On November 21-23 the Walker will present Disabled Theater, a collaboration between Paris-based choreographer Jérôme Bel and ten actors with disabilities from the Zurich-based company Theater HORA. Formed in 1993, Theater HORA took its name from a character in the company's first production. The group has performed in numerous festivals around the world, including all over Europe and in South Korea, and has won multiple awards. Theater HORA members have acted for television and performed in a professional dance video, and one HORA actress, Julia Häusermann, was awarded the Alfred Kerr Acting Prize in Berlin last May.
Jérôme Bel was introduced to Theater HORA in 2010, and initially was not interested in working with the company. Nevertheless, he watched some clips of their work and was deeply moved: "The emotion I felt was so strong that I couldn't think. I realised that I wouldn't be able to understand this emotion, which is unusual for me. My desire to work with them came from this first experience because I needed to understand what had happened to me the first time I saw them." The premise of Disabled Theater is simple: it is a staged re-telling of Bel's first interactions with the theater HORA actors.
While Theater HORA is one-of-a-kind in Switzerland, there are numerous theater companies around the world that include people with disabilities. Last January, Back to Back Theatre, a company based in Australia, came to Minneapolis to perform as part of the Walker's annual Out There series (for more information on their work, check out this conversation between Back to Back artistic director Bruce Gladwin and Walker Web Editor Paul Schmelzer).
Minneapolis is also home to Interact, a center for visual and performing arts whose mission is "to create art that challenges perceptions of disability." Founded in 1996, the organization provides theater and studio art opportunities for more than 125 artists with disabilities.
The day after their arrival in Minneapolis, Theater HORA paid a visit to Interact Center, where they took a tour of the studios, galleries, and rehearsal spaces, and met many of the visual and performing artists. Since Interact actors are busy with their current show and will not be able to see Disabled Theater, Gianni and Remo of Theater HORA gave brief, spirited performances of their dances that they will perform at the Walker this weekend. Interact founder and creative director Jeanne Calvit spoke about her organization and fielded questions from the HORA actors, and Interact actors asked questions about Theater HORA, communicating through translators. To wrap up the visit, everyone joined in an energetic dance party that opened up a new realm of communication to easily circumvent the language barrier.
Calvit also had much relevant insight to share about creating art with people with disabilities. She told me about Interact's collaborative process: the actors and staff create their plays through improvisation, allowing everyone in the company a chance to contribute. She explained the effectiveness of working with actors with disabilities through improvisation: "If we did theater in the more traditional way, giving everybody a script and saying 'this is your role,' it wouldn't have the same passion because they're not invested in it. I think anybody who works through improv is going to have a lot more success with people with disabilities. A lot of them do better when they're thinking on their feet and they can improvise than if you just give them a piece of paper and say 'your role is this, you need to memorize that, and I'll tell you where to move.'" Disabled Theater, in which the actors of Theater HORA play themselves, similarly represents the spirit of honesty and collaboration that underlies the creation of these works.
Marcel Bugiel: Aren't you afraid that some in the audience will think you're staging a freak show, that you're exploiting these actors and exposing their disabilities, that there's an element of voyeurism in the show?
Jérôme Bel: That doesn't worry me. For me theatre is precisely about being able to see what you're not used to seeing, what's hidden and concealed from view… The question of performance by people with learning disabilities is complicated because these days it's highly unthinkable. You don't know how to react when you're confronted with them, their presence is hugely embarrassing because they're not represented in the public domain. And for as long as that is the case, there will continue to be embarrassment and uneasiness. The only method is confrontation… this community has to be given greater visibility.
In its raw, honest fashion, Disabled Theater guarantees greater visibility of people with disabilities by placing them and their life stories on stage, necessitating that audience members confront their own preconceptions and assumptions about disability.
Jérôme Bel and Theater HORA present Disabled Theater November 21-23, 2013 at 8 PM in the McGuire Theater. Stay after the performances for a postshow reception with the artists (Thursday, November 21), a Q&A with Jérôme Bel (Friday, November 22), and a SpeakEasy discussion with local artists and a Walker tour guide (Saturday, November 23).


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Category Archives: Longshore
Longshore Employment Defined.
May 14, 2018 Greg Lois
Coverage under the Longshore and Harbor Workers' Compensation Act requires a master-servant relationship between employer and an employee. Crowell v. Bensen, 285 U.S. 22, 54 (1932). The Act defines employee as "any person engaged in maritime employment, including any longshoreman or other person engaged in longshoring operations, and any harbor-worker including a ship repairman, shipbuilder, and ship-breaker." 33 U.S.C. § 902(3). The Act also sets out a long list of potential employees who do not fit the criteria: clerical and secretarial workers, marina workers, fishermen, etc.
The issue of employment is usually reached int he context of an alleged independent contractor seeking benefits. Just because an employer has not secured LHWCA coverage for an employee does not mean that that worker is not covered under the Act as an employee. Tanis v. Rainbow Skylights, 19 BRBS 153 (DOL Ben. Rev. Bd. 1986). Continue reading Longshore Employment Defined. →
The Jones Act v. Longshore
May 7, 2018 Greg Lois
Which workers' compensation act applies: the Longshore and Harbor Workers' Compensation Act or the Jones Act? The Jones Act and the LHWCA are mutually exclusive. Thus, when dealing with a "water-based" (as opposed to "land-based") LHWCA claim, it must be determined if the claim falls within the criteria of LHWCA coverage, or belongs more properly under the Jones Act. Of course, the claim might not belong under either jurisdiction and should be decided under a state workers' compensation act.
The Jones Act (The Merchant Marine Act, 1920, 46 U.S.C. § 688), in pertinent part, reads as follows:
Any seaman who shall suffer personal injury in the course of his employment may, at his election, maintain an action for damages at law, with the right of trial by jury, ... and in case of the death of any seaman as a result of any such personal injury the personal representative of such seaman may maintain an action for damages at law with the right of trial by jury. ... Jurisdiction in such actions shall be under the court of the district in which the defendant employer resides or in which his principal office is located.
Admiralty jurisdiction and the coverage of the Jones Act depends only on a finding that the injured was "an employee of the vessel, engaged in the course of his employment" at the time of his injury. The fact that a Jones Act petitioner's injury occurred on land is not material. 46 U.S.C. § 740; Senko v. La Crosse Dredging Corp., 352 U.S. 370, 373 (1957). Continue reading The Jones Act v. Longshore →
Understanding Jurisdiction Under the Longshore Act
April 30, 2018 Lois LLC
The Longshore and Harbors Workers' Compensation Act ("LHWCA") covers longshore/harbor workers and other maritime workers. The Act has also been applied to certain other workers under the Defense Base Act.
"Status" and "situs."
The LHWCA set forth the requirements for coverage. "Status" refers to the nature of the work performed; "situs" refers to the place of performance.
The employee claiming benefits under the LHWCA must be engaged in maritime employment, including any longshoreman or other person engaged in longshoring operations, including any harbor-worker including a ship repairman, shipbuilder, and ship-breaker. There are specific exclusion which apply to status (see below). Continue reading Understanding Jurisdiction Under the Longshore Act →
33 U.S.C. § 903(a)Situs
Overview of Longshore Benefits
April 26, 2018 Greg Lois
The Longshore and Harbor Workers' Compensation Act provides for medical benefits and disability benefits to an injured worker. An injured employee is entitled to reasonable and necessary medical, surgical, and hospital treatment and other medical supplies and services required by the work-related injury or illness, such as prescription medications, diagnostic tests, physical therapy, prostheses, hearing aids, attendant care, and the cost of travel for such treatment. An injured employee is entitled to select a physician of his/her choice to provide medical treatment for the work injury.
The LHWCA provides for the payment of compensation for the following four types of disability: temporary partial, temporary total, permanent partial, and permanent total. This compensation can not exceed two-thirds of the employee's average weekly wage during the period of disability, subject to maximums and minimums. 33 U.S.C. § 908 Continue reading Overview of Longshore Benefits →
33 U.S.C. § 908
Defenses, LHWCA, Longshore
The Statute of Limitations in Longshore Claims
The right to compensation for disability or death is barred unless a claim therefore is filed within one year after the injury or death. 33 U.S.C. § 913. Continue reading The Statute of Limitations in Longshore Claims →
Defenses, Longshore
Notice as a Defense in Longshore Claims
It is the claimant's burden to establish timely notice. Notice of an injury or death for which compensation is payable must be given within 30 days after injury or death, or within 30 days after the employee or beneficiary is aware of, or in the exercise of reasonable diligence or by reason of medical advice should have been aware of, a relationship between the injury or death and the employment. 33 U.S.C. § 912(a). The claimant is provided a presumption that timely notice has been provided. Shaller v. Cramp Shipbuilding & Dry Dock Co., 23 BRBS 140 (1989). Where one injury arises out of an accident has been reported, the claimant does not have to give separate notice of other injuries resulting from the same incident. Thompson v. Lockheed Shipbuilding & Constr. Co., 21 BRBS 94 (1988). Continue reading Notice as a Defense in Longshore Claims →
33 U.S.C. § 912(a)33 U.S.C. § 912(c)Lewis v. Todd Pacific Shipyards Corp.Morin v. Bath Iron Works Corp.Shaller v. Cramp Shipbuilding & Dry Dock Co.Thompson v. Lockheed Shipbuilding & Constr. Co.
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Comparing the Risk Profiles of Renewable and Natural Gas Electricity Contracts: A Summary of the California Department of Water Resources Contracts
Devra Bachrach, Ryan H Wiser, Mark Bolinger, William H Golove
LBNL Report Number
LBNL-50965
Electricity markets in the United States have witnessed unprecedented instability over the last few years, with substantial volatility in wholesale market prices, significant financial distress among major industry organizations, and unprecedented legal, regulatory and legislative activity. These events demonstrate the considerable risks that exist in the electricity industry. Recent industry instability also illustrates the need for thoughtful resource planning to balance the cost, reliability, and risk of the electricity supplied to end-use customers. In balancing different supply options, utilities, regulators, and other resource planners must consider the unique risk profiles of each generating source. This paper evaluates the relative risk profiles of renewable and natural gas generating plants. The risks that exist in the electricity industry depend in part on the technologies that are used to generate electricity. Natural gas has become the fuel of choice for new power plant additions in the United States. To some, this emphasis on a single fuel source signals the potential for increased risk. Renewable generation sources, on the other hand, are frequently cited as a potent source of socially beneficial risk reduction relative to natural gas-fired generation. Renewable generation is not risk free, however, and also imposes certain costs on the electricity sector. This paper specifically compares the allocation and mitigation of risks in long-term natural gas fired electricity contracts with the allocation and mitigation of these same risks in long-term renewable energy contracts. This comparison highlights some of the key differences between renewable and natural gas generation that decision makers should consider when making electricity investment and contracting decisions. Our assessment is relevant in both regulated and restructured markets. In still-regulated markets, the audience for this report clearly includes regulators and the utilities they regulate. In restructured markets, the role of regulatory oversight of resource planning is more limited. Nonetheless, even in restructured markets, it is increasingly recognized that regulators have a critical role to play in directing the resource planning of providers of last resort – electric suppliers that provide service to those customers who choose not to switch to a competitive supplier. Our review of electricity contracts may also have educational value for those unfamiliar with the typical contents of these agreements. Details of our findings are provided in the body of the paper, but this summary is written to provide a concise alternative to reading the full report.
LBNL
electricity markets and policy group, energy analysis and environmental impacts department
Refereed Designation
Report PDF (605.29 KB) Appendix PDF (459.26 KB)
Electricity Markets and Policy Group, Energy Analysis and Environmental Impacts Division
Electricity Markets and Policy, Renewable Energy, Cost, Benefit, & Market Analysis


