This is my fifth year of teaching and one of the things I have observed with surprise and relish is the number of experienced teachers who, despite the difficult and demanding realities of the teaching profession, still maintain and strive to share their love of teaching and learning, and their passion for the subjects and students they teach. It is my belief that a teacher's passion for teaching is key in the art of juggling everyday demands while still remaining motivated and empowered, allowing them to be the best teacher they can be. This zest, and the belief in the value of teaching and learning, is something that I intend to remind myself of throughout my career in order to remember my motivations for becoming a teacher and maintain my enthusiasm. This website is a way to keep that fire burning and share my passion for teaching and technology with other like-minded educators, as well as reflect on and evaluate my practices and resources.
A love of teaching and learning and a desire to instill that love of learning in our students is the most basic motivation of most teachers, but I believe the relationship between teacher and student should be reciprocal. The teacher has a responsibility to impart knowledge, encourage participation, promote new skills, and cater for student needs, however, the student must also take responsibility for their own learning, and it is the teacher who must foster the motivation and ability within the student to do so. With our world constantly changing and evolving, cultivating the ability to think critically and independently is essential and I believe the use of different technologies in the classroom, along with other resources, assists in imparting and developing such essential skills and knowledge.
Organisation and structure within a classroom are fundamental in providing and maintaining a supportive, safe and productive environment, however the ability to be flexible and take into account the individual needs and motivations of students is essential in fostering an engaging, stimulating, and motivating classroom that caters for the diverse needs of all students. It is my belief that it is the role of the teacher to provide a variety of ways for students to access, interpret, analyse, and utilise the information and skills we teach them and I am constantly striving to gather new techniques, find or create resources, and engage in professional discussion and development with colleagues to ensure I am able to provide the best possible learning experience for each student. Knowledge, understanding, and connection with students are paramount in the ability to cater for the variety of needs and learning styles within a classroom and I consider the development of rapport with my students as the foundation of my teaching style. Keeping abreast of popular culture and new technologies is a way for me to create relationships with my students and show an interest in things that are relevant or important to them. I also have many work, study, travel, and life experiences that I draw on as a teacher to assist the way I interact with, relate to, and understand my students.
It is this philosophy of engaging and building relationships that drives my enthusiasm for using and promoting technological resources. In our digital age, using technology in the classroom not only stimulates students, it prepares them for a future where technology plays a key role. So many companies are producing innovative, practical, and engaging digital resources for teachers that we'd be foolish not to take advantage of them. Technology allows me to more easily and flexibly differentiate in my classroom in a way that conventional methods do not, and while I'm overtly teaching them the skills and knowledge of my subject area and catering to their individual needs, students are also learning a great deal of practical skills that will serve them well in the future. Of course, balance is key when it comes to technology in the classroom and I'm always conscious of not just using technology for the bells and whistles effect, but rather ensuring that it is useful and enhances student learning and engagement. Sharing and discussing these valuable resources, techniques, and experiences with colleagues and peers is also an essential component of being an effective and passionate teacher.
Nothing is more rewarding than that lightbulb moment when a child understands something, the joy you see on their faces when they master something they've been struggling with, seeing the improvement in behaviour and motivation from one term to the next, and the gratitude of parents who have watched their child blossom under your care. These are the things that motivate and encourage me as a teacher. These are the things that I strive for in my classroom and, because I'm constantly learning and developing who I am as an educator, the digital technologies featured on this site play a large role in helping me to create an engaging, diverse classroom, shaping my teaching style, and stimulating my passion for teaching.


text:
ReVolt Technology Moves European Headquarters from Switzerland to Dortmund, Germany
ReVolt Technology, a technology company which is developing innovative, rechargeable zinc-air batteries, today announced that it has re-established its European headquarters from Switzerland to Dortmund, Germany at the Zentrum für Produktionstechnik Dortmund (ZfP), Carlo-Schmid-Allee 3.
"ReVolt Technology concluded its decision to relocate to Dortmund and North Rhine-Westphalia, after an extensive review of strategic localization factors including: commitment to public policy supporting the adoption of green technologies, commitment to the environment in everyday life by the society, skilled workforce, location of strategic partners and investors and universities, a diverse and strong local and national economy with solid growth prospects combined with an attractive array and strong commitment of economic development assistance through each stage of the company's growth," stated James P. McDougall, CEO and Managing Director of ReVolt Technology GmbH.
"The relocation of Revolt Technology's European Headquarters confirms the attractiveness of North Rhine-Westphalia for Electromobility research and applications," said NRW's Minister of Economic Affairs Harry K. Voigtsberger." Energy storage solutions like the ones being developed by Revolt Technology GmbH are making an important contribution to our goal of putting at least 250,000 electric vehicles on the road by 2020 in North Rhine-Westphalia. Electric vehicles will help us to solve problems with air pollution and noise pollution."
ReVolt Technology was selected by North Rhine-Westphalia for a grant focused on developing energy storage solutions for electric mobility. The company was recently granted approval from the state of North Rhine-Westphalia to commence the project. Revolt was selected by a panel of experts from academia and industry panel of judges before a total of 54 project proposals. The final 22 projects selected will be funded with 46.5 million Euros from EU and state funds.
In addition, ReVolt Technology GmbH will benefit from access to modern building and equipment infrastructure at the ZfP, providing optimal conditions for the continuation of their research and development activities. The ZfP was commissioned by the city of Dortmund. ReVolt Technology GmbH will be supported by ZfP during its build out and build up through introductions to partners for development and financing.
"Dortmund is a Hightech hub that offers a compelling environment for the development of new technologies," said Udo Mager, Managing Director of the Economic Development Agency Dortmund. "The Phoenix West location decision is another success and further step forward in securing new technology developments for the former mining area.
"ReVolt's research and development focus is a perfect fit with the technology portfolio of the ZfP in Dortmund," said Guido Baranowski, Managing Director of the Dortmund Technology Center. "We are delighted that we were able to attract them to the High-Tech Hub of Dortmund with our ready-made technical infrastructure and business support."
"We are honored to become a member of the local business community of North Rhine-Westphalia and excited to bring our program focused on development of the next generation of energy storage for low-cost, environmentally-friendly, high-energy applications for consumer and industrial applications," stated CEO and Managing Director, James P McDougall. "The robust ecosystem of North Rhine-Westphalia combined with its long-term commitment to supporting the development of transformational energy storage solutions is indeed very impressive and compelling for us to relocate and make a long-term commitment to the region."
The move to Germany will place the heart of ReVolt's European headquarters much closer to many of its strategic partners to better facilitate cooperation and coordination.
"With this step the state of North Rhine-Westphalia supports the development of an innovative and efficient storage technology in its own home province, which has also convinced RWE Innogy. This is because energy supply in the future will depend on the various options of storing energy. For this reason, we had acquired a minority stake in Revolt as part of our venture capital activities back in January 2009 and have been supporting the company in their further development ever since. We are happy that we are now moving closer together and that North Rhine-Westphalia receives another building block to grow into a technology stronghold."
Initially developed in Norway, ReVolt's patented zinc-air technology is based on research conducted at one of Scandinavia's top scientific institutes. ReVolt's zinc-air, rechargeable battery technology provides a high energy solution for consumer and industrial market applications that is environmentally safe, reusable and recyclable. Revolt's zinc-air technology offers up to three times the energy density of lithium-ion in an inherently safe and cost-effective package representing a major leap forward and compelling value proposition for energy storage applications.
ReVolt Technology is a technology company, which is developing a rechargeable zinc-air battery. ReVolt's batteries may enable more high-power consumer devices, electric vehicles and renewable energy storage. The technology is a result of six years of intense research and development at SINTEF (the largest independent research institute in Scandinavia). Research on material combinations has solved issues historically related to the metal-air technology; power, lifetime and rechargeability.
www.revolttechnology.com
aerogeneradoreseólicaeólicoLatinoaméricaPerúSalka Perú
Previous PostPortland General Electric Reports Third Quarter 2010 Financial ResultsNext PostThe World?s Largest Concentrating Solar Power Project Finds a Home in California

redpajama_set_name: RedPajamaCommonCrawl


text:
Q: the_posts_navigation is not working Unfortunately, the_posts_navigation() function is not working for me. When I click on 'Older Posts' it's showing '404 page  I don't know why my code is not working.
Here is the code -

    <?php while ( have_posts() ) : the_post(); ?>

        <a class="thmb" href="<?php echo get_post_meta ($post->ID, 'link_name', true); ?>" target="_blank">
        <?php
        the_post_thumbnail( 'website-image-size', array( 'class' => 'img-responsive' ) );
    ?>

<?php the_title(); ?>

        by <?php echo get_post_meta ($post->ID, 'author_name', true); ?>

<?php endwhile; ?>

<?php the_posts_navigation(array(
    'prev_text' => 'Older Posts',
    'next_text' => 'Newer Posts',
    'screen_reader_text' => ' ',
));
?>

It's showing the same error even I use the previous_posts_link() function.

