text:
Research note
TCC-GUI: a Shiny-based application for differential expression analysis of RNA-Seq count data
Wei Su1,
Jianqiang Sun1,
Kentaro Shimizu1,2 &
Koji Kadota ORCID: orcid.org/0000-0002-3907-43361,2
BMC Research Notes volume 12, Article number: 133 (2019) Cite this article
Differential expression (DE) is a fundamental step in the analysis of RNA-Seq count data. We had previously developed an R/Bioconductor package (called TCC) for this purpose. While this package has the unique feature of an in-built robust normalization method, its use has so far been limited to R users only. There is thus, a need for an alternative to DE analysis by TCC for non-R users.
Here, we present a graphical user interface for TCC (called TCC-GUI). Non-R users only need a web browser as the minimum requirement for its use (https://infinityloop.shinyapps.io/TCC-GUI/). TCC-GUI is implemented in R and encapsulated in Shiny application. It contains all the major functionalities of TCC, including DE pipelines with robust normalization and simulation data generation under various conditions. It also contains (i) tools for exploratory analysis, including a useful score termed average silhouette that measures the degree of separation of compared groups, (ii) visualization tools such as volcano plot and heatmap with hierarchical clustering, and (iii) a reporting tool using R Markdown. By virtue of the Shiny-based GUI framework, users can obtain results simply by mouse navigation. The source code for TCC-GUI is available at https://github.com/swsoyee/TCC-GUI under MIT license.
RNA-Seq is a common technique used to obtain gene expression data [1]. A major application of RNA-Seq data is to identify differentially expressed genes (DEGs) under different groups or conditions [2, 3]. Till date, many methods have been developed for this purpose [4,5,6,7,8,9], most of them implemented as R/Bioconductor packages [10, 11]. We had previously developed an R/Bioconductor package named TCC [7], the main characteristic of which is to implement a robust normalization procedure originally proposed by Kadota et al. [12]. It can provide accurate differential expression (DE) results especially when up- and down-regulated DEGs in one of the groups are extremely biased in their number. However, due to its limitations of usage by non-R users, there is a need of an alternative for DE analysis by TCC.
Here, we present a graphical user interface (GUI) for TCC, named TCC-GUI. Using the Shiny framework [13], it enables non-R users to manipulate the package and adjust parameters easily in order to view the DE results. The users only need a modern web browser as the minimal requirement. Contrary to the original TCC and like any other Shiny app, TCC-GUI provides plenty of visualization tools: principal component analysis (PCA) for exploratory analysis [14], Volcano plot [15] to view the DE results, and so on. While making figures with high customizability is not a trivial task even for experienced R users, TCC-GUI facilitates such a task in real-time.
TCC-GUI is built in R. The current implementation depends on the following R packages: TCC, shiny, shinyBS, shinycssloaders, shinydashboard, shinyWidgets, plotly, dplyr, DT, heatmaply, tidyr, utils, rmarkdown, data.table, RColorBrewer, knitr, cluster, and MASS. While we primarily intend to use TCC-GUI via its URL [16] without installation for non-R users, it will launch locally from any R environment where prerequisite packages are installed. Although we have provided the source code in the additional file (Additional file 1), the latest version can also be downloaded through our GitHub page [17]. The latest version can be launched as follows:
$${\text{shiny}}::{\text{runGitHub}}\left( {{\text{``TCC}} - {\text{GUI''}},{\text{ ``swsoyee''}} {\text{ subdir}} = {\text{``TCC}} - {\text{GUI''}},{\text{ launch}} . {\text{browser}} = {\text{TRUE}}} \right)$$
TCC-GUI provides a total of five steps for DE analysis (Fig. 1): data simulation (Step 0), exploratory analysis (Step 1), TCC computation (Step 2), visualization (Step 3), and report (Step 4). The minimum DE procedure only requires two steps (Steps 1 and 2). This is because Step 1 includes (i) data import and (ii) group label assignment as mandatory information for DE analysis. Step 2 is the core step of TCC-GUI application, where the DE analysis (i.e., statistical test), as implemented in TCC, is performed. The input is taken from Step 1 and DE result can be obtained in Step 2. Therefore, the remaining steps (Steps 0, 3, and 4) are not mandatory. While we will detail each step below, a tutorial for individual steps with appropriate screenshots is also provided in Additional file 2.
Analysis workflow and main functionalities in TCC-GUI. TCC-GUI provides a total of five steps (Steps 0–4), amongst which Steps 1 and 2 are mandatory
Data simulation (Step 0)
Similar to the original TCC, TCC-GUI can generate simulation data with various conditions in Step 0. The generated data can, of course, be used as input for DE analysis within TCC-GUI, as well as other tools. The "hypoData" provided as sample dataset in Step 1 is essentially the same as that generated in Step 0 with almost default settings (except for the proportion of assigned DEGs in individual groups); the total number of genes was 10,000 (Ngene = 10,000), 20% of the genes were DEGs (PDEG = 0.2), the number of groups was 2 (two-group comparison; G1 vs. G2), the levels of DE (fold-change; FC) for individual groups were fourfold (i.e., FCG1 = 4 and FCG2 = 4), the number of replicates (NR) were NRG1 = 3 and NRG2 = 3, and the proportions of assigned DEGs (P) were PG1 = 0.9 and PG2 = 0.1. Utilizing the advantage of GUI, users can recognize the number of DEGs assigned in individual groups in real-time. Simulation data for three-group comparison used in Tang et al. [18], for example, can be generated in Step 0.
Exploratory analysis (Step 1)
At the first stage in Step 1, the user will be requested to import count data and assign group information for individual samples. The user can perform exploratory analysis (quality control) based on the group labels. The exploratory analysis includes count distribution, multi-dimensional scaling (MDS), PCA, hierarchical clustering, and so on. Figure 2 shows the dendrogram of hierarchical sample clustering (SC) for "hypoData". There are two main clusters, each containing three samples belonging to the same group; this is reasonable because the data consists of two groups and contains 20% of DEGs (PDEG = 0.2).
Exploratory analysis (Step 1). A dendrogram obtained from hierarchical sample clustering for a sample dataset "hypoData" is shown. The AS score (0.246) can be seen on the left side of this figure
As a unique feature of TCC-GUI, it provides an average silhouette (AS) score for objectively estimating the degree of group separation [19, 20]. Silhouette was originally proposed for the interpretation and validation of cluster analysis [19]. Silhouettes provide a measure of how well a sample is classified when it is assigned to a cluster, based on both their tightness and the separation between them. Since the silhouette scores are calculated for individual samples, the AS value can be obtained by taking the mean across all samples.
We recently demonstrated that the AS value can be utilized for estimating the degree of group separation [20]. It ranges from − 1.0 to 1.0, and a higher AS value indicates a higher degree of group separation (i.e., a higher percentage of DEGs). In case of hypoData with PDEG = 0.2, TCC-GUI outputs AS = 0.246 (see the left side of Fig. 2). For data that contain no or few DEGs (i.e., PDEG = 0.0 approximately), the AS value would be around zero [20]. Although the AS values can be calculated independent of SC, they also provide a relevant measure for the degrees of separation between the groups of interest (e.g., G1 vs. G2) in SC results.
TCC Computation (Step 2)