The HM Treasury issued British Pound banknotes in 6 different denominations, including this HM Treasury Ten Shillings banknote (King George V red white). They are part of the withdrawn HM treasury banknotes series. The HM Treasury started issuing these 0.5 British Pound banknotes in 1914. They were withdrawn from circulation in 1920.
The banknote of ten shillings, half a pound, portrays King George V. The words 'United Kingdom of Great Britain and Ireland' are printed on the note.
Repeat these steps for any other notes and coins you want to exchange. Complete checkout and get paid within 5 days of receiving your currencies. To exchange your HM Treasury Ten Shillings banknote (King George V red white) for cash: add it to your wallet now!


Style: Lot / Land "50 acres"
Seller Financing Available! 90 acres with great westerly views! Very private, near recreational trails, power nearby. Perfect location for a cabin or homesteading. It has a few small field areas for some gardens, animals and more.


..and also where there isn't!
Stephen Lawson, Partner and Head of Litigation will be speaking at the next Warrington First event on Wednesday 15th February 2017 at the Institution Bar, 2 Palmyra Square, Warrington WA1 1B.
Stephen will be discussing the pitfalls of having a Will, and not having a will. An issue that is very topical and relevant to us all.
The event starts at 12.45 with registration and networking, lunch will be served at 1.00pm and Stephen will speak at 1.30pm Registration is at 12.45, with the usually friendly and informal networking – lunch will be served at 1pm with Stephen's presentation at 1.30. The cost to attend is £10 per person, pre-booking is recommended as places are limited.
To book your place contact Jennifer McGuinness jennifer.mcguinness@fdrlaw.co.uk, please note that payment is required at time of booking.


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Mohammad Asif (born 11 November 1973) is a Pakistani cricket umpire. He has stood in domestic matches in the 2017–18 Quaid-e-Azam Trophy and the 2016–17 Regional One Day Cup. In December 2018, he was one of the on-field umpires for the final of the 2018–19 Quaid-e-Azam Trophy.

References

External links

1973 births
Living people
Pakistani cricket umpires

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DNSimple Blog - Page 49 - Geeking out over domain names and DNS hosting, from domain management automation to remote working.
The domain manage page is your dashboard for everything about your domain. We're happy to introduce a new page that should make everything easier.


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Xiaomi Mi TV 4S 43 : What is it worth?
What is the new Xiaomi Mi TV 4S 43 worth ?
In addition this Xiaomi displays a Ultra HD 4K resolution.
Mi TV 4S 43 from Xiaomi – Design
Nothing has changed in the design of this new model. Xiaomi continues to ride the wave of its design to which we are now accustomed.
This new Xiaomi Mi TV 4S 43 adopts a standard shape with a flat screen.
With an interesting size of 43 inches, Another thing we can highlight in the design of this TV is the design of its support. Indeed it has a double base, in which we have a division where each part of the base has its place. With this, the TV is exactly the same on both sides, thus creating a very symmetrical and stylish TV that can be enjoyed in any room.
The interest of the Mi TV 4S 43 is undoubtedly its technology Slim design.
TV screen presentation Xiaomi Mi TV 4S 43
With this new Mi TV 4S 43 Xiaomi, the company makes an assumed technological choice. The use of LEDs provides an excellent image.
The Xiaomi television set displays a Ultra HD 4K resolution. In the end, it is interesting to note that beyond the size, the screen is well made. A point to add when choosing between this model and other competitors.
Mi TV 4S 43 from Xiaomi – The sound
The television set is equipped with two speakers with 6 W at output.
Mi TV 4S 43 from Xiaomi – Embedded intelligence
On the software side, the Xiaomi Mi TV 4S 43 is equipped with the operating system Android 9 installed; it allows you to explore a whole universe of entertainment.
You will also be able to access the Internet, either through your Wi-Fi connection, or for the most geek among you via the LAN; you will then be able to download a whole bunch of applications and movies.
This software environment innovates on the Xiaomi Mi TV 4S 43 with a new feature: control the television set with voice. Indeed, thanks to the voice control function, it is possible to change channels, TV sources, turn the TV volume up or down just by talking to it. Isn't that convenient?
Presentation of the technical features of the Xiaomi Mi TV 4S 43
Here is a summary chart of the technical features of the TV:
Sizes of the television without stand 96,1 Zentimeter x 60,8 Zentimeter x 9,8 Zentimeter
Sound power 6
Smart TV Android 9
Component Input (YpbPr) Yes
To conclude on the Xiaomi Mi TV 4S 43
In addition, it is worth noting that this Mi TV 4S 43 is equipped with smart TV technology. Thanks to this, you have a top-of-the-range device in its category, which makes it a relevant choice.
With its Direct LED panel, this TV is doing better than average. The lighting is uniform.


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You are here: Home / Food / 11-Yr-Old Grace Callwood, "We Cancerve Movement" Founder, Named Harford's Most Beautiful Person
11-Yr-Old Grace Callwood, "We Cancerve Movement" Founder, Named Harford's Most Beautiful Person
October 12, 2015 By Dagger News Service
From We Cancerve Movement:
Grace E. G. Callwood, 11, was named Harford's Most Beautiful Person during a ceremony at the Bel Air Church of the Nazarene. She was the only nominee under the age 18 in this year's competition.
Callwood, who founded the We Cancerve Movement in 2011, was among the 22 individuals and four couples nominated for the top prize this year.
Each year, the Harford County Department of Community Services searches for local citizens who selflessly assist those around them, and honors their service at its annual Harford's Most Beautiful People Awards Ceremony. According to the county's website, the award nominees are volunteers of any age or background living anywhere in Harford County, with one thing in common: They have enriched the lives of our citizens through their acts of kindness and concern.
Callwood was nominated by Harford Family House's Director of Operations Mary Barry, and by longtime community advocate and educator Claudia Perry Sconion.
"I was so happy and excited that I won. I was really unexpecting [stet] this and it was just amazing. I'm just thankful to my board, my parents and I'm really proud of all of the other nominees as well," said Callwood.
Since age 2, Callwood has actively supported community initiatives. In 2006, she asked her family to donate a red wagon to the Upper Chesapeake Medical Center's pediatric unit, after a brief hospitalization. There, she enjoyed riding the wagon throughout the halls of the hospital and wanted to make sure the hospital had two wagons to prevent other children from waiting for their turn at fun while hospitalized. In 2007, she asked for toys and games in lieu of gifts for her birthday, and donated them to that hospital.
In 2008, she joined her family in Louisiana in the spring and donated non-perishable food items to a Hurricane Katrina relief organization. Later that year, for her birthday, she requested non-perishable food items as presents when she learned from a local news station that the Maryland Food Bank's supplies were running low. She donated several boxes of food to the food bank.
In 2013, Callwood named an all-youth board of advisors. Under her leadership, We Cancerve has donated more than $10,000 in cash grants and products, including more than $1,100 cash to Sinai Hospital and Casey Cares Foundation.
To date, We Cancerve has donated 318 Easter baskets and 210 new toys and gifts to homeless, sick and foster children; 140 back-to-school outfits to every child at a local homeless shelter for the past three years; 65 winter coats for homeless children and 150 TransFUNers kits and 41 Beach in a Bucket kits to pediatric patients. We Cancerve hosted several events for teen foster girls including a beauty night, movie and pizza night and a line dance party. In 2015, We Cancerve created and sponsored Camp Happy, a summer enrichment program created specifically for children living at Anna's House, a homeless housing program for women and children in Bel Air.
Camp Happy II will launch in summer 2016 at Harford Family House in Aberdeen.
"I think that everybody that was there does amazing things. I think it's so great that there so many people in the world who do amazing things like that," said Sarah Beall, 10, who sits on the We Cancerve Movement's Board of Advisors.
"People may think that what we do is great but they are giving me more. I think it's awesome we get to help them and it's an honor," said Beall.
For October and November, We Cancerve is focusing its efforts on raising awareness of homeless youth.
Milkin' It
We Cancerve has partnered with William S. James Elementary School on a month-long loose change fundraiser to purchase milk for homeless children at Eastside Family Emergency Center in Baltimore County, and homeless and less fortunate children and families who dine at the Sharing Table in Edgewood. The collection began October 2 and will conclude November 2.
October 16 from 9 a.m. to 1 p.m. – Hookup a Home @ Harford Family House
The We Cancerve Movement is going to prepare an apartment at the Harford Family House properties so that it's ready for a family to move in. Join us in the community service project! Those interested in volunteering should email peoplewhocare@wecancerve.org.
October 23 from 7 p.m. to October 24 at 7 a.m. – Homeless for a Night
Sign up today at www.wecancerve.org to sleep in outdoors or in the scariest place in your house to place yourself in a homeless-like situation. This event will raise awareness of the plight of homeless youth, and raise money to support programs designed for them at three shelters: Anna's House, Harford Family House and Eastside Family Emergency Shelter.
November 15 from 1 p.m. to 3 p.m. – Toiletries Packing Party
Let's grab dinner at Broom's Bloom Dairy in Abingdon then pack toiletry kits that will be delivered to homeless families in Harford County. RSVP today at www.wecancerve.org.
We Cancerve has established an accumulator fund with the Community Foundation of Harford County (CFHC). Contributions made to the Community Foundation of Harford County are tax-deductible, and can be designated to the We Cancerve Accumulator Fund. For more information, visit www.wecancerve.org
About Dagger News Service
Congratulations on a job well done!
Hilary says
Lovely! The world needs more people like you…..
Mommy Dearest says
What an inspirational, amazing, kind-hearted young lady! I'm sure many more good things are headed your way.


