ALLENTOWN, Pa. – A woman who as a baby just a few hours old was abandoned in the bathroom of a Pennsylvania fast-food restaurant says she has found her birth mother.
Twenty-seven-year-old Katheryn Deprill says she met her biological mother for the first time Monday in an attorney's office. Deprill said Tuesday she felt "pure joy" and it was like "looking in a mirror."
Deprill began her quest March 2 by posting a photo on Facebook. The photo showed Deprill holding up a sign saying, "Looking for my birth mother ... She abandoned me in the Burger King bathroom only hours old, Allentown PA. Please help me find her by sharing my post."
Deprill is known as the Burger King Baby. She says she and her birth mom plan to have more meetings to get to know each other better.


Today Ministers from all over the world gather at the Rabat INDC Forum, jointly organized by the European Commission and the Moroccan government. The Forum aims to assess the adequacy of Intended Nationally Determined Contributions (INDCs) to the Paris agreement.
The EU's negotiating position for the global climate talks in Paris in December, adopted by its Environment Ministers today, calls for climate neutrality - a full phase out of all greenhouse gas emissions. At the same time, it falls short of providing details on the EU's contribution to achieve this.
EU supports a full phase out of emissions, but falls short of action to achieve it.
Today, outside the meeting of Europe's Environment Ministers discussing the EU's negotiating position for global climate talks in Paris in December, climate groups staged a symbolic 'tug-of-war' between polluting fossil fuels and renewable energy. They called for EU governments to join the side of citizens and build a 100% renewable future.
Today's meeting of Pope Francis with EU Environmental Ministers in the Vatican comes at a crucial moment, just two days before an extra Environment Council where EU Member States will adopt the EU's position for the Paris climate summit in December.
Binding national targets have been key to the successful development of renewable energy in the European Union so far, writes Jean-François Fauconnier.
CAN Europe made a submission on the European Commission's Consultation on how to treat land-based emissions in the 2030 target.
CAN Europe is asking for more ambition in the fight against climate change and a 100% renewables target by 2050.
Climate and energy issues are on the agenda of the upcoming G7 Summit in Schloss Elmau, Germany. Chancellor Merkel has reserved the Monday morning (8 June from 0900 to 1030) for discussing the topics, as climate change is one of the key agenda items for her G7 Presidency. In particular, Merkel wants the G7 to contribute to the international climate negotiations in the run up to the UN climate summit in Paris, in December 2015.
The US contribution to the Paris climate agreement, filed within the agreed deadline today, should provide impetus for global efforts to tackle climate change, according to Climate Action Network Europe.
CAN Europe stresses that tackling climate crisis requires more ambition. The new report from the NewClimate Institute shows beyond doubt considerable additional benefits of scaling up climate action.
Europe's leaders botched the opportunity of delivering a clear and forward looking vision on the EU's energy future, argues Wendel Trio.
Last Friday, 6th March, Chairs of the European Parliament Delegations for six* EU accession countries and the Vice-President of the European Parliament sent a letter to Vice-President Maroš Šefčovič and commissioner Miguel Arias Cañete, urging them to ensure that the reform of the Energy Community Treaty align its members with EU membership requirements.
Today the EU's environment ministers signed off the EU's commitment towards the Paris agreement on climate change. According to Climate Action Network Europe and its over 120 members, the EU's announcement is a missed opportunity to set a high benchmark for all the pledges to the global deal.
On Friday, 6th March, the EU's environment ministers will discuss and most likely decide upon the EU's commitment towards the Paris agreement on climate change. On behalf of over 120 members, Climate Action Network Europe highlights the causes of concern regarding attempts to dilute the EU's pledge.
The European Commission's proposals for the EU's contribution to the Paris Climate Summit announced today advocate for a strong, legally binding outcome of the international climate negotiations. On behalf of over 120 organizations, Climate Action Network Europe welcomes the push for the legal form, but calls for more clarity on the amount of greenhouse gasses the EU will emit between 2021 and 2030.
Inclusion of LULUCF in the EU 2030 climate target undermines its environmental integrity.


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text:
Species Profile for Humpback whale (Megaptera novaeangliae)
Listing Status: (and others listed below)
The FWS is currently monitoring the following populations of the Humpback whale
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Arabian Sea DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Cape Verde Island/Northwest Africa
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Western North Pacific
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Central America DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Mexico DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) East Australia DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Gabon/Southwest Africa DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Brazil DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) West Australia DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Hawaii DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Southeast Africa/Madagascar DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Oceania DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) Southeastern Pacific DPS
06/02/1970 National Oceanic and Atmospheric Administration (National Oceanic and Atmospheric Administration) West Indies DPS
01/19/1995 60 FR 3775 3776 Endangered Fish or Wildlife; Special Prohibitions; Approaching Humpback Whales in Hawaiian Waters
11/23/1987 52 FR 44912 44915 NOAA--Approaching Humback Whales in Hawaiian Waters; Interim Rule w/ Request for Comment; 52 FR 44912-44915
11/24/1986 51 FR 42271 42273 NOAA--Proposed Regulations Governing Approaching Humpback Whales in Hawaiian Waters; 51 FR 42271-42273
09/08/2016 81 FR 62259 62320 Identification of 14 Distinct Population Segments of the Humpback Whale (Megaptera novaeangliae) and Revision of Species-Wide Listing; Final Rule
Delisting Final
04/21/2015 80 FR 22303 22356 Identification of 14 Distinct Population Segments of the Humpback Whale (Megaptera novaeangliae) and Proposed Revision of Species-Wide Listing; Proposed Rule
Delisting Proposed
No critical habitat rules have been published for the Humpback whale.
No conservation plans have been created for Humpback whale.

redpajama_set_name: RedPajamaCommonCrawl


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SRC to Implement Phase 2 of New York State UTM Corridor
Startseite/SRC to Implement Phase 2 of New York State UTM Corridor
The Northeast UAS Airspace Integration Research Alliance (NUAIR) has awarded SRC, Inc. a contract to provide surveillance equipment and services for phase two of the implementation of the Initial Operating Capability (IOC) of the Unmanned Secure Autonomous Flight Environment (U-SAFE) corridor project.
Phase two of the U-SAFE program continues the development and implementation of the New York State's Unmanned Aircraft System (UAS) Traffic Management (UTM) Corridor as part of the ongoing Upstate Revitalization Initiative (URI).
This agreement marks a significant step forward in the implementation of the U-SAFE project to enable large-scale, high density, beyond visual line of sight (BVLOS) flight operations of both manned and unmanned aircraft in a defined 50-mile corridor between Rome and Syracuse, New York. SRC's Gryphon Skylight UTM service will provide the airspace surveillance and tracking capabilities necessary to implement a world leading, high volume, UTM corridor.
SRC's Gryphon Skylight system utilizes state-of-the-art, 3-D, active electronically scanned array (AESA) ground radar, passive RF detection and visual identification to allow both manned and unmanned BVLOS UAS traffic to safely coexist in a complex airspace.
"We are excited to be leading the nation in advancing the viability of safe UTM here in Upstate New York," said Paul Tremont, president and CEO of SRC. "Together with NUAIR and New York State, SRC is helping to clear the skies for future UAS operations."
"I am proud that we are getting underway with the final steps of instrumenting our UTM Corridor which will support a number of significant UAS research and operational activities", said Major General (Ret.) Marke "Hoot" Gibson, chief executive officer, NUAIR Alliance. "This instrumented airspace, along with our professional team and dedicated partners, will effectively set a new global standard for UAS development and operations making New York the first choice for test and certification of all classes of UAS."
SRC is looking to fill multiple positions over the next year in the disciplines of digital engineering, systems engineering, software engineering, integration/test, field application support and program management.
Source: Press Release
UAV DACH: Beitrag im Original auf https://www.uasvision.com/2018/09/14/src-to-implement-phase-2-of-new-york-state-utm-corridor/, mit freundlicher Genehmigung von UAS Vision automatisch importiert, Der Beitrag gibt nicht unbedingt die Meinung oder Position des UAV DACH e.V. wieder. Das Original ist in englischer Sprache.
The Editor2018-09-14T06:40:54+02:0014.09.2018|Kategorien: News, UAS Vision, UTM|Tags: News, UAS Vision, UTM|


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Episode 38 Aired: Monday, June 10th 2019
Building a Wine. But Cooler.
Jordan Salcito is the Founder and CEO of DRINK RAMONA, an organic canned wine spritz with a cult-like following. On this episode of In the Sauce, Jordan and Ali talk about recreating a category, making a product that is both quality and accessible and creating a brand that people love.
It's HRN's annual summer fund drive, this is when we turn to our listeners and ask that you make a donation to help ensure a bright future for food radio. Help us keep broadcasting the most thought provoking, entertaining, and educational conversations happening in the world of food and beverage. Become a member today! To celebrate our 10th anniversary, we have brand new member gifts available. So snag your favorite new pizza - themed tee shirt or enamel pin today and show the world how much you love HRN, just go to heritageradionetwork.org/donate
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haven's kitchen
drink ramona
ali cayne
master sommelier
jordan salcito
Building With the "Buyer Buddha"
Wade Yenny is the Director of Grocery for Jimbo's, San Diego's Premier Natural and Organic Food Store, and has over 25 years of experience in all areas of retail including operations, merchandising, procurement, and category management. On this episode of ITS, Wade and Ali talk about the state of...
Building Unfiltered
Jake Karls is Co-Founder of Mid-Day Squares, the fresh, functional chocolate bar that sold over 8 million chocolate bars in its first 3 years. On this episode of ITS, Jake walks Ali through the MDS approach: Part reality TV show, part community, part self-manufacturing. All unabashedly unfiltered...
Building with Target
Ryan Rogers is Ali's former buyer at Target and worked in Produce at the retailer for 14 years. On this episode of ITS, Ryan shares a wealth of knowledge about how emerging brands should pitch and plan to work with Target, how we can set ourselves up for success, and a few insider tips and tricks. A...
Building the Best Idea
Alex Piasecki and Patrick Mateer are the co-founders of Seal the Seasons, the national local foods brand that partners directly with growers to freeze and distribute local produce in more than 30 states and 4000 retail locations across the U.S. On this episode of ITS, Alex and Patrick talk about the...
Building the Rubyverse
Episode 141, Aired: November 24, 2021
Noah Wunsch is the founder of Ruby, a new, super creative line of organic hibiscus water. On this episode of ITS, Noah and Ali talk about the value of going beyond what we've come to think of building a brand identity, how brands need to take time figuring out where can we provide value, and why...
Building the New Convenience Store
Carla Dunham is the CMO of Foxtrot, the company redefining the convenience store with fast delivery, proprietary inventory technology, and the vibe of a neighborhood store. On this episode of ITS, Carla distills 15 years of marketing expertise into a few clear directives for emerging brands. She...
Haven's Kitchen Runs on Oat Milk
Oat milk has a relatively small environmental impact, especially when compared to almond milk, which requires an enormous quantity of...
Navigating the 'Pandemic Pivot' with Teaspressa's Allison DeVane
Allison DeVane is the founder of Teaspressa, a unique product she describes as "coffee inspired tea." It's an all natural...