This step includes data normalization and DEG identification. It provides several analysis pipelines that can be performed by changing options in the parameter setting panel (see Step 2 in Additional file 2), They include the iterative edgeR pipeline (as default), iterative DESeq 2 pipeline, and the original edgeR or DESeq 2 pipeline.
The DE results will appear in the "Result Table" panel after the operation ends. While the main output is a p-value that indicates the degree of DE between the compared groups, other information, such as adjusted p-values (i.e., q-values) and log ratio (M) values, are also provided. The user can download the complete DE results and TCC-normalized data as CSV files. The user can also extract any subset of genes by the column of interest in the table. This can be done by utilizing the boxes at the bottom of the table. For example, the user will see a range of log-ratios (= log2(G2/G1)) as [− 6.63, 6.48] in the box of the "M Value" column. The user can extract genes that are twofold higher in G2 than in G1 by setting the range appropriately, as in [1.0, 7.0].
TCC-GUI also provides R codes internally used to execute the TCC. Researchers can learn the functions that are used internally, utilize this code as a template, and obtain reproducible results.
Visualization (Step 3)
The MA plot is commonly used to visualize the DE result of two-group comparisons, by transforming the data onto log-ratio (M) scale as Y-axis and average expression (A) scale as X-axis (Fig. 3). While 1320 genes, satisfying 10% false discovery rate (FDR), are colored in dark red by default, both the cut-off value and color may be changed by the user (see Step 3 in Additional file 2). Owing to the interactive GUI framework, the user can obtain more detailed information about a gene of interest (e.g., "gene_562") by placing the cursor in that location (see the right side of Fig. 3).
Visualization (Step 3). As a representative visualization of DE result, the MA plot for two-group comparison, using "hypoData", is shown. By hovering data point (i.e., gene_562) of interest in the scatter plot, the expression pattern across samples can be seen on the right side
Based on the definition of FDR, the 1320 genes satisfying 10% FDR are theoretically composed of 1320 × 0.1 = 132 non-DEGs while the remaining 1320 × 0.9 = 1188 are true DEGs. Similarly, 1841 genes satisfying 40% FDR are composed of 1841 × 0.4 = 736.4 non-DEGs and 1841 × 0.6 = 1104.6 true DEGs. Although genes satisfying arbitrarily defined FDR cut-offs are usually defined as DEGs, an increase in the number of genes by loosening the FDR cut-off does not necessarily indicate an increase in the number of true DEGs [20]. In TCC-GUI, the number of genes satisfying various FDR thresholds can be interactively seen in the "MA Plot Parameters" panel. In addition, information of FDR cut-offs in increments of 0.05 is also provided in tabular form. This information, as well as the AS value, would be helpful to estimate how the true DEGs are included in the input data.
TCC-GUI provides Volcano plot as another way to visualize the DE result of two-group data. In contrast to MA plots that are constructed by plotting the M values (Y-axis) vs. A values (X-axis), it plots M values on the X-axis and statistical significances as − log10(p-value) on the Y-axis. Many users will be interested in genes located in the upper left or upper right areas in the plot. The user can, of course, change the colors and cut-off values for both axes and see the number of genes satisfying both the cut-offs. In case of hypoData with default settings, the user will see 1374 genes down-regulated and 283 genes up-regulated in G2. This is quite reasonable because the hypoData contains 1800 genes down-regulated and 200 genes up-regulated in G2.
TCC-GUI also provides two other visualization tools for somewhat general purposes: "Heatmap" and "Expression Level". "Heatmap" is a graphical representation of data where the individual count values contained in a matrix are represented as pseudo-colors. Hierarchical clustering is usually performed on heatmap, enabling users to interpret the overall picture of expression patterns with ease [21]. "Expression Level" can be used to visualize expression patterns for genes of interest. It would be useful to visualize, for example, expression patterns of top-ranked DEGs obtained from two- or more-group comparison.
Report (Step 4)
Although TCC-GUI has the option to export results after every step, some users may prefer the output merged into one single file. Like other sophisticated GUI-based tools, such as PIVOT [22], TCC-GUI supports this functionality in the final Step 4.
Representative analysis of a real count dataset
Here, we demonstrate a representative analysis of a real count dataset [23], available at the ReCount website [24]. The dataset consisted of 36,536 genes × 21 liver samples. Bottomly et al. [23] had studied the expression levels of two common inbred mouse strains used in neuroscience research, i.e., 10 C57BL/6J strains and 11 DBA/2 J strains. TCC-GUI displayed the results of two-group comparison: (i) the AS value was 0.187 in Step 1, (ii) 22,604 low-count genes were filtered (i.e., 36,536-22,604 = 13,932 genes were used as input for TCC computation in Step 2), and (iii) 1530 genes satisfying 10% FDR (i.e., PDEG = 10.98%) were detected as DEGs after TCC computation in Step 2. These values were exactly the same as those described in Zhao et al. [20]. A series of screenshots for this analysis is given in Additional file 3.
TCC-GUI is a browser-based application for DE analysis of RNA-Seq data. It enables non-R users to perform the TCC package without installation. In addition to the functionalities originally implemented in TCC, TCC-GUI provides plenty of interactive visualization functions. The powerful in-built functions would also be satisfactory for experienced R users.
While the development is complete from the end-user perspective, the internally used R codes are still cluttered. Moreover, the GUI in Step 4 is still in need of further improvement. These refinements are desirable in near future.
average expression
average silhouette
DEG:
differentially expressed gene
FC:
fold-changes
FDR:
false discovery rate
GUI:
multi-dimensional scaling
N gene :
total number of genes
NR :
number of replicates
PCA:
SC:
sample clustering
TCC:
Tag Count Comparison
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WS developed the TCC-GUI software and drafted the manuscript. JS provided critical comments as the first author of TCC's paper. KS designed the study, supervised the critical discussion and revised the paper. KK made the conception of the software, substantially revised the paper, and led this project to completion. All authors read and approved the final manuscript.
We would like to thank Editage (http://www.editage.jp) for English language editing.
All data in this study were included in this article.
This study was supported by JSPS KAKENHI [Grant Numbers JP15K06919 and JP18K11521]. The funding body had no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan
Wei Su, Jianqiang Sun, Kentaro Shimizu & Koji Kadota
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan
Kentaro Shimizu & Koji Kadota
Wei Su
Jianqiang Sun
Kentaro Shimizu
Koji Kadota
Correspondence to Koji Kadota.
Source code for TCC-GUI. This file can be used to launch TCC-GUI locally. The primary aim is to provide reproducible results described in the manuscript.
Tutorial for TCC-GUI. A step-by-step instruction to perform individual steps for TCC-GUI is provided.
Representative analysis of Bottomly's dataset. A series of screenshots while analyzing Bottomly's real count dataset is provided.
Su, W., Sun, J., Shimizu, K. et al. TCC-GUI: a Shiny-based application for differential expression analysis of RNA-Seq count data. BMC Res Notes 12, 133 (2019). https://doi.org/10.1186/s13104-019-4179-2
Received: 14 January 2019
Differential expression analysis