A: This function uses get_the_posts_pagination() which uses the GLOBAL $wp_query to setup the paginate_links() function, so I believe that doesn't work for get_posts.
Try use the function paginate_links() by itself or the function posts_nav_link()

A: Place the_posts_navigationinside while loop hopefully it works!!

redpajama_set_name: RedPajamaStackExchange


While we are very eager and excited to get the 2018 WaveSpec KCRSCCA RallyCross Championship season started, we also want to make sure that our events are fun and safe for our participants and spectators, and that we are good stewards of the venues at which we compete. The heavy rains on Tuesday coupled with the rains over the next three days, including Saturday morning, have caused the 2018 KCRSCCA RallyCross Advisory Committee (RAC) to postpone this weekend's RallyCross Event #1 at the Thunder Valley Sand Drags.
The Thunder Valley Sand Drags has been a great partner for our growing program over the past four years, but one thing we've learned about the site is that it is very susceptible to excessively muddy conditions as those who were at the 2015 MiDiv RallyCross Challenge may remember. Rather than attempting a shortened course on the firmer areas of the site or risking getting cars and people stuck in the deep clay mud of the drag strip run-out, the RAC is exploring various possible future dates to reschedule the event, including the possibility of having a double-header event in the Fall. We will make sure to keep everyone up to date as decisions for the restructuring of our 2018 event schedule are made.
In the meantime, registration for the 2018 WaveSpec KCRSCCA RallyCross Event #2 will open on Saturday! (maybe we relabel this Event #1b?) Those who preregistered and prepaid for this weekend's Event #1 will receive a credit on MotorsporReg.com that can be applied to a future KCRSCCA RallyCross event, including Event #2 on March 24th. Again, we apologize for having to cancel this first event. We're certainly not against getting dirty, but no one likes getting stuck fender deep in the mud.
Tagged Canceled, Rainout, Rallycross, Thunder Valley Sand Drags. Bookmark the permalink.


Lot 4 Section 1 Fox Hill is a $38,000 home on a 3.88 acre lot located in Stedman, NC.
-Fox Hills-Beautiful 3.88ac cleared lot,perfectfor building a custom home.Subdivision price range $260-380,000, min. 1800 sq ft. HOA:$121/yr.Septic tank,well,horses allowed.Water frontage.
I am interested in Lot 4 Section 1 Fox Hill, Stedman, NC 28391.


I've been blessed by the god of travel, and during those journeys I have stepped on American soil three times.
My first visit was at the tail end of last century, where entering LA airport was like walking onto the Holodeck from Star Trek. You see, a yellow cab or a black and white police car, or even the design of a US security uniform, are only ever seen as elements of Hollywood art design. Your mind associates them with works of fiction.
So it was hard to take this airport as real. I expected the guards to have fake guns and for the windows to suddenly smash as a stuntman dived through. It was one big movie set on display for visitors to America.
During that trip I decided to cook for friends we were staying with in Olympia. Going to the supermarket was a case of 'you say tomay-to, I say toma-toe'. Fortunately, I had a very helpful staff member translate my shopping list.
Slowly we worked out that my chilli con carne recipe was simply called a bowl of chilli by the locals. A capsicum was a bell pepper. But then that chilli word became a problem again. Explaining minced chilli became fruitless to my confused shop assistant. Finally I found what I was after. A tube of minced peppers. Ironically that tube came from down-under and was labelled minced chilli.
It's small stuff like this that made me want to write a tale of a relationship between an American and an Australian. To explore those odd mismatches you stumble on when you bring characters from those two cultures together.
Like me, my Australian character, Nathan, believes an entrée is an appetizer. Like me, Nate can't think in Fahrenheit. He's also someone who jumps into the passenger seat in a taxi, so he can enjoy conversation with the driver face to face. And he's someone who has to remember that the price of an item in a shop is not the final price of that item at the cash register.
We learn to change into 'America mode' when visiting the land where zed is pronounced zee and chips are French fries. Where when you shop for fabric to bring back home, you recall that a yard is the same length as a long playing record.
During one of our travels we took the plunge and hired a car, getting used to sitting in the wrong seat to drive on the opposite side of the road. All was okay when we were following traffic. It was when we were at the front of the pack about to turn into a street, that we had to fight our natural inclination to move to the left. Often it was a case of checking which way the parked cars were facing to guide us to the correct side of the road. Our fear of a head-on collision was averted many times. And, naturally, we chose the kilometre option on the GPS.
On my first visit I needed to use directory assistance from a payphone, and the operator asked me to place a dime in the slot. When I asked which currency was a dime, he told me which president was on the coin instead of simply telling me it was ten cents, making me none-the-wiser.
It's these weird twists that you remember about travelling. About your first impression of New York where you feel you've entered a large scale version of Sesame Street. About tasting biscuits and gravy and knowing you never want to eat them again. About remembering not to visit the Empire State Building on a foggy day. About the weather turning cold at Christmas.
Of course there are some major differences when taking in the American way of life, but these don't touch a reader when throwing an Australian into the New York landscape. My character Nate is negotiating all these minor changes to his life on top of a relationship. And fortunately I have those experiences to help guide him.
Coming to an Amazon near you shortly!
All those cultural differencies between people are always fascinating. Thank you Anna for this guest post and thank you Kevin Klehr for sharing. You're a new author to me and will defnitely check your books!
It's fascinating because in so many ways we're alike, so when the differences become apparent, they can be quite jarring.
Hope you have a wonderful time in NY. It's a great city.


100m2 of open plan space filled with lights and plants. Roof terrace (40m2) with a fire pit.
A real private cinema with one of the best AV systems. 9 reclinable luxury leather seats and 3 additional bean bags.


text:
Trump Just Said There's Actually No Need For A Full Border Wall
The president also told reporters the wall should be transparent so people in the US can avoid large bags of drugs being thrown over the barrier.
by Adolfo Flores
Kevin Lamarque / Reuters
President Trump significantly scaled back one of his key campaign pledges while en route to France, telling reporters there was no need to build a wall along the full length of the US–Mexico border.
"It's a 2,000-mile border, but you don't need 2,000 miles of wall because you have a lot of natural barriers. You have mountains. You have some rivers that are violent and vicious," Trump said while on Air Force One Wednesday. "You have some areas that are so far away that you don't really have people crossing. So you don't need that."
Trump estimated the US would only need to build a wall on 700 to 900 miles of the border, and said the structure would need to be transparent so that people could avoid large sacks of drugs being thrown over from the other side.
Guillermo Arias / AFP / Getty Images
A view from the Mexican side of the US–Mexico border in Playas de Tijuana, Mexico, in 2017.
"One of the things with the wall is, you need transparency. You have to be able to see through it," Trump said. "As horrible as it sounds, when they throw the large sacks of drugs over, and if you have people on the other side of the wall, you don't see them — they hit you on the head with 60 pounds of stuff? It's over. As crazy as that sounds, you need transparency through that wall."
Trump has backtracked on his initial campaign pledge to build a large, impenetrable wall — which he once described as being made out of "precast [concrete] plank" — before.
After winning the election, he told 60 Minutes that he'd be open to fencing in some stretches, and he has referred to treating natural landmarks such as rivers and mountains as barriers before.
Based on his comments while en route to Paris, Trump now says the US will only build a wall on less than half the 2,000-mile US–Mexico border, but much of that is already fortified with a physical barrier. Just 350 miles lack a vehicle barrier or fence, but they are monitored with remote technology, lights, and patrols.
Trump appears to be operating in a post-campaign reality of political and financial roadblocks.
A key tenet of his border promise is that Mexico would pay for it, something Mexico has repeatedly rejected. That would leave the US with a huge bill for the brick-and-mortar wall Trump pledged, with costs potentially ballooning up to $40 billion, according to MIT Technology Review.
Even for the fence option, PolitiFact reported that it could cost between $5.1 billion and $25 billion, plus additional money to maintain it.
In June, Trump said his administration wanted to build a wall covered with solar panels "so it creates energy and pays for itself." But experts say it wouldn't be easy, and makes little economic sense.
But Trump doubled down on the idea Wednesday, saying "there's no better place for solar than the Mexico border."
"Plus, we have some wall that's already up that we're already fixing," he said, referring to reinforcing existing barriers. "You know, we've already started the wall because we're fixing large portions of wall right now. So in a true sense, we've already started the wall."
Here's Why Trump's "Great Wall" On The US–Mexico Border Is Basically A Pipe Dream
Trump Wants To Build A Solar Wall With Mexico. Solar Experts Say That's "Stupid And Pointless."
Adolfo Flores is a reporter for BuzzFeed News and is based in McAllen, Texas..
Contact Adolfo Flores at adolfo.flores@buzzfeed.com.