A plethora of Latin leaders and professionals gathered in the beautiful hill country of San Antonio, Thursday, September 21st for the third annual Latino Leadership Conference hosted by the Council for Latino Workplace Equity. The umbrella topic for the day was "Latinos in the US: Retos y Liderazgo" (challenges in leadership), attendees had a full day with an agenda that included multiple concurrent session covering Latinos in the workplace, a luncheon keynote given by Colorado Speaker of the House, Crisanta Duran and an awards ceremony that honored 14 top Latinos in leadership.
The conference rooms were buzzing with professionals as they poured in for registration at 8:30 am while grabbing complimentary breakfast bites and hot coffee. There was no time wasted as the first concurrent sessions kicked off at 9 am sharp. Attendees could choose between a discussion about Latinas in politics and diversity and change for Latino(a)s in the United States. Other sessions throughout the day included; LGBT Latinos: Impact, Acceptance and Progress, Latino Diversity in the Executive Board and C- Suite and The Importance and Value of Hispanic/Latino ERG'S in The Workplace.
As people moved from session to session, there was plenty of conversations from the panel speakers revisiting the topics even outside of the session. "It was really interesting speaking in the LGBT panel discussion because although I've been apart of this community for as long as I can remember, it never played a role in my professional life. And a lot of time I try to do my job and be me without bringing in the fact that I'm a woman, I'm Latina and I'm gay. I just wanted to be the best at what I do," said Dr. Rachel Yvonne Cruz, of the International Academy of Music and the Arts. "But I realize now through conferences like these that certain issues need to be addressed for specific communities," Cruz continued.
Networking chatter filled the air as everyone got ready to head to the ballroom for the awards and keynote luncheon. It was a packed house with an array of emotions from laughter to tears. National Diversity Council CEO, Angeles Valenciano, gave a heartwarming, tear jerking speech about her friend and Legacy Award Winner, the late Jaime P. Martinez, Founder and Chairman of the Board Cesar E. Chavez Legacy & Educational Foundation. His family accepted the award on his behalf.
The 14 Top Latinos In Leadership all came up to receive their award while the attendees got to know a little more about them. Among the people recognized was Houston Police Chief, Art Acevedo; US Congressman, Joaquin Castro; Director of Counsel at Target, Mara Garcia Kaplan;Texas Supreme Court Justice, Eva Guzman and Executive Director, Global Corporate Payments at American Express, Carlos Tapia.
As everyone settled down we also heard from a DREAMER, Ximena Mondragon, a political science student at St. Mary's University. She took on the role of introducing the keynote, Crisanta Duran, and as she stated, "one of her biggest role models, and someone she hopes to be like someday," and help set an inspirational tone. A message about encouraging people to use their full potential from Ms. Duran was one of the highlights of the conference to some of the attendees. "This is my first year attending the conference and I really enjoyed all the tips that were given during the keynote about what to do to make more of an impact on our Latin community," said attendee, Caroline Tucker.
After filling up on good cuisine and noteworthy words, attendees headed back to the conference rooms to continue with the concurrent sessions and wrap up the day. Guests, speakers and awardees enjoyed a full day of fellowship, learning and celebrating the Latino community.


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HomeFeaturesFeaturesAlan Ashby talks 'American Dream' tour
Alan Ashby talks 'American Dream' tour
Guitarist of Of Mice & Men discusses touring, influences before the band's March 2 show.
24 February 2014 Paige Gross Features, Music
Alan Ashby is blasting music and head-banging backstage.
He throws back a beer and periodically puts the drink down for some lunges and squats.
He's about to perform.
Ashby, the guitarist of Of Mice & Men, is loyal to his pre-show ritual. The band will open for Bring Me The Horizon on March 2 at the Electric Factory.
Although Ashby is only 22 years old, he said he can feel his body deteriorating slowly every time he walks onstage and performs the band's metal music.
Ashby joined Of Mice & Men three years ago after briefly working at KFC.
"I went from scraping s— off the walls of a fast-food restaurant bathroom to being in a rock band in the same year," Ashby said.
Since joining the band as the rhythm guitarist, Of Mice & Men has played Warped Tour three times, put out two albums and embarked on a few world tours.
The band got its start after the two initial members, vocalist Austin Carlile and bassist Jaxin Hall, posted a cover of the song "Poker Face" by Lady Gaga on its MySpace page in 2009.
"They just wanted people to know that the band existed," Ashby said.
Two months after uploading the video, the page had more than a million hits, as well as a few more members.
In the two years following the cover, members came and went, but by early 2012, Carlile, Aaron Pauley, Phil Manansala, Valentino Arteaga and Ashby made up the current members.
Ashby said he feels fortunate that the band was able to take advantage of social media.
"You kind of are your own press source now," Ashby said.
Ashby said he grew up listening to bands that taught him how to play guitar. By the time he got to high school, he could play three albums on guitar: Guns N' Roses' "Appetite for Destruction," Linkin Park's "Hybrid Theory" and Papa Roach's "Incest."
"I think everybody grows up as a fan of music, learning to play the covers from the people they want to be like," Ashby said.
Ashby was able to play next to some of these musicians while Of Mice & Men played at Warped Tour.
The band name, Of Mice & Men, originates from the classic novel by John Steinbeck, paying homage to the theme of the novel.
"It was a book that every kid had to read in high school, it's about the American Dream," Ashby said. "What is a rock band if it's not the American Dream?"
The band embarks on its "American Dream" tour on Wednesday at the Starland Ballroom in Sayreville, N.J., and will be in Philly on Sunday.
Ashby said he had never been out of the country before joining the band, but since then has traveled the world.
"You wouldn't expect it, but the areas that aren't big cities seem to appreciate music more," Ashby said. "In Scotland, people were doing some crazy s—, jumping out of windows and moshing."
Ashby said the biggest moment for him so far was flying over Japan knowing that he was going to play a show there the next day.
"It was like New York without an end, it just kept going, and knowing that we were playing a city like that was just mind blowing," Ashby said.
Ashby also said hearing the band's music for the first time on Sirius XM Radio in a friend's car was a standout moment in his career.
"My friend was like 'Dude, I knew you were in a band, but I didn't realize you were that big,'" Ashby said.
Of Mice & Men released its third album, "Restoring Force," in January. The record was a five-month process, and while Ashby said there's a lot of worrying behind the scenes, the finished product is well worth it.
"It's really cool to put out something that so many people like and can relate to and are excited about," he said.
"It's like a painting when it's all done," Ashby said. "Looking back and thinking, 'Yep, that's what I wanted it to look like.'"
Paige Gross can be reached at paigegross1@temple.edu.
Paige Gross
Craig: Campus growth will cause issues
Temple Mens Basketball falls to No. 21 UCONN
Thanks for taking the time to write about these guys, Paige! I work with a lot of bands like Of Mice & Men, mostly connecting them with fans, and anytime I've done a festival they're on, they always stick around longer than necessary to spend time with the kids. It's incredible to hear stories from the kids about the insanely rough times (broken homes, depression, etc.) they accredit Of Mice & Men with getting them through, and I've seen the band be nothing but grateful. I know this whole genre isn't a favorite among college kids, but I really appreciate you taking the time to give them some deserved attention. 🙂


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Q: How to invoke a custom code generating target before a VC++ project is compiled with VS 2019? I have a solution with C# and C++ projects. Visual Studio 2019 is what is being used to build. I have a target that generates a .h file which affects all the native projects (i.e. C++ projects).
I like this target be invoked before compiling any C++ code.
How do I do it?
I added a property like below and rebuilt the C++ project. I don't see MyGenFiles getting invoked.
<BeforeClCompileTargets>$(BeforeClCompileTargets);MyGenFiles</BeforeClCompileTargets>
Update
Above is the right approach to get this done. It happened to be my verifications that was the problem. I added a message task as Jonathan suggested, I could see the target getting invoked.

redpajama_set_name: RedPajamaStackExchange


However, if you are placed under arrest and make statements to the police that are not the result of police questioning, then those statements can be used against you regardless of whether you had your rights read to you.
If you are placed under arrest it is very important that you say absolutely nothing under no circumstances, regardless of whether your rights have been read. You should make it very clear to the police that you have nothing to say to them and that you want to speak to an attorney.
If you have been arrested and interrogated with or without having your Miranda rights read to you, and are in need of a criminal defense lawyer in DC, contact Farrelly PC for a full and free consultation.