LI Tai-Xiang was born in Malan, Taitung in 1941. He was mesmerized by the music played by his elementary school teacher, Lin Fu-Yu. In junior high school, he met a teacher named Chen Gang-Qing, who quickly fostered Li's violin talents. In 1956, Li began his studies at National Taiwan University of Arts as a fine arts printing major. He eventually transferred to the music department and became a violin major. Hsu Tsang-Houei became his spiritual guru and changed his entire life. He taught in his hometown of Taitung for five years after graduating, and then went back to Taipei to develop his career. He was awarded a Wu San-Lien Award, as well as a Golden Horse Award for Best Original Film Song for "The Sword of Romance." He died in Xindian on Jan. 2, 2014.


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text:
A potent botnet is exploiting a critical router bug that may never be fixed
Dan Goodin
Categories: Biz & IT
View non-AMP version at arstechnica.com
A Dasan Networks router similar to this one is under active exploit by the potent Satori botnet.
Dasan Networks
A fast-moving botnet that turns routers, cameras, and other types of Internet-connected devices into potent tools for theft and destruction has resurfaced again, this time by exploiting a critical vulnerability that gives attackers control over as many as 40,000 routers. Despite the high stakes, there's no indication that the bug will be fixed any time soon, if at all.
Satori, as the botnet has been dubbed, quickly made a name for itself in December, when it infected more than 100,000 routers in just 12 hours by exploiting critical vulnerabilities in two models, one made by Huawei and the other by RealTek. Last month, Satori operators released a new version that infected devices used to mine digital coins, a feat that allowed the attackers to mine as much as $3,000 worth of Ethereum, based on prices the digital coin was commanding at the time.
In recent days, Satori has started infecting routers manufactured by Dasan Networks of South Korea. The number of daily infected routers is about 13,700, with about 82 percent of them located in Vietnam, a researcher from China-based Netlab 360 told Ars. Queries on the Shodan search index of Internet-connected devices show there are a total of more than 40,000 routers made by Dasan. The company has yet to respond to an advisory published in December that documented the code-execution vulnerability Satori is exploiting, making it possible that most or all of the devices will eventually become part of the botnet.
"We tried to contact Dasan since October 8, 2017," researchers from vulnerability disclosure service SecuriTeam wrote in the December 6 advisory. "Repeated attempts to establish contact went unanswered. At this time, there is no solution or workaround for this vulnerability." In an email sent Wednesday, Noam Rathaus, CTO of SecuriTeam's parent company Beyond Security, wrote:
We tried to contact Dasan several times since October. By "several times" I mean probably over 10 emails, several phone calls, and requests to both their support and their sales departments.
Since we were aware that there may be a possible language barrier, we went as far as having the head of our Korean office send them the full explanation in Korean with an invitation to communicate directly with us to coordinate the disclosure; our Korean office tried to contact them via email and over the phone but, except for a short confirmation that they have received our communication, we never got any updates.
Attempts by Ars to contact Dasan representatives weren't immediately successful.
Nearly endless supply of vulnerabilities
Satori is based on Mirai, the open source Internet of Things malware that powered a series of botnets that delivered record-breaking distributed denial of service attacks in 2016 and debilitated core parts of the Internet for days. Unlike thousands of other Mirai variants, Satori featured a key improvement. Whereas Mirai and its imitators could infect only devices that were secured with easily guessed default passwords, Satori exploited firmware bugs, which often go unpatched, either because of manufacturer negligence or the difficulty device owners face in patching their devices.
"The Satori developer is actively updating the malware," Netlab 360 researcher Li Fengpei wrote in an email. "In the future, if Satori makes more headlines, we will not be surprised."
Like most IoT malware, Satori infections don't survive a device reboot. That means the December infections of the Huawei and RealTek devices—which Netlab 360 estimates totaled 260,000—are largely gone. The botnet, however, has managed to persist thanks to a nearly endless supply of vulnerabilities in other IoT devices. Besides the infection methods already mentioned, Satori has also managed to spread by exploiting flaws in custom versions of the GoAhead Web server that are embedded in wireless cameras and other types of IoT devices, researchers from security firm Fortinet reported two weeks ago. A GoAhead spokesman told Ars: "The flaw isn't really in the implementation of the web server, but in the web application that uses it, i.e. just because a device has GoAhead does not mean it is vulnerable."
Pascal Geenens, a researcher at security firm Radware who reported the new Satori variant on Monday, told Ars it's not entirely clear what the purpose of the botnet is. Last month's variant, mentioned earlier, that infected the Claymore Miner software for generating cryptocurrency may provide a key clue. The variant, Geenens said, is a strong indication that Satori operators want to steal digital coins or computing resources used to generate them. He said both the Claymore and Dasan variants rely on the same command-and-control infrastructure and that the word Satori is included in the binary files of both versions.
Piotr Bazydło, a researcher at the NASK Research and Academic Computer Network, told Ars that he believes the new variant may have infected as many as 30,000 routers so far and that Satori developers likely have plans for new attacks in the near future.
"I guess they are trying to follow the trend and provide a botnet for cryptocurrency mining/stealing," he wrote in an email. "People should be aware that there may be more variants of Satori in the future, [and] thus other IoT devices may be targeted."
This post was updated on 2/15/2018 to add comment from GoAhead.
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redpajama_set_name: RedPajamaCommonCrawl


This Gameboy survived a bomb blast.
They don't call it "The Brick" for nothing.
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text: Q: Firebase notification not showing emoticons in notification when app is in background or closed when the message has unicode of emoticons like \u{1f600}, the notification will display emoticons, but when the application is in the background, notifications only shows unicode , not emoticons, I have searched the entire internet about this, I really need to show emoticons even though the application is in the background, any help I will really appreciate , thank you.

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The club season may be over as we get ready for Christmas but there has still been plenty of snaps from the world of golf to choose from for our November month in pictures.
A baboon and his offspring were spotted as the European Tour arrived in South Africa, Pat Perez may have won in Mexico but he's winning no points for his hair do, and players at the Carya course in Turkey had to drive off the apartment of a roof on the 16th tee.
Check out these great pictures in our month in pictures gallery.


Neon Smoke is a Gord Bamford album through and through with familiar melodies, lyrics and sound. If you're a Gord Bamford fan, you're going to love all 15 tracks. It's very reminiscent of his last three albums: Tin Roof, Country Junkie, and Is It Friday Yet?
The risk with this album is that it likely won't do much to attract new fans to the Gord Bamford army, or convert those who sit in the middle of his fandom (those who don't love him, don't hate him). That being said, his fans are fierce and are so supportive of him and his career – so really, why try to fix something that ain't broke?
The title track Neon Smoke is definitely the standout single and will likely do well for him in terms of airplay and fan support. Another notable track is Ain't It Grand featuring Blue Rodeo's Jim Cuddy. It's a bit of a Canadian anthem that pegs Canada versus the US with lyrics like "Couple Tims' in the console", "We got the Blue Jays", and "We got the strongest beer". It's a fun song, and will be fun to play at your Canada Day party this year.


"Minimizing Your Footprint, Enhancing Your Performance"
While planting may seem straightforward, the challenges posed by funding availability, specimen quality, and the needs of your community can quickly complicate even the smallest of planting programs. Since tree planting can often require a significant investment it's important to make sure it's done right. At EC Consulting, we have extensive experience in tree planting, covering every aspect from selecting each location to writing the contracts to ensure the highest quality of workmanship.
Whether you're looking to redesign a downtown shopping area, re-plant a park, add shade trees to your town, or simply plant a specimen tree, we've got you covered. Our arborists have overseen the planting of thousands of trees from suburban NJ to the most industrial areas of NYC. This experience coupled with the knowledge of how the urban forest works allows us to tailor a planting plan for you that will help you reach all your planting goals.
Very often too little consideration is given to the long term growth of the trees to be planted. Site preparation, species selection, and maintenance expectations all need to be considered to ensure that you're getting the right tree in the right place. Ensuring the right trees are planted in the right locations will minimize your maintenance expenditures, increase the durability of your hardscapes, and maximize your investment.
If aesthetics are of primary importance, there's no need to worry. We partner with landscape architects to create plans for every aesthetic. Tall stately trees, shady alles, and groves of flowering trees are just some of the aesthetic options we offer as part of our designs.
No project is too large or too small. Let us design a planting plan for your next planting season and see what's possible with our experience at your service.
A comprehensive planting plan will enable you to make the most of your funds and efforts. Choosing the right locations, the right species, and organizing this information for maintenance and tracking is essential for the reduction of maintenance, the longevity and health of the trees, as well as getting the most for the money.
Planting plans begin as a collection of locations with specific details. These details include the exact location, the size of the prepared planting area, the species of tree to be planted, and the reason for the planting. The species that can be planted is determined by the environmental conditions and spatial requirements. For example, a planting space in front of a home under utility wires is a great place for a small flowering tree, but not a good place for a large oak. Just as a hardy medium sized tree is better for urban areas with high foot traffic than a small flowering tree that is sensitive to salt used on sidewalks in winter.
The goals of the planting will heavily influence the placement of the trees and the selection of the species. We can help you to determine the goals around your budget and plan for the most effective way to reach these goals. Creating drawings as well as easy to use spreadsheets for the tracking of the planting and health of the tree after planting will also help you to get the most out of your planting.
ⓒ 2019 by Environmental Conservation Consulting NY, LLC. All Rights Reserved. AY Creative, Inc.