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Taylor Swift slams 'Shake It Off' copyright lawsuit
August 10, Washington (US): American singer-songwriter Taylor Swift has been accused of copying the words of her popular song "Shake It Off".
A copyright complaint alleging that has received a response from their legal team.
According to Fox News, Swift has been accused of allegedly plagiarizing the words to the 2000 song "Plus Gone Play" by Sean Hall and Nathan Butler.
Now, a new court document says Swift single-handedly composed 2014's "Shake It Off,"
And Swift's attorney accused Hall and Butler of profiting from the song's massive success.
Swift said in a statement that she was only 11 years old when "Plus Gone Play" was published
And she wasn't allowed to watch MTV's "Total Request Live" music video countdown show,
until she was "about 13 years old". According to Fox News.
Swift's mother, Andrea Swift, made a statement in support of this,
In which he claimed that the media was closely monitored at his daughter's house.
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text:
mitochondria uncoupling protein - NCBI
Characteristics of the uncoupling protein from brown-fat mitochondria
Expression of uncoupling protein 3 in mitochondria ... - Springer Link
Apr 10, 2010 - ORIGINAL PAPER. Expression of uncoupling protein 3 in mitochondria protects against stress-induced myocardial injury: a proteomic study.
mitochondria - NCBI
Carboxyatractylate-sensitive uncoupling in liver mitochondria ... - Core
hibernating animals, the maximal respiration rate in the presence of DNP and the rate of the ... ing the rate of uncoupled respiration in the presence of 40 fl DNP,.
Uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3
Induction and degradation of the uncoupling protein ... - NCBI
was degraded much more slowly after noradrenaline removal, with a half-life of ... The prolongation of the half-life of thermogenin after the chronic treatment may.
mitochondria and hepatocytes - NCBI
Isolated Liver Mitochondria - NCBI
maculatum Mitochondria - NCBI
Jun 10, 1985 - King's College, Department ofPlant Sciences, 68 HalfMoon Lane, London SE249JF, United ... trode (Rank Brothers, Cambridge) in medium A (3). ..... TRUESDALE GA, AL DOWNING 1954 Solubility of oxygen in water. Nature.
Mitochondria - NCBI - NIH
ldentification of a Maior Soluble Protein in Mitochondria from ... - NCBI
Physiological Relationships of Uncoupling Protein
Protein Synthesis in Isolated Castor Bean Mitochondria Is ... - NCBI
Cryopreservation of Plant Mitochondria as a Tool for Protein ... - NCBI
Artifactual uncoupling by uncoupling protein 3 in ... - Semantic Scholar
Regulation of Uncoupling Protein-2 and Uncoupling ... - Diabetes
Yadh Khalfallah, Sophie Fages, Martine Laville, Dominique Langin, and ...... Juge-Aubry CE, Hammar E, Siegrist-Kaiser C, Pernin A, Takeshita A, Chin WW,.
of Trypanosoma brucei mitochondria - NCBI
Protein Phosphorylation in Mitochondria from
GAP JUNCTION UNCOUPLING AND DISCONTINUOUS ... - NCBI
Streptozotocin treatment upregulates uncoupling protein 3 expression ...
mitochondrial uncoupling protein 2 axis mediates ... - BioMedSearch
uncoupling protein (mitochondrial proton carrier) - PLOS
Overexpression of Plant Uncoupling Mitochondrial Protein in ...
Abstract. An Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants.
2 downloads 6 Views 1MB Size Report
Jan 26, 1987 - Communicated by J.E.Walker ... phosphate carrier protein (Kolbe and Wohlrab, 1985; Walker et ..... of 15 kd (Thomas and Halestrap, 1981).
The EMBO Journal vol.6 no.5 pp.1367-1373, 1987
Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and the brown fat mitochondria uncoupling protein
Michael J.Runswick, Steven J.Powell, Pal Nyren' and John E.Walker Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK 'Present address: The Department of Biochemistry, The Arrhenius Laboratory, University of Stockholm, Sweden Communicated by J.E.Walker
A cDNA encoding the precursor of the bovine mitochondrial phosphate carrier protein has been cloned from a bovine cDNA library using a mixture of 128 different 17-mer oligonucleotides as hybridisation probe. The protein has an N-terminal extension of 49 amino acids not present in the mature protein. This extension has a net positive charge and is presumed to direct the import of the protein from the cytoplasm to the mitochondrion. Comparison of the protein sequence of the mature phosphate carrier with itself, with ADP/ATP translocase and with the uncoupling protein from brown fat mitochondria shows that all three proteins contain a 3-fold repeated sequence 100 amino acids in length, and that the repeats in the three proteins are related to each other. This inplies that the three proteins have related threedimensional structures and mechaniims and that they share a common evolutionary origin. The distribution of hydrophobic residues in the phosphate carrier protein suggests that each repeated 100 amino acid element is composed of two membrane-spanning a-helices linked by an extensive hydrophilic domain. This model is similar to that first proposed for the ADP/ATP translocase and later for the brown fat mitochondria uncoupling protein. Key words: mitochondria/phosphate carrier/ADP/ATP translocase/cDNA clone -
from both porcine and bovine heart mitochondria (Bisaccia and Palmieri, 1984; Kolbe et al., 1984) and appears on polyacrylamide gels as two bands with similar mol. wts (-33-34 kd) and with the same N- and C-terminal sequences (Kolbe et al., 1984). The N-terminal sequence of the protein has been determined by direct sequence analysis (Kolbe and Wohlrab, 1985; Walker et al., 1986). As described below, we have used this information to design an oligonucleotide which has been used to isolate a cognate cDNA clone from a bovine library (Gay and Walker, 1985a). Its DNA sequence has been determined and the deduced amino acid sequence is related to those of the ADP/ATP carrier and the uncoupling protein from brown fat mitochondria, strongly suggesting that the three carrier proteins have related structures and mechanisms, and are derived from the same ancestral gene.
Results and Discussion Cloning and DNA sequence In the first screening of the cDNA library with the mixture of 128 oligonucleotides, four independent, positively hybridising clones were isolated. However, DNA sequence analysis showed that only one of them contained sequences that could be recognised as coding for the known N-terminal sequence of the phosphate carrier protein (Kolbe and Wohlrab, 1985; Walker et al., 1986). The sequence of the insert in this recombinant was determined completely in both senses of the DNA (see Figure 1 and Materials and methods). Severe difficulties were en0
IRL Press Limited, Oxford, England
=. 1U .1111 BamH l/EcoR
Introduction ATP is synthesised from ADP and inorganic phosphate by oxidative phosphorylation in mitochondria. Supply of substrates in the mitochondrial matrix is maintained by two proteins in the inner membrane, the ADP/ATP translocase and the phosphate carrier (Meijer and van Dam, 1974; La Noue and Schoolwerth, 1979). The former, a protein of Mr 32.8, exchanges external ADP against internal ATP (Klingenberg, 1985). Its amino acid sequence is known (Aquila et al., 1982) and has been shown to contain three repeated related sequences, each of which has been suggested to be folded into a structural element composed of two potential membrane-spanning segments linked by an extramembranous domain (Saraste and Walker, 1982). Related repeated sequences are found also in the uncoupling protein, a protein carrier in the inner membrane of brown fat mitochondria, which returns into mitochondria protons that have been removed by the respiratory chain, and so bypasses ATP-synthase (Aquila et al., 1985). The phosphate carrier mediates the uptake of phosphate into the mitochondrial matrix, either by proton cotransport or in exchange for hydroxyl ions. It has been purified
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Fig. 1. Sequence analysis of a cDNA clone coding for the bovine phosphate carrier protein. The insert is flanked at its 5' end by a BamHI site (denoted by large vertical arrows) and at its 3' end by an EcoRI site (V) and contains two internal EcoRI sites. The sequences of the EcoRI-BamHI fragment (bases 1-579) and the EcoRI fragments (bases 580-627 and 628-1347) were determined with the aid of synthetic oligonucleotides (denoted by heavy bars) after recloning into M13 vectors. The overlaps through the internal EcoRl sites were established with PstI fragments (V, Pst sites) and the sequence of the G:C rich region (bases 140-188) was confirmed with fragments generated by SmaI (small vertical arrows) and Fnu4HI (vertical bars).
M.J.Runswick et al. PstI
GGCCTCTGTGAGCTGCAGCCTTTCTGAGAGACCGGTTGTGTGTTCGCCATCTTAGGGAGAAGATGTACTCGTCCGTGGTGCACCTAGCGCGGGCGAACCCCTTCAACGCGCCTCACCTGC MY S S V V H L A R AN P F N A P H L -49
Q -30
-40 Mature
AGTTGGTGCACGATGGTCTCGCGGGGCCCCGCAGCGACCCCGCCGGGCCCCCGGGCCCACCCCGCCGCTCTCGCAACCTGGCAGCCGcC ECTGTAGAAGAGCAGTgTAGCTGTGACTATG A A A A V E E Q Y S C D Y L
R -20
*R
P -10
GATCTGGCAGATTCTTTATCCTTTGTGGACTTGGAGGAATTATTAGCTGTGGCACAACACATACAGCATTGGTTCCTCTAGATCTGGTTAAATGCAGGATGCAGGTGGACCCACAAAAGT I I P Y S
D LV 40
C R
ACAAGAGCATATTTAATGGATTTTCAGTTACACTCAAAGAGGATGGTTTTCGTGGTTTGGCCAAAGGATGGGCTCCGACTTTCATTGGCTACTCCCTGCAGGGACTCTGCAAGTTTGGCT K
S I
V T 60
E D
F R
|T2
L A 70
K G W A
P T
Eco RI
TTTATGAAGTCTTCAAAGTTTTGTACAGCAACATGCTTGGAGAGGAGAATGCCTATCTGTGGCGCACATCACTGTATTTGGCTGCCTCTGCCAGTGCTGAATTCTTTGCTGACATTGCTC Y E
S N 100
G E E
L W
N AY
S LY
A S
D I AL 130
TGGCTCCTATGGAAGCTGCTAAGGTTCGAATTCAAACCCAGCCAGGTTATGCTAACACTCTGAGGGATGCAGCTCCCAAAATGTATAAGGAAGAAGGCTTAAAGGCGTTCTACAAGGGGG A
R I
A N T
840 760 780 820 740 800 TTGCCCCTCTCTGGATGAGGCAGATACCATACACCATGATGAAGTTTGCCTGCTTTGAACGTACTGTTGAAGCATTGTACAAGTTTGTGGTTCCCAAGCCCCGAAGTGAATGTTCAAAGC A P L W M R Q I P Y TN N K F A C F E R T V E A L Y K F V V P K P R S E C S K P
960 880 900 940 860 920 CAGAGCAGCTGGTTGTCACATTTGTGGCAGGTTACATAGCTGGAGTCTTCTGTGCCATTGTTTCTCACCCTGCTGATTCCGTGGTGTCTGTGTTGAATAAAGAGAAGGGTAGCAGTGCCT E Q L V V T F V A G Y I A G V F C A I V S U P A D S V V S V L N K E K G S S A S 220 230 250 240 1080 980 1020 1000 1040 1060 CTGAGGTCCTGAAGAGGCTTGGATTTAGAGGTGTATGGAAGGGACTCTTTGCCCGTATCATCATGATCGGCACTCTGACTGCACTACAGTGGTTTATCTATGACTCTGTGAAGGTCTACT KR L G F R G V W KG L F A R I I N I G T L T A L Q W F I Y D S V K V Y F E V L 290 260 270 280
Hind III
TCAGGCTCCCTCGCCC'TCCTCCACCTGAGATGCCAGAATCTCTGAAGAAAAAGCTTGGGTACACTCAATAGATAAATGAAAGCAAATATGGACTGAATCTGCGTGTTGATCAGTGTTTGA N P P P P E P E S L K L G Y T Q * R
UGAAAATGCAGAAGGAACTTTTATATATTTGACAGTGTAGGAAGTTGTCTATTCCTAATATAATTACTGTAGTACTCTTGTCAAAAGCAAGAGTTTCAAACTTAATGTGGA
1340 AACTG TACATG AAAAAAAAAAAAAAAA
Fig. 2. DNA sequence of a cDNA encoding the precursor of the bovine mitochondrial phosphate carrier protein. The import sequence of the protein runs from amino acids -49 to -1 and the mature protein from 1-313. The box around the DNA sequence coding for residues 1-6 is the region to which the oligonucleotide probe hybridised. Restriction enzyme sites used in the DNA sequencing and for preparation of prime-cut probes are underlined. The box near to the 3' end of the DNA sequence is a recognition site for addition of poly(A) (Proudfoot and Brownlee, 1976). The dashes under the protein sequence represent protein sequences determined on the mature protein (Kolbe and Wohlrab, 1985; Walker et al., 1986) and on peptides TI -T3 isolated from a tryptic digest of the phosphate carrier protein.
countered in the region comprising bases
140-188, a sequence
composed almost entirely of G and C residues. The final sequence presented in Figure 2 is 1347 bases in length. It is terminated at its 3' end by the sequence (A)16 and so the 3' end of the mRNA is represented in the cDNA clone.
Fourteen bases to the 5' side of this run of A residues is the sequence AATAAA, which presumably serves as a polyadenylation signal (Proudfoot and Brownlee, 1976). By Northern blot 1368
RNA coranalysis (Figure 3) the mRNA in heart poly(A)+ to be 1470 bases responding to the cDNA clone was estimated may long and so the possibility remains that the cDNA clone species of hybridisations weaker not be entirely full length. (Much cannot be ex-
- 3 and 4 kb were also noted, but their presence plained at present.) However, DNA and not RNA standards were
used in this experiment and so the estimates of RNA size are not precise.
Mitochondrial phosphate carrier protein Table I. Amino acid composition of the mature phosphate carrier protein
- 2 -22
l -77
From DNA sequence
Aspartic acid Asparagine Threonine Serine Glutamic acid Glutarnine Proline Glycine Alanine Valine Cysteine Methionine Isoleucine Leucine Tyrosine Phenylalanine Histidine Lysine Arginine Tryptophan
8 5 14 20 18 10 18 25 28 24 8 9 14 29 17 21 2 24 14 5
By amino acid analysisa
14 13 20 27 18 26 29 26 6 8 13 34 16 21 1 19 13 6
0-56 aFrom Kolbe et al. (1984).
of its mol. wt by polyacrylamide gel electrophoresis in the presence of SDS are 33-34 kd, and the mol. wt calculated from the sequence is 35 020. Secondly, the amino acid composition
Fig. 3. Northern blot analysis of poly(A)+ RNA from bovine heart with a probe derived from the cDNA clone for the bovine phosphate carrier protein. Poly(A)+ heart RNA (2.5 1zg) was probed with a prime cut probe corresponding to bases 629-1131 in Figure 2. The marker sizes are determnined with bacteriophage X DNA (3 /Ag) digested with NdeI and endlabelled with [cs-32P]ATP in the presence of the Kienow fragment of DNA polymerase. The sizes of the resulting fragments are shown in kb.
Identification of the clone The DNA sequence contains an open reading frame (bases 210-1148) that encodes the mature phosphate carrier protein. This conclusion is based upon direct protein sequences determined from the intact protein and from peptides derived from it (see Figure 2). These include the N-terminal residues, 1-47, sequenced partly by analysis of the intact protein and partly from a fragment (residues 1-47) produced by treatment of the protein with mild acid (Kolbe and Wohlrab, 1985). It should be noted that residue 48 as determined by DNA sequencing is proline, and that indeed the linkage Asp-47 -Pro-48 would be expected to be acid labile. Sequence analysis (by I.M.Fearnley and J.E.Walker) of peptides TI -T3 (isolated by H.Kolbe and H.Wohlrab) gave further support for the identity of the cDNA. A third piece of evidence is provided by carboxypeptidase digestion of the intact protein (Kolbe et al., 1984) which released firstly amino acids with elution times in an amino acid analyser consistent with the presence of glutamine and threonine (but unresolved from serine and asparagine), and later tyrosine and glycine, and finally leucine. These data fit with the C-terminal sequence LGYTQ deduced from the DNA sequence. Other data measured on the mature phosphate carrier protein are also in accord with the deduced sequence. Firstly, estimates
determined by amino acid analysis for the most part is very similar to that calculated from the sequence; the only significant deviations are in values for leucine and lysine (Table I). The mitochondrial import sequence The open reading frame extends in a 5' direction from the start of the mature protein and encodes a presequence 49 amino acids in length. The mol. wt of the precursor is calculated to be 40 140. The proposed initiator methionine is the only methionine in this region, and the presence of an in-phase stop codon (bases 9-11) excludes the possibility that the reading frame could extend beyond the 5' end of the isolated cDNA. It is assumed that the proposed presequence (amino acids 1-49), as with other nuclearcoded mitochondrial proteins, serves to direct the protein into the mitochondrion and that it is removed during import (Schatz and Butow, 1983). In common with other mitochondrial import sequences, the import sequence of the phosphate carrier has a net positive charge (three histidines, five arginines, two aspartic acids), which has been proposed to help to drive the precursors across the inner mitochondrial membrane, aided by the net negative electrochemical potential inside (Viebrock et al., 1982). The presence of acidic residues in an import sequence is unusual, but not unique; they are found also in the presequences of bovine P1 and P2 proteolipids (Gay and Walker, 1985b), yeast cytochrome cl (Sadler et al., 1984) and the fl-subunit of human F1-ATPase (Ohta and Kagawa, 1986). The length of the presequence is 49 amino acids, which falls within the rather wide range of observed sizes, from 22 amino acids in bovine cytochrome oxidase subunit IV (Lomax et al., 1984) and chicken aspartate aminotransferase (Jaussi et al., 1985) to 68 amino acids in bovine ATP synthase proteolipid P2 (Gay and Walker, 1985b) and yeast cytochrome c peroxidase (Kaput et al., 1982). Other imported mitochondrial proteins, including ADP/ATP translocases from Neurospora crassa (Arends and Sebald, 1984), yeast
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Uncoupler Protein
ADP/ATP
Fig. 4. Comparison of the protein sequences of the phosphate carrier protein with those of bovine ADP/ATP mitochondria uncoupler protein. The calculations were made with the DIAGON (Staden, 1982b) with a score division on the axes represents 10 amino acids.
translocase and the hamster brown fat of 270 and a window of 25 amino acids. One
~ ~ ~ ... ~ ~ ~ ......... ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~
Fr. *
i.r p W.
.!.
%. .(" F)
r..
*~~~~~~~~~.
; !* ,
j,[ !oi ')
;2S
HGFIl rO ^t (O , ; *;HrFt f S fS
.1 U Jg j~$
ii__-__ -_ ___ _
''jH
;.
LJ' F F H' f)
r,> Hi.lr; 1-. i1 P f-
----A ----6 6 - A
Is' P ;P ")
-PHtiV-
U PF
__..L
Fig. 5. Alignment of repeated sequences in the uncoupler protein, ADP/ATP translocase and the phosphate carrier protein. These were made according to Figure 4. * represents a deletion. Identities and conservative substitutions present in at least five of the nine sequences are shaded. Each repeat contains a pair of hydrophobic segments (I and II, III and IV, V and VI respectively), joined by hydrophilic segments, A, B and C as first proposed by Saraste and Walker (1982) for the ADP/ATP translocase.
(Adrian et al., 1986) and cow (S.J.Powell and J.E.Walker, unpublished results), the brown adipose tissue uncoupling proteins from hamster (Aquila et al., 1985) and rat (Bouillard et al., 1986) 1370
and the d subunit of bovine ATP synthase (J.E.Walker and M.J.Runswick, unpublished results) have no processed Nterminal sequence (other than removal of initiator methionine and
Mitochondrial phosphate carrier protein
