is most important for establishing the commitment to terminal differentiation in the suprabasal layer [41, 42]. Notch signalling is well-established as a regulator of polyploidization in Drosophila, but may also act in this fashion in certain mammalian cells . Evidently, the urothelium is one tissue, where this function should be investigated in detail. The observed effects of NOTCH1 overexpression on UC cells may thus be conceptualized by assuming that it induces a failed attempt at differentiation. Evidently, the effects of pharmacological inhibition of the Notch pathway and genetic inactivation of individual components such as NOTCH1, DLL1 and NOTCH2 should be studied in culture and animal models of urothelial differentiation.
Collectively, our findings indicate that canonical Notch signalling is lost in urothelial carcinoma mainly via inactivation of NOTCH1, which in normal urothelium may promote specific steps of urothelial differentiation. As it has been shown that inhibition of the γ-secretase leads to hyperproliferation in tissues, where Notch signalling promotes differentiation , inhibition of Notch does therefore not appear to represent a useful therapeutic approach to UC. Instead, urothelial differentiation might become disturbed as an adverse effect of long-term treatment of other tumour entities with drugs targeting canonical NOTCH signalling.
We are grateful to Ms. Christiane Hader for her help with several experiments. A.G. was supported by a fellowship from the Jürgen Manchot foundation. Financial support was provided by the Krebsgesellschaft NRW.
AG and WAS conceived the study. GN was responsible for tissue acquisition. SJ performed the immunohistochemistry stainings and analyses. AG, JS, PN, and MJH carried out experiments and analysed data. AG and WAS wrote the paper. All authors had final approval of the submitted and published versions.


text:
Gerry Beckley "Unfortunate Casino"
Michael Ruff "You Are My Song"
King Kobra returns...
Veteran Hard Rockers King Kobra are back with a venomous new album Frontiers Records is excited to announce the release of the Self-Titled come back album from KING KOBRA on April 15th in Europe and May 5th in North America.
Formed in 1984 by legendary drummer Carmine Appice (Vanilla Fudge, Rod Stewart, Ozzy Osbourne), Los Angeles heavy metal outfit King Kobra recorded two critically acclaimed albums for Capitol Records – the 1985 release 'Ready to Strike' and 1986's 'Thrill of a Lifetime'.
Along with Carmine, the band featured four relatively unknown musicians at the time – David Michael-Philips and Mick Sweda on guitar, Johnny Rod on bass and Mark Free on lead vocals. King Kobra toured the U.S., Canada, Europe and Mexico playing shows with Kiss, Iron Maiden, Quiet Riot, Ted Nugent, Queensryche and Autograph.
The band was featured regularly on MTV and performed the theme song for the #1 1986 motion picture "Iron Eagle". After disbanding in 1988, each member continued his career in other successful major-label groups - Carmine to Blue Murder (Geffen), Mark Free to Unruly Child (Interscope), Mick Sweda to the BulletBoys (Warner Bros.), Johnny Rod to W.A.S.P. (Capitol/EMI) and David Michael-Philips to Lizzy Borden (Metal Blade).
The year 2010 saw the band reunite the original classic '85 lineup, replacing original singer Mark Free (now Marcie Free) with veteran lead vocalist Paul Shortino (Rough Cutt, Quiet Riot). "This album captures all the great points we had in the 80's, but better" says Carmine Appice "The music is more traditional King Kobra music. Great lyrics, kick ass songs with cool hooks and really good playing. A really great hard rock/metal record with lots of energy". David Michael-Phillips adds "It truly is an all-star album. Paul Shortino is amazing! I can honestly say he is one of the best vocalists in the business along with being one of the nicest and most generous people I have ever met".
King Kobra is smokin' hot, back stronger than ever and "Ready to Strike" once again!
Posted by Arnaud LEGER on February 19, 2011 in About new material | Permalink | Comments (0)

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July 4, 2016 - Like us now!
Microsoft is nagging its users one last time before the Windows 10 free upgrade offer expires on 29th of July. After the expiration, you will have to pay 12,000 PKR ($119) to upgrade to the latest operating system.
This notification is Microsoft's last try to get the users of Windows 7 and Window 8.1 to taste the latest operating system free of cost. We have been hearing woes and cries over the internet about automatic download and upgrade to Windows 10. Just recently, a woman won 10,000 dollars in a legal battle over a claim that Windows 10 was downloaded and upgraded her machine without her consent, causing her machines to slow down. Hence, losing business on the busiest time of the year.
The screen above shows you the kind of notification you will be getting from Microsoft's end. You can opt for a later reminder which will go on for 3 more times or you can upgrade right away. The machines which have blocked the notifications related to Windows 10 through 3rd party applications will not be getting this prompt. Also the ones, which had upgraded but reverted to the original operating system after a while.
If you are looking to upgrade to Windows 10 from Windows 7. It is going to be a palpable transition for you. After the Windows 10 upgrade, like over 300 million users you should be looking forward to the Windows 10 anniversary update which is bringing forth some new features and improvements.