Chuck Liddell held onto the UFC light heavyweight title with a fourth-round TKO win over Jeremy Horn at UFC 54.
Liddell, who had won the title four months earlier by knocking out Randy Couture, scored his 12th UFC victory to break Royce Gracie's record.
Horn returned to the UFC after racking up 31 wins during a four-year odyssey through Pride, WEC, IFC, King of the Cage and other smaller promotions.
The title fight ended when Liddell blasted Horn in the eye, and Horn told referee John McCarthy that he could not see.


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Home World Ukraine issue; India away from UN Security Council vote – News...
Ukraine issue; India away from UN Security Council vote – News 360 – WORLD
United Nations Security Council
Geneva: India avoided voting in the United Nations Security Council (UN Security Council) ahead of the meeting to discuss the Ukraine issue. At the request of the US, Russia sought a vote before the meeting. The 15-member committee needed 9 votes to go ahead with the meeting. Apart from India, Gabon and Kenya also did not participate. All other members, including France, the US and the UK, voted in favor, while Russia and China voted against.
India was of the view that conflict should be avoided and a calm and constructive diplomacy was needed. Earlier, India had said that it was closely monitoring all developments in Ukraine, including talks between Russia and the United States, and that the situation should be resolved peacefully.
Foreign Ministry spokesman Arindam Bagchi said that the Indian Embassy in Kiev is monitoring the situation in Ukraine. India's Permanent Representative to the United Nations TS Tirumurti also clarified.
Meanwhile, Russia has denied US allegations that it deployed more than 100,000 troops near the Ukrainian border at a UN Security Council meeting on Ukraine. Russia has accused the US of creating unnecessary panic over the Ukraine issue.
See also Banksy-funded rescue boat involves 'immediate assistance'
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In the human history, men are the ones with highest number of hairs on their body. The reason why this is the case is because almost 75% of a man's body is covered with hair, which can be irritating at times. Although men do not have the longest hairs in the world, hair cover on their chests, arms, legs, private parts and even on the armpits is remarkable and needs some cutting. This calls for men's hair removal procedures, with every part requiring a special way to remove these hairs.
Most men are easily agitated with the volumes and thickness of hairs on their chests, which makes most of them feel gross. Due to this reason, there are various ways of hair removal on the chests. Nevertheless, the most effective and recommended way of removing these hairs is in the use of hair clearing gels. You can find most of these in stores or even in chemists, as they are not hard to find.
However, you have to be careful when using these gels, as some of them can be harmful at times. You can take an advantage of your physicist to recommend the best gel for men's hair removal on the chests. You should only use products that have no side effects on your body, which is the reason why you might need the help of a physicist when choosing an appropriate method to remove these hairs.
Using gels to remove hairs from under arms is also a great way of getting rid of these hairs. It is advisable and healthy for a man to clear out hairs on his under arms and private parts because these hairs tend to attract sweat and other dirt materials, thus producing body odor. Body odor of any kind on men tends to drive women away from men, hence a reason why it is advisable for one to make sure he is free from the same by practicing hair removal.
Another most effective men's hair removal technique is in using a shaver to clear out hairs on the head and the beard if any. However, before you can use a shaver to shave off these hairs, you need to use a soothing lotion or powder to protect your skin from cuts and bruises. There are many before and after shave lotions and powders for you to choose in the markets. If you cut your hairs regularly to maintain your looks and profile, you then need to know one thing; these shavers get blunt easily, and needs to be sharpened most of the times. Shaving with a blunt shaver can be detrimental as it tends to pull hairs from your head, which leads to growth of pimples on the shaved areas, and this is not what you need.
Although there are many men's hair removal technics and procedures, it would be wise for you to choose a more appropriate and effective way of doing the same. When doing this, you should also consider your health and safety, and never rush out to use any product being launched every day.
Are you looking for a clinic where Permanent Hair Removal Men Treatment is offered at reasonable cost. Here's the Best place for youSkin Laser Hair Removal .
These products are formulated with the botanical and herbal tints, help in giving safe and effective tint that maintain the quality of the locks. Today, most of the people use chemical based hair colorants with an objective to give radiant and unique shade.
Doing things in a natural way as waxing and prescription types of natural materials other and To get them, imagine a soft smooth face, feet and hands are pretty clean with natural ingredients without having to go to a spa shop.