a)x
after an arginine residue. An arginine residue frequently is found in the vicinity of the cleavage site in import sequences. It may
Uncoupler u
be at the site of cleavage, as for example in bovine cytochrome oxidase subunit IV (Lomax et al., 1984) and ATP synthase P1 and P2 proteolipids (Gay and Walker, 1985b), or up to six amino acids preceding the cleavage site as in human ornithine carbamyl transferase (Horwich et al., 1984).
c L-i
21 ffK t l M I W aJW ISequence repeats A
fl 4 \
IfPairwise comparisons of the phosphate carrier with the bovine -o ADP/ATP translocase and the hamster brown fat mitochondria Ir - 2( uncoupling protein and comparisons of each of the three pro-I-A FL c{ i[]Lteins with itself, are very distinctive (Figure 4). Firstly, they show that the three proteins contain three internal repeats which have -4(0 . been noted previously for the translocase (Saraste and Walker, , ,1982) and for the uncoupling protein (Aquila et al., 1985). 50 l00 150 200.. 250 300 Secondly, the persistence of the parallel diagonal lines 100 amino acids apart across the matrix shows that all of the repeated elements in each of the three proteins are related to each other. O ADP/ATP Translocase This is confirmed by the alignment of the nine segments of se4( quence (Figure 5). The alignment shows that the most highly conserved residues are found in the proposed ca-helical segments (I-VI; see below) and also in the regions of the loops A, B and D. C that connect them to the a-helices. The central parts of loops ~0c A-C are poorly conserved as are the N- and C-terminal regions, H and the regions between the three repeats. It is likely that these A f \l tW W IIJ i regions contribute to the specificities of the carriers. T wV v v v Hydrophobic segments I..9 The similarity between the three proteins is further emphasised l FA-FiiIJ---]II-->B--FiVIJ--1jj-C-V~1 E3 by their hydrophobic profiles (Figure 6). In the case of translocase, this has been suggested to be indicative of the presence of six membrane-spanning segments (I -IV) in the pro-4C tein, although segment II is rather weakly hydrophobic (Saraste 50 100 150 and Walker, 1982). The lengths of the proposed membrane200 250 Residues spanning segments (-25 amino acids) are consistent with the view that they are probably folded into a-helices. These segments contain some charged residues (see span IV of the phosphate carcarrier 4C Phosphate rier). Charged residues in other membrane proteins such as bacteriorhodopsin may be structural, forming ion pairs with equal and opposite charges, or, possibly, as with buried acidic residue in the dicyclohexylcarbodiimide-reactive proteolipid of ATP syna) thase, they may have a function in the activity of the protein. c The regions between segments I and II, HI and IV, and V and VI, called A, B and C respectively (Figures 5 and 6) are relatively hydrophilic and were proposed to form extensive extra-membrane domains. Thus, the 3-fold sequence repeat in the translocase was Ir - go ____ proposed to have a structure comprising a pair of transmembrane a-helices ( and A, HIB and IV, and V and joined by the hydrojI B-----B F --IC philic elements i, and C respectively.Vl The sequence of the uncoupling protein can be interpreted in a similar way, although ... Aquila et al. (1985) preferred a slightly different interpretation 100 150 200 50 250 300 with five transmembrane c-helices and a transmembrane (-sheet, in the region designated as helix II in Figures 5 and 6. The hydrophobic profile of the phosphate carrier suggests that it also Fig. 6. C'omparison of the hydrophobic profiles of the phosphate carrier Iv
------V
-]jf-A-E¶--.
protein w 'ith those of bovine ADP/ATP translocase and the hamster brown fat mitocl hondria uncoupler protein. The calculations were made with
amnino acids. The horizontal bar average hydrophobicity as defined by Kyte and Doolittle
HYDROIPLOT with a window of 11 represent s an (1982).
sometin nes concomitant acetylation of the N terminus); yet the proteinms enter the mitochondria. The site of cleavage of the import sequence of the phosphate
carrier is between two alanine residues and is five amino acids
wilhvsxmeba-pnigsget,adinhscseeg will have SiX membrane-spanning segments, and in this case segment II is somewhat more hydrophobic than in the translocase
and the uncoupling protein. It follows that the phosphate carrier also is constituted of three repeated domains with the same overall organisation as in both ADP/ATP translocase and the uncoupling protein. Common properties of mitochondrial carriers The inner membrane of mitochondria contains at least 12 different carrier proteins involved in the transport of anions and 1371
protons across the membrane (La Noue and Schoolwerth, 1979). Three of them, the ADP/ATP translocase, the uncoupler protein of brown fat mitochondria and the phosphate carrier have been well characterised. All three are single polypeptides with similar mol. wts ( - 33 kd) and related sequences, and it appears in at least the case of the ADP/ATP translocase that the active form is a dimer (Klingenberg, 1985). The a-ketoglutarate/malate carrier also has a similar mol. wt (Bisaccia and Palmieri, 1984) and preliminary sequencing studies indicate that it contains sequences related to the three carriers discussed in this paper (M.J.Runswick, J.E.Walker, F.Bisaccia and F.Palmieri, unpublished results). So it appears that four of the carrier proteins are derived from a common ancestor and is clear that at least three of them are derived by a process involving gene duplications from a smaller ancestral element corresponding to the 100 amino acid residue repeat. However, the pyruvate carrier appears to be smaller and is reported to have an apparent mol. wt of 15 kd (Thomas and Halestrap, 1981). A number of other similarities between carriers have also been noted. For example, both ADP/ATP translocase and the uncoupler protein bind purine nucleotides. However, their binding sites are very different; in the translocase it is mobile and involved in translocation, being accessible from both sides of the membrane. Whereas in the uncoupler protein it is more rigid and is involved in the regulation of translocation (Aquila et al., 1985). These sites have not been adequately mapped in the primary structure of the proteins, nor has the topology of the proteins been established. So the significance of observed structural repeats, which clearly must be of fundamental importance in the functioning of carriers, is obscure.
Materials and methods Oligonucleotide synthesis A mixture of 128 oligonucleotides with the sequence 3' GCNGTNGARGARCARTA 5'was synthesised by T.V.Smith using an Applied Biosystems 380B oligo-
nucleotide synthesiser. This corresponds to the protein sequence AVEEQY, residues 1-6 of the mature protein (Kolbe and Wohlrab, 1985; Walker et al., 1986). Twelve unique oligonucleotides, 16 or 17 bases in length, used as primers in the determination of the complete double-stranded DNA sequence were synthesised by the same procedure. Screening of the cDNA library and DNA sequencing The construction of the library in the pUC8 vector (Vieira and Messing, 1982) and the methods used to screen it with the mixed oligonucleotide were described earlier (Gay and Walker, 1985a,b). Hybridisations were performed for 18-24 h at 5°C below the minimum melting temperature of the mixed oligonucleotide (estimated to be 46°C). The inserted DNA in positively hybridising clones was released by digestion with EcoRI and BamHI. The products were fractionated by electrophoresis in the presence of ethidium bromide in a 1 % LGT agarose minigel. Bands were excised from the gel under u.v. light. DNA was recovered by phenol extraction (Wieslander et al., 1979) and cloned into appropriate M13mp8 and nmp9 vectors (Messing and Vieira, 1982). Sequences at the ends of the cloned fragments were determined by primed synthesis in the presence of chain terminators (Sanger et al., 1977; Biggin et al., 1983) with the universal primer, LMB2 (Duckworth et
One of the four recombinants that hybridised with the oligonucleotide probe was found to contain sequences encoding the N-terminal region of the phosphate carrier protein. The insert in this recombinant had been released from the plasmid by digestion with EcoRI and BamHI. Two fragments were detected by ethidium bromide staining after agarose gel electrophoresis: an EcoRI -BamHI fragment (bases 1-579, Figure 2) and an EcoRI fragment (bases 628-1347, Figure 2) estimated to be 600 and 730 bp respectively. The sequences of the inserts were completed by synthesising appropriate oligonucleotides for use as primers in further sequencing experiments. After synthesis their concentrations were estimated by measurement of OD260. They were diluted in H20 at 0.2 pmol/,ul, and 2 ul of solution of the oligonucleotide was used in each sequencing reaction. In order to overlap these two sequences, a PstI restriction enzyme digest was investigated (see Figures 1 and 2 for restriction enzyme sites). Thereby it was found that the two original fragments were joined by a small EcoRI
fragment (bases 579-628) that had not been detected by ethidium bromide staining after agarose gel electrophoresis. This small fragment was also cloned and sequenced. Compressions in sequencing experiments were resolved by substitution of deoxy-inosine triphosphate for dGTP in the sequencing mixtures, the products being separated by electrophoresis in a 6% gel (Mills and Kramer, 1979; Bankier and Barrell, 1983). Further confirmation of compressed sequences was obtained by cutting directly in these sequences with SmaI and Fnu4HI, and cloning and sequencing the resultant products. Computer methods DNA sequences were compiled into a data base with aid of the computer programs DBAUTO and DBUTIL (Staden, 1982a) and then analysed with ANALYSEQ (Staden, 1984). Protein sequences were examined with HYDROPLOT, a version of SOAP (Kyte and Doolittle, 1982) and DIAGON (Staden, 1982b). Northern analysis Total heart mRNA was extracted from fresh bovine heart tissue by the method of Chirgwin et al. (1979). As soon as possible after slaughter of the animal, cubes of tissue were frozen in liquid nitrogen, transported to the laboratory, and then pulverised under liquid nitrogen and homogenised for 60 s with a Polytron homogeniser in 4 M guanidinium thiocyanate containing 25 mM sodium citrate, pH 7.0, 0.1 M 2-mercaptoethanol and 0.5% sarkosyl. The homogenate was centrifuged (130 000 g, 18 h, 20°C) through a cushion of 5.7 M CsCl containing 0.1 M EDTA, pH 7.0. Poly(A)+ RNA was prepared by the method of Aviv and Leder (1972) except that NaCl was replaced by LiCl (Maniatis et al., 1982). Northern blot analysis was performed as follows. RNA samples were treated with glyoxal (Carmichael and McMaster, 1980), and fractionated on a 1.4% HGT agarose gel submerged in stirred 10 mM sodium phosphate, pH 7.0. RNA was transferred to Hybond N (Amersham International PLC, Amersham, UK) and cross-linked to the matrix by u.v. irradiation. Hybridisation was performed at 65°C with a 'prime cut' probe (Farrell et al., 1983) corresponding to the EcoRI-HindHl fragment (bases 629-1131, see Figure 2). Membranes were washed at 65°C four times in 6 x SSC, twice in 2 x SSC and twice in 0.2 x SSC (SSC is 0.15 M NaCl, 0.015 M Na citrate). Autoradiography was carried out for 3 days at -70°C with preflashed film and an intensifying screen.
Acknowledgements We thank Drs Kolbe and Wohlrab for sending peptides T1-T3 for sequence analysis, and Mr I.M.Fearnley for his help with these experiments. We are grateful to Mr T.V.Smith for synthesising oligonucleotides. P.N. was supported by a postdoctoral scholarship from the N.F.R.
References Adrian,G.S., McCammon,M.T., Montgomery,D.L. and Douglas,M.G. (1986) Mol. Cell Biol., 6, 626-634.
Aquila,H., Misra,D., Eulitz,M. and Klingenberg,M. (1982) Hoppe-Seyler's Z. Physiol. Chem., 363, 345-349. Aquila,H., Link,T.A. and Klingenberg,M. (1985) EMBO J., 4, 2369-2376. Arends,H. and Sebald,W. (1984) EMBO J., 3, 377-382. Aviv,H. and Leder,P. (1972) Proc. Natl. Acad. Sci. USA, 69, 1408-1412. Bankier,A.T. and Barrell,B.G. (1983) In Flavell,R.A. (ed.), Techniques in Nucleic Acid Biochemistry. Elsevier, Ireland, B508/1 - B508/31. Biggin,M.D., Gibson,T.J. and Hong,G.F. (1983) Proc. Natl. Acad. Sci. USA, 80, 3963-3965. Bisaccia,F. and Palmieri,F. (1984) Biochim. Biophys. Acta, 766, 386-394. Bouillard,F., Weissenbach,J. and Riquier,D. (1986) J. Biol. Chem., 261, 1487-1490.
Carmichael,G.G. and McMaster,G.K. (1980) Methods Enzymol., 65, 380-391. Chirgwin,J.M., Przbyla,A.F., MacDonald,R.J. and Rutter,W.J. (1979) Biochemistry, 18, 5294-5299. Duckworth,M.L., Gait,M.J., Goelet,P., Hong,G.-F., Singh,M. and Titmas,R.C. (1981) Nucleic Acids Res., 9, 1691-1706. Farrell,P.J., Deininger,P.L., Bankier,A. and Barrell,B.G. (1983) Proc. Natl. Acad. Sci. USA, 80, 1565-1569. Gay,N.J. and Walker,J.E. (1985a) Biochem. J., 225, 707-712. Gay,N.J. and Walker,J.E. (1985b) EMBO J., 4, 3519-3524. Horwich,A.L., Fenton,W.A., Williams,K.R., Kalonsek,F., Krans,J.P., Doolittle,R.F., Konigsberg,W. and Rosenberg,L.E. (1984) Science, 224, 1068-1074. Jaussi,R., Cotton,B., Juretic,P.C. and Schumperli,D. (1985) J. Biol. Chem., 260, 16060-16063.
Kaput,J., Goltz,S. and Blobel,G. (1982) J. Biol. Chem., 257, 15054-15058. Klingenberg,M. (1985) In Martonosi,A.N. (ed.), The Enzymes of Biological Membranes. Plenum Press, Vol. 4, pp. 511-553. Kolbe,H.V.J., Costello,D., Wong,A., Lu,R.C. and Wohlrab,H. (1984) J. Biol. Chem., 259, 9115-9120.
Mitochondrial phosphate carrier protein Kolbe,H.V.J. and Wohlrab,H. (1985) J. Biol. Chem., 260, 15899-15906. Kyte,J. and Doolittle,R.F. (1982) J. Mol. Biol., 157, 105-132. La Noue,K.F. and Schoolwerth,A.C. (1979) Annu. Rev. Biochem., 48, 871-922. Lomax,M.I., Bachman,N.J., Nasoff,M.S., Caruthers,M.H. and Grossman,L.I. (1984) Proc. Natl. Acad. Sci. USA, 81, 6295-6299. Maniatis,T., Fritsch,E.F. and Sambrook,J. (1982) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York. Meijer,A.J. and van Dam,K. (1974) Biochim. Biophys. Acta, 346, 213-244. Messing,J. and Vieira,J. (1982) Gene, 19, 269-276. Mills,D.R. and Kramer,F.R. (1979) Proc. Natl. Acad. Sci. USA, 76, 2232-2235. Ohta,S. and Kagawa,Y. (1986) J. Biochem., 99, 135 -141. Proudfoot,N.J. and Brownlee,G.G. (1976) Nature, 263, 211-214. Sadler,I., Suda,K., Schatz,G., Kaudewitz,F. and Haid,A. (1984) EMBO J., 3, 2137-2143. Sanger,F., Nicklen,S. and Coulson,A.R. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467. Saraste,M. and Walker,J.E. (1982) FEBS Lett., 144, 250-254. Schatz,G. and Butow,R.A. (1983) Cell, 32, 316-318. Staden,R. (1982a) Nucleic Acids Res., 10, 4731-4751. Staden,R. (1982b) Nucleic Acids Res., 10, 2951-2961. Staden,R. (1984) Nucleic Acids Res., 12, 521-538. Thomas,A.P. and Halestrap,A.P. (1981) Biochem. J., 1%, 471-479. Viebrock,A., Perz,A. and Sebald,W. (1982) EMBO J., 5, 565-571. Vieira,J. and Messing,J. (1982) Gene, 19, 259-268. Walker,J.E., Feamley,I.M. and Blows,R.A. (1986) Biochem. J., 237, 73-84. Wieslander,L. (1979) Anal. Biochem., 98, 305-309. Received on November 24, 1986; revised on January 26, 1987
Note added in proof Since this paper was submitted, Aquila et al. [FEBS Lett., 212, 1-9 (1987)] have published a review containing a partial protein sequence for the bovine phosphate carrier. Residues 226-255 and 270-271, corresponding to a large segment of the third repeat, have not been determined. They find aspartic acid and glutamine at residues 87 and 107 respectively, whereas we find cysteine and asparagine at these positions.
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Home / PS4 Store / PS4 Games / Minecraft: Story Mode – Season Disc – PlayStation 4
Minecraft: Story Mode – Season Disc – PlayStation 4
£29.99 (as of November 27, 2015, 5:59 pm) £29.48
Created by award-winning adventure game powerhouse Telltale Games, in partnership with Minecraft creators, Mojang
Featuring the voices of Patton Oswalt (Ratatouille, Agents of S.H.I.E.L.D), Brian Posehn (The Sarah Silverman Program, Mission Hill), Ashley Johnson (The Last of Us, Tales from the Borderlands), Scott Porter (Friday Night Lights, X-Men)
Dave Fennoy (The Walking Dead: A Telltale Games Series, Batman: Arkham Knight), Martha Plimpton (The Goonies, Raising Hope), Corey Feldman (The Goonies, Stand by Me), Billy West (Futurama, Adventure Time), Paul Reubens (Tron: Uprising, Pee-Wee's Playhouse)
Category: PS4 Games
As Jesse, you'll embark on a perilous adventure across the Over world, through the Nether, to the End, and beyond. You and your friends revere the legendary Order of the Stone: Warrior, Redstone Engineer, Griefer, and Architect; slayers of the Ender Dragon. While at EnderCon in hopes of meeting Gabriel the Warrior, you and your friends discover that something is wrong. something dreadful. Terror is unleashed, and you must set out on a quest to find The Order of the Stone if you are to save your world from oblivion. A five-part episodic series with the disc including episode one, and grants access to four more episodes as they become available to download (Internet connection required).
You will drive the story through the decisions you make: what you say to people (and how you say it), and what you choose to do in moments of thrilling action will make this YOUR story
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strength and resilience of our people. here i want to speak directly to the american people. what happened in paris is truly horrific. i understand that people worry that something similar to happen here. i want you to know that we will continue to do everything in our power to defend our nation. since 9/11 we have taken extraordinary measures to strengthen our homeland security. our counter terrorism, homeland security and law enforcement professionals, federal state and local they are tireless, they have prevented attacks and they have saved lives, they are working every hour every day for our security. they did so before paris, they do so now and they will not stop.
allow me first and foremost to thank the president of the united states, barack obama, the solidity he has shown immediately after the attacks, he was the first one to call me, it was very late in france, 2:00 a.m. when he called, the president of the united states. i would like to express his solidity to france his emotion and compassion against the horror and on that night he meant to tell me that the united states stood by france. that the help that could be provided to france would have no limit and that we had a duty, a joined duty to put our forces together and fight terrorism.


