10Pcs Nylon Hair Acrylic Watercolor Round Pointed Tip Artists Paint Brush Set. 100% brand new and high quality. Color: As the picture shows. Note: Due to the difference between different monitors, the picture may not reflect the actual color of the item. All you need to do is sending us the clear pictures to show it is broken. Please Note that size and color representation are subject to manufacturing variance and may not be exact. Also note that monitor settings may vary from computer to computer and may distort actual colors.
We believe that everything could be solved by communication. Feedback is simply what we pursue. We are in Shenzhen of Pearl River Delta which is developed place with economy and Trade We are a profession trade company. If you have any problems, we are very glad to help you. For the functional products in our shop (such as: Chargers, Data cables), which function is the same as description, but the actual color and shape may looks a little different from pictures because of the technical improvement and actual stock state.
The item "10Pcs Nylon Hair Acrylic Watercolor Round Pointed Tip Artists Paint Brush Set" is in sale since Wednesday, March 25, 2015. This item is in the category "Crafts\Art Supplies\Painting\Brushes, Palettes & Knives". The seller is "boss7321" and is located in Hong Kong.


JH Tee Antiques Ltd is pleased to offer a broad selection of Birks Laurentian pattern sterling flatware. You can purchase Birks Laurentian pattern sterling silver flatware online, or from our shop in Vancouver. To see more photos and details click on the image of one of the Laurentian pattern sterling pieces.


Greg Roman emailed over this recent video he did on Shepard Fairey... covering how skateboarding/ punk rock/ hip hop had on influencing him and his work.
Shepard talks about the influence graffiti, skateboarding and punk rock had on his life and the ways it shaped his art career. Key moments such as a school trip to NY and the melding of styles by brands such as Shut Skateboards allowed him to develop his techniques. Look out for some classic 90's skate footage as well.
STEN LEX, Caradente Museum, Spoleto.


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Home > Our Team > Sarika Dhanjal
Sarika Dhanjal
Head of Advice Quality
Sarika joined Progeny as the Head of Training & Competence in July 2021. Having previously worked at Tenet as the Head of Training & Mentoring, Sarika boasts a wealth of experience supporting new advisers. Her new role will see her drive clear, strategic learning and development pathways and support the next generation of professionals within the Progeny Academy, as well as hold responsibility for the delivery of Progeny's Training and Competence Scheme.
After studying computer science at the University of Leicester, Sarika graduated with a 2:1 and realised she wanted to follow a different career path. She applied for a job as a Personal Banking Adviser at Abbey National and moved to Leeds.
After 2 years at Abbey National, Sarika was ready for her next challenge and began her journey in regulated financial advice as a Financial Planning Manager at HSBC. She has since gone on to complete her QCF Level 4 diploma in regulated financial advice, along with her CeMap in order to provide holistic financial advice to her clients.
In 2014, Sarika joined Tenet and served in a variety of management roles over the years, including Training and Competence Supervisor, Paraplanning Manager, Operations Manager, and more recently her appointment as Head of Training and Mentoring. In was in this role where she had the opportunity to redesign and digitise induction programs for all new advisers joining the network and look at ways to help and support them through their learning journey.
In her spare time, Sarika loves to try new foods and eat out at new places. (Not quite as exotic as a bush tucker trial, but she does like something a bit different) She also enjoy days out with her kids and travelling. Most of her holidays have been based in Europe, but after venturing out further in 2019 to Mexico for her 10th wedding anniversary, she would love to visit a few more places further away…next on the list is Thailand.
LinkedIn: Sarika Dhanjal
News and advice from Sarika Dhanjal
Video | Redesigning the Progeny Adviser Academy

redpajama_set_name: RedPajamaCommonCrawl


Every day our organization strives to help people and companies, along with the world that we all share. SAM Recruitment recently created the We Care program to make a difference for vulnerable people, animals, and the environment.
Médecins Sans Frontières (Doctors without Borders/Artsen zonder Grenzen) is an international, medical humanitarian organisation, that provides medical assistance to people affected by conflict, epidemics, disasters, or exclusion from healthcare. They do so irrespective of race, religion, creed or political convictions. Their teams are made up of tens of thousands of health professionals, logistic and administrative staff - and their actions are guided by medical ethics and the principles of impartiality, independence and neutrality.


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Dzhalindite is a rare indium hydroxide mineral discovered in Siberia. Its chemical formula is In(OH)3.

It was first described in 1963 for an occurrence in the Dzhalinda tin deposit, Malyi Khingan Range, Khabarovskiy Kray, Far-Eastern Region, Russia.

It has also been reported from Mount Pleasant, New Brunswick, Canada; the Flambeau mine, Ladysmith, Rusk County, Wisconsin, US; in the Mangabeira tin deposit, Goiás, Brazil; Attica, mines of the Lavrion District, Greece; Erzgebirge, Saxony, Germany; the Krušné Hory Mountains of Bohemia, Czech Republic; the Chubu Region, Honshu Island, Japan; and the Arashan Massif of Tashkent, Uzbekistan.

References

Hydroxide minerals
Indium minerals
Cubic minerals
Minerals in space group 204
Minerals described in 1963

redpajama_set_name: RedPajamaWikipedia


At Fiberglass Insulation Local Experts, we'll be there to fulfill all of your goals regarding Fiberglass Insulation in Ellinwood, KS. Our workforce of skilled contractors can offer the service that you need with the most innovative solutions available. We make sure that you receive the best services, the most suitable price tag, and the finest supplies. Contact us today at 888-669-2230 to start.
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Placemaking During a Pandemic
Designer Takes
Technology may temporarily fill the void left by our now-shuttered offices, schools, and cultural institutions, but it will never fulfill our need for genuine human connection, says architect and urbanist Vishaan Chakrabarti
By Vishaan Chakrabarti
Illustration: Gabrielle Pilotti Langdon/Getty Images
As an architect who works each day to build human connectedness in cities, all for which I have ever fought is under prolonged attack by a diffuse enemy. Much of what we hold dear, from museums to chance sidewalk encounters, from Broadway shows to the subway, has become verboten. We have many metrics to measure economic loss, but we have not a single measure of cultural loss. I'm an architect today because of the cities and museums I visited as the child of academic parents with limited means; what children today are missing hard-to-afford visits to the Louvre or the Met? What art and artists are we losing to this virus? What cultural production is being sacrificed at this very moment?
For those of us lucky enough to be employed, we are now homebound, separated from our talented colleagues, knowing that our weeks if not months ahead will be filled with the intangible loss of face-to-face interactions. Yes, we should be thankful that modern telecommunications is advanced enough that I can have a video call in which my team of 24 can engulf my iPad in a grid of passport-sized vignettes reminiscent of The Brady Bunch, providing glimpses of brilliance that would otherwise in our studio be bursts of drawings, models, and fervent gesticulation. Last Friday we held a Zoom happy hour and had some good laughs making fun of each other's interiors. (All of us have learned much...perhaps too much...about everyone else's interior design choices.) My trainer can hound me via WhatsApp, and my daughter can have FaceTime sleepovers.
So should we be pleased that technology has risen to the occasion? Of course. Imagine the horror had this pandemic swept the globe 25 years ago. But do we for a moment think technology will ever take the place of human contact...that it will ever take the place of place? Of course not.
Ironically we are now testing the promises and pitfalls of technologies that have long sought to usurp the city. When the newly invented telephone became widespread, people claimed that cities would become unnecessary. Each subsequent telecommunications advent, from the fax machine to the internet, would claim the same, dangling the dream that cities would be replaced by people working from mountaintops, contented by nature, their families, and a lack of others. Yet these claims never came to pass; to the contrary, in recent years our cities have densified in the face of technological advancement to the point that today more people live in cities than any other form of human habitation. Cities didn't die; fax machines did.
Too many of us refuse to confront the truism that most humans need and want the company of other humans. History tells us that across the ancient world cities formed for reasons that were social, spiritual, and cultural, as much as economic, belying the idea that cities are some sort of necessary evil that will be made obsolete by technology.
Whatever the future holds, we cannot escape our desires to be physically proximate. Perhaps in response to this pandemic our futures may entail more staggered work hours split between office and home, or more remote learning when needed, or congested subways replaced by more walking and biking, but just as cities recovered from the Spanish Flu, Hurricane Katrina, and 9/11, we will recover from this pandemic still yearning for places of human connection; in fact it is already reminding us of how much we miss each other in the public commons. Because while there may be no place like home, there is also no home that is like a truly urban place.
Vishaan Chakrabarti, FAIA, is founder of the architecture and planning firm Practice for Architecture and Urbanism (PAU) and the incoming dean of College of Environmental Design at the University of California, Berkeley. He is the author of A Country of Cities: A Manifesto for an Urban America (2013) and has contributed to numerous media and cultural outlets, including TED and the New York Times.
What the World Needs From Designers in the 2020s
We asked 10 global thought leaders to remind us how design can make a difference
By Audrey Gray
Readers Respond: How Is Coronavirus Affecting Your Business?
We issued a call to see how the design community is faring during this uncertain time. Here is what you said
By Anna Fixsen
How Designers Are Keeping Their Businesses Running Amid an Outbreak
From Los Angeles to Hong Kong, we check in with 11 architects and designers to see how they are managing their staff and their businesses during this challenging time
By Stefanie Waldek
All the Design Events That Have Been Canceled or Postponed Due to Coronavirus So Far
Plus, the fairs planning to continue on as originally scheduled
By Alia Akkam and Madeleine Luckel


