The substitutes begin operating as members of the Shinsengumi. Their first job is escorting Sakuma Shozan. However, something seems to have happened between Sakuya and Shozan in the past...
"The Shinsengumi disposed of 
a group of villainous ronin
"La Shinsengumi si è occupata
di un gruppo di ronin malvagi
"O Shinsengumi eliminou um
grupo de samurais malignos
Синсэнгуми устранила