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Posted inCalgary Votes 2017
Majority of controversial Students Count group elected
by Amy Simpson October 19, 2017 September 4, 2020
Five individuals ran in the school board trustee election as part of a slate they called Students Count, and despite controversy, three have been elected.
The Students Count group is advocating for more classroom funding, improving math marks, changing report card marking schemes to percentages, establishing an independent ombudsman to ensure student wellness and removing the ability for campaigns to take in corporate and union donations.
The group consisted of Althea Adams (Ward 3 and 4), Lisa Davis (Ward 6 and 7), Sabrina Bartlett (Ward 8 and 9), Mike Bradshaw (Ward 12 and 14) and Sadiq Valliani (Ward 11 and 13). Adams, Davis and Bradshaw were elected, with Barlett only losing by a little under 2,000 votes.
None of the elected members responded to our request for an interview.
Althea Adams, a member of Students Count, has been elected as the school trustee for Ward 3 and 4. Photo courtesy of the Calgary Herald.
The other trustees elected were Trina Hurdman (Ward 1 & 2), Marilyn Dennis (Ward 5 & 10), Richard Hehr (Ward 8 & 9) and Julie Hrglicka (Ward 11 & 13).
Some voiced concerns when they heard the group was running as a coalition.
In an interview with the Calgary Herald before the election, MRU political science professor Lori Williams said running as a group could be a weakness.
"Slates often tend to look too much like parties, and voters like a more direct connection to issues, especially in local elections where voters want individual candidates to be connected to local issues."
However, Williams also suggested that candidates running together as a group could just mean there are overarching frustrations with the system.
Jodi Nickel, chair and associate professor of Mount Royal's Department of Education, said she was concerned when she heard a group was running.Lisa Davis, a member of Students Count, has been elected as the school trustee for Ward 6 and 7. Photo courtesy of the Calgary Herald
"I think it is really important that the trustees aren't just looking for political power and pushing an agenda, but that they actually care about education and have something to contribute to society," she said.
The group was endorsed on Twitter by UCP leadership candidate Jason Kenney, but they denied any official links to his campaign.
Although most Calgarians focus their attention largely on the mayoral and councillor races, Nickel said people should also care about the trustees.
"Education is a public trust and the strength of a society really depends largely on how the citizenry is educated," she said.
In Calgary's municipal election, while voting for their mayor and city council, citizens also vote on the governing body of either the Calgary Board of Education or the Calgary Catholic School District.Mike Bradshaw, a member of Students Count, has been elected as the school trustee for Ward 12 and 14. Photo courtesy of the Calgary Herald
But what does this Board of Trustees even do?
"They are the ones that make sure that the school does their job well and that tax dollars are used well," said Nickel.
Often, trustees deal with difficult decisions around funding.
"If you have a school with only 100 students in it, as much as it is disappointing for parents to see their neighbourhood school close down, that's the more fiscally responsible thing to do that will benefit ultimately more of the schools."
A list of the board's specific roles can be found here.
However, although 387,306 people voted in the general election, only 232,069 people voted for a public school board trustee and 77,795 people voted for an independant school board trustee.
Nickel said there are a number of factors that could be contributing to the low voter apathy.
"I guess people do see the stakes at being fairly low," she said.
"There are enough checks and balances in the system that even if a weak trustee was elected that probably the system is going to keep rolling."
She also suggested Alberta's good educational system could also lead to voter apathy.
"Canada scores high, but if you isolate Alberta results, we score really really high," Nickel said speaking of the Programme for International Student Assessment.
"I think people probably have this confidence that we have a great education system and so we are not too worried, where if there was real problems, maybe they would take more of an interest."
asimpson@cjournal.ca
Editor: Jolene Rudisuela | jrudisuela@cjournal.ca
Opinion: How my childhood love for slang will make me a better writer
Proposed bylaw could bring an end to shisha bars
Farkas calls out council's pay cheques and city's lack of transparency