The Assessing office is located at City Hall, 300 W. Baraga Ave., Marquette. The assessing functions are to value all real and personal property within the corporate limits of the City as well as to annually prepare an assessment roll which lists all known property owners, legal descriptions, state equalized values and taxable values. If you have any questions of the assessing department, please stop in or call 906-225-8379 or 906-225-8381.
IMPORTANT, THE LAW HAS CHANGED REGARDING PERSONAL PROPERTY REPORTING & ASSESSMENT- PLEASE READ CAREFULLY.
Contrary to the understanding of many taxpayers, the new personal property tax exemptions do not provide for the complete exemption of business personal property. For 2019, all owners of personal property in Michigan must continue to file the appropriate forms with the Assessor where their property is located on December 31, 2018.
Those businesses with less than $80,000 True Cash Value of personal property owned, leased or used by them (or any related entity) within a tax-collecting municipality could qualify for an exemption if they timely filed Treasury Form 5076 "Affidavit of Owner of Eligible Personal Property Claiming Exemption". Exemption affidavits must be fully completed and filed with the local unit where the personal property is located no later than February 20, 2019 (postmark is acceptable).
All taxpayers must file in 2019 to claim the exemption. Once granted they will continue to receive the exemption until they no longer qualify for the exemption. Once they no longer qualify, the taxpayer is required to file a rescission form and a personal property statement no later than February 20th of the year that the property is no longer eligible. Failure to file the rescission form will result in significant penalty and interest as prescribed by P.A. 132 of 2018: "An owner who fails to file a rescission and whose property is later discovered to be ineligible for the exemption is subject to repayment of any additional taxes with interest at a rate of 1% per month or fraction of a month and penalties computed from the date the taxes were last payable without interest or penalty."
To determine whether or not a business qualifies for the exemption, it may be necessary to complete the "2019 Personal Property Statement" (Department of Treasury Form 632). If the total value (including related entities) after applying the published multipliers to owned, used and leased equipment original costs (True Cash Value) is less than $80,000 you may qualify for the exemption. However, you must claim the exemption by filing the affidavit by the February 20 deadline. Affidavits received after February 20, 2019 cannot be processed and will not receive the exemption, even if they would have otherwise been eligible.
Those businesses who have owned, leased, or borrowed personal property with a True Cash Value of $80,000 or more must complete the "2019 Personal Property Statement" and file by February 20, 2019. Assessments will be estimated by the Assessor if a Personal Property Statement is not filed or is filed late.
Additionally, in preparation for the start of the phase-out of eligible manufacturing personal property (beginning in 2017), manufacturers may be required to complete and file Treasury Form 5201 "Taxpayer Report of Property Claimed As Eligible Manufacturing Personal Property" beginning this year. Refer to the Personal Property Statement Instructions to determine if you are required to complete this additional form.
Forms are available in the Assessor's Office located at 300 W. Baraga Ave., Marquette, MI 49855. You may also contact us at (906) 225-8379 or (906) 225-8381.
Organizational meeting: Tuesday, March 5, 2019 9:00 a.m. to noon.
• Monday, March 11, 2019 9:00 a.m. to noon and 1:30 p.m. to 4:30 p.m.
• Tuesday, March 12, 2019 1:00 p.m. to 4:00 p.m. and 6:00 p.m. to 9:00 p.m.
City of Marquette guidelines and policy for granting or denying a poverty exemption is available at the City Assessor Office or on our website. If you feel you qualify for a poverty exemption on your property taxes, guidelines and applications are available on our website or at 300 West Baraga Avenue, Marquette, MI 49855. Applications must be filed with the Assessor BEFORE the MONDAY, MARCH 11, 2019 Board of Review Meeting. Any exemption granted will only apply to 2019 property taxes.
In order to be eligible for the exemption, the disabled veteran must have been honorably discharged from the armed forces of the United States. They must be a Michigan resident. Additionally, they must meet one of the following criteria: (a) Has been determined by the United States department of veterans' affairs to be permanently and totally disabled as a result of military service and entitled to veterans' benefits at the 100% rate. (b) Has a certificate from the United States veterans' administration, or its successors, certifying that he or she is receiving or has received pecuniary assistance due to disability for specially adapted housing. (c) Has been rated by the United States department of veterans' affairs as individually unemployable. The un-remarried surviving spouse of the disabled veteran is eligible for the exemption based upon the eligibility of their spouse; therefore the spouse must also be a Michigan resident. The exemption will continue only as long as the surviving spouse remains un-remarried. You must complete Form 5107 and attach all necessary proof of eligibility.
The City of Marquette will provide necessary, reasonable auxiliary aids and services, such as signers for the hearing impaired and audio tapes of printed materials being considered at the meeting, to individuals with disabilities at the meeting/hearing upon five (5) days notice to the City of Marquette. Individuals with disabilities requiring auxiliary aids or services should contact the City of Marquette by writing or calling the below name. Public notice will be given regarding any changes of the above meeting.


Abbas brings over 15 years in networking, cloud-based services, and multi-modal communications. He has helped create the vision for adopting intelligent communication strategies across business scenarios. He holds a BASc in Computer Engineering from the University of Toronto.
Machine learning will transform ChatOps—and IT Ops. Here's what to expect.
Tackling ChatOps at scale means refining the communication model. It requires buy-in from your team, but it's worth it when done right.


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BySultan
Humanitarian Relief,Yemen
Aid, Dates, KSrelief, Relief, Yemen
Climate Change,Foreign Affairs,Newsletter,UN
Now Online: November Edition Of Saudi Arabia In Focus Newsletter
KSA Mission Newsletter November 2016
This issue covers, amongst other things, the recent visit to Brussels of Dr Al-Rabeeah of the King Salman Humanitarian Aid and Relief Centre, our commitment to implementing the Paris Climate Agreement, the Saudi view on Iranian aggression and the controversial US JASTA bill, and our support of the UN's World Food Program.
Brussels, COP21, COP22, Iran, JASTA, KSrelief, Marrakech, Marrakesh, Riyadh, Saudi Arabia, UN, World Food Program
KSRelief's Supervisor General Al Rabeeah on Saudi Arabia's Humanitarian Aid & Relief Program
Dr Abdullah Al Rabeeah, Supervisor General of The King Salman Humanitarian Aid & Relief Centre (KSRelief) presented the details of the Kingdom's aid program in Brussels. Following meetings with EU Commissioner for Humanitarian Aid Christos Stylianides, Dr Al Rabeeah also took the time to explain the valuable work conducted by KSRelief as part of Saudi Arabia's role as one of the world's leading humanitarian donors.
The aims of the King Salman Humanitarian Aid and Relief Centre
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q1-Objective.mp4
KSRelief's main achievements since its foundation in 2015
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q2-Achievements.mp4
Key current areas of focus for KSRelief
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q3-Cooperation.mp4
The main challenges ahead for the Centre
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q4-Challenges.mp4
KSRelief's priorities for 2017
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q5-Priorities.mp4
A medical background at the heart of KSRelief's leadership
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q6-Personal-Background.mp4
Support for refugees in the Middle East
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q7-Refugees.mp4
Forging ties with the EU
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q8-Brussels.mp4
Dr Al Rabeeah's takeways from his trip to Brussels
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q9-Takeaways.mp4
How KSRelief and the EU's DG ECHO can learn from each other
http://ksamissioneu.wpengine.com/wp-content/uploads/2016/10/Q10-KSRelief-EU.mp4
Aid, Al Rabeeah, KSrelief, Refugees, Relief, Syria, Yemen
KSrelief: Our ongoing support for the women and children of Yemen
Since its inception, King Salman Humanitarian Aid and Relief Centre has done its best to assist those in need across all Yemeni governorates. The most important projects implemented by the centre and its partners have focussed on women and children. One of the centre's first projects involved providing medical care to more than 7,600 children and mothers living as refugees in Djibouti. This project was carried out in cooperation with UNICEF, with a total expenditure of €309,652.
Another project was put in place in response to an urgent UN flash appeal for immediate humanitarian assistance to Yemen; this €26.2 million project was again carried out in coordination with UNICEF. The project provided food, therapeutic and preventative interventions to save the lives of children under the age of five, and also provided protection for the health of pregnant and nursing mothers.
The table below contains statistics about programmes put in place by KSrelief in partnership with a number of United Nations organisations and national and international NGOs in support of women and children in the Republic of Yemen.
Aid category Children aided Women aided Total cost (US$)
Food security 7,033,441 4,974,873 129,740,269
Medical aid 6,774,269 5,187,790 49,669,086
Humanitarian aid 2,997,895 2,795,591 43,480,101
Humanitarian Relief, KSrelief, Yemen


Tiago Monteiro will hope to repeat his podium result from the previous round in Belgium this weekend at Monza, but admits that he is unsure how competitive he will be in the Italian round.
Unlikely to be racing with the new 1.6 litre turbo engine that is being prepared for use in SUNRED's SEAT Leon, Monteiro could find he is at a disadvantage against his rivals in terms of pace.
Despite this, Monteiro still hopes that he can get a decent result out of the weekend, both by getting the right setup on the car and also by being given a hand by the weather.
"Obviously we can try to take advantage of the slipstream in Monza's long straightaways, by lightening the aerodynamic package to minimize our handicap, but it won't be as easy as just that. The forecast for the weekend calls for rain and if that happens, it would certainly be a little nudge in the right direction to levelling the playing field.


Herzing University's Bachelor of Science in Business Management with a concentration in Marketing program is designed to center around sales, advertising, and communication within a series of elective, core, and specialized courses. Read more about this program now.
Herzing University's Bachelor of Science in Business Management with a concentration in Marketing program is designed to give students a look at what could be required of them if they wish to sell and advertise things in today's business world. This program is designed to help students learn how to interact with professionals and customers in a variety of business environments. By the end of their courses, students should be able to form business plans and know how to effectively execute them. In order to graduate from this program, students will be required to complete a capstone project or an internship as well as a series of personal and professional development courses. This program is available online and at select campuses.
Individuals interested in applying to this program should have excellent interpersonal skills that they wish to apply to business situations. Students should also be able to express themselves effectively and convincingly, both in written and verbal mediums. Though requirements may vary, applicants should have earned a high school diploma or equivalent.
This Herzing University program consists of at least 123 credit hours. These hours are distributed between management core courses, marketing courses, electives, an internship / capstone project, and personal professional development courses.
A minimum of 12.00 semester credit hours of open electives is required. At least 3.00 semester credit hours must be 300- to 400-level courses.
Graduates of this Herzing University program could be able to seek entry-level business jobs or jobs that might offer greater challenges. By using their interpersonal, organizational, and persuasive skills, graduates could pursue work with large companies or smaller organizations. They may even be able to go into work for themselves as a consultant or an entrepreneur.
Upon graduating from this program, students could find that it is beneficial to their long-term goals to pursue a higher-level degree. By applying to a higher-level degree program, they could be able to broaden their business knowledge while honing their existing marketing abilities. If a student wants to seek opportunities as an executive manager or as the head of a marketing department, a master's degree or higher may be required.