Many people dream of the ability to work from anywhere, and imagine themselves telecommuting from a condo in Thailand one month and cafe hopping in London the next.
But for most telecommuters, work happens in less exotic places. Many have a home office, which is convenient, but can be lonely and full of too many distractions like children, pets or TV. Some take their laptop to coffee shops, but there's no guarantee that an empty table will be available. Plus, ordering the same drink from the same shop every day can start to feel like a rut.
Luckily, there are other places to cowork, and some include perks like drinks, snacks, high-speed WiFi, storage, meeting spaces and the chance to collaborate with others, all for less than a typical office rental.
Some restaurant owners are welcoming coworkers with special accommodations, such as Boccato, a coffee shop in Arlington, Virginia. The shop has a reserved area called CoworkCafe, which is open on weekdays from 8:30 AM to 6 PM. During this time, members work comfortably from desks, tables or couches, enjoy a steady stream of coffee, sandwiches and gelato, and draw inspiration from others.
The cafe also offers high-speed WiFi, office supplies, mailboxes, conference space and phone booths for private conversations. A monthly membership at CoworkCafe costs $150, which includes a $50 food credit (many coworking spaces in Arlington cost at least twice as much).
CoworkCafe prides itself on being affordable, but also for providing an environment that is conducive to productivity. "Having a place that's relaxed and comfortable is very good for creative type work," cofounder David James says. "There's a certain feeling that you get in a place like this you can't get in an office-type building. They really love the feeling of the space; they don't want to be in a traditional office setting."
For those who live in New York, the company Spacious has turned some high-end restaurants into coworking spaces during the day. Membership is $95 a month and includes access to all Spacious locations, WiFi, coffee, tea and meeting space. The company currently has plans to expand to Los Angeles, San Francisco and London.
With free WiFi, access to beverages and comfortable tables and couches, hotel lobbies can be an excellent coworking environment. Some hotels even welcome coworkers, since they make the lobby appear lively and popular.
The most famous hotel lobby for coworking is that of the Ace Hotel, which is a haven for tech entrepreneurs, designers, writers and other professionals, due to its creative and social atmosphere. People work side-by-side at long tables equipped with outlets and desk lamps, bouncing ideas off of each other. Others keep their eyes on their own screen and use earbuds to drown out the chatter.
Not all hotel lobbies have such a welcoming, collaborative atmosphere, so you may need to try out a few before settling on your new coworking spot. WiFi can also be hit and miss, so you'll want to test it out or visit Hotel WiFi Test to find the hotels with the fastest WiFi near you.
Designated coworking spaces are popping up all over the world, and give coworkers the opportunity to connect with designers, engineers, writers and other professionals, with amenities once only thought possible at Google.
Enjoy events like communal breakfasts, lunch-and-learns and happy hours at The Coop in Chicago, or relax in the Japanese Zen Garden at Coco in Minneapolis. Women many prefer HeraHub in San Diego and D.C., a spa-inspired coworking space for female professionals only, where aromatic candles, soft music and chair massages provide the ultimate in relaxed work environments.
For those who don't want to shell out $50-$350 a month, some coworking spaces like Gangplank in Arizona are free, as long as you participate in projects that help out the community as well as fellow members. Seats2Meet offers free coworking spaces around the world, with the promise that you'll use your expertise to help others.
If you crave variety and don't want to commit to a single space, try CoPass, where you can work in a different workspace everyday with one monthly membership.
With plenty of seating, plentiful food and beverage options and free WiFi, the mall is emerging as a new coworking hotspot. Since most of the foot traffic occurs on the evenings and weekends, you may be pleasantly surprised how quiet it can be during the workweek.
Westfield Corp recently capitalized on this trend by building a designated coworking, event and technology demo space called Bespoke at their San Francisco mall. The coworking area includes desks, open workspace and private offices, as well as a rock climbing wall, a bocce ball court and a library with sleep nooks. Membership fees start at $395 a month for a shared desk.
Bespoke is the first coworking space in a U.S. shopping mall, but similar spaces exist in other parts of the world, such as Multispace at the Astana Mall in Kazakhstan and centers operated by Space&Co in Australia.
For those looking for more adventure in their work environment, there are many vacations and retreats that cater to coworkers. With Remote Year, 75 professionals travel the world together while working remotely. The group visits one new city a month for a year, including Prague, London, Kuala Lumpur and Buenos Aires. If you're looking for a smaller commitment, Hacker Paradise offers trips that last 2 to 12 weeks at a time.
Whether you're looking for a free and comfortable place to work, top-of-the-line amenities or the ability to brainstorm with like-minded individuals, there's a coworking place to fit your needs. And if you choose to jet-set around the world for a year, you may be happy to return to a familiar workplace: home.


Gadgets are not the perfectionists' toy. Even after years of thorough research and discussions on design, something may go wrong at any time leaving some unforeseen flaws with gadgets before they reach actual users.
This is something that lets down both the OEMs and the customers after the launch of a device.
However, there are not many good boys around here, with a history clean of rolling out hundred percent perfect devices. HTC is notorious with design flaws with its earlier devices like Nexus One when multi-touch issues were reported and again when the EVO 4G annoyed many users with improperly mounted screens that caused cracks on the body of the device.
Now the Taiwanese company has to play efficient PR and mitigation measures since their new flagship device HTC One X is said to be suffering from similar design flaws on its touch screens.
Though the existence of the flaw is yet to confirmed, certain demo models received by the press has shown unusual pattern of selection even when the user holds the phone tightly in their hand.
Certain sources have also reported that pushing on the edges of the curved display causes it to flex and form patches of discolored pixels with the finger movements as you can watch in the video.
This makes accidental selection more possible if the user holding the phone exerts some extra pressure unintentionally on certain circumstances.
However, HTC officials made it clear that they are unaware of such an issue with units ready for shipping.
No units shipped to the users will have such flaw, they said. Presently it is assumed that the flaw was only found on earlier demo pieces.
If you came across anything similar, let us know.


text:
<?php defined('BASEPATH') OR exit('No direct script access allowed');

class Editors extends MX_Controller
{
	function __construct()
	{
		parent::__construct();
		$this->load->model('users/users_model');
		$this->load->model('editors_model');
		$this->load->helper('date');
		$this->load->library('auth/ion_auth');
		if(!$this->ion_auth->logged_in())
		{
			redirect('/auth/login/','refresh');
		}
		if(!$this->ion_auth->in_group('editor'))
		{
			redirect('/users', 'refresh');
		}
	}

	function index()
	{
		//TODO : make some home page
		$this->unapproved_papers();
	}

	function render_page($page, $data)
	{
		$id = $this->ion_auth->user()->row()->id;
		$data['user'] = $this->users_model->get_userdata($id);
		$data['unapproved_number'] = count($this->editors_model->get_unapproved_papers());
		$this->load->view('editors_dash', $data);
		$this->load->view($page, $data);
		$this->load->view('footer', $data);
	}

	function unapproved_papers()
	{
		$data['papers'] = $this->editors_model->get_unapproved_papers();
		$this->render_page('unapproved_papers', $data);
	}

	function approve_paper()
	{
		if($this->input->post('action') && $this->input->post('id'))
		{
			$this->editors_model->approve_paper($this->input->post('action'), $this->input->post('id'));
		}
	}
}
?>

redpajama_set_name: RedPajamaGithub


2 in 1 car seat stroller a long term popular brand focus on manufacturing and selling baby strollers baby car seats infant car seats baby carriers baby baby car seat stroller 2 in 1.
ikea 18x24 frame wonderful poster frames picture frame poster frames ikea canada 18x24 frame.
bottle of hennessy cost leave a review hennessy bottle prices abc store.
shade tech instant canopy shade tech ii instant canopy square feet of shade only open box for sale in shade tech instant canopy 12x12.
rustic string lighting rustic outdoor lighting patio traditional with string lights pizza ovens furniture rustic outdoor string lighting.
bike trainer stand road bike wheel stand station professional bike trainer booster device riding station front wheel bicycle indoor bicycle trainer stand walmart.
rechargeable solar batteries rechargeable ah china solar battery for solar energy home rechargeable solar batteries for sale.
jar opener 5 in 1 bottle and jar opener jar opener rubber disk.
boost mobile sim kit and slot for inside mobile phone boost mobile starter kit prepaid sim card unlimited 64k.