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The 11 Biggest Cruise Ships in the World
Published on May 18, 2015 at 7:57 am by Vincent Roy in Lists
Let's go even bigger with the 11 biggest cruise ships in the world. Cruise ships have always been one of the more luxurious getaways a man can afford. Spanning from weeks to even months of travel, there's nothing quite like the experience of getting away on a cruise in the open sea. Cruise ships allow one to take a break from normal life and reality as well as give people the opportunity to travel and see the world on a leisure ride.
Cruise ships have of course over time improved, with voyages spanning long periods of time. It was necessary to keep people entertained and satisfied during the long trip. So as time went on more and more amenities became available to cruise ships, with the increase of things to do and people availing of trips. Cruise ships in turn got bigger and bigger, with bigger and grander cruise ships offering what smaller cruise ships cannot. You would be surprised at how big some of these cruise ships can get.
Now if you're looking for something more on the line of shipping, check out our list of The Ocean's 10 Biggest Shipping Companies.
This list is definitely about size, though one thing you'll notice that only three companies are handling all these 11 cruise ships, which says a lot about how dominating these companies are with regards to luxurious cruises. Let's go ahead and check the first ship on this list:
10 Countries with the Longest Coastline in the World 8 Best Places To Visit in Turkey Before You DIe 5 Best Places to Visit in Thailand for Kids and Families 11 Biggest Music Festivals in the World 14 Biggest Cities in the World by Population 5 Best Places to Visit in Thailand for Couples
a line of ships descending back to the era of Queen Elizabeth II from the 1960s Allure of the Seas Anthem of the Seas Best cruise ships in the world extravagant cruise ships in the world Freedom of the Seas Independence of the Seas largest cruise ships in the world largest passenger ships Liberty of the Seas most luxurious cruise ships in the world Norwegian Breakaway Norwegian Epic Norwegian Getaway Oasis of the Seas Quantum of the Seas Queen Mary 2 Slideshow The 11 Biggest Cruise Ships in the World the ocean's 10 biggest shipping companies
10 Countries with the Longest Coastline in the World
8 Best Places To Visit in Turkey Before You DIe
5 Best Places to Visit in Thailand for Kids and Families
5 Best Places to Visit in Thailand for Couples
11 Biggest Music Festivals in the World
14 Biggest Cities in the World by Population
11 Countries with the Best Climate in the World
6 Most Expensive Dog Food Brands
The 6 Countries with Most Nuclear Weapons
The 11 Countries with the Most Debt in the World


Coming up on Monday, I'll be participating in the February design bloggers link-up with Olioboard. The topic will be on Charity Causes. The Charity I chose to highlight is Housing Works. Although I am not directly affiliated with the Housing Works, Inc. organization, I wanted to share (here in Part 1 of a 2 part post) how I learned about them -- especially since I don't even live in NYC. It goes back to one of my initial blog connections when I first started blogging back in late 2009, and began my social media connections (especially on Twitter) in early 2010. I don't remember how, but I had started tweeting with Stan Williams, author of the blog, The Elegant Thrifter. We tweeted during the Academy Awards (which reminds me: we have a Twitter-versary coming up!) and I learned he had collaborated with an organization for his book called "The Find." I enjoy thrifting, but this was the first book I ever bought demonstrating the potential of trash to treasures, flea market adventures, upcycling & repurposing plus thrifting good finds... The organization Stan had collaborated with was/is called Housing Works.
About a year or so later, Jeff and I were going up to NYC for the 911 ten year anniversary. I didn't get to meet Stan (I think he was visiting family in Kansas at the time) but I did make it a point to visit one of the 12 Housing Works Thrift Shops located throughout New York City. Zaggat reviews Housing Works Thrift as, "Tremendous finds await the patient picker at this never stagnant local chain considered the Bergdorf of thrift outfits, where browsers happen upon super-fashionable vintage and contemporary clothing and designer furniture, all tended by pleasant, unobtrusive staffers."
What is Housing Works? Housing Works is a Non-Profit organization committed to ending the twin crises of AIDS and homelessness. Housing Works believes that all people have the right to a rich and empowering life. Housing Works raises money through donated goods at their 12 Housing Works Thrift shops locations in NYC/Brooklyn. Housing Works Thrift sells vintage and contemporary goods to fashionable bargain hunters. 100% of their proceeds fund their fight to end AIDS and homelessness (see Proceeds). Housing Works began in 1990. Housing Works Thrift launched in 1992. And last year, I learned of a charity event Housing Works holds to increase their awareness and raise funds through donations + auctions: The Design On A Dime Benefit. Since 2005, the Housing Works Design on a Dime Benefit has raised more than $2 million in support of Housing Works.
Each year of the event, Housing Works invites interior designers to participate in creating rooms for auction - or "vignettes" - of donated goods... But these goods are not used garment or household donations from city dwellers. These donations come from showrooms, vendors and manufactures in the furniture design industry. The vignette items are sold through auctions. All proceeds from a silent auction benefit Housing Works Jefferson Avenue Residence, a new housing development in Brooklyn for homeless adults living with HIV/AIDS which opened in summer 2012. I learned about the Design On A Dime event - again from an online connection - a spectacular designer and heart-felt AIDS advocate in NYC named Patrick J. Hamilton.
New York Designer Patrick J. Hamilton with Evette Ríos, Design on a Dime Co-Chair.
The Housing Works Design On A Dime Benefit debuted in 2004 and has gained notoriety ever since. I'm sure as last year, it will only be continuing to grow as a successful Housing Works fund raiser to further help facilitate their goal of ending the twin crises of AIDS and homelessness. Click here to watch a video from The Editor at Large at the DOAD event . Some of the Housing Works Design on a Dime Donors include: 1stDibs.com, Arteriors Home, Ballard Designs, Bernhardt Designs, Bloomingdale's, Bungalow 5, Calvin Klein Home, Crate & Barrel, Ikea, Jonathan Adler, Joss & Main, Nest Interiors, One Kings Lane, Ralph Lauren, Scalamandre, Seren & Lily, West Elm, Williams-Sonoma Home, V&M (Vintage Modern), and Z Gallerie -- just to name a few. See more donors here: Donors -- Housing Works. Some of its incredible sponsors have included: House Beautiful, Homegoods, Traditional Home, Veranda, Valspar and Elle Decor. Some of the events celebrity attendees have included Iman, Charlotte Moss, Genevieve Gorder, Andy Cohen, Alex McCord and... Nate Berkus!
I didn't realize it, but even one of my favorite thrift makeover mavens is a chairperson for the Design On A Dime Event: The Dumpster Diva and Fab Flea Finder herself - Lara Spencer. Lara holds charity "Taste Maker Tag Sales" of her vintage finds on the website One Kings Lane and donates all profits to Housing Works, a non-profit that helps those living with HIV/Aids. Lara says the Housing Works Design on a Dime Event "is as big as The Oscars."
Lara Spencer with ABC's Sam Champion and HGTV's Sabrina Soto at Design On A Dime 2012.
Since I personally LOVE using Pinterest to collect furnishings and then apply them to online design boards for projects using Olioboard, I thought this would be a good opportunity to demonstrate this wonderful cause + event during the Olioboard February Charity Causes link-up (which will be Part 2 of this post). I hope you'll join me Monday, plus the other 9 design bloggers -- as they each share their use of Olioboard to to support their charity causes.


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New EU Trade Deal Stirs Controversy in Cameroon
Posted by Admin | Aug 19, 2016 | Business | 0 |
In Cameroon, a new trade deal with the European Union is stirring up controversy. Other Central African states have refused to ratify the agreement, saying it was neither fair nor balanced. Civil society activist Emmanuel Mbami addressed a small crowd gathered near Cameroon's Ministry of Finance Thursday, protesting the new trade deal. Mbami said Cameroonian President Paul Biya was motivated by his desire to stay in office. He said Biya was afraid if he didn't sign the deal, he would lose the support of European leaders. Biya, he added, has shown Cameroon to be shameless state that would betray the other countries in the region. In late July, leaders in the Central African bloc CEMAC met in Malabo, capital of Equatorial Guinea, and decided not to sign the proposed trade agreement. But then, on August 4, Cameroon ratified the agreement on its own. 'Trade balance will not be there' Lawmaker Njong Evaristus says Cameroon was wrong because the agreement favors Europe. "We are tilting towards the Asian countries, China and the rest, and if you have to clear the custom duties for European goods, it is going to affect us so much because, first, our budget depends on the custom duties," Evaristus said. "We are a third-world country. We don't have developed industries that are up to a level that they can compete with the industries from Europe. In that case, you cannot balance the economy since we are a country that is dealing with agricultural products and the rest, and they are dealing with goods which are already finalized goods. The trade balance will not be there." The European Union is Cameroon's top trade partner, accounting for 35 percent of imports. The Economic Partnership Agreement, or EPA, removes customs duties on imports from the EU. The EU argues that the agreement is favorable to developing nations as it also allows countries to export goods to the EU without tariffs or maximum quotas under World Trade Organization rules. EU claims 'development agreement' Francoise Collet, head of the EU delegation to Cameroon, says the country will benefit from the deal. "It will be a safer environment for trade, and for expanding trade between our regions but also for expanding trade within the regions. That's one of our hopes," Collet said. "It is not only a trade agreement, it is also a development agreement. That is the aim of the EPA everywhere in the world and including Cameroon." African countries had until October 2014 to ratify the EPA, without which preferential treatment being given to their exports to European markets were to be suspended. Central African nations were able to get the deadline extended to August 1, 2016. Cameroon's former minister of economy and current minister of public works, Emmanuel Nganou Ndjoumessi, is part of the negotiating team. He told VOA they saw no reason to disrespect their commitment. "We are ratifying [the deal] in order to continue selling our goods in this market without paying customs duties. Cameroon has to respect its international engagements," he said. "We have been selling to the European Union without paying custom fees, consequently, we could easily sell our banana, our chocolates, our beans and so on." The five other CEMAC countries which refused to sign on to the deal are Gabon, the Central African Republic, Chad, the Republic of the Congo and Equatorial Guinea. At its last meeting, CEMAC asked Gabonese President Ali Bongo to meet with President Biya to discuss the matter.
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Omeprazole Tablets v Granules: Which is best?
How will you ever give such formulation to a horse? Fortunately, there are ways on how you can administer the medicines successfully.
A diagnosis has been established and everything will be up to you. Your vet may have prescribed to you omeprazole tablets and then you went on to buy some and saw how large the tablets can be. With Abler, there are various dosage forms that may suit your ability to give horse medications. So, between Abler's equine omeprazole tablets and granules, which would be easier to deliver? First, let's discuss these two equine omeprazole dosage forms – the tablets and the granules.
Anyone who's medicating a horse understands how these animals can be fussy when fed with modified feed. Still, you can lure your horse into eating the feed anyway. The trick is to find a carrier feed that it fancies. Try blending the granules with cooked carrots or with applesauce and mix it well with the grain. If your horse is particularly a sweet-tooth, add molasses or corn syrup.
If you don't consider sticking your arm inside the horse's mouth to deliver the medication, then the balling gun can just come in very handy. This device is intended to deliver medications in tablet or pill form, in this case, Abler's omeprazole tablets. To use this, pull back the plunger and insert the tablet on the open end. Hold it down so the tablet won't fall off. Raise your horse's head then insert the device into its mouth up to the base of his tongue. Push the plunger to deliver the tablet. Remember not to insert the balling gun beyond the base of the tongue as it can cause serious damage to its throat.
With both dosage forms presented, which would be easier to administer?
It may take trial and error and some time to find the best method of giving the medication to your horse. Some horses are hard to be tricked by masking granulated omeprazole with additives such as syrups, as mentioned above. Some horses would even resist when they are being touched near the mouth area. The best and easiest way to administer your equine omeprazole is to find out which of these methods will suit your horse best and which of them will not give you a hard time.
Abler will deliver only the best to you with its equine omeprazole formulations. Choose from equine omeprazole paste, granules, and tablets! Make Abler your trusted source for equine medications!