Teeth are generally predictable. We know the first permanent teeth we will get are our first molars, also known as six year molars because we get them between six and seven years old. We know they will be bigger than all our other teeth. We know our maxillary central incisors, our two top front teeth, will come in about seven to eight years old and be a particular shape and size. Wisdom teeth; however, have a mind of their own! They come any anywhere from 16-25 years old, and sometimes don't even stick to that time frame. They can be big as your other molars or small as your front teeth. They can come in properly and vertically or angled and horizontally. I have even seen X-rays of wisdom teeth going the wrong direction! They were headed back towards the jaw bone! Wisdom teeth do whatever they want.
Not enough room in your mouth.
Many of us do not have the space in our mouths to accommodate wisdom teeth. Not enough room in our mouths can lead to the wisdom teeth causing pain. It can also cause problems in keeping those teeth clean. They are often hard to reach which means plaque is allowed to grow and cause cavities, tartar, and/or gum infections.
When teeth are impacted they are not coming in the proper direction. This could be anywhere from tilted to all the way horizontal. This can present a variety of issues. The major problem can be damage to other teeth. If the wisdom tooth is tilted and now running into the back of your second molars, this can cause damage, decay, and potential loss of that second molar. Impaction can also lead to cysts or infections around those teeth. This can lead to long term damage of your jaw bone.
When teeth are partially erupted, only part of the tooth has grown into the mouth, the other is still covered with gum tissue. This can be very hard to keep clean. It is a great spot for food impaction and plaque bacteria to collect. As mentioned before, this can lead to cavities, tartar, and/or gum disease.
Size of wisdom teeth can play a big factor is health and recovery. Most dentists like to get them out after the crown is fully formed, but before the roots are complete. This helps extraction process to go easier and quicker, as well as reduces nerve damage. Wisdom teeth, particularly those on your bottom jaw, can grow around or next to the nerve that runs through your jaw. When the roots of wisdom teeth are allowed to grow close to that area, that increase risk for nerve damage upon extraction. This damage can be temporary or permanent.
Stage of jaw bone growth plays a factor. Dentist usually recommend wisdom teeth to come out in the teen years or early twenties. This is because your jaw bone is still growing. Once you hit your thirties, your bone is much more solid and recovery time after extractions can be longer and more difficult.
Why wait till it hurts? Get them out on your schedule. Spring break, summer vacation, fall break, long weekend, these are the best times for recovery. Don't put yourself through tooth pain, its an awful experience, and one that is avoidable!
You've decided to get them out! Now what?
This is a great time for a chat with your dentist. They can help you determine the appropriate avenue for you.
Just getting numb – Your appointment will be pretty straight forward. The wisdom teeth may already be erupted and properly aligned. You are not very nervous about the appointment.
Nitrous and getting numb – Nitrous, or laughing gas, can be administered before the local anesthesia. This can help some people with anxiety. It is also good to help people relax if there is a little more work involved in getting out the wisdom teeth – they are partially erupted or completely unerupted.
IV Conscious Sedation – This is a great option for a more involved procedure or those that have very high anxiety. A dentist trained in this method, will administer medication through an IV that allows you to relax. You are able to respond to questions (Can you open wider? Are you doing ok? Can you turn to the left?), but you will not remember the procedure.
Oral Surgeon Specialists – In some cases the removal of the wisdom teeth may be very complicated. This can be due to position, age, nerve involvement, etc. You may then be referred to a specialist to handle this situation.
Whatever way and method can be tailored to your specific needs through exam, xrays, and a visit with your dentist.
DRY SOCKET: The Absolute Worst.
A dry socket is a complication which occurs after about 25% of wisdom tooth extractions, and is more common in the lower jaw than the upper jaw. The incidence of a dry socket resulting from the extraction of any other teeth is considerably lower; only two to three percent. The symptoms of dry socket include a bad taste in the mouth, a dry-looking socket with white-ish bone at the bottom instead of a dark red blood clot, and pain that progressively becomes more severe and can sometimes radiate to other parts of the head and neck. Symptoms typically occur about two to three days after the extraction and are often uncontrollable with over-the-counter pain medication. If you have ever experienced a dry socket, then you already know that the pain is excrutiating. So, what exactly is a dry socket? Why does it happen, how can it be prevented, and most importantly, how can it be treated?
When a tooth is pulled, what's left behind is an empty socket site in the bone where the roots of the tooth used to be. Following the extraction, a blot clot forms in the socket site and protects the underlying bone and nerves from exposure to the mouth. Over time, the socket site heals and is filled in with new bone. If this blood clot is lost prematurely, however, the underlying bone and nerves are exposed to the environment of the mouth, which is crawling with bacteria. This exposure can lead to infection and the dreaded dry socket. Dry socket is more prevalent in the lower jaw than the upper jaw, because the lower jaw has a poorer blood supply than the upper jaw, and food debris is more likely to become trapped in socket sites due to gravity.
Following an extraction, dental professionals provide post-operative instructions in the interest of preventing a dry socket. They will advise to avoid any foods with small seeds or nuts which could get trapped in the socket site, avoid strenuous activity following surgery to prevent additional bleeding, and to avoid vigorous rinsing that could potentially dislodge the blood clot. Instruction will be given to avoid spitting or drinking through a straw for at least two or three days. Spitting and using a straw creates negative pressure in the mouth which could dislodge a clot. Your dental professional will likely also tell you to avoid the use of tobacco at all costs, especially cigarette smoking. Smoking also creates negative pressure in the mouth which could dislodge a clot, and the nicotine in the cigarette slows healing and constricts blood flow necessary for proper healing.
Besides providing helpful instructions for how a dry socket can be prevented at home, your dental health professional can also help to prevent dry socket from occurring by placing a small suture in the extraction site if necessary, and by rinsing with an antimicrobial chlorhexidine rinse immediately after the extraction. Rinsing with chlorhexidine solution after extractions has been shown to prevent about 40% of potential dry sockets.
Despite dry socket being very painful, the treatment is fairly simple. If you think you may be experiencing dry socket, call your dental provider right away. Once they determine a dry socket is present, your dental professional will clean the socket site and apply a medicated dressing. Your dental professional may administer a local anesthetic to the area to provide pain relief and to facilitate cleaning and medicating the site without causing further discomfort. The medicated dressing often includes eugenol, an ingredient which is very soothing for dry socket. Your dentist may provide a prescription for antibiotics or pain medication to assist with recovery from the dry socket. If a dry socket is left untreated, it may resolve on its own, but the pain is likely to last for about a week.
Dry socket is a very painful complication that should be avoided with every effort. If preventive efforts are made, and post-op instructions are adhered to, there's a good chance you won't experience a dry socket. If your extraction site does start to hurt after about two or three days and over-the-counter medication isn't controlling the pain, you may have a dry socket and should see your dentist as soon as possible! Dry socket is easily treatable by dental professionals.
Everyone has them, but not everybody knows what to do with them. "Wisdom teeth" or 3rd molars can cause a number of problems in the mouth.
Wisdom teeth are believed to be evolutionary and were helpful to our distant ancestors who frequently ate rougher foods. Wisdom teeth would erupt as replacements for worn out or missing teeth. Nowadays modern advancements, better hygiene, and softer diets allow us to keep most of our teeth so we really don't need wisdom teeth at all! For most people, wisdom teeth erupt in the late teen years or early 20's, usually earlier for females. They are the most posterior (towards the back) teeth. Most people have 4 wisdom teeth total, but sometimes they can be congenitally missing. Other times people may have extra (supernumerary) wisdom teeth.
Even if there is sufficient space in the mouth and the wisdom teeth are in alignment with the other teeth, they can still cause problems. Because of their location, wisdom teeth are extremely hard to brush and floss. If they are not kept clean, they can cause periodontal pocketing, which can lead to tooth loss in other areas of the mouth. Unfortunately, most people don't have enough space and when wisdom teeth erupt, they can push on the surrounding teeth. This can cause crowding and misalignment throughout the entire mouth. Wisdom teeth can also be impacted – meaning they are enclosed in the gum tissue or jawbone. When this happens they can partially erupt or even try to erupt horizontally. When teeth are only partially erupted, it allows bacteria to enter the tooth. This can lead to infection, pain, swelling, jaw soreness, cysts, and other systemic illnesses.
A dentist will usually take a panoramic x-ray to view the wisdom teeth to determine when/if they need to be extracted. If extractions are indicated, getting them out at a younger age is preferable for recovery time. If wisdom teeth are fully erupted and not impacted in the gums or bone, they can be extracted as easily as a regular tooth. If they are impacted, the gums and/or bone need to be cut open in order to extract. Wisdom teeth can be extracted with local anesthetic only, though other pain/anxiety management techniques can be used. These include nitrous oxide, conscious sedation, and/or anti-anxiety pre-medication.
Recovering time is usually about 2 weeks. During the recover a person may experience bleeding and facial swelling. Pain medications and antibiotics are often prescribed as well. Possible complications of wisdom teeth extractions include dry socket and parasthesia. Dry socket occurs when a blood clot fails to form or falls out and is extremely painful. A person should not drink from a straw for several days after the extractions to avoid dry socket. During wisdom teeth extractions the nerves can sometimes become damaged or bruised. This results in prolonged numbness or parasthesia, which can last weeks or months and can sometimes be permanent. Parasthesia is more rare and generally the numbing sensation wears off after several hours. Dry socket and/or parasthesia don't always occur after wisdom teeth are extracted.
So you can see that wisdom teeth can cause many headaches (literally)! Be wise and get your wisdom teeth extracted as soon as your dentist recommends it.