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Kevin T. Abbott
Peoria, Illinois Office
Kevin Abbott defends healthcare professionals facing claims of medical malpractice. He also works with both hospitals and insurers to develop policy and procedure language and ensure compliance with the Peer Review and Medical Studies Act and HIPPA. Kevin has also advised his clients on third-party subpoena issues.
Kevin's clients also benefit from his extensive litigation experience. He handles all aspects of the process from preparing witnesses and taking and defending depositions, to motions and trial. Over the course of his career, Kevin has secured several summary judgment victories for hospitals on issues involving liability, causation, and agency.
Prior to joining Hinshaw, Kevin practiced for over six years at a medical malpractice firm in Rockford, Illinois. He also gained valuable experience working as a law clerk with two accomplished Chicago-based plaintiff's medical malpractice firms.
While attending law school, Kevin took advantage of extern opportunities in Chicago and spent a summer as an intern for the American Medical Association and their Counsel on Ethical and Judicial Affairs. As a student, he was also a member of the DePaul Law Review.
Recognized as a "Rising Star" by Illinois Super Lawyers magazine, 2019
CALI Award for the highest grade in Commercial Arbitration; Corporate Finance; and Legal Profession
Medical Malpractice Litigation
Second chaired a two-week jury trial involving claims that a physician improperly performed a Roux-en-Y Gastric Bypass and improperly managed the patient's post-operative course, leading to his death. Plaintiff sought more than $9 million. After eight days, the jury returned a verdict in favor of all defendants.
Co-tried a birth injury case involving claims of brachial plexus palsy and cognitive injuries. The case required the defense of a hospital, its employed nurses, and an apparent agency claim against an obstetrician. A verdict was secured for the defense.
Like most Midwesterners, Kevin enjoys hockey. When weather permits, he likes golfing, canoeing, and hiking. Kevin also enjoys reading, cooking, and traveling.
J.D., summa cum laude, DePaul University College of Law, 2011
B.A., University of Illinois at Urbana-Champaign, 2007