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Important Notice Capacity is now at 100% but PHO still requires Covid Vaccine Passport with personal ID for entrance. Advance tickets at AGOG only please.
Sir is ostensibly a Mumbai-set romance about the sparks that fly between a servant and her well-to-do employer. But if you take away the slow-brewing attraction that serves as an appealing logline, you still have a mighty fine picture about a village girl who wants to defy the patriarchy and stand on her own two feet in the city. Now Magazine
Ratna – played with astonishing grace and immense feeling by Tillotama Shome – had been married off by her parents at 19 to a man who would die months later. According to village customs, her life should be spent mourning. Instead she takes a job in the city, escaping village scrutiny, while spending her wages on her little sister's education so that the latter doesn't succumb to a similar fate.
She also takes every free moment to learn tailoring, hoping to pursue a career in fashion design. That's what she's up to when she's not wiping down counters and preparing dishes for Ashwin (Vivek Gomber), a bachelor succeeding at his family's construction firm.
Ashwin comes from privilege but he's not as snide about it as the family and friends who surround him. He's a somewhat generic nice guy who has also been dealt a romantic blow in a relationship encouraged by his family. That is perhaps just one reason why he's attentive to Ratna.
Between them, a tender and observational class drama unfolds, one that avoids the most groan-worthy clichés. Writer/director Rohena Gera makes sure that Ratna, as humble as she is about her status on the social spectrum, isn't validated because of Ashwin and his affections.
While the film has a Cinderella set-up, and even recalls Pretty Woman at times, Ratna has a world-weariness about her that rebuffs fantasy. Even when a romance looks like it's on the horizon, Ratna remains guarded, knowing that Ashwin's doe-eyed sentiment isn't going to rescue her from her status and change how society sees her. She'd much rather stay invisible than throw herself into that drama.
What makes Sir so delicately thrilling is Shome's precise, heart-tugging performance: her posture, her eyes and the thoughts we assume are running through her mind as Ratna negotiates and processes whatever kindness or frustration is thrown her way. It's a monumental, awards-worthy performance. But since this is a humble independent Indian film, it will slip by completely unnoticed come awards season.
Perhaps that's how it should be. Ratna wouldn't have it any other way.
RADHEYAN SIMONPILLAI, NOW MAGAZINE


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The 2014–15 St. Louis Blues season was the 48th season for the National Hockey League franchise that was established on June 5, 1967.

Off-season
Coach Ken Hitchcock was extended through this season on May 7, 2014, as were Associate Coach Brad Shaw and Assistant Coach Ray Bennett. Assistant Coach Gary Shaw and Goaltending Coach Corey Hirsch were not retained. Kirk Muller, 48, was named assistant coach by General Manager Doug Armstrong on May 13, 2014. Muller was formerly head coach of the Carolina Hurricanes in parts of the last three seasons.

Goaltender Brian Elliott re-signed for a three-year extension on May 19, 2014, for $7.5 million. He will team with Jake Allen. Ryan Miller will become an unrestricted free agent in July.

The Blues on May 27, replaced their unretained goaltending coach with Jim Corsi, formerly goaltending coach of the last 16 seasons with the Buffalo Sabres.

Playoffs

The St. Louis Blues entered the playoffs as the Central Division regular season champions. The Blues took on the wild card Minnesota Wild  in the first round. The Blues were defeated in six games by the Wild.

Standings

Schedule and results

Pre-season

Regular season

Playoffs

Player statistics
Final Stats

Skaters

‡Traded away mid-season, date in ( ). Stats reflect time with Blues only.
†Denotes player spent time with another team before joining Blues, acquired date in ( ). Stats reflect time with Blues only.
Bold = leading team in category.

Goaltenders

Awards and honours

Awards

Milestones

Transactions
Following the end of the Blues' 2013–14 season, and during the 2014–15 season, this team has been involved in the following transactions:

Trades

Free agents acquired

Free agents lost

Lost via waivers

Lost via retirement

Player signings

Draft picks

The 2014 NHL Entry Draft will be held on June 27–28, 2014 at the Wells Fargo Center in Philadelphia, Pennsylvania.

Draft notes
Edmonton's second-round pick will go to St. Louis, as the result of a trade on July 10, 2013 that sent David Perron to Edmonton, in exchange for Magnus Paajarvi, and this pick.
 The Calgary Flames' fourth-round pick went to the St. Louis Blues as the result of a trade on June 28, 2014 that sent Roman Polak to Toronto in exchange for Carl Gunnarsson and this pick.     Toronto previously acquired this pick as the result of a trade on September 29, 2013 that sent Joe Colborne to Calgary in exchange for this pick (being conditional at the time of the trade). The condition – If Calgary fails to qualify for the 2014 Stanley Cup playoffs then this pick will remain a fourth-round pick in 2014 – was converted on March 30, 2014.
Tampa Bay's fourth-round pick will go to St. Louis, as the result of a trade on July 10, 2012 that sent B. J. Crombeen and a fifth-round pick in 2014 to Tampa Bay, in exchange for a fourth-round pick in 2013, and this pick.
St. Louis's fourth-round pick will go to the Nashville Predators, as the result of a trade on June 30, 2013 that sent a fourth-round pick in 2013 to St. Louis, in exchange for a seventh-round pick in 2013, and this pick.
Calgary's fifth-round pick will go to St. Louis, as the result of a trade on July 5, 2013 that sent Kris Russell to Calgary, in exchange for this pick.
St. Louis's fifth-round pick will go to the Tampa Bay Lightning, as the result of a trade on July 10, 2012 that sent fourth-round picks in 2013 and 2014 to St. Louis, in exchange for B. J. Crombeen and this pick.
Boston's sixth-round pick will go to St. Louis, as the result of a trade on April 3, 2013 that sent Wade Redden to Boston, in exchange for this pick (being conditional at the time of the trade). The condition – St. Louis will receive a sixth-round pick in 2014 if Redden appears in one playoff game in the 2013 for the Bruins – was converted on May 1, 2013.