Children at primary schools in the Buckie area are to help decide the name of Moray Council's new £2.4million dredger.
A competition is being run over the next few weeks when pupils will be asked to suggest a suitable name for the vessel, which is currently under construction at the Macduff Shipyard site at Buckie harbour.
A shortlist will then go before councillors for a final decision.
The dredger is due for completion next spring and will be based at Buckie, although she will also service the harbours at Burghead, Hopeman, Findochty, Portknockie and Cullen as well as being available for hire to coastal councils elsewhere in Scotland.
The vessel – which will be the only council-owned dredger in Scotland - is being built as a replacement for the Shearwater, which Moray Council disposed of two years ago after many years of service.
The naming competition is open to pupils at Cluny, Millbank, Portessie, St Peter's, Portgordon, Findochy, Portknockie and Cullen primary schools.
The pupil whose entry is selected will be given a tour of the Macduff Shipyard site to see the dredger under construction and to see the nameplate attached to the bow.


Obituary for Julius Neely, Jr.
Mr. Julius Neely, Jr., 86, of Elizabethtown, KY died on August 7, 2018 at his residence.
Mr. Neely was a native of Hartsville, TN and the son of the late Julius and Susie Neely. He was a U. S. Army veteran.
In addition to his parents, he was preceded in death by his wife, Josephine Neely; his siblings, Willie Lee Neely, J. C. Neely, Hortense Holland, Rosa Lee Wright, Elizabeth Alexander and Maliza Sullivan.
Survivors include one son, Julius W. Neely of Elizabethtown, KY; one daughter, Queen Victoria Barber of Fairview Heights, IL; three grandchildren, Ryan Neely (Julia), Brandon Barber and Erin Neely; a host of nieces, nephews, cousins and other relatives.
Funeral service will be 2:00 pm on Thursday, August 16, 2018 at Strawther & White Funeral Home in Gallatin, TN with Rev. John Alexander officiating. Burial with military honors will be 11:00 am on Friday, August 17, 2018 at West Tennessee State Veterans Cemetery in Memphis, TN.
Visitation will be from 1:00 to 2:00 pm on Thursday, August 16, 2018 at Strawther & White Funeral Home.


HealthDay News — Attention to preoperative stress in adolescents undergoing scoliosis surgery may reduce levels of postoperative pain as well as anxiety and social and attention problems in the recovery period, according to a study published online February 22 in the Journal of Clinical Nursing.
Anna-Clara Rullander, RN, PhD, from Umeå University in Sweden, and colleagues assessed 37 adolescents' experiences before scoliosis surgery and again at six to eight months after surgery.
The researchers found that rates of anxiety/depression and internalizing behavior were significantly higher before surgery than at six months after. There was a significant correlation between preoperative anger, social problems, and attention problems and postoperative pain on day three. There was a similar correlation between postoperative pain and anxiety, social problems, and attention problems at follow-up.
"The results of this study indicate a need for interventions to reduce perioperative stress and postoperative pain to improve the quality of nursing care," the authors write.


Learn the nation's fastest growing sport with help from the US Sports Institute! Our lacrosse camps and classes provide the perfect opportunity for players of all ability levels to learn and enhance their skills. These programs will ensure participants can learn the game in a safe and fun environment.
At a USSI lacrosse program, participants will learn the fundamental skills of lacrosse including scooping, cradling, passing, and scoring.
Not sure which is the correct lacrosse program for your child?
Players will learn basic skills through fun games and activities.
A skill-based program with an introduction to scrimmaging.
A non-contact, fun way to learn skills and scrimmage.
For the more serious player looking for a competitive program.


text:
import React from 'react';

const Backspace = () => <noscript />;

Backspace.updateCursor = (cursor, { speed, count, delay }) => {
  return {
    ...cursor,
    numToErase: count,
    preEraseLineNum: cursor.lineNum,
    speed: speed > 0 ? speed : cursor.speed,
    delay: delay > 0 ? cursor.delay + delay : cursor.delay,
  };
};

Backspace.getName = () => 'Backspace';

export default Backspace;


Materials Used: 150-200 yards of yarn in your main color and 100-150 yards of yarn in your contrast color.
Pattern Includes: Written direction and photos. Pattern also includes 3 samples knit in various gauge yarns and a fun story about Basil.


text:
Ori D. Rotstein
Oxidative stress generated by hemorrhagic shock recruits Toll-like receptor 4 to the plasma membrane in macrophages
Kinga A. Powers, Katalin Szászi, Rachel G. Khadaroo, Patrick S. Tawadros, John C. Marshall, András Kapus, Ori D. Rotstein
J Exp Med (2006) 203 (8): 1951–1961.
DOI: https://doi.org/10.1084/jem.20060943
Oxidative stress generated by ischemia/reperfusion is known to prime inflammatory cells for increased responsiveness to subsequent stimuli, such as lipopolysaccharide (LPS). The mechanism(s) underlying this effect remains poorly elucidated. These studies show that alveolar macrophages recovered from rodents subjected to hemorrhagic shock/resuscitation expressed increased surface levels of Toll-like receptor 4 (TLR4), an effect inhibited by adding the antioxidant N-acetylcysteine to the resuscitation fluid. Consistent with a role for oxidative stress in this effect, in vitro H2O2 treatment of RAW 264.7 macrophages similarly caused an increase in surface TLR4. The H2O2-induced increase in surface TLR4 was prevented by depleting intracellular calcium or disrupting the cytoskeleton, suggesting the involvement of receptor exocytosis. Further, fluorescent resonance energy transfer between TLR4 and the raft marker GM1 as well as biochemical analysis of the raft components demonstrated that oxidative stress redistributes TLR4 to lipid rafts in the plasma membrane. Preventing the oxidant-induced movement of TLR4 to lipid rafts using methyl-β-cyclodextrin precluded the increased responsiveness of cells to LPS after H2O2 treatment. Collectively, these studies suggest a novel mechanism whereby oxidative stress might prime the responsiveness of cells of the innate immune system.
Host Resistance to Intracellular Infection: Mutation of Natural Resistance-associated Macrophage Protein 1 (Nramp1) Impairs Phagosomal Acidification
David J. Hackam, Ori D. Rotstein, Wei-jian Zhang, Samantha Gruenheid, Philippe Gros, Sergio Grinstein
J Exp Med (1998) 188 (2): 351–364.
DOI: https://doi.org/10.1084/jem.188.2.351
The mechanisms underlying the survival of intracellular parasites such as mycobacteria in host macrophages remain poorly understood. In mice, mutations at the Nramp1 gene (for natural resistance-associated macrophage protein), cause susceptibility to mycobacterial infections. Nramp1 encodes an integral membrane protein that is recruited to the phagosome membrane in infected macrophages. In this study, we used microfluorescence ratio imaging of macrophages from wild-type and Nramp1 mutant mice to analyze the effect of loss of Nramp1 function on the properties of phagosomes containing inert particles or live mycobacteria. The pH of phagosomes containing live Mycobacterium bovis was significantly more acidic in Nramp1- expressing macrophages than in mutant cells (pH 5.5 ± 0.06 versus pH 6.6 ± 0.05, respectively; P <0.005). The enhanced acidification could not be accounted for by differences in proton consumption during dismutation of superoxide, phagosomal buffering power, counterion conductance, or in the rate of proton "leak", as these were found to be comparable in wild-type and Nramp1-deficient macrophages. Rather, after ingestion of live mycobacteria, Nramp1-expressing cells exhibited increased concanamycin-sensitive H+ pumping across the phagosomal membrane. This was associated with an enhanced ability of phagosomes to fuse with vacuolar-type ATPase–containing late endosomes and/or lysosomes. This effect was restricted to live M. bovis and was not seen in phagosomes containing dead M. bovis or latex beads. These data support the notion that Nramp1 affects intracellular mycobacterial replication by modulating phagosomal pH, suggesting that Nramp1 plays a central role in this process.
Rho is Required for the Initiation of Calcium Signaling and Phagocytosis by Fcγ Receptors in Macrophages
David J. Hackam, Ori D. Rotstein, Alan Schreiber, Wei-jian Zhang, Sergio Grinstein
Phagocytosis of bacteria by macrophages and neutrophils is an essential component of host defense against infection. The mechanism whereby the interaction of opsonized particles with Fcγ receptors triggers the engulfment of opsonized particles remains incompletely understood, although activation of tyrosine kinases has been recognized as an early step. Recent studies in other systems have demonstrated that tyrosine kinases can in turn signal the activation of small GTPases of the ras superfamily. We therefore investigated the possible role of Rho in Fc receptor–mediated phagocytosis. To this end we microinjected J774 macrophages with C3 exotoxin from Clostridium botulinum, which ADP-ribosylates and inactivates Rho. C3 exotoxin induced the retraction of filopodia, the disappearance of focal complexes, and a global decrease in the F-actin content of J774 cells. In addition, these cells exhibited increased spreading and the formation of vacuolar structures. Importantly, inactivation of Rho resulted in the complete abrogation of phagocytosis. Inhibition of Fcγ receptor–mediated phagocytosis by C3 exotoxin was confirmed in COS cells, which become phagocytic upon transfection of the FcγRIIA receptor. Rho was found to be essential for the accumulation of phosphotyrosine and of F-actin around phagocytic cups and for Fcγ receptor–mediated Ca2+ signaling. The clustering of receptors in response to opsonin, an essential step in Fcγ-induced signaling, was the earliest event shown to be inhibited by C3 exotoxin. The effect of the toxin was specific, since clustering and internalization of transferrin receptors were unaffected by microinjection of C3. These data identify a role for small GTPases in Fcγ receptor–mediated phagocytosis by leukocytes.