Congratulations to the winners of the Sponsor Selfie Challenge!!!
There were so many great submissions in the Sponsor Selfie Challege! We are so blessed at Circle of Hope to have such dedicated sponsors.
Thank you for supporting and loving the children of Malawi!
First Place winners are Hayley, MicKenzie, and Carson!
Second Place winner is Carmen!
Third Place winner is Daphne!
Not sponsoring a child yet? Pick one out today!
Posted on December 4, 2014 by Karen Roller.


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SunSpec Alliance and XBRL US Announce Public Review of Orange Button Solar Data Standards
Posted on Tuesday, September 12, 2017
Investors, developers, software providers are encouraged to participate
SunSpec Alliance and XBRL US announced today the launch of a 30-day public review period for the initial draft of Orange Button data standards for solar financing. The digital dictionary of terms, called a taxonomy, was developed as part of the U.S. Department of Energy SunShot Initiative's Orange ButtonSM program (https://energy.gov/sunshot) that aims to make it easier to aggregate and share solar data, facilitating more efficient and cost-effective financing of distributed energy projects. SunSpec Alliance heads the Orange Button project team that is tasked with establishing an open, easy-to-adopt, solar data architecture, and standards.
"Standardizing data collection is a critical means to reduce the soft costs of solar financing," said Tom Tansy, Chairman of SunSpec Alliance, "All solar energy stakeholders owe it to themselves and their businesses to review the standards and give us feedback. Every participant has a chance to influence the final standards that will be the industry norm in solar financing. That will benefit participants' companies, and ultimately, the entire industry."
The XBRL data standard was chosen for Orange Button because it is a free, open, global standard, uniquely suited to financial data. XBRL US is a nonprofit standards developer that built XBRL data standards for public companies, mutual funds, and credit rating agencies, under contract with the U.S. Securities and Exchange Commission. XBRL US leads the effort to build the financial portion of Orange Button standards, leveraging elements from the U.S. GAAP Taxonomy for public companies, and from existing solar data standards.
Public companies in the solar industry, such as electric utilities, investors, and solar developers, already format their financials in XBRL which will ease the path to Orange Button adoption. The value of standards increases as more companies use them. A growing number of companies and applications are adopting, implementing, and facilitating this initiative, such as Sunnova Energy Corporation and Wells Fargo, among others. In Oracle's Enterprise Performance Reporting Cloud Service, the Disclosure Management capability enables the creation of XBRL files based on the Orange Button taxonomy.
"XBRL is the ideal standard for Orange Button given the need for consistent, comparable financial information in solar financing," said Campbell Pryde, President and CEO, XBRL US, "XBRL US, along with SunSpec, has a strong interest in the success of Orange Button, and we look forward to continuing to develop, refine and expand on the taxonomy to cover more use cases, and to continue to meet the needs of the industry as they evolve."
Orange Button standards represent the life cycle phases of solar financing, including feasibility, design, construction, finance and operations. Content available during the public review includes the digital dictionary of data fields and definitions, which can be easily viewed, and commented on, through a review tool called Yeti, contributed to the Orange Button program by CoreFiling, a global supplier of XBRL-enabled financial reporting software.
To access the public review, go to: https://xbrl.us/events/sunspec-201710113/
About the SunShot Initiative
The U.S. Department of Energy SunShot Initiative is a national effort to drive down the cost of solar electricity and support solar adoption. SunShot aims to make solar energy a low-cost electricity source for all Americans through research and development efforts in collaboration with public and private partners. Learn more at energy.gov/sunshot.
About the SunSpec Alliance
The SunSpec Alliance is a trade alliance of developers, manufacturers, researchers and service providers, together pursuing information and communications standards for the distributed energy industry. SunSpec standards address most operational aspects of PV, energy storage and other distributed energy power plants on the smart grid—including residential, commercial, and utility-scale systems—thus reducing cost, promoting innovation, and accelerating industry growth. More than 100 organizations are members of the SunSpec Alliance, including global leaders from Asia, Europe, and North America. Membership is open to corporations, non-profits, labs, governments and educational institutions. For more information about the SunSpec Alliance, or to download SunSpec specifications free of charge, please visit www.sunspec.org.
XBRL US is the non-profit consortium for XBRL business reporting standards in the U.S. and represents the business information supply chain. Its mission is to support the implementation of business reporting standards through the development of taxonomies for use by U.S. public and private sectors, with a goal of interoperability between sectors, and by promoting XBRL adoption through marketplace collaboration. XBRL US has developed taxonomies for U.S. GAAP, credit rating and mutual fund reporting under contract with the U.S. Securities and Exchange Commission, and has developed industry-specific taxonomies for corporate actions, solar financing and surety processing.
Tom Tansy
tom@sunspec.org
Michelle Savage
XBRL US
michelle.savage@xbrl.us
software development solar
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77 Facts About Morocco
The Marinid and Saadi dynasties otherwise resisted foreign domination, and Morocco was the only North African nation to escape Ottoman dominion.
Morocco is a unitary semi-constitutional monarchy with an elected parliament.
Fun Fact Morocco
United Nations Interesting Fact
Barbary lion Fact
Fact About Spain
Baghdad Trivia
Morocco Interesting Fact
Today, Morocco occupies two-thirds of the territory, and efforts to resolve the dispute have thus far failed to break the political deadlock.
In Turkish, Morocco is known as, a name derived from its ancient capital of Fes.
The English name Morocco is an anglicisation of the Spanish name for the country,.
North African Marinid Carthage Baghdad Abbasid Almoravid dynasty Mediterranean Maghreb Almohad Ottoman Empire Spain United States France Western Sahara United Nations Mediterranean Sea Atlantic Ocean Gibraltar North Atlantic Ocean Algeria California Portugal
Morocco later became a realm of the Northwest African civilisation of ancient Carthage, and part of the Carthaginian empire.
The first independent Muslim state in the area of modern Morocco was the Kingdom of Nekor, an emirate in the Rif Mountains.
Morocco convinced the Awraba Berber tribes to break their allegiance to the distant Abbasid caliphs in Baghdad and he founded the Idrisid dynasty in 788.
The Idrisids established Fes as their capital and Morocco became a centre of Muslim learning and a major regional power.
Under the Sanhaja Almoravid dynasty and the Masmuda Almohad dynasty, Morocco dominated the Maghreb, al-Andalus in Iberia, and the western Mediterranean region.
Morocco was faced aggression from Spain and the Ottoman Empire allies pressing westward.
Morocco was the first nation to recognise the fledgling United States as an independent nation in 1777.
In March 1956 the French protectorate was ended and Morocco regained its independence from France as the "Kingdom of Morocco".
In 1984, Morocco left the Organisation of African Unity in protest at the SADR's admission to the body.
Morocco is a cautious moderniser who has introduced some economic and social liberalisation.
Morocco unveiled an autonomy blueprint for Western Sahara to the United Nations in 2007.
Morocco was the first Spanish leader in 25 years to make an official visit to the territories.
Morocco has a coast by the Atlantic Ocean that reaches past the Strait of Gibraltar into the Mediterranean Sea.
Morocco is a Northern African country, bordering the North Atlantic Ocean and the Mediterranean Sea, between Algeria and the annexed Western Sahara.
In terms of area, Morocco is comprised predominantly of "hot summer Mediterranean climate" and "hot desert climate" (BWh) zones.
The southeasternmost portions of Morocco are very hot, and include portions of the Sahara Desert, where vast swathes of sand dunes and rocky plains are dotted with lush oases.
Countries or regions that share the same climatic characteristics with Morocco are Portugal, Spain and Algeria and the U S state of California.
The avifauna of Morocco includes a total of 454 species, five of which have been introduced by humans, and 156 are rarely or accidentally seen.
Barbary lion, hunted to extinction in the wild, was a subspecies native to Morocco and is a national emblem.
Morocco is home to six terrestrial ecoregions: Mediterranean conifer and mixed forests, Mediterranean High Atlas juniper steppe, Mediterranean acacia-argania dry woodlands and succulent thickets, Mediterranean dry woodlands and steppe, Mediterranean woodlands and forests, and North Saharan steppe and woodlands.
Barbary lion Democracy Non-Aligned Movement African Union Arab League Islamic George W Bush Trump administration Israel GDP South Africa Casablanca Europe Science Mohammed EMI Arabic Jewish Eurovision Bread FIFA Germany
Morocco was an authoritarian regime according to the Democracy Index of 2014.
Constitution of Morocco provides for a monarchy with a Parliament and an independent judiciary.
Morocco presides over the Council of Ministers; appoints the Prime Minister from the political party that has won the most seats in the parliamentary elections, and on recommendations from the latter, appoints the members of the government.
The Assembly of Representatives of Morocco has 325 members elected for a five-year term, 295 elected in multi-seat constituencies and 30 in national lists consisting only of women.
Morocco is a member of the United Nations and belongs to the African Union, Arab League, Arab Maghreb Union (UMA), Organisation of Islamic Cooperation (OIC), the Non-Aligned Movement and the Community of Sahel–Saharan States (CEN_SAD).
Morocco's relationships vary greatly between African, Arab, and Western states.
Morocco has had strong ties to the West in order to gain economic and political benefits.
Morocco was the only African state not to be a member of the African Union due to its unilateral withdrawal on 12 November 1984 over the admission of the Sahrawi Arab Democratic Republic in 1982 by the African Union as a full member without the organisation of a referendum of self-determination in the disputed territory of Western Sahara.
Morocco was given the status of major non-NATO ally by the George W Bush administration in 2004.
Morocco was the first country in the world to recognise US sovereignty.
Morocco is included in the European Union's European Neighbourhood Policy which aims at bringing the EU and its neighbours closer.
In 2020, the United States under the Trump administration became the first Western country to back Morocco's contested sovereignty over the disputed Western Sahara region, on the agreement that Morocco would simultaneously normalize relations with Israel.
In 2006, the government of Morocco suggested autonomous status for the region, through the Moroccan Royal Advisory Council for Saharan Affairs.
Morocco is officially divided into 12 regions, which, in turn, are subdivided into 62 provinces and 13 prefectures.
Morocco has been accused of detaining Sahrawi pro-independence activists as prisoners of conscience.
Morocco's economy is considered a relatively liberal economy governed by the law of supply and demand.
Morocco has become a major player in African economic affairs, and is the fifth largest economy in Africa by GDP.
Morocco was ranked as the first African country by the Economist Intelligence Unit's quality-of-life index, ahead of South Africa.
Morocco is relatively inexpensive because of the devaluation of the dirham and the increase of hotel prices in Spain.
Morocco has an excellent road and rail infrastructure that links the major cities and tourist destinations with ports and cities with international airports.
Morocco produces enough food for domestic consumption except for grains, sugar, coffee and tea.
In 2014, Morocco began the construction of the first high-speed railway system in Africa linking the cities of Tangiers and Casablanca.
Morocco has the largest port in Africa and the Mediterranean called Tanger-Med, which is ranked the 18th in the world with a handling capacity of over 9 million containers.
Morocco has embarked upon the construction of large solar energy farms to lessen dependence on fossil fuels, and to eventually export electricity to Europe.
Morocco announced that investment in science and technology would rise from US$620, 000 in 2008 to US$8.
Morocco was ranked 77th in the Global Innovation Index in 2021, down from 74th in 2019.
In 2012, the Hassan II Academy of Science and Technology identified a number of sectors where Morocco has a comparative advantage and skilled human capital, including mining, fisheries, food chemistry and new technologies.
Morocco has a large diaspora, most of which is located in France, which has reportedly over one million Moroccans of up to the third generation.
Morocco has more than four dozen universities, institutes of higher learning, and polytechnics dispersed at urban centres throughout the country.
Morocco has some of prestigious postgraduate schools, including: Mohammed VI Polytechnic University, l'Institut National des Postes et Telecommunication, Ecole Nationale Superieure d'Electricite et de Mecanique (ENSEM), EMI, ISCAE, INSEA, National School of Mineral Industry, Ecole Hassania des Travaux Publics, Les Ecoles nationales de commerce et de gestion, Ecole superieure de technologie de Casablanca.
Morocco is a developing country that has made many strides to improve these categories.
In 2005, The government of Morocco approved two reforms to expand health insurance coverage.
In 2014, Morocco adopted a national plan to increase progress on maternal and child health.
Morocco has made significant progress in reducing deaths among both children and mothers.
In 2017, Morocco ranked 16th out of 29 countries on the Global Youth Wellbeing Index.
Morocco is a country with a rich culture and civilisation.
Morocco has set among its top priorities the protection of its diverse legacy and the preservation of its cultural heritage.
Culturally speaking, Morocco has always been successful in combining its Berber, Jewish and Arabic cultural heritage with external influences such as the French and the Spanish and, during the last decades, the Anglo-American lifestyles.
Under the Almohad dynasty Morocco experienced a period of prosperity and brilliance of learning.
Morocco founded a great library, which was eventually carried to the Casbah and turned into a public library.
Morocco is home to Andalusian classical music that is found throughout Northwest Africa.
Morocco participated in the 1980 Eurovision Song Contest, where it finished in the penultimate position.
Cinema in Morocco has a long history, stretching back over a century to the filming of Le chevrier Marocain by Louis Lumiere in 1897.
The cuisine of Morocco is mainly a fusion of Moorish, European and Mediterranean cuisines.
The most commonly eaten red meat in Morocco is beef; lamb is preferred but is relatively expensive.
Bread in Morocco is principally from durum wheat semolina known as khobz.
Bakeries are very common throughout Morocco and fresh bread is a staple in every city, town and village.
In 1986, Morocco became the first Arab and African country to qualify for the second round of the FIFA World Cup.
Morocco was scheduled to host the 2015 Africa Cup of Nations, but refused to host the tournament on the scheduled dates because of fears over the ebola outbreak on the continent.
Morocco made five attempts to host the FIFA World Cup but lost five times to the United States, France, Germany, South Africa and a Canada–Mexico–United States joint bid.
Spectator sports in Morocco traditionally centered on the art of horsemanship until European sports—football, polo, swimming, and tennis—were introduced at the end of the 19th century.
Morocco was one of the continent's pioneers in basketball as it established one of Africa's first competitive leagues.