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Biocell vol.36 no.3 Mendoza dic. 2012
Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei
Randi Zukas1, Alex J. Chang1, Marian Rice2, Amy L. Springer1*
1 Department of Biology, Amherst College, Amherst, MA, USA
2 Department of Biological Sciences, Mount Holyoke College, South Hadley, MA, USA
*Address correspondence to:
Amy L. Springer.
Department of Biology, Amherst College, Amherst, MA, USA. E-mail: aspringer@amherst.edu
ABSTRACT: Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the a heavy chain, and IC138, an intermediate chain, cause severe motility defects including immotility. To determine if motility defects result from structural disruption of the axoneme, we used two different flagellar preparations to carefully examine axoneme structure in these strains using transmission electron microscopy (TEM). Our analysis showed that inner arm dynein size, axoneme structural integrity and fixed central pair orientation are not significantly different in either knockdown culture when compared to control cultures. These results support the idea that immotility in knockdowns affecting DNAH10 or IC138 results from loss of dynein-f function rather than from obvious structural defects in the axoneme.
Key words: Flagellar motility; Transmission electron microscopy; Central pair
Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness in humans and chronic wasting disease in sheep and cattle (Garcia et al., 2006). Current treatments for these diseases have many drawbacks and at present there is great need for new drugs(Steverding, 2010). The T. brucei flagellum is critical for motility, cell morphogenesis, polarity, sensing and cell division (Gull, 2003; Ralston et al., 2009; Rodríguez et al., 2009), thus this organelle is a potential target for anti-trypanosomal compounds. The T. brucei flagellum consists of a cylindrical"9+2" arrangement of nine doublet microtubules encircling a pair of singlet microtubules. This arrangement is highly conserved among eukaryotic flagella (Ralston and Hill, 2008). Cell motility results from flagellar bending caused by dynein-driven sliding of doublet microtubules against one another (Porter and Sale, 2000). Dyneins are arranged in repeating units along the inner and outer face of the axoneme and are referred to as the inner and outer arms, respectively. Dyneins are complexes of several polypeptides, the largest of which are the heavy chains (HC) that contain motor domains (Gennerich and Vale, 2009). In the green alga Chlamydomonas reinhardtii, the inner and outer arms are thought to have different roles in flagellar movement: outer arm dyneins (OAD) primarily generate force (Ishikawa et al., 2007), and inner arm dyneins (IAD) determine the size and shape of the flagellar bend (Kotani et al., 2007). Overall, production of a flagellar beat requires a precise coordination of all dyneins in the axoneme (Wirschell et al., 2007; Lindemann and Lesich, 2010).
Despite the observed structural conservation of the axoneme, the T. brucei flagellum has some unique features, such as its attachment along the cell body, the presence of a paraflagellar rod (PFR) that runs along its length (Bastin et al., 1996) and the tip-to-base bihelical beat (Ralston et al., 2009; Rodríguez et al., 2009). Furthermore, previous studies have suggested differences in roles of orthologous dynein components between T. brucei and C. reinhardtii or other eukaryotic flagella (Ralston et al., 2006; Baron et al. 2007a). While comparisons of these components between organisms are helpful for understanding trypanosome flagellar structure, the functions of axonemal components may vary and for this reason T. brucei flagellar components should be studied specifically.
Dynein-f, is an IAD thought to play an important role in regulating flagellar waveform (Kotani et al., 2007). We previously reported that silencing of the dynein-f a-HC gene (dnah10) in insect-stage T. brucei cells caused flagellar immotility as well as growth, cell division and morphological defects (Springer et al., 2011), but did not see any structural changes by electron microscopy. To extend this analysis, we compared two different axonemal preparation processes using strains with RNAi knockdown of dnah10 or of the gene for another dynein-f component, ic138. Flagellar preparations showed no significant differences in size of IAD, axonemal integrity or central pair orientation for either RNAi knockdowns or for either preparation method. This thorough TEM analysis demonstrates that the immotility phenotype is not a result of major structural defects and is more likely caused by the loss of dynein-f function after knockdown, suggesting that IAD function is critical for flagellar movement in trypanosomes.
Culturing and induction
T. brucei DNAH10RNAi has been described previously (Springer et al., 2011). IC138 RNAi was constructed in the same manner: a portion from nucleotides 453-868 of Tbic138 (Tb927.2.4060; 5' primer: GATGTTGTTCCAATTCCATCC, 3' primer: TCCACGGTGTGACTTGTAACC), was amplified by PCR and ligated into HindIII and XhoI sites of pZJM (Wang et al., 2000). This construct was stably transfected into the 29-13 strain (Wirtz et al., 1999), grown in SDM-79 medium (Brun and Schönenberger, 1979), and clonal lines were established as described (Chandler et al., 2008). To induce RNAi, tetracycline was added to 1 µg/ml 48 hours prior to harvesting, and growth was monitored by hemacytometer. RNA isolation and real-time RT-PCR analyses were carried out as described (Springer et al. 2011) using the following ic138 primers. Primer set 1: 5'primer: TTGCCT TTTCCGACGAGCAT, 3' primer: CCCATTTCAG CTCCCACACC, product size 156 bp. Primer set 2: 5'primer: CAGTGGCGTGGATGTTTTCG, 3' primer: AGACCTGACGTTGCCGCTTC, product size 163 bp.
Time-lapse motility assay
Log phase cultures were harvested and split to 1x106 cells/mL and incubated at room temperature for one hour. 10mL of cell culture was added to a poly-L-glutamate-coated slide chamber (Gadelha et al., 2005), sealed with vaseline around the coverslip. Time-lapse images were generated by compiling 30 sequential images at 1 second intervals using a Nikon Eclipse E400 (nikon.com) under 20X objective, and outfitted with Spot Insight QE software (SPOT Imaging Solutions, diaginc.com). In general, dark field produced the best results for minimizing background and optimizing contrast of the cells, but every slide required special adjustments to optimize the image. Images were made black and white and the threshold was adjusted to recognize cells against the background. The compiled images were converted into motility traces with the Computer Assisted Sperm Analyzer (CASA) plug-in (Wilson-Leedy and Ingermann, 2007) for ImageJ (Abramoff et al., 2004), run with the following parameters: minimum sperm size 20 pixels, maximum sperm size 1500 pixels, minimum track-length 2 frames, VSL, VAP and VCL all set to zero, and 30 frames per second. Occasionally, the minimum track-length was modified to produce more fluid traces of moving cells. After analysis, each trace was verified for accuracy using the original image before being classified as runner (cells with progressive movement for at least 5 seconds), tumbler (cells with flagellar beating, but no progressive movement), or immotile (cells with no net movement orflagellar beat) as described (Springer et al., 2011).
Flagellar preparation
Flagellar preparation using the one-step extraction protocol has been described (Robinson et al., 1991; Oberholzer et al., 2009). Comparisons are made to flagella prepared previously from the two-step protocol (Springer et al. 2011). Approximately 2 x 108 cells were used for DNAH10RNAi preparations, and 1.5 x 107 cells for IC138 RNAi preparations.
Extracted flagella were prepared as described (Springer et al. 2011), and images were viewed on a Philips CM100 transmission electron microscope (FEI.com), captured digitally or scanned from Kodak Electron microscope film no. 4489 (Aremac.com).
Scoring axoneme images
IAD structures were counted and scored by size as described previously (Springer et al., 2011). An axoneme was considered"broken" if it had no central pair, had a severely deformed shape, or if the circumference of microtubules was disrupted. Central pair (CP) orientation was assessed as described (Ralston et al., 2006). Briefly, axoneme images in which the central pair was clearly discernible were selected randomly. A reference line was drawn from the center point of doublet 1 to that of doublet 7. A second line was drawn that bisected the central pair microtubules. The acute angle of intersection between these two lines was measured. The median angle was taken from the angles of all uninduced preparations, and the deflection from the median was recorded for each axoneme in the sample. Contrast and brightness adjustments were made in Adobe Photoshop CS3 (adobe.com) to increase IAD visibility. The IAD color density was not taken into account when making these assessments, since it was not possible to distinguish between differences due to variation in IAD size or to unequal stain distribution.
The gene Tb927.2.4060 encodes the predicted ortholog to intermediate chain IC138 (Wickstead and Gull, 2007), and was silenced using tetracycline-inducible RNAi as described in Materials and Methods. Knockdown of ic138 mRNA was confirmed by real time PCR analysis: by 48 hours post-induction the transcript levels were reduced to 27% of that seen in uninduced cultures (Fig. 1A). To monitor the effect of this knockdown, a motility assay was used to characterize the movement of individual cells from cultures in which RNAi was induced or uninduced. Motility traces were recorded using time-lapse microscopy (Fig. 1B) in which individual trypanosomes were observed for thirty seconds and classified as runner, tumbler or immotile as described in Materials and Methods. When compared to uninduced cells, induced IC138RNAi showed a significant decrease in the percentage of runners, and an increase in percentages of both tumblers and immotile cells. By 48 hours post-induction, the immotile category is the largest at 43%, and only 5% of cells are runners.
Figure 1. Analyses of IC138 RNAi knockdown cells. A. Relative transcript levels of ic138 RNA by real time RT-PCR analysis. Gene expression was normalized to the housekeeping genes gapdh and rps23. The level of gene expression in uninduced cells (-Tet) was set as 1.0, and the relative expression in tetracycline-induced IC138RNAi cells (+Tet), from each of two different primer sets, is shown (Primer Set1, 0.268; Primer Set2, 0.277). Data represent averages from two independent sets of uninduced and induced cultures with standard deviations indicated by error bars. B. Summaries of traces from time-lapse motility assays on IC138RNAi. Pie charts with percentages of cells scored as runners (white), tumblers (gray) or immotile (black) in cultures that were either uninduced (above), or induced with tetracycline and grown 48 hours before analysis (below). Above each chart, the n represents the raw number of cells scored for each culture condition.
In order to thoroughly assess the effect of dnah10 or ic138 knockdown on axoneme structure, we used the one-step preparation (Oberholzer et al., 2009). The one-step method yields more complete axoneme structures, unlike the two-step method which removes OAD and structures in the axoneme center but leaves inner arms intact and more clearly discernible (Fig. 2). DNAH10RNAi and IC138RNAi cultures were induced for 48 hours, a time point at which a large percentage of IC138RNAi cells (Fig. 1), and a distinct majority of DNAH10RNAi cells (Springer et al., 2011) are immotile.
Figure 2. Representative axoneme images from one-step (A) and two-step (B) preparations. Both preparations remove flagellar membranes but axonemes from the one-step preparation retain the OAD, radial spoke and central pair structures. Axonemes from the two-step preparation have lost these structures, yielding an unobstructed view of the IAD structures. In the two-step preparation, the PFR in often missing or broken as shown here, this is likely due to residual protease activity (possibly contaminating the DNase reaction), not to the salt extraction per se (Oberholzer et al., 2009). Scale bar is 200 nm.
Flagellar fractions were always prepared simultaneously on RNAi-induced cultures and uninduced cultures that had been cultured in parallel. Flagella were prepared from both IC138RNAi and DNAH10RNAistrains; for the latter, two preparations were completed independently. The resulting images were compared to images of DNAH10RNAi from a two-step preparation described previously (Springer et al., 2011). All axoneme preparations were scored for number of IAD by size (Fig. 3) and results are shown in Table 1. To assess whether the RNAi knockdown led to any overall loss of structural integrity, the fraction of broken axonemes relative to intact axonemes was measured from each sample set (Table 1). For both criteria, there were no significant differences between samples in which RNAi had been induced and the corresponding uninduced samples, as determined by Chi-square analysis.