text:
angular.module('wami.recorder', [])

    .constant('wamiSettings', {

        // Audio options
        container: "wav",
        encoding : "pcm",
        sampleRate: 44100,
        numChannels : 2,
        interleaved: true,

        // SWF options
        swfUrl: 'Wami.swf',
        wmode: "transparent",
        swfWidth: 214,
        swfHeight: 137,
        noSecurityCheck: false,

        // Server options
        serverUrl: null,
        idPrefix: '',

        // Callbacks
        onRecorderLoaded: function () {},
        onRecorderReady: function () {},

        onRecorderSecurityGranted: function () {},
        onRecorderSecurityFailed: function () {},

        onRecordingStart: function () {},
        onRecordingFinish: function () {},
        onRecordingFail: function () {},

        onPlaybackStart: function () {},
        onPlaybackFinish: function () {},
        onPlaybackFail: function () {},

    })

    .factory('WamiRecorder', ['$log', 'wamiSettings',
        function ($log, wamiSettings) {

            function Recorder (options) {
                angular.extend(wamiSettings, options);
                this._options = wamiSettings;

                if (!this._options.serverUrl) {
                    $log.error('Wami: No serverUrl was specified. Please make sure that WamiRecord.init() is called with a url that points to a valid upload server.');
                    return;
                }

                if (!swfobject) {
                    $log.error('SWFObject was not found. Please make sure that the swfobject.js dependency has been included in the page.');
                    return;
                }

            }

            Recorder.prototype = {

                embed: function (containerId, loadedCallback) {
                    var self = this;
                    loadedCallback = loadedCallback ||
                        Wami.nameCallback(function () {
                            console.log('calling delegate');
                            self.delegateApi();
                        });
                    var flashVars = {
                        visible: false,
                        loadedCallback: loadedCallback
                    }

                    var params = {
                        allowScriptAccess: "always",
                        wmode: this._options.wmode
                    };

                    var container = document.getElementById(containerId);
                    container.style.position = 'absolute';
                    this._container = container;

                    var version = '10.0.0';
                    recorderDiv = document.createElement('div');
                    var recorderId = Wami.createID();
                    recorderDiv.setAttribute('id', recorderId);
                    recorderDiv.innerHTML = "WAMI requires Flash "
                                    + version
                                    + " or greater
https://get.adobe.com/flashplayer/";

                    container.appendChild(recorderDiv);

                    // This is the minimum size due to the microphone security panel
                    swfobject.embedSWF(
                        this._options.swfUrl,
                        recorderId,
                        this._options.swfWidth,
                        this._options.swfHeight,
                        version,
                        null,
                        flashVars,
                        params,
                        {},
                        function () {
                            self.recorder = document.getElementById(recorderId);
                        });

                    // Without this line, Firefox has a dotted outline of the flash
                    swfobject.createCSS("#" + recorderId, "outline:none");

                },

                embedSwf: function () {

                },

                getSettings: function () {
                    var settings = this.recorder.getSettings();
                    settings.microphone.remembered = Wami._remembered;
                    return settings;
                },

                delegateApi: function () {
                    var self = this;
                    function delegate(name) {
                        self[name] = function() {
                            return self.recorder[name].apply(self.recorder, arguments);
                        }
                    }

                    delegate('startPlaying');
                    delegate('stopPlaying');
                    delegate('startRecording');
                    delegate('stopRecording');
                    delegate('startListening');
                    delegate('stopListening');
                    delegate('getRecordingLevel');
                    delegate('getPlayingLevel');
                    delegate('setSettings');

                    if (!this._options.noSecurityCheck) {
                        this.checkSecurity();
                    }

                },

                checkSecurity: function () {
                    var settings = this.recorder.getSettings();
                    console.log(settings);
                    if (settings.microphone.granted) {
                        console.log('granted');
                        this._options.onRecorderReady();
                    } else {

                        // Show any Flash settings panel you want:
                        // http://help.adobe.com/en_US/FlashPlatform/reference/actionscript/3/flash/system/SecurityPanel.html
                        this.showSecurity("audio", "Wami.show",
                            Wami.nameCallback(this.checkSecurity),
                            Wami.nameCallback(function (error) { console.error(error)}));
                    }
                },

                showSecurity: function(startfn, finishedfn, failfn) {
                    var self = this;
                    console.log(this._container);
                    var augmentedfn = Wami.nameCallback(function() {
                        self.checkRemembered(finishedfn);
                        self._container.style.cssText = "position: absolute;";
                    });

                    this._container.style.cssText = "position: absolute; z-index: 99999";

                    this.recorder.showSecurity(panel, startfn, augmentedfn, failfn);
                },

                checkRemembered: function(finishedfn) {
                    var id = Wami.createID();
                    var div = document.createElement('div');
                    div.style.top = '-999px';
                    div.style.left = '-999px';
                    div.setAttribute('id', id);
                    var body = document.getElementsByTagName('body').item(0);
                    body.appendChild(div);

                    var fn = Wami.nameCallback(function() {
                        var swf = document.getElementById(id);
                        Wami._remembered = swf.getSettings().microphone.granted;
                        Wami.swfobject.removeSWF(id);
                        eval(finishedfn + "()");
                    });

                    this.embed(id, fn);
                }

            }

            return {

                getRecorder: function (options) {
                    return new Recorder(options);
                },

            };
    }])

    .directive('wamiRecorder', ['WamiRecorder', function (WamiRecorder) {
        return {
            restrict: 'A',
            scope: {
                wamiRecorder: '='
            },
            link: function ($scope, $element, $attrs) {
                $scope.$watch('wamiRecorder', function (options) {
                    if (options) {

                        recorder = WamiRecorder.getRecorder(options);
                        recorder.embed($attrs.id);

                        $scope.startRecording = function () {
                            recorder.startRecording();
                        }

                        // angular.extend(wamiSettings, options);

                        // Wami.setup({
                        //     id: $attrs.id,
                        //     swfUrl: wamiSettings.swfUrl,
                        //     onReady: function () {
                        //         console.log('made it');
                        //     }
                        // })
                    }
                });
            }
        };
    }]);

// Define the native WAMI service
// This code is copy and pasted from https://code.google.com/p/wami-recorder/source/browse/example/client/recorder.js

var Wami = window.Wami || {};

Wami.createID = function() {
    return "wid" + ("" + 1e10).replace(/[018]/g, function(a) {
        return (a ^ Math.random() * 16 >> a / 4).toString(16)
    });
}

// Creates a named callback in WAMI and returns the name as a string.
Wami.nameCallback = function(cb, cleanup) {
    Wami._callbacks = Wami._callbacks || {};
    var id = Wami.createID();
    Wami._callbacks[id] = function() {
        if (cleanup) {
            Wami._callbacks[id] = null;
        }
        cb.apply(null, arguments);
    };
    var named = "Wami._callbacks['" + id + "']";
    return named;
}

// This method ensures that a WAMI recorder is operational, and that
// the following API is available in the Wami namespace. All functions
// must be named (i.e. cannot be anonymous).
//
// Wami.startPlaying(url, startfn = null, finishedfn = null, failedfn = null);
// Wami.stopPlaying()
//
// Wami.startRecording(url, startfn = null, finishedfn = null, failedfn = null);
// Wami.stopRecording()
//
// Wami.getRecordingLevel() // Returns a number between 0 and 100
// Wami.getPlayingLevel() // Returns a number between 0 and 100
//
// Wami.hide()
// Wami.show()
//
// Manipulate the WAMI recorder's settings. In Flash
// we need to check if the microphone permission has been granted.
// We might also set/return sample rate here, etc.
//
// Wami.getSettings();
// Wami.setSettings(options);
//
// Optional way to set up browser so that it's constantly listening
// This is to prepend audio in case the user starts talking before
// they click-to-talk.
//
// Wami.startListening()
//
Wami.setup = function(options) {
    if (Wami.startRecording) {
        // Wami's already defined.
        if (options.onReady) {
            options.onReady();
        }
        return;
    }