References

St. Louis Blues seasons
St. Louis
St. Louis Blues season, 2014-15
St. Louis Blues
St. Louis Blues

redpajama_set_name: RedPajamaWikipedia


text:
首页 » Damona » 2019上海新后宫阁
Tag: 2019上海新后宫阁
Participants to the forum also expressed willingn
gness to actively take part in the construction of the pilot zone, and work together to
eradicate the barriers in terms of policy, technology and trade, said the document.
All sides are full of expectations for facilitating policies, which will allow new technologie
s and new products that conform with international standards to enter the pilot zone, said the declaration.
They are also ready to offer assistance to this initiative within their capabilities, and expect
the pilot zone to provide a new platform for medical innovation and cooperation in the region, it said.
The "China Shanghai Cooperation Organization (SCO) Research Center for Medi
cal Innovation" will also be established in Fangchenggang, according to the declaration.
www.fywmgz.cn
We must work together to ensure that big data, and the
e technologies that it enables, are harnessed for the benefit of mankind while minimizing the risks to devel
opment, peace and security and human rights," Guterres said in a congratulatory letter to the expo.
Miao Wei, minister of industry and information technology, said China has already made sig
nificant progress in bolstering the big data industry with a string of big data platforms established in sect
ors such as manufacturing, commerce, finance, transportation and medical care.
"We will make a fresh push to integrate cutting-edge information technologies into the re
al economy, including establishing a national industrial data center, to better power the country's sp
rawling manufacturing sector," Miao said at the opening ceremony of the big data expo.
www.sdnxs.org
hina plans to boost rural development via digital technologies
China plans to promote the applications of digital technologies in rural areas to boost local development.
As a step towards rural modernization and transformation, digital rural development play
s a crucial role in rural revitalization and building a digital China, according to the guideline jointly issue
d by the general offices of the Communist Party of China Central Committee and the State Council.
The country has vowed to achieve initial development of buildin
g digital villages by 2020, with 4G internet accessible to more than 98 percent of the adm
inistrative villages and the digital economy growing rapidly in rural regions, the guideline said.
With the upgrading of internet technology, 2025 will see a n
otable narrowing of the urban-rural digital gap, the establishment of various ent
repreneurial and innovation centers in rural areas, and an intellectual rural logistics system.
www.fqyp.org
The supply of high-end talent, government investmen
t in culture and education, attractiveness for foreign investment and consumption scale are also indicators under consideration.
Shanghai has the highest infrastructure and cultural environment indexes, and the financi
al hub's talent, technological innovation and financing indexes are in the top three of the country.
Although Beijing's soft environment index ranked first among all Chinese cities, its hard environment index lagged behind
due to restriction of natural resources such as air quality, climate and forest coverage rate, the report said.
With the best hard environment, South China's Shenzhen also leads on the country's technological innovation index.
Roads and buildings were illuminated Tuesday evening befor
I hope all Asian countries will respect and trust each other, co-exist in harmony, expand cross-border, cross-time-and-space as well as cross-civ
ilization exchanges, and jointly maintain the peaceful time that is more valuable than gold.
Asian people expect Asia of common prosperity
I hope all Asian countries will jointly promote economic globalization that is open, inclusiv
e, balanced, and beneficial to all, and work together to eliminate poverty and backwardness in some countries.
Asian people expect open, integrated Asia
Civilizations will lose vitality if countries go back to isolation and cut themselves off from the rest of the world.
Mutual respect, equal treatment among civilizations stressed
It is foolish to believe that one's race and civilization are superior to
others, and it is disastrous to willfully reshape or even replace other civilizations.
www.nqmqgh.cn
Often these pictures do not represent how these indivi
duals look in real life without the posing, the filters, the photoshopping and the professional hair and make-up," Martz said.
Pan, the Renmin University researcher, also believes that pi
ctures shared frequently on social media impose peer pressure on women.
"If people around you look thin on WeChat Moments, you are inevi
tably anxious to fit the general profile of having the 'right look'," she said.
Articles have also gone viral in summer on WeChat Moments, with sensational headlines such as "Good-looking girls never
weigh more than 50 kilograms" or "If you don't lose weight in May, you will cry out loud in June".
Shen Zijiao, a psychological consultant at Beijing Normal University, said: "Women don't know what kind of beauty is best for th
em; they just thumb through their phone screens and get the notions of 'being slender' consciously or uncons
ciously. They always worry if their bodies are inconsistent with the so-called perfect body shape."
ever-spring.org
teps aimed at helping further develop countryside opt
hina will undertake key measures to further integrate the country's rural and urban areas
and encourage the transfer and free flow of people and resources throughout the nation.
Measures include relaxing hukou (household registration) barri
ers, reforming zoning regulations for rural and residential land, marketing collective land
in rural areas for development purposes and increasing infrastructure spending in the countryside.
Experts said the new plan, announced by the Communist Party of China Ce
ntral Committee and the State Council late on Sunday, is aimed at helping realize steady progr
ess in rural revitalization, driving up the process of urbanization and further boosting the economy.
China has a three-step plan. First, it will initially build a framework an
d mechanism for integrated development of urban and rural areas, and gradually rem
ove limits on hukou registration in cities by 2022. Second, it will bring about an improved framewo
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Boosting rural areas will help solve dilemmas of ove
rcrowding and rampant construction plaguing cities, and the development of cities will also offer unique ways to bring about rural revitalization," Chen said.
"As restrictions on hukou will gradually be removed, cities need to be well-prepared to offer
accommodation and employment opportunities, and allow children of migrant workers to have equal access to education," Chen added.
China has made steady progress in urbanization, as the ranks of permanent urban r
esidents stood at 831 million at the end of 2018, up 17.9 million from the previous year, said the National Bureau of Statistics.
Last month, the National Development and Reform Commission said it
plans to increase the urbanization rate by at least 1 percentage point by the end of this year.
Shen Chi, vice-director of the China Center for Urban Development, said the government's new
plan will help foster high-quality and sustainable economic development across the nation.
"Relaxing the hukou policy will be a key step in promoting the free flow of labor across
the nation," Shen said. "A systematic consideration and arrangement of the integratio
www.oxkinp.cn


text:
Paradactylopodia simillima är en kräftdjursart som först beskrevs av Brady 1910.  Paradactylopodia simillima ingår i släktet Paradactylopodia och familjen Thalestridae. Inga underarter finns listade i Catalogue of Life.