What can we make out of a global entity claiming to be the custodians of peace, justice and equality towards all member states, refusing to accept evidence, denies to investigate requests & appeals & completely ignores appeals by citizens of member states? Well, if the global entity is the UN and the aggrieved member states are from the Third World, then it's a one-way street because the UN is steered by white-supremacists & their local worshippers and there's nothing the black, brown or yellow people can do, unless they unite & refuse to be re-colonized & return to the days where white rule of bloodshed, plunder & persecutions prevailed.
Black nations are being hounded by the ICC and nations of Asia by the UNHRC. Bloc nations in the UN in the form of the EU & OIC get away with the might of their money, political & trade advantage, population & religious outreach leaving all others prey to the UN system & international NGO network.
Countries that make profit from arms manufacture also makes sure there are plenty of conflicts to sell arms generally to both parties. Peace doves in the form of UN peace keepers, NGO organizations replace the traditional intelligence operatives who are sent to register under various missions/visions with the goal of collecting data & statistics to be used for various future strategic/tactical operations against any state that does not conform to the wish of the powerful nations. Assassinations of leaders, using subtle methods to buy over politicians (money, sexual weakness etc), state officials, influencing & roping in academics, media and any other locals who may pose a threat to their overall plans are orchestrated with precision and plenty of funding despatched for the purpose.
The arm twisting & diplomatic bullying is just a carry forward of how the white nations ruled over the world for over 500 years under colonial rule. Let's not forget that every law that everyone has to currently abide by is what these white nations approved & sanctioned to use. All the indigenous laws, cultures & systems were completely ignored when international laws & regulations were drafted. It is only the OIC majority Muslim states that continue to resist international laws & continue to apply archaic laws associated with their faith. All other nations have had no choice but to nod agreement to and will not dare question why their traditional & indigenous laws, systems & cultures are being ignored & they are forced to follow something foreign which the natives opposed and sacrificed their lives during colonial rule. The West has been quick to formulate their own defence through local Kalu-Suddas or sepoys willing worshippers of anything imported from West.
Having taken stock of the reactions of the natives and studied the mentality of the natives the West has over the years implemented enough of programs especially on brainwashing & influencing the enemy. Therefore, they have enough of ways & means to subjugate the enemy.
What is currently taking place using the UN & its puppets in office is the virtual return of white supremacy in virtually all domains of national governance. The calls for hybrid court is nothing but a message that 'you locals are not good enough, and we west have the right to rule over you'. The rejection of local judges in place of foreign judges connotes the same message. If the judiciary, law, penal codes are all being changed and nations are losing the Westphalian sovereign rights why not have hybrid legislation and dethrone the nincompoops in Parliament too…. We have had enough of their treacherous and corrupt rule anyways. Not that there is no corruption in the West in government or private sectors, in fact the West are the one's that taught the rest of the world how to loot.
However, Sri Lankan citizens are aghast & many who are listening to Admiral Sarath Weerasekera & the Global Sri Lankan Forum Team battle out inside the halls of the UNHRC with legally sound arguments & evidence against the UNHRC Resolutions, must all be wondering why the UN & the world are not listening to the other side & accepting only the version of terrorists. Of course, the LTTE global kitty has bought a lot of influence, but over and above that we all need to understand that Sri Lanka is vulnerable at political & trade levels simply because of its geographical positioning. Countries eye Sri Lanka because it sees potential that our leaders do not see, refuse to see or they see but prefer to barter it for their personal gains instead of nurture it for the citizens and posterity.
At another more important & ignored level to understand why these Western bloc nations are going on a witch hunt against Sri Lanka with fabrications, cooked up stories & well-funded reports is that these countries continue to be plagued with the thought that 'we are better' and 'our job is to civilize you', remember the 'white man's burden' version by Kipling. That very much prevails still. Let's also not forget that these white nations were very angry that their calls to stop the military onslaught and refrain from defeating the LTTE was not adhered to. Revenge has been very much in line with all of the successive resolutions and threats accompanying these UN orders.
Joseph Parrott writing to the Washington Post How white supremacy went global" has explained the scenario & excerpts will provide clues for us to understand the nature of the global decisions being made through the UN & diplomatically.
Tragically, national leaders representing the black, brown & yellow man have failed to stand up against the white supremacy. They have not understood the numerical dynamics of the Third World out numbering a handful of white nations bullying the rest of the world using money, arms & political influence through systems they have created & representatives they have appointed on their behalf. Standing up against the white supremacy is not to replace white with non-white rule but to clearly establish that justice means justice, equality should mean equality and it should not be in the format that the UN system currently dishes out to the rest of the world playing pied piper to the EU & OIC bloc of nations.
Thankfully Japan has said there is no requirement for foreign judges, can the rest of the Third World look at the evidence presented by the Global Sri Lanka Forum instead of relying on lies & distortions by LTTE terrorists & those that survive on LTTE money!
Well, it is unfortunate for the rest of the world( all the other countries but the West), that the leaders, with the exception of Russia, China and maybe some South American countries including Cuba, look like spineless in the face of threats from the West that they bend too much backward in fear of retribution if they don't follow the West's agenda. Just the countries that comprise the BRICKS along with the support of nations like Iran and Syria that can take care of the oil needs of those involved are enough to counter any military, economic and political threats posed by those Western powers! Who would take the lead for that? I believe the purpose for the said BRICKS is just the same mentioned above, but it doesn't look like moving ahead!
What I see is High Caste Indian Supremacy.
There are plenty of Indian Colonial Parasites acting against us in the open and behind the scene.
Navanatham Pillayan is not a white and the same applies to Yasmin Sooka.
There are plenty of Indian Parasites Like Athul Keshap the US Ambassador.
Ms Waduge, my apologies for making a comment outside the subject matter of your excellent article. But I believe the matter is of criticial importance to all Sri Lankans and I really had not idea of where else on Asianweb I could post my comment.
The questions that screams to be answered is whether Cambridge Analytics played a role in the regime-change operation of 08th January 2015 in our Motherland.
Error : It should read 'LankaWeb' and not 'Asianweb'. Apologies to all.
There are Indian Parasites in UK, USA, Canada and Europe who are very influential.
Sinhalese should see what Indians are doing to us.
I know this because I have come across Indians from India as well as Indians from Indian Colonies like ours, Mauritius, Malaysia, South Africa.
It is the Indians who sucked us off and gave some to the whites they kept most of it.
Nagasaki, 1.5 million in Vietnam alonoe, Iraq etc. etc millions; then uk during the colonial era millions of natives, partition of india alone cost more than million lives. And they still doing it to poor countries cladestinely). So bogus prince & co. busy harassing Sri Lanka while that bogus prince's tail is on fire caught up in the mass murders in syria and yemen. Millions of people have died/displaced over 7 years, and still bogus prince & co can't see it. Don't want to see it since syria – Russia involved. So it is a no no of course.
see these mass murders happening in syria, yemen etc.
Lanka gets the full bullying by the bogus human rights monkeys.
we ever had. That's why they are called Sinhala modayas!
human rights, animal rights etc. An honest, principled person! Not these bogus prince and its bogus police who can't see its tail on fire (syria, yemen, libya, iraq etc. etc. burning)! Ashamed? Not a bit of course.
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redpajama_set_name: RedPajamaGithub


Our continuing strong support for a Medicare-for-all, single-payer health care system derives from the fact that as long as the U.S. health insurance industry is driven by profit, it will be impossible to provide health care as a right for everyone.
For-profit health insurance industries are largely, if not completely, absent in all countries that have universal health care.
It is less than surprising to us that, shortly after the passage of President Barack Obama's health insurance legislation, a coalition that had strongly supported the bill - Health Care for America Now (HCAN), representing 1,000 organizations - issued a report strongly criticizing the U.S. health insurance industry for the over-the-top, increased compensation of its CEOs in 2009.
In a report published on Aug. 11, 2010, HCAN had many negative things to say about the industry, which was empowered and enriched by the legislation the organization and Obama had supported.
In 2009, while America's families struggled with skyrocketing health insurance costs and the worst economy since the Great Depression, chief executives of the 10 largest for-profit health insurance companies collected total pay of $228.1 million, up from $85.5 million the year before.
Compensation for health-insurance CEOs in 2009 was enough to pay for every resident of Philadelphia, Dallas and Minneapolis combined (3.2 million people) to go to their regular doctor for an office visit.
CIGNA CEO Edward Hanway, who retired in December 2009, got his usual excessive pay and stock options that year, but also gave himself a $111 million pension package as a going-away gift. Combined with pay received by his successor, David Cordani, the company parted with $136 million in CEO compensation in 2009. That is enough money to pay for 204,000 infants to receive the recommended series of seven well-baby visits in their first year.
The insurance companies are driven by perverse incentives that reward CEOs for imposing devastating rate hikes on families and businesses while denying care to people when they need it most.
It is our view that the public in this country, however, seems to "get it" even more than organizations such HCAN, members of Congress or the president.
An annual Harris poll asks, "Which of these industries do you think are generally honest and trustworthy - so that you normally believe a statement by a company in that industry?" In the most recent survey, in the fall of 2009, only 7 percent of the 2,300 people randomly selected by the poll answered "yes" for trusting the health insurance industry. In other words, only 1 out of 14 people believes the industry to be honest and trustworthy.
What more is there to say? It is time to rid this country of its main obstacle in providing health care for everyone: the health insurance industry.
For more information about the work of Public Citizen and other organizations on this subject, visit www.citizen.org/singlepayer.