BALIF is hosting a South Bay Happy Hour for our members at Stanford and Santa Clara. Both student and attorney members are encouraged to attend. Refreshments will be provided, and we have a private area reserved for our guests, so bring a friend (or two). Cheers!


1. NEW YEEZY BOOST 350 V2 carry woven PRIMEKNIT shoes decorated with distinctive unique vehicle design and in the middle of shoe collar button.
2. the first translucent rubber outsole, and TPU shoes side structure with spectacular visual effects, as well asExcellent traction.
3. In addition, YEEZY adidas brand new BOOST 350 V2 applied technology, create, Shock-proof and return strong outsole structure.


Newsgur USA: Exactly what is expected from Diaz-Canel?
Exactly what is expected from Diaz-Canel?
During his political journey, the newly appointed Cuban president, Miguel Mario Díaz-Canel, has prospered without running, without heterodox initiatives because it was not necessary, his destiny was marked. He never strayed from ideological dogmas, nor are he known for reckless reformist proposals.
During the elections for deputies of the National Assembly last month, Díaz-Canel paid tribute to the historical generation that has led and that forged the revolution. Diaz-Canel's speech has been dogmatic, predictable. Barely involved in political activism, he has not hesitated to keep up with the digital ones he considers hostile, trained by counterrevolutionary, social democratic tools, which in the nomenclature of the party is similar.
The certain thing is that the new head of government, with the accompaniment of Raul Castro in the general secretary of the PCC, will keep blocked the political opening, and they will extinguish any attempt to force it.
All the governments and societies that demand a democratic transition in Cuba, would like the new president, Miguel Díaz-Canel, to be a renegade, to swear falsely. The entire European Union and the majority of the UN General Assembly would applaud the 57-year-old engineer who replaces Raúl Castro, promised to respect the fundamental principles of the 1959 Revolution, and gradually recant them, which was the Adolfo Suarez Antillean however, observing its character, incompatible with political daring, and an orthodox trajectory in the Communist Party (PCC), apostasy is not foreseeable.
Born in 1960 in the province of Villa Clara, married twice and with two children from his first marriage, Miguel Mario Díaz-Canel moderated ideology in the communist youth. His ascension to the skies of the regime was rapid. The army general, Raúl Castro, incorporated him in 2003 to the political bureau of the PCC, where important decisions are made, cook, and continued his training as secretary of the party in the province of Holguín. Six years later, he is summoned to inform him of his appointment as Minister of Education.
In 2012, he becomes vice president of the Council of Ministers. A year later, the master's degree in revolutionary governance and the 21st century is completed with the position of vice president of the Council of State.He is not inexperienced in organic matters, he knows intrigues. He does not look like a brittle man because if he had feared that he was another, it would be his destiny.As Fidel Castro once explained, there were several leaders who let themselves be tempted by the siren songs of those who revived them to accelerate democracy in Cuba, those who today continue in relegation, living off revolutionary charity.
Carlos Aldana, Carlos Lage, Felipe Perez Roque, and other ministers and vice presidents, who were companions of the Castro brothers for many years, fell into misery when they were caught flirting with Western democracies, with the honeys of power. The punishment of Lage, 65, a former vice president of the State Council, was comparatively dignified: he resided in entrusting the campaign against the Aedes aegypti mosquito in a polyclinic in a Havana municipality.
Others did not have that luck.Diaz-Canel is made of another type of wood. He is not a foreigner, nor an improvised, nor a martyr for the freedom of Cuba. Certainly, his road map to the future is yet to be seen, but the route traveled in the circuits of the party and the institutions do not allow him to venture into his plans or betrayals of the cause, nor proposals for free.


Fundamentals of Animal Science is a research based text for college freshman, covers both companion and production animals and reviews key topics like reproduction, nutrition, genetics, and diseases at an introductory level.
Colin Guy Scanes is a Professor in the Department of Poultry Science at Mississippi State University where he has also served as Vice President for Research and Graduate Studies . He is also currently a faculty member of the Center of Excellence in Equine Science at Rutgers-The State University of New Jersey. Formerly, Dr. Scanes was a Professor in the Departments of Animal Science and Biomedical Science at Iowa State University. Among his awards and honors, Dr. Scanes received the Bouffault International Animal Agriculture Award in 2004, and is a Fellow of the American Association for the Advancement of Science and of the Poultry Science Association. Dr. Scanes is also the author or editor of 12 books, 68 reviews and book chapters, 263 refereed papers and 220 abstracts. In addition to his faculty duties, Dr. Scanes is the Editor-in-Chief of Poultry Science and a member of the editorial boards for Domestic Animal Endocrinology, The Journal of Experimental Zoology, Proceedings for the Society of Experimental Biology, Animal Biotechnology, General and Comparative Endocrinology, and Critical Reviews in Poultry Biology.


Join us At Dillard's in The Eastland Mall for our 3rd annual VIP Autumn Shopping experience Fall Into Fashion! Save the date for September 27th, 2015, from 6:30-9 p.m. Tickets are $20.00 and may be purchased here, at the Junior League of Evansville office, or at Dillard's.
The evening will once again include great fun with friends and door prizes; cash bar and hors d'oevres; style stages, make-up, and hair artist presentations–featuring the latest fashion trends! Exclusive store-wide discounts, swag bags and gifts with purchase will be offered as part of the evening. All proceeds are donated to the Junior League of Evansville.
On Tuesday, September 29th the Junior League of Evansville will present a live fashion giveback night benefiting the girls in the YWCA's Live Y'ers program. JLE members will provide a workshop on body image, self-esteem, and dressing for success in addition to mentors that will assist at-risk girls in selecting appropriate outfits. Each girl will receive new clothing thanks to the generosity of the Junior League of Evansville and the YWCA.