Källor

Hoppkräftor
simillima


text:
Switzerland competed at the 2014 Winter Paralympics in Sochi, Russia, held between 7–16 March 2014.

Medalists

Alpine skiing

Men

See also
 Switzerland at the Paralympics
 Switzerland at the 2014 Winter Olympics

References

Nations at the 2014 Winter Paralympics
2014
Winter Paralympics


text:
Items tagged with PlayStation-4
by Brittany Goetting - Thu, Oct 21, 2021
God Of War In 4K Rides Victorious To PCs On Tears Of PlayStation Elitists, Pre-Order Now
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Last week, Sony teased the next generation of PlayStation VR with DualSense technology, which got many gamers excited. As that new headset and technology is still on the horizon, current PSVR gamers want new games to play with their current hardware. Today, Sony brought back the PSVR Spotlight to announce six new... Read more...
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Sony today released Software Update 8.00 for the PlayStation 4, which included a bunch of new social features. Sony also stealthily added a voice "Party Safety" notification letting users know their voice chat may be recorded. Some gamers were taken aback by this and were quick to call it out, and Sony subsequently... Read more...
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Sony Celebrates Days of Play Sale With Limited Edition Blue PS4
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Detroit Become Human Reviews Offer Praise For Futuristic Android Drama With Gameplay Choice
What kind of video game elicits multiple play-throughs? Quantic Dream's latest video game Detroit: Become Human offers players a variety of dialogue choices that can deeply affect the lives of characters in the game. Detroit: Become Human was released worldwide yesterday and has received generally favorable reviews... Read more...
by Shane McGlaun - Fri, Apr 27, 2018
PS4 Shipments Reach 79 Million As Sony's Gaming Success Soars
Sony this week gave up some details on its gaming business while presenting its financial results for the recently completed quarter. The highlight of the announcement was that Sony shipped 19 million PS4 consoles for fiscal 2017. That is about a million its less than it shipped in fiscal 2016, but it was enough to... Read more...
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Sony Slashes PlayStation VR Headset Prices For A Limited Time Starting This Weekend
If you have been kicking yourself for missing out on the deals that were offered on the PlayStation VR during the holiday season, you can make it up to yourself this weekend. Sony has announced that it will be offering $100 off the price of the PlayStation VR standalone headset, Doom VFR Bundle, and the Skyrim VR... Read more...
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PlayStation 4 System Software Update 4.5 Arrives Tomorrow With PS4 Pro 'Boost Mode'
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AMD Q4 Sees Strong 2013 Revenue Growth In GPUs and Console APUs, PC Financials Weak
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Bungie Announces 'Destiny' Release Date, Mark Your Calendars
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by Seth Colaner - Wed, Oct 23, 2013
Battlefield 4 Single Player Story Trailer Impresses With Characters, Storyline, And Visuals
The latest Battlefield 4 game trailer from EA is here, and this one is a single-player story trailer that gives you a taste of the sort of character arc you can expect from the game. The trailer starts out with the evacuation of American VIPs from Shangai and--wouldn't you know it--things get a little crazy on... Read more...
by Seth Colaner - Sun, Oct 13, 2013
Skylander Swap Force Blockbuster Adventure Kids Game Ships Today Mate!
If you've never played "Skylanders" with kids before, you're missing out. It's easy to see why the franchise is so popular; the game is simple enough that young kids can play it, yet it's sufficiently entertaining that you don't get bored playing it with them, and the... Read more...
by Seth Colaner - Tue, Sep 17, 2013
EA's Battlefield 4 Multiplayer Gameplay Trailer Will Leave You Drooling
We love a good gameplay trailer around here, and the latest one from EA showing off Battlefield 4's multiplayer play looks fantastic. We already saw a lengthy (17 minutes) demo earlier this year that gave us a look at the fluid and detailed visuals as well as the storyline with a mix of scripted scenes and... Read more...
Sony Unveils PlayStation Vita TV Low-Cost Mini Console Streamer
Sony made several announcements about new and updated products, including confirming that the U.S. release of the PlayStation 4 is November 15th and that the mobile PlayStation Vita is getting a refreshed look, but the big news concerned a small streaming micro console called the PlayStation Vita TV. The device has... Read more...
by Seth Colaner - Mon, Sep 02, 2013
Sony PlayStation 4 To Support Voice Control And Facial Recognition
Sony's upcoming PlayStation 4 will support voice commands and facial recognition through its PlayStation 4 Camera peripheral. Unlike the Microsoft's Kinect camera, which is standard issue with the Xbox One, Sony's camera will be sold separately--or taken another way, optionally. A Sony Entertainment... Read more...
by Seth Colaner - Wed, Aug 14, 2013
Catch The Call of Duty Ghost Multiplayer Live Stream Here at HH!
Want to know everything there is to know about the new Call of Duty? Bang it here for the live stream of the full reveal of Call of Duty: Ghosts. Call of Duty: Ghosts will be available for the PC as well as the Xbox One, Xbox 360, PlayStation 3, and PlayStation 4 consoles. It appears to be coming for the Wii U, as... Read more...
by Seth Colaner - Tue, Jun 25, 2013
Sony Pummels Microsoft Again: More Exclusives Coming To PS4 Than Xbox One
Ever since Microsoft announced the Xbox One, the company has been dealing with a good bit of blowback for some of the features of the new console, including the requirement for an online "check in" every 24 hours; a required, always-on Kinect (that costs an extra $100 to boot); and the inability to sell... Read more...