Jenn and Romy: Finally, We Have a Memory Foam Mattress!
Hi, my girlfriend and I are thinking about buying the same mattress (Nature's Sleep St Bart 8"), but are a little skeptical, because reviews are hard to find anywhere other than on the Nature's Sleep website. How has your experience been? Is it pretty firm? Has it started to sag where you sleep? I have some back problems and our old mattress is killing me, but we want to make an educated decision. Thanks for your help!
We've had the mattress for a while now and I absolutely love it! No problems at all. Its comfy and holding up just fine. No sagging. When I first decided to buy it, I was skeptical because it was one of the cheaper options, but I am happy I bought it.
Would you call it a firm mattress? Did it have a break in period, after which you considered it softer?
I would say its on the firm side, however, the actual level of firmness varies depending on the temperature. This is true of all foam mattresses. On cold winter nights, the mattress is a bit firmer when you first lay down until your body heat warms it up and then it gets a little softer, but in general I would consider it on the firm side. In summer, when its warm, the mattress is a bit softer. At the same time, its kind of a hybrid because you sink into it a pressure points, which I like. I didn't find any break-in period. Its pretty much the same and I haven't noticed any change in the mattress getting softer over time.
Memory Foam Mattresses are the top most mattress which are used by the people in all over the world, because that type of mattress are used to get a smooth and luxury sleep at night. There are many types of mattresses are available for sale but they are not much more comfortable then a very perfect Memory foam mattress topper which are truly made for just giving you the comfortable, enjoyable and peaceful sleep at the night, and this night sleep fresh your all day after waking up in the morning.
According to the Thao Experts or Thao Mattress Experts after the long working hour of the day, your body just needs a comfortable sleep for getting ready for next day, and the common or cheap mattress of your bad is not able to provide you comfortable sleeping at night which your body needs. So always choose the best sleeping bed mattress for you which give you comfortable sleep at night.
I always feel comfortable to sleep on the newly bought mattress, and I won't deny that I steal the idea from www.FurnitureUK.co.uk as it described all about the best memory foam mattress.
Great post. Visit mattress store silver spring to have great collection of mattress.
Oh very nice mattress, Looking great on you bed. You can buy Custom Furniture Miami to make you house more beautiful and stylish.
A mattress is something which makes you comfortable while sleeping so if you are looking for mattress then visit mattress fabric where you can find good quality of the mattress.
That's wonderful! Mattress-in-a-box companies ignore the fact that both sleepers may desire a different firmness for their side of the bed. Sleep on a custom foam mattress from Morphiis for the best sleep you've ever had.


Jhpiego believes that successful, sustainable health care services can only be achieved through solid partnerships. We work with a broad spectrum of international, national and community-level groups. Jhpiego attributes much of its success to maintaining key relationships with governments, donors, business partners, subcontractors, professional associations, peers and colleagues. At Jhpiego, these relationships are highly valued.
Jhpiego collaborates with local ministries of health, nongovernmental organizations, faith-based organizations, hospitals and clinics, and communities in the countries where we work. Jhpiego often needs both public- and private-sector partners for its proposals to donors. We rely on a variety of funders worldwide. We actively seek partners who wish to work with Jhpiego on specific country/regional or health area needs for our overseas work.


