The substitutes begin operating as members of the Shinsengumi. Their first job is escorting Sakuma Shozan. However, something seems to have happened between Sakuya and Shozan in the past...
What about you, rooster head? 
Will you abandon the sword?
Und du Stachelkopf?
Was wirst du tun?
¿Y tú, pelos puntiagudos?
¿Dejarás la espada?
¿Y tú, pelo de pincho?
¿Dejarás la espada?
Et toi, aux cheveux pointus ?
Tu abandonnerais ton sabre ?
E tu, testa a punta? Abbandonerai la spada?
E você, cabeça de galo?
Vai abandonar sua espada?
Ну а ты что, острохвостый?
Откажешься от меча?