Red Mill House Bed and Breakfast is famous for friendly service, excellent local information and home-made breakfasts. There are also birdwatching tours and guiding available.
Beautiful gardens, large swimming pool, wide verandahs, laundry and free internet compliment the spacious rooms and friendly service.
Four spacious ensuite rooms with queen, king or twin beds. Tastefully decorated with large bathroom, air-conditioning and large veranda overlooking the tropical gardens. Tea and coffee making facilities and fridge. Cot or rollaway bed available. Communal lounge and reference library.
Two bedroom family unit in historic Red Mill House. It has a separate lounge, utility area, bathroom and toilet. One bedroom has twin beds, the other has a queen bed. Suitable for a family of four or for three adults.
The unit has direct access to the garden. There is a communal lounge and verandah upstairs.


Amazon: Is the threat to your business as big as it's made out to be?
It's named after the largest river in the world, and its ambitions are just as big. It is sometimes hard to remember that "the everything store" began as a mail-order book store. These days, there doesn't seem to be a part of our lives that Amazon isn't part of, or trying to become a part of: from our groceries to our TV programmes and now healthcare, as announced on 28 June. The news for other, smaller (and there are very few businesses that aren't smaller!) businesses can seem bleak.
But Greenlight's research, compiled from over 200 digital marketers, paints a different picture. Only 32% of respondents say they have lost sleep worrying competition from Amazon will impact their business in the next 18 months. Of course, no-one is dismissing Amazon's reach and power, but as our survey shows, despite the dire warnings in eCommerce publications and blogs, there are many people who aren't that concerned. Or rather people are looking closely at ways to remain competitive, despite, or in some cases, because of Amazon.
Smaller businesses are using Amazon as a springboard to launch into international markets – 38% of those surveyed thought this would be a way their company could work with Amazon. New balance, for example, is using the tech giant to provide international next-day delivery through their Prime service. Rather than being intimidated by Amazon's size and infrastructure, retailers are utilising Amazon's own logistics, by using them as a way to reach new sectors, or to try out new categories.
By using Amazon's logistics, smaller businesses can ship items to Amazon's warehouses and then make use of Prime delivery. In this way, smaller businesses are able to offer their customers the same value proposition as Amazon itself, without the initial investment.
It's also helping brands such as Casio to fight against counterfeit items and brand misrepresentation though the Amazon Brand Registry.
Equally, some companies have made the most of their differences with Amazon in order to compete with it. Ironically, automation, the very thing which has allowed Amazon to grow so quickly and efficiently, has also given it its greatest weakness – very poor customer service. 39% of respondents in our recent survey believed that providing better customer service was the best way to compete against Amazon.
No matter how sophisticated the technology, people still like to buy from people. Businesses need to have a physical face, a customer needs to feel that there is a real-life person out there who can help them. No-one wants just automation – we can see clearly that despite huge technological advances, customers still want that personal touch. People want to touch and feel before purchasing certain products. There is a whole shopping process that people still expect and enjoy, including store locations, a personal experience, human interactions.
Amazon Prime aims to make customers' lives easier in most areas, but pre-sale it offers very little service apart from user-generated reviews. Trust of these however has been hugely damaged by the rise in fraudulent reviews. For companies who are happy to offer better customer service (or any customer service!), this can be a hefty advantage.
Lush is consistently ranked as one of the top brands for customer experience. One of the ways they do this is training their staff to recognise different customer types and tailoring their behaviour to that e.g. remembering customer names and interests of "amiable" customers. They also empower their staff and give them the freedom to go the extra mile.
As with automation, so with scale – in some respects, Amazon's immense size is as much of a weakness as it is a strength. For many retailers, Amazon simply sells too much stuff, and it is impossible to stand out from the crowd on that platform.
A large concern for several companies is Amazon taking over certain sales days. Our research shows that this was in fact a concern for 37% of respondents, more of a worry than Amazon's competitive pricing (which concerned 33% of respondents) and logistical efficiency (31% of respondents). However, many brands want to be a destination, not a sales brand. By deciding not to sell with Amazon, they can re-position themselves effectively.
LVMH is on record to say they would not work with Amazon. It could be suggested that part of the reason for this is to protect their brand by being more exclusive and controlling of how their products are merchandised.
There is no denying the impact Amazon has had on the retail industry, and with its moves into B2B, food delivery, grocery and now healthcare, there is still reason to be nervous. But as we have shown, for other companies, there are still plenty of options and advantages, and hopefully now fewer sleepless nights!