text:
Neutropenia
(Redirected from Neutropaenia)
Blood film with a striking absence of neutrophils, leaving only red blood cells and platelets
Infectious disease, Hematology
Aplastic anemia, Glycogen storage disease, Cohen syndrome,[1][2] gene mutations
Diagnostic method
CBC[3]
Antibiotics, Splenectomy if needed,[3] G-CSF
Neutropenia is an abnormally low concentration of neutrophils (a type of white blood cell) in the blood.[4] Neutrophils make up the majority of circulating white blood cells and serve as the primary defense against infections by destroying bacteria, bacterial fragments and immunoglobulin-bound viruses in the blood.[5] People with neutropenia are more susceptible to bacterial infections and, without prompt medical attention, the condition may become life-threatening (neutropenic sepsis).[6]
Neutropenia can be divided into congenital and acquired, with severe congenital neutropenia (SCN) and cyclic neutropenia (CyN) being autosomal dominant and mostly caused by heterzygous mutations in the ELANE gene (neutrophil elastase).[7] Neutropenia can be acute (temporary) or chronic (long lasting). The term is sometimes used interchangeably with "leukopenia" ("deficit in the number of white blood cells").[8]
1 Signs and symptoms
2 Causes
3 Pathophysiology
4 Diagnosis
5 Treatment
6 Prognosis
Signs and symptoms[edit]
Signs and symptoms of neutropenia include fever, painful swallowing, gingival pain, skin abscesses, and otitis. These symptoms may exist because individuals with neutropenia often have infection.[3]
Children may show signs of irritability and poor feeding.[9] Additionally, hypotension has also been observed in individuals who suffer from this condition.[6]
Causes[edit]
The causes of neutropenia can be divided between problems that are transient and those that are chronic. Causes can be divided into these groups:[1][2][10][11]
Chronic neutropenia:
Evans syndrome
Felty syndrome
HIV/AIDS infection
Glycogen storage disease
Cohen syndrome
Congenital immunological disorder, e.g. ELA2 mutation, GATA2 deficiency
Barth syndrome
Pearson syndrome
Pudlak syndrome
Transient neutropenia:
Penicillamine
Trimethoprim/sulfamethoxazole
Valproate
Gram-positive bacteria are present in 60–70% of bacterial infections. There are serious concerns regarding antibiotic-resistant organisms. These would include as methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus (VRE).[12])
Other causes of congenital neutropenia are Shwachman–Diamond syndrome, Cyclic neutropenia, bone marrow failure syndromes, cartilage–hair hypoplasia, reticular dysgenesis, and Barth syndrome. Viruses that infect neutrophil progenitors can also be the cause of neutropenia. Viruses identified that have an effect on neutrophils are rubella and cytomegalovirus.[1] Though the body can manufacture a normal level of neutrophils, in some cases the destruction of excessive numbers of neutrophils can lead to neutropenia. These are:[1][citation needed]
Bacterial or fungal sepsis
Necrotizing enterocolitis, circulating neutrophil population depleted due to migration into the intestines and peritoneum
Alloimmune neonatal neutropenia, the mother produces antibodies against fetal neutrophils
Inherited autoimmune neutropenia, the mother has autoimmune neutropenia
Autoimmune neutropenia of infancy, the sensitization to self-antigens
Pathophysiology[edit]
The pathophysiology of neutropenia can be divided into congenital and acquired. The congenital neutropenia (severe and cyclic type) is autosomal dominant, with mutations in the ELA2 gene (neutrophil elastase) as the most common genetic reason for this condition.[7] Acquired neutropenia (immune-associated neutropenia) is due to anti-neutrophil antibodies that target neutrophil-specific antigens, ultimately altering neutrophil function.[13] Furthermore, emerging research suggests neutropenia without an identifiable etiology (idiopathic neutropenia) may be the result of a low-grade, chronic inflammatory process with an abnormal excessive production of myelosuppressive cytokines in a study conducted in the island of Crete.[14]
Neutropenia fever can complicate the treatment of cancers. Observations of children noted that fungal infections are more likely to develop in those with neutropenia. Mortality increases during cancer treatments if neutropenia is also present.[6] Congenital neutropenia is determined by blood neutrophil counts (absolute neutrophil counts or ANC) < 0.5 × 109/L and recurrent bacterial infections beginning very early in childhood.[15]
Congenital neutropenia is related to alloimmunization, sepsis, maternal hypertension, twin-to-twin transfusion syndrome, and Rh hemolytic disease.[1]
Diagnosis[edit]
P anca
Neutropenia can be the result of a variety of consequences, including from certain types of drugs, environmental toxins, vitamin deficiencies, metabolic abnormalities, as well as cancer or infections. Neutropenia itself is a rare entity, but can be clinically common in oncology and immunocompromised individuals as a result of chemotherapy (drug-induced neutropenia). Additionally, acute neutropenia can be commonly seen from people recovering from a viral infection or in a post-viral state. Meanwhile, several subtypes of neutropenia exist which are rarer and chronic, including acquired (idiopathic) neutropenia, cyclic neutropenia, autoimmune neutropenia, and congenital neutropenia.[medical citation needed]
Neutropenia that is developed in response to chemotherapy typically becomes evident in seven to fourteen days after treatment. Conditions that indicate the presence of neutropenic fever are implanted devices; leukemia induction; the compromise of mucosal, mucociliary and cutaneous barriers; a rapid decline in absolute neutrophil count, duration of neutropenia >7–10 days, and other illnesses that exist in the patient.[12]
Signs of infection can be subtle. Fevers are a common and early observation. Sometimes overlooked is the presence of hypothermia, which can be present in sepsis. Physical examination and accessing the history and physical examination is focussed on sites of infection. Indwelling line sites, areas of skin breakdown, sinuses, nasopharynx, bronchi and lungs, alimentary tract, and skin are assessed.[12]
The diagnosis of neutropenia is done via the low neutrophil count detection on a complete blood count. Generally, other investigations are required to arrive at the right diagnosis. When the diagnosis is uncertain, or serious causes are suspected, bone marrow biopsy may be necessary. Other investigations commonly performed: serial neutrophil counts for suspected cyclic neutropenia, tests for antineutrophil antibodies, autoantibody screen (and investigations for systemic lupus erythematosus), vitamin B12 and folate assays.[16][17] Rectal examinations are usually not performed due to the increased risk of introducing bacteria into the blood stream and the possible development of rectal abscesses. A routine chest X-ray and urinalysis may be can not be relied upon or considered normal due to the absence of neutrophils.[12]
Classification[edit]
Neutrophil
Generally accepted reference range for absolute neutrophil count (ANC) in adults is 1500 to 8000 cells per microliter (µl) of blood. Three general guidelines are used to classify the severity of neutropenia based on the ANC (expressed below in cells/µl):[18]
Mild neutropenia (1000 <= ANC < 1500): minimal risk of infection
Moderate neutropenia (500 <= ANC < 1000): moderate risk of infection
Severe neutropenia (ANC < 500): severe risk of infection.
Each of these are either derived from laboratory tests or via the formula below:
ANC = ( % n e u t r o p h i l s + % b a n d s ) × ( W B C ) ( 100 ) {\displaystyle (\%neutrophils+\%bands)\times (WBC) \over (100)} [1][18][19]
Treatment[edit]
Recombinant granulocyte-colony stimulating factor preparations, such as filgrastim[20] can be effective in people with congenital forms of neutropenia including severe congenital neutropenia and cyclic neutropenia;[21] the amount needed (dosage) to stabilize the neutrophil count varies considerably (depending on the individual's condition).[22] Guidelines for neutropenia regarding diet are currently being studied.[23]
Most cases of neonatal neutropenia are temporary. Antibiotic prophylaxis is not recommended because of the possibility of encouraging the development of multidrug-resistant bacterial strains.[1]
Neutropenia can be treated with hematopoietic growth factors, granulocyte-colony stimulating factor (G-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF). These are cytokines (inflammation-inducing chemicals) that are present naturally in the body. These factors are used regularly in cancer treatment with adults and children. The factors promote neutrophil recovery following anticancer therapy.[1]
The administration of intravenous immunoglobulins (IVIGs) has had some success in treating neutropenias of alloimmune and autoimmune origins with a response rate of about 50%. Blood transfusions have not been effective.[1]
Prognosis[edit]
If left untreated, people with fever and absolute neutrophil count <500 have a mortality of up to 70% within 24 hours.[12] The prognosis of neutropenia depends on the cause. Antibiotic agents have improved the prognosis for individuals with severe neutropenia. Neutropenic fever in individuals treated for cancer has a mortality of 4-30%.[24]
Epidemiology[edit]
Neutropenia is usually detected shortly after birth, affecting 6% to 8% of all newborns in neonatal intensive care units (NICUs). Out of the approximately 600,000 neonates annually treated in NICUs in the United States, 48,000 may be diagnosed as neutropenic. The incidence of neutropenia is greater in premature infants. Six to fifty-eight percent of preterm neonates are diagnosed with this auto-immune disease. The incidence of neutropenia correlates with decreasing birth weight. The disorder is seen up to 38% in infants that weigh less than 1000g, 13% in infants weighing less than 2500g, and 3% of term infants weighing more than 2500 g. Neutropenia is often temporary, affecting most newborns in only first few days after birth. In others, it becomes more severe and chronic indicating a deficiency in innate immunity.[1]
Furthermore, the prevalence of chronic neutropenia in the general public is rare. In a study conducted in Denmark, over 370,000 people were assessed for the presence of neutropenia. Results published demonstrated only 1% of those evaluated were neutropenic, and were commonly seen in those suffering from HIV, viral infections, acute leukemias, and myelodsyplastic syndromes. The study concluded the presence of neutropenia is an ominous sign that warrants further investigation and follow-up.[25]
Pancytopenia
^ a b c d e f g h i j Ohls, Robin (2012). Hematology, immunology and infectious disease neonatology questions and controversies. Philadelphia, PA: Elsevier/Saunders. ISBN 978-1-4377-2662-6: Access provided by the University of Pittsburgh
^ a b Newburger, Peter E.; Dale, David C. (1 July 2013). "Evaluation and Management of Patients with Isolated Neutropenia". Seminars in Hematology. 50 (3): 198–206. doi:10.1053/j.seminhematol.2013.06.010. ISSN 0037-1963. PMC 3748385. PMID 23953336.
^ a b c "Neutropenia Clinical Presentation: History, Physical Examination". emedicine.medscape.com. Retrieved 8 December 2015.
^ "Neutropenia". National Center for Biotechnology, National Library of Medicine. Retrieved 8 December 2015.
^ "Neutrophils". National Center for Biotechnology, National Library of Medicine. Retrieved 8 December 2015.
^ a b c Fredricks, David N; Fung, Monica; Kim, Jane; Marty, Francisco M.; Schwarzinger, Michaël; Koo, Sophia (2015). "Meta-Analysis and Cost Comparison of Empirical versus Pre-Emptive Antifungal Strategies in Hematologic Malignancy Patients with High-Risk Febrile Neutropenia". PLOS ONE. 10 (11): e0140930. Bibcode:2015PLoSO..1040930F. doi:10.1371/journal.pone.0140930. ISSN 1932-6203. PMC 4640557. PMID 26554923.
^ a b Marshall S. Horwitz; et al. (2013). "ELANE Mutations in Cyclic and Severe Congenital Neutropenia". Hematology/Oncology Clinics of North America. 27 (1): 19–41. doi:10.1016/j.hoc.2012.10.004. PMC 3559001. PMID 23351986. Retrieved 28 October 2018.
^ Boxer, Laurence A. (8 December 2012). "How to approach neutropenia". ASH Education Program Book. 2012 (1): 174–182. doi:10.1182/asheducation.v2012.1.174.3798251. ISSN 1520-4391. PMID 23233578.
^ Hazinski, Mary Fran (4 May 2012). Nursing Care of the Critically Ill Child. Elsevier Health Sciences. p. 835. ISBN 978-0323086035.
^ Donadieu J, Beaupain B, Fenneteau O, Bellanné-Chantelot C (November 2017). "Congenital neutropenia in the era of genomics: classification, diagnosis, and natural history". British Journal of Haematology. 179 (4): 557–574. doi:10.1111/bjh.14887. PMID 28875503.
^ Muturi-Kioi, Vincent; Lewis, David; Launay, Odile; Leroux-Roels, Geert; Anemona, Alessandra; Loulergue, Pierre; Bodinham, Caroline L.; Aerssens, Annelies; Groth, Nicola; Saul, Allan; Podda, Audino (4 August 2016). "Neutropenia as an Adverse Event following Vaccination: Results from Randomized Clinical Trials in Healthy Adults and Systematic Review". PLoS ONE. 11 (8): e0157385. Bibcode:2016PLoSO..1157385M. doi:10.1371/journal.pone.0157385. ISSN 1932-6203. PMC 4974007. PMID 27490698.
^ a b c d e Williams, Mark (2007). Comprehensive hospital medicine an evidence based approach. Philadelphia: Saunders Elsevier. ISBN 978-1-4160-0223-9; Access provided by the University of Pittsburgh
^ Schwartzberg, Lee S. (1 January 2006). "Neutropenia: etiology and pathogenesis". Clinical Cornerstone. 8 Suppl 5: S5–11. doi:10.1016/s1098-3597(06)80053-0. ISSN 1098-3597. PMID 17379162. – via ScienceDirect (Subscription may be required or content may be available in libraries.)
^ Dale, David C.; Bolyard, Audrey Anna (2017). "An update on the diagnosis and treatment of chronic idiopathic neutropenia". Current Opinion in Hematology. 24 (1): 46–53. doi:10.1097/MOH.0000000000000305. PMC 5380401. PMID 27841775.
^ Makaryan, Vahagn; Rosenthal, Elisabeth A.; Bolyard, Audrey Anna; Kelley, Merideth L.; Below, Jennifer E.; Bamshad, Michael J.; Bofferding, Kathryn M.; Smith, Joshua D.; Buckingham, Kati; Boxer, Laurence A.; Skokowa, Julia; Welte, Karl; Nickerson, Deborah A.; Jarvik, Gail P.; Dale, David C. (2014). "TCIRG1-Associated Congenital Neutropenia". Human Mutation. 35 (7): 824–827. doi:10.1002/humu.22563. ISSN 1059-7794. PMC 4055522. PMID 24753205.
^ Levene, Malcolm I.; Lewis, S. M.; Bain, Barbara J.; Imelda Bates (2001). Dacie & Lewis Practical Haematology. London: W B Saunders. p. 586. ISBN 978-0-443-06377-0.
^ "Neutropenic Patients and Neutropenic Regimes | Patient". Patient. Retrieved 8 December 2015.
^ a b Hsieh MM, Everhart JE, Byrd-Holt DD, Tisdale JF, Rodgers GP (April 2007). "Prevalence of neutropenia in the U.S. population: age, sex, smoking status, and ethnic differences". Ann. Intern. Med. 146 (7): 486–92. doi:10.7326/0003-4819-146-7-200704030-00004. ISSN 0003-4819. PMID 17404350.
^ "Absolute Neutrophil Count Calculator". reference.medscape.com. Retrieved 8 December 2015.
^ Schouten, H. C. (3 October 2006). "Neutropenia management" (PDF). Annals of Oncology. 17 (suppl 10): x85–x89. doi:10.1093/annonc/mdl243. PMID 17018758. Retrieved 8 December 2015.
^ James, R M; Kinsey, S E (1 October 2006). "The investigation and management of chronic neutropenia in children". Archives of Disease in Childhood. 91 (10): 852–858. doi:10.1136/adc.2006.094706. ISSN 0003-9888. PMC 2066017. PMID 16990357.
^ Agranulocytosis—Advances in Research and Treatment: 2012 Edition: ScholarlyBrief. ScholarlyEditions. 26 December 2012. p. 95. ISBN 9781481602754.
^ Jubelirer, S. J. (6 April 2011). "The Benefit of the Neutropenic Diet: Fact or Fiction?". The Oncologist. 16 (5): 704–707. doi:10.1634/theoncologist.2011-0001. PMC 3228185. PMID 21471277.
^ "Neutropenia". eMedicine. MedScape. Retrieved 8 December 2015.
^ Andersen, C. L.; Tesfa, D.; Siersma, V. D.; Sandholdt, H.; Hasselbalch, H.; Bjerrum, O. W.; Felding, P.; Lind, B.; Olivarius, Niels de F.; Palmblad, J. (2016). "Prevalence and clinical significance of neutropenia discovered in routine complete blood cell counts: a longitudinal study". Journal of Internal Medicine. 279 (6): 566–575. doi:10.1111/joim.12467. PMID 26791682.
ICD-10: D70
ICD-9-CM: 288.0
DiseasesDB: 8994
MedlinePlus: 007230
eMedicine: med/1640
Main cell types in blood
Myeloid
CFU-G
Band cell
Basophil
CFU-Baso
Eosinophil
CFU-Eos
Mast cell
CFU-Mast
Monocytes
Macrophages
Histiocytes
Kupffer cells
Alveolar macrophage
Epithelioid cells
giant cells
Langhans giant cells, Foreign-body giant cell
Touton giant cells
Antigen presenting cells
Langerhans cell
CFU-DL
CFU-M
CFU-Meg
Megakaryoblast
Promegakaryocyte
Megakaryocyte
Reticulocyte
Normoblast
CFU-E
Precursor cells
CFU-GM
Megakaryocyte–erythroid progenitor cell
CFU-GEMM
Myelomonocyte
Hematopoietic system
Hematopoietic stem cell
Hematologic disease: Monocyte and granulocyte disease (CFU-GM/CFU-Baso/CFU-Eos), including immunodeficiency (D70-D71, 288)
Monocytes/
Histiocytosis
-cytosis:
Monocytosis
-penia:
Monocytopenia
granulocytosis
Neutrophilia
Eosinophilia/Hypereosinophilic syndrome
Basophilia
Bandemia
Granulocytopenia/agranulocytosis (Neutropenia/Severe congenital neutropenia/Cyclic neutropenia
Eosinopenia
Basopenia)
chemotaxis/degranulation:
Leukocyte adhesion deficiency
Chédiak–Higashi syndrome
Neutrophil-specific granule deficiency
respiratory burst:
Neutrophil immunodeficiency syndrome
Myeloperoxidase deficiency
Allergy / Immunology
Maternal–fetal medicine
Mass gathering medicine
Narcology
PM&R
Addiction psychiatry
Transplantation medicine
Bachelor of Medicine, Bachelor of Surgery
Bachelor of Medical Sciences
Master of Surgery
Doctor of Osteopathic Medicine
MD–PhD
Molecular oncology
Retrieved from "https://en.wikipedia.org/w/index.php?title=Neutropenia&oldid=935557723"
Monocyte and granulocyte disorders
Pages containing links to subscription-or-libraries content
Use dmy dates from December 2017