Figure 3. Representative axonemes from various preparations. Uninduced and RNAi-induced samples were prepared for TEM using the one-step preparation or two-step preparation. Four representative film images of individual axonemes are shown for each preparation. (A,B) Two-step preparation of DNAH10RNAi uninduced and knockdown induced samples, respectively. (C,D) One-step preparation of DNAH10RNAi uninduced and induced samples, respectively. (E,F) One-step preparation of IC138RNAi uninduced and induced samples, respectively. An example of an IAD scored as intact or large is shown with an arrow, an IAD scored as small is shown with an arrowhead. Scale bar is 100 nm.
Table 1. Summary of inner arm dynein size and axoneme integrity analysisa
The CP microtubules maintain a fixed orientation in the T. brucei axoneme relative to extra-axonemal structures such as the PFR (Ralston et al., 2006; Branche et al., 2006). Because the one-step method leaves the PFR-axoneme intact in most cases, these preparations provide a better opportunity for scoring CP orientation relative to PFR orientation. At least 25 axonemes were scored from each RNAi induced and uninduced preparation. An overall median angle of 61º was determined from all measurements of uninduced samples from either strain. For each axoneme, the CP angle was measured and the deflection from the median angle was recorded (Fig. 4 and Table 2). It appeared that the range of angles might have been greater in the RNAi-induced samples relative to the respective uninduced samples. In order to test this statistically, we employed two statistical tests: The Wilcoxon rank sum test (Wild and Seber, 1999) the Kolmogorov-Smirnov test for two-independent samples (Daniel, 2000). These do not require normal distributions, since our sample sizes were small and not all datasets generated normal distributions. For each preparation we compared the measured deflections from the median for each RNAi-induced preparation, to those from the total uninduced sample set, using P<0.05 as the a level of statistical significance. In both tests, the differences in range of deflection values were not significant between induced and uninduced samples.
Figure 4. Distribution of central pair orientation angles deflected from the median angle of 61 degrees. A dot plot representing the deflection of the central pair-PFR angle from the median for each individual axoneme is shown for each preparation. Nomenclature for labeling of axoneme preparations is described for Table 1.
Table 2. Summary of central pair orientation analysisa
We have presented a structural analysis of axonemes from T. brucei strains in which dnah10 or ic138 has been knocked down. Extensive TEM analysis of axoneme images, comparing the one-step (less stringent) preparation to the two-step (more stringent) preparation, shows that no gross structural defects are evident in these strains that show a severe motility defect. These results are consistent with the interpretation that immotility in these strains is a consequence of the loss of function of the targeted dynein-f components, perhaps including the loss of other associated axonemal proteins. This suggests that proper IAD function is necessary for motility.
It was not surprising that the knockdown of a single HC such as DNAH10 did not cause a visible change in size by TEM: IAD structures are massive, containing 6-8 HCs, thus the loss of one HC is a relatively minor change in the overall IAD electron density (Kamiya et al., 1991). In addition to IAD size, we assessed axonemal integrity and CP orientation since this structure in T. brucei maintains a fixed orientation. While dynein-f is not expected to interact with CP directly, the protein complexes that transduce the CP signal lie near the base of the dynein-f complex (Lindemann and Lesich, 2010) and loss of DNAH10 or IC138 could prevent the assembly of multiple proteins. Indeed in C. reinhardtii, loss of DNAH10 or IC138 has been shown to affect the assembly of other proteins (Myster et al., 1997; Bower et al., 2009). It is possible that loss of either of these proteins could destabilize a complex that helps in some way to maintain the CP orientation. This orientation can be more effectively examined in the one-step preparation since the CP and PFR are more intact. Our results suggest that the RNAi-induced strain might yield a greater range in CP angles, but this difference in range was shown not to be statistically significant using two different non-parametric tests. While it is possible that the extent of the knockdown in each culture might affect this result, overall we see no significant disruption in CP orientation in these strains. Although we see no structural disruption of axonemes in these strains, we still believe that TbDNAH10 and TbIC138 are flagellar proteins. Both proteins were predicted to be orthologs of known flagellar proteins based on comprehensive phylogenetic analysis (Wickstead and Gull, 2007) and both proteins have been identified as components of flagella in independent proteomic and genomic analyses of T. brucei flagella (Broadhead et al., 2006; Baron et al., 2007b). Because axonemal components have been highly conserved during evolution (Holzbaur and Vallee, 1994), these orthologs can be reasonably assumed to form equivalent structures in different organisms. Therefore we are confident that these proteins reside in the T. brucei flagellum.
In C. reinhardtii, DNAH10 is thought to play an important role in regulating flagellar waveform (Kotani et al., 2007); it exhibits slower gliding velocities than other dynein HCs and is oriented in a more oblique angle relative to the microtubule than other inner arm HCs (Bui et al., 2008). C. reinhardtii dynein-f is thought to inhibit the extent of sliding of adjacent microtubules and modulate the amount of curvature of the flagellar bend (Kotani et al., 2007). C. reinhardtii IC138 is a regulatory protein, inhibited by phosphorylation and thought to negatively regulate DNAH10 (Wirschell et al., 2007). Because of these roles, knockdown of either of these proteins might reasonably be expected to interfere with normal coordination of dynein movement in flagellar bending, and our results suggest that a complete inner arm is necessary for flagellar beating in T. brucei. Further analyses of these strains will extend our understanding of their roles in regulating bending in the T. brucei flagellum.
This study presents an approach that could be generally useful to assess integrity and quality of axoneme structure using TEM in flagellar mutants. Our analysis provides evidence that the immotility in dnah10 and ic138 knockdowns results from disrupting coordination of flagellar movement not overall flagellar structure.
We thank Sue Lancelle for assistance with sample preparation, Sulynn Machado for assistance with culturing, Amherst College Biology Department for support, and Dr. Shu-Min Liao for statistical consulting.
1. Abramoff MD, Magalhães PJ, Ram SJ (2004). Image processing with ImageJ. Biophotonics International 11: 36-42. [ Links ]
2. Baron DM, Kabututu ZP, Hill KL (2007a). Stuck in reverse: loss of LC1 in Trypanosoma brucei disrupts outer dynein arms and leads to reverse flagellar beat and backward movement. Journal of Cell Science 120: 1513-1520. [ Links ]
3. Baron DM, Ralston KS, Kabututu ZP, Hill KL (2007b). Functional genomics in Trypanosoma brucei identifies evolutionarily conserved components of motile flagella. Journal of Cell Science 120: 478-491. [ Links ]
4. Bastin P, Matthews KR, Gull K (1996). The paraflagellar rod of kinetoplastida: solved and unsolved questions. Parasitology Today 12: 302-307. [ Links ]
5. Bower R, VanderWaal K, O'Toole E, et al. (2009) IC138 defines a subdomain at the base of the I1 dynein that regulates microtubule sliding and flagellar motility. Molecular Biology of the Cell 20: 3055-3063. [ Links ]
6. Branche C, Kohl L, Toutirais G, Buisson, J, Cosson J, Bastin P (2006) Conserved and specific functions of axoneme components in trypanosome motility. Journal of Cell Science 119: 3443-3455. [ Links ]
7. Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, Gull K (2006) Flagellar motility is required for the viability of the bloodstream trypanosome. Nature 440: 224-227. [ Links ]
8. Brun R, Schönenberger, M (1979) Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semidefined medium. Acta Tropica 36: 289-292. [ Links ]
9. Bui KH, Sakakibara H, Movassagh T, Oiwa K, Ishikawa T (2008) Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella. Journal of Cell Biology 183: 923-932. [ Links ]
10. Chandler J, Vandoros AV, Mozeleski B, Klingbeil MM (2008) Stemloop silencing reveals that a third mitochondrial DNA polymerase, POLID, is required for kinetoplast DNA replication in trypanosomes. Eukaryotic Cell 7: 2141-2146. [ Links ]
11. Daniel WW (2000) Applied Nonparametric Statistics. Duxbury Press, Pacific Grove, CA. [ Links ]
12. Gadelha C, Wickstead B, de Souza W, Gull K, Cunha-e-Silva N (2005) Cryptic paraflagellar rod in endosymbiont-containing kinetoplastid protozoa. Eukaryotic Cell 4: 516-525. [ Links ]
13. Garcia A, Courtin D, Solano P, Koffi M, Jamonneau V (2006) Human African trypanosomiasis: connecting parasite and host genetics. Trends in Parasitology 22: 405-409. [ Links ]
14. Gennerich A, Vale RD (2009) Walking the walk: how kinesin and dynein coordinate their steps. Current Opinion in Cell Biology 21: 59-67. [ Links ]
15. Gull K (2003) Host-parasite interactions and trypanosome morphogenesis: a flagellar pocketful of goodies. Current Opinion in Microbiology 6: 365-370. [ Links ]
16. Holzbaur ELF, Vallee RB (1994) Dyneins: Molecular Structure and Cellular Function. Annual Review of Cell Biology 10: 339-372. [ Links ]
17. Ishikawa T, Sakakibara H, Oiwa K (2007) The architecture of outer dynein arms in situ. Journal of Molecular Biology 368: 1249-1258. [ Links ]
18. Kamiya R, Kurimoto E, Muto E (1991) Two types of Chlamydomonas flagellar mutants missing different components of innerarm dynein. The Journal of Cell Biology 112 : 441-447. [ Links ]
19. Kotani N, Sakakibara H, Burgess SA, Kojima H, Oiwa K (2007) Mechanical properties of inner-arm dynein-f (dynein I1) studied with in vitro motility assays. Biophysical Journal 93: 886-894. [ Links ]
20. Lindemann CB, Lesich KA (2010) Flagellar and ciliary beating: the proven and the possible. Journal of Cell Science 123: 519-528. [ Links ]
21. Myster SH, Knott JA, O'Toole E, Porter ME (1997) The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex. Molecular Biology of the Cell 8: 607-620. [ Links ]
22. Oberholzer M, Lopez MA, Ralston KS, Hill KL (2009) Approaches for functional analysis of flagellar proteins in African trypanosomes. Methods in Cell Biology 93: 21-57. [ Links ]
23. Porter ME, Sale WS (2000) The 9 + 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility. Journal of Cell Biology 151: F37-42. [ Links ]
24. Ralston KS, Hill KL (2008) The flagellum of Trypanosoma brucei: new tricks from an old dog. International Journal for Parasitology 38: 869-884. [ Links ]
25. Ralston KS, Kabututu ZP, Melehani JH, Oberholzer M, Hill KL (2009) The Trypanosoma brucei flagellum: moving parasites in new directions. Annual Review of Microbiology 63: 335-362. [ Links ]
26. Ralston KS, Lerner AG, Diener DR, Hill KL (2006) Flagellar motility contributes to cytokinesis in Trypanosoma brucei and is modulated by an evolutionarily conserved dynein regulatory system. Eukaryotic Cell 5: 696-711. [ Links ]
27. Robinson D, Beattie P, Sherwin T, Gull K (1991) Microtubules, tubulin, and microtubule-associated proteins of trypanosomes. Methods in Enzymology 196: 285-299. [ Links ]
28. Rodríguez JA, Lopez MA, Thayer MC, Zhao Y, Oberholzer M, Chang DD, Kisalu NK, Penichet ML, Helguera G, Bruinsma, R, Hill KL, Miao J (2009) Propulsion of African trypanosomes is driven by bihelical waves with alternating chirality separated by kinks. Proceedings of the National Academy of Sciences (USA) 106: 19322-19327. [ Links ]
29. Springer AL, Bruhn DF, Kinzel KW, Rosenthal NF, Zukas R, Klingbeil MM (2011) Silencing of a putative inner arm dynein heavy chain results in flagellar immotility in Trypanosoma brucei. Molecular and Biochemical Parasitology 175: 68-75. [ Links ]
30. Steverding D (2010) The development of drugs for treatment of sleeping sickness: a historical review. Parasites & Vectors 3: 15. [ Links ]
31. Wickstead B, Gull K (2007) Dyneins across eukaryotes: a comparative genomic analysis. Traffic 8: 1708-1721. [ Links ]
32. Wild CJ, Seber GAF (1999) Chance Encounters: A First Course in Data Analysis and Inference. John Wiley, New York. [ Links ]
33. Wilson-Leedy JG, Ingermann RL (2007) Development of a novel CASA system based on open source software for characterization of zebrafish sperm motility parameters. Theriogenology 67: 661-672. [ Links ]
34. Wirschell M, Hendrickson T, Sale WS (2007) Keeping an eye on I1: I1 dynein as a model for flagellar dynein assembly and regulation. Cell Motility and the Cytoskeleton 64: 569-579. [ Links ]
35. Wirtz E, Leal S, Ochatt C, Cross GA (1999) A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Molecular and Biochemical Parasitology 99: 89-101. [ Links ]
Received: June 14, 2012.
Revised version received: October 6, 2012.
Accepted: October 10, 2012.
Facultad de Ciencias Médicas, U.N.Cuyo
C.C. 56 (5500) Mendoza, Argentina
Fax: (54 261) 449-4117
http://www.cricyt.edu.ar/ihem/
biocell.journal@gmail.com