The Raspberry Pi foundation have posted news of their new 8 mega pixel camera which was made available today. This was followed by members of the Pi in the Sky project confirming compatibility. So there will be even better SSDV images from near space for those people flying a PITS board.
The 5-megapixel visible-light camera board was our first official accessory back in 2013, and it remains one of your favourite add-ons. They've found their way into a bunch of fun projects, including telescopes, kites, science lessons and of course the Naturebytes camera trap. It was soon joined by the Pi NoIR infrared-sensitive version, which not only let you see in the dark, but also opened the door to hyperspectral imaging hacks.
As many of you know, the OmniVision OV5647 sensor used in both boards was end-of-lifed at the end of 2014. Our partners both bought up large stockpiles, but these are now almost completely depleted, so we needed to do something new. Fortunately, we'd already struck up conversation with Sony's image sensor division, and so in the nick of time we're able to announce the immediate availability of both visible-light and infrared cameras based on the Sony IMX219 8-megapixel sensor, at the same low price of $25. They're available today from our partners RS Components and element14, and should make their way to your favourite reseller soon.
In our testing, IMX219 has proven to be a fantastic choice. You can read all the gory details about IMX219 and the Exmor R back-illuminated sensor architecture on Sony's website, but suffice to say this is more than just a resolution upgrade: it's a leap forward in image quality, colour fidelity and low-light performance.
VideoCore IV includes a sophisticated image sensor pipeline (ISP). This converts "raw" Bayer-format RGB input images from the sensor into YUV-format output images, while correcting for sensor and module artefacts such as thermal and shot noise, defective pixels, lens shading and image distortion. Tuning the ISP to work with a particular sensor is a time-consuming, specialist activity: there are only a handful of people with the necessary skills, and we're very lucky that Naush Patuck, formerly of Broadcom's imaging team, volunteered to take this on for IMX219.
Regarding the tuning process, I guess you could say the bulk of the effort went into the lens shading and AWB tuning. Apart from the fixed shading correction, our auto lens shading algorithm takes care of module to module manufacturing variations. AWB is tricky because we must ensure correct results over a large section of the colour temperature curve; in the case of the IMX219, we used images illuminated by light sources from 1800K [very "cool" reddish light] all the way up to 16000K [very "hot" bluish light].
The goal of auto white balance (AWB) is to recover the "true" colours in a scene regardless of the colour temperature of the light illuminating it: filming a white object should result in white pixels in sunlight, or under LED, fluorescent or incandescent lights. You can see from these pairs of before and after images that Naush's tune does a great job under very challenging conditions.
As always, we're indebted to a host of people for their help getting these products out of the door. Dave Stevenson and James Hughes (hope you and Elaine are having a great honeymoon, James!) wrote most of our camera platform code. Mike Stimson designed the board (his second Raspberry Pi product after Zero). Phil Holden, Shinichi Goseki, Qiang Li and many others at Sony went out of their way to help us get access to the information Naush needed to tune the ISP.
We're really happy with the way the new camera board has turned out, and we can't wait to see what you do with it. Head over to RS Components or element14 to pick one up today.


Healthy N Fit: Home Remedy - Vomiting !!!
Vomiting or vemesis is a biological defense mechanism. It is a reflex activity, which refers to the forceful oral expulsion of stomach contents. The actual function of vomiting is to remove toxic or harmful substances from the body after ingestion. However, vomiting is multifactorial in origin and can be caused due to various reasons. Retching is the sustained contraction of the chest and abdominal muscles that accompanies before or during vomiting.
Heat 2 cardamoms on a dry tava. Powder them and add a tsp honey and take frequently.
Take a mixture of 1 tsp mint juice with 1 tsp lime juice and 1/2 tsp ginger juice along with a tsp honey.


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Q: Dead Battery Voltage and its Charging
*

*How can we say a battery,say Li-ion battery, is dead when it is 2.8V itself instead of 0V? How can the 2.8V not give current? What is happening? This question, is applicable to all other batteries also. Why do we say a battery is dead when it is not completely 0V, but when it is hanging some very around 2.8V for Lithium ion and 1.1V for the AA battery whose nominal voltage is 1.5V and others?

*I have a Li-ion battery which I tried to charge. The maximum voltage mentioned in the battery is 4.4V max. And its nominal voltage is 3.85V. But, when I connect the battery to the USB Charger to charge the dead battery which was at 2.8V, the voltage line reads 4.6V. How is the 4.6V obtained and won't the battery get damaged as we are feeding 4.6V which is more than its maximum 4.4V voltage which is mentioned?

A: Short answer: Think of your dead battery as the same battery but with very very reduced capacity. If you leave it alone, it shows some voltage. But as soon as you plug it into a device to extract current from it, it gets empty within milliseconds. When you try to charge it, it appears to fill up quickly.
Discussion: There are many failure modes for each type of battery, some leave them at 0V, some cause them to have high internal resistance, some leave them with reduced charge capacity, some make them leaky. These failure modes can occur alone or simultaneously. The two indications that you mentioned, both seem to be caused by a battery having too little capacity.
But even for a good lithium ion battery, the amount of charge available in the battery is not proportional to it's voltage. An Li ion battery delivers most of its charge between 3.4 V and 4.2 V. Below this voltage, it does have some charge, and it will definitely supply current, but its voltage will fall towards zero very quickly if you draw current from it.

redpajama_set_name: RedPajamaStackExchange


Coconut oil is becoming extremely popular because of its health benefits. Reduced instances of heart attack, cancer, and colon disorders are observed in people, who are integrating it into their daily diet. All these health benefits may make you want to try out these recipes.
Coconut is easily available in the tropical areas and so is its oil. The oil is extracted from dried coconut and has many health benefits. It does not contain any saturated fats, which makes it beneficial for patients suffering from heart ailments, diabetes, and high-blood pressure. Lauric acid, caprylic acid, and capric acid are its important constituents. These acids make it helpful in dealing with many viruses and bacteria. It is usually recommended for making food because it has lesser calories and the oil is easily converted into energy by the human body. Let's get to know some of the delicacies made from it.
Mix oats, oat bran, almonds, and coconut. Bake this mixture in a preheated oven at 350° F. The oats are kept in the oven for 7-8 minutes, till they become crisp. In the meantime, combine coconut milk, oil, malt syrup, and honey in a cooking pan. Boil this mixture for 1-2 minutes and wait till the mixture comes to room temperature. Add vanilla extracts to this syrup.
The granola in the oven is cooled and the syrup is added, until it gets fully coated. Spread this coated granola on the baking sheets and toast it in a preheated oven for about 8 minutes. Let it cool down and then add cranberries with the crisp granola. Use an airtight container to store the cooled cereal. Dried pineapple or dates, instead of cranberries, can be added to get a different flavor.
Organic Virgin Coconut Oil, 2 tbsp.
Cook celery, cauliflower, and onion in water for about 20 minutes on a low flame, to make them tender. Blend the mixture in a blender at low speed. Heat some oil in a saucepan and add flour to it. Stir the flour frequently, until it turns brownish in color. Add coconut milk and stir the flour, till the mixture becomes even.
Combine the puree from the blender with the flour mixture in the cooking pan. Add curry powder, salt, and pepper. Stir till it becomes hot; but do not boil it. A healthy soup is ready.
Cut the chicken in small portions. Heat oil in a frying pan and fry the chicken pieces until they turn brown and crunchy. Keep this fried chicken aside. Sauté onion, garlic, and mushrooms for 3 minutes in the same frying pan. Put the fried chicken in the pan and add coconut milk, and pepper to it.
Take a cheese pouch and tie hot pepper, thyme, and parsley in it to make a bouquet garni. Add this to the chicken curry in the frying pan. Cover it and simmer till it becomes tender. Remove the bouquet garni, after simmering the chicken for 1¼ hours and serve it with boiled rice.
Mix the coconut oil and milk in a mixer. Then blend this mixture with the other ingredients and serve.
Dishes made with this oil are healthy and help to reduce weight. Including it in your daily diet is the easiest way to increase vitality and energy.