    // Assumes that swfobject.js is included if Wami.swfobject isn't
    // already defined.
    Wami.swfobject = Wami.swfobject || swfobject;

    if (!Wami.swfobject) {
        alert("Unable to find swfobject to help embed the SWF.");
    }

    var _options;
    setOptions(options);
    embedWamiSWF(_options.id, Wami.nameCallback(delegateWamiAPI));

    function supportsTransparency() {
        // Detecting the OS is a big no-no in Javascript programming, but
        // I can't think of a better way to know if wmode is supported or
        // not... since NOT supporting it (like Flash on Ubuntu) is a bug.
        return (navigator.platform.indexOf("Linux") == -1);
    }

    function setOptions(options) {
        // Start with default options
        _options = {
            swfUrl : "Wami.swf",
            onReady : function() {
                Wami.hide();
            },
            onSecurity : checkSecurity,
            onError : function(error) {
                alert(error);
            }
        };

        _options = angular.extend({}, _options, options);

        // Create a DIV for the SWF under _options.id

        var container = document.createElement('div');
        container.style.position = 'absolute';
        _options.cid = Wami.createID();
        container.setAttribute('id', _options.cid);

        var swfdiv = document.createElement('div');
        var id = Wami.createID();
        swfdiv.setAttribute('id', id);

        container.appendChild(swfdiv);
        document.getElementById(_options.id).appendChild(container);

        _options.id = id;
    }

    function checkSecurity() {
        var settings = Wami.getSettings();
        if (settings.microphone.granted) {
            _options.onReady();
        } else {
            // Show any Flash settings panel you want:
            // http://help.adobe.com/en_US/FlashPlatform/reference/actionscript/3/flash/system/SecurityPanel.html
            Wami.showSecurity("privacy", "Wami.show", Wami
                    .nameCallback(_options.onSecurity), Wami
                    .nameCallback(_options.onError));
        }
    }

    // Embed the WAMI SWF and call the named callback function when loaded.
    function embedWamiSWF(id, initfn) {
        var flashVars = {
            visible : false,
            loadedCallback : initfn
        }

        var params = {
            allowScriptAccess : "always"
        }

        if (supportsTransparency()) {
            params.wmode = "transparent";
        }

        if (typeof console !== 'undefined') {
            flashVars.console = true;
        }

        var version = '10.0.0';
        document.getElementById(id).innerHTML = "WAMI requires Flash "
                + version
                + " or greater
https://get.adobe.com/flashplayer/";

        // This is the minimum size due to the microphone security panel
        Wami.swfobject.embedSWF(_options.swfUrl, id, 214, 137, version, null,
                flashVars, params);

        // Without this line, Firefox has a dotted outline of the flash
        Wami.swfobject.createCSS("#" + id, "outline:none");
    }

    // To check if the microphone settings were 'remembered', we
    // must actually embed an entirely new Wami client and check
    // whether its microphone is granted. If it is, it was remembered.
    function checkRemembered(finishedfn) {
        var id = Wami.createID();
        var div = document.createElement('div');
        div.style.top = '-999px';
        div.style.left = '-999px';
        div.setAttribute('id', id);
        var body = document.getElementsByTagName('body').item(0);
        body.appendChild(div);

        var fn = Wami.nameCallback(function() {
            var swf = document.getElementById(id);
            Wami._remembered = swf.getSettings().microphone.granted;
            Wami.swfobject.removeSWF(id);
            eval(finishedfn + "()");
        });

        embedWamiSWF(id, fn);
    }

    // Attach all the audio methods to the Wami namespace in the callback.
    function delegateWamiAPI() {
        var recorder = document.getElementById(_options.id);

        function delegate(name) {
            Wami[name] = function() {
                return recorder[name].apply(recorder, arguments);
            }
        }
        delegate('startPlaying');
        delegate('stopPlaying');
        delegate('startRecording');
        delegate('stopRecording');
        delegate('startListening');
        delegate('stopListening');
        delegate('getRecordingLevel');
        delegate('getPlayingLevel');
        delegate('setSettings');

        // Append extra information about whether mic settings are sticky
        Wami.getSettings = function() {
            if (recorder.getSettings) {
                var settings = recorder.getSettings();
                settings.microphone.remembered = Wami._remembered;
                return settings;
            }
            else {
                return null;
            }
        }

        Wami.showSecurity = function(panel, startfn, finishedfn, failfn) {
            // Flash must be on top for this.
            var container = document.getElementById(_options.cid);

            var augmentedfn = Wami.nameCallback(function() {
                checkRemembered(finishedfn);
                container.style.cssText = "position: absolute;";
            });

            container.style.cssText = "position: absolute; z-index: 99999";

            recorder.showSecurity(panel, startfn, augmentedfn, failfn);
        }

        Wami.show = function() {
            if (!supportsTransparency()) {
                recorder.style.visibility = "visible";
            }
        }

        Wami.hide = function() {
            // Hiding flash in all the browsers is tricky. Please read:
            // https://code.google.com/p/wami-recorder/wiki/HidingFlash
            if (!supportsTransparency()) {
                recorder.style.visibility = "hidden";
            }
        }

        // If we already have permissions, they were previously 'remembered'
        if (recorder.getSettings) {
            Wami._remembered = recorder.getSettings().microphone.granted;
        }

        if (_options.onLoaded) {
            _options.onLoaded();
        }

        if (!_options.noSecurityCheck) {
            checkSecurity();
        }
    }
}


text:
The intensity of the reaction recorded in dogs with a positive DTH response (median) was 9
Genomics Proteomics and Bioinformatics > GLP1 Receptors > The intensity of the reaction recorded in dogs with a positive DTH response (median) was 9
admin July 6, 2022 GLP1 Receptors
The intensity of the reaction recorded in dogs with a positive DTH response (median) was 9.5 mm (8.5 to 11 mm) against VirB7 and 12 mm (6.5 to 25 mm) against VirB9. be also Mcl1-IN-1 elicited in dogs. INTRODUCTION Brucellosis is caused by Gram-negative bacteria of the genus S19, RB51, and Rev-1. While these vaccines have reduced the virulence for animals, they are able to create disease in human beings still, as demonstrated from the event of brucellosis instances because of vaccine strains among veterinarians and additional risk organizations (7,C9). Live vaccines likewise have limited use in pets given that they can stimulate abortion in pregnant females. Because of these dangers, many researchers possess investigated alternate vaccination approaches for brucellosis, like the usage of subunit vaccines predicated on recombinant protein (10,C12) or the usage of DNA vaccination (13,C15). varieties are intracellular bacterias that establish their favored replication market in macrophages (16, 17). Because of this intracellular area, gamma interferon (IFN-), created primarily by T helper 1 (Th1) and Compact disc8+ lymphocytes, offers been shown to become of central Mcl1-IN-1 importance for the control of disease through its capability to activate the bactericidal features of macrophages (18). For this good reason, many reports on applicant vaccines for brucellosis possess centered on the induction from the immune system responses resulting in IFN- creation (10, 13, 19). Among the crucial virulence elements mediating the intracellular success of different varieties may be the type IV secretion program (T4SS), encoded from the VirB operon (to genes) (20, 21), which includes been proven to be needed for success and (20,C23). It's been postulated how the T4SS mediates the secretion of virulence elements that may donate to the ability of the bacteria to determine its replicative market (20,C22, 24). The manifestation of genes can be induced intracellularly in the 1st hours after uptake of by macrophages (24, 25). Since many brucellae die through the preliminary stage of intracellular establishment, we hypothesize that contaminated macrophages probably screen peptides produced from VirB protein in the framework of main histocompatibility complex course II (MHC-II) substances on the mobile surface. With this context, VirB-specific Th1 cells may recognize contaminated macrophages and respond using the creation of Mcl1-IN-1 IFN-, resulting in the activation of macrophagic antimicrobial systems. The primary goals of today's study had been to assess if the induction of the Th1-type immune system response against VirB proteins may shield mice from disease and whether this sort of response could be induced in your dog, a natural sponsor for stress JM109 (Promega, Madison, WI) was utilized as the sponsor for propagation of plasmids. Stress BL21(DE3) (Stratagene, La Jolla, CA) was useful for expression from the recombinant protein. Bacterial strains had TSPAN2 been routinely expanded at 37C in Luria-Bertani (LB) broth or agar, supplemented when needed with 100 g/ml of ampicillin. The plasmids pTrcHis-FusB7 AR and pTrcHis-FusB9 AR including the VirB9 and VirB7 genes, respectively, with the help of a poly(H) tail, had been supplied by Diego Comerci kindly, UNSAM, Argentina. Skilled BL21(DE3) colonies had been changed with these plasmids. Ampicillin-resistant colonies including the pTrcHis-FusB7 AR plasmid had been expanded in Mcl1-IN-1 Terrific broth moderate including 100 g of ampicillin/ml at 37C with agitation (160 rpm) until achieving an optical denseness at 600 nm (OD600) of just one 1.0. Five milliliters of the tradition was diluted to 500 ml and cultivated until achieving an OD600 of just one 1.0. After addition of just Mcl1-IN-1 one 1 mM isopropyl–d-thiogalactopyranoside (IPTG) to stimulate VirB7 protein manifestation, bacteria had been incubated for more 4 h. Bacterias had been pelleted by centrifugation (15,000 for 30 min at solubilized and 4C in a remedy including 100 mM NaH2PO4, 10 mM Tris-HCl, and 8 M urea (pH 8.0) in 4C overnight with agitation. After centrifugation (20,000 amebocyte assay (Affiliates of Cape Cod, Woods Opening, MA), was 0.25 endotoxin unit/g protein. The proteins concentrations from the antigen arrangements were dependant on the bicinchoninic acidity technique (Pierce, Rockford, IL) using bovine serum albumin as the typical. strains. 544 (soft virulent stress), and an area medical isolate of had been from our lab collection. Bacteria had been cultured in tryptose-soy agar (Merck, Buenos Aires, Argentina) supplemented and incubated as referred to previously (11, 26). stress manipulations had been performed in biosafety level 3 services. Tests in mice..