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Survey reveals best and worst cars of 2014
JD Power has announced the results of its 2014 What Car survey.
Ten of the UK's worst and best cars have been revealed as part of the 2014 What Car JD Power survey, which looked at ownership satisfaction from 15,000 motorists between January and March 2014.
On the Up. Get it?
The results, which take into account various facets of car ownership including fuel consumption, design, comfort, features, ownership costs, insurance, reliability and dealer satisfaction, reveal the UK's biggest winners and losers.
We'll start with the positives first. The Volkswagen Up! scored highest in the 2014 survey, with an impressive 85.1 per cent satisfaction score, narrowly beating the Volkswagen Golf Plus at 84.3 per cent and the jack-of-all-trades Skoda Yeti at 82.8 per cent.
Citroen's DS3 took home the hounour for best small car, while the large SUV category saw the Lexus RX come out on top. Best SUV went to the Skoda Yeti. Best family vehicle went to the Mercedes-Benz C-Class. The Kia Venga and Volvo V70/XC70 won in the small MPV and executive categories, respectively.
Skoda will be cracking out the champagne after getting two of its models into the top ten. The Skoda Octavia came in seventh place alongside the Lexus CT and Volvo V70/XC70 with a satisfaction rating of 81.2 per cent.
VW, meanwhile, managed four cars in the top ten, including the Scirocco and Tiguan on top of the aforementioned Up and Golf Plus. Perhaps unsurprisingly, VW is the parent company of Skoda. Jaguar snuck into tenth place with the XF, scoring 80.8 per cent.
The What Car JD Power survey also looked at over satisfaction by manufacturer. Jaguar and Lexus had the highest overall score at 80.8 per cent. Second-place Mercedes-Benz scored 80.1 per cent ahead of Skoda, which scored 80 per cent. VW closed out the top five with 79.1 per cent.
Now for the best bit. Owners of the Chevrolet Spark were by far the least satisfied with their purchase. Only the Alfa Romeo MiTo's score of 69.9 per cent comes close to the Spark's 67.6 per cent. Chevrolet managed to come last in the manufacturer standings, too, once again narrowly losing out to Alfa Romeo.
Other low-scorers include the Smart fortwo (102nd place), Fiat Grande Punto/Punto Evo (104th place), BMW X1 (71.4 per cent) and the Citroen C3 (71.4 per cent).
It's interesting to note the UK's most stolen car, the BMW X5, only satisfied owners to the tune of 75.2 per cent, landing it in 81st position behind the Audi Q3.
JD Power only surveyed models in the UK that had more than 4,000 sales and had been registered between January 2011 and December 2012. The Skoda Superb was 2013's winner (83 per cent) followed by the Skoda Yeti (82.8 per cent).


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Latest on DanceTabs
DanceTabs Contributors
A sharp eyed look at Dance & Ballet Across the World
Some News Items
Only Selective News Here – much more on Twitter
DanceTabs News
Sarasota Ballet – Digital Program 3: work by Darrell, Wright, Walsh, Wheeldon, Bourne & MacMillan
Stuttgart Ballet – The Sleeping Beauty – streamed from Stuttgart
Dance Memories of 2020 – London
English National Ballet – Echoes by Russell Maliphant, Michael Nunn and William Trevitt – streamed film
Author - Marina Harss
Marina Harss is a free-lance dance writer and translator in New York. Her dance writing has appeared in the New Yorker, The Nation, Playbill, The Faster Times, DanceView, The Forward, Pointe, and Ballet Review. Her translations, which include Irène Némirovsky's "The Mirador," Dino Buzzati's "Poem Strip," and Pasolini's "Stories from the City of God" have been published by FSG, Other Press, and New York Review Books. You can check her updates on Twitter at: @MarinaHarss
Tere O'Connor Dance – poem, Secret Mary – New York
Marina Harss
Tere O'Connor can be a frustrating choreographer. Look for structure and you'll likely be thwarted, frustrated, or worse. Because it clearly is there, but you can't understand it.
Kidd Pivot – The Tempest Replica – New York
Over the course of an hour and a half the story is told, more or less, twice: first in swift pantomime (aided by projections) and then in a series of more intensely danced interludes.
Interview: Herman Cornejo – American Ballet Theatre – Principal
Well, performing for me is really about that experience of giving to the audience. In the studio you work and perfect things, you collaborate with your partner, but for me it's about what happens on the stage, the ability to give something, to your partner, to the audience.
New York City Ballet – George Balanchine's The Nutcracker – New York
When something is beautifully made it never gets old. So it is with Balanchine's Nutcracker, first performed by New York City Ballet in 1954 and honed to near-perfection over the years.
Malavika Sarukkai – Stithi Gati, Raas, The Bird Song, Laya – New York
...once she begins to dance, words become irrelevant: the clarity and detail of her dancing leaves no room for ambiguity or doubt. Like a master story-teller she is able to change registers and points of view in the course of a single solo...
Trey McIntyre Project / Korea NCDC – Mixed bill – New York
The Unkindness of Ravens - collaborative efforts are not easy, especially when they are cooked up over a short period. The two companies are just similar enough - contemporary, ballet-based - to make the project even more complicated.
L.A. Dance Project – Moving Parts, Winterbranch, Quintett – New York
It's always been clear that Millepied is a man of intelligence and taste.
Works & Process at the Guggenheim – Royal Danish Ballet's new La Bayadere – New York
Hubbe - handsome and captivating as ever in tight black jeans and an Errol Flynn mustache - assured the Works and Process audience that the famously elegiac Shades scene has not been tampered with...
American Ballet Theatre – New Ratmansky, Symphony #9 – New York
Tonight's premiere of Ratmansky's newest work for American Ballet Theatre, Symphony #9, was cause for celebration. In fact, it left me feeling almost lightheaded, and terribly eager to see it again, as soon as possible.
American Ballet Theatre – Drink, Cruel World, Stars/Stripes, Rodeo, Pavane, Upper Room – New York
The highlight of the gala was the seventieth-anniversary performance of Agnes De Mille's Rodeo, preceded by a short film describing its creation, with archival footage of the hilariously histrionic, diminutive choreographer.
Interview: Shantala Shivalingappa – Dancer and Choreographer
Shivalingappa first caught my eye at the 2007 Fall For Dance festival, where she danced Varnam, a Kuchipudi solo. I was immediately struck by her musicality, the power and precision of her footwork, and the absolute clarity of her movements. And to the grace of her upper body and a jump that seems to comes out of nowhere, light and airy as a cat's.
Mark Morris Dance Group — Canonic ¾ Studies, A Wooden Tree, Silhouettes, Grand Duo — Sarasota
What was curious about A Wooden Tree is that it did not include much dancing in the traditional sense. It was as if Morris had decided to do an experiment: to make a dance with as little dancing as possible, practically a pantomime.
New York City Ballet – Two Hearts, Year of the Rabbit, Les Carillons – New York
There should be more nights like this at New York City Ballet.
Fall For Dance Festival – Program 4: Shivalingappa, PNWB, Melnick, Ka Leo O Laka – New York
Every Fall For Dance program is a bit of a pot luck, which is part of the festival's charm. This year it has been expanded to twelve of performances (each costing $15, up from $10 last year)...
New York City Ballet – Black and White program – New York
I've noticed two troubling trends this season at New York City Ballet. Perhaps they are connected. The first is the creeping tendency toward stolid tempi from the pit...
New York City Ballet – Stravinsky/Balanchine Firebird quad bill – New Work
The highlight of the program was the seldom-performed Divertimento from "Le Baiser de la Fée". It is a deceptively shadowy work, a fairy tale in the guise of a conventional divertissement.
Interview: Justin Peck – New York City Ballet – Dancer and Choreographer
"It's very lonely out there... I mean, it would be nice to have some sort of mentorship with regard to what it takes to be a choreographer."
Robert Wilson and Philip Glass – Einstein on the Beach – New York
This is the third mounting of the work since its première in 1976 at the Avignon Festival, where it quickly became the stuff of legend. What was this strange, endlessly-repetitive, oddly compelling work?
New York City Ballet – Apollo, Orpheus, Agon – New York
What is there to say about Orpheus, except that it seems to slip deeper into the recesses of time? I've read that at the première, the critic and poet Edwin Denby was so moved by it that he sat dumbfounded during intermission, unable to stand. It is difficult to imagine such a reaction today.
Mark Morris Dance Group – Dido and Aeneas – New York
It is something of a cliché to say it, but the guiding principle of Morris's Dido - as in the more recent Socrates - is simplicity. No single element - musc, words, dance - is privileged above the others.
Claudia Bauer | Deborah Weiss | Foteini Christofilopoulou | Graham Watts | Heather Desaulniers | Jann Parry | Josephine Leask | Lauren Gallagher | Lynette Halewood | Margaret Willis | Marina Harss | Oksana Khadarina | Sara Veale | Siobhan Murphy | Susanna Sloat | Valerie Lawson | Bruce Marriott (Ed)
The above list is composed of those whose work we feature regularly and have generally contributed in the last few months.
>> Complete list of DanceTabs Contributors and more info.
DanceTabs Tweets
Akram Khan (110) Alexei Ratmansky (221) American Ballet Theatre (280) Bach (105) Balanchine (121) Birmingham Royal Ballet (168) Bolshoi Ballet (181) Christopher Wheeldon (239) Coliseum (180) David H. Koch Theater (167) Don Quixote (151) English National Ballet (303) Frederick Ashton (273) George Balanchine (284) Giselle (232) Jerome Robbins (150) Kenneth MacMillan (258) Liam Scarlett (108) London (1705) Marianela Nunez (107) Mariinsky Ballet (149) Natalia Osipova (130) New York (545) New York City Ballet (325) Paris Opera Ballet (131) Petipa (143) Pina Bausch (122) Rambert (114) Romeo and Juliet (202) Royal Opera House (455) Rudolf Nureyev (109) Sadler's Wells (661) San Francisco (187) San Francisco Ballet (182) Scottish Ballet (120) Swan Lake (288) Tamara Rojo (128) Tchaikovsky (183) The Nutcracker (133) The Place (185) The Royal Ballet (636) The Sleeping Beauty (130) Vadim Muntagirov (108) Wayne McGregor (191) William Forsythe (133)
Jann Parry
Deborah Weiss
Lynette Halewood
Birmingham Royal Ballet – The Nutcracker at The REP – live streamed from Birmingham
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