text:
Q: E: Unable to locate package php7.2-bcmath I'm trying to install package php7.2-bcmath on Ubuntu 14.4
sudo apt-get install php7.2-bcmath

but I keep getting this error:
Reading package lists... Done
Building dependency tree
Reading state information... Done
E: Unable to locate package php7.2-bcmath
E: Couldn't find any package by regex 'php7.2-bcmath'

I already try those options:
sudo apt-add-repository ppa:ondrej/php
sudo apt-get update
sudo apt-get install php7.2-bcmath

add this to /etc/apt/sources.list:
deb http://security.ubuntu.com/ubuntu artful-security main universe

Nothing works!
Please help

A: You should check which PHP version is available and if you addressed the correct package name.
Use:
apt-cache search php

to check, if PHP7.2 and all the extensions are available at your environment.
Determine the correct package name of the bcmath package by searching trough all available packages:
apt-cache search php | grep bcmath

If you see an bcmath package for php7.2 use this name with the sudo apt-get install .... syntax.


text:
Boko Haram abducts women in Borno
by TheCable  0 Comments
Some women were abducted along Pulka road in Gwoza local government area of Borno state on Tuesday evening.
The victims were on their way to a neighbouring local government when the insurgents attacked the bus they were in.
Muhammed Buba, one of the passengers on a vehicle that was attacked, told TheCable that many people sustained injuries in the attempt to escape.
Buba did not say if anyone was killed but added that there were sporadic gunshots from different directions during the attack.
Idrissa Hamisu, another victim, said the insurgents came in two Toyota Hilux buses.
"Many who were caught in the attack were women who lacked the strength to run away. The incident occurred around on Tuesday," he said.
Hamisu said the insurgents also engaged in serious gun battle with troops in the area.
"I don't know if the soldiers were able to save the passengers but many women were abducted in the attack,"he said.
Texas Chukwu, spokesman of the army, was not available for comments as his telephone line was out of reach when TheCable attempted to speak with him on Wednesday.
The attack comes 48 hours after many soldiers were reportedly killed in the north-east but the military denied the killings, describing the report as fake news.
boko harambornoGwoza
IPOB to Arewa youths: Do your worst.. we won't give out our ancestral land for Ruga
Lawan: Delay in appointment of ministers won't affect 2020 budget
⚏Top of the Day
Appeal court orders FG to release Dasuki, reviews his bail conditions


Avoid Vikings spears and try to get the best score. If you catch the viking head you will get extra points. Some spears is faster than other. If the spears hit you than you lose and you need to start all over. Get the best score and enjoy in this game. Use only your mouse to play this game. Play Viking Spears.


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Il Certificato di Destinazione Urbanistica (spesso chiamato semplicemente C.D.U.) è il documento rilasciato dalla pubblica amministrazione (tipicamente gli Uffici Tecnici Comunali) che contiene le indicazioni urbanistiche che riguardano gli immobili, più precisamente il fabbricato o il terreno interessato dal certificato.

Le indicazioni urbanistiche riportate in esso vengono estrapolate dal Piano Urbanistico Comunale oppure dal Piano Regolatore Generale, dalle cartine tecniche regionali più precisamente.

Il certificato di destinazione urbanistica indica i dati del fabbricato o del terreno (Foglio e Particella catastale), la destinazione urbanistica (es. se in zona residenziale o agricola), parametri urbanistici come l'indice di fabbricabilità (se possibile edificare), l'indice del dissesto dei versanti e l'indice di inondabilità.

Tale certificato è a titolo oneroso; inoltre è un documento ufficiale utilizzato nella maggior parte dei casi nelle compravendite immobiliari e nei contratti preliminari di compravendita di fabbricati e di terreni in quanto, come detto sopra, rappresenta una sorta di "Libretto di istruzioni", perché ne indica le zone di piano in cui ricade e da quelle indicazioni, con l'ausilio dei piani urbanistici, ne descrive le possibilità edilizie sull'immobile. Questo certificato è una garanzia per l'acquirente, che può verificare le possibilità di intervento sull'immobile.

Qualunque atto notarile avente a oggetto trasferimento di terreno, chiede obbligatoriamente (pena nullità dell'atto stesso) l'allegazione del CDU contenente le prescrizioni urbanistiche ed edilizie. Se il trasferimento immobiliare riguarda un terreno di pertinenza ad un fabbricato inferiore a 5000 metri quadrati, non è obbligatoria l'allegazione all'atto notarile.

Riferimenti normativi
Nella normativa vigente, il certificato di destinazione urbanistica è definito, nei suoi contenuti essenziali e generici e nel suo uso, validità e durata, all'articolo 30 del Testo unico dell'edilizia (DPR 6 giugno 2001, n. 380). In particolare esso contiene "le prescrizioni urbanistiche riguardanti l'area interessata". L'articolo 30 del sopracitato testo unico specifica anche la nullità degli atti tra vivi riguardanti compravendita di terreni qualora non sia allegato il relativo certificato di destinazione urbanistica:

"Gli atti tra vivi, sia in forma pubblica sia in forma privata, aventi ad oggetto trasferimento o costituzione o scioglimento della comunione di diritti reali relativi a terreni sono nulli e non possono essere stipulati ne' trascritti nei pubblici registri immobiliari ove agli atti stessi non sia allegato il certificato di destinazione urbanistica contenente le prescrizioni urbanistiche riguardanti l'area interessata. Le disposizioni di cui al presente comma non si applicano quando i terreni costituiscano pertinenze di edifici censiti nel nuovo catasto edilizio urbano, purché la superficie complessiva dell'area di pertinenza medesima sia inferiore a 5.000 metri quadrati".

Lo stesso articolo 30 specifica ulteriori requisiti ed eccezioni. Per quanto riguarda la durata del certificato, lo stesso articolo 30 specifica che "esso conserva validità per un anno dalla data di rilascio se, per dichiarazione dell'alienante o di uno dei condividenti, non siano intervenute modificazioni degli strumenti urbanistici".

La vendita forzata
L'uso del certificato di destinazione urbanistica per gli atti riguardanti vendita forzata è normato dall'articolo 591-bis del Codice di procedura civile e dalla Legge 3 agosto 1998, n. 302.