This is a follow-up to my post: What Is A Software Company?
A software company's job is to distribute the cost of making the software over many customers. If this is true then acquiring as many customers as possible is important to the company. The more customers you acquire the less you can sell your software for. If your software costs a million dollars to develop and you have ten customers you need to sell it for $100,000 per customer. However, if you have 100,000 customers, you can sell it for $10 per customer (somewhere you need profit).
Being able to sell it for less means you make it harder for your competition. If both companies require a million dollars to make the software and you have 100,000 customers and they have 10 customers, you will have very different price points, making it hard for them to reach profitability or acquire the next customer. It also makes it very hard for another company to enter the market. Because of the market dominance effect of software, we see a lot of new software companies enter emerging markets, trying to acquire enough customers early in the new technology space.
Lots of customers means that the software must solve the needs of all the customers, which means broad generalized software with lots of features. However we are seeing profitable software companies appearing that have more specialized software that caters to a market niche. How is this possible? It is because they have keep their expenses low.
It is less expensive to make software now than in anytime in history. Technologies like MVC, .NET, Ruby on Rails, The Internet (for distribution), Forums/Wikis/Video Streaming (for documentation), Flash/Silverlight/HTML (for platform) and MSI (for windows installation) have greatly decreased the costs of making software. Customers want to buy software that is more specialized to what they are doing – it makes their jobs easier. Before this software was expensive, an example being television script writing software (specialized) as compared to Microsoft Word (generalized), lower costs for software companies have brought down the prices for specialty software.
Seth Godin wrote a book called Purple Cow where he stressed that in order to market your business you needed to be remarkable. One type of remarkable software is software that feels like custom software (developed specifically for a customer), however is priced like generalized commercial software. Successful new software companies are emerging that take the Purple Cow philosophy and making specialized software for niche markets by using new technologies to keep their costs down. This is Purple Software.


I am here to share my experience in flying to Costa Rica via Europe through KLM. I chose this airline for the reason that I don't need to get any transit visa at all. The downfall is the long hours of trip because of layover in each transit and the cost which is expensive for me where I can get much cheaper flight from another airlines via USA or another part of the world. I just hate processing papers for visa so, this is the best for me.
In my first trip going to Costa Rica in the year 2014, I booked my round trip tickets through phone call using the local phone in the Philippines. The agent was a Filipino so, it was easy for me to communicate and present my queries in tagalog because I had a lot of questions about transits. It was so expensive for me when I booked it a week or few days before my flight. I chose 28 days for my return date and it costs me almost $3000 USD. This is good for 1-year if you opt to change your flight especially the return ticket. It is allowed to re-book your tickets but it should be one day before your departure because there would be penalty you gonna pay if you missed your flight or you did not show up on the day of your flight then, you wanted to re-book it on your preferred date after you missed the flight.
This is also non-transferable nor refundable. They provide good meals and you will have 20kg baggage allowance. The travel tax is also included in the ticket so, you don't need to pay the tax collector at the PH airport.
* Cancellation is not possible.
* Re-booking before and after departure is permitted at a fee of $120 USD plus fare difference, the ticket may change due to tax calculation. There is a service charge if you change via telephone.
* No refund if you decide to cancel.
* Cancellation and re-booking of return ticket is not possible.
The departure time is at night but the check-in time is at 4pm. During the check-in, the agent/officer/crew (whatever you call them) at the entrance will ask you to show your ticket and passport. She will also ask you some personal questions about your destination and purpose of travel. My first trip took longer than normal, they even question why I took big luggage with me if I plan to stay in CR for only 28 days. For Filipino travelers and first timer, you should know how to answer them confidently as if they don't have the right to question your rights to travel.
In my second trip, it was smooth and relaxed. The flow is like with OFW passengers with no questions after showing their papers. They will put sticker at the back of your passport cover and jot down your names in their notebook. In the counter, they told me to go get the clearance of my daughter at the Philippine travel tax collection section after they printed out something to show to the PH travel tax collector. Then, we returned to the counter to give that clearance and they gave us our boarding passes (3-boarding pass: PH, Amsterdam, Panama). Our luggage will be picked up at the CR airport and laptop is not allowed in the baggage, it should be hand-carry.
You need to pass through the immigration section for exit stamp. Take with you the filled-out departure card (you can get at the entrance), passport and other documents related to your trip. After getting the stamp, you may pass the machine to check all your things, here you will need to take off your watch, sling bag, coins in your pocket, jacket and shoes. Water or any liquid in your hand carry is not allowed. Then, you are free to go to your boarding gate at 111. If you still have a lot of time, you can do shopping or visit restaurants here when hungry before going to your assigned gate.
At the entrance of the gate, they will ask you to show your boarding pass and passport. In my case, they put me in priority lane because I have a kid and I'm pregnant. This priority is only for passengers with Blue Miles card or membership and mostly rich can afford this service because they travel a lot through this airline. Meaning, they can be the first to board in the plane among other passengers and we were lucky to experience that without availing membership. Then, you are ready to fly!
The first transit will be in Amsterdam but the flight has one stop at Taipei for an hour or so. Here, they will clean up and refuel the plane before proceeding to Amsterdam. You will need to take your bags with you in disembarking the plane. After that, someone will give you pass card (yellow in color) and you need to hold that until you board the plane again. You will pass through the machine again to check your belongings. Then, you are ready to go to the gate, you will need to follow your fellow passengers but there are still stewardess on the way to guide you. When you reach the gate, same process will be applied, priority lane on left and on the other side are those with no Blue Miles. They have charging area in this gate so, this is your chance to use the airport amenities. After an hour or so, the passengers will go back to the same seat # assigned when leaving PH. Then, you are ready to fly to Amsterdam!
At Amsterdam airport, security is so strict. They have monitor or big screen to check your boarding gate. There's no boarding gate written in your boarding pass so, better check that in the monitor or ask the officer on the lobby. You will enter again in the machine to check your belongings. No water or liquid allowed so, throw it in the trash bin before entering. After this, you will turn to your right side to go to your gate. But, better ask the officer to give you the correct direction. They can check all the flights through their phone and very helpful. In this transit, you don't need to pass through immigration. Just go straight to the boarding gate. The airport is so clean and nice. From the toilet to the waiting area. The amenities are so good from free use of computer and wifi spot. I really enjoyed my stay here while waiting for my next flight. You can choose bench, chair, sossy sofa and bed while hanging out in there. Same procedure in boarding the plane again going to Panama. Just show your passport and boarding pass to the entrance. I got priority treatment again.
In this transit, it will be hard for you to know your gate because most of the officers you will meet here cannot speak English, only Spanish. You need to go directly to Copa Airlines to ask your boarding gate number. No more priority experience, they will call according to your seat rows. But, I still moved fast to be in front of the line and the stewardess can't do anything about it because I'm with my kid and pregnant. The over all flight experience was good. The stewardess will distribute a form to be submitted in the immigration at the arrival of CR called Registro Migratorio. So, prepare your pen.
I think this all for today! I hope I gave you a lot of information in flying to Costa Rica throuh KLM Airline. This was my personal experience, I hope you enjoyed it! If you have questions, just leave a comment below.
Hi Robelyn! My wife Jaunice is from the Philippines. She is now in Saigon Vietnam on a tourist visa. The visa is only to June 1st. Can she travel to Costa Rica via KLM from Vietnam? Or does she need to return to the Philippines for her flight to Costa Rica?
Hi Robelyn! My wife Jaunice is from the Philippines. She is now in Saigon, Vietnam on a tourist visa. The visa is only to June 1st. Can she travel to Costa Rica via KLM from Vietnam? Or does she need to return to the Philippines for her flight to Costa Rica?
Sorry for the late response. She can check the KLM website if there's a flight from Vietnam to Costa Rica. There's another Filipina who inquired here but she's coming from Hongkong. She got a flight from Hongkong to Costa Rica from another airlines passing through Paris and Panama that don't require transit visa.
Hi Robelyn,Plano ko kc mgvidit codta Rica required pb ako transit visa in Paris?
Thanks for visiting my site. I'm glad you are planning to visit Costa Rica unfortunately, you will need a transit visa in Paris. Regards!
i am a filipina, and i am planning to visit costa rica. my partner is from canada and we are gonna meet in costa rica. my question is, do i need a transit visa if my flight has to stop in some other countries?
hope to hear back from you. thank you! have a nice day!
Thanks! Depende sa country kung saan ka magtatransit. Mula Ph to Taiwan, Amsterdam and Panama as transits walang transit visa. Kung Paris merong transit visa. So, depende saang bansa ang transit mo like US may transit visa rin. Have a nice day!
Kung ung flight po is from manila and layover po s amsterdam then to panama, may transit visa rin po ba na inquired? Hope to hear back from u. Thanks!
Walang transit visa kase dederetso ka sa gate mo hindi ka na dadaan ng immigration pagdating mo sa layover mo.