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US supports all efforts of talks between India, Pak: State Dept.
Washington, Aug. 23 : The U.S. State Department has said it strongly supports all efforts between India and Pakistan that can contribute to a more stable and prosperous region, adding it includes meetings at any level between the arch-rivals.
U.S. State Department Deputy Spokesperson Mark C. Toner made this remark when asked if Washington would come forward as a mediator on stalled talks between New Delhi and Islamabad.
"Well, first of all, we strongly support all efforts between India and Pakistan to - that can contribute to a more stable and prosperous region, and that includes meetings at any level between Indian and Pakistani officials," Toner said.
He said that Washington would be encouraged if India and Pakistan continue to engage in direct dialogue aimed at reducing tensions.
"Our longstanding position has always been that India and Pakistan stand to benefit from a normalization of relations and practical cooperation, and so we would be encouraged that India and Pakistan continue to engage in direct dialogue aimed at reducing tensions," he added.
Last week, India rejected Pakistan's proposal for Foreign Secretary-level talks on Kashmir and said that terror remains the core concern and it would like to discuss aspects related to cross-border terrorism which are central to the current situation in Jammu and Kashmir.
Pakistan's invitation came after Indian Home Minister Rajnath Singh told the Parliament that New Delhi was willing to discuss the issue of Pakistan-occupied-Kashmir and that the question of discussing Jammu and Kashmir doesn't arise.
← YuppTV to host ScoopWhoop's exclusive viral content on YuppTV Bazaar Ghulam Nabi Azad calls for dialogue to end Kashmir unrest →


This image of connect the dots straight lines is really cute connect the dot pages for your son.
You are enable to download free by clicking the DOWNLOAD button under the picture. It will give access to a new window. Do right click on the snapshot, then choose save picture as… After that you have authorization to changed the file name and the folder where you want to save the file. Then click the save button.
Check out these pictures linked to connect the dots straight lines, hope you like it..


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Agenda for Plenary on Wednesday, 8 June 2022, 13.30
Plenary - Wednesday, 8 June 2022 13.30
Votes Summary PDF 143 KB
Questions to the Minister for Social Justice
The Presiding Officer will call Party Spokespeople to ask questions without notice after Question 2.
The first 8 questions were asked. Question 6 was answered by the Deputy Minister for Social Partnership. The Presiding Officer invited party spokespeople to ask questions to the Minister after question 2.
Questions to the Counsel General and Minister for the Constitution
Questions 1-4 and 6-8 were asked. Question 5 was withdrawn. The Presiding Officer invited party spokespeople to ask questions to the Counsel General after question 2.
At 14.30, proceedings were suspended for 29 minutes. The bell was rung two minutes before reconvening.
Questions to the Senedd Commission
All 5 questions were asked. Questions 1 and 3 were grouped together for answer.
Topical Questions
To ask the Minister for Climate Change
Rhys ab Owen (South Wales Central): What steps is the Welsh Government taking to protect renters following the deferral of implementation of the Renting Homes (Wales) Act 2016?
To ask the Minister for Health and Social Services
Jayne Bryant (Newport West): Will the Minister provide an update on the news that the group that owns the Rutherford Cancer Centre in Newport is to go into liquidation?
Answered by the Minister for Climate Change
Answered by the Minister for Health and Social Services
90 Second Statements
Jenny Rathbone made a statement on - 40th Anniversary of the Sir Galahad/Bluff Cove air attacks.
Samuel Kurtz made a statement on - Celebration of Welsh Food and Farming
Motion to elect a Member to a committee
NDM8021 Elin Jones (Ceredigion)
To propose that the Senedd, in accordance with Standing Order 17.14:
1. Elects James Evans (Welsh Conservatives) as a member of the Legislation, Justice and Constitution Committee in place of Peter Fox (Welsh Conservatives).
(120 mins)
Debate on the report of the Special Purpose Committee on Senedd Reform - Reforming our Senedd: A stronger voice for the people of Wales
NDM8014 Huw Irranca-Davies (Ogmore)
1. Notes the report of the Special Purpose Committee on Senedd Reform, 'Reforming our Senedd: A stronger voice for the people of Wales'.
2. Endorses the recommendations for policy instructions for legislation on Senedd Reform in time for the next Senedd elections in 2026.
3. Notes that the report also calls on the Senedd to consider a number of matters relating to Senedd reform.
Amendment 1 Darren Millar (Clwyd West)
Delete point 2 and replace with:
Regrets the position statement on Senedd reform by the Welsh Government on 10 May 2022 which undermined the independence and work of the committee.
Add as new point after point 2 and renumber accordingly:
Regrets that the voting system proposed by the committee would not permit voters to vote for an individual candidate of their choice.
A vote was taken on the motion without amendment
The motion without amendment was agreed.
Welsh Conservatives Debate - Betsi Cadwaladr University Health Board
NDM8015 Darren Millar (Clwyd West)
1. Regrets that the Welsh Government has failed to deliver promised improvements at Betsi Cadwaladr University Health Board.
2. Believes that the decision to move the health board from special measures in November 2020 was inappropriate.
3. Calls upon the Welsh Government to impose a reformed special measures regime to provide the health board with the leadership and resources necessary to address failings, and deliver the high-quality health care that the people of North Wales deserve.
The following amendment was tabled:
Amendment 1 Sian Gwenllian (Arfon)
Add as new point at end of motion:
Calls for an independent review to be commissioned to consider the possible benefits of replacing Betsi Cadwaladr University Health Board with new structures to deliver healthcare in the north of Wales, due to its chronic problems.
A vote was taken on the motion without amendment:
The motion without amendment was not agreed.
As required by Standing Order 6.20 the Deputy Presiding Officer exercised his casting vote by voting against the amendment. Therefore, amendment 1 was not agreed. As the Senedd did not agree the motion without amendment, and did not agree the amendment tabled to the motion, the motion was therefore not agreed.
Short Debate
NDM8011 Joel James (South Wales Central)
Hidden issues affecting deaf people and those suffering hearing loss


Content I created as the Associate Projection Designer for A Midsummer Night's Dream directed by Julie Taymor for the inaugural production for Theater for a New Audience in Brooklyn in 2013.
Below is Puck's animal transitions which appeared in "shadow" magically mimicking the actor's movements. Audio in this clip is from a rehearsal recording with the text spoken live in performance by Kathryn Hunter who played Puck.
To the right is the purple flower I animated to bloom open, flutter and burst into purple color. To the right also is the tree from which Puck emerges to create the trunk.