Dichiarazione di successione
Nella dichiarazione di successione (da presentare all'Agenzia delle entrate), il certificato di destinazione urbanistica è stato sostituito dalla dichiarazione sostitutiva di atto notorio, in seguito alla cosiddetta "decertificazione". Ciò non toglie che il certificato di destinazione urbanistica venga richiesto "per uso successione" con l'esenzione dei tributi, principalmente al fine di ricevere le informazioni che poi andranno inserite nella dichiarazione sostitutiva.

Note

Altri progetti

Collegamenti esterni

Il portale UrbisMap è un servizio gratuito per consultare l'Urbanistica, i Piani Territoriali ed il Catasto. La versione avanzata del servizio permette ai Comuni Italiani di produrre in modo immediato i Certificati di Destinazione Urbanistica.

Diritto urbanistico italiano


On Monday, November 21st, President Obama signed into law the Three Percent Withholding Repeal and Job Creation Act, and the VOW to Hire Heroes Act. The new legislation permanently repeals the three percent withholding requirement on government payments to contractors and adds new tax credits for hiring military veterans. The IRS's levy authority over payments to federal vendors is expanded, and increased information exchange between the IRS and the Department of Veterans Affairs has been authorized.
The Tax Increase Prevention and Reconciliation Act of 2005 enacted a mandatory withholding of three percent on payments made to contractors by federal, state and local governments after December 31, 2012. The regulation would have been burdensome to businesses and governments and has been permanently repealed.
Repeal of this provision will cost an estimated 12.8 billion in revenue. As an offset, the bill includes a revision of the definition of modified adjusted gross income for the purposes of determining eligibility for the health insurance premium assistance credit.
Part of Obama's American Jobs Act, the VOW to Hire Heroes Act enhances the existing Work Opportunity Tax incentives for hiring veterans with two new credits. The Returning Heroes Tax Credit rewards employers with credits of up to $5,600 for hiring job–seeking veterans. A Wounded Warrior Tax Credit of up to $9,600 is available to employers hiring veterans with service–connected disabilities who have been looking for work for more than six months.
The new law expands IRS levy authority with respect to payments made to federal vendors with unpaid tax liabilities. Such vendors will be subject to continuous IRS levies against 100% of any payment due them from the federal government, including payments due for good or services sold or leased.
A number of non–tax initiatives have been enacted that are aimed at making it easier for soldiers to transition back into the civilian workplace. The new programs include career counseling, education and training and are expected to reduce the high jobless rate for post 9/11 veterans.


NAS: its bars and erected its pillars.
KJV: thereof, and reared up his pillars.
KJV: and they set him between the pillars.
NAS: with cedar beams on the pillars.
KJV: with cedar beams upon the pillars.
NAS: So the work of the pillars was finished.
KJV: so was the work of the pillars finished.
KJV: and like unto these had the second pillar with wreathen work.
NAS: which were on the pillars.
KJV: which [were] upon the pillars.
KJV: out of her place, and the pillars thereof tremble.
NAS: It is I who have firmly set its pillars. Selah.
KJV: I bear up the pillars of it. Selah.


Te Syrian-born artist, now in her seventies who was raised in Lebanon and now settled in California presents a lovely show at the Kaufmann Repetto Gallery in Chelsea. Slender pedestals present an array of stoneware sculptures. The shapes and colors vary - from sky blue, olive, brown and white.


text:
American museums preparing to sell certain masterpieces
Brooklyn Museum to Sell 12 Works as Pandemic Changes the Rules… the title of Robin Pogrebin' article in the New York Times on 16 September after the announcement of the Brooklyn Museum's decision to sell twelve works from its permanent collections.
Faced with the crisis and despite reopening a few days earlier, the Brooklyn Museum had to take this painful decision in order to pay its operating costs… a question of survival. After several weeks of closure, there simply aren't enough visitors to pay the bills, especially as tourists are still almost completely absent. The Brooklyn Museum needs $40 million for the maintenance of the collections and the payment of its salaries. In this emergency situation, agreeing to part with a few works is indeed the most effective solution.
Until now, sales of museum works were tolerated by the Association of Art Museum Directors (AAMD) in the context of 'disaccessions' or 'deaccessioning' with a view to reinvesting the proceeds into new acquisitions deemed more 'relevant' for the museum. But the powerful Association has relaxed this rule, announcing that it would not penalize museums that resell part of their collection "to pay for expenses related to the maintenance of the collections", a new temporary regime that has been given a provisional end-date of 10 April 2022. Many museum works are therefore likely to hit the market over the next two years.
For now, the Brooklyn Museum is parting with 12 important works through Christie's New York, as part of the Old Masters sale scheduled for 15 October 2020. The sale's star work (and a painful loss for the American museum) is a superb Lucretia painted by Lucas I CRANACH (see picture above) in 1526 expected to fetch between $1.2 and $1.8 million. The estimate is conservative for this work which could climb much higher because the subject is attractive, the provenance superb and Cranach's prices are currently buoyant.
Among the masterpieces being sold off by the Brooklyn Museum in mid-October are Italienne debout tenant une cruche by the French painter Camille Jean-Baptiste COROT (estimated $200,000 – $300,000), a portrait attributed to Lorenzo Costa (estimated $60,000 – $80,000) and a large Saint Jerome by Donato de' Bardi, active in the 15th century ($80,000 – $ 120,000). There will also be a painting by Gustave COURBET. In total the works are expected to generate between $2.3 and $3.5 million.
In November 2019, the same museum raised $6.6 million from the sale of a Pope (1958) painting by Francis BACON at Sotheby's. They are therefore not new to the exercise. What is different is the relaxation of the professional ethical rules on deaccessioning.
In France, where French public collections are declared "inalienable, imprescriptible and unseizable" (except for restitutions), this drastic solution is not even an option. The major French museums (especially in Paris) who are also in serious trouble because of the health crisis, therefore cannot resolve their budgetary problems in this way.
The Louvre, for example, has posted financial losses of 59 million euros since the start of the crisis. This year's visitor numbers at the famous museum rose from 220,000 in July to 330,000 in August, versus 800,000 per month in the summer months of previous years. Once the health crisis set in, this sharp contraction was of course predictable since foreigners make up the bulk of visitors to the Louvre (75%). Despite this very difficult situation, there are no plans to relax the French regulations governing museum collections.
Above right : Portrait attr. to Lorenzo Costa (coll Brooklyn Museum)


text:
Definition of Alfred. Meaning of Alfred. Synonyms of Alfred
Here you will find one or more explanations in English for the word Alfred. Also in the bottom left of the page several parts of wikipedia pages related to the word Alfred and, of course, Alfred synonyms and on the right images related to the word Alfred.
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- Alfred may refer to: Alfred J. Kwak, Dutch-German-****anese anime television series Alfred (Arne opera), a 1740 masque by Thomas Arne Alfred (Dvořák),...
- who died when Alfred was young. Three of Alfred's brothers, Æthelbald, Æthelberht and Æthelred, reigned in turn before him. Under Alfred's rule, considerable...
- Sir Alfred Joseph Hitch**** KBE (13 August 1899 – 29 April 1980) was an English filmmaker who was one of the most influential figures in the history of...
- Alfred Lewis Enoch, also credited as Alfie Enoch (born 2 December 1988) is a British actor, best known for playing Dean Thomas in the Harry Potter film...
- Alfred Ernst Rosenberg (12 January [O.S. 31 December 1892] 1893 – 16 October 1946) was a Baltic German **** theorist and ideologue. Rosenberg was first...
- Alfred Bernhard Nobel (/noʊˈbɛl/ noh-BEL, Swedish: [ˈǎlfrɛd nʊˈbɛlː] (listen); 21 October 1833 – 10 December 1896) was a Swedish chemist, engineer, inventor...
- Alfred Thaddeus Crane Pennyworth or simply Alfred is a fictional character appearing in American comic books published by DC Comics, most commonly in...
- Alfred Nu Steele (April 24, 1900 – April 19, 1959) was an American soft drink businessman He graduated from Northwestern University in 1923, where he pla****...
- Alfred Molina (born 24 May 1953) is a British actor known for his work on the stage and screen. He first rose to prominence in the West End, earning a...
- Alfreð Gíslason (born 7 September 1959) is an Icelandic handball coach and former player. He is the current head coach of the German men's national team...
PaleolithPalestPalmariumPalmatelyPalmcristPalmisterPalsyingPalterlyPamperosPanPan-AnglicanPancratiast
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The Tea Explorer documentary: links - Hawaiian Trail & Mountain Corp.
We had a wonderful turn out for our movie night documentary The Tea Explorer! The film was both education and inspirational and looked especially impressive projected on the side of the clubhouse on our 12 foot by 9 foot screen. Thank you all for coming and sharing the evening together and learning more about Jeff Fuch's interest in sharing the art of tea growing in Yunnan China and his travels along the historic tea road linking China, Nepal and Tibet.


Lockouts are at a time-based rate. You decide the amount of people and the amount of time per game. You can have up to 28 people in the arena at one time and only your group is allowed in the arena during that time.
If you would like to make a reservation you can call us at (916) 971-4040 or send us a request here.


text:
CHN Aircraft Carrier Liaoning
a photo feature by Kostas Katseas of a Albatros Productions kit
Kostats Katseas shares another one of his 1/1250 scale creations. This time one of Albatros Models CHN Aircraft Carrier 16 Liaoning.
Liaoning is a Chinese a Type 001 aircraft carrier. The first aircraft carrier commissioned into the People's Liberation Army Navy Surface Force, she is classified as a training ship, intended to allow the Navy to experiment, train and gain familiarity with aircraft carrier operations.
Originally laid down in 1985 for the Soviet Navy as the Kuznetsov-class aircraft cruiser Riga, she was launched on 4 December 1988 and renamed Varyag in 1990. After the dissolution of the Soviet Union in 1991, construction was halted and the ship was put up for sale by Ukraine. The stripped hulk was purchased in 1998 and towed to the Dalian naval shipyard in northeast China.
The ship was rebuilt and commissioned into the People's Liberation Army Navy (PLAN) as Liaoning on 25 September 2012. Its Chinese ship class designation is Type 001. In November 2016, the political commissar of Liaoning, Commodore Li Dongyou, stated that Liaoning was combat ready.
JPTRR
Kostas, Wonderful diorama. Your water is amazing.
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