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Optipress shares dip following IPO
DARTMOUTH, NS—Canada's newest printing company, Optipress, completed its IPO of 7 million common shares at $8.50 each on Thursday. Optipress is now listed on the Toronto Stock Exchange under the symbol OPP and its shares are publicly traded. At close of trading last night, the share price had droped to $8.35 and no shares of the company were traded yesterday. In accordance to the terms of the underwriting agreement, Newfoundland Capital Corp. purchased 1.41 million shares of the company and Cameron puchased 588,200. Optipress will now purchase the printing and publishing assets of NCC and Cameron Publishing.
Sales down slightly for Quebecor
MONTREAL—The largest printer in the land is reporting sales for the second quarter that are only slightly below the results from one year ago. Quebecor World announced yesterday that sales for the quarter ended June 30, were US $1.47 billion, just a smidge below the US $1.50 billion from the same time in 2001. Revenues for the first six months of the year are down 5% compared to a year ago. Downward pressure on sales continued to come from lower demand for print advertising and promotion and price pressure due to excess capacity. Net income for the quarter was up 4%, a sign that its restructuring efforts are paying off, the company says.
MM&T buys Ashton Potter
MISSISSAUGA, Ont.—McLaren, Morris & Todd (MM&T) bought MDC's Ashton Potter folding carton operation in Mississauga earlier in July. MM&T is a subsidiary of U.S.-based Mail-Well Inc. The acquisition has been renamed MM&T Packaging and is currently headed by Al George who said the purchase rounds out the services the company can offer and completes the value-added offerings to customers. Plans are to run the plant independently of MM&T and the deal does not include Ashton-Potter's stamp-printing operation in Williamsville, N.Y. Gord Griffiths, president of the Mail-Well print group, said the company has no immediate plans for more acquisitions here but said that "the Canadian marketplace is always of interest to us." This is the second high-profile divestiture for MDC, which earlier this year sold its cheque-printing division, Davis + Henderson. The company, which did not return phone calls, now has only two operations left in Canada, Metaca card printer in Ont., and Mercury Graphics ticket printer in Sask.
Sales down, profit up at Moore
STAMFORD, Conn.—Moore Corporation reported reduced sales for the second quarter ended June 30, but higher net earnings. Sales came in at US$499.9 million, down from US$532.5 million recorded in the second quarter last year. According to the company, the revenue decline was the result of some fourth quarter divestitures, including Phoenix and some Forms and Labels business. Net earnings for the period totalled US$15.2 million which compares favourably to last year's loss of US$60.4 million after restructuring and other non-recurring charges.
Ernie Bardocz leaves Heidelberg
MISSISSAUGA, Ont.—PrintCan has learned that Ernie Bardocz, long-time employee of Heidelberg Canada, has resigned from the company to pursue other interests. He left the company on July 15, after 18 years in various sales and marketing positions.
Ryerson graphics centre nearing completion
TORONTO—Ryerson's Centre for Graphic Communications Management (GCM) is in its final stages of construction. Mary Black, chair of the school of GCM, says "It's coming along extremely well and on schedule. We expect to be in it by September." She says some exterior masonry work still needs to be done and cosmetic interior painting and decorating will take place over the rest of the summer. The $10 million, 30,000 sq. ft. complex will house more than 350 GCM students in the fall. PrintCan has also learned that a grand opening ceremony will take place in October. Although no plans have been finalized and a date has not been announced, Black says she wants the industry to be involved.
Transcontinental prints 300,000 gospels for World Youth Day
LOUISEVILLE, Que.—Despite a tight schedule of about two weeks, Imprimerie Gagné, a division of Transcontinental, managed to crank out 300,000 Gospels of Matthew for the 17th World Youth Day being held in Toronto next week. Printed on one of the company's web presses, the pocket-sized book measures 3.5" x 5" and contains four languages. A similar project would normally take five or six months. According to Denis Audet, general manager at Imprimerie Gagné, the run was actually cut by more than half from one million copies. "We were planning to split the job with Best Book in Peterborough because we have the same types of presses and services, but the run came down." Nelly Safari, production manager at The Canadian Bible Society, says "there were a lot of glitches along the way, but for a project of this magnitude, it was expected." A major problem was matching the colours of the cover image to an oil painting depicting St. Matthew and The Angel. Safari says proofs were sent back and forth several times.
Optipress goes forward with IPO
DARTMOUTH, NS—Optipress has received regulatory approval to go ahead with its IPO. The offering consists of 7 million common shares at $8.50 each. The offering is expected to be completed by July 25 and net proceeds will be used to buy the publishing and printing assets of Cameron Publications and Newfoundland Capital Corp. Contrary to a previous announcement that NCC would not own any shares of the new entity, the underwriting agreements states that it has agreed to purchase 1.41 million shares of Optipress if requested by the underwriters. According to a press release, Optipress also intends to consolidate existing printing operations in Newfoundland, Nova Scotia, New Brunswick and New England.
Hemlock president a finalist for Entrepreneur of the Year award