text:
is a multidirectional shooter developed by Sankindo and published by Face. The game was released in Japan for the PC Engine on July 12, 1991.

Plot
A female pilot is tasked with completing a VR training exercise and mastering the use of a prototype tank called the CS-05 Metal Stoker. However, during the course of the game, she discovers that she was actually fighting a real battle rather than performing in a simulation.

Gameplay

The player assumes the role of a female pilot (presented only in several cut-scenes) controlling a prototype CS-05 tank. The tank can move and shoot in any direction, it's also possible to strafe as the tank's firing position can be locked at any time by pressing the second action button. The action is presented in a top-down perspective and takes place across 7 large fields full of enemies and obstacles, with each level being composed of several sub-stages. Most of the levels feature free-directional scrolling areas allowing the players to seek out items and enemies however they wish. Progressing through a stage requires the fulfillment of a necessary condition (usually the destruction of a group of enemies or a mini-boss), an arrow and a Go! sign appear when the conditions are met. Scattered across the stages the players can find extra lives, energy refills and power pods which expand the tank's arsenal. There are five types of weapons available: a rapid firing machine gun, a double barreled laser beam, mines, homing missiles and a force field that damages anything that runs into it. Each weapon can be powered-up by collecting the same power pod twice and can also unleash a special attack (activated by pressing Select).

Development
Metal Stoker was created by Japanese video game developer Sankindo, who had previously worked on games like Cross Wiber and Time Cruise. Japanese film director Hiroyuki Nasu, famous for his award-winning film adaptation of the manga Bee Bop High School, was in charge of the game's direction and graphic design. The game's soundtrack was composed by Hiroto Saitou, who would later work on the PS3 adventure game Folklore (2007), and Nozomu Takahashi who, following the release of Metal Stoker,  became a production manager for Studio Ghibli.

Reception

ASM felt that Metal Stoker was well-made from a technical standpoint, commending its graphics, music and smooth scrolling. They also praised the game's difficulty curve arguing that its slowly increasing level of difficulty makes the game suitable for both beginners and experienced players. In their retrospective review, Hardcore Gaming 101 argued that Metal Stoker features elements that "help it stand out among the PC Engine's other overhead shooters today". They praised the graphics and soundtrack and highlighted the variety in the game's level design and scenery, noticing that "this variety is where the game consistently shines. (...) There will be a number of specific powerful foes you will usually need to destroy to progress, and every one of these areas (...) has a different look and enemies unique to them to keep things interesting".

See also
 Assault (1988)
 Bangai-O (1999)
 Bosconian (1981)
 Granada (1990)
 Last Mission (1986)
 Sinistar (1983)
 Thunder Force (1983)
Time Pilot '84 (1984)
Vanguard II (1984)

References

1991 video games
Face (company) games
Japan-exclusive video games
Multidirectional shooters
Single-player video games
TurboGrafx-16 games
TurboGrafx-16-only games
Video games about virtual reality
Video games developed in Japan
Video games featuring female protagonists


Very impressive use of rotoscoping helps create the highly original style of this great short. In a world where so much emphasis is put on 3D, the "flatness" of the visuals in this animated short is…refreshing! Director Andrew S. Allen did not even use shadows to simulate relief or depth perception. Instead, his creative use of contrasting textures and his smart choice of colours separate the various silhouettes and gives the impression of changes in light, which successfully sets the mood of the various scenes. An awesome short to watch full screen in HD.


At Newport, the Brewer Witches capped off an undefeated regular season by topping the Warriors.
Kenzie Dore homered in the top of the first to give Brewer a 1-0 lead. Libby Hewes led off the fourth with a double and scored on M.J. Sellars's groundout. Brewer scored four insurance runs in the fifth, aided by a two-run double by Hewes and three Nokomis errors.
The Warriors had a two-out rally in the bottom of the seventh when Alyssa Ellis and Britney Bubar had back-to-back doubles and each scored, but the rally ended on a ground out back to Hewes in the circle.


text:
Pages: 1 ... 7 8 [9] 10
Sorry! I missed this.
By all means and let me know what you need added, okay?
Thanks. I passed along your welcoming words to Third Eye. They seem to be still unsure what they're doing.
FlyingVProd
USC film school is rated as the best film school by the Hollywood Reporter...
https://www.hollywoodreporter.com/lists/top-25-american-film-schools-ranked-1231343/item/2019-top-25-film-schools-usc-1231345
NYU came in second.
And Chapman should be third, but instead they put Chapman as seventh.
Salute,
Tony V.
RIP Peter Fonda. His passage sadly noted here in the Black Hills. He often showed up at the bike rally in Sturgis. In many films I didn't see, but also in films I did see and liked, like Ulee's Gold, The Limey, and of course ER.
I noticed that Disney has made a new film, "Maleficent, Mistress of Evil," and there is a new film "Joker," and I wonder why they are all focusing on the villains instead of focusing on the good heroes like the old days.
Films are supposed to bring out the best in us, and they are supposed to give the kids good role models to look up to, like Roy Rogers, and the Lone Ranger, etc.
I need to start making movies, so that I can make movies my way.
Although, my film "Echo, A Rock and Roll Tragedy" will not be a good film for children, and will be rated "R."
I want to make a bunch of love stories. And really, "Echo" is a love story.
And I may make a movie about Lech Walesa, who is a hero.
We will see what I can do...
The late, great Andrzej Wajda beat you to it.
https://m.imdb.com/title/tt2113820/
Though I read the novel eons ago, I realized neither of us had seen the movie. Despite some mixed feelings about the sanitizing process Kubrick was forced into by production codes, the quality of the performances make it worth seeing, with memorable swerves between creepy and comedic. Shelley Winters is amazing as usual -- predatory, pretentious, volatile, and whiny. Though looking a bit run down at the end.
Given that Sue Lyon was 15 by the time production ended, and easily passed for 17 (movie Lolita is in high school, not the 11 year old of the novel), the pedophile aspect is considerably watered down.
Some reservations about Sellers in this - his "Quilty" often has the feel of an actor amusing cast and crew with over-the-top riffs on other movie roles where he does funny accents, rather than something that really belongs in the film. That Humbert can't recognize Quilty when he impersonates Dr. Strangelove a German psychologist is a bit improbable, and pulled me out of the movie a little. The scene where Quilty poses as a state policeman is just annoying and ridiculous.
RIP Lolita. Sue Lyon just died.
I'm thinking of aurochs and angels, the secret of durable pigments, prophetic sonnets, the refuge of art. And this is the only immortality you and I may share, Sue Lyon.
One of the great closing paragraphs of modern literature. Up there with the final line of Joyce's "The Dead."
There, in front of us, where a broken row of houses stood between us and the harbour, and where the eye encountered all sorts of stratagems, such as pale-blue and pink underwear cakewalking on a clothesline ... it was most satisfying to make out among the jumbled angles of roofs and walls, a splendid ship's funnel, showing from behind the clothesline as something in a scrambled picture – Find What the Sailor Has Hidden – that the finder cannot unsee once it has been seen.
You really can't have too much Nabokov in a forum.
Quote from: oilcan on December 29, 2019, 06:38:33 PM
FIFY.
Heh. I don't disagree.
This last lines should be Nabokov, but it's Milan Kundera....
"Up out of the lampshade, startled by the overhead light, flew a large nocturnal butterfly that began circling the room. The strains of the piano and violin rose up weakly from below."
Watched On the Beach, due to current events and curious how it held up some 55 years after its premiere.
The film is stiff and clunky in places, but it has its thought-provoking moments. The Rube Goldberg thing with the window shade and coke bottle was ridiculous but I have to give credit for a sort of existential joke there, as in we're all looking for signals of civilization in what can be random beeps. Ava Gardner, as always, mesmerizing, here as a hot mess who struggles with self loathing and Peck's shell of denial that his family is gone. Peck seems to have trouble, as an actor, hitting his marks, and somehow never fully grapples with the awfulness of what is coming down. Also, why must Australia must be represented by one song that is surely one that even tavern drunks have grown tired of? I would have enjoyed a scene where a bunch of roughnecks set upon the trout fishing crew that keep drinking and singing it endlessly.
Astaire's acting is underwhelming, though he does offer some ruminations on the folly of humankind and our penchant for wars that nobody really wants...his words still seem applicable. The Grand Prix race is rather hokey, and we are supposed to believe an egghead professor, if dropped into a well-tuned Ferrari, will emerge victorious. It's oddly dull and would have done no harm to the film if left in the cutting room. The film raises so many interesting possibilities of what people might do at the end of the world which are mostly unexplored. Maybe, in a sad way, that's the point: most opt for meekness and denial.
bodiddley
The Real Lolita
The story of 11-year-old Sally Horner's abduction changed the course of 20th-century literature. She just never got to tell it herself.
Interesting read which offers some analysis of the novel Lolita as well. I'd heard about this case which inspired Nabokov, but didn't know the full details or recall that it had occurred in NJ.
The read kind of makes you appreciate the Amber Alerts and priority put on child abduction these days.
It also put me in mind of the film An Education, though the girl there is 16 or 17, and not the 11 year Sally Horner of Camden or 13 year old Delores Haze of imaginative legend.
I should read Lolita again. This would probably be the 5th time, but when you know it so well, you can focus on the language or the unreliability of Humbert or the parenthetical asides, etc. without worrying about the narrative thrust (mild pun intended).


