Associate Research Scientist I (Protein Expression and Production)
$59K-$116K (Glassdoor est.)
Position Description

The successful candidate will be highly proficient in recombinant protein expression and production by using various host cell systems (e.g. Mammalian, BEVS, and E coli). Responsible for the generation of milligram to sub-gram scale quantities of recombinant proteins to support small molecules high throughput screen.

Position Requirements

ESSENTIAL DUTIES AND RESPONSIBILITIES:
• Conduct protein expression from various shake flask scales to scale up with bioreactor systems using Mammalian/E coli/BEVS cultures.
• Responsible for operating and maintenance of all fermentation as well as cell culture equipment. Stocking Protein Expression supplies and media.
• Experimentally determine methods for optimizing yield and other process parameters for protein expression.
• Perform protein pull-down using relevant protein/resin interaction and protein chemistry.
• Prepares and organizes data for presentation using analysis and visualization software; presents data and reports on project status at individual, group, and departmental research meetings under general supervision.
• Maintain familiarity with current scientific literature relevant to the research experiments or program
• Maintain laboratory notebook in a complete, consistent, and concise manner in accordance with company intellectual property policies and practices.
• Perform additional tasks or assist with special projects as assigned or needed.
• Adhere to good health and safety practices in compliance with applicable EH&S rules and participate in mandatory safety training programs.

SUPERVISORY RESPONSIBILITIES:
• None

EDUCATION/EXPERIENCE/SKILLS:
Education:
• Bachelor’s degree (B.A./B.S.) in related discipline and four to six years of relevant experience; or
• Master’s degree (M.A./M.S.) in related discipline and two to four years of relevant experience; or
• Equivalent combination of education and experience.

Experience:
• Extensive experience with cell cultures (HEK293 and E coli a must, insect cell desirable).
• Demonstrated expertise in the execution of protein expression at bench as well as scale-up production in WAVE Bioreactor.
• Expert level operator of WAVE Bioreactor system. Proficient at method development with Unicorn.
• Experience with recombinant protein purification (protein pulldown with appropriate protein/resin interaction and protein chemistry) is a plus.
• Experience in metabolite-detection assay as well as protein characterization (SDS-PAGE, and Western Blotting, etc.) is a desired.
• Proficiency with computer software including MS Word, MS PowerPoint, MS Excel is desired.
Knowledge/Skills/Abilities:
• Working knowledge of molecular and cellular biology techniques as applied to protein production.
• Ability to work independently while following protocols.
• Complete understanding and wide application of scientific principles, theories and concepts in field of specialty as well as general understanding and knowledge in other related disciplines
• Ability to read and interpret documents such as safety rules, operating and maintenance instructions, procedure manuals and scientific articles.
• Ability to write routine reports and correspondence.
• Strong communication skills including effective public speaking before groups and individuals from other departments. Ability to effectively capture data and results.
• Excellent work ethic, strong self-motivation, and ability to work effectively in a either a team environment or independently.
• Strong collaboration skills with other team members.

JOB COMPLEXITY:
• Works on problems of diverse scope where analysis of data requires evaluation of identifiable factors.
• Exercises judgment within generally defined practices and policies in selecting methods and techniques for obtaining solutions.
• Uses professional concepts and company policies and procedures to solve a wide range of difficult problems in imaginative and practical ways.

DISCLAIMER
The preceding job description has been designed to indicate the general nature and level of work performed by employees within this classification. It is not designed to contain or be interpreted as a comprehensive inventory of all duties, responsibilities and qualifications required of employees assigned to the job.

We are an Equal Opportunity Employer and do not discriminate against any employee or applicant for employment because of race, color, sex, age, national origin, religion, sexual orientation, gender identity, status as a veteran, and basis of disability or any other federal, state or local protected class.
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