BURNABY, BC—Dick Kouwenhoven, president of Hemlock Printers, is in the running for Ernst & Young's 2002 Pacific Entrepreneur of the Year award. He is a finalist in the manufacturing division and joins a list of 17 other nominees in six categories from the West Coast. A winner will be named in each category and the six will vie for the Pacific Entrepreneur of the Year award. The winner of the Pacific competition will be considered for the national Entrepreneur of the Year award. "It's an incredible honour," Kouwenhoven says. "Not only will this be good for myself, but it also reflects well on the company." Hemlock was established in 1968 and specializes in fine art books and multi-colour annual reports. Hemlock was also recently included in the top 100 private companies in B.C. by Business in Vancouver newspaper, coming in at number 92. Last year the company achieved annual sales of of $35.5 million.
Transcontinental purchase includes two printing plants
MONTREAL—Two web printing plants, Ad Venture in Saskatoon, and Williams & Crue in Summerside, P.E.I., changed hands when GTC Transcontinental bought a group of 12 community newspapers and related publications from CanWest Global Communications on Wednesday. The deal is expected to generate $100 million in annual revenue; printing will account for about $10 million of that, says Luc Desjardins, president of Transcontinental Printing. All the newspapers acquired are already being printed at the Saskatoon and Summerside locations or have in-house printing facilities, so there will be no moving of printing contracts to Transcontinental plants. As a result of the Williams & Crue purchase, Transcontinental also takes over printing of the National Post in the East Coast. It is now the printer of both national newspapers in the region.
Tyrell Press files proposal to creditors
GLOUCESTER, ON—PrintCan has learned that commercial printer Tyrell Press has run into financial turbulence. The Gloucester, Ont., company has filed a notice of intention to make a proposal to its creditors. It is expected that it will develop a debt repayment plan within the next 30 days. When contacted, the company confirmed its bankruptcy status but had no further comment. D&A MacLeod Co. Ltd. is the company's trustee but was not available for comment at this time. Tyrell Press has been in business for more than 40 years. Stay tuned for more details.
Former Arthurs-Jones partner moves to Peel Graphics
BRAMPTON, Ont.—Casper Stabile is on the move again. He has recently moved to a sales position at Peel Graphics in Brampton. Stabile was one of three partners at Arthurs-Jones Clarke, until it declared bankruptcy last summer. He then moved on to a sales position with Spinnaker Graphics in Mississauga, which PrintCan has learned he left about a month ago. Spinnaker would not comment on his departure and Stabile was not available for comment at this time.
Schawk acquires Mississauga prepress company
TORONTO—After months of industry rumblings, it has been confirmed that Schawk Canada has purchased Imaginex, adding to the number of its existing Toronto locations. Bob Cockerill, president of Schawk Canada, says the acquisition was a natural choice for the prepress company that specializes in flexography. "It (Imaginex) is strong in flexo and flexo platemaking, so it was a keen interest to us," he says. Similar to other Canadian Schawk locations, Cockerill says Imaginex will probably be renamed Schawk Mississauga. Although he couldn't disclose the sale amount, Imaginex reported revenue of $16.5 million in 2001.
Imaging Excellence completes printing rights case
TORONTO—After six years of court hearings and appeals, the case between Imaging Excellence and the Canadian Lesbian and Gay Archives (CLGA) is finally complete. In late June, a three-judge panel of the Ontario Superior Court ruled that work proselytizing or promoting the gay and lesbian lifestyle would conflict with owner Scott Brockie's freedom of conscience and faith and would be within his rights to refuse. Brockie says he is still obligated to pay the $5,000 penalty. And although Brockie admits it is only a partial victory, he says "there is now some protection to refuse some jobs that offend your core beliefs." It all began back in April of 1996. Brockie, a Born Again Christian, refused to print the CLGA's letterhead and brochures saying he objected to their views on acceptable sexual practices. "The thing kind of took on a life of its own," he says of the case. He says both parties have accepted the decision and no further appeals will be made. Meanwhile, Imaging Excellence is still undergoing a financial restructuring plan after appointing trustees in April. A proposal was sent to creditors that Brockie says was almost unanimously accepted. "It's nice to see this stuff behind us so we can carry on with what really counts and that's running the business."
Printer loses contract to Internet
AURORA, Ont.—Ricoh Document Management, a subsidiary of Ricoh that provides document and print services, has lost a print contract after 11 years of printing. The Toronto Stock Exchange's Daily Record, a 40-page summary of stock market information, has been moved to cyberspace and will be delivered via email. Alan Wheeler, vice president and general manager of the Aurora printer, says it was a relatively small contract and is not worried about losing further work to the Internet. "People don't read things in any volume off the screen so, ultimately, it is going to be printed somewhere," he says. Steve Kee, director of media relations of the TSX Group, says speed was the deciding factor for the change. A printed copy could take up to two days to reach a subscriber.
Quebecor World dismantles Reader's Digest press
MONTREAL—After 27 years of printing Reader's Digest on a Baker Perkins G-12 press, which the publication owned, Quebecor World has dismantled the old press and is printing the magazine on its own web offset machines. "It started to cost us major money in repairs and the quality was slipping to a point where we didn't want it to go any further," says Linda Melrose, production manager at Reader's Digest. Quebecor World has printed the magazine on presses owned by Reader's Digest Magazines Ltd. (first a Goss then the Baker press) since the company set up shop in Canada in 1947. However, the publication has no plans to purchase another machine for printing. "The presses now are so fast, we couldn't use all the hours," Melrose says.
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