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The Stella explosion
by Greg O'Rourke | Apr 6, 2018 | Articles, General Updates | 0 comments
Today I want to share a recording I've been listening to a lot lately:
This is the Cuban band Irakere's unorthodox rendition of Stella By Starlight.
It has a funny story too:
Irakere seemingly got the name of Stella By Starlight mistranslated to "Stella Explosion". However, this accurately describes this "explosive" recording.
I'm a big fan of the electric playing from this band led by the acclaimed saxophonist Paquito D'Rivera. I particularly like the way Paquito D'Rivera plays the saxophone solo on this recording that you'll hear towards the start of this track.
In fact:
D'Rivera's solo has been a key influence in how I approach the improvisation tutorials in my new lesson series on Stella By Starlight. You can access the lessons by joining my Club if you're not part of it already.
Among various techniques, you'll learn simple tricks for playing fast double time lines, and how to solo over subdominant minor chords – one of the most common harmonic situations in jazz progressions.
Get the new lesson series here>>
P.S – I've been getting a lot of questions and interest about my upcoming jazz guitar improvisation course. To get notified of updates and news of when it will open it's doors for bookings, enter your details on this page to be the first to know:


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Q: Find a VPN Name on a Cisco ASA box given a remote IP address I have a Cisco ASA box with multiple site-to-site VPNs defined.
Is there a way to automate finding the VPN name based on a given remote IP address?

redpajama_set_name: RedPajamaStackExchange


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U-858 je bila nemška vojaška podmornica Kriegsmarine, ki je bila dejavna med drugo svetovno vojno.

Zgodovina
Podmornica se je predala 14. maja 1945 v Lewesu (Deleware). Potem ko so jo Američani uporabljali kot tarčo za preizkus torpedov, so jo decembra 1947 potopili.

Poveljniki

Tehnični podatki
| border="1" cellpadding="5" cellspacing="0" align="center"
|+Pregled karakteristik
|-
! style="background:#ffdead;" | Kategorije
! style="background:#ffdead;" | Podatki
|-
| Teža (t):na površjupod površjempolna Teža
| 1.1201.2321.545
|-
| Dolžina (m) - tlačni trup (m)
| 76,76 - 58,75
|-
| Širina (m) - tlačni trup (m)
| 6,86 - 4,44
|-
| Izpodriv (m)
| 4,67
|-
| Višina (m)
| 9,6
|-
| Moč (hp):na površjupod podvršjem
| 4.4001.000
|-
| Hitrost (vozlov):na površjupod podvršjem
| 197,3
|-
| Doseg (milja/vozel):na površjupod podvršjem
| 13.850/1063/4
|-
| Torpeda/Torpedne cevi
| 22/6 (4 na premcu, 2 na krmi)
|-
| Krovni top (mm)
| 105 (110 granat)
|-
| Mine
| 44 TMA
|-
| Posadka
| 48-56
|-
| Maksimalni potop (m)
| 230
|-
|}

Glej tudi

 seznam podmornic druge svetovne vojne
 seznam nemških podmornic druge svetovne vojne
 seznam vojaških plovil Kriegsmarine

Viri & Zunanje povezave
- v angleščini:
 UBoat.net
 ubootwaffe.net
- v nemščini:
 Lexikon der Wehrmacht

Nemške podmornice druge svetovne vojne
Podmornice razreda IXC/40


text:
Q: How to get input from cat over ssh in Perl I'm trying to cat a remote file over ssh and process it in local script line by line. So far I've tried this
open(INPUT,"| ssh user@host cat /dir1/dir2/file.dat")

but obviously it's only printing the file.dat to the STDOUT.
I know I can probably just scp the file and process it, but...

A: You're piping into ssh. I think you want to move the pipe to the other end so you can read the output from that cat command.

A: I would use
$file_contents = `ssh user@host cat /dir1/dir2/file.dat`;
@lines = split(/\n/, $file_contents);
.
.
. # process the file contents

That captures the output of the command (i.e. the contents of the file).


The LGBT2 Supervision Tips document was created by the Consortium's LGBT2 Constituency Committee to support LGBT2s and their supervisors in initiating, developing, and maintaining productive supervisory relationships. These tips were formed using information generated by the LGBT2 Supervision Tips Survey released in summer 2014. LGBT2s and their supervisors can use this document as a guide for conversations or as a reference tool in ways that best fit the unique supervisory relationship between these practitioners.
Join the members of the Consortium in the discussion! For more information about these tips, please connect with the LGBT2 Constituency Committee (LGBT2CC) by emailing [email protected]!
New York, N.Y. - We, The Consortium of Higher Education LGBT Resource Professionals, were disappointed to learn that the Creating Change conference initially allowed ICE to enter the conference space as an attendee and presenter. For many of us, this space has been held as sacred and inclusive, but ICE's inclusion in the conference has shown us otherwise. The Consortium of Higher Education LGBT Resource Professionals stands in solidarity with undocumented communities and attendees at this conference, including the students and staff that we bring to our conference and support on our campuses.
Ensure all LGBTQ undocumented immigrant attendees are supported financially throughout the conference (meals, lodging, registration, travel, etc).
The combined vision and mission of the Consortium of Higher Education LGBT Resource Professionals is to achieve higher education environments in which lesbian, gay, bisexual, and transgender students, faculty, staff, administrators, and alumni have equity in every respect. Our goals are to support colleagues and develop curriculum to professionally enhance this work; to seek climate improvement on campuses; and to advocate for policy change, program development, and establishment of LGBT Offices/Centers. Learn more about the Consortium at lgbtcampus.org.
New York, N.Y. – As college health programs work to support the overall goals of their institutions, providing trans-inclusive healthcare will enhance academic success for transgender students and comply with Title IX guidelines requiring educational institutions to not discriminate on the basis of gender identity in the provision of services. Likewise, under the Affordable Care Act, institutions are increasing emphasis on non-discrimination in healthcare on the basis of gender identity. The following document details recommendations designed to create climates and environments in college health that are inclusive and affirming of transgender, gender nonconforming, genderqueer, and similarly self-identified students. Students' gender expressions span a wide spectrum. The following recommendations can help mitigate barriers that trans students face when accessing mental health, physical health and preventative services on campus. The Consortium is grateful to partner with ACHA to release this document.
The document can be downloaded at ACHA.
New York, N.Y. – The Consortium of Higher Education LGBT Resource Professionals is seeking proposals for the Consortium Summer 2016 Institute and Board Retreat. Since 2009, the Consortium board has hosted a summer planning retreat at different campuses across the U.S. including University of Wisconsin-Madison and University at Albany-SUNY. As a benefit to the retreat site, we have also hosted a day-long summer institute for higher education professionals.
We are pleased to announce the Consortium's invitation for proposals to host the 2016 Summer Board Retreat and Day-Long Institute! Your campus could host a professional development opportunity for your region. Since the retreat travels, we would like to take the retreat and institute to a new location where it can benefit our members.
In the 2016 Institute Proposal document, you will find descriptions of the Institute, the Retreat and the partnership between the hosting institution and the Consortium. The detailed proposal form (pgs 4 - 7 of the attachment) includes an opportunity to outline proposed dates, venue(s), logistics and much more. Consider inviting the Consortium board to your campus in Summer 2016. The deadline for proposals is Monday, November 23, 2015! What questions do you have? Please contact D.A. Dirks and Matt Bruno for questions or more information.
The Common Application form evaluates prospective students for admission at over 500 colleges, universities and other organizations in 47 states and the District of Columbia and internationally. A growing numberof colleges and universities are asking students their gender identity and sexual orientation on admissions forms, either by having questions on their own application materials or by adding supplemental questions to their Common Application.
Institutions with gender identity and sexual orientation identity questions on their applications include Duke University, Elmhurst College, Elon University, MIT, Northeastern Illinois University, the Ohio State University, Purdue University, the University of Iowa, the University of Maryland, Connecticut College, the University of California system and all two-year colleges in California and Washington.
In 2011, The Consortium of Higher Education LGBT Resource Professionals and Campus Pride requested that changes to the Common Application include optional questions on gender identity and sexual orientation. The request was denied.