2018-07-02 : Updated: 2018-07-12 16:37:05 What Constitutes Refusal to Submit to Chemical Testing in Pennsylvania?
2017-08-10 : Updated: 2017-08-10 19:43:55 Pennsylvania's New Ignition Interlock Law Is Finally Here!!!!!!
2016-04-04 : Updated: 2016-04-04 09:31:28 Penalties for first DUI Arrest. Am I going to Jail?
2014-10-01 : Updated: 2015-11-13 17:24:51 Anatomy of a Pennsylvania DUI Offense: Is Proof of "Impairment" Required in Pennsylvania "Drugged Driving" Cases?
2014-08-26 : Updated: 2015-11-13 17:27:48 Anatomy of a Pennsylvania DUI Offense: What Constitutes "Drugged Driving," or Driving Under the Influence of a Controlled Substance?
2014-01-21 : Updated: 2018-09-11 19:02:20 Anatomy of a Pennsylvania DUI Offense: How is "Impairment" Defined?


text:
There have been a lot of inventions till now and many more are still on the table. However, there have been a lot of most useful inventions that have proved to be a boon to mankind. So, here is a list of some most useful inventions till date.
1 Some Most Useful Inventions
3 Television
4 Dry Iron
5 Refrigerator
8 Telephone
9 Light Bulb
10 Electric Kettle
11 Vaccination
12 Computer
13 Printing Press
14 Compass
15 Internet
16 Smartphone
17 Optical Lenses
19 Microscope
20 Calculator
21 Clock
22 Thermometer
23 Weighing Scale
24 Sewing Machine
25 Synthetic Dye
26 Polyester
27 The Bottom Line
Some Most Useful Inventions
When we talk about incredible and most useful inventions, there are many. There is a huge number of inventions that one cannot count. From generations to generations, there are a lot of people constantly working on things to make our lives better. In every field, we get a profound number of inventions, may it be in the field of medical science, engineering or at home. So, here is a list of such innovations that not only benefited them, but also to everyone.
Washing machine has become one of the most useful inventions since last decade. Not only does it wash clothes properly, but the labor from washing is also prevented.
Who doesn't have a TV at their home ? Television has become an integral part of our lives. Moreover, it is an invention that brings everyone together.
Can you imagine someone walking to an important event with wrinkled clothes ? If not, then trust me, dry iron is one of the best and most useful inventions.
Refrigerator is something that keeps food items fresh and makes them last long. Nowadays, every household has a refrigerator, which is very useful.
The best way for locomotion within city or intercity vehicles is definitely one of the most useful inventions of mankind. For instance, bus, car, cycle, auto, etc. fall under this category.
A common thing found in almost every home, is a fan which has proven to be one of the greatest and most useful inventions in the world.
Who doesn't want to stay in touch ? Telephone is such an invention that makes distances closer than they appear, which brings people closer no matter where they are.
One of the powerful and most useful inventions that helped us get through the darkness and brought light in our lives is an electric bulb.
Electric kettle is undoubtedly a marvelous creation for mankind. Are you someone who is afraid of using a gas stove ? If so, then this product is for you. With just a click, you can make your tea or boil water with this.
Edward Jenner was the father of vaccination. It made it easy for doctors to treat patients. Furthermore, vaccination is now available for lots of diseases and it builds immunity against the virus from entering our body.
We can never thank Charles Babbage for his incredible invention of the computer. There are plenty of activities that we can perform on a computer. From arithmetic calculations to logical operations, it can perform them all.
Printing press is indeed an amazing invention as it saves time and labor. Earlier, people were bound to write thousands of papers, leading to immense manpower. Now, one can get multiple copies of something with just a button click.
Are you someone who feels lost while going trekking or on a road trip with your friends ? If so, then you should definitely get a compass. A compass is a useful invention to let us know the directions accurately.
The Internet made everything easy and brought the world closer. Nowadays, distances between people don't really matter as long as they are able to text, call or video call each other whenever they feel like doing.
Smartphones are very useful in the present era. The last decade has seen a wide transition of smartphones, yet they are as important as anything else.
In earlier times, people used to face a lot of problems with blurry vision. But after some time, optical lenses came to light and it is still one of the most useful inventions.
A prominent thing used by almost everybody, paper is one of the greatest and most useful inventions for the planet. If it was not there, then writing wouldn't have been possible.
What is the instrument used to see the minute creatures of our planet ? Well, it's none other than a microscope. The name itself says that it is used to observe the activity of microorganisms.
Ever since calculators came to light, there is not even a single workplace where it is present for performing necessary calculations. Undoubtedly, it is a boon to mankind.
In past times, people got to know about time using sun shadows, positions and directions. Yet it wasn't always accurate because of clouds and weather changes. But now, the clock is accurate and tells time properly.
Thermometer surely deserves a position in the list of top most useful inventions. And its contribution to medical science is commendable and irreplaceable.
Weighing scale is a unique invention as it used to be difficult for people to weigh and measure the mass and weight of objects, and of course themselves as well.
Sewing machine is a powerful invention. It wasn't always easy to make clothes by sewing with hands. Thousands of people would accomodate for one piece.
Synthetic Dye
In earlier days, people used to dye clothes using natural sources. But after the invention of synthetic dye, the natural ones aren't exploited much and are useful everywhere.
Last but not the least, polyester has been one of the most useful inventions, especially in the field of fabric industry. Nowadays, lots of clothing pieces are made from this.
Inventions are a gateway to a new world. It is a door that leads to new ways or a variety of methodologies of doing or achieving something. Moreover, the aim of inventing plenty of things is only one- to make life easy and save time. Hence, it is good if we embrace the changes that these inventions bring in our lives, to make it worth the meaning.
most useful inventions
what inventions most affect your daily life
Arzoo Bhatia - September 21, 2021
Why Should You Pet a Cat?


Iovate, a manufacturer of active nutrition supplements, has started added Thinfilm's SpeedTap tags to their product packaging. The tags will be integrated into the label wrap on Iovate's Six Star Pro Nutrition®, Hydroxycut™, MuscleTech®, and Purely Inspired® brands.
The tags are integrated with Thinfilm's CNECT™ platform which will enable Iovate to create unique digital experiences including videos, recipes, instructions and product re-ordering.
Iovate's products are available worldwide and the smart label nutrition products will initially be available through Walmart stores in the US.
Interestingly, Thinfilm have offered Iovate exclusivity in the active nutrition area based on the size of the order – said to be over 1 million tags. NFC.Today spoke to Thinfilm and the tags themselves will be placed behind the label and the labels specifically printed for the campaign. This is important. Many product based NFC campaigns are fairly small scale trials. The tags are usually integrated in a temporary way and the quantities are low. Iovate aren't messing about here. The tags are fully integrated in the production process and the quantities are high. There's no question it is a good opportunity for Iovate as well. The typical sports nutrition user is likely to be information hungry, a repeat buyer and loyal to a brand. A perfect combination for NFC integration.