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Mr. Hammond: The hon. Gentleman has replied to the intervention of my hon. Friend the Member for Castle Point (Bob Spink) by talking about a postal ballot on an offer that is not on the table. What is his reaction to the suggestion of a postal ballot of all FBU members on the offer that is on the table?
John McDonnell: Time has moved on, and at this point there would, I think, be an overwhelming rejection. As the Deputy Prime Minister said, we can only provide advice according to our individual judgments; that is the best way forward. The atmosphere is so soured that unless the offer is realistic and based on the process that Burchill has gone through, it stands no chance of success—it is as simple as that. We have been led into a quagmire. Rancour has broken out in this dispute because of a series of inept and—I repeat—dangerous interventions.
The FBU executive meets next week. I hope that we can find a way forward, but if the Government think that this legislation can be used as a threat, they need to reassess the people with whom they are dealing. As has been said time and again, firefighters are some of the most courageous members of our community; they put their lives at risk for us every day. They are disciplined, with many coming from a military background. They do not go on strike lightly, and they will not be intimidated easily. They are seriously dedicated people, yet today I heard them be traduced.
I have toured fire stations that are trying to operate the new dimension procedures to tackle ricin and biological warfare. They have been demanding the necessary equipment for nearly 18 months. Some of the equipment that they have been training with during the past six months has been of an appallingly bad standard. We are told today that there are boycotts, but I did not experience a single one in any of the areas that I visited—far from it. Firefighters are willing, yet again, to risk their lives on our behalf. Trying to push through the legislation with threats such as this is totally counter-productive; if anything, it will exacerbate the situation. It will make a settlement more difficult to achieve, not less.
For the Government, this has much wider ramifications. Such coercion of public service workers results in demoralisation. Coercion of one group of trade unions makes others militant and angry, because they know that they could be next. From the Labour point of view, it destroys any semblance of the link between new Labour policies and the tradition of a party and a movement that was founded by the trade union movement, which many trade unionists have devoted their lives to supporting.
This new Labour legislation marks an historic break between the party of Hardie, Attlee and John Smith, and new Labour. If it is passed, do not mention solidarity
8 May 2003 : Column 898
again; do not refer to "our movement"; do not expect the support of firefighters across the country and the many trade unionists who are behind them—and be prepared to go further. Many firefighters will not accept an imposed settlement. What happens then? It was made clear in today's House of Commons briefing that this legislation does contain the power to go further and ban industrial action. So in voting for this legislation, be prepared to go further; be prepared for the consequences: banning strikes and sequestering union funds, taking us back to the Shrewsbury picket days of the imprisonment of trade unionists.
This legislation is a disaster—I never expected a Labour Government to introduce such legislation. It is a disgrace to our movement, and it should be rejected tonight.
Mr. Desmond Swayne (New Forest, West): The principal argument advanced by the Deputy Prime Minister in introducing the Bill was about public safety. He made it clear that, in view of the special delegate conference's rejection of the latest offer and the reduction from 19,000 to 9,000 in the number of troops available to provide cover in the event of continuing strikes, which would have a significant effect on response times, the consequences for public safety could not be tolerated, so he had to impose a settlement. However, I believe that the Bill will not deliver the public safety that the Deputy Prime Minister rightly desires.
As the hon. Member for Hayes and Harlington (John McDonnell) so eloquently pointed out, an imposed settlement will not necessarily be accepted. The Bill has no evident power—unless clause 1(1)(a) contains sinister hidden powers—to ensure that firemen remain at work, thereby safeguarding public safety. The Bill simply does not possess the teeth required to deliver its principal aim of obliging firemen to go back to work.
When the Deputy Prime Minister was challenged when he made his statements to the House at the end of last year and earlier this year, particularly on public safety and whether he would use existing powers to prevent strike action, he consistently said that that was a matter for the Attorney-General and the courts—he had no power. I suggest that the Bill provides an opportunity for him to acquire the necessary power and I hope that the Committee will concentrate on giving him such power, should he so require it. However, giving him the power to impose a settlement raises the question of what exactly he intends to impose. In view of the huge powers that the Bill will accord him to determine terms and conditions and remuneration, he could at least have shared with us—or given us some idea of—what he had in mind in that respect. He has not done so. Will he seek to impose the terms and conditions and the settlement that have just been rejected by the special delegate conference? If not, what terms and conditions will he impose that will provide any chance of bringing about the settlement that he desires?
Will the Minister for Local Government and the Regions explain precisely what is meant by clause 1(5)(a), when it says
"to make provision with retrospective effect fixing or modifying the pay or allowances of fire brigade members (including provision having effect from a time before the passing of this Act)"?
I am not aware of any precedent for such a power, but I would welcome some clarification of what that means.
Mr. Hammond: I thought that I heard the Deputy Prime Minister clearly indicating, by way of threat, that he could not give an assurance that an imposed settlement would be as generous as the one already offered. Did my hon. Friend not hear that? If he is in any doubt, he could ask the Minister to clarify the matter.
Mr. Swayne: I certainly heard that, but I was hoping for some exposition of precisely what the Deputy Prime Minister intended to impose. I hope that, if the Minister will not intervene now, he will favour us with an explanation in his winding-up speech. The fundamental question remains: if the Deputy Prime Minister intends to impose a settlement, why did it not occur to him to seek to negotiate one earlier? There has been a great deal of confusion about the level of negotiation that directly involved the Deputy Prime Minister. We have heard from the hon. Members for Halifax (Mrs. Mahon) and for Hayes and Harlington (John McDonnell) about the extent of the Deputy Prime Minister's involvement, and they described how unhelpful it was. Indeed, when he was at his most passionate and eloquent this afternoon, the Deputy Prime Minister said that he was at the heart of the negotiations. I hope that the record will show tomorrow that that was what he said. I am not sure what the Deputy Prime Minister meant by those words but it is clear that he was exercising a veto throughout the negotiations.
My hon. Friend the Member for Uxbridge (Mr. Randall) pointed out that the Deputy Prime Minister's speech was one of the best that he had made, and I agree. He was eloquent and passionate—most of all when he described his frustration at what he called the misconceptions, uncertainties and lack of dialogue. So why did he not do what the Opposition were urging all along and take a direct, formal part in the negotiations, thereby ensuring that there were no misconceptions or lack of communication?
The Deputy Prime Minister was eloquent when he criticised the employers' communication skills. The negotiations would have benefited from the communication skills that he evidenced this afternoon, when he presented the Bill to the House. The Government should have intervened in the matter when the negotiations were under way, as I pointed out in an intervention. The Deputy Prime Minister did not answer me, saying simply that that was a matter of opinion. We would not be in this position now if there had been direct negotiations, as we argued all along.
One way to get out of the position in which we now find ourselves, short of an attempt to impose a settlement, would be to do what my right hon. Friend the Member for Haltemprice and Howden (David Davis) proposed, and hold a ballot. In that way, we could at least test the water.
The hon. Member for Stroud (Mr. Drew) responded to the proposal for a ballot with the extraordinary argument that no one could be expected to respond in a ballot to a programme of actions or conditions imposed from the centre, when that programme would have a differential effect in different parts of the kingdom. That seems a pretty good argument against ever holding a general election, but I shall let that ride. In Committee, the Opposition would do well to concentrate on the possibility of inserting into the Bill arrangements for a secret ballot of the entire FBU membership.
I shall finish with some questions that I hope the Minister of State will address when he winds up the debate. When will the White Paper on which much of the Bill is predicated be published? In respect of what is being proposed for the future of the fire services, will he say whether there is any substance to the reports on the front page of today's edition of The Daily Telegraph to the effect that the Deputy Prime Minister is urging local authorities to ensure that they charge when the fire service is called out to deal with domestic floods, car crashes, and so on?
What guidelines have been issued for the purposes of drawing up the risk assessments, management plans and consultations that will be instituted once section 19 of the 1947 Act is repealed? I believe that those assessments are being made even now, so I should be grateful to know what guidelines the Government have issued.
In his statement to the House on 28 January, the Deputy Prime Minister said that it cost £1 million a day to provide cover for striking firemen. Will the Minister give some indication as to how that figure is computed, and what it is made up of? Does it include the salaries of the servicemen who provided the cover? That would surely not be a marginal cost, at any rate, as those salaries would have to be paid anyway.
When the Deputy Prime Minister was asked again and again why he had not made the assets and facilities available, he advanced two arguments. The first was that they were not necessary and that the servicemen were not capable of using them. The second, and more important, was that that would only inflame the situation. Perhaps he can explain why these provisions are in the Bill. If they were so unnecessary and unhelpful then, what has changed?
Principally, the Bill simply does not and will not do what the Government hope it will. If it is to do that, it will require substantial amendment in Committee.