Download and Install Microsoft Office 2013 – Tag : Microsoft Office 2013 Filehippo, Download Office 2013, Microsoft Office 2013 For PC, Microsoft Office 2013 For Windows 10, Microsoft Office 2013 For Windows 8, Microsoft Office 2013 For Windows 8.1, Microsoft Office 2013 For Windows XP, Microsoft Office 2013 For Windows Vista, Microsoft Office 2013, Microsoft Office 2013 Download 2018, Microsoft Office 2013 For Windows, Microsoft Office 2013 Latest Version, Microsoft Office 2013 For Mac, Microsoft Office 2013 For Android, Microsoft Office 2013 For Linux, Microsoft Office 2013 For Apple mac OS, Microsoft Office 2013 For iOS | Microsoft Office 2013 next model of Office will be referred to as Office 2016 and its development is well underway. Both Mac and Windows customers have the ability to download the sneak peek variation for a quick spin with Microsoft committing to upgrading it rather regularly. While no dates have been established for a launch, expect it to be introduced within days of Windows 10 (whose launch day has been set for 29/7).
Note that there will certainly be 3 various versions of office: the conventional, customer one, one optimized for touch and also one for smart phones. Likewise, you won't have the ability to utilize Office 2016 as well as Office 2013 concurrently. You can inspect our Office 2016 preview here as well as a handy 50-tips guide.
Because this post was last upgraded (October 2014), Microsoft has issued more than one hundred updates for the different components of Office 2013 Find out more regarding those updates – including some security-related ones – here. June nonetheless saw a whopper one with more than 50 updates (indeed 50) in one release cycle, albeit for all the various Office SKUs. The original review is below.
Microsoft Office 2013 is the latest refresh of Microsoft's near common company collection. The most recent variation sees it obtain the Windows 8 treatment, with a touch-friendly user interface and a sparser appearance, along with new features in every application.
As part of the launch, Office has actually likewise gotten to a new landmark by going into the cloud with subscription pricing, on-demand installment as well as automated syncing of settings as well as documents you conserve on-line instead of offline. It's called Office 365.
That particular technique of acquisition guarantees you're constantly updated, even if the software application modifications – take a look at our Office 365 evaluation.
Download Microsoft Office 2013 – While the main thing you'll notice with Office 2013 the makeover, there are some really interesting features under the hood – though regretfully not for Windows XP individuals, that are now omitted. Office 2013 is purely for Windows 7 as well as Windows 8 individuals.
As usual, there are multiple variations of Office 2013, yet this moment around the different versions are not almost whether you're utilizing them in your home or in a service or which applications are included.
Even if you decide you intend to acquire a pay-for-it-once-and-keep-it duplicate of Office 2013 in a box, you will not find a DVD inside– just a product secret to open the software application you download and install. (Customers in "establishing countries with minimal internet accessibility" can still get a DVD, yet that's not an alternative in the UK or US.).
If you favor to pay a yearly registration to obtain additional functions, Office 365 editions allow you download the Office 2013 applications onto multiple PCs (or share them with your household).
For house users, there are 4 options. Acquire the boxed software and also you could place it on one PC. Office House and Trainee 2013 with Word, Excel, PowerPoint as well as OneNote expenses ₤ 109.99/$ 139.99; Office Home and Business 2013 adds Outlook as well as prices ₤ 219.99/$ 219.99. Office Specialist 2013 has the complete collection of programs for ₤ 389.99/$ 399.99; Word, Excel, PowerPoint, OneNote, Outlook, Accessibility and Author.
Additionally, as you could expect, Office 2013 and Office on Demand only operate on Windows 7 as well as 8, not on XP or View.


Find Homes and Land for sale in and around Uniontown PA Use the MLS search feature below to find homes and land for sale in Fayette County PA including neighborhoods of Uniontown, Craig Meadows, Cross Creek, Fairchance, Farmview, Heritage Hills, Hopwood, Regency Forest, South Union, North Union, Ruby View, Smithfield, Mountain Area - Laurel Highlands, Farmington, Wharton, Chalk Hill, Henry Clay, Markleysburg, Nemacolin Woodlands, Gibbons Glade, Belle Vernon, Confluence and Ohiopyle.
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On a lovely fall day, the North Hills Country Club was full to the brim with guests celebrating the marriage of Colleen and Kevin. From the tallest to the smallest in their family, everyone agreed that these two are the perfect match. And when you witness the excitement and happiness that they radiated on their special day, you will certainly agree!
A light dusting of snow covered the ground to welcome guests to Amanda and Tyler's wedding. Sentryworld was the perfect destination for these two to declare their love in front of family and friends. After braving the cold and blowing bubbles at the new couple, everyone partied the rest of the night away upstairs.
Kathryn and Philip chose the Wisconsin Club as the setting for their romantic wedding day. The vintage look and beauty of the venue provided the perfect backdrop for the couple's ceremony and reception. Kathryn and Philip were already legally wed, but wanted to make their union even more special by declaring their love in front of family and friends. There wasn't a dry eye in the house as the couple exchanged their custom vows. Thank you Kathryn and Philip for letting us be a part of your wonderful day.


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Industry Partners & Recruiters
Harnessing bacteria to fight ocean pollution
Students' synthetic biology project claims gold at international competition
By Adam Zewe | Press contact
Plastic water bottles that end up as litter contribute many of the 5 trillion tiny plastic pieces that are polluting the world's oceans. (Photo courtesy of the National Oceanic and Atmospheric Administration.)
More than five trillion tiny pieces of plastic are floating on the surface of the world's oceans, threatening marine life and posing health hazards to humans. Bacteria could help researchers detect and clean up these plastic fragments, according to research conducted by a group of Harvard undergraduates.
The students, members of the International Genetically Engineered Machine (iGEM) team at the John A. Paulson School of Engineering and Applied Sciences (SEAS), used synthetic biology to tackle the problem of marine micro-plastic pollution. Their solution, Plastiback, won a gold medal at the annual iGEM competition, which included more than 300 student teams from dozens of nations. It was also nominated for best environmental project in the undergraduate category.
The Harvard team at the International Genetically Engineered Machine (iGEM) Competition championship in Boston. (Photo courtesy of the Harvard iGEM team.)
"Ocean researchers don't currently have a very good way of quantifying or detecting where these tiny pieces of plastic may be in the ocean," said team member Lorena Lyon, A.B. '18, a human development and regenerative biology concentrator. "Current methods typically involve trawling the ocean, which means researchers go out with a net and physically count the pieces of plastic they pick up. That is not very efficient."
Plastiback is a floating ocean bioreactor containing genetically modified E. coli bacteria, which is used to identify and degrade a type of micro-plastic called polyethylene terephthalate (PET). PET is commonly used in many products that end up as ocean pollution, from disposable water bottles to commercial fishing equipment. The students genetically engineered these E. coli to secrete an enzyme that breaks down PET into terephthalic acid and ethylene glycol. A fluorescent protein glows green when the PET-degrading enzyme is expressed, so researchers who use the device can easily see whether the system is working correctly.
"Degrading plastic in the ocean leads to less plastic, which is a great thing up to that point, but the byproducts of plastic are harmful, so we added another step to our project to extend that idea of environmental remediation," said Kevin Shani, S.B. '18, a bioengineering concentrator.
During the next step, the terephthalic acid produced during degradation flows into a microbial fuel cell located in another chamber of the floating bioreactor. A second type of bacteria, D. tsuruhatensis sp. nov., metabolizes the acid, producing electrons that are used to generate a small amount of voltage. Researchers could measure the voltage to quantify how much PET was broken down by Plastiback.
This diagram shows how the Plastiback process works. (Image courtesy of the Harvard iGEM team.)
Not only would Plastiback help degrade harmful plastic in the oceans, but its detection system could also enable researchers to create an ocean map pinpointing "hot spots" where large concentrations of micro-plastic exist, Lyon explained.
"These micro-plastics are so dangerous because they can absorb toxins, like DDT, and then be consumed by fish," said Yasmeen Fakhro, A.B. '18, a chemistry and physics concentrator. "If those fish are eaten by humans, that poses a huge risk to public health."
A recent study published in Nature found that more than 25 percent of fish for sale in public markets contained micro-plastic. Identifying large collections of micro-plastic could help fishermen and food regulators recognize public health dangers that may be present in each catch.
As part of the iGEM competition, the students submitted the E. coli genetic constructs they developed to the Registry of Standard Biological Parts, making it possible for researchers around the world to access the data and apply it to their own research.
While certain parts of the project had a steep learning curve, such as retrofitting a microbial fuel cell, working as a team to tackle an important environmental issue was rewarding, said Daniel Um, A.B. '19, an integrative biology concentrator pursuing a secondary in computer science.
"This field has been growing exponentially over the past decade," he said. "It is exciting to be part of this wave of synthetic biology research. I'm proud of what we were able to accomplish."
The team, which began working on their project over the summer, also included students Gabriela Berner, Will Cho, Rebekah Chun, and Mofeyifoluwa Edun. They were mentored by Neel Joshi, Associate Professor of Chemical and Biological Engineering, and graduate students Pichet Praveschotinunt, David Lips, Kevin Hof, and Anita Chandrahas.
"The team deserves a lot of credit for designing, planning, executing, and presenting a project that they conceived primarily on their own, with some help from the mentoring team," said Joshi. "I hope that the success of this year's team can continue to attract more undergraduates to participate in the team and in the student group started by last year's team."
Get involved with iGEM
Harvard undergraduates who are interested in getting involved with iGEM on campus can contact crimsonigem@gmail.com. In addition to participating on the summer iGEM research team, the Harvard College iGEM club offers opportunities to visit biotech companies, hear world-class speakers, and pursue student-directed research. This Wintersession, the club is hosting a BioDesign Bootcamp in which students can discover synthetic biology (no prior experience necessary). Apply now at http://bootcamp.harvardigem.com.
Topics: Bioengineering
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Locking up fats in CAGEs to treat obesity
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