Sannan has been using materials left behind by Kodo to continue to try to improve the medicine in secret. He drinks the medicine and transforms into something which is no longer human. After seeing the wound Sannan infected on Chizuru heal in an instant, Hijikata asks her if she's a rasetsu, but...
Later, the commotion over Sannan adds to the decision to move the Shinsengumi headquarters to Nishi Hongwanji. Chizuru continues to search for her father, but she is unable to find any clues to his whereabouts. Then one day, Chizuru happens to overhear Okita's secret.
The research on the Water of Life is secret.
البحث حول ماء الحياة سرّي
Ich habe am Wasser des Lebens geforscht.
El Agua de Vida es alto secreto.
El Agua de Vida es alto secreto.
L’eau de jouvence
est confidentielle.
Le ricerche sull'Acqua della Vita 
sono un segreto.
A pesquisa da Água da Vida é secreta.
Изучение живой воды
проводится тайно.