Here you'll find quarterly and monthly real estate activity in chart form for homes sold in Ventura, Oxnard and Camarillo and also the data particulars for each city. All data is taken from the Ventura MLS, Oxnard MLS and Camarillo MLS.
NOTE: While the following sold prices and numbers sold reveal some change – or no change, it's important to see at the end the Detailed statistics for pendings (in escrow) for true trends indicating where the housing market is going – not where it's been!!
The monthly chart shows sold prices are softer year over year for all three cities while comparing January of 2011 with December 2010 – Ventura remains unchanged and Camarillo and Oxnard have softened.
Sold prices for condos in the three cities remains practically unchanged year over year and for January compared to the previous month of December.
Year over year shows an increase for Camarillo while Ventura and Oxnard remain steady. Comparing one month of activity shows the number of homes sold in all three cities is softer for January as to December.
Our January newsletter – is our Ventura County housing market like the rest of the country?
In our January newsletter, I said the media tends to paint all the housing markets across the country with one brush stroke. But Ventura County doesn't fit areas like the Inland Empire or the Stockton area with severe falling home prices and numerous foreclosures. Yes, we've been impacted to some degree, but see for yourself our data tracking for 2010. The newsletter has 2 important charts showing quarterly trends in sales and prices, but you can see those trend charts directly here for sales numbers and here for sold prices.
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Karin Schwartz, Ph.D.
Scientific Coordinator for Ex-Situ Partners
Dr. Karin R. Schwartz is a conservation biologist with over 20 years of experience in data management processes as applied to conservation within zoological institutions as well as for field research for endangered species. She holds a M.Sc. in animal behavior from the University of Missouri – St. Louis where she studied individual recognition through olfactory communication in prairie voles. She recently completed a Ph.D. in conservation biology at George Mason University where she worked on research to link in situ and ex situ data management processes for endangered species recovery programs through the use of Species360 Zoological Information Management System. As Registrar at the Milwaukee County Zoo, she initiated and led the development of Association of Zoos and Aquariums (AZA) records-training programs for use of Species360 software and continues to act as co-administrator of the Institutional Records-Keeping Course which is taught as part of the AZA Professional Training Schools. She has taken this course on the road for capacity building to other zoo regions, holding sessions for the 10th Congress of Asociación Latinoamericana de Parques Zoológicos, Acuarios y Afínes (ALPZA) in Buenos Aires, Argentina, South Asian Zoo Association for Regional Cooperation for their SAZARC/RSG meeting in Coimbatore, India and the Korean Association of Zoos and Aquariums in Seoul, S. Korea.
Within the AZA community, Karin has extensive experience with population management and records-keeping as well as serving on Animal Data Information Systems Committee, Field Conservation Committee and Institutional Data Management Advisory Group. She has been involved in an advisory capacity to AZA population management programs as Registrar Advisor for the Tapir Taxon Advisory Group (TAG), Monotreme and Marsupial TAG, Cheetah Species Survival Plan® (SSP®), and Matschie's Tree Kangaroo SSP®. In working at zoological institutions, she realized the necessity of collaboration with and participation in IUCN/Species Survival Commission Specialist Groups for effective conservation action. As Registrar Advisor for the AZA Tapir TAG, she became involved with the Tapir Specialist Group which effectively integrates in situ and ex situ specialists into holistic conservation action for tapirs. She contributed to conservation action planning through work on the Tapir Specialist Group Strategic Plan 2008 – 2010, on the CBSG team to facilitate the Western Pond Turtle Population and Habitat Viability Assessment, and as a reviewer for the Re-introduction Specialist Group's Guidelines for Re-introductions and Other Translocations (2013). Karin has long been involved with cheetah conservation as a Registrar advisor to the AZA Cheetah SSP® and specifically with the Cheetah Conservation Fund (CCF) through her assistance with the International Cheetah Studbook, involvement in records training for CCF international and Namibian staff, and as an author for a chapter discussing the conservation importance of ex situ cheetah populations for an upcoming book on cheetah conservation and science. Recently, she was the Intern Research Coordinator at CCF in Namibia, overseeing intern duties for cheetah and dog care and records-keeping as well as for intern involvement in CCF's Ecology Department programs.


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Home / Top 10 Places to go in Tokyo / Top 10 Places to go in Tokyo
Top 10 Places to go in Tokyo
The Tokyo Tower is one of the most iconic landmarks in Tokyo and it's without a doubt, one of the top 10 places to go in Tokyo to enjoy memorable views of the city.
Top 10 Tourist Attractions in Japan
The views from the Tokyo Tower are truly amazing, especially at night when Tokyo is beautiful illuminated. Besides enjoying the views from the observation decks, the Tokyo tower is home to a wax museum, an aquarium and the Trick Art Gallery, which boasts really cool 3D pictures and sculptures.
Roppongi District
The district of Roppongi is no doubt, one of the top 10 places to go in Tokyo to enjoy a night out as it's hottest nightlife spot in the city.
Roppongi truly comes alive on the weekend and it's a popular hang-out spot with tourists so you'll find lots of international bars, clubs and restaurants such as Outback Steakhouse, Agave Bar and the Bahaus which is a cool restaurant/ bar where you can enjoy live music.
Shinjuku Gyoen is located in the district of Shinjuku and it's definitely one of the top places to go in Japan to get away from the Tokyo crowds. The Shinjuku Gyoen Garden is lovely and tranquil spot with a beautiful variety of Japanese and European garden areas, as well as grassy areas where you can sit and relax and enjoy a nice picnic.
By the way, if you love cherry blossoms be sure to visit the Shinjuku Gyoen Garden during the cherry blossom season as the garden is one of the best spots in Tokyo for cherry blossom viewing (hanami).
Shibuya District
If you're visiting Tokyo for mainly shopping, you'd want to add the Shibuya district first on your list of top 10 places to go in Tokyo.
Shibuya is one of the most popular and lively shopping areas in Tokyo and the district is filled with lots of fashionable shops and department stores such as Tokyu Hands which is one of the best places to do souvenir shopping in Tokyo. Another popular shopping spot in Shibuya is Disk Union which is a cool music shop where you can buy second-hand CD's and music equipment at bargain prices.
Tokyo Government Building
The Tokyo Government Building is located in Shinjuku and it's another of the top 10 places to go in Tokyo to enjoy panoramic views of the city.
You can get excellent views of downtown Tokyo from the Tokyo Building (even of Mt. Fuji on a clear day), and the best part of a visit to the Tokyo Government Building is that it's free to get in!
Roppongi Hills
Roppongi Hills is without a doubt the cultural hub in Tokyo, and one of the top 10 places to go in Tokyo for a great experience. Roppongi Hills is especially fun to visit during the summer months as the area hosts lots of regular events such as the Bon ODori Festival (July 16th-17th) and the Beer Festival (June 15th-16th).
Roppongi Hills is also home to the Mori Art Museum and Tokyo City View, which is home to a 360 degree observation deck and Aquarium in the Sky where you can experience the feeling of being underwater. There are also a ton of restaurants and shops, as well as cinemas, green areas, and luxury hotels such as the Grand Hyatt Tokyo.
Tsukiji Fish Market
Tsukiji Fish Market has to be one of the world's most amazing fish market, and it's one of the top 10 places to go in Tokyo hands down! If you're a fan of sushi and seafood, you'll love spending time at the Tsukiji Fish Market seeing the huge fish, mingling with locals and soaking in the old traditional Japanese charm.
The fish market is truly huge and can be overwhelming to explore for the first time so it's a good idea to book a guided tour with Tsukiji Fish Market Guided Tours, which will also include a sushi lunch, a chance to learn about fish monging techniques and much more.
Tokyo National Museum is one of the best museums in Japan and one of the top 10 places to go in Tokyo to admire amazing architecture and view interesting artifacts on display.
Beautiful Places in Asia to visit on vacation
The Tokyo National Museum is located in the Ueno district and boasts various areas (all with information and signage in English) such as the Buddhist exhibit section which includes lettering art from the 9th century, a 3D Mandala, and Buddha statues; as well as the grand Heiseikan section, and the Hokan Gallery where you can learn in detail about Japan's history.
See. Top Sites to See in Japan
Sights to See in Yokohama
Tourist attractions in Kyoto Japan
things to do in Okinawa Japan
Places to visit in Taiwan
Top 10 Places To Go In Southeast Asia
Places to see Cherry Blossoms in the US
top 10 places to see in Hong Kong
Tourist Spots in the Philippines
Places in Singapore to visit
10 must see places in Bangkok
Places to visit in Jakarta Indonesia
Great vacation spots in Asia


had an acident 5 years ago,brain damage now am told if I do not win work mens comp I can not go to trail for the main case,is this true?
My employer is based in CA and I work from Florida. Got laid off as of Nov 15.Unemployement benefits must be claim where?


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